CY1109759T1 - Παραγωγη ανασυνδυασμενης πρωτεϊνης σε ανθρωπινο κυτταρο η οποια περιλαμβανει τουλαχιστον μια ε1 πρωτεϊνη του αδενοϊου - Google Patents

Παραγωγη ανασυνδυασμενης πρωτεϊνης σε ανθρωπινο κυτταρο η οποια περιλαμβανει τουλαχιστον μια ε1 πρωτεϊνη του αδενοϊου

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Publication number
CY1109759T1
CY1109759T1 CY20101100084T CY101100084T CY1109759T1 CY 1109759 T1 CY1109759 T1 CY 1109759T1 CY 20101100084 T CY20101100084 T CY 20101100084T CY 101100084 T CY101100084 T CY 101100084T CY 1109759 T1 CY1109759 T1 CY 1109759T1
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Cyprus
Prior art keywords
protein
production
human cell
adenosine
human
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CY20101100084T
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English (en)
Inventor
Guus Hateboer
Karina Cornelia Verhulst
Govert Schouten
Alphonsus Uytdehaag
Abraham Bout
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Crucell Holland B.V.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26153302&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1109759(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Crucell Holland B.V. filed Critical Crucell Holland B.V.
Publication of CY1109759T1 publication Critical patent/CY1109759T1/el

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    • C—CHEMISTRY; METALLURGY
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Health & Medical Sciences (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει μεθόδους και συνθέσεις για την παραγωγή ανασυνδυασμένων πρωτεϊνών σε μια ανθρώπινη κυτταρική σειρά. Οι μέθοδοι και οι συνθέσεις είναι ιδιαίτερα χρήσιμες για την δημιουργία σταθερής έκφρασης των ανθρώπινων ανασυνδυασμένων πρωτεϊνών που ενδιαφέρουν οι οποίες είναι τροποποιημένες μετα-μεταφραστικά, π.χ. μέσω γλυκοζυλίωσης. Τέτοιες πρωτεΐνες μπορεί να έχουν καλύτερες ιδιότητες σε σύγκριση με τις αντίστοιχες που παράγονται σε μη-ανθρώπινα συστήματα όπως σε κύτταρα Ωοθηκών Κινέζικων Χάμστερ (CHO). Η παρούσα εφεύρεση παρέχει επίσης ένα κύτταρο προερχόμενο από έναν ανθρώπινο εμβρυονικό ρετινοβλάστη όπου το αναφερθέν κύτταρο περιλαμβάνει στο γονιδίωμά του αλληλουχίες που κωδικοποιούν Ε1Α και Ε1Β ενός αδενοϊού, και το αναφερθέν κύτταρο περαιτέρω περιλαμβάνει ένα cDNA που κωδικοποιεί ένα ένζυμο το οποίον εμπλέκεται σε μετα-μεταφραστική τροποποίηση.
CY20101100084T 1999-04-15 2010-01-27 Παραγωγη ανασυνδυασμενης πρωτεϊνης σε ανθρωπινο κυτταρο η οποια περιλαμβανει τουλαχιστον μια ε1 πρωτεϊνη του αδενοϊου CY1109759T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99201176 1999-04-15
EP99204434 1999-12-21
EP05100732A EP1533380B1 (en) 1999-04-15 2000-04-17 Recombinant protein production in a human cell comprising at least one E1 protein of adenovirus

Publications (1)

Publication Number Publication Date
CY1109759T1 true CY1109759T1 (el) 2014-09-10

Family

ID=26153302

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20101100084T CY1109759T1 (el) 1999-04-15 2010-01-27 Παραγωγη ανασυνδυασμενης πρωτεϊνης σε ανθρωπινο κυτταρο η οποια περιλαμβανει τουλαχιστον μια ε1 πρωτεϊνη του αδενοϊου

Country Status (18)

Country Link
EP (3) EP1533380B1 (el)
JP (1) JP4210036B2 (el)
CN (1) CN100457914C (el)
AT (3) ATE447617T1 (el)
AU (1) AU772352B2 (el)
CA (1) CA2370477C (el)
CY (1) CY1109759T1 (el)
DE (2) DE60043273D1 (el)
DK (3) DK2163640T3 (el)
ES (3) ES2237420T5 (el)
HK (1) HK1048138B (el)
IL (2) IL145849A0 (el)
MX (1) MXPA01010360A (el)
NO (1) NO328951B1 (el)
NZ (1) NZ514951A (el)
PT (2) PT1161548E (el)
SI (2) SI1161548T2 (el)
WO (1) WO2000063403A2 (el)

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