BG95575A - 3'-(2')-амино-и тиолмодифицирани, свързани с флуоресциращо багрило нуклеозиди,нуклеотиди и олигонуклеотиди и метод за тяхното получаване и приложението им - Google Patents

3'-(2')-амино-и тиолмодифицирани, свързани с флуоресциращо багрило нуклеозиди,нуклеотиди и олигонуклеотиди и метод за тяхното получаване и приложението им

Info

Publication number
BG95575A
BG95575A BG095575A BG9557591A BG95575A BG 95575 A BG95575 A BG 95575A BG 095575 A BG095575 A BG 095575A BG 9557591 A BG9557591 A BG 9557591A BG 95575 A BG95575 A BG 95575A
Authority
BG
Bulgaria
Prior art keywords
thiol
group
amino
oligonucleotides
nucleotides
Prior art date
Application number
BG095575A
Other languages
English (en)
Other versions
BG61484B1 (bg
Inventor
Joachim Engels
Mathias Herrlein
Renate Konrad
Matthias Mag
Original Assignee
Hoechst Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Aktiengesellschaft filed Critical Hoechst Aktiengesellschaft
Publication of BG95575A publication Critical patent/BG95575A/bg
Publication of BG61484B1 publication Critical patent/BG61484B1/bg

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

1. 3'-(2')-амино- и тиолмодифицирани нуклеозиди, нуклеотиди и олигонуклеотиди с формула в която R1 означава пуринова или пиримидинова база, поне един от остатъците R2 и R3 е флуоресциращо багрило в или позиция, свързано чрез аминогрупа или тиолова група, а другият остатък в даден случай означава водород, хидроксилна група, защитна хидроксилна или метоксигрупа в позиция или , n има стойност 0, R4 e 5'-защитна група или фосфат, пирофосфат или трифосфат, R5 - кислород, флуорметилен, дифлуорметилен или метилен, R6 - хидроксилна или метоксигрупа или водород в позиция или , като R1, R5 и R6 могат съответно да имат еднакви или различни значения и Y и X могат да бъдат еднакви или различни и означават кислород, сяра, NH или метилен. 6 претенции
BG95575A 1990-12-11 1991-12-04 3'-(2')-амино-и тиолмодифицирани, свързани с флуоресциращо багрило нуклеозиди,нуклеотиди и олигонуклеотиди и метод за тяхното получаване и приложението им BG61484B1 (bg)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4039488 1990-12-11

Publications (2)

Publication Number Publication Date
BG95575A true BG95575A (bg) 1993-12-24
BG61484B1 BG61484B1 (bg) 1997-09-30

Family

ID=6420050

Family Applications (1)

Application Number Title Priority Date Filing Date
BG95575A BG61484B1 (bg) 1990-12-11 1991-12-04 3'-(2')-амино-и тиолмодифицирани, свързани с флуоресциращо багрило нуклеозиди,нуклеотиди и олигонуклеотиди и метод за тяхното получаване и приложението им

Country Status (27)

Country Link
US (3) US5679785A (bg)
EP (1) EP0490281B1 (bg)
JP (1) JP3140127B2 (bg)
KR (1) KR920012107A (bg)
AT (1) ATE142222T1 (bg)
AU (1) AU643856B2 (bg)
BG (1) BG61484B1 (bg)
BR (1) BR9105335A (bg)
CA (1) CA2057475A1 (bg)
CS (1) CS374391A3 (bg)
DE (1) DE59108143D1 (bg)
DK (1) DK0490281T3 (bg)
ES (1) ES2093671T3 (bg)
FI (1) FI915779A (bg)
GR (1) GR3021041T3 (bg)
HU (1) HUT60505A (bg)
IE (1) IE75726B1 (bg)
IL (1) IL100298A (bg)
MX (1) MX9102468A (bg)
NO (1) NO914859L (bg)
NZ (1) NZ240900A (bg)
PL (1) PL168874B1 (bg)
PT (1) PT99747A (bg)
RO (1) RO111575B1 (bg)
RU (1) RU2073682C1 (bg)
TR (1) TR27715A (bg)
YU (1) YU187991A (bg)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5476925A (en) * 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
US5726297A (en) * 1994-03-18 1998-03-10 Lynx Therapeutics, Inc. Oligodeoxyribonucleotide N3' P5' phosphoramidates
US5599922A (en) * 1994-03-18 1997-02-04 Lynx Therapeutics, Inc. Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties
JP3482209B2 (ja) * 1994-03-18 2003-12-22 ジェロン・コーポレーション オリゴヌクレオチドｎ３’→ｐ５’ホスホルアミデート：合成および化合物；ハイブリダイゼーションおよびヌクレアーゼ耐性特性
DE4418691A1 (de) * 1994-05-28 1996-02-22 Boehringer Mannheim Gmbh 3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5859233A (en) * 1996-02-21 1999-01-12 Lynx Therapeutics, Inc. Synthons for synthesis of oligonucleotide N3-P5 phosphoramidates
US5684143A (en) * 1996-02-21 1997-11-04 Lynx Therapeutics, Inc. Oligo-2'-fluoronucleotide N3'->P5' phosphoramidates
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
DE19741084A1 (de) * 1997-09-18 1999-03-25 Knoell Hans Forschung Ev Hochaffine Nukleinsäuremoleküle mit funktionellen Gruppen zur spezifischen Erkennung von Zielmolekülen, ihre Herstellung und Verwendung
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6184347B1 (en) 1998-11-19 2001-02-06 Agilent Technologies Inc. Minimization of blooming in high-density arrays by using reactive wash reagents
US6761877B2 (en) * 2000-02-18 2004-07-13 Biocrystal, Ltd. Functionalized encapsulated fluorescent nanocrystals
CA2423806C (en) * 2000-09-29 2009-12-22 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
US7563618B2 (en) 2001-03-23 2009-07-21 Geron Corporation Oligonucleotide conjugates
KR100762261B1 (ko) 2001-04-27 2007-10-04 (주)바이오니아 전장 상보 디옥시리보핵산 제조 방법과 이에 사용되는앵커와 프라이머
US7205048B2 (en) * 2001-09-17 2007-04-17 Invitrogen Corporation Functionalized fluorescent nanocrystal compositions and methods of making
EP1456022B1 (en) * 2001-09-17 2009-11-11 Life Technologies Corporation Nanocrystals
US7214428B2 (en) * 2001-09-17 2007-05-08 Invitrogen Corporation Highly luminescent functionalized semiconductor nanocrystals for biological and physical applications
AU2003297859A1 (en) * 2002-12-13 2004-07-09 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
JP4177123B2 (ja) * 2003-01-10 2008-11-05 富士通株式会社 配線図形検証方法、プログラム及び装置
KR100890885B1 (ko) 2003-03-31 2009-03-31 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US20050131224A1 (en) * 2003-12-15 2005-06-16 Cti Pet Systems, Inc. Method for preparing radiolabeled thymidine
US7160537B2 (en) * 2003-12-15 2007-01-09 Siemens Medical Solutions Usa, Inc. Method for preparing radiolabeled thymidine having low chromophoric byproducts
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2006033732A1 (en) * 2004-08-17 2006-03-30 Invitrogen Corporation Synthesis of highly luminescent colloidal particles
JP2008519108A (ja) * 2004-10-29 2008-06-05 モレキュラー プローブス， インコーポレイテッド 官能化蛍光性ナノ結晶、並びにそれらの調製及び使用方法
US20090118132A1 (en) * 2004-11-04 2009-05-07 Roche Molecular Systems, Inc. Classification of Acute Myeloid Leukemia
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
JP2008528040A (ja) 2005-02-01 2008-07-31 アジェンコート バイオサイエンス コーポレイション ビーズベースの配列決定のための試薬、方法およびライブラリー
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US7700289B2 (en) * 2006-04-03 2010-04-20 Agilent Technologies, Inc. Method for labeling RNA
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US7572585B2 (en) * 2006-07-31 2009-08-11 Agilent Technologies, Inc. Enzymatic labeling of RNA
KR101504640B1 (ko) * 2006-12-05 2015-03-20 레이저젠, 인코퍼레이티드 광절단가능 표지된 뉴클레오티드와 뉴클레오시드, 표지된 뉴클레오티드와 뉴클레오시드, 그리고 ｄｎａ 염기서열분석에서 이들의 이용 방법
EP2700723B1 (en) 2007-08-06 2015-07-15 Orion Genomics, LLC Novel single nucleotide polymorphisms and combinations of novel and known polymorphisms for determining the allele-specific expression of the IGF2 gene
CA2639416C (en) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Diagnostic test for susceptibility to b-raf kinase inhibitors
EP2212434A1 (en) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase ligation sequencing
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
CA2751758A1 (en) 2009-02-11 2010-08-19 Orion Genomics Llc Combinations of polymorphisms for determining allele-specific expression of igf2
US8431416B2 (en) 2009-04-01 2013-04-30 Becton, Dickinson And Company Reactive heterocycle-substituted 7-hydroxycoumarins and their conjugates
US8309059B2 (en) * 2009-08-31 2012-11-13 Promega Corporation Reactive cyanine compounds
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
PL2576837T3 (pl) 2010-06-04 2018-04-30 Chronix Biomedical Krążeniowe biomarkery typu kwasu nukleinowego związane z rakiem prostaty
US8623324B2 (en) 2010-07-21 2014-01-07 Aat Bioquest Inc. Luminescent dyes with a water-soluble intramolecular bridge and their biological conjugates
US20140303008A1 (en) 2011-10-21 2014-10-09 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
ES2413566B1 (es) 2011-12-14 2014-05-20 Fundación Pública Andaluza Progreso Y Salud Método de obtención de datos útiles para el diagnóstico y pronóstico de la hipoacusia neurosensorial.
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
WO2014093825A1 (en) 2012-12-14 2014-06-19 Chronix Biomedical Personalized biomarkers for cancer
ES2704682T5 (es) 2013-05-29 2023-11-10 Chronix Biomedical Detección y cuantificación de ADN extracelular del donante en la circulación de receptores de trasplantes de órganos
ES2791328T3 (es) 2014-10-01 2020-11-03 Chronix Biomedical Métodos de cuantificación de ADN acelular
KR20180136436A (ko) 2016-03-28 2018-12-24 에이에이티 바이오퀘스트, 인코포레이티드 폴리플루오레노[4,5-cde]옥세핀 접합체 및 피분석물 검출 방법에서의 이의 용도
US11220628B2 (en) 2019-02-20 2022-01-11 Aat Bioquest, Inc. Condensed polycyclic conjugated polymers and their use for biological detection
WO2021154933A1 (en) 2020-01-30 2021-08-05 Aat Bioquest, Inc. Uv excitable polyfluorene based conjugates and their use in methods of analyte detection
WO2023147355A2 (en) * 2022-01-25 2023-08-03 Oregon Health & Science University Methods and systems for increasing capture potential of a spatial array

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5015733A (en) * 1983-12-20 1991-05-14 California Institute Of Technology Nucleosides possessing blocked aliphatic amino groups
US4849513A (en) * 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
EP0267996A1 (en) * 1986-11-20 1988-05-25 Tamir Biotechnology Ltd. New nucleotide derivatives
GB2236852B (en) * 1989-09-25 1994-04-06 Scotgen Ltd DNA probe based assays and intermediates useful in the synthesis of cleavable nucleic acids for use in such assays
US5227487A (en) * 1990-04-16 1993-07-13 Molecular Probes, Inc. Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes
US5541307A (en) * 1990-07-27 1996-07-30 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs and solid phase synthesis thereof

Also Published As

Publication number Publication date
IE914301A1 (en) 1992-06-17
DE59108143D1 (de) 1996-10-10
PL292701A1 (en) 1992-09-21
FI915779A0 (fi) 1991-12-09
CA2057475A1 (en) 1992-06-12
GR3021041T3 (en) 1996-12-31
CS374391A3 (en) 1992-06-17
JP3140127B2 (ja) 2001-03-05
ES2093671T3 (es) 1997-01-01
NZ240900A (en) 1994-07-26
NO914859L (no) 1992-06-12
US5679785A (en) 1997-10-21
RU2073682C1 (ru) 1997-02-20
AU8892791A (en) 1992-06-18
HU913885D0 (en) 1992-02-28
BR9105335A (pt) 1992-08-25
PT99747A (pt) 1992-11-30
AU643856B2 (en) 1993-11-25
BG61484B1 (bg) 1997-09-30
HUT60505A (en) 1992-09-28
ATE142222T1 (de) 1996-09-15
US5659025A (en) 1997-08-19
US5668269A (en) 1997-09-16
IE75726B1 (en) 1997-09-24
RO111575B1 (ro) 1996-11-29
NO914859D0 (no) 1991-12-10
IL100298A0 (en) 1992-09-06
FI915779A (fi) 1992-06-12
EP0490281A1 (de) 1992-06-17
YU187991A (sh) 1994-09-09
IL100298A (en) 1997-03-18
MX9102468A (es) 1992-06-01
KR920012107A (ko) 1992-07-25
DK0490281T3 (bg) 1997-02-10
TR27715A (tr) 1995-06-22
EP0490281B1 (de) 1996-09-04
JPH04290896A (ja) 1992-10-15
PL168874B1 (pl) 1996-04-30

Similar Documents

Publication Publication Date Title
BG95575A (bg) 3'-(2')-амино-и тиолмодифицирани, свързани с флуоресциращо багрило нуклеозиди,нуклеотиди и олигонуклеотиди и метод за тяхното получаване и приложението им
FI104176B1 (fi) Menetelmä nukleosidien uusien antiviraalisten forfolipidijohdannaisten valmistamiseksi
DE3854060D1 (de) Desaza-purin-nucleosid-Derivate, Verfahren zu deren Herstellung sowie deren Verwendung bei der Nucleinsäure-Sequenzierung sowie als antivirale Mittel.
DK0626387T3 (da) Nukleosider og oligonukleotider med 2'-ethergrupper
DK0679657T3 (da) Nukleosider og oligonukleotider med 2'-ethergrupper
ES2033774T3 (es) Derivados antiviricos fosfonometoxialquilenicos de purina y pirimidina.
DE3670293D1 (de) Adenosin-nukleosid-derivate, ihre herstellung und biologische verwendung.
ATE134644T1 (de) Weitere antivirale pyrimidin-nukleosiden
ES2074637T3 (es) Derivados de (fosfonometoxi)metoxi purina/pirimidina antiviricos.
GR3035589T3 (en) Novel CC-1065 analogs having two CPI subunits.
ES8802363A1 (es) Procedimiento para la fabricacion de derivados nucleosidos
DE69024804D1 (de) Antivirale zusammensetzung
RU94002329A (ru) Модифицированные олигодезоксирибонуклеотиды, композиция, способ лечения и промежуточные соединения
ATE44961T1 (de) Substituierte o-sulfonyl-glycosylamide, verfahren zu ihrer herstellung sowie ihre verwendung als arzneimittel.
DE69600739D1 (de) Salpetersäureester von 2-(2,6-Dihalophenylamino) Phenylacetoessigsäurederivaten und Verfahren zu ihrer Herstellung
IT1215339B (it) Procedimento per la preparazione di 9-(idrossialchil)-ipoxantine
FR2665362B1 (fr) Substance contenant, comme principe actif, une adenosine substitutee en n6 et son application.
FR2574798B1 (fr) Nouvelle adenosine substituee en n6 et medicament la contenant comme principe actif
DK0635021T3 (da) Fremgangsmåde til fremstilling af derivater af bis(aminomethyl)phosphinsyre
NO20002168L (no) FremgangsmÕte for fremstilling av chirale nukleosidanaloger
KR910007954A (ko) 신규 이노신/구아노신 유도체
ES455980A1 (es) Un procedimiento para preparar una purina sustituida
JPS56142298A (en) Oligonucleotide derivative and its preparation
TH9308EX (th) พิริมิดีนคลีโอไซด์ที่ใช้ต้านไวรัส