DE1767899A1 - Verfahren zur Isolierung von Antigenen und Antikoerpern - Google Patents
Verfahren zur Isolierung von Antigenen und AntikoerpernInfo
- Publication number
- DE1767899A1 DE1767899A1 DE19681767899 DE1767899A DE1767899A1 DE 1767899 A1 DE1767899 A1 DE 1767899A1 DE 19681767899 DE19681767899 DE 19681767899 DE 1767899 A DE1767899 A DE 1767899A DE 1767899 A1 DE1767899 A1 DE 1767899A1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- solution
- antibodies
- antigens
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000036639 antigens Human genes 0.000 title claims description 33
- 108091007433 antigens Proteins 0.000 title claims description 33
- 239000000427 antigen Substances 0.000 title claims description 30
- 238000000034 method Methods 0.000 title claims description 22
- 238000002955 isolation Methods 0.000 title claims description 7
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 42
- 102000004169 proteins and genes Human genes 0.000 claims description 38
- 108090000623 proteins and genes Proteins 0.000 claims description 38
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 31
- 229920000642 polymer Polymers 0.000 claims description 24
- 235000019260 propionic acid Nutrition 0.000 claims description 21
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims description 21
- 238000010828 elution Methods 0.000 claims description 19
- 238000001556 precipitation Methods 0.000 claims description 16
- 239000002156 adsorbate Substances 0.000 claims description 7
- 238000001179 sorption measurement Methods 0.000 claims description 7
- 239000012736 aqueous medium Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000243 solution Substances 0.000 description 31
- 239000008098 formaldehyde solution Substances 0.000 description 16
- 210000002966 serum Anatomy 0.000 description 11
- 102000007330 LDL Lipoproteins Human genes 0.000 description 9
- 108010007622 LDL Lipoproteins Proteins 0.000 description 9
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 description 4
- 102000009027 Albumins Human genes 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 4
- 102000008100 Human Serum Albumin Human genes 0.000 description 4
- 108091006905 Human Serum Albumin Proteins 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 229920001577 copolymer Polymers 0.000 description 4
- RIFGWPKJUGCATF-UHFFFAOYSA-N ethyl chloroformate Chemical compound CCOC(Cl)=O RIFGWPKJUGCATF-UHFFFAOYSA-N 0.000 description 4
- 229960001269 glycine hydrochloride Drugs 0.000 description 4
- 238000000760 immunoelectrophoresis Methods 0.000 description 4
- 239000010414 supernatant solution Substances 0.000 description 4
- 238000000108 ultra-filtration Methods 0.000 description 4
- 238000010626 work up procedure Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000003480 eluent Substances 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 108010074605 gamma-Globulins Proteins 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- NFGXHKASABOEEW-UHFFFAOYSA-N 1-methylethyl 11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate Chemical compound COC(C)(C)CCCC(C)CC=CC(C)=CC(=O)OC(C)C NFGXHKASABOEEW-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000012985 polymerization agent Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000009589 serological test Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/806—Antigenic peptides or proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/829—Blood
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT770667A AT279804B (de) | 1967-08-22 | 1967-08-22 | Verfahren zur Isolierung von Antigenen und Antikörpern |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1767899A1 true DE1767899A1 (de) | 1971-09-30 |
Family
ID=3598693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19681767899 Pending DE1767899A1 (de) | 1967-08-22 | 1968-06-28 | Verfahren zur Isolierung von Antigenen und Antikoerpern |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3639559A (enExample) |
| AT (1) | AT279804B (enExample) |
| BE (1) | BE718078A (enExample) |
| DE (1) | DE1767899A1 (enExample) |
| FR (1) | FR1584024A (enExample) |
| IL (1) | IL30583A0 (enExample) |
| NL (1) | NL6810281A (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677132A (en) * | 1971-05-20 | 1997-10-14 | Strahilevitz; Meir | Immunological assay reagent package |
| BE789188A (fr) * | 1971-09-24 | 1973-01-15 | Orion Yhtymae Oy | Procede pour la determination quantitative et qualitative de molecules ayant des proprietes antigenes |
| US4054646A (en) * | 1973-07-30 | 1977-10-18 | General Electric | Method and apparatus for detection of antibodies and antigens |
| CA1025772A (en) | 1972-06-26 | 1978-02-07 | Ivar Giaever | Method and apparatus for detection and purification of proteins and antibodies |
| US4189470A (en) * | 1973-01-30 | 1980-02-19 | Bio-Response, Inc. | Method for the continuous removal of a specific antibody from the lymph fluid in animals and humans |
| US3896217A (en) * | 1973-03-19 | 1975-07-22 | Summa Corp | Method and apparatus for radioimmunoassay with regeneration of immunoadsorbent |
| US4283383A (en) * | 1974-05-20 | 1981-08-11 | Technicon Instruments Corporation | Analysis of biological fluids |
| GB1508132A (en) * | 1974-05-20 | 1978-04-19 | Technicon Instr | Analysis of biological fluids |
| US4041146A (en) * | 1975-05-01 | 1977-08-09 | General Electric Company | Method for detection of biological particles |
| US4123427A (en) * | 1976-07-27 | 1978-10-31 | Daniel Thomas M | Method for the purification of mycobacterial protein antigens and resulting product |
| US20080131954A1 (en) * | 2006-11-30 | 2008-06-05 | Canon U.S. Life Sciences, Inc. | Method of Separating Target DNA from Mixed DNA |
| MX2017002484A (es) | 2014-08-27 | 2017-05-23 | Halliburton Energy Services Inc | Agentes de bloqueo de agua mediante el uso de hidrogeles de proteina de colza hidrolizada. |
-
1967
- 1967-08-22 AT AT770667A patent/AT279804B/de not_active IP Right Cessation
-
1968
- 1968-06-28 DE DE19681767899 patent/DE1767899A1/de active Pending
- 1968-07-09 US US743302A patent/US3639559A/en not_active Expired - Lifetime
- 1968-07-12 FR FR1584024D patent/FR1584024A/fr not_active Expired
- 1968-07-15 BE BE718078D patent/BE718078A/xx unknown
- 1968-07-19 NL NL6810281A patent/NL6810281A/xx unknown
- 1968-08-20 IL IL30583A patent/IL30583A0/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BE718078A (enExample) | 1969-01-15 |
| NL6810281A (enExample) | 1969-02-25 |
| FR1584024A (enExample) | 1969-12-12 |
| AT279804B (de) | 1970-03-25 |
| US3639559A (en) | 1972-02-01 |
| IL30583A0 (en) | 1968-10-24 |
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