MX2017006671A - Plásmidos y método para obtener partículas virales. - Google Patents

Plásmidos y método para obtener partículas virales.

Info

Publication number
MX2017006671A
MX2017006671A MX2017006671A MX2017006671A MX2017006671A MX 2017006671 A MX2017006671 A MX 2017006671A MX 2017006671 A MX2017006671 A MX 2017006671A MX 2017006671 A MX2017006671 A MX 2017006671A MX 2017006671 A MX2017006671 A MX 2017006671A
Authority
MX
Mexico
Prior art keywords
viral particles
plasmids
obtaining viral
virus
producing
Prior art date
Application number
MX2017006671A
Other languages
English (en)
Inventor
Beltrán Pavez Carolina
Tambley Zamorano Carolina
Toro Ascuy Daniela
Spencer Ossa Eugenio
Cortez San Martín Marcelo
Original Assignee
Univ Santiago Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Santiago Chile filed Critical Univ Santiago Chile
Publication of MX2017006671A publication Critical patent/MX2017006671A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2760/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C12N2760/16152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Metodo para producir virus ARN monocatenario negativo; plasmido recombinante funcional en celulas animales, que consta de un esqueleto pSSURG; metodo de obtencion de particulas virales; y uso para expresar ARN, proteinas autogenas o exogenas al virus.
MX2017006671A 2014-11-20 2016-01-06 Plásmidos y método para obtener partículas virales. MX2017006671A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CL2014003146A CL2014003146A1 (es) 2014-11-20 2014-11-20 Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus.
PCT/CL2016/050002 WO2016077938A1 (es) 2014-11-20 2016-01-06 Plásmidos y método para obtener partículas virales

Publications (1)

Publication Number Publication Date
MX2017006671A true MX2017006671A (es) 2018-11-09

Family

ID=56013004

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017006671A MX2017006671A (es) 2014-11-20 2016-01-06 Plásmidos y método para obtener partículas virales.

Country Status (11)

Country Link
US (1) US10544398B2 (es)
EP (1) EP3222730B8 (es)
CN (1) CN107532180A (es)
AU (1) AU2016203557B2 (es)
BR (1) BR112017010630A2 (es)
CA (1) CA2968430C (es)
CL (1) CL2014003146A1 (es)
DK (1) DK3222730T3 (es)
MX (1) MX2017006671A (es)
RU (1) RU2708151C2 (es)
WO (1) WO2016077938A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2017012665A (es) 2015-03-30 2018-04-24 Greenlight Biosciences Inc Produccion de acido ribonucleico libre de celulas.
EP4293104A3 (en) 2016-04-06 2024-04-24 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
KR20230130144A (ko) 2017-10-11 2023-09-11 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
WO2024055123A1 (en) * 2022-09-16 2024-03-21 Gmg Fish Services Inc. Vaccines against infectious salmon anemia and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1194580B2 (en) 1999-07-14 2010-08-25 Mount Sinai School of Medicine of New York University In vitro reconstitution of segmented negative-strand rna viruses
TWI477602B (zh) * 2006-02-09 2015-03-21 Educational Foundation Jichi Medical Univ Novel viral vector
EP1867725A1 (en) 2006-06-16 2007-12-19 Institut National De La Recherche Agronomique (Inra) Recombinant novirhabdoviruses and uses thereof

Also Published As

Publication number Publication date
BR112017010630A2 (pt) 2018-02-14
EP3222730B1 (en) 2021-10-27
DK3222730T3 (da) 2022-01-31
AU2016203557A1 (en) 2017-06-15
CA2968430C (en) 2023-11-07
EP3222730A4 (en) 2018-06-20
RU2017121232A3 (es) 2018-12-20
AU2016203557B2 (en) 2022-02-24
CL2014003146A1 (es) 2015-06-12
US20180030416A1 (en) 2018-02-01
EP3222730B8 (en) 2022-02-23
RU2017121232A (ru) 2018-12-20
CA2968430A1 (en) 2016-05-26
RU2708151C2 (ru) 2019-12-05
CN107532180A (zh) 2018-01-02
EP3222730A1 (en) 2017-09-27
WO2016077938A1 (es) 2016-05-26
US10544398B2 (en) 2020-01-28

Similar Documents

Publication Publication Date Title
HK1245838A1 (zh) 用於體外病毒基因組工程的組合物和方法
EP3386537A4 (en) MATERIALS AND METHODS OF DISTRIBUTING NUCLEIC ACIDS ON COCHLEA AND VORHOF CELLS
IL260039A (en) Plasmid templates for heterologous protein expression and methods of use
EP3186375A4 (en) NEW CAS9 PROTEINS AND GUIDING ELEMENTS FOR DNA TARGETING AND THE GENOME EDITION
IL249828A0 (en) Limbal stem cells of cultured mammals, methods of their creation and uses thereof
EP3219810A4 (en) Method for detecting off-target site of genetic scissors in genome
EP3133047B8 (en) Lithium metal-skeleton carbon composite and preparation method therefor, negative electrode, and battery
EP3156367A4 (en) Negative electrode active material and method for preparing same
EP3215602A4 (en) Methods and materials for producing recombinant viruses in eukaryotic microalgae
EP2928924A4 (en) HIV-1 ENV BINDING ANTIBODIES, FUSION PROTEINS AND METHODS OF USE
PH12016502555A1 (en) Methods of preparing substituted nucleotide analogs
IL249903A0 (en) Recombinant Listeria strain expressing heterologous antigenic chimeric proteins and methods of using the same
EP3222718A4 (en) Method for knock-in of dna into target region of mammalian genome, and cell
MX2017008504A (es) Vacuna para enfermedad de newcastle y de bursitis infecciosa (nd-ibd) en vector de hvt mejorada.
MX2017014397A (es) Anticuerpos anti-receptor neonatal de fragmento cristalizable(fcrn).
MX2017006671A (es) Plásmidos y método para obtener partículas virales.
EP3194595A4 (en) Viral rna segments as immunomodulatory agents and vaccine components
EP3218397B8 (en) Neutralizing antibodies to ebola virus glycoprotein and their use
PL3386012T3 (pl) Materiał czynny elektrody ujemnej obejmujący materiał złożony na bazie tytanu, sposób jego wytwarzania i zawierający go akumulator litowy
PH12016501689B1 (en) Tatk-cdkl5 fusion proteins, compositions, formulations, and use thereof
HK1259067A1 (zh) 用牛免疫缺陷病毒gag蛋白的重組病毒樣顆粒
SG11201610878QA (en) Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same
MX2016010215A (es) Composiciones y metodos de tratamiento y prevencion de degeneracion macular.
HK1246824A1 (zh) 應用人脂肪衍生的幹細胞增殖血清來源的丙肝病毒及其用途
EP3101035A4 (en) Bifunctional fusion protein, preparation method therefor, and use thereof