CL2014003146A1 - Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus. - Google Patents

Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus.

Info

Publication number
CL2014003146A1
CL2014003146A1 CL2014003146A CL2014003146A CL2014003146A1 CL 2014003146 A1 CL2014003146 A1 CL 2014003146A1 CL 2014003146 A CL2014003146 A CL 2014003146A CL 2014003146 A CL2014003146 A CL 2014003146A CL 2014003146 A1 CL2014003146 A1 CL 2014003146A1
Authority
CL
Chile
Prior art keywords
arn
virus
urg
autogenous
pss
Prior art date
Application number
CL2014003146A
Other languages
English (en)
Inventor
San Martín Marcelo Cortez
Ascuy Daniela Toro
Zamorano Carolina Tambley
Pavez Carolina Beltrán
Ossa Eugenio Spencer
Original Assignee
Univ Santiago Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Santiago Chile filed Critical Univ Santiago Chile
Priority to CL2014003146A priority Critical patent/CL2014003146A1/es
Publication of CL2014003146A1 publication Critical patent/CL2014003146A1/es
Priority to CN201680006549.9A priority patent/CN107532180A/zh
Priority to BR112017010630-2A priority patent/BR112017010630A2/pt
Priority to US15/528,328 priority patent/US10544398B2/en
Priority to DK16723238.8T priority patent/DK3222730T3/da
Priority to AU2016203557A priority patent/AU2016203557B2/en
Priority to RU2017121232A priority patent/RU2708151C2/ru
Priority to PCT/CL2016/050002 priority patent/WO2016077938A1/es
Priority to CA2968430A priority patent/CA2968430C/en
Priority to EP16723238.8A priority patent/EP3222730B8/en
Priority to MX2017006671A priority patent/MX2017006671A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2760/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C12N2760/16152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
CL2014003146A 2014-11-20 2014-11-20 Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus. CL2014003146A1 (es)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CL2014003146A CL2014003146A1 (es) 2014-11-20 2014-11-20 Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus.
MX2017006671A MX2017006671A (es) 2014-11-20 2016-01-06 Plásmidos y método para obtener partículas virales.
DK16723238.8T DK3222730T3 (da) 2014-11-20 2016-01-06 Plasmider og fremgangsmåde til opnåelse af virale partikler
BR112017010630-2A BR112017010630A2 (pt) 2014-11-20 2016-01-06 plasmídeos e método para obter partículas virais
US15/528,328 US10544398B2 (en) 2014-11-20 2016-01-06 Plasmids and method for obtaining viral particles
CN201680006549.9A CN107532180A (zh) 2014-11-20 2016-01-06 用于获得病毒颗粒的质粒和方法
AU2016203557A AU2016203557B2 (en) 2014-11-20 2016-01-06 Plasmids and method for obtaining viral particles
RU2017121232A RU2708151C2 (ru) 2014-11-20 2016-01-06 Плазмиды и способ получения вирусных частиц
PCT/CL2016/050002 WO2016077938A1 (es) 2014-11-20 2016-01-06 Plásmidos y método para obtener partículas virales
CA2968430A CA2968430C (en) 2014-11-20 2016-01-06 Plasmids and method for obtaining viral particles
EP16723238.8A EP3222730B8 (en) 2014-11-20 2016-01-06 Plasmids and method for obtaining viral particles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2014003146A CL2014003146A1 (es) 2014-11-20 2014-11-20 Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus.

Publications (1)

Publication Number Publication Date
CL2014003146A1 true CL2014003146A1 (es) 2015-06-12

Family

ID=56013004

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2014003146A CL2014003146A1 (es) 2014-11-20 2014-11-20 Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus.

Country Status (11)

Country Link
US (1) US10544398B2 (es)
EP (1) EP3222730B8 (es)
CN (1) CN107532180A (es)
AU (1) AU2016203557B2 (es)
BR (1) BR112017010630A2 (es)
CA (1) CA2968430C (es)
CL (1) CL2014003146A1 (es)
DK (1) DK3222730T3 (es)
MX (1) MX2017006671A (es)
RU (1) RU2708151C2 (es)
WO (1) WO2016077938A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230048162A (ko) 2015-03-30 2023-04-10 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
AU2017246458B2 (en) 2016-04-06 2022-08-11 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
IL273887B (en) 2017-10-11 2022-09-01 Greenlight Biosciences Inc Methods and preparations for the production of nucleoside triphosphate and ribonucleic acid
WO2024055123A1 (en) * 2022-09-16 2024-03-21 Gmg Fish Services Inc. Vaccines against infectious salmon anemia and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60033284T3 (de) 1999-07-14 2014-10-30 Icahn School Of Medicine At Mount Sinai In vitro-rekonstitution von segmentierten, negativstrang-rna-viren
TWI477602B (zh) * 2006-02-09 2015-03-21 Educational Foundation Jichi Medical Univ Novel viral vector
EP1867725A1 (en) 2006-06-16 2007-12-19 Institut National De La Recherche Agronomique (Inra) Recombinant novirhabdoviruses and uses thereof

Also Published As

Publication number Publication date
EP3222730A1 (en) 2017-09-27
DK3222730T3 (da) 2022-01-31
EP3222730A4 (en) 2018-06-20
CN107532180A (zh) 2018-01-02
AU2016203557A1 (en) 2017-06-15
US10544398B2 (en) 2020-01-28
RU2708151C2 (ru) 2019-12-05
US20180030416A1 (en) 2018-02-01
CA2968430A1 (en) 2016-05-26
CA2968430C (en) 2023-11-07
MX2017006671A (es) 2018-11-09
AU2016203557B2 (en) 2022-02-24
RU2017121232A (ru) 2018-12-20
WO2016077938A1 (es) 2016-05-26
BR112017010630A2 (pt) 2018-02-14
EP3222730B1 (en) 2021-10-27
RU2017121232A3 (es) 2018-12-20
EP3222730B8 (en) 2022-02-23

Similar Documents

Publication Publication Date Title
IL276679B (en) Cells reject viruses and their uses
CL2018001051A1 (es) Vacunas de ácido nucleico contra el virus varicela zoster (vzv)
MX2017007196A (es) Fibroblastos de embrion de pollo inmortalizados.
SG10201803081TA (en) Oncolytic adenovirus encoding a b7 protein
UY35407A (es) ?PROTEÍNAS DE FUSIÓN COMPRENDIENDO PORCIONES PDGF y VEGF DE UNIÓN Y MÉTODOS PARA SU USO?.
BR112016030968A2 (pt) análogos de pridopidina, preparação e uso destes
SG11201606624WA (en) Antibody moleules to dengue virus and uses thereof
EP3187315A4 (en) Razor blade and razor cartridge using same
AR104604A1 (es) ANTICUERPOS ANTI-FcRn
EP3215602A4 (en) Methods and materials for producing recombinant viruses in eukaryotic microalgae
EP3142680A4 (en) Lpa-associated protein and rna expression
CL2016001921A1 (es) Proteínas inmovilizadas y uso de las mismas
EP3194595A4 (en) Viral rna segments as immunomodulatory agents and vaccine components
CL2014003146A1 (es) Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus.
UY35835A (es) Anticuerpos específicos para FcRn.
SG11201607980UA (en) Water-soluble trans-membrane proteins and methods for the preparation and use thereof
ZA201702508B (en) An agrochemical suspension concentrate comprising an alkoxylated alcohol dissolved in the aqueous phase
HK1219500A1 (zh) 遺傳穩定的溶瘤性 病毒、其製備方法及用途
GB201504251D0 (en) Oncolytic herpes simplex virus infected cells
HK1205148A1 (en) Proteins and methods used to prepare hepatitis e virus-like particles
EP3285775A4 (en) Methods and materials for killing hiv infected cells
EP3436065A4 (en) ONCOLYTIC VIRUS COMBINATION THERAPIES AGAINST CANCER AND PLATFORMS FOR THE SELECTION OF FIRST-CLASS RESPONDER
HK1246824A1 (zh) 應用人脂肪衍生的幹細胞增殖血清來源的丙肝病毒及其用途
EP3216868A4 (en) Virus vector, cell, and construct
EP3194602A4 (en) Compounds and methods for enhancing viral gene transfer to human hematopoietic cells