CS374391A3 - 3'-/2'/-amino- or thiol modified, with a fluorescent dye coupled nucleosides, nucleotides and oligonucleotides, process of their preparation and their use - Google Patents

3'-/2'/-amino- or thiol modified, with a fluorescent dye coupled nucleosides, nucleotides and oligonucleotides, process of their preparation and their use

Info

Publication number
CS374391A3
CS374391A3 CS913743A CS374391A CS374391A3 CS 374391 A3 CS374391 A3 CS 374391A3 CS 913743 A CS913743 A CS 913743A CS 374391 A CS374391 A CS 374391A CS 374391 A3 CS374391 A3 CS 374391A3
Authority
CS
Czechoslovakia
Prior art keywords
oligonucleotides
amino
nucleotides
fluorescent dye
preparation
Prior art date
Application number
CS913743A
Other languages
Czech (cs)
English (en)
Inventor
Joachim Engels
Mathias Herrlein
Renate Konrad
Matthias Mag
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of CS374391A3 publication Critical patent/CS374391A3/cs

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CS913743A 1990-12-11 1991-12-10 3'-/2'/-amino- or thiol modified, with a fluorescent dye coupled nucleosides, nucleotides and oligonucleotides, process of their preparation and their use CS374391A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4039488 1990-12-11

Publications (1)

Publication Number Publication Date
CS374391A3 true CS374391A3 (en) 1992-06-17

Family

ID=6420050

Family Applications (1)

Application Number Title Priority Date Filing Date
CS913743A CS374391A3 (en) 1990-12-11 1991-12-10 3'-/2'/-amino- or thiol modified, with a fluorescent dye coupled nucleosides, nucleotides and oligonucleotides, process of their preparation and their use

Country Status (27)

Country Link
US (3) US5679785A (pt)
EP (1) EP0490281B1 (pt)
JP (1) JP3140127B2 (pt)
KR (1) KR920012107A (pt)
AT (1) ATE142222T1 (pt)
AU (1) AU643856B2 (pt)
BG (1) BG61484B1 (pt)
BR (1) BR9105335A (pt)
CA (1) CA2057475A1 (pt)
CS (1) CS374391A3 (pt)
DE (1) DE59108143D1 (pt)
DK (1) DK0490281T3 (pt)
ES (1) ES2093671T3 (pt)
FI (1) FI915779A7 (pt)
GR (1) GR3021041T3 (pt)
HU (1) HUT60505A (pt)
IE (1) IE75726B1 (pt)
IL (1) IL100298A (pt)
MX (1) MX9102468A (pt)
NO (1) NO914859L (pt)
NZ (1) NZ240900A (pt)
PL (1) PL168874B1 (pt)
PT (1) PT99747A (pt)
RO (1) RO111575B1 (pt)
RU (1) RU2073682C1 (pt)
TR (1) TR27715A (pt)
YU (1) YU187991A (pt)

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US5599922A (en) * 1994-03-18 1997-02-04 Lynx Therapeutics, Inc. Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties
US5726297A (en) * 1994-03-18 1998-03-10 Lynx Therapeutics, Inc. Oligodeoxyribonucleotide N3' P5' phosphoramidates
DE4418691A1 (de) * 1994-05-28 1996-02-22 Boehringer Mannheim Gmbh 3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5859233A (en) * 1996-02-21 1999-01-12 Lynx Therapeutics, Inc. Synthons for synthesis of oligonucleotide N3-P5 phosphoramidates
US5684143A (en) * 1996-02-21 1997-11-04 Lynx Therapeutics, Inc. Oligo-2'-fluoronucleotide N3'->P5' phosphoramidates
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
DE19741084A1 (de) * 1997-09-18 1999-03-25 Knoell Hans Forschung Ev Hochaffine Nukleinsäuremoleküle mit funktionellen Gruppen zur spezifischen Erkennung von Zielmolekülen, ihre Herstellung und Verwendung
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US6761877B2 (en) * 2000-02-18 2004-07-13 Biocrystal, Ltd. Functionalized encapsulated fluorescent nanocrystals
ATE352586T2 (de) 2000-09-29 2007-02-15 Molecular Probes Inc Modifizierte carbocyaninfarbstoffe und deren konjugate
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KR100762261B1 (ko) * 2001-04-27 2007-10-04 (주)바이오니아 전장 상보 디옥시리보핵산 제조 방법과 이에 사용되는앵커와 프라이머
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US7205048B2 (en) * 2001-09-17 2007-04-17 Invitrogen Corporation Functionalized fluorescent nanocrystal compositions and methods of making
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
JP4177123B2 (ja) * 2003-01-10 2008-11-05 富士通株式会社 配線図形検証方法、プログラム及び装置
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US20050131224A1 (en) * 2003-12-15 2005-06-16 Cti Pet Systems, Inc. Method for preparing radiolabeled thymidine
US7160537B2 (en) * 2003-12-15 2007-01-09 Siemens Medical Solutions Usa, Inc. Method for preparing radiolabeled thymidine having low chromophoric byproducts
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
EP1797224B1 (en) * 2004-08-17 2012-10-03 Life Technologies Corporation Synthesis of highly luminescent colloidal particles
ATE556126T1 (de) 2004-10-29 2012-05-15 Life Technologies Corp Funktionalisierte fluoreszierende nanokristalle und verfahren zu deren herstellung und verwendung
WO2006048262A2 (en) * 2004-11-04 2006-05-11 Roche Diagnostics Gmbh Classification of acute myeloid leukemia
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
EP2272983A1 (en) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US7700289B2 (en) * 2006-04-03 2010-04-20 Agilent Technologies, Inc. Method for labeling RNA
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US7572585B2 (en) * 2006-07-31 2009-08-11 Agilent Technologies, Inc. Enzymatic labeling of RNA
RU2326888C1 (ru) * 2006-10-23 2008-06-20 Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (СО РАН) Способ получения солей 5'-трифосфатов дезоксирибо- и рибоолигонуклеотидов
CN102443030B (zh) * 2006-12-05 2015-12-16 激光基因公司 光可断裂的标记核苷酸和核苷与标记的核苷酸和核苷以及其在dna测序中的使用方法
ES2453108T3 (es) 2007-08-06 2014-04-04 Orion Genomics, Llc Nuevos polimorfismos de un único nucleótido y combinaciones de polimorfismos nuevos y conocidos para determinar la expresión específica de alelo del gen IGF2
CA2639416C (en) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Diagnostic test for susceptibility to b-raf kinase inhibitors
WO2009046149A1 (en) * 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
EP2396429B1 (en) 2009-02-11 2015-05-27 Orion Genomics, LLC Combinations of polymorphisms for determining allele-specific expression of igf2
US8431416B2 (en) 2009-04-01 2013-04-30 Becton, Dickinson And Company Reactive heterocycle-substituted 7-hydroxycoumarins and their conjugates
JP5730311B2 (ja) 2009-08-31 2015-06-10 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 反応性シアニン化合物
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
CA2801468C (en) 2010-06-04 2018-09-04 Chronix Biomedical Prostate cancer associated circulating nucleic acid biomarkers
US8623324B2 (en) 2010-07-21 2014-01-07 Aat Bioquest Inc. Luminescent dyes with a water-soluble intramolecular bridge and their biological conjugates
EP2768985B1 (en) 2011-10-21 2019-03-20 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
ES2413566B1 (es) 2011-12-14 2014-05-20 Fundación Pública Andaluza Progreso Y Salud Método de obtención de datos útiles para el diagnóstico y pronóstico de la hipoacusia neurosensorial.
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
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US20160115541A1 (en) 2013-05-29 2016-04-28 Chronix Biomedical Detection and quantification of donor cell-free dna in the circulation of organ transplant recipients
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US4849513A (en) * 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
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Also Published As

Publication number Publication date
RU2073682C1 (ru) 1997-02-20
NO914859D0 (no) 1991-12-10
US5659025A (en) 1997-08-19
HUT60505A (en) 1992-09-28
FI915779A7 (fi) 1992-06-12
GR3021041T3 (en) 1996-12-31
ES2093671T3 (es) 1997-01-01
NO914859L (no) 1992-06-12
AU8892791A (en) 1992-06-18
MX9102468A (es) 1992-06-01
IL100298A (en) 1997-03-18
IL100298A0 (en) 1992-09-06
BG95575A (bg) 1993-12-24
PL168874B1 (pl) 1996-04-30
HU913885D0 (en) 1992-02-28
IE75726B1 (en) 1997-09-24
EP0490281A1 (de) 1992-06-17
DE59108143D1 (de) 1996-10-10
NZ240900A (en) 1994-07-26
DK0490281T3 (pt) 1997-02-10
BG61484B1 (bg) 1997-09-30
US5679785A (en) 1997-10-21
ATE142222T1 (de) 1996-09-15
PT99747A (pt) 1992-11-30
FI915779A0 (fi) 1991-12-09
EP0490281B1 (de) 1996-09-04
JPH04290896A (ja) 1992-10-15
JP3140127B2 (ja) 2001-03-05
AU643856B2 (en) 1993-11-25
KR920012107A (ko) 1992-07-25
CA2057475A1 (en) 1992-06-12
BR9105335A (pt) 1992-08-25
YU187991A (sh) 1994-09-09
IE914301A1 (en) 1992-06-17
TR27715A (tr) 1995-06-22
US5668269A (en) 1997-09-16
RO111575B1 (ro) 1996-11-29
PL292701A1 (en) 1992-09-21

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