CA1201047A - Method for quantitative measurement of phosphatidyl glycerol - Google Patents
Method for quantitative measurement of phosphatidyl glycerolInfo
- Publication number
- CA1201047A CA1201047A CA000416607A CA416607A CA1201047A CA 1201047 A CA1201047 A CA 1201047A CA 000416607 A CA000416607 A CA 000416607A CA 416607 A CA416607 A CA 416607A CA 1201047 A CA1201047 A CA 1201047A
- Authority
- CA
- Canada
- Prior art keywords
- glycerol
- quantitative measurement
- phosphatidyl glycerol
- reaction
- measurement according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000005259 measurement Methods 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 59
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 title claims abstract description 56
- ATBOMIWRCZXYSZ-XZBBILGWSA-N [1-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] (9e,12e)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C\C\C=C\CCCCC ATBOMIWRCZXYSZ-XZBBILGWSA-N 0.000 title claims abstract description 55
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 101
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- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims description 14
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- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002952 polymeric resin Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- HFQQZARZPUDIFP-UHFFFAOYSA-M sodium;2-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HFQQZARZPUDIFP-UHFFFAOYSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- WRPWWVNUCXQDQV-UHFFFAOYSA-N vanillylamine Chemical compound COC1=CC(CN)=CC=C1O WRPWWVNUCXQDQV-UHFFFAOYSA-N 0.000 description 1
- 229940053939 vanillylamine Drugs 0.000 description 1
- PJVWKTKQMONHTI-UHFFFAOYSA-N warfarin Chemical compound OC=1C2=CC=CC=C2OC(=O)C=1C(CC(=O)C)C1=CC=CC=C1 PJVWKTKQMONHTI-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N2405/04—Phospholipids, i.e. phosphoglycerides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP203074/81 | 1981-12-16 | ||
| JP56203074A JPS58107199A (ja) | 1981-12-16 | 1981-12-16 | ホスフアチジルグリセロ−ルの定量方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1201047A true CA1201047A (en) | 1986-02-25 |
Family
ID=16467912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000416607A Expired CA1201047A (en) | 1981-12-16 | 1982-11-29 | Method for quantitative measurement of phosphatidyl glycerol |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS58107199A (enrdf_load_stackoverflow) |
| CA (1) | CA1201047A (enrdf_load_stackoverflow) |
| DE (1) | DE3246090A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2518117B1 (enrdf_load_stackoverflow) |
| GB (1) | GB2111677B (enrdf_load_stackoverflow) |
| IT (1) | IT1155384B (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3211347A1 (de) * | 1982-03-27 | 1983-09-29 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und reagens zur enzymatischen bestimmung von phosphatidylglycerin |
| JPS59140900A (ja) * | 1983-01-28 | 1984-08-13 | Toyo Jozo Co Ltd | 新規な酵素的高感度測定法 |
| JPS62215398A (ja) * | 1986-01-06 | 1987-09-22 | アイソラブ，インク． | 医療診断用指示要素として羊水中のホスフアチジルグリセロ−ルの量を測定する方法とそのキツト |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1515195A (en) * | 1975-08-05 | 1978-06-21 | Hycel Inc | Triglyceride assay |
| JPS604716B2 (ja) * | 1976-04-26 | 1985-02-06 | 東洋醸造株式会社 | 新規なコリンオキシダーゼおよびその製法 |
| JPS5324888A (en) * | 1976-08-19 | 1978-03-08 | Kyowa Hakko Kogyo Kk | Composition for quantitating fats containing phosphorus |
| US4241178A (en) * | 1978-01-06 | 1980-12-23 | Eastman Kodak Company | Process and composition for the quantification of glycerol ATP and triglycerides |
| DK401279A (da) * | 1978-09-26 | 1980-03-27 | Toyo Jozo Kk | Flerkomponentanalysefremgangsmaade samt apparat til anvendelse derved |
| JPS55111800A (en) * | 1979-02-20 | 1980-08-28 | Toyo Jozo Co Ltd | Kit for analysis of lipid component |
| SU831129A1 (ru) * | 1979-07-24 | 1981-05-23 | Дальневосточный Государственный Уни-Верситет | Способ получени фосфатидилглицерина |
| JPS5889199A (ja) * | 1981-11-20 | 1983-05-27 | Toyo Jozo Co Ltd | ホスフアチジルグリセロ−ルの定量法 |
| JPS5898096A (ja) * | 1981-12-07 | 1983-06-10 | Toyo Jozo Co Ltd | エタノ−ルアミン含有液の定量法 |
-
1981
- 1981-12-16 JP JP56203074A patent/JPS58107199A/ja active Pending
-
1982
- 1982-11-23 GB GB08233333A patent/GB2111677B/en not_active Expired
- 1982-11-29 CA CA000416607A patent/CA1201047A/en not_active Expired
- 1982-12-03 FR FR8220269A patent/FR2518117B1/fr not_active Expired
- 1982-12-13 DE DE19823246090 patent/DE3246090A1/de active Granted
- 1982-12-15 IT IT24763/82A patent/IT1155384B/it active
Also Published As
| Publication number | Publication date |
|---|---|
| FR2518117A1 (fr) | 1983-06-17 |
| GB2111677B (en) | 1985-01-16 |
| IT1155384B (it) | 1987-01-28 |
| JPS58107199A (ja) | 1983-06-25 |
| DE3246090A1 (de) | 1984-02-16 |
| FR2518117B1 (fr) | 1986-10-10 |
| IT8224763A1 (it) | 1984-06-15 |
| GB2111677A (en) | 1983-07-06 |
| DE3246090C2 (enrdf_load_stackoverflow) | 1991-10-10 |
| IT8224763A0 (it) | 1982-12-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |