DE3854470T2 - Verfahren für Affinitätsuntersuchungen durch Verwendung von Ziel-Amplifizierung. - Google Patents

Verfahren für Affinitätsuntersuchungen durch Verwendung von Ziel-Amplifizierung.

Info

Publication number
DE3854470T2
DE3854470T2 DE3854470T DE3854470T DE3854470T2 DE 3854470 T2 DE3854470 T2 DE 3854470T2 DE 3854470 T DE3854470 T DE 3854470T DE 3854470 T DE3854470 T DE 3854470T DE 3854470 T2 DE3854470 T2 DE 3854470T2
Authority
DE
Germany
Prior art keywords
procedure
target amplification
target
affinity testing
affinity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3854470T
Other languages
English (en)
Other versions
DE3854470D1 (de
Inventor
Mark Leo Collins
Donald Neil Halbert
Walter King
Jonathan Michael Lawrie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP Corp North America Inc
Original Assignee
BP Corp North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP Corp North America Inc filed Critical BP Corp North America Inc
Publication of DE3854470D1 publication Critical patent/DE3854470D1/de
Application granted granted Critical
Publication of DE3854470T2 publication Critical patent/DE3854470T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00445—Other cooling arrangements

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE3854470T 1987-12-21 1988-12-21 Verfahren für Affinitätsuntersuchungen durch Verwendung von Ziel-Amplifizierung. Expired - Lifetime DE3854470T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13692087A 1987-12-21 1987-12-21

Publications (2)

Publication Number Publication Date
DE3854470D1 DE3854470D1 (de) 1995-10-19
DE3854470T2 true DE3854470T2 (de) 1996-03-28

Family

ID=22475015

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3854470T Expired - Lifetime DE3854470T2 (de) 1987-12-21 1988-12-21 Verfahren für Affinitätsuntersuchungen durch Verwendung von Ziel-Amplifizierung.

Country Status (5)

Country Link
EP (1) EP0328829B1 (de)
JP (1) JP2817926B2 (de)
AT (1) ATE127854T1 (de)
AU (1) AU2735988A (de)
DE (1) DE3854470T2 (de)

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US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
AU623602B2 (en) * 1987-06-19 1992-05-21 Akzo Nobel N.V. Transcription-based nucleic acid amplification/detection systems
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
EP0447464B1 (de) * 1988-12-09 1998-08-05 Amrad Corporation Limited Amplifizierter dns-assay
DE69030804T2 (de) * 1989-04-05 1997-11-06 Vysis Inc Reagenzien zur verbesserung von hybridisierung
ES2045932T3 (es) * 1989-06-12 1994-01-16 Cis Bio Int Procedimiento para la deteccion de secuencias especificas de acidos nucleicos y sus aplicaciones.
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
WO1991015599A1 (en) * 1990-04-05 1991-10-17 Amrad Corporation Limited A method for detecting dna
CA2080019A1 (en) * 1990-04-06 1991-10-07 Edward G. Jablonski Process and composition for performing dna assays
ES2101867T3 (es) * 1990-05-07 1997-07-16 Daikin Ind Ltd Aplicaciones diagnosticas de la formacion de lazos d dobles.
RU2017821C1 (ru) * 1990-10-10 1994-08-15 Анатолий Михайлович Онищенко Способ амплификации днк и устройство для его осуществления
EP0533952A4 (en) * 1991-04-15 1993-07-07 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
WO1992020820A1 (en) * 1991-05-15 1992-11-26 Vanderbilt University Method to determine metastatic potential of tumor cells
US5437976A (en) * 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
WO1993022020A2 (en) * 1992-04-23 1993-11-11 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US5312730A (en) * 1992-05-27 1994-05-17 Ciba Corning Diagnostics Corp. Immune complex transfer with lypophilic bridge
DE69327454T2 (de) * 1992-10-09 2000-05-11 Vysis Inc Analytisches verfahren
AU707391B2 (en) * 1994-05-28 1999-07-08 Tepnel Medical Limited Producing copies of nucleic acids
CA2285918C (en) * 1997-04-04 2007-07-03 Biosite Diagnostics Methods for concentrating ligands using magnetic particles
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
AU738708B2 (en) * 1997-05-02 2001-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
AU2014277869B2 (en) * 1998-05-01 2015-04-30 Gen-Probe Incorporated System and method for incubating the contents of a reaction receptacle
DK1614475T3 (da) * 1998-05-01 2007-09-17 Gen Probe Inc Indretning til omröring af væskeindholdet i en beholder
AU2012232986B2 (en) * 1998-05-01 2014-06-12 Gen-Probe Incorporated System and method for incubating the contents of a reaction receptacle
DE60137104D1 (de) 2000-03-31 2009-02-05 Sanko Junyaku Kk Sonde zur herstellung einer polymersonde, verfahren zur herstellung einer polymersonde sowie verwendung derselben
FR2811321A1 (fr) * 2000-07-04 2002-01-11 Bio Merieux Amplificateur d'une region ribonucleique cible d'un arn ribosomal 16s ou adn pour un tel arn d'une espece eubacterienne et detection de telles especes
US20030148273A1 (en) * 2000-08-26 2003-08-07 Shoulian Dong Target enrichment and amplification
DE10119468A1 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-Verfahren zur Anreicherung von DNA-Fragmenten aus komplexen Mischungen
US8030465B2 (en) 2001-06-29 2011-10-04 Medimolecular Pty Ltd Nucleic acid ligands to complex targets
WO2003045310A2 (en) * 2001-11-21 2003-06-05 Applera Corporation Digital assay
US8349276B2 (en) 2002-09-24 2013-01-08 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
EP3159417B1 (de) * 2006-08-01 2021-10-06 Gen-Probe Incorporated Verfahren zur nichtspezifischen zielerfassung von nukleinsäuren
EP2076609A1 (de) 2006-10-10 2009-07-08 Illumina Inc. Zusammensetzungen und verfahren zur repräsentativen auswahl von nukleinsäuren aus komplexen gemischen mittels hybridisierung
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP2053132A1 (de) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Anreicherung und Sequenzanalyse von Genombereichen
DE102007056398A1 (de) * 2007-11-23 2009-05-28 Febit Holding Gmbh Flexibles Extraktionsverfahren für die Herstellung sequenzspezifischer Molekülbibliotheken
US20100331204A1 (en) * 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
EP2746777A3 (de) 2010-07-23 2014-08-27 Beckman Coulter, Inc. System oder Verfahren zur Aufnahme analytischer Einheiten
WO2013070740A1 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Aliquotter system and workflow
ES2729283T3 (es) 2011-11-07 2019-10-31 Beckman Coulter Inc Sistema de centrífuga y flujo de trabajo
EP2776845B1 (de) 2011-11-07 2020-11-04 Beckman Coulter, Inc. Roboterarm
KR20140092378A (ko) 2011-11-07 2014-07-23 베크만 컬터, 인코포레이티드 샘플을 처리하기 위한 시스템 및 방법
BR112014011044A2 (pt) 2011-11-07 2017-04-25 Beckman Coulter Inc amortecimento magnético para sistema de transporte de espécime
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
CN105164279B (zh) * 2013-04-25 2021-02-02 萤火虫生物股份有限公司 靶核酸的多重分析
US10550440B2 (en) 2016-02-26 2020-02-04 Arizona Board Of Regents On Behalf Of Arizona State University Synthetic translation-sensing riboswitches and uses thereof
JP7232438B2 (ja) * 2018-04-27 2023-03-03 国立大学法人 東京大学 メチル含有基修飾核酸を精製、分離または濃縮する方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3586689T2 (de) * 1984-02-28 1993-04-08 Ortho Diagnostic Systems Inc Diagnose von genmissbildungen durch restriktionskarte-analysierung unter anwendung eines sandwichhybridisierungsformats.
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
GB8507706D0 (en) * 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
AU6403886A (en) * 1985-09-18 1987-04-07 Yale University Reca nucleoprotein filament and methods
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays

Also Published As

Publication number Publication date
AU2735988A (en) 1989-07-13
DE3854470D1 (de) 1995-10-19
JP2817926B2 (ja) 1998-10-30
EP0328829A3 (en) 1990-09-19
JPH01211500A (ja) 1989-08-24
EP0328829B1 (de) 1995-09-13
ATE127854T1 (de) 1995-09-15
EP0328829A2 (de) 1989-08-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN