CR20180525A - Producción de ácido ribonucleico libre de células - Google Patents

Producción de ácido ribonucleico libre de células

Info

Publication number
CR20180525A
CR20180525A CR20180525A CR20180525A CR20180525A CR 20180525 A CR20180525 A CR 20180525A CR 20180525 A CR20180525 A CR 20180525A CR 20180525 A CR20180525 A CR 20180525A CR 20180525 A CR20180525 A CR 20180525A
Authority
CR
Costa Rica
Prior art keywords
ribonucleic acid
cells
acid production
production free
free
Prior art date
Application number
CR20180525A
Other languages
English (en)
Inventor
William Jeremy; Blake
Daniel; Maceachran
Mehak; Gupta
Drew S ; Cunningham
James Robbins; Abshire
Original Assignee
Greenlight Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenlight Biosciences Inc filed Critical Greenlight Biosciences Inc
Publication of CR20180525A publication Critical patent/CR20180525A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001—Polyphosphate kinase (2.7.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04006—Nucleoside-diphosphate kinase (2.7.4.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/13—Exoribonucleases producing 5'-phosphomonoesters (3.1.13)
    • C12Y301/13001—Exoribonuclease II (3.1.13.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)

Abstract

Proporcionada en la presente, en algunos aspectos, son métodos y composiciones para la producción de ácido ribonucleico libre de células.
CR20180525A 2016-04-06 2017-04-06 Producción de ácido ribonucleico libre de células CR20180525A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662319220P 2016-04-06 2016-04-06
US201762452550P 2017-01-31 2017-01-31
PCT/US2017/026285 WO2017176963A1 (en) 2016-04-06 2017-04-06 Cell-free production of ribonucleic acid

Publications (1)

Publication Number Publication Date
CR20180525A true CR20180525A (es) 2019-02-14

Family

ID=58672667

Family Applications (1)

Application Number Title Priority Date Filing Date
CR20180525A CR20180525A (es) 2016-04-06 2017-04-06 Producción de ácido ribonucleico libre de células

Country Status (16)

Country Link
US (2) US10954541B2 (es)
EP (2) EP4293104A3 (es)
JP (2) JP7011599B2 (es)
KR (2) KR20230079463A (es)
CN (1) CN109196109B (es)
AU (2) AU2017246458B2 (es)
BR (1) BR112018070506A2 (es)
CA (1) CA3020312A1 (es)
CL (1) CL2018002852A1 (es)
CR (1) CR20180525A (es)
IL (1) IL262138A (es)
MX (1) MX2023001189A (es)
MY (1) MY195729A (es)
RU (1) RU2018138975A (es)
SG (1) SG11201808721YA (es)
WO (1) WO2017176963A1 (es)

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MY193444A (en) 2015-03-30 2022-10-13 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
ES2921017T3 (es) 2016-12-30 2022-08-16 Natures Toolbox Inc Sistema de expresión libre de células que tiene una nueva regeneración de energía basada en polifosfato inorgánico
KR20190100386A (ko) 2017-01-06 2019-08-28 그린라이트 바이오사이언시스, 아이엔씨. 당의 무세포 생산
KR102278495B1 (ko) * 2017-07-05 2021-07-15 오리시로 제노믹스 가부시키가이샤 Dna 생산 방법 및 dna 단편 연결용 키트
KR102571743B1 (ko) * 2017-10-11 2023-08-29 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
WO2019099644A1 (en) * 2017-11-15 2019-05-23 Board Of Regents, The University Of Texas System Methods and kits for using recombinant microorganisms as direct reagents in biological applications
CN108795958B (zh) * 2018-07-11 2020-04-07 南京工业大学 一株表达多聚磷酸激酶的重组菌及其应用
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TWI734095B (zh) * 2019-04-11 2021-07-21 國立清華大學 ｄＮＭＰ激酶及使用此激酶製備核苷酸的方法
CN110702831B (zh) * 2019-11-18 2020-06-16 天津汉科生物科技有限公司 一种超高效液相色谱-串联质谱检测血清睾酮激素的试剂盒
CN110819672A (zh) * 2019-12-04 2020-02-21 美亚药业海安有限公司 一种固定化酶法制备三磷酸胞苷的方法
CN110714043A (zh) * 2019-12-04 2020-01-21 美亚药业海安有限公司 一种固定化酶法制备三磷酸鸟苷的方法
CN113122593A (zh) * 2019-12-31 2021-07-16 安徽古特生物科技有限公司 一种利用多聚磷酸盐制备核苷三磷酸和脱氧核苷三磷酸的方法
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