GB1515195A - Triglyceride assay - Google Patents

Triglyceride assay

Info

Publication number
GB1515195A
GB1515195A GB3265775A GB3265775A GB1515195A GB 1515195 A GB1515195 A GB 1515195A GB 3265775 A GB3265775 A GB 3265775A GB 3265775 A GB3265775 A GB 3265775A GB 1515195 A GB1515195 A GB 1515195A
Authority
GB
United Kingdom
Prior art keywords
lipase
triglyceride
units
aqueous liquid
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3265775A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hycel Inc
Original Assignee
Hycel Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hycel Inc filed Critical Hycel Inc
Priority to GB3265775A priority Critical patent/GB1515195A/en
Priority to FR7623667A priority patent/FR2320552A1/en
Priority to DE19762635040 priority patent/DE2635040C2/en
Priority to IT2601176A priority patent/IT1065245B/en
Priority to ES450915A priority patent/ES450915A1/en
Priority to JP9352676A priority patent/JPS5225693A/en
Priority to JP9352776A priority patent/JPS5225694A/en
Publication of GB1515195A publication Critical patent/GB1515195A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1515195 Determination of triglyceride HYCEL Inc 23 July 1976 [5 Aug 1975] 32657/75 Heading G1B [Also in Division C3] The content of triglyceride is assayed in samples containing it, e.g. blood serum by treating the sample with a reagent comprising a lipase which consists of Rhizopus Arrhizus lipase and Candida Cylindracea lipase thereby converting the triglycerides to glycerol and fatty acids which can subsequently be determined. Preferably 16-40 units of Candida Cylindracea lipase and 30-50 units of Rhizopus Arrhizus lipase are provided for the assay of each 0À05 mls sample of said aqueous liquid. The aqueous liquid and lipase may be incubated at 37‹C for 10 minutes.
GB3265775A 1975-08-05 1975-08-05 Triglyceride assay Expired GB1515195A (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
GB3265775A GB1515195A (en) 1975-08-05 1975-08-05 Triglyceride assay
FR7623667A FR2320552A1 (en) 1975-08-05 1976-08-03 METHOD OF DETERMINING THE CONTENT OF AN AQUEOUS GLYCEROLE FATTY ESTER SOLUTION
DE19762635040 DE2635040C2 (en) 1975-08-05 1976-08-04 Reagent for the hydrolysis of glycerol fatty acid esters, as well as a method for the analysis of an aqueous liquid with a content of glycerol fatty acid esters
IT2601176A IT1065245B (en) 1975-08-05 1976-08-04 REAGENT FOR THE ANALYSIS OF TRIGLI CERIDI AND METHOD FOR ITS USE
ES450915A ES450915A1 (en) 1975-08-05 1976-08-05 Triglyceride assay
JP9352676A JPS5225693A (en) 1975-08-05 1976-08-05 Reagent for analyzing aliphatic glycerin ester and method of analysis
JP9352776A JPS5225694A (en) 1975-08-05 1976-08-05 Reagent for analyzing aliphatic glycerin ester and method of analysis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3265775A GB1515195A (en) 1975-08-05 1975-08-05 Triglyceride assay

Publications (1)

Publication Number Publication Date
GB1515195A true GB1515195A (en) 1978-06-21

Family

ID=10342034

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3265775A Expired GB1515195A (en) 1975-08-05 1975-08-05 Triglyceride assay

Country Status (6)

Country Link
JP (2) JPS5225693A (en)
DE (1) DE2635040C2 (en)
ES (1) ES450915A1 (en)
FR (1) FR2320552A1 (en)
GB (1) GB1515195A (en)
IT (1) IT1065245B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1148142A1 (en) * 1999-01-20 2001-10-24 Kyowa Medex Co., Ltd. Method for quantitating triglyceride in lipoprotein

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4338395A (en) * 1980-07-21 1982-07-06 Technicon Instruments Corporation Method for the analysis of triglycerides
JPS58107199A (en) * 1981-12-16 1983-06-25 Toyo Jozo Co Ltd Qualitative analysis of phosphatidyl glycerol

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2000127C3 (en) * 1970-01-02 1974-12-12 Boehringer Mannheim Gmbh Method for the quantitative cleavage and for the quantitative detection of tri-, di- and monoglycerides
FR2212046A5 (en) * 1972-12-25 1974-07-19 Ono Pharmaceutical Co
DE2315501C3 (en) * 1973-03-28 1980-02-21 Boehringer Mannheim Gmbh, 6800 Mannheim Method for the determination of cholesterol

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1148142A1 (en) * 1999-01-20 2001-10-24 Kyowa Medex Co., Ltd. Method for quantitating triglyceride in lipoprotein
EP1148142A4 (en) * 1999-01-20 2005-07-27 Kyowa Medex Co Ltd Method for quantitating triglyceride in lipoprotein

Also Published As

Publication number Publication date
IT1065245B (en) 1985-02-25
DE2635040A1 (en) 1977-02-17
DE2635040C2 (en) 1984-06-14
FR2320552B1 (en) 1981-05-29
ES450915A1 (en) 1977-08-16
JPS5225694A (en) 1977-02-25
FR2320552A1 (en) 1977-03-04
JPS5225693A (en) 1977-02-25

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Legal Events

Date Code Title Description
PS Patent sealed
PE20 Patent expired after termination of 20 years

Effective date: 19960722