DE2539242A1 - Radioimmunologisches analyseverfahren zur bestimmung von digoxin - Google Patents
Radioimmunologisches analyseverfahren zur bestimmung von digoxinInfo
- Publication number
- DE2539242A1 DE2539242A1 DE19752539242 DE2539242A DE2539242A1 DE 2539242 A1 DE2539242 A1 DE 2539242A1 DE 19752539242 DE19752539242 DE 19752539242 DE 2539242 A DE2539242 A DE 2539242A DE 2539242 A1 DE2539242 A1 DE 2539242A1
- Authority
- DE
- Germany
- Prior art keywords
- digoxin
- digoxigenin
- sample
- mixture
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229960005156 digoxin Drugs 0.000 title claims description 69
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 title claims description 66
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 title claims description 66
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 title claims description 57
- 238000004458 analytical method Methods 0.000 title description 16
- 210000002966 serum Anatomy 0.000 claims description 23
- -1 4-hydroxyphenethylcarbamoyl Chemical group 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 13
- 238000011088 calibration curve Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 239000002202 Polyethylene glycol Substances 0.000 claims description 9
- 229920001223 polyethylene glycol Polymers 0.000 claims description 9
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims description 8
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims description 8
- 239000002244 precipitate Substances 0.000 claims description 8
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims description 7
- 239000000700 radioactive tracer Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003708 ampul Substances 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 238000002405 diagnostic procedure Methods 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 239000000427 antigen Substances 0.000 description 17
- 108091007433 antigens Proteins 0.000 description 17
- 102000036639 antigens Human genes 0.000 description 17
- 238000012360 testing method Methods 0.000 description 15
- 239000000243 solution Substances 0.000 description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 230000002285 radioactive effect Effects 0.000 description 5
- 239000003755 preservative agent Substances 0.000 description 4
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 235000019341 magnesium sulphate Nutrition 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 239000007982 barbital buffer Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000921 elemental analysis Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- YGYAWVDWMABLBF-UHFFFAOYSA-N Phosgene Chemical compound ClC(Cl)=O YGYAWVDWMABLBF-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 description 1
- WFKAJVHLWXSISD-UHFFFAOYSA-N anhydrous dimethyl-acetamide Natural products CC(C)C(N)=O WFKAJVHLWXSISD-UHFFFAOYSA-N 0.000 description 1
- 238000010533 azeotropic distillation Methods 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- HCWCAKKEBCNQJP-UHFFFAOYSA-N magnesium orthosilicate Chemical compound [Mg+2].[Mg+2].[O-][Si]([O-])([O-])[O-] HCWCAKKEBCNQJP-UHFFFAOYSA-N 0.000 description 1
- 239000000391 magnesium silicate Substances 0.000 description 1
- 229910052919 magnesium silicate Inorganic materials 0.000 description 1
- 235000019792 magnesium silicate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000012746 preparative thin layer chromatography Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000003345 scintillation counting Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- 229960003732 tyramine Drugs 0.000 description 1
- DZGWFCGJZKJUFP-UHFFFAOYSA-O tyraminium Chemical compound [NH3+]CCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-O 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
- C07J41/0033—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005
- C07J41/0055—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005 the 17-beta position being substituted by an uninterrupted chain of at least three carbon atoms which may or may not be branched, e.g. cholane or cholestane derivatives, optionally cyclised, e.g. 17-beta-phenyl or 17-beta-furyl derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J19/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 by a lactone ring
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9453—Cardioregulators, e.g. antihypotensives, antiarrhythmics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/804—Radioisotope, e.g. radioimmunoassay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Heart & Thoracic Surgery (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cardiology (AREA)
- Steroid Compounds (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/505,185 US3981982A (en) | 1974-09-11 | 1974-09-11 | Radioimmunoassay for determining the digoxin content of a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2539242A1 true DE2539242A1 (de) | 1976-04-01 |
Family
ID=24009363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752539242 Pending DE2539242A1 (de) | 1974-09-11 | 1975-09-03 | Radioimmunologisches analyseverfahren zur bestimmung von digoxin |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US3981982A (https=) |
| CA (1) | CA1036592A (https=) |
| DE (1) | DE2539242A1 (https=) |
| FR (1) | FR2285613A1 (https=) |
| GB (2) | GB1508018A (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5315359A (en) * | 1976-05-17 | 1978-02-13 | Union Carbide Corp | Novel isocyanate composition |
| EP0044041A1 (de) * | 1980-07-14 | 1982-01-20 | Hoechst Aktiengesellschaft | Verfahren zum Binden und Trennen für den kompetitiven Radioimmunoassay und Reagenz hierzu |
| EP0044988A3 (en) * | 1980-07-30 | 1982-05-05 | Veb Arzneimittelwerk Dresden | Separation of the free and antibody-bound ligand for labelled-ligand immuno-assays |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4255329A (en) * | 1973-10-02 | 1981-03-10 | Syva Company | Double receptor fluorescent immunoassay |
| GB1553222A (en) * | 1975-05-07 | 1979-09-26 | Steele Chemical Co Ltd | 14 - hydroxy 3- eoxycardenolides |
| DE2537129C3 (de) | 1975-08-20 | 1978-05-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | Reaktive unsymmetrische, einen Digoxin- bzw. Digitoxinrest enthaltende Dicarbonsäureester, Verfahren zu deren Herstellung sowie ihre Verwendung zur Herstellung von Testreagentien |
| US4181650A (en) * | 1975-08-25 | 1980-01-01 | Maier Charles L Jr | Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids |
| US4221725A (en) * | 1977-08-12 | 1980-09-09 | E. R. Squibb & Sons, Inc. | Steroid derivatives and their use in radioimmunoassays |
| US4184849A (en) * | 1977-12-05 | 1980-01-22 | Technicon Instruments Corporation | Mixed agglutination |
| US4345096A (en) * | 1979-01-17 | 1982-08-17 | E. R. Squibb & Sons, Inc. | Steroid derivatives and their use in radioimmunoassays |
| WO1983004102A1 (en) * | 1982-05-07 | 1983-11-24 | Paragon Diagnostics | Competitive immunofluorescence assay and test kit |
| JPS59500832A (ja) * | 1982-05-07 | 1984-05-10 | パラゴン・ダイアグノスティクス・インコ−ポレイテッド | 競合的免疫螢光検定法及び試験セット |
| US4654311A (en) * | 1984-06-15 | 1987-03-31 | Syntex (U.S.A.) Inc. | Serum pretreatment for digoxin assay |
| EP0933355A1 (en) * | 1997-12-24 | 1999-08-04 | Universiteit Maastricht | Preparation of tyramide conjugates |
| ES2857626T3 (es) * | 2010-01-15 | 2021-09-29 | Suzhou Neupharma Co Ltd | Ciertas entidades químicas, composiciones y métodos |
| CN102656179B (zh) | 2010-08-28 | 2015-07-29 | 苏州润新生物科技有限公司 | 蟾蜍灵衍生物、其药物组合物及用途 |
| US9493503B2 (en) * | 2011-02-02 | 2016-11-15 | Neupharma, Inc. | Certain chemical entities, compositions, and methods |
| WO2013165924A1 (en) | 2012-04-29 | 2013-11-07 | Neupharma, Inc. | Certain chemical entities, compositions, and methods |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH459997A (de) * | 1963-12-05 | 1968-07-31 | Shalom Prof Sarel | Verfahren zur Herstellung von Cardenoliden |
-
1974
- 1974-09-11 US US05/505,185 patent/US3981982A/en not_active Expired - Lifetime
-
1975
- 1975-08-26 GB GB35117/75A patent/GB1508018A/en not_active Expired
- 1975-08-26 GB GB20309/77A patent/GB1508019A/en not_active Expired
- 1975-08-26 CA CA234,197A patent/CA1036592A/en not_active Expired
- 1975-09-03 DE DE19752539242 patent/DE2539242A1/de active Pending
- 1975-09-10 FR FR7527794A patent/FR2285613A1/fr active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5315359A (en) * | 1976-05-17 | 1978-02-13 | Union Carbide Corp | Novel isocyanate composition |
| EP0044041A1 (de) * | 1980-07-14 | 1982-01-20 | Hoechst Aktiengesellschaft | Verfahren zum Binden und Trennen für den kompetitiven Radioimmunoassay und Reagenz hierzu |
| EP0044988A3 (en) * | 1980-07-30 | 1982-05-05 | Veb Arzneimittelwerk Dresden | Separation of the free and antibody-bound ligand for labelled-ligand immuno-assays |
Also Published As
| Publication number | Publication date |
|---|---|
| GB1508018A (en) | 1978-04-19 |
| US3981982A (en) | 1976-09-21 |
| CA1036592A (en) | 1978-08-15 |
| FR2285613A1 (fr) | 1976-04-16 |
| AU8430775A (en) | 1977-03-03 |
| GB1508019A (en) | 1978-04-19 |
| FR2285613B1 (https=) | 1981-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE2539242A1 (de) | Radioimmunologisches analyseverfahren zur bestimmung von digoxin | |
| DE2936307C2 (https=) | ||
| DE2600465C3 (de) | Verfahren zur radioimmunologischen Bestimmung von Östrogenen | |
| DE2734118A1 (de) | Radioimmunologisches verfahren und einrichtung zur bestimmung von antigenen | |
| DE2262413A1 (de) | Direkte radioimmunpruefung auf antigene und ihre antikoerper | |
| DE4120412C1 (https=) | ||
| EP0035211B1 (de) | Verfahren und Reagens zur Bestimmung der beta-Lipoproteine (LDL) | |
| DE2538388C3 (de) | Verfahren zur Herstellung eines stabilen, nicht-radioaktiven Trägermaterials für mit radioaktiven Elementen markierte Diagnosemittel und seine Verwendung für mit Tc-99m-markierte Diagnosemittel | |
| DE69223851T2 (de) | Nitro- oder Nitroso-substituierte polyhalogenierte Phenolsulfonphtaleine | |
| CH627188A5 (https=) | ||
| DE2607826A1 (de) | Verfahren zur radioimmunologischen in-vitro-bestimmung von kardiotenischen glykosiden und abgepacktes testbesteck zur durchfuehrung dieses verfahrens | |
| DE2722038A1 (de) | Stoffzusammensetzungen auf basis von isocyanat-reaktrionsprodukten, verfahren zu ihrer herstellung und ihre verwendung | |
| DE3877032T2 (de) | Diagnostischer immunoassay fuer digoxin mit festphasetrennung. | |
| DE3650691T2 (de) | Verfahren zum Messen von Freitestosteronen in biologischen Flüssigkeiten | |
| DE2600835A1 (de) | Digoxinderivate, verfahren zu ihrer herstellung und ihre verwendung zur radioimmunbestimmung von digoxin | |
| EP0303284B2 (de) | Immunologisches Bestimmungsverfahren zur Bestimmung von Hapteneigenschaften aufweisenden freien Substanzen | |
| DE2916783C3 (de) | Verfahren zur immunologischen Bestimmung einer Gallensäure oder ihres Konjugats | |
| DE2537275C3 (de) | Immunchemisches Verfahren zur Bestimmung von Antigenen oder Antikörpern in biologischen Flüssigkeiten | |
| EP0190765B1 (de) | Verfahren zur Bestimmung der Bindungskapazität des Thyroxin bindenden Globulins | |
| DE2743446B2 (de) | Histaminderivate des Digoxins und ihre Verwendung zum Messen des Digoxingehaltes einer Serumprobe | |
| DE68906742T2 (de) | Testsatz zum Nachweis von denaturierten Lipoproteinen. | |
| DE2431255C3 (de) | Amino-alkyläther des Morphins, Verfahren zu ihrer Herstellung und ihre Verwendung zur Herstellung eines Reagens zur antikörperspezifischen Bestimmung von Opiumalkaloiden | |
| DE2526984C3 (de) | Digoxin- und Digitoxinderivate, Verfahren zu ihrer Herstellung und ihre Verwendung zur Bestimmung von Digoxin und Digitoxin | |
| DE2720809A1 (de) | Radioimmunoassay-reagens und dessen verwendung in einem radioimmunoassay- verfahren | |
| DE2814776C3 (de) | Neue östradiolderivate und diagnostisches Mittel |