DE69024364T2 - Verstärkter chemilumineszenzassay - Google Patents

Verstärkter chemilumineszenzassay

Info

Publication number
DE69024364T2
DE69024364T2 DE69024364T DE69024364T DE69024364T2 DE 69024364 T2 DE69024364 T2 DE 69024364T2 DE 69024364 T DE69024364 T DE 69024364T DE 69024364 T DE69024364 T DE 69024364T DE 69024364 T2 DE69024364 T2 DE 69024364T2
Authority
DE
Germany
Prior art keywords
reinforced
chemiluminescence assay
chemiluminescence
assay
reinforced chemiluminescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69024364T
Other languages
English (en)
Other versions
DE69024364D1 (de
Inventor
Larry Kricka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BTG International Ltd
Original Assignee
British Technology Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB898923344A external-priority patent/GB8923344D0/en
Priority claimed from GB909010763A external-priority patent/GB9010763D0/en
Application filed by British Technology Group Ltd filed Critical British Technology Group Ltd
Application granted granted Critical
Publication of DE69024364D1 publication Critical patent/DE69024364D1/de
Publication of DE69024364T2 publication Critical patent/DE69024364T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives
DE69024364T 1989-10-17 1990-10-16 Verstärkter chemilumineszenzassay Expired - Fee Related DE69024364T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB898923344A GB8923344D0 (en) 1989-10-17 1989-10-17 Enhanced chemiluminescent assay
GB909010763A GB9010763D0 (en) 1990-05-14 1990-05-14 Enhanced chemiluminescent assay(2)
PCT/GB1990/001597 WO1991005872A1 (en) 1989-10-17 1990-10-16 Enhanced chemiluminescent assay

Publications (2)

Publication Number Publication Date
DE69024364D1 DE69024364D1 (de) 1996-02-01
DE69024364T2 true DE69024364T2 (de) 1996-07-11

Family

ID=26296061

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69024364T Expired - Fee Related DE69024364T2 (de) 1989-10-17 1990-10-16 Verstärkter chemilumineszenzassay

Country Status (5)

Country Link
US (1) US5306621A (de)
EP (1) EP0496793B1 (de)
JP (1) JP2977895B2 (de)
DE (1) DE69024364T2 (de)
WO (1) WO1991005872A1 (de)

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US5856522A (en) * 1995-05-04 1999-01-05 Tropix, Inc. Method of using synthesis of 1,2-dioxetanes and kits therefore
CA2061189A1 (en) * 1991-05-24 1992-11-25 M. Hashem Akhavan-Tafti Chemiluminescent method and compositions
US5629168A (en) * 1992-02-10 1997-05-13 British Technology Group Limited Chemiluminescent enhancers
DE69318966T2 (de) * 1992-02-10 1998-11-12 British Tech Group Chemilumineszenzverstärker
US5686258A (en) * 1993-05-17 1997-11-11 Lumigen, Inc. Chemiluminescent detection of hydrolytic enzymes using an acridan
US5843666A (en) * 1994-09-02 1998-12-01 Lumigen, Inc. Chemiluminescent detection methods using dual enzyer-labeled binding partners
US5801008A (en) * 1995-05-31 1998-09-01 Kyowa Medex Co., Ltd. Method of quantitative determination of peroxide, a peroxidation-active substance or a pyrazolopyridopyridazine derivative
US5872010A (en) * 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US5811253A (en) * 1995-09-01 1998-09-22 Johnson & Johnson Clinical Diagnostics, Inc. Use of vanadium bromoperoxidase as a signal-generating enzyme for chemiluminescent systems: test kits and analytical methods
US6602657B1 (en) 1995-12-28 2003-08-05 Tropix, Inc. Multiple reporter gene assay
JP3169383B2 (ja) * 1996-01-16 2001-05-21 ルミゲン インク ホスファターゼ酵素で化学発光を生成させるための化合物、組成物および方法
US5772926A (en) * 1997-05-13 1998-06-30 Lumigen, Inc. Chemiluminescent reactions using dihydroxyaromatic compounds and heterocyclic enol phosphates
DE19748489A1 (de) * 1997-11-03 1999-05-06 Roche Diagnostics Gmbh Polyethylenglykol-derivatisierte Biomoleküle und deren Verwendung in heterogenen Nachweisverfahren
US6245227B1 (en) 1998-09-17 2001-06-12 Kionix, Inc. Integrated monolithic microfabricated electrospray and liquid chromatography system and method
CN100435900C (zh) * 1998-09-17 2008-11-26 阿德文生物科学公司 液相色谱系统，化学分离装置及质谱分析装置和方法
US6518036B1 (en) * 1999-03-17 2003-02-11 Nen Life Science Products, Inc. Method of permanent fluorescent assay
US6633031B1 (en) 1999-03-02 2003-10-14 Advion Biosciences, Inc. Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method
US6783948B1 (en) * 1999-07-30 2004-08-31 Bayer Corporation Chemiluminescent acridinium compounds and analogues thereof as substrates of hydrolytic enzymes
AU7839500A (en) * 1999-10-01 2001-05-10 Applied Imaging Corporation Simultaneous enzymatic detection of two analytes
JP5057318B2 (ja) 1999-12-30 2012-10-24 アドビオン インコーポレイテッド 多重電気噴霧装置、システム、および方法
EP1248949B1 (de) 2000-01-18 2013-05-22 Advion, Inc. Elektrosprayvorrichtung mit array von trennsäulen sowie verfahren zur trennung von fluidproben
WO2002033396A1 (en) * 2000-10-14 2002-04-25 Saunders Alex M Ligand based solution assay for low concentration analytes
US20050233473A1 (en) * 2002-08-16 2005-10-20 Zyomyx, Inc. Methods and reagents for surface functionalization
US20050272108A1 (en) * 2004-05-21 2005-12-08 Bhanu Kalra Stabilized two component system for chemiluminescent assay in immunodiagnostics
WO2006116687A2 (en) * 2005-04-27 2006-11-02 The Trustees Of The University Of Pennsylvania Nanoassays
WO2006116686A2 (en) * 2005-04-27 2006-11-02 The Trustees Of The University Of Pennsylvania Nanostructure enhanced luminescent devices
TW200808974A (en) * 2006-08-02 2008-02-16 Ind Tech Res Inst Luminescence-based recipe
CN101657150B (zh) 2007-01-26 2013-04-03 弗洛西恩公司 用于分析系统的传感器
WO2008089766A1 (en) 2007-01-26 2008-07-31 Diramo A/S Analysis system with a remote analysing unit
US7674629B2 (en) * 2007-03-15 2010-03-09 Saint Louis University Method for improving chemiluminescent signal
US20090081717A1 (en) * 2007-09-18 2009-03-26 Mahant Vijay K Compositions and Methods For Signal Generation
WO2009137666A2 (en) * 2008-05-08 2009-11-12 The Trustees Of The University Of Pennsylvania Chemiluminescence enhanced detection
US20110097723A1 (en) * 2009-09-19 2011-04-28 Qun Liu Methods and reagents for analyte detection
EP2524206A1 (de) 2010-01-13 2012-11-21 Flowsion ApS Mikrofluidisches system mit einer lichtemittierenden vorrichtung
WO2012062320A1 (en) 2010-11-10 2012-05-18 Flowsion A/S Micro -fluid analysis system with pressurised waste reservoir

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4307188A (en) * 1979-09-06 1981-12-22 Miles Laboratories, Inc. Precursor indicator compositions
WO1981000725A1 (en) * 1979-09-14 1981-03-19 Charles Hospital Dev Method of determining a substrate in a sample
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
EP0049606A1 (de) * 1980-10-02 1982-04-14 Colin Henry Self Nachweisverfahren, Anwendung und diagnostisches Hilfsmittel
US4842997A (en) * 1982-03-03 1989-06-27 National Research Development Corporation Enhanced luminescent and luminometric assay
US4477578A (en) * 1982-03-04 1984-10-16 Medical & Scientific, Inc. Method and apparatus for performing assays
EP0116454B1 (de) * 1983-02-11 1987-04-29 National Research Development Corporation Erhöhte lumineszierende oder luminometrische Prüfung
GB8407736D0 (en) * 1984-03-26 1984-05-02 Univ London Detecting specific polynucleotide sequences
GB8420053D0 (en) * 1984-08-07 1984-09-12 Secr Social Service Brit Enhanced luminescent/luminometric assay
DE3537877A1 (de) * 1985-10-24 1987-04-30 Geiger Reinhard Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate
GB8713951D0 (en) * 1987-06-15 1987-07-22 Dewar M H Enhanced chemiluminescent reaction
JPH07114709B2 (ja) * 1987-11-13 1995-12-13 協和メデックス株式会社 酵素活性の定量法

Also Published As

Publication number Publication date
EP0496793A1 (de) 1992-08-05
WO1991005872A1 (en) 1991-05-02
EP0496793B1 (de) 1995-12-20
JP2977895B2 (ja) 1999-11-15
JPH05501203A (ja) 1993-03-11
US5306621A (en) 1994-04-26
DE69024364D1 (de) 1996-02-01

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: BTG INTERNATIONAL LTD., LONDON, GB

8339 Ceased/non-payment of the annual fee