DE2506712C3 - Verfahren zur Bestimmung von Cholesterin - Google Patents
Verfahren zur Bestimmung von CholesterinInfo
- Publication number
- DE2506712C3 DE2506712C3 DE19752506712 DE2506712A DE2506712C3 DE 2506712 C3 DE2506712 C3 DE 2506712C3 DE 19752506712 DE19752506712 DE 19752506712 DE 2506712 A DE2506712 A DE 2506712A DE 2506712 C3 DE2506712 C3 DE 2506712C3
- Authority
- DE
- Germany
- Prior art keywords
- cholesterol
- determination
- esterase
- bound
- streptomyces
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title description 114
- 235000012000 cholesterol Nutrition 0.000 title description 57
- 238000000034 method Methods 0.000 title description 31
- 108010055297 Sterol Esterase Proteins 0.000 description 25
- 102000000019 Sterol Esterase Human genes 0.000 description 25
- 244000005700 microbiome Species 0.000 description 20
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 150000001840 cholesterol esters Chemical class 0.000 description 7
- 230000002255 enzymatic effect Effects 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 241000222175 Diutina rugosa Species 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 241000228212 Aspergillus Species 0.000 description 4
- 241000235527 Rhizopus Species 0.000 description 4
- 241000187747 Streptomyces Species 0.000 description 4
- 238000007127 saponification reaction Methods 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 241000222122 Candida albicans Species 0.000 description 3
- 108010089254 Cholesterol oxidase Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 229940095731 candida albicans Drugs 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000589220 Acetobacter Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241000187654 Nocardia Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 241000520747 Streptomyces canescens Species 0.000 description 2
- 241000936713 Streptomyces griseomycini Species 0.000 description 2
- 241000187234 Streptomyces longisporus Species 0.000 description 2
- 241000946855 Streptomyces malachiticus Species 0.000 description 2
- 241001468239 Streptomyces murinus Species 0.000 description 2
- 241000946767 Streptomyces toxytricini Species 0.000 description 2
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000001166 ammonium sulphate Substances 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 108010023417 cholesterol dehydrogenase Proteins 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 108010085346 steroid delta-isomerase Proteins 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 101100072420 Caenorhabditis elegans ins-5 gene Proteins 0.000 description 1
- 229920000049 Carbon (fiber) Polymers 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241000235556 Cunninghamella elegans Species 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 241001149951 Mucor mucedo Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 241000204087 Pseudonocardia autotrophica Species 0.000 description 1
- 241000187563 Rhodococcus ruber Species 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 241000187130 Streptomyces chartreusis Species 0.000 description 1
- 241000936751 Streptomyces cyaneofuscatus Species 0.000 description 1
- 241000936729 Streptomyces hachijoensis Species 0.000 description 1
- 241000946864 Streptomyces michiganensis Species 0.000 description 1
- 241000946794 Streptomyces roseolus Species 0.000 description 1
- 241000187179 Streptomyces tendae Species 0.000 description 1
- 241000946738 Streptomyces variabilis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004917 carbon fiber Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- 125000004185 ester group Chemical group 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229940044734 hydase Drugs 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT167974A AT335626B (de) | 1974-03-01 | 1974-03-01 | Verfahren und reagens zur bestimmung von gesamtcholesterin oder gebundenem cholesterin |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2506712A1 DE2506712A1 (de) | 1975-09-04 |
| DE2506712B2 DE2506712B2 (de) | 1979-08-30 |
| DE2506712C3 true DE2506712C3 (de) | 1980-05-14 |
Family
ID=3518642
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752506712 Expired DE2506712C3 (de) | 1974-03-01 | 1975-02-18 | Verfahren zur Bestimmung von Cholesterin |
| DE19752559875 Granted DE2559875B1 (de) | 1974-03-01 | 1975-02-18 | Verfahren zur Bestimmung von Cholesterin |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752559875 Granted DE2559875B1 (de) | 1974-03-01 | 1975-02-18 | Verfahren zur Bestimmung von Cholesterin |
Country Status (2)
| Country | Link |
|---|---|
| AT (1) | AT335626B (enExample) |
| DE (2) | DE2506712C3 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2512585A1 (de) * | 1974-03-25 | 1975-10-02 | Eastman Kodak Co | Verfahren zur bestimmung des gesamt-cholesteringehaltes sowie testloesung und analytisches element zur durchfuehrung des verfahrens |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3032377A1 (de) * | 1980-08-28 | 1982-04-01 | Battelle-Institut E.V., 6000 Frankfurt | Verfahren zur bestimmung von gesamtcholesterin |
| DE4018152A1 (de) * | 1990-06-06 | 1991-12-12 | Boehringer Mannheim Gmbh | Cholesterinesterasen mit variabler substrat-spezifitaet |
-
1974
- 1974-03-01 AT AT167974A patent/AT335626B/de not_active IP Right Cessation
-
1975
- 1975-02-18 DE DE19752506712 patent/DE2506712C3/de not_active Expired
- 1975-02-18 DE DE19752559875 patent/DE2559875B1/de active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2512585A1 (de) * | 1974-03-25 | 1975-10-02 | Eastman Kodak Co | Verfahren zur bestimmung des gesamt-cholesteringehaltes sowie testloesung und analytisches element zur durchfuehrung des verfahrens |
| DE2512585C3 (de) * | 1974-03-25 | 1986-07-31 | Eastman Kodak Co., Rochester, N.Y. | Verfahren, Testlösung und analytisches Element für die Bestimmung des Gesamt-Cholesteringehaltes von Cholesterin und Cholesterinester enthaltenden Mischungen |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2506712B2 (de) | 1979-08-30 |
| DE2559875B1 (de) | 1979-10-25 |
| AT335626B (de) | 1977-03-25 |
| ATA167974A (de) | 1976-07-15 |
| DE2506712A1 (de) | 1975-09-04 |
| DE2559875C2 (enExample) | 1980-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OI | Miscellaneous see part 1 | ||
| OI | Miscellaneous see part 1 | ||
| C3 | Grant after two publication steps (3rd publication) | ||
| AG | Has addition no. |
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