KR960023061A - 재조합 인간 적혈구생성 촉진인자의 제조방법, 이를 위한 벡터 및 동물 세포주 - Google Patents

재조합 인간 적혈구생성 촉진인자의 제조방법, 이를 위한 벡터 및 동물 세포주 Download PDF

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KR960023061A
KR960023061A KR1019940033404A KR19940033404A KR960023061A KR 960023061 A KR960023061 A KR 960023061A KR 1019940033404 A KR1019940033404 A KR 1019940033404A KR 19940033404 A KR19940033404 A KR 19940033404A KR 960023061 A KR960023061 A KR 960023061A
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South Korea
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cell line
animal cell
recombinant human
expression vector
vector
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KR1019940033404A
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KR0141314B1 (ko
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송영호
오영훈
김창환
박기원
이태호
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성재갑
주식회사 Lg 화학
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
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  • Cell Biology (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 재조합 인간 적혈구생성 촉진인자(rhEPO)를 동물 세포 내에서 효과적으로 빠른 시간내에 대량 생산할 수 있는 방법에 관한 것으로, 벡터 증폭용 선택 표지 인자로 인간 글루타민 합성효소(hGS) 유전자를 포함하는 rhEPO 유전자 발현벡터, 상기 발현벡터에 의하여 형질 전환된 영구 세포주 및 이러한 세포주를 사용하여 rhEPO를 대량 생산하는 방법을 제공한다.

Description

재조합 인간 적혈구생성 촉진인자의 제조방법, 이를 위한 벡터 및 동물 세포주
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. 인간 글루타민 합성효소(hGS)유전자를 선택표지인자로 포함하는 것을 특징으로 하는 재조합 인간 적혈구 생성 촉진 인자(rhEPO) 유전자 발현벡터.
  2. 제1항에 있어서, 발현벡터 pMI-EPO60-hGS.
  3. 제1항 또는 제2항의 발현벡터에 의해 형질전환된 동물 세포주.
  4. 제3항에 있어서, 동물 세포주 CHO-KI-EPO(Chinese Hamster Ovary-KI-EPO) (기탁번호 KCTC 0124BP).
  5. 제3항의 동물 세포주를 배양하는 것을 포함하는, 재조합 인간 적혈구 생성 촉진 인자의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940033404A 1994-12-09 1994-12-09 재조합 인간 적혈구생성 촉진인자 제조방법, 이를 위한 벡터 및 동물 세포주 KR0141314B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940033404A KR0141314B1 (ko) 1994-12-09 1994-12-09 재조합 인간 적혈구생성 촉진인자 제조방법, 이를 위한 벡터 및 동물 세포주

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940033404A KR0141314B1 (ko) 1994-12-09 1994-12-09 재조합 인간 적혈구생성 촉진인자 제조방법, 이를 위한 벡터 및 동물 세포주

Publications (2)

Publication Number Publication Date
KR960023061A true KR960023061A (ko) 1996-07-18
KR0141314B1 KR0141314B1 (ko) 1998-06-15

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KR1019940033404A KR0141314B1 (ko) 1994-12-09 1994-12-09 재조합 인간 적혈구생성 촉진인자 제조방법, 이를 위한 벡터 및 동물 세포주

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KR (1) KR0141314B1 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102141314B1 (ko) 2019-08-29 2020-08-04 김규상 전도방지가 가능한 말뚝 인양장치

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