CN1596267A

CN1596267A - 来自谷氨酸棒状杆菌的编码调节蛋白的基因

Info

Publication number: CN1596267A
Application number: CNA028235843A
Authority: CN
Inventors: O·策尔德尔; M·蓬佩尤斯; H·施罗德; B·克勒格尔; C·克洛普罗格; G·哈伯豪尔
Original assignee: BASF SE
Current assignee: BASF SE
Priority date: 2001-11-05
Filing date: 2002-10-31
Publication date: 2005-03-16
Also published as: ZA200404417B; KR100861747B1; BR0213775A; DE10154245A1; KR20050042250A; EP1444257A2; AU2002346805A1; WO2003040181A3; US20050014234A1; WO2003040181A2

Abstract

本发明涉及新的核酸分子，涉及其用于构建遗传改良的微生物的用途，还涉及借助于所述遗传改良的微生物制备精细化学品的方法，尤其是制备氨基酸的方法。

Description

来自谷氨酸棒状杆菌的编码调节蛋白的基因

发明背景

细胞中天然进行的代谢过程所产生的特定产物和副产物已用于工业的许多分支中，包括食品工业、动物饲料工业、化妆品工业及药学工业。这些分子合起来称为“精细化学品”，其包括有机酸(蛋白原的和非蛋白原的氨基酸)、核苷酸和核苷、脂类和脂肪酸、二醇、碳水化合物、芳香化合物、维生素和辅因子、以及酶。它们最好的产生是通过大规模培养已研发的在每一特定情况下可产生并分泌大量目标分子的细菌。一种对此目的尤其有用的生物体是谷氨酸棒状杆菌(Corynebacterium glutamicum)，其为革兰氏阳性的非致病菌。通过菌株选择，已开发了许多突变菌株，它们产生多种目标化合物。但是，为提高特定分子产量的菌株选择是一种费时且困难的方法。

发明简述

本发明提供了新的可用于谷氨酸棒状杆菌或相关菌种的鉴定或分类的核酸分子。谷氨酸棒状杆菌为革兰氏阳性需氧细菌，其广泛用在工业上用于大规模产生多种精细化学品，也用于降解碳氢化合物(如在原油泄漏物中)，以及用于氧化萜类化合物。本发明的核酸分子因此可用于鉴定能如通过发酵法用来产生精细化学品的微生物。虽然谷氨酸棒状杆菌自身为非致病的，但它与其它棒状杆菌如对人致病的重要病原菌白喉棒状杆菌(Corynebacterium diphtheriae)(白喉的病原菌)有亲缘关系。所以鉴定存在棒状杆菌的能力也具有显著的临床重要性，例如诊断应用。而且，所述核酸分子可作为对谷氨酸棒状杆菌或相关生物进行基因组作图的参考点。

这些新核酸分子编码的蛋白质文中称为代谢调节(MR)蛋白。这些MR蛋白可以例如发挥参与对细胞的正常代谢功能起关键作用的蛋白的转录、翻译或翻译后调节的功能。由于可得到用于谷氨酸棒状杆菌中的克隆载体(如在Sinskey等人，美国专利号4,649,119中所公开的)和用于遗传操作谷氨酸棒状杆菌和相关短杆菌种(如lactofermentum)的技术(Yoshihama等人，细菌学杂志(J.Bacteriol.)162：(1985)591-597；Katsumata等人，细菌学杂志(J.Bacteriol.)159：(1984)306-311；和Santamaria等人，普通微生物学杂志(J.Gen.Microbiol.)130：(1984)2237-2246)，本发明的核酸分子可用于该生物体的遗传操作，使其成为一种或多种精细化学品的较好和更有效的生产者。

通过操作本发明的基因可以直接或间接地导致精细化学品产量、生产率和/或生产效率的提高。更具体地，对通常调节精细化学品代谢途径中精细化学品的产量、生产率和/或生产效率的谷氨酸棒状杆菌MR蛋白的修饰可能对所述生物体的一种或多种这些所需化合物的总生产率有直接的影响。对参与这些代谢途径的蛋白的修饰可能也对目的精细化学品的产量、生产率和/或生产效率有间接影响。代谢调节不可避免地是复杂的并且影响不同途径的调节机制在许多地方可重叠以致可以根据特定细胞事件快速调节一种以上的代谢途径。这使得可修饰一种代谢途径中的调节蛋白也可影响许多其他代谢途径，它们中的一些可能参与目的精细化学品的生物合成或降解。在这种间接方式中，对MR蛋白的作用进行调节可能影响精细化学品的生产率，所述该精细化学品产生的代谢途径不同于受所述MR蛋白直接调节的代谢途径。

可以使用本发明的核酸和蛋白分子以便直接提高谷氨酸棒状杆菌的一种或多种目的精细化学品的产量、生产率和/或生产效率。可通过本领域已知的基因重组技术操作一种或多种本发明的调节蛋白，从而使得它们的功能被调节。参与抑制编码氨基酸生物合成所需的酶的基因转录使得所述氨基酸不再能够抑制该转录的MR蛋白的突变可导致例如，所述氨基酸生产率的提高。所以，MR蛋白活性的修饰(其导致参与目的精细化学品生物合成的谷氨酸棒状杆菌蛋白的翻译增强或翻译后修饰的活化)接下来可增加所述化学品的生产率。相反的情况可同样有用：通过增强对转录或翻译的抑制或者通过对参与调节化合物降解途径的谷氨酸棒状杆菌蛋白的翻译后负修饰，可增加所述化学品的生产率。在任何一种情况下，可以增加该目的精细化学品的总产量或生产率。

同样，本发明蛋白和核苷酸分子中的所述修饰可通过间接机制提高精细化学品的产量、生产率和/或生产效率。特定化合物的代谢不可避免地与细胞中其他生物合成和降解途径相关，并且代谢途径中的必需辅因子、中间物或底物都可能被另一个代谢途径所提供或限制。所以调节本发明的一种或多种调节蛋白能够影响精细化学品的其他生物合成或降解途径的活性的效率。除此之外，对一种或多种调节蛋白的操作可增加细胞在培养物中，特别是在大规模发酵培养物(其中的生长条件可能不是最优的)中的生长和增殖的总体能力。例如通过突变通常抑制核苷生物合成的本发明MR蛋白，使得所述蛋白具有较低的抑制活性，从而可增加核苷的生物合成和细胞分裂，其中所述核苷生物合成的抑制为对营养的非最优胞外提供而产生的反应(从而阻止细胞分裂)。在那些导致培养物中细胞生长和分裂增加的MR蛋白的修饰可以导致培养物中一种或多种目标精细化学品的产量、生产率和/或生产效率增加，这至少是因为培养物中生产所述化学品的细胞数目的增加。

本发明提供了编码本文中称作代谢调节(MR)蛋白的新核酸分子并且所述蛋白例如能够进行和谷氨酸棒状杆菌中代谢途径的转录、翻译或翻译后调节相关的酶促步骤。本文中编码MR蛋白的核酸分子被称作MR核酸分子。在一个优选的实施方案中，MR蛋白参与一个或多个代谢途径的转录、翻译或翻译后调节。这些蛋白的实例是表1中列出的基因编码的蛋白。

因此，本发明的一个方面涉及这样的分离核酸分子(例如cDNA)，其包含编码MR蛋白或其生物学活性部分的核苷酸序列和适合作为检测或扩增编码MR的核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在尤其优选的实施方案中，该分离的核酸分子包含任何集合A所列出的核苷酸序列或任何这些核苷酸序列的编码区或其互补序列。在其他优选的实施方案中，该分离的核酸分子编码集合B中列出的任何氨基酸序列。本发明优选的MR蛋白同样优选地具有至少一种本文所描述的MR活性。

集合A定义了下面的表1中描述的序列表的核酸序列以及相关位置的序列修饰。

集合B定义了下面的表1中描述的序列表的多肽序列以及相关位置的序列修饰。

在另一个实施方案中，分离的核酸分子长度至少为15个核苷酸并且在严紧条件下与含有集合A的核苷酸序列的核酸分子杂交。分离的核酸分子优选与天然存在的核酸分子对应。分离的核酸更优选编码天然存在的谷氨酸棒状杆菌MR蛋白或其生物活性部分。

本发明的另一个方面涉及包含本发明核酸分子的载体(例如重组表达载体)，还涉及已导入所述载体的宿主细胞。在一个实施方案中，通过使用在合适的培养基中培养的宿主细胞制备了MR蛋白。然后可从培养基或宿主细胞中分离该MR蛋白。

本发明的另一个方面涉及已导入MR基因的或其中MR基因被修饰的经遗传修饰的微生物。在一个实施方案中，通过导入至少一种作为转基因的编码突变MR序列的本发明核酸分子而修饰了所述微生物的基因组。在另一个实施方案中，通过用经修饰的MR基因进行同源重组而修饰(例如功能性破坏)了所述微生物基因组中的内源性MR基因。在一个优选的实施方案中，微生物属于棒状杆菌属或短杆菌(Brevibacterium)属，尤其优选谷氨酸棒状杆菌。在一个优选的实施方案中，该微生物也被用于制备目标化合物，如氨基酸、尤其是赖氨酸。

另一个优选的实施方案是具有一种或多种集合A中所描述核酸分子的宿主细胞。可用技术人员已知的多种方法制备这些宿主细胞。例如，可通过携带几种本发明核酸分子的载体转染这些细胞。然而，也可使用每次导入一种本发明核酸分子的载体从而同时或相继使用多种载体。从而可构建携带许多、高达数百的本发明核酸序列的宿主细胞。这种积累通常能够对宿主细胞精细化学品的生产率产生超加性效果。

本发明的另一方面涉及分离的MR蛋白或其部分，例如其生物活性部分。在一个优选的实施方案中，分离的MR蛋白或其部分在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的一种或多种代谢途径。在另一个优选的实施方案中，分离的MR蛋白和其部分与集合B的氨基酸序列足够同源，因为该蛋白或其部分仍然能够转录地、翻译地或翻译后地调节谷氨酸棒状杆菌中的一种或多种代谢途径。

此外，本发明涉及分离的MR蛋白制品。在优选的实施方案中，该MR蛋白含有集合B的氨基酸序列。在另一个优选的实施方案中，本发明涉及分离的全长蛋白，其和集合B的全长氨基酸序列(其由集合A中的可读框编码)充分同源。

可以将MR多肽或其生物活性部分与非MR多肽功能性地连接以产生融合蛋白。在优选的实施方案中，该融合蛋白具有不同于单独的MR蛋白的活性，在其它优选的实施方案中，该融合蛋白转录地、翻译地或翻译后地调节谷氨酸棒状杆菌的一种或多种代谢途径。在尤其优选的实施方案中，所述融合蛋白向宿主细胞的整合调节细胞对目的化合物的生产率。

本发明的另一个方面涉及制备精细化学品的制备方法。该方法涉及含有载体的细胞的培养，这导致本发明MR核酸分子的表达而产生精细化学品。在一个优选的实施方案中，该方法还包括得到含有此类载体的细胞的步骤，所述细胞用载体转染导致MR核酸表达。在另一个优选的实施方案中，所述方法还包括从培养物中得到精细化学品的步骤。在一个优选的实施方案中，该细胞属于棒状杆菌属或短杆菌属。

本发明的另一个方面涉及调节来自微生物的分子生产率的方法。这些方法包括将细胞与调节MR蛋白活性或MR核酸表达的物质接触，使得和没有所述物质存在时相比细胞相关的活性受到调节。在一个优选的实施方案中，细胞关于谷氨酸棒状杆菌中代谢途径的一种或多种调节系统受到调节，使得该微生物的目标精细化学品的产量或生产率提高。调节MR蛋白活性的物质刺激例如MR蛋白的活性或MR核酸的表达。刺激MR蛋白活性或MR核酸表达的物质的例子包括小分子、活性MR蛋白和编码MR蛋白并已导入细胞的核酸分子。抑制MR活性或MR表达的物质的例子包括小分子和反义MR核酸分子。

本发明的另一个方面涉及调节细胞的目的化合物产量的方法，该方法包括向细胞中导入MR野生型基因或MR突变基因，该基因仍位于分立的质粒上或者整合到宿主细胞的基因组中。向基因组的整合可随机发生或者通过同源重组发生，由此天然基因被整合的拷贝所代替，导致细胞的目标化合物的生产率被调节。在一个优选的实施方案中，所述产量增加了。在另一个优选的实施方案中，化学品是精细化学品，其在一个特别优选的实施方案中是氨基酸。在一个特别优选的实施方案中，该氨基酸是L-赖氨酸。

发明详述

本发明提供了参与谷氨酸棒状杆菌代谢调节，包括参与精细化学品代谢调节的MR核酸和MR蛋白分子。本发明的这些分子可用于直接调节(例如当对赖氨酸代谢途径的调节蛋白活性的调节对该微生物的赖氨酸产量、生产率和/或生产效率具有直接影响)或间接调节微生物如谷氨酸棒状杆菌的精细化学品的生产率，间接调节仍然增加目标化合物的产量、生产率和/或生产效率(例如，当对核苷酸生物合成蛋白的调节对细菌的有机酸或脂肪酸的生产率具有影响时，这可能是由于所述化学品的生物合成或降解途径中伴随的调节修饰对核苷酸生物合成的修饰调节的一种反应)。本发明的诸方面进一步阐述如下。

I.精细化学品

术语“精细化学品”为本领域所认知，其包括已用于多种工业的由生物体产生的分子，其中所述工业例如但不限于药学工业、农业工业和化妆品工业。此类化合物包括有机酸如酒石酸、衣康酸和二氨基庚二酸、蛋白原的和非蛋白原的氨基酸、嘌呤和嘧啶碱基、核苷和核苷酸(如在Rehm等人编辑的生物技术(Biotechnology)第6卷中Kuninaka，A.(1996)核苷酸和相关化合物(Nucleotides and related compounds)，561-612页，VCH：Weinheim及其中所含参考文献中所述)、脂类、饱和和不饱和脂肪酸(如花生四烯酸)、二醇(如丙二醇和丁二醇)、碳水化合物(如透明质酸和海藻糖)、芳香化合物(如芳香胺、香草醛和靛蓝)、维生素和辅因子(如在Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)，A27卷，“维生素”(“Vitamins”)，443-613页(1996)VCH：Weinheim及其中的参考文献；以及Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病”论文集(“Nutrition，Lipids，Health，and Disease”Proceedings ofthe UNESCO/Confederation of Scientific and Technological Associationsin Malaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press，(1995))、酶以及在Gutcho(1983)通过发酵的化学品(Chemicals by Fermentation)，Noyes DataCorporation，ISBN：0818805086和其中的参考文献中所述的所有其它化学品。这些精细化学品中的某些化学品的代谢和用途进一步阐述如下。

A.氨基酸代谢和用途

氨基酸包含所有蛋白质的基本结构单元，并且为所有生物体的正常细胞功能所必需。术语“氨基酸”为本领域所认知。蛋白原的氨基酸有20种，为蛋白质的结构单元，在蛋白质中它们通过肽键连接，非蛋白原的氨基酸(已知成百种非蛋白原的氨基酸)在蛋白质中通常不存在(参见Ulmann工业化学百科全书(Ulmann工业化学百科全书(Ulmann’s Encyclopediaof Industrial Chemistry))，A2卷，57-97页，VCH：Weinheim(1985))。氨基酸可为D-或L-构型，尽管在天然存在的蛋白质中发现的唯一类型通常为L-氨基酸。已充分说明了20种蛋白原氨基酸中每一种在原核和真核细胞中的生物合成和降解途径(参见例如，Stryer，L.生物化学(Biochemistry)，第三版，578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)，这样称呼是因为由于它们生物合成的复杂性，必须从食物中摄取它们，它们被简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物能够合成这些氨基酸中的一部分氨基酸，但必需氨基酸必须从食物中摄取，以进行正常的蛋白质合成。

除了它们在蛋白质生物合成中的功能外，这些氨基酸本身还是令人感兴趣的化学品，并且许多已发现在食品、动物饲料、化学品、化妆品、农业和药学工业中具有多种应用。赖氨酸不仅对人的营养而且对单胃动物如禽和猪都是一种重要的氨基酸。谷氨酸盐是最常用的调味添加剂(谷氨酸单钠盐，MSG)，并且广泛用于食品工业，天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸均用于药学工业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸用于药学工业和化妆品工业。苏氨酸、色氨酸和D/L-甲硫氨酸是广泛使用的动物饲料添加剂。(Rehm等人(编辑)，生物技术(Biotechnology)，第6卷，第14a章中的Leuchtenberger，W.(1996)Amino aids-technicalproduction and use，466-502页，VCH：Weinheim)。还发现这些氨基酸可用作前体，用于合成合成的氨基酸和蛋白质，如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟色氨以及在其他Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)，A2卷，57-97页，VCH：Weinheim，1985中所述的物质。

在可产生它们的生物体如细菌中已充分描述了这些天然氨基酸的生物合成(细菌氨基酸生物合成及其调节的综述，见Umbarger，H.E.(1978)Ann.Rev.Biochem.47：533-606)。谷氨酸通过还原胺化α-酮戊二酸(柠檬酸循环中的中间体)而合成。谷氨酰胺、脯氨酸和精氨酸接下来由谷氨酸产生。丝氨酸的生物合成为三步，由3-磷酸甘油(糖酵解中间体)开始，在氧化、氨基转移和水解步骤后产生该氨基酸。半胱氨酸和甘氨酸两者均产生自丝氨酸；在丝氨酸转羟甲基酶催化的反应中前者通过高半胱氨酸与丝氨酸缩合，后者通过侧链β-碳原子转移至四氢叶酸。苯丙氨酸和酪氨酸在9步的生物合成途径中合成自糖分解和磷酸戊糖途径的前体赤藓糖4-磷酸和磷酸烯醇丙酮酸，它们的不同仅在预苯酸合成后的最后两步。色氨酸也从这两种起始分子产生，但其合成为11步的途径。酪氨酸也可在苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸均为丙酮酸盐(糖酵解的终产物)的生物合成产物。天冬氨酸形成自草酰乙酸(柠檬酸循环的中间体)。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸均通过转化天冬氨酸而形成。异亮氨酸从苏氨酸形成。组氨酸在一个复杂的9步途径中从一种活化糖--5-磷酸核糖-1-焦磷酸形成。

为细胞蛋白质合成所需的过量氨基酸不能被贮藏，并被降解以提供用于细胞主要代谢途径的中间体(综述参见Stryer，L.生物化学(Biochemistry)第三版，21章，“氨基酸降解和尿素循环”495-516页(1988))。虽然细胞可将不想要的氨基酸转化为有用的代谢中间体，从所需用于合成氨基酸的能量、前体分子和酶来说，氨基酸的产生是昂贵的。因此对于氨基酸的生物合成受到反馈抑制调节并不让人吃惊，其中特定氨基酸的存在起到减缓或完全终止其自身产生的作用(关于氨基酸生物合成途径中的反馈机制的综述参见Stryer，L.生物化学(Biochemistry)第三版，24章：”氨基酸和血红素的生物合成”575-600页(1988))。因此，任一特定氨基酸的输出受到细胞中存在的该氨基酸量的限制。

B.维生素、辅因子和营养成分的代谢和用途

维生素、辅因子和营养成分包含高等动物不能合成且必须摄取的另一类分子，虽然它们易于被其它生物体如细菌合成。这些分子自身为生物活性物质或为在多种代谢途径中作为电子载体或中间体的生物活性物质的前体。除了它们的营养价值外，这些化合物作为着色剂、抗氧化剂和催化剂或其它操作辅助剂也具有重要的工业价值(这些化合物的结构、活性和工业应用的概述参见例如，Ullman工业化学百科全书(Ullman’s Encyclopediaof Industrial Chemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996)。术语”维生素”为本领域认知，包括生物体行使正常功能所需的营养素，且该生物体自身不能合成它们。维生素组和包含辅因子和营养性化合物。术语“辅因子”包括进行正常酶促活性所需的非蛋白原化合物。此类化合物可为有机的或无机的，本发明的辅因子优选有机的。术语“营养成分”包括促进植物和动物尤其是人的健康食物添加剂。此类分子的实例为维生素、抗氧化剂和某些脂类(如多不饱和脂肪酸)。

已大量描述了这些分子在可产生它们的生物体如细菌中的生物合成(Ullman工业化学百科全书(Ullman’s Encyclopedia of IndustrialChemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wiley&Sons；Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病”论文集(“Nutrition，Lipids，Health，and Disease”Proceedings of theUNESCO/Confederation of Scientific and Technological Associations inMalaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press：Champaign，IL X，374 S)。

硫胺素(维生素B₁)由嘧啶和噻唑部分通过化学偶联产生。核黄素(维生素B₂)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素接下来用于合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。合起来称为‘维生素B₆’的化合物类(例如，吡哆素、吡哆胺、5’-磷酸吡哆醛和商业上使用的维生素B₆盐酸盐)均为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸(R)-(+)-N-(2，4-二羟基-3，3-二甲基-1-氧代丁基)-β-丙氨酸)可通过化学合成或发酵产生。泛酸生物合成的最后步骤由ATP-驱动的β-丙氨酸和泛解酸的缩合组成。负责转化为泛解酸、β-丙氨酸和泛酸缩合生物合成步骤的酶是已知的。泛酸盐的代谢活性形式为辅酶A，其生物合成由5步酶促步骤进行。泛酸盐、5’-磷酸吡哆醛、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸盐的形成，而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(维生素B₅原)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。

已详细研究了微生物中生物素从前体分子庚二酰辅酶A的生物合成，并鉴定了几种所涉及的基因。发现许多相应的蛋白质也参与Fe-簇合成，属于nifS蛋白质类。硫辛酸衍生自辛酸，并在能量代谢中作为辅酶，此时其成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸盐为一组这样的物质，其均为叶酸衍生物，来自于L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已详细研究了某些微生物中始于生物转化代谢中间体鸟苷-5’-三磷酸(GTP)、L-谷氨酸和对氨基苯甲酸的叶酸及其衍生物的生物合成。

类可啉(如钴胺素且尤其是维生素B₁₂)和卟啉属于一组四吡咯环体系的化学品。维生素B₁₂的生物合成太复杂，目前尚未彻底解释清楚，但许多涉及的酶和物质已知。烟酸(烟酸盐)和烟酰胺为吡啶衍生物，也称为‘烟酸’。烟酸为重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)和它们的还原形式的前体。

这些化合物的大规模产生很大程度上依赖于无细胞的化学合成，虽然这些化学品中的一些如核黄素、维生素B₆、泛酸盐和生物素也已通过微生物的大规模培养物产生。只有维生素B₁₂是仅通过发酵产生的，因为其合成的复杂性。体外方法需要耗费材料和时间，通常以高的代价耗费。

C.嘌呤、嘧啶、核苷和核苷酸的代谢和用途

嘌呤和嘧啶代谢基因及它们相应的蛋白质为治疗肿瘤和病毒感染的重要靶标。术语“嘌呤”或“嘧啶”包括构成核酸、辅酶和核苷酸的含氮碱基。术语“核苷酸”包括核酸分子的基本结构单元，其由含氮碱基、戊糖(对RNA而言，该糖为核糖，对DNA而言，该糖为D-脱氧核糖)和磷酸组成。术语“核苷”包括作为核苷酸前体的分子，但其不含核苷酸所具有的磷酸单元。通过抑制这些分子的生物合成，或抑制它们形成核酸分子的代谢，可抑制RNA和DNA合成；通过以靶向癌细胞的方式抑制这种活性，可抑制肿瘤细胞分裂和复制的能力。

此外，有不形成核酸分子但作为能量贮存体(即AMP)或作为辅酶(即FAD和NAD)的核苷酸。

一些出版物已描述了这些化学品通过影响嘌呤和/或嘧啶代谢对这些医学适应症的用途(例如Christopherson，R.I.和Lyons，S.D.(1990)“作为化学治疗剂的嘧啶和嘌呤从头生物合成的强力抑制剂”医学研究述评(Med.Res.Reviews)10：505-548)。对参与嘌呤和嘧啶代谢的酶的研究集中在研发可用作如免疫抑制剂或抗增生剂的新药上(Smith，J.L.，(1995)“核苷酸合成中的酶”结构生物学最新观点(Curr.Opin.Struct.Biol.)5：752-757；(1995)Biochem Soc.Transact.23：877-902)。但是，嘌呤和嘧啶碱基、核苷和核苷酸具有其它应用：用作一些精细化学品(例如，硫胺素、S-腺苷-甲硫氨酸、叶酸盐或核黄素)生物合成的中间体，作为细胞的能量载体(例如，ATP或GTP)，和本身作为化学品，通常作为增味剂(例如，IMP或GMP)或用于一些医药用途(参见例如，Kuninaka，A.(1996)生物技术中的核苷酸和相关化合物(Nucleotides and Related Compounds inBiotechnology)第6卷，Rehm等人编辑.VCH：Weinheim，561-612页)。同样，参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作靶，研发用于作物保护的抗这些酶的化学品包括杀真菌剂、除草剂和杀虫剂。

已阐释了这些化合物在细菌中的代谢(综述参见例如，核酸研究和分子生物学进展(Progress in Nucleic Acid Research and Molecular Biology)，第42卷，Academic Press中的Zalkin，H.和Dixon，J.E.(1992)“嘌呤核苷酸的从头生物合成”，259-287页；和生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：纽约一书中的Michal，G.(1999)“核苷酸和核苷”，第8章)。嘌呤代谢已是深入研究的主题，其对细胞的正常功能是必需的。高等动物中受损的嘌呤代谢可引起严重疾病，如痛风。嘌呤核苷酸从核糖-5-磷酸开始，经过一系列步骤，通过中间体化合物次黄苷-5’-磷酸(IMP)，产生鸟苷-5’-单磷酸(GMP)或腺苷-5’-单磷酸(AMP)，由此易于形成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存体，这样它们的降解可提供细胞内用于许多不同生物化学过程的能量。嘧啶的生物合成由核糖-5-磷酸形成尿苷-5’-单磷酸(UMP)而进行。UMP接下来转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式通过一步还原步骤从核苷酸的二磷酸核糖形式产生为核苷酸的二磷酸脱氧核糖形式。通过磷酸化后，这些分子可参与DNA合成。

D.海藻糖的代谢和用途

海藻糖由两个葡萄糖分子通过α，α-1，1连接组成。它通常作为甜味剂用于食品工业，为干的或冷冻食品和饮料中的添加剂。但它也用于药学、化妆品和生物技术工业(参见例如，Nishimoto等人(1998)美国专利号5,759,610；Singer，M.A.和Lindquist，S.生物技术趋势(Trends Biotech.)16(1998)：460-467；Paiva，C.L.A.和Panek，A.D.Biotech.Ann.Rev.2(1996)：293-314；和Shiosaka，M.J.Japan 172：(1997)97-102)。海藻糖通过酶从许多微生物产生，并天然释放入环境介质中，从中可用本领域公知的方法收集海藻糖。

II.代谢调节机制

所有活细胞都有复杂的分解代谢和合成代谢能力，其中许多代谢途径相互关联。为了维持该异常复杂的代谢网络的各部分之间的平衡，细胞采用了精密调节的调节系统。通过独立地或同时地调节酶合成和酶活性，细胞能够调节完全不同的代谢途径的活性以便满足细胞不断变化的需要。

可以在转录水平或翻译水平或者这两种水平上诱导或者抑制酶的合成(综述见Lewin，B.(1990)基因IV(Genes IV)，第3部分：“通过转录调控原核细胞基因(Controlling prokaryotic genes by transcription)”，牛津大学出版社，牛津，213-301页，及其参考文献，和Michal，G.(1999)，生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas ofBiochemistry and Molecular Biology)，John Wiley&Sons)。所有这些已知的调节过程都被应答多种外部影响(例如温度、营养供给或光)的其他基因介导。参与这种类型调节的蛋白因子的例子包括转录因子。它们是结合DNA从而导致基因表达或者增加(正调节，如大肠杆菌ara操纵子)或者降低(负调节，如大肠杆菌lac操纵子)的蛋白质。这些调节表达的转录因子本身可受到调节。例如，它们的活性可受到结合DNA结合蛋白的低分子量化合物的调节，从而所述蛋白对DNA上合适的结合位点的结合被刺激(如ara操纵子的阿拉伯糖)或抑制(如lac操纵子的乳糖)(见，例如，Helmann，J.D.和Chamberlin，M.J.(1988)，“细菌σ因子的结构和功能(Structure and function of bacterial sigma factors)”Ann.Rev.Biochem.57：839-872；Adhya，S.(1995)“今日lac和gal操纵子(The lac and galoperons today)”以及Boos，W.等人，“麦芽糖体系(The maltose system)”，这两篇文章在大肠杆菌中基因表达的调节(Regulation of Gene Expressionin Escherichia coli)一书中，由Lin，E.C.C.和Lynch，A.S.编辑，Chapman&Hall：New York，181-200页和201-229页；和Moran，C.P.(1993)“RNA聚合酶和转录因子(RNA polymerase and transcription factors)”，在：枯草芽孢杆菌和其他革兰氏阳性菌(Bacillus subtilis and other Gram-positivebacteria)一书中，由Sonenshein，A.L.编辑ASM：Washington D.C.653-667页)。

蛋白质的合成不仅在转录水平而且在翻译水平受到调节。可以通过许多机制进行调节，包括对核糖体结合一种或多种mRNA能力的修饰、核糖体与mRNA的结合、保持或去除mRNA二级结构、对特定基因使用常见或不常见密码子、一种或多种tRNA的丰度和特定调节机制如弱化作用进行调节(见Vellanoweth，R.I.(1993)枯草芽孢杆菌和其他革兰氏阳性菌中的翻译及其调节(Translation and its regulation in Bacillus subtilis andother Gram-positive bacteria)，Sonenshein，A.L.等人编辑ASM：Washington，D.C.，699-711页及其参考文献)。

可以对各种代谢途径中的单个蛋白(相继调节)或同时对多种蛋白(协同调节)进行转录和翻译调节。表达受到协同方式调节的基因通常位于基因组中操纵子或调节子接近处。这种基因转录和基因翻译的上调或下调受到多种因素如底物(在一种或多种代谢途径中使用的前体和中间体)、分解代谢物(与从复杂有机分子如糖的降解的能量产生相关的生物化学途径产生的分子)和终产物(在代谢途径末得到的分子)的细胞或细胞外的量控制。编码特定代谢途径的活性所需的酶的基因表达被所述代谢途径的大量底物分子所诱导。相应地，该基因表达被胞内大量该途径的终产物所抑制(Snyder，L.和Champness，W.(1977)，细菌的分子生物学(TheMolecular Biology of Bacteria)ASM：Washington)。基因表达同样可被其他外部或内部因素如环境条件(例如热、氧化性应急或饥饿)所调节。这种全局环境变化导致专门的调节基因表达的变化，这些调节基因通过结合DNA而直接或间接(通过其他基因或蛋白)引发基因表达，从而诱导或抑制转录(见，例如，Lin，E.C.C.和Lynch，A.S.编辑(1995)，大肠杆菌中基因表达的调节(Regulation of Gene Expression in Escherichia coli)，Chapman&Hall：New York)。

调节细胞代谢的另一个机制发生在蛋白质水平。该调节通过其他酶的活性或通过结合阻止或赋予该蛋白正常功能的低分子量组分进行。通过结合低分子量化合物进行蛋白调节的例子包括GTP或NAD的结合。低分子量化学物质的结合通常是可逆的，例如GTP结合蛋白。这些蛋白存在两种状态(结合GTP或GDP)，一种状态是蛋白质的活性形式，另一中是无活性形式。

蛋白活性被其他酶的作用调节，其中其他酶通常通过对蛋白的共价修饰(即氨基酸残基如组氨酸或天冬氨酸的磷酸化或甲基化)调节。这种共价修饰通常是可逆的并且受到具有相反活性的酶影响。一个例子是激酶和磷酸酶在蛋白质磷酸化中的相反活性：蛋白激酶在靶蛋白上(例如丝氨酸或苏氨酸)磷酸化特定残基，而蛋白磷酸酶从所述蛋白除去磷酸基团。调节其他蛋白活性的酶本身通常受到外界刺激的调节。这些刺激被作为传感器的蛋白所介导。被这些传感器蛋白介导外界信号的一个熟知的机制是二聚体化，但也知道其他机制(见，例如Msadek，T.等(1993)“双组分调节体系(Two-component Regulatory Systems)”在：枯草芽孢杆菌和其他革兰氏阳性菌(Bacillus subtilis and Other Gram-Positive Bacteria)一书中，Sonenshein，A.L.等人编辑，ASM：Washington，729-745页及其参考文献)。

详细了解调控微生物中细胞代谢的调节网络对通过发酵高产量地生产化学品是关键的。可以去除或降低下调代谢途径的调控系统以便提高目标化学品的合成，相应地，可以组成性地活化或优化上调目标产物的代谢途径的调控系统的活性(如Hirose，Y.和Okada，H.(1979)“氨基酸的微生物生产(Microbial Production of Amino Acids)”，在：Peppler，H.J.和Perlman，D.(编辑)微生物技术(Microbial Technology)第二版，第1卷，第7章，Academic Press，New York)。

III.本发明的组成要件和方法

本发明至少部分是基于本文中称作MR核酸和MR蛋白分子的新分子的检测，这些分子通过转录、翻译或翻译后的措施调节谷氨酸棒状杆菌中的一种或多种代谢途径。在一个实施方案中，MR分子转录地、翻译地或翻译后地调节谷氨酸棒状杆菌中的代谢途径。在一个优选的实施方案中，调节谷氨酸棒状杆菌中的一种或多种代谢途径的本发明MR分子的活性影响所述生物体的目标精细化学品的生产率。在一个尤其优选的实施方案中，本发明的MR分子具有经调节的活性，使得被本发明MR蛋白调节的谷氨酸棒状杆菌代谢途径的效率或产量受到调节，并且这直接或间接地调节谷氨酸棒状杆菌的目标精细化学品的产量、生产率和/或生产效率。

术语“MR蛋白”或“MR多肽”包括转录中、翻译中或者翻译后调节谷氨酸棒状杆菌中代谢途径的蛋白。MR蛋白的例子包括表1和集合A中所列出的MR基因编码的那些蛋白。术语“MR基因”和“MR核酸序列”包括编码MR蛋白的核酸序列，其含有一个编码区和相应的非翻译5’和3’序列区。MR基因的例子在表1中列出。术语“生产率”为本领域已知并且包括发酵产物的浓度(例如在预定的时间段和预定的发酵体积产生的目标精细化学品(例如kg产物/h/l))的浓度。术语“生产效率”包括得到特定产物量所需时间(例如，细胞达到精细化学品的特定生产率所需时间)。术语“产量”或“产物/碳产量”为本领域已知并且包括将碳源转化成产物(即精细化学品)的效率。这例如通常表达为kg产物/kg碳源。增加化合物的产量或生产率为在预定期间内及特定的培养体积中增加了所得分子的量或该化合物适宜得到的分子的量。术语“生物合成”和“生物合成途径”为本领域已知并且包括细胞在例如多步过程或高度调节的过程中从中间物合成化合物，优选有机化合物。术语“降解”和“降解途径”为本领域已知并且包括细胞在例如多步过程或高度调节的过程中将化合物，优选有机化合物裂解成降解产物(用更通俗的术语：更小或更简单的分子)。术语“代谢”为本领域已知并且包括生物体中发生的全部生物化学反应。特定化合物的代谢(例如氨基酸如甘氨酸的代谢)因此包括细胞中该化合物的所有生物合成、修饰和降解途径。术语“调节”为本领域已知并且包括调控另一蛋白质活性的蛋白质的活性。术语“转录调节”为本领域已知并且包括抑制或活化编码靶蛋白的DNA转化成mRNA的蛋白质的活性。术语“翻译调节”为本领域已知并且包括抑制或活化编码靶蛋白的mRNA转化成蛋白分子的蛋白质的活性。术语“翻译后调节”为本领域已知并且包括通过共价修饰靶蛋白(例如通过甲基化、糖基化或磷酸化)抑制或提高靶蛋白活性的蛋白质的活性。

在另一个实施方案中，微生物如谷氨酸棒状杆菌中本发明的MR分子能够调节目标分子例如精细化学品的生产率。借助于基因重组技术，可操作一种或多种本发明的代谢途径调节蛋白使得它们的功能被调节。可以例如提高生物合成酶的效率或者破坏其变构调控区以便阻止该化合物产生的反馈抑制。所以，可以通过替换、缺失或添加来删除或修饰降解酶使得其对目标化合物的降解活性降低而不损害细胞的存活。在任何情况下，可以增加任何所述目标精细化学品的总产量或生产率。

还可能的是本发明的蛋白和核苷酸分子的这些修饰能够间接提高精细化学品的生产率。细胞中代谢途径的调节机制不可避免地相关联，一种代谢途径的激活能够伴随着抑制或激活另一种代谢途径。调节本发明的一种或多种蛋白的活性能影响其他精细化学品生物合成或降解途径活性的生产率或效率。降低一种MR蛋白抑制编码氨基酸生物合成中特定蛋白的基因转录使得可能同时抑制其他氨基酸生物合成途径，因为这些代谢途径相互关联。通过调节本发明MR蛋白可能使细胞生长和细胞分裂与细胞胞外环境以一定程度解偶联；当用于生长和细胞分裂的胞外条件不是最佳时某种MR蛋白通常抑制核苷酸的生物合成，通过影响该MR蛋白使其失去抑制核苷酸合成的功能，可能使得即使胞外条件较差时仍能生长。这对于大规模发酵培养尤其重要，大规模发酵的温度、营养供给或通气通常不是最佳的，但是当所述因子的细胞调节系统被去除后仍然能够促进生长和细胞分裂。

制备本发明核酸序列的一个适宜的起点是谷氨酸棒状杆菌菌株的基因组，可以从美国典型培养物保藏中心得到该菌株(名称是ATCC 13032)。

用常规方法，通过表1所示的修饰可以从这些核酸序列制备本发明的核酸序列。

本发明的MR蛋白或其生物学活性部分或片段能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径或可以具有表1列出的一种或多种活性。

下面的分段详细描述本发明的各方面：

A.分离的核酸分子

本发明的一个方面涉及编码MR多肽或其生物活性部分的分离核酸分子及核酸分子片段，其中所述核酸分子片段足以用作杂交探针或引物，用来鉴定或扩增编码MR的核酸(例如MR DNA)。如此处所用，术语“核酸分子”是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)和用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’和5’端的非翻译区：基因编码区5’端上游的至少约100个核苷酸序列和基因编码区3’端下游的至少约20个核苷酸序列。核酸分子可为单链或双链，但优选地为双链DNA。“分离的”核酸分子为从其它核酸分子分离的核酸分子，其存在于核酸的天然源中。优选地，“分离的”核酸不具有该核酸所来自的生物体基因组DNA中天然存在于该核酸侧翼的序列(即位于该核酸5’和3’端的序列)。例如，在不同实施方案中，该分离的MR核酸分子可包含少于约5kb，4kb，3kb，2kb，1kb，0.5kb或0.1kb的该核酸所来自的细胞(如谷氨酸棒状杆菌细胞)基因组DNA中天然存在于该核酸分子侧翼的核苷酸序列。而且，“分离的”核酸分子如cDNA分子当通过重组技术产生时可基本上不含有其它细胞物质或培养基，当通过化学合成产生时，可基本上不含有化学前体或其它化学品。

本发明的核酸分子，如具有的核苷酸序列为集合A的核酸分子或其部分可用标准的分子生物学技术和本文提供的序列信息制备。例如，使用集合A全部或部分序列作为杂交探针和标准的杂交技术(例如，如在Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，冷泉港实验室，冷泉港实验室出版社，冷泉港，纽约，1989中所述)，可自谷氨酸棒状杆菌文库分离谷氨酸棒状杆菌MR DNA。而且，包含集合A的全部序列或其部分的核酸分子可使用根据该序列设计的寡核苷酸引物，应用聚合酶链反应分离(例如，包含集合A的全部或部分的核酸分子可使用根据该同样序列设计的寡核苷酸引物，应用聚合酶链反应分离)。例如，可从正常的内皮细胞分离mRNA(例如，通过Chirgwin等人(1979)生物化学(Biochemistry)18：5294-5299的硫氰酸胍提取法)以及用反转录酶(例如，莫洛尼鼠类白血病毒反转录酶，可从Gibco/BRL，Bethesda，MD获得；或AMV反转录酶，可从Seikagaku America，Inc.，St.Petersburg，FL获得)可制备cDNA。用于聚合酶链反应扩增的合成寡核苷酸引物可根据集合A所示的核苷酸序列之一设计。本发明的核酸可用cDNA或另外用基因组DNA作为模板、使用合适的寡核苷酸引物按照标准的PCR扩增技术来扩增。这样扩增的核酸可克隆入合适的载体并通过DNA序列分析鉴定。对应于MR核苷酸序列的寡核苷酸可通过标准的合成技术制备，例如用DNA自动合成仪制备。

在一个优选的实施方案中，本发明的分离核酸分子包含集合A中所示的任一核苷酸序列。

在另一个优选的实施方案中，本发明的分离的核酸分子含有与集合A中所示核酸序列之一互补的核酸分子，所述核酸分子与集合A中所示核酸序列之一足够互补使其与集合A中所示序列之一杂交而得到稳定双链体。

在一个实施方案中，本发明的核酸分子编码这样的蛋白质或其部分，其包括的氨基酸序列充分同源于集合B的一个氨基酸序列，以至于该蛋白质或其部分仍然能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径。如此处所用，术语“充分同源”是指蛋白质或其部分的氨基酸序列具有最小数的与集合B中氨基酸序列相同或等同的氨基酸残基(例如，氨基酸残基具有与集合B中序列之一的氨基酸残基相似的侧链)，以至于该蛋白质或其部分能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径。此类代谢途径中的蛋白质成员如此处所述，可调节一种或多种精细化学品的生物合成或降解。此类活性的实例也在此进行了描述。因此，“MR蛋白的功能”涉及一种或多种精细化学品代谢途径的总体调节。MR蛋白活性的实例在表1中给出。

本发明MR核酸分子编码的蛋白部分优选地是任何MR蛋白的具生物学活性的部分。文中所用术语“MR蛋白的生物学活性部分”包含MR蛋白的一部分，例如结构域或基序，其能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径或者具有表1中所示活性。为了确定MR蛋白或者其生物学活性部分能否在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径，可以进行酶活性分析。这些分析方法在实施例部分的实施例8中详细描述，技术人员熟知这些方法。

除了群体中存在的MR序列的天然变体外，技术人员还理解可通过突变向集合A的核苷酸序列中导入变化，由此导致所编码的MR蛋白的氨基酸序列改变，但MR蛋白的功能不变。例如，可在集合A的核苷酸序列中进行在“非必需”氨基酸残基处导致氨基酸替换的核苷酸替换。可以修饰任一MR蛋白(集合B)的野生型序列中的“非必需”氨基酸残基而不改变所述MR蛋白的活性，但“必需”氨基酸残基为MR蛋白活性所需。然而，其它氨基酸残基(例如，在具有MR活性的结构域中不保守或仅为半保守的那些氨基酸残基)对活性可为非必需的，并因此可被改变，而此类氨基酸残基的改变不改变MR活性。

编码同源于集合B的蛋白质序列的MR蛋白的分离核酸分子可通过将一个或多个核苷酸替换、添加或删除导入集合A的核苷酸序列而产生，这样将一个或多个氨基酸的替换、添加或删除导入所编码的蛋白质。可通过标准技术将突变导入集合A的核苷酸序列之一，如定点诱变和PCR-介导的诱变。优选地，在一处或多处预测的非必需氨基酸残基处进行保守氨基酸替换。“保守氨基酸替换”为将氨基酸残基用具有相似侧链的氨基酸残基替换。具有相似侧链的氨基酸残基类已在本领域定义过。这些分类包括具有碱性侧链的氨基酸(例如，赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如，天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如，甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如，丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如，苏氨酸、缬氨酸、异亮氨酸)和具有芳香侧链的氨基酸(例如，酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此，在MR蛋白中预测的非必需氨基酸残基优选地用来自同一侧链类的另一氨基酸残基替换。在另一实施方案中，可如通过饱和诱变沿着全部或部分MR编码序列随机导入突变，并对所得突变体筛选文中所述的MR活性，以鉴定保留MR活性的突变体。对任一集合A的序列诱变后，可重组表达编码蛋白，并用例如文中所述的试验(参见实施例部分的实施例8)确定所述蛋白质的活性。

B.重组表达载体和宿主细胞

本发明的另一方面涉及包含编码MR蛋白(或其部分)的核酸或包含基因组合且其中至少一种基因编码MR蛋白的载体，优选表达载体。如此处所用，术语“载体”是指这样的核酸分子，其可转运与其连接的另一核酸。一种类型的载体为“质粒”，是指其中可连接额外DNA片段的环状双链DNA环。另一种类型的载体为病毒载体，其中可将额外DNA片段连接入病毒基因组。某些载体可在它们所导入的宿主细胞中自主复制(例如，具有细菌复制起点的细菌载体和游离型哺乳动物载体)。其它载体(例如，非游离型哺乳动物载体)在导入宿主细胞后整合入宿主细胞的基因组，并因此随着宿主基因组而复制。而且，某些载体可指导与它们操作性连接的基因的表达。此类载体此处称为“表达载体”。一般，用于重组DNA技术中的表达载体通常为质粒形式。在本说明书中，“质粒”和“载体”可互换使用，因为质粒是最经常使用的载体形式。但是，本发明旨在包括其它形式的表达载体，如病毒载体(例如，复制缺陷型反转录病毒、腺病毒和腺相关病毒)，它们起到类似的作用。

本发明的重组表达载体以适于在宿主细胞中表达核酸的形式包含本发明的核酸，这就是说重组表达载体包括一个或多个调节序列，其选择是基于用作表达的宿主细胞进行的，它们可操作地与待表达的核酸序列连接。在重组表达载体中，术语“功能性地连接”是指目的核苷酸序列与调节序列以允许该核苷酸序列表达(例如，在体外转录/翻译系统中，或当载体导入宿主细胞时在宿主细胞中表达)的方式连接。术语“调节序列”是指包括启动子、增强子和其它表达调控元件(例如，多聚腺苷酸化信号)。此类调节序列如在Goeddel；基因表达技术：酶学方法(GeneExpressionTechnology：Methods in Enzymology)185，Academic Press，SanDiego，CA(1990)中描述。调节序列包括在许多类型宿主细胞中控制核苷酸序列组成型表达的调节序列和仅在某些宿主细胞中控制核苷酸序列直接表达的调节序列。本领域普通技术人员了解到表达载体的设计可取决于如对欲转化宿主细胞的选择、所需的蛋白质表达程度等因素。本发明的表达载体可导入宿主细胞，以产生由此处所述核酸所编码的蛋白质或肽，包括融合蛋白或融合肽(例如，MR蛋白、MR蛋白的突变形式、融合蛋白等)。

本发明的重组表达载体可设计用于在原核或真核细胞中表达MR蛋白。例如，MR基因可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母细胞和其它真菌细胞(参见Romanos，M.A.等人(1992)“在酵母中外源基因的表达：综述”(“Foreign gene Expression in yeast：areview”)，酵母(Yeast)8：423-488；J.W.Bennet&L.L.Lasure编辑的更多的用于真菌的基因操作(More Gene Manipulations in Fungi)，AcademicPress：San Diego一书中的van den Hondel，C.A.M.J.J.等人(1991)“丝状真菌中的异源基因表达”(“Heterologous gene Expression in filamentousfungi”)，396-428页；以及Peberdy，J.F.等人编辑的真菌的应用分子遗传学(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(Gene transfer systems and vectordevelopment for filamentous fungi)，1-28页)、藻类和单细胞植物细胞(参见Schmidt，R.和Willmitzer，L.(1988)，根瘤农杆菌介导的在鼠耳芥叶和子叶外植体中的高效转化，植物细胞报道(Plant Cell Rep.)：583-586)、或哺乳动物细胞中表达。合适的宿主细胞在Goeddel，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，AcademicPress，San Diego，CA(1990)中进一步进行了讨论。另外，可体外转录并翻译重组表达载体，如使用T7启动子调节序列和T7聚合酶。

在原核生物中蛋白质的表达主要用含有组成型或诱导型启动子的载体进行，控制融合或非融合蛋白的表达。融合载体将一些氨基酸添加至其中编码的蛋白质上，通常添加至重组蛋白的氨基末端。此类融合载体一般具有三个任务：1)用于增强重组蛋白的表达；2)用于增加重组蛋白的溶解性；和3)在亲和层析中作为配体而利于重组蛋白的纯化。通常，于融合部分和重组蛋白的连接处导入蛋白酶剪切位点到融合表达载体中，这使得在该融合蛋白纯化后，可将重组蛋白从融合单元分离。这些酶和它们的对应识别序列包括Xa因子、凝血酶和肠激酶。

通常的融合表达载体包括pGEX(Pharmacia Biotech Inc；Smith，D.B.和Johnson，K.S.(1988)基因(Gene)67：31-40)、pMAL(New EnglandBiolabs，Beverly，MA)和pRIT5(Pharmacia，Piscataway，NJ)，它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白A结合至重组靶蛋白。在一个实施方案中，将MR蛋白的编码序列克隆入pGEX表达载体，以产生编码融合蛋白的载体，其从N-末端至C-末端包含GST-凝血酶切割位点-X蛋白。该融合蛋白使用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。通过将该融合蛋白用凝血酶切割可得到不与GST融合的重组MR蛋白。

合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等人(1988)基因(Gene)69：301-315)和pET 11d(Studier等人，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)60-89)。pTrc载体中目标基因的表达依赖于宿主RNA聚合酶从杂合的trp-lac融合启动子的转录。pET 11d载体中目标基因的表达依赖于共表达的病毒RNA聚合酶(T7 gn1)介导的从T7-gn10-lac融合启动子的转录。该病毒聚合酶由宿主菌株BL21(DE3)或HMS174(DE3)中居住的、具有位于lacUV 5启动子转录调控下的T7 gn1基因的λ前噬菌体提供。

最大化重组蛋白表达的一个策略是将该蛋白质在具有对所述重组蛋白进行蛋白酶切割的能力缺损的宿主细菌中表达(Gottesman，S.，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)119-128)。另一策略是改变欲插入表达载体的核酸的核酸序列，以使用于每一氨基酸的单个密码子为选择用于表达的细菌如谷氨酸棒状杆菌所优选使用的密码子(Wada等人(1992)核酸研究(Nucleic Acids Res.)20：2111-2118)。本发明核酸序列的此改变可应用标准的DNA合成技术实施。

在另一实施方案中，MR蛋白表达载体为酵母表达载体。用于在酿酒酵母中表达的载体包括pYepSec1(Baldari，等人(1987)Embo J.6：229-234)，pMFa(Kurjan和Herskowitz，(1982)细胞(Cell)30：933-943)，pJRY88(Schultz等人(1987)基因(Gene)54：113-123)，和pYES2(InvitrogenCorporation，San Diego，CA)。适用于其它真菌如丝状真菌的载体和用于构建载体的方法包括在：J.F.Peberdy等人编辑的真菌的应用分子遗传学(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(Gene transfer systems and vectordevelopment for filamentous fungi)，1-28页中详述的那些。

另外，本发明的MR蛋白可使用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf 9细胞)中表达蛋白质的杆状病毒载体包括pAc系列(Smith等人(1983)Mol.Cell Biol.3：2156-2165)和pVL系列(Lucklow和Summers(1989)病毒学(Virology)170：31-39)。

在另一实施方案中，本发明的MR蛋白可在单细胞植物细胞(如藻类)或在来自高等植物(如种子植物，诸如作物)的植物细胞中表达。植物表达载体的实例包括在：Becker，D.，KeMRer，E.，Schell，J.和Masterson，R.(1992)“具有靠近左边界的选择性标记的新植物双元载体(“New plantbinary vectors with selectable markers located proximal to the leftborder”)，植物分子生物学(Plant Mol.Biol.)20：1195-1197；和Bevan，M.W.(1984)“用于植物转化的双元农杆菌载体”(“Binary Agrobacteriumvectors for plant transformation”)，核酸研究(Nucl.Acid.Res.)12：8711-8721中详述的那些。

在另一实施方案中，本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed，B.(1987)自然(Nature)329：840)和pMT2PC(Kaufman等人(1987)EMBO J.6：187-195)。当用于哺乳动物细胞时，表达载体的调控功能通常由病毒调控元件提供。例如，常用的启动子来自多瘤、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它合适表达系统参见Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：ALaboratory Manual)，第二版，Cold Spring Harbor Laboratory，ColdSpring Harbor Laboratory Press，Cold Spring Harbor，NY，1989中的第16和17章。

在另一实施方案中，重组哺乳动物表达载体可优选地在特定细胞类型中指导核酸的表达(例如，使用组织-特异性调控元件来表达核酸)。组织-特异性调控元件为本领域公知。合适的组织-特异性启动子的非限制性实例包括白蛋白启动子(肝-特异性；Pinkert等人(1987)基因进展(Genes Dev.)1：268-277)、淋巴-特异性启动子(Calame and Eaton(1988)Adv.Immunol.43：235-275)，特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8：729-733)和免疫球蛋白启动子(Banerji等人(1983)细胞(Cell)33：729-740；Queen和Baltimore(1983)细胞(Cell)33：741-748)、神经元-特异性启动子(例如，神经丝启动子；Byrne和Ruddle(1989)PNAS86：5473-5477)、胰-特异性启动子(Edlund等人(1985)科学(Science)230：912-916)、以及乳腺-特异性启动子(例如，奶乳清启动子；美国专利号4,873,316和欧洲申请公开号264,166)。也同样包括调节发育的启动子，如鼠hox启动子(Kessel和Gruss(1990)科学(Science)249：374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)基因进展(Genes Dev.)3：537-546)。

本发明还提供了含本发明的DNA分子的重组表达载体，其中该DNA分子以反义方向克隆在表达载体中。也就是，DNA分子功能性地连接到调控序列上，该连接方式使得可以表达产生与MR mRNA反义的RNA分子(通过转录该DNA分子)。可以对功能性地连接反义方向克隆的核酸的调控序列进行选择以控制反义RNA分子在多种细胞类型中持续表达，例如，可选择病毒启动子和/或增强子或调控序列以控制反义RNA实现组成型组织特异性或细胞类型特异性表达。反义表达载体的形式可为重组质粒、噬菌粒或减毒病毒，其中在高效调控区域的控制下产生反义核酸，其活性由载体所导入细胞的类型决定。反义基因的基因表达调控的讨论见Weintraub，H.等人，反义RNA作为遗传分析的分子工具，Reviews-Trendsin Genetics，卷1(1)1986。

本发明的另一方面涉及已导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此处可互换使用。当然，该术语不仅指特定的受试细胞，也指该细胞的后代或潜在后代。由于突变或环境因素在传代过程中可发生某些改变，这样，后代实际上可能并非与亲代细胞相同，但其仍包括在此处所用术语的范围之内。

宿主细胞可为任意的原核或真核细胞。例如，MR蛋白可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢(CHO)细胞或COS细胞)中表达。其他合适的宿主细胞为技术人员公知。与谷氨酸棒状杆菌有关的、可常规用作本发明核酸和蛋白质分子的宿主细胞的微生物如表3所示。

载体DNA可通过常规转化或转染技术导入原核或真核细胞中。如此处所用，术语“转化”和“转染”意指本领域内公知的各种将外源核酸(例如DNA)导入宿主细胞的技术，包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖-介导的转染、脂转染、电穿孔。转化或转染宿主细胞的合适方法可在Sambrook等人(分子克隆：实验室手册(Molecular Cloning：A LaboratoryManual).第二版，Cold Spring Harbor Laboratory，Cold Spring HarborLaboratory Press，Cold Spring Harbor，NY，1989)和其它的实验室手册中找到。

对于哺乳动物细胞的稳定转染，已知根据所用的表达载体和所用的转染技术，仅一小部分的细胞可将外源DNA整合到基因组中。一般将编码选择标记的基因(如抗生素抗性基因)与目的基因一同导入宿主细胞以鉴定和筛选整合体。优选的选择标记包括那些可赋予药物抗性的基因，所述药物如G418、潮霉素和氨甲蝶呤。编码选择标记的核酸可与编码MR蛋白质的基因位于同一载体上被导入宿主细胞或可以位于不同的载体上导入。经导入核酸稳定转染的细胞可通过药物筛选进行鉴定(例如，掺入了选择标志基因的细胞将存活，而其他细胞死亡)。

通过制备出含至少部分MR基因的载体，所述部分MR基因中已经导入缺失、添加或替代因而可以改变MR基因(如功能性破坏)来生成同源重组微生物。优选地该MR基因为谷氨酸棒状杆菌MR基因，但也可为来自相关细菌或甚至来自哺乳动物、酵母或昆虫的同系物。在一个优选的实施方案中，载体设计为在同源重组后可功能性破坏内源MR基因(即，不再编码功能蛋白质；也称为“敲除”载体)。另外，载体可设计为在同源重组后内源MR基因发生突变或改变但仍编码功能蛋白质(例如，可改变上游调控区域由此改变内源MR蛋白的表达)。在此同源重组载体中，改变的MR基因部分在其5′和3′端侧翼为MR基因的其它核酸，从而允许在载体携带的外源MR基因和微生物中的内源MR基因之间发生同源重组。此其它的两翼MR核酸的长度应足以使与内源基因的重组成功进行。通常，载体内包括几千碱基的侧翼DNA(5′及3′端)(参见例如，Thomas，K.R.，和Capecchi，M.R.(1987)细胞(Cell)51：503中对同源重组载体的描述)。使用本领域公知的方法，将该载体导入微生物(例如，通过电穿孔)和细胞，筛选其中导入的MR基因已与内源MR基因同源重组的微生物和细胞。

在另一实施方案中，产生的重组微生物可含有可调控导入基因表达的所选系统。例如，将在载体中包含的MR基因插入乳糖操纵子的控制下，使得MR基因仅在存在IPTG时表达。此类调控系统为本领域熟知。

本发明的宿主细胞如培养的原核或真核宿主细胞可用于产生(即表达)MR蛋白。因此，本发明还提供了使用本发明的宿主细胞来产生MR蛋白的方法。在一个实施方案中，该方法包括在合适的培养基中培养本发明的宿主细胞(其中已导入了编码MR蛋白的重组表达载体，或其中基因组已导入了编码野生型或改变的MR蛋白的基因)，直至产生MR蛋白。在另一实施方案中，该方法还包括从培养基或宿主细胞分离MR蛋白。

C.本发明的用途和方法

文中所述的核酸分子、蛋白质、蛋白质同系物、融合蛋白、引物、载体和宿主细胞可用于一种或多种下述方法中：鉴定谷氨酸棒状杆菌及相关生物体；绘制与谷氨酸棒状杆菌相关的生物体的基因组图谱；鉴定并定位目标谷氨酸棒状杆菌序列；进化研究；确定为MR蛋白所需的功能区域；调节MR蛋白活性；调节MR途径的活性；以及调节目标化合物如精细化学品的细胞生产率。本发明的MR核酸分子具有多种用途。首先，它们可用于鉴定生物体为谷氨酸棒状杆菌或其近亲。它们也可用于在混合的微生物群体中鉴定谷氨酸棒状杆菌或其相关菌的存在。本发明提供了许多谷氨酸棒状杆菌基因的核酸序列。通过将微生物单一群体或混合群体的培养物提取的基因组DNA在严格条件下与包含对谷氨酸棒状杆菌独特的谷氨酸棒状杆菌基因区域的探针杂交，可确定该生物体是否存在。虽然谷氨酸棒状杆菌自身为非致病的，但它与致病种如白喉棒状杆菌有亲缘关系。这种生物体的检测具有显著的临床重要性。

本发明的核酸和蛋白质分子也可用作基因组特异性区域的标记。它不仅可用于基因组图谱，也可用于谷氨酸棒状杆菌蛋白质的功能研究。例如，可切割谷氨酸棒状杆菌基因组，并将片段与DNA-结合蛋白孵育来确定与特定的谷氨酸棒状杆菌DNA-结合蛋白结合的基因组区域。另外，那些结合蛋白的片段可用本发明的核酸分子探测，优选地用具有易于检测的标记物的核酸分子探测；此核酸分子与基因组片段的结合可将片段定位于谷氨酸棒状杆菌的基因组图谱中，并且当用不同的酶进行多次时，利于快速确定蛋白质结合的核酸序列。而且，本发明的核酸分子可充分同源于亲缘种的序列，这样这些核酸分子可用作在亲缘菌如乳糖发酵短杆菌中构建基因组图谱的标记。

本发明的MR核酸分子也用于进化研究和蛋白质结构研究。大量的原核和真核细胞应用本发明分子参与的代谢过程；通过将本发明核酸分子的序列与那些来自其它生物体的编码类似酶的序列比较，可评估所述生物体的进化关系。所以，此比较使得可评估哪些区域保守和哪些区域不保守，这有利于确定那些对酶功能所必需的蛋白质区域。这种确定对蛋白质工程研究有价值，并可指出该蛋白质可耐受何种诱变且不丢失其功能。

本发明MR核酸分子的操作可导致产生与野生型MR蛋白功能不同的MR蛋白。这些蛋白质可在功效或活性上提高，在细胞内可以较通常多的量存在，或可在功效或活性上降低。

这些活性的改变可以是使得谷氨酸棒状杆菌的一种或多种精细化学品的产量、生产率和/或生产效率提高的改变。通过优化活化编码目标精细化学品的生物合成蛋白的基因的转录或翻译或者通过影响或删除抑制这种基因转录或翻译的MR蛋白的活性，可能增加该生物合成途径的活性或活性率，归功于例如一种限制酶的量增加。相应地，可以通过改变MR蛋白的活性，使得其组成性地转录后失活参与目标精细化学品的降解途径的蛋白或者通过改变MR蛋白的活性，使得其组成性地抑制这种基因的转录或翻译以增加细胞的所述精细化学品的产量和/或生产率，这归功于该化合物的降解减少。

调节一种或多种MR蛋白的活性使得可能间接刺激细胞的一种或多种精细化学品的生产或者提高生产率，因为各种代谢途径相关联。可例如通过活化赖氨酸生物合成中一种或多种酶的表达来增加产量、生产率和/或生产效率以同时增加其他化合物的表达，例如其他氨基酸，当需要更大量的赖氨酸时细胞通常需要更大量的这些化合物。还可以改变整个细胞中的代谢调节，使得发酵培养物(其中营养和氧气的供给可能较差并且该环境中还可能大量存在毒性废物)的环境条件中的细胞的生长和复制改善。从而可例如改善培养物中细胞的生长和增殖，即使生长条件不是最佳，通过诱变某种MR蛋白(其针对胞外培养基中的高水平废产物抑制细胞膜生长所需的分子的合成以便阻碍非最佳生长条件中的细胞生长和细胞分裂)使得所述蛋白不再能够抑制所述合成。这种生长增加或这种存活增加将同样增加发酵培养物中目标精细化学品的产量和/或生产率，因为在给培养物中相对更多数目的细胞生产该化合物。

上述用于增加谷氨酸棒状杆菌目标化合物产量的MR蛋白的诱变策略不用于限制；对这些诱变策略的变化对技术人员是显而易见的。本文公开的这些策略和机制使得可以使用本发明的核酸和蛋白质分子以产生表达突变的MR核酸和蛋白质分子的谷氨酸棒状杆菌或相关的细菌菌株，从而提高目标化合物的产量、生产率和/或产生效率。该目标化合物可为谷氨酸棒状杆菌的任一天然产物，其包括生物合成途径的终产物和天然代谢途径的中间体，该目标化合物也可为在谷氨酸棒状杆菌代谢中非天然存在的分子，但该分子为本发明的谷氨酸棒状杆菌菌株产生。

不应将下面的实施例理解为对限制对本发明的进一步阐述。在该申请中引用的所有参考文献、专利申请、专利、公开的专利申请、表的全部内容此处引用作为参考。

实施例

实施例1：谷氨酸棒状杆菌ATCC 13032的总基因组DNA的制备

谷氨酸棒状杆菌(ATCC 13032)的培养物在30℃于BHI培养基(Difco)中剧烈振摇过夜。离心收获细胞，弃上清并将细胞重悬于5ml缓冲液-I(培养物原初体积的5％—所有陈述的体积按100ml培养物体积计算)。缓冲液-I的组成：140.34g/l蔗糖，2.46g/l MgSO₄·7H₂O，10ml/l KH₂PO₄溶液(100g/l，用KOH调节至pH6.7)，50ml/l M12浓缩物(10g/l(NH₄)₂SO₄，1g/lNaCl，2g/l MgSO₄·7H₂O，0.2g/l CaCl₂，0.5g/l酵母提取物(Difco)，10ml/l痕量元素混合物(200mg/l FeSO₄·H₂O，10mg/l ZnSO₄·7H₂O，3mg/lMnCl₂·4H₂O，30mg/l H₃BO₃，20mmg/l CoCl₂·6H₂O，1mg/l NiCl₂·6H₂O，3mg/l Na₂MoO₄·2H₂O，500mg/l络合剂(EDTA或柠檬酸)，100ml/l维生素-混合物(0.2mg/l生物素，0.2mg/l叶酸，20mg/l对氨基苯甲酸，20mg/l核黄素，40mg/l Ca-泛酸盐，140mg/l烟酸，40mg/l维生素B6盐酸盐，200mg/l肌醇)。向悬液中加入溶菌酶至终浓度2.5mg/ml。在37℃孵育约4小时后，细胞壁降解，离心收获所得的原生质体。沉淀用5ml缓冲液-I洗一次并用5ml TE-缓冲液(10mM Tris-HCl，1mM EDTA，pH8)洗一次。将沉淀重悬于4ml TE-缓冲液，并加入0.5ml SDS溶液(10％)和0.5ml NaCl溶液(5M)。加入蛋白酶K至终浓度200μg/ml后，将悬液在37℃孵育约18小时。使用标准方法用酚、酚-氯仿-异戊醇和氯仿-异戊醇提取而纯化DNA。然后，加入1/50体积的3M醋酸钠和2体积的乙醇，于-20℃孵育30分钟，并在高速离心机上使用SS34转子(Sorvall)于12,000转/分离心30分钟，沉淀DNA。将该DNA溶于含20μg/ml RNaseA的1ml TE-缓冲液中，并用1000ml TE-缓冲液于4℃透析至少3小时。在此期间，更换缓冲液3次。向分装的0.4ml透析过的DNA溶液中加入0.4ml 2M LiCl和0.8ml乙醇。于-20℃孵育30分钟后，离心(13,000转/分，Biofuge Fresco，Heraeus，Hanau，德国)收集DNA。将该DNA沉淀溶于TE-缓冲液。用此方法制备的DNA可用于所有目的，包括Southern印迹或构建基因组文库。

实施例2：在大肠杆菌中构建谷氨酸棒状杆菌(ATCC13032)的基因组文库

从如实施例1所述制备的DNA开始，按照公知的且已建立的方法(参见例如，Sambrook，J.等人(1989)“分子克隆：实验室手册”(“MolecularCloning：A Laboratory Manual”)，Cold Spring Harbor Laboratory Press，或Ausubel，F.M.等人(1994)“分子生物学最新方法”(“Current Protocolsin Molecular Biology”)，John Wiley&Sons.)构建粘粒和质粒文库。

可使用任一质粒或粘粒。特别使用的是质粒pBR322(Sutcliffe，J.G.(1979)美国国家科学院院报(Proc.Natl.Acad.Sci.USA)，75：3737-3741)；pACYC177(Change&Cohen(1978)细菌学杂志(J.Bacteriol)134：1141-1156)、pBS系列的质粒(pBSSK+，pBSSK-及其它；Stratagene，LaJolla，USA)、或粘粒如SuperCos1(Stratagene，LaJolla，USA)或Lorist6(Gibson，T.J.，Rosenthal A.和Waterson，R.H.(1987)基因(Gene)53：283-286)。

实施例3：DNA序列测定和功能的计算分析

如实施例2所述的基因组文库按照标准方法用于DNA序列测定，尤其是使用ABI377序列测定仪通过链终止法测定(参见例如，Fleischman，R.D.等人(1995)，流感嗜血菌(Haemophilus Influenzae)Rd的全基因组随机序列测定和组装，科学(Science)，269：496-512)。使用具有以下核苷酸序列的测序引物：5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。

实施例4：体内诱变

谷氨酸棒状杆菌的体内诱变可通过将质粒(或其它载体)DNA在不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如Bacillus spp.或酵母如酿酒酵母)中传代而实施。常见的突变基因菌株在用于DNA修复系统的基因中有突变(例如mutHLS，mutD，mutT等；参考文献见大肠杆菌和沙门菌(Escherichia coli and Salmonella)，ASM：Washington一书中的Rupp，W.D.(1996)DNA修复机制，2277-2294页)。此类菌株为技术人员熟知。此类菌株的用途在例如Greener，A.和Callahan，M.(1994)Strategies 7：32-34中进行了阐述。

实施例5：在大肠杆菌和谷氨酸棒状杆菌之间的DNA转移

几种棒状杆菌和短杆菌种类含有自主复制的内源质粒(例如pHM1519或pBL1)(综述参见例如，Martin，J.F.等人(1987)生物技术(Biotechnology)，5：137-146)。使用标准的大肠杆菌载体易于构建大肠杆菌和谷氨酸棒状杆菌的穿梭载体(Sambrook，J.等人(1989)，“分子克隆：实验室手册”(“Molecular Cloning：A Laboratory Manual”)，Cold Spring HarborLaboratory Press，或Ausubel，F.M.等人(1994)“最新分子生物学方法”(“Current Protocols in Molecular Biology”)，John Wiley&Sons)，其中加入复制原点和来自谷氨酸棒状杆菌的适当标记。此类复制原点优选地取自从棒状杆菌和短杆菌分离的内源质粒。这些种类中作为转化标记尤其有用的是卡那霉素抗性基因(如衍生自Tn5或Tn903转座子的基因)或氯霉素抗性基因(Winnacker，E.L.(1987)，From Genes to Clones-Introductionto Gene Technology，VCH，Weinheim)。在文献中有许多在大肠杆菌和谷氨酸棒状杆菌中均复制的多种穿梭载体的例子，且其可用于多个目的，包括基因过表达(参考文献参见例如，Yoshihama，M.等人(1985)细菌学杂志(J.Bacteriol.)162：591-597，Martin J.F.等人(1987)生物技术(Biotechnology)，5：137-146和Eikmanns，B.J.等人(1992)基因(Gene)，102：93-98)。

使用标准技术，可将目的基因克隆入上述的穿梭载体之一并可将此杂合载体导入谷氨酸棒状杆菌菌株。谷氨酸棒状杆菌的转化可通过原生质体转化(Kastsumata，R.等人(1984)细菌学杂志(J.Bacteriol.)159，306-311)、电穿孔(Liebl，E.等人(1989)FEMS Microbiol.Letters，53：399-303)以及当使用特别的载体时，也通过接合(如在Sch_fer，A等人(1990)细菌学杂志(J.Bacteriol.)172：1663-1666中所述)来完成。也可转移谷氨酸棒状杆菌的穿梭载体至大肠杆菌，其为通过制备来自谷氨酸棒状杆菌的质粒DNA(使用本领域公知的标准方法)并将其转化至大肠杆菌。该转化步骤可使用标准方法进行，但有利的是使用Mcr-缺陷的大肠杆菌菌株，如NM522(Gough&Murray(1983)分子生物学杂志(J.Mol.Biol.)166：1-19)。

实施例6：突变蛋白质的表达的确定

在转化的宿主细胞中对突变蛋白质活性的观察依赖于突变蛋白质以与野生型蛋白质相似的方式和相似的量表达的事实。确定突变基因转录水平(指示可用于翻译为基因产物的mRNA量)的有用方法是进行Northern印迹(参考文献见例如，Ausubel等人(1988)分子生物学最新方法(CurrentProtocols in Molecular Biology)，Wiley：New York)，其中设计为与目的基因结合的引物用可检测标志(通常为放射性的或化学发光的)标记，这样当提取生物体培养物的总RNA、在凝胶上运行、转移至稳定的基质并与该探针孵育时，探针的结合和结合量指示了该基因的存在和其mRNA量。该信息是突变基因转录程度的证据。通过几种方法可从谷氨酸棒状杆菌中分离总细胞RNA，这些方法均为本领域熟知，如在Bormann，E.R.等人(1992)分子微生物学(Mol.Microbiol.)6：317-326中所描述。

为了确定从该mRNA翻译的蛋白质的存在或相对量，可使用标准技术如Western印迹(参见例如，Ausubel等人(1988)分子生物学最新方法(Current Protocols in Molecular Biology)，Wiley：New York)。在该方法中，提取细胞总蛋白质、通过凝胶电泳分离、转移至基质如硝化纤维并与探针孵育，其中所述探针如与目标蛋白质特异性结合的抗体。该探针通常用易于检测到的化学发光或显色标记物标记。所观察到的标记物的存在和量指示了在细胞中目标突变蛋白质的存在和量。

实施例7：遗传改变的谷氨酸棒状杆菌的生长—培养基和培养条件

遗传修饰的棒状杆菌培养在合成或天然的生长培养基中。用于棒状杆菌的许多种不同生长培养基为人们熟知并易于获得(Lieb等人(1989)Appl.Microbiol.Biotechnol.，32：205-210；Von der Osten等人(1998)Biotechnology Letters，11：11-16；Patent DE 4,120,867；在原核生物(TheProcaryotes)，Volume II，Balows，A.等人编辑.Springer-Verlag一书中的Liebl(1992)“棒状杆菌属”(“The Genus Corynebacteria”))。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源是糖类，如单糖、二糖或或多糖。优良碳源的实例是葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可通过复合化合物如糖蜜或其它来自糖加工的副产品提供糖至培养基。提供不同碳源的混合物也可是有利的。其它可能的碳源为醇类和有机酸，如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物，或为包含这些化合物的物质。示例性氮源包括氨气或胺盐如NH₄Cl或(NH₄)₂SO₄、NH₄OH、硝酸盐、尿素、氨基酸和复合氮源如玉米浆、大豆粉、大豆蛋白质、酵母提取物、肉羹等。

可包括在培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、亚磷酸盐或硫酸盐。可向培养基中加入螯合物以维持溶液中的金属离子。尤其有用的螯合物包括二羟基酚，如儿茶酚或原儿荼酚盐，和有机酸如柠檬酸。培养基一般也包括其它生长因子如维生素或生长促进剂，其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和维生素B₆。生长因子和盐通常来自复合的培养基组成，如酵母提取物、糖蜜、玉米浆等。培养基化合物的精确组成强烈取决于直接实验并在每一具体情况下单独确定。有关培养基优化的信息在课本“Applied Microbiol.Physiology，A Practical Approach”(P.M.Rhodes，P.F.Stanbury编辑，IRL Press(1997)53-73页，ISBN 0 19 9635773)中可找到。也可从供应商处得到生长培养基，如Standard 1(Merck)或BHI(brain heart infusion，DIFCO)和其它。

通过加热(1.5巴，121℃加热20分钟)或过滤除菌对所有的培养基组成成份进行灭菌。组成成份可一起灭菌，或需要的话分开灭菌。所有的培养基组成成份可在培养开始时存在，或它们可任选地连续加入或分批加入。

对每一实验分别定义培养条件。温度应在15℃和45℃的范围之间。在实验中温度可保持恒定或可改变。培养基的pH应在5至8.5的范围之间，优选地在7.0附近，可通过向培养基中添加缓冲液而维持。用于此目的的示例性缓冲液为磷酸钾缓冲液。可备选地或同时使用合成缓冲液如MOPS、HEPES、ACES和其它。在生长过程中通过加入NaOH或NH₄OH也可维持恒定的培养pH。如果使用复合的培养基组成如酵母提取物，可减轻对额外的缓冲液的需要，因为许多复合化合物具有高缓冲能力。如果使用发酵罐培养微生物，用气态氨也可控制pH。

培养时间通常在几小时到几天之间。选择培养时间以允许肉汤中累积最大量的产物。可在多种容器如不同规格的微滴定板、玻璃管、玻璃烧瓶或玻璃或金属发酵罐中实施公开的生长实验。对于筛选大量的克隆，应在微滴定板、玻璃管或在有或没有挡板的摇瓶中培养微生物。优选地使用100ml摇瓶，其中装有10％(体积)的所需生长培养基。该烧瓶应在旋转振荡器(振幅25mm)上以100-300转/分摇动。可通过维持潮湿环境减少蒸发损失；备选地，对蒸发损失应进行数学上的校正。

如果试验遗传修饰的克隆，也应试验未修饰的对照克隆或含基本质粒的无任何插入的对照克。使用已于30℃孵育的在琼脂板上生长的细胞接种培养基至OD₆₀₀ 0.5-1.5，其中所述琼脂板如CM板(10g/l葡萄糖，2.5g/lNaCl，2g/l尿素，10g/l聚胨，5g/l酵母抽取物，5g/l肉羹，22g/l琼脂，用2M NaOH调节至pH6.8)。培养基的接种通过导入来自CM板的谷氨酸棒状杆菌细胞的盐水悬液或添加该细菌的原液体培养物而完成。

实施例8：突变蛋白质功能的体外分析

本领域已建立了对酶活性和动力学的确定。确定特定的改变酶的活性的实验应加以修改，使适应野生型酶特异活性的确定，这在本领域普通技术人员的能力范围内。可在下列参考文献中找到对酶从总体上的概观，以及有关结构、动力学、原理、方法、应用和对许多酶活性进行确定的例子的具体说明，例如Dixon，M.和Webb，E.C.，(1979)酶(Enzymes).Longmans：伦敦；Fersht，(1985)酶结构和机制(Enzyme Structure andMechanism).Freeman：纽约；Walsh，(1979)酶促反应机制(EnzymaticReaction Mechanisms).Freeman：San Francisco；Price，N.C.，Stevens，L.(1982)酶学基础(Fundamentals of Enzymology).Oxford Univ.Press：Oxford；Boyer，P.D.编辑(1983)酶(The Enzymes)，第三版，Academic Press：纽约；Bisswanger，H.，(1994)酶动力学(Enzymkinetik)，第二版.VCH：Weinheim(ISBN 3527300325)；Bergmeyer，H.U.，Bergmeyer，J.，Graβl，M.编辑(1983-1986)酶促分析方法(Methods of Enzymatic Analysis)，第三版，I-XII卷，Verlag Chemie：Weinheim；以及Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)(1987)A9卷，“酶”.VCH：Weinheim，352-363页。

可通过几种已建立的方法测定结合DNA的蛋白质的活性，如DNA带移动试验(也称为凝胶阻滞试验)。此类蛋白质对其它分子表达的影响可使用报告基因试验测定(如在Kolmar，H.等人(1995)EMBO J.14：3895-3904及其中所引用的参考文献中所述)。报告基因测试系统为人们所熟知，并被建立以应用于原核和真核细胞，使用的酶如β-半乳糖苷酶、绿色荧光蛋白和几种其它的酶。

可根据如在生物膜，分子结构和功能(Biomembranes.MolecularStructure and Function)，Springer：Heidelberg一书中的Gennis，R.B.(1989)“Pores，Channels and Transporters”，85-137；199-234和270-322页中所述的技术进行膜转运蛋白活性的测定。

实施例9：分析突变蛋白质对目标产物产生的影响

谷氨酸棒状杆菌中的遗传修饰对目标化合物(如氨基酸)产生的影响可通过将经修饰的微生物在合适的条件(如上文中所描述的那些条件)下生长并分析用于增加目标产物(即氨基酸)产生的培养基和/或细胞组成来确定。此类分析技术为技术人员熟知，包括光谱学、薄层色谱法、多种染色法、酶促和微生物学方法、以及分析色谱法如高效液相色谱法(参见例如，Ullman工业化学百科全书(Ullman，Encyclopedia of IndustrialChemistry)，A2卷，89-90和443-613页，VCH：Weinheim(1985)；Fallon，A.等人(1987)生物化学和分子生物学实验室技术(Laboratory Techniques inBiochemistry and Molecular Biology)中的“HPLC在生物化学中的应用”(“Applications of HPLC in Biochemistry”)，17卷；Rehm等人(1993)生物技术(Biotechnology)，3卷，III章“产物回收和纯化”(“Productrecovery and purification”)，469-714页，VCH：Weinheim；Belter，P.A.等人(1988)生物分离：生物技术中的下游加工(Bioseparations：downstreamprocessing for biotechnology)，John Wiley and Sons；Kennedy，J.F.和Cabral，J.M.S.(1992)生物物质的回收方法(Recovery processes forbiological materials)John Wiley and Sons；在Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)一书中的Shaeiwitz，J.A.和Henry，J.D.(1988)生物化学分离(Biochemical separations)，B3卷，11章，1-27页，VCH：Weinheim；以及Dechow，F.J.(1989)生物技术中的分离和纯化技术(Separation and purification techniques inbiotech nology)，Noyes Publications.)。

除了测定发酵终产物外，还可分析用于产生目标化合物的代谢途径的其它组成如中间体和副产物来确定化合物的总体产生力、化合物的产率和/或生产效率。分析方法包括测定培养基中的营养素水平(例如，糖类、碳水化合物、氮源、磷酸盐和其它离子)、测定生物量组成和生长、分析生物合成途径中共同代谢物的产生及测定发酵过程中产生的气体。这些测定的标准方法在应用微生物生理学，实用方法(Applied Microbial Physiology，APractical Approach)，P.M.Rhodes和P.F.Stanbury编辑，IRL Press，103-129；131-163和165-192页(ISBN：0199635773)及其引用的参考文献中列出。

实施例10：从谷氨酸棒状杆菌培养物中纯化目标产物

可通过多种本领域熟知的方法实施从谷氨酸棒状杆菌细胞或上述培养物上清回收目标产物。如果目标产物不从细胞中分泌，可低速离心从培养物中收获细胞、用标准技术如机械力或超声裂解细胞。离心去除细胞碎片，保留含有可溶蛋白质的上清级分用于进一步纯化目标化合物。如果化合物从谷氨酸棒状杆菌细胞分泌，则低速离心从培养物中去除细胞，并保留上清级分用于进一步纯化。

将来自两种中任一纯化方法的上清级分用合适的树脂进行层析，其中目标分子保留在层析树脂上而样本中的许多杂质不保留在层析树脂上，或者杂质保留在层析树脂上而样本不保留。此层析步骤必需时可重复，其中使用相同或不同的层析树脂。技术人员在选择合适的层析树脂以及将它们最有效地用于特定的待纯化分子方面是很精通的。可通过过滤或超滤浓缩纯化产物，并贮存在使产物稳定性最高的温度下。

有大量的纯化方法为本领域公知，上述纯化方法不用于限制。此类纯化技术例如在Bailey，J.E.和Ollis，D.F.生物化学工程基础(BiochemicalEngineering Fundamentals)，McGraw-Hill：纽约(1986)中描述。

分离化合物的特性和纯度可通过本领域的标准技术确定。这些技术包括高效液相色谱(HPLC)、光谱法、染色法、薄层色谱法、NIRS、酶促试验或微生物学方法。此类分析方法在Patek等人(1994)Appl.Environ.Microbiol.60：133-140；Malakhova等人(1996)生物技术(Biotekhnologiya)11：27-32；和Schmidt等人(1998)生物加工工程(Bioprocess Engineer)19：67-70.Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)(1996)A27卷，VCH：Weinheim，89-90页，521-540页，540-547页，559-566页，575-581页和581-587页；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，John Wiley and Sons；生物化学和分子生物学中的实验室技术(Laboratory Techniques in Biochemistry and Molecular Biology)，17卷中的Fallon，A.等人(1987)HPLC在生物化学中的应用(Applications ofHPLC in Biochemistry)。

等同物

技术人员知道或通过简单的常规方法可确定许多于文中所描述本发明具体实施方案的等同物。此类等同物旨在包括在下面的专利权利要求书中。

表1中的信息理解如下：

在第1列中，“DNA ID”，每种情况下相关数字指所公开序列表的SEQ ID NO。所以，“DNA ID”列中的“5”指SEQ ID NO：5。

在第2列中，“AA ID”，每种情况下相关数字指所公开序列的SEQ IDNO。“AA ID”列中的“6”指SEQ ID NO：6。

在第3列中，列出了“标识”——每个序列的明确内部名称。

在第4列中，“AA pos”，每种情况下相关数字指相同行中多肽序列“AA ID”的氨基酸位置。所以，“AA pos”列中“26”是相应所示的多肽序列的氨基酸位置26。位置计算从N末端的+1开始。

在第5列中，“AA野生型”，每种情况下相关数字指相应野生型菌株在第4列中所示位置的氨基酸(用单字母代码)。

在第6列中，“AA突变株”，每种情况下相关数字指相应突变菌株在第4列中所示位置的氨基酸(用单字母代码)。

在第7列中，列出了“功能”——相应多肽序列的生理学功能。

蛋白原氨基酸的单字母代码：

A 丙氨酸

C 半胱氨酸

D 天冬氨酸

E 谷氨酸

F 苯丙氨酸

G 甘氨酸

H 组氨酸

I 异亮氨酸

K 赖氨酸

L 亮氨酸

M 甲硫氨酸

N 天冬酰胺

P 脯氨酸

Q 谷氨酰胺

R 精氨酸

S 丝氨酸

T 苏氨酸

V 缬氨酸

W 色氨酸

Y 酪氨酸

表1

编码调节蛋白的基因

DNA ID： AA ID：标识 AA位置： AA野生型 AA突变体功能：

1 2 RXA00205 220 G D 核糖操纵子阻抑蛋白

3 4 RXA00253 177 T I 四环素阻抑蛋白E类

5 6 RXA00291 219 E K 传感蛋白激酶DPIB(EC 2.7.3.-)

7 8 RXA00319 63 A V 低分子量磷酸酪氨酸蛋白磷酸酶(EC 3.1.3.48)

9 10 RXA00486 80 R H 转录调节蛋白，LYSR家族

11 12 RXA00593 18 A V 细胞分裂转录因子，WHMD

13 14 RXA00609 107 A T 转录调节蛋白

15 16 RXA00651 374 G D 感觉转导蛋白激酶(EC 2.7.3.-)

17 18 RXA00719 306 D N GTP-结合蛋白

468 A T GTP-结合蛋白

19 20 RXA00813 340 G D 分泌性蛋白激酶

21 22 RXA01081 102 R C 转录调节蛋白

23 24 RXA01315 20 A T 转录调节子，TETR家族

101 G D 转录调节子，TETR家族

25 26 RXA01573 474 P S 2’，3’-环核苷酸2’-磷酸二酯酶(EC 3.1.4.16)

27 28 RXA01607 142 R W 转录调节蛋白

179 A T 转录调节蛋白

29 30 RXA01759 63 S F 转录调节蛋白GLTC

191 P S 转录调节蛋白GLTC

31 32 RXA01826 603 P S 丝氨酸/苏氨酸蛋白激酶(EC 2.7.1.37)

33 34 RXA02097 28 A V 转录调节子

427 G R 转录调节子

35 36 RXA02210 4 A V 转录调节子，TETR家族

37 38 RXA02362 525 M I 转录调节子

39 40 RXA02376 150 H R GTP-结合蛋白

41 42 RXA02627 338 E K DTXR/铁调节的脂蛋白前体

43 44 RXA02667 193 G D 转录调节子

45 46 RXA02758 110 G E 磷丝氨酸磷酸酶(EC 3.1.3.3)

47 48 RXA02910 186 D N 转录调节蛋白，LYSR家族

49 50 RXA03100 170 R C 酰胺脲结合蛋白前体

176 S F 酰胺脲结合蛋白前体

51 52 RXA03127 142 E K 转录调节蛋白

53 54 RXA03136 205 P S 转录活化子，LUXR家族

385 D N 转录活化子，LUXR家族

55 56 RXA03201 206 D N UDP-葡萄糖4-差向异构酶(EC 5.1.3.2)

57 58 RXA03407 70 A V 转录调节子

59 60 RXA03629 36 S N 转录调节子

61 62 RXA03928 170 E R 转录调节子

171 A S 转录调节子

172 M H 转录调节子

63 64 RXA04129 54 A V 转录调节子，ARSR家族

65 66 RXA04350 150 R H 转录调节蛋白

67 68 RXA04363 81 S F 转录阻抑蛋白

69 70 RXA04620 260 R H 转录调节蛋白

71 72 RXA06017 33 A V 可能的过氧化氢诱导的基因活化子

73 74 RXA07002 158 P S 传感蛋白BAES(EC 2.7.3.-)

75 76 RXA07003 126 L F 转录调节蛋白

195 R K 转录调节蛋白

序列表

<110>巴斯福股份公司

<120>来自谷氨酸棒状杆菌的编码调节蛋白的基因

<130>O.Z.0050/52970

<160>76

<210>1

<211>1294

<212>DNA

<213>谷氨酸棒状杆菌(Corynebacterium glutamicum)

<220>

<221>CDS

<222>(101)..(1264)

<223>RXA00205

<400>1

gaaatcaagt ggcctagatc cattgacact tagactgtga cctaggcttg actttcgtgg 60

gggagtgggg ataagttcat cttaaacaca atgcaatcga ttg cat tta cgt tcc 115

Leu His Leu Arg Ser

1 5

tta tcc cac aat agg ggt acc ttc cag aaa gtt ggt gag gag atg gct 163

Leu Ser His Asn Arg Gly Thr Phe Gln Lys Val Gly Glu Glu Met Ala

10 15 20

tcc gaa acc tcc agc ccg aag aag cgg gcc acc acg ctc aaa gac atc 211

Ser Glu Thr Ser Ser Pro Lys Lys Arg Ala Thr Thr Leu Lys Asp Ile

25 30 35

gcg caa gca aca cag ctt tca gtc agc acg gtg tcc cgg gca ttg gcc 259

Ala Gln Ala Thr Gln Leu Ser Val Ser Thr Val Ser Arg Ala Leu Ala

40 45 50

aac aac gcg agc att ccg gaa tcc aca cgc atc cga gtg gtt gaa gcc 307

Asn Asn Ala Ser Ile Pro Glu Ser Thr Arg Ile Arg Val Val Glu Ala

55 60 65

gct caa aag ctg aac tac cgt ccc aat gcc caa gct cgt gca ttg cgg 355

Ala Gln Lys Leu Asn Tyr Arg Pro Asn Ala Gln Ala Arg Ala Leu Arg

70 75 80 85

aag tcg agg aca gac acc atc ggt gtc atc att cca aac att gag aac 403

Lys Ser Arg Thr Asp Thr Ile Gly Val Ile Ile Pro Asn Ile Glu Asn

90 95 100

cca tat ttc tcc tca cta gca gca tcg att caa aaa gct gct cgt gaa 451

Pro Tyr Phe Ser Ser Leu Ala Ala Ser Ile Gln Lys Ala Ala Arg Glu

105 110 115

gct ggg gtg tcc acc att ttg tcc aac tct gaa gaa aac cca gag ctg 499

Ala Gly Val Ser Thr Ile Leu Ser Asn Ser Glu Glu Asn Pro Glu Leu

120 125 130

ctt ggt cag act ttg gcg atc atg gat gac caa cgc ctc gat gga atc 547

Leu Gly Gln Thr Leu Ala Ile Met Asp Asp Gln Arg Leu Asp Gly Ile

135 140 145

atc gtg gtg cca cac att cag tca gag gaa caa gtc act gac ttg gtt 595

Ile Val Val Pro His Ile Gln Ser Glu Glu Gln Val Thr Asp Leu Val

150 155 160 165

aac agg gga gtg cca gta gtg ctg gca gac cgt agt ttt gtt aac tcg 643

Asn Arg Gly Val Pro Val Val Leu Ala Asp Arg Ser Phe Val Asn Ser

170 175 180

tct att cct tcg gtt acc tca gat cca gtt ccg ggc atg act gaa gct 691

Ser Ile Pro Ser Val Thr Ser Asp Pro Val Pro Gly Met Thr Glu Ala

185 190 195

gtg gac tta ctc ctg gca gct gac gtg caa ttg ggc tac ctt gcc ggc 739

Val Asp Leu Leu Leu Ala Ala Asp Val Gln Leu Gly Tyr Leu Ala Gly

200 205 210

ccg cag gat act tcc act ggt cag ctg cgt ctt aac act ttt gaa aga 787

Pro Gln Asp Thr Ser Thr Gly Gln Leu Arg Leu Asn Thr Phe Glu Arg

215 220 225

cta tgc gtg gac cgc ggc atc gtc gga gca tct gtc tat tac ggt ggc 835

Leu Cys Val Asp Arg Gly Ile Val Gly Ala Ser Val Tyr Tyr Gly Gly

230 235 240 245

tac cgc caa gaa tct gga tat gac ggc atc aag gtg ctg atc aag cag 883

Tyr Arg Gln Glu Ser Gly Tyr Asp Gly Ile Lys Val Leu Ile Lys Gln

250 255 260

gga gcc aat gcg att atc gct ggt gac tcc atg atg acc atc ggt gcg 931

Gly Ala Asn Ala Ile Ile Ala Gly Asp Ser Met Met Thr Ile Gly Ala

265 270 275

ttg ttg gct ctt cat gag atg aat ttg aag atc ggt gag gat gtg cag 979

Leu Leu Ala Leu His Glu Met Asn Leu Lys Ile Gly Glu Asp Val Gln

280 285 290

ctc att ggg ttt gat aac aac cca att ttc cgg ctg cag aat cca ccg 1027

Leu Ile Gly Phe Asp Asn Asn Pro Ile Phe Arg Leu Gln Asn Pro Pro

295 300 305

ctg agc atc att gac cag cac gta caa gag atc ggt aag cgt gcg ttt 1075

Leu Ser Ile Ile Asp Gln His Val Gln Glu Ile Gly Lys Arg Ala Phe

310 315 320 325

gag att ctg cag aag ctg atc aat ggg gac acc gcg caa aaa tct gtg 1123

Glu Ile Leu Gln Lys Leu Ile Asn Gly Asp Thr Ala Gln Lys Ser Val

330 335 340

gtg att cca acg cag ctc agc atc aat gga tca acg gcg gtt tcc caa 1171

Val Ile Pro Thr Gln Leu Ser Ile Asn Gly Ser Thr Ala Val Ser Gln

345 350 355

aag gcg gcc gca aag gca gca aaa gca gcc caa aaa gca gcc gcg aaa 1219

Lys Ala Ala Ala Lys Ala Ala Lys Ala Ala Gln Lys Ala Ala Ala Lys

360 365 370

gcc gca cag aac acg caa cac gag gtg agc cta gat ggt gaa ctc 1264

Ala Ala Gln Asn Thr Gln His Glu Val Ser Leu Asp Gly Glu Leu

375 380 385

tgaacaagcg cttcatcagc atgatcctgc 1294

<210>2

<211>388

<212>PRT

<213>谷氨酸棒状杆菌

<400>2

Leu His Leu Arg Ser Leu Ser His Asn Arg Gly Thr Phe Gln Lys Val

1 5 10 15

Gly Glu Glu Met Ala Ser Glu Thr Ser Ser Pro Lys Lys Arg Ala Thr

20 25 30

Thr Leu Lys Asp Ile Ala Gln Ala Thr Gln Leu Ser Val Ser Thr Val

35 40 45

Ser Arg Ala Leu Ala Asn Asn Ala Ser Ile Pro Glu Ser Thr Arg Ile

50 55 60

Arg Val Val Glu Ala Ala Gln Lys Leu Asn Tyr Arg Pro Asn Ala Gln

65 70 75 80

Ala Arg Ala Leu Arg Lys Ser Arg Thr Asp Thr Ile Gly Val Ile Ile

85 90 95

Pro Asn Ile Glu Asn Pro Tyr Phe Ser Ser Leu Ala Ala Ser Ile Gln

100 105 110

Lys Ala Ala Arg Glu Ala Gly Val Ser Thr Ile Leu Ser Asn Ser Glu

115 120 125

Glu Asn Pro Glu Leu Leu Gly Gln Thr Leu Ala Ile Met Asp Asp Gln

130 135 140

Arg Leu Asp Gly Ile Ile Val Val Pro His Ile Gln Ser Glu Glu Gln

145 150 155 160

Val Thr Asp Leu Val Asn Arg Gly Val Pro Val Val Leu Ala Asp Arg

165 170 175

Ser Phe Val Asn Ser Ser Ile Pro Ser Val Thr Ser Asp Pro Val Pro

180 185 190

Gly Met Thr Glu Ala Val Asp Leu Leu Leu Ala Ala Asp Val Gln Leu

195 200 205

Gly Tyr Leu Ala Gly Pro Gln Asp Thr Ser Thr Gly Gln Leu Arg Leu

210 215 220

Asn Thr Phe Glu Arg Leu Cys Val Asp Arg Gly Ile Val Gly Ala Ser

225 230 235 240

Val Tyr Tyr Gly Gly Tyr Arg Gln Glu Ser Gly Tyr Asp Gly Ile Lys

245 250 255

Val Leu Ile Lys Gln Gly Ala Asn Ala Ile Ile Ala Gly Asp Ser Met

260 265 270

Met Thr Ile Gly Ala Leu Leu Ala Leu His Glu Met Asn Leu Lys Ile

275 280 285

Gly Glu Asp Val Gln Leu Ile Gly Phe Asp Asn Asn Pro Ile Phe Arg

290 295 300

Leu Gln Asn Pro Pro Leu Ser Ile Ile Asp Gln His Val Gln Glu Ile

305 310 315 320

Gly Lys Arg Ala Phe Glu Ile Leu Gln Lys Leu Ile Asn Gly Asp Thr

325 330 335

Ala Gln Lys Ser Val Val Ile Pro Thr Gln Leu Ser Ile Asn Gly Ser

340 345 350

Thr Ala Val Ser Gln Lys Ala Ala Ala Lys Ala Ala Lys Ala Ala Gln

355 360 365

Lys Ala Ala Ala Lys Ala Ala Gln Asn Thr Gln His Glu Val Ser Leu

370 375 380

Asp Gly Glu Leu

385

<210>3

<211>868

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(838)

<223>RXA00253

<400>3

acccatgtta gatgttttat tcagggattt tagttgatat gtccagtatc tcgctgaaaa 60

cgctggttgt cttgtagaaa aaggcgtaac gtcatataac atg cct agc gaa act 115

Met Pro Ser Glu Thr

1 5

atg aaa cca gcc gta gcg tca act ctg gcg gcc act tcc acg gga cgt 163

Met Lys Pro Ala Val Ala Ser Thr Leu Ala Ala Thr Ser Thr Gly Arg

10 15 20

cgt cct gga cgc ccc acc caa cgt atc ctt tcc gtc gaa tcc ata gtg 211

Arg Pro Gly Arg Pro Thr Gln Arg Ile Leu Ser Val Glu Ser Ile Val

25 30 35

gag cgc act tta aac att gcc ggc cgc gaa gga ttc gct gcc gtg acc 259

Glu Arg Thr Leu Asn Ile Ala Gly Arg Glu Gly Phe Ala Ala Val Thr

40 45 50

atg aac cgc ctc gcc cga gac atg ggt gtc acc cct cgc gca ctg tat 307

Met Asn Arg Leu Ala Arg Asp Met Gly Val Thr Pro Arg Ala Leu Tyr

55 60 65

aac cat gtg cta aat cgt caa gaa atc att gat cgc gtc tgg gtg cgc 355

Asn His Val Leu Asn Arg Gln Glu Ile Ile Asp Arg Val Trp Val Arg

70 75 80 85

atc atc gat gat atc aag gtg ccc gat ctt gat ccg gac aat tgg cgg 403

Ile Ile Asp Asp Ile Lys Val Pro Asp Leu Asp Pro Asp Asn Trp Arg

90 95 100

caa tct att cat acg ctg tgg agc tca ttg cgc gac caa ttc cgt gag 451

Gln Ser Ile His Thr Leu Trp Ser Ser Leu Arg Asp Gln Phe Arg Glu

105 110 115

act cca cgt gtt ctt ctg gtc gcg ctg gat gaa cag atc tct act cag 499

Thr Pro Arg Val Leu Leu Val Ala Leu Asp Glu Gln Ile Ser Thr Gln

120 125 130

ggc act tcc cca ctg cga atc gcg ggt gcg gag gag tcc ttg aag ttc 547

Gly Thr Ser Pro Leu Arg Ile Ala Gly Ala Glu Glu Ser Leu Lys Phe

135 140 145

ttg act gat atc ggg ctg tcc ctc aag gaa gca acc atc atc cgg gag 595

Leu Thr Asp Ile Gly Leu Ser Leu Lys Glu Ala Thr Ile Ile Arg Glu

150 155 160 165

atg atg atg gct gat gtc ttc agc ttc acc ctg act tct gac tac acc 643

Met Met Met Ala Asp Val Phe Ser Phe Thr Leu Thr Ser Asp Tyr Thr

170 175 180

ttt gac aat cgt cca gag ggc gaa aag ccg gat gtg ttt gct ccg gtt 691

Phe Asp Asn Arg Pro Glu Gly Glu Lys Pro Asp Val Phe Ala Pro Val

185 190 195

cct aag cca tgg ctt gat gag aac cca gat gtg gaa gcg cca ctg acc 739

Pro Lys Pro Trp Leu Asp Glu Asn Pro Asp Val Glu Ala Pro Leu Thr

200 205 210

cgt aaa gca gtc gaa gag tcc gtc tca act tct gac gaa ctc ttc ggc 787

Arg Lys Ala Val Glu Glu Ser Val Ser Thr Ser Asp Glu Leu Phe Gly

215 220 225

tac atg gtg gag gct cgc att gct tat att gaa aag ctg ctt gcc gcc 835

Tyr Met Val Glu Ala Arg Ile Ala Tyr Ile Glu Lys Leu Leu Ala Ala

230 235 240 245

aaa tagtttctaa aggttattga gggcctgctc 868

Lys

<210>4

<211>246

<212>PRT

<213>谷氨酸棒状杆菌

<400>4

Met Pro Ser Glu Thr Met Lys Pro Ala Val Ala Ser Thr Leu Ala Ala

1 5 10 15

Thr Ser Thr Gly Arg Arg Pro Gly Arg Pro Thr Gln Arg Ile Leu Ser

20 25 30

Val Glu Ser Ile Val Glu Arg Thr Leu Asn Ile Ala Gly Arg Glu Gly

35 40 45

Phe Ala Ala Val Thr Met Asn Arg Leu Ala Arg Asp Met Gly Val Thr

50 55 60

Pro Arg Ala Leu Tyr Asn His Val Leu Asn Arg Gln Glu Ile Ile Asp

65 70 75 80

Arg Val Trp Val Arg Ile Ile Asp Asp Ile Lys Val Pro Asp Leu Asp

85 90 95

Pro Asp Asn Trp Arg Gln Ser Ile His Thr Leu Trp Ser Ser Leu Arg

100 105 110

Asp Gln Phe Arg Glu Thr Pro Arg Val Leu Leu Val Ala Leu Asp Glu

115 120 125

Gln Ile Ser Thr Gln Gly Thr Ser Pro Leu Arg Ile Ala Gly Ala Glu

130 135 140

Glu Ser Leu Lys Phe Leu Thr Asp Ile Gly Leu Ser Leu Lys Glu Ala

145 150 155 160

Thr Ile Ile Arg Glu Met Met Met Ala Asp Val Phe Ser Phe Thr Leu

165 170 175

Thr Ser Asp Tyr Thr Phe Asp Asn Arg Pro Glu Gly Glu Lys Pro Asp

180 185 190

Val Phe Ala Pro Val Pro Lys Pro Trp Leu Asp Glu Asn Pro Asp Val

195 200 205

Glu Ala Pro Leu Thr Arg Lys Ala Val Glu Glu Ser Val Ser Thr Ser

210 215 220

Asp Glu Leu Phe Gly Tyr Met Val Glu Ala Arg Ile Ala Tyr Ile Glu

225 230 235 240

Lys Leu Leu Ala Ala Lys

245

<210>5

<211>1783

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1753)

<223>RXA00291

<400>5

tgaggtagtg cgcaaaataa gacttttgtg cattatgatc agaattgttg gcctgggact 60

tcgcttcacg ctctgctgat aatcgccccc gggggtagac atg tct gtt ggt gga 115

Met Ser Val Gly Gly

1 5

tcc gac tgg aaa aac ttc aag gag gtg gac atc att cgc ttt gct acc 163

Ser Asp Trp Lys Asn Phe Lys Glu Val Asp Ile Ile Arg Phe Ala Thr

10 15 20

cga ata ctg gtg att caa gtg gct acc gtc gcg ttg gtg gta gct att 211

Arg Ile Leu Val Ile Gln Val Ala Thr Val Ala Leu Val Val Ala Ile

25 30 35

tgc acc gga att ttc gca gtt ttg atg atg gat cag atg aaa act gag 259

Cys Thr Gly Ile Phe Ala Val Leu Met Met Asp Gln Met Lys Thr Glu

40 45 50

gcc gag cac aca gcg ctg tcc atc gga cgt tcg gtg gca tcc aac ccg 307

Ala Glu His Thr Ala Leu Ser Ile Gly Arg Ser Val Ala Ser Asn Pro

55 60 65

cag atc cgc gag gaa gta gcg ctt gat act caa aca gga gca aac cca 355

Gln Ile Arg Glu Glu Val Ala Leu Asp Thr Gln Thr Gly Ala Asn Pro

70 75 80 85

tcg gcc gaa gaa tta gcc gat gga gat atc caa gcg gtt gca cag gcg 403

Ser Ala Glu Glu Leu Ala Asp Gly Asp Ile Gln Ala Val Ala Gln Ala

90 95 100

gcc aat gaa cgc act gga gct ttg ttt gtc gtt atc act gac ggt tta 451

Ala Asn Glu Arg Thr Gly Ala Leu Phe Val Val Ile Thr Asp Gly Leu

105 110 115

ggt atc cgc ctg tcc cac cca gat gag gaa cgt ctg ggg gag cag gtg 499

Gly Ile Arg Leu Ser His Pro Asp Glu Glu Arg Leu Gly Glu Gln Val

120 125 130

agc act agc ttt gag gct gcc atg cgg ggt gaa gaa acc atg gcg tgg 547

Ser Thr Ser Phe Glu Ala Ala Met Arg Gly Glu Glu Thr Met Ala Trp

135 140 145

gag act ggg acc ctc ggt gcg tcc gcg cga gca aaa gtg cct atc ttt 595

Glu Thr Gly Thr Leu Gly Ala Ser Ala Arg Ala Lys Val Pro Ile Phe

150 155 160 165

gcg ccg gat tct agt gtt cca gtc ggt gag gtc agt gtt ggg ttt gag 643

Ala Pro Asp Ser Ser Val Pro Val Gly Glu Val Ser Val Gly Phe Glu

170 175 180

cga gac agt gtg tat tcc cgc ctg ccc atg ttc ctc gcc gcc ctt gct 691

Arg Asp Ser Val Tyr Ser Arg Leu Pro Met Phe Leu Ala Ala Leu Ala

185 190 195

ctt att tct gtg ttg gga atc ctt atc ggc gtg ggt gta gcc atg ggc 739

Leu Ile Ser Val Leu Gly Ile Leu Ile Gly Val Gly Val Ala Met Gly

200 205 210

atg cga cgc cgt tgg gaa cgc gtg acc ttg ggt ttg cag ccg gag gag 787

Met Arg Arg Arg Trp Glu Arg Val Thr Leu Gly Leu Gln Pro Glu Glu

215 220 225

cta gtg acc ctt gtg caa aat cag act gca gtc atc gat ggc att gat 835

Leu Val Thr Leu Val Gln Asn Gln Thr Ala Val Ile Asp Gly Ile Asp

230 235 240 245

gag ggc gtg ctg gcg ctg agc cca aac gga aca att ggg gtg cat aat 883

Glu Gly Val Leu Ala Leu Ser Pro Asn Gly Thr Ile Gly Val His Asn

250 255 260

gag cag gcg caa tcc atg att ggt gca ggt cct atg agt ggc agg acg 931

Glu Gln Ala Gln Ser Met Ile Gly Ala Gly Pro Met Ser Gly Arg Thr

265 270 275

ttg aaa gaa cta ggg ctt gac ctg ggt ctt gat ggc gtt gta ttg cat 979

Leu Lys Glu Leu Gly Leu Asp Leu Gly Leu Asp Gly Val Val Leu His

280 285 290

ggt cag cat ccg gaa acc gtt gcc cat aac ggc agg atc ctc tat ctg 1027

Gly Gln His Pro Glu Thr Val Ala His Asn Gly Arg Ile Leu Tyr Leu

295 300 305

gat ttc cac ccc gtg cgc cgt ggg gat caa gat tta ggc tac gtg gta 1075

Asp Phe His Pro Val Arg Arg Gly Asp Gln Asp Leu Gly Tyr Val Val

310 315 320 325

acc atc cgc gat cgt acc gac atc att gaa ctc agt gaa cgc ctc gac 1123

Thr Ile Arg Asp Arg Thr Asp Ile Ile Glu Leu Ser Glu Arg Leu Asp

330 335 340

tct gtg cgc acc atg acc cac gca ctc cgc gcc cag cgc cac gag ttt 1171

Ser Val Arg Thr Met Thr His Ala Leu Arg Ala Gln Arg His Glu Phe

345 350 355

gcc aac cgc atc cac acc gca aca ggg ctt atc gac gcc ggc cgc gtc 1219

Ala Asn Arg Ile His Thr Ala Thr Gly Leu Ile Asp Ala Gly Arg Val

360 365 370

cac gac gcg gca gag ttt cta ggc gat ata tcc cgc aac ggg gga cag 1267

His Asp Ala Ala Glu Phe Leu Gly Asp Ile Ser Arg Asn Gly Gly Gln

375 380 385

tca cat cca ttg atc gga tca gcg cac ctc aat gaa gca ttt ttg agc 1315

Ser His Pro Leu Ile GIy Ser Ala His Leu Asn Glu Ala Phe Leu Ser

390 395 400 405

tca ttt tta agt act gct tct att tcg gca tct gaa aag ggc gtt agt 1363

Ser Phe Leu Ser Thr Ala Ser Ile Ser Ala Ser Glu Lys Gly Val Ser

410 415 420

ctg cgc atc aac tct gac acg ctc atc ctt ggc act gtt aaa gat cca 1411

Leu Arg Ile Asn Ser Asp Thr Leu Ile Leu Gly Thr Val Lys Asp Pro

425 430 435

gaa gat gta gca acc att ttg ggt aat tta atc aac aat gcc atc gac 1459

Glu Asp Val Ala Thr Ile Leu Gly Asn Leu Ile Asn Asn Ala Ile Asp

440 445 450

gcc gcg gtg gca ggt gaa gcc cca cgg tgg att gag ctt acg ttg atg 1507

Ala Ala Val Ala Gly Glu Ala Pro Arg Trp Ile Glu Leu Thr Leu Met

455 460 465

gat gat gcc gat acg ctg gtc att tct gtt gca gat tct ggt cct gga 1555

Asp Asp Ala Asp Thr Leu Val Ile Ser Val Ala Asp Ser Gly Pro Gly

470 475 480 485

atc cca gag ggc gtg gat gta ttt gcc aca gcc acc cag ata gga gac 1603

Ile Pro Glu Gly Val Asp Val Phe Ala Thr Ala Thr Gln Ile Gly Asp

490 495 500

tct gaa gat aat gaa cgc acc cac ggg cat ggc att ggt cta aaa ctg 1651

Ser Glu Asp Asn Glu Arg Thr His Gly His Gly Ile Gly Leu Lys Leu

505 510 515

tgc cgg gct ttg gct aga tca cat ggt ggc gat gtc tgg gtg att gat 1699

Cys Arg Ala Leu Ala Arg Ser His Gly Gly Asp Val Trp Val Ile Asp

520 525 530

aga gga acc gaa gat ggc gct gta ttt gga gtg aaa cta ccg gga gta 1747

Arg Gly Thr Glu Asp Gly Ala Val Phe Gly Val Lys Leu Pro Gly Val

535 540 545

atg gag taatggatca aacacttaaa gttttagtaa 1783

Met Glu

550

<210>6

<211>551

<212>PRT

<213>谷氨酸棒状杆菌

<400>6

Met Ser Val Gly Gly Ser Asp Trp Lys Asn Phe Lys Glu Val Asp Ile

I 5 10 15

Ile Arg Phe Ala Thr Arg Ile Leu Val Ile Gln Val Ala Thr Val Ala

20 25 30

Leu Val Val Ala Ile Cys Thr Gly Ile Phe Ala Val Leu Met Met Asp

35 40 45

Gln Met Lys Thr Glu Ala Glu His Thr Ala Leu Ser Ile Gly Arg Ser

50 55 60

Val Ala Ser Asn Pro Gln Ile Arg Glu Glu Val Ala Leu Asp Thr Gln

65 70 75 80

Thr Gly Ala Asn Pro Ser Ala Glu Glu Leu Ala Asp Gly Asp Ile Gln

85 90 95

Ala Val Ala Gln Ala Ala Asn Glu Arg Thr Gly Ala Leu Phe Val Val

100 105 110

Ile Thr Asp Gly Leu Gly Ile Arg Leu Ser His Pro Asp Glu Glu Arg

115 120 125

Leu Gly Glu Gln Val Ser Thr Ser Phe Glu Ala Ala Met Arg Gly Glu

130 135 140

Glu Thr Met Ala Trp Glu Thr Gly Thr Leu Gly Ala Ser Ala Arg Ala

145 150 155 160

Lys Val Pro Ile Phe Ala Pro Asp Ser Ser Val Pro Val Gly Glu Val

165 170 175

Ser Val Gly Phe Glu Arg Asp Ser Val Tyr Ser Arg Leu Pro Met Phe

180 185 190

Leu Ala Ala Leu Ala Leu Ile Ser Val Leu Gly Ile Leu Ile Gly Val

195 200 205

Gly Val Ala Met Gly Met Arg Arg Arg Trp Glu Arg Val Thr Leu Gly

210 215 220

Leu Gln Pro Glu Glu Leu Val Thr Leu Val Gln Asn Gln Thr Ala Val

225 230 235 240

Ile Asp Gly Ile Asp Glu Gly Val Leu Ala Leu Ser Pro Asn Gly Thr

245 250 255

Ile Gly Val His Asn Glu Gln Ala Gln Ser Met Ile Gly Ala Gly Pro

260 265 270

Met Ser Gly Arg Thr Leu Lys Glu Leu Gly Leu Asp Leu Gly Leu Asp

275 280 285

Gly Val Val Leu His Gly Gln His Pro Glu Thr Val Ala His Asn Gly

290 295 300

Arg Ile Leu Tyr Leu Asp Phe His Pro Val Arg Arg Gly Asp Gln Asp

305 310 315 320

Leu Gly Tyr Val Val Thr Ile Arg Asp Arg Thr Asp Ile Ile Glu Leu

325 330 335

Ser Glu Arg Leu Asp Ser Val Arg Thr Met Thr His Ala Leu Arg Ala

340 345 350

Gln Arg His Glu Phe Ala Asn Arg Ile His Thr Ala Thr Gly Leu Ile

355 360 365

Asp Ala Gly Arg Val His Asp Ala Ala Glu Phe Leu Gly Asp Ile Ser

370 375 380

Arg Asn Gly Gly Gln Ser His Pro Leu Ile Gly Ser Ala His Leu Asn

385 390 395 400

Glu Ala Phe Leu Ser Ser Phe Leu Ser Thr Ala Ser Ile Ser Ala Ser

405 410 415

Glu Lys Gly Val Ser Leu Arg Ile Asn Ser Asp Thr Leu Ile Leu Gly

420 425 430

Thr Val Lys Asp Pro Glu Asp Val Ala Thr Ile Leu Gly Asn Leu Ile

435 440 445

Asn Asn Ala Ile Asp Ala Ala Val Ala Gly Glu Ala Pro Arg Trp Ile

450 455 460

Glu Leu Thr Leu Met Asp Asp Ala Asp Thr Leu Val Ile Ser Val Ala

465 470 475 480

Asp Ser Gly Pro Gly Ile Pro Glu Gly Val Asp Val Phe Ala Thr Ala

485 490 495

Thr Gln Ile Gly Asp Ser Glu Asp Asn Glu Arg Thr His Gly His Gly

500 505 510

Ile Gly Leu Lys Leu Cys Arg Ala Leu Ala Arg Ser His Gly Gly Asp

515 520 525

Val Trp Val Ile Asp Arg Gly Thr Glu Asp Gly Ala Val Phe Gly Val

530 535 540

Lys Leu Pro Gly Val Met Glu

545 550

<210>7

<211>628

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(598)

<223>RXA00319

<400>7

atcgattgtg ttgccgtaac ctggggctac ggcagcaaaa ctgaatggga cgctgcccgc 60

tacaccgtga gcaccgcaga agaattagaa aggatcatcc atg act ggg cct aaa 115

Met Thr Gly Pro Lys

1 5

act tcg cta cct gtg gaa att gtt ttc gta tgc acc gga aac att tgc 163

Thr Ser Leu Pro Val Glu Ile Val Phe Val Cys Thr Gly Asn Ile Cys

10 15 20

cga tcc ccc atg tcg gaa gtc atc gcg aag gca aaa gcg gaa gaa gct 211

Arg Ser Pro Met Ser Glu Val Ile Ala Lys Ala Lys Ala Glu Glu Ala

25 30 35

ggc ttg gaa gac aac gtc att ttc tcc tcc tgt ggc atg ggc aat tgg 259

Gly Leu Glu Asp Asn Val Ile Phe Ser Ser Cys Gly Met Gly Asn Trp

40 45 50

cac gtt ggc caa cct gct gac aag cga gct ctc gcg gaa ctg aaa tca 307

His Val Gly Gln Pro Ala Asp Lys Arg Ala Leu Ala Glu Leu Lys Ser

55 60 65

gcc ggt tac aac ggc gac acc cac cgc gca gca caa ctt ggt ccc gag 355

Ala Gly Tyr Asn Gly Asp Thr His Arg Ala Ala Gln Leu Gly Pro Glu

70 75 80 85

cac atg cgc gca gat ctc ttc gtc gcg cta gat tcc ggc cac gcc ggt 403

His Met Arg Ala Asp Leu Phe Val Ala Leu Asp Ser Gly His Ala Gly

90 95 100

gag ctc gcc gca acg ggt gtt ccc aac gac aaa atc cgc ctc atg cgt 451

Glu Leu Ala Ala Thr Gly Val Pro Asn Asp Lys Ile Arg Leu Met Arg

105 110 115

tcc ttc gac cca gag tcc aac ccc acc gac gat gtc gca gac cct tac 499

Ser Phe Asp Pro Glu Ser Asn Pro Thr Asp Asp Val Ala Asp Pro Tyr

120 125 130

tac ggc aca tcc cag gat ttc gtg ctc acc cgt gaa aac atc gaa gat 547

Tyr Gly Thr Ser Gln Asp Phe Val Leu Thr Arg Glu Asn Ile Glu Asp

135 140 145

gct atg ccg ggc ctt ttg gag tgg gtc aga gat cac atc cgc act gat 595

Ala Met Pro Gly Leu Leu Glu Trp Val Arg Asp His Ile Arg Thr Asp

150 155 160 165

tct taggtctttg agctaaaaag tcctaaggta 628

Ser

210>8

<211>166

<212>PRT

<213>谷氨酸棒状杆菌

<400>8

Met Thr Gly Pro Lys Thr Ser Leu Pro Val Glu Ile Val Phe Val Cys

1 5 10 15

Thr Gly Asn Ile Cys Arg Ser Pro Met Ser Glu Val Ile Ala Lys Ala

20 25 30

Lys Ala Glu Glu Ala Gly Leu Glu Asp Asn Val Ile Phe Ser Ser Cys

35 40 45

Gly Met Gly Asn Trp His Val Gly Gln Pro Ala Asp Lys Arg Ala Leu

50 55 60

Ala Glu Leu Lys Ser Ala Gly Tyr Asn Gly Asp Thr His Arg Ala Ala

65 70 75 80

Gln Leu Gly Pro Glu His Met Arg Ala Asp Leu Phe Val Ala Leu Asp

85 90 95

Ser Gly His Ala Gly Glu Leu Ala Ala Thr Gly Val Pro Asn Asp Lys

100 105 110

Ile Arg Leu Met Arg Ser Phe Asp Pro Glu Ser Asn Pro Thr Asp Asp

115 120 125

Val Ala Asp Pro Tyr Tyr Gly Thr Ser Gln Asp Phe Val Leu Thr Arg

130 135 140

Glu Asn Ile Glu Asp Ala Met Pro Gly Leu Leu Glu Trp Val Arg Asp

145 150 155 160

His Ile Arg Thr Asp Ser

165

<210>9

<211>1039

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1009)

<223>RXA00486

<400>9

gtttattcat gcccttgatt attgccaaag aaacctttaa ggactagatc gaaaaacagc 60

caactatagt taagtaatac tgaacaattt tggaggtgtc gtg ctc aat ctc aac 115

Val Leu Asn Leu Asn

1 5

cgc tta cac atc ctg cag gaa ttc cac cgc ctg gga acg att aca gca 163

Arg Leu His Ile Leu Gln Glu Phe His Arg Leu Gly Thr Ile Thr Ala

10 15 20

gtg gcg gaa tcc atg aac tac agc cgc tct gcc atc tcc caa caa atg 211

Val Ala Glu Ser Met Asn Tyr Ser Arg Ser Ala Ile Ser Gln Gln Met

25 30 35

gcg ctg ctg gaa aaa gaa att ggt gtg aaa ctc ttt gaa aaa agc ggc 259

Ala Leu Leu Glu Lys Glu Ile Gly Val Lys Leu Phe Glu Lys Ser Gly

40 45 50

cga aac ctc tac ttc aca gaa caa ggc gaa gtg ttg gcc tca gaa aca 307

Arg Asn Leu Tyr Phe Thr Glu Gln Gly Glu Val Leu Ala Ser Glu Thr

55 60 65

cat gcg atc atg gca gca gtc gac cat gcc cgc gca gcc gtt cta gat 355

His Ala Ile Met Ala Ala Val Asp His Ala Arg Ala Ala Val Leu Asp

70 75 80 85

tcg ctg tct gaa gtg tcc gga acg ctg aaa gtc acc tcc ttc caa tcc 403

Ser Leu Ser Glu Val Ser Gly Thr Leu Lys Val Thr Ser Phe Gln Ser

90 95 100

ctg ctg ttc acc ctt gcc ccg aaa gcc atc gcg cgc ctg acc gag aaa 451

Leu Leu Phe Thr Leu Ala Pro Lys Ala Ile Ala Arg Leu Thr Glu Lys

105 110 115

tac cca cac ctg caa gta gaa atc tcc caa cta gaa gtc acc gca gcg 499

Tyr Pro His Leu Gln Val Glu Ile Ser Gln Leu Glu Val Thr Ala Ala

120 125 130

ctc gaa gaa ctc cgc gcc cgc cgc gtc gac gtc gca ctc ggc gag gaa 547

Leu Glu Glu Leu Arg Ala Arg Arg Val Asp Val Ala Leu Gly Glu Glu

135 140 145

tac ccc gtg gaa gtc ccc ctt gtt gag gcc agc att cac cgc gaa gtc 595

Tyr Pro Val Glu Val Pro Leu Val Glu Ala Ser Ile His Arg Glu Val

150 155 160 165

ctc ttc gaa gac ccc atg ctg ctc gtc acc cca gca agc ggc cca tac 643

Leu Phe Glu Asp Pro Met Leu Leu Val Thr Pro Ala Ser Gly Pro Tyr

170 175 180

tct ggc ctc acc ctg cca gaa ctc cgc gac atc ccc atc gcc atc gat 691

Ser Gly Leu Thr Leu Pro Glu Leu Arg Asp Ile Pro Ile Ala Ile Asp

185 190 195

cca ccc gac ctt ccc gcg ggc gaa tgg gtc cat agg ctc tgc cgg cgc 739

Pro Pro Asp Leu Pro Ala Gly Glu Trp Val His Arg Leu Cys Arg Arg

200 205 210

gcc ggg ttt gag ccc cgc gtg acc ttt gaa acc agc gat ccc atg ctc 787

Ala Gly Phe Glu Pro Arg Val Thr Phe Glu Thr Ser Asp Pro Met Leu

215 220 225

caa gca cac ctc gtg cgt agc ggc ttg gcc gtg aca ttt tcc ccc aca 835

Gln Ala His Leu Val Arg Ser Gly Leu Ala Val Thr Phe Ser Pro Thr

230 235 240 245

ctg ctc acc ccg atg ctg gaa agc gtg cac atc cag ccg ctg ccc ggc 883

Leu Leu Thr Pro Met Leu Glu Ser Val His Ile Gln Pro Leu Pro Gly

250 255 260

aac ccc acg cgc acg ctc tac acc gcg gtc agg gaa ggg cgc cag ggg 931

Asn Pro Thr Arg Thr Leu Tyr Thr Ala Val Arg Glu Gly Arg Gln Gly

265 270 275

cat cca gcc att aaa gct ttt cga cga gcc ctc gcc cat gtg gcc aaa979

His Pro Ala Ile Lys Ala Phe Arg Arg Ala Leu Ala His Val Ala Lys

280 285 290

gaa tct tat ttg gag gct cgt cta gta gag tgagttcttg tgagccttca 1029

Glu Ser Tyr Leu Glu Ala Arg Leu Val Glu

295 300

gacaaatcat 1039

<210>10

<211>303

<212>PRT

<213>谷氨酸棒状杆菌

<400>10

Val Leu Asn Leu Asn Arg Leu His Ile Leu Gln Glu Phe His Arg Leu

1 5 10 15

Gly Thr Ile Thr Ala Val Ala Glu Ser Met Asn Tyr Ser Arg Ser Ala

20 25 30

Ile Ser Gln Gln Met Ala Leu Leu Glu Lys Glu Ile Gly Val Lys Leu

35 40 45

Phe Glu Lys Ser Gly Arg Asn Leu Tyr Phe Thr Glu Gln Gly Glu Val

50 55 60

Leu Ala Ser Glu Thr His Ala Ile Met Ala Ala Val Asp His Ala Arg

65 70 75 80

Ala Ala Val Leu Asp Ser Leu Ser Glu Val Ser Gly Thr Leu Lys Val

85 90 95

Thr Ser Phe Gln Ser Leu Leu Phe Thr Leu Ala Pro Lys Ala Ile Ala

100 105 110

Arg Leu Thr Glu Lys Tyr Pro His Leu Gln Val Glu Ile Ser Gln Leu

115 120 125

Glu Val Thr Ala Ala Leu Glu Glu Leu Arg Ala Arg Arg Val Asp Val

130 135 140

Ala Leu Gly Glu Glu Tyr Pro Val Glu Val Pro Leu Val Glu Ala Ser

145 150 155 160

Ile His Arg Glu Val Leu Phe Glu Asp Pro Met Leu Leu Val Thr Pro

165 170 175

Ala Ser Gly Pro Tyr Ser Gly Leu Thr Leu Pro Glu Leu Arg Asp Ile

180 185 190

Pro Ile Ala Ile Asp Pro Pro Asp Leu Pro Ala Gly Glu Trp Val His

195 200 205

Arg Leu Cys Arg Arg Ala Gly Phe Glu Pro Arg Val Thr Phe Glu Thr

210 215 220

Ser Asp Pro Met Leu Gln Ala His Leu Val Arg Ser Gly Leu Ala Val

225 230 235 240

Thr Phe Ser Pro Thr Leu Leu Thr Pro Met Leu Glu Ser Val His Ile

245 250 255

Gln Pro Leu Pro Gly Asn Pro Thr Arg Thr Leu Tyr Thr Ala Val Arg

260 265 270

Glu Gly Arg Gln Gly His Pro Ala Ile Lys Ala Phe Arg Arg Ala Leu

275 280 285

Ala His Val Ala Lys Glu Ser Tyr Leu Glu Ala Arg Leu Val Glu

290 295 300

<210>11

<211>478

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(448)

<223>RXA00593

<400>11

ttggtgtgat gcattcgaca gcaaattggc tgtgtgacta cacttgcgag tgtattaagt 60

attaggccgt gcatatgtag cgcattttaa ggagattgtc atg acg tct gtg att 115

Met Thr Ser Val Ile

1 5

cca gag cag cgc aac aac ccc ttt tat agg gac agc gcc aca att gct 163

Pro Glu Gln Arg Asn Asn Pro Phe Tyr Arg Asp Ser Ala Thr Ile Ala

10 15 20

tcc tcg gac cac aca gag cgt ggt gag tgg gtc act cag gca aag tgt 211

Ser Ser Asp His Thr Glu Arg Gly Glu Trp Val Thr Gln Ala Lys Cys

25 30 35

cga aat ggc gac cca gat gca ttg ttt gtt cgt ggt gca gcg caa cgc 259

Arg Asn Gly Asp Pro Asp Ala Leu Phe Val Arg Gly Ala Ala Gln Arg

40 45 50

cga gca gca gca att tgc cgc cac tgc cct gta gcc atg cag tgc tgc 307

Arg Ala Ala Ala Ile Cys Arg His Cys Pro Val Ala Met Gln Cys Cys

55 60 65

gcc gat gcc tta gat aac aag gtg gaa ttc gga gtc tgg gga ggc ctg 355

Ala Asp Ala Leu Asp Asn Lys Val Glu Phe Gly Val Trp Gly Gly Leu

70 75 80 85

acc gag cgc cag cgc cgt gca ttg ctt cga aag aag ccg cac att act 403

Thr Glu Arg Gln Arg Arg Ala Leu Leu Arg Lys Lys Pro His Ile Thr

90 95 100

aac tgg gct gaa tat ttg gct cag ggg ggc gag atc gcc ggg gtt 448

Asn Trp Ala Glu Tyr Leu Ala Gln Gly Gly Glu Ile Ala Gly Val

105 110 115

taattaattt caagggctgg ccattaacgt 478

<210>12

<211>116

<212>PRT

<213>谷氨酸棒状杆菌

<400>12

Met Thr Ser Val Ile Pro Glu Gln Arg Asn Asn Pro Phe Tyr Arg Asp

1 5 10 15

Ser Ala Thr Ile Ala Ser Ser Asp His Thr Glu Arg Gly Glu Trp Val

20 25 30

Thr Gln Ala Lys Cys Arg Asn Gly Asp Pro Asp Ala Leu Phe Val Arg

35 40 45

Gly Ala Ala Gln Arg Arg Ala Ala Ala Ile Cys Arg His Cys Pro Val

50 55 60

Ala Met Gln Cys Cys Ala Asp Ala Leu Asp Asn Lys Val Glu Phe Gly

65 70 75 80

Val Trp Gly Gly Leu Thr Glu Arg Gln Arg Arg Ala Leu Leu Arg Lys

85 90 95

Lys Pro His Ile Thr Asn Trp Ala Glu Tyr Leu Ala Gln Gly Gly Glu

100 105 110

Ile Ala Gly Val

115

<210>13

<211>796

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101..(766)

<223>RXA00609

<400>13

gaagcccacg gcggccgagc tttcgtcaat tccacaccag gtctaggatc cattttcggc 60

ctggaaatcc ccgcaccaga acaatcaaag gaatacaccc atg agc aag atc ctg l15

Met Ser Lys Ile Leu

1 5

ctc gct gaa gat gac gcc ggc atc gca gat ttc atc gtt cgt ggc ctc 163

Leu Ala Glu Asp Asp Ala Gly Ile Ala Asp Phe Ile Val Arg Gly Leu

10 15 20

atc cgc gaa ggc ttc gaa tgc gag gtc acc gaa tcc ggc gcc gaa gct 211

Ile Arg Glu Gly Phe Glu Cys Glu Val Thr Glu Ser Gly Ala Glu Ala

25 30 35

ttc gcc cgc gca cat tcc ggc gat ttc gat ctc atg gtt tta gac ctc 259

Phe Ala Arg Ala His Ser Gly Asp Phe Asp Leu Met Val Leu Asp Leu

40 45 50

ggc ctc ccc cac atg gac ggc acg gat gtc cta gag caa tta aga aat 307

Gly Leu Pro His Met Asp Gly Thr Asp Val Leu Glu Gln Leu Arg Asn

55 60 65

ctg cag gtc acg cta cct atc att gtg ctc acg gca cgc acc aac atc 355

Leu Gln Val Thr Leu Pro Ile Ile Val Leu Thr Ala Arg Thr Asn Ile

70 75 80 85

gag gac cgc ctc cgc acc ctc gag ggc ggc gcc gac gat tac atg ccc 403

Glu Asp Arg Leu Arg Thr Leu Glu Gly Gly Ala Asp Asp Tyr Met Pro

90 95 100

aaa cca ttc caa ttc gca gaa tta ctg gcc cgc atc aaa ctc cgc ctc 451

Lys Pro Phe Gln Phe Ala Glu Leu Leu Ala Arg Ile Lys Leu Arg Leu

105 110 115

gcc aaa cac act cct cag gaa acg ccg acc gat gcg cgc gtg cta cga 499

Ala Lys His Thr Pro Gln Glu Thr Pro Thr Asp Ala Arg Val Leu Arg

120 125 130

aac ggc gat ttg gag ctc gat ctt cgt acc cag cgt gtg ctc atc gac 547

Asn Gly Asp Leu Glu Leu Asp Leu Arg Thr Gln Arg Val Leu Ile Asp

135 140 145

ggc tcc tgg cac gac ctt tcc cgc cgc gaa gtc gat ctg ctc gaa acc 595

Gly Ser Trp His Asp Leu Ser Arg Arg Glu Val Asp Leu Leu Glu Thr

150 155 160 165

ctc atg cga cac cca ggg caa atc ctc tcc cga gtc caa ctc ctc cga 643

Leu Met Arg His Pro Gly Gln Ile Leu Ser Arg Val Gln Leu Leu Arg

170 175 180

ctg gtg tgg gac atg gat tgg gac ccc ggc tca aac gtg gtg gac gta 691

Leu Val Trp Asp Met Asp Trp Asp Pro Gly Ser Asn Val Val Asp Val

185 190 195

tat atc cgc gcg ttg agg aag aaa atc ggt gcc cat cgg gtc gaa acc 739

Tyr Ile Arg Ala Leu Arg Lys Lys Ile Gly Ala His Arg Val Glu Thr

200 205 210

atc cga gga tct ggc tac cgg ctg cgc taactgcaga acgagaccaa 786

Ile Arg Gly Ser Gly Tyr Arg Leu Arg

215 220

aaacctaaaa 796

<210>14

<211>222

<212>PRT

<213>谷氨酸棒状杆菌

<400>14

Met Ser Lys Ile Leu Leu Ala Glu Asp Asp Ala Gly Ile Ala Asp Phe

1 5 10 15

Ile Val Arg Gly Leu Ile Arg Glu Gly Phe Glu Cys Glu Val Thr Glu

20 25 30

Ser Gly Ala Glu Ala Phe Ala Arg Ala His Ser Gly Asp Phe Asp Leu

35 40 45

Met Val Leu Asp Leu Gly Leu Pro His Met Asp Gly Thr Asp Val Leu

50 55 60

Glu Gln Leu Arg Asn Leu Gln Val Thr Leu Pro Ile Ile Val Leu Thr

65 70 75 80

Ala Arg Thr Asn Ile Glu Asp Arg Leu Arg Thr Leu Glu Gly Gly Ala

85 90 95

Asp Asp Tyr Met Pro Lys Pro Phe Gln Phe Ala Glu Leu Leu Ala Arg

. 100 105 110

Ile Lys Leu Arg Leu Ala Lys His Thr Pro Gln Glu Thr Pro Thr Asp

115 120 125

Ala Arg Val Leu Arg Asn Gly Asp Leu Glu Leu Asp Leu Arg Thr Gln

130 135 140

Arg Val Leu Ile Asp Gly Ser Trp His Asp Leu Ser Arg Arg Glu Val

145 150 155 160

Asp Leu Leu Glu Thr Leu Met Arg His Pro Gly Gln Ile Leu Ser Arg

165 170 175

Val Gln Leu Leu Arg Leu Val Trp Asp Met Asp Trp Asp Pro Gly Ser

180 185 190

Asn Val Val Asp Val Tyr Ile Arg Ala Leu Arg Lys Lys Ile Gly Ala

195 200 205

His Arg Val Glu Thr Ile Arg Gly Ser Gly Tyr Arg Leu Arg

210 215 220

<210>15

<211>1498

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1468)

<223>RXA00651

<400>15

cgcgtcgatg ggaaggccgt cagtaattac ttccggggct gcctcggtgg tggtctctgg 60

ggttgcttca ggttccgccg gggtacaagc ggtgagcatg atg gaa gca gcg agg 115

Met Glu Ala Ala Arg

1 5

ata gta ggt aat gta cga cgc atg cag tca agc cta gat cgt gtg tcg 163

Ile Val Gly Asn Val Arg Arg Met Gln Ser Ser Leu Asp Arg Val Ser

10 15 20

gaa acc gga cgc aat gag ctc gat gtt gaa acc ctt gtg aag aag ggg 211

Glu Thr Gly Arg Asn Glu Leu Asp Val Glu Thr Leu Val Lys Lys Gly

25 30 35

aat caa ccg ggc gcg atg agc tat cgc aac agt atc cac att ttg aca 259

Asn Gln Pro Gly Ala Met Ser Tyr Arg Asn Ser Ile His Ile Leu Thr

40 45 50

gcc tcg ctg ctg gtc gtg ggg ttg gga gct tcc gcc cgc ctg acg ctg 307

Ala Ser Leu Leu Val Val Gly Leu Gly Ala Ser Ala Arg Leu Thr Leu

55 60 65

ccg atg ttt gcg ctg tcg tgc gtg ctg ttg ttt gtg tgg ggt ttt ctg 355

Pro Met Phe Ala Leu Ser Cys Val Leu Leu Phe Val Trp Gly Phe Leu

70 75 80 85

tac ttc tat gga tca acc aaa cgc gta gat ttg agc cac ggc atg cag 403

Tyr Phe Tyr Gly Ser Thr Lys Arg Val Asp Leu Ser His Gly Met Gln

90 95 100

ctg ggc tgg ctg ttt gtg ctg acg ctg gtg tgg att ttt atg gtg ccg 451

Leu Gly Trp Leu Phe Val Leu Thr Leu Val Trp Ile Phe Met Val Pro

105 110 115

atc gtg ccc gtg tcc att tat ctg ctg ttc ccg ctg ttt ttc ctc tat 499

Ile Val Pro Val Ser Ile Tyr Leu Leu Phe Pro Leu Phe Phe Leu Tyr

120 125 130

cta cag gtg atg cct gac gtg aga ggc att att gcg att ttg ggt gcg 547

Leu Gln Val Met Pro Asp Val Arg Gly Ile Ile Ala Ile Leu Gly Ala

135 140 145

aca gcg att gcg att gcc agc cag tat tcc gtg ggg ttg acc ttt ggt 595

Thr Ala Ile Ala Ile Ala Ser Gln Tyr Ser Val Gly Leu Thr Phe Gly

150 155 160 165

ggt gtg atg ggt ccg gtg gtc tct gcg atc gtg acc gtg gct att gat 643

Gly Val Met Gly Pro Val Val Ser Ala Ile Val Thr Val Ala Ile Asp

170 175 180

tac gcg ttc cgc acg ttg tgg cgg gtg aat aat gaa aag cag gaa ttg 691

Tyr Ala Phe Arg Thr Leu Trp Arg Val Asn Asn Glu Lys Gln Glu Leu

185 190 195

att gat cag ttg att gaa act cgc tcc cag ctg gcg gtg acg gaa cga 739

Ile Asp Gln Leu Ile Glu Thr Arg Ser Gln Leu Ala Val Thr Glu Arg

200 205 210

aat gcg ggt att gct gcg gaa cgt caa cgt att gcg cat gaa att cat 787

Asn Ala Gly Ile Ala Ala Glu Arg Gln Arg Ile Ala His Glu Ile His

215 220 225

gac acg gtc gcc cag gga ctc tcc tcc att caa atg ctg ctg cat gtc 835

Asp Thr Val Ala Gln Gly Leu Ser Ser Ile Gln Met Leu Leu His Val

230 235 240 245

tct gaa cag gag att ctc gtt gct gag atg gaa gag aag cca aag gag 883

Ser Glu Gln Glu Ile Leu Val Ala Glu Met Glu Glu Lys Pro Lys Glu

250 255 260

gcg atc gtg aag aag atg cgc ctt gcc cga caa aca gcc tcc gac aat 931

Ala Ile Val Lys Lys Met Arg Leu Ala Arg Gln Thr Ala Ser Asp Asn

265 270 275

ctc agt gag gct cgc gcg atg att gcg gcg ttg caa ccg gca gcg ctg 979

Leu Ser Glu Ala Arg Ala Met Ile Ala Ala Leu Gln Pro Ala Ala Leu

280 285 290

tct aaa acc tcc ttg gaa gca gca ctt cac cgc gtc aca gaa ccg ttg 1027

Ser Lys Thr Ser Leu Glu Ala Ala Leu His Arg Val Thr Glu Pro Leu

295 300 305

ttg ggt att aat ttt gtg att tct gtc gac ggt gat gtt cgc caa ctg 1075

Leu Gly Ile Asn Phe Val Ile Ser Val Asp Gly Asp Val Arg Gln Leu

310 315 320 325

ccc atg aaa act gaa gcc acc ctt ctg cga att gct caa ggt gcg atc 1123

Pro Met Lys Thr Glu Ala Thr Leu Leu Arg Ile Ala Gln Gly Ala Ile

330 335 340

gga aat gtg gcg aaa cat tca gag gcg aaa aac tgc cac gtg aca cta 1171

Gly Asn Val Ala Lys His Ser Glu Ala Lys Asn Cys His Val Thr Leu

345 350 355

acc tac gaa gac aca gaa gta cgc ctt gat gtg gtt gat gac ggt gtg 1219

Thr Tyr Glu Asp Thr Glu Val Arg Leu Asp Val Val Asp Asp Gly Val

360 365 370

ggt ttt gag cct tcg gaa gtg tcc agt acc ccc gct ggc ctt ggc cat 1267

Gly Phe Glu Pro Ser Glu Val Ser Ser Thr Pro Ala Gly Leu Gly His

375 380 385

atc ggc tta acc gca ttg cag cag cgt gcg atg gaa ttg cac ggc gaa 1315

Ile Gly Leu Thr Ala Leu Gln Gln Arg Ala Met Glu Leu His Gly Glu

390 395 400 405

gtt ata gtg gaa tct gca tat ggg cag ggt act gcg gta tct gca gca 1363

Val Ile Val Glu Ser Ala Tyr Gly Gln Gly Thr Ala Val Ser Ala Ala

410 415 420

ttg ccg gtg gag cca cca gag ggg ttt gtc ggg gcg ccg gtt ttg gca 1411

Leu Pro Val Glu Pro Pro Glu Gly Phe Val Gly Ala Pro Val Leu Ala

425 430 435

gat tcg gac tca agt gct aca ggc gag gtt gaa cta agt tct cca act 1459

Asp Ser Asp Ser Ser Ala Thr Gly Glu Val Glu Leu Ser Ser Pro Thr

440 445 450

gac gat gag taaggctaga ctaaagtacg attcatctgc 1498

Asp Asp Glu

455

<210>16

<211>456

<212>PRT

<213>谷氨酸棒状杆菌

<400>16

Met Glu Ala Ala Arg Ile Val Gly Asn Val Arg Arg Met Gln Ser Ser

1 5 10 15

Leu Asp Arg Val Ser Glu Thr Gly Arg Asn Glu Leu Asp Val Glu Thr

20 25 30

Leu Val Lys Lys Gly Asn Gln Pro Gly Ala Met Ser Tyr Arg Asn Ser

35 40 45

Ile His Ile Leu Thr Ala Ser Leu Leu Val Val Gly Leu Gly Ala Ser

50 55 60

Ala Arg Leu Thr Leu Pro Met Phe Ala Leu Ser Cys Val Leu Leu Phe

65 70 75 80

Val Trp Gly Phe Leu Tyr Phe Tyr Gly Ser Thr Lys Arg Val Asp Leu

85 90 95

Ser His Gly Met Gln Leu Gly Trp Leu Phe Val Leu Thr Leu Val Trp

100 105 110

Ile Phe Met Val Pro Ile Val Pro Val Ser Ile Tyr Leu Leu Phe Pro

115 120 125

Leu Phe Phe Leu Tyr Leu Gln Val Met Pro Asp Val Arg Gly Ile Ile

130 135 140

Ala Ile Leu Gly Ala Thr Ala Ile Ala Ile Ala Ser Gln Tyr Ser Val

145 150 155 160

Gly Leu Thr Phe Gly Gly Val Met Gly Pro Val Val Ser Ala Ile Val

165 170 175

Thr Val Ala Ile Asp Tyr Ala Phe Arg Thr Leu Trp Arg Val Asn Asn

180 185 190

Glu Lys Gln Glu Leu Ile Asp Gln Leu Ile Glu Thr Arg Ser Gln Leu

195 200 205

Ala Val Thr Glu Arg Asn Ala Gly Ile Ala Ala Glu Arg Gln Arg Ile

210 215 220

Ala His Glu Ile His Asp Thr Val Ala Gln Gly Leu Ser Ser Ile Gln

225 230 235 240

Met Leu Leu His Val Ser Glu Gln Glu Ile Leu Val Ala Glu Met Glu

245 250 255

Glu Lys Pro Lys Glu Ala Ile Val Lys Lys Met Arg Leu Ala Arg Gln

260 265 270

Thr Ala Ser Asp Asn Leu Ser Glu Ala Arg Ala Met Ile Ala Ala Leu

275 280 285

Gln Pro Ala Ala Leu Ser Lys Thr Ser Leu Glu Ala Ala Leu His Arg

290 295 300

Val Thr Glu Pro Leu Leu Gly Ile Asn Phe Val Ile Ser Val Asp Gly

305 310 315 320

Asp Val Arg Gln Leu Pro Met Lys Thr Glu Ala Thr Leu Leu Arg Ile

325 330 335

Ala Gln Gly Ala Ile Gly Asn Val Ala Lys His Ser Glu Ala Lys Asn

340 345 350

Cys His Val Thr Leu Thr Tyr Glu Asp Thr Glu Val Arg Leu Asp Val

355 360 365

Val Asp Asp Gly Val Gly Phe Glu Pro Ser Glu Val Ser Ser Thr Pro

370 375 380

Ala Gly Leu Gly His Ile Gly Leu Thr Ala Leu Gln Gln Arg Ala Met

385 390 395 400

Glu Leu His Gly Glu Val Ile Val Glu Ser Ala Tyr Gly Gln Gly Thr

405 410 415

Ala Val Ser Ala Ala Leu Pro Val Glu Pro Pro Glu Gly Phe Val Gly

420 425 430

Ala Pro Val Leu Ala Asp Ser Asp Ser Ser Ala Thr Gly Glu Val Glu

435 440 445

Leu Ser Ser Pro Thr Asp Asp Glu

450 455

<210>17

<211>1759

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1729)

<223>RXA00719

<400>17

cagatgatgc acacatcgtg gacacctctg atatgaccat ggatcaagta cttgatcacc 60

tcatccacct agtggaagcc tccgctgaaa ggagcaacca gtg act gat aaa cac 115

Val Thr Asp Lys His

l 5

acc atg cct ggt gaa gag gac gac acc gta ttc gtc tac cac acc cac 163

Thr Met Pro Gly Glu Glu Asp Asp Thr Val Phe Val Tyr His Thr His

10 15 20

aaa ggc gaa atg gac gtc gaa ggt gcg ttt gct gac gaa gaa gaa cta 211

Lys Gly Glu Met Asp Val Glu Gly Ala Phe Ala Asp Glu Glu Glu Leu

25 30 35

gca cca cac ggc ggt tgg gct tcc gca gat ttc gac cca gca gaa ttc 259

Ala Pro His Gly Gly Trp Ala Ser Ala Asp Phe Asp Pro Ala Glu Phe

40 45 50

ggc tac gaa gac tct gac gat gac ttc gat gca gag gac ttt gac gaa 307

Gly Tyr Glu Asp Ser Asp Asp Asp Phe Asp Ala Glu Asp Phe Asp Glu

55 60 65

aca gag ttc tcc aac cct gat ttc ggc gaa gac tac tct gat gaa gac 355

Thr Glu Phe Ser Asn Pro Asp Phe Gly Glu Asp Tyr Ser Asp Glu Asp

70 75 80 85

tgg gaa gaa atc gag acc gca ttc gga ttc gac cca agc cac ctt gaa 403

Trp Glu Glu Ile Glu Thr Ala Phe Gly Phe Asp Pro Ser His Leu Glu

90 95 100

gaa gct ctc tgc acg gtc gct atc gtc gga cgc cca aat gtt ggt aaa 451

Glu Ala Leu Cys Thr Val Ala Ile Val Gly Arg Pro Asn Val Gly Lys

105 110 115

tca acc ttg gtg aac cgc ttt att gga cgt cga gaa gca gtc gtg gaa 499

Ser Thr Leu Val Asn Arg Phe Ile Gly Arg Arg Glu Ala Val Val Glu

120 125 130

gat ttc ccc ggc gta acc cgt gac cgc atc tcc tac atc tct gac tgg 547

Asp Phe Pro Gly Val Thr Arg Asp Arg Ile Ser Tyr Ile Ser Asp Trp

135 140 145

ggt gga cac cgt ttc tgg gtt cag gac aca ggc gga tgg gat cct aac 595

Gly Gly His Arg Phe Trp Val Gln Asp Thr Gly Gly Trp Asp Pro Asn

150 155 160 165

gtc aag ggc atc cac gca tcg atc gca cag caa gca gaa gtt gct atg 643

Val Lys Gly Ile His Ala Ser Ile Ala Gln Gln Ala Glu Val Ala Met

170 175 180

agc act gcc gat gtc atc gta ttc gtc gtg gac acc aag gtg ggc atc 691

Ser Thr Ala Asp Val Ile Val Phe Val Val Asp Thr Lys Val Gly Ile

185 190 195

acc gaa act gac tca gtg atg gca gca aaa ctg ttg cgc tcg gaa gtg 739

Thr Glu Thr Asp Ser Val Met Ala Ala Lys Leu Leu Arg Ser Glu Val

200 205 210

cca gtg atc ttg gtt gcg aac aaa ttc gac tcc gac agc cag tgg gct 787

Pro Val Ile Leu Val Ala Asn Lys Phe Asp Ser Asp Ser Gln Trp Ala

215 220 225

gac atg gct gag ttc tac agc ctc ggc ctt ggc gat cca tac cca gtt 835

Asp Met Ala Glu Phe Tyr Ser Leu Gly Leu Gly Asp Pro Tyr Pro Val

230 235 240 245

tca gcc cag cat gga cgt ggt ggc gct gac gtt ttg gac aaa gtc ctt 883

Ser Ala Gln His Gly Arg Gly Gly Ala Asp Val Leu Asp Lys Val Leu

250 255 260

gaa ctc ttc cca gaa gag cct cgc tcc aag tcc atc gtg gaa ggc cct 931

Glu Leu Phe Pro Glu Glu Pro Arg Ser Lys Ser Ile Val Glu Gly Pro

265 270 275

cgt cgt gtc gcc ctt gtg ggt aag cca aac gtg ggt aag tct tca ctg 979

Arg Arg Val Ala Leu Val Gly Lys Pro Asn Val Gly Lys Ser Ser Leu

280 285 290

ctc aac aag ttt gct ggc gag acc cgc tct gtc gtg gac aat gtt gca 1027

Leu Asn Lys Phe Ala Gly Glu Thr Arg Ser Val Val Asp Asn Val Ala

295 300 305

gga acc acc gtt gac ccc gtt gac tcc ctg att cag ctg gat caa aaa 1075

Gly Thr Thr Val Asp Pro Val Asp Ser Leu Ile Gln Leu Asp GIn Lys

310 315 320 325

ctg tgg aaa ttc gtg gat act gct ggt ctt cgc aaa aag gtc aag act 1123

Leu Trp Lys Phe Val Asp Thr Ala Gly Leu Arg Lys Lys Val Lys Thr

330 335 340

gca tct ggc cac gag tac tac gca tca ctg cgt acc cac ggt gcc atc 1171

Ala Ser Gly His Glu Tyr Tyr Ala Ser Leu Arg Thr His Gly Ala Ile

345 350 355

gat gca gct gag ctg tgt gtt ttg ctt atc gat tcc tcc gaa ccc atc 1219

Asp Ala Ala Glu Leu Cys Val Leu Leu Ile Asp Ser Ser Glu Pro Ile

360 365 370

acc gag cag gat cag cgc gtg ctc gca atg atc acc gat gcc ggt aag 1267

Thr Glu Gln Asp Gln Arg Val Leu Ala Met Ile Thr Asp Ala Gly Lys

375 380 385

gca ctg gtt att gcg ttc aac aag tgg gat ctc atg gat gaa gat cgc 1315

Ala Leu Val Ile Ala Phe Asn Lys Trp Asp Leu Met Asp Glu Asp Arg

390 395 400 405

cgc atc gat ttg gat cgc gaa ctt gat ctc cag ttg gca cac gtg cct 1363

Arg Ile Asp Leu Asp Arg Glu Leu Asp Leu Gln Leu Ala His Val Pro

410 415 420

tgg gca aag cgc atc aac atc tcc gcc aaa acc ggt cgt gca ctg cag 1411

Trp Ala Lys Arg Ile Asn Ile Ser Ala Lys Thr Gly Arg Ala Leu Gln

425 430 435

cgc ctc gag cca gca atg ttg gaa gcg ctc gac aac tgg gat cgc cgt 1459

Arg Leu Glu Pro Ala Met Leu Glu Ala Leu Asp Asn Trp Asp Arg Arg

440 445 450

atc tcc act ggt cag ctg aac acc tgg ctg cgt gaa gca att gct gcg 1507

Ile Ser Thr Gly Gln Leu Asn Thr Trp Leu Arg Glu Ala Ile Ala Ala

455 460 465

aac cca cca cca atg cgt ggc gga cgt ttg cct cga gtg ctg ttt gcc 1555

Asn Pro Pro Pro Met Arg Gly Gly Arg Leu Pro Arg Val Leu Phe Ala

470 475 480 485

acc cag gca tct act cag cca cca gtg atc gta ctg ttc acc acc ggc 1603

Thr Gln Ala Ser Thr Gln Pro Pro Val Ile Val Leu Phe Thr Thr Gly

490 495 500

ttc ctc gaa gca ggt tac cga cga tac ctg gag cgc aag ttc cgt gaa 1651

Phe Leu Glu Ala Gly Tyr Arg Arg Tyr Leu Glu Arg Lys Phe Arg Glu

505 510 515

cgt ttc ggc ttt gaa ggc act cca gtg cga atc gct gtg cgt gtt cgc 1699

Arg Phe Gly Phe Glu Gly Thr Pro Val Arg Ile Ala Val Arg Val Arg

520 525 530

gag cgc cgc ggc aag ggc gga aac aag cag taaagcttga ttttccctaa 1749

Glu Arg Arg Gly Lys Gly Gly Asn Lys Gln

535 540

aagcacttat 1759

<210>18

<211>543

<212>PRT

<213>谷氨酸棒状杆菌

<400>18

Val Thr Asp Lys His Thr Met Pro Gly Glu Glu Asp Asp Thr Val Phe

1 5 10 15

Val Tyr His Thr His Lys Gly Glu Met Asp Val Glu Gly Ala Phe Ala

20 25 30

Asp Glu Glu Glu Leu Ala Pro His Gly Gly Trp Ala Ser Ala Asp Phe

35 40 45

Asp Pro Ala Glu Phe Gly Tyr Glu Asp Ser Asp Asp Asp Phe Asp Ala

50 55 60

Glu Asp Phe Asp Glu Thr Glu Phe Ser Asn Pro Asp Phe Gly Glu Asp

65 70 75 80

Tyr Ser Asp Glu Asp Trp Glu Glu Ile Glu Thr Ala Phe Gly Phe Asp

85 90 95

Pro Ser His Leu Glu Glu Ala Leu Cys Thr Val Ala Ile Val Gly Arg

100 105 110

Pro Asn Val Gly Lys Ser Thr Leu Val Asn Arg Phe Ile Gly Arg Arg

115 120 125

Glu Ala Val Val Glu Asp Phe Pro Gly Val Thr Arg Asp Arg Ile Ser

130 135 140

Tyr Ile Ser Asp Trp Gly Gly His Arg Phe Trp Val Gln Asp Thr Gly

145 150 155 160

Gly Trp Asp Pro Asn Val Lys Gly Ile His Ala Ser Ile Ala Gln Gln

165 170 175

Ala Glu Val Ala Met Ser Thr Ala Asp Val Ile Val Phe Val Val Asp

180 185 190

Thr Lys Val Gly Ile Thr Glu Thr Asp Ser Val Met Ala Ala Lys Leu

195 200 205

Leu Arg Ser Glu Val Pro Val Ile Leu Val Ala Asn Lys Phe Asp Ser

210 215 220

Asp Ser Gln Trp Ala Asp Met Ala Glu Phe Tyr Ser Leu Gly Leu Gly

225 230 235 240

Asp Pro Tyr Pro Val Ser Ala Gln His Gly Arg Gly Gly Ala Asp Val

245 250 255

Leu Asp Lys Val Leu Glu Leu Phe Pro Glu Glu Pro Arg Ser Lys Ser

260 265 270

Ile Val Glu Gly Pro Arg Arg Val Ala Leu Val Gly Lys Pro Asn Val

275 280 285

Gly Lys Ser Ser Leu Leu Asn Lys Phe Ala Gly Glu Thr Arg Ser Val

290 295 300

Val Asp Asn Val Ala Gly Thr Thr Val Asp Pro Val Asp Ser Leu Ile

305 310 315 320

Gln Leu Asp Gln Lys Leu Trp Lys Phe Val Asp Thr Ala Gly Leu Arg

325 330 335

Lys Lys Val Lys Thr Ala Ser Gly His Glu Tyr Tyr Ala Ser Leu Arg

340 345 350

Thr His Gly Ala Ile Asp Ala Ala Glu Leu Cys Val Leu Leu Ile Asp

355 360 365

Ser Ser Glu Pro Ile Thr Glu Gln Asp Gln Arg Val Leu Ala Met Ile

370 375 380

Thr Asp Ala Gly Lys Ala Leu Val Ile Ala Phe Asn Lys Trp Asp Leu

385 390 395 400

Met Asp Glu Asp Arg Arg Ile Asp Leu Asp Arg Glu Leu Asp Leu Gln

405 410 415

Leu Ala His Val Pro Trp Ala Lys Arg Ile Asn Ile Ser Ala Lys Thr

420 425 430

Gly Arg Ala Leu Gln Arg Leu Glu Pro Ala Met Leu Glu Ala Leu Asp

435 440 445

Asn Trp Asp Arg Arg Ile Ser Thr Gly Gln Leu Asn Thr Trp Leu Arg

450 455 460

Glu Ala Ile Ala Ala Asn Pro Pro Pro Met Arg Gly Gly Arg Leu Pro

465 470 475 480

Arg Val Leu Phe Ala Thr Gln Ala Ser Thr Gln Pro Pro Val Ile Val

485 490 495

Leu Phe Thr Thr Gly Phe Leu Glu Ala Gly Tyr Arg Arg Tyr Leu Glu

500 505 510

Arg Lys Phe Arg Glu Arg Phe Gly Phe Glu Gly Thr Pro Val Arg Ile

515 520 525

Ala Val Arg Val Arg Glu Arg Arg Gly Lys Gly Gly Asn Lys Gln

530 535 540

<210>19

<211>1261

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1231)

<223>RXA00813

<400>19

tccagcggct ggctaagtcc gtggagatgc atgggctgac cggttctttg ccgagggttt 60

taagctcagc atgcgacgcg gtcctcgggg aggtggcggc atg act gac att gat 115

Met Thr Asp Ile Asp

1 5

ctg gtg gtg gaa aac gtc caa agg att atc gcc acc aaa gag aca ccg 163

Leu Val Val Glu Asn Val Gln Arg Ile Ile Ala Thr Lys Glu Thr Pro

10 15 20

ccg acc tct gcg gaa ata gcg agc ctg att cgg gaa caa gca ggc gtg 211

Pro Thr Ser Ala Glu Ile Ala Ser Leu Ile Arg Glu Gln Ala Gly Val

25 30 35

atc agt aac gag gac atc gtg atg gtg ttg cgt cga ctg cgc agt gat 259

Ile Ser Asn Glu Asp Ile Val Met Val Leu Arg Arg Leu Arg Ser Asp

40 45 50

tct gtg ggc gtg gga ccg ttg gaa tct ctg ctt gcg ctt cct ggc gtg 307

Ser Val Gly Val Gly Pro Leu Glu Ser Leu Leu Ala Leu Pro Gly Val

55 60 65

acg gat gtg ttg gtt aat gcc cat gac agc gtg tgg att gat cgc ggt 355

Thr Asp Val Leu Val Asn Ala His Asp Ser Val Trp Ile Asp Arg Gly

70 75 80 85

cag ggc gtg gag aaa gtc gac atg gat ctg ggc tca gag gag gcg gtg 403

Gln Gly Val Glu Lys Val Asp Met Asp Leu Gly Ser Glu Glu Ala Val

90 95 100

cgt cgc ctt gcc acc cgg ttg gcg ttg acc tgt ggc aga cgc tta gat 451

Arg Arg Leu Ala Thr Arg Leu Ala Leu Thr Cys Gly Arg Arg Leu Asp

105 110 115

gat gcg cag cct ttc gct gat ggc cga atc acc agg gac gac ggc agc 499

Asp Ala Gln Pro Phe Ala Asp Gly Arg Ile Thr Arg Asp Asp Gly Ser

120 125 130

gtg ttg cgc att cac gcg gtg ttg gca ccc ttg gcg gaa tcc ggc acg 547

Val Leu Arg Ile His Ala Val Leu Ala Pro Leu Ala Glu Ser Gly Thr

135 140 145

tgc atc agt gtg cga gta ctg cgt caa gca cgg ctg agc ctt gat gat 595

Cys Ile Ser Val Arg Val Leu Arg Gln Ala Arg Leu Ser Leu Asp Asp

150 155 160 165

ctt atc caa agc ggc acg gtg cct gag gac atc gcg cct gcg ctc cgg 643

Leu Ile Gln Ser Gly Thr Val Pro Glu Asp Ile Ala Pro Ala Leu Arg

170 175 180

aac atc atc aat caa cgg cgc tcg ttc ctt gtt gtc ggt ggc acc ggc 691

Asn Ile Ile Asn Gln Arg Arg Ser Phe Leu Val Val Gly Gly Thr Gly

185 190 195

aca ggg aaa acc aca ttg ctg tcc gcg atg ctc acc gaa gtt ccc gct 739

Thr Gly Lys Thr Thr Leu Leu Ser Ala Met Leu Thr Glu Val Pro Ala

200 205 210

gat caa cga atc atc tgc atc gag gac acc gca gag ctt cat ccc ggc 787

Asp Gln Arg Ile Ile Cys Ile Glu Asp Thr Ala Glu Leu His Pro Gly

215 220 225

cat cca agc acc atc aac ttg gtg tct cgc caa gca aac gtc gag ggc 835

His Pro Ser Thr Ile Asn Leu Val Ser Arg Gln Ala Asn Val Glu Gly

230 235 240 245

gcc ggc gcc gtg agc atg gcg gat ttg ttg aaa caa tcg ctg cgc atg 883

Ala Gly Ala Val Ser Met Ala Asp Leu Leu Lys Gln Ser Leu Arg Met

250 255 260

agg cct gac cgg att gtc gtc gga gag att cgc ggt gcg gaa gtc gtg 931

Arg Pro Asp Arg Ile Val Val Gly Glu Ile Arg Gly Ala Glu Val Val

265 270 275

gat ctt ttg gct gcg atg aat acc gga cac gac ggc ggt gct ggc acc 979

Asp Leu Leu Ala Ala Met Asn Thr Gly His Asp Gly Gly Ala Gly Thr

280 285 290

att cac gcg aac tcc atc tct gaa gtt ccc gcg cgc atg gaa gct ctt 1027

Ile His Ala Asn Ser Ile Ser Glu Val Pro Ala Arg Met Glu Ala Leu

295 300 305

gcg gcg acc ggc gga ttg gac cgc atg gca ttg cat tct caa ctc gcg 1075

Ala Ala Thr Gly Gly Leu Asp Arg Met Ala Leu His Ser Gln Leu Ala

310 315 320 325

gcc gca gtg gac att gtg ctg gtc atg aaa cac acc cct ttt ggc cgc 1123

Ala Ala Val Asp Ile Val Leu Val Met Lys His Thr Pro Phe Gly Arg

330 335 340

agg cta gct caa ctc ggg gtg ctc cgc gga aat cct gtg acc acg cag 1171

Arg Leu Ala Gln Leu Gly Val Leu Arg Gly Asn Pro Val Thr Thr Gln

345 350 355

gtg gtg tgg gat ttg gac cac ggc atg cac gaa ggg agc gaa gag gca 1219

Val Val Trp Asp Leu Asp His Gly Met His Glu Gly Ser Glu Glu Ala

360 365 370

tgg ttt atg ccc taggccttct tagcgtggcg gtgttgatct 1261

Trp Phe Met Pro

375

<210>20

<211>377

<212>PRT

<213>谷氨酸棒状杆菌

<400>20

Met Thr Asp Ile Asp Leu Val Val Glu Asn Val Gln Arg Ile Ile Ala

1 5 10 15

Thr Lys Glu Thr Pro Pro Thr Ser Ala Glu Ile Ala Ser Leu Ile Arg

20 25 30

Glu Gln Ala Gly Val Ile Ser Asn Glu Asp Ile Val Met Val Leu Arg

35 40 45

Arg Leu Arg Ser Asp Ser Val Gly Val Gly Pro Leu Glu Ser Leu Leu

50 55 60

Ala Leu Pro Gly Val Thr Asp Val Leu Val Asn Ala His Asp Ser Val

65 70 75 80

Trp Ile Asp Arg Gly Gln Gly Val Glu Lys Val Asp Met Asp Leu Gly

85 90 95

Ser Glu Glu Ala Val Arg Arg Leu Ala Thr Arg Leu Ala Leu Thr Cys

100 105 110

Gly Arg Arg Leu Asp Asp Ala Gln Pro Phe Ala Asp Gly Arg Ile Thr

115 120 125

Arg Asp Asp Gly Ser Val Leu Arg Ile His Ala Val Leu Ala Pro Leu

130 135 140

Ala Glu Ser Gly Thr Cys Ile Ser Val Arg Val Leu Arg Gln Ala Arg

145 150 155 160

Leu Ser Leu Asp Asp Leu Ile Gln Ser Gly Thr Val Pro Glu Asp Ile

165 170 175

Ala Pro Ala Leu Arg Asn Ile Ile Asn Gln Arg Arg Ser Phe Leu Val

180 185 190

Val Gly Gly Thr Gly Thr Gly Lys Thr Thr Leu Leu Ser Ala Met Leu

195 200 205

Thr Glu Val Pro Ala Asp Gln Arg Ile Ile Cys Ile Glu Asp Thr Ala

210 215 220

Glu Leu His Pro Gly His Pro Ser Thr Ile Asn Leu Val Ser Arg Gln

225 230 235 240

Ala Asn Val Glu Gly Ala Gly Ala Val Ser Met Ala Asp Leu Leu Lys

245 250 255

Gln Ser Leu Arg Met Arg Pro Asp Arg Ile Val Val Gly Glu Ile Arg

260 265 270

Gly Ala Glu Val Val Asp Leu Leu Ala Ala Met Asn Thr Gly His Asp

275 280 285

Gly Gly Ala Gly Thr Ile His Ala Asn Ser Ile Ser Glu Val Pro Ala

290 295 300

Arg Met Glu Ala Leu Ala Ala Thr Gly Gly Leu Asp Arg Met Ala Leu

305 310 315 320

His Ser Gln Leu Ala Ala Ala Val Asp Ile Val Leu Val Met Lys His

325 330 335

Thr Pro Phe Gly Arg Arg Leu Ala Gln Leu Gly Val Leu Arg Gly Asn

340 345 350

Pro Val Thr Thr Gln Val Val Trp Asp Leu Asp His Gly Met His Glu

355 360 365

Gly Ser Glu Glu Ala Trp Phe Met Pro

370 375

<210>21

<211>880

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(850)

<223>RXA01081

<400>21

cattggtatt tgctcgaaag cacaacttaa aacagtaata atggtaatca cattcttttc 60

tattaagtaa gcaagtacac cggcccatta aagaggcacc atg acc cca gca aac 115

Met Thr Pro Ala Asn

1 5

gaa agt cct atg act aat cca tta ggt tct gcc ccc acc cca gcc aag 163

Glu Ser Pro Met Thr Asn Pro Leu Gly Ser Ala Pro Thr Pro Ala Lys

10 15 20

cca ctt ctt gac agt gtt ctt gat gag ctc ggt caa gat atc atc agt 211

Pro Leu Leu Asp Ser Val Leu Asp Glu Leu Gly Gln Asp Ile Ile Ser

25 30 35

ggc aag gtt gct gtc gga gat acc ttc aag ctg atg gac atc ggc gag 259

Gly Lys Val Ala Val Gly Asp Thr Phe Lys Leu Met Asp Ile Gly Glu

40 45 50

cgt ttt ggc att tcc cgc aca gtg gca cgc gaa gcg atg cgc gct ttg 307

Arg Phe Gly Ile Ser Arg Thr Val Ala Arg Glu Ala Met Arg Ala Leu

55 60 65

gag cag ctc ggt ctt gtc gct tct tca cgt cgc att ggc att act gtt 355

Glu Gln Leu Gly Leu Val Ala Ser Ser Arg Arg Ile Gly Ile Thr Val

70 75 80 85

ttg cca cag gaa gag tgg gct gtt ttt gat aag tcc atc att cgc tgg 403

Leu Pro Gln Glu Glu Trp Ala Val Phe Asp Lys Ser Ile Ile Arg Trp

90 95 100

cgt ctc aat gac gaa ggt cag cgt gaa ggc cag ctt cag tct ctt acc 451

Arg Leu Asn Asp Glu Gly Gln Arg Glu Gly Gln Leu Gln Ser Leu Thr

105 110 115

gag ctt cgt att gct att gaa ccg att gcc gcg cgc agc gtt gct ctt 499

Glu Leu Arg Ile Ala Ile Glu Pro Ile Ala Ala Arg Ser Val Ala Leu

120 125 130

cac gcg tca acc gcc gag ctc gag aaa atc cgc gcg ctc gca aca gag 547

His Ala Ser Thr Ala Glu Leu Glu Lys Ile Arg Ala Leu Ala Thr Glu

135 140 145

atg cgt cag ttg ggt gaa tct ggt cag ggt gcg tcc cag cgc ttc ctc 595

Met Arg Gln Leu Gly Glu Ser Gly Gln Gly Ala Ser Gln Arg Phe Leu

150 155 160 165

gaa gcg gac gtc act ttc cac gag ctc atc ttg cgt tat tgc cac aat 643

Glu Ala Asp Val Thr Phe His Glu Leu Ile Leu Arg Tyr Cys His Asn

170 175 180

gag atg ttc gct gca ctg att ccg tcg att agc gcg gtt ctt gtc ggc 691

Glu Met Phe Ala Ala Leu Ile Pro Ser Ile Ser Ala Val Leu Val Gly

185 190 195

cgc acc gag ctc ggc ctg cag cct gat ctg ccg gcg cac gag gcg cta 739

Arg Thr Glu Leu Gly Leu Gln Pro Asp Leu Pro Ala His Glu Ala Leu

200 205 210

gac aac cac gat aag ctt gcc gac gcc ctc ctt aac cgc gac gcc gac 787

Asp Asn His Asp Lys Leu Ala Asp Ala Leu Leu Asn Arg Asp Ala Asp

215 220 225

gcc gca gaa act gcg tcc cga aac atc ctc aat gag gtg cgc agc gcg 835

Ala Ala Glu Thr Ala Ser Arg Asn Ile Leu Asn Glu Val Arg Ser Ala

230 235 240 245

ctg ggc acg ctg aac taacgtgata cgcgcactgc gtttgccact 880

Leu Gly Thr Leu Asn

250

<210>22

<211>250

<212>PRT

<213>谷氨酸棒状杆菌

<400>22

Met Thr Pro Ala Asn Glu Ser Pro Met Thr Asn Pro Leu Gly Ser Ala

1 5 10 15

Pro Thr Pro Ala Lys Pro Leu Leu Asp Ser Val Leu Asp Glu Leu Gly

20 25 30

Gln Asp Ile Ile Ser Gly Lys Val Ala Val Gly Asp Thr Phe Lys Leu

35 40 45

Met Asp Ile Gly Glu Arg Phe Gly Ile Ser Arg Thr Val Ala Arg Glu

50 55 60

Ala Met Arg Ala Leu Glu Gln Leu Gly Leu Val Ala Ser Ser Arg Arg

65 70 75 80

Ile Gly Ile Thr Val Leu Pro Gln Glu Glu Trp Ala Val Phe Asp Lys

85 90 95

Ser Ile Ile Arg Trp Arg Leu Asn Asp Glu Gly Gln Arg Glu Gly Gln

100 105 110

Leu Gln Ser Leu Thr Glu Leu Arg Ile Ala Ile Glu Pro Ile Ala Ala

115 120 125

Arg Ser Val Ala Leu His Ala Ser Thr Ala Glu Leu Glu Lys Ile Arg

130 135 140

Ala Leu Ala Thr Glu Met Arg Gln Leu Gly Glu Ser Gly Gln Gly Ala

145 150 155 160

Ser Gln Arg Phe Leu Glu Ala Asp Val Thr Phe His Glu Leu Ile Leu

165 170 175

Arg Tyr Cys His Asn Glu Met Phe Ala Ala Leu Ile Pro Ser Ile Ser

180 185 190

Ala Val Leu Val Gly Arg Thr Glu Leu Gly Leu Gln Pro Asp Leu Pro

195 200 205

Ala His Glu Ala Leu Asp Asn His Asp Lys Leu Ala Asp Ala Leu Leu

210 215 220

Asn Arg Asp Ala Asp Ala Ala Glu Thr Ala Ser Arg Asn Ile Leu Asn

225 230 235 240

Glu Val Arg Ser Ala Leu Gly Thr Leu Asn

245 250

<210>23

<211>781

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(751)

<223>RXA01315

<400>23

catgtgcggt tgtgtcctaa gtcattgatg tcacctaacg cccctaagtt cactcggtgc 60

aatattgcac tgagtgcaag tttacactag gtttacttca gtg gat att gaa gag 115

Val Asp Ile Glu Glu

1 5

cag ccc tcg tta aga gaa atc aag cgc caa atg acc ctg gaa gcg ata 163

Gln Pro Ser Leu Arg Glu Ile Lys Arg Gln Met Thr Leu Glu Ala Ile

10 15 20

gaa gat aac gca acc agg ctc att ctg gag cgt ggc ttc gac aat gtc 211

Glu Asp Asn Ala Thr Arg Leu Ile Leu Glu Arg Gly Phe Asp Asn Val

25 30 35

aca atc gaa gac atc tgc gca gag gca ggg ata tcc aag cgc aca ttc 259

Thr Ile Glu Asp Ile Cys Ala Glu Ala Gly Ile Ser Lys Arg Thr Phe

40 45 50

ttt aac tac gtg gag tcc aaa gag tct gtg gcc atc ggg cac aca gcc 307

Phe Asn Tyr Val Glu Ser Lys Glu Ser Val Ala Ile Gly His Thr Ala

55 60 65

aag ctc cca acg gat gaa gaa cgt gaa gca ttc ctg gct acg cgt cat 355

Lys Leu Pro Thr Asp Glu Glu Arg Glu Ala Phe Leu Ala Thr Arg His

70 75 80 85

gaa aat att atc gat act gta ttt gac ctg gta atc aac ctc ttt ggc 403

Glu Asn Ile Ile Asp Thr Val Phe Asp Leu Val Ile Asn Leu Phe Gly

90 95 100

aac cac gac aac tcc aag tct gga gtt gca ggc gac att atg cgt cga 451

Asn His Asp Asn Ser Lys Ser Gly Val Ala Gly Asp Ile Met Arg Arg

105 110 115

cgc aaa gag atc cgg gtg aag cat cca gaa ctg gca gtg caa cat ttc 499

Arg Lys Glu Ile Arg Val Lys His Pro Glu Leu Ala Val Gln His Phe

120 125 130

gcc agg ttc cac caa gca cgc gaa ggg cta gaa cac cta att gtt gag 547

Ala Arg Phe His Gln Ala Arg Glu Gly Leu Glu His Leu I1e Val Glu

135 140 145

tac ttc gaa aaa tgg cca ggc tcc caa cat cta gat gag cct gca gat 595

Tyr Phe Glu Lys Trp Pro Gly Ser Gln His Leu Asp Glu Pro Ala Asp

150 155 160 165

cga gaa gca atc gcc ata gtt ggc ctg ctg atc tcg gtc atg ctt caa 643

Arg Glu Ala I1e Ala Ile Val Gly Leu Leu Ile Ser Val Met Leu Gln

170 175 180

ggt tct cgt gaa tgg cac gac atg cca caa ggc acg caa gct gat ttc 691

Gly Ser Arg Glu Trp His Asp Met Pro Gln Gly Thr Gln Ala Asp Phe

185 190 195

caa gcc tgc tgt cgc aaa gca att aaa aat act ttt ctt ctt aga ggt 739

Gln Ala Cys Cys Arg Lys Ala Ile Lys Asn Thr Phe Leu Leu Arg Gly

200 205 210

gga ttt tca gaa tgacatcaca ggtcaagccg gacgacgaac 781

Gly Phe Ser Glu

215

<210>24

<211>217

<212>PRT

<213>谷氨酸棒状杆菌

<400>24

Val Asp Ile Glu Glu Gln Pro Ser Leu Arg Glu Ile Lys Arg Gln Met

1 5 10 15

Thr Leu Glu Ala Ile Glu Asp Asn Ala Thr Arg Leu Ile Leu Glu Arg

20 25 30

Gly Phe Asp Asn Val Thr Ile Glu Asp Ile Cys Ala Glu Ala Gly Ile

35 40 45

Ser Lys Arg Thr Phe Phe Asn Tyr Val Glu Ser Lys Glu Ser Val Ala

50 55 60

Ile Gly His Thr Ala Lys Leu Pro Thr Asp Glu Glu Arg Glu Ala Phe

65 70 75 80

Leu Ala Thr Arg His Glu Asn Ile Ile Asp Thr Val Phe Asp Leu Val

85 90 95

Ile Asn Leu Phe Gly Asn His Asp Asn Ser Lys Ser Gly Val Ala Gly

100 105 110

Asp I1e Met Arg Arg Arg Lys Glu Ile Arg Val Lys His Pro Glu Leu

115 120 125

Ala Val Gln His Phe Ala Arg Phe His Gln Ala Arg Glu Gly Leu Glu

130 135 140

His Leu Ile Val Glu Tyr Phe Glu Lys Trp Pro Gly Ser Gln His Leu

145 150 155 160

Asp Glu Pro Ala Asp Arg Glu Ala Ile Ala Ile Val Gly Leu Leu Ile

165 170 175

Ser Val Met Leu Gln Gly Ser Arg Glu Trp His Asp Met Pro Gln Gly

180 185 190

Thr Gln Ala Asp Phe Gln Ala Cys Cys Arg Lys Ala Ile Lys Asn Thr

195 200 205

Phe Leu Leu Arg Gly Gly Phe Ser Glu

210 215

<210>25

<211>2212

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2182)

<223>RXA01573

<400>25

agccccagct caccgaattc tccattcgtt ttaattgctt cgttaattaa aacgccatat 60

aaaaaccggc gcattgccgg tatttttcca ggagaattta atg aag agg ctt tcc 115

Met Lys Arg Leu Ser

l 5

cgt gca gcc ctc gca gtg gtc gcc acc acc gca gtt agc ttc agc gca 163

Arg Ala Ala Leu Ala Val Val Ala Thr Thr Ala Val Ser Phe Ser Ala

10 15 20

ctc gca gtt cca gct ttc gca gac gaa gca agc aat gtt gag ctc aac 211

Leu Ala Val Pro Ala Phe Ala Asp Glu Ala Ser Asn Val Glu Leu Asn

25 30 35

atc ctc ggt gtc acc gac ttc cac gga cac atc gag cag aag gct gtt 259

Ile Leu Gly Val Thr Asp Phe His Gly His Ile Glu Gln Lys Ala Val

40 45 50

aaa gat gat aag gga gta atc acc ggt tac tca gaa atg ggt gcc agt 307

Lys Asp Asp Lys Gly Val Ile Thr Gly Tyr Ser Glu Met Gly Ala Ser

55 60 65

ggc gtt gcc tgc tac gtc gac gct gaa cgc gcg gac aac cca aac acc 355

Gly Val Ala Cys Tyr Val Asp Ala Glu Arg Ala Asp Asn Pro Asn Thr

70 75 80 85

cgc ttc atc acc gtt ggt gac aac att ggt gga tcc cca ttc gtg tcc 403

Arg Phe Ile Thr Val Gly Asp Asn Ile Gly Gly Ser Pro Phe Val Ser

90 95 100

tcc atc ctg aag gat gag cca acc ttg caa gcc ctc agc gcc atc ggt 451

Ser Ile Leu Lys Asp Glu Pro Thr Leu Gln Ala Leu Ser Ala Ile Gly

105 110 115

gtt gac gca tcc gca ctg ggc aat cac gaa ttc gac cag ggc tac tca 499

Val Asp Ala Ser Ala Leu Gly Asn His Glu Phe Asp Gln Gly Tyr Ser

120 125 130

gac ctg gtg aac cgc gtt tcc ctc gac ggc tcc ggc agc gca aag ttc 547

Asp Leu Val Asn Arg Val Ser Leu Asp Gly Ser Gly Ser Ala Lys Phe

135 140 145

cca tac ctc ggc gca aac gtt gaa ggt ggc acc cca gca cct gca aag 595

Pro Tyr Leu Gly Ala Asn Val Glu Gly Gly Thr Pro Ala Pro Ala Lys

150 155 160 165

tct gaa atc atc gag atg gac ggc gtc aag atc gct tac gtc ggc gca 643

Ser Glu Ile Ile Glu Met Asp Gly Val Lys Ile Ala Tyr Val Gly Ala

170 175 180

gta acc gag gag acc gca acc ttg gtc tcc cca gca ggc atc gaa ggc 691

Val Thr Glu Glu Thr Ala Thr Leu Val Ser Pro Ala Gly Ile Glu Gly

185 190 195

atc acc ttc acc ggc gac atc gac gct atc aac gca gaa gca gat cgc 739

Ile Thr Phe Thr Gly Asp Ile Asp Ala Ile Asn Ala Glu Ala Asp Arg

200 205 210

gtc att gag gca ggc gaa gca gac gta gtc atc gca ttg atc cac gct 787

Val Ile Glu Ala Gly Glu Ala Asp Val Val Ile Ala Leu Ile His Ala

215 220 225

gaa gcc gct cca acc gat cta ttc tcc aac aac gtt gac gtt gta ttc 835

Glu Ala Ala Pro Thr Asp Leu Phe Ser Asn Asn Val Asp Val Val Phe

230 235 240 245

tcc gga cac acc cac ttc gac tac gtt gct gaa ggc gaa gca cgt ggc 883

Ser Gly His Thr His Phe Asp Tyr Val Ala Glu Gly Glu Ala Arg Gly

250 255 260

gac aag cag cca ctc gtt gtc atc cag ggc cac gaa tac ggc aag gtc 931

Asp Lys Gln Pro Leu Val Val Ile Gln Gly His Glu Tyr Gly Lys Val

265 270 275

atc tcc gac gtg gag atc tcc tac gac cgc gaa gca ggc aag atc acc 979

Ile Ser Asp Val Glu Ile Ser Tyr Asp Arg Glu Ala Gly Lys Ile Thr

280 285 290

aac att gag gcg aag aat gtc tct gct act gac gtt gtg gaa aac tgt 1027

Asn Ile Glu Ala Lys Asn Val Ser Ala Thr Asp Val Val Glu Asn Cys

295 300 305

gag act cca aac aca gca gtc gac gca atc gtt gca gct gct gtt gag 1075

Glu Thr Pro Asn Thr Ala Val Asp Ala Ile Val Ala Ala Ala Val Glu

310 315 320 325

gcc gct gaa gaa gca ggt aat gaa gtt gtt gca acc att gac aac ggc 1123

Ala Ala Glu Glu Ala Gly Asn Glu Val Val Ala Thr Ile Asp Asn Gly

330 335 340

ttc tac cgt ggg gcg gat gaa gag ggt acg acc ggc tcc aac cgt ggt 117l

Phe Tyr Arg Gly Ala Asp Glu Glu Gly Thr Thr Gly Ser Asn Arg Gly

345 350 355

gtt gag tct tcc ctg agc aac ctc atc gca gaa gct gga ctg tgg gca 1219

Val Glu Ser Ser Leu Ser Asn Leu Ile Ala Glu Ala Gly Leu Trp Ala

360 365 370

gtc aac gac gcg acc atc ctg aac gct gac atc ggc atc atg aac gca 1267

Val Asn Asp Ala Thr Ile Leu Asn Ala Asp Ile Gly Ile Met Asn Ala

375 380 385

ggc ggc gtg cgt gcg gac ctc gaa gca ggc gaa gtt acc ttc gca gat 1315

Gly Gly Val Arg Ala Asp Leu Glu Ala Gly Glu Val Thr Phe Ala Asp

390 395 400 405

gca tac gca acc cag aac ttc tcc aac acc tac ggc gta cgt gaa gtg 1363

Ala Tyr Ala Thr Gln Asn Phe Ser Asn Thr Tyr Gly Val Arg Glu Val

410 415 420

tct ggt gcg cag ttc aaa gaa gca ctg gaa cag cag tgg aag gaa acc 1411

Ser Gly Ala Gln Phe Lys Glu Ala Leu Glu Gln Gln Trp Lys Glu Thr

425 430 435

ggc gac cgc cca cgt ctg gca ttg gga ctg tcc agc aac gtc cag tac 1459

Gly Asp Arg Pro Arg Leu Ala Leu Gly Leu Ser Ser Asn Val Gln Tyr

440 445 450

tcc tac gac gag acc cgc gaa tac ggc gac cgc atc acc cac atc acc 1507

Ser Tyr Asp Glu Thr Arg Glu Tyr Gly Asp Arg Ile Thr His Ile Thr

455 460 465

ttc aac ggt gag cca atg gat atg aag gag acc tac cgc gtc aca gga 1555

Phe Asn Gly Glu Pro Met Asp Met Lys Glu Thr Tyr Arg Val Thr Gly

470 475 480 485

tca tcc ttc ctg ctc gca ggt ggc gac tcc ttc act gca ttc gct gaa 1603

Ser Ser Phe Leu Leu Ala Gly Gly Asp Ser Phe Thr Ala Phe Ala Glu

490 495 500

ggc ggc cca atc gct gaa acc ggc atg gtt gac att gac ctg ttc aac 1651

Gly Gly Pro Ile Ala Glu Thr Gly Met Val Asp Ile Asp Leu Phe Asn

505 510 515

aac tac atc gca gct cac cca gat gca cca att cgt gca aat cag agc 1699

Asn Tyr Ile Ala Ala His Pro Asp Ala Pro Ile Arg Ala Asn Gln Ser

520 525 530

tca gta ggc atc gcc ctt tcc ggc ccg gca gtt gca gaa gac gga act 1747

Ser Val Gly Ile Ala Leu Ser Gly Pro Ala Val Ala Glu Asp Gly Thr

535 540 545

ttg gtc cct ggt gaa gag ctg acc gtc gat ctt tct tcc ctc tcc tac 1795

Leu Val Pro Gly Glu Glu Leu Thr Val Asp Leu Ser Ser Leu Ser Tyr

550 555 560 565

acc gga cct gaa gct aag cca acc acc gtt gag gtg acc gtt ggt act 1843

Thr Gly Pro Glu Ala Lys Pro Thr Thr Val Glu Val Thr Val Gly Thr

570 575 580

gag aag aag act gcg gac gtc gat aac acc atc gtt cct cag ttt gac 1891

Glu Lys Lys Thr Ala Asp Val Asp Asn Thr Ile Val Pro Gln Phe Asp

585 590 595

agc acc ggc aag gca act gtc acc ctg act gtt cct gag gga gct acc 1939

Ser Thr Gly Lys Ala Thr Val Thr Leu Thr Val Pro Glu Gly Ala Thr

600 605 610

tct gtc aag atc gca act gac aat ggc act acc ttt gaa ctg cca gta 1987

Ser Val Lys Ile Ala Thr Asp Asn Gly Thr Thr Phe Glu Leu Pro Val

615 620 625

acc gta aac ggt gaa ggc aac aat gat gac gat gat gat aag gag cag 2035

Thr Val Asn Gly Glu Gly Asn Asn Asp Asp Asp Asp Asp Lys Glu Gln

630 635 640 645

cag tcc tcc gga tcc tcc gac gcc ggt tcc ctt gta gca gtt ctc ggt 2083

Gln Ser Ser Gly Ser Ser Asp Ala Gly Ser Leu Val Ala Val Leu Gly

650 655 660

gtt ctt gga gca ctc ggt ggc ctg gtg gcg ttc ttc ctg aac tct gcg 2131

Val Leu Gly Ala Leu Gly Gly Leu Val Ala Phe Phe Leu Asn Ser Ala

665 670 675

cag ggc gca cca ttc ttg gct cag ctt cag gct atg ttt gcg cag ttc 2179

Gln Gly Ala Pro Phe Leu Ala Gln Leu Gln Ala Met Phe Ala Gln Phe

680 685 690

atg taataacttg tagtaaataa atcgggcctt 2212

Met

<210>26

<211>694

<212>PRT

<213>谷氨酸棒状杆菌

<400>26

Met Lys Arg Leu Ser Arg Ala Ala Leu Ala Val Val Ala Thr Thr Ala

1 5 10 15

Val Ser Phe Ser Ala Leu Ala Val Pro Ala Phe Ala Asp Glu Ala Ser

20 25 30

Asn Val Glu Leu Asn Ile Leu Gly Val Thr Asp Phe His Gly His Ile

35 40 45

Glu Gln Lys Ala Val Lys Asp Asp Lys Gly Val Ile Thr Gly Tyr Ser

50 55 60

Glu Met Gly Ala Ser Gly Val Ala Cys Tyr Val Asp Ala Glu Arg Ala

65 70 75 80

Asp Asn Pro Asn Thr Arg Phe Ile Thr Val Gly Asp Asn Ile Gly Gly

85 90 95

Ser Pro Phe Val Ser Ser Ile Leu Lys Asp Glu Pro Thr Leu Gln Ala

100 105 110

Leu Ser Ala Ile Gly Val Asp Ala Ser Ala Leu Gly Asn His Glu Phe

115 120 125

Asp Gln Gly Tyr Ser Asp Leu Val Asn Arg Val Ser Leu Asp Gly Ser

130 135 140

Gly Ser Ala Lys Phe Pro Tyr Leu Gly Ala Asn Val Glu Gly Gly Thr

145 150 155 160

Pro Ala Pro Ala Lys Ser Glu Ile Ile Glu Met Asp Gly Val Lys Ile

165 170 175

Ala Tyr Val Gly Ala Val Thr Glu Glu Thr Ala Thr Leu Val Ser Pro

180 185 190

Ala Gly Ile Glu Gly Ile Thr Phe Thr Gly Asp Ile Asp Ala Ile Asn

195 200 205

Ala Glu Ala Asp Arg Val Ile Glu Ala Gly Glu Ala Asp Val Val Ile

210 215 220

Ala Leu Ile His Ala Glu Ala Ala Pro Thr Asp Leu Phe Ser Asn Asn

225 230 235 240

Val Asp Val Val Phe Ser Gly His Thr His Phe Asp Tyr Val Ala Glu

245 250 255

Gly Glu Ala Arg Gly Asp Lys Gln Pro Leu Val Val Ile Gln Gly His

260 265 270

Glu Tyr Gly Lys Val Ile Ser Asp Val Glu Ile Ser Tyr Asp Arg Glu

275 280 285

Ala Gly Lys Ile Thr Asn Ile Glu Ala Lys Asn Val Ser Ala Thr Asp

290 295 300

Val Val Glu Asn Cys Glu Thr Pro Asn Thr Ala Val Asp Ala Ile Val

305 310 315 320

Ala Ala Ala Val Glu Ala Ala Glu Glu Ala Gly Asn Glu Val Val Ala

325 330 335

Thr Ile Asp Asn Gly Phe Tyr Arg Gly Ala Asp Glu Glu Gly Thr Thr

340 345 350

Gly Ser Asn Arg Gly Val Glu Ser Ser Leu Ser Asn Leu Ile Ala Glu

355 360 365

Ala Gly Leu Trp Ala Val Asn Asp Ala Thr Ile Leu Asn Ala Asp Ile

370 375 380

Gly Ile Met Asn Ala Gly Gly Val Arg Ala Asp Leu Glu Ala Gly Glu

385 390 395 400

Val Thr Phe Ala Asp Ala Tyr Ala Thr Gln Asn Phe Ser Asn Thr Tyr

405 410 415

Gly Val Arg Glu Val Ser Gly Ala Gln Phe Lys Glu Ala Leu Glu Gln

420 425 430

Gln Trp Lys Glu Thr Gly Asp Arg Pro Arg Leu Ala Leu Gly Leu Ser

435 440 445

Ser Asn Val Gln Tyr Ser Tyr Asp Glu Thr Arg Glu Tyr Gly Asp Arg

450 455 460

Ile Thr His Ile Thr Phe Asn Gly Glu Pro Met Asp Met Lys Glu Thr

465 470 475 480

Tyr Arg Val Thr Gly Ser Ser Phe Leu Leu Ala Gly Gly Asp Ser Phe

485 490 495

Thr Ala Phe Ala Glu Gly Gly Pro Ile Ala Glu Thr Gly Met Val Asp

500 505 510

Ile Asp Leu Phe Asn Asn Tyr Ile Ala Ala His Pro Asp Ala Pro Ile

515 520 525

Arg Ala Asn Gln Ser Ser Val Gly Ile Ala Leu Ser Gly Pro Ala Val

530 535 540

Ala Glu Asp Gly Thr Leu Val Pro Gly Glu Glu Leu Thr Val Asp Leu

545 550 555 560

Ser Ser Leu Ser Tyr Thr Gly Pro Glu Ala Lys Pro Thr Thr Val Glu

565 570 575

Val Thr Val Gly Thr Glu Lys Lys Thr Ala Asp Val Asp Asn Thr Ile

580 585 590

Val Pro Gln Phe Asp Ser Thr Gly Lys Ala Thr Val Thr Leu Thr Val

595 600 605

Pro Glu Gly Ala Thr Ser Val Lys Ile Ala Thr Asp Asn Gly Thr Thr

610 615 620

Phe Glu Leu Pro Val Thr Val Asn Gly Glu Gly Asn Asn Asp Asp Asp

625 630 635 640

Asp Asp Lys Glu Gln Gln Ser Ser Gly Ser Ser Asp Ala Gly Ser Leu

645 650 655

Val Ala Val Leu Gly Val Leu Gly Ala Leu Gly Gly Leu Val Ala Phe

660 665 670

Phe Leu Asn Ser Ala Gln Gly Ala Pro Phe Leu Ala Gln Leu Gln Ala

675 680 685

Met Phe Ala Gln Phe Met

690

<210>27

<211>760

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(730)

<223>RXA01607

<400>27

gggctgaagg gctgggcgga acaataatta ttgaatctac aatcggatcg ggaactggaa 60

tttccgcccg ttttccctat ccacaaaagg accaagataa gtg atc cgt att ctg 115

Val Ile Arg Ile Leu

1 5

ttg gct gat gat cat ccc gtt gtt cgc gca ggc ctt gcc tcc ttg ctg 163

Leu Ala Asp Asp His Pro Val Val Arg Ala Gly Leu Ala Ser Leu Leu

10 15 20

gtg agt gaa gat gat ttt gag ata gtg gac atg gtg ggc acc cca gat 211

Val Ser Glu Asp Asp Phe Glu Ile Val Asp Met Val Gly Thr Pro Asp

25 30 35

gat gcc gtt gcg cgc gcc gcg gaa ggc ggg gtg gat gtg gtg ttg atg 259

Asp Ala Val Ala Arg Ala Ala Glu Gly Gly Val Asp Val Val Leu Met

40 45 50

gat ctg cgt ttt ggt gat caa cca ggc atc gag gtc gcc ggc ggg gta 307

Asp Leu Arg Phe Gly Asp Gln Pro Gly Ile Glu Val Ala Gly Gly Val

55 60 65

gag gca acg cgt cgc atc cgt gcg ctg gac aac ccg cca cag gta ctg 355

Glu Ala Thr Arg Arg Ile Arg Ala Leu Asp Asn Pro Pro Gln Val Leu

70 75 80 85

gtg gtg acc aac tac tcc aca gac ggc gat gtg gtg ggc gca gta tct 403

Val Val Thr Asn Tyr Ser Thr Asp Gly Asp Val Val Gly Ala Val Ser

90 95 100

gct ggt gcc gtg ggg tat ttg ctc aaa gat agc tcc cca gaa gat ctc 451

Ala Gly Ala Val Gly Tyr Leu Leu Lys Asp Ser Ser Pro Glu Asp Leu

105 110 115

att gcc ggt gtt cgc gat gcc gcg cgg gga gaa tca gtg ctt tca aag 499

Ile Ala Gly Val Arg Asp Ala Ala Arg Gly Glu Ser Val Leu Ser Lys

120 125 130

cag gtc gcc agc aag atc atg ggg cgg atg aac aac ccc atg act gct 547

Gln Val Ala Ser Lys Ile Met Gly Arg Met Asn Asn Pro Met Thr Ala

135 140 145

ctc agt gcc aga gaa att gaa gtg ctg tcc ttg gtg gcg caa ggg caa 595

Leu Ser Ala Arg Glu Ile Glu Val Leu Ser Leu Val Ala Gln Gly Gln

150 155 160 165

agc aat aga gaa atc ggc aag aaa ctt ttc ctc act gag gcc acg gtg 643

Ser Asn Arg Glu Ile Gly Lys Lys Leu Phe Leu Thr Glu Ala Thr Val

170 175 180

aaa agt cac atg ggg cat gtg ttc aac aag ctg gat gtc acc tct aga 691

Lys Ser His Met Gly His Val Phe Asn Lys Leu Asp Val Thr Ser Arg

185 190 195

aca gct gcg gta gct gaa gcc aga cag cgc gga att atc tagacgcaca 740

Thr Ala Ala Val Ala Glu Ala Arg Gln Arg Gly Ile Ile

200 205 210

cgtgttggta accgatcaca 760

<210>28

<211>210

<212>PRT

<213>谷氨酸棒状杆菌

<400>28

Val Ile Arg Ile Leu Leu Ala Asp Asp His Pro Val Val Arg Ala Gly

1 5 10 15

Leu Ala Ser Leu Leu Val Ser Glu Asp Asp Phe Glu Ile Val Asp Met

20 25 30

Val Gly Thr Pro Asp Asp Ala Val Ala Arg Ala Ala Glu Gly Gly Val

35 40 45

Asp Val Val Leu Met Asp Leu Arg Phe Gly Asp Gln Pro Gly Ile Glu

50 55 60

Val Ala Gly Gly Val Glu Ala Thr Arg Arg Ile Arg Ala Leu Asp Asn

65 70 75 80

Pro Pro Gln Val Leu Val Val Thr Asn Tyr Ser Thr Asp Gly Asp Val

85 90 95

Val Gly Ala Val Ser Ala Gly Ala Val Gly Tyr Leu Leu Lys Asp Ser

100 105 110

Ser Pro Glu Asp Leu Ile Ala Gly Val Arg Asp Ala Ala Arg Gly Glu

115 120 125

Ser Val Leu Ser Lys Gln Val Ala Ser Lys Ile Met Gly Arg Met Asn

130 135 140

Asn Pro Met Thr Ala Leu Ser Ala Arg Glu Ile Glu Val Leu Ser Leu

145 150 155 160

Val Ala Gln Gly Gln Ser Asn Arg Glu Ile Gly Lys Lys Leu Phe Leu

165 170 175

Thr Glu Ala Thr Val Lys Ser His Met Gly His Val Phe Asn Lys Leu

180 185 190

Asp Val Thr Ser Arg Thr Ala Ala Val Ala Glu Ala Arg Gln Arg Gly

195 200 205

Ile Ile

210

<210>29

<211>892

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(862)

<223>RXA01759

<400>29

cactaaagaa cttctgagcg cgctatcgtt ggtcgatgct attggtctgg gtacttctcc 60

ggtagaccat cactctgaat aagggggata acatatagtt atg acc aaa cgg ctc 115

Met Thr Lys Arg Leu

l 5

agc ctt gaa ggg ctc cgc tat gcg cag gcc gtc gca gaa act cac tca 163

Ser Leu Glu Gly Leu Arg Tyr Ala Gln Ala Val Ala Glu Thr His Ser

10 15 20

ttc agc gca gca gcc cgc gaa tac gga gtc acc caa cct gcg cta tcc 211

Phe Ser Ala Ala Ala Arg Glu Tyr Gly Val Thr Gln Pro Ala Leu Ser

25 30 35

aac ggc atc gcc aaa ctg gaa gat cgg ctc ggt gaa caa ctc ttc gat 259

Asn Gly Ile Ala Lys Leu Glu Asp Arg Leu Gly Glu Gln Leu Phe Asp

40 45 50

cga tct act caa ggc gtc acc ccg acg tcc ttt ggc ctc cac atc ctc 307

Arg Ser Thr Gln Gly Val Thr Pro Thr Ser Phe Gly Leu His Ile Leu

55 60 65

ccc ctg atc caa cgc gcg ctg act gaa atc gac gca atc acc gcg gaa 355

Pro Leu Ile Gln Arg Ala Leu Thr Glu Ile Asp Ala Ile Thr Ala Glu

70 75 80 85

gcg cac cgt ttg att aac tca gaa gca cgc agc att cga gtt gga atc 403

Ala His Arg Leu Ile Asn Ser Glu Ala Arg Ser Ile Arg Val Gly Ile

90 95 100

tcc cca ctt atc aac cct caa ctg gtt gca cga aca tat acc gcg gtt 451

Ser Pro Leu Ile Asn Pro Gln Leu Val Ala Arg Thr Tyr Thr Ala Val

105 110 115

cgt gag ctt ccc aca gca cac gac cta gta ctc cgc gaa gca aac atg 499

Arg Glu Leu Pro Thr Ala His Asp Leu Val Leu Arg Glu Ala Asn Met

120 125 130

aaa gaa cta cat gaa gga ctt ctt gca ggt gaa ctt aat gta att ctc 547

Lys Glu Leu His Glu Gly Leu Leu Ala Gly Glu Leu Asn Val Ile Leu

135 140 145

att ccc gca gtg aaa cca cta ccc cat ttt gaa cac cgc atc att gac 595

Ile Pro Ala Val Lys Pro Leu Pro His Phe Glu His Arg Ile Ile Asp

150 155 160 165

tcc gaa cca gtc gtt atc gtc gaa tcc acc cag gac agc acc gac ccc 643

Ser Glu Pro Val Val Ile Val Glu Ser Thr Gln Asp Ser Thr Asp Pro

170 175 180

ata gaa ctt cgc gag act cag cac gaa ccg ttc att ctg gta ccc gac 691

Ile Glu Leu Arg Glu Thr Gln His Glu Pro Phe Ile Leu Val Pro Asp

185 190 195

aca tgc ggt tta acc act ttc acc aat caa ctg ttt gaa aca aat gac 739

Thr Cys Gly Leu Thr Thr Phe Thr Asn Gln Leu Phe Glu Thr Asn Asp

200 205 210

ctg gca tta aac gcc tat tcc ggc gaa gca gcc agc tac caa gta ctc 787

Leu Ala Leu Asn Ala Tyr Ser Gly Glu Ala Ala Ser Tyr Gln Val Leu

215 220 225

gaa cag tgg gcc aca ctt gga ctc gga tct gca atg ctt cca ctt tct 835

Glu Gln Trp Ala Thr Leu Gly Leu Gly Ser Ala Met Leu Pro Leu Ser

230 235 240 245

aaa ctc agc tcc cct aca gca ccc cat tgaccactcc gcgaacaagg 882

Lys Leu Ser Ser Pro Thr Ala Pro His

250

cctcgacgtg 892

<210>30

<211>254

<212>PRT

<213>谷氨酸棒状杆菌

<400>30

Met Thr Lys Arg Leu Ser Leu Glu Gly Leu Arg Tyr Ala Gln Ala Val

1 5 10 15

Ala Glu Thr His Ser Phe Ser Ala Ala Ala Arg Glu Tyr Gly Val Thr

20 25 30

Gln Pro Ala Leu Ser Asn Gly Ile Ala Lys Leu Glu Asp Arg Leu Gly

35 40 45

Glu Gln Leu Phe Asp Arg Ser Thr Gln Gly Val Thr Pro Thr Ser Phe

50 55 60

Gly Leu His Ile Leu Pro Leu Ile Gln Arg Ala Leu Thr Glu Ile Asp

65 70 75 80

Ala Ile Thr Ala Glu Ala His Arg Leu Ile Asn Ser Glu Ala Arg Ser

85 90 95

Ile Arg Val Gly Ile Ser Pro Leu Ile Asn Pro Gln Leu Val Ala Arg

100 105 110

Thr Tyr Thr Ala Val Arg Glu Leu Pro Thr Ala His Asp Leu Val Leu

115 120 125

Arg Glu Ala Asn Met Lys Glu Leu His Glu Gly Leu Leu Ala Gly Glu

130 135 140

Leu Asn Val Ile Leu Ile Pro Ala Val Lys Pro Leu Pro His Phe Glu

145 150 155 160

His Arg Ile Ile Asp Ser Glu Pro Val Val Ile Val Glu Ser Thr Gln

165 170 175

Asp Ser Thr Asp Pro Ile Glu Leu Arg Glu Thr Gln His Glu Pro Phe

180 185 190

Ile Leu Val Pro Asp Thr Cys Gly Leu Thr Thr Phe Thr Asn Gln Leu

195 200 205

Phe Glu Thr Asn Asp Leu Ala Leu Asn Ala Tyr Ser Gly Glu Ala Ala

210 215 220

Ser Tyr Gln Val Leu Glu Gln Trp Ala Thr Leu Gly Leu Gly Ser Ala

225 230 235 240

Met Leu Pro Leu Ser Lys Leu Ser Ser Pro Thr Ala Pro His

245 250

<210>31

<211>2068

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2038)

<223>RXA01826

<400>31

ctacacctag gacgagtgct agcgttccag ttgagactaa tgcaccggct gatgatttaa 60

tcgacgccgt aaatggccta ttggatgtag gaggagcgca gtg acc ttc gtg atc 115

Val Thr Phe Val Ile

1 5

gct gat cgc tat gaa ctg gat gcc gtc atc ggc tcc ggt ggc atg agc 163

Ala Asp Arg Tyr Glu Leu Asp Ala Val Ile Gly Ser Gly Gly Met Ser

10 15 20

gag gtg ttc gcg gcc acc gac acg ctc att ggt cgg gag gtc gcg gta 211

Glu Val Phe Ala Ala Thr Asp Thr Leu Ile Gly Arg Glu Val Ala Val

25 30 35

aag atg ctg cgc atc gac ctt gcg aaa gat ccc aat ttc cga gaa cgc 259

Lys Met Leu Arg Ile Asp Leu Ala Lys Asp Pro Asn Phe Arg Glu Arg

40 45 50

ttc cgc agg gaa gcc caa aac tcc gga agg ttg agc cac tct tcg atc 307

Phe Arg Arg Glu Ala Gln Asn Ser Gly Arg Leu Ser His Ser Ser Ile

55 60 65

gtc gct gtt ttt gac acc ggc gaa gta gac aaa gac ggc acc tct gtt 355

Val Ala Val Phe Asp Thr Gly Glu Val Asp Lys Asp Gly Thr Ser Val

70 75 80 85

ccc tac att gtg atg gaa cgc gtg cag ggt cga aac ctg cgc gaa gtt 403

Pro Tyr Ile Val Met Glu Arg Val Gln Gly Arg Asn Leu Arg Glu Val

90 95 100

gtc acc gaa gac ggc gta ttc acc cca gtt gag gca gcc aac atc ctc 45l

Val Thr Glu Asp Gly Val Phe Thr Pro Val Glu Ala Ala Asn Ile Leu

105 110 115

atc cct gtg tgt gaa gcg ctg cag gca tcc cat gac gcc ggc att att 499

Ile Pro Val Cys Glu Ala Leu Gln Ala Ser His Asp Ala Gly Ile Ile

120 125 130

cac cgc gat gtg aaa ccc gcc aac atc atg atc acc aac acc ggt ggc 547

His Arg Asp Val Lys Pro Ala Asn Ile Met Ile Thr Asn Thr Gly Gly

135 140 145

gtg aaa gtc atg gac ttc ggc atc gcc cgc gcg gtc aac gat tcc acc 595

Val Lys Val Met Asp Phe Gly Ile Ala Arg Ala Val Asn Asp Ser Thr

150 155 160 165

tcc gcc atg act caa acc tcc gca gtc atc ggc acc gcc cag tac ctc 643

Ser Ala Met Thr Gln Thr Ser Ala Val Ile Gly Thr Ala Gln Tyr Leu

170 175 180

tcc cct gag cag gcc cgc ggc aaa ccc gcc gat gcg cgt tcc gat att 691

Ser Pro Glu Gln Ala Arg Gly Lys Pro Ala Asp Ala Arg Ser Asp Ile

185 190 195

tac gcc acc ggc tgc gtc atg tac gaa tta gtc acc ggt aag cca cct 739

Tyr Ala Thr Gly Cys Val Met Tyr Glu Leu Val Thr Gly Lys Pro Pro

200 205 210

ttt gaa ggc gag tcc cct ttc gcc gtg gcc tac caa cac gtc cag gaa 787

Phe Glu Gly Glu Ser Pro Phe Ala Val Ala Tyr Gln His Val Gln Glu

215 220 225

gac ccc acc cct cct tcg gat ttc atc gcg gac ctc acc ccg acc tct 835

Asp Pro Thr Pro Pro Ser Asp Phe Ile Ala Asp Leu Thr Pro Thr Ser

230 235 240 245

gct gtc aac gtg gat gcc gtg gta ctc acc gcc atg gca aaa cac ccc 883

Ala Val Asn Val Asp Ala Val Val Leu Thr Ala Met Ala Lys His Pro

250 255 260

gcc gac cgc tac caa aca gcc tcc gaa atg gcc gct gac ctg ggc cgg 931

Ala Asp Arg Tyr Gln Thr Ala Ser Glu Met Ala Ala Asp Leu Gly Arg

265 270 275

cta tcc cgc aat gca gtc tcc cat gcc gca cgc gcg cat gta gaa aca 979

Leu Ser Arg Asn Ala Val Ser His Ala Ala Arg Ala His Val Glu Thr

280 285 290

gaa gaa acc cca gaa gag ccc gaa act cgc ttc tcg acg cgc acc tcc 1027

Glu Glu Thr Pro Glu Glu Pro Glu Thr Arg Phe Ser Thr Arg Thr Ser

295 300 305

acc caa gtg gcc ccc gcc gca ggc gtg gct gcg gcc agt acg ggg tca 1075

Thr Gln Val Ala Pro Ala Ala Gly Val Ala Ala Ala Ser Thr Gly Ser

310 315 320 25

ggg tct tct tcg cgt aaa cgt gga tcc aga ggc ctc acc gcc ctg gcc 1123

Gly Ser Ser Ser Arg Lys Arg Gly Ser Arg Gly Leu Thr Ala Leu Ala

330 335 340

atc gtg tta tcc cta ggt gtc gtc ggc gtt gcc ggt gcc ttc acc tac 1171

Ile Val Leu Ser Leu Gly Val Val Gly Val Ala Gly Ala Phe Thr Tyr

345 350 355

gac tac ttt gcc aac agc tcc tcc act gca acc agc gcg atc ccc aat 1219

Asp Tyr Phe Ala Asn Ser Ser Ser Thr Ala Thr Ser Ala Ile Pro Asn

360 365 370

gtg gaa ggc ctc ccg cag caa gaa gct ctc aca gaa ctt caa gca gca 1267

Val Glu Gly Leu Pro Gln Gln Glu Ala Leu Thr Glu Leu Gln Ala Ala

375 380 385

gga ttt gtt gtc aac atc gtc gaa gaa gcc agc gcc gac gtc gcc gaa 1315

Gly Phe Val Val Asn Ile Val Glu Glu Ala Ser Ala Asp Val Ala Glu

390 395 400 405

ggc ctc gtc atc cga gca aac cca agc gtt gga tcc gaa atc cgc caa 1363

Gly Leu Val Ile Arg Ala Asn Pro Ser Val Gly Ser Glu Ile Arg Gln

410 415 420

ggg gcc acc gtc acc atc acc gtg tcc acc ggc cga gaa atg atc aac 141l

Gly Ala Thr Val Thr Ile Thr Val Ser Thr Gly Arg Glu Met Ile Asn

425 430 435

atc cca gac gtc tcc ggc atg aca ctt gag gac gcc gcc cgc gcc ctc 1459

Ile Pro Asp Val Ser Gly Met Thr Leu Glu Asp Ala Ala Arg Ala Leu

440 445 450

gaa gac gtt ggt ctc ata ctc aac caa aac gtt cgg gaa gaa acc tcc 1507

Glu Asp Val Gly Leu Ile Leu Asn Gln Asn Val Arg Glu Glu Thr Ser

455 460 465

gac gac gtc gaa tct ggc ctc gtc atc gac caa aac ccc gaa gcc ggc 1555

Asp Asp Val Glu Ser Gly Leu Val Ile Asp Gln Asn Pro Glu Ala Gly

470 475 480 485

caa gaa gta gtc gtg ggt tcc tct gta tct cta acc atg tct tca ggc 1603

Gln Glu Val Val Val Gly Ser Ser Val Ser Leu Thr Met Ser Ser Gly

490 495 500

acc gag agc atc cga gtg ccc aac ctc acc ggc atg aac tgg tca caa 1651

Thr Glu Ser Ile Arg Val Pro Asn Leu Thr Gly Met Asn Trp Ser Gln

505 510 515

gca gaa caa aac ctc atc tcc atg ggc ttt aac ccc aca gct tcc tac 1699

Ala Glu Gln Asn Leu Ile Ser Met Gly Phe Asn Pro Thr Ala Ser Tyr

520 525 530

tta gac agc agc gaa cca gaa ggc gaa gtc ctc tca gtt tcc agc caa 1747

Leu Asp Ser Ser Glu Pro Glu Gly Glu Val Leu Ser Val Ser Ser Gln

535 540 545

gga act gaa cta ccc aag ggt tca tcc atc aca gtg gaa gtc tcc aac 1795

Gly Thr Glu Leu Pro Lys Gly Ser Ser Ile Thr Val Glu Val Ser Asn

550 555 560 565

ggc atg ctc atc caa gcc ccc gat ctc gcc cgc atg tcc acc gaa cag 1843

Gly Met Leu Ile Gln Ala Pro Asp Leu Ala Arg Met Ser Thr Glu Gln

570 575 580

gcc atc agt gcc ctc cgc gct gct ggc tgg acc gcc cca gat caa tcc 1891

Ala Ile Ser Ala Leu Arg Ala Ala Gly Trp Thr Ala Pro Asp Gln Ser

585 590 595

ctg atc gtc ggc gac ccc atc cac acc gca gcc ctc gtg gat caa aac 1939

Leu Ile Val Gly Asp Pro Ile His Thr Ala Ala Leu Val Asp Gln Asn

600 605 610

aaa atc gga ttc caa tcc cca acc cct gca acc ctc ttc cgc aaa gac 1987

Lys Ile Gly Phe Gln Ser Pro Thr Pro Ala Thr Leu Phe Arg Lys Asp

615 620 625

gcc caa gtg caa gtg cga ctc ttc gaa ttc gat ctc gct gca ctc gtg 2035

Ala Gln Val Gln Val Arg Leu Phe Glu Phe Asp Leu Ala Ala Leu Val

630 635 640 645

caa tagccaacaa ggaaaccgtc aaggtagctg 2068

Gln

<210>32

<211>646

<212>PRT

<213>谷氨酸棒状杆菌

<400>32

Val Thr Phe Val Ile Ala Asp Arg Tyr Glu Leu Asp Ala Val Ile Gly

1 5 10 15

Ser Gly Gly Met Ser Glu Val Phe Ala Ala Thr Asp Thr Leu Ile Gly

20 25 30

Arg Glu Val Ala Val Lys Met Leu Arg Ile Asp Leu Ala Lys Asp Pro

35 40 45

Asn Phe Arg Glu Arg Phe Arg Arg Glu Ala Gln Asn Ser Gly Arg Leu

50 55 60

Ser His Ser Ser Ile Val Ala Val Phe Asp Thr Gly Glu Val Asp Lys

65 70 75 80

Asp Gly Thr Ser Val Pro Tyr Ile Val Met Glu Arg Val Gln Gly Arg

85 90 95

Asn Leu Arg Glu Val Val Thr Glu Asp Gly Val Phe Thr Pro Val Glu

100 105 110

Ala Ala Asn Ile Leu Ile Pro Val Cys Glu Ala Leu Gln Ala Ser His

115 120 125

Asp Ala Gly Ile Ile His Arg Asp Val Lys Pro Ala Asn Ile Met Ile

130 135 140

Thr Asn Thr Gly Gly Val Lys Val Met Asp Phe Gly Ile Ala Arg Ala

145 150 155 160

Val Asn Asp Ser Thr Ser Ala Met Thr Gln Thr Ser Ala Val Ile Gly

165 170 175

Thr Ala Gln Tyr Leu Ser Pro Glu Gln Ala Arg Gly Lys Pro Ala Asp

180 185 190

Ala Arg Ser Asp Ile Tyr Ala Thr Gly Cys Val Met Tyr Glu Leu Val

195 200 205

Thr Gly Lys Pro Pro Phe Glu Gly Glu Ser Pro Phe Ala Val Ala Tyr

210 215 220

Gln His Val Gln Glu Asp Pro Thr Pro Pro Ser Asp Phe Ile Ala Asp

225 230 235 240

Leu Thr Pro Thr Ser Ala Val Asn Val Asp Ala Val Val Leu Thr Ala

245 250 255

Met Ala Lys His Pro Ala Asp Arg Tyr Gln Thr Ala Ser Glu Met Ala

260 265 270

Ala Asp Leu Gly Arg Leu Ser Arg Asn Ala Val Ser His Ala Ala Arg

275 280 285

Ala His Val Glu Thr Glu Glu Thr Pro Glu Glu Pro Glu Thr Arg Phe

290 295 300

Ser Thr Arg Thr Ser Thr Gln Val Ala Pro Ala Ala Gly Val Ala Ala

305 310 315 320

Ala Ser Thr Gly Ser Gly Ser Ser Ser Arg Lys Arg Gly Ser Arg Gly

325 330 335

Leu Thr Ala Leu Ala Ile Val Leu Ser Leu Gly Val Val Gly Val Ala

340 345 350

Gly Ala Phe Thr Tyr Asp Tyr Phe Ala Asn Ser Ser Ser Thr Ala Thr

355 360 365

Ser Ala Ile Pro Asn Val Glu Gly Leu Pro Gln Gln Glu Ala Leu Thr

370 375 380

Glu Leu Gln Ala Ala Gly Phe Val Val Asn Ile Val Glu Glu Ala Ser

385 390 395 400

Ala Asp Val Ala Glu Gly Leu Val Ile Arg Ala Asn Pro Ser Val Gly

405 410 415

Ser Glu Ile Arg Gln Gly Ala Thr Val Thr Ile Thr Val Ser Thr Gly

420 425 430

Arg Glu Met Ile Asn Ile Pro Asp Val Ser Gly Met Thr Leu Glu Asp

435 440 445

Ala Ala Arg Ala Leu Glu Asp Val Gly Leu Ile Leu Asn Gln Asn Val

450 455 460

Arg Glu Glu Thr Ser Asp Asp Val Glu Ser Gly Leu Val Ile Asp Gln

465 470 475 480

Asn Pro Glu Ala Gly Gln Glu Val Val Val Gly Ser Ser Val Ser Leu

485 490 495

Thr Met Ser Ser Gly Thr Glu Ser Ile Arg Val Pro Asn Leu Thr Gly

500 505 510

Met Asn Trp Ser Gln Ala Glu Gln Asn Leu Ile Ser Met Gly Phe Asn

515 520 525

Pro Thr Ala Ser Tyr Leu Asp Ser Ser Glu Pro Glu Gly Glu Val Leu

530 535 540

Ser Val Ser Ser Gln Gly Thr Glu Leu Pro Lys Gly Ser Ser Ile Thr

545 550 555 560

Val Glu Val Ser Asn Gly Met Leu Ile Gln Ala Pro Asp Leu Ala Arg

565 570 575

Met Ser Thr Glu Gln Ala Ile Ser Ala Leu Arg Ala Ala Gly Trp Thr

580 585 590

Ala Pro Asp Gln Ser Leu Ile Val Gly Asp Pro Ile His Thr Ala Ala

595 600 605

Leu Val Asp Gln Asn Lys Ile Gly Phe Gln Ser Pro Thr Pro Ala Thr

610 615 620

Leu Phe Arg Lys Asp Ala Gln Val Gln Val Arg Leu Phe Glu Phe Asp

625 630 635 640

Leu Ala Ala Leu Val Gln

645

<210>33

<211>3502

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3472)

<223>RXA02097

<400>33

ttggctacag atgtttcagc gcttgcagtg gggtagtgtg tttaaacatc acaattagtt 60

ctagaggaaa acgcattttt tgcgcgggga gagtgtatac atg ccg gct ggc atc 115

Met Pro Ala Gly Ile

1 5

gca gac atg aca gat tca ttg ctc gga tgg gca tca caa act gag ctg 163

Ala Asp Met Thr Asp Ser Leu Leu Gly Trp Ala Ser Gln Thr Glu Leu

10 15 20

gat ctg aac cag cgt ctt gca ggg gta gag tac ttt cca caa att cag 211

Asp Leu Asn Gln Arg Leu Ala Gly Val Glu Tyr Phe Pro Gln Ile Gln

25 30 35

ctg cga cac gat gag ctc gag cgc att cat cgg ttt tac ggc acc ttt 259

Leu Arg His Asp Glu Leu Glu Arg Ile His Arg Phe Tyr Gly Thr Phe

40 45 50

ttg tcc cgc cag gta ggc gcg ggc gca agc ctt ggg gat ctt ttt gaa 307

Leu Ser Arg Gln Val Gly Ala Gly Ala Ser Leu Gly Asp Leu Phe Glu

55 60 65

atg acc cca tgc ctg aca gtc acc acc ttg gtg tct cgg gcg tca cgg 355

Met Thr Pro Cys Leu Thr Val Thr Thr Leu Val Ser Arg Ala Ser Arg

70 75 80 85

atc agc gat cca gca gat ttc ttc ggt gaa tac atc gga gga ctg gga 403

Ile Ser Asp Pro Ala Asp Phe Phe Gly Glu Tyr Ile Gly Gly Leu Gly

90 95 100

ctt agc gca gaa cac gca gca gtt gtt gaa ggg ttg acc gaa aag ctc 451

Leu Ser Ala Glu His Ala Ala Val Val Glu Gly Leu Thr Glu Lys Leu

105 110 115

ttc gca cag gct ggc ctg ctc gtt cct gag gga att gca tct cca ttg 499

Phe Ala Gln Ala Gly Leu Leu Val Pro Glu Gly Ile Ala Ser Pro Leu

120 125 130

gag ttg tta tcc atc cac gca ggc att agt aac cac gaa gtg gcc gca 547

Glu Leu Leu Ser Ile His Ala Gly Ile Ser Asn His Glu Val Ala Ala

135 140 145

gtg ctg acc gaa gtg gaa aac ggc acc acc gaa tat cca ttc atg ttc 595

Val Leu Thr Glu Val Glu Asn Gly Thr Thr Glu Tyr Pro Phe Met Phe

150 155 160 165

gac gct gtc ctg cgc cta acc cct gag tgg gca cag acc ctt atc ggc 643

Asp Ala Val Leu Arg Leu Thr Pro Glu Trp Ala Gln Thr Leu Ile Gly

170 175 180

gga gtt caa gaa ctc att gaa ttt gcc acc acc cac cga act tct tgg 691

Gly Val Gln Glu Leu Ile Glu Phe Ala Thr Thr His Arg Thr Ser Trp

185 190 195

tca gac cgc cag cgc gaa tcc tca ctg cca gcc atg atc gat gag atc 739

Ser Asp Arg Gln Arg Glu Ser Ser Leu Pro Ala Met Ile Asp Glu Ile

200 205 210

gtt gtg gcg gaa ctt cgg gaa cgc cca gtt ggt act gcc gac cgt gaa 787

Val Val Ala Glu Leu Arg Glu Arg Pro Val Gly Thr Ala Asp Arg Glu

215 220 225

aac tcc gtt ggt gtg gca ctt cgt gag ctt cgc cca cgc ctc atc ctg 835

Asn Ser Val Gly Val Ala Leu Arg Glu Leu Arg Pro Arg Leu Ile Leu

230 235 240 45

gat gca gaa cgc cgc aaa gtc tgc ctg cgt cta cct gaa cag cgc gtc 883

Asp Ala Glu Arg Arg Lys Val Cys Leu Arg Leu Pro Glu Gln Arg Val

250 255 260

agc gac gat gaa atc aac tgg cga gtc agc cta gaa ggc acc acc cgg 931

Ser Asp Asp Glu Ile Asn Trp Arg Val Ser Leu Glu Gly Thr Thr Arg

265 270 275

att ttc tcc acc cgc cga gca tgg ggc gat act tct gga tac tcc gaa 979

Ile Phe Ser Thr Arg Arg Ala Trp Gly Asp Thr Ser Gly Tyr Ser Glu

280 285 290

gcc ctc gac atc act gtc gag cgt caa atc cgc gaa acc acc gtc acc 1027

Ala Leu Asp Ile Thr Val Glu Arg Gln Ile Arg Glu Thr Thr Val Thr

295 300 305

gac acc tca aac caa atc acc tgg gtt gtc cca gtc gtg gac ttc aac 1075

Asp Thr Ser Asn Gln Ile Thr Trp Val Val Pro Val Val Asp Phe Asn

310 315 320 325

gac cca gtg ctg gtg ttt tcc gcg cgc ggt gaa aac ctc acc gac aag 1123

Asp Pro Val Leu Val Phe Ser Ala Arg Gly Glu Asn Leu Thr Asp Lys

330 335 340

gtc tcc ctg cac cat caa gag att tac gtt ctc gcg cca gcg gaa gca 1171

Val Ser Leu His His Gln Glu Ile Tyr Val Leu Ala Pro Ala Glu Ala

345 350 355

aaa ctc gaa gac atg gtc act ggc cag cca gta cca gtt att gag caa 1219

Lys Leu Glu Asp Met Val Thr Gly Gln Pro Val Pro Val Ile Glu Gln

360 365 370

ttc ctc gta gag ggc tgg aac tca tgg gtg tgc tcc cgc gtg gac gcc 1267

Phe Leu Val Glu Gly Trp Asn Ser Trp Val Cys Ser Arg Val Asp Ala

375 380 385

cgt ggc ctg tcc tct ctg aag gtc aac aaa gaa gtc cga tgc att gac 1315

Arg Gly Leu Ser Ser Leu Lys Val Asn Lys Glu Val Arg Cys Ile Asp

390 395 400 405

cca cgt cga cgc gtt gcc ttc cac cac cca gcc gaa ttg gtc cct cac 1363

Pro Arg Arg Arg Val Ala Phe His His Pro Ala Glu Leu Val Pro His

410 415 420

gta cga tcc att tcc gga ctc ccc gta cac gcg cag tcc ctg atc gcc 1411

Val Arg Ser Ile Ser Gly Leu Pro Val His Ala Gln Ser Leu Ile Ala

425 430 435

gag ttc cca cca acc ctg agc gga caa gac gaa acc tgg atg ctc tcc 1459

Glu Phe Pro Pro Thr Leu Ser Gly Gln Asp Glu Thr Trp Met Leu Ser

440 445 450

atc tcg gct ttc gca ggt gta ggc gct gct ggt gaa gaa atc gcc gag 1507

Ile Ser Ala Phe Ala Gly Val Gly Ala Ala Gly Glu Glu Ile Ala Glu

455 460 465

cca gag cct ttg gaa gtc cct gcc gac ggt ggc ctt ttc gcc atc ttc 1555

Pro Glu Pro Leu Glu Val Pro Ala Asp Gly Gly Leu Phe Ala Ile Phe

470 475 480 485

gac cca gaa ata tac gac gcc cca tgg gtg ggt gaa tac ctg gtc cga 1603

Asp Pro Glu Ile Tyr Asp Ala Pro Trp Val Gly Glu Tyr Leu Val Arg

490 495 500

ctc cgc ggc cca cgc aat gaa tcc ttc cga ccc gaa ttc gcc atc gtc 1651

Leu Arg Gly Pro Arg Asn Glu Ser Phe Arg Pro Glu Phe Ala Ile Val

505 510 515

gaa gac atg acc acc gaa ttc gaa gtc gcc tca ggt gca tca ttt cga 1699

Glu Asp Met Thr Thr Glu Phe Glu Val Ala Ser Gly Ala Ser Phe Arg

520 525 530

atc cca acc acc act ggt ctc agc gaa gcc agc cta cgc gtg cgt tcc 1747

Ile Pro Thr Thr Thr Gly Leu Ser Glu Ala Ser Leu Arg Val Arg Ser

535 540 545

ggt gaa aag cac ttc acc gca gag cca cgc ctg gtc acc gtt gaa gca 1795

Gly Glu Lys His Phe Thr Ala Glu Pro Arg Leu Val Thr Val Glu Ala

550 555 560 565

acc gac ccc aac gca tca ttc gtg gtc acc acc gat gaa ggc gat caa 1843

Thr Asp Pro Asn Ala Ser Phe Val Val Thr Thr Asp Glu Gly Asp Gln

570 575 580

atg cca ttg cga ttt gtg cca cca caa atc gcc atc gaa ctt cca ctg 1891

Met Pro Leu Arg Phe Val Pro Pro Gln Ile Ala Ile Glu Leu Pro Leu

585 590 595

acc acc gag cca cca acc tgg cgc gtc acc cgt act gtc tgt gga cca 1939

Thr Thr Glu Pro Pro Thr Trp Arg Val Thr Arg Thr Val Cys Gly Pro

600 605 610

cgc gac ctc gac ggt gca ggc gaa ctc cgc atc cgc acc ggt gtc gat 1987

Arg Asp Leu Asp Gly Ala Gly Glu Leu Arg Ile Arg Thr Gly Val Asp

615 620 625

gtc ggc gat cca aag gtc agt gtg cgc aac cac cac ggt tca cca ctg 2035

Val Gly Asp Pro Lys Val Ser Val Arg Asn His His Gly Ser Pro Leu

630 635 640 645

cga acc gtg aaa atg gtc acc cct gac aac ggc cgt acc tgg att gcc 2083

Arg Thr Val Lys Met Val Thr Pro Asp Asn Gly Arg Thr Trp Ile Ala

650 655 660

agc atg aag gaa atc gca gcc agt acc ttt gtg atg cca cgc gga tcc 2131

Ser Met Lys Glu Ile Ala Ala Ser Thr Phe Val Met Pro Arg Gly Ser

665 670 675

atc gaa ttt gag tgg act gac cgc aag gtt gac cgt cgc gtt tcc gtg 2179

Ile Glu Phe Glu Trp Thr Asp Arg Lys Val Asp Arg Arg Val Ser Val

680 685 690

acg att gct gtc att gac aaa act gag aac ttt act ggc atc acc atc 2227

Thr Ile Ala Val Ile Asp Lys Thr Glu Asn Phe Thr Gly Ile Thr Ile

695 700 705

gaa gat gga aag ctc gta ttc gaa gaa ctc gca gcc ggt cgc caa ctc 2275

Glu Asp Gly Lys Leu Val Phe Glu Glu Leu Ala Ala Gly Arg Gln Leu

710 715 720 725

gct gca tgg gtg tgg cca caa acc gca ccg tgg gta agc gca gtg gaa 2323

Ala Ala Trp Val Trp Pro Gln Thr Ala Pro Trp Val Ser Ala Val Glu

730 735 740

ctt gct gtc acc gga cca gag ctg gaa ctc cct gaa gtt ctc gtc ggc 2371

Leu Ala Val Thr Gly Pro Glu Leu Glu Leu Pro Glu Val Leu Val Gly

745 750 755

gca ggc aac ctg att gtt caa ctc cac acc gct gac cca ttc act acc 2419

Ala Gly Asn Leu Ile Val Gln Leu His Thr Ala Asp Pro Phe Thr Thr

760 765 770

tcc gtg acc cca ctg tca cca gga aaa gct gcg gtc acc gtt gag caa 2467

Ser Val Thr Pro Leu Ser Pro Gly Lys Ala Ala Val Thr Val Glu Gln

775 780 785

gaa ggc tac tac tca gca caa acc gaa gaa tat gca cag ctt tca gca 2515

Glu Gly Tyr Tyr Ser Ala Gln Thr Glu Glu Tyr Ala Gln Leu Ser Ala

790 795 800 805

ttc ttc ggt ggg gaa gta gaa gaa cca cca atc agt gac gct gtg gtc 2563

Phe Phe Gly Gly Glu Val Glu Glu Pro Pro Ile Ser Asp Ala Val Val

810 815 820

ccc gca ctt tgg gat gtt tcc cat atc tgg acc gaa cag gga aac acc 2611

Pro Ala Leu Trp Asp Val Ser His Ile Trp Thr Glu Gln Gly Asn Thr

825 830 835

gag cat ctt cca gta gtc cat gcc gcc ctg cgc tcc tca cca gcc gca 2659

Glu His Leu Pro Val Val His Ala Ala Leu Arg Ser Ser Pro Ala Ala

840 845 850

gca ctg aag ggt ctg tcc gct tcg ctg gtt ccc gca cag gca cta cct 2707

Ala Leu Lys Gly Leu Ser Ala Ser Leu Val Pro Ala Gln Ala Leu Pro

855 860 865

gga aaa gtc att tcc tcc gga ctg gca gcc tca ccg ttc acc acg gaa 2755

Gly Lys Val Ile Ser Ser Gly Leu Ala Ala Ser Pro Phe Thr Thr Glu

870 875 880 885

tca cca gca aca gaa gtg cac cgc acc gca tgg atc gga acc ctg caa 2803

Ser Pro Ala Thr Glu Val His Arg Thr Ala Trp Ile Gly Thr Leu Gln

890 895 900

ctc ctg ggt gca ctg cca agc gca ttc aag gaa gcc gaa gag ctt ggc 2851

Leu Leu Gly Ala Leu Pro Ser Ala Phe Lys Glu Ala Glu Glu Leu Gly

905 910 915

aac cgc aca cca ctg ctg cca atc ctc gga caa ctt gag gaa gtc gcc 2899

Asn Arg Thr Pro Leu Leu Pro Ile Leu Gly Gln Leu Glu Glu Val Ala

920 925 930

ggc aag aac atc ctg tcc acc ctt gca act ggc cgt gac tcc act ttg 2947

Gly Lys Asn Ile Leu Ser Thr Leu Ala Thr Gly Arg Asp Ser Thr Leu

935 940 945

gac acc gca tgc atc gac caa tcc acc gtt gcg att gcc ggc atg aac 2995

Asp Thr Ala Cys Ile Asp Gln Ser Thr Val Ala Ile Ala Gly Met Asn

950 955 960 965

gaa acc cag caa aaa gcc ctg ctg gac atg ttc ttc agc aac gcc gac 3043

Glu Thr Gln Gln Lys Ala Leu Leu Asp Met Phe Phe Ser Asn Ala Asp

970 975 980

atc gtt cct gga cca cta atg gaa gac aac acc cgc ctc atg gca gtg 3091

Ile Val Pro Gly Pro Leu Met Glu Asp Asn Thr Arg Leu Met Ala Val

985 990 995

ttc gaa acc ttc aag aag cgc gat gca ctc cgt gag gtt ctc cag act 3139

Phe Glu Thr Phe Lys Lys Arg Asp Ala Leu Arg Glu Val Leu Gln Thr

1000 1005 1010

gaa ggc ttg att aag acc gct gta gaa ctt ctt cgt gcc atg cgt gga 3187

Glu Gly Leu Ile Lys Thr Ala Val Glu Leu Leu Arg Ala Met Arg Gly

1015 1020 1025

acc cag cgt cag ctg tat tcc tcc gca cgt att cga ttc gac aag ctc 3235

Thr Gln Arg Gln Leu Tyr Ser Ser Ala Arg Ile Arg Phe Asp Lys Leu

1030 1035 1040 1045

gat ggt gtc aac act gac aac cca gaa aac atg tgg gca ctc acc cca 3283

Asp Gly Val Asn Thr Asp Asn Pro Glu Asn Met Trp Ala Leu Thr Pro

1050 1055 1060

gtt gtg tca ctg gtg ttc gcg ttg tca tcc cgt ttg cat gca cac gaa 3331

Val Val Ser Leu Val Phe Ala Leu Ser Ser Arg Leu His Ala His Glu

1065 1070 1075

ttg atc ggc aag acc cga act ctc gat cgt gca tct gcc ggt tgg ggt 3379

Leu Ile Gly Lys Thr Arg Thr Leu Asp Arg Ala Ser Ala Gly Trp Gly

1080 1085 1090

cga atc gct gat ctg gtg cca gac ctt gtc acc ggt gac ttg atc tcc 3427

Arg Ile Ala Asp Leu Val Pro Asp Leu Val Thr Gly Asp Leu Ile Ser

1095 1100 1105

gcg gag gca atg gtt ttg gga gct cga aac cca gga ctc gtc gat 3472

Ala Glu Ala Met Val Leu Gly Ala Arg Asn Pro Gly Leu Val Asp

1110 1115 1120

tagtccctga tttcatcgga gggcacctag 3502

<210>34

<211>1124

<212>PRT

<213>谷氨酸棒状杆菌

<400>34

Met Pro Ala Gly Ile Ala Asp Met Thr Asp Ser Leu Leu Gly Trp Ala

1 5 10 15

Ser Gln Thr Glu Leu Asp Leu Asn Gln Arg Leu Ala Gly Val Glu Tyr

20 25 30

Phe Pro Gln Ile Gln Leu Arg His Asp Glu Leu Glu Arg Ile His Arg

35 40 45

Phe Tyr Gly Thr Phe Leu Ser Arg Gln Val Gly Ala Gly Ala Ser Leu

50 55 60

Gly Asp Leu Phe Glu Met Thr Pro Cys Leu Thr Val Thr Thr Leu Val

65 70 75 80

Ser Arg Ala Ser Arg Ile Ser Asp Pro Ala Asp Phe Phe Gly Glu Tyr

85 90 95

Ile Gly Gly Leu Gly Leu Ser Ala Glu His Ala Ala Val Val Glu Gly

100 105 110

Leu Thr Glu Lys Leu Phe Ala Gln Ala Gly Leu Leu Val Pro Glu Gly

115 120 125

Ile Ala Ser Pro Leu Glu Leu Leu Ser Ile His Ala Gly Ile Ser Asn

130 135 140

His Glu Val Ala Ala Val Leu Thr Glu Val Glu Asn Gly Thr Thr Glu

145 150 155 160

Tyr Pro Phe Met Phe Asp Ala Val Leu Arg Leu Thr Pro Glu Trp Ala

165 170 175

Gln Thr Leu Ile Gly Gly Val Gln Glu Leu Ile Glu Phe Ala Thr Thr

180 185 190

His Arg Thr Ser Trp Ser Asp Arg Gln Arg Glu Ser Ser Leu Pro Ala

195 200 205

Met Ile Asp Glu Ile Val Val Ala Glu Leu Arg Glu Arg Pro Val Gly

210 215 220

Thr Ala Asp Arg Glu Asn Ser Val Gly Val Ala Leu Arg Glu Leu Arg

225 230 235 240

Pro Arg Leu Ile Leu Asp Ala Glu Arg Arg Lys Val Cys Leu Arg Leu

245 250 255

Pro Glu Gln Arg Val Ser Asp Asp Glu Ile Asn Trp Arg Val Ser Leu

260 265 270

Glu Gly Thr Thr Arg Ile Phe Ser Thr Arg Arg Ala Trp Gly Asp Thr

275 280 285

Ser Gly Tyr Ser Glu Ala Leu Asp Ile Thr Val Glu Arg Gln Ile Arg

290 295 300

Glu Thr Thr Val Thr Asp Thr Ser Asn Gln Ile Thr Trp Val Val Pro

305 310 315 320

Val Val Asp Phe Asn Asp Pro Val Leu Val Phe Ser Ala Arg Gly Glu

325 330 335

Asn Leu Thr Asp Lys Val Ser Leu His His Gln Glu Ile Tyr Val Leu

340 345 350

Ala Pro Ala Glu Ala Lys Leu Glu Asp Met Val Thr Gly Gln Pro Val

355 360 365

Pro Val Ile Glu Gln Phe Leu Val Glu Gly Trp Asn Ser Trp Val Cys

370 375 380

Ser Arg Val Asp Ala Arg Gly Leu Ser Ser Leu Lys Val Asn Lys Glu

385 390 395 400

Val Arg Cys Ile Asp Pro Arg Arg Arg Val Ala Phe His His Pr0 Ala

405 410 415

Glu Leu Val Pro His Val Arg Ser Ile Ser Gly Leu Pro Val His Ala

420 425 430

Gln Ser Leu Ile Ala Glu Phe Pro Pro Thr Leu Ser Gly Gln Asp Glu

435 440 445

Thr Trp Met Leu Ser Ile Ser Ala Phe Ala Gly Val Gly Ala Ala Gly

450 455 460

Glu Glu Ile Ala Glu Pro Glu Pro Leu Glu Val Pro Ala Asp Gly Gly

465 470 475 480

Leu Phe Ala Ile Phe Asp Pro Glu Ile Tyr Asp Ala Pro Trp Val Gly

485 490 495

Glu Tyr Leu Val Arg Leu Arg Gly Pro Arg Asn Glu Ser Phe Arg Pro

500 505 510

Glu Phe Ala Ile Val Glu Asp Met Thr Thr Glu Phe Glu Val Ala Ser

515 520 525

Gly Ala Ser Phe Arg Ile Pro Thr Thr Thr Gly Leu Ser Glu Ala Ser

530 535 540

Leu Arg Val Arg Ser Gly Glu Lys His Phe Thr Ala Glu Pro Arg Leu

545 550 555 560

Val Thr Val Glu Ala Thr Asp Pro Asn Ala Ser Phe Val Val Thr Thr

565 570 575

Asp Glu Gly Asp Gln Met Pro Leu Arg Phe Val Pro Pro Gln Ile Ala

580 585 590

Ile Glu Leu Pro Leu Thr Thr Glu Pro Pro Thr Trp Arg Val Thr Arg

595 600 605

Thr Val Cys Gly Pro Arg Asp Leu Asp Gly Ala Gly Glu Leu Arg Ile

610 615 620

Arg Thr Gly Val Asp Val Gly Asp Pro Lys Val Ser Val Arg Asn His

625 630 635 640

His Gly Ser Pro Leu Arg Thr Val Lys Met Val Thr Pro Asp Asn Gly

645 650 655

Arg Thr Trp Ile Ala Ser Met Lys Glu Ile Ala Ala Ser Thr Phe Val

660 665 670

Met Pro Arg Gly Ser Ile Glu Phe Glu Trp Thr Asp Arg Lys Val Asp

675 680 685

Arg Arg Val Ser Val Thr Ile Ala Val Ile Asp Lys Thr Glu Asn Phe

690 695 700

Thr Gly Ile Thr Ile Glu Asp Gly Lys Leu Val Phe Glu Glu Leu Ala

705 710 715 720

Ala Gly Arg Gln Leu Ala Ala Trp Val Trp Pro Gln Thr Ala Pro Trp

725 730 735

Val Ser Ala Val Glu Leu Ala Val Thr Gly Pro Glu Leu Glu Leu Pro

740 745 750

Glu Val Leu Val Gly Ala Gly Asn Leu Ile Val Gln Leu His Thr Ala

755 760 765

Asp Pro Phe Thr Thr Ser Val Thr Pro Leu Ser Pro Gly Lys Ala Ala

770 775 780

Val Thr Val Glu Gln Glu Gly Tyr Tyr Ser Ala Gln Thr Glu Glu Tyr

785 790 795 800

Ala Gln Leu Ser Ala Phe Phe Gly Gly Glu Val Glu Glu Pro Pro Ile

805 810 815

Ser Asp Ala Val Val Pro Ala Leu Trp Asp Val Ser His Ile Trp Thr

820 825 830

Glu Gln Gly Asn Thr Glu His Leu Pro Val Val His Ala Ala Leu Arg

835 840 845

Ser Ser Pro Ala Ala Ala Leu Lys Gly Leu Ser Ala Ser Leu Val Pro

850 855 860

Ala Gln Ala Leu Pro Gly Lys Val Ile Ser Ser Gly Leu Ala Ala Ser

865 870 875 880

Pro Phe Thr Thr Glu Ser Pro Ala Thr Glu Val His Arg Thr Ala Trp

885 890 895

Ile Gly Thr Leu Gln Leu Leu Gly Ala Leu Pro Ser Ala Phe Lys Glu

900 905 910

Ala Glu Glu Leu Gly Asn Arg Thr Pro Leu Leu Pro Ile Leu Gly Gln

915 920 925

Leu Glu Glu Val Ala Gly Lys Asn Ile Leu Ser Thr Leu Ala Thr Gly

930 935 940

Arg Asp Ser Thr Leu Asp Thr Ala Cys Ile Asp Gln Ser Thr Val Ala

945 950 955 960

Ile Ala Gly Met Asn Glu Thr Gln Gln Lys Ala Leu Leu Asp Met Phe

965 970 975

Phe Ser Asn Ala Asp Ile Val Pro Gly Pro Leu Met Glu Asp Asn Thr

980 985 990

Arg Leu Met Ala Val Phe Glu Thr Phe Lys Lys Arg Asp Ala Leu Arg

995 1000 1005

Glu Val Leu Gln Thr Glu Gly Leu Ile Lys Thr Ala Val Glu Leu Leu

1010 1015 1020

Arg Ala Met Arg Gly Thr Gln Arg Gln Leu Tyr Ser Ser Ala Arg Ile

1025 1030 1035 1040

Arg Phe Asp Lys Leu Asp Gly Val Asn Thr Asp Asn Pro Glu Asn Met

1045 1050 1055

Trp Ala Leu Thr Pro Val Val Ser Leu Val Phe Ala Leu Ser Ser Arg

1060 1065 1070

Leu His Ala His Glu Leu Ile Gly Lys Thr Arg Thr Leu Asp Arg Ala

1075 1080 1085

Ser Ala Gly Trp Gly Arg Ile Ala Asp Leu Val Pro Asp Leu Val Thr

1090 1095 1100

Gly Asp Leu Ile Ser Ala Glu Ala Met Val Leu Gly Ala Arg Asn Pro

1105 1110 1115 1120

Gly Leu Val Asp

<210>35

<211>694

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(664)

<223>RXA02210

<400>35

ttcatggcca atgtggtggg gtcctttcta atacgccaaa tttttcaaac ccatcctctt 60

ccatgcgtgt tttcaccgct attttccata ggagtacatt gtg tcc gta gcg gca 115

Val Ser Val Ala Ala

1 5

ggc gac aaa cca aca aat agc cgt caa gaa atc ctc gaa ggt gcc cga 163

Gly Asp Lys Pro Thr Asn Ser Arg Gln Glu Ile Leu Glu Gly Ala Arg

10 15 20

cgg tgc ttc gct gag cac ggc tat gaa ggc gca acc gta cgc cga ctg 211

Arg Cys Phe Ala Glu His Gly Tyr Glu Gly Ala Thr Val Arg Arg Leu

25 30 35

gaa gaa gca aca ggt aaa tca cgc gga gcg atc ttt cat cac ttc ggt 259

Glu Glu Ala Thr Gly Lys Ser Arg Gly Ala Ile Phe His His Phe Gly

40 45 50

gac aaa gaa aac ctg ttc cta gcc ctc gcg cgg gaa gat gca gcc cgc 307

Asp Lys Glu Asn Leu Phe Leu Ala Leu Ala Arg Glu Asp Ala Ala Arg

55 60 65

atg gcg gag gtg gtg tct gaa aat ggc ctc gtt gaa gtg atg cga gga 355

Met Ala Glu Val Val Ser Glu Asn Gly Leu Val Glu Val Met Arg Gly

70 75 80 85

atg ctg gaa gat cct gaa cga tat gac tgg atg tca gta cgc ctg gag 403

Met Leu Glu Asp Pro Glu Arg Tyr Asp Trp Met Ser Val Arg Leu Glu

90 95 100

atc tcc aag cag ctg cgc acc gac ccg gta ttc cgc gca aaa tgg att 451

Ile Ser Lys Gln Leu Arg Thr Asp Pro Val Phe Arg Ala Lys Trp Ile

105 110 115

gat cac caa agt gtt cta gac gaa gct gtc cgc gtg cgt ttg tcc cgc 499

Asp His Gln Ser Val Leu Asp Glu Ala Val Arg Val Arg Leu Ser Arg

120 125 130

aac gtg gat aag gga caa atg cgc act gac gtc ccg atc gaa gtg ctg 547

Asn Val Asp Lys Gly Gln Met Arg Thr Asp Val Pro Ile Glu Val Leu

135 140 145

cac acc ttc tta gag act gtt ctc gac ggt ttc atc tcc cgt ctt gct 595

His Thr Phe Leu Glu Thr Val Leu Asp Gly Phe Ile Ser Arg Leu Ala

150 155 160 165

acc ggc gca tcc aca gaa gga ctg tcc gaa gta ttg gat ctg gtc gag 643

Thr Gly Ala Ser Thr Glu Gly Leu Ser Glu Val Leu Asp Leu Val Glu

170 175 180

gga act gtc cgt aaa cgc gac taaacgaccc ctgattcaca ctttcagact 694

Gly Thr Val Arg Lys Arg Asp

185

<210>36

<211>188

<212>PRT

<213>谷氨酸棒状杆菌

<400>36

Val Ser Val Ala Ala Gly Asp Lys Pro Thr Ash Ser Arg Gln Glu Ile

1 5 10 15

Leu Glu Gly Ala Arg Arg Cys Phe Ala Glu His Gly Tyr Glu Gly Ala

20 25 30

Thr Val Arg Arg Leu Glu Glu Ala Thr Gly Lys Ser Arg Gly Ala Ile

35 40 45

Phe His His Phe Gly Asp Lys Glu Asn Leu Phe Leu Ala Leu Ala Arg

50 55 60

Glu Asp Ala Ala Arg Met Ala Glu Val Val Ser Glu Asn Gly Leu Val

65 70 75 80

Glu Val Met Arg Gly Met Leu Glu Asp Pro Glu Arg Tyr Asp Trp Met

85 90 95

Ser Val Arg Leu Glu Ile Ser Lys Gln Leu Arg Thr Asp Pro Val Phe

100 105 110

Arg Ala Lys Trp Ile Asp His Gln Ser Val Leu Asp Glu Ala Val Arg

115 120 125

Val Arg Leu Ser Arg Asn Val Asp Lys Gly Gln Met Arg Thr Asp Val

130 135 140

Pro Ile Glu Val Leu His Thr Phe Leu Glu Thr Val Leu Asp Gly Phe

145 150 155 160

Ile Ser Arg Leu Ala Thr Gly Ala Ser Thr Glu Gly Leu Ser Glu Val

165 170 175

Leu Asp Leu Val Glu Gly Thr Val Arg Lys Arg Asp

180 185

<210>37

<211>3829

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3799)

<223>RXA02362

<400>37

acaattgttc ccattcgcat atctcgtgtc caccagagtc gctacagtaa cgagaaactt60

aattatttga tccgattctt ccgttcaaag gcctcattcc gtg act atc tct cgc 115

Val Thr Ile Ser Arg

1 5

cga ctc aaa caa gag cgc agt ttc gct gac gat ctt caa gat ctc aaa 163

Arg Leu Lys Gln Glu Arg Ser Phe Ala Asp Asp Leu Gln Asp Leu Lys

10 15 20

act ctc aat gat caa ctg cgg ttt aca aac gcc aaa ttg caa gct cgc 211

Thr Leu Asn Asp Gln Leu Arg Phe Thr Asn Ala Lys Leu Gln Ala Arg

25 30 35

atc agt ggt att ggc aat gat gga aag aaa atc acg cgc cct acc cca 259

Ile Ser Gly Ile Gly Asn Asp Gly Lys Lys Ile Thr Arg Pro Thr Pro

40 45 50

ctc ctt gcg ctg gat ttt cag ctg acc gtt gaa gaa tac gaa acg atc 307

Leu Leu Ala Leu Asp Phe Gln Leu Thr Val Glu Glu Tyr Glu Thr Ile

55 60 65

att gca atc ttg gtg gaa gca gtt ggc gga aat caa tcc aag cca gcg 355

Ile Ala Ile Leu Val Glu Ala Val Gly Gly Asn Gln Ser Lys Pro Ala

70 75 80 85

att ctt aaa gat ctg ttt ata gaa tat cca ctc gtc ttc ctg gca gcg 403

Ile Leu Lys Asp Leu Phe Ile Glu Tyr Pro Leu Val Phe Leu Ala Ala

90 95 100

ctt tct gga acc gcc atg ctc gat gct caa gaa ggt ttc tgg cct gcg 451

Leu Ser Gly Thr Ala Met Leu Asp Ala Gln Glu Gly Phe Trp Pro Ala

105 110 115

ttc tgg aaa cgc act cag gtg tca gtt cea gag cat gta tac gac gcg 499

Phe Trp Lys Arg Thr Gln Val Ser Val Pro Glu His Val Tyr Asp Ala

120 125 130

atc cgt aaa gaa cta gtt aat agc atc cgc aaa aat ggc cta gaa act 547

Ile Arg Lys Glu Leu Val Asn Ser Ile Arg Lys Asn Gly Leu Glu Thr

135 140 145

ttt tct ctc gct gac ctc aat cga cgc gaa tat gtc gga ctc atc caa 595

Phe Ser Leu Ala Asp Leu Asn Arg Arg Glu Tyr Val Gly Leu Ile Gln

150 155 160 165

ctt cac agt ggc ctt tct gca aaa gac atg ctc gcc ttg gtc aaa ttt 643

Leu His Ser Gly Leu Ser Ala Lys Asp Met Leu Ala Leu Val Lys Phe

170 175 180

atc gat cac act cga gca gaa aac caa gga tgg gat tct ggt gag gac 691

Ile Asp His Thr Arg Ala Glu Asn Gln Gly Trp Asp Ser Gly Glu Asp

185 190 195

ttt gca tca tat gcg aag agt gtc ttc tcc tcc ggg gac aac cta tta 739

Phe Ala Ser Tyr Ala Lys Ser Val Phe Ser Ser Gly Asp Asn Leu Leu

200 205 210

acc acg gag tcg ctc aag caa tta gtc acc cac atc cct gcg cgt tcc 787

Thr Thr Glu Ser Leu Lys Gln Leu Val Thr His Ile Pro Ala Arg Ser

215 220 225

gtc gac ttc atc gcc aga gtc tat gaa cta acc aat tgg tac cgc gac 835

Val Asp Phe Ile Ala Arg Val Tyr Glu Leu Thr Asn Trp Tyr Arg Asp

230 235 240 245

ctc aaa gac ctc aat gaa gta gaa gcc ttc gta ggt act cat ggg ctg 883

Leu Lys Asp Leu Asn Glu Val Glu Ala Phe Val Gly Thr His Gly Leu

250 255 260

ccg gaa ttg tct ttc aaa ttt ctt ctg gag tgt ctg agc ggc gaa gct 931

Pro Glu Leu Ser Phe Lys Phe Leu Leu Glu Cys Leu Ser Gly Glu Ala

265 270 275

gaa caa att gcc gaa aag acg aaa gca gca cca gca agc ctg gaa aac 979

Glu Gln Ile Ala Glu Lys Thr Lys Ala Ala Pro Ala Ser Leu Glu Asn

280 285 290

ctg gaa cct ccg cat ctc tat ctg gat cca cag agt ttt gaa ctc agt 1027

Leu Glu Pro Pro His Leu Tyr Leu Asp Pro Gln Ser Phe Glu Leu Ser

295 300 305

ctt gtt ttc cca gcg atc tct aaa act gca gca ctt cag att cca gca 1075

Leu Val Phe Pro Ala Ile Ser Lys Thr Ala Ala Leu Gln Ile Pro Ala

310 315 320 325

cca gaa tgg aca gtg att tat gac gga aac tcc att aaa gtt cgt ccc 1123

Pro Glu Trp Thr Val Ile Tyr Asp Gly Asn Ser Ile Lys Val Arg Pro

330 335 340

gaa cag gac tgg tcc tac gga ggt ttc gcc gaa tac cgt ttg cct tta 1171

Glu Gln Asp Trp Ser Tyr Gly Gly Phe Ala Glu Tyr Arg Leu Pro Leu

345 350 355

gac aaa ccg ctc tcc agc ttg aga gtc atc act cca aca gag aaa tcc 1219

Asp Lys Pro Leu Ser Ser Leu Arg Val Ile Thr Pro Thr Glu Lys Ser

360 365 370

cta att ctg att gaa gga ttt ggc cac aag aat ccc att atg ttc ttt 1267

Leu Ile Leu Ile Glu Gly Phe Gly His Lys Asn Pro Ile Met Phe Phe

375 380 385

aag aac aac ggt cag cca tat gca aac caa gaa atg ctc agt ggc aac 1315

Lys Asn Asn Gly Gln Pro Tyr Ala Asn Gln Glu Met Leu Ser Gly Asn

390 395 400 405

gct gtc aca gct ata gtc cca gct gca gca atc att cgt gca cgt atg 1363

Ala Val Thr Ala Ile Val Pro Ala Ala Ala Ile Ile Arg Ala Arg Met

410 415 420

cga gct tcc aag act ttc aac tat caa gac ttg ggt ccc ttg tcc gga 1411

Arg Ala Ser Lys Thr Phe Asn Tyr Gln Asp Leu Gly Pro Leu Ser Gly

425 430 435

tgg aac aag tgg gtc att cgt tcg atc cca ctc aaa cga gct gaa tcg 1459

Trp Asn Lys Trp Val Ile Arg Ser Ile Pro Leu Lys Arg Ala Glu Ser

440 445 450

atc aca gtc tcc cac ggt ggc ttc aga aaa gaa ctc cca gtt cga cgc 1507

Ile Thr Val Ser His Gly Gly Phe Arg Lys Glu Leu Pro Val Arg Arg

455 460 465

aaa gtt gat gtt caa tgg att act gag gat ctc acg atc gag aat ctt 1555

Lys Val Asp Val Gln Trp Ile Thr Glu Asp Leu Thr Ile Glu Asn Leu

470 475 480 485

caa ggt ctc gat cat gag ccc gtt ttc cac acg agt ccc cgc atc gaa 1603

Gln Gly Leu Asp His Glu Pro Val Phe His Thr Ser Pro Arg Ile Glu

490 495 500

ttc ccc acc tct gga tca aac tgg gta att cag tat tca cag att ctt 1651

Phe Pro Thr Ser Gly Ser Asn Trp Val Ile Gln Tyr Ser Gln Ile Leu

505 510 515

cca gat ggc agc ctc atc gaa atg gaa gat tac cca gtc gaa cct gaa 1699

Pro Asp Gly Ser Leu Ile Glu Met Glu Asp Tyr Pro Val Glu Pro Glu

520 525 530

aac ttc gga tac gaa cta gac ctc ttc gaa gaa tcc gac gac cct tgg 1747

Asn Phe Gly Tyr Glu Leu Asp Leu Phe Glu Glu Ser Asp Asp Pro Trp

535 540 545

gtc ggg caa ttt tta gta act ctg ctc aag gat gaa aaa gtc tat gaa 1795

Val Gly Gln Phe Leu Val Thr Leu Leu Lys Asp Glu Lys Val Tyr Glu

550 555 560 565

acc cgc aaa ttc aat ctc gcg gaa ggc ctc gat ctt tcc cta aca ttc 1843

Thr Arg Lys Phe Asn Leu Ala Glu Gly Leu Asp Leu Ser Leu Thr Phe

570 575 580

agc gga ggc gga cct gaa aat cga ttt agg tac ccc agc atc aat cag 1891

Ser Gly Gly Gly Pro Glu Asn Arg Phe Arg Tyr Pro Ser Ile Asn Gln

585 590 595

gga caa act ggc tta aca aag act ttc gcc cgt ttt agt tcc aat tct 1939

Gly Gln Thr Gly Leu Thr Lys Thr Phe Ala Arg Phe Ser Ser Asn Ser

600 605 610

gaa aag cac atc agg ttc cca gat gag atc atc ggg ctt gat gca ttc 1987

Glu Lys His Ile Arg Phe Pro Asp Glu Ile Ile Gly Leu Asp Ala Phe

615 620 625

acc tct caa aaa gcg ttt aac atc gca agc ggt gat ttc cct gag gac 2035

Thr Ser Gln Lys Ala Phe Asn Ile Ala Ser Gly Asp Phe Pro Glu Asp

630 635 640 645

tac aac ctc gac gtt ttc atc acg cct ccg caa ctt cac tac caa gta 2083

Tyr Asn Leu Asp Val Phe Ile Thr Pro Pro Gln Leu His Tyr Gln Val

650 655 660

cct gtc aca cac agc caa aca aag tgg gaa agc aca aag acg aca cta 2131

Pro Val Thr His Ser Gln Thr Lys Trp Glu Ser Thr Lys Thr Thr Leu

665 670 675

gat ttc aat gac ttt gcc gat gga aac ctc cag atc aga ttc cct aat 2179

Asp Phe Asn Asp Phe Ala Asp Gly Asn Leu Gln Ile Arg Phe Pro Asn

680 685 690

gaa gtc tat gat cca aac ttg aaa atc att aaa atg gtg gca tac aag 2227

Glu Val Tyr Asp Pro Asn Leu Lys Ile Ile Lys Met Val Ala Tyr Lys

695 700 705

aaa cct gag tcc agt gag cct aaa tac tta agc aaa att ggt tca agc 2275

Lys Pro Glu Ser Ser Glu Pro Lys Tyr Leu Ser Lys Ile Gly Ser Ser

710 715 720 725

aaa gtg tgg tct atc cct atg gat cgc atc aag gaa ctc atg gat gat 2323

Lys Val Trp Ser Ile Pro Met Asp Arg Ile Lys Glu Leu Met Asp Asp

730 735 740

gat gcc caa ttc ctt ttg atc gcg gag tgg ttc gct gaa agt aaa gac 2371

Asp Ala Gln Phe Leu Leu Ile Ala Glu Trp Phe Ala Glu Ser Lys Asp

745 750 755

cag cac cga gag aag atc att agc gaa gct aag cga act gga aaa atc 2419

Gln His Arg Glu Lys Ile Ile Ser Glu Ala Lys Arg Thr Gly Lys Ile

760 765 770

tcc aat gca gcg ctt aag agt gct cgt cct caa cct caa gca agt tcc 2467

Ser Asn Ala Ala Leu Lys Ser Ala Arg Pro Gln Pro Gln Ala Ser Ser

775 780 785

cac att gca aca att gag aaa aag ccc cta cta gct gcg gct gaa att 2515

His Ile Ala Thr Ile Glu Lys Lys Pro Leu Leu Ala Ala Ala Glu Ile

790 795 800 805

aag ctt tct acc gtg gag ttg gaa ctt ggt cgg cac act tct aag aga 2563

Lys Leu Ser Thr Val Glu Leu Glu Leu Gly Arg His Thr Ser Lys Arg

810 815 820

ctg gaa ggc tgg gca tgg tct gcg ctc aac ccg ctt gat cca cca atc 2611

Leu Glu Gly Trp Ala Trp Ser Ala Leu Asn Pro Leu Asp Pro Pro Ile

825 830 835

aaa gtc gat ttc caa gga acc tca ggc tca ctt cca gac acc cac ttc 2659

Lys Val Asp Phe Gln Gly Thr Ser Gly Ser Leu Pro Asp Thr His Phe

840 845 850

gtc gtt ggc cct tta atc gtg gaa gtg aga gaa aaa gag ttt ctc tcc 2707

Val Val Gly Pro Leu Ile Val Glu Val Arg Glu Lys Glu Phe Leu Ser

855 860 865

caa tgg cag cca aaa gtt ccc tca gtt aaa gcc gtg gtt gca aat gat 2755

Gln Trp Gln Pro Lys Val Pro Ser Val Lys Ala Val Val Ala Asn Asp

870 875 880 885

ccc tca ttt gaa ttg gac cct caa ttt gat cct ttc ctc aca cac cga 2803

Pro Ser Phe Glu Leu Asp Pro Gln Phe Asp Pro Phe Leu Thr His Arg

890 895 900

tgg atg ttc gct cca cga agt ggg aag gtc tta ctc cca caa gaa atc 2851

Trp Met Phe Ala Pro Arg Ser Gly Lys Val Leu Leu Pro Gln Glu Ile

905 910 915

cgc aca gtg tgg gac gcc cga ttc aat atg cgc cat gtc tta gcg cag 2899

Arg Thr Val Trp Asp Ala Arg Phe Asn Met Arg His Val Leu Ala Gln

920 925 930

cgt gaa aac ctt cat gtg aaa tcg att caa gat ttt gac gat gcc acc 2947

Arg Glu Asn Leu His Val Lys Ser Ile Gln Asp Phe Asp Asp Ala Thr

935 940 945

agt acc tat ctc acc agt gat cct cgg gtg gca tta gat gaa ttg gat 2995

Ser Thr Tyr Leu Thr Ser Asp Pro Arg Val Ala Leu Asp Glu Leu Asp

950 955 960 965

aag agc tca att ccg tct aat tcc cac ttt gaa tca ttc atc cga tcc 3043

Lys Ser Ser Ile Pro Ser Asn Ser His Phe Glu Ser Phe Ile Arg Ser

970 975 980

gga tta gct gag ctt tct ttc gaa gtt gac gac aca gcc gga gat atc 3091

Gly Leu Ala Glu Leu Ser Phe Glu Val Asp Asp Thr Ala Gly Asp Ile

985 990 995

cat cgc gtt ccc tgg atc ggc ctg atc cag gaa atg aac gac ctc aga 3139

His Arg Val Pro Trp Ile Gly Leu Ile Gln Glu Met Asn Asp Leu Arg

1000 1005 1010

att ctg cag ata caa ggc tat gaa aca gaa gaa cga gcc atc gaa cgc 3187

Ile Leu Gln Ile Gln Gly Tyr Glu Thr Glu Glu Arg Ala Ile Glu Arg

1015 1020 1025

cga aat tcg cag agc tac atc cgt gag ata gga ggc agt gaa ttg tgg 3235

Arg Asn Ser Gln Ser Tyr Ile Arg Glu Ile Gly Gly Ser Glu Leu Trp

1030 1035 1040 1045

aat atc cta aaa gga aat tca gag gga ttg tct ctt gct caa aaa tgc 3283

Asn Ile Leu Lys Gly Asn Ser Glu Gly Leu Ser Leu Ala Gln Lys Cys

1050 1055 1060

gca cca caa gcc act gag att aat gtg att cgt aat tca ggc ttg gaa 3331

Ala Pro Gln Ala Thr Glu Ile Asn Val Ile Arg Asn Ser Gly Leu Glu

1065 1070 1075

gct atg cgc aat ggg ctg ggc gcc gat cag ttc agc gcc gag ttt att 3379

Ala Met Arg Asn Gly Leu Gly Ala Asp Gln Phe Ser Ala Glu Phe Ile

1080 1085 1090

tca gca gac tca cgc cta cga gct cag ctt gaa tgg ttg gaa aac cgc 3427

Ser Ala Asp Ser Arg Leu Arg Ala Gln Leu Glu Trp Leu Glu Asn Arg

1095 1100 1105

cga gag ctc aat gat ctc ggc cag ctc cca acg ctc ttc gat ttc gcc 3475

Arg Glu Leu Asn Asp Leu Gly Gln Leu Pro Thr Leu Phe Asp Phe Ala

1110 1115 1120 1125

gag aaa tac gag tac ctc atc gat cac tta ggt gat gat cgc atc aag 3523

Glu Lys Tyr Glu Tyr Leu Ile Asp His Leu Gly Asp Asp Arg Ile Lys

1130 1135 1140

gtc act gca cgt gag ctg tct act ctt gcg tcg gaa cac cgt cgc ggc 3571

Val Thr Ala Arg Glu Leu Ser Thr Leu Ala Ser Glu His Arg Arg Gly

1145 1150 1155

aac gct gaa aac tgg ctt tat gca cca tat gtg tca ttc att tac agc 3619

Asn Ala Glu Asn Trp Leu Tyr Ala Pro Tyr Val Ser Phe Ile Tyr Ser

1160 1165 1170

ttg ctt aac cga atg atc gct cat gaa gta ata cgt ccg atc gct cag 3667

Leu Leu Asn Arg Met Ile Ala His Glu Val Ile Arg Pro Ile Ala Gln

1175 1180 1185

atc aat tac tca cgg cac gat tgg gca aac gct gct cgg ctg att cct 3715

Ile Asn Tyr Ser Arg His Asp Trp Ala Asn Ala Ala Arg Leu Ile Pro

1190 1195 1200 1205

cgt ctc aca gga ttt gac ctg gtg agt gcc gaa gcg aaa gtg ctc agc 3763

Arg Leu Thr Gly Phe Asp Leu Val Ser Ala Glu Ala Lys Val Leu Ser

1210 1215 1220

gca ata aac aac aac aat ata atc cca act gca att taaggatcac 3809

Ala Ile Asn Asn Asn Asn Ile Ile Pro Thr Ala Ile

1225 1230

tatgtccaac gcacctaaaa 3829

<210>38

<211>1233

<212>PRT

<213>谷氨酸棒状杆菌

<400>38

Val Thr Ile Ser Arg Arg Leu Lys Gln Glu Arg Ser Phe Ala Asp Asp

1 5 10 15

Leu Gln Asp Leu Lys Thr Leu Asn Asp Gln Leu Arg Phe Thr Asn Ala

20 25 30

Lys Leu Gln Ala Arg Ile Ser Gly Ile Gly Asn Asp Gly Lys Lys Ile

35 40 45

Thr Arg Pro Thr Pro Leu Leu Ala Leu Asp Phe Gln Leu Thr Val Glu

50 55 60

Glu Tyr Glu Thr Ile Ile Ala Ile Leu Val Glu Ala Val Gly Gly Asn

65 70 75 80

Gln Ser Lys Pro Ala Ile Leu Lys Asp Leu Phe Ile Glu Tyr Pro Leu

85 90 95

Val Phe Leu Ala Ala Leu Ser Gly Thr Ala Met Leu Asp Ala Gln Glu

100 105 110

Gly Phe Trp Pro Ala Phe Trp Lys Arg Thr Gln Val Ser Val Pro Glu

115 120 125

His Val Tyr Asp Ala Ile Arg Lys Glu Leu Val Asn Ser Ile Arg Lys

130 135 140

Asn Gly Leu Glu Thr Phe Ser Leu Ala Asp Leu Asn Arg Arg Glu Tyr

145 150 155 160

Val Gly Leu Ile Gln Leu His Ser Gly Leu Ser Ala Lys Asp Met Leu

165 170 175

Ala Leu Val Lys Phe Ile Asp His Thr Arg Ala Glu Asn Gln Gly Trp

180 185 190

Asp Ser Gly Glu Asp Phe Ala Ser Tyr Ala Lys Ser Val Phe Ser Ser

195 200 205

Gly Asp Asn Leu Leu Thr Thr Glu Ser Leu Lys Gln Leu Val Thr His

210 215 220

Ile Pro Ala Arg Ser Val Asp Phe Ile Ala Arg Val Tyr Glu Leu Thr

225 230 235 240

Asn Trp Tyr Arg Asp Leu Lys Asp Leu Asn Glu Val Glu Ala Phe Val

245 250 255

Gly Thr His Gly Leu Pro Glu Leu Ser Phe Lys Phe Leu Leu Glu Cys

260 265 270

Leu Ser Gly Glu Ala Glu Gln Ile Ala Glu Lys Thr Lys Ala Ala Pro

275 280 285

Ala Ser Leu Glu Asn Leu Glu Pro Pro His Leu Tyr Leu Asp Pro Gln

290 295 300

Ser Phe Glu Leu Ser Leu Val Phe Pro Ala Ile Ser Lys Thr Ala Ala

305 310 315 320

Leu Gln Ile Pro Ala Pro Glu Trp Thr Val Ile Tyr Asp Gly Asn Ser

325 330 335

Ile Lys Val Arg Pro Glu Gln Asp Trp Ser Tyr Gly Gly Phe Ala Glu

340 345 350

Tyr Arg Leu Pro Leu Asp Lys Pro Leu Ser Ser Leu Arg Val Ile Thr

355 360 365

Pro Thr Glu Lys Ser Leu Ile Leu Ile Glu Gly Phe Gly His Lys Asn

370 375 380

Pro Ile Met Phe Phe Lys Asn Asn Gly Gln Pro Tyr Ala Asn Gln Glu

385 390 395 400

Met Leu Ser Gly Asn Ala Val Thr Ala Ile Val Pro Ala Ala Ala Ile

405 410 415

Ile Arg Ala Arg Met Arg Ala Ser Lys Thr Phe Asn Tyr Gln Asp Leu

420 425 430

Gly Pro Leu Ser Gly Trp Asn Lys Trp Val Ile Arg Ser Ile Pro Leu

435 440 445

Lys Arg Ala Glu Ser Ile Thr Val Ser His Gly Gly Phe Arg Lys Glu

450 455 460

Leu Pro Val Arg Arg Lys Val Asp Val Gln Trp Ile Thr Glu Asp Leu

465 470 475 480

Thr Ile Glu Asn Leu Gln Gly Leu Asp His Glu Pro Val Phe His Thr

485 490 495

Ser Pro Arg Ile Glu Phe Pro Thr Ser Gly Ser Asn Trp Val Ile Gln

500 505 510

Tyr Ser Gln Ile Leu Pro Asp Gly Ser Leu Ile Glu Met Glu Asp Tyr

515 520 525

Pro Val Glu Pro Glu Asn Phe Gly Tyr Glu Leu Asp Leu Phe Glu Glu

530 535 540

Ser Asp Asp Pro Trp Val Gly Gln Phe Leu Val Thr Leu Leu Lys Asp

545 550 555 560

Glu Lys Val Tyr Glu Thr Arg Lys Phe Asn Leu Ala Glu Gly Leu Asp

565 570 575

Leu Ser Leu Thr Phe Ser Gly Gly Gly Pro Glu Asn Arg Phe Arg Tyr

580 585 590

Pro Ser Ile Asn Gln Gly Gln Thr Gly Leu Thr Lys Thr Phe Ala Arg

595 600 605

Phe Ser Ser Asn Ser Glu Lys His Ile Arg Phe Pro Asp Glu Ile Ile

610 615 620

Gly Leu Asp Ala Phe Thr Ser Gln Lys Ala Phe Asn Ile Ala Ser Gly

625 630 635 640

Asp Phe Pro Glu Asp Tyr Asn Leu Asp Val Phe Ile Thr Pro Pro Gln

645 650 655

Leu His Tyr Gln Val Pro Val Thr His Ser Gln Thr Lys Trp Glu Ser

660 665 670

Thr Lys Thr Thr Leu Asp Phe Asn Asp Phe Ala Asp Gly Asn Leu Gln

675 680 685

Ile Arg Phe Pro Asn Glu Val Tyr Asp Pro Asn Leu Lys Ile Ile Lys

690 695 700

Met Val Ala Tyr Lys Lys Pro Glu Ser Ser Glu Pro Lys Tyr Leu Ser

705 710 715 720

Lys Ile Gly Ser Ser Lys Val Trp Ser Ile Pro Met Asp Arg Ile Lys

725 730 735

Glu Leu Met Asp Asp Asp Ala Gln Phe Leu Leu Ile Ala Glu Trp Phe

740 745 750

Ala Glu Ser Lys Asp Gln His Arg Glu Lys Ile Ile Ser Glu Ala Lys

755 760 765

Arg Thr Gly Lys Ile Ser Asn Ala Ala Leu Lys Ser Ala Arg Pro Gln

770 775 780

Pro Gln Ala Ser Ser His Ile Ala Thr Ile Glu Lys Lys Pro Leu Leu

785 790 795 800

Ala Ala Ala Glu Ile Lys Leu Ser Thr Val Glu Leu Glu Leu Gly Arg

805 810 815

His Thr Ser Lys Arg Leu Glu Gly Trp Ala Trp Ser Ala Leu Asn Pro

820 825 830

Leu Asp Pro Pro Ile Lys Val Asp Phe Gln Gly Thr Ser Gly Ser Leu

835 840 845

Pro Asp Thr His Phe Val Val Gly Pro Leu Ile Val Glu Val Arg Glu

850 855 860

Lys Glu Phe Leu Ser Gln Trp Gln Pro Lys Val Pro Ser Val Lys Ala

865 870 875 880

Val Val Ala Asn Asp Pro Ser Phe Glu Leu Asp Pro Gln Phe Asp Pro

885 890 895

Phe Leu Thr His Arg Trp Met Phe Ala Pro Arg Ser Gly Lys Val Leu

900 905 910

Leu Pro Gln Glu Ile Arg Thr Val Trp Asp Ala Arg Phe Asn Met Arg

915 920 925

His Val Leu Ala Gln Arg Glu Asn Leu His Val Lys Ser Ile Gln Asp

930 935 940

Phe Asp Asp Ala Thr Ser Thr Tyr Leu Thr Ser Asp Pro Arg Val Ala

945 950 955 960

Leu Asp Glu Leu Asp Lys Ser Ser Ile Pro Ser Asn Ser His Phe Glu

965 970 975

Ser Phe Ile Arg Ser Gly Leu Ala Glu Leu Ser Phe Glu Val Asp Asp

980 985 990

Thr Ala Gly Asp Ile His Arg Val Pro Trp Ile Gly Leu Ile Gln Glu

995 1000 1005

Met Asn Asp Leu Arg Ile Leu Gln Ile Gln Gly Tyr Glu Thr Glu Glu

1010 1015 1020

Arg Ala Ile Glu Arg Arg Asn Ser Gln Ser Tyr Ile Arg Glu Ile Gly

1025 1030 1035 1040

Gly Ser Glu Leu Trp Asn Ile Leu Lys Gly Asn Ser Glu Gly Leu Ser

1045 1050 1055

Leu Ala Gln Lys Cys Ala Pro Gln Ala Thr Glu Ile Asn Val Ile Arg

1060 1065 1070

Asn Ser Gly Leu Glu Ala Met Arg Asn Gly Leu Gly Ala Asp Gln Phe

1075 1080 1085

Ser Ala Glu Phe Ile Ser Ala Asp Ser Arg Leu Arg Ala Gln Leu Glu

1090 1095 1100

Trp Leu Glu Asn Arg Arg Glu Leu Asn Asp Leu Gly Gln Leu Pro Thr

1105 1110 1115 1120

Leu Phe Asp Phe Ala Glu Lys Tyr Glu Tyr Leu Ile Asp His Leu Gly

1125 1130 1135

Asp Asp Arg Ile Lys Val Thr Ala Arg Glu Leu Ser Thr Leu Ala Ser

1140 1145 1150

Glu His Arg Arg Gly Asn Ala Glu Asn Trp Leu Tyr Ala Pro Tyr Val

1155 1160 1165

Ser Phe Ile Tyr Ser Leu Leu Asn Arg Met Ile Ala His Glu Val Ile

1170 1175 1180

Arg Pro Ile Ala Gln Ile Asn Tyr Ser Arg His Asp Trp Ala Asn Ala

1185 1190 1195 1200

Ala Arg Leu Ile Pro Arg Leu Thr Gly Phe Asp Leu Val Ser Ala Glu

1205 1210 1215

Ala Lys Val Leu Ser Ala Ile Asn Asn Asn Asn Ile Ile Pro Thr Ala

1220 1225 1230

Ile

<210>39

<211>1633

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1603)

<223>RXA02376

<400>39

actggtagga tcgaagaacc ttggcgccca cacatccccc tttatttttc tacttatacg 60

tcggcgcccg ttttatcccg atcaggagtg acaccgtttt atg aac cga ttt att 115

Met Asn Arg Phe Ile

1 5

gac cgc gtt gtg cta cac ctc gcc gca ggt gac ggc ggc aat ggt tgt 163

Asp Arg Val Val Leu His Leu Ala Ala Gly Asp Gly Gly Asn Gly Cys

10 15 20

gtc tcg gtg cac cgc gaa aaa ttc aag cca ctt ggt gga cca gac ggc 211

Val Ser Val His Arg Glu Lys Phe Lys Pro Leu Gly Gly Pro Asp Gly

25 30 35

ggc aac ggc ggc cac ggt gga gac atc atc ttg gaa gtc acc gca cag 259

Gly Asn Gly Gly His Gly Gly Asp Ile Ile Leu Glu Val Thr Ala Gln

40 45 50

gtc cac acc ctg ctt gac ttc cac ttc cac cca cac gtg aag gcc gag 307

Val His Thr Leu Leu Asp Phe His Phe His Pro His Val Lys Ala Glu

55 60 65

cgc ggc gct aac ggc gct ggc gat cat cgc aac ggt gcc cga ggc aag 355

Arg Gly Ala Asn Gly Ala Gly Asp His Arg Asn Gly Ala Arg Gly Lys

70 75 80 85

gac ctt gtc ttg gaa gtt cca cca gga act gtc gtg ctt aat gaa aag 403

Asp Leu Val Leu Glu Val Pro Pro Gly Thr Val Val Leu Asn Glu Lys

90 95 100

ggc gag act ctg gca gac ctg acc agc gtg ggc atg aag ttc atc gct 451

Gly Glu Thr Leu Ala Asp Leu Thr Ser Val Gly Met Lys Phe Ile Ala

105 110 115

gct gct ggc ggt aac ggc ggt ttg ggt aac gca gcg ctt gcc tcc aag 499

Ala Ala Gly Gly Asn Gly Gly Leu Gly Asn Ala Ala Leu Ala Ser Lys

120 125 130

gct cgt aag gcc cca ggc ttc gcc ctg atc ggt gag cca ggc gag gcc 547

Ala Arg Lys Ala Pro Gly Phe Ala Leu Ile Gly Glu Pro Gly Glu Ala

135 140 145

cac gac ttg att ctt gaa ctc aaa tcc atg gca gat gtg gga ttg gtg 595

His Asp Leu Ile Leu Glu Leu Lys Ser Met Ala Asp Val Gly Leu Val

150 155 160 165

ggc ttc cca tca gcc ggc aaa tca tca ctg att tct gtg atg tct gca 643

Gly Phe Pro Ser Ala Gly Lys Ser Ser Leu Ile Ser Val Met Ser Ala

170 175 180

gca aag cca aag atc ggt gat tac cca ttc acc acc ctg cag cca aac 691

Ala Lys Pro Lys Ile Gly Asp Tyr Pro Phe Thr Thr Leu Gln Pro Asn

185 190 195

ctc ggc gta gtt aac gtt ggt cat gag act ttc acc atg gca gac gtg 739

Leu Gly Val Val Asn Val Gly His Glu Thr Phe Thr Met Ala Asp Val

200 205 210

cct ggt ttg atc cct ggt gct tct gag ggc aag ggc ttg ggt ctg gat 787

Pro Gly Leu Ile Pro Gly Ala Ser Glu Gly Lys Gly Leu Gly Leu Asp

215 220 225

ttc ttg cgc cac att gag cgc acc tcc gtg ctg gtt cac gtt gtc gat 835

Phe Leu Arg His Ile Glu Arg Thr Ser Val Leu Val His Val Val Asp

230 235 240 245

acc gca acg atg gat cca ggc cgc gat ccg atc tct gat att gag gct 883

Thr Ala Thr Met Asp Pro Gly Arg Asp Pro Ile Ser Asp Ile Glu Ala

250 255 260

ttg gaa gca gaa ctt gcc gcc tac cag tcg gct ttg gat gaa gac acc 931

Leu Glu Ala Glu Leu Ala Ala Tyr Gln Ser Ala Leu Asp Glu Asp Thr

265 270 275

gga ctt ggt gac ttg agc cag cgc cct cgc ctt gtt gtg ttg aac aag 979

Gly Leu Gly Asp Leu Ser Gln Arg Pro Arg Leu Val Val Leu Asn Lys

280 285 290

gct gat gtc cct gag gct gaa gag ctt gct gag ttc ctc aaa gaa gat 1027

Ala Asp Val Pro Glu Ala Glu Glu Leu Ala Glu Phe Leu Lys Glu Asp

295 300 305

att gag aag caa ttc gga tgg ccc gtg ttc att atc tcc gca gtg gca 1075

Ile Glu Lys Gln Phe Gly Trp Pro Val Phe Ile Ile Ser Ala Val Ala

310 315 320 325

cgc aag ggc ttg gat cct ttg aag tac aag ctg ctg gaa atc gtc cag 1123

Arg Lys Gly Leu Asp Pro Leu Lys Tyr Lys Leu Leu Glu Ile Val Gln

330 335 340

gat gcc cga aag aag cgt cca aag gag aag gct gag tct gtc atc att 1171

Asp Ala Arg Lys Lys Arg Pro Lys Glu Lys Ala Glu Ser Val Ile Ile

345 350 355

aag cct aag gct gtt gat cac cgc act aag ggg cag ttc cag atc aag 1219

Lys Pro Lys Ala Val Asp His Arg Thr Lys Gly Gln Phe Gln Ile Lys

360 365 370

cct gac cca gag gtt cag ggc gga ttc atc atc acc ggc gaa aag cca 1267

Pro Asp Pro Glu Val Gln Gly Gly Phe Ile Ile Thr Gly Glu Lys Pro

375 380 385

gag cgc tgg att ttg cag acc gac ttt gaa aac gac gaa gca gtt ggc 1315

Glu Arg Trp Ile Leu Gln Thr Asp Phe Glu Asn Asp Glu Ala Val Gly

390 395 400 405

tac ctg gct gac cgt ctg gcc aag ttg ggc att gag gac ggg ctt cgt 1363

Tyr Leu Ala Asp Arg Leu Ala Lys Leu Gly Ile Glu Asp Gly Leu Arg

410 415 420

aag gca gga gca cat gtg ggt gca aac gtc acc atc gga ggc att tcc 1411

Lys Ala Gly Ala His Val Gly Ala Asn Val Thr Ile Gly Gly Ile Ser

425 430 435

ttc gag tgg gag cca atg acc acc gct ggc gac gat cca gtc ctt acc 1459

Phe Glu Trp Glu Pro Met Thr Thr Ala Gly Asp Asp Pro Val Leu Thr

440 445 450

gga cgt ggc acc gat gtg cgc ctt gaa cag acc tct cgt atc tct gct 1507

Gly Arg Gly Thr Asp Val Arg Leu Glu Gln Thr Ser Arg Ile Ser Ala

455 460 465

gca gag cgt aaa cgc gca tct cag gta cgt cgt ggc ctc atc gat gag 1555

Ala Glu Arg Lys Arg Ala Ser Gln Val Arg Arg Gly Leu Ile Asp Glu

470 475 480 485

ttg gat tat ggc gag gac caa gag gct tcc cgc gaa cgc tgg gaa gga 1603

Leu Asp Tyr Gly Glu Asp Gln Glu Ala Ser Arg Glu Arg Trp Glu Gly

490 495 500

taaaaccgag cacttttcag gtctacgtgt 1633

<210>40

<211>501

<212>PRT

<213>谷氨酸棒状杆菌

<400>40

Met Asn Arg Phe Ile Asp Arg Val Val Leu His Leu Ala Ala Gly Asp

1 5 10 15

Gly Gly Asn Gly Cys Val Ser Val His Arg Glu Lys Phe Lys Pro Leu

20 25 30

Gly Gly Pro Asp Gly Gly Asn Gly Gly His Gly Gly Asp Ile Ile Leu

35 40 45

Glu Val Thr Ala Gln Val His Thr Leu Leu Asp Phe His Phe His Pro

50 55 60

His Val Lys Ala Glu Arg Gly Ala Ash Gly Ala Gly Asp His Arg Asn

65 70 75 80

Gly Ala Arg Gly Lys Asp Leu Val Leu Glu Val Pro Pro Gly Thr Val

85 90 95

Val Leu Asn Glu Lys Gly Glu Thr Leu Ala Asp Leu Thr Ser Val Gly

100 105 110

Met Lys Phe Ile Ala Ala Ala Gly Gly Asn Gly Gly Leu Gly Asn Ala

115 120 125

Ala Leu Ala Ser Lys Ala Arg Lys Ala Pro Gly Phe Ala Leu Ile Gly

130 135 140

Glu Pro Gly Glu Ala His Asp Leu Ile Leu Glu Leu Lys Ser Met Ala

145 150 155 160

Asp Val Gly Leu Val Gly Phe Pro Ser Ala Gly Lys Ser Ser Leu Ile

165 170 175

Ser Val Met Ser Ala Ala Lys Pro Lys Ile Gly Asp Tyr Pro Phe Thr

180 185 190

Thr Leu Gln Pro Asn Leu Gly Val Val Asn Val Gly His Glu Thr Phe

195 200 205

Thr Met Ala Asp Val Pro Gly Leu Ile Pro Gly Ala Ser Glu Gly Lys

210 215 220

Gly Leu Gly Leu Asp Phe Leu Arg His Ile Glu Arg Thr Ser Val Leu

225 230 235 240

Val His Val Val Asp Thr Ala Thr Met Asp Pro Gly Arg Asp Pro Ile

245 250 255

Ser Asp Ile Glu Ala Leu Glu Ala Glu Leu Ala Ala Tyr Gln Ser Ala

260 265 270

Leu Asp Glu Asp Thr Gly Leu Gly Asp Leu Ser Gln Arg Pro Arg Leu

275 280 285

Val Val Leu Asn Lys Ala Asp Val Pro Glu Ala Glu Glu Leu Ala Glu

290 295 300

Phe Leu Lys Glu Asp Ile Glu Lys Gln Phe Gly Trp Pro Val Phe Ile

305 310 315 320

Ile Ser Ala Val Ala Arg Lys Gly Leu Asp Pro Leu Lys Tyr Lys Leu

325 330 335

Leu Glu Ile Val Gln Asp Ala Arg Lys Lys Arg Pro Lys Glu Lys Ala

340 345 350

Glu Ser Val Ile Ile Lys Pro Lys Ala Val Asp His Arg Thr Lys Gly

355 360 365

Gln Phe Gln Ile Lys Pro Asp Pro Glu Val Gln Gly Gly Phe Ile Ile

370 375 380

Thr Gly Glu Lys Pro Glu Arg Trp Ile Leu Gln Thr Asp Phe Glu Asn

385 390 395 400

Asp Glu Ala Val Gly Tyr Leu Ala Asp Arg Leu Ala Lys Leu Gly Ile

405 410 415

Glu Asp Gly Leu Arg Lys Ala Gly Ala His Val Gly Ala Asn Val Thr

420 425 430

Ile Gly Gly Ile Ser Phe Glu Trp Glu Pro Met Thr Thr Ala Gly Asp

435 440 445

Asp Pro Val Leu Thr Gly Arg Gly Thr Asp Val Arg Leu Glu Gln Thr

450 455 460

Ser Arg Ile Ser Ala Ala Glu Arg Lys Arg Ala Ser Gln Val Arg Arg

465 470 475 480

Gly Leu Ile Asp Glu Leu Asp Tyr Gly Glu Asp Gln Glu Ala Ser Arg

485 490 495

Glu Arg Trp Glu Gly

500

<210>41

<211>1144

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1114)

<223>RXA02627

<400>41

tcgcctattg ggggttattt tcgatgcccg atcacagtaa tctcgaacag gttaggtaag 60

gtttgcatac tttattttta tcctgaaggg acgggcgcca atg aaa aag cgt gtc 115

Met Lys Lys Arg Val

1 5

agt tta ttt aag tca gct gca tcg att gtg gtg gtt gcg gga atg gcg 163

Ser Leu Phe Lys Ser Ala Ala Ser Ile Val Val Val Ala Gly Met Ala

10 15 20

ttg ggt atg gtg ggt tgc tca tcg acc gaa tcg gag agt gaa tct agc 211

Leu Gly Met Val Gly Cys Ser Ser Thr Glu Ser Glu Ser Glu Ser Ser

25 30 35

gct gac gtg aca tca tct gcc gat ggg gca tat cca gtg tcc atc gcg 259

Ala Asp Val Thr Ser Ser Ala Asp Gly Ala Tyr Pro Val Ser Ile Ala

40 45 50

acc aaa ttc ggt gat gtc act gtg gaa agc caa cca gaa cgc gtc gtt 307

Thr Lys Phe Gly Asp Val Thr Val Glu Ser Gln Pro Glu Arg Val Val

55 60 65

gcc ctg ggt tgg gga gat gct gag gct gcg ctg gaa ttc ggt gtg cag 355

Ala Leu Gly Trp Gly Asp Ala Glu Ala Ala Leu Glu Phe Gly Val Gln

70 75 80 85

cct gtg ggt gca tca gat tgg ctc gca ttc ggt ggt gaa ggc gtg gga 403

Pro Val Gly Ala Ser Asp Trp Leu Ala Phe Gly Gly Glu Gly Val Gly

90 95 100

ccg tgg att gag gat tct gcc tac gat gaa gcg cca gaa ata atc gga 451

Pro Trp Ile Glu Asp Ser Ala Tyr Asp Glu Ala Pro Glu Ile Ile Gly

105 110 115

acc atg gaa ccg gag tat gaa aag att gca gcg ctt gaa ccg gat ctg 499

Thr Met Glu Pro Glu Tyr Glu Lys Ile Ala Ala Leu Glu Pro Asp Leu

120 125 130

att ttg gac gtg cgc agc tct ggc gac cag gaa cgc tat gac aag ttg 547

Ile Leu Asp Val Arg Ser Ser Gly Asp Gln Glu Arg Tyr Asp Lys Leu

135 140 145

tct tca atc gca ctg acc atc ggc gtt cca gaa ggt ggc gat agc tac 595

Ser Ser Ile Ala Leu Thr Ile Gly Val Pro Glu Gly Gly Asp Ser Tyr

150 155 160 165

ctc acc cca cgc gct gag cag gta acc atg atc gcc act gct ctg ggg 643

Leu Thr Pro Arg Ala Glu Gln Val Thr Met Ile Ala Thr Ala Leu Gly

170 175 180

cag gct gaa cgt ggt gaa gaa gtg aac gct gaa tac gag cag ctc act 691

Gln Ala Glu Arg Gly Glu Glu Val Asn Ala Glu Tyr Glu Gln Leu Thr

185 190 195

gct gat att cgt gca gct cac ccg ggc tgg cct gag aag acc gcg gct 739

Ala Asp Ile Arg Ala Ala His Pro Gly Trp Pro Glu Lys Thr Ala Ala

200 205 210

gct gta tct gca acg gca acc agc tgg ggt gca tac atc aag ggc tcc 787

Ala Val Ser Ala Thr Ala Thr Ser Trp Gly Ala Tyr Ile Lys Gly Ser

215 220 225

aac cgt gta gat act ttg ctg gac ctg ggc ttc cag gaa aac cct gag 835

Asn Arg Val Asp Thr Leu Leu Asp Leu Gly Phe Gln Glu Asn Pro Glu

230 235 240 245

ctg gct aaa cag caa cct ggc gat acg ggt ttc tcc atc aaa ttc agt 883

Leu Ala Lys Gln Gln Pro Gly Asp Thr Gly Phe Ser Ile Lys Phe Ser

250 255 260

gaa gag act ttc ggc gtt gtg gat tcc gac ctg gtt gtc ggc ttt gcc 931

Glu Glu Thr Phe Gly Val Val Asp Ser Asp Leu Val Val Gly Phe Ala

265 270 275

ate ggt atg act cct gag gaa atg gca gag cag gtt cca tgg cag atg 979

Ile Gly Met Thr Pro Glu Glu Met Ala Glu Gln Val Pro Trp Gln Met

280 285 290

ttg acc gcc act cgt gac ggc cgt tcc ttt gtg atg ccc cgt gag att 1027

Leu Thr Ala Thr Arg Asp Gly Arg Ser Phe Val Met Pro Arg Glu Ile

295 300 305

tcc aat gcg ttt tct ttg ggt tcc ccg cag tcc act cgg ttc gcg tta 1075

Ser Asn Ala Phe Ser Leu Gly Ser Pro Gln Ser Thr Arg Phe Ala Leu

310 315 320 325

gac gcc ttg gtg cca ctt ctg gag gag cat gca ggg gag tagtggtccg 1124

Asp Ala Leu Val Pro Leu Leu Glu Glu His Ala Gly Glu

330 335

gtggtgcggg cagggggtag 1144

<210>42

<211>338

<212>PRT

<213>谷氨酸棒状杆菌

<400>42

Met Lys Lys Arg Val Ser Leu Phe Lys Ser Ala Ala Ser Ile Val Val

1 5 10 15

Val Ala Gly Met Ala Leu Gly Met Val Gly Cys Ser Ser Thr Glu Ser

20 25 30

Glu Ser Glu Ser Ser Ala Asp Val Thr Ser Ser Ala Asp Gly Ala Tyr

35 40 45

Pro Val Ser Ile Ala Thr Lys Phe Gly Asp Val Thr Val Glu Ser Gln

50 55 60

Pro Glu Arg Val Val Ala Leu Gly Trp Gly Asp Ala Glu Ala Ala Leu

65 70 75 80

Glu Phe Gly Val Gln Pro Val Gly Ala Ser Asp Trp Leu Ala Phe Gly

85 90 95

Gly Glu Gly Val Gly Pro Trp Ile Glu Asp Ser Ala Tyr Asp Glu Ala

100 105 110

Pro Glu Ile Ile Gly Thr Met Glu Pro Glu Tyr Glu Lys Ile Ala Ala

115 120 125

Leu Glu Pro Asp Leu Ile Leu Asp Val Arg Ser Ser Gly Asp Gln Glu

130 135 140

Arg Tyr Asp Lys Leu Ser Ser Ile Ala Leu Thr Ile Gly Val Pro Glu

145 150 155 160

Gly Gly Asp Ser Tyr Leu Thr Pro Arg Ala Glu Gln Val Thr Met Ile

165 170 175

Ala Thr Ala Leu Gly Gln Ala Glu Arg Gly Glu Glu Val Asn Ala Glu

180 185 190

Tyr Glu Gln Leu Thr Ala Asp Ile Arg Ala Ala His Pro Gly Trp Pro

195 200 205

Glu Lys Thr AlaAla Ala Val Ser Ala Thr Ala Thr Ser Trp Gly Ala

210 215 220

Tyr Ile Lys Gly Ser Asn Arg Val Asp Thr Leu Leu Asp Leu Gly Phe

225 230 235 240

Gln Glu Asn Pro Glu Leu Ala Lys Gln Gln Pro Gly Asp Thr Gly Phe

245 250 255

Ser Ile Lys Phe Ser Glu Glu Thr Phe Gly Val Val Asp Ser Asp Leu

260 265 270

Val Val Gly Phe Ala Ile Gly Met Thr Pro Glu Glu Met Ala Glu Gln

275 280 285

Val Pro Trp Gln Met Leu Thr Ala Thr Arg Asp Gly Arg Ser Phe Val

290 295 300

Met Pro Arg Glu Ile Ser Asn Ala Phe Ser Leu Gly Ser Pro Gln Ser

305 310 315 320

Thr Arg Phe Ala Leu Asp Ala Leu Val Pro Leu Leu Glu Glu His Ala

325 330 335

Gly Glu

<210>43

<211>793

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(763)

<223>RXA02667

<400>43

gttacgttaa atatggtaga attccatagg ttaaagcctg ccccgaattc gattgaaatt 60

tgaggggtag cgcagcaggg tgaagtatta aataggtcgc ttg cat gga caa tgc 115

Leu His Gly Gln Cys

1 5

ggg cgc cac cgg ctg gtt cta gca aaa atg gaa ccg cca tta gaa gga 163

Gly Arg His Arg Leu Val Leu Ala Lys Met Glu Pro Pro Leu Glu Gly

10 15 20

gtg gga atg gaa ttc aag gtc gga gat acc gtc gtt tac ccg cac cac 211

Val Gly Met Glu Phe Lys Val Gly Asp Thr Val Val Tyr Pro His His

25 30 35

gga gct gca att att tca gcc ctg gag cag cgt gaa atg aat ggt gag 259

Gly Ala Ala Ile Ile Ser Ala Leu Glu Gln Arg Glu Met Asn Gly Glu

40 45 50

acg gtg gac tac ctg gtt ctc cag atc aat cat tcc gat ctc gtc gtt 307

Thr Val Asp Tyr Leu Val Leu Gln Ile Asn His Ser Asp Leu Val Val

55 60 65

cgc gtt cca gca aag aac gct gaa ctc gtt ggc gtg cgt gac gtt gtc 355

Arg Val Pro Ala Lys Asn Ala Glu Leu Val Gly Val Arg Asp Val Val

70 75 80 85

ggc gag gag ggc ctg cag aag gtt ttc tct gtt ctt cgt gaa att gac 403

Gly Glu Glu Gly Leu Gln Lys Val Phe Ser Val Leu Arg Glu Ile Asp

90 95 100

gtc gaa gaa gcc ggc aac tgg tcc cgc cgt tac aag gct aac cag gag 451

Val Glu Glu Ala Gly Asn Trp Ser Arg Arg Tyr Lys Ala Asn Gln Glu

105 110 115

cgt ttg gct tcc ggt gac gtg aac aag gtc gct gag gtt gtc cgt gac 499

Arg Leu Ala Ser Gly Asp Val Asn Lys Val Ala Glu Val Val Arg Asp

120 125 130

ctg tgg cgt cgt gat cag gat cgt ggc ctt tcc gct ggt gag aag cgc 547

Leu Trp Arg Arg Asp Gln Asp Arg Gly Leu Ser Ala Gly Glu Lys Arg

135 140 145

atg ctc tcc aag gcg cgt cag gtt ctt gtt ggt gag ctc gcg ctc gcc 595

Met Leu Ser Lys Ala Arg Gln Val Leu Val Gly Glu Leu Ala Leu Ala

150 155 160 165

gaa acc gtg gac gat gag aag gcg gat gct ttc ctc agc cag gtc gat 643

Glu Thr Val Asp Asp Glu Lys Ala Asp Ala Phe Leu Ser Gln Val Asp

170 175 180

gag acc att gct cgc cac cgc gct gac ctg ctc ggc gac gag gaa gag 691

Glu Thr Ile Ala Arg His Arg Ala Asp Leu Leu Gly Asp Glu Glu Glu

185 190 195

aag aag gac gca ttc gac gac ttc gac gat tcc gac gtg gat ctt gac 739

Lys Lys Asp Ala Phe Asp Asp Phe Asp Asp Ser Asp Val Asp Leu Asp

200 205 210

gat ctg agc ttc gac gac gaa gat tagacgccca tgtcgtctac acgaatcccc 793

Asp Leu Ser Phe Asp Asp Glu Asp

215 220

<210>44

<211>221

<212>PRT

<213>谷氨酸棒状杆菌

<400>44

Leu His Gly Gln Cys Gly Arg His Arg Leu Val Leu Ala Lys Met Glu

1 5 10 15

Pro Pro Leu Glu Gly Val Gly Met Glu Phe Lys Val Gly Asp Thr Val

20 25 30

Val Tyr Pro His His Gly Ala Ala Ile Ile Ser Ala Leu Glu Gln Arg

35 40 45

Glu Met Asn Gly Glu Thr Val Asp Tyr Leu Val Leu Gln Ile Asn His

50 55 60

Ser Asp Leu Val Val Arg Val Pro Ala Lys Asn Ala Glu Leu Val Gly

65 70 75 80

Val Arg Asp Val Val Gly Glu Glu Gly Leu Gln Lys Val Phe Ser Val

85 90 95

Leu Arg Glu Ile Asp Val Glu Glu Ala Gly Asn Trp Ser Arg Arg Tyr

100 105 110

Lys Ala Asn Gln Glu Arg Leu Ala Ser Gly Asp Val Asn Lys Val Ala

115 120 125

Glu Val Val Arg Asp Leu Trp Arg Arg Asp Gln Asp Arg Gly Leu Ser

130 135 140

Ala Gly Glu Lys Arg Met Leu Ser Lys Ala Arg Gln Val Leu Val Gly

145 150 155 160

Glu Leu Ala Leu Ala Glu Thr Val Asp Asp Glu Lys Ala Asp Ala Phe

165 170 175

Leu Ser Gln Val Asp Glu Thr Ile Ala Arg His Arg Ala Asp Leu Leu

180 185 190

Gly Asp Glu Glu Glu Lys Lys Asp Ala Phe Asp Asp Phe Asp Asp Ser

195 200 205

Asp Val Asp Leu Asp Asp Leu Ser Phe Asp Asp Glu Asp

210 215 220

<210>45

<211>1468

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1438)

<223>RXA02758

<400>45

ggctggtgct tttcatattc cgacttgggg cacccctgaa tacatctcac ccaattcccc 60

ataactagac aattgcccag caacgactga taagtctcca atg tcg tgt tcc gcg 115

Met Ser Cys Ser Ala

1 5

ctc aga cat gag aca att gtt gcc gtg act gaa ctc atc cag aat gaa 163

Leu Arg His Glu Thr Ile Val Ala Val Thr Glu Leu Ile Gln Asn Glu

10 15 20

tcc caa gaa atc gct gag ctg gaa gcc ggc cag cag gtt gca ttg cgt 211

Ser Gln Glu Ile Ala Glu Leu Glu Ala Gly Gln Gln Val Ala Leu Arg

25 30 35

gaa ggt tat ctt cct gcg gtg atc aca gtg agc ggt aaa gac cgc cca 259

Glu Gly Tyr Leu Pro Ala Val Ile Thr Val Ser Gly Lys Asp Arg Pro

40 45 50

ggt gtg act gcc gcg ttc ttt agg gtc ttg tcc gct aat cag gtt cag 307

Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala Asn Gln Val Gln

55 60 65

gtc ttg gac gtt gag cag tca atg ttc cgt ggc ttt ttg aac ttg gcg 355

Val Leu Asp Val Glu Gln Ser Met Phe Arg Gly Phe Leu Asn Leu Ala

70 75 80 85

gcg ttt gtg ggt atc gca cct gag cgt gtc gag acc gtc acc aca ggc 403

Ala Phe Val Gly Ile Ala Pro Glu Arg Val Glu Thr Val Thr Thr Gly

90 95 100

ctg act gac acc ctc aag gtg cat gga cag tcc gtg gtg gtg gag ctg 451

Leu Thr Asp Thr Leu Lys Val His Gly Gln Ser Val Val Val Glu Leu

105 110 115

cag gaa act gtg cag tcg tcc cgt cct cgt tct tcc cat gtt gtt gtg 499

Gln Glu Thr Val Gln Ser Ser Arg Pro Arg Ser Ser His Val Val Val

120 125 130

gtg ttg ggt gat ccg gtt gat gcg ttg gat att tcc cgc att ggt cag 547

Val Leu Gly Asp Pro Val Asp Ala Leu Asp Ile Ser Arg Ile Gly Gln

135 140 145

acc ctg gcg gat tac gat gcc aac att gac acc att cgt ggt att tcg 595

Thr Leu Ala Asp Tyr Asp Ala Asn Ile Asp Thr Ile Arg Gly Ile Ser

150 155 160 165

gat tac cct gtg acc ggc ctg gag ctg aag gtg act gtg ccg gat gtc 643

Asp Tyr Pro Val Thr Gly Leu Glu Leu Lys Val Thr Val Pro Asp Val

170 175 180

agc cct ggt ggt ggt gaa gcg atg cgt aag gcg ctt gct gct ctt acc 691

Ser Pro Gly Gly Gly Glu Ala Met Arg Lys Ala Leu Ala Ala Leu Thr

185 190 195

tct gag ctg aat gtg gat att gcg att gag cgt tct ggt ttg ctg cgt 739

Ser Glu Leu Asn Val Asp Ile Ala Ile Glu Arg Ser Gly Leu Leu Arg

200 205 210

cgt tct aag cgt ctg gtg tgc ttc gat tgt gat tcc acg ttg atc act 787

Arg Ser Lys Arg Leu Val Cys Phe Asp Cys Asp Ser Thr Leu Ile Thr

215 220 225

ggt gag gtc att gag atg ctg gcg gct cac gcg ggc aag gaa gct gaa 835

Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly Lys Glu Ala Glu

230 235 240 246

gtt gcg gca gtt act gag cgt gcg atg cgc ggt gag ctc gat ttc gag 883

Val Ala Ala Val Thr Glu Arg Ala Met Arg Gly Glu Leu Asp Phe Glu

250 255 260

gag tct ctg cgt gag cgt gtg aag gcg ttg gct ggt ttg gat gcg tcg 931

Glu Ser Leu Arg Glu Arg Val Lys Ala Leu Ala Gly Leu Asp Ala Ser

265 270 275

gtg atc gat gag gtc gct gcc gct att gag ctg acc cct ggt gcg cgc 979

Val Ile Asp Glu Val Ala Ala Ala Ile Glu Leu Thr Pro Gly Ala Arg

280 285 290

acc acg atc cgt acg ctg aac cgc atg ggt tac cag acc gct gtt gtt 1027

Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr Gln Thr Ala Val Val

295 300 305

tcc ggt ggt ttc atc cag gtg ttg gaa ggt ttg gct gag gag ttg gag 1075

Ser Gly Gly Phe Ile Gln Val Leu Glu Gly Leu Ala Glu Glu Leu Glu

310 315 320 325

ttg gat tat gtc cgc gcc aac act ttg gaa atc gtt gat ggc aag ctg 1123

Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val Asp Gly Lys Leu

330 335 340

acc ggc aac gtc acc gga aag atc gtt gac cgc gct gcg aag gct gag 1171

Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg Ala Ala Lys Ala Glu

345 350 355

ttc ctc cgt gag ttc gct gcg gat tct ggc ctg aag atg tac cag act 1219

Phe Leu Arg Glu Phe Ala Ala Asp Ser Gly Leu Lys Met Tyr Gln Thr

360 365 370

gtc gct gtc ggt gat ggc gct aat gac atc gat atg ctc tcc gct gcg 1267

Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met Leu Ser Ala Ala

375 380 385

ggt ctg ggt gtt gct ttc aac gcg aag cct gcg ctg aag gag att gcg 1315

Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala Leu Lys Glu Ile Ala

390 395 400 405

gat act tcc gtg aac cac cca ttc ctc gac gag gtt ttg cac atc atg 1363

Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val Leu His Ile Met

410 415 420

ggc att tcc cgc gac gag atc gat ctg gcg gat cag gaa gac ggc act 1411

Gly Ile Ser Arg Asp Glu Ile Asp Leu Ala Asp Gln Glu Asp Gly Thr

425 430 435

ttc cac cgc gtt cca ttg acc aat gcc taaagattcg cttctcgacg 1458

Phe His Arg Val Pro Leu Thr Asn Ala

440 445

cccacctcct 1468

<210>46

<211>446

<212>PRT

<213>谷氨酸棒状杆菌

<400>46

Met Ser Cys Ser Ala Leu Arg His Glu Thr Ile Val Ala Val Thr Glu

1 5 10 15

Leu Ile Gln Asn Glu Ser Gln Glu Ile Ala Glu Leu Glu Ala Gly Gln

20 25 30

Gln Val Ala Leu Arg Glu Gly Tyr Leu Pro Ala Val Ile Thr Val Ser

35 40 45

Gly Lys Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser

50 55 60

Ala Asn Gln Val Gln Val Leu Asp Val Glu Gln Ser Met Phe Arg Gly

65 70 75 80

Phe Leu Asn Leu Ala Ala Phe Val Gly Ile Ala Pro Glu Arg Val Glu

85 90 95

Thr Val Thr Thr Gly Leu Thr Asp Thr Leu Lys Val His Gly Gln Ser

100 105 110

Val Val Val Glu Leu Gln Glu Thr Val Gln Ser Ser Arg Pro Arg Ser

115 120 125

Ser His Val Val Val Val Leu Gly Asp Pro Val Asp Ala Leu Asp Ile

130 135 140

Ser Arg Ile Gly Gln Thr Leu Ala Asp Tyr Asp Ala Asn Ile Asp Thr

145 150 155 160

Ile Arg Gly Ile Ser Asp Tyr Pro Val Thr Gly Leu Glu Leu Lys Val

165 170 175

Thr Val Pro Asp Val Ser Pro Gly Gly Gly Glu Ala Met Arg Lys Ala

180 185 190

Leu Ala Ala Leu Thr Ser Glu Leu Asn Val Asp Ile Ala Ile Glu Arg

195 200 205

Ser Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Cys Phe Asp Cys Asp

210 215 220

Ser Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala

225 230 235 240

Gly Lys Glu Ala Glu Val Ala Ala Val Thr Glu Arg Ala Met Arg Gly

245 250 255

Glu Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Lys Ala Leu Ala

260 265 270

Gly Leu Asp Ala Ser Val Ile Asp Glu Val Ala Ala Ala Ile Glu Leu

275 280 285

Thr Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr

290 295 300

Gln Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Gly Leu

305 310 315 320

Ala Glu Glu Leu Glu Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile

325 330 335

Val Asp Gly Lys Leu Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg

340 345 350

Ala Ala Lys Ala Glu Phe Leu Arg Glu Phe Ala Ala Asp Ser Gly Leu

355 360 365

Lys Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp

370 375 380

Met Leu Ser Ala Ala Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala

385 390 395 400

Leu Lys Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu

405 410 415

Val Leu His Ile Met Gly Ile Ser Arg Asp Glu Ile Asp Leu Ala Asp

420 425 430

Gln Glu Asp Gly Thr Phe His Arg Val Pro Leu Thr Asn Ala

435 440 445

<210>47

<211>844

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(814)

<223>RXA02910

<400>47

tagcggacca acctaggccg atacccatgt ggaaatctcg acgtcttaaa tggacgattg 60

gagctaaaac cacgaacagc tgggattttc cacgatagga ttg ggt ctc gtg gag 115

Leu Gly Leu Val Glu

1 5

att cgt tgg ttg gaa ggc ttt atc gcg gtc gcg gaa gaa ttg cac ttt 163

Ile Arg Trp Leu Glu Gly Phe Ile Ala Val Ala Glu Glu Leu His Phe

10 15 20

agt aat gct gcg att cgt ttg ggg atg ccg caa tcg ccg ttg agt cag 211

Ser Asn Ala Ala Ile Arg Leu Gly Met Pro Gln Ser Pro Leu Ser Gln

25 30 35

ttg atc cgg cgg ttg gag tcg gag ttg ggg cag aag ctt ttt gat cgc 259

Leu Ile Arg Arg Leu Glu Ser Glu Leu Gly Gln Lys Leu Phe Asp Arg

40 45 50

agt acc cgg tcg gtg gag tta act gcc gcg ggt cgg gcg ttt ttg cca 307

Ser Thr Arg Ser Val Glu Leu Thr Ala Ala Gly Arg Ala Phe Leu Pro

55 60 65

cat gcc agg ggg att gtg gcg agc gct gcg gtg gcg agg gaa gct gtg 355

His Ala Arg Gly Ile Val Ala Ser Ala Ala Val Ala Arg Glu Ala Val

70 75 80 85

aat gct gcc gag ggg gag atc gtt ggt gtt gtt cgc att ggt ttt tct 403

Asn Ala Ala Glu Gly Glu Ile Val Gly Val Val Arg Ile Gly Phe Ser

90 95 100

ggt gtg ctg aac tat tcc acg ctg ccg ctt ttg acc agt gag gtg cat 451

Gly Val Leu Asn Tyr Ser Thr Leu Pro Leu Leu Thr Ser Glu Val His

105 110 115

aaa cgg ctt cct aat gtg gag ttg gag ctc gtt ggt cag aag ttg acg 499

Lys Arg Leu Pro Asn Val Glu Leu Glu Leu Val Gly Gln Lys Leu Thr

120 125 130

agg gaa gcg gta agt ttg ctg cgc ttg ggg gcg ttg gat att acg ttg 547

Arg Glu Ala Val Ser Leu Leu Arg Leu Gly Ala Leu Asp Ile Thr Leu

135 140 145

atg ggt ttg ccc att gag gat cca gag att gag act cgg ctg att agt 595

Met Gly Leu Pro Ile Glu Asp Pro Glu Ile Glu Thr Arg Leu Ile Ser

150 155 160 165

ttg gaa gag ttt tgc gtg gtg ttg ccg aag gat cat cgt ctt gcg ggg 643

Leu Glu Glu Phe Cys Val Val Leu Pro Lys Asp His Arg Leu Ala Gly

170 175 180

gaa gga gtg gtg gat ttg gtg gat ctg gct aaa gat ggg ttt gtg acg 691

Glu Gly Val Val Asp Leu Val Asp Leu Ala Lys Asp Gly Phe Val Thr

185 190 195

acg ccg gag ttt gcg ggg tct gtg ttt agg aat tcc acc ttt cag ttg 739

Thr Pro Glu Phe Ala Gly Ser Val Phe Arg Asn Ser Thr Phe Gln Leu

200 205 210

tgt gct gag gct ggt ttt gtg ccg agg atc agc cag caa gtt aat gat 787

Cys Ala Glu Ala Gly Phe Val Pro Arg Ile Ser Gln Gln Val Asn Asp

215 220 225

cct tac atg gcg ctg ttg ttg gcg cgg tagtcaatca tgggggagta 834

Pro Tyr Met Ala Leu Leu Leu Ala Arg

230 235

tcccaccgta 844

<210>48

<211>238

<212>PRT

<213>谷氨酸棒状杆菌

<400>48

Leu Gly Leu Val Glu Ile Arg Trp Leu Glu Gly Phe Ile Ala Val Ala

1 5 10 15

Glu Glu Leu His Phe Ser Asn Ala Ala Ile Arg Leu Gly Met Pro Gln

20 25 30

Ser Pro Leu Ser Gln Leu Ile Arg Arg Leu Glu Ser Glu Leu Gly Gln

35 40 45

Lys Leu Phe Asp Arg Ser Thr Arg Ser Val Glu Leu Thr Ala Ala Gly

50 55 60

Arg Ala Phe Leu Pro His Ala Arg Gly Ile Val Ala Ser Ala Ala Val

65 70 75 80

Ala Arg Glu Ala Val Asn Ala Ala Glu Gly Glu Ile Val Gly Val Val

85 90 95

Arg Ile Gly Phe Ser Gly Val Leu Asn Tyr Ser Thr Leu Pro Leu Leu

100 105 110

Thr Ser Glu Val His Lys Arg Leu Pro Asn Val Glu Leu Glu Leu Val

115 120 125

Gly Gln Lys Leu Thr Arg Glu Ala Val Ser Leu Leu Arg Leu Gly Ala

130 135 140

Leu Asp Ile Thr Leu Met Gly Leu Pro Ile Glu Asp Pro Glu Ile Glu

145 150 155 160

Thr Arg Leu Ile Ser Leu Glu Glu Phe Cys Val Val Leu Pro Lys Asp

165 170 175

His Arg Leu Ala Gly Glu Gly Val Val Asp Leu Val Asp Leu Ala Lys

180 185 190

Asp Gly Phe Val Thr Thr Pro Glu Phe Ala Gly Ser Val Phe Arg Asn

195 200 205

Ser Thr Phe Gln Leu Cys Ala Glu Ala Gly Phe Val Pro Arg Ile Ser

210 215 220

Gln Gln Val Asn Asp Pro Tyr Met Ala Leu Leu Leu Ala Arg

225 230 235

<210>49

<211>1348

<212>DNA

<21 3>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1318)

<223>RXA03100

<400>49

aaactctaaa aactatagag ctatagaaac tttaacttcg gaggtattca tgtcccgtcc 60

aatcgttaaa caagcattca ccgtcaccgc agtcaccgcg atg gct ttt gcc ctg 115

Met Ala Phe Ala Leu

1 5

gca tca tgc acc cgc gca gtg gat gca acc tcc gca gat gga acc gcg 163

Ala Ser Cys Thr Arg Ala Val Asp Ala Thr Ser Ala Asp Gly Thr Ala

10 15 20

agc aac acc gca gct tcc tgt gtg gat aca tcc ggc gac tcc atc aaa 211

Ser Asn Thr Ala Ala Ser Cys Val Asp Thr Ser Gly Asp Ser Ile Lys

25 30 35

atc ggc ttc atc aac tcc ctg tcc ggc acg atg gct atc tct gaa acc 259

Ile Gly Phe Ile Asn Ser Leu Ser Gly Thr Met Ala Ile Ser Glu Thr

40 45 50

acg gtg aac caa tcc ctg cac atg gca gcc gat gaa atc aac gca gcc 307

Thr Val Asn Gln Ser Leu His MetAla Ala Asp Glu Ile Asn Ala Ala

55 60 65

ggc ggc gtt ttg ggc aag cag ttg gag atc tct gaa gaa gac ggc gcc 355

Gly Gly Val Leu Gly Lys Gln Leu Glu Ile Ser Glu Glu Asp Gly Ala

70 75 80 85

agc gaa ccc gcc acc ttc gcc gaa cgc tcc caa cgc ctc atc cag cag 403

Ser Glu Pro Ala Thr Phe Ala Glu Arg Ser Gln Arg Leu Ile Gln Gln

90 95 100

gaa tgc gtt gca gcc gtg ttt ggt gga tgg acc tcc gcc tcc cgc aaa 451

Glu Cys Val Ala Ala Val Phe Gly Gly Trp Thr Ser Ala Ser Arg Lys

105 110 115

gca atg ctc ccc gtc ttt gag ggc aat aac tcc ctg ctg ttc tac ccg 499

Ala Met Leu Pro Val Phe Glu Gly Asn Asn Ser Leu Leu Phe Tyr Pro

120 125 130

gtg cag tac gag ggc atg gaa tcc tcg ccg aat att ttc tac acc ggc 547

Val Gln Tyr Glu Gly Met Glu Ser Ser Pro Asn Ile Phe Tyr Thr Gly

135 140 145

gcc acc acc aac cag cag atc atc ccg gct ctt gat tac ctg cgt gaa 595

Ala Thr Thr Asn Gln Gln Ile Ile Pro Ala Leu Asp Tyr Leu Arg Glu

150 155 160 165

aac ggc ctg aac cgc ctt ttc ctt gtc ggt tcc gat tat gtt ttc cca 643

Asn Gly Leu Asn Arg Leu Phe Leu Val Gly Ser Asp Tyr Val Phe Pro

170 175 180

cgc act gca aat tcc atc atc aag gac tac gcc gaa gcc aat ggt atg 691

Arg Thr Ala Asn Ser Ile Ile Lys Asp Tyr Ala Glu Ala Asn Gly Met

185 190 195

gaa atc gtc ggc gaa gac tac gcg ccg ttg gga tcc acc gac ttc acc 739

Glu Ile Val Gly Glu Asp Tyr Ala Pro Leu Gly Ser Thr Asp Phe Thr

200 205 210

acc atc gcc aac cgc atg cgt gac tcc aac gca gat gcc gtg ttc aac 787

Thr Ile Ala Asn Arg Met Arg Asp Ser Asn Ala Asp Ala Val Phe Asn

215 220 225

act ttg aat ggc gat tcc aac gtg gcg ttc ttc cgc cag tac aac agc 835

Thr Leu Asn Gly Asp Ser Asn Val Ala Phe Phe Arg Gln Tyr Asn Ser

230 235 240 245

ctc ggc ttc aat gca gac acc ctt ccg gtg atg tca gta tcc att gcg 883

Leu Gly Phe Asn Ala Asp Thr Leu Pro Val Met Ser Val Ser Ile Ala

250 255 260

gaa gaa gaa gtc gga ggc atc ggc acc gca aat att gag ggc cag ctg 931

Glu Glu Glu Val Gly Gly Ile Gly Thr Ala Asn Ile Glu Gly Gln Leu

265 270 275

gtg gcg tgg gac tac tac caa acc atc gac acc cca gaa aac gag acc 979

Val Ala Trp Asp Tyr Tyr Gln Thr Ile Asp Thr Pro Glu Asn Glu Thr

280 285 290

ttc gtg gag aat ttc aag gac ctc tac ggc cag gac aaa gtg acc tcc 1027

Phe Val Glu Asn Phe Lys Asp Leu Tyr Gly Gln Asp Lys Val Thr Ser

295 300 305

gat ccg atg gaa gct gct tac act agc ctc tac ctc tgg aaa gaa atg 1075

Asp Pro Met Glu Ala Ala Tyr Thr Ser Leu Tyr Leu Trp Lys Glu Met

310 315 320 325

gta gag aag gcc gat tcc ttt gat gtc gcc gca att caa gca gcc gcc 1123

Val Glu Lys Ala Asp Ser Phe Asp Val Ala Ala Ile Gln Ala Ala Ala

330 335 340

gac gga acc act ttt gat gca cca gaa gga acc gtg gtg gtt gac ggc 1171

Asp Gly Thr Thr Phe Asp Ala Pro Glu Gly Thr Val Val Val Asp Gly

345 350 355

gat aac cac cac atc tcc aaa aca ccg cgc atc ggt cga atc cgc ccg 1219

Asp Asn His His Ile Ser Lys Thr Pro Arg Ile Gly Arg Ile Arg Pro

360 365 370

gat gga ttg atc gac acc att tgg gaa acc gat tcc cca gtt gat ccg 1267

Asp Gly Leu Ile Asp Thr Ile Trp Glu Thr Asp Ser Pro Val Asp Pro

375 380 385

gac cca tac ttg tct tcc tat gac tgg gcc aag acc acc gct gcg act 1315

Asp Pro Tyr Leu Ser Ser Tyr Asp Trp Ala Lys Thr Thr Ala Ala Thr

390 395 400 405

tcc taagagataa aaatcatgga cattttactc 1348

Ser

<210>50

<211>406

<212>PRT

<213>谷氨酸棒状杆菌

<400>50

Met Ala Phe Ala Leu Ala Ser Cys Thr Arg Ala Val Asp Ala Thr Ser

1 5 10 15

Ala Asp Gly Thr Ala Ser Asn Thr Ala Ala Ser Cys Val Asp Thr Ser

20 25 30

Gly Asp Ser Ile Lys Ile Gly Phe Ile Asn Ser Leu Ser Gly Thr Met

35 40 45

Ala Ile Ser Glu Thr Thr Val Asn Gln Ser Leu His Met Ala Ala Asp

50 55 60

Glu Ile Asn Ala Ala Gly Gly Val Leu Gly Lys Gln Leu Glu Ile Ser

65 70 75 80

Glu Glu Asp Gly Ala Ser Glu Pro Ala Thr Phe Ala Glu Arg Ser Gln

85 90 95

Arg Leu Ile Gln Gln Glu Cys Val Ala Ala Val Phe Gly Gly Trp Thr

100 105 110

Ser Ala Ser Arg Lys Ala Met Leu Pro Val Phe Glu Gly Asn Asn Ser

115 120 125

Leu Leu Phe Tyr Pro Val Gln Tyr Glu Gly Met Glu Ser Ser Pro Asn

130 135 140

Ile Phe Tyr Thr Gly Ala Thr Thr Asn Gln Gln Ile Ile Pro Ala Leu

145 150 155 160

Asp Tyr Leu Arg Glu Asn Gly Leu Asn Arg Leu Phe Leu Val Gly Ser

165 170 175

Asp Tyr Val Phe Pro Arg Thr Ala Asn Ser Ile Ile Lys Asp Tyr Ala

180 185 190

Glu Ala Asn Gly Met Glu Ile Val Gly Glu Asp Tyr Ala Pro Leu Gly

195 200 205

Ser Thr Asp Phe Thr Thr Ile Ala Asn Arg Met Arg Asp Ser Asn Ala

210 215 220

Asp Ala Val Phe Asn Thr Leu Asn Gly Asp Ser Asn Val Ala Phe Phe

225 230 235 240

Arg Gln Tyr Asn Ser Leu Gly Phe Asn Ala Asp Thr Leu Pro Val Met

245 250 255

Ser Val Ser Ile Ala Glu Glu Glu Val Gly Gly Ile Gly Thr Ala Asn

260 265 270

Ile Glu Gly Gln Leu Val Ala Trp Asp Tyr Tyr Gln Thr Ile Asp Thr

275 280 285

Pro Glu Asn Glu Thr Phe Val Glu Asn Phe Lys Asp Leu Tyr Gly Gln

290 295 300

Asp Lys Val Thr Ser Asp Pro Met Glu Ala Ala Tyr Thr Ser Leu Tyr

305 310 315 320

Leu Trp Lys Glu Met Val Glu Lys Ala Asp Ser Phe Asp Val Ala Ala

325 330 335

Ile Gln Ala Ala Ala Asp Gly Thr Thr Phe Asp Ala Pro Glu Gly Thr

340 345 350

Val Val Val Asp Gly Asp Asn His His Ile Ser Lys Thr Pro Arg Ile

355 360 365

Gly Arg Ile Arg Pro Asp Gly Leu Ile Asp Thr Ile Trp Glu Thr Asp

370 375 380

Ser Pro Val Asp Pro Asp Pro Tyr Leu Ser Set Tyr Asp Trp Ala Lys

385 390 395 400

Thr Thr Ala Ala Thr Ser

405

<210>51

<211>850

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(820)

<223>RXA03 127

<400>51

aggtagaaca tctgaaagca cttcagataa gacggatggg gtctgatgga aacgacagtc 60

gatatgatca gaaccatctc cagattagga agtgaacaca atg gaa agc tcc aaa 115

Met Glu Ser Ser Lys

1 5

aag act tcg cga tca agg tcc act act caa gaa gca gtg cgc gac att 163

Lys Thr Ser Arg Ser Arg Ser Thr Thr Gln Glu Ala Val Arg Asp Ile

10 15 20

aaa aaa tac att cgg gac aac cgg ctg cgt acg gga gac ctt ctt cct 211

Lys Lys Tyr Ile Arg Asp Asn Arg Leu Arg Thr Gly Asp Leu Leu Pro

25 30 35

tcc gaa gcg ttc tta tgt gag gaa ttg ggt tgt tcc cgt tct gcg atc 259

Ser Glu Ala Phe Leu Cys Glu Glu Leu Gly Cys Ser Arg Ser Ala Ile

40 45 50

agg gag gcg atc cgc gcg ctc gtg acc ttg gac atc gtc gag gtt cgc 307

Arg Glu Ala Ile Arg Ala Leu Val Thr Leu Asp Ile Val Glu Val Arg

55 60 65

cac ggc tac ggc act ttc gtg tcc agg atg tcc ctc gag ccc ctg atc 355

His Gly Tyr Gly Thr Phe Val Ser Arg Met Ser Leu Glu Pro Leu Ile

70 75 80 85

aac ggg atg gtg ttc cgc acg gtg ttg gac aat gac acc tcg gtg gaa 403

Asn Gly Met Val Phe Arg Thr Val Leu Asp Asn Asp Thr Ser Val Glu

90 95 100

aac ctt ttc tac gtg gtg gat acc cgc gaa atc ctt gac ctt tca ctt 451

Asn Leu Phe Tyr Val Val Asp Thr Arg Glu Ile Leu Asp Leu Ser Leu

105 110 115

ggc gaa gag ctg atc gag gtg ttc acc gac gat gac cgc gag cta ctc 499

Gly Glu Glu Leu Ile Glu Val Phe Thr Asp Asp Asp Arg Glu Leu Leu

120 125 130

ctt gat ctg gtg gac aag atg cgc gag cac aac gat cag ggc gaa tcc 547

Leu Asp Leu Val Asp Lys Met Arg Glu His Asn Asp Gln Gly Glu Ser

135 140 145

ttt gtg gtg gag gat caa aaa ttc cac cga gca ctc cta gcg cga acg 595

Phe Val Val Glu Asp Gln Lys Phe His Arg Ala Leu Leu Ala Arg Thr

150 155 160 165

aaa aac ccg ctg att aga gag ctc aac gat gcg ttt tgg cag atc caa 643

Lys Asn Pro Leu Ile Arg Glu Leu Asn Asp Ala Phe Trp Gln Ile Gln

170 175 180

acc gag gcg cag ccc atg ctc aat ctg gct atg ccc gca gac atc gac 691

Thr Glu Ala Gln Pro Met Leu Asn Leu Ala Met Pro Ala Asp Ile Asp

185 190 195

gaa acc atc aaa gct cac agc gac atc gtc gaa gcg ctc tcc agc ggg 739

Glu Thr Ile Lys Ala His Ser Asp Ile Val Glu Ala Leu Ser Ser Gly

200 205 210

aac atc gac gat tat cgc agc gcc gtg ctc gct cac tac gcg ccg ttt 787

Asn Ile Asp Asp Tyr Arg Ser Ala Val Leu Ala His Tyr Ala Pro Phe

215 220 225

cgc cgc atg att tcc aac atg ctc gat gcg cac tagcctcatt gcgcgcgggt840

Arg Arg Met Ile Ser Asn Met Leu Asp Ala His

230 235 240

tgtaccgcat 850

<210>52

<211>240

<212>PRT

<213>谷氨酸棒状杆菌

<400>52

Met Glu Ser Ser Lys Lys Thr Ser Arg Ser Arg Ser Thr Thr Gln Glu

1 5 10 15

Ala Val Arg Asp Ile Lys Lys Tyr Ile Arg Asp Asn Arg Leu Arg Thr

20 25 30

Gly Asp Leu Leu Pro Ser Glu Ala Phe Leu Cys Glu Glu Leu Gly Cys

35 40 45

Ser Arg Ser Ala Ile Arg Glu Ala Ile Arg Ala Leu Val Thr Leu Asp

50 55 60

Ile Val Glu Val Arg His Gly Tyr Gly Thr Phe Val Ser Arg Met Ser

65 70 75 80

Leu Glu Pro Leu Ile Asn Gly Met Val Phe Arg Thr Val Leu Asp Asn

85 90 95

Asp Thr Ser Val Glu Asn Leu Phe Tyr Val Val Asp Thr Arg Glu Ile

100 105 110

Leu Asp Leu Ser Leu Gly Glu Glu Leu Ile Glu Val Phe Thr Asp Asp

115 120 125

Asp Arg Glu Leu Leu Leu Asp Leu Val Asp Lys Met Arg Glu His Asn

130 135 140

Asp Gln Gly Glu Ser Phe Val Val Glu Asp Gln Lys Phe His Arg Ala

145 150 155 160

Leu Leu Ala Arg Thr Lys Asn Pro Leu Ile Arg Glu Leu Asn Asp Ala

165 170 175

Phe Trp Gln Ile Gln Thr Glu Ala Gln Pro Met Leu Asn Leu Ala Met

180 185 190

Pro Ala Asp Ile Asp Glu Thr Ile Lys Ala His Ser Asp Ile Val Glu

195 200 205

Ala Leu Ser Ser Gly Asn Ile Asp Asp Tyr Arg Ser Ala Val Leu Ala

210 215 220

His Tyr Ala Pro Phe Arg Arg Met Ile Ser Asn Met Leu Asp Ala His

225 230 235 240

<210>53

<211>2749

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2719)

<223>RXA03136

<400>53

ggtacaagtc ccttttttcg tctagtattg atttatggtt gcctcagtct ctttcacccc 60

gtcatcgtcc caaacgttcc taccgcgggc gtcgatcatc gtg caa gaa gtc agc 115

Val Gln Glu Val Ser

1 5

aca cag atc aaa aga tta gaa tcc ggc gaa ggc aaa ctc ttc cac atc 163

Thr Gln Ile Lys Arg Leu Glu Ser Gly Glu Gly Lys Leu Phe His Ile

10 15 20

agt ggc cag ccc ggc gcg ggt aaa aca gag ttc ggc atg cag ctt atc 211

Ser Gly Gln Pro Gly Ala Gly Lys Thr Glu Phe Gly Met Gln Leu Ile

25 30 35

gac gag ctc caa ggc tgg acc gtg gta cgt gcc act tcc ctg tcg tgg 259

Asp Glu Leu Gln Gly Trp Thr Val Val Arg Ala Thr Ser Leu Ser Trp

40 45 50

ctg aaa aac agc ccc cga aac ctc ctt gga cac atc gcc cac aaa ttg 307

Leu Lys Asn Ser Pro Arg Asn Leu Leu Gly His Ile Ala His Lys Leu

55 60 65

ggt gcg cac tcc gcc aac tcc atc cgt ggt gtg atc gac cgt ctc gat 355

Gly Ala His Ser Ala Asn Ser Ile Arg Gly Val Ile Asp Arg Leu Asp

70 75 80 85

gcc tcc acc gtg gtg atc gtt gac gat gtc cac tgg gcc gac gtg gaa 403

Ala Ser Thr Val Val Ile Val Asp Asp Val His Trp Ala Asp Val Glu

90 95 100

tcc atg caa aaa ctc atc gaa tat tcc atg cgc atg gtt tct ggc cgt 451

Ser Met Gln Lys Leu Ile Glu Tyr Ser Met Arg Met Val Ser Gly Arg

105 110 115

ttc gca ctc atc atg att ggc ctt gat gaa gag aac tta gtg ttc cac 499

Phe Ala Leu Ile Met Ile Gly Leu Asp Glu Glu Asn Leu Val Phe His

120 125 130

gat gag gtg gtc tcg ctc ccc tcc atc gca gac tcc acc tac gta ttg 547

Asp Glu Val Val Ser Leu Pro Ser Ile Ala Asp Ser Thr Tyr Val Leu

135 140 145

ccg ccg atg agt att gaa gaa atc cgc cag ctt gcg ctt acc gat gtc 595

Pro Pro Met Ser Ile Glu Glu Ile Arg Gln Leu Ala Leu Thr Asp Val

150 155 160 165

cgc ggc cgc atc agc acc acc acc gcc aca gac atc cag cgc atc acc 643

Arg Gly Arg Ile Ser Thr Thr Thr Ala Thr Asp Ile Gln Arg Ile Thr

170 175 180

ggc ggc atc tac ggg cga gtc aaa gaa gtc ctc cac tcg gaa tcc ccc 691

Gly Gly Ile Tyr Gly Arg Val Lys Glu Val Leu His Ser Glu Ser Pro

185 190 195

gat cac tgg cga atg ccc aac cca aat att ccc atc cca caa agc tgg 739

Asp His Trp Arg Met Pro Asn Pro Asn Ile Pro Ile Pro Gln Ser Trp

200 205 210

cat gcc aac ctg ttg aga cgc atc acc aac gaa gaa gtc tgg cat gta 787

His Ala Asn Leu Leu Arg Arg Ile Thr Asn Glu Glu Val Trp His Val

215 220 225

cta ctc gcc gtc gct gtc ctt ccc tcc gga ggc ccc att gac ctg gta 835

Leu Leu Ala Val Ala Val Leu Pro Ser Gly Gly Pro Ile Asp Leu Val

230 235 240 245

aaa ctc ata ggc aac gac ccc acg ggc atg ctt tgc gac gac gcc gtc 883

Lys Leu Ile Gly Asn Asp Pro Thr Gly Met Leu Cys Asp Asp Ala Val

250 255 260

cgc tca ggc ctg ctc cgc gtg ctg ccg tct gac ggc caa cca caa gtg 931

Arg Ser Gly Leu Leu Arg Val Leu Pro Ser Asp Gly GIn Pro Gln Val

265 270 275

gat ttg gtc ctg ccg atc gac cgc gcc gta ctg caa tca cgc act ccg 979

Asp Leu Val Leu Pro Ile Asp Arg Ala Val Leu Gln Ser Arg Thr Pro

280 285 290

ctc aac att ctg gcg cag ttg cac cac aag gca gcc gaa tat tac ggc 1027

Leu Asn Ile Leu Ala Gln Leu His His Lys Ala Ala Glu Tyr Tyr Gly

295 300 305

aag tgg aat caa aaa gat gcc caa ctg gag cac gaa gca ttt gct gca 1075

Lys Trp Asn Gln Lys Asp Ala Gln Leu Glu His Glu Ala Phe Ala Ala

310 315 320 325

att gat cca aat gat cca gca gtg cga gcc cta gcg cag cgc gga tat 1123

Ile Asp Pro Asn Asp Pro Ala Val Arg Ala Leu Ala Gln Arg Gly Tyr

330 335 340

gcg ttg ggt agg act ggc cac tgg atg gaa tcg gca cac gcc cta tct 1171

Ala Leu Gly Arg Thr Gly His Trp Met Glu Ser Ala His Ala Leu Ser

345 350 355

ctt gcc gcg aac cgc act gca cac caa gaa gaa tca aat aag tac ttg 1219

Leu Ala Ala Asn Arg Thr Ala His Gln Glu Glu Ser Asn Lys Tyr Leu

360 365 370

ctg gag tcc atc gat tca ctg atc gcc gcc gcc gat ctc ccc caa gct 1267

Leu Glu Ser Ile Asp Ser Leu Ile Ala Ala Ala Asp Leu Pro Gln Ala

375 380 385

cga tcc aga gca tcc acc ctt gat ctt gga gaa acc ggc att caa caa 1315

Arg Ser Arg Ala Ser Thr Leu Asp Leu Gly Glu Thr Gly Ile Gln Gln

390 395 400 405

gac tca atg ctg ggc tac ctg gca atc cac gaa ggc cgg cgc ctc gaa 1363

Asp Ser Met Leu Gly Tyr Leu Ala Ile His Glu Gly Arg Arg Leu Glu

410 415 420

gca cgc aat ctc ctt cat cgt gct tct gaa gaa ttg ctg gcg cag cac 1411

Ala Arg Asn Leu Leu His Arg Ala Ser Glu Glu Leu Leu Ala Gln His

425 430 435

ccg att gat ccg atc cac ggc ccc cgc atg gct cag cgc aaa gta ctg 1459

Pro Ile Asp Pro Ile His Gly Pro Arg Met Ala Gln Arg Lys Val Leu

440 445 450

tta aac tta gtg gac tgg aat cca gaa gaa ctc ctg gtg tgg gct gat 1507

Leu Asn Leu Val Asp Trp Asn Pro Glu Glu Leu Leu Val Trp Ala Asp

455 460 465

aga gca gtc gca tgg act gaa gag gat gct ggc gaa aag gtt gag gcc 1555

Arg Ala Val Ala Trp Thr Glu Glu Asp Ala Gly Glu Lys Val Glu Ala

470 475 480 485

caa gct att tcc ctc att gga caa tcc atc ctc gat ggc tgc ctc ccc 1603

Gln Ala Ile Ser Leu Ile Gly Gln Ser Ile Leu Asp Gly Cys Leu Pro

490 495 500

gaa gat aaa ccc atc ccc ggt gaa acc acc ctt cac gca caa cgc cgc 1651

Glu Asp Lys Pro Ile Pro Gly Glu Thr Thr Leu His Ala Gln Arg Arg

505 510 515

cac atg gca atg ggc tgg ctt tcc atg gtt cac gat gat cca gta act 1699

His Met Ala Met Gly Trp Leu Ser Met Val His Asp Asp Pro Val Thr

520 525 530

gca cgt caa aag ctt gaa cgt cgc aca tcc atc aat ggt tca gaa cgc 1747

Ala Arg Gln Lys Leu Glu Arg Arg Thr Ser Ile Asn Gly Ser Glu Arg

535 540 545

atc agt ttg tgg caa gac gga tgg ctg gct cgg tcc cta ctg ctg ctc 1795

Ile Ser Leu Trp Gln Asp Gly Trp Leu Ala Arg Ser Leu Leu Leu Leu

550 555 560 565

ggc gaa tgg gag tcc gca gca cgc acc gta gaa atc ggt ctg gcc cgc 1843

Gly Glu Trp Glu Ser Ala Ala Arg Thr Val Glu Ile Gly Leu Ala Arg

570 575 580

gcc gaa cag ttt ggc atc cgc ttc ctc gaa cca ctg tta ctg tgg tcg 1891

Ala Glu Gln Phe Gly Ile Arg Phe Leu Glu Pro Leu Leu Leu Trp Ser

585 590 595

ggc gcc aca att gca aca gcc cgc gga aac tct gac ttg gca cga aat 1939

Gly Ala Thr Ile Ala Thr Ala Arg Gly Asn Ser Asp Leu Ala Arg Asn

600 605 610

tac atg agc aga ctg tcc acc gat caa gac tcc ttc atc gtc caa tct 1987

Tyr Met Ser Arg Leu Ser Thr Asp Gln Asp Ser Phe Ile Val Gln Ser

615 620 625

atg cca tct gcg atg tgt cgc atg tgg gtc cac cgc cat aga aat gaa 2035

Met Pro Ser Ala Met Cys Arg Met Trp Val His Arg His Arg Asn Glu

630 635 640 645

atc ccc ggt gcg atc gtg gcc gga gaa caa ttg gaa aaa atc gcc gca 2083

Ile Pro Gly Ala Ile Val Ala Gly Glu Gln Leu Glu Lys Ile Ala Ala

650 655 660

cac aaa cac gtc aac gca cct gga ttc tgg cca tgg caa gac gtc cac 2131

His Lys His Val Asn Ala Pro Gly Phe Trp Pro Trp Gln Asp Val His

665 670 675

gca acg cat ctc atc cgc atc ggc gaa act gag cgc gcc cag gag tta 2179

Ala Thr His Leu Ile Arg Ile Gly Glu Thr Glu Arg Ala Gln Glu Leu

680 685 690

gtg aac tcc acg ctt gag gag ctc aga ggc tcc gat atc atg tct gcc 2227

Val Asn Ser Thr Leu Glu Glu Leu Arg Gly Ser Asp Ile Met Ser Ala

695 700 705

cac gca aaa att gcc gtt ccc gac gcc atg ttg atg atc cac cac gga 2275

His Ala Lys Ile Ala Val Pro Asp Ala Met Leu Met Ile His His Gly

710 715 720 725

gat gtg aaa aag gga ttt aag cgt ttc gac gac gcc ctc gat atg ctc 2323

Asp Val Lys Lys Gly Phe Lys Arg Phe Asp Asp Ala Leu Asp Met Leu

730 735 740

gat ccc ctc acc ctc ccc tac tat cgg gca cgc atc tgc ttt gaa tac 2371

Asp Pro Leu Thr Leu Pro Tyr Tyr Arg Ala Arg Ile Cys Phe Glu Tyr

745 750 755

ggc cag gcc ctg aga cgc cag ggg caa cgt cga cgt gct gat gaa caa 2419

Gly Gln Ala Leu Arg Arg Gln Gly Gln Arg Arg Arg Ala Asp Glu Gln

760 765 770

ttt gcc cgt gca gct tcc cta ttc caa gac atg ggc gcc gac gcg atg 2467

Phe Ala Arg Ala Ala Ser Leu Phe Gln Asp Met Gly Ala Asp Ala Met

775 780 785

gtc acc cta gcc aac cga gaa cgc cgg gtg ggt ggc ctt ggt caa cga 2515

Val Thr Leu Ala Asn Arg Glu Arg Arg Val Gly Gly Leu Gly Gln Arg

790 795 800 805

tcc gag caa gcc ggt ggg ctc acc cct cag gaa tat gaa att gcc cga 2563

Ser Glu Gln Ala Gly Gly Leu Thr Pro Gln Glu Tyr Glu Ile Ala Arg

810 815 820

tta gtg tca tct ggg cat gcc aac cga gag gtc gca cag gag ctt ttc 2611

Leu Val Ser Ser Gly His Ala Asn Arg Glu Val Ala Gln Glu Leu Phe

825 830 835

ctc tcg cct aag acc gtg gaa tac cat ctc acc cgg gtg tac aaa aag 2659

Leu Ser Pro Lys Thr Val Glu Tyr His Leu Thr Arg Val Tyr Lys Lys

840 845 850

ctc gga ata cgc aat cgg atg gaa ctt gcc gag gct ttg aag aag tac 2707

Leu Gly Ile Arg Asn Arg Met Glu Leu Ala Glu Ala Leu Lys Lys Tyr

855 860 865

tca cac gac gcc tagcagcgga tatgtttgcg gacaagtatc 2749

Ser His Asp Ala

870

<210>54

<211>873

<212>PRT

<213>谷氨酸棒状杆菌

<400>54

Val Gln Glu Val Ser Thr Gln Ile Lys Arg Leu Glu Ser Gly Glu Gly

1 5 10 15

Lys Leu Phe His Ile Ser Gly Gln Pro Gly Ala Gly Lys Thr Glu Phe

20 25 30

Gly Met Gln Leu Ile Asp Glu Leu Gln Gly Trp Thr Val Val Arg Ala

35 40 45

Thr Ser Leu Ser Trp Leu Lys Asn Ser Pro Arg Asn Leu Leu Gly His

50 55 60

Ile Ala His Lys Leu Gly Ala His Ser Ala Asn Ser Ile Arg Gly Val

65 70 75 80

Ile Asp Arg Leu Asp Ala Ser Thr Val Val Ile Val Asp Asp Val His

85 90 95

Trp Ala Asp Val Glu Ser Met Gln Lys Leu Ile Glu Tyr Ser Met Arg

100 105 110

Met Val Ser Gly Arg Phe Ala Leu Ile Met Ile Gly Leu Asp Glu Glu

115 120 125

Asn Leu Val Phe His Asp Glu Val Val Ser Leu Pro Ser Ile Ala Asp

130 135 140

Ser Thr Tyr Val Leu Pro Pro Met Ser Ile Glu Glu Ile Arg Gln Leu

145 150 155 160

Ala Leu Thr Asp Val Arg Gly Arg Ile Ser Thr Thr Thr Ala Thr Asp

165 170 175

Ile Gln Arg Ile Thr Gly Gly Ile Tyr Gly Arg Val Lys Glu Val Leu

180 185 190

His Ser Glu Ser Pro Asp His Trp Arg Met Pro Asn Pro Asn Ile Pro

195 200 205

Ile Pro Gln Ser Trp His Ala Asn Leu Leu Arg Arg Ile Thr Asn Glu

210 215 220

Glu Val Trp His Val Leu Leu Ala Val Ala Val Leu Pro Ser Gly Gly

225 230 235 240

Pro Ile Asp Leu Val Lys Leu Ile Gly Asn Asp Pro Thr Gly Met Leu

245 250 255

Cys Asp Asp Ala Val Arg Ser Gly Leu Leu Arg Val Leu Pro Ser Asp

260 265 270

Gly Gln Pro Gln Val Asp Leu Val Leu Pro Ile Asp Arg Ala Val Leu

275 280 285

Gln Ser Arg Thr Pro Leu Asn Ile Leu Ala Gln Leu His His Lys Ala

290 295 300

Ala Glu Tyr Tyr Gly Lys Trp Asn Gln Lys Asp Ala Gln Leu Glu His

305 310 315 320

Glu Ala Phe Ala Ala Ile Asp Pro Asn Asp Pro Ala Val Arg Ala Leu

325 330 335

Ala Gln Arg Gly Tyr Ala Leu Gly Arg Thr Gly His Trp Met Glu Ser

340 345 350

Ala His Ala Leu Ser Leu Ala Ala Asn Arg Thr Ala His Gln Glu Glu

355 360 365

Ser Asn Lys Tyr Leu Leu Glu Ser Ile Asp Ser Leu Ile Ala Ala Ala

370 375 380

Asp Leu Pro Gln Ala Arg Ser Arg Ala Ser Thr Leu Asp Leu Gly Glu

385 390 395 400

Thr Gly Ile Gln Gln Asp Ser Met Leu Gly Tyr Leu Ala Ile His Glu

405 410 415

Gly Arg Arg Leu Glu Ala Arg Asn Leu Leu His Arg Ala Ser Glu Glu

420 425 430

Leu Leu Ala Gln His Pro Ile Asp Pro Ile His Gly Pro Arg Met Ala

435 440 445

Gln Arg Lys Val Leu Leu Asn Leu Val Asp Trp Asn Pro Glu Glu Leu

450 455 460

Leu Val Trp Ala Asp Arg Ala Val Ala Trp Thr Glu Glu Asp Ala Gly

465 470 475 480

Glu Lys Val Glu Ala Gln Ala Ile Ser Leu Ile Gly Gln Ser Ile Leu

485 490 495

Asp Gly Cys Leu Pro Glu Asp Lys Pro Ile Pro Gly Glu Thr Thr Leu

500 505 510

His Ala Gln Arg Arg His Met Ala Met Gly Trp Leu Ser Met Val His

515 520 525

Asp Asp Pro Val Thr Ala Arg Gln Lys Leu Glu Arg Arg Thr Ser Ile

530 535 540

Asn Gly Ser Glu Arg Ile Ser Leu Trp Gln Asp Gly Trp Leu Ala Arg

545 550 555 560

Ser Leu Leu Leu Leu Gly Glu Trp Glu Ser Ala Ala Arg Thr Val Glu

565 570 575

Ile Gly Leu Ala Arg Ala Glu Gln Phe Gly Ile Arg Phe Leu Glu Pro

580 585 590

Leu Leu Leu Trp Ser Gly Ala Thr Ile Ala Thr Ala Arg Gly Asn Ser

595 600 605

Asp Leu Ala Arg Asn Tyr Met Ser Arg Leu Ser Thr Asp Gln Asp Ser

610 615 620

Phe Ile Val Gln Ser Met Pro Ser Ala Met Cys Arg Met Trp Val His

625 630 635 640

Arg His Arg Asn Glu Ile Pro Gly Ala Ile Val Ala Gly Glu Gln Leu

645 650 655

Glu Lys Ile Ala Ala His Lys His Val Asn Ala Pro Gly Phe Trp Pro

660 665 670

Trp Gln Asp Val His Ala Thr His Leu Ile Arg Ile Gly Glu Thr Glu

675 680 685

Arg Ala Gln Glu Leu Val Asn Ser Thr Leu Glu Glu Leu Arg Gly Ser

690 695 700

Asp Ile Met Ser Ala His Ala Lys Ile Ala Val Pro Asp Ala Met Leu

705 710 715 720

Met Ile His His Gly Asp Val Lys Lys Gly Phe Lys Arg Phe Asp Asp

725 730 735

Ala Leu Asp Met Leu Asp Pro Leu Thr Leu Pro Tyr Tyr Arg Ala Arg

740 745 750

Ile Cys Phe Glu Tyr Gly Gln Ala Leu Arg Arg Gln Gly Gln Arg Arg

755 760 765

Arg Ala Asp Glu Gln Phe Ala Arg Ala Ala Ser Leu Phe Gln Asp Met

770 775 780

Gly Ala Asp Ala Met Val Thr Leu Ala Asn Arg Glu Arg Arg Val Gly

785 790 795 800

Gly Leu Gly Gln Arg Ser Glu Gln Ala Gly Gly Leu Thr Pro Gln Glu

805 810 815

Tyr Glu Ile Ala Arg Leu Val Ser Ser Gly His Ala Asn Arg Glu Val

820 825 830

Ala Gln Glu Leu Phe Leu Ser Pro Lys Thr Val Glu Tyr His Leu Thr

835 840 845

Arg Val Tyr Lys Lys Leu Gly Ile Arg Asn Arg Met Glu LeuAla Glu

850 855 860

Ala Leu Lys Lys Tyr Ser His Asp Ala

865 870

<210>55

<211>1117

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1087)

<223>RXA03201

<400>55

gcagggccgg gtcgtgatca cccacaacgg ctccagcgta gaactgattg acgatcttgc 60

acatgcagtc cgcgtagaaa aggttgaggg ctaacacacg atg aag ctt ctg gtt 115

Met Lys Leu Leu Val

1 5

acc ggt ggc gct ggc tat gtt ggc agc gtt gct gcc gct gtt ctt cta 163

Thr Gly Gly Ala Gly Tyr Val Gly Ser Val Ala Ala Ala Val Leu Leu

10 15 20

gaa cat ggg cac gat gtc aca atc att gac aat ttc agt acc ggc aac 211

Glu His Gly His Asp Val Thr Ile Ile Asp Asn Phe Ser Thr Gly Asn

25 30 35

cgt gaa gca gtc cct gct gac gct cgc ctg ata gaa ggt gac gtc aac 259

Arg Glu Ala Val Pro Ala Asp Ala Arg Leu Ile Glu Gly Asp Val Asn

40 45 50

gat gtc gtt gag gaa gtc ctt tct gaa ggc gga ttc gag ggt gtc gtc 307

Asp Val Val Glu Glu Val Leu Ser Glu Gly Gly Phe Glu Gly Val Val

55 60 65

cac ttc gct gct cgc tca ttg gta ggc gaa tca gtg gaa aag ccc aat 355

His Phe Ala Ala Arg Ser Leu Val Gly Glu Ser Val Glu Lys Pro Asn

70 75 80 85

gaa tac tgg cac gac aat gtg gtc acg gcg ctc acc ttg ctc gat gca 403

Glu Tyr Trp His Asp Asn Val Val Thr Ala Leu Thr Leu Leu Asp Ala

90 95 100

atg cgt gcc cat ggg gtg aac aac ctt gtg ttc tcc tcc act gct gct 451

Met Arg Ala His Gly Val Asn Asn Leu Val Phe Ser Ser Thr Ala Ala

105 110 115

acc tac ggt gaa cca gac gtt gtt ccc atc acc gag gac atg ccc acc 499

Thr Tyr Gly Glu Pro Asp Val Val Pro Ile Thr Glu Asp Met Pro Thr

120 125 130

cag ccc acc aat gct tat ggc gca acg aag ctg tcc atc gac tac gca 547

Gln Pro Thr Asn Ala Tyr Gly Ala Thr Lys Leu Ser Ile Asp Tyr Ala

135 140 145

att acg tcc tac gct gct gcc ttt ggt ctg gct gca acc agc ttg cgc 595

Ile Thr Ser Tyr Ala Ala Ala Phe Gly Leu Ala Ala Thr Ser Leu Arg

150 155 160 165

tac ttc aac gtt gct ggc gca tac gga aac atc ggt gaa aat cgt gaa 643

Tyr Phe Asn Val Ala Gly Ala Tyr Gly Asn Ile Gly Glu Asn Arg Glu

170 175 180

gtt gaa act cac ctc att cca cta gtg ctg cag gta gca acc ggt cac 691

Val Glu Thr His Leu Ile Pro Leu Val Leu Gln Val Ala Thr Gly His

185 190 195

cgc gag aaa acc ttc atg ttc ggc gat gac tgg cca act ccc gat gga 739

Arg Glu Lys Thr Phe Met Phe Gly Asp Asp Trp Pro Thr Pro Asp Gly

200 205 210

acc gcg gtc cgc gat tac atc cac att ctt gat ctt gcc aaa gca cac 787

Thr Ala Val Arg Asp Tyr Ile His Ile Leu Asp Leu Ala Lys Ala His

215 220 225

gtg ttg gct ttg gaa tcc aac gaa gcc ggt aag cac cgc att ttc aac 835

Val Leu Ala Leu Glu Ser Asn Glu Ala Gly Lys His Arg Ile Phe Asn

230 235 240 245

ctt ggt tcc gga gat ggc tac agc gtg aaa cag gtt gtg gag atg tgc 883

Leu Gly Ser Gly Asp Gly Tyr Ser Val Lys Gln Val Val Glu Met Cys

250 255 260

cgt gaa gta acc gga cat ccc atc ccg gca gag gtc gca ccc agg cgt 931

Arg Glu Val Thr Gly His Pro Ile Pro Ala Glu Val Ala Pro Arg Arg

265 270 275

gca gga gat cca gca aca cta att gca tct tct gag aag gca aag caa 979

Ala Gly Asp Pro Ala Thr Leu Ile Ala Ser Ser Glu Lys Ala Lys Gln

280 285 290

gaa ctg ggt tgg acc cct gag cac act gat ctg cgc acc atc gtt gag 1027

Glu Leu Gly Trp Thr Pro Glu His Thr Asp Leu Arg Thr Ile Val Glu

295 300 305

gac gct tgg gcg ttt aca tcc gca ctg ggc gat cgt tcc cat gct gca 1075

Asp Ala Trp Ala Phe Thr Ser Ala Leu Gly Asp Arg Ser His Ala Ala

310 315 320 325

aag aag aaa gct taagctttcg gatcggggac gtgaaagtcc 1117

Lys Lys Lys Ala

<210>56

<211>329

<212>PRT

<213>谷氨酸棒状杆菌

<400>56

Met Lys Leu Leu Val Thr Gly Gly Ala Gly Tyr Val Gly Ser Val Ala

1 5 10 15

Ala Ala Val Leu Leu Glu His Gly His Asp Val Thr Ile Ile Asp Asn

20 25 30

Phe Ser Thr Gly Asn Arg Glu Ala Val Pro Ala Asp Ala Arg Leu Ile

35 40 45

Glu Gly Asp Val Asn Asp Val Val Glu Glu Val Leu Ser Glu Gly Gly

50 55 60

Phe Glu Gly Val Val His Phe Ala Ala Arg Ser Leu Val Gly Glu Ser

65 70 75 80

Val Glu Lys Pro Asn Glu Tyr Trp His Asp Asn Val Val Thr Ala Leu

85 90 95

Thr Leu Leu Asp Ala Met Arg Ala His Gly Val Asn Asn Leu Val Phe

100 105 110

Ser Ser Thr Ala Ala Thr Tyr Gly Glu Pro Asp Val Val Pro Ile Thr

115 120 125

Glu Asp Met Pro Thr Gln Pro Thr Asn Ala Tyr Gly Ala Thr Lys Leu

130 135 140

Ser Ile Asp Tyr Ala Ile Thr Ser Tyr Ala Ala Ala Phe Gly Leu Ala

145 150 155 160

Ala Thr Ser Leu Arg Tyr Phe Asn Val Ala Gly Ala Tyr Gly Asn Ile

165 170 175

Gly Glu Asn Arg Glu Val Glu Thr His Leu Ile Pro Leu Val Leu Gln

180 185 190

Val Ala Thr Gly His Arg Glu Lys Thr Phe Met Phe Gly Asp Asp Trp

195 200 205

Pro Thr Pro Asp Gly Thr Ala Val Arg Asp Tyr Ile His Ile Leu Asp

210 215 220

Leu Ala Lys Ala His Val Leu Ala Leu Glu Ser Asn Glu Ala Gly Lys

225 230 235 240

His Arg Ile Phe Asn Leu Gly Ser Gly Asp Gly Tyr Ser Val Lys Gln

245 250 255

Val Val Glu Met Cys Arg Glu Val Thr Gly His Pro Ile Pro Ala Glu

260 265 270

Val Ala Pro Arg Arg Ala Gly Asp Pro Ala Thr Leu Ile Ala Ser Ser

275 280 285

Glu Lys Ala Lys Gln Glu Leu Gly Trp Thr Pro Glu His Thr Asp Leu

290 295 300

Arg Thr Ile Val Glu Asp Ala Trp Ala Phe Thr Ser Ala Leu Gly Asp

305 310 315 320

Arg Ser His Ala Ala Lys Lys Lys Ala

325

<210>57

<211>463

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(433)

<223>RXA03407

<400>57

gcccgaaaag tcctcaaagc atggaaagcc aaacagtgac cattgataga atatgtgtca 60

aaacatatat ttggatttgt gcttttactt gtctcgcgaa ttg gca ata gga gtc 115

Leu Ala Ile Gly Val

1 5

agc aac atg tca tta aag agt ttt agt tct cta gca caa tca gag aag 163

Ser Asn Met Ser Leu Lys Ser Phe Ser Ser Leu Ala Gln Ser Glu Lys

10 15 20

tca aca tgg agt aaa gaa gct aag caa gat tac gag gac gct tcc gcg 211

Ser Thr Trp Ser Lys Glu Ala Lys Gln Asp Tyr Glu Asp Ala Ser Ala

25 30 35

cta atc act caa gaa ctc gcc tca cga aaa gct ctt ggc caa aaa cta 259

Leu Ile Thr Gln Glu Leu Ala Ser Arg Lys Ala Leu Gly Gln Lys Leu

40 45 50

gtc aag gct cgg aaa caa cga gga gtc acc caa gtt caa ctt gct gaa 307

Val Lys Ala Arg Lys Gln Arg Gly Val Thr Gln Val Gln Leu Ala Glu

55 60 65

gct tct gga gtc caa cag gca gaa ata agc aag att gaa cgt ggc ctc 355

Ala Ser Gly Val Gln Gln Ala Glu Ile Ser Lys Ile Glu Arg Gly Leu

70 75 80 85

gcc aat ccc act ttt tcc aca ctt gaa agc ctc gca tcc cac cta gga 403

Ala Asn Pro Thr Phe Ser Thr Leu Glu Ser Leu Ala Ser His Leu Gly

90 95 100

ctt caa ttc act ttt aca gaa tca gcc gca tagcatccaa cccttaataa 453

Leu Gln Phe Thr Phe Thr Glu Ser Ala Ala

105 110

ataactccaa 463

<210>58

<211>111

<212>PRT

<213>谷氨酸棒状杆菌

<400>58

Leu Ala Ile Gly Val Ser Asn Met Ser Leu Lys Ser Phe Ser Ser Leu

1 5 10 15

Ala Gln Ser Glu Lys Ser Thr Trp Ser Lys Glu Ala Lys Gln Asp Tyr

20 25 30

Glu Asp Ala Ser Ala Leu Ile Thr Gln Glu Leu Ala Ser Arg Lys Ala

35 40 45

Leu Gly Gln Lys Leu Val Lys Ala Arg Lys Gln Arg Gly Val Thr Gln

50 55 60

Val Gln Leu Ala Glu Ala Ser Gly Val Gln Gln Ala Glu Ile Ser Lys

65 70 75 80

Ile Glu Arg Gly Leu Ala Asn Pro Thr Phe Ser Thr Leu Glu Ser Leu

85 90 95

Ala Ser His Leu Gly Leu Gln Phe Thr Phe Thr Glu Ser Ala Ala

100 105 110

<210>59

<211>487

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(457)

<223>RXA03629

<400>59

tgatggatca ccagtagatt aattcgtctt aacatcaacg aactaccccc attggcgagt 60

ttttagaaaa aattgaatga gaggatataa ggtaaaactc atg acg cac agt aag 115

Met Thr His Ser Lys

1 5

aaa gcg atg aaa gct aaa gct aaa gct ctt ctg gct tcc cag cgt gaa 163

Lys Ala Met Lys Ala Lys Ala Lys Ala Leu Leu Ala Ser Gln Arg Glu

10 15 20

ttc ctt gac tca ctt gta gct ttg aga aag aaa gct ggc att agc caa 211

Phe Leu Asp Ser Leu Val Ala Leu Arg Lys Lys Ala Gly Ile Ser Gln

25 30 35

gac gag gtg gct aat cgg atg ggc gtt tcc cag agt gct att tct caa 259

Asp Glu Val Ala Asn Arg Met Gly Val Ser Gln Ser Ala Ile Ser Gln

40 45 50

ttt gag cac tac gat gca aat ccc act ctg tct acg atc cga cgc tat 307

Phe Glu His Tyr Asp Ala Asn Pro Thr Leu Ser Thr Ile Arg Arg Tyr

55 60 65

gcg cta gcg gta gat gct tct ata tct tat agg gtt agc tct tct gcc 355

Ala Leu Ala Val Asp Ala Ser Ile Ser Tyr Arg Val Ser Ser Ser Ala

70 75 80 85

acc ctc tat caa gag tat gag tcg agg aca aac aat cac gta tcc gtg 403

Thr Leu Tyr Gln Glu Tyr Glu Ser Arg Thr Asn Asn His Val Ser Val

90 95 100

tcc ggc cag gaa gct gcc ccg ccc tac gtt gat tgg gaa aag ccc att 451

Ser Gly Gln Glu Ala Ala Pro Pro Tyr Val Asp Trp Glu Lys Pro Ile

105 ll0 115

ttg gcg tagtgtttcg ggttttatag gttgagattg 487

Leu Ala

<210>60

<211>119

<212>PRT

<213>谷氨酸棒状杆菌

<400>60

Met Thr His Ser Lys Lys Ala Met Lys Ala Lys Ala Lys Ala Leu Leu

1 5 10 15

Ala Ser Gln Arg Glu Phe Leu Asp Ser Leu Val Ala Leu Arg Lys Lys

20 25 30

Ala Gly Ile Ser Gln Asp Glu Val Ala Asn Arg Met Gly Val Ser Gln

35 40 45

Ser Ala Ile Ser Gln Phe Glu His Tyr Asp Ala Asn Pro Thr Leu Ser

50 55 60

Thr Ile Arg Arg Tyr Ala Leu Ala Val Asp Ala Ser Ile Ser Tyr Arg

65 70 75 80

Val Ser Ser Ser Ala Thr Leu Tyr Gln Glu Tyr Glu Ser Arg Thr Asn

85 90 95

Asn His Val Ser Val Ser Gly Gln Glu Ala Ala Pro Pro Tyr Val Asp

100 105 110

Trp Glu Lys Pro Ile Leu Ala

115

<210>61

<211>892

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(862)

<223>RXA03928

<400>61

tggtggcggc gggctgcgtc gaaaagcgaa aatcaacaag tttgcaacac ctcagtgcca 60

agagtggtta aggtgatggt gatcacgcta tagttgcgcc atg gga aag aca tat 115

Met Gly Lys Thr Tyr

1 5

gtg ggg tcc agg ctg cgc caa ctg cgc cgc gaa aga gac ctg agc cag 163

Val Gly Ser Arg Leu Arg Gln Leu Arg Arg Glu Arg Asp Leu Ser Gln

10 15 20

gca tcc tta gca gca acc ctt ggc tta tct gca agt tat gta aat cag 211

Ala Ser Leu Ala Ala Thr Leu Gly Leu Ser Ala Ser Tyr Val Asn Gln

25 30 35

att gag cac gac gta cgc ccg ctc acc gta ccg gtg tta ttg cgc atc 259

Ile Glu His Asp Val Arg Pro Leu Thr Val Pro Val Leu Leu Arg Ile

40 45 50

acc gag gcg ttc ggc gta gac gca acg ttt ttc tcc cgc gac gat gac 307

Thr Glu Ala Phe Gly Val Asp Ala Thr Phe Phe Ser Arg Asp Asp Asp

55 60 65

tcc cgc ctg ctc gcc gag gtc caa gac gtc atg ctg gac cgg gag atc 355

Ser Arg Leu Leu Ala Glu Val Gln Asp Val Met Leu Asp Arg Glu Ile

70 75 80 85

aat cct gcg aac gtg gag ctg caa gag ctt tcg gag atg gtg tac aac 403

Asn Pro Ala Asn Val Glu Leu Gln Glu Leu Ser Glu Met Val Tyr Asn

90 95 100

cac ccc caa cta gcg cgc gcg atg gtg gaa atg cac cag cgt tac cga 451

His Pro Gln Leu Ala Arg Ala Met Val Glu Met His Gln Arg Tyr Arg

105 110 115

aac gtg cgc gat aag ttc tcc atc gca gtg gat aat cgc acc aac acg 499

Asn Val Arg Asp Lys Phe Ser Ile Ala Val Asp Asn Arg Thr Asn Thr

120 125 130

cct gag gaa cgc cgt ccc atc gcg gag gcc gtg agc atg ccg cac gaa 547

Pro Glu Glu Arg Arg Pro Ile Ala Glu Ala Val Ser Met Pro His Glu

135 140 145

gag gtc cgc gat ttc att tac gcc cgc caa aac tac ttc gat gcc ctt 595

Glu Val Arg Asp Phe Ile Tyr Ala Arg Gln Asn Tyr Phe Asp Ala Leu

150 155 160 165

gac cgc cgc gcc gaa gcc atg cgc cgc gca act ggg ctg gca gcc gta 643

Asp Arg Arg Ala Glu Ala Met Arg Arg Ala Thr Gly Leu Ala Ala Val

170 175 180

cga ttc ccg cgc cat gga aga ttc gat cgc ccg ccg cct gca aat gga 691

Arg Phe Pro Arg His Gly Arg Phe Asp Arg Pro Pro Pro Ala Asn Gly

185 190 195

tca cga tgt cac cat cac ctc ctc caa aga gga atc cgg cac gct gca 739

Ser Arg Cys His His His Leu Leu Gln Arg Gly Ile Arg His Ala Ala

200 205 210

cca ctt cga ccc cga gac gcg tct gct gac aat cca cgc acg cct caa 787

Pro Leu Arg Pro Arg Asp Ala Ser Ala Asp Asn Pro Arg Thr Pro Gln

215 220 225

ccc cgg gca acg cgc ctt ccg cat ggc cac cga act cgg cta cct aga 835

Pro Arg Ala Thr Arg Leu Pro His Gly His Arg Thr Arg Leu Pro Arg

230 235 240 245

agc caa cga cct cat cga agg tat cgt tgacgacggc atctggtcca 882

Ser Gln Arg Pro His Arg Arg Tyr Arg

250

cccccgaagc 892

<210>62

<211>254

<212>PRT

<213>谷氨酸棒状杆菌

<400>62

Met Gly Lys Thr Tyr Val Gly Ser Arg Leu Arg Gln Leu Arg Arg Glu

1 5 10 15

Arg Asp Leu Ser Gln Ala Ser Leu Ala Ala Thr Leu Gly Leu Ser Ala

20 25 30

Ser Tyr Val Asn Gln Ile Glu His Asp Val Arg Pro Leu Thr Val Pro

35 40 45

Val Leu Leu Arg Ile Thr Glu Ala Phe Gly Val Asp Ala Thr Phe Phe

50 55 60

Ser Arg Asp Asp Asp Ser Arg Leu Leu Ala Glu Val Gln Asp Val Met

65 70 75 80

Leu Asp Arg Glu Ile Asn Pro Ala Asn Val Glu Leu Gln Glu Leu Ser

85 90 95

Glu Met Val Tyr Asn His Pro Gln Leu Ala Arg Ala Met Val Glu Met

100 105 110

His Gln Arg Tyr Arg Asn Val Arg Asp Lys Phe Ser Ile Ala Val Asp

115 120 125

Asn Arg Thr Asn Thr Pro Glu Glu Arg Arg Pro Ile Ala Glu Ala Val

130 135 140

Ser Met Pro His Glu Glu Val Arg Asp Phe Ile Tyr Ala Arg Gln Asn

145 150 155 160

Tyr Phe Asp Ala Leu Asp Arg Arg Ala Glu Ala Met Arg Arg Ala Thr

165 170 175

Gly Leu Ala Ala Val Arg Phe Pro Arg His Gly Arg Phe Asp Arg Pro

180 185 190

Pro Pro Ala Asn Gly Ser Arg Cys His His His Leu Leu Gln Arg Gly

195 200 205

Ile Arg His Ala Ala Pro Leu Arg Pro Arg Asp Ala Ser Ala Asp Asn

210 215 220

Pro Arg Thr Pro Gln Pro Arg Ala Thr Arg Leu Pro His Gly His Arg

225 230 235 240

Thr Arg Leu Pro Arg Ser Gln Arg Pro His Arg Arg Tyr Arg

245 250

<210>63

<211>499

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(469)

<223>RXA04129

<400>63

ctattttttc atttccctca taaaaggttt atatagaagg taaaatagca agcgtgctag 60

atccattcca gacctagcct aaggacggaa ggacttcccc gtg gct caa cac tca 115

Val Ala Gln His Ser

1 5

aat cgg aat ttc gtc acc cct gca gaa gag tcc gaa tcc aca gca tca 163

Asn Arg Asn Phe Val Thr Pro Ala Glu Glu Ser Glu Ser Thr Ala Ser

10 15 20

gct gag ctt cag aaa ctt gca aca gca aaa aac atc aaa gcg atc agc 211

Ala Glu Leu Gln Lys Leu Ala Thr Ala Lys Asn Ile Lys Ala Ile Ser

25 30 35

ctg ctg att agg gca cta gat tcc ccc tta aga atc gaa atc atc ctc 259

Leu Leu Ile Arg Ala Leu Asp Ser Pro Leu Arg Ile Glu Ile Ile Leu

40 45 50

gcc ctc aat gaa agg ccc cac tac gtc cac gaa ttg gtc aag cta gta 307

Ala Leu Asn Glu Arg Pro His Tyr Val His Glu Leu Val Lys Leu Val

55 60 65

aaa agt tcg caa cca cta gtg agc cag cac ctc aaa gtc ctt aaa act 355

Lys Ser Ser Gln Pro Leu Val Ser Gln His Leu Lys Val Leu Lys Thr

70 75 80 85

gca ggt atc gtc gac gca gaa cgt caa ggc cgg caa atg act tat tca 403

Ala Gly Ile Val Asp Ala Glu Arg Gln Gly Arg Gln Met Thr Tyr Ser

90 95 100

ctt gcc gaa cca ctc gtc ctc gac ata ctt ctc ctg gca cta aac gca 451

Leu Ala Glu Pro Leu Val Leu Asp Ile Leu Leu Leu Ala Leu Asn Ala

105 110 115

ggg gtt gac aca tct ggg tagactatcg aagtacattt tgtgtcattg 499

Gly Val Asp Thr Ser Gly

120

<210>64

<211>123

<212>PRT

<213>谷氨酸棒状杆菌

<400>64

Val Ala Gln His Ser Asn Arg Asn Phe Val Thr Pro Ala Glu Glu Ser

1 5 10 15

Glu Ser Thr Ala Ser Ala Glu Leu Gln Lys Leu Ala Thr Ala Lys Asn

20 25 30

Ile Lys Ala Ile Ser Leu Leu Ile Arg Ala Leu Asp Ser Pro Leu Arg

35 40 45

Ile Glu Ile Ile Leu Ala Leu Asn Glu Arg Pro His Tyr Val His Glu

50 55 60

Leu Val Lys Leu Val Lys Ser Ser Gln Pro Leu Val Ser Gln His Leu

65 70 75 80

Lys Val Leu Lys Thr Ala Gly Ile Val Asp Ala Glu Arg Gln Gly Arg

85 90 95

Gln Met Thr Tyr Ser Leu Ala Glu Pro Leu Val Leu Asp Ile Leu Leu

100 105 110

Leu Ala Leu Asn Ala Gly Val Asp Thr Ser Gly

115 120

<210>65

<211>784

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(754)

<223>RXA04350

<400>65

catcggggga aacggtgttg caacgcacca tgcgggtgtt gcactgcttt gatgcagaaa 60

acgcgaggtt ggggcctcgg aaatcgcgcg tcgcagtggt ttg cct agc tcg acg 115

Leu Pro Ser Ser Thr

1 5

gcg cac cgt ttg gtc gtg gaa ttg acg tcg gtg aag ttg ctg gag cgc 163

Ala His Arg Leu Val Val Glu Leu Thr Ser Val Lys Leu Leu Glu Arg

10 15 20

ttt tct gac ggc aaa tat ggc atc ggc acg cat gcg tgg gag acg ttt 211

Phe Ser Asp Gly Lys Tyr Gly Ile Gly Thr His Ala Trp Glu Thr Phe

25 30 35

gtg agg gcg aat cct ttg gag cgc atg agg ctg caa gct cag acc atc 259

Val Arg Ala Asn Pro Leu Glu Arg Met Arg Leu Gln Ala Gln Thr Ile

40 45 50

ttg agt gat gtg cgt gaa gag ctg ggg caa tac gtg tgt ttg gcg gtg 307

Leu Ser Asp Val Arg Glu Glu Leu Gly Gln Tyr Val Cys Leu Ala Val

55 60 65

ccg gat ttt gtg gat cgt acg att ttg tat gtg gag cgt ttc gat tcg 355

Pro Asp Phe Val Asp Arg Thr Ile Leu Tyr Val Glu Arg Phe Asp Ser

70 75 80 85

acg gat agt cag ctg cgt ctt ttg ggc agg cat gcg ggg cgt ttg gat 403

Thr Asp Ser Gln Leu Arg Leu Leu Gly Arg His Ala Gly Arg Leu Asp

90 95 100

ttg cac acc aca tca ttg ggg ttg gtg atg ttg gcg ttt gct tcg ccg 451

Leu His Thr Thr Ser Leu Gly Leu Val Met Leu Ala Phe Ala Ser Pro

105 110 115

caa acc att aag gca gtg acg agt tcg ccg ttt aag gat tcc att tcg 499

Gln Thr Ile Lys Ala Val Thr Ser Ser Pro Phe Lys Asp Ser Ile Ser

120 125 130

ggg gat att acg gat ggt gct gct gta gct gcg atg ctc ccg gaa att 547

Gly Asp Ile Thr Asp Gly Ala Ala Val Ala Ala Met Leu Pro Glu Ile

135 140 145

cgc gcg acg ggg cat ttt gta ttt gtg ggt ggt ctg att ccg gag aat 595

Arg Ala Thr Gly His Phe Val Phe Val Gly Gly Leu Ile Pro Glu Asn

150 155 160 165

acg gcg gtt gcg gct ccg gtt ttt gat cga aaa ggg gtt gtt cgg gca 643

Thr Ala Val Ala Ala Pro Val Phe Asp Arg Lys Gly Val Val Arg Ala

170 175 180

tca gtg ggg gta gtg gcg agg aac gat gaa att gat gtg aat cag gcg 691

Ser Val Gly Val Val Ala Arg Asn Asp Glu Ile Asp Val Asn Gln Ala

185 190 195

gtt tct gtg gtg ctt gat gca tgc caa aag ctc agc gta agg cta caa 739

Val Ser Val Val Leu Asp Ala Cys Gln Lys Leu Ser Val Arg Leu Gln

200 205 210

aaa gac tct cta tat taagttcgca tttcgaacgc gcccatttgg 784

Lys Asp Ser Leu Tyr

215

<210>66

<211>218

<212>PRT

<213>谷氨酸棒状杆菌

<400>66

Leu Pro Ser Ser Thr Ala His Arg Leu Val Val Glu Leu Thr Ser Val

1 5 10 15

Lys Leu Leu Glu Arg Phe Ser Asp Gly Lys Tyr Gly Ile Gly Thr His

20 25 30

Ala Trp Glu Thr Phe Val Arg Ala Asn Pro Leu Glu Arg Met Arg Leu

35 40 45

Gln Ala Gln Thr Ile Leu Ser Asp Val Arg Glu Glu Leu Gly Gln Tyr

50 55 60

Val Cys Leu Ala Val Pro Asp Phe Val Asp Arg Thr Ile Leu Tyr Val

65 70 75 80

Glu Arg Phe Asp Ser Thr Asp Ser Gln Leu Arg Leu Leu Gly Arg His

85 90 95

Ala Gly Arg Leu Asp Leu His Thr Thr Ser Leu Gly Leu Val Met Leu

100 105 110

Ala Phe Ala Ser Pro Gln Thr Ile Lys Ala Val Thr Ser Ser Pro Phe

115 120 125

Lys Asp Ser Ile Ser Gly Asp Ile Thr Asp Gly Ala Ala Val Ala Ala

130 135 140

Met Leu Pro Glu Ile Arg Ala Thr Gly His Phe Val Phe Val Gly Gly

145 150 155 160

Leu Ile Pro Glu Asn Thr Ala Val Ala Ala Pro Val Phe Asp Arg Lys

165 170 175

Gly Val Val Arg Ala Ser Val Gly Val Val Ala Arg Asn Asp Glu Ile

180 185 190

Asp Val Asn Gln Ala Val Ser Val Val Leu Asp Ala Cys Gln Lys Leu

195 200 205

Ser Val Arg Leu Gln Lys Asp Ser Leu Tyr

210 215

<210>67

<211>904

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(874)

<223>RXA04363

<400>67

cacaagtcct cccgattctg gtgcgtgtga tgtcataaac acataatcga ggtgagccaa 60

gttacaatca aagggattcc gctatctgga aagagtgatt atg gat aac gtc gcc 115

Met Asp Asn Val Ala

1 5

ccc acc cag ggg tta ccc ccg aaa gaa ttt cta agc tct gtc gac att 163

Pro Thr Gln Gly Leu Pro Pro Lys Glu Phe Leu Ser Ser Val Asp Ile

10 15 20

gca ttg cag ctc att ttg ctg ctc cgt gac tct gga agt ttg acc att 211

Ala Leu Gln Leu Ile Leu Leu Leu Arg Asp Ser Gly Ser Leu Thr Ile

25 30 35

tcc ggt gcc gcc gaa acc ctt ggg gtg ggc gcg tct acg atc cat cga 259

Ser Gly Ala Ala Glu Thr Leu Gly Val Gly Ala Ser Thr Ile His Arg

40 45 50

tcc atg tca atg ctg gtc tac cgg ggt ttt gca gtc aga agc gag tcc 307

Ser Met Ser Met Leu Val Tyr Arg Gly Phe Ala Val Arg Ser Glu Ser

55 60 65

cgc acc tac ctt cca ggc tct gca ttg gcg acc tcc gcg ctg cag cca 355

Arg Thr Tyr Leu Pro Gly Ser Ala Leu Ala Thr Ser Ala Leu Gln Pro

70 75 80 85

ggc ctt ggc gct gac ttg acg aaa aaa tgc agc cac tac atg gaa tca 403

Gly Leu Gly Ala Asp Leu Thr Lys Lys Cys Ser His Tyr Met Glu Ser

90 95 100

atc ggc aag gaa act ggc gaa aca acc cac ttg gtg att ctg cag gga 451

Ile Gly Lys Glu Thr Gly Glu Thr Thr His Leu Val Ile Leu Gln Gly

105 110 115

gat agc gtt cac ttt att cac agt gtt gaa ggt tcc ctg ccg gtg cgc 499

Asp Ser Val His Phe Ile His Ser Val Glu Gly Ser Leu Pro Val Arg

120 125 130

gtg ggc aat cgc cga ggt caa gtc atg ccc gcc atc caa aat tca ggt 547

Val Gly Asn Arg Arg Gly Gln Val Met Pro Ala Ile Gln Asn Ser Gly

135 140 145

gga tta gtg atg ctt gca gag atg tca gcc cgg gag ctt cgg gca ctg 595

Gly Leu Val Met Leu Ala Glu Met Ser Ala Arg Glu Leu Arg Ala Leu

150 155 160 165

tat tcc agc ctg ggc gat gag gaa ttt gag aat tta aga aag cgt ctt 643

Tyr Ser Ser Leu Gly Asp Glu Glu Phe Glu Asn Leu Arg Lys Arg Leu

170 175 180

cgc agg acc cgg gat cga ggc cat ggc gca aac ttt ggc ttc ttt gag 691

Arg Arg Thr Arg Asp Arg Gly His Gly Ala Asn Phe Gly Phe Phe Glu

185 190 195

cag gac gtt agt gca gtt gcg gag cct tta ctc aac gat gtg ggt gat 739

Gln Asp Val Ser Ala Val Ala Glu Pro Leu Leu Asn Asp Val Gly Asp

200 205 210

gtt tta ggt gca att aca gtg gct gtg ccg tcg aat cgg ttc cgg gag 787

Val Leu Gly Ala Ile Thr Val Ala Val Pro Ser Asn Arg Phe Arg Glu

215 220 225

gtc tat ccg aag gcg gtg cag gtt ttg gaa cga cat atg cgg gat ctg 835

Val Tyr Pro Lys Ala Val Gln Val Leu Glu Arg His Met Arg Asp Leu

230 235 240 245

aac aag gct tta gct gat tac cga gtg ccc gaa aaa ggg tgaatggggg 884

Asn Lys Ala Leu Ala Asp Tyr Arg Val Pro Glu Lys Gly

250 255

gaattttcag agctgtcaca 904

<210>68

<211>258

<212>PRT

<213>谷氨酸棒状杆菌

<400>68

Met Asp Asn Val Ala Pro Thr Gln Gly Leu Pro Pro Lys Glu Phe Leu

1 5 10 15

Ser Ser Val Asp Ile Ala Leu Gln Leu Ile Leu Leu Leu Arg Asp Ser

20 25 30

Gly Ser Leu Thr Ile Ser Gly Ala Ala Glu Thr Leu Gly Val Gly Ala

35 40 45

Ser Thr Ile His Arg Ser Met Ser Met Leu Val Tyr Arg Gly Phe Ala

50 55 60

Val Arg Ser Glu Ser Arg Thr Tyr Leu Pro Gly Ser Ala Leu Ala Thr

65 70 75 80

Ser Ala Leu Gln Pro Gly Leu Gly Ala Asp Leu Thr Lys Lys Cys Ser

85 90 95

His Tyr Met Glu Ser Ile Gly Lys Glu Thr Gly Glu Thr Thr His Leu

100 105 110

Val Ile Leu Gln Gly Asp Ser Val His Phe Ile His Ser Val Glu Gly

115 120 125

Ser Leu Pro Val Arg Val Gly Asn Arg Arg Gly Gln Val Met Pro Ala

130 135 140

Ile Gln Asn Ser Gly Gly Leu Val Met Leu Ala Glu Met Ser Ala Arg

145 150 155 160

Glu Leu Arg Ala Leu Tyr Ser Ser Leu Gly Asp Glu Glu Phe Glu Asn

165 170 175

Leu Arg Lys Arg Leu Arg Arg Thr Arg Asp Arg Gly His Gly Ala Asn

180 185 190

Phe Gly Phe Phe Glu Gln Asp Val Ser Ala Val Ala Glu Pro Leu Leu

195 200 205

Asn Asp Val Gly Asp Val Leu Gly Ala Ile Thr Val Ala Val Pro Ser

210 215 220

Asn Arg Phe Arg Glu Val Tyr Pro Lys Ala Val Gln Val Leu Glu Arg

225 230 235 240

His Met Arg Asp Leu Asn Lys Ala Leu Ala Asp Tyr Arg Val Pro Glu

245 250 255

Lys Gly

<210>69

<211>973

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(943)

<223>RXA04620

<400>69

cccaaagggg aagtacactg tacccttgtc gaatgattgt tactcgtgac gcgccctatg 60

ggtgtaccag cacgggtgta aagcaggagg aaatctgaag gtg gat acc cag cgg 115

Val Asp Thr Gln Arg

1 5

att aaa gat gac gaa gat gct att cgt tcg gcg ctg aca tcg ctg aaa 163

Ile Lys Asp Asp Glu Asp Ala Ile Arg Ser Ala Leu Thr Ser Leu Lys

10 15 20

acc gca aca ggc atc cca gtc acc atg ttc gcc act gtg ttg cag gac 211

Thr Ala Thr Gly Ile Pro Val Thr Met Phe Ala Thr Val Leu Gln Asp

25 30 35

aat cgc ctg caa att act cag tgg gtt ggg ttg cgt acc ccg gct ctg 259

Asn Arg Leu Gln Ile Thr Gln Trp Val Gly Leu Arg Thr Pro Ala Leu

40 45 50

cag aat ctg gtc att gaa cca ggt gtg ggc gtt ggt gga cgc gtc gtc 307

Gln Asn Leu Val Ile Glu Pro Gly Val Gly Val Gly Gly Arg Val Val

55 60 65

gca acc cgt cgt ccg gtt ggt gtg agt gat tac acc agg gca aat gtc 355

Ala Thr Arg Arg Pro Val Gly Val Ser Asp Tyr Thr Arg Ala Asn Val

70 75 80 85

att tca cat gag aag gat tcc gcg att cag gat gag ggc ctt cat tcc 403

Ile Ser His Glu Lys Asp Ser Ala Ile Gln Asp Glu Gly Leu His Ser

90 95 100

att gtc gca gtt ccc gtg atc gtg cac cgc gaa att cgt ggc gtt ttg 451

Ile Val Ala Val Pro Val Ile Val His Arg Glu Ile Arg Gly Val Leu

105 110 115

tat gtt ggc gtt cac tct gcg gtg cgt ctc ggc gac act gtt att gaa 499

Tyr Val Gly Val His Ser Ala Val Arg Leu Gly Asp Thr Val Ile Glu

120 125 130

gaa gtc acc atg act gcg cgc acg ttg gaa caa aac ctg gcg atc aac 547

Glu Val Thr Met Thr Ala Arg Thr Leu Glu Gln Asn Leu Ala Ile Asn

135 140 145

tcc gcg ctt cgc cgc aat ggc gtt cct gat ggt cgc ggt tcc ctc aaa 595

Ser Ala Leu Arg Arg Asn Gly Val Pro Asp Gly Arg Gly Ser Leu Lys

150 155 160 165

gct aac cgc gtg atg aat ggg gcg gag tgg gag cag gtt cgt tcc act 643

Ala Asn Arg Val Met Asn Gly Ala Glu Trp Glu Gln Val Arg Ser Thr

170 175 180

cat tcc aag ctg cgc atg ctg gca aat cgt gtg acc gat gag gat ctg 691

His Ser Lys Leu Arg Met Leu Ala Asn Arg Val Thr Asp Glu Asp Leu

185 190 195

cgc cgc gat ttg gaa gag ctt tgc gat cag atg gtc acc cca gtc cgc 739

Arg Arg Asp Leu Glu Glu Leu Cys Asp Gln Met Val Thr Pro Val Arg

200 205 210

atc aag cag acc acc aag ctg tcc gcg cgt gag ttg gac gtg ctg gct 787

Ile Lys Gln Thr Thr Lys Leu Ser Ala Arg Glu Leu Asp Val Leu Ala

215 220 225

tgt gtc gcg ctc ggt cac acc aac gtc gaa gct gct gaa gag atg ggc 835

Cys Val Ala Leu Gly His Thr Asn Val Glu Ala Ala Glu Glu Met Gly

230 235 240 245

atc ggc gcg gaa acc gtc aag agc tac ctg cgc tcg gtc atg cgc aag 883

Ile Gly Ala Glu Thr Val Lys Ser Tyr Leu Arg Ser Val Met Arg Lys

250 255 260

ctc ggc gcc cac acg cgc tac gag gca gtc aac gca gca cgc cgg atc 931

Leu Gly Ala His Thr Arg Tyr Glu Ala Val Asn Ala Ala Arg Arg Ile

265 270 275

ggc gca ctg cct taaaaagatt ttgctttacg acgccaccct 973

Gly Ala Leu Pro

280

<210>70

<211>281

<212>PRT

<213>谷氨酸棒状杆菌

<400>70

Val Asp Thr Gln Arg Ile Lys Asp Asp Glu Asp Ala Ile Arg Ser Ala

1 5 10 15

Leu Thr Ser Leu Lys Thr Ala Thr Gly Ile Pro Val Thr Met Phe Ala

20 25 30

Thr Val Leu Gln Asp Asn Arg Leu Gln Ile Thr Gln Trp Val Gly Leu

35 40 45

Arg Thr Pro Ala Leu Gln Asn Leu Val Ile Glu Pro Gly Val Gly Val

50 55 60

Gly Gly Arg Val Val Ala Thr Arg Arg Pro Val Gly Val Ser Asp Tyr

65 70 75 80

Thr Arg Ala Asn Val Ile Ser His Glu Lys Asp Ser Ala Ile Gln Asp

85 90 95

Glu Gly Leu His Ser Ile Val Ala Val Pro Val Ile Val His Arg Glu

100 105 110

Ile Arg Gly Val Leu Tyr Val Gly Val His Ser Ala Val Arg Leu Gly

115 120 125

Asp Thr Val Ile Glu Glu Val Thr Met Thr Ala Arg Thr Leu Glu Gln

130 135 140

Asn Leu Ala Ile Asn Ser Ala Leu Arg Arg Asn Gly Val Pro Asp Gly

145 150 155 160

Arg Gly Ser Leu Lys Ala Asn Arg Val Met Asn Gly Ala Glu Trp Glu

165 170 175

Gln Val Arg Ser Thr His Ser Lys Leu Arg Met Leu Ala Asn Arg Val

180 185 190

Thr Asp Glu Asp Leu Arg Arg Asp Leu Glu Glu Leu Cys Asp Gln Met

195 200 205

Val Thr Pro Val Arg Ile Lys Gln Thr Thr Lys Leu Ser Ala Arg Glu

210 215 220

Leu Asp Val Leu Ala Cys Val Ala Leu Gly His Thr Asn Val Glu Ala

225 230 235 240

Ala Glu Glu Met Gly Ile Gly Ala Glu Thr Val Lys Ser Tyr Leu Arg

245 250 255

Ser Val Met Arg Lys Leu Gly Ala His Thr Arg Tyr Glu Ala Val Asn

260 265 270

Ala Ala Arg Arg Ile Gly Ala Leu Pro

275 280

<210>71

<211>478

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(448)

<223>RXA06017

<400>71

cgatcacccc ttcgccggcc gccaagactt agaactatcc gccttagaag acctcgatct 60

gctgcttctc gacgacggac actgcctcca cgaccaaatt gtg gac ctg tgc cgc 115

Val Asp Leu Cys Arg

1 5

cgc gga gac atc aac ccc att agc tcc act act gct gtc acc cgc gca 163

Arg Gly Asp Ile Asn Pro Ile Ser Ser Thr Thr Ala Val Thr Arg Ala

10 15 20

tcc agc ctt acc acc gtc atg cag ctc gtc gtc gcc ggc ctt gga tcc 211

Ser Ser Leu Thr Thr Val Met Gln Leu Val Val Ala Gly Leu Gly Ser

25 30 35

acc ttg gtc cca atc agc gca atc cca tgg gaa tgc acc cga cca gga 259

Thr Leu Val Pro Ile Ser Ala Ile Pro Trp Glu Cys Thr Arg Pro Gly

40 45 50

ctg gca aca gcc aac ttc aac tct gat gtc acc gca aac cgc cgc att 307

Leu Ala Thr Ala Asn Phe Asn Ser Asp Val Thr Ala Asn Arg Arg Ile

55 60 65

gga ttg gtg tac cgt tcc tct tct tct cgc gcc gaa gag ttc gaa cag 355

Gly Leu Val Tyr Arg Ser Ser Ser Ser Arg Ala Glu Glu Phe Glu Gln

70 75 80 85

ttt gca ctc att ttg cag cgc gct ttc caa gaa gcc gtc gcg ctt gct 403

Phe Ala Leu Ile Leu Gln Arg Ala Phe Gln Glu Ala Val Ala Leu Ala

90 95 100

gcc tca act ggc atc acc ttg aag caa aat gtc gcg gta gcg cag 448

Ala Ser Thr Gly Ile Thr Leu Lys Gln Asn Val Ala Val Ala Gln

105 110 115

taagtttttc tagaggtttt ccagagtcag 478

<210>72

<211>116

<212>PRT

<213>谷氨酸棒状杆菌

<400>72

Val Asp Leu Cys Arg Arg Gly Asp Ile Asn Pro Ile Ser Ser Thr Thr

1 5 10 15

Ala Val Thr Arg Ala Ser Ser Leu Thr Thr Val Met Gln Leu Val Val

20 25 30

Ala Gly Leu Gly Ser Thr Leu Val Pro Ile Ser Ala Ile Pro Trp Glu

35 40 45

Cys Thr Arg Pro Gly Leu Ala Thr Ala Asn Phe Asn Ser Asp Val Thr

50 55 60

Ala Asn Arg Arg Ile Gly Leu Val Tyr Arg Ser Ser Ser Ser Arg Ala

65 70 75 80

Glu Glu Phe Glu Gln Phe Ala Leu Ile Leu Gln Arg Ala Phe Gln Glu

85 90 95

Ala Val Ala Leu Ala Ala Ser Thr Gly Ile Thr Leu Lys Gln Asn Val

100 105 110

Ala Val Ala Gln

115

<210>73

<211>1197

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(1)..(1197)

<223>RXA07002

<400>73

atg aat cac aga ccc ggc ctg acc ttc cgc ttc ctg gcc gcc cag gtg 48

Met Asn His Arg Pro Gly Leu Thr Phe Arg Phe Leu Ala Ala Gln Val

1 5 10 15

ttg gtc gtg gtg att agc ctg ctg gtg gcc gcg gcc gtg gcc acg atg 96

Leu Val Val Val Ile Ser Leu Leu Val Ala Ala Ala Val Ala Thr Met

20 25 30

gtg ggc ccg acc ctg ttc cat gat cat atg ttg atg acc ggc cgg gag 144

Val Gly Pro Thr Leu Phe His Asp His Met Leu Met Thr Gly Arg Glu

35 40 45

gac ccc tcg ctg gag ctg ttc cat gcc gag cag gcc tac cgg gac gcc 192

Asp Pro Ser Leu Glu Leu Phe His Ala Glu Gln Ala Tyr Arg Asp Ala

50 55 60

aac ctg atc acc ctg gcc gtc gcc ctg ccc acc gcc ttg atc agc gcc 240

Asn Leu Ile Thr Leu Ala Val Ala Leu Pro Thr Ala Leu Ile Ser Ala

65 70 75 80

ctg ctg gcc agc ctg tgg tta tcg cgt cgc ctg cgc acc ccc ctg cag 288

Leu Leu Ala Ser Leu Trp Leu Ser Arg Arg Leu Arg Thr Pro Leu Gln

85 90 95

gat ctc acc cgc gcc gct acc agc ctg acg gcc ggc aac tac cgt atc 336

Asp Leu Thr Arg Ala Ala Thr Ser Leu Thr Ala Gly Asn Tyr Arg Ile

100 105 110

cgc gtg ccc gcc gga gaa gca ggc ccc gag gtc acc acc ctg gcg cat 384

Arg Val Pro Ala Gly Glu Ala Gly Pro Glu Val Thr Thr Leu Ala His

115 120 125

gcc ttc aac acc atg gcc gac cgg ctg gaa cac acc gaa cag gtc cgc 432

Ala Phe Asn Thr Met Ala Asp Arg Leu Glu His Thr Glu Gln Val Arg

130 135 140

cgc cag atg ctc tct gat ctg gcc cac gaa atg ggc acc ccc tta tcg 480

Arg Gln Met Leu Ser Asp Leu Ala His Glu Met Gly Thr Pro Leu Ser

145 150 155 160

gtg ctc acg gtc tac ctc gat ggt ctc cag gac ggg gtc gtg gac tgg 528

Val Leu Thr Val Tyr Leu Asp Gly Leu Gln Asp Gly Val Val Asp Trp

165 170 175

aat aat gcc acc cac acg atc atg gct gac caa ctc acc cgc ctg acc 576

Asn Asn Ala Thr His Thr Ile Met Ala Asp Gln Leu Thr Arg Leu Thr

180 185 190

cgg ttg atg gaa gac atc gac gat gtc tcc cgg gcc cag gaa cac cgg 624

Arg Leu Met Glu Asp Ile Asp Asp Val Ser Arg Ala Gln Glu His Arg

195 200 205

atc gat ttg gac ctg gcg gag gaa ggg ctc ggg gat ctg ctc cat acc 672

Ile Asp Leu Asp Leu Ala Glu Glu Gly Leu Gly Asp Leu Leu His Thr

210 215 220

gcc gct gct gcc gcg ggg gaa gct tat gct gac aaa ggc gtc gat tta 720

Ala Ala Ala Ala Ala Gly Glu Ala Tyr Ala Asp Lys Gly Val Asp Leu

225 230 235 240

cag gtc gag acc att atg gac acc gtc cgg gtg ctc gtg gac cgg caa 768

Gln Val Glu Thr Ile Met Asp Thr Val Arg Val Leu Val Asp Arg Gln

245 250 255

cgc ttc ggc cag gtg atg agc aat ctc ctg tcg aac gcg cta cgg tac 816

Arg Phe Gly Gln Val Met Ser Asn Leu Leu Ser Asn Ala Leu Arg Tyr

260 265 270

acc ccg gcc ggc ggg cag gtc cgg atc agc gtc cac cga cag ggg gcg 864

Thr Pro Ala Gly Gly Gln Val Arg Ile Ser Val His Arg Gln Gly Ala

275 280 285

tcc acc gcg ctc atc cac gtc gcc gat gac ggc gag ggc atc cca cct 912

Ser Thr Ala Leu Ile His Val Ala Asp Asp Gly Glu Gly Ile Pro Pro

290 295 300

ggc cag ctc gga cac atc ttc gaa cgc ttc tac cgg ggg gat gcc gcc 960

Gly Gln Leu Gly His Ile Phe Glu Arg Phe Tyr Arg Gly Asp Ala Ala

305 310 315 320

cgc agc cgg gac aac ggc ggg gcc ggt atc ggt ctg acc atc tcc aag 1008

Arg Ser Arg Asp Asn Gly Gly Ala Gly Ile Gly Leu Thr Ile Ser Lys

325 330 335

gca ttg atc gag gcc cac ggc ggc act ctc acc gcc acc tct cct ggc 1056

Ala Leu Ile Glu Ala His Gly Gly Thr Leu Thr Ala Thr Ser Pro Gly

340 345 350

ccc ggt gcc gga tcg gtc ttc acc atc cgt ctt ccc ctg cac cag gaa 1104

Pro Gly Ala Gly Ser Val Phe Thr Ile Arg Leu Pro Leu His Gln Glu

355 360 365

aac gtg tcc ctg atg ctc agt gac ccc act ccc ggg gac aat aat tct 1152

Asn Val Ser Leu Met Leu Ser Asp Pro Thr Pro Gly Asp Asn Asn Ser

370 375 380

gat gac ctc ggc agc gat ccg tat caa ccc ctt gac aat acc cca 1197

Asp Asp Leu Gly Ser Asp Pro Tyr Gln Pro Leu Asp Asn Thr Pro

385 390 395

<210>74

<211>399

<212>PRT

<213>谷氨酸棒状杆菌

<400>74

Met Asn His Arg Pro Gly Leu Thr Phe Arg Phe Leu Ala Ala Gln Val

1 5 10 15

Leu Val Val Val Ile Ser Leu Leu Val Ala Ala Ala Val Ala Thr Met

20 25 30

Val Gly Pro Thr Leu Phe His Asp His Met Leu Met Thr Gly Arg Glu

35 40 45

Asp Pro Ser Leu Glu Leu Phe His Ala Glu Gln Ala Tyr Arg Asp Ala

50 55 60

Asn Leu Ile Thr Leu Ala Val Ala Leu Pro Thr Ala Leu Ile Ser Ala

65 70 75 80

Leu Leu Ala Ser Leu Trp Leu Ser Arg Arg Leu Arg Thr Pro Leu Gln

85 90 95

Asp Leu Thr Arg Ala Ala Thr Ser Leu Thr Ala Gly Asn Tyr Arg Ile

100 105 110

Arg Val Pro Ala Gly Glu Ala Gly Pro Glu Val Thr Thr Leu Ala His

115 120 125

Ala Phe Asn Thr Met Ala Asp Arg Leu Glu His Thr Glu Gln Val Arg

130 135 140

Arg Gln Met Leu Ser Asp Leu Ala His Glu Met Gly Thr Pro Leu Ser

145 150 155 160

Val Leu Thr Val Tyr Leu Asp Gly Leu Gln Asp Gly Val Val Asp Trp

165 170 175

Asn Asn Ala Thr His Thr Ile Met Ala Asp Gln Leu Thr Arg Leu Thr

180 185 190

Arg Leu Met Glu Asp Ile Asp Asp Val Ser Arg Ala Gln Glu His Arg

195 200 205

Ile Asp Leu Asp Leu Ala Glu Glu Gly Leu Gly Asp Leu Leu His Thr

210 215 220

Ala Ala Ala Ala Ala Gly Glu Ala Tyr Ala Asp Lys Gly Val Asp Leu

225 230 235 240

Gln Val Glu Thr Ile Met Asp Thr Val Arg Val Leu Val Asp Arg Gln

245 250 255

Arg Phe Gly Gln Val Met Ser Asn Leu Leu Ser Asn Ala Leu Arg Tyr

260 265 270

Thr Pro Ala Gly Gly Gln Val Arg Ile Ser Val His Arg Gln Gly Ala

275 280 285

Ser Thr Ala Leu Ile His Val Ala Asp Asp Gly Glu Gly Ile Pro Pro

290 295 300

Gly Gln Leu Gly His Ile Phe Glu Arg Phe Tyr Arg Gly Asp Ala Ala

305 310 315 320

Arg Ser Arg Asp Asn Gly Gly Ala Gly Ile Gly Leu Thr Ile Ser Lys

325 330 335

Ala Leu Ile Glu Ala His Gly Gly Thr Leu Thr Ala Thr Ser Pro Gly

340 345 350

Pro Gly Ala Gly Ser Val Phe Thr Ile Arg Leu Pro Leu His Gln Glu

355 360 365

Asn Val Ser Leu Met Leu Ser Asp Pro Thr Pro Gly Asp Asn Asn Ser

370 375 380

Asp Asp Leu Gly Ser Asp Pro Tyr Gln Pro Leu Asp Asn Thr Pro

385 390 395

<210>75

<211>951

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(1)..(951)

<223>RXA07003

<400>75

gtg gcg gta gct ggg ctg agt gca agc gtt gag agc aaa atc gac acc 48

Val Ala Val Ala Gly Leu Ser Ala Ser Val Glu Ser Lys Ile Asp Thr

1 5 10 15

atc cgg gac acg ttg aag gaa act gcg tgc gca atc gca aac atg ccg 96

Ile Arg Asp Thr Leu Lys Glu Thr Ala Cys Ala Ile Ala Asn Met Pro

20 25 30

ccagcc aaa acg cag gaa ttc gat cca tca cga atc aag gaa ccc aat 144

Pro Ala Lys Thr Gln Glu Phe Asp Pro Ser Arg Ile Lys Glu Pro Asn

35 40 45

tca cct tcg gta atc acc aaa gcg gca acg ctc atg gat gtg ctc cgc 192

Ser Pro Ser Val Ile Thr Lys Ala Ala Thr Leu Met Asp Val Leu Arg

50 55 60

act gaa ggt ccc acc aac tcc gct cgc cta gct gag gtg ctg ggg gag 240

Thr Glu Gly Pro Thr Asn Ser Ala Arg Leu Ala Glu Val Leu Gly Glu

65 70 75 80

ccg atc agt tcg gtg tac aga atg ctc cac acc tta acc gcg atc ggg 288

Pro Ile Ser Ser Val Tyr Arg Met Leu His Thr Leu Thr Ala Ile Gly

85 90 95

tgg gtt gag cag gac gga aag cgc ggc tcc tat cgc gtt ggc cta gcc 336

Trp Val Glu Gln Asp Gly Lys Arg Gly Ser Tyr Arg Val Gly Leu Ala

100 105 110

atg ctc aca ctt gcc gaa ttg caa ttg cgc cat atg gat ctc cgc aag 384

Met Leu Thr Leu Ala Glu Leu Gln Leu Arg His Met Asp Leu Arg Lys

115 120 125

atc gcc gca cta aca atg cgg aaa att cat gca ctc act ggt gaa acc 432

Ile Ala Ala Leu Thr Met Arg Lys Ile His Ala Leu Thr Gly Glu Thr

130 135 140

aca ttt tta tgt gtt cgt cac ggc att cgc gcg gta tgc atc gag agg 480

Thr Phe Leu Cys Val Arg His Gly Ile Arg Ala Val Cys Ile Glu Arg

145 150 155 160

gtc gat ggc gat cgt gtg aat agt cga gtt ctc cag ctg gga acc tcg 528

Val Asp Gly Asp Arg Val Asn Ser Arg Val Leu Gln Leu Gly Thr Ser

165 170 175

ctg ccg ctc cat gtc ggt gcc gca cct cgt gcg ctt ctc gct ttt gag 576

Leu Pro Leu His Val Gly Ala Ala Pro Arg Ala Leu Leu Ala Phe Glu

180 185 190

gga cgc agg gcc tgg gaa aca tat gcc aca aac tta gga ttc gag ggc 624

Gly Arg Arg Ala Trp Glu Thr Tyr Ala Thr Asn Leu Gly Phe Glu Gly

195 200 205

cat aac tgg tct aaa gga ccg tcc aga ggc gag cta ttc caa cac ttg 672

His Asn Trp Ser Lys Gly Pro Ser Arg Gly Glu Leu Phe Gln His Leu

210 215 220

gac gaa gac cgc gat aag ggt ttt tgc ctg gta gac aat gaa att act 720

Asp Glu Asp Arg Asp Lys Gly Phe Cys Leu Val Asp Asn Glu Ile Thr

225 230 235 240

ccc ggg atc gcc gct gta gga gca ccg att tac aac cat cgt ggt gaa 768

Pro Gly Ile Ala Ala Val Gly Ala Pro Ile Tyr Asn His Arg Gly Glu

245 250 255

gtc gtg gca agc ctg tcc atg agt gga ctg cgg gac ggc atc ctc agc 816

Val Val Ala Ser Leu Ser Met Ser Gly Leu Arg Asp Gly Ile Leu Ser

260 265 270

gat acc acc gac tac tcg gcc gtg gaa ctg atc ctg cag ggg tct gcg 864

Asp Thr Thr Asp Tyr Ser Ala Val Glu Leu Ile Leu Gln Gly Ser Ala

275 280 285

gag att tcc caa gcg ctg gga gcg acc att gag cac aac ggg ggc aac 912

Glu Ile Ser Gln Ala Leu Gly Ala Thr Ile Glu His Asn Gly Gly Asn

290 295 300

caa aaa cta ccc cag gta acc cct ctg agc att gtg gtt 951

Gln Lys Leu Pro Gln Val Thr Pro Leu Ser Ile Val Val

305 310 315

<210>76

<211>317

<212>PRT

<213>谷氨酸棒状杆菌

<400>76

Val Ala Val Ala Gly Leu Ser Ala Ser Val Glu Ser Lys Ile Asp Thr

1 5 10 15

Ile Arg Asp Thr Leu Lys Glu Thr Ala Cys Ala Ile Ala Asn Met Pro

20 25 30

Pro Ala Lys Thr Gln Glu Phe Asp Pro Ser Arg Ile Lys Glu Pro Asn

35 40 45

Ser Pro Ser Val Ile Thr Lys Ala Ala Thr Leu Met Asp Val Leu Arg

50 55 60

Thr Glu Gly Pro Thr Asn Ser Ala Arg Leu Ala Glu Val Leu Gly Glu

65 70 75 80

Pro Ile Ser Ser Val Tyr Arg Met Leu His Thr Leu Thr Ala Ile Gly

85 90 95

Trp Val Glu Gln Asp Gly Lys Arg Gly Ser Tyr Arg Val Gly Leu Ala

100 105 110

Met Leu Thr Leu Ala Glu Leu Gln Leu Arg His Met Asp Leu Arg Lys

115 120 125

Ile Ala Ala Leu Thr Met Arg Lys Ile His Ala Leu Thr Gly Glu Thr

130 135 140

Thr Phe Leu Cys Val Arg His Gly Ile Arg Ala Val Cys Ile Glu Arg

145 150 155 160

Val Asp Gly Asp Arg Val Asn Ser Arg Val Leu Gln Leu Gly Thr Ser

165 170 175

Leu Pro Leu His Val Gly Ala Ala Pro Arg Ala Leu Leu Ala Phe Glu

180 185 190

Gly Arg Arg Ala Trp Glu Thr Tyr Ala Thr Asn Leu Gly Phe Glu Gly

195 200 205

His Asn Trp Ser Lys Gly Pro Ser Arg Gly Glu Leu Phe Gln His Leu

210 215 220

Asp Glu Asp Arg Asp Lys Gly Phe Cys Leu Val Asp Asn Glu Ile Thr

225 230 235 240

Pro Gly Ile Ala Ala Val Gly Ala Pro Ile Tyr Asn His Arg Gly Glu

245 250 255

Val Val Ala Ser Leu Ser Met Ser Gly Leu Arg Asp Gly Ile Leu Ser

260 265 270

Asp Thr Thr Asp Tyr Ser Ala Val Glu Leu Ile Leu Gln Gly Ser Ala

275 280 285

Glu Ile Ser Gln Ala Leu Gly Ala Thr Ile Glu His Asn Gly Gly Asn

290 295 300

Gln Lys Leu Pro Gln Val Thr Pro Leu Ser Ile Val Val

305 310 315

Claims

1.分离的核酸分子，其编码的多肽所具有的氨基酸序列在每种情况下均涉及表1/第2列，其中在表1/第4列所示氨基酸位置处的核酸分子所编码的蛋白原氨基酸与表1/第5列相同行所示的特定氨基酸不同。

2.如权利要求1所述的分离的核酸分子，其中在表1/第4列中所示氨基酸位置处的核酸分子编码表1/第6列相同行所示的氨基酸。

3.载体，其包含至少一个如权利要求1所述的核酸序列。

4.宿主细胞，其用至少一种如权利要求3所述的载体转染。

5.如权利要求4所述的宿主细胞，其中所述核酸分子的表达调节所述细胞精细化学品的生产率。

6.制备精细化学品的方法，其包括培养已用至少一种如权利要求3所述的载体转染的细胞，从而产生精细化学品。

7.如权利要求6所述的方法，其中精细化学品是氨基酸。

8.如权利要求7所述的方法，其中所述氨基酸是赖氨酸。