DE3246090A1 - Verfahren zur quantitativen bestimmung von phosphatidyl-glycerin - Google Patents
Verfahren zur quantitativen bestimmung von phosphatidyl-glycerinInfo
- Publication number
- DE3246090A1 DE3246090A1 DE19823246090 DE3246090A DE3246090A1 DE 3246090 A1 DE3246090 A1 DE 3246090A1 DE 19823246090 DE19823246090 DE 19823246090 DE 3246090 A DE3246090 A DE 3246090A DE 3246090 A1 DE3246090 A1 DE 3246090A1
- Authority
- DE
- Germany
- Prior art keywords
- quantitative determination
- glycerol
- glycerine
- phosphatidyl
- phosphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 238000000034 method Methods 0.000 title claims description 36
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- ATBOMIWRCZXYSZ-XZBBILGWSA-N [1-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] (9e,12e)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C\C\C=C\CCCCC ATBOMIWRCZXYSZ-XZBBILGWSA-N 0.000 claims description 22
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- KNJDBYZZKAZQNG-UHFFFAOYSA-N lucigenin Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.C12=CC=CC=C2[N+](C)=C(C=CC=C2)C2=C1C1=C(C=CC=C2)C2=[N+](C)C2=CC=CC=C12 KNJDBYZZKAZQNG-UHFFFAOYSA-N 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- KSWSEONDYPYRDT-UHFFFAOYSA-N oxalic acid;2,4,6-trichlorophenol Chemical compound OC(=O)C(O)=O.OC1=C(Cl)C=C(Cl)C=C1Cl.OC1=C(Cl)C=C(Cl)C=C1Cl KSWSEONDYPYRDT-UHFFFAOYSA-N 0.000 description 1
- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
- 230000009984 peri-natal effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
- 238000000016 photochemical curing Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- HFQQZARZPUDIFP-UHFFFAOYSA-M sodium;2-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HFQQZARZPUDIFP-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- HPGGPRDJHPYFRM-UHFFFAOYSA-J tin(iv) chloride Chemical compound Cl[Sn](Cl)(Cl)Cl HPGGPRDJHPYFRM-UHFFFAOYSA-J 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- WRPWWVNUCXQDQV-UHFFFAOYSA-N vanillylamine Chemical compound COC1=CC(CN)=CC=C1O WRPWWVNUCXQDQV-UHFFFAOYSA-N 0.000 description 1
- 229940053939 vanillylamine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N2405/04—Phospholipids, i.e. phosphoglycerides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56203074A JPS58107199A (ja) | 1981-12-16 | 1981-12-16 | ホスフアチジルグリセロ−ルの定量方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3246090A1 true DE3246090A1 (de) | 1984-02-16 |
| DE3246090C2 DE3246090C2 (enrdf_load_stackoverflow) | 1991-10-10 |
Family
ID=16467912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19823246090 Granted DE3246090A1 (de) | 1981-12-16 | 1982-12-13 | Verfahren zur quantitativen bestimmung von phosphatidyl-glycerin |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS58107199A (enrdf_load_stackoverflow) |
| CA (1) | CA1201047A (enrdf_load_stackoverflow) |
| DE (1) | DE3246090A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2518117B1 (enrdf_load_stackoverflow) |
| GB (1) | GB2111677B (enrdf_load_stackoverflow) |
| IT (1) | IT1155384B (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0090339A3 (de) * | 1982-03-27 | 1984-10-10 | Roche Diagnostics GmbH | Verfahren und Reagens zur enzymatischen Bestimmung von Phosphatidylglycerin |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59140900A (ja) * | 1983-01-28 | 1984-08-13 | Toyo Jozo Co Ltd | 新規な酵素的高感度測定法 |
| EP0230343A3 (en) * | 1986-01-06 | 1988-08-10 | Isolab, Inc. | Method of determining the amount of phosphatidylglycerol in amniotic fluids as a diagnostic indicator |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2635040A1 (de) * | 1975-08-05 | 1977-02-17 | Hycel Inc | Reaktiv fuer die hydrolyse eines fettsaeure-glyzerinesters sowie verfahren zur analyse einer einen fettsaeure- glyzerinester enthaltenden waessrigen fluessigkeit |
| DE2737513A1 (de) * | 1976-08-19 | 1978-02-23 | Kyowa Hakko Kogyo Kk | Analysensatz zur bestimmung von phospholipiden und seine verwendung |
| US4241178A (en) * | 1978-01-06 | 1980-12-23 | Eastman Kodak Company | Process and composition for the quantification of glycerol ATP and triglycerides |
| DE3242652A1 (de) * | 1981-11-20 | 1984-01-19 | Toyo Jozo Co., Ltd., Shizuoka | Verfahren zur quantitativen bestimmung von phosphatidyl-glycerin |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS604716B2 (ja) * | 1976-04-26 | 1985-02-06 | 東洋醸造株式会社 | 新規なコリンオキシダーゼおよびその製法 |
| DE2938737A1 (de) * | 1978-09-26 | 1980-04-03 | Toyo Jozo Kk | Vorrichtung und verfahren zur gleichzeitigen analytischen bestimmung von mehreren komponenten in einer probe mittels enzymatischer analyse |
| JPS55111800A (en) * | 1979-02-20 | 1980-08-28 | Toyo Jozo Co Ltd | Kit for analysis of lipid component |
| SU831129A1 (ru) * | 1979-07-24 | 1981-05-23 | Дальневосточный Государственный Уни-Верситет | Способ получени фосфатидилглицерина |
| JPS5898096A (ja) * | 1981-12-07 | 1983-06-10 | Toyo Jozo Co Ltd | エタノ−ルアミン含有液の定量法 |
-
1981
- 1981-12-16 JP JP56203074A patent/JPS58107199A/ja active Pending
-
1982
- 1982-11-23 GB GB08233333A patent/GB2111677B/en not_active Expired
- 1982-11-29 CA CA000416607A patent/CA1201047A/en not_active Expired
- 1982-12-03 FR FR8220269A patent/FR2518117B1/fr not_active Expired
- 1982-12-13 DE DE19823246090 patent/DE3246090A1/de active Granted
- 1982-12-15 IT IT24763/82A patent/IT1155384B/it active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2635040A1 (de) * | 1975-08-05 | 1977-02-17 | Hycel Inc | Reaktiv fuer die hydrolyse eines fettsaeure-glyzerinesters sowie verfahren zur analyse einer einen fettsaeure- glyzerinester enthaltenden waessrigen fluessigkeit |
| DE2737513A1 (de) * | 1976-08-19 | 1978-02-23 | Kyowa Hakko Kogyo Kk | Analysensatz zur bestimmung von phospholipiden und seine verwendung |
| US4241178A (en) * | 1978-01-06 | 1980-12-23 | Eastman Kodak Company | Process and composition for the quantification of glycerol ATP and triglycerides |
| DE3242652A1 (de) * | 1981-11-20 | 1984-01-19 | Toyo Jozo Co., Ltd., Shizuoka | Verfahren zur quantitativen bestimmung von phosphatidyl-glycerin |
Non-Patent Citations (1)
| Title |
|---|
| DE-B.: BERGMEYER, H.U.: Methoden der enzyma- tischen Analyse, 3. Aufl., 1974, Verlag Chemie Weinheim/Bergstr., Bd. I, S. 537-538 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0090339A3 (de) * | 1982-03-27 | 1984-10-10 | Roche Diagnostics GmbH | Verfahren und Reagens zur enzymatischen Bestimmung von Phosphatidylglycerin |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2518117B1 (fr) | 1986-10-10 |
| IT8224763A1 (it) | 1984-06-15 |
| JPS58107199A (ja) | 1983-06-25 |
| GB2111677B (en) | 1985-01-16 |
| IT1155384B (it) | 1987-01-28 |
| GB2111677A (en) | 1983-07-06 |
| FR2518117A1 (fr) | 1983-06-17 |
| DE3246090C2 (enrdf_load_stackoverflow) | 1991-10-10 |
| IT8224763A0 (it) | 1982-12-15 |
| CA1201047A (en) | 1986-02-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8128 | New person/name/address of the agent |
Representative=s name: JUNG, E., DIPL.-CHEM. DR.PHIL. SCHIRDEWAHN, J., DI |
|
| 8110 | Request for examination paragraph 44 | ||
| 8125 | Change of the main classification |
Ipc: C12Q 1/42 |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: ASAHI KASEI KOGYO K.K., OSAKA, JP |
|
| 8339 | Ceased/non-payment of the annual fee |