DE3379665D1 - Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms - Google Patents

Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms

Info

Publication number
DE3379665D1
DE3379665D1 DE8383200712T DE3379665T DE3379665D1 DE 3379665 D1 DE3379665 D1 DE 3379665D1 DE 8383200712 T DE8383200712 T DE 8383200712T DE 3379665 T DE3379665 T DE 3379665T DE 3379665 D1 DE3379665 D1 DE 3379665D1
Authority
DE
Germany
Prior art keywords
modified
forms
preprothaumatin
yeast
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8383200712T
Other languages
German (de)
English (en)
Inventor
Luppo Edens
Adrianus Marinus Ledeboer
Cornelis Theodorus Verrips
Den Berg Johannes Abel Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever PLC
Unilever NV
Original Assignee
Unilever PLC
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19839766&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3379665(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Unilever PLC, Unilever NV filed Critical Unilever PLC
Application granted granted Critical
Publication of DE3379665D1 publication Critical patent/DE3379665D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pyrane Compounds (AREA)
DE8383200712T 1982-05-19 1983-05-19 Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms Expired DE3379665D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8202091 1982-05-19

Publications (1)

Publication Number Publication Date
DE3379665D1 true DE3379665D1 (en) 1989-05-24

Family

ID=19839766

Family Applications (2)

Application Number Title Priority Date Filing Date
DE8383200712T Expired DE3379665D1 (en) 1982-05-19 1983-05-19 Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms
DE8383200714T Expired - Lifetime DE3381090D1 (de) 1982-05-19 1983-05-19 Klonierungssystem fuer kluyveromyce spezies.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8383200714T Expired - Lifetime DE3381090D1 (de) 1982-05-19 1983-05-19 Klonierungssystem fuer kluyveromyce spezies.

Country Status (20)

Country Link
US (2) US4657857A (https=)
EP (2) EP0096430B2 (https=)
JP (3) JPS59501572A (https=)
AR (1) AR241796A1 (https=)
AT (2) ATE49421T1 (https=)
AU (1) AU571181B2 (https=)
BR (1) BR8307525A (https=)
CA (1) CA1224735A (https=)
DE (2) DE3379665D1 (https=)
DK (1) DK173699B1 (https=)
ES (1) ES522556A0 (https=)
FI (1) FI81379C (https=)
GR (1) GR78852B (https=)
HU (1) HU204097B (https=)
IE (1) IE55817B1 (https=)
IL (1) IL68733A (https=)
NO (1) NO172397C (https=)
NZ (1) NZ204302A (https=)
PT (1) PT76727B (https=)
WO (2) WO1983004050A1 (https=)

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JPH0716432B2 (ja) * 1982-10-20 1995-03-01 サントリー株式会社 酵母の高発現ベクタープラスミドを用いたペプチドの生産方法
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US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
DE3480006D1 (en) * 1983-05-19 1989-11-09 Unilever Nv Improvements in the expression of newly introduced genes in yeast cells
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US6214577B1 (en) 1984-05-22 2001-04-10 Robert Rogers Yocum Yeast vectors conferring antibiotic resistance
US5422267A (en) * 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
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US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
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US5047340A (en) * 1985-06-05 1991-09-10 The University Of Kentucky Research Foundation LAC+ saccharomyces cerevisiae
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EP0260404B1 (en) * 1986-08-21 1995-05-31 VAN DEN BERG, Robert Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes
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FR2649991B2 (fr) 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system
AU638438B2 (en) 1989-02-24 1993-07-01 Monsanto Technology Llc Synthetic plant genes and method for preparation
US20010003849A1 (en) 1989-08-07 2001-06-14 Kenneth A. Barton Expression of genes in plants
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US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
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US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
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ES2154995B1 (es) * 1998-11-20 2001-11-16 Consejo Superior Investigacion Levadura panadera recombinante capaz de asimilar lactosa.
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Also Published As

Publication number Publication date
AU1553983A (en) 1983-12-02
FI840149L (fi) 1984-01-16
JP2608540B2 (ja) 1997-05-07
JPS59500848A (ja) 1984-05-17
NO840133L (no) 1984-01-13
GR78852B (https=) 1984-10-02
ES8402874A1 (es) 1984-03-01
FI840149A0 (fi) 1984-01-16
AU571181B2 (en) 1988-04-14
PT76727B (en) 1985-12-27
DK11384A (da) 1984-01-11
EP0096430A1 (en) 1983-12-21
JPS59501572A (ja) 1984-09-06
ES522556A0 (es) 1984-03-01
US4657857A (en) 1987-04-14
DK173699B1 (da) 2001-07-02
IL68733A (en) 1989-01-31
EP0096910A1 (en) 1983-12-28
EP0096910B1 (en) 1989-04-19
JPS61275227A (ja) 1986-12-05
DE3381090D1 (de) 1990-02-15
ATE42338T1 (de) 1989-05-15
WO1983004050A1 (en) 1983-11-24
DK11384D0 (da) 1984-01-11
PT76727A (en) 1983-06-01
NO172397C (no) 1993-07-14
WO1983004051A1 (en) 1983-11-24
JPH0685720B2 (ja) 1994-11-02
FI81379B (fi) 1990-06-29
EP0096430B2 (en) 1998-04-01
FI81379C (fi) 1990-10-10
CA1224735A (en) 1987-07-28
NZ204302A (en) 1987-03-06
NO172397B (no) 1993-04-05
HU204097B (en) 1991-11-28
AR241796A1 (es) 1992-12-30
EP0096430B1 (en) 1990-01-10
IE55817B1 (en) 1991-01-30
IE831185L (en) 1983-11-19
ATE49421T1 (de) 1990-01-15
US4859596A (en) 1989-08-22
JPH0543348B2 (https=) 1993-07-01
BR8307525A (pt) 1984-08-21

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