FR2449726A1 - Fast reacting lipase compsn. for hydrolysis of glycerol ester(s) - contg. Rhizopus arrhizus lipase and Pseudomonas Fluoresens lipase - Google Patents

Fast reacting lipase compsn. for hydrolysis of glycerol ester(s) - contg. Rhizopus arrhizus lipase and Pseudomonas Fluoresens lipase

Info

Publication number
FR2449726A1
FR2449726A1 FR8003817A FR8003817A FR2449726A1 FR 2449726 A1 FR2449726 A1 FR 2449726A1 FR 8003817 A FR8003817 A FR 8003817A FR 8003817 A FR8003817 A FR 8003817A FR 2449726 A1 FR2449726 A1 FR 2449726A1
Authority
FR
France
Prior art keywords
lipase
hydrolysis
glycerol
compsn
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR8003817A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Millipore Corp filed Critical Millipore Corp
Publication of FR2449726A1 publication Critical patent/FR2449726A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

New enzyme compsns. for the hydrolysis of glycerol esters in aq. media comprise (A) ca 65-91 units of lipase from Rhizopus arrhizus and (B) ca 35-9 units of lipase from Pseudomonas fluorescens, per 100 units of total lipase present in the compsn. New reagent compsns. for determn. of the concn. of triglycerides in serum by hydrolysis of the triglycerides to glycerol and free fatty acids and measurement of the light absorption of a glycerol-contg. aq. liq. resulting from the hydrolysis comprise an enzyme mixt. as defined above, together with chemical reagents to bring about a change in the absorption of the aq. liq. which is proportional to the amt. of glycerol released in the serum. New process for the hydrolysis of glycerol esters and for the determn. of the amt. of glycerol esters present in aq. body fluids comprises incubating the fluid under investigation with an enzyme compsn. or reagent compsn. as defined above for ca 10 mins. to effect complete hydrolysis of the glycerol esters, and determn. of the hydrolytically released amt. of glycerol and/or fatty acids.
FR8003817A 1979-02-22 1980-02-21 Fast reacting lipase compsn. for hydrolysis of glycerol ester(s) - contg. Rhizopus arrhizus lipase and Pseudomonas Fluoresens lipase Withdrawn FR2449726A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1386279A 1979-02-22 1979-02-22

Publications (1)

Publication Number Publication Date
FR2449726A1 true FR2449726A1 (en) 1980-09-19

Family

ID=21762173

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8003817A Withdrawn FR2449726A1 (en) 1979-02-22 1980-02-21 Fast reacting lipase compsn. for hydrolysis of glycerol ester(s) - contg. Rhizopus arrhizus lipase and Pseudomonas Fluoresens lipase

Country Status (3)

Country Link
JP (1) JPS55159794A (en)
DE (1) DE3006789A1 (en)
FR (1) FR2449726A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2536416A1 (en) * 1982-11-19 1984-05-25 Toyo Jozo Kk COMPOSITION FOR THE DETERMINATION OF LIPASES

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5754599A (en) * 1980-07-22 1982-04-01 Baker Instr Corp Improved glycerol detecting reagent
JPS5784420A (en) * 1981-06-23 1982-05-26 Olympus Optical Co Ltd Endoscope
JPS5784421A (en) * 1981-06-23 1982-05-26 Olympus Optical Co Ltd Endoscope
JPS5977268A (en) * 1982-10-25 1984-05-02 株式会社東芝 Air conditioner
JPS6030680A (en) * 1983-07-30 1985-02-16 Amano Pharmaceut Co Ltd Method for imparting esterase activity to lipase
JPS6136659A (en) * 1984-07-27 1986-02-21 株式会社日立製作所 Heat pump type air conditioner
JPS6146831A (en) * 1984-08-10 1986-03-07 Matsushita Electric Ind Co Ltd Defrosting operation control device in air conditioning machine
JP4394598B2 (en) * 2004-08-24 2010-01-06 日清オイリオグループ株式会社 Lipase powder composition and method for producing esterified product using the same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2190277A5 (en) * 1972-06-19 1974-01-25 Boehringer Mannheim Gmbh
FR2212046A5 (en) * 1972-12-25 1974-07-19 Ono Pharmaceutical Co
FR2353859A1 (en) * 1976-06-01 1977-12-30 Dow Chemical Co COMPOSITION AND PROCESS FOR THE HYDROLYSIS OF GLYCERINE ESTERS WITH A VIEW TO THEIR DOSAGE
WO1980000260A1 (en) * 1978-07-13 1980-02-21 American Hospital Supply Corp Determination of triglycerides and enzyme reagents

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3431175A (en) * 1964-07-18 1969-03-04 Amano Pharma Co Ltd Method for the preparation of a lipoprotein lipase by cultivating organisms
US3513073A (en) * 1968-07-01 1970-05-19 Roland Yves Mauvernay Novel lipase composition and method for producing same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2190277A5 (en) * 1972-06-19 1974-01-25 Boehringer Mannheim Gmbh
FR2212046A5 (en) * 1972-12-25 1974-07-19 Ono Pharmaceutical Co
FR2353859A1 (en) * 1976-06-01 1977-12-30 Dow Chemical Co COMPOSITION AND PROCESS FOR THE HYDROLYSIS OF GLYCERINE ESTERS WITH A VIEW TO THEIR DOSAGE
WO1980000260A1 (en) * 1978-07-13 1980-02-21 American Hospital Supply Corp Determination of triglycerides and enzyme reagents

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2536416A1 (en) * 1982-11-19 1984-05-25 Toyo Jozo Kk COMPOSITION FOR THE DETERMINATION OF LIPASES

Also Published As

Publication number Publication date
DE3006789A1 (en) 1980-09-04
JPS55159794A (en) 1980-12-12
JPS5728276B2 (en) 1982-06-15

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ST Notification of lapse