CN1578834A

CN1578834A - 编码代谢途径蛋白的基因

Info

Publication number: CN1578834A
Application number: CNA028216520A
Authority: CN
Inventors: O·策尔德尔; M·蓬佩尤斯; H·施罗德; B·克勒格尔; C·克洛普罗格; G·哈伯豪尔
Original assignee: BASF SE
Current assignee: BASF SE
Priority date: 2001-11-05
Filing date: 2002-10-31
Publication date: 2005-02-09
Also published as: ZA200404426B; EP1444343A2; WO2003040681A3; AU2002346807A1; US20070072274A1; BR0213774A; US7141663B2; US7355032B2; US20050019877A1; KR20050042245A; KR100868693B1; WO2003040681A2; DE10154292A1

Abstract

本发明涉及新的核酸分子，涉及其用于构建遗传改良的微生物的用途，还涉及借助于所述遗传改良的微生物制备精细化学品的方法，尤其是制备氨基酸的方法。

Description

编码代谢途径蛋白的基因

发明背景

细胞中天然进行的代谢过程所产生的特定产物和副产物已用于工业的许多分支中，包括食品工业、动物饲料工业、化妆品工业及药学工业。这些分子合起来称为“精细化学品”，其包括有机酸(蛋白原的和非蛋白原的氨基酸)、核苷酸和核苷、脂类和脂肪酸、二醇、碳水化合物、芳香化合物、维生素和辅因子、以及酶。它们最好的产生是通过大规模培养细菌，使产生并分泌大量的一种或多种目标分子。一种对此目的尤其有用的生物体是谷氨酸棒状杆菌(Corynebacterium glutamicum)，其为革兰氏阳性的非致病菌。通过菌株选择，已开发了许多突变菌株，它们产生多种目标化合物。但是，为提高特定分子产量的菌株选择是一种费时且困难的方法。

发明简述

本发明提供了新的可用于谷氨酸棒状杆菌或相关菌种的鉴定或分类的核酸分子。谷氨酸棒状杆菌为革兰氏阳性需氧细菌，其广泛用在工业上用于大规模产生多种精细化学品，也用于降解碳水化合物(如在原油泄漏物中)，以及用于氧化萜类化合物。本发明的核酸分子因此可用于鉴定能如通过发酵法用来产生精细化学品的微生物。虽然谷氨酸棒状杆菌自身为非致病的，但它与其它棒状杆菌如对人致病的重要病原菌白喉棒状杆菌(Corynebacterium diphtheriae)(白喉的病原菌)有亲缘关系。所以鉴定存在棒状杆菌的能力也具有显著的临床重要性，例如诊断应用。而且，所述核酸分子可作为对谷氨酸棒状杆菌或相关生物进行基因组作图的参考点。

这些新核酸分子编码的蛋白质文中称为代谢途径(MP)蛋白。这些MP蛋白可以例如发挥参与对细胞的正常代谢功能起关键作用的蛋白的转录、翻译或翻译后调节的功能。由于可得到用于谷氨酸棒状杆菌中的克隆载体(如在Sinskey等人，美国专利号4,649,119中所公开的)和用于遗传操作谷氨酸棒状杆菌和相关短杆菌种(如lactofermentum)的技术(Yoshihama等人，细菌学杂志(J.Bacteriol.)162：(1985)591-597；Katsumata等人，细菌学杂志(J.Bacteriol.)159：(1984)306-311；和Santamaria等人，普通微生物学杂志(J.Gen.Microbiol.)130：(1984)2237-2246)，本发明的核酸分子可用于该生物体的遗传操作，使其成为一种或多种精细化学品的较好或更有效的生产者。

通过操作本发明的基因可以直接或间接地导致精细化学品产量、生产率和/或生产效率的提高。更特别地，对通常调节精细化学品代谢途径中精细化学品的产量、生产率和/或生产效率的谷氨酸棒状杆菌MP蛋白的修饰可能对所述生物体的一种或多种这些所需化合物的总生产率或生产速率有直接的影响。对参与这些代谢途径的蛋白的修饰可能也对目的精细化学品的产量、生产率和/或生产效率有间接影响。代谢调节不可避免地是复杂的并且影响不同途径的调节机制在许多地方可重叠以致可以根据特定细胞事件快速调节两种以上的代谢途径。这使得可修饰一种代谢途径中的调节蛋白也可影响许多其他代谢途径，它们中的一些可能参与目的精细化学品的生物合成或降解。在这种间接方式中，对MP蛋白的作用进行调节可能影响精细化学品的生产率，所述该精细化学品产生的代谢途径不同于受所述MP蛋白直接调节的代谢途径。

可以使用本发明的核酸和蛋白分子以便直接提高谷氨酸棒状杆菌的一种或多种目的精细化学品的产量、生产率和/或生产效率。可通过本领域已知的基因重组技术操作一种或多种本发明调节蛋白，从而使得它们的功能被调节。参与抑制编码氨基酸生物合成所需的酶的基因转录使得所述氨基酸不再能够抑制该转录的MP蛋白的突变可导致例如，所述氨基酸生产率的提高。所以，MP蛋白活性的修饰(其导致参与目的精细化学品生物合成的谷氨酸棒状杆菌蛋白的翻译增强或翻译后修饰的活化)接下来可增加所述化学品的生产率。相反的情况可同样有用：通过增强对转录或翻译的抑制或者通过对参与调节化合物降解途径的谷氨酸棒状杆菌蛋白的翻译后负修饰，可增加所述化学品的生产率。在任何一种情况下，可以增加该目的精细化学品的总产量或生产率。

同样，本发明蛋白和核苷酸分子中的所述修饰可通过间接机制提高精细化学品的产量、生产率和/或生产效率。特定化合物的代谢不可避免地与细胞中其他生物合成和降解途径相关，并且代谢途径中的必需辅因子、中间物或底物都可能被另一个代谢途径所提供或限制。所以调节本发明的一种或多种调节蛋白能够影响精细化学品的其他生物合成或降解途径的活性的效率。除此之外，对一种或多种调节蛋白的操作可增加细胞在培养物中，特别是在大规模发酵培养物(其中的生长条件可能不是最优的)中的生长和增殖的总体能力。例如通过突变通常抑制核苷生物合成的本发明MP蛋白，使得所述蛋白具有较低的抑制活性，从而可增加核苷的生物合成和细胞分裂，其中所述核苷生物合成的抑制为对营养的非最优胞外提供而产生的反应(从而阻止细胞分裂)。在那些导致培养物中细胞生长和分裂增加的MP蛋白的修饰可以导致培养物中一种或多种目标精细化学品的产量、生产率和/或生产效率增加，这至少是因为培养物中生产所述化学品的细胞数目的增加。

本发明提供了编码本文中称作代谢调节(MP)蛋白的新核酸分子并且所述蛋白例如能够进行和谷氨酸棒状杆菌中代谢途径的转录、翻译或翻译后调节相关的酶促步骤。本文中编码MP蛋白的核酸分子被称作MP核酸分子。在一个优选的实施方案中，MP蛋白参与一个或多个代谢途径的转录、翻译或翻译后调节。这些蛋白的实例是表1中列出的基因编码的蛋白。

因此，本发明的一个方面涉及这样的分离核酸分子(例如cDNA)，其包含编码MP蛋白或其生物学活性部分的核苷酸序列和适合作为检测或扩增编码MP的核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在尤其优选的实施方案中，该分离的核酸分子包含任何附录A所列出的核苷酸序列或任何这些核苷酸序列的编码区或其互补序列。在其他优选的实施方案中，该分离的核酸分子编码附录B中列出的任何氨基酸序列。本发明优选的MP蛋白同样优选地具有至少一种本文所描述的MP活性。

附录A定义了下面的表1中描述的序列表的核酸序列以及相关位置的序列修饰。

附录B定义了下面的表1中描述的序列表的多肽序列以及相关位置的序列修饰。

在另一个实施方案中，分离的核酸分子长度至少为15个核苷酸并且在严紧条件下与含有附录A的核苷酸序列的核酸分子杂交。分离的核酸分子优选与天然存在的核酸分子对应。分离的核酸更优选编码天然存在的谷氨酸棒状杆菌MP蛋白或其生物活性部分。

本发明的另一个方面涉及包含本发明核酸分子的载体(例如重组表达载体)，还涉及已导入所述载体的宿主细胞。在一个实施方案中，通过使用在合适的培养基中培养的宿主细胞制备了MP蛋白。然后可从培养基或宿主细胞中分离该MP蛋白。

本发明的另一个方面涉及已导入MP基因的或其中MP基因被修饰的经遗传修饰的微生物。在一个实施方案中，通过导入至少一种作为转基因的编码突变MP序列的本发明核酸分子而修饰了所述微生物的基因组。在另一个实施方案中，通过用经修饰的MP基因进行同源重组而修饰(例如功能性破坏)了所述微生物基因组中的内源性MP基因。在一个优选的实施方案中，微生物属于棒状杆菌属或短杆菌(Brevibacterium)属，尤其优选谷氨酸棒状杆菌。在一个优选的实施方案中，该微生物也被用于制备目标化合物，如氨基酸、尤其是赖氨酸。

另一个优选的实施方案是具有一种或多种附录A中所描述核酸分子的宿主细胞。可用技术人员已知的多种方法制备这些宿主细胞。例如，可通过携带几种本发明核酸分子的载体转染这些细胞。然而，也可使用每次导入一种本发明核酸分子的载体从而同时或相继使用多种载体。从而可构建携带许多、高达数百的本发明核酸序列的宿主细胞。这种积累通常能够对宿主细胞精细化学品的生产率产生超加性效果。

本发明的另一方面涉及分离的MP蛋白或其部分，例如其生物活性部分。在一个优选的实施方案中，分离的MP蛋白或其部分在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的一种或多种代谢途径。在另一个优选的实施方案中，分离的MP蛋白和其部分与附录B的氨基酸序列足够同源，因为该蛋白或其部分仍然能够转录地、翻译地或翻译后地调节谷氨酸棒状杆菌中的一种或多种代谢途径。

此外，本发明涉及分离的MP蛋白的制备。在优选的实施方案中，该MP蛋白含有附录B的氨基酸序列。在另一个优选的实施方案中，本发明涉及分离的全长蛋白，其和附录B的全长氨基酸序列(其由附录A中的可读框编码)充分同源。

可以将MP多肽或其生物活性部分与非MP多肽功能性地连接以产生融合蛋白。在优选的实施方案中，该融合蛋白和单独的MP蛋白具有不同的活性，在其它优选的实施方案中，该融合蛋白转录地、翻译地或翻译后地调节谷氨酸棒状杆菌的一种或多种代谢途径。在尤其优选的实施方案中，所述融合蛋白向宿主细胞的整合调节细胞对目的化合物的生产率。

本发明的另一个方面涉及制备精细化学品的制备方法。该方法涉及含有载体的细胞的培养，这导致本发明MP核酸分子的表达而产生精细化学品。在一个优选的实施方案中，该方法还包括得到含有此类载体的细胞的步骤，所述细胞用载体转染导致MP核酸表达。在另一个优选的实施方案中，所述方法还包括从培养物中得到精细化学品的步骤。在一个优选的实施方案中，该细胞属于棒状杆菌属或短杆菌属。

本发明的另一个方面涉及调节来自微生物的分子生产率的方法。这些方法包括将细胞和调节MP蛋白活性或MP核酸表达的物质接触，使得和没有所述物质存在时相比细胞相关的活性受到调节。在一个优选的实施方案中，细胞关于谷氨酸棒状杆菌中代谢途径的一种或多种调节系统受到调节，使得该微生物的目标精细化学品的产量或生产率提高。调节MP蛋白活性的物质刺激例如MP蛋白的活性或MP核酸的表达。刺激MP蛋白活性或MP核酸表达的物质的例子包括小分子、活性MP蛋白和编码MP蛋白并已导入细胞的核酸分子。抑制MP活性或MP表达的物质的例子包括小分子和反义MP核酸分子。

本发明的另一个方面涉及调节细胞的目的化合物产量的方法，该方法包括向细胞中导入MP野生型基因或MP突变基因，该基因仍位于分立的质粒上或者整合到宿主细胞的基因组中。向基因组的整合可随机发生或者通过同源重组发生，由此天然基因被整合的拷贝所代替，导致细胞的目标化合物的生产率被调节。在一个优选的实施方案中，所述产量增加了。在另一个优选的实施方案中，化学品是精细化学品，其在一个特别优选的实施方案中是氨基酸。在一个特别优选的实施方案中，该氨基酸是L-赖氨酸。

发明详述

本发明提供了参与谷氨酸棒状杆菌代谢调节，包括参与精细化学品代谢调节的MP核酸和MP蛋白分子。本发明的这些分子可用于直接调节(例如当对赖氨酸代谢途径的调节蛋白活性的调节对该微生物的赖氨酸产量、生产率和/或生产效率具有直接影响)或间接调节微生物如谷氨酸棒状杆菌的精细化学品的生产率，间接调节仍然增加目标化合物的产量、生产率和/或生产效率(例如，当对核苷酸生物合成蛋白的调节对细菌的有机酸或脂肪酸的生产率具有影响时，这可能是由于所述化学品的生物合成或降解途径中伴随的调节修饰对核苷酸生物合成的修饰调节的一种反应)。本发明的诸方面进一步阐述如下。

I.精细化学品

术语“精细化学品”为本领域所认知，其包括已用于多种工业的由生物体产生的分子，其中所述工业例如但不限于药学工业、农业工业和化妆品工业。此类化合物包括有机酸如酒石酸、衣康酸和二氨基庚二酸、蛋白原的和非蛋白原的氨基酸、嘌呤和嘧啶碱基、核苷和核苷酸(如在Rehm等人编辑的生物技术(Biotechnology)第6卷中Kuninaka，A.(1996)核苷酸和相关化合物(Nucleotides and related compounds)，561-612页，VCH：Weinheim及其中所含参考文献中所述)、脂类、饱和和不饱和脂肪酸(如花生四烯酸)、二醇(如丙二醇和丁二醇)、碳水化合物(如透明质酸和海藻糖)、芳香化合物(如芳香胺、香草醛和靛蓝)、维生素和辅因子(如在Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)，A27卷，“维生素”(“Vitamins”)，443-613页(1996)VCH：Weinheim及其中的参考文献；以及Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition，Lipids，Health，and Disease”Proceedings ofthe UNESCO/Confederation of Scientific and Technological Associationsin Malaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press，(1995))、酶以及在Gutcho(1983)通过发酵的化学品(Chemicals by Fermentation)，Noyes DataCorporation，ISBN：0818805086和其中的参考文献中所述的所有其它化学品。这些精细化学品中的某些化学品的代谢和用途进一步阐述如下。

A.氨基酸代谢和用途

氨基酸包含所有蛋白质的基本结构单元，并且为所有生物体的正常细胞功能所必需。术语“氨基酸”为本领域所认知。蛋白原的氨基酸有20种，为蛋白质的结构单元，在蛋白质中它们通过肽键连接，非蛋白原的氨基酸(已知成百种非蛋白原的氨基酸)在蛋白质中通常不存在(参见Ulmann工业化学百科全书(Ulmann工业化学百科全书(Ulmann’s Encyclopediaof Industrial Chemistry))，A2卷，57-97页，VCH：Weinheim(1985))。氨基酸可为D-或L-构型，尽管在天然存在的蛋白质中发现的唯一类型通常为L-氨基酸。已充分说明了20种蛋白原氨基酸中每一种在原核和真核细胞中的生物合成和降解途径(参见例如，Stryer，L.生物化学(Biochemistry)，第三版，578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)，这样称呼是因为由于它们生物合成的复杂性，必须从食物中摄取它们，它们被简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物能够合成这些氨基酸中的一部分氨基酸，但必需氨基酸必须从食物中摄取，以进行正常的蛋白质合成。

除了它们在蛋白质生物合成中的功能外，这些氨基酸本身还是令人感兴趣的化学品，并且许多已发现在食品、动物饲料、化学品、化妆品、农业和药学工业中具有多种应用。赖氨酸不仅对人的营养而且对单胃动物如禽和猪都是一种重要的氨基酸。谷氨酸盐是最常用的调味添加剂(谷氨酸单钠盐，MSG)，并且广泛用于食品工业，天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸均用于药学工业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸用于药学工业和化妆品工业。苏氨酸、色氨酸和D/L-甲硫氨酸是广泛使用的动物饲料添加剂。(Rehm等人(编辑)，生物技术(Biotechnology)，第6卷，第14a章中的Leuchtenberger，W.(1996)Amino aids-technicalproduction and use，466-502页，VCH：Weinheim)。还发现这些氨基酸可用作前体，用于合成合成的氨基酸和蛋白质，如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟色氨以及在其他Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)，A2卷，57-97页，VCH：Weinheim，1985中所述的物质。

在可产生它们的生物体如细菌中已充分描述了这些天然氨基酸的生物合成(细菌氨基酸生物合成及其调节的综述，见Umbarger，H.E.(1978)Ann.Rev.Biochem.47：533-606)。谷氨酸通过还原胺化α-酮戊二酸(柠檬酸循环中的中间体)而合成。谷氨酰胺、脯氨酸和精氨酸接下来由谷氨酸产生。丝氨酸的生物合成为三步，由3-磷酸甘油(糖酵解中间体)开始，在氧化、氨基转移和水解步骤后产生该氨基酸。半胱氨酸和甘氨酸两者均产生自丝氨酸；在丝氨酸转羟甲基酶催化的反应中前者通过高半胱氨酸与丝氨酸缩合，后者通过侧链β-碳原子转移至四氢叶酸。苯丙氨酸和酪氨酸在9步的生物合成途径中合成自糖分解和磷酸戊糖途径的前体赤藓糖4-磷酸和磷酸烯醇丙酮酸，它们的不同仅在预苯酸合成后的最后两步。色氨酸也从这两种起始分子产生，但其合成为11步的途径。酪氨酸也可在苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸均为丙酮酸盐(糖酵解的终产物)的生物合成产物。天冬氨酸形成自草酰乙酸(柠檬酸循环的中间体)。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸均通过转化天冬氨酸而形成。异亮氨酸从苏氨酸形成。组氨酸在一个复杂的9步途径中从一种活化糖——5-磷酸核糖-1-焦磷酸形成。

为细胞蛋白质合成所需的过量氨基酸不能被贮藏，并被降解以提供用于细胞主要代谢途径的中间体(综述参见Stryer，L.生物化学(Biochemistry)第三版，21章，“氨基酸降解和尿素循环”495-516页(1988))。虽然细胞可将不想要的氨基酸转化为有用的代谢中间体，从所需用于合成氨基酸的能量、前体分子和酶来说，氨基酸的产生是昂贵的。因此对于氨基酸的生物合成受到反馈抑制调节并不让人吃惊，其中特定氨基酸的存在起到减缓或完全终止其自身产生的作用(关于氨基酸生物合成途径中的反馈机制的综述参见Stryer，L.生物化学(Biochemistry)第三版，24章：”氨基酸和血红素的生物合成”575-600页(1988))。因此，任一特定氨基酸的输出受到细胞中存在的该氨基酸量的限制。

B.维生素、辅因子和营养成分的代谢和用途

维生素、辅因子和营养成分包含高等动物不能合成且必须摄取的另一分子，虽然它们易于被其它生物体如细菌合成。这些分子自身为生物活性物质或为在多种代谢途径中作为电子载体或中间体的生物活性物质的前体。除了它们的营养价值外，这些化合物作为着色剂、抗氧化剂和催化剂或其它操作辅助剂也具有重要的工业价值(这些化合物的结构、活性和工业应用的概述参见例如，Ullman工业化学百科全书(Ullman’s Encyclopediaof Industrial Chemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996)。术语”维生素”为本领域认知，包括生物体行使正常功能所需的营养素，且该生物体自身不能合成它们。维生素组和包含辅因子和营养性化合物。术语“辅因子”包括进行正常酶促活性所需的非蛋白原化合物。此类化合物可为有机的或无机的，本发明的辅因子优选有机的。术语“营养成分”包括促进植物和动物尤其是人的健康食物添加剂。此类分子的实例为维生素、抗氧化剂和某些脂类(如多不饱和脂肪酸)。

已大量描述了这些分子在可产生它们的生物体如细菌中的生物合成(Ullman工业化学百科全书(Ullman’s Encyclopedia of IndustrialChemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wiley&Sons；Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition，Lipids，Health，and Disease”Proceedings of theUNESCO/Confederation of Scientific and Technological Associations inMalaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press：Champaign，IL X，374 S)。

硫胺素(维生素B₁)由嘧啶和噻唑部分通过化学偶联产生。核黄素(维生素B₂)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素接下来用于合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。合起来称为‘维生素B₆’的化合物类(例如，吡哆素、吡哆胺、5’-磷酸吡哆醛和商业上使用的维生素B₆盐酸盐)均为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸(R)-(+)-N-(2，4-二羟基-3，3-二甲基-1-氧代丁基)-β-丙氨酸)可通过化学合成或发酵产生。泛酸生物合成的最后步骤由ATP-驱动的β-丙氨酸和泛解酸的缩合组成。负责转化为泛解酸、β-丙氨酸和泛酸缩合生物合成步骤的酶是已知的。泛酸盐的代谢活性形式为辅酶A，其生物合成由5步酶促步骤进行。泛酸盐、5’-磷酸吡哆醛、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸盐的形成，而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(维生素B₅原)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。

已详细研究了微生物中生物素从前体分子庚二酰辅酶A的生物合成，并鉴定了几种所涉及的基因。发现许多相应的蛋白质也参与Fe-簇合成，属于nifS蛋白质类。硫辛酸衍生自辛酸，并在能量代谢中作为辅酶，此时其成为丙酮酸脱氢酶复合体和á-酮戊二酸脱氢酶复合体的一部分。叶酸盐为一组这样的物质，其均为叶酸衍生物，来自于L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已详细研究了某些微生物中始于生物转化代谢中间体鸟苷-5’-三磷酸(GTP)、L-谷氨酸和对氨基苯甲酸的叶酸及其衍生物的生物合成。

类可啉(如钴胺素且尤其是维生素B₁₂)和卟啉属于一组四吡咯环体系的化学品。维生素B₁₂的生物合成太复杂，目前尚未彻底解释清楚，但许多涉及的酶和物质已知。烟酸(烟酸盐)和烟酰胺为吡啶衍生物，也称为‘烟酸’。烟酸为重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)和它们的还原形式的前体。

这些化合物的大规模产生很大程度上依赖于无细胞的化学合成，虽然这些化学品中的一些如核黄素、维生素B₆、泛酸盐和生物素也已通过微生物的大规模培养物产生。只有维生素B₁₂是仅通过发酵产生的，因为其合成的复杂性。体外方法需要耗费材料和时间，通常以高的代价耗费。

C.嘌呤、嘧啶、核苷和核苷酸的代谢和用途

嘌呤和嘧啶代谢基因及它们相应的蛋白质为治疗肿瘤和病毒感染的重要靶标。术语“嘌呤”或“嘧啶”包括构成核酸、辅酶和核苷酸的含氮碱基。术语“核苷酸”包括核酸分子的基本结构单元，其由含氮碱基、戊糖(对RNA而言，该糖为核糖，对DNA而言，该糖为D-脱氧核糖)和磷酸组成。术语“核苷”包括作为核苷酸前体的分子，但其不含核苷酸所具有的磷酸单元。通过抑制这些分子的生物合成，或抑制它们形成核酸分子的代谢，可抑制RNA和DNA合成；通过以靶向癌细胞的方式抑制这种活性，可抑制肿瘤细胞分裂和复制的能力。

此外，有不形成核酸分子但作为能量贮存体(即AMP)或作为辅酶(即FAD和NAD)的核苷酸。

一些出版物已描述了这些化学品通过影响嘌呤和/或嘧啶代谢对这些医学适应症的用途(例如Christopherson，R.I.和Lyons，S.D.(1990)”作为化学治疗剂的嘧啶和嘌呤从头生物合成的强力抑制剂”医学研究述评(Med.Res.Reviews)10：505-548)。对参与嘌呤和嘧啶代谢的酶的研究集中在研发可用作如免疫抑制剂或抗增生剂的新药上(Smith，J.L.，(1995)”核苷酸合成中的酶”结构生物学最新观点(Curr.Opin.Struct.Biol.)5：752-757；(1995)Biochem Soc.Transact.23：877-902)。但是，嘌呤和嘧啶碱基、核苷和核苷酸具有其它应用：用作一些精细化学品(例如，硫胺素、S-腺苷-甲硫氨酸、叶酸盐或核黄素)生物合成的中间体，作为细胞的能量载体(例如，ATP或GTP)，和本身作为化学品，通常作为增味剂(例如，IMP或GMP)或用于一些医药用途(参见例如，Kuninaka，A.(1996)生物技术中的核苷酸和相关化合物(Nucleotides and Related Compounds in Biotechnology)第6卷，Rehm等人编辑.VCH：Weinheim，561-612页)。同样，参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作靶，研发用于作物保护的抗这些酶的化学品包括杀真菌剂、除草剂和杀虫剂。

以阐释了这些化合物在细菌中的代谢(综述参见例如，核酸研究和分子生物学进展(Progress in Nucleic Acid Research and Molecular Biology)，第42卷，Academic Press中的Zalkin，H.和Dixon，J.E.(1992)”嘌呤核苷酸的从头生物合成”，259-287页；和生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：纽约一书中的Michal，G.(1999)”核苷酸和核苷”，第8章)。嘌呤代谢已是深入研究的主题，其对细胞的正常功能是必需的。高等动物中受损的嘌呤代谢可引起严重疾病，如痛风。嘌呤核苷酸从核糖-5-磷酸开始，经过一系列步骤，通过中间体化合物次黄苷-5’-磷酸(IMP)，产生鸟苷-5’-单磷酸(GMP)或腺苷-5’-单磷酸(AMP)，由此易于形成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存体，这样它们的降解可提供细胞内用于许多不同生物化学过程的能量。嘧啶的生物合成由核糖-5-磷酸形成尿苷-5’-单磷酸(UMP)而进行。UMP接下来转化为胞苷-5’三磷酸(CTP)。所有这些核苷酸的脱氧形式通过一步还原步骤从核苷酸的二磷酸核糖形式产生为核苷酸的二磷酸脱氧核糖形式。通过磷酸化后，这些分子可参与DNA合成。

D.海藻糖的代谢和用途

海藻糖由两个葡萄糖分子通过α，α-1，1连接组成。它通常作为甜味剂用于食品工业，为干的或冷冻食品和饮料中的添加剂。但它也用于药学、化妆品和生物技术工业(参见例如，Nishimoto等人(1998)美国专利号5,759,610；Singer，M.A.和Lindquist，S.生物技术趋势(Trends Biotech.)16(1998)：460-467；Paiva，C.L.A.和Panek，A.D.Biotech.Ann.Rev.2(1996)：293-314；和Shiosaka，M.J.Japan 172：(1997)97-102)。海藻糖通过酶从许多微生物产生，并天然释放入环境介质中，从中可用本领域公知的方法收集海藻糖。

II.代谢调节机制

所有活细胞都由复杂的分解代谢和合成代谢能力，其中许多代谢途径相互关联。为了维持该异常复杂的代谢网络的各部分之间的平衡，细胞采用了精密调节的调节系统。通过独立地或同时地调节酶的合成和酶活性，细胞能够调节完全不同的代谢途径的活性以便满足细胞不断变化的需要。

可以在转录水平或翻译水平或者这两种水平上诱导或者抑制酶的合成(综述见Lewin，B.(1990)Genes IV，第3部分：“通过转录调控原核细胞基因(Controlling prokaryotic genes by transcription)”，牛津大学出版社，牛津，213-301页，及其参考文献，和Michal，G.(1999)，生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistryand Molecular Biology)，John Wiley & Sons)。所有这些已知的调节过程都被应答多种外部影响(例如温度、营养供给或光)的其他基因介导。参与这种类型调节的蛋白因子的例子包括转录因子。它们是结合DNA从而导致基因表达或者增加(正调节，如大肠杆菌ara操纵子)或者降低(负调节，如大肠杆菌lac操纵子)的蛋白质。这些调节表达的转录因子本身可受到调节。例如，它们的活性可受到结合DNA结合蛋白的低分子量化合物的调节，从而所述蛋白对DNA上合适的结合位点的结合被刺激(如ara操纵子的阿拉伯糖)或抑制(如lac操纵子的乳糖)(见，例如，Helmann，J.D.和Chamberlin，M.J.(1988)，“细菌σ因子的结构和功能(Structure andfunction of bacterial sigma factors)”Ann.Rev.Biochem.57：839-872；Adhya，S.(1995)“今日lac和gal操纵子(The lac and gal operons today)”以及Boos，W.等人，“麦芽糖体系(The maltose system)”，这两篇文章在大肠杆菌中基因表达的调节(Regulation of Gene Expression in Escherichiacoli)一书中，由Lin，E.C.C.和Lynch，A.S.编辑，Chapman & Hall：NewYork，181-200页和201-229页；和Moran，C.P.(1993)“RNA聚合酶和转录因子(RNA polymerase and transcription factors)”，在：枯草芽孢杆菌和其他革兰氏阳性菌(Bacillus subtilis and other Gram-positive bacteria)一书中，由Sonenshein，A.L.编辑ASM：Washington D.C.653-667页)。

蛋白质的合成不仅在转录水平而且在翻译水平受到调节。可以通过许多机制进行调节，包括对核糖体结合一种或多种mRNA能力的修饰、核糖体与mRNA的结合、保持或去除mRNA二级结构、对特定基因使用常见或不常见密码子、一种或多种tRNA的丰度和特定调节机制如弱化作用进行调节(见Vellanoweth，R.I.(1993)枯草芽孢杆菌和其他革兰氏阳性菌中的翻译及其调节(Translation and its regulation in Bacillus subtilis andother Gram-positive bacteria)，Sonenshein，A.L.等人编辑ASM：Washington，D.C.，699-711页及其参考文献)。

可以对各种代谢途径中的单个蛋白(相继调节)或同时对多种蛋白(协同调节)进行转录和翻译调节。表达受到协同方式调节的基因通常位于基因组中操纵子或调节子接近处。这种基因转录和基因翻译的上调或下调受到多种因素如底物(在一种或多种代谢途径中使用的前体和中间体)、分解代谢物(与从复杂有机分子如糖的降解的能量产生相关的生物化学途径产生的分子)和终产物(在代谢途径末得到的分子)的细胞或细胞外的量控制。编码特定代谢途径的活性所需的酶的基因表达被所述代谢途径的大量底物分子所诱导。相应地，该基因表达被胞内大量该途径的终产物所抑制(Snyder，L.和Champness，W.(1977)，细菌的分子生物学(TheMolecular Biology of Bacteria)ASM：Washington)。基因表达同样可被其他外部或内部因素如环境条件(例如热、氧化性应急或饥饿)所调节。这种全局环境变化导致专门的调节基因表达的变化，这些调节基因通过结合DNA而直接或间接(通过其他基因或蛋白)引发基因表达，从而诱导或抑制转录(见，例如，Lin，E.C.C.和Lynch，A.S.编辑(1995)，大肠杆菌中基因表达的调节(Regulation of Gene Expression in Escherichia coli)，Chapman & Hall：New York)。

调节细胞代谢的另一个机制发生在蛋白质水平。该调节通过其他酶的活性或通过结合阻止或赋予该蛋白正常功能的低分子量组分进行。通过结合低分子量化合物进行蛋白调节的例子包括GTP或NAD的结合。低分子量化学物质的结合通常是可逆的，例如GTP结合蛋白。这些蛋白存在两种状态(结合GTP或GDP)，一种状态是蛋白质的活性形式，另一中是无活性形式。

蛋白活性被其他酶的作用调节，其中其他酶通常通过对蛋白的共价修饰(即氨基酸残基如组氨酸或天冬氨酸的磷酸化或甲基化)调节。这种共价修饰通常是可逆的并且受到具有相反活性的酶影响。一个例子是激酶和磷酸酶在蛋白质磷酸化中的相反活性：蛋白激酶在靶蛋白上(例如丝氨酸或苏氨酸)磷酸化特定残基，而蛋白磷酸酶从所述蛋白除去磷酸基团。调节其他蛋白活性的酶本身通常受到外界刺激的调节。这些刺激被作为传感器的蛋白所介导。被这些传感器蛋白介导外界信号的一个熟知的机制是二聚体化，但也知道其他机制(见，例如Msadek，T.等(1993)“双组分调节体系(Two-component Regulatory Systems)”在：枯草芽孢杆菌和其他革兰氏阳性菌(Bacillus subtilis and Other Gram-Positive Bacteria)一书中，Sonenshein，A.L.等人编辑，ASM：Washington，729-745页及其参考文献)。

详细了解调控微生物中细胞代谢的调节网络对通过发酵高产量地生产化学品是关键的。可以去除或降低下调代谢途径的调控系统以便提高目标化学品的合成，相应地，可以组成性地活化或优化上调目标产物的代谢途径的调控系统的活性(如Hirose，Y.和Okada，H.(1979)“氨基酸的微生物生产(Microbial Production of Amino Acids)”，在：Peppler，H.J.和Perlman，D.(编辑)微生物技术(Microbial Technology)第二版，第1卷，第7章，Academic Press，New York)。

III.本发明的组成要件和方法

本发明至少部分是基于本文中称作MP核酸和MP蛋白分子的新分子的检测，这些分子通过转录、翻译或翻译后的措施调节谷氨酸棒状杆菌中的一种或多种代谢途径。在一个实施方案中，MP分子转录地、翻译地或翻译后地调节谷氨酸棒状杆菌中的代谢途径。在一个优选的实施方案中，调节谷氨酸棒状杆菌中的一种或多种代谢途径的本发明MP分子的活性影响所述生物体的目标精细化学品的生产率。在一个尤其优选的实施方案中，本发明的MP分子具有调节的活性，使得被本发明MP蛋白调节的谷氨酸棒状杆菌代谢途径的效率或产量受到调节，并且这直接或间接地调节谷氨酸棒状杆菌的目标精细化学品的产量、生产率和/或生产效率。

术语“MP蛋白”或“MP多肽”包括转录中、翻译中或者翻译后调节谷氨酸棒状杆菌中代谢途径的蛋白。MP蛋白的例子包括表1和附录A中所列出的MP基因编码的那些蛋白。术语“MP基因”和“MP核酸序列”包括编码MP蛋白的核苷酸序列，其含有一个编码区和相应的非翻译5’和3’序列区。MP基因的例子在表1中列出。术语“生产率”为本领域已知并且包括发酵产物的浓度(例如在预定的时间段和预定的发酵体积产生的目标精细化学品(例如kg产物/h/l))的浓度。术语“生产效率”包括得到特定产物量所需时间(例如，细胞达到精细化学品的特定生产率所需时间)。术语“产量”或“产物/碳产量”为本领域已知并且包括将碳源转化成产物(即精细化学品)的效率。这例如通常表达为kg产物/kg碳源。增加化合物的产量或生产率为在预定期间内及特定的培养体积中增加了所得分子的量或该化合物适宜得到的分子的量。术语“生物合成”和“生物合成途径”为本领域已知并且包括细胞在例如多步过程或高度调节的过程中从中间物合成化合物，优选有机化合物。术语“降解”和“降解途径”为本领域已知并且包括细胞在例如多步过程或高度调节的过程中将化合物，优选有机化合物裂解成降解产物(用更通俗的术语：更小或更简单的分子)。术语“代谢”为本领域已知并且包括生物体中发生的全部生物化学反应。特定化合物的代谢(例如氨基酸如甘氨酸的代谢)因此包括细胞中该化合物的所有生物合成、修饰和降解途径。术语“调节”为本领域已知并且包括调控另一蛋白质活性的蛋白质的活性。术语“转录调节”为本领域已知并且包括抑制或活化编码靶蛋白的DNA转化成mRNA的蛋白质的活性。术语“翻译调节”为本领域已知并且包括抑制或活化编码靶蛋白的mRNA转化成蛋白分子的蛋白质的活性。术语“翻译后调节”为本领域已知并且包括通过共价修饰靶蛋白(例如通过甲基化、糖基化或磷酸化)抑制或提高靶蛋白活性的蛋白质的活性。

在另一个实施方案中，微生物如谷氨酸棒状杆菌中本发明的MP分子能够调节目标分子例如精细化学品的生产率。借助于基因重组技术，可操作一种或多种本发明的代谢途径调节蛋白使得它们的功能被调节。可以例如提高生物合成酶的效率或者破坏其变构调控区以便阻止该化合物产生的反馈抑制。所以，可以通过替换、缺失或添加来删除或修饰降解酶使得其对目标化合物的降解活性降低而不损害细胞的存活。在任何情况下，可以增加任何所述目标精细化学品的总产量或生产率。

还可能的是本发明的蛋白和核苷酸分子的这些修饰能够间接提高精细化学品的生产率。细胞中代谢途径的调节机制不可避免地相关联，一种代谢途径的激活能够伴随着抑制或激活另一种代谢途径。调节本发明的一种或多种蛋白的活性能影响其他精细化学品生物合成或降解途径活性的生产率或效率。降低一种MP蛋白抑制编码氨基酸生物合成中特定蛋白的基因转录使得可能同时抑制其他氨基酸生物合成途径，因为这些代谢途径相互关联。通过调节本发明MP蛋白可能使细胞生长和细胞分裂与细胞胞外环境以一定程度解偶联；当用于生长和细胞分裂的胞外条件不是最佳时某种MP蛋白通常抑制核苷酸的生物合成，通过影响该MP蛋白使其失去抑制核苷酸合成的功能，可能使得即使胞外条件较差时仍能生长。这对于大规模发酵培养尤其重要，大规模发酵的温度、营养供给或通气通常不是最佳的，但是当所述因子的细胞调节系统被去除后仍然能够促进生长和细胞分裂。

制备本发明核酸序列的一个适宜的起点是谷氨酸棒状杆菌菌株的基因组，可以从美国典型培养物保藏中心得到该菌株(名称是ATCC 13032)。

用常规方法，通过表1所示的修饰可以从这些核酸序列制备本发明的核酸序列。

本发明的MP蛋白或其生物学活性部分或片段能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径或可以具有表1列出的一种或多种活性。

下面的分段详细描述本发明的各方面：

A.分离的核酸分子

本发明的一个方面涉及编码MP多肽或其生物活性部分的分离核酸分子及核酸分子片段，其中所述核酸分子片段足以用作杂交探针或引物，用来鉴定或扩增编码MP的核酸(例如MP DNA)。如此处所用，术语“核酸分子”是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)和用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’和5’端的非翻译区：基因编码区5’端上游的至少约100个核苷酸序列和基因编码区3’端下游的至少约20个核苷酸序列。核酸分子可为单链或双链，但优选地为双链DNA。“分离的”核酸分子为从其它核酸分子分离的核酸分子，其存在于核酸的天然源中。优选地，“分离的”核酸不具有该核酸所来自的生物体基因组DNA中天然存在于该核酸侧翼的序列(即位于该核酸5’和3’端的序列)。例如，在不同实施方案中，该分离的MP核酸分子可包含少于约5kb，4kb，3kb，2kb，1kb，0.5kb或0.1kb的该核酸所来自的细胞(如谷氨酸棒状杆菌细胞)基因组DNA中天然存在于该核酸分子侧翼的核苷酸序列。而且，“分离的”核酸分子如cDNA分子当通过重组技术产生时可基本上不含有其它细胞物质或培养基，当通过化学合成产生时，可基本上不含有化学前体或其它化学品。

本发明的核酸分子，如具有的核苷酸序列为附录A的核酸分子或其部分可用标准的分子生物学技术和本文提供的序列信息制备。例如，使用附录A全部或部分序列作为杂交探针和标准的杂交技术(例如，如在Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，冷泉港实验室，冷泉港实验室出版社，冷泉港，纽约，1989中所述)，可自谷氨酸棒状杆菌文库分离谷氨酸棒状杆菌MP DNA。而且，包含附录A的全部序列或其部分的核酸分子可使用根据该序列设计的寡核苷酸引物，应用聚合酶链反应分离(例如，包含附录A的全部或部分的核酸分子可使用根据该同样序列设计的寡核苷酸引物，应用聚合酶链反应分离)。例如，可从正常的内皮细胞分离mRNA(例如，通过Chirgwin等人(1979)生物化学(Biochemistry)18：5294-5299的硫氰酸胍提取法)以及用反转录酶(例如，莫洛尼鼠类白血病毒反转录酶，可从Gibco/BRL，Bethesda，MD获得；或AMV反转录酶，可从Seikagaku America，Inc.，St.Petersburg，FL获得)可制备cDNA。用于聚合酶链反应扩增的合成寡核苷酸引物可根据附录A所示的核苷酸序列之一设计。本发明的核酸可用cDNA或另外用基因组DNA作为模板、使用合适的寡核苷酸引物按照标准的PCR扩增技术来扩增。这样扩增的核酸可克隆入合适的载体并通过DNA序列分析鉴定。对应于MP核苷酸序列的寡核苷酸可通过标准的合成技术制备，例如用DNA自动合成仪制备。

在一个优选的实施方案中，本发明的分离核酸分子包含附录A中所示的任一核苷酸序列。

在另一个优选的实施方案中，本发明的分离的核酸分子含有与附录A中所示核酸序列之一互补的核酸分子，所述核酸分子与附录A中所示核酸序列之一足够互补使其与附录A中所示序列之一杂交而得到稳定双链体。

在一个实施方案中，本发明的核酸分子编码这样的蛋白质或其部分，其包括的氨基酸序列充分同源于附录B的一个氨基酸序列，以至于该蛋白质或其部分仍然能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径。如此处所用，术语“充分同源”是指蛋白质或其部分所具有的氨基酸序列包括最小数的与本发明氨基酸序列相同或等同的氨基酸残基(例如，氨基酸残基具有与附录B中序列之一的氨基酸残基相似的侧链)，以至于该蛋白质或其部分能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径。此类代谢途径中的蛋白质成员如此处所述，可调节一种或多种精细化学品的生物合成或降解。此类活性的实例也在此进行了描述。因此，“MP蛋白的功能”涉及一种或多种精细化学品代谢途径的总体调节。MP蛋白活性的实例在表1中给出。

本发明MP核酸分子编码的蛋白部分优选地是任何MP蛋白的具生物学活性的部分。文中所用术语“MP蛋白的生物学活性部分”包含MP蛋白的一部分，例如结构域或基元，其能够在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径或者具有表1中所示活性。为了确定MP蛋白或者其生物学活性部分能否在转录中、翻译中或翻译后调节谷氨酸棒状杆菌的代谢途径，可以进行酶活性分析。这些分析方法在实施例部分的实施例8中详细描述，技术人员熟知这些方法。

除了群体中存在的MP序列的天然变体外，技术人员还理解可通过突变向附录A的核苷酸序列中导入变化，由此导致所编码的MP蛋白的氨基酸序列改变，但MP蛋白的功能不变。例如，可在附录A的核苷酸序列中进行在“非必需”氨基酸残基处导致氨基酸替换的核苷酸替换。可以修饰任一MP蛋白(附录B)的野生型序列中的“非必需”氨基酸残基而不改变所述MP蛋白的活性，但“必需”氨基酸残基为MP蛋白活性所需。然而，其它氨基酸残基(例如，在具有MP活性的结构域中不保守或仅为半保守的那些氨基酸残基)对活性可为非必需的，并因此可被改变，而此类氨基酸残基的改变不改变MP活性。

编码同源于附录B的蛋白质序列的MP蛋白的分离核酸分子可通过将一个或多个核苷酸替换、添加或删除导入附录A的核苷酸序列而产生，这样将一个或多个氨基酸的替换、添加或删除导入所编码的蛋白质。可通过标准技术将突变导入附录A的核苷酸序列之一，如定点诱变和PCR-介导的诱变。优选地，在一处或多处预测的非必需氨基酸残基处进行保守氨基酸替换。“保守氨基酸替换”为将氨基酸残基用具有相似侧链的氨基酸残基替换。具有相似侧链的氨基酸残基类已在本领域定义过。这些分类包括具有碱性侧链的氨基酸(例如，赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如，天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如，甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如，丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如，苏氨酸、缬氨酸、异亮氨酸)和具有芳香侧链的氨基酸(例如，酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此，在MP蛋白中预测的非必需氨基酸残基优选地用来自同一侧链类的另一氨基酸残基替换。在另一实施方案中，可如通过饱和诱变沿着全部或部分MP编码序列随机导入突变，并对所得突变体筛选文中所述的MP活性，以鉴定保留MP活性的突变体。对任一附录A的序列诱变后，可重组表达编码蛋白，并用例如文中所述的试验(参见实施例部分的实施例8)确定所述蛋白质的活性。

B.重组表达载体和宿主细胞

本发明的另一方面涉及包含编码MP蛋白(或其部分)的核酸或包含基因组合且其中至少一种基因编码MP蛋白的载体，优选表达载体。如此处所用，术语“载体”是指这样的核酸分子，其可转运与其连接的另一核酸。一种类型的载体为“质粒”，是指其中可连接额外DNA片段的环状双链DNA环。另一种类型的载体为病毒载体，其中可将额外DNA片段连接入病毒基因组。某些载体可在它们所导入的宿主细胞中自主复制(例如，具有细菌复制起点的细菌载体和游离型哺乳动物载体)。其它载体(例如，非游离型哺乳动物载体)在导入宿主细胞后整合入宿主细胞的基因组，并因此随着宿主基因组而复制。而且，某些载体可指导与它们操作性连接的基因的表达。此类载体此处称为“表达载体”。一般，用于重组DNA技术中的表达载体通常为质粒形式。在本说明书中，“质粒”和“载体”可互换使用，因为质粒是最经常使用的载体形式。但是，本发明旨在包括其它形式的表达载体，如病毒载体(例如，复制缺陷型反转录病毒、腺病毒和腺相关病毒)，它们起到类似的作用。

本发明的重组表达载体以适于在宿主细胞中表达核酸的形式包含本发明的核酸，这就是说重组表达载体包括一个或多个调节序列，其选择是基于用作表达的宿主细胞进行的，它们可操作地与待表达的核酸序列连接。在重组表达载体中，术语“可操作地连接”是指目的核苷酸序列与调节序列以允许该核苷酸序列表达(例如，在体外转录/翻译系统中，或当载体导入宿主细胞时在宿主细胞中表达)的方式连接。术语“调节序列”是指包括启动子、增强子和其它表达调控元件(例如，多聚腺苷酸化信号)。此类调节序列如在Goeddel；基因表达技术：酶学方法(GeneExpressionTechnology：Methods in Enzymology)185，Academic Press，SanDiego，CA(1990)中描述。调节序列包括在许多类型宿主细胞中控制核苷酸序列组成型表达的调节序列和仅在某些宿主细胞中控制核苷酸序列直接表达的调节序列。本领域普通技术人员了解到表达载体的设计可取决于如对欲转化宿主细胞的选择、所需的蛋白质表达程度等因素。本发明的表达载体可导入宿主细胞，以产生由此处所述核酸所编码的蛋白质或肽，包括融合蛋白或融合肽(例如，MP蛋白、MP蛋白的突变形式、融合蛋白等)。

本发明的重组表达载体可设计用于在原核或真核细胞中表达MP蛋白。例如，MP基因可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母细胞和其它真菌细胞(参见Romanos，M.A.等人(1992)“在酵母中外源基因的表达：综述”(“Foreign gene Expression in yeast：areview”)，酵母(Yeast)8：423-488；J.W.Bennet & L.L.Lasure编辑的更多的用于真菌的基因操作(More Gene Manipulations in Fungi)，AcademicPress：San Diego一书中的van den Hondel，C.A.M.J.J.等人(1991)“丝状真菌中的异源基因表达”(“Heterologous gene Expression in filamentousfungi”)，396-428页；以及Peberdy，J.F.等人编辑的真菌的应用分子遗传学(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfer systems and vectordevelopment for filamentous fungi)，1-28页)、藻类和单细胞植物细胞(参见Schmidt，R.和Willmitzer，L.(1988)High efficiency Agrobacteriumtumefaciens-mediated transformation of Arabidopsis thaliana leaf andcotyledon explants”植物细胞报道(Plant Cell Rep.)：583-586)、或哺乳动物细胞中表达。合适的宿主细胞在Goeddel，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，AcademicPress，San Diego，CA(1990)中进一步进行了讨论。另外，可体外转录并翻译重组表达载体，如使用T7启动子调节序列和T7聚合酶。

在原核生物中蛋白质的表达主要用含有组成型或诱导型启动子的载体进行，控制融合或非融合蛋白的表达。融合载体将一些氨基酸添加至其中编码的蛋白质上，通常添加至重组蛋白的氨基末端。此类融合载体一般具有三个任务：1)用于增强重组蛋白的表达；2)用于增加重组蛋白的溶解性；和3)在亲和层析中作为配体而利于重组蛋白的纯化。通常，于融合部分和重组蛋白的连接处导入蛋白酶剪切位点到融合表达载体中，这使得在该融合蛋白纯化后，可将重组蛋白从融合单元分离。这些酶和它们的对应识别序列包括Xa因子、凝血酶和肠激酶。

通常的融合表达载体包括pGEX(Pharmacia Biotech Inc；Smith，D.B.和Johnson，K.S.(1988)基因(Gene)67：31-40)、pMAL(New EnglandBiolabs，Beverly，MA)和pRIT5(Pharmacia，Piscataway，NJ)，它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白A结合至重组靶蛋白。在一个实施方案中，将MP蛋白的编码序列克隆入pGEX表达载体，以产生编码融合蛋白的载体，其从N-末端至C-末端包含GST-凝血酶切割位点-X蛋白。该融合蛋白使用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。通过将该融合蛋白用凝血酶切割可得到不与GST融合的重组MP蛋白。

合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等人(1988)基因(Gene)69：301-315)和pET 11d(Studier等人，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)60-89)。pTrc载体中目标基因的表达依赖于宿主RNA聚合酶从杂合的trp-lac融合启动子的转录。pET 11d载体中目标基因的表达依赖于共表达的病毒RNA聚合酶(T7 gn1)介导的从T7 gn10-lac融合启动子的转录。该病毒聚合酶由宿主菌株BL21(DE3)或HMS174(DE3)中居住的、具有位于lacUV 5启动子转录调控下的T7 gn1基因的λ前噬菌体提供。

最大化重组蛋白表达的一个策略是将该蛋白质在具有对所述重组蛋白进行蛋白酶切割的能力缺损的宿主细菌中表达(Gottesman，S.，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)119-128)。另一策略是改变欲插入表达载体的核酸的核酸序列，以使用于每一氨基酸的单个密码子为选择用于表达的细菌如谷氨酸棒状杆菌所优选使用的密码子(Wada等人(1992)核酸研究(Nucleic Acids Res.)20：2111-2118)。本发明核酸序列的此改变可应用标准的DNA合成技术实施。

在另一实施方案中，MP蛋白表达载体为酵母表达载体。用于在酿酒酵母中表达的载体包括pYepSec1(Baldari，等人(1987)Embo J.6：229-234)，pMFa(Kurjan和Herskowitz，(1982)细胞(Cell)30：933-943)，pJRY88(Schultz等人(1987)基因(Gene)54：113-123)，和pYES2 (InvitrogenCorporation，San Diego，CA)。适用于其它真菌如丝状真菌的载体和用于构建载体的方法包括在：J.F.Peberdy等人编辑的真菌的应用分子遗传学(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfer systems and vectordevelopment for filamentous fungi)，1-28页中详述的那些。

另外，本发明的MP蛋白可使用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf 9细胞)中表达蛋白质的杆状病毒载体包括pAc系列(Smith等人(1983)Mol.Cell Biol.3：2156-2165)和pVL系列(Lucklow和Summers(1989)病毒学(Virology)170：31-39)。

在另一实施方案中，本发明的MP蛋白可在单细胞植物细胞(如藻类)或在来自高等植物(如种子植物，诸如作物)的植物细胞中表达。植物表达载体的实例包括在：Becker，D.，Kemper，E.，Schell，J.和Masterson，R.(1992)“具有靠近左边界的选择性标记的新植物双元载体(”New plantbinary vectors with selectable markers located proximal to the leftborder”)，植物分子生物学(Plant Mol.Biol.)20：1195-1197；和Bevan，M.W.(1984)“用于植物转化的双元农杆菌载体”(”Binary Agrobacteriumvectors for plant transformation”)，核酸研究(Nucl.Acid.Res.)12：8711-8721中详述的那些。

在另一实施方案中，本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed，B.(1987)自然(Nature)329：840)和pMT2PC(Kaufman等人(1987)EMBO J.6：187-195)。当用于哺乳动物细胞时，表达载体的调控功能通常由病毒调控元件提供。例如，常用的启动子来自多瘤、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它合适表达系统参见Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：ALaboratory Manual)，第二版，Cold Spring Harbor Laboratory，ColdSpring Harbor Laboratory Press，Cold Spring Harbor，NY，1989中的第16和17章。

在另一实施方案中，重组哺乳动物表达载体可优选地在特定细胞类型中指导核酸的表达(例如，使用组织-特异性调控元件来表达核酸)。组织-特异性调控元件为本领域公知。合适的组织-特异性启动子的非限制性实例包括白蛋白启动子(肝-特异性；Pinkert等人(1987)基因进展(Genes Dev.)1：268-277)、淋巴-特异性启动子(Calame and Eaton(1988)Adv.Immunol.43：235-275)，特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8：729-733)和免疫球蛋白启动子(Banerji等人(1983)细胞(Cell)33：729-740；Queen和Baltimore(1983)细胞(Cell)33：741-748)、神经元-特异性启动子(例如，神经丝启动子；Byrne和Ruddle(1989)PNAS86：5473-5477)、胰-特异性启动子(Edlund等人(1985)科学(Science)230：912-916)、以及乳腺-特异性启动子(例如，奶乳清启动子；美国专利号4,873,316和欧洲申请公开号264,166)。也同样包括调节发育的启动子，如鼠hox启动子(Kessel和Gruss(1990)科学(Science)249：374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)基因进展(Genes Dev.)3：537-546)。

本发明还提供了含本发明的DNA分子的重组表达载体，其中该DNA分子以反义方向克隆在表达载体中。也就是，DNA分子功能性地连接到调控序列上，该连接方式使得可以表达产生与MP mRNA反义的RNA分子(通过转录该DNA分子)。可以对功能性地连接反义方向克隆的核酸的调控序列进行选择以控制反义RNA分子在多种细胞类型中持续表达，例如，可选择病毒启动子和/或增强子或调控序列以控制反义RNA实现组成型组织特异性或细胞类型特异性表达。反义表达载体的形式可为重组质粒、噬菌粒或减毒病毒，其中在高效调控区域的控制下产生反义核酸，其活性由载体所导入细胞的类型决定。反义基因的基因表达调控的讨论见Weintraub，H.等人，反义RNA作为遗传分析的分子工具，Reviews-Trendsin Genetics，卷1(1)1986。

本发明的另一方面涉及已导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此处可互换使用。当然，该术语不仅指特定的受试细胞，也指该细胞的后代或潜在后代。由于突变或环境因素在传代过程中可发生某些改变，这样，后代实际上可能并非与亲代细胞相同，但其仍包括在此处所用术语的范围之内。

宿主细胞可为任意的原核或真核细胞。例如，MP蛋白可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢(CHO)细胞或COS细胞)中表达。其他合适的宿主细胞为技术人员公知。与谷氨酸棒状杆菌有关的、可常规用作本发明核酸和蛋白质分子的宿主细胞的微生物如表3所示。

载体DNA可通过常规转化或转染技术导入原核或真核细胞中。如此处所用，术语“转化”和“转染”意指本领域内公知的各种将外源核酸(例如DNA)导入宿主细胞的技术，包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖-介导的转染、脂转染、电穿孔。转化或转染宿主细胞的合适方法可在Sambrook等人(分子克隆：实验室手册(Molecular Cloning：A LaboratoryManual).第二版，Cold Spring Harbor Laboratory，Cold Spring HarborLaboratory Press，Cold Spring Harbor，NY，1989)和其它的实验室手册中找到。

对于哺乳动物细胞的稳定转染，已知根据所用的表达载体和所用的转染技术，仅一小部分的细胞可将外源DNA整合到基因组中。一般将编码选择标记的基因(如抗生素抗性基因)与目的基因一同导入宿主细胞以鉴定和筛选整合体。优选的选择标记包括那些可赋予药物抗性的基因，所述药物如G418、潮霉素和氨甲蝶呤。编码选择标记的核酸可与编码MP蛋白质的基因位于同一载体上被导入宿主细胞或可以位于不同的载体上导入。经导入核酸稳定转染的细胞可通过药物筛选进行鉴定(例如，掺入了选择标志基因的细胞将存活，而其他细胞死亡)。

通过制备出含至少部分MP基因的载体，所述部分MP基因中已经导入缺失、添加或替代因而可以改变MP基因(如功能性破坏)来生成同源重组微生物。优选地该MP基因为谷氨酸棒状杆菌MP基因，但也可为来自相关细菌或甚至来自哺乳动物、酵母或昆虫的同系物。在一个优选的实施方案中，载体设计为在同源重组后可功能性破坏内源MP基因(即，不再编码功能蛋白质；也称为“敲除”载体)。另外，载体可设计为在同源重组后内源MP基因发生突变或改变但仍编码功能蛋白质(例如，可改变上游调控区域由此改变内源MP蛋白的表达)。在此同源重组载体中，改变的MP基因部分在其5′和3′端侧翼为MP基因的其它核酸，从而允许在载体携带的外源MP基因和微生物中的内源MP基因之间发生同源重组。此其它的两翼MP核酸的长度应足以使与内源基因的重组成功进行。通常，载体内包括几千碱基的侧翼DNA(5′及3′端)(参见例如，Thomas，K.R.，和Capecchi，M.R.(1987)细胞(Cell)51：503中对同源重组载体的描述)。使用本领域公知的方法，将该载体导入微生物(例如，通过电穿孔)和细胞，筛选其中导入的MP基因已与内源MP基因同源重组的微生物和细胞。

在另一实施方案中，产生的重组微生物可含有可调控导入基因表达的所选系统。例如，将在载体中包含的MP基因插入乳糖操纵子的控制下，使得MP基因仅在存在IPTG时表达。此类调控系统为本领域熟知。

本发明的宿主细胞如培养的原核或真核宿主细胞可用于产生(即表达)MP蛋白。因此，本发明还提供了使用本发明的宿主细胞来产生MP蛋白的方法。在一个实施方案中，该方法包括在合适的培养基中培养本发明的宿主细胞(其中已导入了编码MP蛋白的重组表达载体，或其中基因组已导入了编码野生型或改变的MP蛋白的基因)，直至产生MP蛋白。在另一实施方案中，该方法还包括从培养基或宿主细胞分离MP蛋白。

C.本发明的用途和方法

文中所述的核酸分子、蛋白质、蛋白质同系物、融合蛋白、引物、载体和宿主细胞可用于一种或多种下述方法中：鉴定谷氨酸棒状杆菌及相关生物体；绘制与谷氨酸棒状杆菌相关的生物体的基因组图谱；鉴定并定位目标谷氨酸棒状杆菌序列；进化研究；确定为MP蛋白所需的功能区域；调节MP蛋白活性；调节MP途径的活性；以及调节目标化合物如精细化学品的细胞生产率。本发明的MP核酸分子具有多种用途。首先，它们可用于鉴定生物体为谷氨酸棒状杆菌或其近亲。它们也可用于在混合的微生物群体中鉴定谷氨酸棒状杆菌或其相关菌的存在。本发明提供了许多谷氨酸棒状杆菌基因的核酸序列。通过将微生物单一群体或混合群体的培养物提取的基因组DNA在严格条件下与包含对谷氨酸棒状杆菌独特的谷氨酸棒状杆菌基因区域的探针杂交，可确定该生物体是否存在。虽然谷氨酸棒状杆菌自身为非致病的，但它与致病种如白喉棒状杆菌有亲缘关系。这种生物体的检测具有显著的临床重要性。

本发明的核酸和蛋白质分子也可用作基因组特异性区域的标记。它不仅可用于基因组图谱，也可用于谷氨酸棒状杆菌蛋白质的功能研究。例如，可切割谷氨酸棒状杆菌基因组，并将片段与DNA-结合蛋白孵育来确定与特定的谷氨酸棒状杆菌DNA-结合蛋白结合的基因组区域。另外，那些结合蛋白的片段可用本发明的核酸分子探测，优选地用具有易于检测的标记物的核酸分子探测；此核酸分子与基因组片段的结合可将片段定位于谷氨酸棒状杆菌的基因组图谱中，并且当用不同的酶进行多次时，利于快速确定蛋白质结合的核酸序列。而且，本发明的核酸分子可充分同源于亲缘种的序列，这样这些核酸分子可用作在亲缘菌如乳糖发酵短杆菌中构建基因组图谱的标记。

本发明的MP核酸分子也用于进化研究和蛋白质结构研究。大量的原核和真核细胞应用本发明分子参与的代谢过程；通过将本发明核酸分子的序列与那些来自其它生物体的编码类似酶的序列比较，可评估所述生物体的进化关系。所以，此比较使得可评估哪些区域保守和哪些区域不保守，这有利于确定那些对酶功能所必需的蛋白质区域。这种确定对蛋白质工程研究有价值，并可指出该蛋白质可耐受何种诱变且不丢失其功能。

本发明MP核酸分子的操作可导致产生与野生型MP蛋白功能不同的MP蛋白。这些蛋白质可在功效或活性上提高，在细胞内可以较通常多的量存在，或可在功效或活性上降低。

这些活性的改变可以是使得谷氨酸棒状杆菌的一种或多种精细化学品的产量、生产率和/或生产效率提高的改变。通过优化活化编码目标精细化学品的生物合成蛋白的基因的转录或翻译或者通过影响或删除抑制这种基因转录或翻译的MP蛋白的活性，可能增加该生物合成途径的活性或活性率，归功于例如一种限制酶的量增加。相应地，可以通过改变MP蛋白的活性，使得其组成性地转录后失活参与目标精细化学品的降解途径的蛋白或者通过改变MP蛋白的活性，使得其组成性地抑制这种基因的转录或翻译以增加细胞的所述精细化学品的产量和/或生产率，这归功于该化合物的降解减少。

调节一种或多种MP蛋白的活性使得可能间接刺激细胞的一种或多种精细化学品的生产或者提高生产率，因为各种代谢途径相关联。可例如通过活化赖氨酸生物合成中一种或多种酶的表达来增加产量、生产率和/或生产效率以同时增加其他化合物的表达，例如其他氨基酸，当需要更大量的赖氨酸时细胞通常需要更大量的这些化合物。还可以改变整个细胞中的代谢调节，使得发酵培养物(其中营养和氧气的供给可能较差并且该环境中还可能大量存在毒性废物)的环境条件中的细胞的生长和复制改善。从而可例如改善培养物中细胞的生长和增殖，即使生长条件不是最佳，通过诱变某种MP蛋白(其针对胞外培养基中的高水平废产物抑制细胞膜生长所需的分子的合成以便阻碍非最佳生长条件中的细胞生长和细胞分裂)使得所述蛋白不再能够抑制所述合成。这种生长增加或这种存活增加将同样增加发酵培养物中目标精细化学品的产量和/或生产率，因为在给培养物中相对更多数目的细胞生产该化合物。

上述用于增加谷氨酸棒状杆菌目标化合物产量的MP蛋白的诱变策略不用于限制；对这些诱变策略的变化对技术人员是显而易见的。本文公开的这些策略和机制使得可以使用本发明的核酸和蛋白质分子以产生表达突变的MP核酸和蛋白质分子的谷氨酸棒状杆菌或相关的细菌菌株，从而提高目标化合物的产量、生产率和/或产生效率。该目标化合物可为谷氨酸棒状杆菌的任一天然产物，其包括生物合成途径的终产物和天然代谢途径的中间体，该目标化合物也可为在谷氨酸棒状杆菌代谢中非天然存在的分子，但该分子为本发明的谷氨酸棒状杆菌菌株产生。

不应将下面的实施例理解为对限制对本发明的进一步阐述。在该申请中引用的所有参考文献、专利申请、专利、公开的专利申请、表的全部内容此处引用作为参考。

实施例

实施例1：谷氨酸棒状杆菌ATCC13032的总基因组DNA的制备

谷氨酸棒状杆菌(ATCC 13032)的培养物在30℃于BHI培养基(Difco)中剧烈振摇过夜。离心收获细胞，弃上清并将细胞重悬于5ml缓冲液-I(培养物原初体积的5％-所有陈述的体积按100ml培养物体积计算)。缓冲液-I的组成：140.34g/l蔗糖，2.46g/l MgSO₄·7H₂O，10ml/l KH₂PO₄溶液(100g/l，用KOH调节至pH 6.7)，50ml/l M12浓缩物(10g/l(NH₄)₂SO₄，1g/lNaCl，2g/l MgSO₄·7H₂O，0.2g/l CaCl₂，0.5g/l酵母提取物(Difco)，10ml/l痕量元素混合物(200mg/l FeSO₄·H₂O，10mg/l ZnSO₄·7H₂O，3mg/lMnCl₂·4H₂O，30mg/l H₃BO₃，20mg/l CoCl₂·6H₂O，1mg/l NiCl₂·6H₂O，3mg/l Na₂MoO₄·2H₂O，500mg/l络合剂(EDTA或柠檬酸)，100ml/l维生素-混合物(0.2mg/l生物素，0.2mg/l叶酸，20mg/l对氨基苯甲酸，20mg/l核黄素，40mg/l Ca-泛酸盐，140mg/l烟酸，40mg/l维生素B6盐酸盐，200mg/l肌醇)。向悬液中加入溶菌酶至终浓度2.5mg/ml。在37℃孵育约4小时后，细胞壁降解，离心收获所得的原生质体。沉淀用5ml缓冲液-I洗一次并用5ml TE-缓冲液(10mM Tris-HCl，1mM EDTA，pH 8)洗一次。将沉淀重悬于4ml TE-缓冲液，并加入0.5ml SDS溶液(10％)和0.5ml NaCl溶液(5M)。加入蛋白酶K至终浓度200μg/ml后，将悬液在37℃孵育约18小时。使用标准方法用酚、酚-氯仿-异戊醇和氯仿-异戊醇提取而纯化DNA。然后，加入1/50体积的3M醋酸钠和2体积的乙醇，于-20℃孵育30分钟，并在高速离心机上使用SS34转子(Sorvall)于12,000转/分离心30分钟，沉淀DNA。将该DNA溶于含20μg/ml RNaseA的1ml TE-缓冲液中，并用1000ml TE-缓冲液于4℃透析至少3小时。在此期间，更换缓冲液3次。向分装的0.4ml透析过的DNA溶液中加入0.4ml 2M LiCl和0.8ml乙醇。于-20℃孵育30分钟后，离心(13,000转/分，Biofuge Fresco，Heraeus，Hanau，德国)收集DNA。将该DNA沉淀溶于TE-缓冲液。用此方法制备的DNA可用于所有目的，包括Southern印迹或构建基因组文库。

实施例2：在大肠杆菌中构建谷氨酸棒状杆菌(ATCC13032)的基因组文库

从如实施例1所述制备的DNA开始，按照公知的且已建立的方法(参见例如，Sambrook，J.等人(1989)“分子克隆：实验室手册”(”MolecularCloning：A Laboratory Manual”)，Cold Spring Harbor Laboratory Press，或AUsubel，F.M.等人(1994)“分子生物学最新方法”(”Current Protocolsin Molecular Biology”)，John Wiley & Sons.)构建粘粒和质粒文库。

可使用任一质粒或粘粒。特别使用的是质粒pBR322(Sutcliffe，J.G.(1979)美国国家科学院院报(Proc.Natl.Acad.Sci.USA)，75：3737-3741)；pACYC177(Change & Cohen(1978)细菌学杂志(J.Bacteriol)134：1141-1156)、pBS系列的质粒(pBSSK+，pBSSK-及其它；Stratagene，LaJolla，USA)、或粘粒如SuperCos1(Stratagene，LaJolla，USA)或Lorist6(Gibson，T.J.，Rosenthal A.和Waterson，R.H.(1987)基因(Gene)53：283-286)。

实施例3：DNA序列测定和功能的计算分析

如实施例2所述的基因组文库按照标准方法用于DNA序列测定，尤其是使用ABI377序列测定仪通过链终止法测定(参见例如，Fleischman，R.D.等人(1995)”流感嗜血菌(Haemophilus Influenzae)Rd的全基因组随机序列测定和组装，科学(Science)，269：496-512)。使用具有以下核苷酸序列的测序引物：5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。

实施例4：体内诱变

谷氨酸棒状杆菌的体内诱变可通过将质粒(或其它载体)DNA在不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如Bacillus spp.或酵母如酿酒酵母)中传代而实施。常见的突变基因菌株在用于DNA修复系统的基因中有突变(例如mutHLS，mutD，mutT等；参考文献见大肠杆菌和沙门菌(Escherichia coli and Salmonella)，ASM：Washington一书中的Rupp，W.D.(1996)DNA修复机制，2277-2294页)。此类菌株为技术人员熟知。此类菌株的用途在例如Greener，A.和Callahan，M.(1994)Strategies 7：32-34中进行了阐述。

实施例5：在大肠杆菌和谷氨酸棒状杆菌之间的DNA转移

几种棒状杆菌和短杆菌种类含有自主复制的内源质粒(例如pHM1519或pBL1)(综述参见例如，Martin，J.F.等人(1987)生物技术(Biotechnology)，5：137-146)。使用标准的大肠杆菌载体易于构建大肠杆菌和谷氨酸棒状杆菌的穿梭载体(Sambrook，J.等人(1989)，“分子克隆：实验室手册”(”Molecular Cloning：A Laboratory Manual”)，Cold Spring HarborLaboratory Press，或Ausubel，F.M.等人(1994)“最新分子生物学方法”(”Current Protocols in Molecular Biology”)，John Wiley & Sons)，其中加入复制原点和来自谷氨酸棒状杆菌的适当标记。此类复制原点优选地取自从棒状杆菌和短杆菌分离的内源质粒。这些种类中作为转化标记尤其有用的是卡那霉素抗性基因(如衍生自Tn5或Tn903转座子的基因)或氯霉素抗性基因(Winnacker，E.L.(1987)”From Genes to Clones-Introductionto Gene Technology，VCH，Weinheim)。在文献中有许多在大肠杆菌和谷氨酸棒状杆菌中均复制的多种穿梭载体的例子，且其可用于多个目的，包括基因过表达(参考文献参见例如，Yoshihama，M.等人(1985)细菌学杂志(J.Bacteriol.)162：591-597，Martin J.F.等人(1987)生物技术(Biotechnology)，5：137-146和Eikmanns，B.J.等人(1992)基因(Gene)，102：93-98)。

使用标准技术，可将目的基因克隆入上述的穿梭载体之一并可将此杂合载体导入谷氨酸棒状杆菌菌株。谷氨酸棒状杆菌的转化可通过原生质体转化(Kastsumata，R.等人(1984)细菌畜杂志(J.Bacteriol.)159306-311)、电穿孔(Liebl，E.等人(1989)FEMS Microbiol.Letters，53：399-303)以及当使用特别的载体时，也通过接合(如在Sch_fer，A等人(1990)细菌学杂志(J.Bacteriol.)172：1663-1666中所述)来完成。也可转移谷氨酸棒状杆菌的穿梭载体至大肠杆菌，其为通过制备来自谷氨酸棒状杆菌的质粒DNA(使用本领域公知的标准方法)并将其转化至大肠杆菌。该转化步骤可使用标准方法进行，但有利的是使用Mcr-缺陷的大肠杆菌菌株，如NM522(Gough & Murray(1983)分子生物学杂志(J.Mol.Biol.)166：1-19)。

实施例6：突变蛋白质的表达的确定

在转化的宿主细胞中对突变蛋白质活性的观察依赖于突变蛋白质以与野生型蛋白质相似的方式和相似的量表达的事实。确定突变基因转录水平(指示可用于翻译为基因产物的mRNA量)的有用方法是进行Northern印迹(参考文献见例如，Ausubel等人(1988)分子生物学最新方法(CurrentProtocols in Molecular Biology)，Wiley：New York)，其中设计为与目的基因结合的引物用可检测标志(通常为放射性的或化学发光的)标记，这样当提取生物体培养物的总RNA、在凝胶上运行、转移至稳定的基质并与该探针孵育时，探针的结合和结合量指示了该基因的存在和其mRNA量。该信息是突变基因转录程度的证据。通过几种方法可从谷氨酸棒状杆菌中分离总细胞RNA，这些方法均为本领域熟知，如在Bormann，E.R.等人(1992)分子微生物学(Mol.Microbiol.)6：317-326中所描述。

为了确定从该mRNA翻译的蛋白质的存在或相对量，可使用标准技术如Western印迹(参见例如，Ausubel等人(1988)分子生物学最新方法(Current Protocols in Molecular Biology)，Wiley：New York)。在该方法中，提取细胞总蛋白质、通过凝胶电泳分离、转移至基质如硝化纤维并与探针孵育，其中所述探针如与目标蛋白质特异性结合的抗体。该探针通常用易于检测到的化学发光或显色标记物标记。所观察到的标记物的存在和量指示了在细胞中目标突变蛋白质的存在和量。

实施例7：遗传改变的谷氨酸棒状杆菌的生长-培养基和培养条件

遗传修饰的棒状杆菌培养在合成或天然的生长培养基中。用于棒状杆菌的许多种不同生长培养基为人们熟知并易于获得(Lieb等人(1989)Appl.Microbiol.Biotechnol.，32：205-210；von der Osten等人(1998)Biotechnology Letters，11：11-16；Patent DE 4,120,867；在原核生物(TheProcaryotes)，Volume II，Balows，A.等人编辑.Springer-Verlag一书中的Liebl(1992)“棒状杆菌属”(“The Genus Corynebacteria”))。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源是糖类，如单糖、二糖或或多糖。优良碳源的实例是葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可通过复合化合物如糖蜜或其它来自糖加工的副产品提供糖至培养基。提供不同碳源的混合物也可是有利的。其它可能的碳源为醇类和有机酸，如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物，或为包含这些化合物的物质。示例性氮源包括氨气或胺盐如NH₄Cl或(NH₄)₂SO₄、NH₄OH、硝酸盐、尿素、氨基酸和复合氮源如玉米浆、大豆粉、大豆蛋白质、酵母提取物、肉羹等。

可包括在培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、亚磷酸盐或硫酸盐。可向培养基中加入螯合物以维持溶液中的金属离子。尤其有用的螯合物包括二羟基酚，如儿茶酚或原儿茶酚盐，和有机酸如柠檬酸。培养基一般也包括其它生长因子如维生素或生长促进剂，其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和维生素B₆。生长因子和盐通常来自复合的培养基组成，如酵母提取物、糖蜜、玉米浆等。培养基化合物的精确组成强烈取决于直接实验并在每一具体情况下单独确定。有关培养基优化的信息在课本“Applied Microbiol.Physiology，A Practical Approach”(P.M.Rhodes，P.F.Stanbury编辑，IRL Press(1997)53-73页，ISBN 0 19 963577 3)中可找到。也可从供应商处得到生长培养基，如Standard 1(Merck)或BHI(grain heart infusion，DIFCO)和其它。

通过加热(1.5巴，121℃加热20分钟)或过滤除菌对所有的培养基组成成份进行灭菌。组成成份可一起灭菌，或需要的话分开灭菌。所有的培养基组成成份可在培养开始时存在，或它们可任选地连续加入或分批加入。

对每一实验分别定义培养条件。温度应在15℃和45℃的范围之间。在实验中温度可保持恒定或可改变。培养基的pH应在5至8.5的范围之间，优选地在7.0附近，可通过向培养基中添加缓冲液而维持。用于此目的的示例性缓冲液为磷酸钾缓冲液。可备选地或同时使用合成缓冲液如MOPS、HEPES、ACES和其它。在生长过程中通过加入NaOH或NH₄OH也可维持恒定的培养pH。如果使用复合的培养基组成如酵母提取物，可减轻对额外的缓冲液的需要，因为许多复合化合物具有高缓冲能力。如果使用发酵罐培养微生物，用气态氨也可控制pH。

培养时间通常在几小时到几天之间。选择培养时间以允许肉汤中累积最大量的产物。可在多种容器如不同规格的微滴定板、玻璃管、玻璃烧瓶或玻璃或金属发酵罐中实施公开的生长实验。对于筛选大量的克隆，应在微滴定板、玻璃管或在有或没有挡板的摇瓶中培养微生物。优选地使用100ml摇瓶，其中装有10％(体积)的所需生长培养基。该烧瓶应在旋转振荡器(振幅25mm)上以100-300转/分摇动。可通过维持潮湿环境减少蒸发损失；备选地，对蒸发损失应进行数学上的校正。

如果试验遗传修饰的克隆，也应试验未修饰的对照克隆或含基本质粒的无任何插入的对照克。使用已于30℃孵育的在琼脂板上生长的细胞接种培养基至OD₆₀₀ 0.5-1.5，其中所述琼脂板如CM板(10g/l葡萄糖，2.5g/lNaCl，2g/l尿素，10g/l聚胨，5g/l酵母抽取物，5g/l肉羹，22g/l琼脂，用2M NaOH调节至pH 6.8)。培养基的接种通过导入来自CM板的谷氨酸棒状杆菌细胞的盐水悬液或添加该细菌的原液体培养物而完成。

实施例8：突变蛋白质功能的体外分析

本领域已建立了对酶活性和动力学的确定。确定特定的改变酶的活性的实验应加以修改，使适应野生型酶特异活性的确定，这在本领域普通技术人员的能力范围内。可在下列参考文献中找到对酶从总体上的概观，以及有关结构、动力学、原理、方法、应用和对许多酶活性进行确定的例子的具体说明，例如Dixon，M.和Webb，E.C.，(1979)酶(Enzymes).Longmans：伦敦；Fersht，(1985)酶结构和机制(Enzyme Structure andMechanism).Freeman：纽约；Walsh，(1979)酶促反应机制(EnzymaticReaction Mechanisms).Freeman：San Francisco；Price，N.C.，Stevens，L.(1982)酶学基础(Fundamentals of Enzymology).Oxford Univ.Press：Oxford；Boyer，P.D.编辑(1983)酶(The Enzymes)，第三版，Academic Press：纽约；Bisswanger，H.，(1994)酶动力学(Enzymkinetik)，第二版.VCH：Weinheim(ISBN 3527300325)；Bergmeyer，H.U.，Bergmeyer，J.，Graβl，M.编辑(1983-1986)酶促分析方法(Methods of Enzymatic Analysis)，第三版，I-XII卷，Verlag Chemie：Weinheim；以及Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)(1987)A9卷，“酶”.VCH：Weinheim，352-363页。

可通过几种已建立的方法测定结合DNA的蛋白质的活性，如DNA带移动试验(也称为凝胶阻滞试验)。此类蛋白质对其它分子表达的影响可使用报告基因试验测定(如在Kolmar，H.等人(1995)EMBO J.14：3895-3904及其中所引用的参考文献中所述)。报告基因测试系统为人们所熟知，并被建立以应用于原核和真核细胞，使用的酶如β-半乳糖苷酶、绿色荧光蛋白和几种其它的酶。

可根据如在生物膜，分子结构和功能(Biomembranes，MolecularStructure and Function)，Springer：Heidelberg一书中的Gennis，R.B.(1989)”Pores，Channels and Transporters”，85-137；199-234和270-322页中所述的技术进行膜转运蛋白活性的测定。

实施例9：分析突变蛋白质对目标产物产生的影响

谷氨酸棒状杆菌中的遗传修饰对目标化合物(如氨基酸)产生的影响可通过将经修饰的微生物在合适的条件(如上文中所描述的那些条件)下生长并分析用于增加目标产物(即氨基酸)产生的培养基和/或细胞组成来确定。此类分析技术为技术人员熟知，包括光谱学、薄层色谱法、多种染色法、酶促和微生物学方法、以及分析色谱法如高效液相色谱法(参见例如，Ullman工业化学百科全书(Ullman，Encyclopedia of IndustrialChemistry)，A2卷，89-90和443-613页，VCH：Weinheim(1985)；Fallon，A.等人(1987)生物化学和分子生物学实验室技术(Laboratory Techniques inBiochemistry and Molecular Biology)中的“HPLC在生物化学中的应用”(”Applications of HPLC in Biochemistry”)，17卷；Rehm等人(1993)生物技术(iotechnology)，3卷，III章“产物回收和纯化”(”Product recoveryand purification”)，469-714页，VCH：Weinheim；Belter，P.A.等人(1988)生物分离：生物技术中的下游加工(Bioseparations：downstream processingfor biotechnology)，John Wiley and Sons；Kennedy，J.F.和Cabral，J.M.S.(1992)生物物质的回收方法(Recovery processes for biological materials)John Wiley and Sons；在Ulmann工业化学百科全书(Ulmann’sEncyclopedia of Industrial Chemistry)一书中的Shaeiwitz，J.A.和Henry，J.D.(1988)生物化学分离(Biochemical separations)，B3卷，11章，1-27页，VCH：Weinheim；以及Dechow，F.J.(1989)生物技术中的分离和纯化技术(Separation and purification techniques in biotechnology)，NoyesPublications.)。

除了测定发酵终产物外，还可分析用于产生目标化合物的代谢途径的其它组成如中间体和副产物来确定化合物的总体产生力、化合物的产率和/或生产效率。分析方法包括测定培养基中的营养素水平(例如，糖类、碳水化合物、氮源、磷酸盐和其它离子)、测定生物量组成和生长、分析生物合成途径中共同代谢物的产生及测定发酵过程中产生的气体。这些测定的标准方法在应用微生物生理学，实用方法(Applied Microbial Physiology，APractical Approach)，P.M.Rhodes和P.F.Stanbury编辑，IRI Press，103-129；131-163和165-192页(ISBN：0199635773)及其引用的参考文献中列出。

实施例10：从谷氨酸棒状杆菌培养物中纯化目标产物

可通过多种本领域熟知的方法实施从谷氨酸棒状杆菌细胞或上述培养物上清回收目标产物。如果目标产物不从细胞中分泌，可低速离心从培养物中收获细胞、用标准技术如机械力或超声裂解细胞。离心去除细胞碎片，保留含有可溶蛋白质的上清级分用于进一步纯化目标化合物。如果化合物从谷氨酸棒状杆菌细胞分泌，则低速离心从培养物中去除细胞，并保留上清级分用于进一步纯化。

将来自两种中任一纯化方法的上清级分用合适的树脂进行层析，其中目标分子保留在层析树脂上而样本中的许多杂质不保留在层析树脂上，或者杂质保留在层析树脂上而样本不保留。此层析步骤必需时可重复，其中使用相同或不同的层析树脂。技术人员在选择合适的层析树脂以及将它们最有效地用于特定的待纯化分子方面是很精通的。可通过过滤或超滤浓缩纯化产物，并贮存在使产物稳定性最高的温度下。

有大量的纯化方法为本领域公知，上述纯化方法不用于限制。此类纯化技术例如在Bailey，J.E.和Ollis，D.F.生物化学工程基础(BiochemicalEngineering Fundamentals)，McGraw-Hill：纽约(1986)中描述。

分离化合物的特性和纯度可通过本领域的标准技术确定。这些技术包括高效液相色谱(HPLC)、光谱法、染色法、薄层色谱法、NIRS、酶促试验或微生物学方法。此类分析方法在Patek等人(1994)Appl.Environ.Microbiol.60：133-140；Malakhova等人(1996)生物技术(Biotekhnologiya)11：27-32；和Schmidt等人(1998)生物加工工程(Bioprocess Engineer)19：67-70.Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)(1996)A27卷，VCH：Weinheim，89-90页，521-540页，540-547页，559-566页，575-581页和581-587页；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，John Wiley and Sons；生物化学和分子生物学中的实验室技术(Laboratory Techniques in Biochemistry and Molecular Biology)，17卷中的Fallon，A.等人(1987)HPLC在生物化学中的应用(Applications ofHPLC in Biochemistry)。

等同物

技术人员知道或通过简单的常规方法可确定许多于文中所描述本发明具体实施方案的等同物。此类等同物旨在包括在下面的专利权利要求书中。

表1中的信息理解如下：

在第1列中，“DNA ID”，每种情况下相关数字指所公开序列表的SEQ ID NO。所以，“DNA ID”列中的“5”指SEQ ID NO：5。

在第2列中，“AA ID”，每种情况下相关数字指所公开序列的SEQ IDNO。“AA ID”列中的“6”指SEQ ID NO：6。

在第3列中，列出了“标识”——每个序列的明确内部名称。

在第4列中，“AA pos”，每种情况下相关数字指相同行中多肽序列“AA ID”的氨基酸位置。所以，“AA pos”列中“26”是相应所示的多肽序列的氨基酸位置26。位置计算从N末端的+1开始。

在第5列中，“AA野生型”，每种情况下相关数字指相应野生型菌株在第4列中所示位置的氨基酸(用单字母码)。

在第6列中，“AA突变株”，每种情况下相关数字指相应突变菌株在第4列中所示位置的氨基酸(用单字母码)。

在第7列中，列出了“功能”——相应多肽序列的生理学功能。

蛋白原氨基酸的单字母代码：

A 丙氨酸

C 半胱氨酸

D 天冬氨酸

E 谷氨酸

F 苯丙氨酸

G 甘氨酸

H 组氨酸

I 异亮氨酸

K 赖氨酸

L 亮氨酸

M 甲硫氨酸

N 天冬酰胺

P 脯氨酸

Q 谷氨酰胺

R 精氨酸

S 丝氨酸

T 苏氨酸

V 缬氨酸

W 色氨酸

Y 酪氨酸

表1

编码调节蛋白的基因

DNA ID： AA ID：标识 AA位置： AA野生型 AA突变体功能：

1 2 RXA00116 26 L F 天冬氨酸转氨酶(EC 2.6.1.1)

3 4 RXA00147 144 I L 氨甲酰磷酸合酶小链(EC 6.3.5.5)

239 G S 氨甲酰磷酸合酶小链(EC 6.3.5.5)

5 6 RXA00152 96 P S STOMATIN样蛋白

237 G S STOMATIN样蛋白

7 8 RXA00166 103 T I 甲基转移酶(EC 2.1.1.-)

9 10 RXA00278 144 G D 谷氨酰胺结合蛋白前体

11 12 RXA00323 41 A V 谷氨酰胺合成酶(EC 6.3.1.2)

80 G E 谷氨酰胺合成酶(EC 6.3.1.2)

13 14 RXA00330 492 T I 丝氨酸合酶(EC 4.2.99.2)

15 16 RXA00383 57 A T 原卟啉原氧化酶(EC 1.3.3.4)

17 18 RXA00389 55 A T 酮戊二酸半醛脱氢酶(EC 1.2.1-)

481 G E 酮戊二酸半醛脱氢酶(EC 1.2.1-)

19 20 RXA00416 223 A V 推定的跨膜蛋白

21 22 RXA00489 338 S F GMP还原酶(EC 1.6.6.8)

23 24 RXA00552 295 A T 硫代硫酸转硫酶(EC 2.8.1.1)

25 26 RXA00579 490 P L 对氨基苯甲酸合酶组分1(EC 4.1.3.-)

27 28 RXA00620 141 G D 氨基咪唑核苷酸-琥珀酰基羧酰胺合酶(EC 6.3.2.6)

29 30 RXA00636 146 E K 结合FAD的氧化还原酶

31 32 RXA00708 256 G S 2，5-二酮-D-古洛糖酸还原酶(EC 1.1.1.-)

33 34 RXA00727 78 P S 谷氨酰胺-结合周质蛋白前体

149 A V 谷氨酰胺-结合周质蛋白前体

280 A V 谷氨酰胺-结合周质蛋白前体

35 36 RXA00867 513 P S 多核糖核苷酸核苷酸基转移酶(EC 2.7.7.8)

37 38 RXA00955 180 A V 吲哚-3-甘油磷酸合酶(EC 4.1.1.48)/

N-(5′-磷酸-核糖基)邻氨基苯甲酸异构酶(EC 5.3.1.24)

490 L F 吲哚-3-甘油磷酸合酶(EC 4.1.1.48)/

N-(5′-磷酸-核糖基)邻氨基苯甲酸异构酶(EC 5.3.1.24)

39 40 RXA00957 220 P L 邻氨基苯甲酸合酶组分I(EC 4.1.3.27)

41 42 RXA00970 133 A V 高丝氨酸激酶(EC 2.7.1.39)

138 P S 高丝氨酸激酶(EC 2.7.1.39)

43 44 RXA00997 117 A V 3-去甲基泛醌-9 3-甲基转移酶(EC 2.1.1.64)

45 46 RXA01095 262 P S 吲哚-3-甘油磷酸合酶(EC 4.1.1.48)

47 48 RXA01105 270 A V 组氨醇-磷酸氨基转移酶(EC 2.6.1.9)

49 50 RXA01115 72 T I 3-氧基己二酸烯醇-内酯水解酶(EC 3.1.1.24)/

4-羧基粘康酸内酯脱羧酶(EC 4.1.1.44)

51 52 RXA01127 60 D N 3-异丙基苹果酸脱氢酶(EC 1.1.1.85)

53 54 RXA01240 23 A T GTP磷酸激酶(EC 2.7.6.5)

55 56 RXA01253 112 A T 钴啉胺酸合酶(EC 3.-.-.-)

57 58 RXA01321 156 V A 甲酰四氢叶酸脱甲酰酶(EC 3.5.1.10)

59 60 RXA01381 180 G D THIF蛋白

61 62 RXA01434 957 S F 毒力因子MVIN

1012 P S 毒力因子MVIN

63 64 RXA01442 122 R H 磷酸核糖基甘氨酰胺甲酰基转移酶2(EC 2.1.2.-)

65 66 RXA01483 200 R C 脱氧鸟苷三磷酸三磷酸水解酶(EC 3.1.5.1)

326 P S 脱氧鸟苷三磷酸三磷酸水解酶(EC 3.1.5.1)

67 68 RXA01515 239 A V 二氢蝶啉酯合酶(EC 2.5.1.15)

69 70 RXA01518 148 A T 推定的的跨膜蛋白

71 72 RXA01690 184 G D 支链氨基酸转氨酶(EC 2.6.1.42)

73 74 RXA01746 146 G E 硫辛酸-蛋白连接酶B(EC 6.-.-.-)

75 76 RXA01810 263 G S 氯高铁血红素-结合周质蛋白HMUT前体

77 78 RXA01850 1 V M L-丝氨酸脱水酶(EC 4.2.1.13)

45 S N L-丝氨酸脱水酶(EC 4.2.1.13)

79 80 RXA01940 269 A V

81 82 RXA02021 305 S F 2，3，4，5-四氢吡啶-2-羧酸N-琥珀酰转移酶(同源物)

83 84 RXA02095 409 A V ABC转运蛋白ATP-结合蛋白

914 A V ABC转运蛋白ATP-结合蛋白

85 86 RXA02156 48 A T 乙酰谷氨酸激酶(EC 2.7.2.8)

87 88 RXA02162 434 T I 精氨酸琥珀酸裂解酶(EC 4.3.2.1)

89 90 RXA02176 136 P S 磷酸丝氨酸转氨酶(EC 2.6.1.52)

91 92 RXA02194 129 L F ATP磷酸核糖基转移酶(EC 2.4.2.17)

93 94 RXA02206 73 G D 氧化还原酶(EC 1.1.1.-)

114 A T 氧化还原酶(EC 1.1.1.-)

314 R C 氧化还原酶(EC 1.1.1.-)

95 96 RXA02237 78 D N 鸟苷酸激酶(EC 2.7.4.8)

97 98 RXA02239 168 P S 磷酸泛酸-半胱氨酸连接酶(EC 6.3.2.5)/磷酸

泛酸基半胱氨酸脱羧酶(EC 4.1.1.36)

99 100 RXA02250 24 P S RIBX蛋白

101 102 RXA02295 130 G D 转运蛋白

103 104 RXA02299 36 A V 天冬氨酸1脱羧酶(EC 4.1.1.11)

105 106 RXA02382 416 E K γ-谷酰基磷酸还原酶(GPR)(EC 1.2.1.41)

107 108 RXA02390 195 G D 苏氨酸流出蛋白

109 110 RXA02499 65 G S 吡咯啉-5-羧酸还原酶(EC 1.5.1.2)

216 A T 吡咯啉-5-羧酸还原酶(EC 1.5.1.2)

111 112 RXA02516 158 P S 半胱氨酸脱硫水化酶(EC 4.4.1.-)/硒代半胱氨酸裂合酶(EC 4.4.1.16)

113 114 RXA02532 284 P S 胱硫醚γ-合酶(EC 4.2.99.9)

115 116 RXA02536 132 P S 碳-氮水解酶

117 118 RXA02560 138 G E 氧不敏感的NAD(P)H氮还原酶(EC 1.-.-.-)/二氢蝶啶还原酶(EC 1.6.99.7)

192 P S 氧不敏感的NAD(P)H氮还原酶(EC 1.-.-.-)/二氢蝶啶还原酶(EC 1.6.99.7)

119 120 RXA02653 448 G E 依赖吡哆醛的脱羧酶

121 122 RXA02754 374 P S 烟酰酸磷酸核糖基转移酶(EC 2.4.2.11)

123 124 RXA02758 110 G E 磷酸丝氨酸磷酸酶(EC 3.1.3.3)

125 126 RXA02790 30 L F 4-氨基-4-脱氧分支酸裂合酶(EC 4.-.-.-)

127 128 RXA02912 62 G D 甲基转移酶(EC 2.1.1.-)

129 130 RXA02970 233 E K 转氨酶

131 132 RXA03003 209 P L 天冬氨酸转氨酶(EC 2.6.1.1)

133 134 RXA03171 70 P S 尿嘧啶磷酸核糖基转移酶(EC 2.4.2.9)

144 L F 尿嘧啶磷酸核糖基转移酶(EC 2.4.2.9)

199 A V 尿嘧啶磷酸核糖基转移酶(EC 2.4.2.9)

135 136 RXA03282 312 G D 胱硫醚γ-合酶(EC 4.2.99.9)

137 138 RXA03465 224 P S 钼辅因子生物合成蛋白A

261 P S 钼辅因子生物合成蛋白A

139 140 RXA03677 389 A T 海藻糖/麦芽糖结合蛋白

141 142 RXA03695 70 T I 甲基转移酶(EC 2.1.1.-)

143 144 RXA03754 459 G D 3-异丙基苹果酸脱氢酶大亚基(EC 4.2.1.33)

145 146 RXA03829 36 A T 可能的乙酰乳酸合酶家族

147 148 RXA03871 33 R H 钼蝶呤生物合成的MOEA蛋白

360 T I 钼蝶呤生物合成的MOEA蛋白

149 150 RXA04120 348 G D γ-谷氨酰基转肽酶(EC 2.3.2.2)

151 152 RXA04173 41 T I GMP合酶-谷氨酸氨基转移酶结构域

153 154 RXA04174 856 G S 乌头酸水合酶(EC 4.2.1.3)

155 156 RXA04186 385 A T 5-甲基四氢叶酸--高半胱氨酸甲基转移酶(EC 2.1.1.13)

492 V I 5-甲基四氢叶酸--高半胱氨酸甲基转移酶(EC 2.1.1.13)

990 S N 5-甲基四氢叶酸--高半胱氨酸甲基转移酶(EC 2.1.1.13)

157 158 RXA04228 279 A T 谷氨酰胺酶(EC 3.5.1.2)

159 160 RXA04360 161 P S 邻氨基苯甲酸磷酸核糖基转移酶(EC 2.4.2.18)

161 162 RXA07000 398 P S 谷氨酸合酶[NADPH]大链(EC 1.4.1.13)

163 164 RXA07001 110 A T 麦芽糖结合蛋白前体

序列表

<110>巴斯福股份公司

<120>编码代谢途径蛋白的基因

<130>O.Z.0050/52968

<160>164

<210>1

<211>1339

<212>DNA

<213>谷氨酸棒状杆菌(Corynebacterium glutamicum)

<220>

<221>CDS

<222>(101)..(1309)

<223>RXA00116

<400>1

gcaccctctc tttttttgtg tttgtggggg tcttgggccc cttgtgcacg cggcacgcac 60

gctgggggca agcgtcgaca agcacaaact ttttgcttaa ttg aat cct ttg cgc 115

Leu Asn Pro Leu Arg

1 5

acc aat caa tgg ggg atc aaa tat agt agc tgc atg agt aat gac ttc 163

Thr Asn Gln Trp Gly Ile Lys Tyr Ser Ser Cys Met Ser Asn Asp Phe

10 15 20

gtc gtt tct agg ctt aga ccc ttt ggt gaa acg att ttt gca acc atg 211

Val Val Ser Arg Leu Arg Pro Phe Gly Glu Thr Ile Phe Ala Thr Met

25 30 35

acc cag cga gct gtt gag gcg ggt gca atc aat ctt ggt cag ggc ttt 259

Thr Gln Arg Ala Val Glu Ala Gly Ala Ile Asn Leu Gly Gln Gly Phe

40 45 50

cct gat gag gat ggt cct cgt cgg atg tta gag atc gcg tcg gag cag 307

Pro Asp Glu Asp Gly Pro Arg Arg Met Leu Glu Ile Ala Ser Glu Gln

55 60 65

att ctc ggg gga aat aat cag tat tcg gcg ggg cgt ggg gat gct tcg 355

Ile Leu Gly Gly Asn Asn Gln Tyr Ser Ala Gly Arg Gly Asp Ala Ser

70 75 80 85

ttg agg gca gct gtg gct cgt gat cat ttg gag agg ttt gat ctg gag 403

Leu Arg Ala Ala Val Ala Arg Asp His Leu Glu Arg Phe Asp Leu Glu

90 95 100

tac aac cct gat tcg gag gtg ttg atc acg gtg ggg gcc act gag gcg 451

Tyr Asn Pro Asp Ser Glu Val Leu Ile Thr Val Gly Ala Thr Glu Ala

105 110 115

att acg gcg act gtg ttg ggt ttg gtg gag cct ggg gat gaa gtg atc 499

Ile Thr Ala Thr Val Leu Gly Leu Val Glu Pro Gly Asp Glu Val Ile

120 125 130

gtt ttg gaa ccg tat tac gat gcg tat gcg gcg gct att gcg ttg gcg 547

Val Leu Glu Pro Tyr Tyr Asp Ala Tyr Ala Ala Ala Ile Ala Leu Ala

135 140 145

ggg gcg acg cgg gtg gcg gtt cct ttg cag gag gtg gag aac tcg tgg 595

Gly Ala Thr Arg Val Ala Val Pro Leu Gln Glu Val Glu Asn Ser Trp

150 155 160 165

gat gtg gat gtc gat aag ttg cat gcg gcg gtg act aag aag acg cgg 643

Asp Val Asp Val Asp Lys Leu His Ala Ala Val Thr Lys Lys Thr Arg

170 175 180

atg att atc gtt aat tcg ccg cat aat ccg acg ggt tcg gtg ttt tct 691

Met Ile Ile Val Asn Ser Pro His Asn Pro Thr Gly Ser Val Phe Ser

185 190 195

aag aag gcg ttg aag cag ttg gcg ggt gtt gct cgt gcg tat gac ttg 739

Lys Lys Ala Leu Lys Gln Leu Ala Gly Val Ala Arg Ala Tyr Asp Leu

200 205 210

ttg gtg ttg tca gat gag gtg tat gag cat ctt gtt ttt gat gat cag 787

Leu Val Leu Ser Asp Glu Val Tyr Glu His Leu Val Phe Asp Asp Gln

215 220 225

aag cat gtg agt gtc gcg aag ctg ccc ggt atg tgg gat cgc acg gtg 835

Lys His Val Ser Val Ala Lys Leu Pro Gly Met Trp Asp Arg Thr Val

230 235 240 245

acg gtg tcg tcg gcg gcg aaa acg ttc aat gtg act ggt tgg aag acg 883

Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val Thr Gly Trp Lys Thr

250 255 260

ggg tgg gcg ttg gca ccg gag ccg ttg ttg gag gcg gtg ttg aag gcg 931

Gly Trp Ala Leu Ala Pro Glu Pro Leu Leu Glu Ala Val Leu Lys Ala

265 270 275

aag cag ttt atg tct tat gtg ggg gct aca cct ttt cag ccg gct gtg 979

Lys Gln Phe Met Ser Tyr Val Gly Ala Thr Pro Phe Gln Pro Ala Val

280 285 290

gcg cat gcg att gaa cat gag cag aag tgg gtg tca aag atg tct aag 1027

Ala His Ala Ile Glu His Glu Gln Lys Trp Val Ser Lys Met Ser Lys

295 300 305

ggg ctt gag ctc aag cgg gat att ttg cgt act gcg tta gat aag gcg 1075

Gly Leu Glu Leu Lys Arg Asp Ile Leu Arg Thr Ala Leu Asp Lys Ala

310 315 320 325

ggg ctg aag act cat gac agt atg ggc acg tat ttc atc gtt gcg gat 1123

Gly Leu Lys Thr His Asp Ser Met Gly Thr Tyr Phe Ile Val Ala Asp

330 335 340

att ggg gat cgt gat ggt gcg gag ttc tgt ttt gag ttg att gag aag 1171

Ile Gly Asp Arg Asp Gly Ala Glu Phe Cys Phe Glu Leu Ile Glu Lys

345 350 355

gtt ggg gtg gcg gcg att ccg gtg cag gcg ttt gtg gat cat ccg aag 1219

Val Gly Val Ala Ala Ile Pro Val Gln Ala Phe Val Asp His Pro Lys

360 365 370

aag tgg tcg tcg aag gtt cgt ttt gcg ttt tgc aaa aaa gaa gag acg 1267

Lys Trp Ser Ser Lys Val Arg Phe Ala Phe Cys Lys Lys Glu Glu Thr

375 380 385

ctc cgc gaa gct gcg gag cgt ctc aag ggg att aag aaa cta 1309

Leu Arg Glu Ala Ala Glu Arg Leu Lys Gly Ile Lys Lys Leu

390 395 400

tagtttgaac aggttgttgg gggtcttttt 1339

<210>2

<211>403

<212>PRT

<213>谷氨酸棒状杆菌

<400>2

Leu Asn Pro Leu Arg Thr Asn Gln Trp Gly Ile Lys Tyr Ser Ser Cys

1 5 10 15

Met Ser Asn Asp Phe Val Val Ser Arg Leu Arg Pro Phe Gly Glu Thr

20 25 30

Ile Phe Ala Thr Met Thr Gln Arg Ala Val Glu Ala Gly Ala Ile Asn

35 40 45

Leu Gly Gln Gly Phe Pro Asp Glu Asp Gly Pro Arg Arg Met Leu Glu

50 55 60

Ile Ala Ser Glu Gln Ile Leu Gly Gly Asn Asn Gln Tyr Ser Ala Gly

65 70 75 80

Arg Gly Asp Ala Ser Leu Arg Ala Ala Val Ala Arg Asp His Leu Glu

85 90 95

Arg Phe Asp Leu Glu Tyr Asn Pro Asp Ser Glu Val Leu Ile Thr Val

100 105 110

Gly Ala Thr Glu Ala Ile Thr Ala Thr Val Leu Gly Leu Val Glu Pro

115 120 125

Gly Asp Glu Val Ile Val Leu Glu Pro Tyr Tyr Asp Ala Tyr Ala Ala

130 135 140

Ala Ile Ala Leu Ala Gly Ala Thr Arg Val Ala Val Pro Leu Gln Glu

145 150 155 160

Val Glu Asn Ser Trp Asp Val Asp Val Asp Lys Leu His Ala Ala Val

165 170 175

Thr Lys Lys Thr Arg Met Ile Ile Val Asn Ser Pro His Asn Pro Thr

180 185 190

Gly Ser Val Phe Ser Lys Lys Ala Leu Lys Gln Leu Ala Gly Val Ala

195 200 205

Arg Ala Tyr Asp Leu Leu Val Leu Ser Asp Glu Val Tyr Glu His Leu

210 215 220

Val Phe Asp Asp Gln Lys His Val Ser Val Ala Lys Leu Pro Gly Met

225 230 235 240

Trp Asp Arg Thr Val Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val

245 250 255

Thr Gly Trp Lys Thr Gly Trp Ala Leu Ala Pro Glu Pro Leu Leu Glu

260 265 270

Ala Val Leu Lys Ala Lys Gln Phe Met Ser Tyr Val Gly Ala Thr Pro

275 280 285

Phe Gln Pro Ala Val Ala His Ala Ile Glu His Glu Gln Lys Trp Val

290 295 300

Ser Lys Met Ser Lys Gly Leu Glu Leu Lys Arg Asp Ile Leu Arg Thr

305 310 315 320

Ala Leu Asp Lys Ala Gly Leu Lys Thr His Asp Ser Met Gly Thr Tyr

325 330 335

Phe Ile Val Ala Asp Ile Gly Asp Arg Asp Gly Ala Glu Phe Cys Phe

340 345 350

Glu Leu Ile Glu Lys Val Gly Val Ala Ala Ile Pro Val Gln Ala Phe

355 360 365

Val Asp His Pro Lys Lys Trp Ser Ser Lys Val Arg Phe Ala Phe Cys

370 375 380

Lys Lys Glu Glu Thr Leu Arg Glu Ala Ala Glu Arg Leu Lys Gly Ile

385 390 395 400

Lys Lys Leu

<210>3

<211>1309

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1279)

<223>RXA00147

<400>3

attgcatata atgcaatgaa ttgaataaac tacattcagg gttatcaacc agccaatttc 60

ttttaaaaag gcagacacac gaaaggcgac aacagtcacc gtg agt aaa gac acc 115

Val Ser Lys Asp Thr

1 5

acc acc tac cag gga gtc acc gag atc gga tcc gtt ccg gca tac ctg 163

Thr Thr Tyr Gln Gly Val Thr Glu Ile Gly Ser Val Pro Ala Tyr Leu

10 15 20

gtt ctt gca gac gga cgt acc ttc acc gga ttt ggc ttt gga gct atc 211

Val Leu Ala Asp Gly Arg Thr Phe Thr Gly Phe Gly Phe Gly Ala Ile

25 30 35

ggc acc acc ctt ggt gag gca gtg ttc act acc gcc atg acc ggt tac 259

Gly Thr Thr Leu Gly Glu Ala Val Phe Thr Thr Ala Met Thr Gly Tyr

40 45 50

caa gaa acc atg acc gat cct tcc tat cac cgc cag att gtt gtg gct 307

Gln Glu Thr Met Thr Asp Pro Ser Tyr His Arg Gln Ile Val Val Ala

55 60 65

acc gca cca cag atc ggc aac acc ggc tgg aac gat gag gac aac gag 355

Thr Ala Pro Gln Ile Gly Asn Thr Gly Trp Asn Asp Glu Asp Asn Glu

70 75 80 85

tcc cgc gac ggc aag att tgg gtt gca ggc ctt gtt atc cgc gac ctc 403

Ser Arg Asp Gly Lys Ile Trp Val Ala Gly Leu Val Ile Arg Asp Leu

90 95 100

gca gca cgt gtg tcc aac tgg cgc gcc acc acc tcc ttg cag cag gaa 451

Ala Ala Arg Val Ser Asn Trp Arg Ala Thr Thr Ser Leu Gln Gln Glu

105 110 115

atg gca ggc cag ggc atc gtc ggc atc ggc gga atc gac acc cgc gca 499

Met Ala Gly Gln Gly Ile Val Gly Ile Gly Gly Ile Asp Thr Arg Ala

120 125 130

ctg gtt cgc cac ctg cgc aat gaa ggt tcc att gca gcg ggc atc ttc 547

Leu Val Arg His Leu Arg Asn Glu Gly Ser Ile Ala Ala Gly Ile Phe

135 140 145

tcc ggc gct gac gca cag cgc cca gtt gaa gaa ctc gta gag atc gtc 595

Ser Gly Ala Asp Ala Gln Arg Pro Val Glu Glu Leu Val Glu Ile Val

150 155 160 165

aag aat cag cca gca atg acc ggc gca aac ctc tcc gtt gag gtc tct 643

Lys Asn Gln Pro Ala Met Thr Gly Ala Asn Leu Ser Val Glu Val Ser

170 175 180

gct gat gaa acc tac gtc atc gaa gct gaa ggc gaa gag cgc cac acc 691

Ala Asp Glu Thr Tyr Val Ile Glu Ala Glu Gly Glu Glu Arg His Thr

185 190 195

gtc gtg gcc tac gac ctg ggc att aag caa aac acc cca cgt cgt ttc 739

Val Val Ala Tyr Asp Leu Gly Ile Lys Gln Asn Thr Pro Arg Arg Phe

200 205 210

tct gca cgc ggt gtt cgc acc gtc atc gtg cct gct gaa acc cca ttc 787

Ser Ala Arg Gly Val Arg Thr Val Ile Val Pro Ala Glu Thr ProPhe

215 220 225

gag gat atc aag cag tac aac cca tca ggc gtg ttc atc tcc aac ggc 835

Glu Asp Ile Lys Gln Tyr Asn Pro Ser Gly Val Phe Ile Ser Asn Gly

230 235 240 245

cct ggc gat cct gca gca gca gac gtc atg gtt gat atc gtc cgc gaa 883

Pro Gly Asp Pro Ala Ala Ala Asp Val Met Val Asp Ile Val Arg Glu

250 255 260

gtt ctt gaa gcc gac att cca ttc ttt ggc atc tgc ttc ggc aac cag 931

Val Leu Glu Ala Asp Ile Pro Phe Phe Gly Ile Cys Phe Gly Asn Gln

265 270 275

att ctt ggc cgc gca ttc ggc atg gag acc tac aag ctg aag ttc ggc 979

Ile Leu Gly Arg Ala Phe Gly Met Glu Thr Tyr Lys Leu Lys Phe Gly

280 285 290

cac cgc ggc atc aac gtt cca gtg aag aac cac atc acc ggc aag atc 1027

His Arg Gly Ile Asn Val Pro Val Lys Asn His Ile Thr Gly Lys Ile

295 300 305

gac atc acc gcc cag aac cac ggc ttc gca ctc aag ggt gaa gca ggc 1075

Asp Ile Thr Ala Gln Asn His Gly Phe Ala Leu Lys Gly Glu Ala Gly

310 315 320 325

cag gaa ttc gag acc gat ttc ggc act gca att gtc acc cac acc tgc 1123

Gln Glu Phe Glu Thr Asp Phe Gly Thr Ala Ile Val Thr His Thr Cys

330 335 340

ctc aac gac ggc gtc gtt gaa ggt att gcg ctg aag tcc gga cgc gca 1171

Leu Asn Asp Gly Val Val Glu Gly Ile Ala Leu Lys Ser Gly Arg Ala

345 350 355

tac tcc gtt cag tac cac cca gag gcc gct gcc ggc cca aat gat gca 1219

Tyr Ser Val Gln Tyr His Pro Glu Ala Ala Ala Gly Pro Asn Asp Ala

360 365 370

agc ccc ctg ttt gac cag ttt gtt gag ctg atg gat gca gac gct cag 1267

Ser Pro Leu Phe Asp Gln Phe Val Glu Leu Met Asp Ala Asp Ala Gln

375 380 385

aag aaa ggc gca taaataacat gccaaagcgt tcagatatta 1309

Lys Lys Gly Ala

390

<210>4

<211>393

<212>PRT

<213>谷氨酸棒状杆菌

<400>4

Val Ser Lys Asp Thr Thr Thr Tyr Gln Gly Val Thr Glu Ile Gly Ser

1 5 10 15

Val Pro Ala Tyr Leu Val Leu Ala Asp Gly Arg Thr Phe Thr Gly Phe

20 25 30

Gly Phe Gly Ala Ile Gly Thr Thr Leu Gly Glu Ala Val Phe Thr Thr

35 40 45

Ala Met Thr Gly Tyr Gln Glu Thr Met Thr Asp Pro Ser Tyr His Arg

50 55 60

Gln Ile Val Val Ala Thr Ala Pro Gln Ile Gly Asn Thr Gly Trp Asn

65 70 75 80

Asp Glu Asp Asn Glu Ser Arg Asp Gly Lys Ile Trp Val Ala Gly Leu

85 90 95

Val Ile Arg Asp Leu Ala Ala Arg Val Ser Asn Trp Arg Ala Thr Thr

100 105 110

Ser Leu Gln Gln Glu Met Ala Gly Gln Gly Ile Val Gly Ile Gly Gly

115 120 125

Ile Asp Thr Arg Ala Leu Val Arg His Leu Arg Asn Glu Gly Ser Ile

130 135 140

Ala Ala Gly Ile Phe Ser Gly Ala Asp Ala Gln Arg Pro Val Glu Glu

145 150 155 160

Leu Val Glu Ile Val Lys Asn Gln Pro Ala Met Thr Gly Ala Asn Leu

165 170 175

Ser Val Glu Val Ser Ala Asp Glu Thr Tyr Val Ile Glu Ala Glu Gly

180 185 190

Glu Glu Arg His Thr Val Val Ala Tyr Asp Leu Gly Ile Lys Gln Asn

195 200 205

Thr Pro Arg Arg Phe Ser Ala Arg Gly Val Arg Thr Val Ile Val Pro

210 215 220

Ala Glu Thr Pro Phe Glu Asp Ile Lys Gln Tyr Asn Pro Ser Gly Val

225 230 235 240

Phe Ile Ser Asn Gly Pro Gly Asp Pro Ala Ala Ala Asp Val Met Val

245 250 255

Asp Ile Val Arg Glu Val Leu Glu Ala Asp Ile Pro Phe Phe Gly Ile

260 265 270

Cys Phe Gly Asn Gln Ile Leu Gly Arg Ala Phe Gly Met Glu Thr Tyr

275 280 285

Lys Leu Lys Phe Gly His Arg Gly Ile Asn Val Pro Val Lys Asn His

290 295 300

Ile Thr Gly Lys Ile Asp Ile Thr Ala Gln Asn His Gly Phe Ala Leu

305 310 315 320

Lys Gly Glu Ala Gly Gln Glu Phe Glu Thr Asp Phe Gly Thr Ala Ile

325 330 335

Val Thr His Thr Cys Leu Asn Asp Gly Val Val Glu Gly Ile Ala Leu

340 345 350

Lys Ser Gly Arg Ala Tyr Ser Val Gln Tyr His Pro Glu Ala Ala Ala

355 360 365

Gly Pro Asn Asp Ala Ser Pro Leu Phe Asp Gln Phe Val Glu Leu Met

370 375 380

Asp Ala Asp Ala Gln Lys Lys Gly Ala

385 390

<210>5

<211>1426

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1396)

<223>RXA00152

<400>5

gtcattgata tccaaggcac gaccgcgatt gtatggaaag aagcctaaat ttttaacaat 60

caaatagtac tggccattcc caactaaaac tggagtaacg atg aca gga cta atc 115

Met Thr Gly Leu Ile

1 5

ctc gcc ata gtt ttc ctg gtc ttt gtc gcc gtc gtg gtg atc aag tcc 163

Leu Ala Ile Val Phe Leu Val Phe Val Ala Val Val Val Ile Lys Ser

10 15 20

ata gcc ctg att ccc cag ggt gaa gcc gcc gtc att gaa cgc ctt ggt 211

Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val Ile Glu Arg Leu Gly

25 30 35

agc tac acc cgc acc gtt tca ggt ggc ctg acc ctg ctg gtt cca ttc 259

Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr Leu Leu Val Pro Phe

40 45 50

gtg gac cga gta cgc gca agg atc gac acc cgt gag cgc gtg gtc tca 307

Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg Glu Arg Val Val Ser

55 60 65

ttc cca ccg cag gct gtt att acc caa gac aac ctg acc gtg gcc atc 355

Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn Leu Thr Val Ala Ile

70 75 80 85

gat atc gtg gtg acc ttc caa atc aac gaa cca gag cgc gcc atc tac 403

Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro Glu Arg Ala Ile Tyr

90 95 100

ggc gtg gac aac tac atc gtc ggt gtg gag cag att tct gta gca aca 451

Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln Ile Ser Val Ala Thr

105 110 115

ctt cga gac gtt gtc ggt ggc atg acc ctg gaa gaa acc ctc act tca 499

Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu Glu Thr Leu Thr Ser

120 125 130

cgt gac gtg atc aac cgc cgc ctc cgt ggc gag ctc gat gca gca acc 547

Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu Leu Asp Ala Ala Thr

135 140 145

acc aaa tgg ggc ctg cgc atc agc cgt gtg gaa cta aag gca att gat 595

Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu Leu Lys Ala Ile Asp

150 155 160 165

ccg cca cca tcc atc cag caa tcg atg gaa aag cag atg aag gca gac 643

Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys Gln Met Lys Ala Asp

170 175 180

cgt gaa aag cgc gcc acc att ttg acc gca gaa ggt cag cgc gaa gcc 691

Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu Gly Gln Arg Glu Ala

185 190 195

gac atc aaa act gcc gaa ggt gaa aag caa gcc aag atc ctc caa gct 739

Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala Lys Ile Leu Gln Ala

200 205 210

gag ggt gaa aag cac gca tcc atc ctg aac gca gaa gca gaa cgc caa 787

Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala Glu Ala Glu Arg Gln

215 220 225

gcg atg atc ctg cgc gcc gaa ggt gaa cgc gca gca cgc tac ctc cag 835

Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala Ala Arg Tyr Leu Gln

230 235 240 245

gcg cag ggt gaa gcc cga gca atc caa aag gtc aac gca gca atc aag 883

Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val Asn Ala Ala Ile Lys

250 255 260

tct gcc aag ttg acc cca gag gtt ctt gct tat caa tac ctc gaa aag 931

Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr Gln Tyr Leu Glu Lys

265 270 275

ctt cct aag atc gca gag ggc aac gcc tcc aag atg tgg gtc atc cca 979

Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys Met Trp Val Ile Pro

280 285 290

agc cag ttc tcc gat tct ctg gaa ggt ttt gcg aag cag ttc ggc gca 1027

Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala Lys Gln Phe Gly Ala

295 300 305

aag gat gca gaa ggt gtc ttc cgc tac gaa cca aac acc gtg gat gaa 1075

Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro Asn Thr Val Asp Glu

310 315 320 325

gaa acc cgc gac atc gca aac gcc gac aac gtg gaa gac tgg ttc tcc 1123

Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val Glu Asp Trp Phe Ser

330 335 340

acc gaa tca gac cct gaa atc gca gca gca gtc gcc gca gca aac gcc 1171

Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val Ala Ala Ala Asn Ala

345 350 355

gtg gcc aac aag cca gtc gat cca gaa ccc ggt gag atc ctt tcc aag 1219

Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly Glu Ile Leu Ser Lys

360 365 370

aag acc gca cga cgc gtt gaa cct gaa gca gta ttg gag gct ttg caa 1267

Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val Leu Glu Ala Leu Gln

375 380 385

aac gga acc act aca caa cct gag gtt gag gca gca cct cct acc gca 1315

Ash Gly Thr Thr Thr Gln Pro Glu Val Glu Ala Ala Pro Pro Thr Ala

390 395 400 405

aac ttc gcc caa gaa ttc cct gca cca cag gca aac cct gaa gat tac 1363

Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala Asn Pro Glu Asp Tyr

410 415 420

tcc gac caa cac cga gag aat cct tac gga aac taatcaggca taagaaaagg 1416

Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn

425 430

cgggaaacac 1426

<210>6

<211>432

<212>PRT

<213>谷氨酸棒状杆菌

<400>6

Met Thr Gly Leu Ile Leu Ala Ile Val Phe Leu Val Phe Val Ala Val

1 5 10 15

Val Val Ile Lys Ser Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val

20 25 30

Ile Glu Arg Leu Gly Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr

35 40 45

Leu Leu Val Pro Phe Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg

50 55 60

Glu Arg Val Val Ser Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn

65 70 75 80

Leu Thr Val Ala Ile Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro

85 90 95

Glu Arg Ala Ile Tyr Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln

100 105 110

Ile Ser Val Ala Thr Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu

115 120 125

Glu Thr Leu Thr Ser Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu

130 135 140

Leu Asp Ala Ala Thr Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu

145 150 155 160

Leu Lys Ala Ile Asp Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys

165 170 175

Gln Met Lys Ala Asp Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu

180 185 190

Gly Gln Arg Glu Ala Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala

195 200 205

Lys Ile Leu Gln Ala Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala

210 215 220

Glu Ala Glu Arg Gln Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala

225 230 235 240

Ala Arg Tyr Leu Gln Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val

245 250 255

Asn Ala Ala Ile Lys Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr

260 265 270

Gln Tyr Leu Glu Lys Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys

275 280 285

Met Trp Val Ile Pro Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala

290 295 300

Lys Gln Phe Gly Ala Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro

305 310 315 320

Asn Thr Val Asp Glu Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val

325 330 335

Glu Asp Trp Phe Ser Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val

340 345 350

Ala Ala Ala Asn Ala Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly

355 360 365

Glu Ile Leu Ser Lys Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val

370 375 380

Leu Glu Ala Leu Gln Asn Gly Thr Thr Thr Gln Pro Glu Val Glu Ala

385 390 395 400

Ala Pro Pro Thr Ala Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala

405 410 415

Asn Pro Glu Asp Tyr Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn

420 425 430

<210>7

<211>1048

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1018)

<223>RXA00166

<400>7

tgcgacaacg ccactttgag agtccataat ttggcatcca acgccggcat cttggacggt 60

cgcgcgccgg gcccggggag gtttttatac ctatctaaag gtg tgg att tcg ttt 115

Val Trp Ile Ser Phe

1 5

gtt gca caa gga gat act gta tct cat gag cac gtt aag cga tat atc 163

Val Ala Gln Gly Asp Thr Val Ser His Glu His Val Lys Arg Tyr Ile

10 15 20

gct agt gaa ggt ggt ggt agc ccg cta gga tta aca tct gat atg aat 211

Ala Ser Glu Gly Gly Gly Ser Pro Leu Gly Leu Thr Ser Asp Met Asn

25 30 35

gat cca gct cat ccc cca att cca cca gta tca aaa cga gac gct ccg 259

Asp Pro Ala His Pro Pro Ile Pro Pro Val Ser Lys Arg Asp Ala Pro

40 45 50

gcg ttt agc gat ctt caa cat cga gca acc agc gcc agc gct ttt acc 307

Ala Phe Ser Asp Leu Gln His Arg Ala Thr Ser Ala Ser Ala Phe Thr

55 60 65

caa ggc agc acg act tat cac gat gtc cga cct gga tat ccg gct gag 355

Gln Gly Ser Thr Thr Tyr His Asp Val Arg Pro Gly Tyr Pro Ala Glu

70 75 80 85

gcc gtg gag tta gcc cgt ggg ttt ggc cga gtc ctg gat gtc ggt gca 403

Ala Val Glu Leu Ala Arg Gly Phe Gly Arg Val Leu Asp Val Gly Ala

90 95 100

ggt acc gga aaa cta acc agt gag cta aca gct gat cag gtc cta gcc 451

Gly Thr Gly Lys Leu Thr Ser Glu Leu Thr Ala Asp Gln Val Leu Ala

105 110 115

ctt gat cca agc atg gac atg ttg cgg gtg ttt cgc tcc gcg ctt ccg 499

Leu Asp Pro Ser Met Asp Met Leu Arg Val Phe Arg Ser Ala Leu Pro

120 125 130

gcg gtt ccc tgc tgg caa gcg aca gca gaa cac aca gga ata cgt gac 547

Ala Val Pro Cys Trp Gln Ala Thr Ala Glu His Thr Gly Ile Arg Asp

135 140 145

aac gcg gtt gat ctg att acg tgc gca caa acg tgg cat tgg gtt gac 595

Asn Ala Val Asp Leu Ile Thr Cys Ala Gln Thr Trp His Trp Val Asp

150 155 160 165

gtg acg gct gcc tca gcg gaa ttt gat cgg gtg att gca cct gag ggt 643

Val Thr Ala Ala Ser Ala Glu Phe Asp Arg Val Ile Ala Pro Glu Gly

170 175 180

gca gtc ctg ctc gtg tgg aat aac ctg gac acc tcc atc gcg tgg gta 691

Ala Val Leu Leu Val Trp Asn Asn Leu Asp Thr Ser Ile Ala Trp Val

185 190 195

cac cga ctc agt cgc att atg cat gcc ggc gat gta ctc aag ccg gga 739

His Arg Leu Ser Arg Ile Met His Ala Gly Asp Val Leu Lys Pro Gly

200 205 210

ttc acc cca gaa acc gca gct ccc tgg ata att gat cga gaa att cgc 787

Phe Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile Asp Arg Glu Ile Arg

215 220 225

acc acg tgg aat cag cac ctc acc cct gaa gaa atc atc cag ctc gct 835

Thr Thr Trp Asn Gln His Leu Thr Pro Glu Glu Ile Ile Gln Leu Ala

230 235 240 245

cac acg agg tcc tac tgg tta aac gcg tca gag aaa atc aaa gag cgt 883

His Thr Arg Ser Tyr Trp Leu Asn Ala Ser Glu Lys Ile Lys Glu Arg

250 255 260

gtt gat cag aac ctt cag tgg tat ctc tac gag cat ttg ggt ttc agt 931

Val Asp Gln Asn Leu Gln Trp Tyr Leu Tyr Glu His Leu Gly Phe Ser

265 270 275

ccc gac aat cca gtg gaa ctt ccc tat cgc tgt gat gca ttt tta ctt 979

Pro Asp Asn Pro Val Glu Leu Pro Tyr Arg Cys Asp Ala Phe Leu Leu

280 285 290

tca cgt tcc ggt acc ctg gca ggc aga tct tcc aat ctt taggagccct 1028

Ser Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser Asn Leu

295 300 305

cgccatgtac ctgttgaatc 1048

<210>8

<211>306

<212>PRT

<213>谷氨酸棒状杆菌

<400>8

Val Trp Ile Ser Phe Val Ala Gln Gly Asp Thr Val Ser His Glu His

1 5 10 15

Val Lys Arg Tyr Ile Ala Ser Glu Gly Gly Gly Ser Pro Leu Gly Leu

20 25 30

Thr Ser Asp Met Asn Asp Pro Ala His Pro Pro Ile Pro Pro Val Ser

35 40 45

Lys Arg Asp Ala Pro Ala Phe Ser Asp Leu Gln His Arg Ala Thr Ser

50 55 60

Ala Ser Ala Phe Thr Gln Gly Ser Thr Thr Tyr His Asp Val Arg Pro

65 70 75 80

Gly Tyr Pro Ala Glu Ala Val Glu Leu Ala Arg Gly Phe Gly Arg Val

85 90 95

Leu Asp Val Gly Ala Gly Thr Gly Lys Leu Thr Ser Glu Leu Thr Ala

100 105 110

Asp Gln Val Leu Ala Leu Asp Pro Ser Met Asp Met Leu Arg Val Phe

115 120 125

Arg Ser Ala Leu Pro Ala Val Pro Cys Trp Gln Ala Thr Ala Glu His

130 135 140

Thr Gly Ile Arg Asp Asn Ala Val Asp Leu Ile Thr Cys Ala Gln Thr

145 150 155 160

Trp His Trp Val Asp Val Thr Ala Ala Ser Ala Glu Phe Asp Arg Val

165 170 175

Ile Ala Pro Glu Gly Ala Val Leu Leu Val Trp Asn Asn Leu Asp Thr

180 185 190

Ser Ile Ala Trp Val His Arg Leu Ser Arg Ile Met His Ala Gly Asp

195 200 205

Val Leu Lys Pro Gly Phe Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile

210 215 220

Asp Arg Glu Ile Arg Thr Thr Trp Asn Gln His Leu Thr Pro Glu Glu

225 230 235 240

Ile Ile Gln Leu Ala His Thr Arg Ser Tyr Trp Leu Asn Ala Ser Glu

245 250 255

Lys Ile Lys Glu Arg Val Asp Gln Asn Leu Gln Trp Tyr Leu Tyr Glu

260 265 270

His Leu Gly Phe Ser Pro Asp Asn Pro Val Glu Leu Pro Tyr Arg Cys

275 280 285

Asp Ala Phe Leu Leu Ser Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser

290 295 300

Asn Leu

305

<210>9

<211>1162

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1132)

<223>RXA00278

<400>9

ggccacggag ttggtgtccg tcatggtgat gattctgtct gtcgtttctg tgttggcttt 60

gtgggtcggc atccgccccc gtttgcagga gtacttataa atg cac gct ttt cga 115

Met His Ala Phe Arg

1 5

cgc ccc cct cca ctc acc acg cga gtc ggc gct gca ttg ctg gcc gca 163

Arg Pro Pro Pro Leu Thr Thr Arg Val Gly Ala Ala Leu Leu Ala Ala

10 15 20

acg ctg ctt gct tcc tgc act cca aca cct gtg gaa ccg gca gaa acc 211

Thr Leu Leu Ala Ser Cys Thr Pro Thr Pro Val Glu Pro Ala Glu Thr

25 30 35

ttg act gct ttg gat ccc gat gcc ggt cca cca ctg cca ccg gat tct 259

Leu Thr Ala Leu Asp Pro Asp Ala Gly Pro Pro Leu Pro Pro Asp Ser

40 45 50

tcg att gaa gct ccc ggt gaa aaa gag ccc att gtg gaa gta ata gag 307

Ser Ile Glu Ala Pro Gly Glu Lys Glu Pro Ile Val Glu Val Ile Glu

55 60 65

aat tgg cca ggt tct tta cgc ccg gat gat ctg acc cct gag gag cgg 355

Asn Trp Pro Gly Ser Leu Arg Pro Asp Asp Leu Thr Pro Glu Glu Arg

70 75 80 85

gta cct ggc atc gtc aac cgg ggt cgc atc att gtg ggt gtg gat caa 403

Val Pro Gly Ile Val Asn Arg Gly Arg Ile Ile Val Gly Val Asp Gln

90 95 100

tcg caa aac ttg ctc agt ttc cgt gat ccg gtg act ggt gag ctg cgc 451

Ser Gln Asn Leu Leu Ser Phe Arg Asp Pro Val Thr Gly Glu Leu Arg

105 110 115

ggt ttt gaa gtg gaa tta gcg agg gaa att tcc cgc gac att ttc ggt 499

Gly Phe Glu Val Glu Leu Ala Arg Glu Ile Ser Arg Asp Ile Phe Gly

120 125 130

gac ccc aat aag gtg gat ttc cga ttc gtc ggc tcg tcc gac cgt ctg 547

Asp Pro Asn Lys Val Asp Phe Arg Phe Val Gly Ser Ser Asp Arg Leu

135 140 145

cgt tcc ctt gac caa ggt gat gta gat att gtg att cgt tcc gtc acg 595

Arg Ser Leu Asp Gln Gly Asp Val Asp Ile Val Ile Arg Ser Val Thr

150 155 160 165

atc acc gac gaa cgc gcc aaa ttg gtg gaa ttt tcc aca ccg tac ctg 643

Ile Thr Asp Glu Arg Ala Lys Leu Val Glu Phe Ser Thr Pro Tyr Leu

170 175 180

cgc acc caa acc cgc atg ttg acc atg gaa tct tca gga atc acg tcc 691

Arg Thr Gln Thr Arg Met Leu Thr Met Glu Ser Ser Gly Ile Thr Ser

185 190 195

atc gca gat cta ccc ggc cac acc att tgt gtc acc gat ggc tcc act 739

Ile Ala Asp Leu Pro Gly His Thr Ile Cys Val Thr Asp Gly Ser Thr

200 205 210

tca ttg cag cga gcc cgc acc att gcg ccg gag gcc tca atc tta aaa 787

Ser Leu Gln Arg Ala Arg Thr IleAla Pro Glu Ala Ser Ile Leu Lys

215 220 225

act cgc aat tgg tcc gat tgt ctc atg gcg ttg cag cag cat cag gct 835

Thr Arg Asn Trp Ser Asp Cys Leu Met Ala Leu Gln Gln His Gln Ala

230 235 240 245

cag gtc att ttg ggc gat gat gtc att ttg tcc ggc atc gca gca cag 883

Gln Val Ile Leu Gly Asp Asp Val Ile Leu Ser Gly Ile Ala Ala Gln

250 255 260

gat ccc tac acc gag att ctt gat acc tcc ctc gat tcc cat tcc tat 931

Asp Pro Tyr Thr Glu Ile Leu Asp Thr Ser Leu Asp Ser His Ser Tyr

265 270 275

gga gtg gca gcg gca tcg acc act gct gaa aca gac tct tcg ggg ttg 979

Gly Val Ala Ala Ala Ser Thr Thr Ala Glu Thr Asp Ser Ser Gly Leu

280 285 290

att cgg cag gta aac tac aca att gaa cgg atc cgc aca gac cgc atg 1027

Ile Arg Gln Val Asn Tyr Thr Ile Glu Arg Ile Arg Thr Asp Arg Met

295 300 305

tgg tgg aca atg ttc gac gat tgg ttc gga cct tat ctc tgg tcc tac 1075

Trp Trp Thr Met Phe Asp Asp Trp Phe Gly Pro Tyr Leu Trp Ser Tyr

310 315 320 325

ggt cca cca cag ctg cag tac atg cca gag gaa gaa ggg aca gaa aac 1123

Gly Pro Pro Gln Leu Gln Tyr Met Pro Glu Glu Glu Gly Thr Glu Asn

330 335 340

gat gaa gga taatgaagat ttcgatccag attcaccagc 1162

Asp Glu Gly

<210>10

<211>344

<212>PRT

<213>谷氨酸棒状杆菌

<400>10

Met His Ala Phe Arg Arg Pro Pro Pro Leu Thr Thr Arg Val Gly Ala

1 5 10 15

Ala Leu Leu Ala Ala Thr Leu Leu Ala Ser Cys Thr Pro Thr Pro Val

20 25 30

Glu Pro Ala Glu Thr Leu Thr Ala Leu Asp Pro Asp Ala Gly Pro Pro

35 40 45

Leu Pro Pro Asp Ser Ser Ile Glu Ala Pro Gly Glu Lys Glu Pro Ile

50 55 60

Val Glu Val Ile Glu Asn Trp Pro Gly Ser Leu Arg Pro Asp Asp Leu

65 70 75 80

Thr Pro Glu Glu Arg Val Pro Gly Ile Val Asn Arg Gly Arg Ile Ile

85 90 95

Val Gly Val Asp Gln Ser Gln Asn Leu Leu Ser Phe Arg Asp Pro Val

100 105 110

Thr Gly Glu Leu Arg Gly Phe Glu Val Glu Leu Ala Arg Glu Ile Ser

115 120 125

Arg Asp Ile Phe Gly Asp Pro Asn Lys Val Asp Phe Arg Phe Val Gly

130 135 140

Ser Ser Asp Arg Leu Arg Ser Leu Asp Gln Gly Asp Val Asp Ile Val

145 150 155 160

Ile Arg Ser Val Thr Ile Thr Asp Glu Arg Ala Lys Leu Val Glu Phe

165 170 175

Ser Thr Pro Tyr Leu Arg Thr Gln Thr Arg Met Leu Thr Met Glu Ser

180 185 190

Ser Gly Ile Thr Ser Ile Ala Asp Leu Pro Gly His Thr Ile Cys Val

195 200 205

Thr Asp Gly Ser Thr Ser Leu Gln Arg Ala Arg Thr Ile Ala Pro Glu

210 215 220

Ala Ser Ile Leu Lys Thr Arg Asn Trp Ser Asp Cys Leu Met Ala Leu

225 230 235 240

Gln Gln His Gln Ala Gln Val Ile Leu Gly Asp Asp Val Ile Leu Ser

245 250 255

Gly Ile Ala Ala Gln Asp Pro Tyr Thr Glu Ile Leu Asp Thr Ser Leu

260 265 270

Asp Ser His Ser Tyr Gly Val Ala Ala Ala Ser Thr Thr Ala Glu Thr

275 280 285

Asp Ser Ser Gly Leu Ile Arg Gln Val Asn Tyr Thr Ile Glu Arg Ile

290 295 300

Arg Thr Asp Arg Met Trp Trp Thr Met Phe Asp Asp Trp Phe Gly Pro

305 310 315 320

Tyr Leu Trp Ser Tyr Gly Pro Pro Gln Leu Gln Tyr Met Pro Glu Glu

325 330 335

Glu Gly Thr Glu Asn Asp Glu Gly

340

<210>11

<211>1558

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1528)

<223>RXA00323

<400>11

ttccttattc gtaggagtgt tctaatttcg gtgcggttct cagtgaacca cccaagctgg 60

acacctccca cccccgtgtc atcaaaaaac cgcgacatcc ttg agt aac tct gag 115

Leu Ser Asn Ser Glu

1 5

aaa aac tac ccc cga tgg gag tat aaa agt ggc aaa tgc gca gtc gat 163

Lys Asn Tyr Pro Arg Trp Glu Tyr Lys Ser Gly Lys Cys Ala Val Asp

10 15 20

gtc cca tcg ctg cgt aga tta gtt ttc atg aac agc gaa cag gaa ttt 211

Val Pro Ser Leu Arg Arg Leu Val Phe Met Asn Ser Glu Gln Glu Phe

25 30 35

gta ctc agc gcc att gaa gaa cgc gac att aag ttt gtg cgt cta tgg 259

Val Leu Ser Ala Ile Glu Glu Arg Asp Ile Lys Phe Val Arg Leu Trp

40 45 50

ttc act gac att ctt ggc cac ttg aag tca gtg gtt gtg gct cct gca 307

Phe Thr Asp Ile Leu Gly His Leu Lys Ser Val Val Val Ala Pro Ala

55 60 65

gaa cta gag tct gcg ttg gaa gaa ggc atc gga ttc gat ggc tca gcc 355

Glu Leu Glu Ser Ala Leu Glu Glu Gly Ile Gly Phe Asp Gly Ser Ala

70 75 80 85

att gag ggc tac gcg cgt atc tcg gaa gcg gac acc att gcc cgc cca 403

Ile Glu Gly Tyr Ala Arg Ile Ser Glu Ala Asp Thr Ile Ala Arg Pro

90 95 100

gat cca tcg aca ttc cag gtc ctc cca cta gaa gcg ggc atc tca aaa 451

Asp Pro Ser Thr Phe Gln Val Leu Pro Leu Glu Ala Gly Ile Ser Lys

105 110 115

ctg cag gca gca cgc ctg ttt tgc gat gtc acg atg cca gac gga cag 499

Leu Gln Ala Ala Arg Leu Phe Cys Asp Val Thr Met Pro Asp Gly Gln

120 125 130

cca tct ttt tct gac ccg cgc caa gtg ctg cgc agg cag gtc caa cta 547

Pro Ser Phe Ser Asp Pro Arg Gln Val Leu Arg Arg Gln Val Gln Leu

135 140 145

gct gca gat gaa ggc ttg acc tgc atg atc tca cca gag att gag ttc 595

Ala Ala Asp Glu Gly Leu Thr Cys Met Ile Ser Pro Glu Ile Glu Phe

150 155 160 165

tat ttg gtg caa agc ctt cgc acc aac gga ctg cca cct gtg ccc act 643

Tyr Leu Val Gln Ser Leu Arg Thr Asn Gly Leu Pro Pro Val Pro Thr

170 175 180

gac aac ggc gga tat ttc gac caa gcc aca ttc aat gag gcg ccg aat 691

Asp Asn Gly Gly Tyr Phe Asp Gln Ala Thr Phe Asn Glu Ala Pro Asn

185 190 195

ttc cgt cga aac gcg atg gta gcg ctg gag gaa ctc ggc atc cct gtc 739

Phe Arg Arg Asn Ala Met Val Ala Leu Glu Glu Leu Gly Ile Pro Val

200 205 210

gag ttc tcc cac cat gaa act gca cct ggc cag caa gaa atc gat tta 787

Glu Phe Ser His His Glu Thr Ala Pro Gly Gln Gln Glu Ile Asp Leu

215 220 225

cgc cat gcg gat gcg ctc acc atg gcc gac aac atc atg acc ttc cgc 835

Arg His Ala Asp Ala Leu Thr Met Ala Asp Asn Ile Met Thr Phe Arg

230 235 240 245

tac atc atg aaa cag gtg gca agg gac caa ggc gtc ggg gca tca ttt 883

Tyr Ile Met Lys Gln Val Ala Arg Asp Gln Gly Val Gly Ala Ser Phe

250 255 260

atg ccc aag cca ttc caa gaa cat gca ggc tcc gcc atg cac acg cac 931

Met Pro Lys Pro Phe Gln Glu His Ala Gly Ser Ala Met His Thr His

265 270 275

atg tcc tta ttt gag ggc gat acc aac gcg ttc cac gat cca gac gat 979

Met Ser Leu Phe Glu Gly Asp Thr Asn Ala Phe His Asp Pro Asp Asp

280 285 290

tct tac atg ctg tcc aaa acc gca aaa cag ttc atc gct gga atc ttg 1027

Ser Tyr Met Leu Ser Lys Thr Ala Lys Gln Phe Ile Ala Gly Ile Leu

295 300 305

cat cac gct cca gaa ttc acc gct gtg acc aac cag tgg gtc aat tcc 1075

His His Ala Pro Glu Phe Thr Ala Val Thr Asn Gln Trp Val Asn Ser

310 315 320 325

tac aaa cgc atc gtg tac gga aac gaa gct cca act gcg gca acc tgg 1123

Tyr Lys Arg Ile Val Tyr Gly Asn Glu Ala Pro Thr Ala Ala Thr Trp

330 335 340

ggt gta tct aat cgt tct gcg ctg gtt cgt gtt cct acc tac cgt ttg 1171

Gly Val Ser Asn Arg Sar Ala Leu Val Arg Val Pro Thr Tyr Arg Leu

345 350 355

aat aag gag gag tcg cgc cgg gtg gag gtg cgt ctt cct gat acc gct 1219

Asn Lys Glu Glu Ser Arg Arg Val Glu Val Arg Leu Pro Asp Thr Ala

360 365 370

tgt aac cca tat ttg gcg ttt tca gtg atg ctc ggc gct ggt ttg aaa 1267

Cys Asn Pro Tyr Leu Ala Phe Ser Val Met Leu Gly Ala Gly Leu Lys

375 380 385

ggc att aaa gaa ggt tat gag ctc gac gag cca gct gag gac gat atc 1315

Gly Ile Lys Glu Gly Tyr Glu Leu Asp Glu Pro Ala Glu Asp Asp Ile

390 395 400 405

tcc aac ttg agc ttc cgg gaa cgt cgc gcc atg ggc tac aac gat ctg 1363

Ser Asn Leu Ser Phe Arg Glu Arg Arg Ala Met Gly Tyr Asn Asp Leu

410 415 420

cca agc agc ctt gat cag gca ctg cgc caa atg gaa aag tca gag ctt 1411

Pro Ser Ser Leu Asp Gln Ala Leu Arg Gln Met Glu Lys Ser Glu Leu

425 430 435

gtt gct gac atc ctc ggt gag cac gtt ttt gag ttt ttc ttg cgc aat 1459

Val Ala Asp Ile Leu Gly Glu His Val Phe Glu Phe Phe Leu Arg Asn

440 445 450

aag tgg cgt gaa tgg cgt gac tac caa gag cag atc act ccg tgg gag 1507

Lys Trp Arg Glu Trp Arg Asp Tyr Gln Glu Gln Ile Thr Pro Trp Glu

455 460 465

ctc cga aac aat ctt gat tac tagacttttg cactccaatg gaaaccctac 1558

Leu Arg Asn Asn Leu Asp Tyr

470 475

<210>12

<211>476

<212>PRT

<213>谷氨酸棒状杆菌

<400>12

Leu Ser Asn Ser Glu Lys Asn Tyr Pro Arg Trp Glu Tyr Lys Ser Gly

1 5 10 15

Lys Cys Ala Val Asp Val Pro Ser Leu Arg Arg Leu Val Phe Met Asn

20 25 30

Ser Glu Gln Glu Phe Val Leu Ser Ala Ile Glu Glu Arg Asp Ile Lys

35 40 45

Phe Val Arg Leu Trp Phe Thr Asp Ile Leu Gly His Leu Lys Ser Val

50 55 60

Val Val Ala Pro Ala Glu Leu Glu Ser Ala Leu Glu Glu Gly Ile Gly

65 70 75 80

Phe Asp Gly Ser Ala Ile Glu Gly Tyr Ala Arg Ile Ser Glu Ala Asp

85 90 95

Thr Ile Ala Arg Pro Asp Pro Ser Thr Phe Gln Val Leu Pro Leu Glu

100 105 110

Ala Gly Ile Ser Lys Leu Gln Ala Ala Arg Leu Phe Cys Asp Val Thr

115 120 125

Met Pro Asp Gly Gln Pro Ser Phe Ser Asp Pro Arg Gln Val Leu Arg

130 135 140

Arg Gln Val Gln Leu Ala Ala Asp Glu Gly Leu Thr Cys Met Ile Ser

145 150 155 160

Pro Glu Ile Glu Phe Tyr Leu yal Gln Ser Leu Arg Thr Asn Gly Leu

165 170 175

Pro Pro Val Pro Thr Asp Asn Gly Gly Tyr Phe Asp Gln Ala Thr Phe

180 185 190

Asn Glu Ala Pro Asn Phe Arg Arg Asn Ala Met Val Ala Leu Glu Glu

195 200 205

Leu Gly Ile Pro Val Glu Phe Ser His His Glu Thr Ala Pro Gly Gln

210 215 220

Gln Glu Ile Asp Leu Arg His Ala Asp Ala Leu Thr Met Ala Asp Asn

225 230 235 2 40

Ile Met Thr Phe Arg Tyr Ile Met Lys Gln Val Ala Arg Asp Gln Gly

245 250 255

Val Gly Ala Ser Phe Met Pro Lys Pro Phe Gln Glu His Ala Gly Ser

260 265 270

Ala Met His Thr His Met Ser Leu Phe Glu Gly Asp Thr Asn Ala Phe

275 280 285

His Asp Pro Asp Asp Ser Tyr Met Leu Ser Lys Thr Ala Lys Gln Phe

290 295 300

Ile Ala Gly Ile Leu His His Ala Pro Glu Phe Thr Ala Val Thr Asn

305 310 315 320

Gln Trp Val Asn Ser Tyr Lys Arg Ile Val Tyr Gly Asn Glu Ala Pro

325 330 335

Thr Ala Ala Thr Trp Gly Val Ser Asn Arg Ser Ala Leu Val Arg Val

340 345 350

ProThr Tyr Arg Leu Asn Lys Glu Glu Ser Arg Arg Val Glu Val Arg

355 360 365

Leu Pro Asp Thr Ala Cys Asn Pro Tyr Leu Ala Phe Ser Val Met Leu

370 375 380

Gly Ala Gly Leu Lys Gly Ile Lys Glu Gly Tyr Glu Leu Asp Glu Pro

385 390 395 400

Ala Glu Asp Asp Ile Ser Asn Leu Ser Phe Arg Glu Arg Arg Ala Met

405 410 415

Gly Tyr Asn Asp Leu Pro Ser Ser Leu Asp Gln Ala Leu Arg Gln Met

420 425 430

Glu Lys Ser Glu Leu Val Ala Asp Ile Leu Gly Glu His Val Phe Glu

435 440 445

Phe Phe Leu Arg Asn Lys Trp Arg Glu Trp Arg Asp Tyr Gln Glu Gln

450 455 460

Ile Thr Pro Trp Glu Leu Arg Asn Asn Leu Asp Tyr

465 470 475

<210>13

<211>1615

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1585)

<223>RXA00330

<400>13

atctttaaag cacatcctaa aaccgacaat tgaaagtgat cagcaacact ttagggtatc 60

gcgtgggcga agtcaccttt ttcaacatat ttgagacggt gtg ggg gag tat tgt 115

Val Gly Glu Tyr Cys

1 5

gtc acc cct tgg gat agg gtt ata tcc gtg gac tac att tcg acg cgt 163

Val Thr Pro Trp Asp Arg Val Ile Ser Val Asp Tyr Ile Ser Thr Arg

10 15 20

gat gcc agc cgt acc cct gcc cgc ttc agt gat att ttg ctg ggc ggt 211

Asp Ala Ser Arg Thr Pro Ala Arg Phe Ser Asp Ile Leu Leu Gly Gly

25 30 35

cta gca cca gac ggc ggc ctg tac ctg cct gca acc tac cct caa cta 259

Leu Ala Pro Asp Gly Gly Leu Tyr Leu Pro Ala Thr Tyr Pro Gln Leu

40 45 50

gat gat gcc cag ctg agt aaa tgg cgt gag gta tta gcc aac gaa gga 307

Asp Asp Ala Gln Leu Ser Lys Trp Arg Glu Val Leu Ala Asn Glu Gly

55 60 65

tac gca gct ttg gct gct gaa gtt atc tcc ctg ttt gtt gat gac atc 355

Tyr Ala Ala Leu Ala Ala Glu Val Ile Ser Leu Phe Val Asp Asp Ile

70 75 80 85

cca gta gaa gac atc aag gcg atc acc gca cgc gcc tac acc tac ccg 403

Pro Val Glu Asp Ile Lys Ala Ile Thr Ala Arg Ala Tyr Thr Tyr Pro

90 95 100

aag ttc aac agc gaa gac atc gtt cct gtc acc gaa ctc gag gac aac 451

Lys Phe Asn Ser Glu Asp Ile Val Pro Val Thr Glu Leu Glu Asp Asn

105 110 115

att tac ctg ggc cac ctt tcc gaa ggc cca acc gct gca ttc aaa gac 499

Ile Tyr Leu Gly His Leu Ser Glu Gly Pro Thr Ala Ala Phe Lys Asp

120 125 130

atg gcc atg cag ctg ctc ggc gaa ctt ttc gaa tac gag ctt cgc cgc 547

Met Ala Met Gln Leu Leu Gly Glu Leu Phe Glu Tyr Glu Leu Arg Arg

135 140 145

cgc aac gaa acc atc aac atc ctg ggc gct acc tct ggc gat acc ggc 595

Arg Asn Glu Thr Ile Asn Ile Leu Gly Ala Thr Ser Gly Asp Thr Gly

150 155 160 165

tcc tct gcg gaa tac gcc atg cgc ggc cgc gag gga atc cgc gta ttc 643

Ser Ser Ala Glu Tyr Ala Met Arg Gly Arg Glu Gly Ile Arg Val Phe

170 175 180

atg ctg acc cca gct ggc cgc atg acc cca ttc cag caa gca cag atg 691

Met Leu Thr Pro Ala Gly Arg Met Thr Pro Phe Gln Gln Ala Gln Met

185 190 195

ttt ggc ctt gac gat cca aac atc ttc aac atc gcc ctc gac ggc gtt 739

Phe Gly Leu Asp Asp Pro Asn Ile Phe Asn Ile Ala Leu Asp Gly Val

200 205 210

ttc gac gat tgc caa gac gta gtc aag gct gtc tcc gcc gac gca gaa 787

Phe Asp Asp Cys Gln Asp Val Val Lys Ala Val Ser Ala Asp Ala Glu

215 220 225

ttc aaa aaa gac aac cgc atc ggt gcc gtg aac tcc atc aac tgg gca 835

Phe Lys Lys Asp Asn Arg Ile Gly Ala Val Asn Ser Ile Asn Trp Ala

230 235 240 245

cgc ctt atg gca cag gtt gtg tac tac gtt tcc tca tgg atc cgc acc 883

Arg Leu Met Ala Gln Val Val Tyr Tyr Val Ser Ser Trp Ile Arg Thr

250 255 260

aca acc agc aat gac caa aag gtc agc ttc tcc gta cca acc ggc aac 931

Thr Thr Ser Asn Asp Gln Lys Val Ser Phe Ser Val Pro Thr Gly Asn

265 270 275

ttc ggt gac att tgc gca ggc cac atc gcc cgc caa atg gga ctt ccc 979

Phe Gly Asp Ile Cys Ala Gly His Ile Ala Arg Gln Met Gly Leu Pro

280 285 290

atc gat cgc ctc atc gtg gcc acc aac gaa aac gat gtg ctc gac gag 1027

Ile Asp Arg Leu Ile Val Ala Thr Asn Glu Asn Asp Val Leu Asp Glu

295 300 305

ttc ttc cgt acc ggc gac tac cga gtc cgc agc tcc gca gac acc cac 1075

Phe Phe Arg Thr Gly Asp Tyr Arg Val Arg Ser Ser Ala Asp Thr His

310 315 320 325

gag acc tcc tca cct tcg atg gat atc tcc cgc gcc tcc aac ttc gag 1123

Glu Thr Ser Ser Pro Ser Met Asp Ile Ser Arg Ala Ser Asn Phe Glu

330 335 340

cgt ttc atc ttc gac ctg ctc ggc cgc gac gcc acc cgc gtc aac gat 1171

Arg Phe Ile Phe Asp Leu Leu Gly Arg Asp Ala Thr Arg Val Asn Asp

345 350 355

cta ttt ggt acc cag gtt cgc caa ggc gga ttc tca ctg gct gat gac 1219

Leu Phe Gly Thr Gln Val Arg Gln Gly Gly Phe Ser Leu Ala Asp Asp

360 365 370

gcc aac ttt gag aag gct gca gca gaa tac ggt ttc gcc tcc gga cga 1267

Ala Asn Phe Glu Lys Ala Ala Ala Glu Tyr Gly Phe Ala Ser Gly Arg

375 380 385

tcc acc cat gct gac cgt gtg gca acc atc gct gac gtg cat tcc cgc 1315

Ser Thr His Ala Asp Arg Val Ala Thr Ile Ala Asp Val His Ser Arg

390 395 400 405

ctc gac gta cta atc gat ccc cac acc gcc gac ggc gtt cac gtg gca 1363

Leu Asp Val Leu Ile Asp Pro His Thr Ala Asp Gly Val His Val Ala

410 415 420

cgc cag tgg agg gac gag gtc aac acc cca atc atc gtc cta gaa act 1411

Arg Gln Trp Arg Asp Glu Val Asn Thr Pro Ile Ile Val Leu Glu Thr

425 430 435

gca ctc cca gtg aaa ttt gcc gac acc atc gtc gaa gca att ggt gaa 1459

Ala Leu Pro Val Lys Phe Ala Asp Thr Ile Val Glu Ala Ile Gly Glu

440 445 450

gca cct caa act cca gag cgt ttc gcc gcg atc atg gat gct cca ttc 1507

Ala Pro Gln Thr Pro Glu Arg Phe Ala Ala Ile Met Asp Ala Pro Phe

455 460 465

aag gtt tcc gac cta cca aac gac acc gat gca gtt aag cag tac ata 1555

Lys Val Ser Asp Leu Pro Asn Asp Thr Asp Ala Val Lys Gln Tyr Ile

470 475 480 485

gtc gat gcg att gca aac act tcc gtg aag taacttgctt tacgccaagg 1605

Val Asp Ala Ile Ala Asn Thr Ser Val Lys

490 495

cctgattcct 1615

<210>14

<211>495

<212>PRT

<213>谷氨酸棒状杆菌

<400>14

Val Gly Glu Tyr Cys Val Thr Pro Trp Asp Arg Val Ile Ser Val Asp

1 5 10 15

Tyr Ile Ser Thr Arg Asp Ala Ser Arg Thr Pro Ala Arg Phe Ser Asp

20 25 30

Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu Pro Ala

35 40 45

Thr Tyr Pro Gln Leu Asp Asp Ala Gln Leu Ser Lys Trp Arg Glu Val

50 55 60

Leu Ala Asn Glu Gly Tyr Ala Ala Leu Ala Ala Glu Val Ile Ser Leu

65 70 75 80

Phe Val Asp Asp Ile Pro Val Glu Asp Ile Lys Ala Ile Thr Ala Arg

85 90 95

Ala Tyr Thr Tyr Pro Lys Phe Asn Ser Glu Asp Ile Val Pro Val Thr

100 105 110

Glu Leu Glu Asp Asn Ile Tyr Leu Gly His Leu Ser Glu Gly Pro Thr

115 120 125

Ala Ala Phe Lys Asp Met Ala Met Gln Leu Leu Gly Glu Leu Phe Glu

130 135 140

Tyr Glu Leu Arg Arg Arg Asn Glu Thr Ile Asn Ile Leu Gly Ala Thr

145 150 155 160

Ser Gly Asp Thr Gly Ser Ser Ala Glu Tyr Ala Met Arg Gly Arg Glu

165 170 175

Gly Ile Arg Val Phe Met Leu Thr Pro Ala Gly Arg Met Thr Pro Phe

180 185 190

Gln Gln Ala Gln Met Phe Gly Leu Asp Asp Pro Asn Ile Phe Asn Ile

195 200 205

Ala Leu Asp Gly Val Phe Asp Asp Cys Gln Asp Val Val Lys Ala Val

210 215 220

Ser Ala Asp Ala Glu Phe Lys Lys Asp Asn Arg Ile Gly Ala Val Asn

225 230 235 240

Ser Ile Asn Trp Ala Arg Leu Met Ala Gln Val Val Tyr Tyr Val Ser

245 250 255

Ser Trp Ile Arg Thr Thr Thr Ser Asn Asp Gln Lys Val Ser Phe Ser

260 265 270

Val Pro Thr Gly Asn Phe Gly Asp Ile Cys Ala Gly His Ile Ala Arg

275 280 285

Gln Met Gly Leu Pro Ile Asp Arg Leu Ile Val Ala Thr Asn Glu Asn

290 295 300

Asp Val Leu Asp Glu Phe Phe Arg Thr Gly Asp Tyr Arg Val Arg Ser

305 310 315 320

Ser Ala Asp Thr His Glu Thr Ser Ser Pro Ser Met Asp Ile Ser Arg

325 330 335

Ala Ser Asn Phe Glu Arg Phe Ile Phe Asp Leu Leu Gly Arg Asp Ala

340 345 350

Thr Arg Val Asn Asp Leu Phe Gly Thr Gln Val Arg Gln Gly Gly Phe

355 360 365

Ser Leu Ala Asp Asp Ala Asn Phe Glu Lys Ala Ala Ala Glu Tyr Gly

370 375 380

Phe Ala Ser Gly Arg Ser Thr His Ala Asp Arg Val Ala Thr Ile Ala

385 390 395 400

Asp Val His Ser Arg Leu Asp Val Leu Ile Asp Pro His Thr Ala Asp

405 410 415

Gly Val His Val Ala Arg Gln Trp Arg Asp Glu Val Asn Thr Pro Ile

420 425 430

Ile Val Leu Glu Thr Ala Leu Pro Val Lys Phe Ala Asp Thr Ile Val

435 440 445

Glu Ala Ile Gly Glu Ala Pro Gln Thr Pro Glu Arg Phe Ala Ala Ile

450 455 460

Met Asp Ala Pro Phe Lys Val Ser Asp Leu Pro Asn Asp Thr Asp Ala

465 470 475 480

Val Lys Gln Tyr Ile Val Asp Ala Ile Ala Asn Thr Ser Val Lys

485 490 495

<210>15

<211>1474

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1444)

<223>RXA00383

<400>15

ccatatcttt aaccttggtc atggtgtgct tcctaatacg gtggcggaag atattactga 60

agccgtctcc atcattcatt cttaaactaa gaggagtttc atg cgt ttt gcc atc 115

Met Arg Phe Ala Ile

1 5

atc ggt gca ggc ctt gcg ggt ctg act gct gca tat gag atc cat aaa 163

Ile Gly Ala Gly Leu Ala Gly Leu Thr Ala Ala Tyr Glu Ile His Lys

10 15 20

gct gat ccc act gcc caa ate gat gtg ttg gaa gca ggc gaa cgc att 211

Ala Asp Pro Thr Ala Gln Ile Asp Val Leu Glu Ala Gly Glu Arg Ile

25 30 35

ggc ggc aag ctt ttt acg gtg ccg ttt gct tcc gga cct acc gat att 259

Gly Gly Lys Leu Phe Thr Val Pro Phe Ala Ser Gly Pro Thr Asp Ile

40 45 50

gga gcg gag gcg ttt tta gct gcg cgt tcc gat gcg gtg gag ttt ttt 307

Gly Ala Glu Ala Phe Leu Ala Ala Arg Ser Asp Ala Val Glu Phe Phe

55 60 65

act gag ctt ggg ttg gct gat tct ttg gtc agc ccg tct gct gcg aag 355

Thr Glu Leu Gly Leu Ala Asp Ser Leu Val Ser Pro Ser Ala Ala Lys

70 75 80 85

tct cag tat ttc gcg ggc ggt gcg ctg cat gcg ttc ccc gca ggt gga 403

Ser Gln Tyr Phe Ala Gly Gly Ala Leu His Ala Phe Pro Ala Gly Gly

90 95 100

gtg atg ggt att cca agc aat cca cca gca ggc gcg caa gac acc gct 451

Val Met Gly Ile Pro Ser Asn Pro Pro Ala Gly Ala Gln Asp Thr Ala

105 110 115

ttt gat tgg act cct ggc caa gac att tct gtt ggc gcc tta gtg cgc 499

Phe Asp Trp Thr Pro Gly Gln Asp Ile Ser Val Gly Ala Leu Val Arg

120 125 130

cgt cag tat ggc gat gag atc gtc gat act gtg gtg tct tcg ctg ctt 547

Arg Gln Tyr Gly Asp Glu Ile Val Asp Thr Val Val Ser Ser Leu Leu

135 140 145

ggt ggc gtt tat tcc tcc acc gct gat gat ctg ggt gtg cgc gct tcc 595

Gly Gly Val Tyr Ser Ser Thr Ala Asp Asp Leu Gly Val Arg Ala Ser

150 155 160 165

gtg ccg gca ctt gct gca gcc ctt gat cag ctg gct gag gcc ggc gag 643

Val Pro Ala Leu Ala Ala Ala Leu Asp Gln Leu Ala Glu Ala Gly Glu

170 175 180

ccg gtg act ctg tca gct gcg gtc aag gcc gtg gaa gct cag cgg gaa 69l

Pro Val Thr Leu Ser Ala Ala Val Lys Ala Val Glu Ala Gln Arg Glu

185 190 195

gcc gca aaa aca act tca gaa acc cgc ccc gtt ttc cag acc ttc aag 739

Ala Ala Lys Thr Thr Ser Glu Thr Arg Pro Val Phe Gln Thr Phe Lys

200 205 210

ggc gga tac gcg gag ctg tac gaa gcg ttg gca gag caa tgc ggt gca 787

Gly Gly Tyr Ala Glu Leu Tyr Glu Ala Leu Ala Glu Gln Cys Gly Ala

215 220 225

gat att cac ttg gat agt ttc gtt tcc gcc atc acc aaa gat ggt gaa 835

Asp Ile His Leu Asp Ser Phe Val Ser Ala Ile Thr Lys Asp Gly Glu

230 235 240 245

ggt ttt gcc atc aag ggc ggt ggc gaa ggc acc tac gac aag gtg att 883

Gly Phe Ala Ile Lys Gly Gly Gly Glu Gly Thr Tyr Asp Lys Val Ile

250 255 260

ttg gcg gtt ccc gct cca acc gcc gct gtg ctg ctc cgc gac ttg gca 931

Leu Ala Val Pro Ala Pro Thr Ala Ala Val Leu Leu Arg Asp Leu Ala

265 270 275

ccg gcc gca gcg cca cat ttg cgc gca att aag ttg gct tct tca gca 979

Pro Ala Ala Ala Pro His Leu Arg Ala Ile Lys Leu Ala Ser Ser Ala

280 285 290

gtc gtc ggc atg cgt ttc gat tcc agc gag ggc ctg ccc gac aac tcc 1027

Val Val Gly Met Arg Phe Asp Ser Ser Glu Gly Leu Pro Asp Asn Ser

295 300 305

ggc gtc ctg gtc gct gtt aat gag ccg ggc atc acg gcg aag gcc ttc 1075

Gly Val Leu Val Ala Val Asn Glu Pro Gly Ile Thr Ala Lys Ala Phe

310 315 320 325

acg ttc tcc tca aag aag tgg cct cac ctg gag gct cgc ggg ggc gcg 1123

Thr Phe Ser Ser Lys Lys Trp Pro His Leu Glu Ala Arg Gly Gly Ala

330 335 340

ctc gtg cgc gcg tcg ttc ggc agg cta ggc gat gag gcg tcg gca cgc 1171

Leu Val Arg Ala Ser Phe Gly Arg Leu Gly Asp Glu Ala Ser Ala Arg

345 350 355

atg gac gag gat ttg ctt gtc gac gcc gcc ctc gac gat ctc ctc acc 1219

Met Asp Glu Asp Leu Leu Val Asp Ala Ala Leu Asp Asp Leu Leu Thr

360 365 370

ata acc ggg ttc gac ggc cgg gct gcc gga ctg ggt gaa att ttc gtg 1267

Ile Thr Gly Phe Asp Gly Arg Ala Ala Gly Leu Gly Glu Ile Phe Val

375 380 385

cag cgc tgg ttc ggt ggg ctc cca gcc tat gga gtt gat cac att gct 1315

Gln Arg Trp Phe Gly Gly Leu Pro Ala Tyr Gly Val Asp Hi s Ile Ala

390 395 400 405

acc gtt tcg gct gcg cgt gca gag atc gca gcc gtg cct ggc gtg gaa 1363

Thr Val Ser Ala Ala Arg Ala Glu Ile Ala Ala Val Pro Gly Val Glu

410 415 420

gca att ggc gcg tgg gct ggg gga gtg gga gtt ccc gca gtt atc gca 1411

Ala Ile Gly Ala Trp Ala Gly Gly Val Gly Val Pro Ala Val Ile Ala

425 430 435

gat gcc cag gca gca gta cac agg ttg ctg gga taagcaccca aaaacactat 1464

Asp Ala Gln Ala Ala Val His Arg Leu Leu Gly

440 445

tgaccacccc 1474

<210>16

<211>448

<212>PRT

<213>谷氨酸棒状杆菌

<400>16

Met Arg Phe Ala Ile Ile Gly Ala Gly Leu Ala Gly Leu Thr Ala Ala

1 5 10 15

Tyr Glu Ile His Lys Ala Asp Pro Thr Ala Gln Ile Asp Val Leu Glu

20 25 30

Ala Gly Glu Arg Ile Gly Gly Lys Leu Phe Thr Val Pro Phe Ala Ser

35 40 45

Gly Pro Thr Asp Ile Gly Ala Glu Ala Phe Leu Ala Ala Arg Ser Asp

50 55 60

Ala Val Glu Phe Phe Thr Glu Leu Gly Leu Ala Asp Ser Leu Val Ser

65 70 75 80

Pro Ser Ala Ala Lys Ser Gln Tyr Phe Ala Gly Gly Ala Leu His Ala

85 90 95

Phe Pro Ala Gly Gly Val Met Gly Ile Pro Ser Asn Pro Pro Ala Gly

100 105 110

Ala Gln Asp Thr Ala Phe Asp Trp Thr Pro Gly Gln Asp Ile Ser Val

115 120 125

Gly Ala Leu Val Arg Arg Gln Tyr Gly Asp Glu Ile Val Asp Thr Val

130 135 140

Val Ser Ser Leu Leu Gly Gly Val Tyr Ser Ser Thr Ala Asp Asp Leu

145 150 155 160

Gly Val Arg Ala Ser Val Pro Ala Leu Ala Ala Ala Leu Asp Gln Leu

165 170 175

Ala Glu Ala Gly Glu Pro Val Thr Leu Ser Ala Ala Val Lys Ala Val

180 185 190

Glu Ala Gln Arg Glu Ala Ala Lys Thr Thr Ser Glu Thr Arg Pro Val

195 200 205

Phe Gln Thr Phe Lys Gly Gly Tyr Ala Glu Leu Tyr Glu Ala Leu Ala

210 215 220

Glu Gln Cys Gly Ala Asp Ile His Leu Asp Ser Phe Val Ser Ala Ile

225 230 235 240

Thr Lys Asp Gly Glu Gly Phe Ala Iie Lys Gly Gly Gly Glu Gly Thr

245 250 255

Tyr Asp Lys Val Ile Leu Ala Val Pro Ala Pro Thr Ala Ala Val Leu

260 265 270

Leu Arg Asp Leu Ala Pro Ala Ala Ala Pro His Leu Arg Ala Ile Lys

275 280 285

Leu Ala Ser Ser Ala Val Val Gly Met Arg Phe Asp Ser Ser Glu Gly

290 295 300

Leu Pro Asp Asn Ser Gly Val Leu Val Ala Val Asn Glu Pro Gly Ile

305 310 315 320

Thr Ala Lys Ala Phe Thr Phe Ser Ser Lys Lys Trp Pro His Leu Glu

325 330 335

Ala Arg Gly Gly Ala Leu Val Arg Ala Ser Phe Gly Arg Leu Gly Asp

340 345 350

Glu Ala Ser Ala Arg Met Asp Glu Asp Leu Leu Val Asp Ala Ala Leu

355 360 365

Asp Asp Leu Leu Thr Ile Thr Gly Phe Asp Gly Arg Ala Ala Gly Leu

370 375 380

Gly Glu Ile Phe Val Gln Arg Trp Phe Gly Gly Leu Pro Ala Tyr Gly

385 390 395 400

Val Asp His Ile Ala Thr Val Ser Ala Ala Arg Ala Glu Ile Ala Ala

405 410 415

Val Pro Gly Val Glu Ala Ile Gly Ala Trp Ala Gly Gly Val Gly Val

420 425 430

Pro Ala Val Ile Ala Asp Ala Gln Ala Ala Val His Arg Leu Leu Gly

435 440 445

<210>17

<211>1690

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1660)

<223>RXA00389

<400>17

ccaccactgc gtaacctttc cgagcaagat atcgcggacc tgtcggattt gcttgccacc 60

tctggcgcag gttcctaccg ccttcagttg aggtgaaagc atg atc acc gca acc 115

Met Ile Thr Ala Thr

1 5

gca ctg cat ggg tgt tca ctg att gat ggc gag tgg gtc gct gga aaa 163

Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu Trp Val Ala Gly Lys

10 15 20

aat ggt gag att aca gga ttc gat ccg cgc acc aat gcg agt ctg aac 211

Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr Asn Ala Ser Leu Asn

25 30 35

cct tcc tac tct tta gca aac agc gca cag ctg cgc gcc gcc aca aca 259

Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu Arg Ala Ala Thr Thr

40 45 50

tcg gcg aag cga gct ttt gaa agc tac cga ctc act act cca gag gtt 307

Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu Thr Thr Pro Glu Val

55 60 65

aga gca gat ttc ctg gat tcc atc gct gac aac atc gat gcg cta tcc 355

Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn Ile Asp Ala Leu Ser

70 75 80 85

ggc gag atc gtg caa cgg gcg agc ctg gag aca ggt ttg gga act acc 403

Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr Gly Leu Gly Thr Thr

90 95 100

cga ctc aca ggc gaa gta gcc cgc acc agc aac cag ctc cgc ctg ttt 451

Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn Gln Leu Arg Leu Phe

105 110 115

gca gaa acc gtg aga agc gga cag ttc cac cga gta cgc att gaa cga 499

Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg Val Arg Ile Glu Arg

120 125 130

gga ccg cgg att gat ctt cgc cag cgt cag gtt ccg ttg gga cca gtc 547

Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val Pro Leu Gly Pro Val

135 140 145

gcg gta ttc ggg gca agc aac ttc ccc gtc gct ttc tct act gct ggt 595

Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala Phe Ser Thr Ala Gly

150 155 160 165

ggc gat aca gca tca gcg ttg gct gca ggc tgc cct gtg gtt ttt aag 643

Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys Pro Val Val Phe Lys

170 175 180

gcg cat aat gcg cac cct gga aca gct gag ctc gtc ggg caa gcg gtg 691

Ala His Asn Ala His Pro Gly Thr Ala Glu Leu Val Gly Gln Ala Val

185 190 195

cgg gga gcc gtc gaa aag cat gag ttt gat gct ggt gtg ttt aac ctt 739

Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala Gly Val Phe Asn Leu

200 205 210

gtc tac ggc cgt ggc gtg gaa att ggc cag gag ctg gct gcg gat ccg 787

Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu Leu Ala Ala Asp Pro

215 220 225

aat atc acg gca atc ggt ttt acc ggt tca cgc cag ggt ggt ttg gca 835

Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg Gln Gly Gly Leu Ala

230 235 240 245

ctg tca cag act gcg ttt agc cgc cca gtt ccc gtt cca gtc ttt gca 883

Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro Val Pro Val Phe Ala

250 255 260

gaa atg agt gcc acc aac cct gtg ttc gtc ttc ccc ggc gcg ctg gcg 931

Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe Pro Gly Ala Leu Ala

265 270 275

gat ttg gat gca tcg agt tcc ttg gct gag gcg ttt acc gct tcc gtc 979

Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala Phe Thr Ala Ser Val

280 285 290

acc ggc agt tcc ggg caa ttg tgc acc aag cct ggc ctc gtt ttc atc 1027

Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro Gly Leu Val Phe Ile

295 300 305

ccg cgc ggt gtt gtt ggt gat gct ttt gtg gcg ctc gta gca gcc aaa 1075

Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala Leu Val Ala Ala Lys

310 315 320 325

ttt aaa gaa acc acg ggt caa acg atg ctc acg caa ggc atc gct cag 1123

Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr Gln Gly Ile Ala Gln

330 335 340

gca tgg cag cgc gga gtc gac aac ctt gca gca cag cca agt gta aaa 1171

Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala Gln Pro Ser Val Lys

345 350 355

atc ctc gcc caa ggc acc ccc gga gat gga gag aac gcg ccg ggc ccg 1219

Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu Asn Ala Pro Gly Pro

360 365 370

gtg gtg ttt gaa agt gat gtg cag gcg ttg cta aat aat gtg gtg ttg 1267

Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu Asn Asn Val Val Leu

375 380 385

cag gaa gaa atc ttc ggt gcg gca tcg ctg gtg gtg cgt tat gat tcc 1315

Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val Val Arg Tyr Asp Ser

390 395 400 405

ccg gat caa ctc cac caa gta gcc aat tca ctc gag gga caa tta aca 1363

Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu Glu Gly Gln Leu Thr

410 415 420

gcc acg atc cac gca tcc cag gat gat ttc cag gaa gtc tcg aaa ctt 1411

Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln Glu Val Ser Lys Leu

425 430 435

atc ccc ctc ttg gag gat ctc gcg ggc cgt gtt ctt tac ggc ggc tgg 1459

Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val Leu Tyr Gly Gly Trp

440 445 450

cca acg ggt gtg gaa gtt ggg cac acg gtt atc cat gga ggc cct tat 1507

Pro Thr Gly Val Glu Val Gly His Thr Val Ile His Gly Gly Pro Tyr

455 460 465

ccg gcg acc tca aat gcg cag tcg aca agt gtt gga acc ctg gca atc 1555

Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val Gly Thr Leu Ala Ile

470 475 480 485

gag aga ttt atg cgc ccg gtt tct tat caa act ttc ccg gct gag ctg 1603

Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr Phe Pro Ala Glu Leu

490 495 500

ctt cca gat cca gtt tct gag gcg aat aaa tgg gct gta cct cgg gaa 1651

Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp Ala Val Pro Arg Glu

505 510 515

ata gac cgt taatagctgg tctttacatt tgccagaaag 1690

Ile Asp Arg

520

<210>18

<211>520

<212>PRT

<213>谷氨酸棒状杆菌

<400>18

Met Ile Thr Ala Thr Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu

1 5 10 15

Trp Val Ala Gly Lys Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr

20 25 30

Asn Ala Ser Leu Asn Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu

35 40 45

Arg Ala Ala Thr Thr Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu

50 55 60

Thr Thr Pro Glu Val Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn

65 70 75 80

Ile Asp Ala Leu Ser Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr

85 90 95

Gly Leu Gly Thr Thr Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn

100 105 110

Gln Leu Arg Leu Phe Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg

115 120 125

Val Arg Ile Glu Arg Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val

130 135 140

Pro Leu Gly Pro Val Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala

145 150 155 160

Phe Ser Thr Ala Gly Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys

165 170 175

Pro Val Val Phe Lys Ala His Asn Ala His Pro Gly Thr Ala Glu Leu

180 185 190

Val Gly Gln Ala Val Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala

195 200 205

Gly Val Phe Asn Leu Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu

210 215 220

Leu Ala Ala Asp Pro Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg

225 230 235 240

Gln Gly Gly Leu Ala Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro

245 250 255

Val Pro Val Phe Ala Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe

260 265 270

Pro Gly Ala Leu Ala Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala

275 280 285

Phe Thr Ala Ser Val Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro

290 295 300

Gly Leu Val Phe Ile Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala

305 310 315 320

Leu Val Ala Ala Lys Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr

325 330 335

Gln Gly Ile Ala Gln Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala

340 345 350

Gln Pro Ser Val Lys Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu

355 360 365

Asn Ala Pro Gly Pro Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu

370 375 380

Asn Asn Val Val Leu Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val

385 390 395 400

Val Arg Tyr Asp Ser Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu

405 410 415

Glu Gly Gln Leu Thr Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln

420 425 430

Glu Val Ser Lys Leu Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val

435 440 445

Leu Tyr Gly Gly Trp Pro Thr Gly Val Glu Val Gly His Thr Val Ile

450 455 460

His Gly Gly Pro Tyr Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val

465 470 475 480

Gly Thr Leu Ala Ile Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr

485 490 495

Phe Pro Ala Glu Leu Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp

500 505 510

Ala Val Pro Arg Glu Ile Asp Arg

515 520

<210>19

<211>2161

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2131)

<223>RXA00416

<400>19

caagctctga aagcagatag cacgtgagac tcattaatac atacttgtaa ttttgctcct 60

cgatcccagc aatgctcgtt gagtatgaaa gacttctatc atg att aac acc tct 115

Met Ile Asn Thr Ser

1 5

gaa gat gaa ggt gca gtc gtg gtt cat gag gat ttg aat cct tat ggt 163

Glu Asp Glu Gly Ala Val Val Val His Glu Asp Leu Asn Pro Tyr Gly

10 15 20

cca tca cgt gtg cct gaa ggc gaa gtg tat cgg cct gat cgt cta aac 211

Pro Ser Arg Val Pro Glu Gly Glu Val Tyr Arg Pro Asp Arg Leu Asn

25 30 35

agg aaa gct aca ctt gtt gca att gtg gga gct gcc atc ctg gct ttc 259

Arg Lys Ala Thr Leu Val Ala Ile Val Gly Ala Ala Ile Leu Ala Phe

40 45 50

gca ttt gct ttg gtg ttg tgg atg ggt tta aaa caa aca aac ctt cca 307

Ala Phe Ala Leu Val Leu Trp Met Gly Leu Lys Gln Thr Asn Leu Pro

55 60 65

gct ttc ggc cca tcg aat gtc acg agg gct gtt gct tct gcc acc atc 355

Ala Phe Gly Pro Ser Asn Val Thr Arg Ala Val Ala Ser Ala Thr Ile

70 75 80 85

gct gcg gtt ttg att gtc acg ggt ttt ttg acg tgg ctg tgg ctg cgc 403

Ala Ala Val Leu Ile Val Thr Gly Phe Leu Thr Trp Leu Trp Leu Arg

90 95 100

gat gaa cat cag agc aat ccg cgg tgg gag ttg gag gac gtc aag cca 451

Asp Glu His Gln Ser Asn Pro Arg Trp Glu Leu Glu Asp Val Lys Pro

105 110 115

agg ccc aag tgg cgg acg gcg ctg acg tat ctg gcg tct tac tta agc 499

Arg Pro Lys Trp Arg Thr Ala Leu Thr Tyr Leu Ala Ser Tyr Leu Ser

120 125 130

cca gct gcg ctg gtg gtt gcg gtg ttg gct att ccg ctg tct gcg acc 547

Pro Ala Ala Leu Val Val Ala Val Leu Ala Ile Pro Leu Ser Ala Thr

135 140 145

cgc ctg tat ttg gac gga atc agc gtt gac cag ggc ttt aga act cag 595

Arg Leu Tyr Leu Asp Gly Ile Ser Val Asp Gln Gly Phe Arg Thr Gln

150 155 160 165

ttt tta acc cgc atg gct gac gat atc ggc ttg tcg gac atg aac tac 643

Phe Leu Thr Arg Met Ala Asp Asp Ile Gly Leu Ser Asp Met Asn Tyr

170 175 180

atc gat atg cct acc ttc tac cct gct gga tgg ttc tgg ctc ggt ggt 691

Ile Asp Met Pro Thr Phe Tyr Pro Ala Gly Trp Phe Trp Leu Gly Gly

185 190 195

cgc ttg gcc aat ctt ttg ggg ctg ccc ggt tgg gaa gct ttc cag cca 739

Arg Leu Ala Asn Leu Leu Gly Leu Pro Gly Trp Glu Ala Phe Gln Pro

200 205 210

tgg gca att gtg tcc atg gca gtt gct gct tct gtg tta gtt cca gtg 787

Trp Ala Ile Val Ser Met Ala Val Ala Ala Ser Val Leu Val Pro Val

215 220 225

tgg cag cgc atc acc ggt tcc ctg ccg gtg gca aca ggc att gcg ttg 835

Trp Gln Arg Ile Thr Gly Ser Leu Pro Val Ala Thr Gly Ile Ala Leu

230 235 240 245

gtg aca acc tgc att atc ttg gcg atg aat tcc gaa gag ccc tac gct 883

Val Thr Thr Cys Ile Ile Leu Ala Met Asn Ser Glu Glu Pro Tyr Ala

250 255 260

gca atc gtt gcg atg ggt att cca gcg atg ctc gtg ctg gct tcc cgc 931

Ala Ile Val Ala Met Gly Ile Pro Ala Met Leu Val Leu Ala Ser Arg

265 270 275

att gcc aag ggc gat aag ttt gcg ctt gcc ggc ggc att att tac ttg 979

Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala Gly Gly Ile Ile Tyr Leu

280 285 290

ggt gtt tcg gct act ttc tat act ttg ttc acc ggt gct atc gcg ctt 1027

Gly Val Ser Ala Thr Phe Tyr Thr Leu Phe Thr Gly Ala Ile Ala Leu

295 300 305

tct gcg gtc gcg gtg tgc atc gtg gtg gcg gct att gtg cag cgc tcc 1075

Ser Ala Val Ala Val Cys Ile Val Val Ala Ala Ile Val Gln Arg Ser

310 315 320 325

atc aaa cca ctg ctg tgg ctt gca gtg ctg ggt ggt gga tcc att gtc 1123

Ile Lys Pro Leu Leu Trp Leu Ala Val Leu Gly Gly Gly Ser Ile Val

330 335 340

att gcg ttg att tct tgg ggt cct tac ctt ctg gcc tcc atc aac gga 1171

Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu Leu Ala Ser Ile Asn Gly

345 350 355

gcg gag cgc tct ggc gat tcc gca aca cac tac ctg cct ctt gaa ggc 1219

Ala Glu Arg Ser Gly Asp Ser Ala Thr His Tyr Leu Pro Leu Glu Gly

360 365 370

acc caa ttc ccg gtt cct ttc ttg gca tca agc gtt gtg gga ctg ttg 1267

Thr Gln Phe Pro Val Pro Phe Leu Ala Ser Ser Val Val Gly Leu Leu

375 380 385

tgt ctt gtt ggc ctg atc tat ttg gtg gtg cgt ttc cac aac aat gag 1315

Cys Leu Val Gly Leu Ile Tyr Leu Val Val Arg Phe His Asn Asn Glu

390 395 400 405

gtg cgc gcg atg tgg gtc ggc atc gca gtg ttt tat gcc tgg atg ggc 1363

Val Arg Ala Met Trp Val Gly Ile Ala Val Phe Tyr Ala Trp Met Gly

410 415 420

atg tcc atg gcg atc acg ctt ttg ggc aac acg ttg ctt gga ttc cgt 1411

Met Ser Met Ala Ile Thr Leu Leu Gly Asn Thr Leu Leu Gly Phe Arg

425 430 435

ctt gat acg gtg ctg gtg ctt att ttt gcc acg gct gga gtg ttg ggc 1459

Leu Asp Thr Val Leu Val Leu Ile Phe Ala Thr Ala Gly Val Leu Gly

440 445 450

att gca gat ttc cgc ctt gcc agt gtg tat cag ctc tac ccc acc caa 1507

Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr Gln Leu Tyr Pro Thr Gln

455 460 465

atc aca gag cgc acg gcc acc cat ctg acc aat cta att gtg gtc ctc 1555

Ile Thr Glu Arg Thr Ala Thr His Leu Thr Asn Leu Ile Val Val Leu

470 475 480 485

gtg ctg ctt ggc ggc ctc tac tac gcg caa gat ctg ccg cag aag aac 1603

Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gln Asp Leu Pro Gln Lys Asn

490 495 500

gca cga gct atc gat ctg gcc tat acc gat act gat ggc tac ggc gag 1651

Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp Thr Asp Gly Tyr Gly Glu

505 510 515

cgc gcg gat ctg tat ccg gcc gga gct gca cgt tat tac aag gac atc 1699

Arg Ala Asp Leu Tyr Pro Ala Gly Ala Ala Arg Tyr Tyr Lys Asp Ile

520 525 530

aac gat cat ctg ctt gat caa gga ttc gag cct tcc gaa act gtc gtg 1747

Asn Asp His Leu Leu Asp Gln Gly Phe Glu Pro Ser Glu Thr Val Val

535 540 545

ctg aca gac gaa ctc gat ttc atg tcc tac tac cct tat cgc gga tac 1795

Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr Tyr Pro Tyr Arg Gly Tyr

550 555 560 565

caa gct ttt act tcc cac tac gcc aac ccg ctt ggt gag ttc gga aac 1843

Gln Ala Phe Thr Ser His Tyr Ala Asn Pro Leu Gly Glu Phe Gly Asn

570 575 580

agg aac gca ttc atc gaa gat ctc gcg atc cga agc tgg gat gag ttg 1891

Arg Asn Ala Phe Ile Glu Asp Leu Ala Ile Arg Ser Trp Asp Glu Leu

585 590 595

gct gat cct caa caa ttc agc gac gcc ttg aac acc tct cca tgg acg 1939

Ala Asp Pro Gln Gln Phe Ser Asp Ala Leu Asn Thr Ser Pro Trp Thr

600 605 610

atc cct gag gtg ttc atc ttc cgt ggc tcc atc gat gat cct gac gcc 1987

Ile Pro Glu Val Phe Ile Phe Arg Gly Ser Ile Asp Asp Pro Asp Ala

615 620 625

ggt tgg aaa tac gac gtg gct gaa gat ctg tac ccg aac aat cca aac 2035

Gly Trp Lys Tyr Asp Val Ala Glu Asp Leu Tyr Pro Asn Asn Pro Asn

630 635 640 645

gtg cgc ttc cgc ggc gtg tac ttt aac ccg gag tca ttt gat cag atg 2083

Val Arg Phe Arg Gly Val Tyr Phe Asn Pro Glu Ser Phe Asp Gln Met

650 655 660

tgg cag acc aag caa gtg gga cct ttc gtg gtg gta acg cac aat gag 2131

Trp Gln Thr Lys Gln Val Gly Pro Phe Val Val Val Thr His Asn Glu

665 670 675

taattcctca ccaaacgacc caagccctat 2161

<210>20

<211>677

<212>PRT

<213>谷氨酸棒状杆菌

<400>20

Met Ile Asn Thr Ser Glu Asp Glu Gly Ala Val Val Val His Glu Asp

1 5 10 15

Leu Asn Pro Tyr Gly Pro Ser Arg Val Pro Glu Gly Glu Val Tyr Arg

20 25 30

Pro Asp Arg Leu Asn Arg Lys Ala Thr Leu Val Ala Ile Val Gly Ala

35 40 45

Ala Ile Leu Ala Phe Ala Phe Ala Leu Val Leu Trp Met Gly Leu Lys

50 55 60

Gln Thr Asn Leu Pro Ala Phe Gly Pro Ser Asn Val Thr Arg Ala Val

65 70 75 80

Ala Ser Ala Thr Ile Ala Ala Val Leu Ile Val Thr Gly Phe Leu Thr

85 90 95

Trp Leu Trp Leu Arg Asp Glu His Gln Ser Asn Pro Arg Trp Glu Leu

100 105 110

Glu Asp Val Lys Pro Arg Pro Lys Trp Arg Thr Ala Leu Thr Tyr Leu

115 120 125

Ala Ser Tyr Leu Ser Pro Ala Ala Leu Val Val Ala Val Leu Ala Ile

130 135 140

Pro Leu Ser Ala Thr Arg Leu Tyr Leu Asp Gly Ile Ser Val Asp Gln

145 150 155 160

Gly Phe Arg Thr Gln Phe Leu Thr Arg Met Ala Asp Asp Ile Gly Leu

165 170 175

Ser Asp Met Asn Tyr Ile Asp Met Pro Thr Phe Tyr Pro Ala Gly Trp

180 185 190

Phe Trp Leu Gly Gly Arg Leu Ala Asn Leu Leu Gly Leu Pro Gly Trp

195 200 205

Glu Ala Phe Gln Pro Trp Ala Ile Val Ser Met Ala Val Ala Ala Ser

210 215 220

Val Leu Val Pro Val Trp Gln Arg Ile Thr Gly Ser Leu Pro Val Ala

225 230 235 240

Thr Gly Ile Ala Leu Val Thr Thr Cys Ile Ile Leu Ala Met Asn Ser

245 250 255

Glu Glu Pro Tyr Ala Ala Ile Val Ala Met Gly Ile Pro Ala Met Leu

260 265 270

Val Leu Ala Ser Arg Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala Gly

275 280 285

Gly Ile Ile Tyr Leu Gly Val Ser Ala Thr Phe Tyr Thr Leu Phe Thr

290 295 300

Gly Ala Ile Ala Leu Ser Ala Val Ala Val Cys Ile Val Val Ala Ala

305 310 315 320

Ile Val Gln Arg Ser Ile Lys Pro Leu Leu Trp Leu Ala Val Leu Gly

325 330 335

Gly Gly Ser Ile Val Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu Leu

340 345 350

Ala Ser Ile Asn Gly Ala Glu Arg Ser Gly Asp Ser Ala Thr His Tyr

355 360 365

Leu Pro Leu Glu Gly Thr Gln Phe Pro Val Pro Phe Leu Ala Ser Ser

370 375 380

Val Val Gly Leu Leu Cys Leu Val Gly Leu Ile Tyr Leu Val Val Arg

385 390 395 400

Phe His Asn Asn Glu Val Arg Ala Met Trp Val Gly Ile Ala Val Phe

405 410 415

Tyr Ala Trp Met Gly Met Ser Met Ala Ile Thr Leu Leu Gly Asn Thr

420 425 430

Leu Leu Gly Phe Arg Leu Asp Thr Val Leu Val Leu Ile Phe Ala Thr

435 440 445

Ala Gly Val Leu Gly Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr Gln

450 455 460

Leu Tyr Pro Thr Gln Ile Thr Glu Arg Thr Ala Thr His Leu Thr Asn

465 470 475 480

Leu Ile Val Val Leu Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gln Asp

485 490 495

Leu Pro Gln Lys Asn Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp Thr

500 505 510

Asp Gly Tyr Gly Glu Arg Ala Asp Leu Tyr Pro Ala Gly Ala Ala Arg

515 520 525

Tyr Tyr Lys Asp Ile Asn Asp His Leu Leu Asp Gln Gly Phe Glu Pro

530 535 540

Ser Glu Thr Val Val Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr Tyr

545 550 555 560

Pro Tyr Arg Gly Tyr Gln Ala Phe Thr Ser His Tyr Ala Asn Pro Leu

565 570 575

Gly Glu Phe Gly Asn Arg Asn Ala Phe Ile Glu Asp Leu Ala Ile Arg

580 585 590

Ser Trp Asp Glu Leu Ala Asp Pro Gln Gln Phe Ser Asp Ala Leu Asn

595 600 605

Thr Ser Pro Trp Thr Ile Pro Glu Val Phe Ile Phe Arg Gly Ser Ile

610 615 620

Asp Asp Pro Asp Ala Gly Trp Lys Tyr Asp Val Ala Glu Asp Leu Tyr

625 630 635 640

Pro Asn Asn Pro Asn Val Arg Phe Arg Gly Val Tyr Phe Asn Pro Glu

645 650 655

Ser Phe Asp Gln Met Trp Gln Thr Lys Gln Val Gly Pro Phe Val Val

660 665 670

Val Thr His Asn Glu

675

<210>21

<211>1252

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1222)

<223>RXA00489

<400>21

cagcccgatt tcttcattga aatcgggctg tttctggttg tgtcgcgtat ctcgggtaaa 60

gtcttcgtcg tatacgacca tttaagggag gcccgtcaca atg cgt gac cac gtt 115

Met Arg Asp His Val

1 5

gaa atc ggt atc ggc cgt gag gca cga cgc acc tac agc ttg gac gat 163

Glu Ile Gly Ile Gly Arg Glu Ala Arg Arg Thr Tyr Ser Leu Asp Asp

10 15 20

att tct gtc gtt tct agc cgc cgc acc cgt tca tcc aaa gat gtc gac 211

Ile Ser Val Val Ser Ser Arg Arg Thr Arg Ser Ser Lys Asp Val Asp

25 30 35

acc act tgg cat att gac gcc tac aag ttt gat ctg ccg ttc atg aat 259

Thr Thr Trp His Ile Asp Ala Tyr Lys Phe Asp Leu Pro Phe Met Asn

40 45 50

cac cca agt gat gca ttg gca agc cct gag ttt gtc att gaa atg ggc 307

His Pro Ser Asp Ala Leu Ala Ser Pro Glu Phe Val Ile Glu Met Gly

55 60 65

aag cag ggt ggc ctt ggc gtg atc aac gct gag ggt ctg tgg ggt cgc 355

Lys Gln Gly Gly Leu Gly Val Ile Asn Ala Glu Gly Leu Trp Gly Arg

70 75 80 85

cat gct gat ctc gat gag gcg atc gca aag gtg att gct gcg tat gag 403

His Ala Asp Leu Asp Glu Ala Ile Ala Lys Val Ile Ala Ala Tyr Glu

90 95 100

gaa ggc gac cag gct gca gcc act cgc act ctt cag gag ctg cac gca 451

Glu Gly Asp Gln Ala Ala Ala Thr Arg Thr Leu Gln Glu Leu His Ala

105 110 115

gcg cca ctg gat act gag ctg ctg agt gag cgc att gcg cag gtt cgt 499

Ala Pro Leu Asp Thr Glu Leu Leu Ser Glu Arg Ile Ala Gln Val Arg

120 125 130

gat tcc ggt gag atc gtt gct gtg cgc gtg tct cca caa aat gtt cgt 547

Asp Ser Gly Glu Ile Val Ala Val Arg Val Ser Pro Gln Asn Val Arg

135 140 145

gag atc gca cca atc gtc atc aag gca ggt gct gat ctg ctg gtt atc 595

Glu Ile Ala Pro Ile Val Ile Lys Ala Gly Ala Asp Leu Leu Val Ile

150 155 160 165

cag ggc acc ctg atc tct gca gag cac gtc aac acc ggt gga gag gcc 643

Gln Gly Thr Leu Ile Ser Ala Glu His Val Asn Thr Gly Gly Glu Ala

170 175 180

ctg aac cta aag gaa ttc atc ggt tct ttg gat gtt cct gtc atc gct 691

Leu Asn Leu Lys Glu Phe Ile Gly Ser Leu Asp Val Pro Val Ile Ala

185 190 195

ggt ggc gtg aac gat tac acc acc gcg ttg cac atg atg cgt acc ggt 739

Gly Gly Val Asn Asp Tyr Thr Thr Ala Leu His Met Met Arg Thr Gly

200 205 210

gct gtg ggc atc atc gtc ggt ggc ggc gag aac acc aac agc cta gca 787

Ala Val Gly Ile Ile Val Gly Gly Gly Glu Asn Thr Asn Ser Leu Ala

215 220 225

ttg ggc atg gag gta tcc atg gcc act gcg att gct gat gtc gct gcg 835

Leu Gly Met Glu Val Ser Met Ala Thr Ala Ile Ala Asp Val Ala Ala

230 235 240 245

gca cgt cgt gat tac ctg gat gag acc ggt gga cgt tac gtg cac atc 883

Ala Arg Arg Asp Tyr Leu Asp Glu Thr Gly Gly Arg Tyr Val His Ile

250 255 260

att gca gat gga agc att gaa aac tcc ggt gat gta gtc aag gct att 931

Ile Ala Asp Gly Ser Ile Glu Asn Ser Gly Asp Val Val Lys Ala Ile

265 270 275

gcc tgt ggc gca gat gct gtg gtg ctg ggt tca ccg ttg gct cgc gct 979

Ala Cys Gly Ala Asp Ala Val Val Leu Gly Ser Pro Leu Ala Arg Ala

280 285 290

gaa gaa gct gct gga aag ggc tac ttc tgg cca gca gtg gca gcg cac 1027

Glu Glu Ala Ala Gly Lys Gly Tyr Phe Trp Pro Ala Val Ala Ala His

295 300 305

cct cgt ttc cca cgc ggt gtg gtt act gag tcc gtg gac ttg gat gag 1075

Pro Arg Phe Pro Arg Gly Val Val Thr Glu Ser Val Asp Leu Asp Glu

310 315 320 325

gca gca cca agc ttg gag cag att ctg cat ggt ccg tct acg atg ccg 1123

Ala Ala Pro Ser Leu Glu Gln Ile Leu His Gly Pro Ser Thr Met Pro

330 335 340

tgg ggt gtg gaa aac ttc gaa ggt gga tta aag cgt gcg ctg gct aag 1171

Trp Gly Val Glu Asn Phe Glu Gly Gly Leu Lys Arg Ala Leu Ala Lys

345 350 355

tgt ggc tac acc gat ttg aag agc ttc caa aag gta agc ctg cac gtt 1219

Cys Gly Tyr Thr Asp Leu Lys Ser Phe Gln Lys Val Ser Leu His Val

360 365 370

aac taggtgtgtg tactcgcctc ttcgcgttgc 1252

Asn

<210>22

<211>374

<212>PRT

<213>谷氨酸棒状杆菌

<400>22

Met Arg Asp His Val Glu Ile Gly Ile Gly Arg Glu Ala Arg Arg Thr

1 5 10 15

Tyr Ser Leu Asp Asp Ile Ser Val Val Ser Ser Arg Arg Thr Arg Ser

20 25 30

Ser Lys Asp Val Asp Thr Thr Trp His Ile Asp Ala Tyr Lys Phe Asp

35 40 45

Leu Pro Phe Met Asn His Pro Ser Asp Ala Leu Ala Ser Pro Glu Phe

50 55 60

Val Ile Glu Met Gly Lys Gln Gly Gly Leu Gly Val Ile Asn Ala Glu

65 70 75 80

Gly Leu Trp Gly Arg His Ala Asp Leu Asp Glu Ala Ile Ala Lys Val

85 90 95

Ile Ala Ala Tyr Glu Glu Gly Asp Gln Ala Ala Ala Thr Arg Thr Leu

100 105 110

Gln Glu Leu His Ala Ala Pro Leu Asp Thr Glu Leu Leu Ser Glu Arg

115 120 125

Ile Ala Gln Val Arg Asp Ser Gly Glu Ile Val Ala Val Arg Val Ser

130 135 140

Pro Gln Asn Val Arg Glu Ile Ala Pro Ile Val Ile Lys Ala Gly Ala

145 150 155 160

Asp Leu Leu Val Ile Gln Gly Thr Leu Ile Ser Ala Glu His Val Asn

165 170 175

Thr Gly Gly Glu Ala Leu Asn Leu Lys Glu Phe Ile Gly Ser Leu Asp

180 185 190

Val Pro Val Ile Ala Gly Gly Val Asn Asp Tyr Thr Thr Ala Leu His

195 200 205

Met Met Arg Thr Gly Ala Val Gly Ile Ile Val Gly Gly Gly Glu Asn

210 215 220

Thr Asn Ser Leu Ala Leu Gly Met Glu Val Ser Met Ala Thr Ala Ile

225 230 235 240

Ala Asp Val Ala Ala Ala Arg Arg Asp Tyr Leu Asp Glu Thr Gly Gly

245 250 255

Arg Tyr Val His Ile Ile Ala Asp Gly Ser Ile Glu Asn Ser Gly Asp

260 265 270

Val Val Lys Ala Ile Ala Cys Gly Ala Asp Ala Val Val Leu Gly Ser

275 280 285

Pro Leu Ala Arg Ala Glu Glu Ala Ala Gly Lys Gly Tyr Phe Trp Pro

290 295 300

Ala Val Ala Ala His Pro Arg Phe Pro Arg Gly Val Val Thr Glu Ser

305 310 315 320

Val Asp Leu Asp Glu Ala Ala Pro Ser Leu Glu Gln Ile Leu His Gly

325 330 335

Pro Ser Thr Met Pro Trp Gly Val Glu Asn Phe Glu Gly Gly Leu Lys

340 345 350

Arg Ala Leu Ala Lys Cys Gly Tyr Thr Asp Leu Lys Ser Phe Gln Lys

355 360 365

Val Ser Leu His Val Asn

370

<210>23

<211>1066

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1036)

<223>RXA00552

<400>23

ccgccaacaa ggcagcaaag ctcgatccaa ttgacgcctt gcgttatgag taaaagcctc 60

gtttttaagg tagccacaca tcgcactaga ctgaagaact gtg gct acc tca aaa 115

Val Ala Thr Ser Lys

1 5

att ctt ctt tat tac gca ttc acc ccg ctc tct gac cct aaa gcg gtt 163

Ile Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser Asp Pro Lys Ala Val

10 15 20

cag ctg tgg cag cgt gag ctc tgc gag tca ctg aat ctt cgt ggc cgc 211

Gln Leu Trp Gln Arg Glu Leu Cys Glu Ser Leu Asn Leu Arg Gly Arg

25 30 35

atc ctg atc tcc act cac ggc atc aat gga acc gtg ggc gga gat att 259

Ile Leu Ile Ser Thr His Gly Ile Asn Gly Thr Val Gly Gly Asp Ile

40 45 50

gat gat tgc aag gcg tac att aaa aag acc cgc gag tac cca ggt ttc 307

Asp Asp Cys Lys Ala Tyr Ile Lys Lys Thr Arg Glu Tyr Pro Gly Phe

55 60 65

aac cgc atg cag ttt aag tgg tcc gag ggt ggc gct gag gat ttc cca 355

Asn Arg Met Gln Phe Lys Trp Ser Glu Gly Gly Ala Glu Asp Phe Pro

70 75 80 85

aag ctc agt gtc aaa gtc cgc gat gag atc gtt gcc ttc ggc gct cca 403

Lys Leu Ser Val Lys Val Arg Asp Glu Ile Val Ala Phe Gly Ala Pro

90 95 100

gat gag ctc aaa gtg gat gaa aac ggc gtc gtc ggt ggc ggc gtt cac 451

Asp Glu Leu Lys Val Asp Glu Asn Gly Val Val Gly Gly Gly Val His

105 110 115

ctg aaa cca cag cag gtc aat gag ctt gtg gaa gcc cgt ggc gat gaa 499

Leu Lys Pro Gln Gln Val Asn Glu Leu Val Glu Ala Arg Gly Asp Glu

120 125 130

gtt gtg ttc ttt gac ggc cgc aac gca atg gaa gcc cag atc ggc aag 547

Val Val Phe Phe Asp Gly Arg Asn Ala Met Glu Ala Gln Ile Gly Lys

135 140 145

ttc aag gac gct gtt gtc cct gac gta gaa acc act cat gat ttc atc 595

Phe Lys Asp Ala Val Val Pro Asp Val Glu Thr Thr His Asp Phe Ile

150 155 160 165

gca gaa att gag tct gga aaa tac gac gat ctc aaa gac aag cct gtg 643

Ala Glu Ile Glu Ser Gly Lys Tyr Asp Asp Leu Lys Asp Lys Pro Val

170 175 180

gtc acc tac tgc acc ggc gga att cgt tgt gag atc ctg agt tca ctc 691

VaL Thr Tyr Cys Thr Gly Gly Ile Arg Cys Glu Ile Leu Ser Ser Leu

185 190 195

atg atc aac cgt ggt ttc aaa gag gtc tac caa atc gat ggc ggc atc 739

Met Ile Asn Arg Gly Phe Lys Glu Val Tyr Gln Ile Asp Gly Gly Ile

200 205 210

gtt cgc tac ggc gag cag ttt ggc aac aag ggc ctg tgg gaa ggc tcc 787

Val Arg Tyr Gly Glu Gln Phe Gly Asn Lys Gly Leu Trp Glu Gly Ser

215 220 225

ctc tac gtt ttc gat aag cgc atg cat atg gaa ttc ggc gag gat tac 835

Leu Tyr Val Phe Asp Lys Arg Met His Met Glu Phe Gly Glu Asp Tyr

230 235 240 245

aaa gag gtc gga cac tgc atc cat tgc gat act ccc acc aac aaa ttt 883

Lys Glu Val Gly His Cys Ile His Cys Asp Thr Pro Thr Asn Lys Phe

250 255 260

gag cac tgc ctc aac gaa gat gat tgc cgc gag ctc gtg ttg atg tgc 931

Glu His Cys Leu Asn Glu Asp Asp Cys Arg Glu Leu Val Leu Met Cys

265 270 275

cct gat tgc ttc gcc aat gtt gag acc cgt cat tgc aag cgc gaa cgc 979

Pro Asp Cys Phe Ala Asn Val Glu Thr Arg His Cys Lys Arg Glu Arg

280 285 290

tgt gca gca att gct gcg gat ttc gct gag caa gga att gat ccg ctc 1027

Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu Gln Gly Ile Asp Pro Leu

295 300 305

gtt act tct taaaaagggt atggtggctg ggtatgagca 1066

Val Thr Ser

310

<210>24

<211>312

<212>PRT

<213>谷氨酸棒状杆菌

<400>24

Val Ala Thr Ser Lys Ile Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser

1 5 10 15

Asp Pro Lys Ala Val Gln Leu Trp Gln Arg Glu Leu Cys Glu Ser Leu

20 25 30

Asn Leu Arg Gly Arg Ile Leu Ile Ser Thr His Gly Ile Asn Gly Thr

35 40 45

Val Gly Gly Asp Ile Asp Asp Cys Lys Ala Tyr Ile Lys Lys Thr Arg

50 55 60

Glu Tyr Pro Gly Phe Asn Arg Met Gln Phe Lys Trp Ser Glu Gly Gly

65 70 75 80

Ala Glu Asp Phe Pro Lys Leu Ser Val Lys Val Arg Asp Glu Ile Val

85 90 95

Ala Phe Gly Ala Pro Asp Glu Leu Lys Val Asp Glu Asn Gly Val Val

100 105 110

Gly Gly Gly Val His Leu Lys Pro Gln Gln Val Asn Glu Leu Val Glu

115 120 125

Ala Arg Gly Asp Glu Val Val Phe Phe Asp Gly Arg Asn Ala Met Glu

130 135 140

Ala Gln Ile Gly Lys Phe Lys Asp Ala Val Val Pro Asp Val Glu Thr

145 150 155 160

Thr His Asp Phe Ile Ala Glu Ile Glu Ser Gly Lys Tyr Asp Asp Leu

165 170 175

Lys Asp Lys Pro Val Val Thr Tyr Cys Thr Gly Gly Ile Arg Cys Glu

180 185 190

Ile Leu Ser Ser Leu Met Ile Asn Arg Gly Phe Lys Glu Val Tyr Gln

195 200 205

Ile Asp Gly Gly Ile Val Arg Tyr Gly Glu Gln Phe Gly Asn Lys Gly

210 215 220

Leu Trp Glu Gly Ser Leu Tyr Val Phe Asp Lys Arg Met His Met Glu

225 230 235 240

Phe Gly Glu Asp Tyr Lys Glu Val Gly His Cys Ile His Cys Asp Thr

245 250 255

Pro Thr Asn Lys Phe Glu His Cys Leu Asn Glu Asp Asp Cys Arg Glu

260 265 270

Leu Val Leu Met Cys Pro Asp Cys Phe Ala Asn Val Glu Thr Arg His

275 280 285

Cys Lys Arg Glu Arg Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu Gln

290 295 300

Gly Ile Asp Pro Leu Val Thr Ser

305 310

<210>25

<211>2005

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1975)

<223>RXA00579

<400>25

tggaagactg gaccatcgtc taagtttttc tttgagtttt catatgtaga aggcatcgtc 60

ggcttcggcc tggcggtgct tttctcgttg ttttgtggtt ttg tca gag gat gtc 115

Leu Ser Glu Asp Val

1 5

atg cgc gtt tta att att gat aat tat gat tct ttc acg ttt aat ctc 163

Met Arg Val Leu Ile Ile Asp Asn Tyr Asp Ser Phe Thr Phe Asn Leu

10 15 20

gcc acc tat gtg gaa gag gtt acg ggt cag gca cct gtg gtg gtg cct 211

Ala Thr Tyr Val Glu Glu Val Thr Gly Gln Ala Pro Val Val Val Pro

25 30 35

aat gat caa gaa ata gat gag atg ctt ttc gac gcc gtc atc ctc tca 259

Asn Asp Gln Glu Ile Asp Glu Met Leu Phe Asp Ala Val Ile Leu Ser

40 45 50

cct ggc ccg ggc cac gcc ggc gtt gcg gct gat ttt ggt atc tgt gca 307

Pro Gly Pro Gly His Ala Gly Val Ala Ala Asp Phe Gly Ile Cys Ala

55 60 65

ggc gtc att gag cgt gca cgc gtt ccg att ttg ggt gtg tgt tta ggc 355

Gly Val Ile Glu Arg Ala Arg Val Pro Ile Leu Gly Val Cys Leu Gly

70 75 80 85

cac cag ggc att gcg ttg gcc tat ggc ggt gat gtt gat ttg gcg ccc 403

His Gln Gly Ile Ala Leu Ala Tyr Gly Gly Asp Val Asp Leu Ala Pro

90 95 100

agg ccg gtc cac ggt gag gtt tcg cag atc acc cat gat ggt tca ggt 451

Arg Pro Val His Gly Glu Val Ser Gln Ile Thr His Asp Gly Ser Gly

105 110 115

tta ttt gca ggc atc cct gaa acg ttt gag gcg gtg cgt tat cac tcg 499

Leu Phe Ala Gly Ile Pro Glu Thr Phe Glu Ala Val Arg Tyr His Ser

120 125 130

atg gtg gca acc cgc ttg ccg gag tca ttg aaa gct aca gct acc agc 547

Met Val Ala Thr Arg Leu Pro Glu Ser Leu Lys Ala Thr Ala Thr Ser

135 140 145

gat gat ggt ttg atc atg gca ttg gca cat gaa gtg ctt ccg cag tgg 595

Asp Asp Gly Leu Ile Met Ala Leu Ala His Glu Val Leu Pro Gln Trp

150 155 160 165

ggt gtg caa ttt cat ccg gaa tct att ggt gga caa ttc ggc cat cag 643

Gly Val Gln Phe His Pro Glu Ser Ile Gly Gly Gln Phe Gly His Gln

170 175 180

atc att aag aac ttc ctt aat tta gcg cgc aca tat cgc tgg caa ctc 691

Ile Ile Lys Asn Phe Leu Asn Leu Ala Arg Thr Tyr Arg Trp Gln Leu

185 190 195

acg gag aaa act att ccg ctc agc gtt gat tca gca gcg gtt ttt gaa 739

Thr Glu Lys Thr Ile Pro Leu Ser Val Asp Ser Ala Ala Val Phe Glu

200 205 210

aca ttc ttt gcc cat tcc tcc cat gct ttt tgg ctc gat gat gcc caa 787

Thr Phe Phe Ala His Ser Ser His Ala Phe Trp Leu Asp Asp Ala Gln

215 220 225

gga acc agc tat ctt ggt gat gcc agc ggt cct ctc gca cgc aca aaa 835

Gly Thr Ser Tyr Leu Gly Asp Ala Ser Gly Pro Leu Ala Arg Thr Lys

230 235 240 245

acc cat aat gtc ggc gag ggg gat ttc ttc acc tgg cta aag gag gat 883

Thr His Asn Val Gly Glu Gly Asp Phe Phe Thr Trp Leu Lys Glu Asp

250 255 260

ctc gcc gcc aac tca gtt gcg ccc ggt caa ggt ttt cgt ctt ggc tgg 931

Leu Ala Ala Asn Ser Val Ala Pro Gly Gln Gly Phe Arg Leu Gly Trp

265 270 275

gtt ggt tac gtt ggt tat gag ctt aaa gcg gaa gct ggc gca cgg gct 979

Val Gly Tyr Val Gly Tyr Glu Leu Lys Ala Glu Ala Gly Ala Arg Ala

280 285 290

gcg cac act tcg agt ctt ccg gat gcg cac ctc att ttt gcc gat cgc 1027

Ala His Thr Ser Ser Leu Pro Asp Ala His Leu Ile Phe Ala Asp Arg

295 300 305

gcc atc gca gtg gaa tcg gat cag gtt cgg ttg ctg gcg ttg ggg gag 1075

Ala Ile Ala Val Glu Ser Asp Gln Val Arg Leu Leu Ala Leu Gly Glu

310 315 320 325

cag gac gag tgg ttt gaa gaa acc atc aag aag ctg cat aat ctt gtc 1123

Gln Asp Glu Trp Phe Glu Glu Thr Ile Lys Lys Leu His Asn Leu Val

330 335 340

gcc ccg cgg ata cct gcg tcc gga cac ctc gct ttg cag gtt cga gat 117l

Ala Pro Arg Ile Pro Ala Ser Gly His Leu Ala Leu Gln Val Arg Asp

345 350 355

tcc aaa gat gag tat ctc gac aaa att cgc aga gcc cag gag ctg att 1219

Ser Lys Asp Glu Tyr Leu Asp Lys Ile Arg Arg Ala Gln Glu Leu Ile

360 365 370

act cgc ggc gaa tcg tat gaa atc tgc ctg acc aca aaa ctt cag ggc 1267

Thr Arg Gly Glu Ser Tyr Glu Ile Cys Leu Thr Thr Lys Leu Gln Gly

375 380 385

acc act gat gtg gcc cct ctg gct gcc tat cta gca ctg cgt ggg gcc 1315

Thr Thr Asp Val Ala Pro Leu Ala Ala Tyr Leu Ala Leu Arg Gly Ala

390 395 400 405

aat ccc acc gca tat ggt gcg tat ctt cag ctg ggg gat acc tct att 1363

Asn Pro Thr Ala Tyr Gly Ala Tyr Leu Gln Leu Gly Asp Thr Ser Ile

410 415 420

ttg agt tcc tcg ccg gag cgg ttc atc acc att gat tcg gca ggg tat 1411

Leu Ser Ser Ser Pro Glu Arg Phe Ile Thr Ile Asp Ser Ala Gly Tyr

425 430 435

gtg gaa tca aag ccc att aaa ggc acc agg ccg cgt ggg cga aca gcg 1459

Val Glu Ser Lys Pro Ile Lys Gly Thr Arg Pro Arg Gly Arg Thr Ala

440 445 450

caa gaa gac caa gaa atc att gct gag ctg cgc agt aat cct aaa gat 1507

Gln Glu Asp Gln Glu Ile Ile Ala Glu Leu Arg Ser Asn Pro Lys Asp

455 460 465

cgt gca gaa aac ttg atg atc gtg gat ttg gtc cgc aac gac tta gcc 1555

Arg Ala Glu Asn Leu Met Ile Val Asp Leu Val Arg Asn Asp Leu Ala

470 475 480 485

cgc ggc gct ttg ccc acc aca gtt aaa aca tcc aag ctt ttc gac gtc 1603

Arg Gly Ala Leu Pro Thr Thr Val Lys Thr Ser Lys Leu Phe Asp Val

490 495 500

gaa acc tac gcc aca gtc cac caa ctt gtc agc acc gtc tct gca gag 1651

Glu Thr Tyr Ala Thr Val His Gln Leu Val Ser Thr Val Ser Ala Glu

505 510 515

ttg ggg cca cgc agt ccg att gag tgc gtg cgc gca gca ttc ccc ggt 1699

Leu Gly Pro Arg Ser Pro Ile Glu Cys Val Arg Ala Ala Phe Pro Gly

520 525 530

ggt tcg atg act ggt gcc cca aag ctg cgc acc atg gag atc atc gat 1747

Gly Ser Met Thr Gly Ala Pro Lys Leu Arg Thr Met Glu Ile Ile Asp

535 540 545

gag ctg gag gca gct cct cgc ggt att tac tca ggt ggc ttg gga tat 1795

Glu Leu Glu Ala Ala Pro Arg Gly Ile Tyr Ser Gly Gly Leu Gly Tyr

550 555 560 565

ttt tcc ctc gac ggc gca gtt gat ctc tcc atg gtg atc aga act ctc 1843

Phe Ser Leu Asp Gly Ala Val Asp Leu Ser Met Val Ile Arg Thr Leu

570 575 580

gtc atc cag aac aat cac gtg gag tac gga gtg ggc ggt gca ctt ctt 1891

Val Ile Gln Asn Asn His Val Glu Tyr Gly Val Gly Gly Ala Leu Leu

585 590 595

gct ctg tct gat ccg gag gct gag tgg gag gaa atc cgc gtt aaa tca 1939

Ala Leu Ser Asp Pro Glu Ala Glu Trp Glu Glu Ile Arg Val Lys Ser

600 605 610

cgg cct ctg ctg aat ttg ttt ggg gtt gaa ttc cca tgacgtacct 1985

Arg Pro Leu Leu Asn Leu Phe Gly Val Glu Phe Pro

615 620 625

cgtgtgggac ggtgcaacac 2005

<210>26

<211>625

<212>PRT

<213>谷氨酸棒状杆菌

<400>26

Leu Ser Glu Asp Val Met Arg Val Leu Ile Ile Asp Asn Tyr Asp Ser

1 5 10 15

Phe Thr Phe Asn Leu Ala Thr Tyr Val Glu Glu Val Thr Gly Gln Ala

20 25 30

Pro Val Val Val Pro Asn Asp Gln Glu Ile Asp Glu Met Leu Phe Asp

35 40 45

Ala Val Ile Leu Ser Pro Gly Pro Gly His Ala Gly Val Ala Ala Asp

50 55 60

Phe Gly Ile Cys Ala Gly Val Ile Glu Arg Ala Arg Val Pro Ile Leu

65 70 75 80

Gly Val Cys Leu Gly His Gln Gly Ile Ala Leu Ala Tyr Gly Gly Asp

85 90 95

Val Asp Leu Ala Pro Arg Pro Val His Gly Glu Val Ser Gln Ile Thr

100 105 110

His Asp Gly Ser Gly Leu Phe Ala Gly Ile Pro Glu Thr Phe Glu Ala

115 120 125

Val Arg Tyr His Ser Met Val Ala Thr Arg Leu Pro Glu Ser Leu Lys

130 135 140

Ala Thr Ala Thr Ser Asp Asp Gly Leu Ile Met Ala Leu Ala His Glu

145 150 155 160

Val Leu Pro Gln Trp Gly Val Gln Phe His Pro Glu Ser Ile Gly Gly

165 170 175

Gln Phe Gly His Gln Ile Ile Lys Asn Phe Leu Asn Leu Ala Arg Thr

180 185 190

Tyr Arg Trp Gln Leu Thr Glu Lys Thr Ile Pro Leu Ser Val Asp Ser

195 200 205

Ala Ala Val Phe Glu Thr Phe Phe Ala His Ser Ser His Ala Phe Trp

210 215 220

Leu Asp Asp Ala Gln Gly Thr Ser Tyr Leu Gly Asp Ala Ser Gly Pro

225 230 235 240

Leu Ala Arg Thr Lys Thr His Asn Val Gly Glu Gly Asp Phe Phe Thr

245 250 255

Trp Leu Lys Glu Asp Leu Ala Ala Asn Ser Val Ala Pro Gly Gln Gly

260 265 270

Phe Arg Leu Gly Trp Val Gly Tyr Val Gly Tyr Glu Leu Lys Ala Glu

275 280 285

Ala Gly Ala Arg Ala Ala His Thr Ser Ser Leu Pro Asp Ala His Leu

290 295 300

Ile Phe Ala Asp Arg Ala Ile Ala Val Glu Ser Asp Gln Val Arg Leu

305 310 315 320

Leu Ala Leu Gly Glu Gln Asp Glu Trp Phe Glu Glu Thr Ile Lys Lys

325 330 335

Leu His Asn Leu Val Ala Pro Arg Ile Pro Ala Ser Gly His Leu Ala

340 345 350

Leu Gln Val Arg Asp Ser Lys Asp Glu Tyr Leu Asp Lys Ile Arg Arg

355 360 365

Ala Gln Glu Leu Ile Thr Arg Gly Glu Ser Tyr Glu Ile Cys Leu Thr

370 375 380

Thr Lys Leu Gln Gly Thr Thr Asp Val Ala Pro Leu Ala Ala Tyr Leu

385 390 395 400

Ala Leu Arg Gly Ala Asn Pro Thr Ala Tyr Gly Ala Tyr Leu Gln Leu

405 410 415

Gly Asp Thr Ser Ile Leu Ser Ser Ser Pro Glu Arg Phe Ile Thr Ile

420 425 430

Asp Ser Ala Gly Tyr Val Glu Ser Lys Pro Ile Lys Gly Thr Arg Pro

435 440 445

Arg Gly Arg Thr Ala Gln Glu Asp Gln Glu Ile Ile Ala Glu Leu Arg

450 455 460

Ser Asn Pro Lys Asp Arg Ala Glu Asn Leu Met Ile Val Asp Leu Val

465 470 475 480

Arg Asn Asp Leu Ala Arg Gly Ala Leu Pro Thr Thr Val Lys Thr Ser

485 490 495

Lys Leu Phe Asp Val Glu Thr Tyr Ala Thr Val His Gln Leu Val Ser

500 505 510

Thr Val Ser Ala Glu Leu Gly Pro Arg Ser Pro Ile Glu Cys Val Arg

515 520 525

Ala Ala Phe Pro Gly Gly Ser Met Thr Gly Ala Pro Lys Leu Arg Thr

530 535 540

Met Glu Ile Ile Asp Glu Leu Glu Ala Ala Pro Arg Gly Ile Tyr Ser

545 550 555 560

Gly Gly Leu Gly Tyr Phe Ser Leu Asp Gly Ala Val Asp Leu Ser Met

565 570 575

Val Ile Arg Thr Leu Val Ile Gln Asn Asn His Val Glu Tyr Gly Val

580 585 590

Gly Gly Ala Leu Leu Ala Leu Ser Asp Pro Glu Ala Glu Trp Glu Glu

595 600 605

Ile Arg Val Lys Ser Arg Pro Leu Leu Asn Leu Phe Gly Val Glu Phe

610 615 620

Pro

625

<210>27

<211>1120

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1090)

<223>RXA00620

<400>27

gcttattagc tgtacggctc caaggttctc ttaatctttg ttcgaataaa ctgccagctt 60

aaaaatgtct tttaggtaaa caaaaaattc gggcgaatgg gtg atc cat gtc agg 115

Val Ile His Val Arg

1 5

aag cca gcg gtg aaa ccg gca gtg aaa cca gcg gtg aat gct aaa att 163

Lys Pro Ala Val Lys Pro Ala Val Lys Pro Ala Val Asn Ala Lys Ile

10 15 20

ttc cga aca cac ccg agg ggt cta gac ttg cct aac atg cgt cct gaa 211

Phe Arg Thr His Pro Arg Gly Leu Asp Leu Pro Asn Met Arg Pro Glu

25 30 35

ctc tcc cag tac aag cac ctg tcg gca ggc aag gtc cgt gag atc tac 259

Leu Ser Gln Tyr Lys His Leu Ser Ala Gly Lys Val Arg Glu Ile Tyr

40 45 50

gag atc gac gac aag cac atc ctc atg gtg gct tcc gat cgt atc tct 307

Glu Ile Asp Asp Lys His Ile Leu Met Val Ala Ser Asp Arg Ile Ser

55 60 65

gca tac gat ttc atc ctc gat acc gaa att cca gac aag ggt cga gtg 355

Ala Tyr Asp Phe Ile Leu Asp Thr Glu Ile Pro Asp Lys Gly Arg Val

70 75 80 85

ctc act gcg atg agc cag ttc ttc ttc gac acc atc gat ttt cct aat 403

Leu Thr Ala Met Ser Gln Phe Phe Phe Asp Thr Ile Asp Phe Pro Asn

90 95 100

cac ctt gca ggt ccc gct gat gat cca cgt atc cca gaa gaa gtt ttg 451

His Leu Ala Gly Pro Ala Asp Asp Pro Arg Ile Pro Glu Glu Val Leu

105 110 115

gga cga gca atg gtg tgc aag aag ctc aac atg ctt cct ttt gaa tgc 499

Gly Arg Ala Met Val Cys Lys Lys Leu Asn Met Leu Pro Phe Glu Cys

120 125 130

gtg gtt cgt gga tac ctc act ggc tct gga ctt gtt gaa tac aag cag 547

Val Val Arg Gly Tyr Leu Thr Gly Ser Gly Leu Val Glu Tyr Lys Gln

135 140 145

acc agc tcc gtg tgt gga gtt gag ctc cca gaa ggc ctc gtt gaa tct 595

Thr Ser Ser Val Cys Gly Val Glu Leu Pro Glu Gly Leu Val Glu Ser

150 155 160 165

tct cag ctg cct gag cca atc ttt acc cca gcc acc aag gct gac atc 643

Ser Gln Leu Pro Glu Pro Ile Phe Thr Pro Ala Thr Lys Ala Asp Ile

170 175 180

ggc gac cac gac atc aat gtc tcc ttt gac gtt gtt gaa gaa cgt ctc 691

Gly Asp His Asp Ile Asn Val Ser Phe Asp Val Val Glu Glu Arg Leu

185 190 195

ggc gaa gct cgt gcg aac cag ttg cgc gat gcc tct att gct att tac 739

Gly Glu Ala Arg Ala Asn Gln Leu Arg Asp Ala Ser Ile Ala Ile Tyr

200 205 210

aag gct gct gct gag atc gcc cgt gac cgt ggc gtc atc ctt gcc gac 787

Lys Ala Ala Ala Glu Ile Ala Arg Asp Arg Gly Val Ile Leu Ala Asp

215 220 225

acc aaa ttt gag ttc ggc atc gat gaa gat ggc acc ctc gtg ctt ggt 835

Thr Lys Phe Glu Phe Gly Ile Asp Glu Asp Gly Thr Leu Val Leu Gly

230 235 240 245

gat gaa gtc ctt acc cca gat tcc tcc cgc tac tgg cct ttg gaa ggc 883

Asp Glu Val Leu Thr Pro Asp Ser Ser Arg Tyr Trp Pro Leu Glu Gly

250 255 260

tat gaa gca gga tct gtg caa cca agc ttt gat aag caa ttc gtg cgc 931

Tyr Glu Ala Gly Ser Val Gln Pro Ser Phe Asp Lys Gln Phe Val Arg

265 270 275

aac tgg ctc acc ggc cct aaa tct ggc tgg gac aag gat tcc ggc ttg 979

Asn Trp Leu Thr Gly Pro Lys Ser Gly Trp Asp Lys Asp Ser Gly Leu

280 285 290

gag cca cca gct ctg cca ggt tcc gtt gtt gag gca acc cgc gag cgc 1027

Glu Pro Pro Ala Leu Pro Gly Ser Val Val Glu Ala Thr Arg Glu Arg

295 300 305

tac atc gag gcc tac gag ctg att tct ggt cag aag ttc tgc cag tgg 1075

Tyr Ile Glu Ala Tyr Glu Leu Ile Ser Gly Gln Lys Phe Cys Gln Trp

310 315 320 325

att ggt tct tgc gtc taagctgctt gattttccct aaaccccgga 1120

Ile Gly Ser Cys Val

330

<210>28

<211>330

<212>PRT

<213>谷氨酸棒状杆菌

<400>28

Val Ile His Val Arg Lys Pro Ala Val Lys Pro Ala Val Lys Pro Ala

1 5 10 15

Val Asn Ala Lys Ile Phe Arg Thr His Pro Arg Gly Leu Asp Leu Pro

20 25 30

Asn Met Arg Pro Glu Leu Ser Gln Tyr Lys His Leu Ser Ala Gly Lys

35 40 45

Val Arg Glu Ile Tyr Glu Ile Asp Asp Lys His Ile Leu Met Val Ala

50 55 60

Ser Asp Arg Ile Ser Ala Tyr Asp Phe Ile Leu Asp Thr Glu Ile Pro

65 70 75 80

Asp Lys Gly Arg Val Leu Thr Ala Met Ser Gln Phe Phe Phe Asp Thr

85 90 95

Ile Asp Phe Pro Asn His Leu Ala Gly Pro Ala Asp Asp Pro Arg Ile

100 105 110

Pro Glu Glu Val Leu Gly Arg Ala Met Val Cys Lys Lys Leu Asn Met

115 120 125

Leu Pro Phe Glu Cys Val Val Arg Gly Tyr Leu Thr Gly Ser Gly Leu

130 135 140

Val Glu Tyr Lys Gln Thr Ser Ser Val Cys Gly Val Glu Leu Pro Glu

145 150 155 160

Gly Leu Val Glu Ser Ser Gln Leu Pro Glu Pro Ile Phe Thr Pro Ala

165 170 175

Thr Lys Ala Asp Ile Gly Asp His Asp Ile Asn Val Ser Phe Asp Val

180 185 190

Val Glu Glu Arg Leu Gly Glu Ala Arg Ala Asn Gln Leu Arg Asp Ala

195 200 205

Ser Ile Ala Ile Tyr Lys Ala Ala Ala Glu Ile Ala Arg Asp Arg Gly

210 215 220

Val Ile Leu Ala Asp Thr Lys Phe Glu Phe Gly Ile Asp Glu Asp Gly

225 230 235 240

Thr Leu Val Leu Gly Asp Glu Val Leu Thr Pro Asp Ser Ser Arg Tyr

245 250 255

Trp Pro Leu Glu Gly Tyr Glu Ala Gly Ser Val Gln Pro Ser Phe Asp

260 265 270

Lys Gln Phe Val Arg Asn Trp Leu Thr Gly Pro Lys Ser Gly Trp Asp

275 280 285

Lys Asp Ser Gly Leu Glu Pro Pro Ala Leu Pro Gly Ser Val Val Glu

290 295 300

Ala Thr Arg Glu Arg Tyr Ile Glu Ala Tyr Glu Leu Ile Ser Gly Gln

305 310 315 320

Lys Phe Cys Gln Trp Ile Gly Ser Cys Val

325 330

<210>29

<211>1159

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1129)

<223>RXA00636

<400>29

tcatgggatt cagcaaggcg gccacccgaa ccgtctttgg tggaggagta ggagcgatga 60

tcgatctggc ccgttcgaac ataaggaata ttcctactcc atg atg att gat aca 115

Met Met Ile Asp Thr

1 5

cct gct gtt ctc att gac cgc gag cgc tta act gcc aac att tcc agg 163

Pro Ala Val Leu Ile Asp Arg Glu Arg Leu Thr Ala Asn Ile Ser Arg

10 15 20

atg gca gct cac gcc ggt gcc cat gag att gcc ctg cgt ccg cat gtg 211

Met Ala Ala His Ala Gly Ala His Glu Ile Ala Leu Arg Pro His Val

25 30 35

aaa acg cac aaa atc att gaa att gcg cag atg cag gtc gac gcc ggt 259

Lys Thr His Lys Ile Ile Glu Ile Ala Gln Met Gln Val Asp Ala Gly

40 45 50

gcc cga ggg atc acc tgc gca acc att ggc gag gcg gaa att ttt gcc 307

Ala Arg Gly Ile Thr Cys Ala Thr Ile Gly Glu Ala Glu Ile Phe Ala

55 60 65

ggc gca ggt ttt acg gac atc ttt att gca tat ccg ctg tat cta acc 355

Gly Ala Gly Phe Thr Asp Ile Phe Ile Ala Tyr Pro Leu Tyr Leu Thr

70 75 80 85

gat cat gca gtg caa cgc ctg aac gcg atc ccc gga gaa att tcc att 403

Asp His Ala Val Gln Arg Leu Asn Ala Ile Pro Gly Glu Ile Ser Ile

90 95 100

ggc gtg gat tcg gta gag atg gca cag gcg acg gcg ggt ttg cgg gaa 451

Gly Val Asp Ser Val Glu Met Ala Gln Ala Thr Ala Gly Leu Arg Glu

105 110 115

gat atc aag gct ctg att gaa gtg gat tcg gga cat cgt aga agt gga 499

Asp Ile Lys Ala Leu Ile Glu Val Asp Ser Gly His Arg Arg Ser Gly

120 125 130

gtc acg gcg act gct tca gaa ttg agt cag atc cgc gag gcg ctg ggc 547

Val Thr Ala Thr Ala Ser Glu Leu Ser Gln Ile Arg Glu Ala Leu Gly

135 140 145

agc agg tat gca gga gtg ttt act ttt cct ggg cat tct tat ggc ccg 595

Ser Arg Tyr Ala Gly Val Phe Thr Phe Pro Gly His Ser Tyr Gly Pro

150 155 160 165

gga aat ggt gag cag gca gca gct gat gag ctt cag gct cta aac aac 643

Gly Asn Gly Glu Gln Ala Ala Ala Asp Glu Leu Gln Ala Leu Asn Asn

170 175 180

agc gtc cag cga ctt gct ggc ggc ctg act tct ggc ggt tcc tcg ccg 691

Ser Val Gln Arg Leu Ala Gly Gly Leu Thr Ser Gly Gly Ser Ser Pro

185 190 195

tct gcg cag ttt aca gac gca atc gat gag atg cga cca ggc gtg tat 739

Ser Ala Gln Phe Thr Asp Ala Ile Asp Glu Met Arg Pro Gly Val Tyr

200 205 210

gtg ttt aac gat tcc cag cag atc acc tcg gga gca tgc act gag aag 787

Val Phe Asn Asp Ser Gln Gln Ile Thr Ser Gly Ala Cys Thr Glu Lys

215 220 225

cag gtg gca atg acg gtg ctg tct act gtg gtc agc cga aat gtg tca 835

Gln Val Ala Met Thr Val Leu Ser Thr Val Val Ser Arg Asn Val Ser

230 235 240 245

gat cgt cgg atc att ttg gat gcg gga tcc aaa atc ctc agc act gat 883

Asp Arg Arg Ile Ile Leu Asp Ala Gly Ser Lys Ile Leu Ser Thr Asp

250 255 260

aaa cca gca tgg att gat ggc aat ggt ttt gtt ctg ggg aat cct gaa 931

Lys Pro Ala Trp Ile Asp Gly Asn Gly Phe Val Leu Gly Asn Pro Glu

265 270 275

gcc cga atc tct gct ttg tcg gag cat cac gca acc att ttc tgg cca 979

Ala Arg Ile Ser Ala Leu Ser Glu His His Ala Thr Ile Phe Trp Pro

280 285 290

gat aaa gtg cta ctt cca gta atc ggg gag cag ctc aac atc gtg ccc 1027

Asp Lys Val Leu Leu Pro Val Ile Gly Glu Gln Leu Asn Ile Val Pro

295 300 305

aac cat gcc tgc aac gtg att aat ttg gtg gat gag gtc tac gtt cgg 1075

Asn His Ala Cys Asn Val Ile Asn Leu Val Asp Glu Val Tyr Val Arg

310 315 320 325

gaa gcc gat ggc act ttc cgt acc tgg aag gta gtt gcc cgc ggc aga 1123

Glu Ala Asp Gly Thr Phe Arg Thr Trp Lys Val Val Ala Arg Gly Arg

330 335 340

aac aat tagggaaacc tcttgacctt cacgcgacgt 1159

Asn Asn

<210>30

<211>343

<212>PRT

<213>谷氨酸棒状杆菌

<400>30

Met Met Ile Asp Thr Pro Ala Val Leu Ile Asp Arg Glu Arg Leu Thr

1 5 10 15

Ala Asn Ile Ser Arg Met Ala Ala His Ala Gly Ala His Glu Ile Ala

20 25 30

Leu Arg Pro His Val Lys Thr His Lys Ile Ile Glu Ile Ala Gln Met

35 40 45

Gln Val Asp Ala Gly Ala Arg Gly Ile Thr Cys Ala Thr Ile Gly Glu

50 55 60

Ala Glu Ile Phe Ala Gly Ala Gly Phe Thr Asp Ile Phe Ile Ala Tyr

65 70 75 80

Pro Leu Tyr Leu Thr Asp His Ala Val Gln Arg Leu Asn Ala Ile Pro

85 90 95

Gly Glu Ile Ser Ile Gly Val Asp Ser Val Glu Met Ala Gln Ala Thr

100 105 110

Ala Gly Leu Arg Glu Asp Ile Lys Ala Leu Ile Glu Val Asp Ser Gly

115 120 125

His Arg Arg Ser Gly Val Thr Ala Thr Ala Ser Glu Leu Ser Gln Ile

130 135 140

Arg Glu Ala Leu Gly Ser Arg Tyr Ala Gly Val Phe Thr Phe Pro Gly

145 150 155 160

His Ser Tyr Gly Pro Gly Asn Gly Glu Gln Ala Ala Ala Asp Glu Leu

165 170 175

Gln Ala Leu Asn Asn Ser Val Gln Arg Leu Ala Gly Gly Leu Thr Ser

180 185 190

Gly Gly Ser Ser Pro Ser Ala Gln Phe Thr Asp Ala Ile Asp Glu Met

195 200 205

Arg Pro Gly Val Tyr Val Phe Asn Asp Ser Gln Gln Ile Thr Ser Gly

210 215 220

Ala Cys Thr Glu Lys Gln Val Ala Met Thr Val Leu Ser Thr Val Val

225 230 235 240

Ser Arg Asn Val Ser Asp Arg Arg Ile Ile Leu Asp Ala Gly Ser Lys

245 250 255

Ile Leu Ser Thr Asp Lys Pro Ala Trp Ile Asp Gly Asn Gly Phe Val

260 265 270

Leu Gly Asn Pro Glu Ala Arg Ile Ser Ala Leu Ser Glu His His Ala

275 280 285

Thr Ile Phe Trp Pro Asp Lys Val Leu Leu Pro Val Ile Gly Glu Gln

290 295 300

Leu Asn Ile Val Pro Asn His Ala Cys Asn Val Ile Asn Leu Val Asp

305 310 315 320

Glu Val Tyr Val Arg Glu Ala Asp Gly Thr Phe Arg Thr Trp Lys Val

325 330 335

Val Ala Arg Gly Arg Asn Asn

340

<210>31

<211>937

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(907)

<223>RXA00708

<400>31

cctgcgtatc ggctgccttt ttgaattctt ttcctcctcg aggcctaacc ttcaattcct 60

taccgatccc cttccctgaa gtttcgctaa cctggcgtac atg act ctt tcc ctt 115

Met Thr Leu Ser Leu

1 5

cct cca att ggt ttc ggc acc gtt cat ctt gat ggc gca cct ggc gtt 163

Pro Pro Ile Gly Phe Gly Thr Val His Leu Asp Gly Ala Pro Gly Val

10 15 20

gaa gcc atc gct act gcc att gat gct ggt tac cgc ctc atc gac acc 211

Glu Ala Ile Ala Thr Ala Ile Asp Ala Gly Tyr Arg Leu Ile Asp Thr

25 30 35

gcg tac aac tat gaa aat gaa ggt acc gtg ggc aag gct gtc cgc gag 259

Ala Tyr Asn Tyr Glu Asn Glu Gly Thr Val Gly Lys Ala Val Arg Glu

40 45 50

tcg ggt gtc ccc cgc gag gaa ttg att gtt acc agt aag ctc cct ggc 307

Ser Gly Val Pro Arg Glu Glu Leu Ile Val Thr Ser Lys Leu Pro Gly

55 60 65

cgc ttc cat gct cgc gat cta gga cgc gtc cgc att gag gaa agt cta 355

Arg Phe His Ala Arg Asp Leu Gly Arg Val Arg Ile Glu Glu Ser Leu

70 75 80 85

tac cgc ctc aac tta gat tac atc gat ctc ctc ttg att cac tgg cct 403

Tyr Arg Leu Asn Leu Asp Tyr Ile Asp Leu Leu Leu Ile His Trp Pro

90 95 100

aat ccc agc aag gat ctc tac gtc gag gcg tgg gaa acg ctg att gaa 451

Asn Pro Ser Lys Asp Leu Tyr Val Glu Ala Trp Glu Thr Leu Ile Glu

105 110 115

gtc cgc gat gct ggc ctg gtc aag cac atc gga gtg tct aac ttc ctt 499

Val Arg Asp Ala Gly Leu Val Lys His Ile Gly Val Ser Asn Phe Leu

120 125 130

cca aat cac att gat cgc ctg cgc cgc gaa acc ggt gaa ctg ccg gcc 547

Pro Asn His Ile Asp Arg Leu Arg Arg Glu Thr Gly Glu Leu Pro Ala

135 140 145

gtt aac cag atc gag ttg cac ccc tat ttc ccg cag gtg gag cag gta 595

Val Asn Gln Ile Glu Leu His Pro Tyr Phe Pro Gln Val Glu Gln Val

150 155 160 165

gat ttc cac gat gag ctg ggc atc att acc gag gcc tgg agc ccg ctc 643

AspPhe Hi s Asp Glu Leu Gly Ile Ile Thr Glu Ala Trp Ser Pro Leu

170 175 180

agc aac ggt cgc gga ctc gtc gaa gag cca ttg ctc aag gaa atc ggc 691

Ser Asn Gly Arg Gly Leu Val Glu Glu Pro Leu Leu Lys Glu Ile Gly

185 190 195

gag cgc tac ggg gtc ggc agc ggc gaa atc gcc ctc gct tgg cat cac 739

Glu Arg Tyr Gly Val Gly Ser Gly Glu Ile Ala Leu Ala Trp His His

200 205 210

gcc agg gga atc gtt ccg att cca cgc tcc acc aac ccg gcc agg cag 787

Ala Arg Gly Ile Val Pro Ile Pro Arg Ser Thr Asn Pro Ala Arg Gln

215 220 225

cgc agc aac ttg gag gcg gta aag att tcg ctt atc gac gaa gac gtc 835

Arg Ser Asn Leu Glu Ala Val Lys Ile Ser Leu Ile Asp Glu Asp Val

230 235 240 245

cag gcg att acc gct ttg gcg cgc aaa aac ggc cgg atc aaa gat caa 883

Gln Ala Ile Thr Ala Leu Ala Arg Lys Asn Gly Arg Ile Lys Asp Gln

250 255 260

gat cca gcc gtc tat gaa gaa ttc tagatagtta catcaaggtt ccgaggaggc 937

Asp Pro Ala Val Tyr Glu Glu Phe

265

<210>32

<211>269

<212>PRT

<213>谷氨酸棒状杆菌

<400>32

Met Thr Leu Ser Leu Pro Pro Ile Gly Phe Gly Thr Val His Leu Asp

1 5 10 15

Gly Ala Pro Gly Val Glu Ala Ile Ala Thr Ala Ile Asp Ala Gly Tyr

20 25 30

Arg Leu Ile Asp Thr Ala Tyr Asn Tyr Glu Asn Glu Gly Thr Val Gly

35 40 45

Lys Ala Val Arg Glu Ser Gly Val Pro Arg Glu Glu Leu Ile Val Thr

50 55 60

Ser Lys Leu Pro Gly Arg Phe His Ala Arg Asp Leu Gly Arg Val Arg

65 70 75 80

Ile Glu Glu Ser Leu Tyr Arg Leu Asn Leu Asp Tyr Ile Asp Leu Leu

85 90 95

Leu Ile His Trp Pro Asn Pro Ser Lys Asp Leu Tyr Val Glu Ala Trp

100 105 110

Glu Thr Leu Ile Glu Val Arg Asp Ala Gly Leu Val Lys His Ile Gly

115 120 125

Val Ser Asn Phe Leu Pro Asn His Ile Asp Arg Leu Arg Arg Glu Thr

130 135 140

Gly Glu Leu Pro Ala Val Asn Gln Ile Glu Leu His Pro Tyr Phe Pro

145 150 155 160

Gln Val Glu Gln Val Asp Phe His Asp Glu Leu Gly Ile Ile Thr Glu

165 170 175

Ala Trp Ser Pro Leu Ser Asn Gly Arg Gly Leu Val Glu Glu Pro Leu

180 185 190

Leu Lys Glu Ile Gly Glu Arg Tyr Gly Val Gly Ser Gly Glu Ile Ala

195 200 205

Leu Ala Trp His His Ala Arg Gly Ile Val Pro Ile Pro Arg Ser Thr

210 215 220

Asn Pro Ala Arg Gln Arg Ser Asn Leu Glu Ala Val Lys Ile Ser Leu

225 230 235 240

Ile Asp Glu Asp Val Gln Ala Ile Thr Ala Leu Ala Arg Lys Asn Gly

245 250 255

Arg Ile Lys Asp Gln Asp Pro Ala Val Tyr Glu Glu Phe

260 265

<210>33

<211>1132

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1102)

<223>RXA00727

<400>33

tagctgttta ttttaaagtc cctcccgagc ccatgccgga taaaacaaaa cccgagcgcc 60

tgccaacgca aaatccagcc tcacttaagg ttattgcacc atg gca ggt agt att 115

Met Ala Gly Ser Ile

1 5

ttg ccc atg att gag agt ttc acc cga ttg cga att tcg cag aca ccg 163

Leu Pro Met Ile Glu Ser Phe Thr Arg Leu Arg Ile Ser Gln Thr Pro

10 15 20

cct ctg aaa cgg cta ctg cat agc act ttg cgt ttc ccc aaa atc ccc 211

Pro Leu Lys Arg Leu Leu His Ser Thr Leu Arg Phe Pro Lys Ile Pro

25 30 35

aag cgc gct gta gcg gcg acc gtc ggc atc gtg gca acc tca ttc acc 259

Lys Arg Ala Val Ala Ala Thr Val Gly Ile Val Ala Thr Ser Phe Thr

40 45 50

ttg gct tct tgt gtc acc aat gag gag cag ggc aac cca gat ggc tgg 307

Leu Ala Ser Cys Val Thr Asn Glu Glu Gln Gly Asn Pro Asp Gly Trp

55 60 65

gag cag atc gtt cca gat cct gta ccg gag att cag gcg atg gtt ccc 355

Glu Gln Ile Val Pro Asp Pro Val Pro Glu Ile Gln Ala Met Val Pro

70 75 80 85

gaa gct ctg gct cag cgc ggt gtg ctc acc gcc ggt gcc aac cca cct 403

Glu Ala Leu Ala Gln Arg Gly Val Leu Thr Ala Gly Ala Asn Pro Pro

90 95 100

ttc cca ccg ttt gag ttt aaa gat tcc gat ggt cag att atc ggt gtg 451

Phe Pro Pro Phe Glu Phe Lys Asp Ser Asp Gly Gln Ile Ile Gly Val

105 110 115

gaa atg gac ctc gtg cgt gca atg gcg ggg gtg atg ggc ttg gag ttc 499

Glu Met Asp Leu Val Arg Ala Met Ala Gly Val Met Gly Leu Glu Phe

120 125 130

agc cct cag gag cag gat ttc tcc ctc atc ctt cca tcg gtt caa gct 547

Ser Pro Gln Glu Gln Asp Phe Ser Leu Ile Leu Pro Ser Val Gln Ala

135 140 145

ggc acc ctt gat atc gga gcc tct ggc ttc act gac aac gag gaa cgc 595

Gly Thr Leu Asp Ile Gly Ala Ser Gly Phe Thr Asp Asn Glu Glu Arg

150 155 160 165

cgc gag aac ttt gat ttc atc gat ttc ctc ttc gca ggt gtg cag tgg 643

Arg Glu Asn Phe Asp Phe Ile Asp Phe Leu Phe Ala Gly Val Gln Trp

170 175 180

gcg cag gca act gat cgt gaa acc cca atc gat ccg gaa aac gcc tgt 691

Ala Gln Ala Thr Asp Arg Glu Thr Pro Ile Asp Pro Glu Asn Ala Cys

185 190 195

ggt ctc acc gtt gct gta cag cgc aca acc gtg gca gag acc gac gat 739

Gly Leu Thr Val Ala Val Gln Arg Thr Thr Val Ala Glu Thr Asp Asp

200 205 210

gtc cgt cct cgc tca gct caa tgt gaa gcc gaa ggc aaa gag ccg atc 787

Val Arg Pro Arg Ser Ala Gln Cys Glu Ala Glu Gly Lys Glu Pro Ile

215 220 225

acc att ttg tct tat gaa acc gca gat act gca gct acc gca ttg atc 835

Thr Ile Leu Ser Tyr Glu Thr Ala Asp Thr Ala Ala Thr Ala Leu Ile

230 235 240 245

ctg gga cgc gca gac gca ctt gct gcg gac tcc cct gtt tca gct tgg 883

Leu Gly Arg Ala Asp Ala Leu Ala Ala Asp Ser Pro Val Ser Ala Trp

250 255 260

gct gca gag cgc tcc gaa ggc cgc atc gaa gtt gtg ggc gat atg tac 931

Ala Ala Glu Arg Ser Glu Gly Arg Ile Glu Val Val Gly Asp Met Tyr

265 270 275

ttg gct gcg cca ttt ggt ttc gca ttc ccg ttg gaa tct gac ctc acc 979

Leu Ala Ala Pro Phe Gly Phe Ala Phe Pro Leu Glu Ser Asp Leu Thr

280 285 290

cca gca gca gct gcg gcg ttc caa cac ttg att gac acc ggc gat tac 1027

Pro Ala Ala Ala Ala Ala Phe Gln His Leu Ile Asp Thr Gly Asp Tyr

295 300 305

cag cgc atc atg gcg caa tgg ggc att gaa gaa ggc ctt ctt gat gag 1075

Gln Arg Ile Met Ala Gln Trp Gly Ile Glu Glu Gly Leu Leu Asp Glu

310 315 320 325

gcc ctg atc aac gaa cag cca ctc aac tagagccttc cagcaactaa 1122

Ala Leu Ile Asn Glu Gln Pro Leu Asn

330

aaacaattaa 1132

<210>34

<211>334

<212>PRT

<213>谷氨酸棒状杆菌

<400>34

Met Ala Gly Ser Ile Leu Pro Met Ile Glu Ser Phe Thr Arg Leu Arg

1 5 10 15

Ile Ser Gln Thr Pro Pro Leu Lys Arg Leu Leu His Ser Thr Leu Arg

20 25 30

Phe Pro Lys Ile Pro Lys Arg Ala Val Ala Ala Thr Val Gly Ile Val

35 40 45

Ala Thr Ser Phe Thr Leu Ala Ser Cys Val Thr Asn Glu Glu Gln Gly

50 55 60

Asn Pro Asp Gly Trp Glu Gln Ile Val Pro Asp Pro Val Pro Glu Ile

65 70 75 80

Gln Ala Met Val Pro Glu Ala Leu Ala Gln Arg Gly Val Leu Thr Ala

85 90 95

Gly Ala Asn Pro Pro Phe Pro Pro Phe Glu Phe Lys Asp Ser Asp Gly

100 105 110

Gln Ile Ile Gly Val Glu Met Asp Leu Val Arg Ala Met Ala Gly Val

115 120 125

Met Gly Leu Glu Phe Ser Pro Gln Glu Gln Asp Phe Ser Leu Ile Leu

130 135 140

Pro Ser Val Gln Ala Gly Thr Leu Asp Ile Gly Ala Ser Gly Phe Thr

145 150 155 160

Asp Asn Glu Glu Arg Arg Glu Asn Phe Asp Phe Ile Asp Phe Leu Phe

165 170 175

Ala Gly Val Gln Trp Ala Gln Ala Thr Asp Arg Glu Thr Pro Ile Asp

180 185 190

Pro Glu Asn Ala Cys Gly Leu Thr Val Ala Val Gln Arg Thr Thr Val

195 200 205

Ala Glu Thr Asp Asp Val Arg Pro Arg Ser Ala Gln Cys Glu Ala Glu

210 215 220

Gly Lys Glu Pro Ile Thr Ile Leu Ser Tyr Glu Thr Ala Asp Thr Ala

225 230 235 240

Ala Thr Ala Leu Ile Leu Gly Arg Ala Asp Ala Leu Ala Ala Asp Ser

245 250 255

Pro Val Ser Ala Trp Ala Ala Glu Arg Ser Glu Gly Arg Ile Glu Val

260 265 270

Val Gly Asp Met Tyr Leu Ala Ala Pro Phe Gly Phe Ala Phe Pro Leu

275 280 285

Glu Ser Asp Leu Thr Pro Ala Ala Ala Ala Ala Phe Gln His Leu Ile

290 295 300

Asp Thr Gly Asp Tyr Gln Arg Ile Met Ala Gln Trp Gly Ile Glu Glu

305 310 315 320

Gly Leu Leu Asp Glu Ala Leu Ile Asn Glu Gln Pro Leu Asn

325 330

<210>35

<211>2389

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2359)

<223>RXA00867

<400>35

agagaactgg taaggttttt accgttctag accgcagaaa tcttcgcggc gacaccgatg 60

atcgccgagc agaactaaac atgaggagac ctactcgcat atg agc gat gta aag 115

Met Ser Asp Val Lys

1 5

tac ttc gaa gac acc gaa ttt ggc ctg atc gag gcc gtc gca acc atc 163

Tyr Phe Glu Asp Thr Glu Phe Gly Leu Ile Glu Ala Val Ala Thr Ile

10 15 20

gac aac ggt gac ttc gga acc cgc acc atc cgt ttt gaa acc ggc caa 21l

Asp Asn Gly Asp Phe Gly Thr Arg Thr Ile Arg Phe Glu Thr Gly Gln

25 30 35

ctt gcc cgc cag gca gat ggt gca gtg acc acc tac ctc gac gat gac 259

Leu Ala Arg Gln Ala Asp Gly Ala Val Thr Thr Tyr Leu Asp Asp Asp

40 45 50

acg atg ctg ctg gca acc acc acc gca tcc aac cag cca cgc gag ggc 307

Thr Met Leu Leu Ala Thr Thr Thr Ala Ser Asn Gln Pro Arg Glu Gly

55 60 65

ttt gac ttc ttc cca ctg acc gtg gac gtt gaa gag cgt atg tac gca 355

Phe Asp Phe Phe Pro Leu Thr Val Asp Val Glu Glu Arg Met Tyr Ala

70 75 80 85

gct ggt cgc atc cct ggc tct ttc ttc cgt cgg gag ggt cgc cca tcc 403

Ala Gly Arg Ile Pro Gly Ser Phe Phe Arg Arg Glu Gly Arg Pro Ser

90 95 100

acc gaa gct atc ctg gct tgc cgt ctc atc gac cgc cca ctg cgc cca 451

Thr Glu Ala Ile Leu Ala Cys Arg Leu Ile Asp Arg Pro Leu Arg Pro

105 110 115

acc ttt gtt aag ggc ctg cgc aat gag gtt cag atc gtt gtc acc gtc 499

Thr Phe Val Lys Gly Leu Arg Asn Glu Val Gln Ile Val Val Thr Val

120 125 130

atg tcc atg aac cct gag gat tac tac gat gtc gta gca atc aac gga 547

Met Ser Met Asn Pro Glu Asp Tyr Tyr Asp Val Val Ala Ile Asn Gly

135 140 145

gct tcc gca gca acc cgc atc tcc gga ctt cct gtc tcc ggc gct gtc 595

Ala Ser Ala Ala Thr Arg Ile Ser Gly Leu Pro Val Ser Gly Ala Val

150 155 160 165

ggt ggc gtt cgc atg gca ctg gtt gtt gat gaa aag cac cca gaa ggc 643

Gly Gly Val Arg Met Ala Leu Val Val Asp Glu Lys His Pro Glu Gly

170 175 180

caa tgg gtt gca ttc cca acc cac gct caa cat gag cag tcc gta ttt 691

Gln Trp Val Ala Phe Pro Thr His Ala Gln His Glu Gln Ser Val Phe

185 190 195

gaa atc gtt gtg gct ggt cgc ctc gtc gag cgc aag cgc ggc aac aag 739

Glu Ile Val Val Ala Gly Arg Leu Val Glu Arg Lys Arg Gly Asn Lys

200 205 210

acc ttc tcc gac gtc gca gtg atg atg gtg gaa gct ggc gct tcc gaa 787

Thr Phe Ser Asp Val Ala Val Met Met Val Glu Ala Gly Ala Ser Glu

215 220 225

aac gtt gtc aac cgc gtc aag gac ggt gca cca gca cca acc gaa aag 835

Asn Val Val Asn Arg Val Lys Asp Gly Ala Pro Ala Pro Thr Glu Lys

230 235 240 245

atc gtc tcc gac ggc ctt gaa gca gct aag cca ttc atc gac atc ctg 883

Ile Val Ser Asp Gly Leu Glu Ala Ala Lys Pro Phe Ile Asp Ile Leu

250 255 260

tgc cgc gca cag gaa ggt ctg gca cag cgc gtt gga aac gca gcc aag 931

Cys Arg Ala Gln Glu Gly Leu Ala Gln Arg Val Gly Asn Ala Ala Lys

265 270 275

gaa ttc cca ctg ttc cct cca tac acc gac gag gtg tac tcc gca gtg 979

Glu Phe Pro Leu Phe Pro Pro Tyr Thr Asp Glu Val Tyr Ser Ala Val

280 285 290

gag cgc aag gta tcc aag aag cta gct tct ttg ctg acc ctg aag gca 1027

Glu Arg Lys Val Ser Lys Lys Leu Ala Ser Leu Leu Thr Leu Lys Ala

295 300 305

aag caa gag cgc gac gac gct acc aac gcc tac atg gaa gaa atc gaa 1075

Lys Gln Glu Arg Asp Asp Ala Thr Asn Ala Tyr Met Glu Glu Ile Glu

310 315 320 325

gcc gaa ctg ctt cca aag ttc gag gct tcc tac agc tca gca gct gaa 1123

Ala Glu Leu Leu Pro Lys Phe Glu Ala Ser Tyr Ser Ser Ala Ala Glu

330 335 340

gcg tcc aag gaa atc cgt gca gga tac aac gct gtc atg aag gcc atc 1171

Ala Ser Lys Glu Ile Arg Ala Gly Tyr Asn Ala Val Met Lys Ala Ile

345 350 355

gtg cgc cgc atg atc ctc acc gat cac ttc cgc atc gac ggc cgc gga 1219

Val Arg Arg Met Ile Leu Thr Asp His Phe Arg Ile Asp Gly Arg Gly

360 365 370

gtc acc gac atc cgt gac ctg gca gta gaa gtt gag ctc atc cca cgt 1267

Val Thr Asp Ile Arg Asp Leu Ala Val Glu Val Glu Leu Ile Pro Arg

375 380 385

gcg cac ggt tcc tcc ctc ttc gag cgt ggc gag acc cag atc ctt ggt 1315

Ala His Gly Ser Ser Leu Phe Glu Arg Gly Glu Thr Gln Ile Leu Gly

390 395 400 405

gtc acc acc ctg gac atg ctc aag atg gaa cag caa atc gac tcc ctg 1363

Val Thr Thr Leu Asp Met Leu Lys Met Glu Gln Gln Ile Asp Ser Leu

410 415 420

gca cca ggc gat gcg aag cgc tac atg cac cac tac aac ttc cct cca 1411

Ala Pro Gly Asp Ala Lys Arg Tyr Met His His Tyr Asn Phe Pro Pro

425 430 435

tac tcc acc ggt gaa acc ggt cgt gtg ggc tca cca aag cgc cgc gaa 1459

Tyr Ser Thr Gly Glu Thr Gly Arg Val Gly Ser Pro Lys Arg Arg Glu

440 445 450

atc ggc cac ggt gca ctt gca gaa cgc gca gtt ttg cca gta atc cca 1507

Ile Gly His Gly Ala Leu Ala Glu Arg Ala Val Leu Pro Val Ile Pro

455 460 465

tcc cgt gag gaa ttc cca tac gca atc cgt cag gtc tct gaa gct ctg 1555

Ser Arg Glu Glu Phe Pro Tyr Ala Ile Arg Gln Val Ser Glu Ala Leu

470 475 480 485

ggc tcc aac ggc tcc acc tcc atg ggc tct gtc tgt gca tcc act ctg 1603

Gly Ser Asn Gly Ser Thr Ser Met Gly Ser Val Cys Ala Ser Thr Leu

490 495 500

tcc ctg tac aac gct ggt gtt cca ctg aag gca cct gtt gca ggt atc 1651

Ser Leu Tyr Asn Ala Gly Val Pro Leu Lys Ala Pro Val Ala Gly Ile

505 510 515

gcc atg gga ctt gtt tcc ggt gaa atc gac ggc aag acc gag tac gtt 1699

Ala Met Gly Leu Val Ser Gly Glu Ile Asp Gly Lys Thr Glu Tyr Val

520 525 530

gca ctg acc gac atc ctc ggc gca gaa gac gca ttc ggc gac atg gac 1747

Ala Leu Thr Asp Ile Leu Gly Ala Glu Asp Ala Phe Gly Asp Met Asp

535 540 545

ttc aag gtt gcc ggc acc gca gac ttc atc acc gca ctt cag ctg gac 1795

Phe Lys Val Ala Gly Thr Ala Asp Phe Ile Thr Ala Leu Gln Leu Asp

550 555 560 565

acc aag ctg gac ggc att cct tcc aag gtg ctc tcc gat gcg ctt gag 1843

Thr Lys Leu Asp Gly Ile Pro Ser Lys Val Leu Ser Asp Ala Leu Glu

570 575 580

cag gca cgc gat gcc cga ctg acc atc ctg aac acc atg gct gat gtc 1891

Gln Ala Arg Asp Ala Arg Leu Thr Ile Leu Asn Thr Met Ala Asp Val

585 590 595

atc aac gga cct gat gag atg agc aag ttc gct cct cgc atc acc acc 1939

Ile Asn Gly Pro Asp Glu Met Ser Lys Phe Ala Pro Arg Ile Thr Thr

600 605 610

gtg aag atc cca gtg gca aag atc ggt gag ctg atc gga cca aag ggt 1987

Val Lys Ile Pro Val Ala Lys Ile Gly Glu Leu Ile Gly Pro Lys Gly

615 620 625

aag aac atc aac gct ctt acc gaa gag acc ggc gca aac atc tcc atc 2035

Lys Asn Ile Asn Ala Leu Thr Glu Glu Thr Gly Ala Asn Ile Ser Ile

630 635 640 645

gaa gat gac ggc acc gtg ttc atc tct gca gct gac ggc gca tct gct 2083

Glu Asp Asp Gly Thr Val Phe Ile Ser Ala Ala Asp Gly Ala Ser Ala

650 655 660

gaa gcg gcg atc gaa aag atc aac gct ctg gcg aac cca cag ctg cca 2131

Glu Ala Ala Ile Glu Lys Ile Asn Ala Leu Ala Asn Pro Gln Leu Pro

665 670 675

aag gtt ggc gag cgc ttc ctc gga acc gtc gtc aag acc acc gca ttc 2179

Lys Val Gly Glu Arg Phe Leu Gly Thr Val Val Lys Thr Thr Ala Phe

680 685 690

gga gca ttc gtt tcc ttg ctc cca ggc cgc gac ggc ctt gtt cac atc 2227

Gly Ala Phe Val Ser Leu Leu Pro Gly Arg Asp Gly Leu Val His Ile

695 700 705

tcc aag ctg ggt aac ggc aag cga gta gaa aag gtc gac gat gtg gtg 2275

Ser Lys Leu Gly Asn Gly Lys Arg Val Glu Lys Val Asp Asp Val Val

710 715 720 725

aag gtt ggc gag aag att cag gtc gaa atc gct gac atc gac aac cgc 2323

Lys Val Gly Glu Lys Ile Gln Val Glu Ile Ala Asp Ile Asp Asn Arg

730 735 740

ggc aag atc tcc ttg gtc cca gtt gtt gaa gag gac taattagttc 2369

Gly Lys Ile Ser Leu Val Pro Val Val Glu Glu Asp

745 750

tggctagatc gggctagatc 2389

<210>36

<211>753

<212>PRT

<213>谷氨酸棒状杆菌

<400>36

Met Ser Asp Val Lys Tyr Phe Glu Asp Thr Glu Phe Gly Leu Ile Glu

1 5 10 15

Ala Val Ala Thr Ile Asp Asn Gly Asp Phe Gly Thr Arg Thr Ile Arg

20 25 30

Phe Glu Thr Gly Gln Leu Ala Arg Gln Ala Asp Gly Ala Val Thr Thr

35 40 45

Tyr Leu Asp Asp Asp Thr Met Leu Leu Ala Thr Thr Thr Ala Ser Asn

50 55 60

Gln Pro Arg Glu Gly Phe Asp Phe Phe Pro Leu Thr Val Asp Val Glu

65 70 75 80

Glu Arg Met Tyr Ala Ala Gly Arg Ile Pro Gly Ser Phe Phe Arg Arg

85 90 95

Glu Gly Arg Pro Ser Thr Glu Ala Ile Leu Ala Cys Arg Leu Ile Asp

100 105 110

Arg Pro Leu Arg Pro Thr Phe Val Lys Gly Leu Arg Asn Glu Val Gln

115 120 125

Ile Val Val Thr Val Met Ser Met Asn Pro Glu Asp Tyr Tyr Asp Val

130 135 140

Val Ala Ile Asn Gly Ala Ser Ala Ala Thr Arg Ile Ser Gly Leu Pro

145 150 155 160

Val Ser Gly Ala Val Gly Gly Val Arg Met Ala Leu Val Val Asp Glu

165 170 175

Lys His Pro Glu Gly Gln Trp Val Ala Phe Pro Thr His Ala Gln His

180 185 190

Glu Gln Ser Val Phe Glu Ile Val Val Ala Gly Arg Leu Val Glu Arg

195 200 205

Lys Arg Gly Asn Lys Thr Phe Ser Asp Val Ala Val Met Met Val Glu

210 215 220

Ala Gly Ala Ser Glu Asn Val Val Asn Arg Val Lys Asp Gly Ala Pro

225 230 235 240

Ala Pro Thr Glu Lys Ile Val Ser Asp Gly Leu Glu Ala Ala Lys Pro

245 250 255

Phe Ile Asp Ile Leu Cys Arg Ala Gln Glu Gly Leu Ala Gln Arg Val

260 265 270

Gly Asn Ala Ala Lys Glu Phe Pro Leu Phe Pro Pro Tyr Thr Asp Glu

275 280 285

Val Tyr Ser Ala Val Glu Arg Lys Val Ser Lys Lys Leu Ala Ser Leu

290 295 300

Leu Thr Leu Lys Ala Lys Gln Glu Arg Asp Asp Ala Thr Asn Ala Tyr

305 310 315 320

Met Glu Glu Ile Glu Ala Glu Leu Leu Pro Lys Phe Glu Ala Ser Tyr

325 330 335

Ser Ser Ala Ala Glu Ala Ser Lys Glu Ile Arg Ala Gly Tyr Asn Ala

340 345 350

Val Met Lys Ala Ile Val Arg Arg Met Ile Leu Thr Asp His Phe Arg

355 360 365

Ile Asp Gly Arg Gly Val Thr Asp Ile Arg Asp Leu Ala Val Glu Val

370 375 380

Glu Leu Ile Pro Arg Ala His Gly Ser Ser Leu Phe Glu Arg Gly Glu

385 390 395 400

Thr Gln Ile Leu Gly Val Thr Thr Leu Asp Met Leu Lys Met Glu Gln

405 410 415

Gln Ile Asp Ser Leu Ala Pro Gly Asp Ala Lys Arg Tyr Met His His

420 425 430

Tyr Asn Phe Pro Pro Tyr Ser Thr Gly Glu Thr Gly Arg Val Gly Ser

435 440 445

Pro Lys Arg Arg Glu Ile Gly His Gly Ala Leu Ala Glu Arg Ala Val

450 455 460

Leu Pro Val Ile Pro Ser Arg Glu Glu Phe Pro Tyr Ala Ile Arg Gln

465 470 475 480

Val Ser Glu Ala Leu Gly Ser Asn Gly Ser Thr Ser Met Gly Ser Val

485 490 495

Cys Ala Ser Thr Leu Ser Leu Tyr Asn Ala Gly Val Pro Leu Lys Ala

500 505 510

Pro Val Ala Gly Ile Ala Met Gly Leu Val Ser Gly Glu Ile Asp Gly

515 520 525

Lys Thr Glu Tyr Val Ala Leu Thr Asp Ile Leu Gly Ala Glu Asp Ala

530 535 540

Phe Gly Asp Met Asp Phe Lys Val Ala Gly Thr Ala Asp Phe Ile Thr

545 550 555 560

Ala Leu Gln Leu Asp Thr Lys Leu Asp Gly Ile Pro Ser Lys Val Leu

565 570 575

Ser Asp Ala Leu Glu Gln Ala Arg Asp Ala Arg Leu Thr Ile Leu Asn

580 585 590

Thr Met Ala Asp Val Ile Asn Gly Pro Asp Glu Met Ser Lys Phe Ala

595 600 605

Pro Arg Ile Thr Thr Val Lys Ile Pro Val Ala Lys Ile Gly Glu Leu

610 615 620

Ile Gly Pro Lys Gly Lys Asn Ile Asn Ala Leu Thr Glu Glu Thr Gly

625 630 635 640

Ala Asn Ile Ser Ile Glu Asp Asp Gly Thr Val Phe Ile Ser Ala Ala

645 650 655

Asp Gly Ala Ser Ala Glu Ala Ala Ile Glu Lys Ile Asn Ala Leu Ala

660 665 670

Asn Pro Gln Leu Pro Lys Val Gly Glu Arg Phe Leu Gly Thr Val Val

675 680 685

Lys Thr Thr Ala Phe Gly Ala Phe Val Ser Leu Leu Pro Gly Arg Asp

690 695 700

Gly Leu Val His Ile Ser Lys Leu Gly Asn Gly Lys Arg Val Glu Lys

705 710 715 720

Val Asp Asp Val Val Lys Val Gly Glu Lys Ile Gln Val Glu Ile Ala

725 730 735

Asp Ile Asp Asn Arg Gly Lys Ile Ser Leu Val Pro Val Val Glu Glu

740 745 750

Asp

<210>37

<211>1624

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1594)

<223>RXA00955

<400>37

gcacaccgtg atgcgttggc tgcgtccgca ggtgcgatgt tctacctcaa cggcgatgtc 60

gactccttga aagatggtgc acaaaaggcg ctttccttgc ttg ccg acg gca cca 115

Leu Pro Thr Ala Pro

1 5

ccc agg cat ggt tgg cca agc acg aag aga tcg att act cag aaa agg 163

Pro Arg His Gly Trp Pro Ser Thr Lys Arg Ser Ile Thr Gln Lys Arg

10 15 20

agt ctt cca atg act agt aat aat ctg ccc aca gtg ttg gaa agc atc 211

Ser Leu Pro Met Thr Ser Asn Asn Leu Pro Thr Val Leu Glu Ser Ile

25 30 35

gtc gag ggt cgt cgc gga cac ctg gag gaa att cgc gct cgc atc gct 259

Val Glu Gly Arg Arg Gly His Leu Glu Glu Ile Arg Ala Arg Ile Ala

40 45 50

cac gtg gat gtg gat gcg ctt cca aaa tcc acc cgt tct ctg ttt gat 307

His Val Asp Val Asp Ala Leu Pro Lys Ser Thr Arg Ser Leu Phe Asp

55 60 65

tcc ctc aac cag ggt agg gga ggg gcg cgt ttc atc atg gag tgc aag 355

Ser Leu Asn Gln Gly Arg Gly Gly Ala Arg Phe Ile Met Glu Cys Lys

70 75 80 85

tcc gca tcg cct tct ttg gga atg att cgt gag cac tac cag ccg ggt 403

Ser Ala Ser Pro Ser Leu Gly Met Ile Arg Glu His Tyr Gln Pro Gly

90 95 100

gaa atc gct cgc gtg tac tct cgc tac gcc agc ggc att tcc gtg ctg 451

Glu Ile Ala Arg Val Tyr Ser Arg Tyr Ala Ser Gly Ile Ser Val Leu

105 110 115

tgc gag ccg gat cgt ttt ggt ggc gat tac gat cac ctc gct acc gtt 499

Cys Glu Pro Asp Arg Phe Gly Gly Asp Tyr Asp His Leu Ala Thr Val

120 125 130

gcc gct acc tct cat ctt ccg gtg ctg tgc aaa gac ttc atc att gat 547

Ala Ala Thr Ser His Leu Pro Val Leu Cys Lys Asp Phe Ile Ile Asp

135 140 145

cct gtc cag gta cac gcg gcg cgt tac ttt ggt gct gat gcc atc ctg 595

Pro Val Gln Val His Ala Ala Arg Tyr Phe Gly Ala Asp Ala Ile Leu

150 155 160 165

ctc atg ctc tct gtg ctt gat gat gaa gag tac gca gca ctc gct gcc 643

Leu Met Leu Ser Val Leu Asp Asp Glu Glu Tyr Ala Ala Leu Ala Ala

170 175 180

gag gct gcg cgt ttt gat ctg gat atc ctc acc gag gtt att gat gag 691

Glu Ala Ala Arg Phe Asp Leu Asp Ile Leu Thr Glu Val Ile Asp Glu

185 190 195

gag gaa gtc gcc cgc gcc atc aag ctg ggt gcg aag atc ttt ggc gtc 739

Glu Glu Val Ala Arg Ala Ile Lys Leu Gly Ala Lys Ile Phe Gly Val

200 205 210

aac cac cgc aac ctg cat gat ctg tcc att gat ttg gat cgt tca cgt 787

Asn His Arg Asn Leu His Asp Leu Ser Ile Asp Leu Asp Arg Ser Arg

215 220 225

cgc ctg tcc aag ctc att cca gca gat gcc gtg ctc gtg tct gag tct 835

Arg Leu Ser Lys Leu Ile Pro Ala Asp Ala Val Leu Val Ser Glu Ser

230 235 240 245

ggc gtg cgc gat acc gaa acc gtc cgc cag cta ggt ggg cac tcc aat 883

Gly Val Arg Asp Thr Glu Thr Val Arg Gln Leu Gly Gly His Ser Asn

250 255 260

gca ttc ctc gtt ggc tcc cag ctg acc agc cag gaa aac gtc gat ctg 931

Ala Phe Leu Val Gly Ser Gln Leu Thr Ser Gln Glu Asn Val Asp Leu

265 270 275

gca gcc cgc gaa tta gtc tac ggc ccc aac aaa gtc tgc gga ctc acc 979

Ala Ala Arg Glu Leu Val Tyr Gly Pro Asn Lys Val Cys Gly Leu Thr

280 285 290

tca cca agt gca gca caa acc gct cgc gca gcg ggt gcg gtc tac ggc 1027

Ser Pro Ser Ala Ala Gln Thr Ala Arg Ala Ala Gly Ala Val Tyr Gly

295 300 305

ggg ctc atc ttc gaa gag gca tcg cca cgc aat gtt tca cgt gaa aca 1075

Gly Leu Ile Phe Glu Glu Ala Ser Pro Arg Asn Val Ser Arg Glu Thr

310 315 320 325

ttg caa aaa atc atc gcc gca gag ccc aac ctg cgc tac gtc gcg gtc 1123

Leu Gln Lys Ile Ile Ala Ala Glu Pro Asn Leu Arg Tyr Val Ala Val

330 335 340

agc cgt cgc acc tcc ggg tac aag gat ttg ctt gtc gac ggc atc ttc 1171

Ser Arg Arg Thr Ser Gly Tyr Lys Asp Leu Leu Val Asp Gly Ile Phe

345 350 355

gcc gta caa atc cac gcc cca ctg cag gac agc gtc gaa gca gaa aag 1219

Ala Val Gln Ile His Ala Pro Leu Gln Asp Ser Val Glu Ala Glu Lys

360 365 370

gca ttg atc gcc gcc gtt cgt gaa gag gtt gga ccg cag gtc cag gtc 1267

Ala Leu Ile Ala Ala Val Arg Glu Glu Val Gly Pro Gln Val Gln Val

375 380 385

tgg cgc gcg atc tcg atg tcc agc ccc ttg ggg gct gaa gtg gca gct 1315

Trp Arg Ala Ile Ser Met Ser Ser Pro Leu Gly Ala Glu Val Ala Ala

390 395 400 405

gcg gtg gag ggt gac gtc gat aag cta att ctt gat gcc cat gaa ggt 1363

Ala Val Glu Gly Asp Val Asp Lys Leu Ile Leu Asp Ala His Glu Gly

410 415 420

ggc agc ggg gaa gta ttc gac tgg gct acg gtg ccg gcc gct gtg aag 1411

Gly Ser Gly Glu Val Phe Asp Trp Ala Thr Val Pro Ala Ala Val Lys

425 430 435

gca aag tct ttg ctc gcg gga ggc atc tct ccg gac aac gct gcg cag 1459

Ala Lys Ser Leu Leu Ala Gly Gly Ile Ser Pro Asp Asn Ala Ala Gln

440 445 450

gca ctc gct gtg ggc tgc gca ggt ttg gac atc aac tct ggc gtg gaa 1507

Ala Leu Ala Val Gly Cys Ala Gly Leu Asp Ile Asn Ser Gly Val Glu

455 460 465

tac ccc gcc ggt gca ggc acg tgg gct ggg gcg aaa gac gcc ggc gcg 1555

Tyr Pro Ala Gly Ala Gly Thr Trp Ala Gly Ala Lys Asp Ala Gly Ala

470 475 480 485

ctg ctg aaa att tta gcg acc atc tcc aca ttc cat tac taaaggttta 1604

Leu Leu Lys Ile Leu Ala Thr Ile Ser Thr Phe His Tyr

490 495

aataggatca tgactgaaaa 1624

<210>38

<211>498

<212>PRT

<213>谷氨酸棒状杆菌

<400>38

Leu Pro Thr Ala Pro Pro Arg His Gly Trp Pro Ser Thr Lys Arg Ser

1 5 10 15

Ile Thr Gln Lys Arg Ser Leu Pro Met Thr Ser Asn Asn Leu Pro Thr

20 25 30

Val Leu Glu Ser Ile Val Glu Gly Arg Arg Gly His Leu Glu Glu Ile

35 40 45

Arg Ala Arg Ile Ala His Val Asp Val Asp Ala Leu Pro Lys Ser Thr

50 55 60

Arg Ser Leu Phe Asp Ser Leu Asn Gln Gly Arg Gly Gly Ala Arg Phe

65 70 75 80

Ile Met Glu Cys Lys Ser Ala Ser Pro Ser Leu Gly Met Ile Arg Glu

85 90 95

His Tyr Gln Pro Gly Glu Ile Ala Arg Val Tyr Ser Arg Tyr Ala Ser

100 105 110

Gly Ile Ser Val Leu Cys Glu Pro Asp Arg Phe Gly Gly Asp Tyr Asp

115 120 125

His Leu Ala Thr Val Ala Ala Thr Ser His Leu Pro Val Leu Cys Lys

130 135 140

Asp Phe Ile Ile Asp Pro Val Gln Val His Ala Ala Arg Tyr Phe Gly

145 150 155 160

Ala Asp Ala Ile Leu Leu Met Leu Ser Val Leu Asp Asp Glu Glu Tyr

165 170 175

Ala Ala Leu Ala Ala Glu Ala Ala Arg Phe Asp Leu Asp Ile Leu Thr

180 185 190

Glu Val Ile Asp Glu Glu Glu Val Ala Arg Ala Ile Lys Leu Gly Ala

195 200 205

Lys Ile Phe Gly Val Asn His Arg Asn Leu His Asp Leu Ser Ile Asp

210 215 220

Leu Asp Arg Ser Arg Arg Leu Ser Lys Leu Ile Pro Ala Asp Ala Val

225 230 235 240

Leu Val Ser Glu Ser Gly Val Arg Asp Thr Glu Thr Val Arg Gln Leu

245 250 255

Gly Gly His Ser Asn Ala Phe Leu Val Gly Ser Gln Leu Thr Ser Gln

260 265 270

Glu Asn Val Asp Leu Ala Ala Arg Glu Leu Val Tyr Gly Pro Asn Lys

275 280 285

Val Cys Gly Leu Thr Ser Pro Ser Ala Ala Gln Thr Ala Arg Ala Ala

290 295 300

Gly Ala Val Tyr Gly Gly Leu Ile Phe Glu Glu Ala Ser Pro Arg Asn

305 310 315 320

Val Ser Arg Glu Thr Leu Gln Lys Ile Ile Ala Ala Glu Pro Asn Leu

325 330 335

Arg Tyr Val Ala Val Ser Arg Arg Thr Ser Gly Tyr Lys Asp Leu Leu

340 345 350

Val Asp Gly Ile Phe Ala Val Gln Ile His Ala Pro Leu Gln Asp Ser

355 360 365

Val Glu Ala Glu Lys Ala Leu Ile Ala Ala Val Arg Glu Glu Val Gly

370 375 380

Pro Gln Val Gln Val Trp Arg Ala Ile Ser Met Ser Ser Pro Leu Gly

385 390 395 400

Ala Glu Val Ala Ala Ala Val Glu Gly Asp Val Asp Lys Leu Ile Leu

405 410 415

Asp Ala His Glu Gly Gly Ser Gly Glu Val Phe Asp Trp Ala Thr Val

420 425 430

Pro Ala Ala Val Lys Ala Lys Ser Leu Leu Ala Gly Gly Ile Ser Pro

435 440 445

Asp Asn Ala Ala Gln Ala Leu Ala Val Gly Cys Ala Gly Leu Asp Ile

450 455 460

Asn Ser Gly Val Glu Tyr Pro Ala Gly Ala Gly Thr Trp Ala Gly Ala

465 470 475 480

Lys Asp Ala Gly Ala Leu Leu Lys Ile Leu Ala Thr Ile Ser Thr Phe

485 490 495

His Tyr

<210>39

<211>1684

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1654)

<223>RXA00957

<400>39

aaccggcaag ccctggatcg aatgaagctc gcagcgagta attatttgat gtttcccaga 60

aaggcttcag ccccacaatg atttcctcgg taggtgcccc atg agc acg aat ccc 115

Met Ser Thr Asn Pro

1 5

cat gtt ttc tcc cta gat gtc cgc tat cac gag gat gct tct gca ttg 163

His Val Phe Ser Leu Asp Val Arg Tyr His Glu Asp Ala Ser Ala Leu

10 15 20

ttt gcc cac ttg ggt ggc aca acc gca gat gat gca gcc ctg ttg gaa 211

Phe Ala His Leu Gly Gly Thr Thr Ala Asp Asp Ala Ala Leu Leu Glu

25 30 35

agc gct gat atc acc acc aag aat ggt att tct tcc ctc gcg gtg ttg 259

Ser Ala Asp Ile Thr Thr Lys Asn Gly Ile Ser Ser Leu Ala Val Leu

40 45 50

aag agt tcg gtg cgc att acg tgc acg ggc aac acg gtg gta acg cag 307

Lys Ser Ser Val Arg Ile Thr Cys Thr Gly Asn Thr Val Val Thr Gln

55 60 65

ccg ctg acg gac tcg ggt agg gca gtg gtt gcg cgc cta acg cag cag 355

Pro Leu Thr Asp Ser Gly Arg Ala Val Val Ala Arg Leu Thr Gln Gln

70 75 80 85

ctt ggc cag tac aac acc gca gag aac acc ttt agc ttc ccc gcc tca 403

Leu Gly Gln Tyr Asn Thr Ala Glu Asn Thr Phe Ser Phe Pro Ala Ser

90 95 100

gat gcg gtt gat gag cgc gag cgc ctc acc gca cca agc acc atc gaa 451

Asp Ala Val Asp Glu Arg Glu Arg Leu Thr Ala Pro Ser Thr Ile Glu

105 110 115

gtg ctg cgc aag ttg cag ttc gag tcc ggc tac agc gac gcg tcc ctg 499

Val Leu Arg Lys Leu Gln Phe Glu Ser Gly Tyr Ser Asp Ala Ser Leu

120 125 130

cca ctg ctc atg ggc ggt ttc gcg ttt gat ttc tta gaa acc ttt gaa 547

Pro Leu Leu Met Gly Gly Phe Ala Phe Asp Phe Leu Glu Thr Phe Glu

135 140 145

acg ctc ccc gct gtc gag gag agc gtc aac act tac ccc gat tac cag 595

Thr Leu Pro Ala Val Glu Glu Ser Val Asn Thr Tyr Pro Asp Tyr Gln

150 155 160 165

ttc gtc ctc gcg gaa atc gtc ctg gac atc aat cac cag gac cag acc 643

Phe Val Leu Ala Glu Ile Val Leu Asp Ile Asn His Gln Asp Gln Thr

170 175 180

gcc aaa ctc gcc ggc gtc tcc aac gcc cca ggc gag ctc gag gcc gag 691

Ala Lys Leu Ala Gly Val Ser Asn Ala Pro Gly Glu Leu Glu Ala Glu

185 190 195

ctc aac aag ctt tca ttg ctt atc gac gcc gcc ctc ccc gca acc gaa 739

Leu Asn Lys Leu Ser Leu Leu Ile Asp Ala Ala Leu Pro Ala Thr Glu

200 205 210

cac gcc tac caa acc acc cct cac gac ggc gac act ctt cgc gtt gtg 787

His Ala Tyr Gln Thr Thr Pro His Asp Gly Asp Thr Leu Arg Val Val

215 220 225

gct gat att ccc gat gct cag ttc cgc acc cag atc aat gag ctg aaa 835

Ala Asp Ile Pro Asp Ala Gln Phe Arg Thr Gln Ile Asn Glu Leu Lys

230 235 240 245

gaa aac att tac aac ggt gac atc tac caa gtt gtc ccg gcg cgc act 883

Glu Asn Ile Tyr Asn Gly Asp Ile Tyr Gln Val Val Pro Ala Arg Thr

250 255 260

ttc acc gca cca tgt cct gat gca ttc gct gct tat ctg cag ctg cgt 931

Phe Thr Ala Pro Cys Pro Asp Ala Phe Ala Ala Tyr Leu Gln Leu Arg

265 270 275

gcc acc aac ccg tcg ccg tac atg ttc tat atc cgt ggc ctc aac gaa 979

Ala Thr Asn Pro Ser Pro Tyr Met Phe Tyr Ile Arg Gly Leu Asn Glu

280 285 290

ggc cgc tcc tat gaa ctt ttt ggc gca tcc cct gag tcc aac ctc aag 1027

Gly Arg Ser Tyr Glu Leu Phe Gly Ala Ser Pro Glu Ser Asn Leu Lys

295 300 305

ttc acc gct gct aac cgt gag ctg cag ctg tac cca atc gca ggt acc 1075

Phe Thr Ala Ala Asn Arg Glu Leu Gln Leu Tyr Pro Ile Ala Gly Thr

310 315 320 325

cgc ccc cgt gga ctc aac cca gat ggc tcc atc aac gat gag cta gat 1123

Arg Pro Arg Gly Leu Asn Pro Asp Gly Ser Ile Asn Asp Glu Leu Asp

330 335 340

atc cgc aat gag ttg gat atg cgc act gat gcc aaa gag atc gcg gag 1171

Ile Arg Asn Glu Leu Asp Met Arg Thr Asp Ala Lys Glu Ile Ala Glu

345 350 355

cac acc atg ctt gtc gat ctc gcc cgc aac gac ctg gcc cgc gtc tcg 1219

His Thr Met Leu Val Asp Leu Ala Arg Asn Asp Leu Ala Arg Val Ser

360 365 370

gtc cca gcg tcg cgc cgg gtt gcg gat ctt ttg cag gtg gat cgc tat 1267

Val Pro Ala Ser Arg Arg Val Ala Asp Leu Leu Gln Val Asp Arg Tyr

375 380 385

tcc cgc gtg atg cac ttg gtg tcc cgt gtg acg gcg acg ttg gac cca 1315

Ser Arg Val Met His Leu Val Ser Arg Val Thr Ala Thr Leu Asp Pro

390 395 400 405

gag ctt gat gct ttg gac gcc tat cgg gcg tgc atg aat atg ggc acg 1363

Glu Leu Asp Ala Leu Asp Ala Tyr Arg Ala Cys Met Asn Met Gly Thr

410 415 420

ttg acc ggc gct ccg aag ttg cgc gct atg gag ctg ttg cgc ggc gtc 1411

Leu Thr Gly Ala Pro Lys Leu Arg Ala Met Glu Leu Leu Arg Gly Val

425 430 435

gaa aag cgc agg cgt ggt tct tat ggt ggg gca gtg ggg tac ctg cgc 1459

Glu Lys Arg Arg Arg Gly Ser Tyr Gly Gly Ala Val Gly Tyr Leu Arg

440 445 450

ggc aat ggc gat atg gat aat tgc att gtt att cgt tcg gcg ttt gtc 1507

Gly Asn Gly Asp Met Asp Asn Cys Ile Val Ile Arg Ser Ala Phe Val

455 460 465

cag gat ggt gtg gct gct gtg cag gct ggt gct ggt gtg gtc cgc gat 1555

Gln Asp Gly Val Ala Ala Val Gln Ala Gly Ala Gly Val Val Arg Asp

470 475 480 485

tct aat cct caa tct gaa gcc gat gag acg ttg cac aag gcg tat gcc 1603

Ser Asn Pro Gln Ser Glu Ala Asp Glu Thr Leu His Lys Ala Tyr Ala

490 495 500

gtg ttg aat gcc att gcg ctt gct gct ggt tcc act ttg gag gtc atc 1651

Val Leu Asn Ala Ile Ala Leu Ala Ala Gly Ser Thr Leu Glu Val Ile

505 510 515

cga tgacacacgt tgttctcatt gataatcacg 1684

Arg

<210>40

<211>518

<212>PRT

<213>谷氨酸棒状杆菌

<400>40

Met Ser Thr Asn Pro His Val Phe Ser Leu Asp Val Arg Tyr His Glu

1 5 10 15

Asp Ala Ser Ala Leu Phe Ala His Leu Gly Gly Thr Thr Ala Asp Asp

20 25 30

Ala Ala Leu Leu Glu Ser Ala Asp Ile Thr Thr Lys Asn Gly Ile Ser

35 40 45

Ser Leu Ala Val Leu Lys Ser Ser Val Arg Ile Thr Cys Thr Gly Asn

50 55 60

Thr Val Val Thr Gln Pro Leu Thr Asp Ser Gly Arg Ala Val Val Ala

65 70 75 80

Arg Leu Thr Gln Gln Leu Gly Gln Tyr Asn Thr Ala Glu Asn Thr Phe

85 90 95

Ser Phe Pro Ala Ser Asp Ala Val Asp Glu Arg Glu Arg Leu Thr Ala

100 105 110

Pro Ser Thr Ile Glu Val Leu Arg Lys Leu Gln Phe Glu Ser Gly Tyr

115 120 125

Ser Asp Ala Ser Leu Pro Leu Leu Met Gly Gly Phe Ala Phe Asp Phe

130 135 140

Leu Glu Thr Phe Glu Thr Leu Pro Ala Val Glu Glu Ser Val Asn Thr

145 150 155 160

Tyr Pro Asp Tyr Gln Phe Val Leu Ala Glu Ile Val Leu Asp Ile Asn

165 170 175

His Gln Asp Gln Thr Ala Lys Leu Ala Gly Val Ser Asn Ala Pro Gly

180 185 190

Glu Leu Glu Ala Glu Leu Asn Lys Leu Ser Leu Leu Ile Asp Ala Ala

195 200 205

Leu Pro Ala Thr Glu His Ala Tyr Gln Thr Thr Pro His Asp Gly Asp

210 215 220

Thr Leu Arg Val Val Ala Asp Ile Pro Asp Ala Gln Phe Arg Thr Gln

225 230 235 240

Ile Asn Glu Leu Lys Glu Asn Ile Tyr Asn Gly Asp Ile Tyr Gln Val

245 250 255

Val Pro Ala Arg Thr Phe Thr Ala Pro Cys Pro Asp Ala Phe Ala Ala

260 265 270

Tyr Leu Gln Leu Arg Ala Thr Asn Pro Ser Pro Tyr Met Phe Tyr Ile

275 280 285

Arg Gly Leu Asn Glu Gly Arg Ser Tyr Glu Leu Phe Gly Ala Ser Pro

290 295 300

Glu Ser Asn Leu Lys Phe Thr Ala Ala Asn Arg Glu Leu Gln Leu Tyr

305 310 315 320

Pro Ile Ala Gly Thr Arg Pro Arg Gly Leu Asn Pro Asp Gly Ser Ile

325 330 335

Asn Asp Glu Leu Asp Ile Arg Asn Glu Leu Asp Met Arg Thr Asp Ala

340 345 350

Lys Glu Ile Ala Glu His Thr Met Leu Val Asp Leu Ala Arg Asn Asp

355 360 365

Leu Ala Arg Val Ser Val Pro Ala Ser Arg Arg Val Ala Asp Leu Leu

370 375 380

Gln Val Asp Arg Tyr Ser Arg Val Met His Leu Val Ser Arg Val Thr

385 390 395 400

Ala Thr Leu Asp Pro Glu Leu Asp Ala Leu Asp Ala Tyr Arg Ala Cys

405 410 415

Met Asn Met Gly Thr Leu Thr Gly Ala Pro Lys Leu Arg Ala Met Glu

420 425 430

Leu Leu Arg Gly Val Glu Lys Arg Arg Arg Gly Ser Tyr Gly Gly Ala

435 440 445

Val Gly Tyr Leu Arg Gly Asn Gly Asp Met Asp Asn Cys Ile Val Ile

450 455 460

Arg Ser Ala Phe Val Gln Asp Gly Val Ala Ala Val Gln Ala Gly Ala

465 470 475 480

Gly Val Val Arg Asp Ser Asn Pro Gln Ser Glu Ala Asp Glu Thr Leu

485 490 495

His Lys Ala Tyr Ala Val Leu Asn Ala Ile Ala Leu Ala Ala Gly Ser

500 505 510

Thr Leu Glu Val Ile Arg

515

<210>41

<211>1057

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1027)

<223>RXA00970

<400>41

gaatctgatc tttcccgcac cgttgaactg ctgaaggcta agcctgttgt taaggcaatc 60

aacagtgtga tccgcctcga aagggactaa ttttactgac atg gca att gaa ctg 115

Met Ala Ile Glu Leu

1 5

aac gtc ggt cgt aag gtt acc gtc acg gta cct gga tct tct gca aac 163

Asn Val Gly Arg Lys Val Thr Val Thr Val Pro Gly Ser Ser Ala Asn

10 15 20

ctc gga cct ggc ttt gac act tta ggt ttg gca ctg tcg gta tac gac 211

Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala Leu Ser Val Tyr Asp

25 30 35

act gtc gaa gtg gaa att att cca tct ggc ttg gaa gtg gaa gtt ttt 259

Thr Val Glu Val Glu Ile Ile Pro Ser Gly Leu Glu Val Glu Val Phe

40 45 50

ggc gaa ggc caa ggc gaa gtc cct ctt gat ggc tcc cac ctg gtg gtt 307

Gly Glu Gly Gln Gly Glu Val Pro Leu Asp Gly Ser His Leu Val Val

55 60 65

aaa gct att cgt gct ggc ctg aag gca gct gac gct gaa gtt cct gga 355

Lys Ala Ile Arg Ala Gly Leu Lys Ala Ala Asp Ala Glu Val Pro Gly

70 75 80 85

ttg cga gtg gtg tgc cac aac aac att ccg cag tct cgt ggt ctt ggc 403

Leu Arg Val Val Cys His Asn Asn Ile Pro Gln Ser Arg Gly Leu Gly

90 95 100

tcc tct gct gca gcg gcg gtt gct ggt gtt gct gca gct aat ggt ttg 451

Ser Ser Ala Ala Ala Ala Val Ala Gly Val Ala Ala Ala Asn Gly Leu

105 110 115

gcg gat ttc ccg ctg act caa gag cag att gtt cag ttg tcc tct gcc 499

Ala Asp Phe Pro Leu Thr Gln Glu Gln Ile Val Gln Leu Ser Ser Ala

120 125 130

ttt gaa ggc cac cca gat aat gct gcg gct tct gtg ctg ggt gga gca 547

Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser Val Leu Gly Gly Ala

135 140 145

gtg gtg tcg tgg aca aat ctg tct atc gac ggc aag agc cag cca cag 595

Val Val Ser Trp Thr Asn Leu Ser Ile Asp Gly Lys Ser Gln Pro Gln

150 155 160 165

tat gct gct gta cca ctt gag gtg cag gac aat att cgt gcg act gcg 643

Tyr Ala Ala Val Pro Leu Glu Val Gln Asp Asn Ile Arg Ala Thr Ala

170 175 180

ctg gtt cct aat ttc cac gca tcc acc gaa gct gtg cgc cga gtc ctt 691

Leu Val Pro Asn Phe His Ala Ser Thr Glu Ala Val Arg Arg Val Leu

185 190 195

ccc act gaa gtc act cac atc gat gcg cga ttt aac gtg tcc cgc gtt 739

Pro Thr Glu Val Thr His Ile Asp Ala Arg Phe Asn Val Ser Arg Val

200 205 210

gca gtg atg atc gtt gcg ttg cag cag cgt cct gat ttg ctg tgg gag 787

Ala Val Met Ile Val Ala Leu Gln Gln Arg Pro Asp Leu Leu Trp Glu

215 220 225

ggt act cgt gac cgt ctg cac cag cct tat cgt gca gaa gtg ttg cct 835

Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg Ala Glu Val Leu Pro

230 235 240 245

att acc tct gag tgg gta aac cgc ctg cgc aac cgt ggc tac gcg gca 883

Ile Thr Ser Glu Trp Val Asn Arg Leu Arg Asn Arg Gly Tyr Ala Ala

250 255 260

tac ctt tcc ggt gcc ggc cca acc gcc atg gtg ctg tcc act gag cca 931

Tyr Leu Ser Gly Ala Gly Pro Thr Ala Met Val Leu Ser Thr Glu Pro

265 270 275

att cca gac aag gtt ttg gaa gat gct cgt gag tct ggc att aag gtg 979

Ile Pro Asp Lys Val Leu Glu Asp Ala Arg Glu Ser Gly Ile Lys Val

280 285 290

ctt gag ctt gag gtt gcg gga cca gtc aag gtt gaa gtt aac caa cct 1027

Leu Glu Leu Glu Val Ala Gly Pro Val Lys Val Glu Val Asn Gln Pro

295 300 305

taggcccaac aaggaaggcc cccttcgaat 1057

<210>42

<211>309

<212>PRT

<213>谷氨酸棒状杆菌

<400>42

Met Ala Ile Glu Leu Asn Val Gly Arg Lys Val Thr Val Thr Val Pro

1 5 10 15

Gly Ser Ser Ala Asn Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala

20 25 30

Leu Ser Val Tyr Asp Thr Val Glu Val Glu Ile Ile Pro Ser Gly Leu

35 40 45

Glu Val Glu Val Phe Gly Glu Gly Gln Gly Glu Val Pro Leu Asp Gly

50 55 60

Ser His Leu Val Val Lys Ala Ile Arg Ala Gly Leu Lys Ala Ala Asp

65 70 75 80

Ala Glu Val Pro Gly Leu Arg Val Val Cys His Asn Asn Ile Pro Gln

85 90 95

Ser Arg Gly Leu Gly Ser Ser Ala Ala Ala Ala Val Ala Gly Val Ala

100 105 110

Ala Ala Asn Gly Leu Ala Asp Phe Pro Leu Thr Gln Glu Gln Ile Val

115 120 125

Gln Leu Ser Ser Ala Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser

130 135 140

Val Leu Gly Gly Ala Val Val Ser Trp Thr Asn Leu Ser Ile Asp Gly

145 150 155 160

Lys Ser Gln Pro Gln Tyr Ala Ala Val Pro Leu Glu Val Gln Asp Asn

165 170 175

Ile Arg Ala Thr Ala Leu Val Pro Asn Phe His Ala Ser Thr Glu Ala

180 185 190

Val Arg Arg Val Leu Pro Thr Glu Val Thr His Ile Asp Ala Arg Phe

195 200 205

Asn Val Ser Arg Val Ala Val Met Ile Val Ala Leu Gln Gln Arg Pro

210 215 220

Asp Leu Leu Trp Glu Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg

225 230 235 240

Ala Glu Val Leu Pro Ile Thr Ser Glu Trp Val Asn Arg Leu Arg Asn

245 250 255

Arg Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Ala Met Val

260 265 270

Leu Ser Thr Glu Pro Ile Pro Asp Lys Val Leu Glu Asp Ala Arg Glu

275 280 285

Ser Gly Ile Lys Val Leu Glu Leu Glu Val Ala Gly Pro Val Lys Val

290 295 300

Glu Val Asn Gln Pro

305

<210>43

<211>793

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(763)

<223>RXA00997

<400>43

acaacaacac cgacttcatt tctgctgtga gctccggtgt catcgtggcc gaagcaacgc 60

cgattcagct aggtggccgc acccatctgt ggcagatcga atg cac cca ccg cgg 115

Met His Pro Pro Arg

1 5

aga agt cgt cgc acg aac cac act gcg cac cat ggt gct gaa caa gta 163

Arg Ser Arg Arg Thr Asn His Thr Ala His His Gly Ala Glu Gln Val

10 15 20

gcc cta tac tcg ggc acc atg act aca tgg aaa gag ctc aca gat aac 211

Ala Leu Tyr Ser Gly Thr Met Thr Thr Trp Lys Glu Leu Thr Asp Asn

25 30 35

aac cca gcg cac tca gaa aac tac gcg cag cgc tgg cga aac ctc gcc 259

Asn Pro Ala His Ser Glu Asn Tyr Ala Gln Arg Trp Arg Asn Leu Ala

40 45 50

gca gca ggc aat gat att tac ggc gaa gcc cgc ctc att gat gcc atg 307

Ala Ala Gly Asn Asp Ile Tyr Gly Glu Ala Arg Leu Ile Asp Ala Met

55 60 65

gca ccc agg gga gcg aaa atc ttg gat gct ggc tgc ggc cag gga cgc 355

Ala Pro Arg Gly Ala Lys Ile Leu Asp Ala Gly Cys Gly Gln Gly Arg

70 75 80 85

atc ggt ggc tac ctg tcc aag caa ggc cac gat gtt cta ggc aca gac 403

Ile Gly Gly Tyr Leu Ser Lys Gln Gly His Asp Val Leu Gly Thr Asp

90 95 100

ctt gat ccc atc ctg att gat tac gcc aag cag gac ttt cca gaa gct 451

Leu Asp Pro Ile Leu Ile Asp Tyr Ala Lys Gln Asp Phe Pro Glu Ala

105 110 115

cgc tgg gtg gtg gga gat ctc tct gtt gat cag atc tca gag act gat 499

Arg Trp Val Val Gly Asp Leu Ser Val Asp Gln Ile Ser Glu Thr Asp

120 125 130

ttt gat ctc att gtc tcc gcc ggc aac gtc atg ggc ttt ctc gct gag 547

Phe Asp Leu Ile Val Ser Ala Gly Asn Val Met Gly Phe Leu Ala Glu

135 140 145

gat ggt cgc gaa cct gca cta gcc aac att cac cgc gca ctg ggc gcc 595

Asp Gly Arg Glu Pro Ala Leu Ala Asn Ile His Arg Ala Leu Gly Ala

150 155 160 165

gat ggc cgc gct gtc atc ggt ttc ggc gca gga cgt gga tgg gtc ttt 643

Asp Gly Arg Ala Val Ile Gly Phe Gly Ala Gly Arg Gly Trp Val Phe

170 175 180

gga gac ttc ctc gaa gtc gca gaa cgc gtg ggc ctc gag ttg gaa aat 691

Gly Asp Phe Leu Glu Val Ala Glu Arg Val Gly Leu Glu Leu Glu Asn

185 190 195

gct ttt gag tct tgg gat ctt aag cct ttt gtc caa ggc tct gag ttc 739

Ala Phe Glu Ser Trp Asp Leu Lys Pro Phe Val Gln Gly Ser Glu Phe

200 205 210

tta gtg gcg gta ttt acc aag aag taacacctct atcttgcacc tgatctggcg 793

Leu Val Ala Val Phe Thr Lys Lys

215 220

<210>44

<211>221

<212>PRT

<213>谷氨酸棒状杆菌

<400>44

Met His Pro Pro Arg Arg Ser Arg Arg Thr Asn His Thr Ala His His

1 5 10 15

Gly Ala Glu Gln Val Ala Leu Tyr Ser Gly Thr Met Thr Thr Trp Lys

20 25 30

Glu Leu Thr Asp Asn Asn Pro Ala His Ser Glu Asn Tyr Ala Gln Arg

35 40 45

Trp Arg Asn Leu Ala Ala Ala Gly Asn Asp Ile Tyr Gly Glu Ala Arg

50 55 60

Leu Ile Asp Ala Met Ala Pro Arg Gly Ala Lys Ile Leu Asp Ala Gly

65 70 75 80

Cys Gly Gln Gly Arg Ile Gly Gly Tyr Leu Ser Lys Gln Gly His Asp

85 90 95

Val Leu Gly Thr Asp Leu Asp Pro Ile Leu Ile Asp Tyr Ala Lys Gln

100 105 110

Asp Phe Pro Glu Ala Arg Trp Val Val Gly Asp Leu Ser Val Asp Gln

115 120 125

Ile Ser Glu Thr Asp Phe Asp Leu Ile Val Ser Ala Gly Asn Val Met

130 135 140

Gly Phe Leu Ala Glu Asp Gly Arg Glu Pro Ala Leu Ala Asn Ile His

145 150 155 160

Arg Ala Leu Gly Ala Asp Gly Arg Ala Val Ile Gly Phe Gly Ala Gly

165 170 175

Arg Gly Trp Val Phe Gly Asp Phe Leu Glu Val Ala Glu Arg Val Gly

180 185 190

Leu Glu Leu Glu Asn Ala Phe Glu Ser Trp Asp Leu Lys Pro Phe Val

195 200 205

Gln Gly Ser Glu Phe Leu Val Ala Val Phe Thr Lys Lys

210 215 220

<210>45

<211>931

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(901)

<223>RXA01095

<400>45

aagactcaga aatccaaaga aaccccaggt caaagctagg gtgtggcacc ctgatttctt 60

tcgccatgtg tgttcgggat aaccttaaac acagcattgg ttg gaa gga ggt tgg 115

Leu Glu Gly Gly Trp

1 5

ggc atg gtt gca aca gag aac cgc atg ttg atg gaa atc gct gcg gaa 163

Gly Met Val Ala Thr Glu Asn Arg Met Leu Met Glu Ile Ala Ala Glu

10 15 20

ata tcg gct cgg gaa gca acg ctt ggt ttt caa gaa gtc aaa act aaa 211

Ile Ser Ala Arg Glu Ala Thr Leu Gly Phe Gln Glu Val Lys Thr Lys

25 30 35

tct cga tca gca ggt ctc acg gcg gct ttc gat att gct tca gtc ttt 259

Ser Arg Ser Ala Gly Leu Thr Ala Ala Phe Asp Ile Ala Ser Val Phe

40 45 50

ttt tcg tct gga tgt aat gtc gta gtc gcc ttt gat cgt ttt gca tcc 307

Phe Ser Ser Gly Cys Asn Val Val Val Ala Phe Asp Arg Phe Ala Ser

55 60 65

aat tgg tct gat cat tcg gat cat gtg gac tac gct gca cag gtt gcg 355

Asn Trp Ser Asp His Ser Asp His Val Asp Tyr Ala Ala Gln Val Ala

70 75 80 85

ggt ttt ggc gca tca atg ctt gca tat acg gtg cgc agg gga cag ttt 403

Gly Phe Gly Ala Ser Met Leu Ala Tyr Thr Val Arg Arg Gly Gln Phe

90 95 100

gat acc gca gta cgc gat atc agg gac atc aaa tct gaa gta gac att 451

Asp Thr Ala Val Arg Asp Ile Arg Asp Ile Lys Ser Glu Val Asp Ile

105 110 115

ccc att ctg ctt cat gat ccc atc atc gat ccg tat caa atc cac gaa 499

Pro Ile Leu Leu His Asp Pro Ile Ile Asp Pro Tyr Gln Ile His Glu

120 125 130

gcc cgc gtc atg ggc atc gac gct ctt caa ttc ccc gta tgg gcg atg 547

Ala Arg Val Met Gly Ile Asp Ala Leu Gln Phe Pro Val Trp Ala Met

135 140 145

gaa caa gct cga ctg gaa tct ttg gtg gac cgc acc gaa tca ttg ggc 595

Glu Gln Ala Arg Leu Glu Ser Leu Val Asp Arg Thr Glu Ser Leu Gly

150 155 160 165

atg aca gcc atc gtg tct gtg cga aac cac gaa gaa gcg cat cgt gca 643

Met Thr Ala Ile Val Ser Val Arg Asn His Glu Glu Ala His Arg Ala

170 175 180

gtg gac gca gga gcg aca gtg gta gca att gat att act ggt tat acc 691

Val Asp Ala Gly Ala Thr Val Val Ala Ile Asp Ile Thr Gly Tyr Thr

185 190 195

ggc tca ctc act ttg cct gaa gcg ttt tcg ggt atc acc caa ttc atg 739

Gly Ser Leu Thr Leu Pro Glu Ala Phe Ser Gly Ile Thr Gln Phe Met

200 205 210

ccc aaa gag gta gcc cgc att gtg ctc gga ggt tgc agc agc cct aaa 787

Pro Lys Glu Val Ala Arg Ile Val Leu Gly Gly Cys Ser Ser Pro Lys

215 220 225

gaa ctc atg cgg ttt gca cga cat tct gca gac gcc atc ttt gtt cca 835

Glu Leu Met Arg Phe Ala Arg His Ser Ala Asp Ala Ile Phe Val Pro

230 235 240 245

cat gca gac ctc gcc acc aca aaa tct ctt gtg aca gca ggt atg cat 883

His Ala Asp Leu Ala Thr Thr Lys Ser Leu Val Thr Ala Gly Met His

250 255 260

cca gcg tgc cca tcg cgt tgaagaggtg ctctgtggtc agctgaccgg 931

Pro Ala Cys Pro Ser Arg

265

<210>46

<211>267

<212>PRT

<213>谷氨酸棒状杆菌

<400>46

Leu Glu Gly Gly Trp Gly Met Val Ala Thr Glu Asn Arg Met Leu Met

1 5 10 15

Glu Ile Ala Ala Glu Ile Ser Ala Arg Glu Ala Thr Leu Gly Phe Gln

20 25 30

Glu Val Lys Thr Lys Ser Arg Ser Ala Gly Leu Thr Ala Ala Phe Asp

35 40 45

Ile Ala Ser Val Phe Phe Ser Ser Gly Cys Asn Val Val Val Ala Phe

50 55 60

Asp Arg Phe Ala Ser Asn Trp Ser Asp His Ser Asp His Val Asp Tyr

65 70 75 80

Ala Ala Gln Val Ala Gly Phe Gly Ala Ser Met Leu Ala Tyr Thr Val

85 90 95

Arg Arg Gly Gln Phe Asp Thr Ala Val Arg Asp Ile Arg Asp Ile Lys

100 105 110

Ser Glu Val Asp Ile Pro Ile Leu Leu His Asp Pro Ile Ile Asp Pro

115 120 125

Tyr Gln Ile His Glu Ala Arg Val Met Gly Ile Asp Ala Leu Gln Phe

130 135 140

Pro Val Trp Ala Met Glu Gln Ala Arg Leu Glu Ser Leu Val Asp Arg

145 150 155 160

Thr Glu Ser Leu Gly Met Thr Ala Ile Val Ser Val Arg Asn His Glu

165 170 175

Glu Ala His Arg Ala Val Asp Ala Gly Ala Thr Val Val Ala Ile Asp

180 185 190

Ile Thr Gly Tyr Thr Gly Ser Leu Thr Leu Pro Glu Ala Phe Ser Gly

195 200 205

Ile Thr Gln Phe Met Pro Lys Glu Val Ala Arg Ile Val Leu Gly Gly

210 215 220

Cys Ser Ser Pro Lys Glu Leu Met Arg Phe Ala Arg His Ser Ala Asp

225 230 235 240

Ala Ile Phe Val Pro His Ala Asp Leu Ala Thr Thr Lys Ser Leu Val

245 250 255

Thr Ala Gly Met His Pro Ala Cys Pro Ser Arg

260 265

<210>47

<211>1228

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1198)

<223>RXA01105

<400>47

ttgtcatcaa ctttgccaac gccgaagatc ttccagcgca cggcgaagca atccgtgcac 60

gctttgaaaa cctccccacc accgacgagg cctaagaaaa atg acc aaa att act 115

Met Thr Lys Ile Thr

1 5

ttg agc gat ttg cca ttg cgt gaa gaa ctg cgc ggt gag cac gct tac 163

Leu Ser Asp Leu Pro Leu Arg Glu Glu Leu Arg Gly Glu His Ala Tyr

10 15 20

ggc gca ccc cag ctc aac gtt gat att cgc ctc aac acc aac gaa aac 211

Gly Ala Pro Gln Leu Asn Val Asp Ile Arg Leu Asn Thr Asn Glu Asn

25 30 35

cct tac cca ccg tca gag gca ttg gtc gct gac ttg gtt gcc acc gtg 259

Pro Tyr Pro Pro Ser Glu Ala Leu Val Ala Asp Leu Val Ala Thr Val

40 45 50

gat aag atc gcc acc gag ctg aac cgc tac cca gag cgc gat gct gtg 307

Asp Lys Ile Ala Thr Glu Leu Asn Arg Tyr Pro Glu Arg Asp Ala Val

55 60 65

gaa ctg cgt gat gag ttg gct gcg tac atc acc aag caa acc ggc gtg 355

Glu Leu Arg Asp Glu Leu Ala Ala Tyr Ile Thr Lys Gln Thr Gly Val

70 75 80 85

gct gtc acc agg gat aac ctg tgg gct gcc aat ggt tcc aat gaa att 403

Ala Val Thr Arg Asp Asn Leu Trp Ala Ala Asn Gly Ser Asn Glu Ile

90 95 100

ctg cag cag ctg ctg cag gct ttt ggt gga cct gga cgc acc gcg ttg 451

Leu Gln Gln Leu Leu Gln Ala Phe Gly Gly Pro Gly Arg Thr Ala Leu

105 110 115

gga ttc caa ccc agc tat tcc atg cac cca att ttg gct aaa ggc acc 499

Gly Phe Gln Pro Ser Tyr Ser Met His Pro Ile Leu Ala Lys Gly Thr

120 125 130

cac act gaa ttc att gcg gtg tcc cga ggt gct gat ttc cgc atc gat 547

His Thr Glu Phe Ile Ala Val Ser Arg Gly Ala Asp Phe Arg Ile Asp

135 140 145

atg gat gtg gcg ctg gaa gaa att cgt gca aag cag cct gac att gtt 595

Met Asp Val Ala Leu Glu Glu Ile Arg Ala Lys Gln Pro Asp Ile Val

150 155 160 165

ttt gtc acc acc ccg aac aac ccg acc ggt gat gtg acc tcg ctg gac 643

Phe Val Thr Thr Pro Asn Asn Pro Thr Gly Asp Val Thr Ser Leu Asp

170 175 180

gat gtt gag cgc atc atc aac gtt gcc cca ggc atc gtg atc gtg gat 691

Asp Val Glu Arg Ile Ile Asn Val Ala Pro Gly Ile Val Ile Val Asp

185 190 195

gaa gct tat gcg gaa ttc tcc cca tca cct tca gca acc act ctt ctg 739

Glu Ala Tyr Ala Glu Phe Ser Pro Ser Pro Ser Ala Thr Thr Leu Leu

200 205 210

gag aag tac cca acc aag ctg gtg gtg tcc cgc acc atg agt aag gct 787

Glu Lys Tyr Pro Thr Lys Leu Val Val Ser Arg Thr Met Ser Lys Ala

215 220 225

ttt gat ttc gca ggt gga cgc ctc ggc tac ttc gtg gcc aac cca gcg 835

Phe Asp Phe Ala Gly Gly Arg Leu Gly Tyr Phe Val Ala Asn Pro Ala

230 235 240 245

ttt atc gac gcc gtg atg cta gtc cgc ctt ccg tat cat ctt tca gcg 883

Phe Ile Asp Ala Val Met Leu Val Arg Leu Pro Tyr His Leu Ser Ala

250 255 260

ctg agc caa gca gcc gca atc gta gcg ctg cgt cac tcc gct gac acg 931

Leu Ser Gln Ala Ala Ala Ile Val Ala Leu Arg His Ser Ala Asp Thr

265 270 275

ctg gga acc gtc gaa aag ctc tct gta gag cgt gtt cgc gtg gca gca 979

Leu Gly Thr Val Glu Lys Leu Ser Val Glu Arg Val Arg Val Ala Ala

280 285 290

cgc ttg gag gaa ctg ggc tac gct gtg gtt cca agt gag tcc aac ttt 1027

Arg Leu Glu Glu Leu Gly Tyr Ala Val Val Pro Ser Glu Ser Asn Phe

295 300 305

gtg ttc ttt gga gat ttc tcc gat cag cac gcg gca tgg cag gca ttt 1075

Val Phe Phe Gly Asp Phe Ser Asp Gln His Ala Ala Trp Gln Ala Phe

310 315 320 325

ttg gat agg gga gtg ctc atc cgc gat gtg gga atc gct ggg cac ttg 1123

Leu Asp Arg Gly Val Leu Ile Arg Asp Val Gly Ile Ala Gly His Leu

330 335 340

cgc act acc att ggt gtg cct gag gaa aat gat gcg ttt ttg gac gca 1171

Arg Thr Thr Ile Gly Val Pro Glu Glu Asn Asp Ala Phe Leu Asp Ala

345 350 355

gct gca gag atc atc aag ctg aac ctg taagagagaa gaatttttca 1218

Ala Ala Glu Ile Ile Lys Leu Asn Leu

360 365

tgactgtcgc 1228

<210>48

<211>366

<212>PRT

<213>谷氨酸棒状杆菌

<400>48

Met Thr Lys Ile Thr Leu Ser Asp Leu Pro Leu Arg Glu Glu Leu Arg

1 5 10 15

Gly Glu His Ala Tyr Gly Ala Pro Gln Leu Asn Val Asp Ile Arg Leu

20 25 30

Asn Thr Asn Glu Asn Pro Tyr Pro Pro Ser Glu Ala Leu Val Ala Asp

35 40 45

Leu Val Ala Thr Val Asp Lys Ile Ala Thr Glu Leu Asn Arg Tyr Pro

50 55 60

Glu Arg Asp Ala Val Glu Leu Arg Asp Glu Leu Ala Ala Tyr Ile Thr

65 70 75 80

Lys Gln Thr Gly Val Ala Val Thr Arg Asp Asn Leu Trp Ala Ala Asn

85 90 95

Gly Ser Asn Glu Ile Leu Gln Gln Leu Leu Gln Ala Phe Gly Gly Pro

100 105 110

Gly Arg Thr Ala Leu Gly Phe Gln Pro Ser Tyr Ser Met His Pro Ile

115 120 125

Leu Ala Lys Gly Thr His Thr Glu Phe Ile Ala Val Ser Arg Gly Ala

130 135 140

Asp Phe Arg Ile Asp Met Asp Val Ala Leu Glu Glu Ile Arg Ala Lys

145 150 155 160

Gln Pro Asp Ile Val Phe Val Thr Thr Pro Asn Asn Pro Thr Gly Asp

165 170 175

Val Thr Ser Leu Asp Asp Val Glu Arg Ile Ile Asn Val Ala Pro Gly

180 185 190

Ile Val Ile Val Asp Glu Ala Tyr Ala Glu Phe Ser Pro Ser Pro Ser

195 200 205

Ala Thr Thr Leu Leu Glu Lys Tyr Pro Thr Lys Leu Val Val Ser Arg

210 215 220

Thr Met Ser Lys Ala Phe Asp Phe Ala Gly Gly Arg Leu Gly Tyr Phe

225 230 235 240

Val Ala Asn Pro Ala Phe Ile Asp Ala Val Met Leu Val Arg Leu Pro

245 250 255

Tyr His Leu Ser Ala Leu Ser Gln Ala Ala Ala Ile Val Ala Leu Arg

260 265 270

His Ser Ala Asp Thr Leu Gly Thr Val Glu Lys Leu Ser Val Glu Arg

275 280 285

Val Arg Val Ala Ala Arg Leu Glu Glu Leu Gly Tyr Ala Val Val Pro

290 295 300

Ser Glu Ser Asn Phe Val Phe Phe Gly Asp Phe Ser Asp Gln His Ala

305 310 315 320

Ala Trp Gln Ala Phe Leu Asp Arg Gly Val Leu Ile Arg Asp Val Gly

325 330 335

Ile Ala Gly His Leu Arg Thr Thr Ile Gly Val Pro Glu Glu Asn Asp

340 345 350

Ala Phe Leu Asp Ala Ala Ala Glu Ile Ile Lys Leu Asn Leu

355 360 365

<210>49

<211>883

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(853)

<223>RXA01115

<400>49

ctcaccgcat gcagcgtgaa aacactcagt acggactggc caccatgtgc atcggtggcg 60

gccagggtct tgcagctgtc tttgaaaagg agaactaaaa atg gct att ttg cac 115

Met Ala Ile Leu His

1 5

agc gtt tcc tac gga act tcc gac aac acc ttg gtg ttc att ggc tcg 163

Ser Val Ser Tyr Gly Thr Ser Asp Asn Thr Leu Val Phe Ile Gly Ser

10 15 20

ttg ggt tcc acc acc gac atg tgg ctg cca cag ctg gat gcc ttg cat 211

Leu Gly Ser Thr Thr Asp Met Trp Leu Pro Gln Leu Asp Ala Leu His

25 30 35

aag gat ttc cgc gtc atc gct gtt gat cac cgc gga cat ggt ctg tct 259

Lys Asp Phe Arg Val Ile Ala Val Asp His Arg Gly His Gly Leu Ser

40 45 50

gaa ctc atc gaa ggc acc ccc act gtg gcg gat ctg gcg cag gat gtg 307

Glu Leu Ile Glu Gly Thr Pro Thr Val Ala Asp Leu Ala Gln Asp Val

55 60 65

ctg gat acc ctc gat gac ctg ggt gtc gga aac ttc ggc gtc atc gga 355

Leu Asp Thr Leu Asp Asp Leu Gly Val Gly Asn Phe Gly Val Ile Gly

70 75 80 85

cta tct ctc ggc gga gcg gtt gca caa tac ttg gcg gcc acc tct gat 403

Leu Ser Leu Gly Gly Ala Val Ala Gln Tyr Leu Ala Ala Thr Ser Asp

90 95 100

cgt gtc acc aag gca gca ttc atg tgt acc gct gca aaa ttc ggc gag 451

Arg Val Thr Lys Ala Ala Phe Met Cys Thr Ala Ala Lys Phe Gly Glu

105 110 115

ccc cag ggc tgg cta gat cgc gcc gca gcg tgc cgc gaa aac ggc act 499

Pro Gln Gly Trp Leu Asp Arg Ala Ala Ala Cys Arg Glu Asn Gly Thr

120 125 130

ggt tct ctg tcc gaa gct gtg atc cag cgc tgg ttc tcc ccc act tgg 547

Gly Ser Leu Ser Glu Ala Val Ile Gln Arg Trp Phe Ser Pro Thr Trp

135 140 145

ttg gag aac aac cca gcg tcc cgc gag cac ttc gaa gcc atg gtt gcc 595

Leu Glu Asn Asn Pro Ala Ser Arg Glu His Phe Glu Ala Met Val Ala

150 155 160 165

ggc acc cca tct gag ggt tac gcg ctg tgc tgc gag gcg ttg gca acc 643

Gly Thr Pro Ser Glu Gly Tyr Ala Leu Cys Cys Glu Ala Leu Ala Thr

170 175 180

tgg gat ttc acc gat cgc ctg gga gaa atc acc gtg cca gtg ctc acc 691

Trp Asp Phe Thr Asp Arg Leu Gly Glu Ile Thr Val Pro Val Leu Thr

185 190 195

atc gca ggt gcc gat gac ccc tcc act cct cca gca acc gtg cag atc 739

Ile Ala Gly Ala Asp Asp Pro Ser Thr Pro Pro Ala Thr Val Gln Ile

200 205 210

att gcc gat ggc gtt ggc ggc gag tcc cgc gca gag gtc cta agc cca 787

Ile Ala Asp Gly Val Gly Gly Glu Ser Arg Ala Glu Val Leu Ser Pro

215 220 225

gcc gcg cac gta cca acc gtg gaa cgt cca aac gag gta aat gaa ctg 835

Ala Ala His Val Pro Thr Val Glu Arg Pro Asn Glu Val Asn Glu Leu

230 235 240 245

cta gca cag cat ttc gct taatgttgta ggcatgttca caaccagtct 883

Leu Ala Gln His Phe Ala

250

<210>50

<211>251

<212>PRT

<213>谷氨酸棒状杆菌

<400>50

Met Ala Ile Leu His Ser Val Ser Tyr Gly Thr Ser Asp Asn Thr Leu

1 5 10 15

Val Phe Ile Gly Ser Leu Gly Ser Thr Thr Asp Met Trp Leu Pro Gln

20 25 30

Leu Asp Ala Leu His Lys Asp Phe Arg Val Ile Ala Val Asp His Arg

35 40 45

Gly His Gly Leu Ser Glu Leu Ile Glu Gly Thr Pro Thr Val Ala Asp

50 55 60

Leu Ala Gln Asp Val Leu Asp Thr Leu Asp Asp Leu Gly Val Gly Asn

65 70 75 80

Phe Gly Val Ile Gly Leu Ser Leu Gly Gly Ala Val Ala Gln Tyr Leu

85 90 95

Ala Ala Thr Ser Asp Arg Val Thr Lys Ala Ala Phe Met Cys Thr Ala

100 105 110

Ala Lys Phe Gly Glu Pro Gln Gly Trp Leu Asp Arg Ala Ala Ala Cys

115 120 125

Arg Glu Asn Gly Thr Gly Ser Leu Ser Glu Ala Val Ile Gln Arg Trp

130 135 140

Phe Ser Pro Thr Trp Leu Glu Asn Asn Pro Ala Ser Arg Glu His Phe

145 150 155 160

Glu Ala Met Val Ala Gly Thr Pro Ser Glu Gly Tyr Ala Leu Cys Cys

165 170 175

Glu Ala Leu Ala Thr Trp Asp Phe Thr Asp Arg Leu Gly Glu Ile Thr

180 185 190

Val Pro Val Leu Thr Ile Ala Gly Ala Asp Asp Pro Ser Thr Pro Pro

195 200 205

Ala Thr Val Gln Ile Ile Ala Asp Gly Val Gly Gly Glu Ser Arg Ala

210 215 220

Glu Val Leu Ser Pro Ala Ala His Val Pro Thr Val Glu Arg Pro Asn

225 230 235 240

Glu Val Asn Glu Leu Leu Ala Gln His Phe Ala

245 250

<210>51

<211>1171

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1141)

<223>RXA01127

<400>51

aacgatttcc agttgagtca agccctgcat gatggggtag tgggggttgt tgggcaggta 60

cgagctgtga tcaatcagct acactagtga agtccatata gtg aga agg gaa tcc 115

Val Arg Arg Glu Ser

1 5

cac aac atg aaa ctt gct gtt att ggt gga gat ggt atc ggc cca gag 163

His Asn Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu

10 15 20

gtt act gca gaa gcc ctc aag gtt cta aac gct gtc cgc gac gac atc 211

Val Thr Ala Glu Ala Leu Lys Val Leu Asn Ala Val Arg Asp Asp Ile

25 30 35

gag acc acc gat tat gac ctt ggc gca cgc cgt tac ctc aaa aat ggc 259

Glu Thr Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Lys Asn Gly

40 45 50

gag ctg ctc acc gac gag gat ctg gca tcc ctg cgc gag cat gac gcg 307

Glu Leu Leu Thr Asp Glu Asp Leu Ala Ser Leu Arg Glu His Asp Ala

55 60 65

atc ctt ctt ggc gct atc ggt gca cca ggt tcc gtc cct cca gga att 355

Ile Leu Leu Gly Ala Ile Gly Ala Pro Gly Ser Val Pro Pro Gly Ile

70 75 80 85

ctc gag cgt ggt ttg ctg ctg aag atg cga ttc gca ctg gat cac cac 403

Leu Glu Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His

90 95 100

gtg aac ctg cgc cca tcc aag ctg tac gac ggc gtg gag tcc cca ctg 451

Val Asn Leu Arg Pro Ser Lys Leu Tyr Asp Gly Val Glu Ser Pro Leu

105 110 115

cgt aac cca ggc aag att gat ttc gtt gtg gtc cgc gaa ggt acc gaa 499

Arg Asn Pro Gly Lys Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu

120 125 130

ggc gcc tac act ggc aac ggt gga gca atc cgc gtg gga acc cct cac 547

Gly Ala Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His

135 140 145

gag att gcc aat gaa acc tcc gtg aac act cgc tac ggc gct gag cgc 595

Glu Ile Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg

150 155 160 165

gtt att cgc tac gca ttc gag ctg gca cag agc cgc cgc aag aag ctc 643

Val Ile Arg Tyr Ala Phe Glu Leu Ala Gln Ser Arg Arg Lys Lys Leu

170 175 180

acc ctc gtg cac aag acc aac gtc ctg gtt cac ggt ggt ggc ctg tgg 691

Thr Leu Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Leu Trp

185 190 195

cag cgc acc gta gat gag gtt gca aag gaa tac cca gag gta gcc gtc 739

Gln Arg Thr Val Asp Glu Val Ala Lys Glu Tyr Pro Glu Val Ala Val

200 205 210

gat tac aac cac atc gat gca gca acc atc tat ctg gtc act gat cct 787

Asp Tyr Asn His Ile Asp Ala Ala Thr Ile Tyr Leu Val Thr Asp Pro

215 220 225

tcc cgc ttc gat gtg att gtt acc gat aac ctc ttc ggc gac atc ctc 835

Ser Arg Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Leu

230 235 240 245

acc gat gag gca ggc gca gtc tct ggc gga att ggc ctc gca gca tcc 883

Thr Asp Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser

250 255 260

ggc aac atc gat gcc acg ggc acc aac cct tcc atg ttc gag cca gtc 931

Gly Asn Ile Asp Ala Thr Gly Thr Asn Pro Ser Met Phe Glu Pro Val

265 270 275

cac ggc tct gca cca gat atc gca ggc cag gga atc gca gac cca acg 979

His Gly Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr

280 285 290

gca gca atc cta tcc gct gcg atg ctg ctg cgt cac tta ggt gat gaa 1027

Ala Ala Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu

295 300 305

gac aac gca gta cgt att gaa aca gcc atc gca gct gat gtg gct ggc 1075

Asp Asn Ala Val Arg Ile Glu Thr Ala Ile Ala Ala Asp Val Ala Gly

310 315 320 325

cga gat aac tct cag ccg att tct acc act gag gtg gga gac cgc atc 1123

Arg Asp Asn Ser Gln Pro Ile Ser Thr Thr Glu Val Gly Asp Arg Ile

330 335 340

gtc aag gcg ctg caa agc taaatttcaa cgccgacccc cttgcctttt 1171

Val Lys Ala Leu Gln Ser

345

<210>52

<211>347

<212>PRT

<213>谷氨酸棒状杆菌

<400>52

Val Arg Arg Glu Ser His Asn Met Lys Leu Ala Val Ile Gly Gly Asp

1 5 10 15

Gly Ile Gly Pro Glu Val Thr Ala Glu Ala Leu Lys Val Leu Asn Ala

20 25 30

Val Arg Asp Asp Ile Glu Thr Thr Asp Tyr Asp Leu Gly Ala Arg Arg

35 40 45

Tyr Leu Lys Asn Gly Glu Leu Leu Thr Asp Glu Asp Leu Ala Ser Leu

50 55 60

Arg Glu His Asp Ala Ile Leu Leu Gly Ala Ile Gly Ala Pro Gly Ser

65 70 75 80

Val Pro Pro Gly Ile Leu Glu Arg Gly Leu Leu Leu Lys Met Arg Phe

85 90 95

Ala Leu Asp His His Val Asn Leu Arg Pro Ser Lys Leu Tyr Asp Gly

100 105 110

Val Glu Ser Pro Leu Arg Asn Pro Gly Lys Ile Asp Phe Val Val Val

115 120 125

Arg Glu Gly Thr Glu Gly Ala Tyr Thr Gly Asn Gly Gly Ala Ile Arg

130 135 140

Val Gly Thr Pro His Glu Ile Ala Asn Glu Thr Ser Val Asn Thr Arg

145 150 155 160

Tyr Gly Ala Glu Arg Val Ile Arg Tyr Ala Phe Glu Leu Ala Gln Ser

165 170 175

Arg Arg Lys Lys Leu Thr Leu Val His Lys Thr Asn Val Leu Val His

180 185 190

Gly Gly Gly Leu Trp Gln Arg Thr Val Asp Glu Val Ala Lys Glu Tyr

195 200 205

Pro Glu Val Ala Val Asp Tyr Asn His Ile Asp Ala Ala Thr Ile Tyr

210 215 220

Leu Val Thr Asp Pro Ser Arg Phe Asp Val Ile Val Thr Asp Asn Leu

225 230 235 240

Phe Gly Asp Ile Leu Thr Asp Glu Ala Gly Ala Val Ser Gly Gly Ile

245 250 255

Gly Leu Ala Ala Ser Gly Asn Ile Asp Ala Thr Gly Thr Asn Pro Ser

260 265 270

Met Phe Glu Pro Val His Gly Ser Ala Pro Asp Ile Ala Gly Gln Gly

275 280 285

Ile Ala Asp Pro Thr Ala Ala Ile Leu Ser Ala Ala Met Leu Leu Arg

290 295 300

His Leu Gly Asp Glu Asp Asn Ala Val Arg Ile Glu Thr Ala Ile Ala

305 310 315 320

Ala Asp Val Ala Gly Arg Asp Asn Ser Gln Pro Ile Ser Thr Thr Glu

325 330 335

Val Gly Asp Arg Ile Val Lys Ala Leu Gln Ser

340 345

<210>53

<211>1153

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1123)

<223>RXA01240

<400>53

ttgttctcac atctacccgt atctttgttg gttttagtac ccgatagtga gttttgatcc 60

ctgcacagga aagttagcgg cgctactatg aacgatcgat atg tct gac aac act 115

Met Ser Asp Asn Thr

1 5

ctc tcc caa ttt ggc agt tac tac cac gaa ttc cga cgt gcc cat ccc 163

Leu Ser Gln Phe Gly Ser Tyr Tyr His Glu Phe Arg Arg Ala His Pro

10 15 20

atg gcc gac gtc gaa ttc ctc cta gca att gaa gaa tta ctt acg gac 211

Met Ala Asp Val Glu Phe Leu Leu Ala Ile Glu Glu Leu Leu Thr Asp

25 30 35

ggt ggt gtc acc ttc gat cgc gtc acc aca cgc atc aaa gaa tgg tca 259

Gly Gly Val Thr Phe Asp Arg Val Thr Thr Arg Ile Lys Glu Trp Ser

40 45 50

agc ctg aaa gcc aag gct cgc aag cgt cgc aac gat ggc tcg ttg atc 307

Ser Leu Lys Ala Lys Ala Arg Lys Arg Arg Asn Asp Gly Ser Leu Ile

55 60 65

tac cct gat ccg cgc aaa gac atc cac gac atg atc ggt gtt cgg atc 355

Tyr Pro Asp Pro Arg Lys Asp Ile His Asp Met Ile Gly Val Arg Ile

70 75 80 85

acc acg tac cac tcc acg gaa ata ccc gtg gcc cta aaa gtg ctc caa 403

Thr Thr Tyr His Ser Thr Glu Ile Pro Val Ala Leu Lys Val Leu Gln

90 95 100

gac tcc ttc atc gtc cac aaa tcc gta gac aaa gcc gct gaa act cgc 451

Asp Ser Phe Ile Val His Lys Ser Val Asp Lys Ala Ala Glu Thr Arg

105 110 115

atc tca ggc ggc ttt ggt tac ggc tcc cac cac ctg att ctg gaa gtc 499

Ile Ser Gly Gly Phe Gly Tyr Gly Ser His His Leu Ile Leu Glu Val

120 125 130

gat gac acc tcc gat gac ctc cag gac tac aaa ggc ctc gtc ttt gaa 547

Asp Asp Thr Ser Asp Asp Leu Gln Asp Tyr Lys Gly Leu Val Phe Glu

135 140 145

gtt cag gtg cgc acc gtg ctg caa cac gcc tgg gca gag ttc gaa cac 595

Val Gln Val Arg Thr Val Leu Gln His Ala Trp Ala Glu Phe Glu His

150 155 160 165

gat atc cgc tat aaa cgc gcc gat gtg tcc aac cca gaa gac ttc agc 643

Asp Ile Arg Tyr Lys Arg Ala Asp Val Ser Asn Pro Glu Asp Phe Ser

170 175 180

gca gaa gta gac cgc atg ttc acc ctc gct gcc gga ctc atc gaa tta 691

Ala Glu Val Asp Arg Met Phe Thr Leu Ala Ala Gly Leu Ile Glu Leu

185 190 195

gcg gac caa caa ttc gac caa atc gcc gca ctc aaa gaa acc agc cga 739

Ala Asp Gln Gln Phe Asp Gln Ile Ala Ala Leu Lys Glu Thr Ser Arg

200 205 210

gtt gct gat gaa tcc gtc gaa ctc acc gca gag aca ctt ccc ggc gtt 787

Val Ala Asp Glu Ser Val Glu Leu Thr Ala Glu Thr Leu Pro Gly Val

215 220 225

ctt gcc atg ctc att ggc aac cgc ttc ccc cgc cca cgc tcc aca aac 835

Leu Ala Met Leu Ile Gly Asn Arg Phe Pro Arg Pro Arg Ser Thr Asn

230 235 240 245

tac cgc ttc ctc gaa gac atc ctg gtg gcc aac tcc att acc tct gtg 883

Tyr Arg Phe Leu Glu Asp Ile Leu Val Ala Asn Ser Ile Thr Ser Val

250 255 260

gtg cag ctg cgc gag ctg ctc aac ccc acc gac att gaa gtg ctg ttg 931

Val Gln Leu Arg Glu Leu Leu Asn Pro Thr Asp Ile Glu Val Leu Leu

265 270 275

aaa gta atg aac tac cgc ttc cac cca ggc cag atc cgc atc atc gac 979

Lys Val Met Asn Tyr Arg Phe His Pro Gly Gln Ile Arg Ile Ile Asp

280 285 290

gat tta cta ctc aaa cga ttt ggc caa tca cac atc gat gcc acc gtc 1027

Asp Leu Leu Leu Lys Arg Phe Gly Gln Ser His Ile Asp Ala Thr Val

295 300 305

gcc acc gac tcc caa cca ctc aac gcc aaa cgc cac agg cag cta aaa 1075

Ala Thr Asp Ser Gln Pro Leu Asn Ala Lys Arg His Arg Gln Leu Lys

310 315 320 325

cgc aag cta gag ctc atg acc caa gct cat ctt gtg gaa cca cca aac 1123

Arg Lys Leu Glu Leu Met Thr Gln Ala His Leu Val Glu Pro Pro Asn

330 335 340

taaagttccc ccaaagttag ccccggctaa 1153

<210>54

<211>341

<212>PRT

<213>谷氨酸棒状杆菌

<400>54

Met Ser Asp Asn Thr Leu Ser Gln Phe Gly Ser Tyr Tyr His Glu Phe

1 5 10 15

Arg Arg Ala His Pro Met Ala Asp Val Glu Phe Leu Leu Ala Ile Glu

20 25 30

Glu Leu Leu Thr Asp Gly Gly Val Thr Phe Asp Arg Val Thr Thr Arg

35 40 45

Ile Lys Glu Trp Ser Ser Leu Lys Ala Lys Ala Arg Lys Arg Arg Asn

50 55 60

Asp Gly Ser Leu Ile Tyr Pro Asp Pro Arg Lys Asp Ile His Asp Met

65 70 75 80

Ile Gly Val Arg Ile Thr Thr Tyr His Ser Thr Glu Ile Pro Val Ala

85 90 95

Leu Lys Val Leu Gln Asp Ser Phe Ile Val His Lys Ser Val Asp Lys

100 105 110

Ala Ala Glu Thr Arg Ile Ser Gly Gly Phe Gly Tyr Gly Ser His His

115 120 125

Leu Ile Leu Glu Val Asp Asp Thr Ser Asp Asp Leu Gln Asp Tyr Lys

130 135 140

Gly Leu Val Phe Glu Val Gln Val Arg Thr Val Leu Gln His Ala Trp

145 150 155 160

Ala Glu Phe Glu His Asp Ile Arg Tyr Lys Arg Ala Asp Val Ser Asn

165 170 175

Pro Glu Asp Phe Ser Ala Glu Val Asp Arg Met Phe Thr Leu Ala Ala

180 185 190

Gly Leu Ile Glu Leu Ala Asp Gln Gln Phe Asp Gln Ile Ala Ala Leu

195 200 205

Lys Glu Thr Ser Arg Val Ala Asp Glu Ser Val Glu Leu Thr Ala Glu

210 215 220

Thr Leu Pro Gly Val Leu Ala Met Leu Ile Gly Asn Arg Phe Pro Arg

225 230 235 240

Pro Arg Ser Thr Asn Tyr Arg Phe Leu Glu Asp Ile Leu Val Ala Asn

245 250 255

Ser Ile Thr Ser Val Val Gln Leu Arg Glu Leu Leu Asn Pro Thr Asp

260 265 270

Ile Glu Val Leu Leu Lys Val Met Asn Tyr Arg Phe His Pro Gly Gln

275 280 285

Ile Arg Ile Ile Asp Asp Leu Leu Leu Lys Arg Phe Gly Gln Ser His

290 295 300

Ile Asp Ala Thr Val Ala Thr Asp Ser Gln Pro Leu Asn Ala Lys Arg

305 310 315 320

His Arg Gln Leu Lys Arg Lys Leu Glu Leu Met Thr Gln Ala His Leu

325 330 335

Val Glu Pro Pro Asn

340

<210>55

<211>1105

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1075)

<223>RXA01253

<400>55

caggctggca agaagccctc tccatggttg atcgcacagc tgatggctta gtcatcgtcg 60

tcaatggcca ggttgccgac ggcgaagacc tctcctggct ttg gga cgt ccg ctt 115

Leu Gly Arg Pro Leu

1 5

cga aga ctt cga aaa cct ctc cgt caa agc ctc cgg cga gcg cgg cac 163

Arg Arg Leu Arg Lys Pro Leu Arg Gln Ser Leu Arg Arg Ala Arg His

10 15 20

cga cct ggc agt ccg cct cac cta cgc cga aat cga cca cga act cat 211

Arg Pro Gly Ser Pro Pro His Leu Arg Arg Asn Arg Pro Arg Thr His

25 30 35

ctc caa ccc cgt cga cgc cat cgc agc ctg ccc tcc tgg ccg cat cga 259

Leu Gln Pro Arg Arg Arg His Arg Ser Leu Pro Ser Trp Pro His Arg

40 45 50

agt cct cgc caa cta cac cgc att ccg aga cct caa aaa ggc tct gga 307

Ser Pro Arg Gln Leu His Arg Ile Pro Arg Pro Gln Lys Gly Ser Gly

55 60 65

gaa agg gac cga aca ata atg acc acc ctc aac atc ggc ctc atc ctc 355

Glu Arg Asp Arg Thr Ile Met Thr Thr Leu Asn Ile Gly Leu Ile Leu

70 75 80 85

ccc gac gtc ctc gga act tac ggc gac gac ggc aac gca cta gtc ctg 403

Pro Asp Val Leu Gly Thr Tyr Gly Asp Asp Gly Asn Ala Leu Val Leu

90 95 100

cgc caa cgc gca cgc atg cgt ggc att aat gct gaa atc cag cgc gtc 451

Arg Gln Arg Ala Arg Met Arg Gly Ile Asn Ala Glu Ile Gln Arg Val

105 110 115

acc ctc gac gac gcc gtc cct tcc acc ctt gat ctc tac tgc ctc ggc 499

Thr Leu Asp Asp Ala Val Pro Ser Thr Leu Asp Leu Tyr Cys Leu Gly

120 125 130

ggc ggc gag gac acc gca cag atc ctt gcc acc gaa cac ctc acc aaa 547

Gly Gly Glu Asp Thr Ala Gln Ile Leu Ala Thr Glu His Leu Thr Lys

135 140 145

gac ggc ggc ctc caa acc gca gcc gcc gca ggc cgc ccc atc ttc gca 595

Asp Gly Gly Leu Gln Thr Ala Ala Ala Ala Gly Arg Pro Ile Phe Ala

150 155 160 165

gtc tgc gca ggt ctc cag gta ctc ggc gac tcc ttc cgc gcc gcc ggc 643

Val Cys Ala Gly Leu Gln Val Leu Gly Asp Ser Phe Arg Ala Ala Gly

170 175 180

cgt gtc atc gac ggc ctt ggg ctt atc gac gcc acc acc gtc tct tta 691

Arg Val Ile Asp Gly Leu Gly Leu Ile Asp Ala Thr Thr Val Ser Leu

185 190 195

caa aaa cgc gcc atc gga gaa gtc gaa acg aca cca acc cgc gcc gga 739

Gln Lys Arg Ala Ile Gly Glu Val Glu Thr Thr Pro Thr Arg Ala Gly

200 205 210

ttc acc gcc gag ctg acc gaa cga ctc acc ggc ttc gaa aac cac atg 787

Phe Thr Ala Glu Leu Thr Glu Arg Leu Thr Gly Phe Glu Asn His Met

215 220 225

ggc gcc acc ctg ctc ggc ccc gac gcc gaa cca ctc ggc cga gtc gtc 835

Gly Ala Thr Leu Leu Gly Pro Asp Ala Glu Pro Leu Gly Arg Val Val

230 235 240 245

cgc ggc gaa ggc aac acc gat gtc tgg gca gcc tcc gaa aac acc gac 883

Arg Gly Glu Gly Asn Thr Asp Val Trp Ala Ala Ser Glu Asn Thr Asp

250 255 260

gac caa cgc caa caa ttc gcc gaa ggc gcc gtc caa ggc agc atc atc 931

Asp Gln Arg Gln Gln Phe Ala Glu Gly Ala Val Gln Gly Ser Ile Ile

265 270 275

gcc acc tac atg cac ggc ccc gca ctc gcc cga aac ccc caa ctc gcc 979

Ala Thr Tyr Met His Gly Pro Ala Leu Ala Arg Asn Pro Gln Leu Ala

280 285 290

gac ctc atg ctc gca aaa gca atg ggt gtc gcg ctg aaa gac ctg gag 1027

Asp Leu Met Leu Ala Lys Ala Met Gly Val Ala Leu Lys Asp Leu Glu

295 300 305

cct ttg gac atc gac gtc atc gac cgc ctc cgc gcc gaa cgc ctg gcg 1075

Pro Leu Asp Ile Asp Val Ile Asp Arg Leu Arg Ala Glu Arg Leu Ala

310 315 320 325

tagccccttc taaaccgggt ctactcgaaa 1105

<210>56

<211>325

<212>PRT

<213>谷氨酸棒状杆菌

<400>56

Leu Gly Arg Pro Leu Arg Arg Leu Arg Lys Pro Leu Arg Gln Ser Leu

1 5 10 15

Arg Arg Ala Arg His Arg Pro Gly Ser Pro Pro His Leu Arg Arg Asn

20 25 30

Arg Pro Arg Thr His Leu Gln Pro Arg Arg Arg His Arg Ser Leu Pro

35 40 45

Ser Trp Pro His Arg Ser Pro Arg Gln Leu His Arg Ile Pro Arg Pro

50 55 60

Gln Lys Gly Ser Gly Glu Arg Asp Arg Thr Ile Met Thr Thr Leu Asn

65 70 75 80

Ile Gly Leu Ile Leu Pro Asp Val Leu Gly Thr Tyr Gly Asp Asp Gly

85 90 95

Asn Ala Leu Val Leu Arg Gln Arg Ala Arg Met Arg Gly Ile Asn Ala

100 105 110

Glu Ile Gln Arg Val Thr Leu Asp Asp Ala Val Pro Ser Thr Leu Asp

115 120 125

Leu Tyr Cys Leu Gly Gly Gly Glu Asp Thr Ala Gln Ile Leu Ala Thr

130 135 140

Glu His Leu Thr Lys Asp Gly Gly Leu Gln Thr Ala Ala Ala Ala Gly

145 150 155 160

Arg Pro Ile Phe Ala Val Cys Ala Gly Leu Gln Val Leu Gly Asp Ser

165 170 175

Phe Arg Ala Ala Gly Arg Val Ile Asp Gly Leu Gly Leu Ile Asp Ala

180 185 190

Thr Thr Val Ser Leu Gln Lys Arg Ala Ile Gly Glu Val Glu Thr Thr

195 200 205

Pro Thr Arg Ala Gly Phe Thr Ala Glu Leu Thr Glu Arg Leu Thr Gly

210 215 220

Phe Glu Asn His Met Gly Ala Thr Leu Leu Gly Pro Asp Ala Glu Pro

225 230 235 240

Leu Gly Arg Val Val Arg Gly Glu Gly Asn Thr Asp Val Trp Ala Ala

245 250 255

Ser Glu Asn Thr Asp Asp Gln Arg Gln Gln Phe Ala Glu Gly Ala Val

260 265 270

Gln Gly Ser Ile Ile Ala Thr Tyr Met His Gly Pro Ala Leu Ala Arg

275 280 285

Asn Pro Gln Leu Ala Asp Leu Met Leu Ala Lys Ala Met Gly Val Ala

290 295 300

Leu Lys Asp Leu Glu Pro Leu Asp Ile Asp Val Ile Asp Arg Leu Arg

305 310 315 320

Ala Glu Arg Leu Ala

325

<210>57

<211>1132

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1102)

<223>RXA01321

<400>57

ggacacagat cgtgccgact cccagctcaa ttgcggaatc tataaatgcg gccagttcgg 60

agttggttac ttctggtccg aggagggtgt agtcaaggat ttg ggc cat ggt gga 115

Leu Gly His Gly Gly

1 5

gcg gga aat cgt cat atc cat acc cta ctt aga cct gac tta gtg tgg 163

Ala Gly Asn Arg His Ile His Thr Leu Leu Arg Pro Asp Leu Val Trp

10 15 20

gaa aat ttc cag ggt aga atg caa cga atg acc ccg agt tct cct gaa 211

Glu Asn Phe Gln Gly Arg Met Gln Arg Met Thr Pro Ser Ser Pro Glu

25 30 35

gtt cgt aat cgt ccg agc gct gcg cct gaa gag cgt cag ttt gtg ctc 259

Val Arg Asn Arg Pro Ser Ala Ala Pro Glu Glu Arg Gln Phe Val Leu

40 45 50

act ttt ggc tgc cct gac tcc act gga att gtg gcg aag ttg tcg tcg 307

Thr Phe Gly Cys Pro Asp Ser Thr Gly Ile Val Ala Lys Leu Ser Ser

55 60 65

ttc cta gct gag cgt ggg ggt tgg att act gag gct gga tat ttc acg 355

Phe Leu Ala Glu Arg Gly Gly Trp Ile Thr Glu Ala Gly Tyr Phe Thr

70 75 80 85

gat cct gat tcg aat tgg ttc ttt act cgt cag gcg att cgc gct gag 403

Asp Pro Asp Ser Asn Trp Phe Phe Thr Arg Gln Ala Ile Arg Ala Glu

90 95 100

tcg att gat acc acg att gag cag ttg cgg gag gag ttc gct ccg ctt 451

Ser Ile Asp Thr Thr Ile Glu Gln Leu Arg Glu Glu Phe Ala Pro Leu

105 110 115

gcg gag gag ttc ggc ccg agg gct aag tgg agt ttc act gac act gcg 499

Ala Glu Glu Phe Gly Pro Arg Ala Lys Trp Ser Phe Thr Asp Thr Ala

120 125 130

cag gtg aag aag gct gtg ttg ttg gtg tct aag gag ggc cac tgc ttg 547

Gln Val Lys Lys Ala Val Leu Leu Val Ser Lys Glu Gly His Cys Leu

135 140 145

cac gat ttg tta ggt cgt gtg gct gag aat gat tat ccg atg gaa gtt 595

His Asp Leu Leu Gly Arg Val Ala Glu Asn Asp Tyr Pro Met Glu Val

150 155 160 165

gtt gcg gtt gtg ggt aac cat gag aac ttg cgt tat att gcg gag aac 643

Val Ala Val Val Gly Asn His Glu Asn Leu Arg Tyr Ile Ala Glu Asn

170 175 180

cat aat gtt ccg ttt ttc cat gtg ccg ttt cct aag gat gcg gtt ggt 691

His Asn Val Pro Phe Phe His Val Pro Phe Pro Lys Asp Ala Val Gly

185 190 195

aag cgg aag gcg ttt gac cag gtc gct gag att gtg aat ggt tat gat 739

Lys Arg Lys Ala Phe Asp Gln Val Ala Glu Ile Val Asn Gly Tyr Asp

200 205 210

ccg gat gcg att gtt ttg gct cgt ttt atg cag att ttg ccg ccg gat 787

Pro Asp Ala Ile Val Leu Ala Arg Phe Met Gln Ile Leu Pro Pro Asp

215 220 225

ttg tgt gag atg tgg gct ggt cgt gtg ttg aat att cat cac agt ttc 835

Leu Cys Glu Met Trp Ala Gly Arg Val Leu Asn Ile His His Ser Phe

230 235 240 245

ttg ccg tcg ttt atg ggt gcg cgc ccg tat cat cag gcg tat agc cgt 883

Leu Pro Ser Phe Met Gly Ala Arg Pro Tyr His Gln Ala Tyr Ser Arg

250 255 260

ggt gtg aag ttg att ggt gcg acc tgc cat tat gcg act ggg gat ctg 931

Gly Val Lys Leu Ile Gly Ala Thr Cys His Tyr Ala Thr Gly Asp Leu

265 270 275

gat gat ggt ccg atc att gag cag gat gtt att cgt gtg acg cat aag 979

Asp Asp Gly Pro Ile Ile Glu Gln Asp Val Ile Arg Val Thr His Lys

280 285 290

gat acg ccg act gag atg cag cgt ttg ggc cgc gat gcg gag aag cag 1027

Asp Thr Pro Thr Glu Met Gln Arg Leu Gly Arg Asp Ala Glu Lys Gln

295 300 305

gtg ctg gct cgc ggt ttg cgt ttc cac ttg gag gac cgg gtg ctg gtt 1075

Val Leu Ala Arg Gly Leu Arg Phe His Leu Glu Asp Arg Val Leu Val

310 315 320 325

tac ggt aac cgc acg gtt gtc ttt gat taaggctttt tgcttttcga 1122

Tyr Gly Asn Arg Thr Val Val Phe Asp

330

cgcgacccta 1132

<210>58

<211>334

<212>PRT

<213>谷氨酸棒状杆菌

<400>58

Leu Gly His Gly Gly Ala Gly Asn Arg His Ile His Thr Leu Leu Arg

1 5 10 15

Pro Asp Leu Val Trp Glu Asn Phe Gln Gly Arg Met Gln Arg Met Thr

20 25 30

Pro Ser Ser Pro Glu Val Arg Asn Arg Pro Ser Ala Ala Pro Glu Glu

35 40 45

Arg Gln Phe Val Leu Thr Phe Gly Cys Pro Asp Ser Thr Gly Ile Val

50 55 60

Ala Lys Leu Ser Ser Phe Leu Ala Glu Arg Gly Gly Trp Ile Thr Glu

65 70 75 80

Ala Gly Tyr Phe Thr Asp Pro Asp Ser Asn Trp Phe Phe Thr Arg Gln

85 90 95

Ala Ile Arg Ala Glu Ser Ile Asp Thr Thr Ile Glu Gln Leu Arg Glu

100 105 110

Glu Phe Ala Pro Leu Ala Glu Glu Phe Gly Pro Arg Ala Lys Trp Ser

115 120 125

Phe Thr Asp Thr Ala Gln Val Lys Lys Ala Val Leu Leu Val Ser Lys

130 135 140

Glu Gly His Cys Leu His Asp Leu Leu Gly Arg Val Ala Glu Asn Asp

145 150 155 160

Tyr Pro Met Glu Val Val Ala Val Val Gly Asn His Glu Asn Leu Arg

165 170 175

Tyr Ile Ala Glu Asn His Asn Val Pro Phe Phe His Val Pro Phe Pro

180 185 190

Lys Asp Ala Val Gly Lys Arg Lys Ala Phe Asp Gln Val Ala Glu Ile

195 200 205

Val Asn Gly Tyr Asp Pro Asp Ala Ile Val Leu Ala Arg Phe Met Gln

210 215 220

Ile Leu Pro Pro Asp Leu Cys Glu Met Trp Ala Gly Arg Val Leu Asn

225 230 235 240

Ile His His Ser Phe Leu Pro Ser Phe Met Gly Ala Arg Pro Tyr His

245 250 255

Gln Ala Tyr Ser Arg Gly Val Lys Leu Ile Gly Ala Thr Cys His Tyr

260 265 270

Ala Thr Gly Asp Leu Asp Asp Gly Pro Ile Ile Glu Gln Asp Val Ile

275 280 285

Arg Val Thr His Lys Asp Thr Pro Thr Glu Met Gln Arg Leu Gly Arg

290 295 300

Asp Ala Glu Lys Gln Val Leu Ala Arg Gly Leu Arg Phe His Leu Glu

305 310 315 320

Asp Arg Val Leu Val Tyr Gly Asn Arg Thr Val Val Phe Asp

325 330

<210>59

<211>1243

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1213)

<223>RXA01381

<400>59

gcgatggccg aatccatgtt ccacgccgtt gaagccggaa gattagccgc ccaagcaggc 60

cggatcccgc aacgccaaca tgcggtggcc tcatcaagtt ttg aag gtt tgg cat 115

Leu Lys Val Trp His

1 5

cct ggg cgg agc agg tgc ttt aac atg gcg cag ctt ccc aca tct gag 163

Pro Gly Arg Ser Arg Cys Phe Asn Met Ala Gln Leu Pro Thr Ser Glu

10 15 20

ctt cac cgc acg gcc cgc caa ctc gcg ctc ccc gga tat ggt att gag 211

Leu His Arg Thr Ala Arg Gln Leu Ala Leu Pro Gly Tyr Gly Ile Glu

25 30 35

cag cag gaa cga ctt ttc aac gcc cac gtt cta gtc atc ggc gca ggc 259

Gln Gln Glu Arg Leu Phe Asn Ala His Val Leu Val Ile Gly Ala Gly

40 45 50

ggt ctc ggc tgc cca gtc atg cag tcg ctg gct tcc gca ggc gtt gga 307

Gly Leu Gly Cys Pro Val Met Gln Ser Leu Ala Ser Ala Gly Val Gly

55 60 65

acc atc acg gtc atc gat gac gac acc gtc gac att tcc aac att cac 355

Thr Ile Thr Val Ile Asp Asp Asp Thr Val Asp Ile Ser Asn Ile His

70 75 80 85

cgc caa atc ctc ttc ggc gca agc gat gtc ggt cga ccc aag gtc gag 403

Arg Gln Ile Leu Phe Gly Ala Ser Asp Val Gly Arg Pro Lys Val Glu

90 95 100

gtt gcc gcc gag cgc ctc aaa gaa ctc caa cca gac atc acc gtc aac 451

Val Ala Ala Glu Arg Leu Lys Glu Leu Gln Pro Asp Ile Thr Val Asn

105 110 115

gcg ttg cac gaa cgg atc act cca gaa aac gcc tgc gag ctg ctc aat 499

Ala Leu His Glu Arg Ile Thr Pro Glu Asn Ala Cys Glu Leu Leu Asn

120 125 130

tcc gtg gac ctc gtc tta gac ggc tcc gat tct ttc tcc aca aaa tac 547

Ser Val Asp Leu Val Leu Asp Gly Ser Asp Ser Phe Ser Thr Lys Tyr

135 140 145

tta gtg tct gat gcc gcc gaa atc acc gga act ccc ctc atc tgg gca 595

Leu Val Ser Asp Ala Ala Glu Ile Thr Gly Thr Pro Leu Ile Trp Ala

150 155 160 165

acg gta ctg cgc ttt cac ggc gaa ctg gca ctc ttc aac tct ggc ccc 643

Thr Val Leu Arg Phe His Gly Glu Leu Ala Leu Phe Asn Ser Gly Pro

170 175 180

gac cac cgc gga gtc ggc ctg cgc gac gtc ttc ccc gaa caa ccc tcc 691

Asp His Arg Gly Val Gly Leu Arg Asp Val Phe Pro Glu Gln Pro Ser

185 190 195

gcc gat ttc gtc cct gac tgc gcc acc gct ggt gtt ctt ggc gcc acc 739

Ala Asp Phe Val Pro Asp Cys Ala Thr Ala Gly Val Leu Gly Ala Thr

200 205 210

aca gcc acc atc ggc gca ctc atg gcc act cac gcc atc gga ttt ctc 787

Thr Ala Thr Ile Gly Ala Leu Met Ala Thr His Ala Ile Gly Phe Leu

215 220 225

aca gaa atc ggc gac gtc caa cca ggc aca atc ctc tcc tac gac gca 835

Thr Glu Ile Gly Asp Val Gln Pro Gly Thr Ile Leu Ser Tyr Asp Ala

230 235 240 245

ttc ccc gcc gcc acg cgc agc ttc cgc gtc tcc gcc gac ccg gcg cgc 883

Phe Pro Ala Ala Thr Arg Ser Phe Arg Val Ser Ala Asp Pro Ala Arg

250 255 260

cca ctg gtc acc cgc ctc cgc gcc tcc tac gag gca gcg cgc acc gat 931

Pro Leu Val Thr Arg Leu Arg Ala Ser Tyr Glu Ala Ala Arg Thr Asp

265 270 275

aca act tcg ctt atc gac gcc acc ctc aac ggc tcc ctc acc gcc ctc 979

Thr Thr Ser Leu Ile Asp Ala Thr Leu Asn Gly Ser Leu Thr Ala Leu

280 285 290

gat atc cga gag cca cat gaa gtt ctg ctc aaa gac ctc ccc gag ggc 1027

Asp Ile Arg Glu Pro His Glu Val Leu Leu Lys Asp Leu Pro Glu Gly

295 300 305

gca acg tca ctg aag ctc ccc tta agc cag atc acc tcg gac agc gac 1075

Ala Thr Ser Leu Lys Leu Pro Leu Ser Gln Ile Thr Ser Asp Ser Asp

310 315 320 325

att tta gag gca ctg tct gga atc gac ggc gac att ttg gtc tac tgc 1123

Ile Leu Glu Ala Leu Ser Gly Ile Asp Gly Asp Ile Leu Val Tyr Cys

330 335 340

gct tcg gga atc cgc agt tcc gac ttc atc gac aac tac tcc cac ctc 1171

Ala Ser Gly Ile Arg Ser Ser Asp Phe Ile Asp Asn Tyr Ser His Leu

345 350 355

ggc cac aaa ttt gtg aat ctt ccc ggt ggg gtc aac gcg ctg 1213

Gly His Lys Phe Val Asn Leu Pro Gly Gly Val Asn Ala Leu

360 365 370

tagctgtcaa tttaagaggc cagaattctg 1243

<210>60

<211>371

<212>PRT

<213>谷氨酸棒状杆菌

<400>60

Leu Lys Val Trp His Pro Gly Arg Ser Arg Cys Phe Asn Met Ala Gln

1 5 10 15

Leu Pro Thr Ser Glu Leu His Arg Thr Ala Arg Gln Leu Ala Leu Pro

20 25 30

Gly Tyr Gly Ile Glu Gln Gln Glu Arg Leu Phe Asn Ala His Val Leu

35 40 45

Val Ile Gly Ala Gly Gly Leu Gly Cys Pro Val Met Gln Ser Leu Ala

50 55 60

Ser Ala Gly Val Gly Thr Ile Thr Val Ile Asp Asp Asp Thr Val Asp

65 70 75 80

Ile Ser Asn Ile His Arg Gln Ile Leu Phe Gly Ala Ser Asp Val Gly

85 90 95

Arg Pro Lys Val Glu Val Ala Ala Glu Arg Leu Lys Glu Leu Gln Pro

100 105 110

Asp Ile Thr Val Asn Ala Leu His Glu Arg Ile Thr Pro Glu Asn Ala

115 120 125

Cys Glu Leu Leu Asn Ser Val Asp Leu Val Leu Asp Gly Ser Asp Ser

130 135 140

Phe Ser Thr Lys Tyr Leu Val Ser Asp Ala Ala Glu Ile Thr Gly Thr

145 150 155 160

Pro Leu Ile Trp Ala Thr Val Leu Arg Phe His Gly Glu Leu Ala Leu

165 170 175

Phe Asn Ser Gly Pro Asp His Arg Gly Val Gly Leu Arg Asp Val Phe

180 185 190

Pro Glu Gln Pro Ser Ala Asp Phe Val Pro Asp Cys Ala Thr Ala Gly

195 200 205

Val Leu Gly Ala Thr Thr Ala Thr Ile Gly Ala Leu Met Ala Thr His

210 215 220

Ala Ile Gly Phe Leu Thr Glu Ile Gly Asp Val Gln Pro Gly Thr Ile

225 230 235 240

Leu Ser Tyr Asp Ala Phe Pro Ala Ala Thr Arg Ser Phe Arg Val Ser

245 250 255

Ala Asp Pro Ala Arg Pro Leu Val Thr Arg Leu Arg Ala Ser Tyr Glu

260 265 270

Ala Ala Arg Thr Asp Thr Thr Ser Leu Ile Asp Ala Thr Leu Asn Gly

275 280 285

Ser Leu Thr Ala Leu Asp Ile Arg Glu Pro His Glu Val Leu Leu Lys

290 295 300

Asp Leu Pro Glu Gly Ala Thr Ser Leu Lys Leu Pro Leu Ser Gln Ile

305 310 315 320

Thr Ser Asp Ser Asp Ile Leu Glu Ala Leu Ser Gly Ile Asp Gly Asp

325 330 335

Ile Leu Val Tyr Cys Ala Ser Gly Ile Arg Ser Ser Asp Phe Ile Asp

340 345 350

Asn Tyr Ser His Leu Gly His Lys Phe Val Asn Leu Pro Gly Gly Val

355 360 365

Asn Ala Leu

370

<210>61

<211>3457

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3427)

<223>RXA01434

<400>61

acgggatcgc ccaaaccgcc accaacccac taacgtatcg catagttatt ttcgccgtgc 60

tcgccgttaa ggcgcgcact cgtacatgaa tggtcaacaa gtg agt tct tcg ctt 115

Val Ser Ser Ser Leu

1 5

tcg aat aat tcg gag cag tcc ggc ctg cgt ggc agg atc gtt gct cca 163

Ser Asn Asn Ser Glu Gln Ser Gly Leu Arg Gly Arg Ile Val Ala Pro

10 15 20

gca ccg ccg gcg cct gtg ccc gag gcg cgc aag aag gct gtc gca cgc 211

Ala Pro Pro Ala Pro Val Pro Glu Ala Arg Lys Lys Ala Val Ala Arg

25 30 35

acg gat ggt gat cgc tcg agt ttg aaa aac tcg cct acg gca tcc gcc 259

Thr Asp Gly Asp Arg Ser Ser Leu Lys Asn Ser Pro Thr Ala Ser Ala

40 45 50

acc cag gca gcc cag acg cgc ccg gca gaa ccg gaa cca aaa aag cac 307

Thr Gln Ala Ala Gln Thr Arg Pro Ala Glu Pro Glu Pro Lys Lys His

55 60 65

acc tcc gat tct gat gtg gtg cgc tcg act ggc tcc atg gca ata gcc 355

Thr Ser Asp Ser Asp Val Val Arg Ser Thr Gly Ser Met Ala Ile Ala

70 75 80 85

acg ctg ctg agt cgt atc acc ggt ttc ctg cgc acc gtg atg att ggt 403

Thr Leu Leu Ser Arg Ile Thr Gly Phe Leu Arg Thr Val Met Ile Gly

90 95 100

gcg gcg ctg tcg ccg gcc atc gct tcg gcg ttc aac act gcc aac acg 451

Ala Ala Leu Ser Pro Ala Ile Ala Ser Ala Phe Asn Thr Ala Asn Thr

105 110 115

ctg ccc aac ctg atc act gaa atc gtg ttg ggt gcg gtg ctg aca tcg 499

Leu Pro Asn Leu Ile Thr Glu Ile Val Leu Gly Ala Val Leu Thr Ser

120 125 130

ctg gtt att ccg gtc ctt acc cgc gcg gaa aaa gaa gac gcc gac ggc 547

Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys Glu Asp Ala Asp Gly

135 140 145

ggt tcc ggg ttc ttc agg cgg ctg ctc acc ctg tcg gtg acg ctg ctg 595

Gly Ser Gly Phe Phe Arg Arg Leu Leu Thr Leu Ser Val Thr Leu Leu

150 155 160 165

ggt ggt gtc acc atc ctg tcg att atc ggc gcg ccg ctg ctg aca cgg 643

Gly Gly Val Thr Ile Leu Ser Ile Ile Gly Ala Pro Leu Leu Thr Arg

170 175 180

atg atg ctg tcc tct gag gga caa gtc aac gtg gtc atg tcc acg gcc 691

Met Met Leu Ser Ser Glu Gly Gln Val Asn Val Val Met Ser Thr Ala

185 190 195

ttt gcg tat tgg ctg ctg cca cag att ttc ttc tac ggc ctg ttt gcc 739

Phe Ala Tyr Trp Leu Leu Pro Gln Ile Phe Phe Tyr Gly Leu Phe Ala

200 205 210

ctg ttc atg gct gtg ttg aac acc cgt gaa gtg ttc aaa ccc ggc gcg 787

Leu Phe Met Ala Val Leu Asn Thr Arg Glu Val Phe Lys Pro Gly Ala

215 220 225

tgg gca cct gtt gtc aac aat gtg atc acc ttg acc gtg ctg ggc gtg 835

Trp Ala Pro Val Val Asn Asn Val Ile Thr Leu Thr Val Leu Gly Val

230 235 240 245

tac atg gtg ctg cct gcg cgt ttg cac ccg cat gag cag gtg ggc att 883

Tyr Met Val Leu Pro Ala Arg Leu His Pro His Glu Gln Val Gly Ile

250 255 260

ttt gat ccg cag atc att ttc ctc ggc gtg ggc acc acc ctt ggt gtg 931

Phe Asp Pro Gln Ile Ile Phe Leu Gly Val Gly Thr Thr Leu Gly Val

265 270 275

gtt gca cag tgt cta atc atg att ccg tac ctg cgt cgc gcg ggc att 979

Val Ala Gln Cys Leu Ile Met Ile Pro Tyr Leu Arg Arg Ala Gly Ile

280 285 290

gat atg cgc cct ctg tgg ggt atc gat gcg cgt ttg aag caa ttc ggt 1027

Asp Met Arg Pro Leu Trp Gly Ile Asp Ala Arg Leu Lys Gln Phe Gly

295 300 305

ggc atg gcg atg gcg atc atc gtg tac gtg gca atc tcc cag ttc ggt 1075

Gly Met Ala Met Ala Ile Ile Val Tyr Val Ala Ile Ser Gln Phe Gly

310 315 320 325

tac atc atc acc act cgc att gcg tcg att gca gac gat gct gcg ccg 1123

Tyr Ile Ile Thr Thr Arg Ile Ala Ser Ile Ala Asp Asp Ala Ala Pro

330 335 340

ttt att tat cag cag cac tgg atg ttg ctg caa gtt cct tat ggc atc 1171

Phe Ile Tyr Gln Gln His Trp Met Leu Leu Gln Val Pro Tyr Gly Ile

345 350 355

atc ggc gtc acc ttg ctc acc gcg att atg ccg cga ctg tcc cgc aac 1219

Ile Gly Val Thr Leu Leu Thr Ala Ile Met Pro Arg Leu Ser Arg Asn

360 365 370

gcg gca gac ggc gat gat agg gca gta gtc tct gac ctt cag ttg ggt 1267

Ala Ala Asp Gly Asp Asp Arg Ala Val Val Ser Asp Leu Gln Leu Gly

375 380 385

tcc aag cta acc ttc atc gca ctg atc ccc atc gtg gtg ttc ttc acc 1315

Ser Lys Leu Thr Phe Ile Ala Leu Ile Pro Ile Val Val Phe Phe Thr

390 395 400 405

gcc ttc ggt gtc cct att gcc aat ggc ctt ttt gcc tac ggc caa ttc 1363

Ala Phe Gly Val Pro Ile Ala Asn Gly Leu Phe Ala Tyr Gly Gln Phe

410 415 420

gat gcc aac gcc gcc aac atc ctt ggt tgg act ctg agc ttc tct gct 1411

Asp Ala Asn Ala Ala Asn Ile Leu Gly Trp Thr Leu Ser Phe Ser Ala

425 430 435

ttc acg ctg att cct tac gct ttg gtg ctg cta cat ctg cgt gtg ttt 1459

Phe Thr Leu Ile Pro Tyr Ala Leu Val Leu Leu His Leu Arg Val Phe

440 445 450

tat gcg cgt gaa gag gtc tgg acc cca acc ttc atc atc gcc ggc atc 1507

Tyr Ala Arg Glu Glu Val Trp Thr Pro Thr Phe Ile Ile Ala Gly Ile

455 460 465

acc gcc acc aag gtc gtg ctt tcc ctg ttg gca ccg ctg ctg tcg agc 1555

Thr Ala Thr Lys Val Val Leu Ser Leu Leu Ala Pro Leu Leu Ser Ser

470 475 480 485

tcc ccg gag cgt gtg gtg gtg ctt ctt ggt gcg gcc aac ggt ttc agt 1603

Ser Pro Glu Arg Val Val Val Leu Leu Gly Ala Ala Asn Gly Phe Ser

490 495 500

ttc atc acc ggc gcg gtc atc ggc gcg tat ctg ttg cgc aac aaa ctc 1651

Phe Ile Thr Gly Ala Val Ile Gly Ala Tyr Leu Leu Arg Asn Lys Leu

505 510 515

ggc ctg ttg ggt atg cgc tct ttg gct aaa acc tcc ctg tgg gcg ttg 1699

Gly Leu Leu Gly Met Arg Ser Leu Ala Lys Thr Ser Leu Trp Ala Leu

520 525 530

ggc tct gcg gcg gtt ggt gca gca gca gca tgg gcg ttg ggg tgg ctg 1747

Gly Ser Ala Ala Val Gly Ala Ala Ala Ala Trp Ala Leu Gly Trp Leu

535 540 545

att caa gcc gtc gtg ggc gat ttc ttg ctg ggc act cta agc tcc gta 1795

Ile Gln Ala Val Val Gly Asp Phe Leu Leu Gly Thr Leu Ser Ser Val

550 555 560 565

ggc tac ttg ttg aac ctg gct gtg ttg ggt gtc ttc ttc atc ttc gtc 1843

Gly Tyr Leu Leu Asn Leu Ala Val Leu Gly Val Phe Phe Ile Phe Val

570 575 580

acc ggc atc gtg ttg tca cgt tct ggt ttg ccg gag gtc caa aac ttg 1891

Thr Gly Ile Val Leu Ser Arg Ser Gly Leu Pro Glu Val Gln Asn Leu

585 590 595

ggc cag gca ctg acc cgc atc cca ggt ttg agc cgg ttt att cgc ccg 1939

Gly Gln Ala Leu Thr Arg Ile Pro Gly Leu Ser Arg Phe Ile Arg Pro

600 605 610

aat acc aag atc tct ttg gat gtc ggc gaa gtc tcc gag cag gat ttc 1987

Asn Thr Lys Ile Ser Leu Asp Val Gly Glu Val Ser Glu Gln Asp Phe

615 620 625

tcc acc cag ctg gtc gcg cca agc gag ttc gca gca acc cct gtg ccg 2035

Ser Thr Gln Leu Val Ala Pro Ser Glu Phe Ala Ala Thr Pro Val Pro

630 635 640 645

cca ccg atg tcc gcc ggt att gtc cgc gga cct cgc ctg gtt ccc ggc 2083

Pro Pro Met Ser Ala Gly Ile Val Arg Gly Pro Arg Leu Val Pro Gly

650 655 660

gcc cca gtc ggc gac ggt cgt ttc cgt ctg ctc gcc gat cac ggc ggc 2131

Ala Pro Val Gly Asp Gly Arg Phe Arg Leu Leu Ala Asp His Gly Gly

665 670 675

gtc caa ggc gcg cgt ttc tgg cag gcc cgc gag atc gcc acc ggc aag 2179

Val Gln Gly Ala Arg Phe Trp Gln Ala Arg Glu Ile Ala Thr Gly Lys

680 685 690

gaa gtc gcg ctg atc ttt gtg gat act tcc ggc aac gcc cca ttt gcg 2227

Glu Val Ala Leu Ile Phe Val Asp Thr Ser Gly Asn Ala Pro Phe Ala

695 700 705

cca ctg tct tcg gca gcc gca gcg ggc atc gcc tac gag gtg cag cgc 2275

Pro Leu Ser Ser Ala Ala Ala Ala Gly Ile Ala Tyr Glu Val Gln Arg

710 715 720 725

cgc acc aag aag ctg gcc agc ttg ggc agc ttg gcg gta gcc ccc aac 2323

Arg Thr Lys Lys Leu Ala Ser Leu Gly Ser Leu Ala Val Ala Pro Asn

730 735 740

atc cac tcc gag gcg tac cgc aac ggt tgc ctc att gtg gcc gat tgg 2371

Ile His Ser Glu Ala Tyr Arg Asn Gly Cys Leu Ile Val Ala Asp Trp

745 750 755

gtg cct ggc tcc agc ttg agc gcc gtc gcg gaa tcc ggt gcc gat ccc 2419

Val Pro Gly Ser Ser Leu Ser Ala Val Ala Glu Ser Gly Ala Asp Pro

760 765 770

cgc gcc gcc gcg ttc gcg ctc gcg gaa cta act gaa acc atc ggc gag 2467

Arg Ala Ala Ala Phe Ala Leu Ala Glu Leu Thr Glu Thr Ile Gly Glu

775 780 785

gcc cac gag atg ggt atc ccg gcc ggc ttg gac aac aag tgc cgc atc 2515

Ala His Glu Met Gly Ile Pro Ala Gly Leu Asp Asn Lys Cys Arg Ile

790 795 800 805

cgc atc aac acc gac ggc cat gcc gtc ctc gcc ttc ccg gcg att ttg 2563

Arg Ile Asn Thr Asp Gly His Ala Val Leu Ala Phe Pro Ala Ile Leu

810 815 820

ccc gat gcc tca gag ctc cgc gac gcc aag tcc ctg gcc tcg gcc gcc 2611

Pro Asp Ala Ser Glu Leu Arg Asp Ala Lys Ser Leu Ala Ser Ala Ala

825 830 835

gag atg ctt atc gac gcg acc ctc gct ccc agc gac gtc aag gca atg 2659

Glu Met Leu Ile Asp Ala Thr Leu Ala Pro Ser Asp Val Lys Ala Met

840 845 850

gtc act gaa gcc cag ggg cta gct aca gaa gac aat ccc gat tac gca 2707

Val Thr Glu Ala Gln Gly Leu Ala Thr Glu Asp Asn Pro Asp Tyr Ala

855 860 865

tca ctt gcc atg gcg atg cgc acc tgc gga ctg ttc acc gag gaa cca 2755

Ser Leu Ala Met Ala Met Arg Thr Cys Gly Leu Phe Thr Glu Glu Pro

870 875 880 885

acc cac ctt gtg gtg aag aag gaa aag aca cca aag cct gcg aca cgt 2803

Thr His Leu Val Val Lys Lys Glu Lys Thr Pro Lys Pro Ala Thr Arg

890 895 900

gat ggt ttc ggt gcc tcc gac tac acc gtc aag ggc atg gca gcc atc 2851

Asp Gly Phe Gly Ala Ser Asp Tyr Thr Val Lys Gly Met Ala Ala Ile

905 910 915

gcc gct gtg gtg atc atc ttg gtt tcc ctg gtg gcc gcc ggt acc gcg 2899

Ala Ala Val Val Ile Ile Leu Val Ser Leu Val Ala Ala Gly Thr Ala

920 925 930

ttc ctc acc agc ttc ttc ggc agc agc acc aac gaa caa tcc ccg ttg 2947

Phe Leu Thr Ser Phe Phe Gly Ser Ser Thr Asn Glu Gln Ser Pro Leu

935 940 945

gcc tct gtt gaa gcc acc acc tct gca aca cca gaa cct gtg ggg cca 2995

Ala Ser Val Glu Ala Thr Thr Ser Ala Thr Pro Glu Pro Val Gly Pro

950 955 960 965

ccg gtc tac ctg gat ctg gat caa gcc cgc acg tgg gat gac ggt gca 3043

Pro Val Tyr Leu Asp Leu Asp Gln Ala Arg Thr Trp Asp Asp Gly Ala

970 975 980

gga aca gat gtc acc gac gtc acc gac ggc aac acc tcc acc gca tgg 3091

Gly Thr Asp Val Thr Asp Val Thr Asp Gly Asn Thr Ser Thr Ala Trp

985 990 995

acc tcc acc ggc ggc gac ggc ctc cta gtt gac ctg tcc acg cct gcc 3139

Thr Ser Thr Gly Gly Asp Gly Leu Leu Val Asp Leu Ser Thr Pro Ala

1000 1005 1010

cgc ctc gac cgc gtc atc ttg acc acc ggc acc ggc tcc gac agc aac 3187

Arg Leu Asp Arg Val Ile Leu Thr Thr Gly Thr Gly Ser Asp Ser Asn

1015 1020 1025

gtg acc tcg acc gtg aag atc tac gca ttc aac gac gcc tca cca cac 3235

Val Thr Ser Thr Val Lys Ile Tyr Ala Phe Asn Asp Ala Ser Pro His

1030 1035 1040 1045

tcc ctg tcg gaa ggc atc gag atc ggc acc gtg gat tat tcc ggc cgc 3283

Ser Leu Ser Glu Gly Ile Glu Ile Gly Thr Val Asp Tyr Ser Gly Arg

1050 1055 1060

agt ctc agc cac agc atc cgc gat tcc tcc aag ctt ccg ggt cag gtg 3331

Ser Leu Ser His Ser Ile Arg Asp Ser Ser Lys Leu Pro Gly Gln Val

1065 1070 1075

gaa tcc gtg gtg att ctg gtc gat gag gtt cgt tcc tca caa acc tca 3379

Glu Ser Val Val Ile Leu Val Asp Glu Val Arg Ser Ser Gln Thr Ser

1080 1085 1090

gac acc aat cca cag atg cag atc gct gaa gta caa ctt gtt ggt tgg 3427

Asp Thr Asn Pro Gln Met Gln Ile Ala Glu Val Gln Leu Val Gly Trp

1095 1100 1105

taaattacgc gtttgtgatt gacccccatt 3457

<210>62

<211>1109

<212>PRT

<213>谷氨酸棒状杆菌

<400>62

Val Ser Ser Ser Leu Ser Asn Asn Ser Glu Gln Ser Gly Leu Arg Gly

1 5 10 15

Arg Ile Val Ala Pro Ala Pro Pro Ala Pro Val Pro Glu Ala Arg Lys

20 25 30

Lys Ala Val Ala Arg Thr Asp Gly Asp Arg Ser Ser Leu Lys Asn Ser

35 40 45

Pro Thr Ala Ser Ala Thr Gln Ala Ala Gln Thr Arg Pro Ala Glu Pro

50 55 60

Glu Pro Lys Lys His Thr Ser Asp Ser Asp Val Val Arg Ser Thr Gly

65 70 75 80

Ser Met Ala Ile Ala Thr Leu Leu Ser Arg Ile Thr Gly Phe Leu Arg

85 90 95

Thr Val Met Ile Gly Ala Ala Leu Ser Pro Ala Ile Ala Ser Ala Phe

100 105 110

Asn Thr Ala Asn Thr Leu Pro Asn Leu Ile Thr Glu Ile Val Leu Gly

115 120 125

Ala Val Leu Thr Ser Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys

130 135 140

Glu Asp Ala Asp Gly Gly Ser Gly Phe Phe Arg Arg Leu Leu Thr Leu

145 150 155 160

Ser Val Thr Leu Leu Gly Gly Val Thr Ile Leu Ser Ile Ile Gly Ala

165 170 175

Pro Leu Leu Thr Arg Met Met Leu Ser Ser Glu Gly Gln Val Asn Val

180 185 190

Val Met Ser Thr Ala Phe Ala Tyr Trp Leu Leu Pro Gln Ile Phe Phe

195 200 205

Tyr Gly Leu Phe Ala Leu Phe Met Ala Val Leu Asn Thr Arg Glu Val

210 215 220

Phe Lys Pro Gly Ala Trp Ala Pro Val Val Asn Asn Val Ile Thr Leu

225 230 235 240

Thr Val Leu Gly Val Tyr Met Val Leu Pro Ala Arg Leu His Pro His

245 250 255

Glu Gln Val Gly Ile Phe Asp Pro Gln Ile Ile Phe Leu Gly Val Gly

260 265 270

Thr Thr Leu Gly Val Val Ala Gln Cys Leu Ile Met Ile Pro Tyr Leu

275 280 285

Arg Arg Ala Gly Ile Asp Met Arg Pro Leu Trp Gly Ile Asp Ala Arg

290 295 300

Leu Lys Gln Phe Gly Gly Met Ala Met Ala Ile Ile Val Tyr Val Ala

305 310 315 320

Ile Ser Gln Phe Gly Tyr Ile Ile Thr Thr Arg Ile Ala Ser Ile Ala

325 330 335

Asp Asp Ala Ala Pro Phe Ile Tyr Gln Gln His Trp Met Leu Leu Gln

340 345 350

Val Pro Tyr Gly Ile Ile Gly Val Thr Leu Leu Thr Ala Ile Met Pro

355 360 365

Arg Leu Ser Arg Asn Ala Ala Asp Gly Asp Asp Arg Ala Val Val Ser

370 375 380

Asp Leu Gln Leu Gly Ser Lys Leu Thr Phe Ile Ala Leu Ile Pro Ile

385 390 395 400

Val Val Phe Phe Thr Ala Phe Gly Val Pro Ile Ala Asn Gly Leu Phe

405 410 415

Ala Tyr Gly Gln Phe Asp Ala Asn Ala Ala Asn Ile Leu Gly Trp Thr

420 425 430

Leu Ser Phe Ser Ala Phe Thr Leu Ile Pro Tyr Ala Leu Val Leu Leu

435 440 445

His Leu Arg Val Phe Tyr Ala Arg Glu Glu Val Trp Thr Pro Thr Phe

450 455 460

Ile Ile Ala Gly Ile Thr Ala Thr Lys Val Val Leu Ser Leu Leu Ala

465 470 475 480

Pro Leu Leu Ser Ser Ser Pro Glu Arg Val Val Val Leu Leu Gly Ala

485 490 495

Ala Asn Gly Phe Ser Phe Ile Thr Gly Ala Val Ile Gly Ala Tyr Leu

500 505 510

Leu Arg Asn Lys Leu Gly Leu Leu Gly Met Arg Ser Leu Ala Lys Thr

515 520 525

Ser Leu Trp Ala Leu Gly Ser Ala Ala Val Gly Ala Ala Ala Ala Trp

530 535 540

Ala Leu Gly Trp Leu Ile Gln Ala Val Val Gly Asp Phe Leu Leu Gly

545 550 555 560

Thr Leu Ser Ser Val Gly Tyr Leu Leu Asn Leu Ala Val Leu Gly Val

565 570 575

Phe Phe Ile Phe Val Thr Gly Ile Val Leu Ser Arg Ser Gly Leu Pro

580 585 590

Glu Val Gln Asn Leu Gly Gln Ala Leu Thr Arg Ile Pro Gly Leu Ser

595 600 605

Arg Phe Ile Arg Pro Asn Thr Lys Ile Ser Leu Asp Val Gly Glu Val

610 615 620

Ser Glu Gln Asp Phe Ser Thr Gln Leu Val Ala Pro Ser Glu Phe Ala

625 630 635 640

Ala Thr Pro Val Pro Pro Pro Met Ser Ala Gly Ile Val Arg Gly Pro

645 650 655

Arg Leu Val Pro Gly Ala Pro Val Gly Asp Gly Arg Phe Arg Leu Leu

660 665 670

Ala Asp His Gly Gly Val Gln Gly Ala Arg Phe Trp Gln Ala Arg Glu

675 680 685

Ile Ala Thr Gly Lys Glu Val Ala Leu Ile Phe Val Asp Thr Ser Gly

690 695 700

Asn Ala Pro Phe Ala Pro Leu Ser Ser Ala Ala Ala Ala Gly Ile Ala

705 710 715 720

Tyr Glu Val Gln Arg Arg Thr Lys Lys Leu Ala Ser Leu Gly Ser Leu

725 730 735

Ala Val Ala Pro Asn Ile His Ser Glu Ala Tyr Arg Asn Gly Cys Leu

740 745 750

Ile Val Ala Asp Trp Val Pro Gly Ser Ser Leu Ser Ala Val Ala Glu

755 760 765

Ser Gly Ala Asp Pro Arg Ala Ala Ala Phe Ala Leu Ala Glu Leu Thr

770 775 780

Glu Thr Ile Gly Glu Ala His Glu Met Gly Ile Pro Ala Gly Leu Asp

785 790 795 800

Asn Lys Cys Arg Ile Arg Ile Asn Thr Asp Gly His Ala Val Leu Ala

805 810 815

Phe Pro Ala Ile Leu Pro Asp Ala Ser Glu Leu Arg Asp Ala Lys Ser

820 825 830

Leu Ala Ser Ala Ala Glu Met Leu Ile Asp Ala Thr Leu Ala Pro Ser

835 840 845

Asp Val Lys Ala Met Val Thr Glu Ala Gln Gly Leu Ala Thr Glu Asp

850 855 860

Asn Pro Asp Tyr Ala Ser Leu Ala Met Ala Met Arg Thr Cys Gly Leu

865 870 875 880

Phe Thr Glu Glu Pro Thr His Leu Val Val Lys Lys Glu Lys Thr Pro

885 890 895

Lys Pro Ala Thr Arg Asp Gly Phe Gly Ala Ser Asp Tyr Thr Val Lys

900 905 910

Gly Met Ala Ala Ile Ala Ala Val Val Ile Ile Leu Val Ser Leu Val

915 920 925

Ala Ala Gly Thr Ala Phe Leu Thr Ser Phe Phe Gly Ser Ser Thr Asn

930 935 940

Glu Gln Ser Pro Leu Ala Ser Val Glu Ala Thr Thr Ser Ala Thr Pro

945 950 955 960

Glu Pro Val Gly Pro Pro Val Tyr Leu Asp Leu Asp Gln Ala Arg Thr

965 970 975

Trp Asp Asp Gly Ala Gly Thr Asp Val Thr Asp Val Thr Asp Gly Asn

980 985 990

Thr Ser Thr Ala Trp Thr Ser Thr Gly Gly Asp Gly Leu Leu Val Asp

995 1000 1005

Leu Ser Thr Pro Ala Arg Leu Asp Arg Val Ile Leu Thr Thr Gly Thr

1010 1015 1020

Gly Ser Asp Ser Asn Val Thr Ser Thr Val Lys Ile Tyr Ala Phe Asn

1025 1030 1035 1040

Asp Ala Ser Pro His Ser Leu Ser Glu Gly Ile Glu Ile Gly Thr Val

1045 1050 1055

Asp Tyr Ser Gly Arg Ser Leu Ser His Ser Ile Arg Asp Ser Ser Lys

1060 1065 1070

Leu Pro Gly Gln Val Glu Ser Val Val Ile Leu Val Asp Glu Val Arg

1075 1080 1085

Ser Ser Gln Thr Ser Asp Thr Asn Pro Gln Met Gln Ile Ala Glu Val

1090 1095 1100

Gln Leu Val Gly Trp

1105

<210>63

<211>1354

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1324)

<223>RXA01442

<400>63

aattaagcaa cctttttaac aggtttagag ctttcacgag gtggcgtcag ataaagtgaa 60

caccaatatt tctgacatct tccaacggag gctaaaaggc atg tac atc cca gag 115

Met Tyr Ile Pro Glu

1 5

tcg atc ggc acc cct ttg acc ccc aat gcc acg aaa gtg atg ctg ctg 163

Ser Ile Gly Thr Pro Leu Thr Pro Asn Ala Thr Lys Val Met Leu Leu

10 15 20

gga tca gga gaa tta ggc aaa gaa gta gcc atc gct ttc cag cgt ctc 211

Gly Ser Gly Glu Leu Gly Lys Glu Val Ala Ile Ala Phe Gln Arg Leu

25 30 35

ggc ctg gaa gtc cat gca gtt gat cgc tac gaa cat gcc cca gcc cac 259

Gly Leu Glu Val His Ala Val Asp Arg Tyr Glu His Ala Pro Ala His

40 45 50

cag gtc gct cac ttc tcc tat gtc atc gac atg aca gat gca gcc cag 307

Gln Val Ala His Phe Ser Tyr Val Ile Asp Met Thr Asp Ala Ala Gln

55 60 65

gtg cgg gaa ttg gtg gag cgt gtg cgc cca gat ttt gtc att cct gaa 355

Val Arg Glu Leu Val Glu Arg Val Arg Pro Asp Phe Val Ile Pro Glu

70 75 80 85

atc gaa gca ctg gca acc gat gaa ctg gtg aag atc gaa gaa gag ggg 403

Ile Glu Ala Leu Ala Thr Asp Glu Leu Val Lys Ile Glu Glu Glu Gly

90 95 100

cta gct acc atc gtg ccc act gca cgt gca gcc aag ctg acc atg aac 451

Leu Ala Thr Ile Val Pro Thr Ala Arg Ala Ala Lys Leu Thr Met Asn

105 110 115

cgc gaa ggc atc cgc aag ctg gcg gca gag gaa ctg ggt ctt cca acc 499

Arg Glu Gly Ile Arg Lys Leu Ala Ala Glu Glu Leu Gly Leu Pro Thr

120 125 130

tcc aac tat gag ttc tgc tcc act ttc gag gaa ttc tcc gca gct gct 547

Ser Asn Tyr Glu Phe Cys Ser Thr Phe Glu Glu Phe Ser Ala Ala Ala

135 140 145

gaa aag ctt ggt tac ccc aac gtg gtg aaa cca gtg atg agt tct tcc 595

Glu Lys Leu Gly Tyr Pro Asn Val Val Lys Pro Val Met Ser Ser Ser

150 155 160 165

ggc aag ggc caa tct gtt ttg cgt agt tca gac gat ctg cag gca gca 643

Gly Lys Gly Gln Ser Val Leu Arg Ser Ser Asp Asp Leu Gln Ala Ala

170 175 180

tgg gat tat gcg atg agc ggt gca cgc gtg gcc aac tcc cgc gtc atc 691

Trp Asp Tyr Ala Met Ser Gly Ala Arg Val Ala Asn Ser Arg Val Ile

185 190 195

gtg gaa gct ttc gtg gaa ttc gat tac gag atc acc ctg ttg aca gta 739

Val Glu Ala Phe Val Glu Phe Asp Tyr Glu Ile Thr Leu Leu Thr Val

200 205 210

agg tcc atc gat ccc acc acc tct aag cct gcg acc tgg ttc tgt gag 787

Arg Ser Ile Asp Pro Thr Thr Ser Lys Pro Ala Thr Trp Phe Cys Glu

215 220 225

ccc att ggg cac cgc caa gaa gac ggc gac tac gtg gaa tcc tgg cag 835

Pro Ile Gly His Arg Gln Glu Asp Gly Asp Tyr Val Glu Ser Trp Gln

230 235 240 245

cca atg gag atg act cct cgc gcg ctg gaa aac gca cgc tca gta gcc 883

Pro Met Glu Met Thr Pro Arg Ala Leu Glu Asn Ala Arg Ser Val Ala

250 255 260

gca cgc atc acc aac gca ttg ggc gga cgc ggc gta ttt ggt gtg gag 931

Ala Arg Ile Thr Asn Ala Leu Gly Gly Arg Gly Val Phe Gly Val Glu

265 270 275

ctc ttt gtc tcc ggc gat gac gtg tac ttc tct gaa gtc tcc cca cgc 979

Leu Phe Val Ser Gly Asp Asp Val Tyr Phe Ser Glu Val Ser Pro Arg

280 285 290

cca cac gac acc ggc ctt gtc acc ctt gcc acc cag cgt ttc tct gaa 1027

Pro His Asp Thr Gly Leu Val Thr Leu Ala Thr Gln Arg Phe Ser Glu

295 300 305

ttt gaa ctc cac gcc aag gca att ctg gga ttg cct gtt gat gtc acc 1075

Phe Glu Leu His Ala Lys Ala Ile Leu Gly Leu Pro Val Asp Val Thr

310 315 320 325

ctg att tct cca ggt gcc tcc gct gtc atc tac ggt ggc atc gaa tct 1123

Leu Ile Ser Pro Gly Ala Ser Ala Val Ile Tyr Gly Gly Ile Glu Ser

330 335 340

gaa ggc gtg agc tac acc ggg ttg gct gaa gcg ctg gca gtg gct gaa 1171

Glu Gly Val Ser Tyr Thr Gly Leu Ala Glu Ala Leu Ala Val Ala Glu

345 350 355

act gat ctt cgt atc ttt gcc aag cca gag gcc ttc acc aag cgt cgc 1219

Thr Asp Leu Arg Ile Phe Ala Lys Pro Glu Ala Phe Thr Lys Arg Arg

360 365 370

atg ggt gtt gca gtg tcc acc gct gag gat gtg gct gca gcc agg gac 1267

Met Gly Val Ala Val Ser Thr Ala Glu Asp Val Ala Ala Ala Arg Asp

375 380 385

cgc gcc act ttg gct gcc gcg gcg atc aag gtt cat cca gga aat tcc 1315

Arg Ala Thr Leu Ala Ala Ala Ala Ile Lys Val His Pro Gly Asn Ser

390 395 400 405

gca gag gct taacatgctg ggaaagcatc ggggcagcat 1354

Ala Glu Ala

<210>64

<211>408

<212>PRT

<213>谷氨酸棒状杆菌

<400>64

Met Tyr Ile Pro Glu Ser Ile Gly Thr Pro Leu Thr Pro Asn Ala Thr

1 5 10 15

Lys Val Met Leu Leu Gly Ser Gly Glu Leu Gly Lys Glu Val Ala Ile

20 25 30

Ala Phe Gln Arg Leu Gly Leu Glu Val His Ala Val Asp Arg Tyr Glu

35 40 45

His Ala Pro Ala His Gln Val Ala His Phe Ser Tyr Val Ile Asp Met

50 55 60

Thr Asp Ala Ala Gln Val Arg Glu Leu Val Glu Arg Val Arg Pro Asp

65 70 75 80

Phe Val Ile Pro Glu Ile Glu Ala Leu Ala Thr Asp Glu Leu Val Lys

85 90 95

Ile Glu Glu Glu Gly Leu Ala Thr Ile Val Pro Thr Ala Arg Ala Ala

100 105 110

Lys Leu Thr Met Asn Arg Glu Gly Ile Arg Lys Leu Ala Ala Glu Glu

115 120 125

Leu Gly Leu Pro Thr Ser Asn Tyr Glu Phe Cys Ser Thr Phe Glu Glu

130 135 140

Phe Ser Ala Ala Ala Glu Lys Leu Gly Tyr Pro Asn Val Val Lys Pro

145 150 155 160

Val Met Ser Ser Ser Gly Lys Gly Gln Ser Val Leu Arg Ser Ser Asp

165 170 175

Asp Leu Gln Ala Ala Trp Asp Tyr Ala Met Ser Gly Ala Arg Val Ala

180 185 190

Asn Ser Arg Val Ile Val Glu Ala Phe Val Glu Phe Asp Tyr Glu Ile

195 200 205

Thr Leu Leu Thr Val Arg Ser Ile Asp Pro Thr Thr Ser Lys Pro Ala

210 215 220

Thr Trp Phe Cys Glu Pro Ile Gly His Arg Gln Glu Asp Gly Asp Tyr

225 230 235 240

Val Glu Ser Trp Gln Pro Met Glu Met Thr Pro Arg Ala Leu Glu Asn

245 250 255

Ala Arg Ser Val Ala Ala Arg Ile Thr Asn Ala Leu Gly Gly Arg Gly

260 265 270

Val Phe Gly Val Glu Leu Phe Val Ser Gly Asp Asp Val Tyr Phe Ser

275 280 285

Glu Val Ser Pro Arg Pro His Asp Thr Gly Leu Val Thr Leu Ala Thr

290 295 300

Gln Arg Phe Ser Glu Phe Glu Leu His Ala Lys Ala Ile Leu Gly Leu

305 310 315 320

Pro Val Asp Val Thr Leu Ile Ser Pro Gly Ala Ser Ala Val Ile Tyr

325 330 335

Gly Gly Ile Glu Ser Glu Gly Val Ser Tyr Thr Gly Leu Ala Glu Ala

340 345 350

Leu Ala Val Ala Glu Thr Asp Leu Arg Ile Phe Ala Lys Pro Glu Ala

355 360 365

Phe Thr Lys Arg Arg Met Gly Val Ala Val Ser Thr Ala Glu Asp Val

370 375 380

Ala Ala Ala Arg Asp Arg Ala Thr Leu Ala Ala Ala Ala Ile Lys Val

385 390 395 400

His Pro Gly Asn Ser Ala Glu Ala

405

<210>65

<211>1402

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1372)

<223>RXA01483

<400>65

ggtggccgat aaggtggaag catctttgcg cgcaatccaa ggtctgctgc gcccttcgcg 60

gcgtgcacgc catgagcaac ttcggaggct gaaaaagtag atg tac ccc tat tcc 115

Met Tyr Pro Tyr Ser

1 5

gac gca gac gct ttt cga cgc cag cct gag cgc gcc aag tcc agc caa 163

Asp Ala Asp Ala Phe Arg Arg Gln Pro Glu Arg Ala Lys Ser Ser Gln

10 15 20

ctg cgt acc agc gcc gta gac acc cgc agc gcg ttc gcc cgc gac cgg 211

Leu Arg Thr Ser Ala Val Asp Thr Arg Ser Ala Phe Ala Arg Asp Arg

25 30 35

gct cgc gtg ctg cat tct gct gct ctt cga cgc ctc gcg gat aaa acc 259

Ala Arg Val Leu His Ser Ala Ala Leu Arg Arg Leu Ala Asp Lys Thr

40 45 50

caa gtg gtt ggc ccc aat gat ggt gat act ccg cgc acc cgg ctg acg 307

Gln Val Val Gly Pro Asn Asp Gly Asp Thr Pro Arg Thr Arg Leu Thr

55 60 65

cac tct ttg gaa gta gct caa att gca cgg gga atc gga gct gga ctg 355

His Ser Leu Glu Val Ala Gln Ile Ala Arg Gly Ile Gly Ala Gly Leu

70 75 80 85

gat ttg gat cct gat ctg tgc gat ctg gca ggg ctg tgc cat gac att 403

Asp Leu Asp Pro Asp Leu Cys Asp Leu Ala Gly Leu Cys His Asp Ile

90 95 100

ggg cat ccg ccg tat gga cac aac ggt gaa aac gcg ttg aat gaa gtt 451

Gly His Pro Pro Tyr Gly His Asn Gly Glu Asn Ala Leu Asn Glu Val

105 110 115

gct gcg gcc tgt gga gga ttt gag ggc aac gcc caa acc ttg cgt att 499

Ala Ala Ala Cys Gly Gly Phe Glu Gly Asn Ala Gln Thr Leu Arg Ile

120 125 130

ctc acg cgt ctg gag cca aaa att gtc tct gat gag ggg gag agc ttt 547

Leu Thr Arg Leu Glu Pro Lys Ile Val Ser Asp Glu Gly Glu Ser Phe

135 140 145

ggg ctg aac ttg tcg cgg gct gct ctt gat gct gca tgt aag tat ccg 595

Gly Leu Asn Leu Ser Arg Ala Ala Leu Asp Ala Ala Cys Lys Tyr Pro

150 155 160 165

tgg gct aaa aca aat gcg gat ggc agt gtc aat aag aaa tac agt gct 643

Trp Ala Lys Thr Asn Ala Asp Gly Ser Val Asn Lys Lys Tyr Ser Ala

170 175 180

tat gac gag gac gca gaa atc ctc gct tgg att aga caa ggc cat gag 691

Tyr Asp Glu Asp Ala Glu Ile Leu Ala Trp Ile Arg Gln Gly His Glu

185 190 195

gat ctt cgc cca ccg atc gaa gcg cag gtc atg gac ttt tct gat gac 739

Asp Leu Arg Pro Pro Ile Glu Ala Gln Val Met Asp Phe Ser Asp Asp

200 205 210

att gcc tac tcg gtg cac gat gtg gaa gac ggt att gta tcc ggg cgt 787

Ile Ala Tyr Ser Val His Asp Val Glu Asp Gly Ile Val Ser Gly Arg

215 220 225

atc gac tta aaa gtg ctg tgg gac ctg gtt gaa tta gca gct ttg gca 835

Ile Asp Leu Lys Val Leu Trp Asp Leu Val Glu Leu Ala Ala Leu Ala

230 235 240 245

gac aaa gga gca gct gct ttc gga ggt tcg cct gca gag ctc atc gag 883

Asp Lys Gly Ala Ala Ala Phe Gly Gly Ser Pro Ala Glu Leu Ile Glu

250 255 260

ggt gca gca tcg ttg cgg gaa ctt ccg gtg gtg gct gcc gct gcg gat 931

Gly Ala Ala Ser Leu Arg Glu Leu Pro Val Val Ala Ala Ala Ala Asp

265 270 275

ttt gat ttc tca ctg cgt tcc tac gct gcg ctg aag gcg atg act tca 979

Phe Asp Phe Ser Leu Arg Ser Tyr Ala Ala Leu Lys Ala Met Thr Ser

280 285 290

gaa cta gtg gga aga tac gtt ggc tct acc atc gag tca aca aag aaa 1027

Glu Leu Val Gly Arg Tyr Val Gly Ser Thr Ile Glu Ser Thr Lys Lys

295 300 305

aca cac gct ggc att gat gtg gga cgc atg cac ggc gat ttg atc att 1075

Thr His Ala Gly Ile Asp Val Gly Arg Met His Gly Asp Leu Ile Ile

310 315 320 325

cca gaa aca gcg gcc agt gaa gta aaa ctg ctc aaa acg tta gcg gtt 1123

Pro Glu Thr Ala Ala Ser Glu Val Lys Leu Leu Lys Thr Leu Ala Val

330 335 340

ctc tac gtg atg gat gac cca ggg cac ctt gcg cgc caa aac agg caa 1171

Leu Tyr Val Met Asp Asp Pro Gly His Leu Ala Arg Gln Asn Arg Gln

345 350 355

cgg gat cgt atc ttc cgg gtt ttt gac tac ctg gtg ctg ggg gct ccg 1219

Arg Asp Arg Ile Phe Arg Val Phe Asp Tyr Leu Val Leu Gly Ala Pro

360 365 370

gga tcg ttg gat ccg atg tat cgc cag tgg ttt att gaa gcg gat tca 1267

Gly Ser Leu Asp Pro Met Tyr Arg Gln Trp Phe Ile Glu Ala Asp Ser

375 380 385

gaa tcg gaa cag atc cgt gtg att gtt gat cag att gcg tcg atg acg 1315

Glu Ser Glu Gln Ile Arg Val Ile Val Asp Gln Ile Ala Ser Met Thr

390 395 400 405

gag tct cgt ctg gaa cgc ctt gcc cgg aat gct gct gac atc tca gga 1363

Glu Ser Arg Leu Glu Arg Leu Ala Arg Asn Ala Ala Asp Ile Ser Gly

410 415 420

ttt ttg gga taattggtta gagcagcagt aagtttcagt 1402

Phe Leu Gly

<210>66

<211>424

<212>PRT

<213>谷氨酸棒状杆菌

<400>66

Met Tyr Pro Tyr Ser Asp Ala Asp Ala Phe Arg Arg Gln Pro Glu Arg

1 5 10 15

Ala Lys Ser Ser Gln Leu Arg Thr Ser Ala Val Asp Thr Arg Ser Ala

20 25 30

Phe Ala Arg Asp Arg Ala Arg Val Leu His Ser Ala Ala Leu Arg Arg

35 40 45

Leu Ala Asp Lys Thr Gln Val Val Gly Pro Asn Asp Gly Asp Thr Pro

50 55 60

Arg Thr Arg Leu Thr His Ser Leu Glu Val Ala Gln Ile Ala Arg Gly

65 70 75 80

Ile Gly Ala Gly Leu Asp Leu Asp Pro Asp Leu Cys Asp Leu Ala Gly

85 90 95

Leu Cys His Asp Ile Gly His Pro Pro Tyr Gly His Asn Gly Glu Asn

100 105 110

Ala Leu Asn Glu Val Ala Ala Ala Cys Gly Gly Phe Glu Gly Asn Ala

115 120 125

Gln Thr Leu Arg Ile Leu Thr Arg Leu Glu Pro Lys Ile Val Ser Asp

130 135 140

Glu Gly Glu Ser Phe Gly Leu Asn Leu Ser Arg Ala Ala Leu Asp Ala

145 150 155 160

Ala Cys Lys Tyr Pro Trp Ala Lys Thr Asn Ala Asp Gly Ser Val Asn

165 170 175

Lys Lys Tyr Ser Ala Tyr Asp Glu Asp Ala Glu Ile Leu Ala Trp Ile

180 185 190

Arg Gln Gly His Glu Asp Leu Arg Pro Pro Ile Glu Ala Gln Val Met

195 200 205

Asp Phe Ser Asp Asp Ile Ala Tyr Ser Val His Asp Val Glu Asp Gly

210 215 220

Ile Val Ser Gly Arg Ile Asp Leu Lys Val Leu Trp Asp Leu Val Glu

225 230 235 240

Leu Ala Ala Leu Ala Asp Lys Gly Ala Ala Ala Phe Gly Gly Ser Pro

245 250 255

Ala Glu Leu Ile Glu Gly Ala Ala Ser Leu Arg Glu Leu Pro Val Val

260 265 270

Ala Ala Ala Ala Asp Phe Asp Phe Ser Leu Arg Ser Tyr Ala Ala Leu

275 280 285

Lys Ala Met Thr Ser Glu Leu Val Gly Arg Tyr Val Gly Set Thr Ile

290 295 300

Glu Ser Thr Lys Lys Thr His Ala Gly Ile Asp Val Gly Arg Met His

305 310 315 320

Gly Asp Leu Ile Ile Pro Glu Thr Ala Ala Ser Glu Val Lys Leu Leu

325 330 335

Lys Thr Leu Ala Val Leu Tyr Val Met Asp Asp Pro Gly His Leu Ala

340 345 350

Arg Gln Asn Arg Gln Arg Asp Arg Ile Phe Arg Val Phe Asp Tyr Leu

355 360 365

Val Leu Gly Ala Pro Gly Ser Leu Asp Pro Met Tyr Arg Gln Trp Phe

370 375 380

Ile Glu Ala Asp Ser Glu Ser Glu Gln Ile Arg Val Ile Val Asp Gln

385 390 395 400

Ile Ala Ser Met Thr Glu Ser Arg Leu Glu Arg Leu Ala Arg Asn Ala

405 410 415

Ala Asp Ile Ser Gly Phe Leu Gly

420

<210>67

<211>982

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(952)

<223>RXA01515

<400>67

taagcctggt gctgtgacca cgacgtctgc ggtgcgcggc ggttttaaga acaacgctgc 60

ctcccgcgct gaggtgttct ccctgattcg ggggcactaa atg aac gta tcc tct 115

Met Asn Val Ser Ser

1 5

ttg acc atc ccg gga cgc tgt ttg gtc atg gga att gtc aat gtc act 163

Leu Thr Ile Pro Gly Arg Cys Leu Val Met Gly Ile Val Asn Val Thr

10 15 20

gag gat tcc ttt tcg gac ggt ggc aag tac att gac gtt gat cag gcg 211

Glu Asp Ser Phe Ser Asp Gly Gly Lys Tyr Ile Asp Val Asp Gln Ala

25 30 35

atc gcg cat gcc aag gaa ttg gtg gct gct ggc gcc gac atg att gat 259

Ile Ala His Ala Lys Glu Leu Val Ala Ala Gly Ala Asp Met Ile Asp

40 45 50

gtc ggc ggc gag tcc acc cgg cct ggg gca gtg cgc gtc gac gcg tcc 307

Val Gly Gly Glu Ser Thr Arg Pro Gly Ala Val Arg Val Asp Ala Ser

55 60 65

gtg gaa cgg gac cgg gtt gtg ccg gtc att aag gcg ctt cac gac gcc 355

Val Glu Arg Asp Arg Val Val Pro Val Ile Lys Ala Leu His Asp Ala

70 75 80 85

ggc atc cac act tcc gta gac acc atg cgg gcc tcc gtg gcg cag gct 403

Gly Ile His Thr Ser Val Asp Thr Met Arg Ala Ser Val Ala Gln Ala

90 95 100

gcc gcg ggc gct ggc gtc tcc atg atc aac gac gtc tct ggc ggt ttg 451

Ala Ala Gly Ala Gly Val Ser Met Ile Asn Asp Val Ser Gly Gly Leu

105 110 115

gct gat cct gag atg ttt tct gtc atg gcg gaa gcg caa att ccc gtg 499

Ala Asp Pro Glu Met Phe Ser Val Met Ala Glu Ala Gln Ile Pro Val

120 125 130

tgt ttg atg cac tgg cgc acc ctc caa ttc ggt gat gcc gca ggt cag 547

Cys Leu Met His Trp Arg Thr Leu Gln Phe Gly Asp Ala Ala Gly Gln

135 140 145

gca gat cac ggt gga gac gtt gta gcc gat gtg cac gca gtg ctt gat 595

Ala Asp His Gly Gly Asp Val Val Ala Asp Val His Ala Val Leu Asp

150 155 160 165

gat ctt gtc gcc cgc gcc acc gct gct ggt gtg gcc gaa aac cag atc 643

Asp Leu Val Ala Arg Ala Thr Ala Ala Gly Val Ala Glu Asn Gln Ile

170 175 180

gtg ctt gat cca ggt ttg ggt ttt gcc aaa tca cgt gaa gac aac tgg 691

Val Leu Asp Pro Gly Leu Gly Phe Ala Lys SeT Arg Glu Asp Asn Trp

185 190 195

cgt ttg ctg caa gca ctg ccc gag ttt att tct gga cct ttc ccc atc 739

Arg Leu Leu Gln Ala Leu Pro Glu Phe Ile Ser Gly Pro Phe Pro Ile

200 205 210

ctg gtg gga gca tcc cgg aag cga ttc ctg gct ggc gtg cgc aaa gac 787

Leu Val Gly Ala Ser Arg Lys Arg Phe Leu Ala Gly Val Arg Lys Asp

215 220 225

cgt ggc cta gat gtc acc ccc att gat gcc gac cca gca acc gca gcg 835

Arg Gly Leu Asp Val Thr Pro Ile Asp Ala Asp Pro Ala Thr Ala Ala

230 235 240 245

gtg acc gca gtg tct gca cat atg gga gca tgg ggt gtg cgc gtg cac 883

Val Thr Ala Val Ser Ala His Met Gly Ala Trp Gly Val Arg Val His

250 255 260

gat gtc cca gta tca agg gac gct gtt gat gtt gcc gca ttg tgg cga 931

Asp Val Pro Val Ser Arg Asp Ala Val Asp Val Ala Ala Leu Trp Arg

265 270 275

agt gga gga act cac cat ggc tgatcgtatt gaacttaaag gccttgaatg 982

Ser Gly Gly Thr His His Gly

280

<210>68

<211>284

<212>PRT

<213>谷氨酸棒状杆菌

<400>68

Met Asn Val Ser Ser Leu Thr Ile Pro Gly Arg Cys Leu Val Met Gly

1 5 10 15

Ile Val Asn Val Thr Glu Asp Ser Phe Ser Asp Gly Gly Lys Tyr Ile

20 25 30

Asp Val Asp Gln Ala Ile Ala His Ala Lys Glu Leu Val Ala Ala Gly

35 40 45

Ala Asp Met Ile Asp Val Gly Gly Glu Ser Thr Arg Pro Gly Ala Val

50 55 60

Arg Val Asp Ala Ser Val Glu Arg Asp Arg Val Val Pro Val Ile Lys

65 70 75 80

Ala Leu His Asp Ala Gly Ile His Thr Ser Val Asp Thr Met Arg Ala

85 90 95

Ser Val Ala Gln Ala Ala Ala Gly Ala Gly Val Ser Met Ile Asn Asp

100 105 110

Val Ser Gly Gly Leu Ala Asp Pro Glu Met Phe Ser Val Met Ala Glu

115 120 125

Ala Gln Ile Pro Val Cys Leu Met His Trp Arg Thr Leu Gln Phe Gly

130 135 140

Asp Ala Ala Gly Gln Ala Asp His Gly Gly Asp Val Val Ala Asp Val

145 150 155 160

His Ala Val Leu Asp Asp Leu Val Ala Arg Ala Thr Ala Ala Gly Val

165 170 175

Ala Glu Asn Gln Ile Val Leu Asp Pro Gly Leu Gly Phe Ala Lys Ser

180 185 190

Arg Glu Asp Asn Trp Arg Leu Leu Gln Ala Leu Pro Glu Phe Ile Ser

195 200 205

Gly Pro Phe Pro Ile Leu Val Gly Ala Ser Arg Lys Arg Phe Leu Ala

210 215 220

Gly Val Arg Lys Asp Arg Gly Leu Asp Val Thr Pro Ile Asp Ala Asp

225 230 235 240

Pro Ala Thr Ala Ala Val Thr Ala Val Ser Ala His Met Gly Ala Trp

245 250 255

Gly Val Arg Val His Asp Val Pro Val Ser Arg Asp Ala Val Asp Val

260 265 270

Ala Ala Leu Trp Arg Ser Gly Gly Thr His His Gly

275 280

<210>69

<211>604

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(574)

<223>RXA01518

<400>69

agcagaacct gatgccgtcc tgcacggcac gaccattgca gaacatgtgg ataatcttga 60

tcccacagac attgaaggtg tcaccaagat ttaaggagtc gtg gct ttc atg cag 115

Val Ala Phe Met Gln

1 5

aaa acg tca gcg ggt tgg ttg atc gca acg gga ggt ttc ctc gcc gct 163

Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly Gly Phe Leu Ala Ala

10 15 20

gtg tcc gcc att ttg acg tgg cgt ttt tat gga tcc atg acc tct att 211

Val Ser Ala Ile Leu Thr Trp Arg Phe Tyr Gly Ser Met Thr Ser Ile

25 30 35

tcc atc acg gta tcc atc act ttt tgg ttg ttg gcc gtg gtg tgt ggt 259

Ser Ile Thr Val Ser Ile Thr Phe Trp Leu Leu Ala Val Val Cys Gly

40 45 50

ttc gca ggt gtg aag gtc caa ggt cgc ctc gat gag ggg ctg atc ggc 307

Phe Ala Gly Val Lys Val Gln Gly Arg Leu Asp Glu Gly Leu Ile Gly

55 60 65

cag gac aaa tcc caa atg aac ccc gtg acc att gcc tat ctg gcc atg 355

Gln Asp Lys Ser Gln Met Asn Pro Val Thr Ile Ala Tyr Leu Ala Met

70 75 80 85

ctg ggt cga gcg tgt gcg tgg ggt ggc gca att ttc ggc ggc gtt tat 403

Leu Gly Arg Ala Cys Ala Trp Gly Gly Ala Ile Phe Gly Gly Val Tyr

90 95 100

gtg gga att ggc agt tat gta atc cca cgc gcc ggt gag ttg tcc gca 451

Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala Gly Glu Leu Ser Ala

105 110 115

gca tcg aat gat ctt ccg gga gtt att gcc tgt gcg ctg ggc gga atc 499

Ala Ser Asn Asp Leu Pro Gly Val Ile Ala Cys Ala Leu Gly Gly Ile

120 125 130

gca ctc tca gct gcc gga ctt tat tta gag cga agc tgt gag gct ccg 547

Ala Leu Ser Ala Ala Gly Leu Tyr Leu Glu Arg Ser Cys Glu Ala Pro

135 140 145

cct ccc caa tct ggc gaa gcg atc agc tagattggaa ttcatgaatc 594

Pro Pro Gln Ser Gly Glu Ala Ile Ser

150 155

aagacctgtc 604

<210>70

<211>158

<212>PRT

<213>谷氨酸棒状杆菌

<400>70

Val Ala Phe Met Gln Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly

1 5 10 15

Gly Phe Leu Ala Ala Val Ser Ala Ile Leu Thr Trp Arg Phe Tyr Gly

20 25 30

Ser Met Thr Ser Ile Ser Ile Thr Val Ser Ile Thr Phe Trp Leu Leu

35 40 45

Ala Val Val Cys Gly Phe Ala Gly Val Lys Val Gln Gly Arg Leu Asp

50 55 60

Glu Gly Leu Ile Gly Gln Asp Lys Ser Gln Met Asn Pro Val Thr Ile

65 70 75 80

Ala Tyr Leu Ala Met Leu Gly Arg Ala Cys Ala Trp Gly Gly Ala Ile

85 90 95

Phe Gly Gly Val Tyr Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala

100 105 110

Gly Glu Leu Ser Ala Ala Ser Asn Asp Leu Pro Gly Val Ile Ala Cys

115 120 125

Ala Leu Gly Gly Ile Ala Leu Ser Ala Ala Gly Leu Tyr Leu Glu Arg

130 135 140

Ser Cys Glu Ala Pro Pro Pro Gln Ser Gly Glu Ala Ile Ser

145 150 155

<210>71

<211>1267

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1237)

<223>RXA01690

<400>71

gggggaggca tcaaattgaa actaactttt aacaagccta gccattcctc aaaaccgtga 60

gacgaaattg gctattcatc ccataaaatg gggctgacta gtg tat ctg tca ggt 115

Val Tyr Leu Ser Gly

1 5

agc agg tgt acc tta aaa tcc atg acg tca tta gag ttc aca gta acc 163

Ser Arg Cys Thr Leu Lys Ser Met Thr Ser Leu Glu Phe Thr Val Thr

10 15 20

cgt acc gaa aat ccg acg tca ccc gat cgt ctg aag gaa att ctt gcc 211

Arg Thr Glu Asn Pro Thr Ser Pro Asp Arg Leu Lys Glu Ile Leu Ala

25 30 35

gca ccg aag ttc ggt aag ttc ttc acc gac cac atg gtg acc att gac 259

Ala Pro Lys Phe Gly Lys Phe Phe Thr Asp His Met Val Thr Ile Asp

40 45 50

tgg aac gag tcg gaa ggc tgg cac aac gcc caa tta gtg cca tac gcg 307

Trp Asn Glu Ser Glu Gly Trp His Asn Ala Gln Leu Val Pro Tyr Ala

55 60 65

ccg att cct atg gat cct gcc acc acc gta ttc cac tac gga cag gca 355

Pro Ile Pro Met Asp Pro Ala Thr Thr Val Phe His Tyr Gly Gln Ala

70 75 80 85

att ttt gag gga att aag gcc tac cgc cat tcg gac gaa acc atc aag 403

Ile Phe Glu Gly Ile Lys Ala Tyr Arg His Ser Asp Glu Thr Ile Lys

90 95 100

act ttc cgt cct gat gaa aac gcc gag cgt atg cag cgt tca gca gct 451

Thr Phe Arg Pro Asp Glu Asn Ala Glu Arg Met Gln Arg Ser Ala Ala

105 110 115

cga atg gca atg cca cag ttg cca acc gag gac ttt att aaa gca ctt 499

Arg Met Ala Met Pro Gln Leu Pro Thr Glu Asp Phe Ile Lys Ala Leu

120 125 130

gaa ctg ctg gta gac gcg gat cag gat tgg gtt cct gag tac ggc gga 547

Glu Leu Leu Val Asp Ala Asp Gln Asp Trp Val Pro Glu Tyr Gly Gly

135 140 145

gaa gct tcc ctc tac ctg cgc cca ttc atg atc tcc acc gaa att ggc 595

Glu Ala Ser Leu Tyr Leu Arg Pro Phe Met Ile Ser Thr Glu Ile Gly

150 155 160 165

ttg ggt gtc agc cca gct gat gcc tac aag ttc ctg gtc atc gca tcc 643

Leu Gly Val Ser Pro Ala Asp Ala Tyr Lys Phe Leu Val Ile Ala Ser

170 175 180

cca gtc ggc gct tac ttc acc ggt gga atc aag cct gtt tcc gtc tgg 691

Pro Val Gly Ala Tyr Phe Thr Gly Gly Ile Lys Pro Val Ser Val Trp

185 190 195

ctg agc gag gat tac gtc cgc gct gca ccc ggc gga act ggt gac gcc 739

Leu Ser Glu Asp Tyr Val Arg Ala Ala Pro Gly Gly Thr Gly Asp Ala

200 205 210

aaa ttt gct ggc aac tac gcg gct tct ttg ctt gcc cag tcc cag gct 787

Lys Phe Ala Gly Asn Tyr Ala Ala Ser Leu Leu Ala Gln Ser Gln Ala

215 220 225

gcg gaa aag ggc tgt gac cag gtc gta tgg ttg gat gcc atc gag cac 835

Ala Glu Lys Gly Cys Asp Gln Val Val Trp Leu Asp Ala Ile Glu His

230 235 240 245

aag tac atc gaa gaa atg ggt ggc atg aac ctt ggg ttc atc tac cgc 883

Lys Tyr Ile Glu Glu Met Gly Gly Met Asn Leu Gly Phe Ile Tyr Arg

250 255 260

aac ggc gac caa gtc aag cta gtc acc cct gaa ctt tcc ggc tca cta 931

Asn Gly Asp Gln Val Lys Leu Val Thr Pro Glu Leu Ser Gly Ser Leu

265 270 275

ctt cca ggc atc acc cgc aag tca ctt cta caa gta gca cgc gac ttg 979

Leu Pro Gly Ile Thr Arg Lys Ser Leu Leu Gln Val Ala Arg Asp Leu

280 285 290

gga tac gaa gta gaa gag cga aag atc acc acc acc gag tgg gaa gaa 1027

Gly Tyr Glu Val Glu Glu Arg Lys Ile Thr Thr Thr Glu Trp Glu Glu

295 300 305

gac gca aag tct ggc gcc atg acc gag gca ttt gct tgc ggt act gca 1075

Asp Ala Lys Ser Gly Ala Met Thr Glu Ala Phe Ala Cys Gly Thr Ala

310 315 320 325

gct gtt atc acc cct gtt ggc acc gtg aaa tca gct cac ggc acc ttc 1123

Ala Val Ile Thr Pro Val Gly Thr Val Lys Ser Ala His Gly Thr Phe

330 335 340

gaa gtg aac aac aat gaa gtc gga gaa atc acg atg aag ctt cgt gaa 1171

Glu Val Asn Asn Asn Glu Val Gly Glu Ile Thr Met Lys Leu Arg Glu

345 350 355

acc ctc acc gga att cag caa gga aac gtt gaa gac caa aac gga tgg 1219

Thr Leu Thr Gly Ile Gln Gln Gly Asn Val Glu Asp Gln Asn Gly Trp

360 365 370

ctt tac cca ctg gtt ggc taaatcaacc ggttttaaga ccccgctgca 1267

Leu Tyr Pro Leu Val Gly

375

<210>72

<211>379

<212>PRT

<213>谷氨酸棒状杆菌

<400>72

Val Tyr Leu Ser Gly Ser Arg Cys Thr Leu Lys Ser Met Thr Ser Leu

1 5 10 15

Glu Phe Thr Val Thr Arg Thr Glu Asn Pro Thr Ser Pro Asp Arg Leu

20 25 30

Lys Glu Ile Leu Ala Ala Pro Lys Phe Gly Lys Phe Phe Thr Asp His

35 40 45

Met Val Thr Ile Asp Trp Asn Glu Ser Glu Gly Trp His Asn Ala Gln

50 55 60

Leu Val Pro Tyr Ala Pro Ile Pro Met Asp Pro Ala Thr Thr Val Phe

65 70 75 80

His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala Tyr Arg His Ser

85 90 95

Asp Glu Thr Ile Lys Thr Phe Arg Pro Asp Glu Asn Ala Glu Arg Met

100 105 110

Gln Arg Ser Ala Ala Arg Met Ala Met Pro Gln Leu Pro Thr Glu Asp

115 120 125

Phe Ile Lys Ala Leu Glu Leu Leu Val Asp Ala Asp Gln Asp Trp Val

130 135 140

Pro Glu Tyr Gly Gly Glu Ala Ser Leu Tyr Leu Arg Pro Phe Met Ile

145 150 155 160

Ser Thr Glu Ile Gly Leu Gly Val Ser Pro Ala Asp Ala Tyr Lys Phe

165 170 175

Leu Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr Gly Gly Ile Lys

180 185 190

Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg Ala Ala Pro Gly

195 200 205

Gly Thr Gly Asp Ala Lys Phe Ala Gly Asn Tyr Ala Ala Ser Leu Leu

210 215 220

Ala Gln Ser Gln Ala Ala Glu Lys Gly Cys Asp Gln Val Val Trp Leu

225 230 235 240

Asp Ala Ile Glu His Lys Tyr Ile Glu Glu Met Gly Gly Met Asn Leu

245 250 255

Gly Phe Ile Tyr Arg Asn Gly Asp Gln Val Lys Leu Val Thr Pro Glu

260 265 270

Leu Ser Gly Ser Leu Leu Pro Gly Ile Thr Arg Lys Ser Leu Leu Gln

275 280 285

Val Ala Arg Asp Leu Gly Tyr Glu Val Glu Glu Arg Lys Ile Thr Thr

290 295 300

Thr Glu Trp Glu Glu Asp Ala Lys Ser Gly Ala Met Thr Glu Ala Phe

305 310 315 320

Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly Thr Val Lys Ser

325 330 335

Ala His Gly Thr Phe Glu Val Asn Asn Asn Glu Val Gly Glu Ile Thr

340 345 350

Met Lys Leu Arg Glu Thr Leu Thr Gly Ile Gln Gln Gly Asn Val Glu

355 360 365

Asp Gln Asn Gly Trp Leu Tyr Pro Leu Val Gly

370 375

<210>73

<211>910

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(880)

<223>RXA01746

<400>73

ctgcagctct aagatctctg caagttaaaa ccgccactcc cctttcactg gggagtggcg 60

gttttgtcgt ttcatgcatg cagtgtgtga cttatcaacc ttg tta ggg cta ggg 115

Leu Leu Gly Leu Gly

1 5

tgg ata tct atc atg act gca cca aga gat cct ttt ttc ccc gca gat 163

Trp Ile Ser Ile Met Thr Ala Pro Arg Asp Pro Phe Phe Pro Ala Asp

10 15 20

ctt tct atc cgc gcg tct gca gag ccc att gaa att cag cgg ttg ggt 211

Leu Ser Ile Arg Ala Ser Ala Glu Pro Ile Glu Ile Gln Arg Leu Gly

25 30 35

ttg atc gat tat caa gag gcc tgg gat tat caa gca gag ctt gct acc 259

Leu Ile Asp Tyr Gln Glu Ala Trp Asp Tyr Gln Ala Glu Leu Ala Thr

40 45 50

cgt agg gct aat gat gaa atc cct gat cag ctg ctt att ttg gag cac 307

Arg Arg Ala Asn Asp Glu Ile Pro Asp Gln Leu Leu Ile Leu Glu His

55 60 65

ccg tcg gtg tat acc gca ggt aag cgc acc cag ccg gaa gat ctt ccc 355

Pro Ser Val Tyr Thr Ala Gly Lys Arg Thr Gln Pro Glu Asp Leu Pro

70 75 80 85

acc aac gga ctg ccg gtg atc aat gct gat cgt ggt ggt cgc atc acg 403

Thr Asn Gly Leu Pro Val Ile Asn Ala Asp Arg Gly Gly Arg Ile Thr

90 95 100

tgg cat ggt cct ggc caa ttg gtg atc tat ccg atc atc aaa tta gcc 451

Trp His Gly Pro Gly Gln Leu Val Ile Tyr Pro Ile Ile Lys Leu Ala

105 110 115

gat ccg atc gat gtg gtt gat tac gta aga cgc ctc gag gaa gcg ctc 499

Asp Pro Ile Asp Val Val Asp Tyr Val Arg Arg Leu Glu Glu Ala Leu

120 125 130

atc caa gtt gtc ggc gat atg ggt gtt gcc ggc gct ggg cgc att gat 547

Ile Gln Val Val Gly Asp Met Gly Val Ala Gly Ala Gly Arg Ile Asp

135 140 145

ggg cgt tcg ggt gtg tgg gtg cca gct cat gat ggt tgg gtg gac agc 595

Gly Arg Ser Gly Val Trp Val Pro Ala His Asp Gly Trp Val Asp Ser

150 155 160 165

aag gtt gcg gcc atc ggc att cga ata act cgt ggt gtt gca atg cac 643

Lys Val Ala Ala Ile Gly Ile Arg Ile Thr Arg Gly Val Ala Met His

170 175 180

ggt gtg gcc atc aac tgc aac aac acg ttg gat ttc tat gag cac atc 691

Gly Val Ala Ile Asn Cys Asn Asn Thr Leu Asp Phe Tyr Glu His Ile

185 190 195

att ccg tgt ggc att gct gat gca ggc ttg agc aca ctc tcg agg gaa 739

Ile Pro Cys Gly Ile Ala Asp Ala Gly Leu Ser Thr Leu Ser Arg Glu

200 205 210

ctg aaa agg gac gtt tca gtt gag gaa tta gtc gag cca tcg atc cgc 787

Leu Lys Arg Asp Val Ser Val Glu Glu Leu Val Glu Pro Ser Ile Arg

215 220 225

gca ttg gat gat gct ttg gct ggt cgg ctg gtt gtt tct gat cat tct 835

Ala Leu Asp Asp Ala Leu Ala Gly Arg Leu Val Val Ser Asp His Ser

230 235 240 245

ttc ggc agc gcg ccc gac cca act aag aat ctc cct aaa cgg ggg 880

Phe Gly Ser Ala Pro Asp Pro Thr Lys Asn Leu Pro Lys Arg Gly

250 255 260

tagtacgagg aattttgtcg gtggggcgcc 910

<210>74

<211>260

<212>PRT

<213>谷氨酸棒状杆菌

<400>74

Leu Leu Gly Leu Gly Trp Ile Ser Ile Met Thr Ala Pro Arg Asp Pro

1 5 10 15

Phe Phe Pro Ala Asp Leu Ser Ile Arg Ala Ser Ala Glu Pro Ile Glu

20 25 30

Ile Gln Arg Leu Gly Leu Ile Asp Tyr Gln Glu Ala Trp Asp Tyr Gln

35 40 45

Ala Glu Leu Ala Thr Arg Arg Ala Asn Asp Glu Ile Pro Asp Gln Leu

50 55 60

Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg Thr Gln

65 70 75 80

Pro Glu Asp Leu Pro Thr Asn Gly Leu Pro Val Ile Asn Ala Asp Arg

85 90 95

Gly Gly Arg Ile Thr Trp His Gly Pro Gly Gln Leu Val Ile Tyr Pro

100 105 110

Ile Ile Lys Leu Ala Asp Pro Ile Asp Val Val Asp Tyr Val Arg Arg

115 120 125

Leu Glu Glu Ala Leu Ile Gln Val Val Gly Asp Met Gly Val Ala Gly

130 135 140

Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala His Asp

145 150 155 160

Gly Trp Val Asp Ser Lys Val Ala Ala Ile Gly Ile Arg Ile Thr Arg

165 170 175

Gly Val Ala Met His Gly Val Ala Ile Asn Cys Asn Asn Thr Leu Asp

180 185 190

Phe Tyr Glu His Ile Ile Pro Cys Gly Ile Ala Asp Ala Gly Leu Ser

195 200 205

Thr Leu Ser Arg Glu Leu Lys Arg Asp Val Ser Val Glu Glu Leu Val

210 215 220

Glu Pro Ser Ile Arg Ala Leu Asp Asp Ala Leu Ala Gly Arg Leu Val

225 230 235 240

Val Ser Asp His Ser Phe Gly Ser Ala Pro Asp Pro Thr Lys Asn Leu

245 250 255

Pro Lys Arg Gly

260

<210>75

<211>1207

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1177)

<223>RXA01810

<400>75

ccaccaccac aatgttgctc atcctcgcgg cgttcgttgt cgcaggtggc tccatgactc 60

gcttcaccgt cggcaacccg actggaaaat aaggcttcac atg aat aac gct ttt 115

Met Asn Asn Ala Phe

1 5

cga cgc acc ctt aca tcc gta gtc ctc gcc gct agc ttg gcc tta acg 163

Arg Arg Thr Leu Thr Ser Val Val Leu Ala Ala Ser Leu Ala Leu Thr

10 15 20

gcc tgc gca agc tgg gat tca cct acg gca tct tcc aat ggt gat ctg 211

Ala Cys Ala Ser Trp Asp Ser Pro Thr Ala Ser Ser Asn Gly Asp Leu

25 30 35

att gag gag atc cag gca agc tcc acc tca aca gat ccg cgc acc ttc 259

Ile Glu Glu Ile Gln Ala Ser Ser Thr Ser Thr Asp Pro Arg Thr Phe

40 45 50

aca ggc ttg agc atc gtg gaa gat atc ggc gat gtg gtt ccc gta acc 307

Thr Gly Leu Ser Ile Val Glu Asp Ile Gly Asp Val Val Pro Val Thr

55 60 65

gac aac gcc tca cca gct ctg cca gtt tct ttg acc gac gct gat ggc 355

Asp Asn Ala Ser Pro Ala Leu Pro Val Ser Leu Thr Asp Ala Asp Gly

70 75 80 85

aac gac gtg gtg gtg gag gac gtg tcc cgc atc ctc cca ctg gat ctc 403

Asn Asp Val Val Val Glu Asp Val Ser Arg Ile Leu Pro Leu Asp Leu

90 95 100

tac gga acc tat tcc aaa acc atc gct ggc ctg gga ctc gtg gac aat 451

Tyr Gly Thr Tyr Ser Lys Thr Ile Ala Gly Leu Gly Leu Val Asp Asn

105 110 115

att gtg ggt cgt act gtt agt tcc acc gag cct gca ttg gcg gac act 499

Ile Val Gly Arg Thr Val Ser Ser Thr Glu Pro Ala Leu Ala Asp Thr

120 125 130

gag gtg gtc acc act ggc gga cac acc ctc aat gct gaa gcg atc ctt 547

Glu Val Val Thr Thr Gly Gly His Thr Leu Asn Ala Glu Ala Ile Leu

135 140 145

aat tta cat ccg act ttg gtg atc atc gac cac tcg atc ggc cca cgc 595

Asn Leu His Pro Thr Leu Val Ile Ile Asp His Ser Ile Gly Pro Arg

150 155 160 165

gaa gtc atc gat cag atc cgc gca gct ggt gtc gcc acg gtg atc atg 643

Glu Val Ile Asp Gln Ile Arg Ala Ala Gly Val Ala Thr Val Ile Met

170 175 180

tcg ccg cag cgt tcc att gcc tca att ggc gac gac atc cgc gac atc 691

Ser Pro Gln Arg Ser Ile Ala Ser Ile Gly Asp Asp Ile Arg Asp Ile

185 190 195

gcc tcc gtc gtt gga ctt cct gaa gaa ggg gag aag ctc gcg gaa cgt 739

Ala Ser Val Val Gly Leu Pro Glu Glu Gly Glu Lys Leu Ala Glu Arg

200 205 210

tcc gtt gct gaa gtc gaa gag gcc agc acg gtt gtc gat gaa ctc acc 787

Ser Val Ala Glu Val Glu Glu Ala Ser Thr Val Val Asp Glu Leu Thr

215 220 225

cca gaa gat ccc ctc aaa atg gta ttc ctc tat gcc cgc gga act ggt 835

Pro Glu Asp Pro Leu Lys Met Val Phe Leu Tyr Ala Arg Gly Thr Gly

230 235 240 245

gga gtg ttc ttc att ttg ggc gat gcc tat ggt gga cgc gat ctc att 883

Gly Val Phe Phe Ile Leu Gly Asp Ala Tyr Gly Gly Arg Asp Leu Ile

250 255 260

gaa ggc ctg ggc ggc gtc gac atg gct gct gaa aag ggc atc atg gat 931

Glu Gly Leu Gly Gly Val Asp Met Ala Ala Glu Lys Gly Ile Met Asp

265 270 275

ctg gca cca gcc aac gcg gaa gca ctt gcc gaa cta aat cca gac gtc 979

Leu Ala Pro Ala Asn Ala Glu Ala Leu Ala Glu Leu Asn Pro Asp Val

280 285 290

ttc gtg atg atg tcg gaa gga cta gtc tcg aca gga ggt atc gac ggt 1027

Phe Val Met Met Ser Glu Gly Leu Val Ser Thr Gly Gly Ile Asp Gly

295 300 305

ctt atg gaa cgc ccc ggc att gct cag aca acc gcc gga caa aac caa 1075

Leu Met Glu Arg Pro Gly Ile Ala Gln Thr Thr Ala Gly Gln Asn Gln

310 315 320 325

cga gta ctg gcg ctt ccc gat ggt caa tca ttg gcc ttt ggt gcc caa 1123

Arg Val Leu Ala Leu Pro Asp Gly Gln Ser Leu Ala Phe Gly Ala Gln

330 335 340

act ggc gag ttg ttg ctc cgc gca tcc cgc gaa ctg tat gtg cag ggc 1171

Thr Gly Glu Leu Leu Leu Arg Ala Ser Arg Glu Leu Tyr Val Gln Gly

345 350 355

ggc gag tagatggttg tgaaggaggt tgacgtcgaa 1207

Gly Glu

<210>76

<211>359

<212>PRT

<213>谷氨酸棒状杆菌

<400>76

Met Asn Asn Ala Phe Arg Arg Thr Leu Thr Ser Val Val Leu Ala Ala

1 5 10 15

Ser Leu Ala Leu Thr Ala Cys Ala Ser Trp Asp Ser Pro Thr Ala Ser

20 25 30

Ser Asn Gly Asp Leu Ile Glu Glu Ile Gln Ala Ser Ser Thr Ser Thr

35 40 45

Asp Pro Arg Thr Phe Thr Gly Leu Ser Ile Val Glu Asp Ile Gly Asp

50 55 60

Val Val Pro Val Thr Asp Asn Ala Ser Pro Ala Leu Pro Val Ser Leu

65 70 75 80

Thr Asp Ala Asp Gly Asn Asp Val Val Val Glu Asp Val Ser Arg Ile

85 90 95

Leu Pro Leu Asp Leu Tyr Gly Thr Tyr Ser Lys Thr Ile Ala Gly Leu

100 105 110

Gly Leu Val Asp Asn Ile Val Gly Arg Thr Val Ser Ser Thr Glu Pro

115 120 125

Ala Leu Ala Asp Thr Glu Val Val Thr Thr Gly Gly His Thr Leu Asn

130 135 140

Ala Glu Ala Ile Leu Asn Leu His Pro Thr Leu Val Ile Ile Asp His

145 150 155 160

Ser Ile Gly Pro Arg Glu Val Ile Asp Gln Ile Arg Ala Ala Gly Val

165 170 175

Ala Thr Val Ile Met Ser Pro Gln Arg Ser Ile Ala Ser Ile Gly Asp

180 185 190

Asp Ile Arg Asp Ile Ala Ser Val Val Gly Leu Pro Glu Glu Gly Glu

195 200 205

Lys Leu Ala Glu Arg Ser Val Ala Glu Val Glu Glu Ala Ser Thr Val

210 215 220

Val Asp Glu Leu Thr Pro Glu Asp Pro Leu Lys Met Val Phe Leu Tyr

225 230 235 240

Ala Arg Gly Thr Gly Gly Val Phe Phe Ile Leu Gly Asp Ala Tyr Gly

245 250 255

Gly Arg Asp Leu Ile Glu Gly Leu Gly Gly Val Asp Met Ala Ala Glu

260 265 270

Lys Gly Ile Met Asp Leu Ala Pro Ala Asn Ala Glu Ala Leu Ala Glu

275 280 285

Leu Asn Pro Asp Val Phe Val Met Met Ser Glu Gly Leu Val Ser Thr

290 295 300

Gly Gly Ile Asp Gly Leu Met Glu Arg Pro Gly Ile Ala Gln Thr Thr

305 310 315 320

Ala Gly Gln Asn Gln Arg Val Leu Ala Leu Pro Asp Gly Gln Ser Leu

325 330 335

Ala Phe Gly Ala Gln Thr Gly Glu Leu Leu Leu Arg Ala Ser Arg Glu

340 345 350

Leu Tyr Val Gln Gly Gly Glu

355

<210>77

<211>1582

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1552)

<223>RXA01850

<400>77

caaaccggcc atcgcccttt ggaatcggtc cgaaaattgc aggtacagag ccttttaccg 60

agaaaatcca ccacagattg ctgaaatttc gtgatctgtg gtg gat tcg tgc aac 115

Val Asp Ser Cys Asn

1 5

ttc aga ctc tta cgg agg cga tgg acc aaa aac aac tac aat caa gca 163

Phe Arg Leu Leu Arg Arg Arg Trp Thr Lys Asn Asn Tyr Asn Gln Ala

10 15 20

gat cac ctt gta cac cac cat aga aaa ggc cca ccc tca gcc atg gct 211

Asp His Leu Val His His His Arg Lys Gly Pro Pro Ser Ala Met Ala

25 30 35

atc agt gtt gtt gat cta ttt agc atc ggt atc gga cca tca tcc tca 259

Ile Ser Val Val Asp Leu Phe Ser Ile Gly Ile Gly Pro Ser Ser Ser

40 45 50

cat acc gtc ggc ccc atg aga gcc gcc ctc acg tat atc tct gaa ttt 307

His Thr Val Gly Pro Met Arg Ala Ala Leu Thr Tyr Ile Ser Glu Phe

55 60 65

ccc agc tcg cat gtc gat atc acg ttg cac gga tcc ctt gcc gcc acc 355

Pro Ser Ser His Val Asp Ile Thr Leu His Gly Ser Leu Ala Ala Thr

70 75 80 85

ggt aaa ggc cac tgc act gac cgg gcg gta tta ctg ggt ctg gtg gga 403

Gly Lys Gly His Cys Thr Asp Arg Ala Val Leu Leu Gly Leu Val Gly

90 95 100

tgg gaa cca acg ata gtt ccc att gat gct gca ccc tca ccc ggc gcg 451

Trp Glu Pro Thr Ile Val Pro Ile Asp Ala Ala Pro Ser Pro Gly Ala

105 110 115

ccg att cct gcg aaa ggt tct gtg aac ggg cca aag gga acg gtg tcg 499

Pro Ile Pro Ala Lys Gly Ser Val Asn Gly Pro Lys Gly Thr Val Ser

120 125 130

tat tcc ctg acg ttt gat cct cat cct ctt cca gaa cac ccc aat gcc 547

Tyr Ser Leu Thr Phe Asp Pro His Pro Leu Pro Glu His Pro Asn Ala

135 140 145

gtt acc ttt aaa gga tca acc aca agg act tat ttg tcg gtg ggt ggt 595

Val Thr Phe Lys Gly Ser Thr Thr Arg Thr Tyr Leu Ser Val Gly Gly

150 155 160 165

ggg ttc att atg acg ttg gag gat ttc cgg aag ctg gac gat atc gga 643

Gly Phe Ile Met Thr Leu Glu Asp Phe Arg Lys Leu Asp Asp Ile Gly

170 175 180

tca ggt gtg tca acc att cat cca gag gca gag gtg cct tgt cct ttt 691

Ser Gly Val Ser Thr Ile His Pro Glu Ala Glu Val Pro Cys Pro Phe

185 190 195

cag aag agt tcc caa tta ctc gca tat ggt cgc gat ttt gcg gag gtc 739

Gln Lys Ser Ser Gln Leu Leu Ala Tyr Gly Arg Asp Phe Ala Glu Val

200 205 210

atg aag gat aat gag cgc tta atc cac ggg gat ctt ggc aca gtg gat 787

Met Lys Asp Asn Glu Arg Leu Ile His Gly Asp Leu Gly Thr Val Asp

215 220 225

gcc cat ttg gat cga gtg tgg cag att atg cag gag tgc gtg gca caa 835

Ala His Leu Asp Arg Val Trp Gln Ile Met Gln Glu Cys Val Ala Gln

230 235 240 245

ggc atc gca acg ccg ggg att tta ccg ggt ggg ttg aat gtg caa cgt 883

Gly Ile Ala Thr Pro Gly Ile Leu Pro Gly Gly Leu Asn Val Gln Arg

250 255 260

cgg gcg ccg cag gta cac gcg ctg att agc aac ggg gat acg tgt gag 931

Arg Ala Pro Gln Val His Ala Leu Ile Ser Asn Gly Asp Thr Cys Glu

265 270 275

ctg ggt gct gat ctt gat gct gtg gag tgg gtg aat ctg tac gcc ttg 979

Leu Gly Ala Asp Leu Asp Ala Val Glu Trp Val Asn Leu Tyr Ala Leu

280 285 290

gcg gtg aat gaa gaa aac gcc gct ggt ggt cgt gtg gtt act gct ccg 1027

Ala Val Asn Glu Glu Asn Ala Ala Gly Gly Arg Val Val Thr Ala Pro

295 300 305

act aat ggt gct gcg ggg att att ccg gcg gtg atg cac tat gcg cgg 1075

Thr Asn Gly Ala Ala Gly Ile Ile Pro Ala Val Met His Tyr Ala Arg

310 315 320 325

gat ttt ttg aca ggt ttt ggg gcg gag cag gcg cgg acg ttt ttg tat 1123

Asp Phe Leu Thr Gly Phe Gly Ala Glu Gln Ala Arg Thr Phe Leu Tyr

330 335 340

acc gcg ggt gcg gtg ggc atc atc att aag gaa aat gcc tcg atc tct 1171

Thr Ala Gly Ala Val Gly Ile Ile Ile Lys Glu Asn Ala Ser Ile Ser

345 350 355

ggc gcg gag gtg ggg tgt cag ggt gag gtt ggt tca gcg tcc gcg atg 1219

Gly Ala Glu Val Gly Cys Gln Gly Glu Val Gly Ser Ala Ser Ala Met

360 365 370

gcg gct gcc ggg ttg tgt gca gtc tta ggt ggt tct ccg caa cag gtg 1267

Ala Ala Ala Gly Leu Cys Ala Val Leu Gly Gly Ser Pro Gln Gln Val

375 380 385

gaa aac gcc gcg gag att gcg ttg gag cac aat ttg gga ttg acg tgc 1315

Glu Asn Ala Ala Glu Ile Ala Leu Glu His Asn Leu Gly Leu Thr Cys

390 395 400 405

gat ccg gtg ggc ggg tta gtg cag att ccg tgt att gaa cgc aac gct 1363

Asp Pro Val Gly Gly Leu Val Gln Ile Pro Cys Ile Glu Arg Asn Ala

410 415 420

att gct gcc atg aag tcc atc aat gcg gca agg ctt gcc cgg att ggt 1411

Ile Ala Ala Met Lys Ser Ile Asn Ala Ala Arg Leu Ala Arg Ile Gly

425 430 435

gat ggc aac aat cgc gtg agt ttg gat gat gtg gtg gtc acg atg gct 1459

Asp Gly Asn Asn Arg Val Ser Leu Asp Asp Val Val Val Thr Met Ala

440 445 450

gcc acc ggc cgg gac atg ctg acc aaa tat aag gaa acg tcc ctt ggt 1507

Ala Thr Gly Arg Asp Met Leu Thr Lys Tyr Lys Glu Thr Ser Leu Gly

455 460 465

ggt ttg gca acc acc ttg ggc ttc ccg gtg tcg atg acg gag tgt 1552

Gly Leu Ala Thr Thr Leu Gly Phe Pro Val Ser Met Thr Glu Cys

470 475 480

tagcggtacg gctttaacac ggcttggatt 1582

<210>78

<211>484

<212>PRT

<213>谷氨酸棒状杆菌

<400>78

Val Asp Ser Cys Asn Phe Arg Leu Leu Arg Arg Arg Trp Thr Lys Asn

1 5 10 15

Asn Tyr Asn Gln Ala Asp His Leu Val His His His Arg Lys Gly Pro

20 25 30

Pro Ser Ala Met Ala Ile Ser Val Val Asp Leu Phe Ser Ile Gly Ile

35 40 45

Gly Pro Ser Ser Ser His Thr Val Gly Pro Met Arg Ala Ala Leu Thr

50 55 60

Tyr Ile Ser Glu Phe Pro Ser Ser His Val Asp Ile Thr Leu His Gly

65 70 75 80

Ser Leu Ala Ala Thr Gly Lys Gly His Cys Thr Asp Arg Ala Val Leu

85 90 95

Leu Gly Leu Val Gly Trp Glu Pro Thr Ile Val Pro Ile Asp Ala Ala

100 105 110

Pro Ser Pro Gly Ala Pro Ile Pro Ala Lys Gly Ser Val Asn Gly Pro

115 120 125

Lys Gly Thr Val Ser Tyr Ser Leu Thr Phe Asp Pro His Pro Leu Pro

130 135 140

Glu His Pro Asn Ala Val Thr Phe Lys Gly Ser Thr Thr Arg Thr Tyr

145 150 155 160

Leu Ser Val Gly Gly Gly Phe Ile Met Thr Leu Glu Asp Phe Arg Lys

165 170 175

Leu Asp Asp Ile Gly Ser Gly Val Ser Thr Ile His Pro Glu Ala Glu

180 185 190

Val Pro Cys Pro Phe Gln Lys Ser Ser Gln Leu Leu Ala Tyr Gly Arg

195 200 205

Asp Phe Ala Glu Val Met Lys Asp Asn Glu Arg Leu Ile His Gly Asp

210 215 220

Leu Gly Thr Val Asp Ala His Leu Asp Arg Val Trp Gln Ile Met Gln

225 230 235 240

Glu Cys Val Ala Gln Gly Ile Ala Thr Pro Gly Ile Leu Pro Gly Gly

245 250 255

Leu Asn Val Gln Arg Arg Ala Pro Gln Val His Ala Leu Ile Ser Asn

260 265 270

Gly Asp Thr Cys Glu Leu Gly Ala Asp Leu Asp Ala Val Glu Trp Val

275 280 285

Asn Leu Tyr Ala Leu Ala Val Asn Glu Glu Asn Ala Ala Gly Gly Arg

290 295 300

Val Val Thr Ala Pro Thr Asn Gly Ala Ala Gly Ile Ile Pro Ala Val

305 310 315 320

Met His Tyr Ala Arg Asp Phe Leu Thr Gly Phe Gly Ala Glu Gln Ala

325 330 335

Arg Thr Phe Leu Tyr Thr Ala Gly Ala Val Gly Ile Ile Ile Lys Glu

340 345 350

Asn Ala Ser Ile Ser Gly Ala Glu Val Gly Cys Gln Gly Glu Val Gly

355 360 365

Ser Ala Ser Ala Met Ala Ala Ala Gly Leu Cys Ala Val Leu Gly Gly

370 375 380

Ser Pro Gln Gln Val Glu Asn Ala Ala Glu Ile Ala Leu Glu His Asn

385 390 395 400

Leu Gly Leu Thr Cys Asp Pro Val Gly Gly Leu Val Gln Ile Pro Cys

405 410 415

Ile Glu Arg Asn Ala Ile Ala Ala Met Lys Ser Ile Asn Ala Ala Arg

420 425 430

Leu Ala Arg Ile Gly Asp Gly Asn Asn Arg Val Ser Leu Asp Asp Val

435 440 445

Val Val Thr Met Ala Ala Thr Gly Arg Asp Met Leu Thr Lys Tyr Lys

450 455 460

Glu Thr Ser Leu Gly Gly Leu Ala Thr Thr Leu Gly Phe Pro Val Ser

465 470 475 480

Met Thr Glu Cys

<210>79

<211>1066

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1036)

<223>RXA01940

<400>79

ccagaatcaa tcaccgatac agtggcagcc aaagtccagg ctgaagaagc cgctcaagcc 60

gccagctaaa tccacaaact gaactaagga gttttaccct atg acc acc aag atc 115

Met Thr Thr Lys Ile

1 5

atc ctc gac tgc gat cca gga cac gac gac gct gta gcc atg ctg ctc 163

Ile Leu Asp Cys Asp Pro Gly His Asp Asp Ala Val Ala Met Leu Leu

10 15 20

gca gcc ggc agc cca gaa att gaa ctg ctt gga atc acc acg gtc ggc 211

Ala Ala Gly Ser Pro Glu Ile Glu Leu Leu Gly Ile Thr Thr Val Gly

25 30 35

ggc aac cag acc ttg gac aag gtc acc cac aat acg cag gtc gta gcc 259

Gly Asn Gln Thr Leu Asp Lys Val Thr His Asn Thr Gln Val Val Ala

40 45 50

acc atc gct gat atc aat gcg ccc atc tac cgc ggt gtc acc cga cca 307

Thr Ile Ala Asp Ile Asn Ala Pro Ile Tyr Arg Gly Val Thr Arg Pro

55 60 65

ttg gtg cgc ccc gtt gag gta gcc gaa gat atc cac ggc gat acc ggc 355

Leu Val Arg Pro Val Glu Val Ala Glu Asp Ile His Gly Asp Thr Gly

70 75 80 85

atg gaa atc cac aag tac gaa ctg cct gaa cca acc aag cag gta gaa 403

Met Glu Ile His Lys Tyr Glu Leu Pro Glu Pro Thr Lys Gln Val Glu

90 95 100

gac acc cac gcg gtg gat ttc atc atc gat acc atc atg aat aac gag 451

Asp Thr His Ala Val Asp Phe Ile Ile Asp Thr Ile Met Asn Asn Glu

105 110 115

ccc ggc agc gta gcg ctg gtt ccc acc gga cca ctg acc aac atc gcg 499

Pro Gly Ser Val Ala Leu Val Pro Thr Gly Pro Leu Thr Asn Ile Ala

120 125 130

ctg gca gtc cgg aaa gaa cca cgc atc gcc gag cga gtc aag gaa gtt 547

Leu Ala Val Arg Lys Glu Pro Arg Ile Ala Glu Arg Val Lys Glu Val

135 140 145

gtc ctc atg ggc ggg ggc tac cac gta gga aac tgg acc gcc gta gct 595

Val Leu Met Gly Gly Gly Tyr His Val Gly Asn Trp Thr Ala Val Ala

150 155 160 165

gaa ttc aac atc aag atc gac ccc gaa gca gcc cac atc gta ttc aac 643

Glu Phe Asn Ile Lys Ile Asp Pro Glu Ala Ala His Ile Val Phe Asn

170 175 180

gaa aag tgg cca ctg act atg gtc ggc ctc gac ctt acc cac cag gcg 691

Glu Lys Trp Pro Leu Thr Met Val Gly Leu Asp Leu Thr His Gln Ala

185 190 195

ctc gca aca cct gag atc gaa gcc aag ttc aac gag ctg ggc acc gac 739

Leu Ala Thr Pro Glu Ile Glu Ala Lys Phe Asn Glu Leu Gly Thr Asp

200 205 210

gtc gcc gac ttc gtc gtc gcg ctt ttc gac gct ttc cgc aag aat tac 787

Val Ala Asp Phe Val Val Ala Leu Phe Asp Ala Phe Arg Lys Asn Tyr

215 220 225

cag gac gca cag ggt ttt gat aac cca cca gta cac gac cct tgt gct 835

Gln Asp Ala Gln Gly Phe Asp Asn Pro Pro Val His Asp Pro Cys Ala

230 235 240 245

gtt gca tac ctt gtt gac cca acc gta ttc acc acc cgc aaa gca cca 883

Val Ala Tyr Leu Val Asp Pro Thr Val Phe Thr Thr Arg Lys Ala Pro

250 255 260

ctc gat gtg gag ctg tac ggc gca ctc acc aca ggc atg acc gtt gct 931

Leu Asp Val Glu Leu Tyr Gly Ala Leu Thr Thr Gly Met Thr Val Ala

265 270 275

gat ttc cgc gca ccg gct cca gca gat tgc acc acc caa gta gct gtt 979

Asp Phe Arg Ala Pro Ala Pro Ala Asp Cys Thr Thr Gln Val Ala Val

280 285 290

gac ctg gac ttt gat aaa ttc tgg aac atg gtg atc gat gca gta aag 1027

Asp Leu Asp Phe Asp Lys Phe Trp Asn Met Val Ile Asp Ala Val Lys

295 300 305

cgc atc gga tagacctgtt cacaaggttg ttagagggcg 1066

Arg Ile Gly

310

<210>80

<211>312

<212>PRT

<213>谷氨酸棒状杆菌

<400>80

Met Thr Thr Lys Ile Ile Leu Asp Cys Asp Pro Gly His Asp Asp Ala

1 5 10 15

Val Ala Met Leu Leu Ala Ala Gly Ser Pro Glu Ile Glu Leu Leu Gly

20 25 30

Ile Thr Thr Val Gly Gly Asn Gln Thr Leu Asp Lys Val Thr His Asn

35 40 45

Thr Gln Val Val Ala Thr Ile Ala Asp Ile Asn Ala Pro Ile Tyr Arg

50 55 60

Gly Val Thr Arg Pro Leu Val Arg Pro Val Glu Val Ala Glu Asp Ile

65 70 75 80

His Gly Asp Thr Gly Met Glu Ile His Lys Tyr Glu Leu Pro Glu Pro

85 90 95

Thr Lys Gln Val Glu Asp Thr His Ala Val Asp Phe Ile Ile Asp Thr

100 105 110

Ile Met Asn Asn Glu Pro Gly Ser Val Ala Leu Val Pro Thr Gly Pro

115 120 125

Leu Thr Asn Ile Ala Leu Ala Val Arg Lys Glu Pro Arg Ile Ala Glu

130 135 140

Arg Val Lys Glu Val Val Leu Met Gly Gly Gly Tyr His Val Gly Asn

145 150 155 160

Trp Thr Ala Val Ala Glu Phe Asn Ile Lys Ile Asp Pro Glu Ala Ala

165 170 175

His Ile Val Phe Asn Glu Lys Trp Pro Leu Thr Met Val Gly Leu Asp

180 185 190

Leu Thr His Gln Ala Leu Ala Thr Pro Glu Ile Glu Ala Lys Phe Asn

195 200 205

Glu Leu Gly Thr Asp Val Ala Asp Phe Val Val Ala Leu Phe Asp Ala

210 215 220

Phe Arg Lys Asn Tyr Gln Asp Ala Gln Gly Phe Asp Asn Pro Pro Val

225 230 235 240

His Asp Pro Cys Ala Val Ala Tyr Leu Val Asp Pro Thr Val Phe Thr

245 250 255

Thr Arg Lys Ala Pro Leu Asp Val Glu Leu Tyr Gly Ala Leu Thr Thr

260 265 270

Gly Met Thr Val Ala Asp Phe Arg Ala Pro Ala Pro Ala Asp Cys Thr

275 280 285

Thr Gln Val Ala Val Asp Leu Asp Phe Asp Lys Phe Trp Asn Met Val

290 295 300

Ile Asp Ala Val Lys Arg Ile Gly

305 310

<210>81

<211>1078

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1048)

<223>RXA02021

<400>81

ttgggtcgcc gaggagatct aatcctggtt tgagttcaga gttcacaggt ttaagcctac 60

aaaccttagt taaaacatga tggaagcggt cgattaaaaa atg agt gaa aac att 115

Met Ser Glu Asn Ile

1 5

cgc gga gcc caa gca gtt gga atc gca aat atc gcc atg gac ggg acc 163

Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile Ala Met Asp Gly Thr

10 15 20

atc ctg gac acg tgg tac cca gaa ccc caa att ttc aac ccg gat cag 211

Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile Phe Asn Pro Asp Gln

25 30 35

tgg gct gaa cgc tac cca ttg gaa gtg ggc acc aca cgc ctc gga gca 259

Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr Thr Arg Leu Gly Ala

40 45 50

aac gaa ctc acc cca cgg atg ctg cag ttg gta aaa ctg gac caa gat 307

Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val Lys Leu Asp Gln Asp

55 60 65

cgc ctc gtc gaa cag gta gca gtc cgc acc gtt atc ccc gat ctg tct 355

Arg Leu Val Glu Gln Val Ala Val Arg Thr Val Ile Pro Asp Leu Ser

70 75 80 85

caa cct cca gta gac gcg cac gat gtt tac ctg cgc ctc cac ctg ctt 403

Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu Arg Leu His Leu Leu

90 95 100

tcc cac cgc ctg gtc cgc ccc cac gaa atg cac atg caa aac acc ttg 451

Ser His Arg Leu Val Arg Pro His Glu Met His Met Gln Asn Thr Leu

105 110 115

gag ctg ctg tcc gac gtg gtg tgg aca aac aag ggc cct tgc ctt cct 499

Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys Gly Pro Cys Leu Pro

120 125 130

gaa aac ttt gag tgg gtg cgt ggt gct ctg cgg tcc cgc gga ctc atc 547

Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg Ser Arg Gly Leu Ile

135 140 145

cac gtc tac tgt gtg gac cgt ctt ccc cgc atg gtc gac tat gtg gtt 595

His Val Tyr Cys Val Asp Arg Leu Pro Arg Met Val Asp Tyr Val Val

150 155 160 165

ccc cct gga gtc cgc atc tcc gaa gca gaa cgc gtg cgc cta ggt gca 643

Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg Val Arg Leu Gly Ala

170 175 180

tac ctt gct ccg ggt acc tct gtg ctg cgt gaa ggt ttc gtg tct ttc 691

Tyr Leu Ala Pro G1y Thr Ser Val Leu Arg Glu Gly Phe Val Ser Phe

185 190 195

aac tcc ggc acc ttg ggt gcc gca aag gtg gaa ggc cgc ctg agt tcc 739

Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu Gly Arg Leu Ser Ser

200 205 210

ggt gtg gtc atc ggt gaa ggt tcc gag att gga ctg tct tct act att 787

Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly Leu Ser Ser Thr Ile

215 220 225

cag tcc ccg aga gat gaa cag cgc cgc cgt ttg ccg ttg agc atc ggc 835

Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu Pro Leu Ser Ile Gly

230 235 240 245

caa aac tgc aac ttt ggt gtc agc tcc gga atc atc gga gtc agt ctg 883

Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile Ile Gly Val Ser Leu

250 255 260

gga gac aat tgc gac atc gga aat aac att gtc ttg gat gga gat acc 931

Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val Leu Asp Gly Asp Thr

265 270 275

ccc att tgg ttc gca gcc gat gag gag tta cgc act atc gac tcc atc 979

Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg Thr Ile Asp Ser Ile

280 285 290

gaa ggc caa gca aat tgg tca atc aag cgt gaa tcc ggc ttc cat gag 1027

Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu Ser Gly Phe His Glu

295 300 305

cca gtt gcc cgc ctc aaa gct tgacccattt tcataaccag tgccatgtgg 1078

Pro Val Ala Arg Leu Lys Ala

310 315

<210>82

<211>316

<212>PRT

<213>谷氨酸棒状杆菌

<400>82

Met Ser Glu Asn Ile Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile

1 5 10 15

Ala Met Asp Gly Thr Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile

20 25 30

Phe Asn Pro Asp Gln Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr

35 40 45

Thr Arg Leu Gly Ala Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val

50 55 60

Lys Leu Asp Gln Asp Arg Leu Val Glu Gln Val Ala Val Arg Thr Val

65 70 75 80

Ile Pro Asp Leu Ser Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu

85 90 95

Arg Leu His Leu Leu Ser His Arg Leu Val Arg Pro His Glu Met His

100 105 110

Met Gln Asn Thr Leu Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys

115 120 125

Gly Pro Cys Leu Pro Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg

130 135 140

Ser Arg Gly Leu Ile His Val Tyr Cys Val Asp Arg Leu Pro Arg Met

145 150 155 160

Val Asp Tyr Val Val Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg

165 170 175

Val Arg Leu Gly Ala Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu

180 185 190

Gly Phe Val Ser Phe Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu

195 200 205

Gly Arg Leu Ser Ser Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly

210 215 220

Leu Ser Ser Thr Ile Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu

225 230 235 240

Pro Leu Ser Ile Gly Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile

245 250 255

Ile Gly Val Ser Leu Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val

260 265 270

Leu Asp Gly Asp Thr Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg

275 280 285

Thr Ile Asp Ser Ile Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu

290 295 300

Ser Gly Phe His Glu Pro Val Ala Arg Leu Lys Ala

305 310 315

<210>83

<211>3871

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3841)

<223>RXA02095

<400>83

ctctcttggt cctctcccca cccattttta agtactcaag acccttccaa cagaaaggat 60

tactccccca acaggctcaa aaatactgaa aggctcacgc atg aaa act gag caa 115

Met Lys Thr Glu Gln

1 5

tcc caa aaa gca caa tta gcc cct aag aaa gca cct gaa aag cca caa 163

Ser Gln Lys Ala Gln Leu Ala Pro Lys Lys Ala Pro Glu Lys Pro Gln

10 15 20

cgc atc cgc caa ctt att tcc gtg gcg tgg cag cga cct tgg ctc acc 211

Arg Ile Arg Gln Leu Ile Ser Val Ala Trp Gln Arg Pro Trp Leu Thr

25 30 35

tca ttc acc gta atc agc gct tta gct gca acg ttg ttt gaa ctt aca 259

Ser Phe Thr Val Ile Ser Ala Leu Ala Ala Thr Leu Phe Glu Leu Thr

40 45 50

ctt cct ctt ttg acc ggt ggc gcc atc gat atc gcg ctc gga aat acc 307

Leu Pro Leu Leu Thr Gly Gly Ala Ile Asp Ile Ala Leu Gly Asn Thr

55 60 65

gga gat act tta acc act gac ctg ctg gac cgg ttc act ccg agt gga 355

Gly Asp Thr Leu Thr Thr Asp Leu Leu Asp Arg Phe Thr Pro Ser Gly

70 75 80 85

tta agc gtg ttg acc agc gtc att gcc ctt atc gtg ctt ctc gcg ttg 403

Leu Ser Val Leu Thr Ser Val Ile Ala Leu Ile Val Leu Leu Ala Leu

90 95 100

ctt cgc tat gcc agt caa ttt gga cgg cga tac acc gca ggc aag ctc 451

Leu Arg Tyr Ala Ser Gln Phe Gly Arg Arg Tyr Thr Ala Gly Lys Leu

105 110 115

agc atg ggg gta cag cat gat gtc cgg ctt aaa acg atg cgc tca ttg 499

Ser Met Gly Val Gln His Asp Val Arg Leu Lys Thr Met Arg Ser Leu

120 125 130

cag aac ctc gat ggg cca ggt cag gac tct att cgc aca ggc caa gta 547

Gln Asn Leu Asp Gly Pro Gly Gln Asp Ser Ile Arg Thr Gly Gln Val

135 140 145

gtc agt cgg tcc att tcg gat atc aac atg gtg caa agc ctt gtg gcg 595

Val Ser Arg Ser Ile Ser Asp Ile Asn Met Val Gln Ser Leu Val Ala

150 155 160 165

atg ttg ccg atg ttg atc gga aat gtg gtc aag ctt gtg ctc act ttg 643

Met Leu Pro Met Leu Ile Gly Asn Val Val Lys Leu Val Leu Thr Leu

170 175 180

gtg atc atg ctg gct att tcc ccg ccg ctg acc atc atc gct gca gtg 691

Val Ile Met Leu Ala Ile Ser Pro Pro Leu Thr Ile Ile Ala Ala Val

185 190 195

ttg gtg cct ttg ctg ttg tgg gcc gtg gcc tat tcg cga aaa gcg ctt 739

Leu Val Pro Leu Leu Leu Trp Ala Val Ala Tyr Ser Arg Lys Ala Leu

200 205 210

ttt gcg tcc acg tgg tcg gcc cag caa aag gct gcg gat ctg acc act 787

Phe Ala Ser Thr Trp Ser Ala Gln Gln Lys Ala Ala Asp Leu Thr Thr

215 220 225

cat gtg gaa gaa act gtc acg ggt atc cgc gtg gtc aag gca ttt gcg 835

His Val Glu Glu Thr Val Thr Gly Ile Arg Val Val Lys Ala Phe Ala

230 235 240 245

cag gaa gac cgc gag acc gac aaa ttg gat ctc acc gca cgt gag tta 883

Gln Glu Asp Arg Glu Thr Asp Lys Leu Asp Leu Thr Ala Arg Glu Leu

250 255 260

ttt gcc cag cgc atg cgc act gca cgt ctg acg gca aag ttc atc ccc 931

Phe Ala Gln Arg Met Arg Thr Ala Arg Leu Thr Ala Lys Phe Ile Pro

265 270 275

atg gtt gag cag ctt ccg cag ctt gct ttg gtg gtc aac att gtt ggc 979

Met Val Glu Gln Leu Pro Gln Leu Ala Leu Val Val Asn Ile Val Gly

280 285 290

ggt ggc tat ttg gcc atg act ggt cac atc acg gtg ggc acg ttt gtg 1027

Gly Gly Tyr Leu Ala Met Thr Gly His Ile Thr Val Gly Thr Phe Val

295 300 305

gcg ttt tct tcc tat ctc act agc ttg tcg gcg gtg gct agg tcc ctg 1075

Ala Phe Ser Ser Tyr Leu Thr Ser Leu Ser Ala Val Ala Arg Ser Leu

310 315 320 325

tcg ggc atg ctc atg cgc gtg cag ttg gcg ctg tct tct gtg gag cgc 1123

Ser Gly Met Leu Met Arg Val Gln Leu Ala Leu Ser Ser Val Glu Arg

330 335 340

atc ttt gaa gtc att gat ctt cag cct gaa cgc acc gat cct gca cac 1171

Ile Phe Glu Val Ile Asp Leu Gln Pro Glu Arg Thr Asp Pro Ala His

345 350 355

ccc ctg tca ctt ccc gac act ccc ctg ggt ctg tcg ttc aac aac gta 1219

Pro Leu Ser Leu Pro Asp Thr Pro Leu Gly Leu Ser Phe Asn Asn Val

360 365 370

gat ttc cgt ggg att ctc aac ggt ttt gag ctg ggt gtt cag gcc ggt 1267

Asp Phe Arg Gly Ile Leu Asn Gly Phe Glu Leu Gly Val Gln Ala Gly

375 380 385

gaa acc gtt gtg ttg gtg ggc cct cca ggt tca ggc aag acc atg gct 1315

Glu Thr Val Val Leu Val Gly Pro Pro Gly Ser Gly Lys Thr Met Ala

390 395 400 405

gtg cag ctt gct gga aac ttt tat caa cca gac agc ggc cac atc gcc 1363

Val Gln Leu Ala Gly Asn Phe Tyr Gln Pro Asp Ser Gly His Ile Ala

410 415 420

ttt gat agc aac ggc cat cgc act cgc ttc gac gac ctc acc cac agc 1411

Phe Asp Ser Asn Gly His Arg Thr Arg Phe Asp Asp Leu Thr His Ser

425 430 435

gat atc cgc agg aat ctc atc gcg gtt ttt gat gag ccg ttc ttg tac 1459

Asp Ile Arg Arg Asn Leu Ile Ala Val Phe Asp Glu Pro Phe Leu Tyr

440 445 450

tcc tcc tcc atc cgc gag aac atc tcg atg ggt ttg gat gtc agt gat 1507

Ser Ser Ser Ile Arg Glu Asn Ile Ser Met Gly Leu Asp Val Ser Asp

455 460 465

gag cag atc gaa cac gca gcc agg ctt gcc cag gct cat gat ttt atc 1555

Glu Gln Ile Glu His Ala Ala Arg Leu Ala Gln Ala His Asp Phe Ile

470 475 480 485

gat cgc ctt cca aac aaa tac gag gaa gtc att ggc gaa cgc ggc ctg 1603

Asp Arg Leu Pro Asn Lys Tyr Glu Glu Val Ile Gly Glu Arg Gly Leu

490 495 500

acg ctt tct ggt ggt caa cgc caa cgc atc gcc ctc gca cgg gct ttc 1651

Thr Leu Ser Gly Gly Gln Arg Gln Arg Ile Ala Leu Ala Arg Ala Phe

505 510 515

ctg gcg cat ccc aaa gtg ttg gtg ctt gat gat gcc acc tct gcc att 1699

Leu Ala His Pro Lys Val Leu Val Leu Asp Asp Ala Thr Ser Ala Ile

520 525 530

gat gcc tcc act gag gac cgc att ttc cag gcc ttg cgc gaa gaa ctg 1747

Asp Ala Ser Thr Glu Asp Arg Ile Phe Gln Ala Leu Arg Glu Glu Leu

535 540 545

cac gat gtc acc att ttg atc atc gcg cac cgc cac tcc act ttg gag 1795

His Asp Val Thr Ile Leu Ile Ile Ala His Arg His Ser Thr Leu Glu

550 555 560 565

ctc ggc gat cgg gtt ggt ctg gtc gaa gat gga cgg gta aca gca ctg 1843

Leu Gly Asp Arg Val Gly Leu Val Glu Asp Gly Arg Val Thr Ala Leu

570 575 580

gga ccg ttg agt gag atg cgt gat cac gct cgt ttc tcg cat ctg atg 1891

Gly Pro Leu Ser Glu Met Arg Asp His Ala Arg Phe Ser His Leu Met

585 590 595

gct ctt gat ttc cag gat tct cac gat ccg gaa ttc acc ctc gac aac 1939

Ala Leu Asp Phe Gln Asp Ser His Asp Pro Glu Phe Thr Leu Asp Asn

600 605 610

ggt tca cta ccc agc caa gag caa ttg tgg ccg gag gtc tcc aca gaa 1987

Gly Ser Leu Pro Ser Gln Glu Gln Leu Trp Pro Glu Val Ser Thr Glu

615 620 625

aag cag tac aag att ctt gcg cct gcc cct ggt cga ggc cgt ggc atg 2035

Lys Gln Tyr Lys Ile Leu Ala Pro Ala Pro Gly Arg Gly Arg Gly Met

630 635 640 645

tcc atg cca gca acc cct gag ctg ctc gcc cag att gag gcg ctg cca 2083

Ser Met Pro Ala Thr Pro Glu Leu Leu Ala Gln Ile Glu Ala Leu Pro

650 655 660

gca gca acg gaa gaa aca cga gtt gat gcc ggg agg cta cgc acc agt 2131

Ala Ala Thr Glu Glu Thr Arg Val Asp Ala Gly Arg Leu Arg Thr Ser

665 670 675

acc tcc ggt ttc aaa ttg ctc agt tta ttc aag cag gtc cgt tgg ctc 2179

Thr Ser Gly Phe Lys Leu Leu Ser Leu Phe Lys Gln Val Arg Trp Leu

680 685 690

gtc gtc gcg gtc atc gcg ttg ttg ctg gtg ggc gta gcc gcc gat cta 2227

Val Val Ala Val Ile Ala Leu Leu Leu Val Gly Val Ala Ala Asp Leu

695 700 705

gca ttt cca aca ctg atg cgc gca gcc atc gac aac ggt gtg caa gca 2275

Ala Phe Pro Thr Leu Met Arg Ala Ala Ile Asp Asn Gly Val Gln Ala

710 715 720 725

caa agc acc tcc acg ttg tgg tgg atc gcc atc gca ggc agc gta gta 2323

Gln Ser Thr Ser Thr Leu Trp Trp Ile Ala Ile Ala Gly Ser Val Val

730 735 740

gtc ctt ctg tcc tgg gcc gcc gcc gcg atc aac acg att atc acg gca 2371

Val Leu Leu Ser Trp Ala Ala Ala Ala Ile Asn Thr Ile Ile Thr Ala

745 750 755

cgc acc ggt gaa cgg ctg ctt tac ggc ttg cgt ctg cgc tca ttt gtg 2419

Arg Thr Gly Glu Arg Leu Leu Tyr Gly Leu Arg Leu Arg Ser Phe Val

760 765 770

cat cta ttg cgc ctg tcc atg agc tat ttc gaa cgc acc atg tcc ggc 2467

His Leu Leu Arg Leu Ser Met Ser Tyr Phe Glu Arg Thr Met Ser Gly

775 780 785

cgc atc atg acg cgc atg acc acc gac atc gac aac ctc tcg tcc ttc 2515

Arg Ile Met Thr Arg Met Thr Thr Asp Ile Asp Asn Leu Ser Ser Phe

790 795 800 805

ctc caa tca ggt ctg gcg caa aca gtt gtc tct gtg ggc acg ctc atc 2563

Leu Gln Ser Gly Leu Ala Gln Thr Val Val Ser Val Gly Thr Leu Ile

810 815 820

ggt gtg gtc acc atg ctc gcc atc acc gac gca caa cta gca ctc gtt 2611

Gly Val Val Thr Met Leu Ala Ile Thr Asp Ala Gln Leu Ala Leu Val

825 830 835

gcg ctg tcc gtg gtg ccg atc atc atc gtg ctc act ctc att ttc cga 2659

Ala Leu Ser Val Val Pro Ile Ile Ile Val Leu Thr Leu Ile Phe Arg

840 845 850

cgc atc agc tcc agg ctg tac acc gct tca cgc gag caa gcc agc cag 2707

Arg Ile Ser Ser Arg Leu Tyr Thr Ala Ser Arg Glu Gln Ala Ser Gln

855 860 865

gtc aac gcg gta ttc cac gag tcc atc gcc ggt tta cgc acc gcg cag 2755

Val Asn Ala Val Phe His Glu Ser Ile Ala Gly Leu Arg Thr Ala Gln

870 875 880 885

atg cac cgc atg gaa gac caa gtc ttt gac aat tat gcg ggc gaa gca 2803

Met His Arg Met Glu Asp Gln Val Phe Asp Asn Tyr Ala Gly Glu Ala

890 895 900

gag gaa ttc cga cgc ctg cgt gtg aaa tcc cag acg gcc atc gcc atc 2851

Glu Glu Phe Arg Arg Leu Arg Val Lys Ser Gln Thr Ala Ile Ala Ile

905 910 915

tac ttc ccc ggc ctt ggc gcg ctc tct gaa atc gcc cag gca ctc gtc 2899

Tyr Phe Pro Gly Leu Gly Ala Leu Ser Glu Ile Ala Gln Ala Leu Val

920 925 930

ctc ggt ttc ggc gca ctg caa gta acg cgc ggc gac atc tcc acc ggc 2947

Leu Gly Phe Gly Ala Leu Gln Val Thr Arg Gly Asp Ile Ser Thr Gly

935 940 945

gta ctc gtg gca ttc gtg ctg tac atg ggc ctg atg ttc ggc ccc atc 2995

Val Leu Val Ala Phe Val Leu Tyr Met Gly Leu Met Phe Gly Pro Ile

950 955 960 965

caa caa cta agc caa atc ttc gac tcc tac caa caa gcc gcc gtc ggc 3043

Gln Gln Leu Ser Gln Ile Phe Asp Ser Tyr Gln Gln Ala Ala Val Gly

970 975 980

ttc cgt cgc atc acc gaa ctg ctc gca acg cag ccc agc gtc cca gat 3091

Phe Arg Arg Ile Thr Glu Leu Leu Ala Thr Gln Pro Ser Val Pro Asp

985 990 995

ctg ggc acc aac agg cac gct agg cag gct gcc acg cag cct tta ttg 3139

Leu Gly Thr Asn Arg His Ala Arg Gln Ala Ala Thr Gln Pro Leu Leu

1000 1005 1010

ctt gac gac gtc acc ttc ggc tat tca gac gat ccg atc cta gac aac 3187

Leu Asp Asp Val Thr Phe Gly Tyr Ser Asp Asp Pro Ile Leu Asp Asn

1015 1020 1025

gtc acc gtc cag atc gaa ccg gga acc acc gtc gct gtg gtg gga cca 3235

Val Thr Val Gln Ile Glu Pro Gly Thr Thr Val Ala Val Val Gly Pro

1030 1035 1040 1045

acc ggc gct gga aaa tcc acc gtg gtg aaa ctc ctt tcc cgc ctc tac 3283

Thr Gly Ala Gly Lys Ser Thr Val Val Lys Leu Leu Ser Arg Leu Tyr

1050 1055 1060

gac ccc aat gaa ggt gcc gtg aaa gct ggc gag atc gat atc aag gac 3331

Asp Pro Asn Glu Gly Ala Val Lys Ala Gly Glu Ile Asp Ile Lys Asp

1065 1070 1075

ttc ccc acc gct gat tgg cgc cgc aca atc ggt acc gtg cca caa gaa 3379

Phe Pro Thr Ala Asp Trp Arg Arg Thr Ile Gly Thr Val Pro Gln Glu

1080 1085 1090

gca cac ttg ttt agt gga agc atc gcc gac aac att ggc tac gga tgc 3427

Ala His Leu Phe Ser Gly Ser Ile Ala Asp Asn Ile Gly Tyr Gly Cys

1095 1100 1105

agg gag gcg tcg aca agc aaa atc gaa gcg gca gca cgc cgc gtc gga 3475

Arg Glu Ala Ser Thr Ser Lys Ile Glu Ala Ala Ala Arg Arg Val Gly

1110 1115 1120 1125

gcc tta aac gcc atc gcc gcc atc cct gat ggt ttc aac cat caa gtc 3523

Ala Leu Asn Ala Ile Ala Ala Ile Pro Asp Gly Phe Asn His Gln Val

1130 1135 1140

ggt gaa cgc ggg cgc aac ctg tca tcc gga cag cgc caa ctg atc gcg 3571

Gly Glu Arg Gly Arg Asn Leu Ser Ser Gly Gln Arg Gln Leu Ile Ala

1145 1150 1155

ctg gcg cgc gcc gaa ctt atc gag cct tcc atc atg ctt ctc gac gaa 3619

Leu Ala Arg Ala Glu Leu Ile Glu Pro Ser Ile Met Leu Leu Asp Glu

1160 1165 1170

gcc acc tcc acc ctc gac ccc gcc acc gaa gcc gtt atc ctc aac gcc 3667

Ala Thr Ser Thr Leu Asp Pro Ala Thr Glu Ala Val Ile Leu Asn Ala

1175 1180 1185

tcc gat cga gtc act aag gga cgc acc agc atc atc gtc gcg cac cgc 3715

Ser Asp Arg Val Thr Lys Gly Arg Thr Ser Ile Ile Val Ala His Arg

1190 1195 1200 1205

ttg gca acc gct aaa agg gcc gac cgt att ctt gtt gtt gaa caa gga 3763

Leu Ala Thr Ala Lys Arg Ala Asp Arg Ile Leu Val Val Glu Gln Gly

1210 1215 1220

cgt atc att gag gac gga tct cac gac gcg ttg ttg tct gct aac ggc 3811

Arg Ile Ile Glu Asp Gly Ser His Asp Ala Leu Leu Ser Ala Asn Gly

1225 1230 1235

acc tac gcc cgc atg tgg cat tta atg gcc tgacacgtta tttttaggag 3861

Thr Tyr Ala Arg Met Trp His Leu Met Ala

1240 1245

aactgtcaac 3871

<210>84

<211>1247

<212>PRT

<213>谷氨酸棒状杆菌

<400>84

Met Lys Thr Glu Gln Ser Gln Lys Ala Gln Leu Ala Pro Lys Lys Ala

1 5 10 15

Pro Glu Lys Pro Gln Arg Ile Arg Gln Leu Ile Ser Val Ala Trp Gln

20 25 30

Arg Pro Trp Leu Thr Ser Phe Thr Val Ile Ser Ala Leu Ala Ala Thr

35 40 45

Leu Phe Glu Leu Thr Leu Pro Leu Leu Thr Gly Gly Ala Ile Asp Ile

50 55 60

Ala Leu Gly Asn Thr Gly Asp Thr Leu Thr Thr Asp Leu Leu Asp Arg

65 70 75 80

Phe Thr Pro Ser Gly Leu Ser Val Leu Thr Ser Val Ile Ala Leu Ile

85 90 95

Val Leu Leu Ala Leu Leu Arg Tyr Ala Ser Gln Phe Gly Arg Arg Tyr

100 105 110

Thr Ala Gly Lys Leu Ser Met Gly Val Gln His Asp Val Arg Leu Lys

115 120 125

Thr Met Arg Ser Leu Gln Asn Leu Asp Gly Pro Gly Gln Asp Ser Ile

130 135 140

Arg Thr Gly Gln Val Val Ser Arg Ser Ile Ser Asp Ile Asn Met Val

145 150 155 160

Gln Ser Leu Val Ala Met Leu Pro Met Leu Ile Gly Asn Val Val Lys

165 170 175

Leu Val Leu Thr Leu Val Ile Met Leu Ala Ile Ser Pro Pro Leu Thr

180 185 190

Ile Ile Ala Ala Val Leu Val Pro Leu Leu Leu Trp Ala Val Ala Tyr

195 200 205

Ser Arg Lys Ala Leu Phe Ala Ser Thr Trp Ser Ala Gln Gln Lys Ala

210 215 220

Ala Asp Leu Thr Thr His Val Glu Glu Thr Val Thr Gly Ile Arg Val

225 230 235 240

Val Lys Ala Phe Ala Gln Glu Asp Arg Glu Thr Asp Lys Leu Asp Leu

245 250 255

Thr Ala Arg Glu Leu Phe Ala Gln Arg Met Arg Thr Ala Arg Leu Thr

260 265 270

Ala Lys Phe Ile Pro Met Val Glu Gln Leu Pro Gln Leu Ala Leu Val

275 280 285

Val Asn Ile Val Gly Gly Gly Tyr Leu Ala Met Thr Gly His Ile Thr

290 295 300

Val Gly Thr Phe Val Ala Phe Ser Ser Tyr Leu Thr Ser Leu Ser Ala

305 310 315 320

Val Ala Arg Ser Leu Ser Gly Met Leu Met Arg Val Gln Leu Ala Leu

325 330 335

Ser Ser Val Glu Arg Ile Phe Glu Val Ile Asp Leu Gln Pro Glu Arg

340 345 350

Thr Asp Pro Ala His Pro Leu Ser Leu Pro Asp Thr Pro Leu Gly Leu

355 360 365

Ser Phe Asn Asn Val Asp Phe Arg Gly Ile Leu Asn Gly Phe Glu Leu

370 375 380

Gly Val Gln Ala Gly Glu Thr Val Val Leu Val Gly Pro Pro Gly Ser

385 390 395 400

Gly Lys Thr Met Ala Val Gln Leu Ala Gly Asn Phe Tyr Gln Pro Asp

405 410 415

Ser Gly His Ile Ala Phe Asp Ser Asn Gly His Arg Thr Arg Phe Asp

420 425 430

Asp Leu Thr His Ser Asp Ile Arg Arg Asn Leu Ile Ala Val Phe Asp

435 440 445

Glu Pro Phe Leu Tyr Ser Ser Ser Ile Arg Glu Asn Ile Ser Met Gly

450 455 460

Leu Asp Val Ser Asp Glu Gln Ile Glu His Ala Ala Arg Leu Ala Gln

465 470 475 480

Ala His Asp Phe Ile Asp Arg Leu Pro Asn Lys Tyr Glu Glu Val Ile

485 490 495

Gly Glu Arg Gly Leu Thr Leu Ser Gly Gly Gln Arg Gln Arg Ile Ala

500 505 510

Leu Ala Arg Ala Phe Leu Ala His Pro Lys Val Leu Val Leu Asp Asp

515 520 525

Ala Thr Ser Ala Ile Asp Ala Ser Thr Glu Asp Arg Ile Phe Gln Ala

530 535 540

Leu Arg Glu Glu Leu His Asp Val Thr Ile Leu Ile Ile Ala His Arg

545 550 555 560

His Ser Thr Leu Glu Leu Gly Asp Arg Val Gly Leu Val Glu Asp Gly

565 570 575

Arg Val Thr Ala Leu Gly Pro Leu Ser Glu Met Arg Asp His Ala Arg

580 585 590

Phe Ser His Leu Met Ala Leu Asp Phe Gln Asp Ser His Asp Pro Glu

595 600 605

Phe Thr Leu Asp Asn Gly Ser Leu Pro Ser Gln Glu Gln Leu Trp Pro

610 615 620

Glu Val Ser Thr Glu Lys Gln Tyr Lys Ile Leu Ala Pro Ala Pro Gly

625 630 635 640

Arg Gly Arg Gly Met Ser Met Pro Ala Thr Pro Glu Leu Leu Ala Gln

645 650 655

Ile Glu Ala Leu Pro Ala Ala Thr Glu Glu Thr Arg Val Asp Ala Gly

660 665 670

Arg Leu Arg Thr Ser Thr Ser Gly Phe Lys Leu Leu Ser Leu Phe Lys

675 680 685

Gln Val Arg Trp Leu Val Val Ala Val Ile Ala Leu Leu Leu Val Gly

690 695 700

Val Ala Ala Asp Leu Ala Phe Pro Thr Leu Met Arg Ala Ala Ile Asp

705 710 715 720

Asn Gly Val Gln Ala Gln Ser Thr Ser Thr Leu Trp Trp Ile Ala Ile

725 730 735

Ala Gly Ser Val Val Val Leu Leu Ser Trp Ala Ala Ala Ala Ile Asn

740 745 750

Thr Ile Ile Thr Ala Arg Thr Gly Glu Arg Leu Leu Tyr Gly Leu Arg

755 760 765

Leu Arg Ser Phe Val His Leu Leu Arg Leu Ser Met Ser Tyr Phe Glu

770 775 780

Arg Thr Met Ser Gly Arg Ile Met Thr Arg Met Thr Thr Asp Ile Asp

785 790 795 800

Asn Leu Ser Ser Phe Leu Gln Ser Gly Leu Ala Gln Thr Val Val Ser

805 8l0 815

Val Gly Thr Leu Ile Gly Val Val Thr Met Leu Ala Ile Thr Asp Ala

820 825 830

Gln Leu Ala Leu Val Ala Leu Ser Val Val Pro Ile Ile Ile Val Leu

835 840 845

Thr Leu Ile Phe Arg Arg Ile Ser Ser Arg Leu Tyr Thr Ala Ser Arg

850 855 860

Glu Gln Ala Ser Gln Val Asn Ala Val Phe His Glu Ser Ile Ala Gly

865 870 875 880

Leu Arg Thr Ala Gln Met His Arg Met Glu Asp Gln Val Phe Asp Asn

885 890 895

Tyr Ala Gly Glu Ala Glu Glu Phe Arg Arg Leu Arg Val Lys Ser Gln

900 905 910

Thr Ala Ile Ala Ile Tyr Phe Pro Gly Leu Gly Ala Leu Ser Glu Ile

915 920 925

Ala Gln Ala Leu Val Leu Gly Phe Gly Ala Leu Gln Val Thr Arg Gly

930 935 940

Asp Ile Ser Thr Gly Val Leu Val Ala Phe Val Leu Tyr Met Gly Leu

945 950 955 960

Met Phe Gly Pro Ile Gln Gln Leu Ser Gln Ile Phe Asp Ser Tyr Gln

965 970 975

Gln Ala Ala Val Gly Phe Arg Arg Ile Thr Glu Leu Leu Ala Thr Gln

980 985 990

Pro Ser Val Pro Asp Leu Gly Thr Asn Arg His Ala Arg Gln Ala Ala

995 1000 1005

Thr Gln Pro Leu Leu Leu Asp Asp Val Thr Phe Gly Tyr Ser Asp Asp

1010 1015 1020

Pro Ile Leu Asp Asn Val Thr Val Gln Ile Glu Pro Gly Thr Thr Val

1025 1030 1035 1040

Ala Val Val Gly Pro Thr Gly Ala Gly Lys Ser Thr Val Val Lys Leu

1045 1050 1055

Leu Ser Arg Leu Tyr Asp Pro Asn Glu Gly Ala Val Lys Ala Gly Glu

1060 1065 1070

Ile Asp Ile Lys Asp Phe Pro Thr Ala Asp Trp Arg Arg Thr Ile Gly

1075 1080 1085

Thr Val Pro Gln Glu Ala His Leu Phe Ser Gly Ser Ile Ala Asp Asn

1090 1095 1100

Ile Gly Tyr Gly Cys Arg Glu Ala Ser Thr Ser Lys Ile Glu Ala Ala

1105 1110 1115 1120

Ala Arg Arg Val Gly Ala Leu Asn Ala Ile Ala Ala Ile Pro Asp Gly

1125 1130 1135

Phe Asn His Gln Val Gly Glu Arg Gly Arg Asn Leu Ser Ser Gly Gln

1140 1145 1150

Arg Gln Leu Ile Ala Leu Ala Arg Ala Glu Leu Ile Glu Pro Ser Ile

1155 1160 1165

Met Leu Leu Asp Glu Ala Thr Ser Thr Leu Asp Pro Ala Thr Glu Ala

1170 1175 1180

Val Ile Leu Asn Ala Ser Asp Arg Val Thr Lys Gly Arg Thr Ser Ile

1185 1190 1195 1200

Ile Val Ala His Arg Leu Ala Thr Ala Lys Arg Ala Asp Arg Ile Leu

1205 1210 1215

Val Val Glu Gln Gly Arg Ile Ile Glu Asp Gly Ser His Asp Ala Leu

1220 1225 1230

Leu Ser Ala Asn Gly Thr Tyr Ala Arg Met Trp His Leu Met Ala

1235 1240 1245

<210>85

<211>1081

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1051)

<223>RXA02156

<400>85

aaccactgac ctgagcttct cctacgtgga gatcaactcc gcgtacagct cttaaaaaga 60

aacagcactc caactaacaa gcagggaaaa gggcacaggc atg aat gac ttg atc 115

Met Asn Asp Leu Ile

1 5

aaa gat tta ggc tct gag gtg cgc gca aat gtc ctc gct gag gcg ttg 163

Lys Asp Leu Gly Ser Glu Val Arg Ala Asn Val Leu Ala Glu Ala Leu

10 15 20

cca tgg ttg cag cac ttc cgc gac aag att gtt gtc gtg aaa tat ggc 211

Pro Trp Leu Gln His Phe Arg Asp Lys Ile Val Val Val Lys Tyr Gly

25 30 35

gga aac gcc atg gtg gat gat gat ctc aag gct gct ttt gct gcc gac 259

Gly Asn Ala Met Val Asp Asp Asp Leu Lys Ala Ala Phe Ala Ala Asp

40 45 50

atg gtc ttc ttg cgc acc gtg ggc gca aaa cca gtg gtg gtg cac ggt 307

Met Val Phe Leu Arg Thr Val Gly Ala Lys Pro Val Val Val His Gly

55 60 65

ggt gga cct cag att tct gag atg cta aac cgt gtg ggt ctc cag ggc 355

Gly Gly Pro Gln Ile Ser Glu Met Leu Asn Arg Val Gly Leu Gln Gly

70 75 80 85

gag ttc aag ggt ggt ttc cgt gtg acc act cct gag gtc atg gac att 403

Glu Phe Lys Gly Gly Phe Arg Val Thr Thr Pro Glu Val Met Asp Ile

90 95 100

gtg cgc atg gtg ctc ttt ggt cag gtc ggt cgc gat tta gtt ggt ttg 451

Val Arg Met Val Leu Phe Gly Gln Val Gly Arg Asp Leu Val Gly Leu

105 110 115

atc aac tct cat ggc cct tac gct gtg gga acc tcc ggt gag gat gcc 499

Ile Asn Ser His Gly Pro Tyr Ala Val Gly Thr Ser Gly Glu Asp Ala

120 125 130

ggc ctg ttt acc gcg cag aag cgc atg gtc aac atc gat ggc gta ccc 547

Gly Leu Phe Thr Ala Gln Lys Arg Met Val Asn Ile Asp Gly Val Pro

135 140 145

act gat att ggt ttg gtc gga gac atc att aat gtc gat gcc tct tcc 595

Thr Asp Ile Gly Leu Val Gly Asp Ile Ile Asn Val Asp Ala Ser Ser

150 155 160 165

ttg atg gat atc atc gag gcc ggt cgc att cct gtg gtc tct acg att 643

Leu Met Asp Ile Ile Glu Ala Gly Arg Ile Pro Val Val Ser Thr Ile

170 175 180

gct cca ggc gaa gac ggc cag att tac aac att aac gcc gat acc gca 691

Ala Pro Gly Glu Asp Gly Gln Ile Tyr Asn Ile Asn Ala Asp Thr Ala

185 190 195

gca ggt gct ttg gct gca gcg att ggt gca gaa cgc ctg ctg gtt ctc 739

Ala Gly Ala Leu Ala Ala Ala Ile Gly Ala Glu Arg Leu Leu Val Leu

200 205 210

acc aat gtg gaa ggt ctg tac acc gat tgg cct gat aag agc tca ctg 787

Thr Asn Val Glu Gly Leu Tyr Thr Asp Trp Pro Asp Lys Ser Ser Leu

215 220 225

gtg tcc aag atc aag gcc acc gag ctg gag gcc att ctt ccg gga ctt 835

Val Ser Lys Ile Lys Ala Thr Glu Leu Glu Ala Ile Leu Pro Gly Leu

230 235 240 245

gat tcc ggc atg att cca aag atg gag tct tgc ttg aac gcg gtg cgt 883

Asp Ser Gly Met Ile Pro Lys Met Glu Ser Cys Leu Asn Ala Val Arg

250 255 260

ggg gga gta agc gct gct cat gtc att gac ggc cgc atc gcg cac tcg 931

Gly Gly Val Ser Ala Ala His Val Ile Asp Gly Arg Ile Ala His Ser

265 270 275

gtg ttg ctg gag ctt ttg acc atg ggt gga att ggc acg atg gtg ctg 979

Val Leu Leu Glu Leu Leu Thr Met Gly Gly Ile Gly Thr Met Val Leu

280 285 290

ccg gat gtt ttt gat cgg gag aat tat cct gaa ggc acc gtt ttt aga 1027

Pro Asp Val Phe Asp Arg Glu Asn Tyr Pro Glu Gly Thr Val Phe Arg

295 300 305

aaa gac gac aag gat ggg gaa ctg taaatgagca cgctggaaac ttggccacag 1081

Lys Asp Asp Lys Asp Gly Glu Leu

310 315

<210>86

<211>317

<212>PRT

<213>谷氨酸棒状杆菌

<400>86

Met Asn Asp Leu Ile Lys Asp Leu Gly Ser Glu Val Arg Ala Asn Val

1 5 10 15

Leu Ala Glu Ala Leu Pro Trp Leu Gln His Phe Arg Asp Lys Ile Val

20 25 30

Val Val Lys Tyr Gly Gly Asn Ala Met Val Asp Asp Asp Leu Lys Ala

35 40 45

Ala Phe Ala Ala Asp Met Val Phe Leu Arg Thr Val Gly Ala Lys Pro

50 55 60

Val Val Val His Gly Gly Gly Pro Gln Ile Ser Glu Met Leu Asn Arg

65 70 75 80

Val Gly Leu Gln Gly Glu Phe Lys Gly Gly Phe Arg Val Thr Thr Pro

85 90 95

Glu Val Met Asp Ile Val Arg Met Val Leu Phe Gly Gln Val Gly Arg

100 105 110

Asp Leu Val Gly Leu Ile Asn Ser His Gly Pro Tyr Ala Val Gly Thr

115 120 125

Ser Gly Glu Asp Ala Gly Leu Phe Thr Ala Gln Lys Arg Met Val Asn

130 135 140

Ile Asp Gly Val Pro Thr Asp Ile Gly Leu Val Gly Asp Ile Ile Asn

145 150 155 160

Val Asp Ala Ser Ser Leu Met Asp Ile Ile Glu Ala Gly Arg Ile Pro

165 170 175

Val Val Ser Thr Ile Ala Pro Gly Glu Asp Gly Gln Ile Tyr Asn Ile

180 185 190

Asn Ala Asp Thr Ala Ala Gly Ala Leu Ala Ala Ala Ile Gly Ala Glu

195 200 205

Arg Leu Leu Val Leu Thr Asn Val Glu Gly Leu Tyr Thr Asp Trp Pro

210 215 220

Asp Lys Ser Ser Leu Val Ser Lys Ile Lys Ala Thr Glu Leu Glu Ala

225 230 235 240

Ile Leu Pro Gly Leu Asp Ser Gly Met Ile Pro Lys Met Glu Ser Cys

245 250 255

Leu Asn Ala Val Arg Gly Gly Val Ser Ala Ala His Val Ile Asp Gly

260 265 270

Arg Ile Ala His Ser Val Leu Leu Glu Leu Leu Thr Met Gly Gly Ile

275 280 285

Gly Thr Met Val Leu Pro Asp Val Phe Asp Arg Glu Asn Tyr Pro Glu

290 295 300

Gly Thr Val Phe Arg Lys Asp Asp Lys Asp Gly Glu Leu

305 310 315

<210>87

<211>1561

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1531)

<223>RXA02162

<400>87

gatcgctaac aagcgcgatc gcgaagctgg caacaactaa gccacctttt caagcatcca 60

gactagaact tcaagtattt agaaagtaga agaacaccac atg gaa cag cac gga 115

Met Glu Gln His Gly

1 5

acc aat gaa ggt gcg ctg tgg ggc ggc cgc ttc tcc ggt gga ccc tcc 163

Thr Asn Glu Gly Ala Leu Trp Gly Gly Arg Phe Ser Gly Gly Pro Ser

10 15 20

gag gcc atg ttc gcc ttg agt gtc tcc act cat ttc gac tgg gtt ttg 211

Glu Ala Met Phe Ala Leu Ser Val Ser Thr His Phe Asp Trp Val Leu

25 30 35

gcc cct tat gat gtg ttg gcc tcc aag gca cac gcc aag gtt ttg cac 259

Ala Pro Tyr Asp Val Leu Ala Ser Lys Ala His Ala Lys Val Leu His

40 45 50

caa gca gat cta ctt tct gat gaa gat cta gcc acc atg ctg gct ggg 307

Gln Ala Asp Leu Leu Ser Asp Glu Asp Leu Ala Thr Met Leu Ala Gly

55 60 65

ctt gat cag ctg ggc aag gat gtc gcc gac gga acc ttc ggt ccg ctg 355

Leu Asp Gln Leu Gly Lys Asp Val Ala Asp Gly Thr Phe Gly Pro Leu

70 75 80 85

cct tct gat gag gat gtg cac ggc gcg atg gaa cgc ggt ctg att gac 403

Pro Ser Asp Glu Asp Val His Gly Ala Met Glu Arg Gly Leu Ile Asp

90 95 100

cgc gtt ggt cct gag gtg ggc ggc cgt ctg cgc gct ggt cgt tcc cgc 451

Arg Val Gly Pro Glu Val Gly Gly Arg Leu Arg Ala Gly Arg Ser Arg

105 110 115

aac gac cag gtg gca acc ctg ttc cgc atg tgg gtc cgc gac gca gtg 499

Asn Asp Gln Val Ala Thr Leu Phe Arg Met Trp Val Arg Asp Ala Val

120 125 130

cgc gac atc gcg ctg gga aca acc gag ctt gtc gac gcc ctc agc gcc 547

Arg Asp Ile Ala Leu Gly Thr Thr Glu Leu Val Asp Ala Leu Ser Ala

135 140 145

caa gct aag gca cat gca ggc gcg atc atg cca ggc aag acc cac ttc 595

Gln Ala Lys Ala His Ala Gly Ala Ile Met Pro Gly Lys Thr His Phe

150 155 160 165

cag gca gct cag ccg gtc ctt ctg gca cac cag ctg ctg gca cac gca 643

Gln Ala Ala Gln Pro Val Leu Leu Ala His Gln Leu Leu Ala His Ala

170 175 180

cag cct ttg ctg cgc gat att gat cgt atc cgt gac ctg gac aag cgt 691

Gln Pro Leu Leu Arg Asp Ile Asp Arg Ile Arg Asp Leu Asp Lys Arg

185 190 195

ctt gcg gtg tct cct tac ggt tcc ggc gca ctt gct ggt tcc tct ttg 739

Leu Ala Val Ser Pro Tyr Gly Ser Gly Ala Leu Ala Gly Ser Ser Leu

200 205 210

aag ctc aac cct gaa gca atc gct gaa gaa ctc ggc ttt gat tcc gca 787

Lys Leu Asn Pro Glu Ala Ile Ala Glu Glu Leu Gly Phe Asp Ser Ala

215 220 225

gca gat aac tcc att gat gcc acc agc tcc cgc gat ttc gca tct gaa 835

Ala Asp Asn Ser Ile Asp Ala Thr Ser Ser Arg Asp Phe Ala Ser Glu

230 235 240 245

acc gcc ttc gtg ctg gcg cag ctt gca gtg gat atg tcc cgc ttg gct 883

Thr Ala Phe Val Leu Ala Gln Leu Ala Val Asp Met Ser Arg Leu Ala

250 255 260

gaagaa atc atc gca tgg tgc acc cca gaa ttt ggt tac atc acc ttg 93l

Glu Glu Ile Ile Ala Trp Cys Thr Pro Glu Phe Gly Tyr Ile Thr Leu

265 270 275

tct gat tcc tgg tcc aca ggc agc tca atc atg ccg cag aag aag aac 979

Ser Asp Ser Trp Ser Thr Gly Ser Ser Ile Met Pro Gln Lys Lys Asn

280 285 290

cct gac gtg gca gag ctg acc cgt ggc aag tct ggt cgc ttg atc ggt 1027

Pro Asp Val Ala Glu Leu Thr Arg Gly Lys Ser Gly Arg Leu Ile Gly

295 300 305

aac ctc acc ggt ctg ctg gct acc ctg aag gca cag cct tta gcg tac 1075

Asn Leu Thr Gly Leu Leu Ala Thr Leu Lys Ala Gln Pro Leu Ala Tyr

310 315 320 325

aac cgc gac ctg cag gaa gat aag gaa cca atc gta gat tcc gtg gcg 1123

Asn Arg Asp Leu Gln Glu Asp Lys Glu Pro Ile Val Asp Ser Val Ala

330 335 340

cag ctc aac ctg ctg ctc cct gca atg act ggt ttg gtt tcc acc ttg 1171

Gln Leu Asn Leu Leu Leu Pro Ala Met Thr Gly Leu Val Ser Thr Leu

345 350 355

acc ttc aac acc gag cgc atg cgt gaa ctt gca cca gca ggt ttc acc 1219

Thr Phe Asn Thr Glu Arg Met Arg Glu Leu Ala Pro Ala Gly Phe Thr

360 365 370

ctt gcc acc gac ttg gct gag tgg atg gtg cgc cag ggc gtt cca ttc 1267

Leu Ala Thr Asp Leu Ala Glu Trp Met Val Arg Gln Gly Val Pro Phe

375 380 385

cgt gag gca cac gaa gca tcc ggc gct tgc gtg cgg atc gcg gag tcc 1315

Arg Glu Ala His Glu Ala Ser Gly Ala Cys Val Arg Ile Ala Glu Ser

390 395 400 405

agg gga gtg gac ctt atc gat ctc act gat gaa gaa ctc agt ggc gtt 1363

Arg Gly Val Asp Leu Ile Asp Leu Thr Asp Glu Glu Leu Ser Gly Val

410 415 420

gat gca cgt ctg acc cca gag gta cgg gaa gtg ctc acc att gat ggt 1411

Asp Ala Arg Leu Thr Pro Glu Val Arg Glu Val Leu Thr Ile Asp Gly

425 430 435

gca gtg gct tcc cgt gca acg cgc ggt gga acc gcg ggc gtg cgg gtt 1459

Ala Val Ala Ser Arg Ala Thr Arg Gly Gly Thr Ala Gly Val Arg Val

440 445 450

gcg gag caa cgc gca cgt gtc gat gcc gca agt acc gct cac gcg gag 1507

Ala Glu Gln Arg Ala Arg Val Asp Ala Ala Ser Thr Ala His Ala Glu

455 460 465

tgg gca cgt gcg ggg gta cgt cga taagcattag tttatggcct gtgctgcttt 1561

Trp Ala Arg Ala Gly Val Arg Arg

470 475

<210>88

<211>477

<212>PRT

<213>谷氨酸棒状杆菌

<400>88

Met Glu Gln His Gly Thr Asn Glu Gly Ala Leu Trp Gly Gly Arg Phe

1 5 10 15

Ser Gly Gly Pro Ser Glu Ala Met Phe Ala Leu Ser Val Ser Thr His

20 25 30

Phe Asp Trp Val Leu Ala Pro Tyr Asp Val Leu Ala Ser Lys Ala His

35 40 45

Ala Lys Val Leu His Gln Ala Asp Leu Leu Ser Asp Glu Asp Leu Ala

50 55 60

Thr Met Leu Ala Gly Leu Asp Gln Leu Gly Lys Asp Val Ala Asp Gly

65 70 75 80

Thr Phe Gly Pro Leu Pro Ser Asp Glu Asp Val His Gly Ala Met Glu

85 90 95

Arg Gly Leu Ile Asp Arg Val Gly Pro Glu Val Gly Gly Arg Leu Arg

100 105 110

Ala Gly Arg Ser Arg Asn Asp Gln Val Ala Thr Leu Phe Arg Met Trp

115 120 125

Val Arg Asp Ala Val Arg Asp Ile Ala Leu Gly Thr Thr Glu Leu Val

130 135 140

Asp Ala Leu Ser Ala Gln Ala Lys Ala His Ala Gly Ala Ile Met Pro

145 150 155 160

Gly Lys Thr His Phe Gln Ala Ala Gln Pro Val Leu Leu Ala His Gln

165 170 175

Leu Leu Ala His Ala Gln Pro Leu Leu Arg Asp Ile Asp Arg Ile Arg

180 185 190

Asp Leu Asp Lys Arg Leu Ala Val Ser Pro Tyr Gly Ser Gly Ala Leu

195 200 205

Ala Gly Ser Ser Leu Lys Leu Asn Pro Glu Ala Ile Ala Glu Glu Leu

210 215 220

Gly Phe Asp Ser Ala Ala Asp Asn Ser Ile Asp Ala Thr Ser Ser Arg

225 230 235 240

Asp Phe Ala Ser Glu Thr Ala Phe Val Leu Ala Gln Leu Ala Val Asp

245 250 255

Met Ser Arg Leu Ala Glu Glu Ile Ile Ala Trp Cys Thr Pro Glu Phe

260 265 270

Gly Tyr Ile Thr Leu Ser Asp Ser Trp Ser Thr Gly Ser Ser Ile Met

275 280 285

Pro Gln Lys Lys Asn Pro Asp Val Ala Glu Leu Thr Arg Gly Lys Ser

290 295 300

Gly Arg Leu Ile Gly Asn Leu Thr Gly Leu Leu Ala Thr Leu Lys Ala

305 310 315 320

Gln Pro Leu Ala Tyr Asn Arg Asp Leu Gln Glu Asp Lys Glu Pro Ile

325 330 335

Val Asp Ser Val Ala Gln Leu Asn Leu Leu Leu Pro Ala Met Thr Gly

340 345 350

Leu Val Ser Thr Leu Thr Phe Asn Thr Glu Arg Met Arg Glu Leu Ala

355 360 365

Pro Ala Gly Phe Thr Leu Ala Thr Asp Leu Ala Glu Trp Met Val Arg

370 375 380

Gln Gly Val Pro Phe Arg Glu Ala His Glu Ala Ser Gly Ala Cys Val

385 390 395 400

Arg Ile Ala Glu Ser Arg Gly Val Asp Leu Ile Asp Leu Thr Asp Glu

405 410 415

Glu Leu Ser Gly Val Asp Ala Arg Leu Thr Pro Glu Val Arg Glu Val

420 425 430

Leu Thr Ile Asp Gly Ala Val Ala Ser Arg Ala Thr Arg Gly Gly Thr

435 440 445

Ala Gly Val Arg Val Ala Glu Gln Arg Ala Arg Val Asp Ala Ala Ser

450 455 460

Thr Ala His Ala Glu Trp Ala Arg Ala Gly Val Arg Arg

465 470 475

<210>89

<211>1270

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1240)

<223>RXA02176

<400>89

gtgaagaatc tagggtgcta ggaactgaca gcttcagggt tatagttgtt gggtcagatc 60

gttaacgatc cctggccctt ttacttccaa gcgcagaaag ttg ccc gaa gac atg 115

Leu Pro Glu Asp Met

1 5

acc gac ttc ccc acc ctg ccc tct gag ttc atc cct ggc gac ggc cgt 163

Thr Asp Phe Pro Thr Leu Pro Ser Glu Phe Ile Pro Gly Asp Gly Arg

10 15 20

ttc ggc tgc gga cct tcc aag gtt cga cca gaa cag att cag gct att 211

Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu Gln Ile Gln Ala Ile

25 30 35

gtc gac gga tcc gca tcc gtc atc ggt acc tca cac cgt cag ccg gca 259

Val Asp Gly Ser Ala Ser Val Ile Gly Thr Ser His Arg Gln Pro Ala

40 45 50

gta aaa aac gtc gtg ggt tca atc cgc gag gga ctc tcc gac ctc ttc 307

Val Lys Asn Val Val Gly Ser Ile Arg Glu Gly Leu Ser Asp Leu Phe

55 60 65

tcc ctt cca gaa ggc tac gag atc atc ctt tcc cta ggt ggt gcg acc 355

Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser Leu Gly Gly Ala Thr

70 75 80 85

gca ttc tgg gat gca gca acc ttc gga ctc att gaa aag aag tcc ggt 403

Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile Glu Lys Lys Ser Gly

90 95 100

cac ctt tct ttc ggt gag ttc tcc tcc aag ttc gca aag gct tct aag 451

His Leu Ser Phe Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser Lys

105 110 115

ctt gct cct tgg ctc gac gag cca gag atc gtc acc gca gaa acc ggt 499

Leu Ala Pro Trp Leu Asp Glu Pro Glu Ile Val Thr Ala Glu Thr Gly

120 125 130

gac tct ccg gcc cca cag gca ttc gaa ggc gcc gat gtt att gca tgg 547

Asp Ser Pro Ala Pro Gln Ala Phe Glu Gly Ala Asp Val Ile Ala Trp

135 140 145

gca cac aac gaa acc tcc act ggc gcc atg gtt cca gtt ctt cgc ccc 595

Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Val Leu Arg Pro

150 155 160 165

gaa ggc tct gaa ggc tcc ctg gtt gcc att gac gca acc tcc ggc gct 643

Glu Gly Ser Glu Gly Ser Leu Val Ala Ile Asp Ala Thr Ser Gly Ala

170 175 180

ggt gga ctg cca gta gac atc aag aac tcc gat gtt tac tac ttc tcc 691

Gly Gly Leu Pro Val Asp Ile Lys Asn Ser Asp Val Tyr Tyr Phe Ser

185 190 195

cca cag aag tgc ttc gca tcc gac ggt ggc ctg tgg ctt gca gcg atg 739

Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala Met

200 205 210

agc cca gca gct ctc gag cgc atc gag aag atc aac gct tcc gat cgc 787

Ser Pro Ala Ala Leu Glu Arg Ile Glu Lys Ile Asn Ala Ser Asp Arg

215 220 225

ttc atc cct gag ttc ctc aac ctg cag acc gca gtg gat aac tcc ctg 835

Phe Ile Pro Glu Phe Leu Asn Leu Gln Thr Ala Val Asp Asn Ser Leu

230 235 240 245

aag aac cag acc tac aac acc cca gct gtt gct acc ttg ctg atg ctg 883

Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Ala Thr Leu Leu Met Leu

250 255 260

gac aac cag gtc aag tgg atg aac tcc aac ggc ggc ctg gat gga atg 931

Asp Asn Gln Val Lys Trp Met Asn Ser Asn Gly Gly Leu Asp Gly Met

265 270 275

gtt gct cgc acc aca gca agc tcc tcc gcc ctg tac aac tgg gct gag 979

Val Ala Arg Thr Thr Ala Ser Ser Ser Ala Leu Tyr Asn Trp Ala Glu

280 285 290

gct cgc gag gag gca tcc cca tac gtg gca gat gca gct aag cgc tcc 1027

Ala Arg Glu Glu Ala Ser Pro Tyr Val Ala Asp Ala Ala Lys Arg Ser

295 300 305

ctc gtt gtc ggc acc atc gac ttc gat gac tcc atc gac gca gca gtg 1075

Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Ile Asp Ala Ala Val

310 315 320 325

atc gct aag ata ctg cgc gca aac ggc atc ctg gac acc gag cct tac 1123

Ile Ala Lys Ile Leu Arg Ala Asn Gly Ile Leu Asp Thr Glu Pro Tyr

330 335 340

cgc aag ctg gga cgc aac cag ctg cgc atc ggt atg ttc cca gcg atc 1171

Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly Met Phe Pro Ala Ile

345 350 355

gat tcc acc gat gtg gaa aag ctc acc gga gca atc gac ttc atc ctc 1219

Asp Ser Thr Asp Val Glu Lys Leu Thr Gly Ala Ile Asp Phe Ile Leu

360 365 370

gat ggc ggt ttt gca agg aag taataccccc actttgaaaa acaccccgta 1270

Asp Gly Gly Phe Ala Arg Lys

375 380

<210>90

<211>380

<212>PRT

<213>谷氨酸棒状杆菌

<400>90

Leu Pro Glu Asp Met Thr Asp Phe Pro Thr Leu Pro Ser Glu Phe Ile

1 5 10 15

Pro Gly Asp Gly Arg Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu

20 25 30

Gln Ile Gln Ala Ile Val Asp Gly Ser Ala Ser Val Ile Gly Thr Ser

35 40 45

His Arg Gln Pro Ala Val Lys Asn Val Val Gly Ser Ile Arg Glu Gly

50 55 60

Leu Ser Asp Leu Phe Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser

65 70 75 80

Leu Gly Gly Ala Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile

85 90 95

Glu Lys Lys Ser Gly His Leu Ser Phe Gly Glu Phe Ser Ser Lys Phe

100 105 110

Ala Lys Ala Ser Lys Leu Ala Pro Trp Leu Asp Glu Pro Glu Ile Val

115 120 125

Thr Ala Glu Thr Gly Asp Ser Pro Ala Pro Gln Ala Phe Glu Gly Ala

130 135 140

Asp Val Ile Ala Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val

145 150 155 160

Pro Val Leu Arg Pro Glu Gly Ser Glu Gly Ser Leu Val Ala Ile Asp

165 170 175

Ala Thr Ser Gly Ala Gly Gly Leu Pro Val Asp Ile Lys Asn Ser Asp

180 185 190

Val Tyr Tyr Phe Ser Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu

195 200 205

Trp Leu Ala Ala Met Ser Pro Ala Ala Leu Glu Arg Ile Glu Lys Ile

210 215 220

Asn Ala Ser Asp Arg Phe Ile Pro Glu Phe Leu Asn Leu Gln Thr Ala

225 230 235 240

Val Asp Asn Ser Leu Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Ala

245 250 255

Thr Leu Leu Met Leu Asp Asn Gln Val Lys Trp Met Asn Ser Asn Gly

260 265 270

Gly Leu Asp Gly Met Val Ala Arg Thr Thr Ala Ser Ser Ser Ala Leu

275 280 285

Tyr Asn Trp Ala Glu Ala Arg Glu Glu Ala Ser Pro Tyr Val Ala Asp

290 295 300

Ala Ala Lys Arg Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser

305 310 315 320

Ile Asp Ala Ala Val Ile Ala Lys Ile Leu Arg Ala Asn Gly Ile Leu

325 330 335

Asp Thr Glu Pro Tyr Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly

340 345 350

Met Phe Pro Ala Ile Asp Ser Thr Asp Val Glu Lys Leu Thr Gly Ala

355 360 365

Ile Asp Phe Ile Leu Asp Gly Gly Phe Ala Arg Lys

370 375 380

<210>91

<211>973

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(943)

<223>RXA02194

<400>91

gaaatctccc agctcattta ttggacccag gtcatcatgg ttgctcgcgg cctgaagcca 60

gaagatatct acaagaacct gtaggagttt taaagcaatc atg ttg aaa atc gct 115

Met Leu Lys Ile Ala

1 5

gtc cca aac aaa ggc tcg ctg tcc gag cgc gcc atg gaa atc ctc gcc 163

Val Pro Asn Lys Gly Ser Leu Ser Glu Arg Ala Met Glu Ile Leu Ala

10 15 20

gaa gca ggc tac gca ggc cgt gga gat tcc aaa tcc ctc aac gtt ttt 211

Glu Ala Gly Tyr Ala Gly Arg Gly Asp Ser Lys Ser Leu Asn Val Phe

25 30 35

gat gaa gca aac aac gtt gaa ttc ttc ttc ctt cgc cct aaa gat atc 259

Asp Glu Ala Asn Asn Val Glu Phe Phe Phe Leu Arg Pro Lys Asp Ile

40 45 50

gcc atc tac gtt gct ggt ggc cag ctc gat ttg ggt atc acc ggc cgc 307

Ala Ile Tyr Val Ala Gly Gly Gln Leu Asp Leu Gly Ile Thr Gly Arg

55 60 65

gac ctt gct cgc gat tcc cag gct gat gtc cac gaa gtt ctt tcc ctc 355

Asp Leu Ala Arg Asp Ser Gln Ala Asp Val His Glu Val Leu Ser Leu

70 75 80 85

ggc ttc ggt tcc tcc act ttc cgt tac gca gca cca gct gat gaa gag 403

Gly Phe Gly Ser Ser Thr Phe Arg Tyr Ala Ala Pro Ala Asp Glu Glu

90 95 100

tgg agc atc gaa aag ctc gac ggc aag cgc atc gct acc tct tac ccc 451

Trp Ser Ile Glu Lys Leu Asp Gly Lys Arg Ile Ala Thr Ser Tyr Pro

105 110 115

aac ctt gtt cgc gat gac ctc gca gca cgt ggg ctt tcc gct gag gtg 499

Asn Leu Val Arg Asp Asp Leu Ala Ala Arg Gly Leu Ser Ala Glu Val

120 125 130

ctc cgc ctc gac ggt gca gta gag gta tcc atc aag ctt ggt gtc gca 547

Leu Arg Leu Asp Gly Ala Val Glu Val Ser Ile Lys Leu Gly Val Ala

135 140 145

gat gcc atc gcc gat gtt gta tcc acc ggc cgc acg ctg cgt cag caa 595

Asp Ala Ile Ala Asp Val Val Ser Thr Gly Arg Thr Leu Arg Gln Gln

150 155 160 165

ggt ctt gca cct ttc ggc gag gtt ctg tgc acc tct gag gct gtc att 643

Gly Leu Ala Pro Phe Gly Glu Val Leu Cys Thr Ser Glu Ala Val Ile

170 175 180

gtt ggc cgc aag gat gaa aag gtc acc cca gag cag cag atc ctg ctt 691

Val Gly Arg Lys Asp Glu Lys Val Thr Pro Glu Gln Gln Ile Leu Leu

185 190 195

cgc cgc atc cag gga att ttg cac gcg cag aac ttc ctc atg ctg gat 739

Arg Arg Ile Gln Gly Ile Leu His Ala Gln Asn Phe Leu Met Leu Asp

200 205 210

tac aac gtc gac cgc gac aac ctg gac gct gcc act gca gta acc cca 787

Tyr Asn Val Asp Arg Asp Asn Leu Asp Ala Ala Thr Ala Val Thr Pro

215 220 225

ggc tta tcc ggc cca acg gta tcc cca ctg gca cgc gac aac tgg gtt 835

Gly Leu Ser Gly Pro Thr Val Ser Pro Leu Ala Arg Asp Asn Trp Val

230 235 240 245

gct gta cgc gcc atg gtg cca cgc agg tca gct aac gcc atc atg gat 883

Ala Val Arg Ala Met Val Pro Arg Arg Ser Ala Asn Ala Ile Met Asp

250 255 260

aag ctt gct gga ctc ggc gct gaa gcc atc ctg gct tct gaa atc cgc 931

Lys Leu Ala Gly Leu Gly Ala Glu Ala Ile Leu Ala Ser Glu Ile Arg

265 270 275

atcgcc cgc atc tagttt taac tacccccgaa aatgtagtgg 973

Ile Ala Arg Ile

280

<210>92

<211>281

<212>PRT

<213>谷氨酸棒状杆菌

<400>92

Met Leu Lys Ile Ala Val Pro Asn Lys Gly Ser Leu Ser Glu Arg Ala

1 5 10 15

Met Glu Ile Leu Ala Glu Ala Gly Tyr Ala Gly Arg Gly Asp Ser Lys

20 25 30

Ser Leu Asn Val Phe Asp Glu Ala Asn Asn Val Glu Phe Phe Phe Leu

35 40 45

Arg Pro Lys Asp Ile Ala Ile Tyr Val Ala Gly Gly Gln Leu Asp Leu

50 55 60

Gly Ile Thr Gly Arg Asp Leu Ala Arg Asp Ser Gln Ala Asp Val His

65 70 75 80

Glu Val Leu Ser Leu Gly Phe Gly Ser Ser Thr Phe Arg Tyr Ala Ala

85 90 95

Pro Ala Asp Glu Glu Trp Ser Ile Glu Lys Leu Asp Gly Lys Arg Ile

100 105 110

Ala Thr Ser Tyr Pro Asn Leu Val Arg Asp Asp Leu Ala Ala Arg Gly

115 120 125

Leu Ser Ala Glu Val Leu Arg Leu Asp Gly Ala Val Glu Val Ser Ile

130 135 140

Lys Leu Gly Val Ala Asp Ala Ile Ala Asp Val Val Ser Thr Gly Arg

145 150 155 160

Thr Leu Arg Gln Gln Gly Leu Ala Pro Phe Gly Glu Val Leu Cys Thr

165 170 175

Ser Glu Ala Val Ile Val Gly Arg Lys Asp Glu Lys Val Thr Pro Glu

180 185 190

Gln Gln Ile Leu Leu Arg Arg Ile Gln Gly Ile Leu His Ala Gln Asn

195 200 205

Phe Leu Met Leu Asp Tyr Asn Val Asp Arg Asp Asn Leu Asp Ala Ala

210 215 220

Thr Ala Val Thr Pro Gly Leu Ser Gly Pro Thr Val Ser Pro Leu Ala

225 230 235 240

Arg Asp Asn Trp Val Ala Val Arg Ala Met Val Pro Arg Arg Ser Ala

245 250 255

Asn Ala Ile Met Asp Lys Leu Ala Gly Leu Gly Ala Glu Ala Ile Leu

260 265 270

Ala Ser Glu Ile Arg Ile Ala Arg Ile

275 280

<210>93

<211>1198

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1168)

<223>RXA02206

<400>93

gccggcatcg gcaccccaga acagctcaga gatgattcgg aggtgtccgc tggagctgat 60

cggcaggaac tctgtgaggc cttgaacgat ggagagcaca atg gtt tgt acc cag 115

Met Val Cys Thr Gln

l 5

cca ata ttt tca gtt gcg gca gga tct gct gct gcg gct agg agg gtt 163

Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala Ala Ala Arg Arg Val

10 15 20

atc tct tca ttc acc cga tct acc gta cta cct tat gac ctc agt agt 211

Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro Tyr Asp Leu Ser Ser

25 30 35

gtg gtg ggc gtg aaa cag cga atg gtc ggt tca agt ggt ttg cgg gta 259

Val Val Gly Val Lys Gln Arg Met Val Gly Ser Ser Gly Leu Arg Val

40 45 50

tcc agg ctc ggt ttg ggc acc tca aca tgg ggc tcg ggc acc gag ctg 307

Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly Ser Gly Thr Glu Leu

55 60 65

gct gag gca ggc gat atc ttt aag gcg ttc atc aat tct ggt ggc acg 355

Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile Asn Ser Gly Gly Thr

70 75 80 85

ctt atc gac gtc tcc ccc aac tac acc acc ggc gtc gcg gaa gaa atg 403

Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly Val Ala Glu Glu Met

90 95 100

ctc ggc acg atg ttg gat gcg gaa gtc tct cgt tcg gct gtc gtc att 451

Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg Ser Ala Val Val Ile

105 110 115

tcc tcc agc gca ggt gtc aac ccc gct ctg ccg ctc ggc cga cgt gtg 499

Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro Leu Gly Arg Arg Val

120 125 130

gat tgc tcc cgc cgc aat ttg att gcc caa tta gat gtc acc ctg cgg 547

Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu Asp Val Thr Leu Arg

135 140 145

gca tta aac act gac tat ttg gat ttg tgg tct gtg ggc tat tgg gat 595

Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser Val Gly Tyr Trp Asp

150 155 160 165

gag ggc acc cca ccg cat gag gtg gcc gat act ttg gat tac gcc gtg 643

Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr Leu Asp Tyr Ala Val

170 175 180

cgc acc ggc cga gtc cga tat gcc ggt gtc cga gga tat tcc ggt tgg 691

Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg Gly Tyr Ser Gly Trp

185 190 195

cag tta gcg gtc acc cac gct gca tcc aat cat gca gcg gcc tcc gcc 739

Gln Leu Ala Val Thr His Ala Ala Ser Asn His Ala Ala Ala Ser Ala

200 205 210

cgc ccc gtg gtc gtt gca caa aat gaa tac agc ctg ctg gaa cgc cgc 787

Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser Leu Leu Glu Arg Arg

215 220 225

gca gaa caa gaa ctc ctc cct gcc acc caa cac cta ggt gtc gga ttc 835

Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His Leu Gly Val Gly Phe

230 235 240 245

ttt gct ggc gct ccg ctg ggg caa ggc gtg ctg act gct aaa tac cgc 883

Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu Thr Ala Lys Tyr Arg

250 255 260

tcc gaa att ccc cat gat tcc aga gct gca tcc aca gga cgc gac gca 931

Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser Thr Gly Arg Asp Ala

265 270 275

gaa gtc caa agc tac cta gat aat cga ggc cgc atc att gtc gat gct 979

Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg Ile Ile Val Asp Ala

280 285 290

ctt gat act gca gcc aaa gga tta ggc att agc ccc gct gtc aca gcc 1027

Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser Pro Ala Val Thr Ala

295 300 305

acc acc tgg gtg cgt gat cgt ccc gga gtg aca gct gtc atc gtg ggc 1075

Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr Ala Val Ile Val Gly

310 315 320 325

gct cgc aca cat gaa cag ctg tca cat ctt ctc aag gcg gaa tcg gtg 1123

Ala Arg Thr His Glu Gln Leu Ser His Leu Leu Lys Ala Glu Ser Val

330 335 340

act ttg cca aca cca atc aca caa gcc ctt gat gat gtc tcc ctg 1168

Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp Asp Val Ser Leu

345 350 355

tgacttggtc caattacatt cactggtaat 1198

<210>94

<211>356

<212>PRT

<213>谷氨酸棒状杆菌

<400>94

Met Val Cys Thr Gln Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala

1 5 10 15

Ala Ala Arg Arg Val Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro

20 25 30

Tyr Asp Leu Ser Ser Val Val Gly Val Lys Gln Arg Met Val Gly Ser

35 40 45

Ser Gly Leu Arg Val Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly

50 55 60

Ser Gly Thr Glu Leu Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile

65 70 75 80

Asn Ser Gly Gly Thr Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly

85 90 95

Val Ala Glu Glu Met Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg

100 105 110

Ser Ala Val Val Ile Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro

115 120 125

Leu Gly Arg Arg Val Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu

130 135 140

Asp Val Thr Leu Arg Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser

145 150 155 160

Val Gly Tyr Trp Asp Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr

165 170 175

Leu Asp Tyr Ala Val Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg

180 185 190

Gly Tyr Ser Gly Trp Gln Leu Ala Val Thr His Ala Ala Ser Asn His

195 200 205

Ala Ala Ala Ser Ala Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser

210 215 220

Leu Leu Glu Arg Arg Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His

225 230 235 240

Leu Gly Val Gly Phe Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu

245 250 255

Thr Ala Lys Tyr Arg Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser

260 265 270

Thr Gly Arg Asp Ala Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg

275 280 285

Ile Ile Val Asp Ala Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser

290 295 300

Pro Ala Val Thr Ala Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr

305 310 315 320

Ala Val Ile Val Gly Ala Arg Thr His Glu Gln Leu Ser His Leu Leu

325 330 335

Lys Ala Glu Ser Val Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp

340 345 350

Asp Val Ser Leu

355

<210>95

<211>700

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(670)

<223>RXA02237

<400>95

gacgagctgg gcattgctca gacccgtcgt cttcgtggac tgggtgaccg tcagcgtcgc 60

gcacttctcg agcgtttcgg cttcgaggat taattcttca gtg tcg ggc gat aac 115

Val Ser Gly Asp Asn

1 5

caa cta gga cgg ctc gta att ctt gcg ggc ccc tca gcg gtc ggt aaa 163

Gln Leu Gly Arg Leu Val Ile Leu Ala Gly Pro Ser Ala Val Gly Lys

10 15 20

tcg act gtg gtt gat cgc ctc cgc aat gac gtt cca aac ctg tat ttc 211

Ser Thr Val Val Asp Arg Leu Arg Asn Asp Val Pro Asn Leu Tyr Phe

25 30 35

agt gtg tcg atg acc act agg gca cct cgt cct ggt gaa gtc gat gga 259

Ser Val Ser Met Thr Thr Arg Ala Pro Arg Pro Gly Glu Val Asp Gly

40 45 50

cgt gac tac ttc tat gtc act gca cag gaa ttt cag gac aaa atc gac 307

Arg Asp Tyr Phe Tyr Val Thr Ala Gln Glu Phe Gln Asp Lys Ile Asp

55 60 65

tgt gga gag atg ctt gaa tgg gca gat atc cac ggc ggt ttg cag cgt 355

Cys Gly Glu Met Leu Glu Trp Ala Asp Ile His Gly Gly Leu Gln Arg

70 75 80 85

tca ggc act cca gca ggt ccc gtc aat gag gct cgc caa aat ggt cgg 403

Ser Gly Thr Pro Ala Gly Pro Val Asn Glu Ala Arg Gln Asn Gly Arg

90 95 100

cca gta ttg gtt gag gtt gat ctt gca gga gcc cga aac atc gct agc 451

Pro Val Leu Val Glu Val Asp Leu Ala Gly Ala Arg Asn Ile Ala Ser

105 110 115

tta att cca gat gca gaa acc atc ttc ctt gct cca cct tca tgg gaa 499

Leu Ile Pro Asp Ala Glu Thr Ile Phe Leu Ala Pro Pro Ser Trp Glu

120 125 130

gtt ttg gtt gaa cgc ctc act gga cgt ggc acc gaa agc gaa gac gtt 547

Val Leu Val Glu Arg Leu Thr Gly Arg Gly Thr Glu Ser Glu Asp Val

135 140 145

att gct cgc agg ctc gag acc gca cgc gaa gaa ttg gct gct cag agc 595

Ile Ala Arg Arg Leu Glu Thr Ala Arg Glu Glu Leu Ala Ala Gln Ser

150 155 160 165

gaa ttt aag cac gtc att atc aat gat gat gtg gat aca gcc gtc aag 643

Glu Phe Lys His Val Ile Ile Asn Asp Asp Val Asp Thr Ala Val Lys

170 175 180

gct att gag gat gtt ctc ctc ggc gct tagccaaaac atagagcggt 690

Ala Ile Glu Asp Val Leu Leu Gly Ala

185 190

agggtatgct 700

<210>96

<211>190

<212>PRT

<213>谷氨酸棒状杆菌

<400>96

Val Ser Gly Asp Asn Gln Leu Gly Arg Leu Val Ile Leu Ala Gly Pro

1 5 10 15

Ser Ala Val Gly Lys Ser Thr Val Val Asp Arg Leu Arg Asn Asp Val

20 25 30

Pro Asn Leu Tyr Phe Ser Val Ser Met Thr Thr Arg Ala Pro Arg Pro

35 40 45

Gly Glu Val Asp Gly Arg Asp Tyr Phe Tyr Val Thr Ala Gln Glu Phe

50 55 60

Gln Asp Lys Ile Asp Cys Gly Glu Met Leu Glu Trp Ala Asp Ile His

65 70 75 80

Gly Gly Leu Gln Arg Ser Gly Thr Pro Ala Gly Pro Val Asn Glu Ala

85 90 95

Arg Gln Asn Gly Arg Pro Val Leu Val Glu Val Asp Leu Ala Gly Ala

100 105 110

Arg Asn Ile Ala Ser Leu Ile Pro Asp Ala Glu Thr Ile Phe Leu Ala

115 120 125

Pro Pro Ser Trp Glu Val Leu Val Glu Arg Leu Thr Gly Arg Gly Thr

130 135 140

Glu Ser Glu Asp Val Ile Ala Arg Arg Leu Glu Thr Ala Arg Glu Glu

145 150 155 160

Leu Ala Ala Gln Ser Glu Phe Lys His Val Ile Ile Asn Asp Asp Val

165 170 175

Asp Thr Ala Val Lys Ala Ile Glu Asp Val Leu Leu Gly Ala

180 185 190

<210>97

<211>1396

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1366)

<223>RXA02239

<400>97

ctatgcacag cggaggtcga gggagagcgt ggggtggcgt cgaaaagcat cttgtagagt 60

gtgtgggaac ccagccagcc tcttactttg aaggattgtt gtg ccc atg act cat 115

Val Pro Met Thr His

1 5

gtt tca agc ccc tcc gca ccc cgc aac gtg gtt gtt ggt gtt gcc ggc 163

Val Ser Ser Pro Ser Ala Pro Arg Asn Val Val Val Gly Val Ala Gly

10 15 20

gga atc gca gcg tac aag gcg tgt cac atc gtg cgc gcg ttt aaa gaa 211

Gly Ile Ala Ala Tyr Lys Ala Cys His Ile Val Arg Ala Phe Lys Glu

25 30 35

gcg ggc gat aat gtg cgg gtg gtt cct acg gaa tcc gcg ttg aag ttt 259

Ala Gly Asp Asn Val Arg Val Val Pro Thr Glu Ser Ala Leu Lys Phe

40 45 50

gtg ggg aag gcg acg ttt gaa gcg ttg tct ggc aat ccg gtg tct aca 307

Val Gly Lys Ala Thr Phe Glu Ala Leu Ser Gly Asn Pro Val Ser Thr

55 60 65

acg gtg ttt gat gcg gtg gat tcg gtg cag cat gtg aaa gtt ggc cag 355

Thr Val Phe Asp Ala Val Asp Ser Val Gln His Val Lys Val Gly Gln

70 75 80 85

gag gct gat ttg atc gtg att gcg ccg gcg aca gcc gat ttg atg gcg 403

Glu Ala Asp Leu Ile Val Ile Ala Pro Ala Thr Ala Asp Leu Met Ala

90 95 100

cgt gtg gtg gca ggt cgc ggt gac gat ctg ttg gcg gcg acg ctg ctg 451

Arg Val Val Ala Gly Arg Gly Asp Asp Leu Leu Ala Ala Thr Leu Leu

105 110 115

gtg gca acg tgc ccc gtg gtt att gcg ccg gcc atg cat acg gag atg 499

Val Ala Thr Cys Pro Val Val Ile Ala Pro Ala Met His Thr Glu Met

120 125 130

tgg ttt aat ccg gct acc gta gcc aat gtg gca acg ctg agg cag cgg 547

Trp Phe Asn Pro Ala Thr Val Ala Asn Val Ala Thr Leu Arg Gln Arg

135 140 145

ggg att acc gtg att gag cct gcg cat ggt cga ctc acc ggt aaa gat 595

Gly Ile Thr Val Ile Glu Pro Ala His Gly Arg Leu Thr Gly Lys Asp

150 155 160 165

aca ggc cct ggc cgg ctg ccg gat cca gag cag att gtt gat tta gcc 643

Thr Gly Pro Gly Arg Leu Pro Asp Pro Glu Gln Ile Val Asp Leu Ala

170 175 180

aat gcg gtg cac gcc ggg gcg agg ttg cct cag gat ttg gcg ggc aag 691

Asn Ala Val His Ala Gly Ala Arg Leu Pro Gln Asp Leu Ala Gly Lys

185 190 195

aaa gtg ctg atc act gct ggt ggc acg cat gag cat att gat cct gtg 739

Lys Val Leu Ile Thr Ala Gly Gly Thr His Glu His Ile Asp Pro Val

200 205 210

cgc ttt att ggc aat agt tcc tcg ggc cgt caa ggt ttt gcg ttg ggt 787

Arg Phe Ile Gly Asn Ser Ser Ser Gly Arg Gln Gly Phe Ala Leu Gly

215 220 225

gaa atc gca gca cag cga ggt gct cat gtc agc atc gtg gcg gga aat 835

Glu Ile Ala Ala Gln Arg Gly Ala His Val Ser Ile Val Ala Gly Asn

230 235 240 245

gct gcg gag ctg ccc act ccg gca ggc gca gag atc gtg ccg gtg gtg 883

Ala Ala Glu Leu Pro Thr Pro Ala Gly Ala Glu Ile Val Pro Val Val

250 255 260

tcc aca caa gac atg ttt gat gca gtc cag gaa cga gct ggc caa tct 931

Ser Thr Gln Asp Met Phe Asp Ala Val Gln Glu Arg Ala Gly Gln Ser

265 270 275

gat ttc atc gtc atg gcg gca gcg gta gct gat ttc acg ccc gca tcg 979

Asp Phe Ile Val Met Ala Ala Ala Val Ala Asp Phe Thr Pro Ala Ser

280 285 290

cag gcg aca tcg aag ttg aag aag ggc tca gat tct gat gaa gac gca 1027

Gln Ala Thr Ser Lys Leu Lys Lys Gly Ser Asp Ser Asp Glu Asp Ala

295 300 305

ttg agc acc atc tcg ttg gtg gaa aac ccg gat att ttg gct acc acg 1075

Leu Ser Thr Ile Ser Leu Val Glu Asn Pro Asp Ile Leu Ala Thr Thr

310 315 320 325

gtg aag cgt cgt gaa gca gga gag ctg gac agt aat cct gtc atc gtg 1123

Val Lys Arg Arg Glu Ala Gly Glu Leu Asp Ser Asn Pro Val Ile Val

330 335 340

ggt ttt gct gcg gaa act gga gac gag cac acc acc gcc ttg gag tat 1171

Gly Phe Ala Ala Glu Thr Gly Asp Glu His Thr Thr Ala Leu Glu Tyr

345 350 355

gcg cgc aag aaa ctg cag aag aag ggc tgc gac ctc ctc atg tgt aat 1219

Ala Arg Lys Lys Leu Gln Lys Lys Gly Cys Asp Leu Leu Met Cys Asn

360 365 370

gag gtg ggc atg ggc aaa gtg ttt ggg caa aag cac aat gag ggc tgg 1267

Glu Val Gly Met Gly Lys Val Phe Gly Gln Lys His Asn Glu Gly Trp

375 380 385

att ttg gat gct cac ggt ggg gta gtc gat gtg gag cac ggc agc aaa 1315

Ile Leu Asp Ala His Gly Gly Val Val Asp Val Glu His Gly Ser Lys

390 395 400 405

atc gag gtt gct gcg caa att tgg gac gcg gca ctg gcg tat cgc gaa 1363

Ile Glu Val Ala Ala Gln Ile Trp Asp Ala Ala Leu Ala Tyr Arg Glu

410 415 420

gtc tagaaaaatc cagctagacc actgacattg 1396

Val

<210>98

<211>422

<212>PRT

<213>谷氨酸棒状杆菌

<400>98

Val Pro Met Thr His Val Ser Ser Pro Ser Ala Pro Arg Asn Val Val

1 5 10 15

Val Gly Val Ala Gly Gly Ile Ala Ala Tyr Lys Ala Cys His Ile Val

20 25 30

Arg Ala Phe Lys Glu Ala Gly Asp Asn Val Arg Val Val Pro Thr Glu

35 40 45

Ser Ala Leu Lys Phe Val Gly Lys Ala Thr Phe Glu Ala Leu Ser Gly

50 55 60

Asn Pro Val Ser Thr Thr Val Phe Asp Ala Val Asp Ser Val Gln His

65 70 75 80

Val Lys Val Gly Gln Glu Ala Asp Leu Ile Val Ile Ala Pro Ala Thr

85 90 95

Ala Asp Leu Met Ala Arg Val Val Ala Gly Arg Gly Asp Asp Leu Leu

100 105 110

Ala Ala Thr Leu Leu Val Ala Thr Cys Pro Val Val Ile Ala Pro Ala

115 120 125

Met His Thr Glu Met Trp Phe Asn Pro Ala Thr Val Ala Asn Val Ala

130 135 140

Thr Leu Arg Gln Arg Gly Ile Thr Val Ile Glu Pro Ala His Gly Arg

145 150 155 160

Leu Thr Gly Lys Asp Thr Gly Pro Gly Arg Leu Pro Asp Pro Glu Gln

165 170 175

Ile Val Asp Leu Ala Asn Ala Val His Ala Gly Ala Arg Leu Pro Gln

180 185 190

Asp Leu Ala Gly Lys Lys Val Leu Ile Thr Ala Gly Gly Thr His Glu

195 200 205

His Ile Asp Pro Val Arg Phe Ile Gly Asn Ser Ser Ser Gly Arg Gln

210 215 220

Gly Phe Ala Leu Gly Glu Ile Ala Ala Gln Arg Gly Ala His Val Ser

225 230 235 240

Ile Val Ala Gly Asn Ala Ala Glu Leu Pro Thr Pro Ala Gly Ala Glu

245 250 255

Ile Val Pro Val Val Ser Thr Gln Asp Met Phe Asp Ala Val Gln Glu

260 265 270

Arg Ala Gly Gln Ser Asp Phe Ile Val Met Ala Ala Ala Val Ala Asp

275 280 285

Phe Thr Pro Ala Ser Gln Ala Thr Ser Lys Leu Lys Lys Gly Ser Asp

290 295 300

Ser Asp Glu Asp Ala Leu Ser Thr Ile Ser Leu Val Glu Asn Pro Asp

305 310 315 320

Ile Leu Ala Thr Thr Val Lys Arg Arg Glu Ala Gly Glu Leu Asp Ser

325 330 335

Asn Pro Val Ile Val Gly Phe Ala Ala Glu Thr Gly Asp Glu His Thr

340 345 350

Thr Ala Leu Glu Tyr Ala Arg Lys Lys Leu Gln Lys Lys Gly Cys Asp

355 360 365

Leu Leu Met Cys Asn Glu Val Gly Met Gly Lys Val Phe Gly Gln Lys

370 375 380

His Asn Glu Gly Trp Ile Leu Asp Ala His Gly Gly Val Val Asp Val

385 390 395 400

Glu His Gly Ser Lys Ile Glu Val Ala Ala Gln Ile Trp Asp Ala Ala

405 410 415

Leu Ala Tyr Arg Glu Val

420

<210>99

<211>709

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(679)

<223>RXA02250

<400>99

caactgaggg ttagactgtt ctttaaaagg tttgacgcct cggtttaatg cctagcagga 60

tgcgcgccac cgctaattag ttctaaggat ttgtgaaatc gtg aca acc aac gcc 115

Val Thr Thr Asn Ala

1 5

ccc gac gga gca acg aac aac atc aac aac gca cat tcg ggc gct gtc 163

Pro Asp Gly Ala Thr Asn Asn Ile Asn Asn Ala His Ser Gly Ala Val

10 15 20

ggg aag cca aag gta cag ctc agc gat gcg gaa att cag gaa tac acc 211

Gly Lys Pro Lys Val Gln Leu Ser Asp Ala Glu Ile Gln Glu Tyr Thr

25 30 35

gca gct ttc gct ggc acc acc aca acc aag cca tgg gag ctg gag gtg 259

Ala Ala Phe Ala Gly Thr Thr Thr Thr Lys Pro Trp Glu Leu Glu Val

40 45 50

acc aca aag ttt ctg aaa aag atc gcg tgg gta gcc gtg gtt gtc atc 307

Thr Thr Lys Phe Leu Lys Lys Ile Ala Trp Val Ala Val Val Val Ile

55 60 65

atg gcc gtt cac atc ttc atg ggt gcc gtg gtg gac gtc gat ttc acc 355

Met Ala Val His Ile Phe Met Gly Ala Val Val Asp Val Asp Phe Thr

70 75 80 85

ggt gca gcg gtc acc ttt gtt gac act ctg gca ttc cca gcg ttg ggc 403

Gly Ala Ala Val Thr Phe Val Asp Thr Leu Ala Phe Pro Ala Leu Gly

90 95 100

atc atc ttc tcc gtt ctt gtg ttc ttg gga ctg act cgc cct cgc gtg 451

Ile Ile Phe Ser Val Leu Val Phe Leu Gly Leu Thr Arg Pro Arg Val

105 110 115

cgt gcc aac gaa gac ggc gtt gag gtg cgt aac ttc atc gga act cgt 499

Arg Ala Asn Glu Asp Gly Val Glu Val Arg Asn Phe Ile Gly Thr Arg

120 125 130

ttc tac cca tgg gtt gtc atc tac ggc atg tct ttc ccc aag ggc agc 547

Phe Tyr Pro Trp Val Val Ile Tyr Gly Met Ser Phe Pro Lys Gly Ser

135 140 145

agc gtg gca cgt ttg gag ctt cca gac ttt gaa ttc gtt ccc atg tgg 595

Ser Val Ala Arg Leu Glu Leu Pro Asp Phe Glu Phe Val Pro Met Trp

150 155 160 165

gct ttc cag tcc cgc gat gga gaa gat gtg gtg cgc gcg gtt gcg acc 643

Ala Phe Gln Ser Arg Asp Gly Glu Asp Val Val Arg Ala Val Ala Thr

170 175 180

ttc cgc gac ctc gaa aac aag tac atg cca gag gac taattaagct 689

Phe Arg Asp Leu Glu Asn Lys Tyr Met Pro Glu Asp

185 190

gtggctgatc caacaactta 709

<210>100

<211>193

<212>PRT

<213>谷氨酸棒状杆菌

<400>100

Val Thr Thr Asn Ala Pro Asp Gly Ala Thr Asn Asn Ile Asn Asn Ala

1 5 10 15

His Ser Gly Ala Val Gly Lys Pro Lys Val Gln Leu Ser Asp Ala Glu

20 25 30

Ile Gln Glu Tyr Thr Ala Ala Phe Ala Gly Thr Thr Thr Thr Lys Pro

35 40 45

Trp Glu Leu Glu Val Thr Thr Lys Phe Leu Lys Lys Ile Ala Trp Val

50 55 60

Ala Val Val Val Ile Met Ala Val His Ile Phe Met Gly Ala Val Val

65 70 75 80

Asp Val Asp Phe Thr Gly Ala Ala Val Thr Phe Val Asp Thr Leu Ala

85 90 95

Phe Pro Ala Leu Gly Ile Ile Phe Ser Val Leu Val Phe Leu Gly Leu

100 105 110

Thr Arg Pro Arg Val Arg Ala Asn Glu Asp Gly Val Glu Val Arg Asn

115 120 125

Phe Ile Gly Thr Arg Phe Tyr Pro Trp Val Val Ile Tyr Gly Met Ser

130 135 140

Phe Pro Lys Gly Ser Ser Val Ala Arg Leu Glu Leu Pro Asp Phe Glu

145 150 155 160

Phe Val Pro Met Trp Ala Phe Gln Ser Arg Asp Gly Glu Asp Val Val

165 170 175

Arg Ala Val Ala Thr Phe Arg Asp Leu Glu Asn Lys Tyr Met Pro Glu

180 185 190

Asp

<210>101

<211>910

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(880)

<223>RXA02295

<400>101

ttcccatgct aatccgcgaa aatggcactt caacagccat atactttaaa caacacaaat 60

gatgcaagac tgataccggg atgtgatagg agcgcaccac atg ggg ttg gaa tta 115

Met Gly Leu Glu Leu

1 5

gca gct agc ggg tgg ggc atc ctc atc gca ggc gcc gcc gta gcc gga 163

Ala Ala Ser Gly Trp Gly Ile Leu Ile Ala Gly Ala Ala Val Ala Gly

10 15 20

tgg atc gac gca gta atc ggc ggt ggc gga ctc gtc ctc atc ccg ctg 211

Trp Ile Asp Ala Val Ile Gly Gly Gly Gly Leu Val Leu Ile Pro Leu

25 30 35

atc ctc gcg gtc atg ccg caa ctc gca cct gtg aca gcg ctg gcc tcc 259

Ile Leu Ala Val Met Pro Gln Leu Ala Pro Val Thr Ala Leu Ala Ser

40 45 50

aac aaa ctg gca gcc gtc acc ggc acg gca tcg gcg gca ttc acc ctg 307

Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser Ala Ala Phe Thr Leu

55 60 65

gtc agg cgc gtc aaa ccc gac aaa aaa ctg ctt gcg ctc tac gtt ctg 355

Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu Ala Leu Tyr Val Leu

70 75 80 85

gtg gca gct gtg tgc tcc ggt gca ggc gcc ctg gct gcg agt ctc att 403

Val Ala Ala Val Cys Ser Gly Ala Gly Ala Leu Ala Ala Ser Leu Ile

90 95 100

gac aaa caa atc atg cga ccg ctg atc atc gtg ttg atg ctg gtc gtt 451

Asp Lys Gln Ile Met Arg Pro Leu Ile Ile Val Leu Met Leu Val Val

105 110 115

ggc ctg atc gtg gtg ttc aaa cca aac ttc gga acc ggc gaa agc aaa 499

Gly Leu Ile Val Val Phe Lys Pro Asn Phe Gly Thr Gly Glu Set Lys

120 125 130

gcc ctg ccc acc gga tgg aaa cgc tgg gcc gcc atc gtt gca gtc gga 547

Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala Ile Val Ala Val Gly

135 140 145

ctc atc gca gcc tac gac ggc atc ttc gga ccc gga acc ggc atg ttc 595

Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro Gly Thr Gly Met Phe

150 155 160 165

ctc atc atg gcg ttc gcc gca ctg ctc tcc caa aat ttc ctg tcc tcc 643

Leu Ile Met Ala Phe Thr Ala Leu Leu Ser Gln Asn Phe Leu Ser Ser

170 175 180

gca gcc atg gcg aag gtc gta aac acc gca aca aac ctg ggt gcg cta 691

Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr Asn Leu Gly Ala Leu

185 190 195

att gta ttc atc atc ggc ggc cac atg tgg tgg acc cta gga ctc gtg 739

Ile Val Phe Ile Ile Gly Gly His Met Trp Trp Thr Leu Gly Leu Val

200 205 210

ctg gca gtc gcc aat gtc gca ggc gca caa ctc ggt gcc cga acg gtg 787

Leu Ala Val Ala Asn Val Ala Gly Ala Gln Leu Gly Ala Arg Thr Val

215 220 225

ctt ggt ggc ggt acc agg cta att aga tac gca cta cta acc ctg gtt 835

Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala Leu Leu Thr Leu Val

230 235 240 245

gtc gtc atg agc gtc tac ctc acc tgg caa caa atc caa gga atg 880

Val Val Met Ser Val Tyr Leu Thr Trp Gln Gln Ile Gln Gly Met

250 255 260

tagataagtc ggggcaaact ctaagaaatc 910

<210>102

<211>260

<212>PRT

<213>谷氨酸棒状杆菌

<400>102

Met Gly Leu Glu Leu Ala Ala Ser Gly Trp Gly Ile Leu Ile Ala Gly

1 5 10 15

Ala Ala Val Ala Gly Trp Ile Asp Ala Val Ile Gly Gly Gly Gly Leu

20 25 30

Val Leu Ile Pro Leu Ile Leu Ala Val Met Pro Gln Leu Ala Pro Val

35 40 45

Thr Ala Leu Ala Ser Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser

50 55 60

Ala Ala Phe Thr Leu Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu

65 70 75 80

Ala Leu Tyr Val Leu Val Ala Ala Val Cys Ser Gly Ala Gly Ala Leu

85 90 95

Ala Ala Ser Leu Ile Asp Lys Gln Ile Met Arg Pro Leu Ile Ile Val

100 105 110

Leu Met Leu Val Val Gly Leu Ile Val Val Phe Lys Pro Asn Phe Gly

115 120 125

Thr Gly Glu Ser Lys Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala

130 135 140

Ile Val Ala Val Gly Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro

145 150 155 160

Gly Thr Gly Met Phe Leu Ile Met Ala Phe Thr Ala Leu Leu Ser Gln

165 170 175

Asn Phe Leu Ser Ser Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr

180 185 190

Asn Leu Gly Ala Leu Ile Val Phe Ile Ile Gly Gly His Met Trp Trp

195 200 205

Thr Leu Gly Leu Val Leu Ala Val Ala Asn Val Ala Gly Ala Gln Leu

210 215 220

Gly Ala Arg Thr Val Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala

225 230 235 240

Leu Leu Thr Leu Val Val Val Met Ser Val Tyr Leu Thr Trp Gln Gln

245 250 255

Ile Gln Gly Met

260

<210>103

<211>538

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(508)

<223>RXA02299

<400>103

acgcgggggt tgttgccgga tcgaaatatt cctttccttg tcatctcacg ctatgatttc 60

taaaacttgc aggacaaccc ccataaggac accacaggac atg ctg cgc acc atc 115

Met Leu Arg Thr Ile

1 5

ctc gga agt aag att cac cga gcc act gtc act caa gct gat cta gat 163

Leu Gly Ser Lys Ile His Arg Ala Thr Val Thr Gln Ala Asp Leu Asp

10 15 20

tat gtt ggc tct gta acc atc gac gcc gac ctg gtt cac gcc gcc gga 211

Tyr Val Gly Ser Val Thr Ile Asp Ala Asp Leu Val His Ala Ala Gly

25 30 35

ttg atc gaa ggc gaa aaa gtt gcc atc gta gac atc acc aac ggc gct 259

Leu Ile Glu Gly Glu Lys Val Ala Ile Val Asp Ile Thr Asn Gly Ala

40 45 50

cgt ctg gaa act tat gtc att gtg ggc gac gcc gga acg ggc aat att 307

Arg Leu Glu Thr Tyr Val Ile Val Gly Asp Ala Gly Thr Gly Asn Ile

55 60 65

tgc atc aat ggt gcc gct gca cac ctt att aat cct ggc gat ctt gtg 355

Cys Ile Asn Gly Ala Ala Ala His Leu Ile Asn Pro Gly Asp Leu Val

70 75 80 85

atc atc atg agc tac ctt cag gca act gat gcg gaa gcc aag gcg tat 403

Ile Ile Met Ser Tyr Leu Gln Ala Thr Asp Ala Glu Ala Lys Ala Tyr

90 95 100

gag cca aag att gtg cac gtg gac gcc gac aac cgc atc gtt gcg ctc 451

Glu Pro Lys Ile Val His Val Asp Ala Asp Asn Arg Ile Val Ala Leu

105 110 115

ggc aac gat ctt gcg gaa gca cta cct gga tcc ggg ctt ttg acg tcg 499

Gly Asn Asp Leu Ala Glu Ala Leu Pro Gly Ser Gly Leu Leu Thr Ser

120 125 130

aga agc att tagcgtttta gctcgccaat attgctgccg 538

Arg Ser Ile

135

<210>104

<211>136

<212>PRT

<213>谷氨酸棒状杆菌

<400>104

Met Leu Arg Thr Ile Leu Gly Ser Lys Ile His Arg Ala Thr Val Thr

1 5 10 15

Gln Ala Asp Leu Asp Tyr Val Gly Ser Val Thr Ile Asp Ala Asp Leu

20 25 30

Val His Ala Ala Gly Leu Ile Glu Gly Glu Lys Val Ala Ile Val Asp

35 40 45

Ile Thr Asn Gly Ala Arg Leu Glu Thr Tyr Val Ile Val Gly Asp Ala

50 55 60

Gly Thr Gly Asn Ile Cys Ile Asn Gly Ala Ala Ala His Leu Ile Asn

65 70 75 80

Pro Gly Asp Leu Val Ile Ile Met Ser Tyr Leu Gln Ala Thr Asp Ala

85 90 95

Glu Ala Lys Ala Tyr Glu Pro Lys Ile Val His Val Asp Ala Asp Asn

100 105 110

Arg Ile Val Ala Leu Gly Asn Asp Leu Ala Glu Ala Leu Pro Gly Ser

115 120 125

Gly Leu Leu Thr Ser Arg Ser Ile

130 135

<210>105

<211>1426

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1396)

<223>RXA02382

<400>105

gagcagatgg ccaccgaggt cgatgtggtg gctggctact aggcctttta tggtgtgatc 60

cgacactgtt gcttcttaag ggtctgtata gtgggcaacc atg agt tca acg acc 115

Met Ser Ser Thr Thr

1 5

cta act gat gac caa att cgc gac aat gag cgg acc gaa gtt cta gct 163

Leu Thr Asp Asp Gln Ile Arg Asp Asn Glu Arg Thr Glu Val Leu Ala

10 15 20

aaa gca act gca gct aag aac atc gtc ccg gat att gca gtg ttg ggc 211

Lys Ala Thr Ala Ala Lys Asn Ile Val Pro Asp Ile Ala Val Leu Gly

25 30 35

acc gga ccg aag aac gca atc ctg cgt gcg gcg gca gat gaa ctc gtt 259

Thr Gly Pro Lys Asn Ala Ile Leu Arg Ala Ala Ala Asp Glu Leu Val

40 45 50

gca cgc agc gca gaa atc atc gaa gcc aac gct tcc gat atc gaa gcg 307

Ala Arg Ser Ala Glu Ile Ile Glu Ala Asn Ala Ser Asp Ile Glu Ala

55 60 65

ggt cgc gca aac ggc atg gaa gaa tcc atg att gat cgc ctt gcc ctt 355

Gly Arg Ala Asn Gly Met Glu Glu Ser Met Ile Asp Arg Leu Ala Leu

70 75 80 85

gat gaa tct cgc att gag ggc atc gct ggc ggt ttg cgc cag gtt gct 403

Asp Glu Ser Arg Ile Glu Gly Ile Ala Gly Gly Leu Arg Gln Val Ala

90 95 100

ggc ctg acc gac cca gtg ggt gaa gta ctg cgc gga cat gtc atg gaa 451

Gly Leu Thr Asp Pro Val Gly Glu Val Leu Arg Gly His Val Met Glu

105 110 115

aac ggc att cag atg aag cag gtc cgt gtg cct ttg ggc gtg atg ggc 499

Asn Gly Ile Gln Met Lys Gln Val Arg Val Pro Leu Gly Val Met Gly

120 125 130

atg gtc tat gaa gcc cgc cct aac gtc acc gtc gac gcc ttc ggc ctg 547

Met Val Tyr Glu Ala Arg Pro Asn Val Thr Val Asp Ala Phe Gly Leu

135 140 145

gca ctc aag tcc gga aac gta gct ttg ctg cgc ggt tcc tcc aca gct 595

Ala Leu Lys Ser Gly Asn Val Ala Leu Leu Arg Gly Ser Ser Thr Ala

150 155 160 165

gtg cat tcc aac acc aag ctc gtg gaa atc ctg cag gac gtc ctc gag 643

Val His Ser Asn Thr Lys Leu Val Glu Ile Leu Gln Asp Val Leu Glu

170 175 180

cgt ttc gag ctg cca cgc gaa acc gtg cag ttg ctg cct tgc caa acc 691

Arg Phe Glu Leu Pro Arg Glu Thr Val Gln Leu Leu Pro Cys Gln Thr

185 190 195

cgc gga tcc gtc caa gat ttg atc acc gca cgc ggc ctc gtt gac gtg 739

Arg Gly Ser Val Gln Asp Leu Ile Thr Ala Arg Gly Leu Val Asp Val

200 205 210

gtc atc cca cgc ggc ggc gca gga cta atc aac gca gtg gtc acc ggt 787

Val Ile Pro Arg Gly Gly Ala Gly Leu Ile Asn Ala Val Val Thr Gly

215 220 225

gcg acc gtg ccc acc att gaa acc ggc acc ggc aac tgc cac ttc tac 835

Ala Thr Val Pro Thr Ile Glu Thr Gly Thr Gly Asn Cys His Phe Tyr

230 235 240 245

atc gat gcc gaa gcc aag ctt gat cag gca atc gcc atg gtc atc aac 883

Ile Asp Ala Glu Ala Lys Leu Asp Gln Ala Ile Ala Met Val Ile Asn

250 255 260

ggc aag acc cgc cgc tgc agc gtg tgc aac gct act gaa acc gcg ctt 931

Gly Lys Thr Arg Arg Cys Ser Val Cys Asn Ala Thr Glu Thr Ala Leu

265 270 275

ctc gac gcc gcc ctc agc gac tca gac aag ctt gca gtc gtc cag gcg 979

Leu Asp Ala Ala Leu Ser Asp Ser Asp Lys Leu Ala Val Val Gln Ala

280 285 290

ctc cag gaa gca gga gtc aca att cat gga cgg gtg gcc gaa ttg gaa 1027

Leu Gln Glu Ala Gly Val Thr Ile His Gly Arg Val Ala Glu Leu Glu

295 300 305

gca ttc ggt gca acc gac gtg gtg gaa gca act gaa act gac tgg gat 1075

Ala Phe Gly Ala Thr Asp Val Val Glu Ala Thr Glu Thr Asp Trp Asp

310 315 320 325

tct gag tac ctg tcc ttc gat atc gct gtc gct gtg gtt gac ggt gtg 1123

Ser Glu Tyr Leu Ser Phe Asp Ile Ala Val Ala Val Val Asp Gly Val

330 335 340

gat gga gct ctg gca cac atc gct aag tac agc acc aag cac acc gaa 1171

Asp Gly Ala Leu Ala His Ile Ala Lys Tyr Ser Thr Lys His Thr Glu

345 350 355

gcg atc gcc acc caa aac att gaa acc gct cag cgc ttt gca gat cgc 1219

Ala Ile Ala Thr Gln Asn Ile Glu Thr Ala Gln Arg Phe Ala Asp Arg

360 365 370

gtc gat gca gca gcg gtg atg ata aac gca tcc acc gcc tac acc gat 1267

Val Asp Ala Ala Ala Val Met Ile Asn Ala Ser Thr Ala Tyr Thr Asp

375 380 385

ggg gag cag tac ggc atg ggc gcg gag atc ggc att tcc acc cag aaa 1315

Gly Glu Gln Tyr Gly Met Gly Ala Glu Ile Gly Ile Ser Thr Gln Lys

390 395 400 405

ctg cat gca cgt gga cca atg gcc ctg cca gag ctg acc tcc acc aag 1363

Leu His Ala Arg Gly Pro Met Ala Leu Pro Glu Leu Thr Ser Thr Lys

410 415 420

tgg att ctg cag ggc aca gga caa att agg cct taagtttgaa gaagtaatca 1416

Trp Ile Leu Gln Gly Thr Gly Gln Ile Arg Pro

425 430

agctcccctg 1426

<210>106

<211>432

<212>PRT

<213>谷氨酸棒状杆菌

<400>106

Met Ser Ser Thr Thr Leu Thr Asp Asp Gln Ile Arg Asp Asn Glu Arg

1 5 10 15

Thr Glu Val Leu Ala Lys Ala Thr Ala Ala Lys Asn Ile Val Pro Asp

20 25 30

Ile Ala Val Leu Gly Thr Gly Pro Lys Asn Ala Ile Leu Arg Ala Ala

35 40 45

Ala Asp Glu Leu Val Ala Arg Ser Ala Glu Ile Ile Glu Ala Asn Ala

50 55 60

Ser Asp Ile Glu Ala Gly Arg Ala Asn Gly Met Glu Glu Ser Met Ile

65 70 75 80

Asp Arg Leu Ala Leu Asp Glu Ser Arg Ile Glu Gly Ile Ala Gly Gly

85 90 95

Leu Arg Gln Val Ala Gly Leu Thr Asp Pro Val Gly Glu Val Leu Arg

100 105 110

Gly His Val Met Glu Asn Gly Ile Gln Met Lys Gln Val Arg Val Pro

115 120 125

Leu Gly Val Met Gly Met Val Tyr Glu Ala Arg Pro Asn Val Thr Val

130 135 140

Asp Ala Phe Gly Leu Ala Leu Lys Ser Gly Asn Val Ala Leu Leu Arg

145 150 155 160

Gly Ser Ser Thr Ala Val His Ser Asn Thr Lys Leu Val Glu Ile Leu

165 170 175

Gln Asp Val Leu Glu Arg Phe Glu Leu Pro Arg Glu Thr Val Gln Leu

180 185 190

Leu Pro Cys Gln Thr Arg Gly Ser Val Gln Asp Leu Ile Thr Ala Arg

195 200 205

Gly Leu Val Asp Val Val Ile Pro Arg Gly Gly Ala Gly Leu Ile Asn

210 215 220

Ala Val Val Thr Gly Ala Thr Val Pro Thr Ile Glu Thr Gly Thr Gly

225 230 235 240

Asn Cys His Phe Tyr Ile Asp Ala Glu Ala Lys Leu Asp Gln Ala Ile

245 250 255

Ala Met Val Ile Asn Gly Lys Thr Arg Arg Cys Ser Val Cys Asn Ala

260 265 270

Thr Glu Thr Ala Leu Leu Asp Ala Ala Leu Ser Asp Ser Asp Lys Leu

275 280 285

Ala Val Val Gln Ala Leu Gln Glu Ala Gly Val Thr Ile His Gly Arg

290 295 300

Val Ala Glu Leu Glu Ala Phe Gly Ala Thr Asp Val Val Glu Ala Thr

305 310 315 320

Glu Thr Asp Trp Asp Ser Glu Tyr Leu Ser Phe Asp Ile Ala Val Ala

325 330 335

Val Val Asp Gly Val Asp Gly Ala Leu Ala His Ile Ala Lys Tyr Ser

340 345 350

Thr Lys His Thr Glu Ala Ile Ala Thr Gln Asn Ile Glu Thr Ala Gln

355 360 365

Arg Phe Ala Asp Arg Val Asp Ala Ala Ala Val Met Ile Asn Ala Ser

370 375 380

Thr Ala Tyr Thr Asp Gly Glu Gln Tyr Gly Met Gly Ala Glu Ile Gly

385 390 395 400

Ile Ser Thr Gln Lys Leu His Ala Arg Gly Pro Met Ala Leu Pro Glu

405 410 415

Leu Thr Ser Thr Lys Trp Ile Leu Gln Gly Thr Gly Gln Ile Arg Pro

420 425 430

<210>107

<211>799

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(769)

<223>RXA02390

<400>107

gctggtggtg ctgacccata cgctggaact ccaactgctg ttgataccgc caagatgttt 60

ggccgcgagg atctcgtagc tcgcttcgag tcataggccg gtg gag tgg acc gct 115

Val Glu Trp Thr Ala

1 5

ttt ggc acc ctg att ctg ctc aat ttg gtg ggc agt tta tcc ccg ggg 163

Phe Gly Thr Leu Ile Leu Leu Asn Leu Val Gly Ser Leu Ser Pro Gly

10 15 20

cct gat acc ttt ttc ctc ctc cgc tta gcc acc cgc tcc aga gcg cac 211

Pro Asp Thr Phe Phe Leu Leu Arg Leu Ala Thr Arg Ser Arg Ala His

25 30 35

gcg atc gct ggc gtc gcc ggc atc gtc acc gga ctc acg gtg tgg gtg 259

Ala Ile Ala Gly Val Ala Gly Ile Val Thr Gly Leu Thr Val Trp Val

40 45 50

acg ctg acg gtc gtg gga gca gcg gcg ctg ctc acc act tat ccg tcg 307

Thr Leu Thr Val Val Gly Ala Ala Ala Leu Leu Thr Thr Tyr Pro Ser

55 60 65

att ctc gga atc atc cag ctc gtc ggc ggc acg tac cta agc ttc att 355

Ile Leu Gly Ile Ile Gln Leu Val Gly Gly Thr Tyr Leu Ser Phe Ile

70 75 80 85

ggg tac aag ttg ctg cgc tcg gcg tcg aga gag ctt atc gac gcc cgc 403

Gly Tyr Lys Leu Leu Arg Ser Ala Ser Arg Glu Leu Ile Asp Ala Arg

90 95 100

cag ttc cgt ttc aac gcc gat gcc cga cct atc ccg gat gcg gta gaa 451

Gln Phe Arg Phe Asn Ala Asp Ala Arg Pro Ile Pro Asp Ala Val Glu

105 110 115

gca ctg gga acc cgc act cag gta tat cga caa ggt ttg gcc acc aac 499

Ala Leu Gly Thr Arg Thr Gln Val Tyr Arg Gln Gly Leu Ala Thr Asn

120 125 130

ctg tca aac cct aaa gtt gtc atg tac ttc gcg gca att ctg gct ccg 547

Leu Ser Asn Pro Lys Val Val Met Tyr Phe Ala Ala Ile Leu Ala Pro

135 140 145

ttg atg cca gcg cac cca tca ccg gtg ctg gcg ttc tct atc atc gtg 595

Leu Met Pro Ala His Pro Ser Pro Val Leu Ala Phe Ser Ile Ile Val

150 155 160 165

gcg att tta gtg cag acc ttt gtt acc ttc tct gct gtg tgc ctc att 643

Ala Ile Leu Val Gln Thr Phe Val Thr Phe Ser Ala Val Cys Leu Ile

170 175 180

gtc tct acg gag cgt gtg cgc aaa gca atg ctg cgt gca ggt ccc tgg 691

Val Ser Thr Glu Arg Val Arg Lys Ala Met Leu Arg Ala Gly Pro Trp

185 190 195

ttt gac ctg ctt gct ggc gtt gtc ttc ctc gtt gtg ggt gtg act ctg 739

Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val Val Gly Val Thr Leu

200 205 210

ctg tat gaa ggc ctg acc ggt tta ctc ggg taaaggcata aaaaatggct 789

Leu Tyr Glu Gly Leu Thr Gly Leu Leu Gly

215 220

tccaatcaaa 799

<210>108

<211>223

<212>PRT

<213>谷氨酸棒状杆菌

<400>108

Val Glu Trp Thr Ala Phe Gly Thr Leu Ile Leu Leu Asn Leu Val Gly

1 5 10 15

Ser Leu Ser Pro Gly Pro Asp Thr Phe Phe Leu Leu Arg Leu Ala Thr

20 25 30

Arg Ser Arg Ala His Ala Ile Ala Gly Val Ala Gly Ile Val Thr Gly

35 40 45

Leu Thr Val Trp Val Thr Leu Thr Val Val Gly Ala Ala Ala Leu Leu

50 55 60

Thr Thr Tyr Pro Ser Ile Leu Gly Ile Ile Gln Leu Val Gly Gly Thr

65 70 75 80

Tyr Leu Ser Phe Ile Gly Tyr Lys Leu Leu Arg Ser Ala Ser Arg Glu

85 90 95

Leu Ile Asp Ala Arg Gln Phe Arg Phe Asn Ala Asp Ala Arg Pro Ile

100 105 110

Pro Asp Ala Val Glu Ala Leu Gly Thr Arg Thr Gln Val Tyr Arg Gln

115 120 125

Gly Leu Ala Thr Asn Leu Ser Asn Pro Lys Val Val Met Tyr Phe Ala

130 135 140

Ala Ile Leu Ala Pro Leu Met Pro Ala His Pro Ser Pro Val Leu Ala

145 150 155 160

Phe Ser Ile Ile Val Ala Ile Leu Val Gln Thr Phe Val Thr Phe Ser

165 170 175

Ala Val Cys Leu Ile Val Ser Thr Glu Arg Val Arg Lys Ala Met Leu

180 185 190

Arg Ala Gly Pro Trp Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val

195 200 205

Val Gly Val Thr Leu Leu Tyr Glu Gly Leu Thr Gly Leu Leu Gly

210 215 220

<210>109

<211>991

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(961)

<223>RXA02499

<400>109

tttgcgcacc tcacgtgatg gtttcagcat ggttctcgca ggaatcgtgg gcctggtcat 60

gacgttcggg ccgctggcaa tcgtcatgta atttgtcgtt ttg ggc ccc cgc tgc 115

Leu Gly Pro Arg Cys

1 5

tgc aaa aca ggg gtg gtt agt ggc agt gtg gga acc atg aca aca att 163

Cys Lys Thr Gly Val Val Ser Gly Ser Val Gly Thr Met Thr Thr Ile

10 15 20

gct gta atc ggc ggc gga caa atc ggc gag gct tta gtc tca ggt ttg 211

Ala Val Ile Gly Gly Gly Gln Ile Gly Glu Ala Leu Val Ser Gly Leu

25 30 35

atc gcg gcc aac atg aat cca caa aat att cgc gtc acc aac cgt tcg 259

Ile Ala Ala Asn Met Asn Pro Gln Asn Ile Arg Val Thr Asn Arg Ser

40 45 50

gaa gag cgc ggc caa gag ctg cgt gac cgc tac ggc atc ctc aac atg 307

Glu Glu Arg Gly Gln Glu Leu Arg Asp Arg Tyr Gly Ile Leu Asn Met

55 60 65

acg gat aat tcc caa gcc gca gac gaa gcc gac gtg gtg ttc ctg tgc 355

Thr Asp Asn Ser Gln Ala Ala Asp Glu Ala Asp Val Val Phe Leu Cys

70 75 80 85

gtg aag ccg aaa ttt atc gtc gaa gtg ctc tcc gaa atc acc ggc act 403

Val Lys Pro Lys Phe Ile Val Glu Val Leu Ser Glu Ile Thr Gly Thr

90 95 100

ttg gat aac aac tcc gca caa agt gtt gtg gtc agc atg gcc gca ggc 451

Leu Asp Asn Asn Ser Ala Gln Ser Val Val Val Ser Met Ala Ala Gly

105 110 115

atc agc atc gct gcc atg gaa gaa agc gcc tct gcg ggg ctc ccc gtc 499

Ile Ser Ile Ala Ala Met Glu Glu Ser Ala Ser Ala Gly Leu Pro Val

120 125 130

gtg cgc gtc atg ccg aac act cca atg ctc gtg ggc aag ggc atg tcg 547

Val Arg Val Met Pro Asn Thr Pro Met Leu Val Gly Lys Gly Met Ser

135 140 145

act gtc acc aaa ggc cgc tac gtt gac gcg gaa cag ttg gaa caa gtc 595

Thr Val Thr Lys Gly Arg Tyr Val Asp Ala Glu Gln Leu Glu Gln Val

150 155 160 165

aag gac ttg ttg agc acc gtt gga gac gtc ctc gaa gtc gcg gaa tca 643

Lys Asp Leu Leu Ser Thr Val Gly Asp Val Leu Glu Val Ala Glu Ser

170 175 180

gac atc gac gca gtc acc gcg atg tcc gga tcc tcc cct gca tac ctg 691

Asp Ile Asp Ala Val Thr Ala Met Ser Gly Ser Ser Pro Ala Tyr Leu

185 190 195

ttc ctt gtg acc gaa gcg ctc att gag gca gga gtt aat cta ggc ctg 739

Phe Leu Val Thr Glu Ala Leu Ile Glu Ala Gly Val Asn Leu Gly Leu

200 205 210

ccc cgc gcg acc gct aaa aag ctc gct gtg gcc tca ttc gaa ggt gct 787

Pro Arg Ala Thr Ala Lys Lys Leu Ala Val Ala Ser Phe Glu Gly Ala

215 220 225

gca acc atg atg aag gaa acc ggc aaa gaa ccc tca gaa ttg cgc gca 835

Ala Thr Met Met Lys Glu Thr Gly Lys Glu Pro Ser Glu Leu Arg Ala

230 235 240 245

ggc gtt tcc tca ccc gca ggc acc acc gtc gca gcc atc cga gaa ctc 883

Gly Val Ser Ser Pro Ala Gly Thr Thr Val Ala Ala Ile Arg Glu Leu

250 255 260

gaa gaa agc gga atc cga ggc gct ttc tac cgc gca gcc caa gct tgc 931

Glu Glu Ser Gly Ile Arg Gly Ala Phe Tyr Arg Ala Ala Gln Ala Cys

265 270 275

gcc gac cga tct gaa gaa ctc gga aag cgc tagaaaccgt tatttccccg 981

Ala Asp Arg Ser Glu Glu Leu Gly Lys Arg

280 285

ttatcagctc 991

<210>110

<211>287

<212>PRT

<213>谷氨酸棒状杆菌

<400>110

Leu Gly Pro Arg Cys Cys Lys Thr Gly Val Val Ser Gly Ser Val Gly

1 5 10 15

Thr Met Thr Thr Ile Ala Val Ile Gly Gly Gly Gln Ile Gly Glu Ala

20 25 30

Leu Val Ser Gly Leu Ile Ala Ala Asn Met Asn Pro Gln Asn Ile Arg

35 40 45

Val Thr Asn Arg Ser Glu Glu Arg Gly Gln Glu Leu Arg Asp Arg Tyr

50 55 60

Gly Ile Leu Asn Met Thr Asp Asn Ser Gln Ala Ala Asp Glu Ala Asp

65 70 75 80

Val Val Phe Leu Cys Val Lys Pro Lys Phe Ile Val Glu Val Leu Ser

85 90 95

Glu Ile Thr Gly Thr Leu Asp Asn Asn Ser Ala Gln Ser Val Val Val

100 105 110

Ser Met Ala Ala Gly Ile Ser Ile Ala Ala Met Glu Glu Ser Ala Ser

115 120 125

Ala Gly Leu Pro Val Val Arg Val Met Pro Asn Thr Pro Met Leu Val

130 135 140

Gly Lys Gly Met Ser Thr Val Thr Lys Gly Arg Tyr Val Asp Ala Glu

145 150 155 160

Gln Leu Glu Gln Val Lys Asp Leu Leu Ser Thr Val Gly Asp Val Leu

165 170 175

Glu Val Ala Glu Ser Asp Ile Asp Ala Val Thr Ala Met Ser Gly Ser

180 185 190

Ser Pro Ala Tyr Leu Phe Leu Val Thr Glu Ala Leu Ile Glu Ala Gly

195 200 205

Val Asn Leu Gly Leu Pro Arg Ala Thr Ala Lys Lys Leu Ala Val Ala

210 215 220

Ser Phe Glu Gly Ala Ala Thr Met Met Lys Glu Thr Gly Lys Glu Pro

225 230 235 240

Ser Glu Leu Arg Ala Gly Val Ser Ser Pro Ala Gly Thr Thr Val Ala

245 250 255

Ala Ile Arg Glu Leu Glu Glu Ser Gly Ile Arg Gly Ala Phe Tyr Arg

260 265 270

Ala Ala Gln Ala Cys Ala Asp Arg Ser Glu Glu Leu Gly Lys Arg

275 280 285

<210>111

<211>1441

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1411)

<223>RXA02516

<400>111

ttgatgatca cccactacaa gcgcatcctc aactacgtta agcctgactt cattcacgtt 60

ttcgcgaatg gccagattgt gaccaccggt ggcgctgagc ttg ctg aca agc tcg 115

Leu Leu Thr Ser Ser

1 5

agg ctg acg gct acg acc agt tca tca agt aac atg tcc gat ttc ctc 163

Arg Leu Thr Ala Thr Thr Ser Ser Ser Ser Asn Met Ser Asp Phe Leu

10 15 20

aat gca gat gga tcc ctc aat gtg gat aag gtg cgg gaa gaa ttc cca 211

Asn Ala Asp Gly Ser Leu Asn Val Asp Lys Val Arg Glu Glu Phe Pro

25 30 35

atc ctg aag cgc act gtt agg gat ggg aaa ccg ctt gct tac ctg gac 259

Ile Leu Lys Arg Thr Val Arg Asp Gly Lys Pro Leu Ala Tyr Leu Asp

40 45 50

tca ggt gcg aca tcg cag cga ccc gag cgg gtg tgg cgt gca gag gag 307

Ser Gly Ala Thr Ser Gln Arg Pro Glu Arg Val Trp Arg Ala Glu Glu

55 60 65

cac ttt gtg ctg cac acc aac gcc ccc gtg cac cgc ggt gcc tac caa 355

His Phe Val Leu His Thr Asn Ala Pro Val His Arg Gly Ala Tyr Gln

70 75 80 85

ctg gct gag gaa gca acg gat gct tat gaa ggt gcc cgc gag aag atc 403

Leu Ala Glu Glu Ala Thr Asp Ala Tyr Glu Gly Ala Arg Glu Lys Ile

90 95 100

gct gcc ttt gtt ggt gcc gag cag cat gaa att gcg ttc act aag aat 451

Ala Ala Phe Val Gly Ala Glu Gln His Glu Ile Ala Phe Thr Lys Asn

105 110 115

gca act gaa gca ctc aat ctt gtt gcg tac acc ttg ggt gat gac cgt 499

Ala Thr Glu Ala Leu Asn Leu Val Ala Tyr Thr Leu Gly Asp Asp Arg

120 125 130

tcc ggt aag tat cgt gtc cag gcc ggg gat acc gtg gtc atc acg gag 547

Ser Gly Lys Tyr Arg Val Gln Ala Gly Asp Thr Val Val Ile Thr Glu

135 140 145

cta gag cac cac gca aac ttg gtg cca tgg cag gag ctg tgc cgt cga 595

Leu Glu His His Ala Asn Leu Val Pro Trp Gln Glu Leu Cys Arg Arg

150 155 160 165

acc ggt gcg aca ttg aag tgg tac aag gtg act gaa gat ggt cgc att 643

Thr Gly Ala Thr Leu Lys Trp Tyr Lys Val Thr Glu Asp Gly Arg Ile

170 175 180

gat ctc gat tca ctc gag ctt gat gaa act gtc aag gtc gtt gcc ttc 691

Asp Leu Asp Ser Leu Glu Leu Asp Glu Thr Val Lys Val Val Ala Phe

185 190 195

act cac cag tcc aat gtg acc ggt gct gtg gct gat gtt cca gag ttg 739

Thr His Gln Ser Asn Val Thr Gly Ala Val Ala Asp Val Pro Glu Leu

200 205 210

gtt cgt cgt gcc aag gct gtc ggc gct ctc acg gtg ctt gat gcg tgc 787

Val Arg Arg Ala Lys Ala Val Gly Ala Leu Thr Val Leu Asp Ala Cys

215 220 225

cag tct gtt cct cat atg cca gtg aat ttc cac gag ctg gat gta gat 835

Gln Ser Val Pro His Met Pro Val Asn Phe His Glu Leu Asp Val Asp

230 235 240 245

ttc tct gca ttc tct ggc cat aag atg ctg gga cct gca ggc gtg ggc 883

Phe Ser Ala Phe Ser Gly His Lys Met Leu Gly Pro Ala Gly Val Gly

250 255 260

gtt gtg tat gca aag tcc cca atc ttg gat gaa ctg cca cca ttt ttg 931

Val Val Tyr Ala Lys Ser Pro Ile Leu Asp Glu Leu Pro Pro Phe Leu

265 270 275

act ggt ggt tcc atg att gaa gtt gtc acc atg gag ggt tcc acc tac 979

Thr Gly Gly Ser Met Ile Glu Val Val Thr Met Glu Gly Ser Thr Tyr

280 285 290

gct gcc gca cct caa cgt ttt gag gcc ggc acg cag atg acc agc cag 1027

Ala Ala Ala Pro Gln Arg Phe Glu Ala Gly Thr Gln Met Thr Ser Gln

295 300 305

gtt gtg ggc ttg ggt gct gcc gtg gac atg ctg aat gaa atc ggt atg 1075

Val Val Gly Leu Gly Ala Ala Val Asp Met Leu Asn Glu Ile Gly Met

310 315 320 325

gaa gca atc gca gcg cat gag cac gca ttg act gct tac gcg ttg gaa 1123

Glu Ala Ile Ala Ala His Glu His Ala Leu Thr Ala Tyr Ala Leu Glu

330 335 340

aag ctc acg gca att aag gga cta acc att gct ggt cct ttg act gca 1171

Lys Leu Thr Ala Ile Lys Gly Leu Thr Ile Ala Gly Pro Leu Thr Ala

345 350 355

gag cag cgc ggc ggt gca atc agc ttc ggt gtc gag ggc att cac cca 1219

Glu Gln Arg Gly Gly Ala Ile Ser Phe Gly Val Glu Gly Ile His Pro

360 365 370

cac gat cta ggc caa gtg ctt gac gat cag ggc gtg aat atc cgc gtc 1267

His Asp Leu Gly Gln Val Leu Asp Asp Gln Gly Val Asn Ile Arg Val

375 380 385

ggc cac cac tgc gcg tgg ccc gtg cac cgc agc atg aac gta caa tcg 1315

Gly His His Cys Ala Trp Pro Val His Arg Ser Met Asn Val Gln Ser

390 395 400 405

aca gca aga gca tct ttc tat ctc tat aac acc ttc gaa gaa atc gac 1363

Thr Ala Arg Ala Ser Phe Tyr Leu Tyr Asn Thr Phe Glu Glu Ile Asp

410 415 420

cgc ctc gcg gca gcg atc gag aag gcc aag caa ttc ttt gga gtt gag 1411

Arg Leu Ala Ala Ala Ile Glu Lys Ala Lys Gln Phe Phe Gly Val Glu

425 430 435

taatgaacct tgagcagatg taccaggagg 1441

<210>112

<211>437

<212>PRT

<213>谷氨酸棒状杆菌

<400>112

Leu Leu Thr Ser Ser Arg Leu Thr Ala Thr Thr Ser Ser Ser Ser Asn

1 5 10 15

Met Ser Asp Phe Leu Asn Ala Asp Gly Ser Leu Asn Val Asp Lys Val

20 25 30

Arg Glu Glu Phe Pro Ile Leu Lys Arg Thr Val Arg Asp Gly Lys Pro

35 40 45

Leu Ala Tyr Leu Asp Ser Gly Ala Thr Ser Gln Arg Pro Glu Arg Val

50 55 60

Trp Arg Ala Glu Glu His Phe Val Leu His Thr Asn Ala Pro Val His

65 70 75 80

Arg Gly Ala Tyr Gln Leu Ala Glu Glu Ala Thr Asp Ala Tyr Glu Gly

85 90 95

Ala Arg Glu Lys Ile Ala Ala Phe Val Gly Ala Glu Gln His Glu Ile

100 105 110

Ala Phe Thr Lys Asn Ala Thr Glu Ala Leu Asn Leu Val Ala Tyr Thr

115 120 125

Leu Gly Asp Asp Arg Ser Gly Lys Tyr Arg Val Gln Ala Gly Asp Thr

130 135 140

Val Val Ile Thr Glu Leu Glu His His Ala Asn Leu Val Pro Trp Gln

145 150 155 160

Glu Leu Cys Arg Arg Thr Gly Ala Thr Leu Lys Trp Tyr Lys Val Thr

165 170 175

Glu Asp Gly Arg Ile Asp Leu Asp Ser Leu Glu Leu Asp Glu Thr Val

180 185 190

Lys Val Val Ala Phe Thr His Gln Ser Asn Val Thr Gly Ala Val Ala

195 200 205

Asp Val Pro Glu Leu Val Arg Arg Ala Lys Ala Val Gly Ala Leu Thr

210 215 220

Val Leu Asp Ala Cys Gln Ser Val Pro His Met Pro Val Asn Phe His

225 230 235 240

Glu Leu Asp Val Asp Phe Ser Ala Phe Ser Gly His Lys Met Leu Gly

245 250 255

Pro Ala Gly Val Gly Val Val Tyr Ala Lys Ser Pro Ile Leu Asp Glu

260 265 270

Leu Pro Pro Phe Leu Thr Gly Gly Ser Met Ile Glu Val Val Thr Met

275 280 285

Glu Gly Ser Thr Tyr Ala Ala Ala Pro Gln Arg Phe Glu Ala Gly Thr

290 295 300

Gln Met Thr Ser Gln Val Val Gly Leu Gly Ala Ala Val Asp Met Leu

305 310 315 320

Asn Glu Ile Gly Met Glu Ala Ile Ala Ala His Glu His Ala Leu Thr

325 330 335

Ala Tyr Ala Leu Glu Lys Leu Thr Ala Ile Lys Gly Leu Thr Ile Ala

340 345 350

Gly Pro Leu Thr Ala Glu Gln Arg Gly Gly Ala Ile Ser Phe Gly Val

355 360 365

Glu Gly Ile His Pro His Asp Leu Gly Gln Val Leu Asp Asp Gln Gly

370 375 380

Val Asn Ile Arg Val Gly His His Cys Ala Trp Pro Val His Arg Ser

385 390 395 400

Met Asn Val Gln Ser Thr Ala Arg Ala Ser Phe Tyr Leu Tyr Asn Thr

405 410 415

Phe Glu Glu Ile Asp Arg Leu Ala Ala Ala Ile Glu Lys Ala Lys Gln

420 425 430

Phe Phe Gly Val Glu

435

<210>113

<211>1276

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1246)

<223>RXA02532

<400>113

agataccgac tttgctaatc ccaccaccgt agccactagg tatttagttg ccttggacaa 60

gcgtgtacct cgtgcttgat ataacaagta aggaagcctg atg aat ttt tac cca 115

Met Asn Phe Tyr Pro

1 5

cca tct gta cct att aac oct gcg tgg cgt cca ccc aca gta act gtg 163

Pro Ser Val Pro Ile Asn Pro Ala Trp Arg Pro Pro Thr Val Thr Val

10 15 20

caa gcg gga cgg cca gcc aga act cct ggt gcg ccg atg aac cca cct 211

Gln Ala Gly Arg Pro Ala Arg Thr Pro Gly Ala Pro Met Asn Pro Pro

25 30 35

atc acg ttg tcc agc act tat gtt cat gat tca gaa aaa gct tat ggg 259

Ile Thr Leu Ser Ser Thr Tyr Val His Asp Ser Glu Lys Ala Tyr Gly

40 45 50

cgc gat ggc aat gat gga tgg ggt gca ttt gag gct gcc atg gga act 307

Arg Asp Gly Asn Asp Gly Trp Gly Ala Phe Glu Ala Ala Met Gly Thr

55 60 65

cta gat ggt ggg ttc gcg gta tct tat tct tca ggt ttg gca gcg gca 355

Leu Asp Gly Gly Phe Ala Val Ser Tyr Ser Ser Gly Leu Ala Ala Ala

70 75 80 85

acg tcg att gct gat ttg gtt cct act ggt ggc aca gtt gtt tta cct 403

Thr Ser Ile Ala Asp Leu Val Pro Thr Gly Gly Thr Val Val Leu Pro

90 95 100

aaa gct gcc tat tat ggc gtg acc aat att ttc gcc agg atg gaa gcc 451

Lys Ala Ala Tyr Tyr Gly Val Thr Asn Ile Phe Ala Arg Met Glu Ala

105 110 115

cgc gga agg ctg aag gtt cga act gtt gat gca gac aat acc gaa gaa 499

Arg Gly Arg Leu Lys Val Arg Thr Val Asp Ala Asp Asn Thr Glu Glu

120 125 130

gtg att gct gct gct caa ggt gca gat gtg gtg tgg gtg gaa tcg atc 547

Val Ile Ala Ala Ala Gln Gly Ala Asp Val Val Trp Val Glu Ser Ile

135 140 145

gct aat ccg acg atg gtg gta gct gat atc cct gca ata gtc gac ggt 595

Ala Asn Pro Thr Met Val Val Ala Asp Ile Pro Ala Ile Val Asp Gly

150 155 160 165

gtg cgt ggg ctt gga gtt ttg act gtc gtt gac gcg act ttc gca acg 643

Val Arg Gly Leu Gly Val Leu Thr Val Val Asp Ala Thr Phe Ala Thr

170 175 180

cca ctt cgt caa cgt cca ttg gaa ctt ggt gct gat att gtg ctt tac 691

Pro Leu Arg Gln Arg Pro Leu Glu Leu Gly Ala Asp Ile Val Leu Tyr

185 190 195

tcg gca acc aaa ctt atc ggt gga cac tct gat ctt ctt ctt gga gtc 739

Ser Ala Thr Lys Leu Ile Gly Gly His Ser Asp Leu Leu Leu Gly Val

200 205 210

gca gtg tgc aag tct gag cac cat gcg cag ttt ctt gcc act cac cgt 787

Ala Val Cys Lys Ser Glu His His Ala Gln Phe Leu Ala Thr His Arg

215 220 225

cat gat cat ggt tca gtg ccg gga ggt ctt gaa gcg ttt ctt gct ctc 835

His Asp His Gly Ser Val Pro Gly Gly Leu Glu Ala Phe Leu Ala Leu

230 235 240 245

cgt gga ttg tat tcc ttg gcg gtg cgt ctt gat cga gca gaa tcc aac 883

Arg Gly Leu Tyr Ser Leu Ala Val Arg Leu Asp Arg Ala Glu Ser Asn

250 255 260

gca gca gaa ctt tcg cgg cga ctt aac gcg cat cct tcg gtt acc cgc 931

Ala Ala Glu Leu Ser Arg Arg Leu Asn Ala His Pro Ser Val Thr Arg

265 270 275

gtc aat tat cca gga ctt cct gat gat ccc caa cat gaa aaa gcc gtg 979

Val Asn Tyr Pro Gly Leu Pro Asp Asp Pro Gln His Glu Lys Ala Val

280 285 290

cga gtc cta ccc tct gga tgt gga aac atg ttg tca ttt gag ctt gat 1027

Arg Val Leu Pro Ser Gly Cys Gly Asn Met Leu Ser Phe Glu Leu Asp

295 300 305

gca aca cct gaa cga act gat gag att ctc gaa agc ctg tca ctt tta 1075

Ala Thr Pro Glu Arg Thr Asp Glu Ile Leu Glu Ser Leu Ser Leu Leu

310 315 320 325

acc cac gcg acc agt tgg gga ggt gtg gaa aca gcc att gaa cgt cgc 1123

Thr His Ala Thr Ser Trp Gly Gly Val Glu Thr Ala Ile Glu Arg Arg

330 335 340

acc agg cgg gat gct gaa gtg gtg gca gga gta ccg atg act ctt tgc 1171

Thr Arg Arg Asp Ala Glu Val Val Ala Gly Val Pro Met Thr Leu Cys

345 350 355

cgc gtt tcc gta gga att gaa gac gtt gaa gat cta tgg gaa gac ctc 1219

Arg Val Ser Val Gly Ile Glu Asp Val Glu Asp Leu Trp Glu Asp Leu

360 365 370

aac gcc tca atc gac aaa gtt ctg ggt tagaactcgt agccagtaac 1266

Asn Ala Ser Ile Asp Lys Val Leu Gly

375 380

cagaccttca 1276

<210>114

<211>382

<212>PRT

<213>谷氨酸棒状杆菌

<400>114

Met Asn Phe Tyr Pro Pro Ser Val Pro Ile Asn Pro Ala Trp Arg Pro

1 5 10 15

Pro Thr Val Thr Val Gln Ala Gly Arg Pro Ala Arg Thr Pro Gly Ala

20 25 30

Pro Met Asn Pro Pro Ile Thr Leu Ser Ser Thr Tyr Val His Asp Ser

35 40 45

Glu Lys Ala Tyr Gly Arg Asp Gly Asn Asp Gly Trp Gly Ala Phe Glu

50 55 60

Ala Ala Met Gly Thr Leu Asp Gly Gly Phe Ala Val Ser Tyr Ser Ser

65 70 75 80

Gly Leu Ala Ala Ala Thr Ser Ile Ala Asp Leu Val Pro Thr Gly Gly

85 90 95

Thr Val Val Leu Pro Lys Ala Ala Tyr Tyr Gly Val Thr Asn Ile Phe

100 105 110

Ala Arg Met Glu Ala Arg Gly Arg Leu Lys Val Arg Thr Val Asp Ala

115 120 125

Asp Asn Thr Glu Glu Val Ile Ala Ala Ala Gln Gly Ala Asp Val Val

130 135 140

Trp Val Glu Ser Ile Ala Asn Pro Thr Met Val Val Ala Asp Ile Pro

145 150 155 160

Ala Ile Val Asp Gly Val Arg Gly Leu Gly Val Leu Thr Val Val Asp

165 170 175

Ala Thr Phe Ala Thr Pro Leu Arg Gln Arg Pro Leu Glu Leu Gly Ala

180 185 190

Asp Ile Val Leu Tyr Ser Ala Thr Lys Leu Ile Gly Gly His Ser Asp

195 200 205

Leu Leu Leu Gly Val Ala Val Cys Lys Ser Glu His His Ala Gln Phe

210 215 220

Leu Ala Thr His Arg His Asp His Gly Ser Val Pro Gly Gly Leu Glu

225 230 235 240

Ala Phe Leu Ala Leu Arg Gly Leu Tyr Ser Leu Ala Val Arg Leu Asp

245 250 255

Arg Ala Glu Ser Asn Ala Ala Glu Leu Ser Arg Arg Leu Asn Ala His

260 265 270

Pro Ser Val Thr Arg Val Asn Tyr Pro Gly Leu Pro Asp Asp Pro Gln

275 280 285

His Glu Lys Ala Val Arg Val Leu Pro Ser Gly Cys Gly Asn Met Leu

290 295 300

Ser Phe Glu Leu Asp Ala Thr Pro Glu Arg Thr Asp Glu Ile Leu Glu

305 310 315 320

Ser Leu Ser Leu Leu Thr His Ala Thr Ser Trp Gly Gly Val Glu Thr

325 330 335

Ala Ile Glu Arg Arg Thr Arg Arg Asp Ala Glu Val Val Ala G1y Val

340 345 350

Pro Met Thr Leu Cys Arg Val Ser Val Gly Ile Glu Asp Val Glu Asp

355 360 365

Leu Trp Glu Asp Leu Asn Ala Ser Ile Asp Lys Val Leu Gly

370 375 380

<210>115

<211>928

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(898)

<223>RXA02536

<400>115

aggtacatgc tgctgattca agtggttttt acgatgctgg gcaagaagtg atcacgcgaa 60

cctgtgtata acttgcctca aagcgcctag gctgtggatt atg cgt att gcc ttg 115

Met Arg Ile Ala Leu

1 5

ctt cag atc tcg acg aat tcc gat aag atg gac aac ttc gcc ctg ctg 163

Leu Gln Ile Ser Thr Asn Ser Asp Lys Met Asp Asn Phe Ala Leu Leu

10 15 20

cgt gat gct gct gaa aaa gct gcg gaa cag ggg gct cgg gtg ttg gtg 211

Arg Asp Ala Ala Glu Lys Ala Ala Glu Gln Gly Ala Arg Val Leu Val

25 30 35

ttt ccg gag gcg act tcg caa agc ttt ggt acg gga agg ctt gat act 259

Phe Pro Glu Ala Thr Ser Gln Ser Phe Gly Thr Gly Arg Leu Asp Thr

40 45 50

cag gcg gag gag ctc gat ggc gaa ttc tcc acc gcg gta cga aaa tta 307

Gln Ala Glu Glu Leu Asp Gly Glu Phe Ser Thr Ala Val Arg Lys Leu

55 60 65

gcc gat gag ctg gac gtt gtc atc gtt gcg ggc atg ttc acc cct gct 355

Ala Asp Glu Leu Asp Val Val Ile Val Ala Gly Met Phe Thr Pro Ala

70 75 80 85

gac acc gtg cag cgc ggt gaa aaa acg atc tcg cgc gtc aac aac acc 403

Asp Thr Val Gln Arg Gly Glu Lys Thr Ile Ser Arg Val Asn Asn Thr

90 95 100

gtg ctg att agt ggc gct gga ttg cat cag gga tac aac aaa att cac 451

Val Leu Ile Ser Gly Ala Gly Leu His Gln Gly Tyr Asn Lys Ile His

105 110 115

aca tat gac gcg ttc ggt tat agg gaa tcc gac act gtg aaa ccg ggc 499

Thr Tyr Asp Ala Phe Gly Tyr Arg Glu Ser Asp Thr Val Lys Pro Gly

120 125 130

gat gag ctg gtt gta ttc gag gtc gac gat att aaa ttt ggt gtg gcg 547

Asp Glu Leu Val Val Phe Glu Val Asp Asp Ile Lys Phe Gly Val Ala

135 140 145

aca tgc tac gat att cga ttc cca gaa cag ttc aaa gac ctc gcc cgc 595

Thr Cys Tyr Asp Ile Arg Phe Pro Glu Gln Phe Lys Asp Leu Ala Arg

150 155 160 165

aac ggt gca cag ata att gtg gtt ccc acg tcg tgg caa gac ggt cct 643

Asn Gly Ala Gln Ile Ile Val Val Pro Thr Ser Trp Gln Asp Gly Pro

170 175 180

gga aaa tta gaa caa tgg gaa gtc ctc cct cgc gcg cgt gca ctg gat 691

Gly Lys Leu Glu Gln Trp Glu Val Leu Pro Arg Ala Arg Ala Leu Asp

185 190 195

tcc acc tgc tgg atc gta gcg tgt ggg caa gcg cga ctt cca gaa gaa 739

Ser Thr Cys Trp Ile Val Ala Cys Gly Gln Ala Arg Leu Pro Glu Glu

200 205 210

tta cgc gat gaa cga aaa ggc cct acg ggg att ggt cat tcc atg gtg 787

Leu Arg Asp Glu Arg Lys Gly Pro Thr Gly Ile Gly His Ser Met Val

215 220 225

aca aac cca cac ggt gaa gta att gct agc gcg ggt tat gag cca gaa 835

Thr Asn Pro His Gly Glu Val Ile Ala Ser Ala Gly Tyr Glu Pro Glu

230 235 240 245

atg ttg atc gcg gat att gat gtc agc ggt ttg gcc aaa att cgg gag 883

Met Leu Ile Ala Asp Ile Asp Val Ser Gly Leu Ala Lys Ile Arg Glu

250 255 260

gca ttg cct gtt ctt taaccactgt ctaaggaatc actcgttggg 928

Ala Leu Pro Val Leu

265

<210>116

<211>266

<212>PRT

<213>谷氨酸棒状杆菌

<400>116

Met Arg Ile Ala Leu Leu Gln Ile Ser Thr Asn Ser Asp Lys Met Asp

1 5 10 15

Asn Phe Ala Leu Leu Arg Asp Ala Ala Glu Lys Ala Ala Glu Gln Gly

20 25 30

Ala Arg Val Leu Val Phe Pro Glu Ala Thr Ser Gln Ser Phe Gly Thr

35 40 45

Gly Arg Leu Asp Thr Gln Ala Glu Glu Leu Asp Gly Glu Phe Ser Thr

50 55 60

Ala Val Arg Lys Leu Ala Asp Glu Leu Asp Val Val Ile Val Ala Gly

65 70 75 80

Met Phe Thr Pro Ala Asp Thr Val Gln Arg Gly Glu Lys Thr Ile Ser

85 90 95

Arg Val Asn Asn Thr Val Leu Ile Ser Gly Ala Gly Leu His Gln Gly

100 105 110

Tyr Asn Lys Ile His Thr Tyr Asp Ala Phe Gly Tyr Arg Glu Ser Asp

115 120 125

Thr Val Lys Pro Gly Asp Glu Leu Val Val Phe Glu Val Asp Asp Ile

130 135 140

Lys Phe Gly Val Ala Thr Cys Tyr Asp Ile Arg Phe Pro Glu Gln Phe

145 150 155 160

Lys Asp Leu Ala Arg Asn Gly Ala Gln Ile Ile Val Val Pro Thr Ser

165 170 175

Trp Gln Asp Gly Pro Gly Lys Leu Glu Gln Trp Glu Val Leu Pro Arg

180 185 190

Ala Arg Ala Leu Asp Ser Thr Cys Trp Ile Val Ala Cys Gly Gln Ala

195 200 205

Arg Leu Pro Glu Glu Leu Arg Asp Glu Arg Lys Gly Pro Thr Gly Ile

210 215 220

Gly His Ser Met Val Thr Asn Pro His Gly Glu Val Ile Ala Ser Ala

225 230 235 240

Gly Tyr Glu Pro Glu Met Leu Ile Ala Asp Ile Asp Val Ser Gly Leu

245 250 255

Ala Lys Ile Arg Glu Ala Leu Pro Val Leu

260 265

<210>117

<211>997

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(967)

<223>RXA02560

<400>117

ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc 60

ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115

Met Gln Gly Asn Ser

1 5

ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163

Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro

10 15 20

gga gaa ctt tta gcc gcc cgc tac gga caa cct gca acc tgg acg cca 211

Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro Ala Thr Trp Thr Pro

25 30 35

ccg cag tgg aat gag acg ctt gat gtc att cac cag cat cga tca gtt 259

Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His Gln His Arg Ser Val

40 45 50

cgc agg tgg ttg gat aaa ccg gtt gat gat gac acc atc cgc acc att 307

Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp Thr Ile Arg Thr Ile

55 60 65

att tcc gcc gca caa tcg gct gga acc tct tcc aat aag cag gtc att 355

Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser Asn Lys Gln Val Ile

70 75 80 85

tct gtc atc gtg gtt aaa gat cct gag ctg agg aaa ggc ctc gcg ggg 403

Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg Lys Gly Leu Ala Gly

90 95 100

atc act cgc cag atg ttt ccg cac ctt gag cag gtt ccc gcg gtg ctg 451

Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln Val Pro Ala Val Leu

105 110 115

att tgg ttg att gat tat tcc cga atc agt gcg gtg gca gcc aga gaa 499

Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala Val Ala Ala Arg Glu

120 125 130

gat ctc cca aca ggg gct ctt gat tat ctc gat gag gcc gcg ttg ggg 547

Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp Glu Ala Ala Leu Gly

135 140 145

ttc ctc gac gcc gga atc gca gct caa aac gct gca att gct gcg gag 595

Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala Ala Ile Ala Ala Glu

150 155 160 165

tca ctt gga ttg gga acg ctc tat ttg ggt tcg gtg cgc aac gat gcg 643

Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser Val Arg Asn Asp Ala

170 175 180

gaa gcc gtg cac aaa ttg ctt ggc ctt cca cct gag atc gtg cct gtc 691

Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro Glu Ile Val Pro Val

185 190 195

gtg ggc ttg gaa att ggg cat gcg gat ccg tct gaa cct gcc gga att 739

Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser Glu Pro Ala Gly Ile

200 205 210

aaa cct cgc ctg cca caa gaa gcc att gtt cac tgg gat acc tac acc 787

Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His Trp Asp Thr Tyr Thr

215 220 225

gag aaa aac ctc gaa ctt atc gat tcc tac gac cgc gcc ctc gac act 835

Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp Arg Ala Leu Asp Thr

230 235 240 245

tac tat tct cgc tac ggc cag cac cag ctc tgg tcg aag cag acg gcg 883

Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp Ser Lys Gln Thr Ala

250 255 260

cat agg gcg gcg tcg aaa agc ttt tca aaa acc aac agg cag ttc ctt 931

His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr Asn Arg Gln Phe Leu

265 270 275

agg ggc gtg ttt gag cgc gcc ggg ttt ggg ctg aga taaaagcatg 977

Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu Arg

280 285

attatggacg cctccctccc 997

<210>118

<211>289

<212>PRT

<213>谷氨酸棒状杆菌

<400>118

Met Gln Gly Asn Ser Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys

1 5 10 15

Pro Met Pro Ser Pro Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro

20 25 30

Ala Thr Trp Thr Pro Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His

35 40 45

Gln His Arg Ser Val Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp

50 55 60

Thr Ile Arg Thr Ile Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser

65 70 75 80

Asn Lys Gln Val Ile Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg

85 90 95

Lys Gly Leu Ala Gly Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln

100 105 110

Val Pro Ala Val Leu Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala

115 120 125

Val Ala Ala Arg Glu Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp

130 135 140

Glu Ala Ala Leu Gly Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala

145 150 155 160

Ala Ile Ala Ala Glu Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser

165 170 175

Val Arg Asn Asp Ala Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro

180 185 190

Glu Ile Val Pro Val Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser

195 200 205

Glu Pro Ala Gly Ile Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His

210 215 220

Trp Asp Thr Tyr Thr Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp

225 230 235 240

Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp

245 250 255

Ser Lys Gln Thr Ala His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr

260 265 270

Asn Arg Gln Phe Leu Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu

275 280 285

Arg

<210>119

<211>2128

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2098)

<223>RXA02653

<400>119

agacagagtg ttagtgcgtg gggcagctct cactttcatc gacatcactc gagtatgctc 60

accggccgta ttcattccaa taacccgcac agggaaacta atg ata ccg aag ccc 115

Met Ile Pro Lys Pro

1 5

gac gtg acc gac tta tat tta gag gac ctc tta aat gag ggt tcg gaa 163

Asp Val Thr Asp Leu Tyr Leu Glu Asp Leu Leu Asn Glu Gly Ser Glu

10 15 20

aag att cgg tcc gcc aag gat ctt tcc gaa ctt agg aca gtt cta aaa 211

Lys Ile Arg Ser Ala Lys Asp Leu Ser Glu Leu Arg Thr Val Leu Lys

25 30 35

gag gtt tcc tcc caa att cag gaa cga gct ggg aaa aaa gat gaa gaa 259

Glu Val Ser Ser Gln Ile Gln Glu Arg Ala Gly Lys Lys Asp Glu Glu

40 45 50

tgg gga atg ggg gcc act tgg cgg gag ctg tac ccc agc atc gtg gaa 307

Trp Gly Met Gly Ala Thr Trp Arg Glu Leu Tyr Pro Ser Ile Val Glu

55 60 65

cgc gct tcc tac gaa ggg cgt gac agc cta atc gga ttt gat cac tta 355

Arg Ala Ser Tyr Glu Gly Arg Asp Ser Leu Ile Gly Phe Asp His Leu

70 75 80 85

gcc cgg gaa atg gaa aga tta gcc ttc ggc cca cca tcc gaa agt ttt 403

Ala Arg Glu Met Glu Arg Leu Ala Phe Gly Pro Pro Ser Glu Ser Phe

90 95 100

gaa tac ctc caa gaa ctc gta aaa tcc gga gtg gta gac atc act cac 451

Glu Tyr Leu Gln Glu Leu Val Lys Ser Gly Val Val Asp Ile Thr His

105 110 115

ctg cat cgt ggc cgg gaa cca ctg aca gat tta gtt cgt gaa ctt gaa 499

Leu His Arg Gly Arg Glu Pro Leu Thr Asp Leu Val Arg Glu Leu Glu

120 125 130

ata act gtg gtg ata gac gct gtt ctt ccc ccg ccg gga gta gtg cca 547

Ile Thr Val Val Ile Asp Ala Val Leu Pro Pro Pro Gly Val Val Pro

135 140 145

ggc aca ttg gtg cac aat ttg gta aaa gag gga tat gcc aga atg cgt 595

Gly Thr Leu Val His Asn Leu Val Lys Glu Gly Tyr Ala Arg Met Arg

150 155 160 165

cct ggg act cgg ggg tta gat gta gcg gct gac ggc acc gtt caa ggg 643

Pro Gly Thr Arg Gly Leu Asp Val Ala Ala Asp Gly Thr Val Gln Gly

170 175 180

caa cga cat ttg gct gca gtc gga cgg atg acg gaa gat gtg gtt ttg 691

Gln Arg His Leu Ala Ala Val Gly Arg Met Thr Glu Asp Val Val Leu

185 190 195

ggt aat gac aca ttg tcg cga tca tta cat gac ata atc ccg aag tgg 739

Gly Asn Asp Thr Leu Ser Arg Ser Leu His Asp Ile Ile Pro Lys Trp

200 205 210

gct cgt cga gtt atc cgc gac gcg agc acg tat ccc gat agg gta cat 787

Ala Arg Arg Val Ile Arg Asp Ala Ser Thr Tyr Pro Asp Arg Val His

215 220 225

ggt act cca ccg ctt ccg gca cgg ttg gaa ccc tgg gcg gaa aag ctc 835

Gly Thr Pro Pro Leu Pro Ala Arg Leu Glu Pro Trp Ala Glu Lys Leu

230 235 240 245

act tca gat ccg gcc aca tgc cgc cac ctg att gaa gaa ttc ggg agt 883

Thr Ser Asp Pro Ala Thr Cys Arg His Leu Ile Glu Glu Phe Gly Ser

250 255 260

cct gtg aat gta ctc cat tca ggt tct atg cct cgt aat ata aat gag 931

Pro Val Asn Val Leu His Ser Gly Ser Met Pro Arg Asn Ile Asn Glu

265 270 275

ttg gtt gac gcc ggc att cag atg ggg gtg gat act cga ata ttt ttt 979

Leu Val Asp Ala Gly Ile Gln Met Gly Val Asp Thr Arg Ile Phe Phe

280 285 290

gcc cgc aaa gcg aat aag ggt ctt acc ttc gtt gat gcc gtt aaa gac 1027

Ala Arg Lys Ala Asn Lys Gly Leu Thr Phe Val Asp Ala Val Lys Asp

295 300 305

acc ggt cat ggt gta gat gta gcc agt gaa cga gag tta tct cag gtg 1075

Thr Gly His Gly Val Asp Val Ala Ser Glu Arg Glu Leu Ser Gln Val

310 315 320 325

ctt aat cgt gga gtc cca gga gag cgg atc att cta tcc gca gct atc 1123

Leu Asn Arg Gly Val Pro Gly Glu Arg Ile Ile Leu Ser Ala Ala Ile

330 335 340

aaa ccg gac aga cta ttg gca tta gcg atc gaa aat ggc gtg atc atc 1171

Lys Pro Asp Arg Leu Leu Ala Leu Ala Ile Glu Asn Gly Val Ile Ile

345 350 355

tct gtg gat tcg cgt gat gaa tta gat cgc att tcg gct ttg gtt ggt 1219

Ser Val Asp Ser Arg Asp Glu Leu Asp Arg Ile Ser Ala Leu Val Gly

360 365 370

gac cgc gtt gca cga gtt gcg cct aga gta gct cca gat cct gca gtc 1267

Asp Arg Val Ala Arg Val Ala Pro Arg Val Ala Pro Asp Pro Ala Val

375 380 385

tta cct cca act aga ttt ggt gag cgt gct gca gac tgg ggt aat cgg 1315

Leu Pro Pro Thr Arg Phe Gly Glu Arg Ala Ala Asp Trp Gly Asn Arg

390 395 400 405

ctt acc gag gtg ata ccc ggc gtg gat att gtg ggt ctt cac gtt cac 1363

Leu Thr Glu Val Ile Pro Gly Val Asp Ile Val Gly Leu His Val His

410 415 420

ctc cat ggc tat gct gca aaa gac cgt gct ctg gct ctg cag gaa tgt 1411

Leu His Gly Tyr Ala Ala Lys Asp Arg Ala Leu Ala Leu Gln Glu Cys

425 430 435

tgc caa ctc gtc gat tct ctc aga gaa tgc ggg cat tcc cca cag ttt 1459

Cys Gln Leu Val Asp Ser Leu Arg Glu Cys Gly His Ser Pro Gln Phe

440 445 450

att gac ctt gga gga ggg gtg cct atg agc tac att gaa tct gag gaa 1507

Ile Asp Leu Gly Gly Gly Val Pro Met Ser Tyr Ile Glu Ser Glu Glu

455 460 465

gat tgg atc cgt tat caa tcc gct aaa tct gcg act tca gcc ggg tat 1555

Asp Trp Ile Arg Tyr Gln Ser Ala Lys Ser Ala Thr Ser Ala Gly Tyr

470 475 480 485

gcc gaa tcc ttt acg tgg aaa gac gat ccg tta tct aat acg tac ccg 1603

Ala Glu Ser Phe Thr Trp Lys Asp Asp Pro Leu Ser Asn Thr Tyr Pro

490 495 500

ttc tat cag acc cca gtg cgc ggt aat tgg ttg aaa gac gtg ctt tct 1651

Phe Tyr Gln Thr Pro Val Arg Gly Asn Trp Leu Lys Asp Val Leu Ser

505 510 515

aag ggg gta gct cag atg ctc att gac cgg gga ttg cgg tta cac cta 1699

Lys Gly Val Ala Gln Met Leu Ile Asp Arg Gly Leu Arg Leu His Leu

520 525 530

gag cct ggt cga agt tta cta gat ggg tgt ggc gtc act ctt gcc gaa 1747

Glu Pro Gly Arg Ser Leu Leu Asp Gly Cys Gly Val Thr Leu Ala Glu

535 540 545

gtt gct ttt gtg aaa acc cga agt gac ggg ttg cct cta gtg gga ctg 1795

Val Ala Phe Val Lys Thr Arg Ser Asp Gly Leu Pro Leu Val Gly Leu

550 555 560 565

gct atg aac cga acg cag tgc cgg act aca tcc gat gat ttt ctc att 1843

Ala Met Asn Arg Thr Gln Cys Arg Thr Thr Ser Asp Asp Phe Leu Ile

570 575 580

gat ccc ctg cat atc act gac ggt gat gta ggc gag gaa atc gaa gca 1891

Asp Pro Leu His Ile Thr Asp Gly Asp Val Gly Glu Glu Ile Glu Ala

585 590 595

tat cta gtg ggt gcc tac tgc atc gaa gat gag ctg att tta cgc cgg 1939

Tyr Leu Val Gly Ala Tyr Cys Ile Glu Asp Glu Leu Ile Leu Arg Arg

600 605 610

cga atc cgc ttc ccg aga gga gtc aaa cca gga gat atc atc gga att 1987

Arg Ile Arg Phe Pro Arg Gly Val Lys Pro Gly Asp Ile Ile Gly Ile

615 620 625

cct aac acc gca gga tac ttc atg cat atc ttg gaa agt gca tcg cac 2035

Pro Asn Thr Ala Gly Tyr Phe Met His Ile Leu Glu Ser Ala Ser His

630 635 640 645

caa atc ccg ttg gcg aaa aat gta gtg tgg ccg gag ggg cag tta gac 2083

Gln Ile Pro Leu Ala Lys Asn Val Val Trp Pro Glu Gly Gln Leu Asp

650 655 660

gat atc gat gcg gat taagacataa ccattcgcta atctttcgac 2128

Asp Ile Asp Ala Asp

665

<210>120

<211>666

<212>PRT

<213>谷氨酸棒状杆菌

<400>120

Met Ile Pro Lys Pro Asp Val Thr Asp Leu Tyr Leu Glu Asp Leu Leu

1 5 10 15

Asn Glu Gly Ser Glu Lys Ile Arg Ser Ala Lys Asp Leu Ser Glu Leu

20 25 30

Arg Thr Val Leu Lys Glu Val Ser Ser Gln Ile Gln Glu Arg Ala Gly

35 40 45

Lys Lys Asp Glu Glu Trp Gly Met Gly Ala Thr Trp Arg Glu Leu Tyr

50 55 60

Pro Ser Ile Val Glu Arg Ala Ser Tyr Glu Gly Arg Asp Ser Leu Ile

65 70 75 80

Gly Phe Asp His Leu Ala Arg Glu Met Glu Arg Leu Ala Phe Gly Pro

85 90 95

Pro Ser Glu Ser Phe Glu Tyr Leu Gln Glu Leu Val Lys Ser Gly Val

100 105 110

Val Asp Ile Thr His Leu His Arg Gly Arg Glu Pro Leu Thr Asp Leu

115 120 125

Val Arg Glu Leu Glu Ile Thr Val Val Ile Asp Ala Val Leu Pro Pro

130 135 140

Pro Gly Val Val Pro Gly Thr Leu Val His Asn Leu Val Lys Glu Gly

145 150 155 160

Tyr Ala Arg Met Arg Pro Gly Thr Arg Gly Leu Asp Val Ala Ala Asp

165 170 175

Gly Thr Val Gln Gly Gln Arg His Leu Ala Ala Val Gly Arg Met Thr

180 185 190

Glu Asp Val Val Leu Gly Asn Asp Thr Leu Ser Arg Ser Leu His Asp

195 200 205

Ile Ile Pro Lys Trp Ala Arg Arg Val Ile Arg Asp Ala Ser Thr Tyr

210 215 220

Pro Asp Arg Val His Gly Thr Pro Pro Leu Pro Ala Arg Leu Glu Pro

225 230 235 240

Trp Ala Glu Lys Leu Thr Ser Asp Pro Ala Thr Cys Arg His Leu Ile

245 250 255

Glu Glu Phe Gly Ser Pro Val Asn Val Leu His Ser Gly Ser Met Pro

260 265 270

Arg Asn Ile Asn Glu Leu Val Asp Ala Gly Ile Gln Met Gly Val Asp

275 280 285

Thr Arg Ile Phe Phe Ala Arg Lys Ala Asn Lys Gly Leu Thr Phe Val

290 295 300

Asp Ala Val Lys Asp Thr Gly His Gly Val Asp Val Ala Ser Glu Arg

305 310 315 320

Glu Leu Ser Gln Val Leu Asn Arg Gly Val Pro Gly Glu Arg Ile Ile

325 330 335

Leu Ser Ala Ala Ile Lys Pro Asp Arg Leu Leu Ala Leu Ala Ile Glu

340 345 350

Asn Gly Val Ile Ile Ser Val Asp Ser Arg Asp Glu Leu Asp Arg Ile

355 360 365

Ser Ala Leu Val Gly Asp Arg Val Ala Arg Val Ala Pro Arg Val Ala

370 375 380

Pro Asp Pro Ala Val Leu Pro Pro Thr Arg Phe Gly Glu Arg Ala Ala

385 390 395 400

Asp Trp Gly Asn Arg Leu Thr Glu Val Ile Pro Gly Val Asp Ile Val

405 410 415

Gly Leu His Val His Leu His Gly Tyr Ala Ala Lys Asp Arg Ala Leu

420 425 430

Ala Leu Gln Glu Cys Cys Gln Leu Val Asp Ser Leu Arg Glu Cys Gly

435 440 445

His Ser Pro Gln Phe Ile Asp Leu Gly Gly Gly Val Pro Met Ser Tyr

450 455 460

Ile Glu Ser Glu Glu Asp Trp Ile Arg Tyr Gln Ser Ala Lys Ser Ala

465 470 475 480

Thr Ser Ala Gly Tyr Ala Glu Ser Phe Thr Trp Lys Asp Asp Pro Leu

485 490 495

Ser Asn Thr Tyr Pro Phe Tyr Gln Thr Pro Val Arg Gly Asn Trp Leu

500 505 510

Lys Asp Val Leu Ser Lys Gly Val Ala Gln Met Leu Ile Asp Arg Gly

515 520 525

Leu Arg Leu His Leu Glu Pro Gly Arg Ser Leu Leu Asp Gly Cys Gly

530 535 540

Val Thr Leu Ala Glu Val Ala Phe Val Lys Thr Arg Ser Asp Gly Leu

545 550 555 560

Pro Leu Val Gly Leu Ala Met Asn Arg Thr Gln Cys Arg Thr Thr Ser

565 570 575

Asp Asp Phe Leu Ile Asp Pro Leu His Ile Thr Asp Gly Asp Val Gly

580 585 590

Glu Glu Ile Glu Ala Tyr Leu Val Gly Ala Tyr Cys Ile Glu Asp Glu

595 600 605

Leu Ile Leu Arg Arg Arg Ile Arg Phe Pro Arg Gly Val Lys Pro Gly

610 615 620

Asp Ile Ile Gly Ile Pro Asn Thr Ala Gly Tyr Phe Met His Ile Leu

625 630 635 640

Glu Ser Ala Ser His Gln Ile Pro Leu Ala Lys Asn Val Val Trp Pro

645 650 655

Glu Gly Gln Leu Asp Asp Ile Asp Ala Asp

660 665

<210>121

<211>1468

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1438)

<223>RXA02754

<400>121

attatgaagc catcggagtt ggtgtggcct acaagggtga tcatgcgtgg atagtggtgg 60

agttcactgt agctcccgct gattccgtag aatcaacaga gtg aat acc aat ccg 115

Val Asn Thr Asn Pro

1 5

tct gaa ttc tcc tca aac cgt tca aca gct ctc ctt act gat aaa tat 163

Ser Glu Phe Ser Ser Asn Arg Ser Thr Ala Leu Leu Thr Asp Lys Tyr

10 15 20

gag ctg acc atg ctt caa gca gcg ctc gct gat ggt tct gca gaa cgc 211

Glu Leu Thr Met Leu Gln Ala Ala Leu Ala Asp Gly Ser Ala Glu Arg

25 30 35

ccc tca acg ttt gag gtc ttt agc cgc cgc ctc ccc aac gag cgc cga 259

Pro Ser Thr Phe Glu Val Phe Ser Arg Arg Leu Pro Asn Glu Arg Arg

40 45 50

tac ggt gtc gtc gca gga aca gca cga gtg ctg aag gcg att cgt gac 307

Tyr Gly Val Val Ala Gly Thr Ala Arg Val Leu Lys Ala Ile Arg Asp

55 60 65

ttt gta ttc aca gag gaa caa ctc gcc gat ctt gac ttt tta gac gac 355

Phe Val Phe Thr Glu Glu Gln Leu Ala Asp Leu Asp Phe Leu Asp Asp

70 75 80 85

cgt acc ctg gaa tac ctc cgc aac tac cga ttc acc ggc caa gtt gat 403

Arg Thr Leu Glu Tyr Leu Arg Asn Tyr Arg Phe Thr Gly Gln Val Asp

90 95 100

ggc tac cgc gaa ggc gaa atc tac ttc ccg cag tcc cct ctt ctg act 451

Gly Tyr Arg Glu Gly Glu Ile Tyr Phe Pro Gln Ser Pro Leu Leu Thr

105 110 115

gtg cgt ggc acg ttt gca gaa tgc gtc atc cta gaa act gtc att ttg 499

Val Arg Gly Thr Phe Ala Glu Cys Val Ile Leu Glu Thr Val Ile Leu

120 125 130

tcc atc atg aat gca gat tct gcc gtc gct tcc gcc gct gcg cgc atg 547

Ser Ile Met Asn Ala Asp Ser Ala Val Ala Ser Ala Ala Ala Arg Met

135 140 145

gtc acc gca gct gat ggt cgc ccc atc atc gaa atg gga tcc agg cgc 595

Val Thr Ala Ala Asp Gly Arg Pro Ile Ile Glu Met Gly Ser Arg Arg

150 155 160 165

acc cac gaa tac tcg gca gtc acc gca tcc cgc gca gca tac ctc gct 643

Thr His Glu Tyr Ser Ala Val Thr Ala Ser Arg Ala Ala Tyr Leu Ala

170 175 180

gga ttc tcc acc acc tcc aac ctc gag gcg gcc tac cgc tac gga att 691

Gly Phe Ser Thr Thr Ser Asn Leu Glu Ala Ala Tyr Arg Tyr Gly Ile

185 190 195

cca gca tcc gga acc tcc gcc cac gca tgg act ttg ctg cac atc aac 739

Pro Ala Ser Gly Thr Ser Ala His Ala Trp Thr Leu Leu His Ile Asn

200 205 210

gat gac ggc acc ccc aac gaa gca gca gct ttc aaa gca cag gtt gaa 787

Asp Asp Gly Thr Pro Asn Glu Ala Ala Ala Phe Lys Ala Gln Val Glu

215 220 225

tcc ctc ggc gtg gac acc acc ttg ctg gta gat act tat gac atc acc 835

Ser Leu Gly Val Asp Thr Thr Leu Leu Val Asp Thr Tyr Asp Ile Thr

230 235 240 245

caa ggt gtg gcc acc gcc att gaa gtt gca ggt cca gac ctt ggt ggc 883

Gln Gly Val Ala Thr Ala Ile Glu Val Ala Gly Pro Asp Leu Gly Gly

250 255 260

gta cgt atc gac tcc ggc gac cta ggt gtg ctt gcc cga aag gtc cgc 931

Val Arg Ile Asp Ser Gly Asp Leu Gly Val Leu Ala Arg Lys Val Arg

265 270 275

aag cag ctc gac gat ctc aac gcc cac aac acc aag att gtg gtc tcc 979

Lys Gln Leu Asp Asp Leu Asn Ala His Asn Thr Lys Ile Val Val Ser

280 285 290

tcc gac ctg gat gaa ttc gcc atc gcg ggt ctt cgc ggc gaa cca gtt 1027

Ser Asp Leu Asp Glu Phe Ala Ile Ala Gly Leu Arg Gly Glu Pro Val

295 300 305

gac gtc ttt ggc gtt ggc acc tcc gtt gtc aca ggt tct ggc gca cca 1075

Asp Val Phe Gly Val Gly Thr Ser Val Val Thr Gly Ser Gly Ala Pro

310 315 320 325

acc gct ggc ctc gtg tac aag atc gtg gaa gtt gcc ggt cac cct gtg 1123

Thr Ala Gly Leu Val Tyr Lys Ile Val Glu Val Ala Gly His Pro Val

330 335 340

gcc aag cgt tcc cga aac aag gaa agc tac ggt ggt ggc aag aag gct 117l

Ala Lys Arg Ser Arg Asn Lys Glu Ser Tyr Gly Gly Gly Lys Lys Ala

345 350 355

gtg cgc acc cac cgc aag tcc ggt acc gca atc gaa gaa atc gtc tac 1219

Val Arg Thr His Arg Lys Ser Gly Thr Ala Ile Glu Glu Ile Val Tyr

360 365 370

cca ttc aat gcc gaa gca cca gat act gga aag ctc gac act ttg agc 1267

Pro Phe Asn Ala Glu Ala Pro Asp Thr Gly Lys Leu Asp Thr Leu Ser

375 380 385

ctg acc atc cca ttg atg cgc gac ggt gaa atc gtt cca ggt ttg cct 1315

Leu Thr Ile Pro Leu Met Arg Asp Gly Glu Ile Val Pro Gly Leu Pro

390 395 400 405

act ttg gaa gat tcc cga gcg tat ttg gcc aag caa ttg gtc tct tta 1363

Thr Leu Glu Asp Ser Arg Ala Tyr Leu Ala Lys Gln Leu Val Ser Leu

410 415 420

cca tgg gaa ggc ctt gca ctg tct cgc gat gag cct gtt ttg cac act 1411

Pro Trp Glu Gly Leu Ala Leu Ser Arg Asp Glu Pro Val Leu His Thr

425 430 435

cgt ttc gtg ggt ttc ccg ccg gcc gct tagacaattc ggtctcacca 1458

Arg Phe Val Gly Phe Pro Pro Ala Ala

440 445

aaccacagtg 1468

<210>122

<211>446

<212>PRT

<213>谷氨酸棒状杆菌

<400>122

Val Asn Thr Asn Pro Ser Glu Phe Ser Ser Asn Arg Ser Thr Ala Leu

1 5 10 15

Leu Thr Asp Lys Tyr Glu Leu Thr Met Leu Gln Ala Ala Leu Ala Asp

20 25 30

Gly Ser Ala Glu Arg Pro Ser Thr Phe Glu Val Phe Ser Arg Arg Leu

35 40 45

Pro Asn Glu Arg Arg Tyr Gly Val Val Ala Gly Thr Ala Arg Val Leu

50 55 60

Lys Ala Ile Arg Asp Phe Val Phe Thr Glu Glu Gln Leu Ala Asp Leu

65 70 75 80

Asp Phe Leu Asp Asp Arg Thr Leu Glu Tyr Leu Arg Asn Tyr Arg Phe

85 90 95

Thr Gly Gln Val Asp Gly Tyr Arg Glu Gly Glu Ile Tyr Phe Pro Gln

100 105 110

Ser Pro Leu Leu Thr Val Arg Gly Thr Phe Ala Glu Cys Val Ile Leu

115 120 125

Glu Thr Val Ile Leu Ser Ile Met Asn Ala Asp Ser Ala Val Ala Ser

130 135 140

Ala Ala Ala Arg Met Val Thr Ala Ala Asp Gly Arg Pro Ile Ile Glu

145 150 155 160

Met Gly Ser Arg Arg Thr His Glu Tyr Ser Ala Val Thr Ala Ser Arg

165 170 175

Ala Ala Tyr Leu Ala Gly Phe Ser Thr Thr Ser Asn Leu Glu Ala Ala

180 185 190

Tyr Arg Tyr Gly Ile Pro Ala Ser Gly Thr Ser Ala His Ala Trp Thr

195 200 205

Leu Leu His Ile Asn Asp Asp Gly Thr Pro Asn Glu Ala Ala Ala Phe

210 215 220

Lys Ala Gln Val Glu Ser Leu Gly Val Asp Thr Thr Leu Leu Val Asp

225 230 235 240

Thr Tyr Asp Ile Thr Gln Gly Val Ala Thr Ala Ile Glu Val Ala Gly

245 250 255

Pro Asp Leu Gly Gly Val Arg Ile Asp Ser Gly Asp Leu Gly Val Leu

260 265 270

Ala Arg Lys Val Arg Lys Gln Leu Asp Asp Leu Asn Ala His Asn Thr

275 280 285

Lys Ile Val Val Ser Ser Asp Leu Asp Glu Phe Ala Ile Ala Gly Leu

290 295 300

Arg Gly Glu Pro Val Asp Val Phe Gly Val Gly Thr Ser Val Val Thr

305 310 315 320

Gly Ser Gly Ala Pro Thr Ala Gly Leu Val Tyr Lys Ile Val Glu Val

325 330 335

Ala Gly His Pro Val Ala Lys Arg Ser Arg Asn Lys Glu Ser Tyr Gly

340 345 350

Gly Gly Lys Lys Ala Val Arg Thr His Arg Lys Ser Gly Thr Ala Ile

355 360 365

Glu Glu Ile Val Tyr Pro Phe Asn Ala Glu Ala Pro Asp Thr Gly Lys

370 375 380

Leu Asp Thr Leu Ser Leu Thr Ile Pro Leu Met Arg Asp Gly Glu Ile

385 390 395 400

Val Pro Gly Leu Pro Thr Leu Glu Asp Ser Arg Ala Tyr Leu Ala Lys

405 410 415

Gln Leu Val Ser Leu Pro Trp Glu Gly Leu Ala Leu Ser Arg Asp Glu

420 425 430

Pro Val Leu His Thr Arg Phe Val Gly Phe Pro Pro Ala Ala

435 440 445

<210>123

<211>1468

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1438)

<223>RXA02758

<400>123

ggctggtgct tttcatattc cgacttgggg cacccctgaa tacatctcac ccaattcccc 60

ataactagac aattgcccag caacgactga taagtctcca atg tcg tgt tcc gcg 115

Met Ser Cys Ser Ala

1 5

ctc aga cat gag aca att gtt gcc gtg act gaa ctc atc cag aat gaa 163

Leu Arg His Glu Thr Ile Val Ala Val Thr Glu Leu Ile Gln Asn Glu

10 15 20

tcc caa gaa atc gct gag ctg gaa gcc ggc cag cag gtt gca ttg cgt 211

Ser Gln Glu Ile Ala Glu Leu Glu Ala Gly Gln Gln Val Ala Leu Arg

25 30 35

gaa ggt tat ctt cct gcg gtg atc aca gtg agc ggt aaa gac cgc cca 259

Glu Gly Tyr Leu Pro Ala Val Ile Thr Val Ser Gly Lys Asp Arg Pro

40 45 50

ggt gtg act gcc gcg ttc ttt agg gtc ttg tcc gct aat cag gtt cag 307

Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala Asn Gln Val Gln

55 60 65

gtc ttg gac gtt gag cag tca atg ttc cgt ggc ttt ttg aac ttg gcg 355

Val Leu Asp Val Glu Gln Ser Met Phe Arg Gly Phe Leu Asn Leu Ala

70 75 80 85

gcg ttt gtg ggt atc gca cct gag cgt gtc gag acc gtc acc aca ggc 403

Ala Phe Val Gly Ile Ala Pro Glu Arg Val Glu Thr Val Thr Thr Gly

90 95 100

ctg act gac acc ctc aag gtg cat gga cag tcc gtg gtg gtg gag ctg 451

Leu Thr Asp Thr Leu Lys Val His Gly Gln Ser Val Val Val Glu Leu

105 110 115

cag gaa act gtg cag tcg tcc cgt cct cgt tct tcc cat gtt gtt gtg 499

Gln Glu Thr Val Gln Ser Ser Arg Pro Arg Ser Ser His Val Val Val

120 125 130

gtg ttg ggt gat ccg gtt gat gcg ttg gat att tcc cgc att ggt cag 547

Val Leu Gly Asp Pro Val Asp Ala Leu Asp Ile Ser Arg Ile Gly Gln

135 140 145

acc ctg gcg gat tac gat gcc aac att gac acc att cgt ggt att tcg 595

Thr Leu Ala Asp Tyr Asp Ala Asn Ile Asp Thr Ile Arg Gly Ile Ser

150 155 160 165

gat tac cct gtg acc ggc ctg gag ctg aag gtg act gtg ccg gat gtc 643

Asp Tyr Pro Val Thr Gly Leu Glu Leu Lys Val Thr Val Pro Asp Val

170 175 180

agc cct ggt ggt ggt gaa gcg atg cgt aag gcg ctt gct gct ctt acc 691

Ser Pro Gly Gly Gly Glu Ala Met Arg Lys Ala Leu Ala Ala Leu Thr

185 190 195

tct gag ctg aat gtg gat att gcg att gag cgt tct ggt ttg ctg cgt 739

Ser Glu Leu Asn Val Asp Ile Ala Ile Glu Arg Ser Gly Leu Leu Arg

200 205 210

cgt tct aag cgt ctg gtg tgc ttc gat tgt gat tcc acg ttg atc act 787

Arg Ser Lys Arg Leu Val Cys Phe Asp Cys Asp Ser Thr Leu Ile Thr

215 220 225

ggt gag gtc att gag atg ctg gcg gct cac gcg ggc aag gaa gct gaa 835

Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly Lys Glu Ala Glu

230 235 240 245

gtt gcg gca gtt act gag cgt gcg atg cgc ggt gag ctc gat ttc gag 883

Val Ala Ala Val Thr Glu Arg Ala Met Arg Gly Glu Leu Asp Phe Glu

250 255 260

gag tct ctg cgt gag cgt gtg aag gcg ttg gct ggt ttg gat gcg tcg 931

Glu Ser Leu Arg Glu Arg Val Lys Ala Leu Ala Gly Leu Asp Ala Ser

265 270 275

gtg atc gat gag gtc gct gcc gct att gag ctg acc cct ggt gcg cgc 979

Val Ile Asp Glu Val Ala Ala Ala Ile Glu Leu Thr Pro Gly Ala Arg

280 285 290

acc acg atc cgt acg ctg aac cgc atg ggt tac cag acc gct gtt gtt 1027

Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr Gln Thr Ala Val Val

295 300 305

tcc ggt ggt ttc atc cag gtg ttg gaa ggt ttg gct gag gag ttg gag 1075

Ser Gly Gly Phe Ile Gln Val Leu Glu Gly Leu Ala Glu Glu Leu Glu

310 315 320 325

ttg gat tat gtc cgc gcc aac act ttg gaa atc gtt gat ggc aag ctg 1123

Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val Asp Gly Lys Leu

330 335 340

acc ggc aac gtc acc gga aag atc gtt gac cgc gct gcg aag gct gag 1171

Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg Ala Ala Lys Ala Glu

345 350 355

ttc ctc cgt gag ttc gct gcg gat tct ggc ctg aag atg tac cag act 1219

Phe Leu Arg Glu Phe Ala Ala Asp Ser Gly Leu Lys Met Tyr Gln Thr

360 365 370

gtc gct gtc ggt gat ggc gct aat gac atc gat atg ctc tcc gct gcg 1267

Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met Leu Ser Ala Ala

375 380 385

ggt ctg ggt gtt gct ttc aac gcg aag cct gcg ctg aag gag att gcg 1315

Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala Leu Lys Glu Ile Ala

390 395 400 405

gat act tcc gtg aac cac cca ttc ctc gac gag gtt ttg cac atc atg 1363

Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val Leu His Ile Met

410 415 420

ggc att tcc cgc gac gag atc gat ctg gcg gat cag gaa gac ggc act 1411

Gly Ile Ser Arg Asp Glu Ile Asp Leu Ala Asp Gln Glu Asp Gly Thr

425 430 435

ttc cac cgc gtt cca ttg acc aat gcc taaagattcg cttctcgacg 1458

Phe His Arg Val Pro Leu Thr Asn Ala

440 445

cccacctcct 1468

<210>124

<211>446

<212>PRT

<213>谷氨酸棒状杆菌

<400>124

Met Ser Cys Ser Ala Leu Arg His Glu Thr Ile Val Ala Val Thr Glu

1 5 10 15

Leu Ile Gln Asn Glu Ser Gln Glu Ile Ala Glu Leu Glu Ala Gly Gln

20 25 30

Gln Val Ala Leu Arg Glu Gly Tyr Leu Pro Ala Val Ile Thr Val Ser

35 40 45

Gly Lys Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser

50 55 60

Ala Asn Gln Val Gln Val Leu Asp Val Glu Gln Ser Met Phe Arg Gly

65 70 75 80

Phe Leu Asn Leu Ala Ala Phe Val Gly Ile Ala Pro Glu Arg Val Glu

85 90 95

Thr Val Thr Thr Gly Leu Thr Asp Thr Leu Lys Val His Gly Gln Ser

100 105 110

Val Val Val Glu Leu Gln Glu Thr Val Gln Ser Ser Arg Pro Arg Ser

115 120 125

Ser His Val Val Val Val Leu Gly Asp Pro Val Asp Ala Leu Asp Ile

130 135 140

Ser Arg Ile Gly Gln Thr Leu Ala Asp Tyr Asp Ala Asn Ile Asp Thr

145 150 155 160

Ile Arg Gly Ile Ser Asp Tyr Pro Val Thr Gly Leu Glu Leu Lys Val

165 170 175

Thr Val Pro Asp Val Ser Pro Gly Gly Gly Glu Ala Met Arg Lys Ala

180 185 190

Leu Ala Ala Leu Thr Ser Glu Leu Asn Val Asp Ile Ala Ile Glu Arg

195 200 205

Ser Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Cys Phe Asp Cys Asp

210 215 220

Ser Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala

225 230 235 240

Gly Lys Glu Ala Glu Val Ala Ala Val Thr Glu Arg Ala Met Arg Gly

245 250 255

Glu Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Lys Ala Leu Ala

260 265 270

Gly Leu Asp Ala Ser Val Ile Asp Glu Val Ala Ala Ala Ile Glu Leu

275 280 285

Thr Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr

290 295 300

Gln Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Gly Leu

305 310 315 320

Ala Glu Glu Leu Glu Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile

325 330 335

Val Asp Gly Lys Leu Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg

340 345 350

Ala Ala Lys Ala Glu Phe Leu Arg Glu Phe Ala Ala Asp Ser Gly Leu

355 360 365

Lys Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp

370 375 380

Met Leu Ser Ala Ala Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala

385 390 395 400

Leu Lys Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu

405 410 415

Val Leu His Ile Met Gly Ile Ser Arg Asp Glu Ile Asp Leu Ala Asp

420 425 430

Gln Glu Asp Gly Thr Phe His Arg Val Pro Leu Thr Asn Ala

435 440 445

<210>125

<211>1297

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1267)

<223>RXA02790

<400>125

cagtagaaat ccttcaaacc tggttggatg ctcgcacccg agcccttgaa ccacaatcca 60

cagacaccca agatttcgac gagaagggaa atttcccagg atg aac caa atc cga 115

Met Asn Gln Ile Arg

1 5

aac cgc cgg atg gag ccc gtc tac gta aag cgc cgc caa cgg ttt att 163

Asn Arg Arg Met Glu Pro Val Tyr Val Lys Arg Arg Gln Arg Phe Ile

10 15 20

gcc gtg acg atc gct tca ctc atc ctc att atc ggt gcc atc atc tat 211

Ala Val Thr Ile Ala Ser Leu Ile Leu Ile Ile Gly Ala Ile Ile Tyr

25 30 35

atc ggt gta gcc acc tca aac cgg acg cca cat gac tat gaa ggc tcc 259

Ile Gly Val Ala Thr Ser Asn Arg Thr Pro His Asp Tyr Glu Gly Ser

40 45 50

gga aac ggt gtg gtt cag ctg gtc gaa atc cct gaa ggt tcc tcc ata 307

Gly Asn Gly Val Val Gln Leu Val Glu Ile Pro Glu Gly Ser Ser Ile

55 60 65

tca gag ctc ggc cca gag ttg gaa gaa cga gat atc gtg gcc acc aac 355

Ser Glu Leu Gly Pro Glu Leu Glu Glu Arg Asp Ile Val Ala Thr Asn

70 75 80 85

tca gcg ttc caa aca gcg gcc agc aac aac ccc aac gcg ggt agt gta 403

Ser Ala Phe Gln Thr Ala Ala Ser Asn Asn Pro Asn Ala Gly Ser Val

90 95 100

cag cca ggt ttc tac cgt ctg cag gaa caa atg aac gca gca gct gca 451

Gln Pro Gly Phe Tyr Arg Leu Gln Glu Gln Met Asn Ala Ala Ala Ala

105 110 115

gtg tcg gct ctg ctt gat cca gac aac cag gtt gat ctc ctc gac att 499

Val Ser Ala Leu Leu Asp Pro Asp Asn Gln Val Asp Leu Leu Asp Ile

120 125 130

cac ggc ggc gcc acc ttg atg gac gtc act gtt gtc ggc gga aac acc 547

His Gly Gly Ala Thr Leu Met Asp Val Thr Val Val GlyGly Asn Thr

135 140 145

cgc gcg gga atc tac tcc cag atc gca gcc gtg acc tgc acc gaa ggc 595

Arg Ala Gly Ile Tyr Ser Gln Ile Ala Ala Val Thr Cys Thr Glu Gly

150 155 160 165

tcc gcc aac tgc atc acc gct gag gat ttg cag cag gtt gcc tcc acc 643

Ser Ala Asn Cys Ile Thr Ala Glu Asp Leu Gln Gln Val Ala Ser Thr

170 175 180

gtg tcg cct gca gaa ttg ggt gtc cca gat tgg gca atc gct gct gtg 691

Val Ser Pro Ala Glu Leu Gly Val Pro Asp Trp Ala Ile Ala Ala Val

185 190 195

gaa gct cgc gga act gat cca aag cgc ctc gaa ggc ctg atc atg cct 739

Glu Ala Arg Gly Thr Asp Pro Lys Arg Leu Glu Gly Leu Ile Met Pro

200 205 210

ggc caa tac gtg gtg gat cca tcc aac gac gcc cag gga atc ctc acc 787

Gly Gln Tyr Val Val Asp Pro Ser Asn Asp Ala Gln Gly Ile Leu Thr

215 220 225

gat ctg atc acg cga tca gca aac cat ttc caa gaa acc gac atc acg 835

Asp Leu Ile Thr Arg Ser Ala Asn His Phe Gln Glu Thr Asp Ile Thr

230 235 240 245

ggc cgt gca gat gcc atc gga ctt act cca tat gag ctg gtc acc gca 883

Gly Arg Ala Asp Ala Ile Gly Leu Thr Pro Tyr Glu Leu Val Thr Ala

250 255 260

gca tct tta atc gag cgc gaa gca cca gca gga gat ttt gat aag gtc 931

Ala Ser Leu Ile Glu Arg Glu Ala Pro Ala Gly Asp Phe Asp Lys Val

265 270 275

gcc cgc gtc atc ttg aac cgt ctc gcc gag cca atg cag ctg caa ttc 979

Ala Arg Val Ile Leu Asn Arg Leu Ala Glu Pro Met Gln Leu Gln Phe

280 285 290

gac tcc acc gtc aac tac ggt ctg tct gaa caa gaa gta gca acc acc 1027

Asp Ser Thr Val Asn Tyr Gly Leu Ser Glu Gln Glu Val Ala Thr Thr

295 300 305

gac gaa gac cgt cag acc gtc acc cca tgg aac act tac gcc atg gac 1075

Asp Glu Asp Arg Gln Thr Val Thr Pro Trp Asn Thr Tyr Ala Met Asp

310 315 320 325

ggc ctg cca caa acc ccc atc gcc gca gta tcc acc gaa gca ctc caa 1123

Gly Leu Pro Gln Thr Pro Ile Ala Ala Val Ser Thr Glu Ala Leu Gln

330 335 340

gcc atg gaa aac cct gca gaa gga aac tgg ctg tac ttt gtc acc atc 1171

Ala Met Glu Asn Pro Ala Glu Gly Asn Trp Leu Tyr Phe Val Thr Ile

345 350 355

gac acc gat gga acc acc gtg ttc aac gac acc ttc gaa gag cac gaa 1219

Asp Thr Asp Gly Thr Thr Val Phe Asn Asp Thr Phe Glu Glu His Glu

360 365 370

gcc gac att gag caa gct ttg aac agt ggc gtt cta gac agc aac cga 1267

Ala Asp Ile Glu Gln Ala Leu Asn Ser Gly Val Leu Asp Ser Asn Arg

375 380 385

taaggatcag cgaataaaat tgggttctca 1297

<210>126

<211>389

<212>PRT

<213>谷氨酸棒状杆菌

<400>126

Met Asn Gln Ile Arg Asn Arg Arg Met Glu Pro Val Tyr Val Lys Arg

1 5 10 15

Arg Gln Arg Phe Ile Ala Val Thr Ile Ala Ser Leu Ile Leu Ile Ile

20 25 30

Gly Ala Ile Ile Tyr Ile Gly Val Ala Thr Ser Asn Arg Thr Pro His

35 40 45

Asp Tyr Glu Gly Ser Gly Asn Gly Val Val Gln Leu Val Glu Ile Pro

50 55 60

Glu Gly Ser Ser Ile Ser Glu Leu Gly Pro Glu Leu Glu Glu Arg Asp

65 70 75 80

Ile Val Ala Thr Asn Ser Ala Phe Gln Thr Ala Ala Ser Asn Asn Pro

85 90 95

Asn Ala Gly Ser Val Gln Pro Gly Phe Tyr Arg Leu Gln Glu Gln Met

100 105 110

Asn Ala Ala Ala Ala Val Ser Ala Leu Leu Asp Pro Asp Asn Gln Val

115 120 125

Asp Leu Leu Asp Ile His Gly Gly Ala Thr Leu Met Asp Val Thr Val

130 135 140

Val Gly Gly Asn Thr Arg Ala Gly Ile Tyr Ser Gln Ile Ala Ala Val

145 150 155 160

Thr Cys Thr Glu Gly Ser Ala Asn Cys Ile Thr Ala Glu Asp Leu Gln

165 170 175

Gln Val Ala Ser Thr Val Ser Pro Ala Glu Leu Gly Val Pro Asp Trp

180 185 190

Ala Ile Ala Ala Val Glu Ala Arg Gly Thr Asp Pro Lys Arg Leu Glu

195 200 205

Gly Leu Ile Met Pro Gly Gln Tyr Val Val Asp Pro Ser Asn Asp Ala

210 215 220

Gln Gly Ile Leu Thr Asp Leu Ile Thr Arg Ser Ala Asn His Phe Gln

225 230 235 240

Glu Thr Asp Ile Thr Gly Arg Ala Asp Ala Ile Gly Leu Thr Pro Tyr

245 250 255

Glu Leu Val Thr Ala Ala Ser Leu Ile Glu Arg Glu Ala Pro Ala Gly

260 265 270

Asp Phe Asp Lys Val Ala Arg Val Ile Leu Asn Arg Leu Ala Glu Pro

275 280 285

Met Gln Leu Gln Phe Asp Ser Thr Val Asn Tyr Gly Leu Ser Glu Gln

290 295 300

Glu Val Ala Thr Thr Asp Glu Asp Arg Gln Thr Val Thr Pro Trp Asn

305 310 315 320

Thr Tyr Ala Met Asp Gly Leu Pro Gln Thr Pro Ile Ala Ala Val Ser

325 330 335

Thr Glu Ala Leu Gln Ala Met Glu Asn Pro Ala Glu Gly Asn Trp Leu

340 345 350

Tyr Phe Val Thr Ile Asp Thr Asp Gly Thr Thr Val Phe Asn Asp Thr

355 360 365

Phe Glu Glu His Glu Ala Asp Ile Glu Gln Ala Leu Asn Ser Gly Val

370 375 380

Leu Asp Ser Asn Arg

385

<210>127

<211>883

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(853)

<223>RXA02912

<400>127

catcggtgtc ggttcactga tggtggccaa gaaggttgga taacagggtt aatcttgggt 60

gatattgaaa tgacgtattc ctgattgggc tgaaaaatct gtg aca tca cct gaa 115

Val Thr Ser Pro Glu

1 5

tta caa aac atc ctt aac aat tat tgg agc ggc agg gca gag gct tac 163

Leu Gln Asn Ile Leu Asn Asn Tyr Trp Ser Gly Arg Ala Glu Ala Tyr

10 15 20

cac ctc aac caa acc caa agc gag cgt gca caa ttt gaa cgc ccc atc 21l

His Leu Asn Gln Thr Gln Ser Glu Arg Ala Gln Phe Glu Arg Pro Ile

25 30 35

tgg gaa aag gtg tgg tcg aag gct ttg cct atc gtg tcg gaa gaa gcg 259

Trp Glu Lys Val Trp Ser Lys Ala Leu Pro Ile Val Ser Glu Glu Ala

40 45 50

gta aag gtt ctc gat ctt ggc tgt ggc gct ggt tat gtc acc cac ctt 307

Val Lys Val Leu Asp Leu Gly Cys Gly Ala Gly Tyr Val Thr His Leu

55 60 65

cta agc gat tgc gga tac gaa aca atc ggc gtt gat ggt tct gag gaa 355

Leu Ser Asp Cys Gly Tyr Glu Thr Ile Gly Val Asp Gly Ser Glu Glu

70 75 80 85

atg atc aat caa gct acg cag gag aat ggt ctt cgc agg tcg acg ggt 403

Met Ile Asn Gln Ala Thr Gln Glu Asn Gly Leu Arg Arg Ser Thr Gly

90 95 100

cgg gcg act gcc att ttt cag gtc ggg gat gcg cat gat ccc gag ttc 451

Arg Ala Thr Ala Ile Phe Gln Val Gly Asp Ala His Asp Pro Glu Phe

105 110 115

cgg gaa ggc tct ttt gat gcg ata acc agc cgg tat gtg ttg tgg act 499

Arg Glu Gly Ser Phe Asp Ala Ile Thr Ser Arg Tyr Val Leu Trp Thr

120 125 130

ctg ctg gat ccc cag gca gcg att aat cgt tgg gtg tct ttg cta aaa 547

Leu Leu Asp Pro Gln Ala Ala Ile Asn Arg Trp Val Ser Leu Leu Lys

135 140 145

cct ggt ggg gtt att gcg tgc gta gat gcg gct tgg tat ccg aag ggc 595

Pro Gly Gly Val Ile Ala Cys Val Asp Ala Ala Trp Tyr Pro Lys Gly

150 155 160 165

att gat gct ggc acg gaa gta gat tca gtg gat ggt ccg agt gct ttc 643

Ile Asp Ala Gly Thr Glu Val Asp Ser Val Asp Gly Pro Ser Ala Phe

170 175 180

gta gag acc tat acc ccg gaa ctt ttg agg aat ctt ccc atg tca acg 691

Val Glu Thr Tyr Thr Pro Glu Leu Leu Arg Asn Leu Pro Met Ser Thr

185 190 195

acc tcc act ggc cac aat ttc gca gag ctt ttc cac aac gct ggc ctc 739

Thr Ser Thr Gly His Asn Phe Ala Glu Leu Phe His Asn Ala Gly Leu

200 205 210

aaa gaa gtc aca ttg aca ccc att gag ggg ctg gct gaa ctt gac cag 787

Lys Glu Val Thr Leu Thr Pro Ile Glu Gly Leu Ala Glu Leu Asp Gln

215 220 225

cga ttt ggc ctc tca cca ggg cat gag tcg act ccg cag ttc cta ttc 835

Arg Phe Gly Leu Ser Pro Gly His Glu Ser Thr Pro Gln Phe Leu Phe

230 235 240 245

agg gga att aaa tcc agt tagtgctgtt ttaagcggtc gagaatctgg 883

Arg Gly Ile Lys Ser Ser

250

<210>128

<211>251

<212>PRT

<213>谷氨酸棒状杆菌

<400>128

Val Thr Ser Pro Glu Leu Gln Asn Ile Leu Asn Asn Tyr Trp Ser Gly

1 5 10 15

Arg Ala Glu Ala Tyr His Leu Asn Gln Thr Gln Ser Glu Arg Ala Gln

20 25 30

Phe Glu Arg Pro Ile Trp Glu Lys Val Trp Ser Lys Ala Leu Pro Ile

35 40 45

Val Ser Glu Glu Ala Val Lys Val Leu Asp Leu Gly Cys Gly Ala Gly

50 55 60

Tyr Val Thr His Leu Leu Ser Asp Cys Gly Tyr Glu Thr Ile Gly Val

65 70 75 80

Asp Gly Ser Glu Glu Met Ile Asn Gln Ala Thr Gln Glu Asn Gly Leu

85 90 95

Arg Arg Ser Thr Gly Arg Ala Thr Ala Ile Phe Gln Val Gly Asp Ala

100 105 110

His Asp Pro Glu Phe Arg Glu Gly Ser Phe Asp Ala Ile Thr Ser Arg

115 120 125

Tyr Val Leu Trp Thr Leu Leu Asp Pro Gln Ala Ala Ile Asn Arg Trp

130 135 140

Val Ser Leu Leu Lys Pro Gly Gly Val Ile Ala Cys Val Asp Ala Ala

145 150 155 160

Trp Tyr Pro Lys Gly Ile Asp Ala Gly Thr Glu Val Asp Ser Val Asp

165 170 175

Gly Pro Ser Ala Phe Val Glu Thr Tyr Thr Pro Glu Leu Leu Arg Asn

180 185 190

Leu Pro Met Ser Thr Thr Ser Thr Gly His Asn Phe Ala Glu Leu Phe

195 200 205

His Asn Ala Gly Leu Lys Glu Val Thr Leu Thr Pro Ile Glu Gly Leu

210 215 220

Ala Glu Leu Asp Gln Arg Phe Gly Leu Ser Pro Gly His Glu Ser Thr

225 230 235 240

Pro Gln Phe Leu Phe Arg Gly Ile Lys Ser Ser

245 250

<210>129

<211>1498

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1468)

<223>RXA02970

<400>129

aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag 60

ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag ggt 115

Leu Ala Leu Lys Gly

1 5

tac acc aac ttt gac ggt gaa ttc atc gaa ttc gga tct gtg caa gca 163

Tyr Thr Asn Phe Asp Gly Glu Phe Ile Glu Phe Gly Ser Val Gln Ala

10 15 20

aaa gaa gag gaa aaa cgg gca ttc gac aac gat cgc gcg cac gtt ttc 211

Lys Glu Glu Glu Lys Arg Ala Phe Asp Asn Asp Arg Ala His Val Phe

25 30 35

cac tcc tgg tcc gcg cag gac aaa atc agc ccc aaa gta tgg gca gct 259

His Ser Trp Ser Ala Gln Asp Lys Ile Ser Pro Lys Val Trp Ala Ala

40 45 50

gcc gaa ggt tcc acg ctg tac gac ttc gac ggc aac gcc ttc atc gac 307

Ala Glu Gly Ser Thr Leu Tyr Asp Phe Asp Gly Asn Ala Phe Ile Asp

55 60 65

atg ggt tcc caa ctt gtc tcg gca aac tta ggc cac aac aac cct cga 355

Met Gly Ser Gln Leu Val Ser Ala Asn Leu Gly His Asn Asn Pro Arg

70 75 80 85

tta gtt gag gcg atc cag cgc caa gca gcc cgg ttg acc aac atc aac 403

Leu Val Glu Ala Ile Gln Arg Gln Ala Ala Arg Leu Thr Asn Ile Asn

90 95 100

ccg gcc ttc ggc aat gat gtg cgc tct gat gtt gct gca aag atc gtg 451

Pro Ala Phe Gly Asn Asp Val Arg Ser Asp Val Ala Ala Lys Ile Val

105 110 115

tcg atg gcc cgt ggc gaa ttc tcc cac gtg ttt ttc acc aac ggc ggc 499

Ser Met Ala Arg Gly Glu Phe Ser His Val Phe Phe Thr Asn Gly Gly

120 125 130

gcc gac gcc atc gag cac tcc atc cgc atg gct cgc ctg cac acc gga 547

Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala Arg Leu His Thr Gly

135 140 145

cgc aac aaa att ctg tcc gca tac cgc agc tac cac ggc gca acc gga 595

Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr His Gly Ala Thr Gly

150 155 160 165

tcc gcg atg atg ctc acc ggc gaa cac cgc cgc ctg ggc aac ccc acc 643

Ser Ala Met Met Leu Thr Gly Glu His Arg Arg Leu Gly Asn Pro Thr

170 175 180

acc gac cca gat atc tac cac ttc tgg gca cca ttc ctg cac cac tcc 691

Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro Phe Leu His His Ser

185 190 195

tca ttc ttt gcc acc acc caa gaa gaa gaa tgc gaa cgc gca ctc aag 739

Ser Phe Phe Ala Thr Thr Gln Glu Glu Glu Cys Glu Arg Ala Leu Lys

200 205 210

cac ttg gaa gat gtc atc gcg ttt gaa ggt gct ggc atg atc gca gcg 787

His Leu Glu Asp Val Ile Ala Phe Glu Gly Ala Gly Met Ile Ala Ala

215 220 225

atc gtc ctg gag cca gtg gtg gga tca tca gga atc atc ctg cca cca 835

Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly Ile Ile Leu Pro Pro

230 235 240 245

gca ggt tac tta aat ggc gtg cgc gaa ctt tgc aac aag cac ggc atc 883

Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys Asn Lys His Gly Ile

250 255 260

ctc ttc atc gcc gac gaa gtc atg gtc gga ttc gga cgc acc gga aaa 931

Leu Phe Ile Ala Asp Glu Val Met Val Gly Phe Gly Arg Thr Gly Lys

265 270 275

ctg ttt gct tac gag cat gct ggc gac gat ttc cag cca gac atg atc 979

Leu Phe Ala Tyr Glu His Ala Gly Asp Asp Phe Gln Pro Asp Met Ile

280 285 290

acc ttc gcc aag ggt gtt aac gca ggt tac gcc cca ctc ggt ggc atc 1027

Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Ala Pro Leu Gly Gly Ile

295 300 305

gtg atg acc caa tca atc cgc gat acc ttc gga tca gag gca tac tcc 1075

Val Met Thr Gln Ser Ile Arg Asp Thr Phe Gly Ser Glu Ala Tyr Ser

310 315 320 325

ggc gga ctc acc tac tcc gga cac cca ctt gca gta gca ccc gcc aag 1123

Gly Gly Leu Thr Tyr Ser Gly His Pro Leu Ala Val Ala Pro Ala Lys

330 335 340

gca gcg ctg gag att tac gcg gaa gga gag atc att cca cgc gta gct 1171

Ala Ala Leu Glu Ile Tyr Ala Glu Gly Glu Ile Ile Pro Arg Val Ala

345 350 355

cga ctt ggc gct gaa ctg atc gaa cct cgc ctt cgt gaa cta gcg gaa 1219

Arg Leu Gly Ala Glu Leu Ile Glu Pro Arg Leu Arg Glu Leu Ala Glu

360 365 370

gaa aac gta gcg atc gct gac gtg cgg ggc atc gga ttc ttc tgg gca 1267

Glu Asn Val Ala Ile Ala Asp Val Arg Gly Ile Gly Phe Phe Trp Ala

375 380 385

gtg gag ttc aat gca gac gcc act gcc atg gct gcc ggt gct gca gaa 1315

Val Glu Phe Asn Ala Asp Ala Thr Ala Met Ala Ala Gly Ala Ala Glu

390 395 400 405

ttc aag gaa cgc ggc gtg tgg ccg atg atc tcc ggc aac cga ttc cac 1363

Phe Lys Glu Arg Gly Val Trp Pro Met Ile Ser Gly Asn Arg Phe His

410 415 420

atc gcg ccg ccg ctg acc acc act gat gac gaa ttg gta gca ctg ctg 1411

Ile Ala Pro Pro Leu Thr Thr Thr Asp Asp Glu Leu Val Ala Leu Leu

425 430 435

gac gcg gtg gaa gct gca gcc caa gct gtc gag ctg acc ttc gct ggg 1459

Asp Ala Val Glu Ala Ala Ala Gln Ala Val Glu Leu Thr Phe Ala Gly

440 445 450

gcg ttg ttc taagttttct agataacaag gccagcacag 1498

Ala Leu Phe

455

<210>130

<211>456

<212>PRT

<213>谷氨酸棒状杆菌

<400>130

Leu Ala Leu Lys Gly Tyr Thr Asn Phe Asp Gly Glu Phe Ile Glu Phe

1 5 10 15

Gly Ser Val Gln Ala Lys Glu Glu Glu Lys Arg Ala Phe Asp Asn Asp

20 25 30

Arg Ala His Val Phe His Ser Trp Ser Ala Gln Asp Lys Ile Ser Pro

35 40 45

Lys Val Trp Ala Ala Ala Glu Gly Ser Thr Leu Tyr Asp Phe Asp Gly

50 55 60

Asn Ala Phe Ile Asp Met Gly Ser Gln Leu Val Ser Ala Asn Leu Gly

65 70 75 80

His Asn Asn Pro Arg Leu Val Glu Ala Ile Gln Arg Gln Ala Ala Arg

85 90 95

Leu Thr Asn Ile Asn Pro Ala Phe Gly Asn Asp Val Arg Ser Asp Val

100 105 110

Ala Ala Lys Ile Val Ser Met Ala Arg Gly Glu Phe Ser His Val Phe

115 120 125

Phe Thr Asn Gly Gly Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala

130 135 140

Arg Leu His Thr Gly Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr

145 150 155 160

His Gly Ala Thr Gly Ser Ala Met Met Leu Thr Gly Glu His Arg Arg

165 170 175

Leu Gly Asn Pro Thr Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro

180 185 190

Phe Leu His His Ser Ser Phe Phe Ala Thr Thr Gln Glu Glu Glu Cys

195 200 205

Glu Arg Ala Leu Lys His Leu Glu Asp Val Ile Ala Phe Glu Gly Ala

210 215 220

Gly Met Ile Ala Ala Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly

225 230 235 240

Ile Ile Leu Pro Pro Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys

245 250 255

Asn Lys His Gly Ile Leu Phe Ile Ala Asp Glu Val Met Val Gly Phe

260 265 270

Gly Arg Thr Gly Lys Leu Phe Ala Tyr Glu His Ala Gly Asp Asp Phe

275 280 285

Gln Pro Asp Met Ile Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Ala

290 295 300

Pro Leu Gly Gly Ile Val Met Thr Gln Ser Ile Arg Asp Thr Phe Gly

305 310 315 320

Ser Glu Ala Tyr Ser Gly Gly Leu Thr Tyr Ser Gly His Pro Leu Ala

325 330 335

Val Ala Pro Ala Lys Ala Ala Leu Glu Ile Tyr Ala Glu Gly Glu Ile

340 345 350

Ile Pro Arg Val Ala Arg Leu Gly Ala Glu Leu Ile Glu Pro Arg Leu

355 360 365

Arg Glu Leu Ala Glu Glu Asn Val Ala Ile Ala Asp Val Arg Gly Ile

370 375 380

Gly Phe Phe Trp Ala Val Glu Phe Asn Ala Asp Ala Thr Ala Met Ala

385 390 395 400

Ala Gly Ala Ala Glu Phe Lys Glu Arg Gly Val Trp Pro Met Ile Ser

405 410 415

Gly Asn Arg Phe His Ile Ala Pro Pro Leu Thr Thr Thr Asp Asp Glu

420 425 430

Leu Val Ala Leu Leu Asp Ala Val Glu Ala Ala Ala Gln Ala Val Glu

435 440 445

Leu Thr Phe Ala Gly Ala Leu Phe

450 455

<210>131

<211>1231

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1201)

<223>RXA03003

<400>131

tcgatgatct gggctcccca ggtggtgcgg ctaagcttgg accacaagat tttgatcacc 60

caatgatcgc tgcgctgccg cctcaggcat aatctaacgc atg acc tct cgc acc 115

Met Thr Ser Arg Thr

1 5

ccg ctt gtt tct gtt ctt cct gat ttt ccg tgg gat tcg ctc gct tcc 163

Pro Leu Val Ser Val Leu Pro Asp Phe Pro Trp Asp Ser Leu Ala Ser

10 15 20

gca aaa gcc aaa gct gcg tct cac ccg gat ggg atc gtg aat ctt tct 211

Ala Lys Ala Lys Ala Ala Ser His Pro Asp Gly Ile Val Asn Leu Ser

25 30 35

gtt ggc act ccg gtt gat ccg gtc gcg ccc agc att cag atc gcg ttg 259

Val Gly Thr Pro Val Asp Pro Val Ala Pro Ser Ile Gln Ile Ala Leu

40 45 50

gca gaa gca gcg ggg ttt tcg ggt tac cct caa acc atc ggc acc ccg 307

Ala Glu Ala Ala Gly Phe Ser Gly Tyr Pro Gln Thr Ile Gly Thr Pro

55 60 65

gaa ctc cgc gca gcc atc agg ggc gcg ctt gag cgg cgc tac aac atg 355

Glu Leu Arg Ala Ala Ile Arg Gly Ala Leu Glu Arg Arg Tyr Asn Met

70 75 80 85

aca aag ctt gtc gac gcc tcc ctc ctc ccc gtc gtg ggt acc aag gag 403

Thr Lys Leu Val Asp Ala Ser Leu Leu Pro Val Val Gly Thr Lys Glu

90 95 100

gca att gcc ctt ctt cca ttc gcg ttg ggt att tcc ggc acc gtt gtc 45l

Ala Ile Ala Leu Leu Pro Phe Ala Leu Gly Ile Ser Gly Thr Val Val

105 110 115

atc cca gag att gcg tac cca acc tac gaa gtc gct gtc gtg gcc gca 499

Ile Pro Glu Ile Ala Tyr Pro Thr Tyr Glu Val Ala Val Val Ala Ala

120 125 130

gga tgc acc gtg ttg cgt tct gat tcg ctg ttt aag ctc ggc ccg cag 547

Gly Cys Thr Val Leu Arg Ser Asp Ser Leu Phe Lys Leu Gly Pro Gln

135 140 145

atc ccg tcg atg atg ttt atc aac tca cca tcc aac ccc aca ggc aag 595

Ile Pro Ser Met Met Phe Ile Asn Ser Pro Ser Asn Pro Thr Gly Lys

150 155 160 165

gtt ctg ggc atc cca cac ttg cgc aag gtt gtg aag tgg gcg cag gaa 643

Val Leu Gly Ile Pro His Leu Arg Lys Val Val Lys Trp Ala Gln Glu

170 175 180

aac aac gtg atc ctc gca gct gat gaa tgc tac ttg ggt ctt ggc tgg 691

Asn Asn Val Ile Leu Ala Ala Asp Glu Cys Tyr Leu Gly Leu Gly Trp

185 190 195

gac gat gaa aac cca ccg atc tca att ttg gat cca cgt gtc tgc gat 739

Asp Asp Glu Asn Pro Pro Ile Ser Ile Leu Asp Pro Arg Val Cys Asp

200 205 210

ggc gac cac acc aac ttg atc gcc att cac tcg ctg tct aaa acc tca 787

Gly Asp His Thr Asn Leu Ile Ala Ile His Ser Leu Ser Lys Thr Ser

215 220 225

aac ctc gct tct tac cgc gca ggt tac ctc gtt ggc gat cca gcg ctg 835

Asn Leu Ala Ser Tyr Arg Ala Gly Tyr Leu Val Gly Asp Pro Ala Leu

230 235 240 245

att ggt gaa ctc acg gaa gtc cgt aag aac ttg ggt ctc atg gtt cct 883

Ile Gly Glu Leu Thr Glu Val Arg Lys Asn Leu Gly Leu Met Val Pro

250 255 260

ttc cca atc cag cag gcc atg atc gca gcc ctc aac gac gat gac caa 931

Phe Pro Ile Gln Gln Ala Met Ile Ala Ala Leu Asn Asp Asp Asp Gln

265 270 275

gag gca ggg cag aag ctc acc tac gcg att cgt cga gca aaa ctc atg 979

Glu Ala Gly Gln Lys Leu Thr Tyr Ala Ile Arg Arg Ala Lys Leu Met

280 285 290

cgc gcc ctg ttg gaa tcc ggc ttt cag gta gat aat tct gaa gcg ggt 1027

Arg Ala Leu Leu Glu Ser Gly Phe Gln Val Asp Asn Ser Glu Ala Gly

295 300 305

ctg tac ctc tgg gcg acg cgt gaa gaa cct tgc cgt gac act gtc gat 1075

Leu Tyr Leu Trp Ala Thr Arg Glu Glu Pro Cys Arg Asp Thr Val Asp

310 315 320 325

tgg ttc gct gag cgt ggc att ctc gtt gcc cca gga gac ttc tat ggc 1123

Trp Phe Ala Glu Arg Gly Ile Leu Val Ala Pro Gly Asp Phe Tyr Gly

330 335 340

cct cgc gga gcg cag cat gtg cgt gtg gcg atg acc gaa acc gac gag 1171

Pro Arg Gly Ala Gln His Val Arg Val Ala Met Thr Glu Thr Asp Glu

345 350 355

cgc gtc gac gcc ttt gtt tct cgc ctg agc taaacacgac taagctttat 1221

Arg Val Asp Ala Phe Val Ser Arg Leu Ser

360 365

ttgtttaatt 1231

<210>132

<211>367

<212>PRT

<213>谷氨酸棒状杆菌

<400>132

Met Thr Ser Arg Thr Pro Leu Val Ser Val Leu Pro Asp Phe Pro Trp

1 5 10 15

Asp Ser Leu Ala Ser Ala Lys Ala Lys Ala Ala Ser His Pro Asp Gly

20 25 30

Ile Val Asn Leu Ser Val Gly Thr Pro Val Asp Pro Val Ala Pro Ser

35 40 45

Ile Gln Ile Ala Leu Ala Glu Ala Ala Gly Phe Ser Gly Tyr Pro Gln

50 55 60

Thr Ile Gly Thr Pro Glu Leu Arg Ala Ala Ile Arg Gly Ala Leu Glu

65 70 75 80

Arg Arg Tyr Asn Met Thr Lys Leu Val Asp Ala Ser Leu Leu Pro Val

85 90 95

Val Gly Thr Lys Glu Ala Ile Ala Leu Leu Pro Phe Ala Leu Gly Ile

100 105 110

Ser Gly Thr Val Val Ile Pro Glu Ile Ala Tyr Pro Thr Tyr Glu Val

115 120 125

Ala Val Val Ala Ala Gly Cys Thr Val Leu Arg Ser Asp Ser Leu Phe

130 135 140

Lys Leu Gly Pro Gln Ile Pro Ser Met Met Phe Ile Asn Ser Pro Ser

145 150 155 160

Asn Pro Thr Gly Lys Val Leu Gly Ile Pro His Leu Arg Lys Val Val

165 170 175

Lys Trp Ala Gln Glu Asn Asn Val Ile Leu Ala Ala Asp Glu Cys Tyr

180 185 190

Leu Gly Leu Gly Trp Asp Asp Glu Asn Pro Pro Ile Ser Ile Leu Asp

195 200 205

Pro Arg Val Cys Asp Gly Asp His Thr Asn Leu Ile Ala Ile His Ser

210 215 220

Leu Ser Lys Thr Ser Asn Leu Ala Ser Tyr Arg Ala Gly Tyr Leu Val

225 230 235 240

Sly Asp Pro Ala Leu Ile Gly Glu Leu Thr Glu Val Arg Lys Asn Leu

245 250 255

Gly Leu Met Val Pro Phe Pro Ile Gln Gln Ala Met Ile Ala Ala Leu

260 265 270

Asn Asp Asp Asp Gln Glu Ala Gly Gln Lys Leu Thr Tyr Ala Ile Arg

275 280 285

Arg Ala Lys Leu Met Arg Ala Leu Leu Glu Ser Gly Phe Gln Val Asp

290 295 300

Asn Ser Glu Ala Gly Leu Tyr Leu Trp Ala Thr Arg Glu Glu Pro Cys

305 310 315 320

Arg Asp Thr Val Asp Trp Phe Ala Glu Arg Gly Ile Leu Val Ala Pro

325 330 335

Gly Asp Phe Tyr Gly Pro Arg Gly Ala Gln His Val Arg Val Ala Met

340 345 350

Thr Glu Thr Asp Glu Arg Val Asp Ala Phe Val Ser Arg Leu Ser

355 360 365

<210>133

<211>763

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(733)

<223>RXA03171

<400>133

atactttgtt ggactggaaa agtggccgtt tggttccctc caagcccaaa ttcgcccgcg 60

cggtcttctt ctgggcggca atgatttaac atgtgaagct atg gac atc acc atc 115

Met Asp Ile Thr Ile

1 5

gtc aac cac cca ctc gtt gct agc cgc cta acc ctg ttg cgc gac gag 163

Val Asn His Pro Leu Val Ala Ser Arg Leu Thr Leu Leu Arg Asp Glu

10 15 20

cgc agc gac aac gca gct ttc cgt gca gca gcc aac gac ctc ggc gcc 211

Arg Ser Asp Asn Ala Ala Phe Arg Ala Ala Ala Asn Asp Leu Gly Ala

25 30 35

atg ctg atc tac gaa gca tcc cga gat ctg gaa gtc gaa cac ttc gac 259

Met Leu Ile Tyr Glu Ala Ser Arg Asp Leu Glu Val Glu His Phe Asp

40 45 50

acc aaa acc ccc gtt gcc atg gct gaa ggt act cgc ctg aag cag cca 307

Thr Lys Thr Pro Val Ala Met Ala Glu Gly Thr Arg Leu Lys Gln Pro

55 60 65

ccc atc atc gtt ccc atc atc cgt gca ggt ctc ggc atg atc gac cca 355

Pro Ile Ile Val Pro Ile Ile Arg Ala Gly Leu Gly Met Ile Asp Pro

70 75 80 85

gcg ctg tcg atg att ccg gat gca cag gtc ggc ttc att ggc ctt gcc 403

Ala Leu Ser Met Ile Pro Asp Ala Gln Val Gly Phe Ile Gly Leu Ala

90 95 100

cgc gat gag gaa acc cat gag cca gtc cca tac ctt gag gcg ctg cca 451

Arg Asp Glu Glu Thr His Glu Pro Val Pro Tyr Leu Glu Ala Leu Pro

105 110 115

cag gat cta agc aac cag cct gta ttc ctt gtc gat ccc atg ctg gcc 499

Gln Asp Leu Ser Asn Gln Pro Val Phe Leu Val Asp Pro Met Leu Ala

120 125 130

acc ggc ggt tcc ctc ctg cac gcg atc cgc ctt ctt gct gat cgt ggc 547

Thr Gly Gly Ser Leu Leu His Ala Ile Arg Leu Leu Ala Asp Arg Gly

135 140 145

gcc acc gac atc acc gcc atc tgc atg gtt tct gcg cag cca ggt gtg 595

Ala Thr Asp Ile Thr Ala Ile Cys Met Val Ser Ala Gln Pro Gly Val

150 155 160 165

gac gca ttg gcg gaa tct ggt ctt cca gtt cgt ttg gtt acc gcc acc 643

Asp Ala Leu Ala Glu Ser Gly Leu Pro Val Arg Leu Val Thr Ala Thr

170 175 180

atc gac cca ggt cta gac gaa aac gcc tac atc gtg cct ggt ctt gga 691

Ile Asp Pro Gly Leu Asp Glu Asn Ala Tyr Ile Val Pro Gly Leu Gly

185 190 195

gat gcc ggt gat cgt ctc tac ggt ccg cga aac atc gac ctt 733

Asp Ala Gly Asp Arg Leu Tyr Gly Pro Arg Asn Ile Asp Leu

200 205 210

taaaagtttc taagggcatt acggaaaatt 763

<210>134

<211>211

<212>PRT

<213>谷氨酸棒状杆菌

<400>134

Met Asp Ile Thr Ile Val Asn His Pro Leu Val Ala Ser Arg Leu Thr

1 5 10 15

Leu Leu Arg Asp Glu Arg Ser Asp Asn Ala Ala Phe Arg Ala Ala Ala

20 25 30

Asn Asp Leu Gly Ala Met Leu Ile Tyr Glu Ala Ser Arg Asp Leu Glu

35 40 45

Val Glu His Phe Asp Thr Lys Thr Pro Val Ala Met Ala Glu Gly Thr

50 55 60

Arg Leu Lys Gln Pro Pro Ile Ile Val Pro Ile Ile Arg Ala Gly Leu

65 70 75 80

Gly Met Ile Asp Pro Ala Leu Ser Met Ile Pro Asp Ala Gln Val Gly

85 90 95

Phe Ile Gly Leu Ala Arg Asp Glu Glu Thr His Glu Pro Val Pro Tyr

100 105 110

Leu Glu Ala Leu Pro Gln Asp Leu Ser Asn Gln Pro Val Phe Leu Val

115 120 125

Asp Pro Met Leu Ala Thr Gly Gly Ser Leu Leu His Ala Ile Arg Leu

130 135 140

Leu Ala Asp Arg Gly Ala Thr Asp Ile Thr Ala Ile Cys Met Val Ser

145 150 155 160

Ala Gln Pro Gly Val Asp Ala Leu Ala Glu Ser Gly Leu Pro Val Arg

165 170 175

Leu Val Thr Ala Thr Ile Asp Pro Gly Leu Asp Glu Asn Ala Tyr Ile

180 185 190

Val Pro Gly Leu Gly Asp Ala Gly Asp Arg Leu Tyr Gly Pro Arg Asn

195 200 205

Ile Asp Leu

210

<210>135

<211>1288

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1258)

<223>RXA03282

<400>135

aggggctagt tttacacaaa agtggacagc ttggtctatc attgccagaa gaccggtcct 60

tttagggcca tagaattctg attacaggag ttgatctacc ttg tct ttt gac cca 115

Leu Ser Phe Asp Pro

1 5

aac acc cag ggt ttc tcc act gca tcg att cac gct ggg tat gag cca 163

Asn Thr Gln Gly Phe Ser Thr Ala Ser Ile His Ala Gly Tyr Glu Pro

10 15 20

gac gac tac tac ggt tcg att aac acc cca atc tat gcc tcc acc acc 211

Asp Asp Tyr Tyr Gly Ser Ile Asn Thr Pro Ile Tyr Ala Ser Thr Thr

25 30 35

ttc gcg cag aac gct cca aac gaa ctg cgc aaa ggc tac gag tac acc 259

Phe Ala Gln Asn Ala Pro Asn Glu Leu Arg Lys Gly Tyr Glu Tyr Thr

40 45 50

cgt gtg ggc aac ccc acc atc gtg gca tta gag cag acc gtc gca gca 307

Arg Val Gly Asn Pro Thr Ile Val Ala Leu Glu Gln Thr Val Ala Ala

55 60 65

ctc gaa ggc gca aag tat ggc cgc gca ttc tcc tcc ggc atg gct gca 355

Leu Glu Gly Ala Lys Tyr Gly Arg Ala Phe Ser Ser Gly Met Ala Ala

70 75 80 85

acc gac atc ctg ttc cgc atc atc ctc aag ccg ggc gat cac atc gtc 403

Thr Asp Ile Leu Phe Arg Ile Ile Leu Lys Pro Gly Asp His Ile Val

90 95 100

ctc ggc aac gat gct tac ggc gga acc tac cgc ctg atc gac acc gta 451

Leu Gly Asn Asp Ala Tyr Gly Gly Thr Tyr Arg Leu Ile Asp Thr Val

105 110 115

ttc acc gca tgg ggc gtc gaa tac acc gtt gtt gat acc tcc gtc gtg 499

Phe Thr Ala Trp Gly Val Glu Tyr Thr Val Val Asp Thr Ser Val Val

120 125 130

gaa gag gtc aag gca gcg atc aag gac aac acc aag ctg atc tgg gtg 547

Glu Glu Val Lys Ala Ala Ile Lys Asp Asn Thr Lys Leu Ile Trp Val

135 140 145

gaa acc cca acc aac cca gca ctt ggc atc acc gac atc gaa gca gta 595

Glu Thr Pro Thr Asn Pro Ala Leu Gly Ile Thr Asp Ile Glu Ala Val

150 155 160 165

gca aag ctc acc gaa ggc acc aac gcc aag ctg gtt gtt gac aac acc 643

Ala Lys Leu Thr Glu Gly Thr Asn Ala Lys Leu Val Val Asp Asn Thr

170 175 180

ttc gca tcc cca tac ctg cag cag cca cta aaa ctc ggc gca cac gca 691

Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Lys Leu Gly Ala His Ala

185 190 195

gtc ctg cac tcc acc acc aag tac atc gga gga cac tcc gac gtt gtt 739

Val Leu His Ser Thr Thr Lys Tyr Ile Gly Gly His Ser Asp Val Val

200 205 210

ggc ggc ctt gtg gtt acc aac gac cag gaa atg gac gaa gaa ctg ctg 787

Gly Gly Leu Val Val Thr Asn Asp Gln Glu Met Asp Glu Glu Leu Leu

215 220 225

ttc atg cag ggc ggc atc gga ccg atc cca tca gtt ttc gat gca tac 835

Phe Met Gln Gly Gly Ile Gly Pro Ile Pro Ser Val Phe Asp Ala Tyr

230 235 240 245

ctg acc gcc cgt ggc ctc aag acc ctt gca gtg cgc atg gat cgc cac 883

Leu Thr Ala Arg Gly Leu Lys Thr Leu Ala Val Arg Met Asp Arg His

250 255 260

tgc gac aac gca gaa aag atc gcg gaa ttc ctg gac tcc cgc cca gag 931

Cys Asp Asn Ala Glu Lys Ile Ala Glu Phe Leu Asp Ser Arg Pro Glu

265 270 275

gtc tcc acc gtg ctc tac cca ggt ctg aag aac cac cca ggc cac gaa 979

Val Ser Thr Val Leu Tyr Pro Gly Leu Lys Asn His Pro Gly His Glu

280 285 290

gtc gca gcg aag cag atg aag cgc ttc ggc ggc atg atc tcc gtc cgt 1027

Val Ala Ala Lys Gln Met Lys Arg Phe Gly Gly Met Ile Ser Val Arg

295 300 305

ttc gca ggc ggc gaa gaa gca gct aag aag ttc tgt acc tcc acc aaa 1075

Phe Ala Gly Gly Glu Glu Ala Ala Lys Lys Phe Cys Thr Ser Thr Lys

310 315 320 325

ctg atc tgt ctg gcc gag tcc ctc ggt ggc gtg gaa tcc ctc ctg gag 1123

Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val Glu Ser Leu Leu Glu

330 335 340

cac cca gca acc atg acc cac cag tca gct gcc ggc tct cag ctc gag 1171

His Pro Ala Thr Met Thr His Gln Ser Ala Ala Gly Ser Gln Leu Glu

345 350 355

gtt ccc cgc gac ctc gtg cgc atc tcc att ggt att gaa gac att gaa 1219

Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly Ile Glu Asp Ile Glu

360 365 370

gac ctg ctc gca gat gtc gag cag gcc ctc aat aac ctt tagaaactat 1268

Asp Leu Leu Ala Asp Val Glu Gln Ala Leu Asn Asn Leu

375 380 385

ttggcggcaa gcagcttttc 1288

<210>136

<211>386

<212>PRT

<213>谷氨酸棒状杆菌

<400>136

Leu Ser Phe Asp Pro Asn Thr Gln Gly Phe Ser Thr Ala Ser Ile His

1 5 10 15

Ala Gly Tyr Glu Pro Asp Asp Tyr Tyr Gly Ser Ile Asn Thr Pro Ile

20 25 30

Tyr Ala Ser Thr Thr Phe Ala Gln Asn Ala Pro Asn Glu Leu Arg Lys

35 40 45

Gly Tyr Glu Tyr Thr Arg Val Gly Asn Pro Thr Ile Val Ala Leu Glu

50 55 60

Gln Thr Val Ala Ala Leu Glu Gly Ala Lys Tyr Gly Arg Ala Phe Ser

65 70 75 80

Ser Gly Met Ala Ala Thr Asp Ile Leu Phe Arg Ile Ile Leu Lys Pro

85 90 95

Gly Asp His Ile Val Leu Gly Asn Asp Ala Tyr Gly Gly Thr Tyr Arg

100 105 110

Leu Ile Asp Thr Val Phe Thr Ala Trp Gly Val Glu Tyr Thr Val Val

115 120 125

Asp Thr Ser Val Val Glu Glu Val Lys Ala Ala Ile Lys Asp Asn Thr

130 135 140

Lys Leu Ile Trp Val Glu Thr Pro Thr Asn Pro Ala Leu Gly Ile Thr

145 150 155 160

Asp Ile Glu Ala Val Ala Lys Leu Thr Glu Gly Thr Asn Ala Lys Leu

165 170 175

Val Val Asp Asn Thr Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Lys

180 185 190

Leu Gly Ala His Ala Val Leu His Ser Thr Thr Lys Tyr lle Gly Gly

195 200 205

His Ser Asp Val Val Gly Gly Leu Val Val Thr Asn Asp Gln Glu Met

210 215 220

Asp Glu Glu Leu Leu Phe Met Gln Gly Gly Ile Gly Pro Ile Pro Ser

225 230 235 240

Val Phe Asp Ala Tyr Leu Thr Ala Arg Gly Leu Lys Thr Leu Ala Val

245 250 255

Arg Met Asp Arg His Cys Asp Asn Ala Glu Lys Ile Ala Glu Phe Leu

260 265 270

Asp Ser Arg Pro Glu Val Ser Thr Val Leu Tyr Pro Gly Leu Lys Asn

275 280 285

His Pro Gly His Glu Val Ala Ala Lys Gln Met Lys Arg Phe Gly Gly

290 295 300

Met Ile Ser Val Arg Phe Ala Gly Gly Glu Glu Ala Ala Lys Lys Phe

305 310 315 320

Cys Thr Ser Thr Lys Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val

325 330 335

Glu Ser Leu Leu Glu His Pro Ala Thr Met Thr His Gln Ser Ala Ala

340 345 350

Gly Ser Gln Leu Glu Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly

355 360 365

Ile Glu Asp Ile Glu Asp Leu Leu Ala Asp Val Glu Gln Ala Leu Asn

370 375 380

Asn Leu

385

<210>137

<211>1336

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1306)

<223>RXA03465

<400>137

gatgaggtgg ataaaacctc cgtcggtaaa tatgacaaga aggacctccg caaccacctg 60

cgcaacggcg atttcgaagt aattaagctt aaagggccag gtg aaa aat aac ttc 115

Val Lys Asn Asn Phe

1 5

cct att tat tcc ggc ttt tac tgg ctt tat tcc gca gct ctt tct agg 163

Pro Ile Tyr Ser Gly Phe Tyr Trp Leu Tyr Ser Ala Ala Leu Ser Arg

10 15 20

atg ggc aag aag atg act act cgc ctt tat ctt tcg cca acg ccg ccc 211

Met Gly Lys Lys Met Thr Thr Arg Leu Tyr Leu Ser Pro Thr Pro Pro

25 30 35

cga aat gat cgg gag ggg gcg tcg aaa agc act tct gcg agc atc aaa 259

Arg Asn Asp Arg Glu Gly Ala Ser Lys Ser Thr Ser Ala Ser Ile Lys

40 45 50

cat gat gcg tac ctg cca ccc gca gac ggc aat cgc gtg ctt gtg gac 307

His Asp Ala Tyr Leu Pro Pro Ala Asp Gly Asn Arg Val Leu Val Asp

55 60 65

aga ttc gga cgc atc gcg cgt gac ctg cgg gtg tca ctg acc gac cga 355

Arg Phe Gly Arg Ile Ala Arg Asp Leu Arg Val Ser Leu Thr Asp Arg

70 75 80 85

tgc aac ctc cgc tgc acc tat tgc atg ccc gcg gag ggt tta gag tgg 403

Cys Asn Leu Arg Cys Thr Tyr Cys Met Pro Ala Glu Gly Leu Glu Trp

90 95 100

ctg ccc acc gag cag acg ctt aac gac gcc gag gtg ctg cga ctc atc 451

Leu Pro Thr Glu Gln Thr Leu Asn Asp Ala Glu Val Leu Arg Leu Ile

105 110 115

cgc att gcg gtg gtt aag ctg ggc att cgt caa att cga ttc acc ggc 499

Arg Ile Ala Val Val Lys Leu Gly Ile Arg Gln Ile Arg Phe Thr Gly

120 125 130

ggc gag cct tta ctg cgg aaa aat ttg gaa gac atc atc gcc ggc acc 547

Gly Glu Pro Leu Leu Arg Lys Asn Leu Glu Asp Ile Ile Ala Gly Thr

135 140 145

gca gcc ctg cgc acc gac gaa ggc gaa aaa gtt cac atc gct ctc acc 595

Ala Ala Leu Arg Thr Asp Glu Gly Glu Lys Val His Ile Ala Leu Thr

150 155 160 165

acc aac ggc ctt ggc cta gac aaa cgc atc gca gga ctg aaa gaa gct 643

Thr Asn Gly Leu Gly Leu Asp Lys Arg Ile Ala Gly Leu Lys Glu Ala

170 175 180

ggt ctt gac cgg gtc aat att tca ctc gac acc atc gac gcc gaa cgc 691

Gly Leu Asp Arg Val Asn Ile Ser Leu Asp Thr Ile Asp Ala Glu Arg

185 190 195

tac gtc tcg cta acc agg cgt gat cga ttg tcc ggt gtg ttg gcg tcc 739

Tyr Val Ser Leu Thr Arg Arg Asp Arg Leu Ser Gly Val Leu Ala Ser

200 205 210

atc gat gcc gct gtt gcc gct ggc ctt cac cca gtg aag atc aac gcc 787

Ile Asp Ala Ala Val Ala Ala Gly Leu His Pro Val Lys Ile Asn Ala

215 220 225

gtg gtc atg cct ggg gtc aat gaa gta gat atc gtc ccc ctt gcg gaa 835

Val Val Met Pro Gly Val Asn Glu Val Asp Ile Val Pro Leu Ala Glu

230 235 240 245

tac tgc att tcc aaa ggc tcc caa ctg cga ttc atc gaa caa atg cca 883

Tyr Cys Ile Ser Lys Gly Ser Gln Leu Arg Phe Ile Glu Gln Met Pro

250 255 260

ctt ggc ccg cgc gag cag tgg aaa cgc ggc gat atg gtc aca gcc gaa 931

Leu Gly Pro Arg Glu Gln Trp Lys Arg Gly Asp Met Val Thr Ala Glu

265 270 275

gaa atc ctg gcg cgc ctg gaa gaa aaa ttc acc tta tcc ccc gcc aag 979

Glu Ile Leu Ala Arg Leu Glu Glu Lys Phe Thr Leu Ser Pro Ala Lys

280 285 290

gaa ccc cga gga gct gca cct gct gcg ctg tgg aat gtg gta gat aaa 1027

Glu Pro Arg Gly Ala Ala Pro Ala Ala Leu Trp Asn Val Val Asp Lys

295 300 305

tcc aac cct gat atc act gga caa atc ggc atc atc gcc tcg gtg acg 1075

Ser Asn Pro Asp Ile Thr Gly Gln Ile Gly Ile Ile Ala Ser Val Thr

310 315 320 325

cac cca ttt tgc gga gat tgc gat cgc tcc cgc ctc acc acc gac ggc 1123

His Pro Phe Cys Gly Asp Cys Asp Arg Ser Arg Leu Thr Thr Asp Gly

330 335 340

acc atc cga aac tgc ctt ttc tcc cgc act gaa act ccc cta cgt gac 1171

Thr Ile Arg Asn Cys Leu Phe Ser Arg Thr Glu Thr Pro Leu Arg Asp

345 350 355

gcg ctt cgc gac ggc gcc tcc gac gat gag ctc gcg caa ctg tgg gca 1219

Ala Leu Arg Asp Gly Ala Ser Asp Asp Glu Leu Ala Gln Leu Trp Ala

360 365 370

ggc gcc atg tgg gag aag aaa ccc ggc cat ggc atc gac gat gaa ggc 1267

Gly Ala Met Trp Glu Lys Lys Pro Gly His Gly Ile Asp Asp Glu Gly

375 380 385

ttc ctc caa cca gat cgc ccc atg tct gcc atc ggt ggc tagcccatac 1316

Phe Leu Gln Pro Asp Arg Pro Met Ser Ala Ile Gly Gly

390 395 400

cagccccaac cagacaggcg 1336

<210>138

<211>402

<212>PRT

<213>谷氨酸棒状杆菌

<400>138

Val Lys Asn Asn Phe Pro Ile Tyr Ser Gly Phe Tyr Trp Leu Tyr Ser

1 5 10 15

Ala Ala Leu Ser Arg Met Gly Lys Lys Met Thr Thr Arg Leu Tyr Leu

20 25 30

Ser Pro Thr Pro Pro Arg Asn Asp Arg Glu Gly Ala Ser Lys Ser Thr

35 40 45

Ser Ala Ser Ile Lys His Asp Ala Tyr Leu Pro Pro Ala Asp Gly Asn

50 55 60

Arg Val Leu Val Asp Arg Phe Gly Arg Ile Ala Arg Asp Leu Arg Val

65 70 75 80

Ser Leu Thr Asp Arg Cys Asn Leu Arg Cys Thr Tyr Cys Met Pro Ala

85 90 95

Glu Gly Leu Glu Trp Leu Pro Thr Glu Gln Thr Leu Asn Asp Ala Glu

100 105 110

Val Leu Arg Leu Ile Arg Ile Ala Val Val Lys Leu Gly Ile Arg Gln

115 120 125

Ile Arg Phe Thr Gly Gly Glu Pro Leu Leu Arg Lys Asn Leu Glu Asp

130 135 140

Ile Ile Ala Gly Thr Ala Ala Leu Arg Thr Asp Glu Gly Glu Lys Val

145 150 155 160

His Ile Ala Leu Thr Thr Asn Gly Leu Gly Leu Asp Lys Arg Ile Ala

165 170 175

Gly Leu Lys Glu Ala Gly Leu Asp Arg Val Asn Ile Ser Leu Asp Thr

180 185 190

Ile Asp Ala Glu Arg Tyr Val Ser Leu Thr Arg Arg Asp Arg Leu Ser

195 200 205

Gly Val Leu Ala Ser Ile Asp Ala Ala Val Ala Ala Gly Leu His Pro

210 215 220

Val Lys Ile Asn Ala Val Val Met Pro Gly Val Asn Glu Val Asp Ile

225 230 235 240

Val Pro Leu Ala Glu Tyr Cys Ile Ser Lys Gly Ser Gln Leu Arg Phe

245 250 255

Ile Glu Gln Met Pro Leu Gly Pro Arg Glu Gln Trp Lys Arg Gly Asp

260 265 270

Met Val Thr Ala Glu Glu Ile Leu Ala Arg Leu Glu Glu Lys Phe Thr

275 280 285

Leu Ser Pro Ala Lys Glu Pro Arg Gly Ala Ala Pro Ala Ala Leu Trp

290 295 300

Asn Val Val Asp Lys Ser Asn Pro Asp Ile Thr Gly Gln Ile Gly Ile

305 310 315 320

Ile Ala Ser Val Thr His Pro Phe Cys Gly Asp Cys Asp Arg Ser Arg

325 330 335

Leu Thr Thr Asp Gly Thr Ile Arg Asn Cys Leu Phe Ser Arg Thr Glu

340 345 350

Thr Pro Leu Arg Asp Ala Leu Arg Asp Gly Ala Ser Asp Asp Glu Leu

355 360 365

Ala Gln Leu Trp Ala Gly Ala Met Trp Glu Lys Lys Pro Gly His Gly

370 375 380

Ile Asp Asp Glu Gly Phe Leu Gln Pro Asp Arg Pro Met Ser Ala Ile

385 390 395 400

Gly Gly

<210>139

<211>1402

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1372)

<223>RXA03677

<400>139

atttttgacc ctccgggggt gatttaacct aaaattccac acaaacgtgt tcgaggtcat 60

tagattgata agcatctgtt gttaagaaag gtgacttcct atg tcc tcg att tcc 115

Met Ser Ser Ile Ser

1 5

cgc aag acc ggc gcg tca ctt gca gcc acc aca ctg ttg gca gcg atc 163

Arg Lys Thr Gly Ala Ser Leu Ala Ala Thr Thr Leu Leu Ala Ala Ile

10 15 20

gca ctg gcc ggt tgt agt tca gac tca agc tcc gac tcc aca gat tcc 211

Ala Leu Ala Gly Cys Ser Ser Asp Ser Ser Ser Asp Ser Thr Asp Ser

25 30 35

acc gct agc gaa ggc gca gac agc cgc ggc ccc atc acc ttt gcg atg 259

Thr Ala Ser Glu Gly Ala Asp Ser Arg Gly Pro Ile Thr Phe Ala Met

40 45 50

ggc aaa aac gac acc gac aaa gtc att ccg atc atc gac cgc tgg aac 307

Gly Lys Asn Asp Thr Asp Lys Val Ile Pro Ile Ile Asp Arg Trp Asn

55 60 65

gaa gcc cac ccc gat gag cag gta acg ctc aac gaa ctc gcc ggt gaa 355

Glu Ala His Pro Asp Glu Gln Val Thr Leu Asn Glu Leu Ala Gly Glu

70 75 80 85

gcc gac gcg cag cgc gaa acc ctc gtg caa tcc ctg cag gcc ggc aac 403

Ala Asp Ala Gln Arg Glu Thr Leu Val Gln Ser Leu Gln Ala Gly Asn

90 95 100

tct gac tac gac gtc atg gcg ctc gac gtc atc tgg acc gca gac ttc 451

Ser Asp Tyr Asp Val Met Ala Leu Asp Val Ile Trp Thr Ala Asp Phe

105 110 115

gcg gca aac caa tgg ctc gca cca ctt gaa ggc gac ctc gag gta gac 499

Ala Ala Asn Gln Trp Leu Ala Pro Leu Glu Gly Asp Leu Glu Val Asp

120 125 130

acc tcc gga ctg ctg caa tcc acc gtg gat tcc gca acc tac aac ggc 547

Thr Ser Gly Leu Leu Gln Ser Thr Val Asp Ser Ala Thr Tyr Asn Gly

135 140 145

acc ctc tac gca ctg cca cag aac acc aac ggc cag cta ctg ttc cgc 595

Thr Leu Tyr Ala Leu Pro Gln Asn Thr Asn Gly Gln Leu Leu Phe Arg

150 155 160 165

aac acc gaa atc atc cca gaa gca cca gca aac tgg gct gac ctc gtg 643

Asn Thr Glu Ile Ile Pro Glu Ala Pro Ala Asn Trp Ala Asp Leu Val

170 175 180

gaa tcc tgc acg ctt gct gaa gaa gca ggc gtt gat tgc ctg acc act 691

Glu Ser Cys Thr Leu Ala Glu Glu Ala Gly Val Asp Cys Leu Thr Thr

185 190 195

cag ctc aag cag tac gaa ggc ctt tca gtg aac acc atc ggc ttc atc 739

Gln Leu Lys Gln Tyr Glu Gly Leu Ser Val Asn Thr Ile Gly Phe Ile

200 205 210

gaa ggt tgg gga ggc agc gtc cta gac gat gac ggc aac gtc acc gta 787

Glu Gly Trp Gly Gly Ser Val Leu Asp Asp Asp Gly Asn Val Thr Val

215 220 225

gac agc gac gac gcc aag gca ggc ctt caa gcg ctt gtc gac ggc ttc 835

Asp Ser Asp Asp Ala Lys Ala Gly Leu Gln Ala Leu Val Asp Gly Phe

230 235 240 245

gac gac ggc acc atc tcc aag gca tcc ctt gca gcg acc gaa gaa gaa 883

Asp Asp Gly Thr Ile Ser Lys Ala Ser Leu Ala Ala Thr Glu Glu Glu

250 255 260

acc aac ctc gca ttc acc gaa ggc caa acc gcc tac gcc att aac tgg 931

Thr Asn Leu Ala Phe Thr Glu Gly Gln Thr Ala Tyr Ala Ile Asn Trp

265 270 275

cca tac atg tac acc aac tcc gaa gaa gcc gaa gca acc gca ggc aaa 979

Pro Tyr Met Tyr Thr Asn Ser Glu Glu Ala Glu Ala Thr Ala Gly Lys

280 285 290

ttc gaa gta cag ccc ctc gta ggt aaa gac ggc gtc ggc gta tcc acc 1027

Phe Glu Val Gln Pro Leu Val Gly Lys Asp Gly Val Gly Val Ser Thr

295 300 305

ctt ggt ggc tac aac aac ggc atc aac gtc aac tcc gaa aac aag gca 1075

Leu Gly Gly Tyr Asn Asn Gly Ile Asn Val Asn Ser Glu Asn Lys Ala

310 315 320 325

acc gcc cgc gac ttc atc gaa ttc atc atc aac gaa gag aac caa acc 1123

Thr Ala Arg Asp Phe Ile Glu Phe Ile Ile Asn Glu Glu Asn Gln Thr

330 335 340

tgg ttc gcg gac aac tcc ttc cca cca gtt ctg gca tcc atc tac gat 1171

Trp Phe Ala Asp Asn Ser Phe Pro Pro Val Leu Ala Ser Ile Tyr Asp

345 350 355

gat gag tcc ctt gtt gag cag tac cca tac ctg cca gca ctg aag gaa 1219

Asp Glu Ser Leu Val Glu Gln Tyr Pro Tyr Leu Pro Ala Leu Lys Glu

360 365 370

tcc ctg gaa aac gca gca cca cgc cca gtg tct cct ttc tac cca gcc 1267

Ser Leu Glu Asn Ala Ala Pro Arg Pro Val Ser Pro Phe Tyr Pro Ala

375 380 385

atc tcc aag gca atc cag gac aac gcc tac gca gcg ctt aac ggc aac 1315

Ile Ser Lys Ala Ile Gln Asp Asn Ala Tyr Ala Ala Leu Asn Gly Asn

390 395 400 405

gtc gac gtt gac cag gca acc acc gat atg aag gca gcg atc gaa aac 1363

Val Asp Val Asp Gln Ala Thr Thr Asp Met Lys Ala Ala Ile Glu Asn

410 415 420

gct tcc agc tagttcggta atttagttca ttctccggcc 1402

Ala Ser Ser

<210>140

<211>424

<212>PRT

<213>谷氨酸棒状杆菌

<400>140

Met Ser Ser Ile Ser Arg Lys Thr Gly Ala Ser Leu Ala Ala Thr Thr

1 5 10 15

Leu Leu Ala Ala Ile Ala Leu Ala Gly Cys Ser Ser Asp Ser Ser Ser

20 25 30

Asp Ser Thr Asp Ser Thr Ala Ser Glu Gly Ala Asp Ser Arg Gly Pro

35 40 45

Ile Thr Phe Ala Met Gly Lys Asn Asp Thr Asp Lys Val Ile Pro Ile

50 55 60

Ile Asp Arg Trp Asn Glu Ala His Pro Asp Glu Gln Val Thr Leu Asn

65 70 75 80

Glu Leu Ala Gly Glu Ala Asp Ala Gln Arg Glu Thr Leu Val Gln Ser

85 90 95

Leu Gln Ala Gly Asn Ser Asp Tyr Asp Val Met Ala Leu Asp Val Ile

100 105 110

Trp Thr Ala Asp Phe Ala Ala Asn Gln Trp Leu Ala Pro Leu Glu Gly

115 120 125

Asp Leu Glu Val Asp Thr Ser Gly Leu Leu Gln Ser Thr Val Asp Ser

130 135 140

Ala Thr Tyr Asn Gly Thr Leu Tyr Ala Leu Pro Gln Asn Thr Asn Gly

145 150 155 160

Gln Leu Leu Phe Arg Asn Thr Glu Ile Ile Pro Glu Ala Pro Ala Asn

165 170 175

Trp Ala Asp Leu Val Glu Ser Cys Thr Leu Ala Glu Glu Ala Gly Val

180 185 190

Asp Cys Leu Thr Thr Gln Leu Lys Gln Tyr Glu Gly Leu Ser Val Asn

195 200 205

Thr Ile Gly Phe Ile Glu Gly Trp Gly Gly Ser Val Leu Asp Asp Asp

210 215 220

Gly Asn Val Thr Val Asp Ser Asp Asp Ala Lys Ala Gly Leu Gln Ala

225 230 235 240

Leu Val Asp Gly Phe Asp Asp Gly Thr Ile Ser Lys Ala Ser Leu Ala

245 250 255

Ala Thr Glu Glu Glu Thr Asn Leu Ala Phe Thr Glu Gly Gln Thr Ala

260 265 270

Tyr Ala Ile Asn Trp Pro Tyr Met Tyr Thr Asn Ser Glu Glu Ala Glu

275 280 285

Ala Thr Ala Gly Lys Phe Glu Val Gln Pro Leu Val Gly Lys Asp Gly

290 295 300

Val Gly Val Ser Thr Leu Gly Gly Tyr Asn Asn Gly Ile Asn Val Asn

305 310 315 320

Ser Glu Asn Lys Ala Thr Ala Arg Asp Phe Ile Glu Phe Ile Ile Asn

325 330 335

Glu Glu Asn Gln Thr Trp Phe Ala Asp Asn Ser Phe Pro Pro Val Leu

340 345 350

Ala Ser Ile Tyr Asp Asp Glu Ser Leu Val Glu Gln Tyr Pro Tyr Leu

355 360 365

Pro Ala Leu Lys Glu Ser Leu Glu Asn Ala Ala Pro Arg Pro Val Ser

370 375 380

Pro Phe Tyr Pro Ala Ile Ser Lys Ala Ile Gln Asp Asn Ala Tyr Ala

385 390 395 400

Ala Leu Asn Gly Asn Val Asp Val Asp Gln Ala Thr Thr Asp Met Lys

405 410 415

Ala Ala Ile Glu Asn Ala Ser Ser

420

<210>141

<211>901

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(871)

<223>RXA03695

<400>141

atagctacat acaatggaaa attacgtatg atgcgtctca cgctaacctg ataagaaagg 60

aaaaataagg gacaatcccc gcagaggggg tagccaagcg gtg tca gga gca gtg 115

Val Ser Gly Ala Val

1 5

ccc gag acg ccg tac cat caa cga tgg ctc gct ttc ccc gat gct gtg 163

Pro Glu Thr Pro Tyr His Gln Arg Trp Leu Ala Phe Pro Asp Ala Val

10 15 20

tcg tcc tac agg ccg tta gaa tcc aga ctc gcg ggc act agc cgc gcg 211

Ser Ser Tyr Arg Pro Leu Glu Ser Arg Leu Ala Gly Thr Ser Arg Ala

25 30 35

act gcc gac aac cgc acg ccg tgt acc gga agg ctt gga acg cct agg 259

Thr Ala Asp Asn Arg Thr Pro Cys Thr Gly Arg Leu Gly Thr Pro Arg

40 45 50

gtg ttc ctc atg gca aca gat gac gtt agc aag gcc tac agc tca ccg 307

Val Phe Leu Met Ala Thr Asp Asp Val Ser Lys Ala Tyr Ser Ser Pro

55 60 65

acc ttt gat gcg gag gca ctg ctc gga acg gta atc tcg gcc gag gac 355

Thr Phe Asp Ala Glu Ala Leu Leu Gly Thr Val Ile Ser Ala Glu Asp

70 75 80 85

ccc gac cgc gtg ctc atc gag ccc tgg gcg acc ggc gtc gac ggg gtg 403

Pro Asp Arg Val Leu Ile Glu Pro Trp Ala Thr Gly Val Asp Gly Val

90 95 100

atc ctc gat gtg ggc tct ggt acc ggg cgg tgg acc ggt cac ctc gcg 451

Ile Leu Asp Val Gly Ser Gly Thr Gly Arg Trp Thr Gly His Leu Ala

105 110 115

agc ctc gga cac cag atc gag gga ctg gaa cca gcg aca cgg ctc gta 499

Ser Leu Gly His Gln Ile Glu Gly Leu Glu Pro Ala Thr Arg Leu Val

120 125 130

gag ctc gct cgt cag aca cat ccc tca gtg acc ttt cac cac ggc act 547

Glu Leu Ala Arg Gln Thr His Pro Ser Val Thr Phe His His Gly Thr

135 140 145

att acc gac ctc tca gac tcg ccg aag cgc tgg gca ggc ctg ttg gcc 595

Ile Thr Asp Leu Ser Asp Ser Pro Lys Arg Trp Ala Gly Leu Leu Ala

150 155 160 165

tgg tac tcc ctg atc cac atg ggc ccg ggc gag ctg ccc gac gcc ctg 643

Trp Tyr Ser Leu Ile His Met Gly Pro Gly Glu Leu Pro Asp Ala Leu

170 175 180

gtc gca ctg cgg atg gcg gtg gag gat ggt ggt ggc ctg ctg atg tcc 691

Val Ala Leu Arg Met Ala Val Glu Asp Gly Gly Gly Leu Leu Met Ser

185 190 195

ttt ttc tcc ggg cct tct ctg gaa ccg atg tat cat ccc gtg gcc acc 739

Phe Phe Ser Gly Pro Ser Leu Glu Pro Met Tyr His Pro Val Ala Thr

200 205 210

gcc tac cgg tgg cca ctg ccc gag ctt gcc cag gca ctg gag acc gcg 787

Ala Tyr Arg Trp Pro Leu Pro Glu Leu Ala Gln Ala Leu Glu Thr Ala

215 220 225

ggt ttc cag gtc acc agt agt cac tgg gat cct cgg ttc ccc cat gcc 835

Gly Phe Gln Val Thr Ser Ser His Trp Asp Pro Arg Phe Pro His Ala

230 235 240 245

tat ctc acc gcc gag gcc agc aag cag aat ctc gcc tgacagtggt 881

Tyr Leu Thr Ala Glu Ala Ser Lys Gln Asn Leu Ala

250 255

ggcctactcg gtagtcgtct 901

<210>142

<211>257

<212>PRT

<213>谷氨酸棒状杆菌

<400>142

Val Ser Gly Ala Val Pro Glu Thr Pro Tyr His Gln Arg Trp Leu Ala

1 5 10 15

Phe Pro Asp Ala Val Ser Ser Tyr Arg Pro Leu Glu Ser Arg Leu Ala

20 25 30

Gly Thr Ser Arg Ala Thr Ala Asp Asn Arg Thr Pro Cys Thr Gly Arg

35 40 45

Leu Gly Thr Pro Arg Val Phe Leu Met Ala Thr Asp Asp Val Ser Lys

50 55 60

Ala Tyr Ser Ser Pro Thr Phe Asp Ala Glu Ala Leu Leu Gly Thr Val

65 70 75 80

Ile Ser Ala Glu Asp Pro Asp Arg Val Leu Ile Glu Pro Trp Ala Thr

85 90 95

Gly Val Asp Gly Val Ile Leu Asp Val Gly Ser Gly Thr Gly Arg Trp

100 105 110

Thr Gly His Leu Ala Ser Leu Gly His Gln Ile Glu Gly Leu Glu Pro

115 120 125

Ala Thr Arg Leu Val Glu Leu Ala Arg Gln Thr His Pro Ser Val Thr

130 135 140

Phe His His Gly Thr Ile Thr Asp Leu Ser Asp Ser Pro Lys Arg Trp

145 150 155 160

Ala Gly Leu Leu Ala Trp Tyr Ser Leu Ile His Met Gly Pro Gly Glu

165 170 175

Leu Pro Asp Ala Leu Val Ala Leu Arg Met Ala Val Glu Asp Gly Gly

180 185 190

Gly Leu Leu Met Ser Phe Phe Ser Gly Pro Ser Leu Glu Pro Met Tyr

195 200 205

His Pro Val Ala Thr Ala Tyr Arg Trp Pro Leu Pro Glu Leu Ala Gln

210 215 220

Ala Leu Glu Thr Ala Gly Phe Gln Val Thr Ser Ser His Trp Asp Pro

225 230 235 240

Arg Phe Pro His Ala Tyr Leu Thr Ala Glu Ala Ser Lys Gln Asn Leu

245 250 255

Ala

<210>143

<211>1582

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1552)

<223>RXA03754

<400>143

tttgggcggg aggtgacatt tatgcctctt aatagctata ctgtcccatg aaacgatatt 60

ttaatctcat atagtgagat ttacagattt ttaaaggacg gtg agt tcc atg acc 115

Val Ser Ser Met Thr

1 5

agc ccc gtg gag aac agc acc tca act gag aag ctg acc ctg gca gag 163

Ser Pro Val Glu Asn Ser Thr Ser Thr Glu Lys Leu Thr Leu Ala Glu

10 15 20

aag gtg tgg cgc gac cat gtc gtg tcc aag gga gaa aac ggc gag ccc 211

Lys Val Trp Arg Asp His Val Val Ser Lys Gly Glu Asn Gly Glu Pro

25 30 35

gac ctc ctc tac atc gac ctg cag ctg ctg cat gaa gtg acc tca cca 259

Asp Leu Leu Tyr Ile Asp Leu Gln Leu Leu His Glu Val Thr Ser Pro

40 45 50

cag gca ttt gac ggc ctg cgc atg acc ggc cgt aaa ctg cgc cac cca 307

Gln Ala Phe Asp Gly Leu Arg Met Thr Gly Arg Lys Leu Arg His Pro

55 60 65

gaa ctg cac ctg gcc acc gaa gac cac aac gtg cca acc gaa ggc atc 355

Glu Leu His Leu Ala Thr Glu Asp His Asn Val Pro Thr Glu Gly Ile

70 75 80 85

aag act ggc tca ctg ctg gaa atc aac gac aag att tcc cgc ctg cag 403

Lys Thr Gly Ser Leu Leu Glu Ile Asn Asp Lys Ile Ser Arg Leu Gln

90 95 100

gta tct act ctg cgc gac aac tgt gaa gaa ttc ggc gtg cgc ctg cac 451

Val Ser Thr Leu Arg Asp Asn Cys Glu Glu Phe Gly Val Arg Leu His

105 110 115

cca atg ggt gat gtc cga cag ggc atc gtg cac acc gtc ggc cca cag 499

Pro Met Gly Asp Val Arg Gln Gly Ile Val His Thr Val Gly Pro Gln

120 125 130

ctc ggc gca acc cag cca ggc atg acc att gtg tgc ggt gac tcc cac 547

Leu Gly Ala Thr Gln Pro Gly Met Thr Ile Val Cys Gly Asp Ser His

135 140 145

acc tcc acc cac ggt gct ttt ggc tcc atg gca ttc ggc atc ggt acc 595

Thr Ser Thr His Gly Ala Phe Gly Ser Met Ala Phe Gly Ile Gly Thr

150 155 160 165

tca gag gtt gag cac gtc atg gct act caa acc ctg cca ctg aag cct 643

Ser Glu Val Glu His Val Met Ala Thr Gln Thr Leu Pro Leu Lys Pro

170 175 180

ttc aag acc atg gcc att gaa gtt act ggt gaa ctg cag cca ggt gtt 691

Phe Lys Thr Met Ala Ile Glu Val Thr Gly Glu Leu Gln Pro Gly Val

185 190 195

tcc tcc aag gac ctg att ctg gcg att atc gcc aag atc ggc acc ggc 739

Ser Ser Lys Asp Leu Ile Leu Ala Ile Ile Ala Lys Ile Gly Thr Gly

200 205 210

ggc gga cag ggc tac gtt ctg gaa tac cgc ggc gaa gca atc cgt aag 787

Gly Gly Gln Gly Tyr Val Leu Glu Tyr Arg Gly Glu Ala Ile Arg Lys

215 220 225

atg tcc atg gat gca cgc atg acc atg tgc aac atg tcc atc gaa gct 835

Met Ser Met Asp Ala Arg Met Thr Met Cys Asn Met Ser Ile Glu Ala

230 235 240 245

ggc gca cgt gcc ggc atg atc gcc cca gac caa acc acc ttc gac tac 883

Gly Ala Arg Ala Gly Met Ile Ala Pro Asp Gln Thr Thr Phe Asp Tyr

250 255 260

gtt gaa ggc cgc gaa atg gca cca aag ggc gcc gac tgg gac gaa gca 931

Val Glu Gly Arg Glu Met Ala Pro Lys Gly Ala Asp Trp Asp Glu Ala

265 270 275

gtt gct tac tgg aag acc ctg cca acc gac gaa ggc gca acc ttt gac 979

Val Ala Tyr Trp Lys Thr Leu Pro Thr Asp Glu Gly Ala Thr Phe Asp

280 285 290

aag gtc gta gaa atc gat ggc tcg gca ctg acc cca ttc atc acc tgg 1027

Lys Val Val Glu Ile Asp Gly Ser Ala Leu Thr Pro Phe Ile Thr Trp

295 300 305

ggc acc aac cca ggc cag ggc ctg cca ctg ggc gaa tcc gta cca agc 1075

Gly Thr Asn Pro Gly Gln Gly Leu Pro Leu Gly Glu Ser Val Pro Ser

310 315 320 325

cca gaa gac ttc acc aac gac aac gac aag gca gca gcc gaa aag gca 1123

Pro Glu Asp Phe Thr Asn Asp Asn Asp Lys Ala Ala Ala Glu Lys Ala

330 335 340

ctg cag tac atg gac ctg gta cca gga acc cca ctg cgc gac atc aag 1171

Leu Gln Tyr Met Asp Leu Val Pro Gly Thr Pro Leu Arg Asp Ile Lys

345 350 355

atc gac acc gtc ttc ctg gga tcc tgc acc aac gcc cgc atc gaa gac 1219

Ile Asp Thr Val Phe Leu Gly Ser Cys Thr Asn Ala Arg Ile Glu Asp

360 365 370

ctg cag atc gcc gct gac atc ctc aag ggc cac aaa atc gcc gac ggc 1267

Leu Gln Ile Ala Ala Asp Ile Leu Lys Gly His Lys Ile Ala Asp Gly

375 380 385

atg cgc atg atg gtc gtg cct tcc tcc acc tgg atc aag caa gag gca 1315

Met Arg Met Met Val Val Pro Ser Ser Thr Trp Ile Lys Gln Glu Ala

390 395 400 405

gaa gcg ctc gga ctg gac aaa atc ttc acc gac gct ggc gct gaa tgg 1363

Glu Ala Leu Gly Leu Asp Lys Ile Phe Thr Asp Ala Gly Ala Glu Trp

410 415 420

cgt acc gca ggc tgc tcc atg tgc ctg ggc atg aac cca gac caa ctg 1411

Arg Thr Ala Gly Cys Ser Met Cys Leu Gly Met Asn Pro Asp Gln Leu

425 430 435

aag cca ggc gag cgc tcc gca tcc acc tcc aac cga aac ttc gaa gga 1459

Lys Pro Gly Glu Arg Ser Ala Ser Thr Ser Asn Arg Asn Phe Glu Gly

440 445 450

cgc caa gga cca gga ggc cgc acc cac ctg gta tcc cca gca gtc gca 1507

Arg Gln Gly Pro Gly Gly Arg Thr His Leu Val Ser Pro Ala Val Ala

455 460 465

gcc gcc acc gca atc cgc ggc acc ctg tcc tca cct gca gat atc 1552

Ala Ala Thr Ala Ile Arg Gly Thr Leu Ser Ser Pro Ala Asp Ile

470 475 480

taaggaaggc tagaaaacaa tggaaaaatt 1582

<210>144

<211>484

<212>PRT

<213>谷氨酸棒状杆菌

<400>144

Val Ser Ser Met Thr Ser Pro Val Glu Asn Ser Thr Ser Thr Glu Lys

1 5 10 15

Leu Thr Leu Ala Glu Lys Val Trp Arg Asp His Val Val Ser Lys Gly

20 25 30

Glu Asn Gly Glu Pro Asp Leu Leu Tyr Ile Asp Leu Gln Leu Leu His

35 40 45

Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Met Thr Gly Arg

50 55 60

Lys Leu Arg His Pro Glu Leu His Leu Ala Thr Glu Asp His Asn Val

65 70 75 80

Pro Thr Glu Gly Ile Lys Thr Gly Ser Leu Leu Glu Ile Asn Asp Lys

85 90 95

Ile Ser Arg Leu Gln Val Ser Thr Leu Arg Asp Asn Cys Glu Glu Phe

100 105 110

Gly Val Arg Leu His Pro Met Gly Asp Val Arg Gln Gly Ile Val His

115 120 125

Thr Val Gly Pro Gln Leu Gly Ala Thr Gln Pro Gly Met Thr Ile Val

130 135 140

Cys Gly Asp Ser His Thr Ser Thr His Gly Ala Phe Gly Ser Met Ala

145 150 155 160

Phe Gly Ile Gly Thr Ser Glu Val Glu His Val Met Ala Thr Gln Thr

165 170 175

Leu Pro Leu Lys Pro Phe Lys Thr Met Ala Ile Glu Val Thr Gly Glu

180 185 190

Leu Gln Pro Gly Val Ser Ser Lys Asp Leu Ile Leu Ala Ile Ile Ala

195 200 205

Lys Ile Gly Thr Gly Gly Gly Gln Gly Tyr Val Leu Glu Tyr Arg Gly

210 215 220

Glu Ala Ile Arg Lys Met Ser Met Asp Ala Arg Met Thr Met Cys Asn

225 230 235 240

Met Ser Ile Glu Ala Gly Ala Arg Ala Gly Met Ile Ala Pro Asp Gln

245 250 255

Thr Thr Phe Asp Tyr Val Glu Gly Arg Glu Met Ala Pro Lys Gly Ala

260 265 270

Asp Trp Asp Glu Ala Val Ala Tyr Trp Lys Thr Leu Pro Thr Asp Glu

275 280 285

Gly Ala Thr Phe Asp Lys Val Val Glu Ile Asp Gly Ser Ala Leu Thr

290 295 300

Pro Phe Ile Thr Trp Gly Thr Asn Pro Gly Gln GlyLeu Pro Leu Gly

305 310 315 320

Glu Ser Val Pro Ser Pro Glu Asp Phe Thr Asn Asp Asn Asp Lys Ala

325 330 335

Ala Ala Glu Lys Ala Leu Gln Tyr Met Asp Leu Val Pro Gly Thr Pro

340 345 350

Leu Arg Asp Ile Lys Ile Asp Thr Val Phe Leu Gly Ser Cys Thr Asn

355 360 365

Ala Arg Ile Glu Asp Leu Gln Ile Ala Ala Asp Ile Leu Lys Gly His

370 375 380

Lys Ile Ala Asp Gly Met Arg Met Met Val Val Pro Ser Ser Thr Trp

385 390 395 400

Ile Lys Gln Glu Ala Glu Ala Leu Gly Leu Asp Lys Ile Phe Thr Asp

405 410 415

Ala Gly Ala Glu Trp Arg Thr Ala Gly Cys Ser Met Cys Leu Gly Met

420 425 430

Asn Pro Asp Gln Leu Lys Pro Gly Glu Arg Ser Ala Ser Thr Ser Asn

435 440 445

Arg Asn Phe Glu Gly Arg Gln Gly Pro Gly Gly Arg Thr His Leu Val

450 455 460

Ser Pro Ala Val Ala Ala Ala Thr Ala Ile Arg Gly Thr Leu Ser Ser

465 470 475 480

Pro Ala Asp Ile

<210>145

<211>709

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(679)

<223>RXA03829

<400>145

cgaccttgtc ctagctctac gcaatgccca aaatcccctc cctcttgcag gtcgtggcac 60

acgcgaagca cagcaaagca tcctggaatt agcagatctt ttg gca gct gat gta 115

Leu Ala Ala Asp Val

1 5

gcc acc agc gcg ccc gcc cga gga ttc ttc cat ccc tca gga aac tct 163

Ala Thr Ser Ala Pro Ala Arg Gly Phe Phe His Pro Ser Gly Asn Ser

10 15 20

cgc gac ctg gtc gta tgt gga ggt ttc gct gcg acc ggt gca gct tca 211

Arg Asp Leu Val Val Cys Gly Gly Phe Ala Ala Thr Gly Ala Ala Ser

25 30 35

gaa ata cgc cga gcc aac gtg gcg ctt gtg ctg ggc gcc ggt ttg aac 259

Glu Ile Arg Arg Ala Asn Val Ala Leu Val Leu Gly Ala Gly Leu Asn

40 45 50

caa ttc acc atg gca ttc ggg gag gct ttc ggt gaa ctc gcg gaa gtg 307

Gln Phe Thr Met Ala Phe Gly Glu Ala Phe Gly Glu Leu Ala Glu Val

55 60 65

ctc caa gtg gat ctt gaa aca cag acc acc aac ccg cgc att aac cat 355

Leu Gln Val Asp Leu Glu Thr Gln Thr Thr Asn Pro Arg Ile Asn His

70 75 80 85

ttc att agc gca gat aac aca act gtt gta gcc gca gtg ctt tta aag 403

Phe Ile Ser Ala Asp Asn Thr Thr Val Val Ala Ala Val Leu Leu Lys

90 95 100

ctt cgt aca caa aat ttc cac gca ccg cgc cgg cta tac ctg gac gat 451

Leu Arg Thr Gln Asn Phe His Ala Pro Arg Arg Leu Tyr Leu Asp Asp

105 110 115

aat cct ctg aca tgc cct ggc ggc gac gcc ctt gca gcc gac ggc cga 499

Asn Pro Leu Thr Cys Pro Gly Gly Asp Ala Leu Ala Ala Asp Gly Arg

120 125 130

ctc gat cca cac agc ctc atg cgc caa ctt aac ggt att ttg cca gct 547

Leu Asp Pro His Ser Leu Met Arg Gln Leu Asn Gly Ile Leu Pro Ala

135 140 145

aac aag ttc gtc gcc tcc gat ggc gga cac ttc atc gga ggg gcc aac 595

Asn Lys Phe Val Ala Ser Asp Gly Gly His Phe Ile Gly Gly Ala Asn

150 155 160 165

acc tac ttc gac ctg gaa tca cga gac agc atc gtg ctt ttg gga acc 643

Thr Tyr Phe Asp Leu Glu Ser Arg Asp Ser Ile Val Leu Leu Gly Thr

170 175 180

gcc ttc aat cca tcg gcc tcg gct tcc cca ccg ccg taggtgtcgc 689

Ala Phe Asn Pro Ser Ala Ser Ala Ser Pro Pro Pro

185 190

cacagctgct gggcaagagt 709

<210>146

<211>193

<212>PRT

<213>谷氨酸棒状杆菌

<400>146

Leu Ala Ala Asp Val Ala Thr Ser Ala Pro Ala Arg Gly Phe Phe His

1 5 10 15

Pro Ser Gly Asn Ser Arg Asp Leu Val Val Cys Gly Gly Phe Ala Ala

20 25 30

Thr Gly Ala Ala Ser Glu Ile Arg Arg Ala Asn Val Ala Leu Val Leu

35 40 45

Gly Ala Gly Leu Asn Gln Phe Thr Met Ala Phe Gly Glu Ala Phe Gly

50 55 60

Glu Leu Ala Glu Val Leu Gln Val Asp Leu Glu Thr Gln Thr Thr Asn

65 70 75 80

Pro Arg Ile Asn His Phe Ile Ser Ala Asp Asn Thr Thr Val Val Ala

85 90 95

Ala Val Leu Leu Lys Leu Arg Thr Gln Asn Phe His Ala Pro Arg Arg

100 105 110

Leu Tyr Leu Asp Asp Asn Pro Leu Thr Cys Pro Gly Gly Asp Ala Leu

115 120 125

Ala Ala Asp Gly Arg Leu Asp Pro His Ser Leu Met Arg Gln Leu Asn

130 135 140

Gly Ile Leu Pro Ala Asn Lys Phe Val Ala Ser Asp Gly Gly His Phe

145 150 155 160

Ile Gly Gly Ala Asn Thr Tyr Phe Asp Leu Glu Ser Arg Asp Ser Ile

165 170 175

Val Leu Leu Gly Thr Ala Phe Asn Pro Ser Ala Ser Ala Ser Pro Pro

180 185 190

Pro

<210>147

<211>1315

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1285)

<223>RXA03871

<400>147

taaagccttg ggagcggtga ttatgctttt ggctgtctat gtcctcatca ttggagccat 60

cggagcgtta cgattgtttt ccaaggtgag aaaggtttaa ttg atg tct cgt tcg 115

Leu Met Ser Arg Ser

1 5

ccg gag caa cat ttg gca gaa att tca gcg ctg ctt ccc cca caa aag 163

Pro Glu Gln His Leu Ala Glu Ile Ser Ala Leu Leu Pro Pro Gln Lys

10 15 20

tcc acg ttc gtg aat ctg cgc gaa gcg ttg gga cgc cgc acg ttt tca 211

Ser Thr Phe Val Asn Leu Arg Glu Ala Leu Gly Arg Arg Thr Phe Ser

25 30 35

gcg gtc act gcg cag tgg gat tcg cca cgt ttt gat aat tcc caa atg 259

Ala Val Thr Ala Gln Trp Asp Ser Pro Arg Phe Asp Asn Ser Gln Met

40 45 50

gat ggc ttc gcg ctt ggc ccc tca cat ctt aac ggt ggc acc ttc gca 307

Asp Gly Phe Ala Leu Gly Pro Ser His Leu Asn Gly Gly Thr Phe Ala

55 60 65

gtc ggt cca acc att ccc gct ggt cat gat cct gat cag tgg tac cca 355

Val Gly Pro Thr Ile Pro Ala Gly His Asp Pro Asp Gln Trp Tyr Pro

70 75 80 85

cga ggc atc gaa aaa gac atc gcg ccg att atg acg ggt gcg cgc ctt 403

Arg Gly Ile Glu Lys Asp Ile Ala Pro Ile Met Thr Gly Ala Arg Leu

90 95 100

cct aaa aac acc gcc gcg atc att cct gtg gag aaa acc aca ccg gga 451

Pro Lys Asn Thr Ala Ala Ile Ile Pro Val Glu Lys Thr Thr Pro Gly

105 110 115

aat ttc gac gcc cca cag gta gaa atc ccc gcc acc ccg caa ggt cag 499

Asn Phe Asp Ala Pro Gln Val Glu Ile Pro Ala Thr Pro Gln Gly Gln

120 125 130

ttc ata cgg ttg cag ggt tcg gat att act gcc ggc gac gag atc att 547

Phe Ile Arg Leu Gln Gly Ser Asp Ile Thr Ala Gly Asp Glu Ile Ile

135 140 145

cca gca ggt acg gag ctt aac tcg gtg cac atc ggg gtg ttg gct agt 595

Pro Ala Gly Thr Glu Leu Asn Ser Val His Ile Gly Val Leu Ala Ser

150 155 160 165

cag tcg atc aag agc att gaa gtc gca gca aag cca cgt gtc ctc atc 643

Gln Ser Ile Lys Ser Ile Glu Val Ala Ala Lys Pro Arg Val Leu Ile

170 175 180

atc acc ggc ggg tct gaa att tca gaa cag cac gga ccc gcc acg atc 691

Ile Thr Gly Gly Ser Glu Ile Ser Glu Gln His Gly Pro Ala Thr Ile

185 190 195

cct gat gcc aac ggc cct ctg ctt cgt tcc ctg tgc gcc cgc aac aat 739

Pro Asp Ala Asn Gly Pro Leu Leu Arg Ser Leu Cys Ala Arg Asn Asn

200 205 210

atc gag gtc atc gcg gga ctg cac acc aac gac gat cct gaa cga ctc 787

Ile Glu Val Ile Ala Gly Leu His Thr Asn Asp Asp Pro Glu Arg Leu

215 220 225

cgc ttt gaa ctg gaa aac gcc att gac cag tat caa ccg gat gtc atc 835

Arg Phe Glu Leu Glu Asn Ala Ile Asp Gln Tyr Gln Pro Asp Val Ile

230 235 240 245

atc acc tct ggt ggt atc agc cac ggt aaa ttt gag gtg ttt agg cag 883

Ile Thr Ser Gly Gly Ile Ser His Gly Lys Phe Glu Val Phe Arg Gln

250 255 260

atc ctc gaa ggc acc ccg aac tcc tgg ttt gga cat gtc gat cag cag 931

Ile Leu Glu Gly Thr Pro Asn Ser Trp Phe Gly His Val Asp Gln Gln

265 270 275

cct ggc ggt cct caa ggc atc tcc act ttt gct gaa act cct gtc att 979

Pro Gly Gly Pro Gln Gly Ile Ser Thr Phe Ala Glu Thr Pro Val Ile

280 285 290

tca ctt ccc gga aat ccg att tcc acc ttg gtg agt ttc aca ctt ttc 1027

Ser Leu Pro Gly Asn Pro Ile Ser Thr Leu Val Ser Phe Thr Leu Phe

295 300 305

gtc gcg cca gcg ctc aac cgc cag ccg ctc cgc cac ctc gat gcc cgc 1075

Val Ala Pro Ala Leu Asn Arg Gln Pro Leu Arg His Leu Asp Ala Arg

310 315 320 325

atc acc gct ccg gtc cag ggc ttg caa gac aat cgc gag caa ttc ctt 1123

Ile Thr Ala Pro Val Gln Gly Leu Gln Asp Asn Arg Glu Gln Phe Leu

330 335 340

cgc ggc acc atc agt tac cgc aac ggc cac gtc ctc gcc acg cct ctc 1171

Arg Gly Thr Ile Ser Tyr Arg Asn Gly His Val Leu Ala Thr Pro Leu

345 350 355

ctg ggc acc agt tcc cac ctg ctg gtt caa gct gcc acc gca gac tgt 1219

Leu Gly Thr Ser Ser His Leu Leu Val Gln Ala Ala Thr Ala Asp Cys

360 365 370

ctg atc agg atc ccg gcg cgg act acg gtg gag gaa aac gac atc gtt 1267

Leu Ile Arg Ile Pro Ala Arg Thr Thr Val Glu Glu Asn Asp Ile Val

375 380 385

aag att tac cca ttc aac taacaggagt taatttaatg agcgagctca 1315

Lys Ile Tyr Pro Phe Asn

390 395

<210>148

<211>395

<212>PRT

<213>谷氨酸棒状杆菌

<400>148

Leu Met Ser Arg Ser Pro Glu Gln His Leu Ala Glu Ile Ser Ala Leu

1 5 10 15

Leu Pro Pro Gln Lys Ser Thr Phe Val Asn Leu Arg Glu Ala Leu Gly

20 25 30

Arg Arg Thr Phe Ser Ala Val Thr Ala Gln Trp Asp Ser Pro Arg Phe

35 40 45

Asp Asn Ser Gln Met Asp Gly Phe Ala Leu Gly Pro Ser His Leu Asn

50 55 60

Gly Gly Thr Phe Ala Val Gly Pro Thr Ile Pro Ala Gly His Asp Pro

65 70 75 80

Asp Gln Trp Tyr Pro Arg Gly Ile Glu Lys Asp Ile Ala Pro Ile Met

85 90 95

Thr Gly Ala Arg Leu Pro Lys Asn Thr Ala Ala Ile Ile Pro Val Glu

100 105 110

Lys Thr Thr Pro Gly Asn Phe Asp Ala Pro Gln Val Glu Ile Pro Ala

115 120 125

Thr Pro Gln Gly Gln Phe Ile Arg Leu Gln Gly Ser Asp Ile Thr Ala

130 135 140

Gly Asp Glu Ile Ile Pro Ala Gly Thr Glu Leu Asn Ser Val His Ile

145 150 155 160

Gly Val Leu Ala Ser Gln Ser Ile Lys Ser Ile Glu Val Ala Ala Lys

165 170 175

Pro Arg Val Leu Ile Ile Thr Gly Gly Ser Glu Ile Ser Glu Gln His

180 185 190

Gly Pro Ala Thr Ile Pro Asp Ala Asn Gly Pro Leu Leu Arg Ser Leu

195 200 205

Cys Ala Arg Asn Asn Ile Glu Val Ile Ala Gly Leu His Thr Asn Asp

210 215 220

Asp Pro Glu Arg Leu Arg Phe Glu Leu Glu Asn Ala Ile Asp Gln Tyr

225 230 235 240

Gln Pro Asp Val Ile Ile Thr Ser Gly Gly Ile Ser His Gly Lys Phe

245 250 255

Glu Val Phe Arg Gln Ile Leu Glu Gly Thr Pro Asn Ser Trp Phe Gly

260 265 270

His Val Asp Gln Gln Pro Gly Gly Pro Gln Gly Ile Ser Thr Phe Ala

275 280 285

Glu Thr Pro Val Ile Ser Leu Pro Gly Asn Pro Ile Ser Thr Leu Val

290 295 300

Ser Phe Thr Leu Phe Val Ala Pro Ala Leu Asn Arg Gln Pro Leu Arg

305 310 315 320

His Leu Asp Ala Arg Ile Thr Ala Pro Val Gln Gly Leu Gln Asp Asn

325 330 335

Arg Glu Gln Phe Leu Arg Gly Thr Ile Ser Tyr Arg Asn Gly His Val

340 345 350

Leu Ala Thr Pro Leu Leu Gly Thr Ser Ser His Leu Leu Val Gln Ala

355 360 365

Ala Thr Ala Asp Cys Leu Ile Arg Ile Pro Ala Arg Thr Thr Val Glu

370 375 380

Glu Asn Asp Ile Val Lys Ile Tyr Pro Phe Asn

385 390 395

<210>149

<211>2101

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2071)

<223>RXA04120

<400>149

caacgaaaca aatgcaagcc cccaatcatg ggtttctacc aattaaattt tctttcagaa 60

aatatctccc cacataaaag ttccttgata ggctcgagag atg aaa gtg acc caa 115

Met Lys Val Thr Gln

1 5

agc aca ttc ctt aaa tcg gta gct gcg ttc act gtc gca gcc tta acc 163

Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr Val Ala Ala Leu Thr

10 15 20

ctg acc atc tct tcg tgt tcc agc ggt gaa gac acc tcc gca agc tcc 211

Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp Thr Ser Ala Ser Ser

25 30 35

acg gat act gaa aac tcc tca acc caa gca gca gcg tct ccc cca ctt 259

Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala Ala Ser Pro Pro Leu

40 45 50

gcg cct tgt gaa ctt ccc gcc gac gct tct gct gaa gag gaa gta gaa 307

Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala Glu Glu Glu Val Glu

55 60 65

ggc act cac aca ggt gaa gat att tct gtt gcc ccg gaa atc ggt acc 355

Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala Pro Glu Ile Gly Thr

70 75 80 85

ggc tac cgc gag ggc atg acc cct gtt caa acc caa ggt tat gcg gtg 403

Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr Gln Gly Tyr Ala Val

90 95 100

gca act gca aac ccc atc gct tct gaa gca gcc tgc gcg gtg tta aga 451

Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala Cys Ala Val Leu Arg

105 110 115

gaa ggc ggc act gca gct gat gct ctt gtc acc gcg cag ttt gtt ttg 499

Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr Ala Gln Phe Val Leu

120 125 130

gga ctg acg gaa ccg cag tcg tct ggc ctt ggt ggt ggc gga tac att 547

Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly Gly Gly Gly Tyr Ile

135 140 145

ctg tac tac gac gcc gaa gcc aat gcg gtg aca gcc att gat ggc cgt 595

Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr Ala Ile Asp Gly Arg

150 155 160 165

gaa aca gcg cca gtt gct gct gat gaa aac tat ctc att cat gtt tct 643

Glu Thr Ala Pro Val Ala Ala Asp Glu Asn Tyr Leu Ile His Val Ser

170 175 180

gca gag gat caa acg gca cct gtt cct gat gcc cga cgt tcc ggc agg 691

Ala Glu Asp Gln Thr Ala Pro Val Pro Asp Ala Arg Arg Ser Gly Arg

185 190 195

tca att ggt gtg cca gga atc gtg gca gcc ctt gga cag ctg cat gat 739

Ser Ile Gly Val Pro Gly Ile Val Ala Ala Leu Gly Gln Leu His Asp

200 205 210

tca ttc gga aag acc tcc tgg cag gac gtg ctg aca act ccg cag cag 787

Ser Phe Gly Lys Thr Ser Trp Gln Asp Val Leu Thr Thr Pro Gln Gln

215 220 225

ctc gca act gat ggt ttt tcc atc agc cct cgc atg tca gca tca att 835

Leu Ala Thr Asp Gly Phe Ser Ile Ser Pro Arg Met Ser Ala Ser Ile

230 235 240 245

gct aac tcc gct gag gat ctc tcc cac gat ccg gaa gct gcc gca tat 883

Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro Glu Ala Ala Ala Tyr

250 255 260

ttc ctt gat gaa aac ggt gat gcg aag gca ccc ggc aca ctt tta caa 931

Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro Gly Thr Leu Leu Gln

265 270 275

aac cct gac tat gca gaa acg att cgt ctc atc tct gaa ggt ggc ccc 979

Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile Ser Glu Gly Gly Pro

280 285 290

gat gcg ttc tac acg ggt gag att gca gca gac atc gtg gaa cgc gcc 1027

Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp Ile Val Glu Arg Ala

295 300 305

acc cgt gag gtt gac ggt ttc aca cca tca ctg atg agc acg gca gat 1075

Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu Met Ser Thr Ala Asp

310 315 320 325

ttg gct gcc tac act ccg gaa act cgt gaa gct ttg tgt gct ccc tac 1123

Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala Leu Cys Ala Pro Tyr

330 335 340

cgc gac aag att gtt tgt ggc atg cca ccg tca tca tcg ggt ggc gtc 1171

Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser Ser Ser Gly Gly Val

345 350 355

aca gtg atg gaa acc ctg ggt atc ttg aac aac ttt gat ctc gcc caa 1219

Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn Phe Asp Leu Ala Gln

360 365 370

tac cca ccc act gag gtt ggt ttg gat ggc gga ttg cca aat gcg gaa 1267

Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly Leu Pro Asn Ala Glu

375 380 385

gct gtt cac ctg att tca gag gct gag cgc ctg gct tat gct gat cgc 1315

Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu Ala Tyr Ala Asp Arg

390 395 400 405

gat gct tac atc ggt gat cct gct ttc gtg gaa gtt cca gca ggt ggt 1363

Asp Ala Tyr Ile Gly Asp Pro Ala Phe Val Glu Val Pro Ala Gly Gly

410 415 420

gtc gaa gag ttg atc agc gat gtc tac acg ggt gaa cgc tca gaa ctt 1411

Val Glu Glu Leu Ile Ser Asp Val Tyr Thr Gly Glu Arg Ser Glu Leu

425 430 435

att gat cca gag cat tca atg ggt cag gca act gct ggt ctg agc cag 1459

Ile Asp Pro Glu His Ser Met Gly Gln Ala Thr Ala Gly Leu Ser Gln

440 445 450

gaa cca gtc atg gct gcc ctg ccg gaa agt ggc acc agc cat att tcc 1507

Glu Pro Val Met Ala Ala Leu Pro Glu Ser Gly Thr Ser His Ile Ser

455 460 465

atc atc gat tcc tat ggc aac gca gca tcg ttg acc acc agt gtg gaa 1555

Ile Ile Asp Ser Tyr Gly Asn Ala Ala Ser Leu Thr Thr Ser Val Glu

470 475 480 485

gct gct ttc ggt tcc ttc cac ttc acc cgt ggt ttc att ttg aat aat 1603

Ala Ala Phe Gly Ser Phe His Phe Thr Arg Gly Phe Ile Leu Asn Asn

490 495 500

cag ctg aca gat ttc tcc gct gaa cca ctt gat gag gac ggc gag ccc 1651

Gln Leu Thr Asp Phe Ser Ala Glu Pro Leu Asp Glu Asp Gly Glu Pro

505 510 515

gtg gcc aat cgt gtc gag tca gca aag cgc cca cgg tct tcc atg tcg 1699

Val Ala Asn Arg Val Glu Ser Ala Lys Arg Pro Arg Ser Ser Met Ser

520 525 530

cca atg cta gtg ttc aac gcc agt ggc gat ggt gaa atc gcg gat ctg 1747

Pro Met Leu Val Phe Asn Ala Ser Gly Asp Gly Glu Ile Ala Asp Leu

535 540 545

aat atg gtg ctg ggc tcc cct ggc gga tcc ttg att att cag tac gtg 1795

Asn Met Val Leu Gly Ser Pro Gly Gly Ser Leu Ile Ile Gln Tyr Val

550 555 560 565

gtg aaa acc ctg gtc aac atc atc gac tgg gat atg gat cca cag cag 1843

Val Lys Thr Leu Val Asn Ile Ile Asp Trp Asp Met Asp Pro Gln Gln

570 575 580

gca gtg tct gcg ccc aac ttt ggt gcg atg aac cag cct aag act gga 1891

Ala Val Ser Ala Pro Asn Phe Gly Ala Met Asn Gln Pro Lys Thr Gly

585 590 595

ctg gga agc gag cat ccg ctg atc gcc aat gat tca gca gag ctt gta 1939

Leu Gly Ser Glu His Pro Leu Ile Ala Asn Asp Ser Ala Glu Leu Val

600 605 610

tct gaa ctg gaa agc aaa ggc cac gaa gtt aat gtg ggc gag caa tcc 1987

Ser Glu Leu Glu Ser Lys Gly His Glu Val Asn Val Gly Glu Gln Ser

615 620 625

agt ggc cta tcg gcg ttg gtg aaa aac ggc gac acc att gtc ggt ggc 2035

Ser Gly Leu Ser Ala Leu Val Lys Asn Gly Asp Thr Ile Val Gly Gly

630 635 640 645

gcc gat cca cgt aga gaa ggc gtg gtc ttg ggt ggc taattagcgc 2081

Ala Asp Pro Arg Arg Glu Gly Val Val Leu Gly Gly

650 655

gcagaccacg cagtccacag 2101

<210>150

<211>657

<212>PRT

<213>谷氨酸棒状杆菌

<400>150

Met Lys Val Thr Gln Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr

1 5 10 15

Val Ala Ala Leu Thr Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp

20 25 30

Thr Ser Ala Ser Ser Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala

35 40 45

Ala Ser Pro Pro Leu Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala

50 55 60

Glu Glu Glu Val Glu Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala

65 70 75 80

Pro Glu Ile Gly Thr Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr

85 90 95

Gln Gly Tyr Ala Val Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala

100 105 110

Cys Ala Val Leu Arg Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr

115 120 125

Ala Gln Phe Val Leu Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly

130 135 140

Gly Gly Gly Tyr Ile Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr

145 150 155 160

Ala Ile Asp Gly Arg Glu Thr Ala Pro Val Ala Ala Asp Glu Asn Tyr

165 170 175

Leu Ile His Val Ser Ala Glu Asp Gln Thr Ala Pro Val Pro Asp Ala

180 185 190

Arg Arg Ser Gly Arg Ser Ile Gly Val Pro Gly Ile Val Ala Ala Leu

195 200 205

Gly Gln Leu His Asp Ser Phe Gly Lys Thr Ser Trp Gln Asp Val Leu

210 215 220

Thr Thr Pro Gln Gln Leu Ala Thr Asp Gly Phe Ser Ile Ser Pro Arg

225 230 235 240

Met Ser Ala Ser Ile Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro

245 250 255

Glu Ala Ala Ala Tyr Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro

260 265 270

Gly Thr Leu Leu Gln Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile

275 280 285

Ser Glu Gly Gly Pro Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp

290 295 300

Ile Val Glu Arg Ala Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu

305 310 315 320

Met Ser Thr Ala Asp Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala

325 330 335

Leu Cys Ala Pro Tyr Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser

340 345 350

Ser Ser Gly Gly Val Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn

355 360 365

Phe Asp Leu Ala Gln Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly

370 375 380

Leu Pro Asn Ala Glu Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu

385 390 395 400

Ala Tyr Ala Asp Arg Asp Ala Tyr Ile Gly Asp Pro Ala Phe Val Glu

405 410 415

Val Pro Ala Gly Gly Val Glu Glu Leu Ile Ser Asp Val Tyr Thr Gly

420 425 430

Glu Arg Ser Glu Leu Ile Asp Pro Glu His Ser Met Gly Gln Ala Thr

435 440 445

Ala Gly Leu Ser Gln Glu Pro Val Met Ala Ala Leu Pro Glu Ser Gly

450 455 460

Thr Ser His Ile Ser Ile Ile Asp Ser Tyr Gly Asn Ala Ala Ser Leu

465 470 475 480

Thr Thr Ser Val Glu Ala Ala Phe Gly Ser Phe His Phe Thr Arg Gly

485 490 495

Phe Ile Leu Asn Asn Gln Leu Thr Asp Phe Ser Ala Glu Pro Leu Asp

500 505 510

Glu Asp Gly Glu Pro Val Ala Asn Arg Val Glu Ser Ala Lys Arg Pro

515 520 525

Arg Ser Ser Met Ser Pro Met Leu Val Phe Asn Ala Ser Gly Asp Gly

530 535 540

Glu Ile Ala Asp Leu Asn Met Val Leu Gly Ser Pro Gly Gly Ser Leu

545 550 555 560

Ile Ile Gln Tyr Val Val Lys Thr Leu Val Asn Ile Ile Asp Trp Asp

565 570 575

Met Asp Pro Gln Gln Ala Val Ser Ala Pro Asn Phe Gly Ala Met Asn

580 585 590

Gln Pro Lys Thr Gly Leu Gly Ser Glu His Pro Leu Ile Ala Asn Asp

595 600 505

Ser Ala Glu Leu Val Ser Glu Leu Glu Ser Lys Gly His Glu Val Asn

610 615 620

Val Gly Glu Gln Ser Ser Gly Leu Ser Ala Leu Val Lys Asn Gly Asp

625 630 635 640

Thr Ile Val Gly Gly Ala Asp Pro Arg Arg Glu Gly Val Val Leu Gly

645 650 655

Gly

<210>151

<211>886

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(856)

<223>RXA04173

<400>151

actgtccgaa gtattggatc tggtcgaggg aactgtccgt aaacgcgact aaacgacccc 60

tgattcacac tttcagacta caagaactag actaagcggt atg gtt tca gtt ctt 115

Met Val Ser Val Leu

1 5

tta att cag ccc cgt cag gga gaa gca gtc gcc gca gct gag cga cgt 163

Leu Ile Gln Pro Arg Gln Gly Glu Ala Val Ala Ala Ala Glu Arg Arg

10 15 20

gac ttt ttg cag gcc acc ggt ctt aag cct caa gaa ctg acc tcc cga 211

Asp Phe Leu Gln Ala Thr Gly Leu Lys Pro Gln Glu Leu Thr Ser Arg

25 30 35

atg ttg gat acc acc act tct cga att ggc agt ctg gaa ggt ttc gac 259

Met Leu Asp Thr Thr Thr Ser Arg Ile Gly Ser Leu Glu Gly Phe Asp

40 45 50

ggc gtg att gtg ggc gga agc cca ctg aat gcc acc aac ttt gag tac 307

Gly Val Ile Val Gly Gly Ser Pro Leu Asn Ala Thr Asn Phe Glu Tyr

55 60 65

agc gat tgg caa cgc cac gtc cac cgc gaa ttg tcc ttg ctg atc aat 355

Ser Asp Trp Gln Arg His Val His Arg Glu Leu Ser Leu Leu Ile Asn

70 75 80 85

cac cca ctg cca aca atc ttt gtc tgc tac ggc aat acc ttt ttg acc 403

His Pro Leu Pro Thr Ile Phe Val Cys Tyr Gly Asn Thr Phe Leu Thr

90 95 100

ttc ttc tct ggc gga cag att ggt cgc aca cac ccc gaa gat tcc ggc 451

Phe Phe Ser Gly Gly Gln Ile Gly Arg Thr His Pro Glu Asp Ser Gly

105 110 115

gcc acc aca gtg ttg cta act gac gcc ggc aaa cga gac gta ctc act 499

Ala Thr Thr Val Leu Leu Thr Asp Ala Gly Lys Arg Asp Val Leu Thr

120 125 130

caa gac cta ccg gat agc ttt acg tcc ttt act ggt cac acg gaa aac 547

Gln Asp Leu Pro Asp Ser Phe Thr Ser Phe Thr Gly His Thr Glu Asn

135 140 145

tcc gta gcg ccc gcc cct gga cac gtg gtg ttg gca acg gga cca acc 595

Ser Val Ala Pro Ala Pro Gly His Val Val Leu Ala Thr Gly Pro Thr

150 155 160 165

tgc ccc atc cag atg ctg cgc gcc aac aag aac acc tgg tca gtt caa 643

Cys Pro Ile Gln Met Leu Arg Ala Asn Lys Asn Thr Trp Ser Val Gln

170 175 180

ttc cat gcg gat atg gat gcc gta ggc atg aaa aac cgc atg gat ttt 691

Phe His Ala Asp Met Asp Ala Val Gly Met Lys Asn Arg Met Asp Phe

185 190 195

tac tcc aac tac ggc tac ttc tcc cca gaa gat tat gac cgc atc att 739

Tyr Ser Asn Tyr Gly Tyr Phe Ser Pro Glu Asp Tyr Asp Arg Ile Ile

200 205 210

gca gag cta ccc tct gtt gac tcc att tat gcc aac agg gtg ctc cgc 787

Ala Glu Leu Pro Ser Val Asp Ser Ile Tyr Ala Asn Arg Val Leu Arg

215 220 225

aac ttc gtg gag gtc tgc gaa gga att cgt gtt gct gat ggt gct gag 835

Asn Phe Val Glu Val Cys Glu Gly Ile Arg Val Ala Asp Gly Ala Glu

230 235 240 245

cac caa ctc cca aag ctt aac taatcgagga gactggtgat tcgccatcca 886

His Gln Leu Pro Lys Leu Asn

250

<210>152

<211>252

<212>PRT

<213>谷氨酸棒状杆菌

<400>152

Met Val Ser Val Leu Leu Ile Gln Pro Arg Gln Gly Glu Ala Val Ala

1 5 10 15

Ala Ala Glu Arg Arg Asp Phe Leu Gln Ala Thr Gly Leu Lys Pro Gln

20 25 30

Glu Leu Thr Ser Arg Met Leu Asp Thr Thr Thr Ser Arg Ile Gly Ser

35 40 45

Leu Glu Gly Phe Asp Gly Val Ile Val Gly Gly Ser Pro Leu Asn Ala

50 55 60

Thr Asn Phe Glu Tyr Ser Asp Trp Gln Arg His Val His Arg Glu Leu

65 70 75 80

Ser Leu Leu Ile Asn His Pro Leu Pro Thr Ile Phe Val Cys Tyr Gly

85 90 95

Asn Thr Phe Leu Thr Phe Phe Ser Gly Gly Gln Ile Gly Arg Thr His

100 105 110

Pro Glu Asp Ser Gly Ala Thr Thr Val Leu Leu Thr Asp Ala Gly Lys

115 120 125

Arg Asp Val Leu Thr Gln Asp Leu Pro Asp Ser Phe Thr Ser Phe Thr

130 135 140

Gly His Thr Glu Asn Ser Val Ala Pro Ala Pro Gly His Val Val Leu

145 150 155 160

Ala Thr Gly Pro Thr Cys Pro Ile Gln Met Leu Arg Ala Asn Lys Asn

165 170 175

Thr Trp Ser Val Gln Phe His Ala Asp Met Asp Ala Val Gly Met Lys

180 185 190

Asn Arg Met Asp Phe Tyr Ser Asn Tyr Gly Tyr Phe Ser Pro Glu Asp

195 200 205

Tyr Asp Arg Ile Ile Ala Glu Leu Pro Ser Val Asp Ser Ile Tyr Ala

210 215 220

Asn Arg Val Leu Arg Asn Phe Val Glu Val Cys Glu Gly Ile Arg Val

225 230 235 240

Ala Asp Gly Ala Glu His Gln Leu Pro Lys Leu Asn

245 250

<210>153

<211>2959

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2929)

<223>RXA04174

<400>153

ccttttccac agttctgtgg aatgagaatc cgatgttttt ctcacgccgg ctcagccgaa 60

gcagacgccg tcgcgaaatc tcaccctaaa aaagttagaa ttg gag ctc act gtg 115

Leu Glu Leu Thr Val

1 5

act gaa agc aag aac tcc ttc aat gct aag agc acc ctt gaa gtt ggc 163

Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Ser Thr Leu Glu Val Gly

10 15 20

gac aag tcc tat gac tac ttc gcc ctc tct gca gtg cct ggc atg gag 211

Asp Lys Ser Tyr Asp Tyr Phe Ala Leu Ser Ala Val Pro Gly Met Glu

25 30 35

aag ctg ccg tac tcc ctc aag gtt ctc gga gag aac ctt ctt cgt acc 259

Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg Thr

40 45 50

gaa gac ggc gca aac atc acc aac gag cac att gag gct atc gcc aac 307

Glu Asp Gly Ala Asn Ile Thr Asn Glu His Ile Glu Ala Ile Ala Asn

55 60 65

tgg gat gca tct tcc gat cca agc atc gaa atc cag ttc acc cca gcc 355

Trp Asp Ala Ser Ser Asp Pro Ser Ile Glu Ile Gln Phe Thr Pro Ala

70 75 80 85

cgt gtt ctc atg cag gac ttc acc ggt gtc cct tgt gta gtt gac ctc 403

Arg Val Leu Met Gln Asp Phe Thr Gly Val Pro Cys Val Val Asp Leu

90 95 100

gca acc atg cgt gag gca gtt gct gca ctc ggt ggc gac cct aac gac 451

Ala Thr Met Arg Glu Ala Val Ala Ala Leu Gly Gly Asp Pro Asn Asp

105 110 115

gtc aac cca ctg aac cca gcc gag atg gtc att gac cac tcc gtc atc 499

Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val Ile

120 125 130

gtg gag gct ttc ggc cgc cca gat gca ctg gct aag aac gtt gag atc 547

Val Glu Ala Phe Gly Arg Pro Asp Ala Leu Ala Lys Asn Val Glu Ile

135 140 145

gag tac gag cgc aac gag gag cgt tac cag ttc ctg cgt tgg ggt tcc 595

Glu Tyr Glu Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly Ser

150 155 160 165

gag tcc ttc tcc aac ttc cgc gtt gtt cct cca gga acc ggt atc gtc 643

Glu Ser Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile Val

170 175 180

cac cag gtc aac att gag tac ttg gct cgc gtc gtc ttc gac aac gag 691

His Gln Val Asn Ile Glu Tyr Leu Ala Arg Val Val Phe Asp Asn Glu

185 190 195

ggc ctt gca tac cca gat acc tgc atc ggt acc gac tcc cac acc acc 739

Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr Thr

200 205 210

atg gaa aac ggc ctg ggc atc ctg ggc tgg ggc gtt ggt ggc att gag 787

Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile Glu

215 220 225

gct gaa gca gca atg ctc ggc cag cca gtg tcc atg ctg atc cct cgc 835

Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro Arg

230 235 240 245

gtt gtt ggc ttc aag ttg acc ggc gag atc cca gta ggc gtt acc gca 883

Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Val Gly Val Thr Ala

250 255 260

act gac gtt gtg ctg acc atc acc gaa atg ctg cgc gac cac ggc gtc 93l

Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu Arg Asp His Gly Val

265 270 275

gtc cag aag ttc gtt gag ttc tac ggc tcc ggt gtt aag gct gtt cca 979

Val Gln Lys Phe Val Glu Phe Tyr Gly Ser Gly Val Lys Ala Val Pro

280 285 290

ctg gct aac cgt gca acc atc ggc aac atg tcc cca gag ttc ggc tcc 1027

Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser Pro Glu Phe Gly Ser

295 300 305

acc tgt gcg atg ttc cca atc gac gag gag acc acc aag tac ctg cgc 1075

Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr Thr Lys Tyr Leu Arg

310 315 320 325

ctc acc ggc cgc cca gaa gag cag gtt gca ctg gtc gag gct tac gcc 1123

Leu Thr Gly Arg Pro Glu Glu Gln Val Ala Leu Val Glu Ala Tyr Ala

330 335 340

aag gcg cag ggc atg tgg ctc gac gag gac acc gtt gaa gct gag tac 117l

Lys Ala Gln Gly Met Trp Leu Asp Glu Asp Thr Val Glu Ala Glu Tyr

345 350 355

tcc gag tac ctc gag ctg gac ctg tcc acc gtt gtt cct tcc atc gct 1219

Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val Val Pro Ser Ile Ala

360 365 370

ggc cct aag cgc cca cag gac cgc atc ctt ctc tcc gag gca aag gag 1267

Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu Ser Glu Ala Lys Glu

375 380 385

cag ttc cgt aag gat ctg cca acc tac acc gac gac gct gtt tcc gta 1315

Gln Phe Arg Lys Asp Leu Pro Thr Tyr Thr Asp Asp Ala Val Ser Val

390 395 400 405

gac acc tcc atc cct gca acc cgc atg gtt aac gaa ggt ggc gga cag 1363

Asp Thr Ser Ile Pro Ala Thr Arg Met Val Asn Glu Gly Gly Gly Gln

410 415 420

cct gaa ggc ggc gtc gaa gct gac aac tac aac gct tcc tgg gct ggc 1411

Pro Glu Gly Gly Val Glu Ala Asp Asn Tyr Asn Ala Ser Trp Ala Gly

425 430 435

tcc ggc gag tcc ttg gct act ggc gca gaa gga cgt cct tcc aag cca 1459

Ser Gly Glu Ser Leu Ala Thr Gly Ala Glu Gly Arg Pro Ser Lys Pro

440 445 450

gtc acc gtt gca tcc cca cag ggt ggc gag tac acc atc gac cac ggc 1507

Val Thr Val Ala Ser Pro Gln Gly Gly Glu Tyr Thr Ile Asp His Gly

455 460 465

atg gtt gca att gca tcc atc acc tct tgc acc aac acc tct aac cca 1555

Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr Asn Thr Ser Asn Pro

470 475 480 485

tcc gtg atg atc ggc gct ggc ctg atc gca cgt aag gca gca gaa aag 1603

Ser Val Met Ile Gly Ala Gly Leu Ile Ala Arg Lys Ala Ala Glu Lys

490 495 500

ggc ctc aag tcc aag cct tgg gtt aag acc atc tgt gca cca ggt tcc 1651

Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile Cys Ala Pro Gly Ser

505 510 515

cag gtt gtc gac ggc tac tac cag cgc gca gac ctc tgg aag gac ctt 1699

Gln Val Val Asp Gly Tyr Tyr Gln Arg Ala Asp Leu Trp Lys Asp Leu

520 525 530

gag gcc atg ggc ttc tac ctc tcc ggc ttc ggc tgc acc acc tgt att 1747

Glu Ala Met Gly Phe Tyr Leu Ser Gly Phe Gly Cys Thr Thr Cys Ile

535 540 545

ggt aac tcc ggc cca ctg cca gag gaa atc tcc gct gcg atc aac gag 1795

Gly Asn Ser Gly Pro Leu Pro Glu Glu Ile Ser Ala Ala Ile Asn Glu

550 555 560 565

cac gac ctg acc gca acc gca gtt ttg tcc ggt aac cgt aac ttc gag 1843

His Asp Leu Thr Ala Thr Ala Val Leu Ser Gly Asn Arg Asn Phe Glu

570 575 580

gga cgt atc tcc cct gac gtt aag atg aac tac ctg gca tcc cca atc 1891

Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr Leu Ala Ser Pro Ile

585 590 595

atg gtc att gct tac gca atc gct ggc acc atg gac ttc gac ttc gag 1939

Met Val Ile Ala Tyr Ala Ile Ala Gly Thr Met Asp Phe Asp Phe Glu

600 605 610

aac gaa gct ctt gga cag gac cag gac ggc aac gac gtc ttc ctg aag 1987

Asn Glu Ala Leu Gly Gln Asp Gln Asp Gly Asn Asp Val Phe Leu Lys

615 620 625

gac atc tgg cct tcc acc gag gaa atc gaa gac acc atc cag cag gca 2035

Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Asp Thr Ile Gln Gln Ala

630 635 640 645

atc tcc cgt gag ctt tac gaa gct gac tac gca gat gtc ttc aag ggt 2083

Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala Asp Val Phe Lys Gly

650 655 660

gac aag cag tgg cag gaa ctc gat gtt cct acc ggt gac acc ttc gag 2131

Asp Lys Gln Trp Gln Glu Leu Asp Val Pro Thr Gly Asp Thr Phe Glu

665 670 675

tgg gac gag aac tcc acc tac atc cgc aag gca cct tac ttc gac ggc 2179

Trp Asp Glu Asn Ser Thr Tyr Ile Arg Lys Ala Pro Tyr Phe Asp Gly

680 685 690

atg cct gtc gag cca gtg gca gtc acc gac atc cag ggc gca cgc gtt 2227

Met Pro Val Glu Pro Val Ala Val Thr Asp Ile Gln Gly Ala Arg Val

695 700 705

ctg gct aag ctc ggc gac tct gtc acc acc gac cac atc tcc cct gct 2275

Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp His Ile Ser Pro Ala

710 715 720 725

tcc tcc att aag cca ggt acc cct gca gct cag tac ttg gat gag cac 2323

Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln Tyr Leu Asp Glu His

730 735 740

ggt gtg gaa cgc cac gac tac aac tcc ctg ggt tcc agg cgt ggt aac 237l

Gly Val Glu Arg His Asp Tyr Asn Ser Leu Gly Ser Arg Arg Gly Asn

745 750 755

cac gag gtc atg atg cgc ggc acc ttc gcc aac atc cgc ctc cag aac 2419

His Glu Val Met Met Arg Gly Thr Phe Ala Asn Ile Arg Leu Gln Asn

760 765 770

cag ctg gtt gac atc gca ggt ggc tac acc cgc gac ttc acc cag gag 2467

Gln Leu Val Asp Ile Ala Gly Gly Tyr Thr Arg Asp Phe Thr Gln Glu

775 780 785

ggt gct cca cag gcg ttc atc tac gac gct tcc gtc aac tac aag gct 2515

Gly Ala Pro Gln Ala Phe Ile Tyr Asp Ala Ser Val Asn Tyr Lys Ala

790 795 800 805

gct ggc att ccg ctg gtc gtc ttg ggc ggc aag gag tac ggc acc ggt 2563

Ala Gly Ile Pro Leu Val Val Leu Gly Gly Lys Glu Tyr Gly Thr Gly

810 815 820

tct tcc cgt gac tgg gca gct aag ggc act aac ctg ctc gga att cgc 2611

Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Asn Leu Leu Gly Ile Arg

825 830 835

gca gtt atc acc gag tcc ttc gag cgt att cac cgc tcc aac ctc atc 2659

Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His Arg Ser Asn Leu Ile

840 845 850

ggt atg ggc gtt gtc cca ctg cag ttc cct gca ggc gaa tcc cac gag 2707

Gly Met Gly Val Val Pro Leu Gln Phe Pro Ala Gly Glu Ser His Glu

855 860 865

tcc ctg ggc ctt gac ggc acc gag acc ttc gac atc acc gga ctg acc 2755

Ser Leu Gly Leu Asp Gly Thr Glu Thr Phe Asp Ile Thr Gly Leu Thr

870 875 880 885

gca ctc aac gag ggc gag act cct aag act gtc aag gtc acc gca acc 2803

Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val Lys Val Thr Ala Thr

890 895 900

aag gag aac ggc gac gtc gtc gag ttc gac gca gtt gtc cgc atc gac 2851

Lys Glu Asn Gly Asp Val Val Glu Phe Asp Ala Val Val Arg Ile Asp

905 910 915

acc cca ggt gag gct gac tac tac cgc cac ggc ggc atc ctg cag tac 2899

Thr Pro Gly Glu Ala Asp Tyr Tyr Arg His Gly Gly Ile Leu Gln Tyr

920 925 930

gtg ctg cgt cag atg gct gct tct tct aag taattagaag acttttgatg 2949

Val Leu Arg Gln Met Ala Ala Ser Ser Lys

935 940

tggagtttcc 2959

<210>154

<211>943

<212>PRT

<213>谷氨酸棒状杆菌

<400>154

Leu Glu Leu Thr Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Ser

1 5 10 15

Thr Leu Glu Val Gly Asp Lys Ser Tyr Asp Tyr Phe Ala Leu Ser Ala

20 25 30

Val Pro Gly Met Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu

35 40 45

Asn Leu Leu Arg Thr Glu Asp Gly Ala Asn Ile Thr Asn Glu His Ile

50 55 60

Glu Ala Ile Ala Asn Trp Asp Ala Ser Ser Asp Pro Ser Ile Glu Ile

65 70 75 80

Gln Phe Thr Pro Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Pro

85 90 95

Cys Val Val Asp Leu Ala Thr Met Arg Glu Ala Val Ala Ala Leu Gly

100 105 110

Gly Asp Pro Asn Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile

115 120 125

Asp His Ser Val Ile Val Glu Ala Phe Gly Arg Pro Asp Ala Leu Ala

130 135 140

Lys Asn Val Glu Ile Glu Tyr Glu Arg Asn Glu Glu Arg Tyr Gln Phe

145 150 155 160

Leu Arg Trp Gly Ser Glu Ser Phe Ser Asn Phe Arg Val Val Pro Pro

165 170 175

Gly Thr Gly Ile Val His Gln Val Asn Ile Glu Tyr Leu Ala Arg Val

180 185 190

Val Phe Asp Asn Glu Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr

195 200 205

Asp Ser His Thr Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly

210 215 220

Val Gly Gly Ile Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser

225 230 235 240

Met Leu Ile Pro Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro

245 250 255

Val Gly Val Thr Ala Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu

260 265 270

Arg Asp His Gly Val Val Gln Lys Phe Val Glu Phe Tyr Gly Ser Gly

275 280 285

Val Lys Ala Val Pro Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser

290 295 300

Pro Glu Phe Gly Ser Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr

305 310 315 320

Thr Lys Tyr Leu Arg Leu Thr Gly Arg Pro Glu Glu Gln Val Ala Leu

325 330 335

Val Glu Ala Tyr Ala Lys Ala Gln Gly Met Trp Leu Asp Glu Asp Thr

340 345 350

Val Glu Ala Glu Tyr Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val

355 360 365

Val Pro Ser Ile Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu

370 375 380

Ser Glu Ala Lys Glu Gln Phe Arg Lys Asp Leu Pro Thr Tyr Thr Asp

385 390 395 400

Asp Ala Val Ser Val Asp Thr Ser Ile Pro Ala Thr Arg Met Val Asn

405 410 415

Glu Gly Gly Gly Gln Pro Glu Gly Gly Val Glu Ala Asp Asn Tyr Asn

420 425 430

Ala Ser Trp Ala Gly Ser Gly Glu Ser Leu Ala Thr Gly Ala Glu Gly

435 440 445

Arg Pro Ser Lys Pro Val Thr Val Ala Ser Pro Gln Gly Gly Glu Tyr

450 455 460

Thr Ile Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr

465 470 475 480

Asn Thr Ser Asn Pro Ser Val Met Ile Gly Ala Gly Leu Ile Ala Arg

485 490 495

Lys Ala Ala Glu Lys Gly Leu Lys Ser Lys Pro Trp Val lys Thr Ile

500 505 510

Cys Ala Pro Gly Ser Gln Val Val Asp Gly Tyr Tyr Gln Arg Ala Asp

515 520 525

Leu Trp Lys Asp Leu Glu Ala Met Gly Phe Tyr Leu Ser Gly Phe Gly

530 535 540

Cys Thr Thr Cys Ile Gly Asn Ser Gly Pro Leu Pro Glu Glu Ile Ser

545 550 555 560

Ala Ala Ile Asn Glu His Asp Leu Thr Ala Thr Ala Val Leu Ser Gly

565 570 575

Asn Arg Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr

580 585 590

Leu Ala Ser Pro Ile Met Val Ile Ala Tyr Ala Ile Ala Gly Thr Met

595 600 605

Asp Phe Asp Phe Glu Asn Glu Ala Leu Gly Gln Asp Gln Asp Gly Asn

610 615 620

Asp Val Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Asp

625 630 635 640

Thr Ile Gln Gln Ala Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala

645 650 655

Asp Val Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Val Pro Thr

660 665 670

Gly Asp Thr Phe Glu Trp Asp Glu Asn Ser Thr Tyr Ile Arg Lys Ala

675 680 685

Pro Tyr Phe Asp Gly Met Pro Val Glu Pro Val Ala Val Thr Asp Ile

690 695 700

Gln Gly Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp

705 710 715 720

His Ile Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln

725 730 735

Tyr Leu Asp Glu His Gly Val Glu Arg His Asp Tyr Asn Ser Leu Gly

740 745 750

Ser Arg Arg Gly Asn His Glu Val Met Met Arg Gly Thr Phe Ala Asn

755 760 765

Ile Arg Leu Gln Asn Gln Leu Val Asp Ile Ala Gly Gly Tyr Thr Arg

770 775 780

Asp Phe Thr Gln Glu Gly Ala Pro Gln Ala Phe Ile Tyr Asp Ala Ser

785 790 795 800

Val Asn Tyr Lys Ala Ala Gly Ile Pro Leu Val Val Leu Gly Gly Lys

805 810 815

Glu Tyr Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Asn

820 825 830

Leu Leu Gly Ile Arg Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His

835 840 845

Arg Ser Asn Leu Ile Gly Met Gly Val Val Pro Leu Gln Phe Pro Ala

850 855 860

Gly Glu Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Thr Phe Asp

865 870 875 880

Ile Thr Gly Leu Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val

885 890 895

Lys Val Thr Ala Thr Lys Glu Asn Gly Asp Val Val Glu Phe Asp Ala

900 905 910

Val Val Arg Ile Asp Thr Pro Gly Glu Ala Asp Tyr Tyr Arg His Gly

915 920 925

Gly Ile Leu Gln Tyr Val Leu Arg Gln Met Ala Ala Ser Ser Lys

930 935 940

<210>155

<211>3793

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3763)

<223>RXA04186

<400>155

agactagtgg cgctttgcct gtgttgctta ggcggcgttg aaaatgaact acgaatgaaa 60

agttcgggaa ttgtctaatc cgtactaagc tgtctacaca atg tct act tca gtt 115

Met Ser Thr Ser Val

1 5

act tca cca gcc cac aac aac gca cat tcc tcc gaa ttt ttg gat gcg 163

Thr Ser Pro Ala His Asn Asn Ala His Ser Ser Glu Phe Leu Asp Ala

10 15 20

ttg gca aac cat gtg ttg atc ggc gac ggc gcc atg ggc acc cag ctc 21l

Leu Ala Asn His Val Leu Ile Gly Asp Gly Ala Met Gly Thr Gln Leu

25 30 35

caa ggc ttt gac ctg gac gtg gaa aag gat ttc ctt gat ctg gag ggg 259

Gln Gly Phe Asp Leu Asp Val Glu Lys Asp Phe Leu Asp Leu Glu Gly

40 45 50

tgt aat gag att ctc aac gac acc cgc cct gat gtg ttg agg cag att 307

Cys Asn Glu Ile Leu Asn Asp Thr Arg Pro Asp Val Leu Arg Gln Ile

55 60 65

cac cgc gcc tac ttt gag gcg gga gct gac ttg gtt gag acc aat act 355

His Arg Ala Tyr Phe Glu Ala Gly Ala Asp Leu Val Glu Thr Asn Thr

70 75 80 85

ttt ggt tgc aac ctg ccg aac ttg gcg gat tat gac atc gct gat cgt 403

Phe Gly Cys Asn Leu Pro Asn Leu Ala Asp Tyr Asp Ile Ala Asp Arg

90 95 100

tgc cgt gag ctt gcc tac aag ggc act gca gtg gct agg gaa gtg gct 451

Cys Arg Glu Leu Ala Tyr Lys Gly Thr Ala Val Ala Arg Glu Val Ala

105 110 115

gat gag atg ggg ccg ggc cga aac ggc atg cgg cgt ttc gtg gtt ggt 499

Asp Glu Met Gly Pro Gly Arg Asn Gly Met Arg Arg Phe Val Val Gly

120 125 130

tcc ctg gga cct gga acg aag ctt cca tcg ctg ggc cat gca ccg tat 547

Ser Leu Gly Pro Gly Thr Lys Leu Pro Ser Leu Gly His Ala Pro Tyr

135 140 145

gca gat ttg cgt ggg cac tac aag gaa gca gcg ctt ggc atc atc gac 595

Ala Asp Leu Arg Gly His Tyr Lys Glu Ala Ala Leu Gly Ile Ile Asp

150 155 160 165

ggt ggt ggc gat gcc ttt ttg att gag act gct cag gac ttg ctt cag 643

Gly Gly Gly Asp Ala Phe Leu Ile Glu Thr Ala Gln Asp Leu Leu Gln

170 175 180

gtc aag gct gcg gtt cac ggc gtt caa gat gcc atg gct gaa ctt gat 69l

Val Lys Ala Ala Val His Gly Val Gln Asp Ala Met Ala Glu Leu Asp

185 190 195

aca ttc ttg ccc att att tgc cac gtc acc gta gag acc acc ggc acc 739

Thr Phe Leu Pro Ile Ile Cys His Val Thr Val Glu Thr Thr Gly Thr

200 205 210

atg ctc atg ggt tct gag atc ggt gcc gcg ttg aca gcg ctg cag cca 787

Met Leu Met Gly Ser Glu Ile Gly Ala Ala Leu Thr Ala Leu Gln Pro

215 220 225

ctg ggt atc gac atg att ggt ctg aac tgc gcc acc ggc cca gat gag 835

Leu Gly Ile Asp Met Ile Gly Leu Asn Cys Ala Thr Gly Pro Asp Glu

230 235 240 245

atg agc gag cac ctg cgt tac ctg tcc aag cac gcc gat att cct gtg 883

Met Ser Glu His Leu Arg Tyr Leu Ser Lys His Ala Asp Ile Pro Val

250 255 260

tcg gtg atg cct aac gca ggt ctt cct gtc ctg ggt aaa aac ggt gca 931

Ser Val Met Pro Asn Ala Gly Leu Pro Val Leu Gly Lys Asn Gly Ala

265 270 275

gaa tac cca ctt gag gct gag gat ttg gcg cag gcg ctg gct gga ttc 979

Glu Tyr Pro Leu Glu Ala Glu Asp Leu Ala Gln Ala Leu Ala Gly Phe

280 285 290

gtc tcc gaa tat ggc ctg tcc atg gtg ggt ggt tgt tgt ggc acc aca 1027

Val Ser Glu Tyr Gly Leu Ser Met Val Gly Gly Cys Cys Gly Thr Thr

295 300 305

cct gag cac atc cgt gcg gtc cgc gat gcg gtg gtt ggt gtt cca gag 1075

Pro Glu His Ile Arg Ala Val Arg Asp Ala Val Val Gly Val Pro Glu

310 315 320 325

cag gaa acc tcc aca ctg acc aag atc cct gca ggc cct gtt gag cag 1123

Gln Glu Thr Ser Thr Leu Thr Lys Ile Pro Ala Gly Pro Val Glu Gln

330 335 340

gcc tcc cgc gag gtg gag aaa gag gac tcc gtc gcg tcg ctg tac acc 1171

Ala Ser Arg Glu Val Glu Lys Glu Asp Ser Val Ala Ser Leu Tyr Thr

345 350 355

tcg gtg cca ttg tcc cag gaa acc ggc att tcc atg atc ggt gag cgc 1219

Ser Val Pro Leu Ser Gln Glu Thr Gly Ile Ser Met Ile Gly Glu Arg

360 365 370

acc aac tcc aac ggt tcc aag gca ttc cgt gag gca atg ctg tct ggc 1267

Thr Asn Ser Asn Gly Ser Lys Ala Phe Arg Glu Ala Met Leu Ser Gly

375 380 385

gat tgg gaa aag tgt gtg gat att gcc aag cag caa acc cgc gat ggt 1315

Asp Trp Glu Lys Cys Val Asp Ile Ala Lys Gln Gln Thr Arg Asp Gly

390 395 400 405

gca cac atg ctg gat ctt tgt gtg gat tac gtg gga cga gac ggc acc 1363

Ala His Met Leu Asp Leu Cys Val Asp Tyr Val Gly Arg Asp Gly Thr

410 415 420

gcc gat atg gcg acc ttg gca gca ctt ctt gct acc agc tcc act ttg 1411

Ala Asp Met Ala Thr Leu Ala Ala Leu Leu Ala Thr Ser Ser Thr Leu

425 430 435

cca atc atg att gac tcc acc gag cca gag gtt att cgc aca ggc ctt 1459

Pro Ile Met Ile Asp Ser Thr Glu Pro Glu Val Ile Arg Thr Gly Leu

440 445 450

gag cac ttg ggt gga cga agc atc gtt aac tcc gtc aac ttt gaa gac 1507

Glu His Leu Gly Gly Arg Ser Ile Val Asn Ser Val Asn Phe Glu Asp

455 460 465

ggc gat ggc cct gag tcc cgc tac cag cgc atc atg aaa ctg gta aag 1555

Gly Asp Gly Pro Glu Ser Arg Tyr Gln Arg Ile Met Lys Leu Val Lys

470 475 480 485

cag cac ggt gcg gcc gtg gtt gcg ctg acc att gat gag gaa ggc cag 1603

Gln His Gly Ala Ala Val Val Ala Leu Thr Ile Asp Glu Glu Gly Gln

490 495 500

gca cgt acc gct gag cac aag gtg cgc att gct aaa cga ctg att gac 1651

Ala Arg Thr Ala Glu His Lys Val Arg Ile Ala Lys Arg Leu Ile Asp

505 510 515

gat atc acc ggc agc tac ggc ctg gat atc aaa gac atc gtt gtg gac 1699

Asp Ile Thr Gly Ser Tyr Gly Leu Asp Ile Lys Asp Ile Val Val Asp

520 525 530

tgc ctg acc ttc ccg atc tct act ggc cag gaa gaa acc agg cga gat 1747

Cys Leu Thr Phe Pro Ile Ser Thr Gly Gln Glu Glu Thr Arg Arg Asp

535 540 545

ggc att gaa acc atc gaa gcc atc cgc gag ctg aag aag ctc tac cca 1795

Gly Ile Glu Thr Ile Glu Ala Ile Arg Glu Leu Lys Lys Leu Tyr Pro

550 555 560 565

gaa atc cac acc acc ctg ggt ctg tcc aat att tcc ttc ggc ctg aac 1843

Glu Ile His Thr Thr Leu Gly Leu Ser Asn Ile Ser Phe Gly Leu Asn

570 575 580

cct gct gca cgc cag gtt ctt aac tct gtg ttc ctc aat gag tgc att 1891

Pro Ala Ala Arg Gln Val Leu Asn Ser Val Phe Leu Asn Glu Cys Ile

585 590 595

gag gct ggt ctg gac tct gcg att gcg cac agc tcc aag att ttg ccg 1939

Glu Ala Gly Leu Asp Ser Ala Ile Ala His Ser Ser Lys Ile Leu Pro

600 605 610

atg aac cgc att gat gat cgc cag cgc gaa gtg gcg ttg gat atg gtc 1987

Met Asn Arg Ile Asp Asp Arg Gln Arg Glu Val Ala Leu Asp Met Val

615 620 625

tat gat cgc cgc acc gag gat tac gat ccg ctg cag gaa ttc atg cag 2035

Tyr Asp Arg Arg Thr Glu Asp Tyr Asp Pro Leu Gln Glu Phe Met Gln

630 635 640 645

ctg ttt gag ggc gtt tct gct gcc gat gcc aag gat gct cgc gct gaa 2083

Leu Phe Glu Gly Val Ser Ala Ala Asp Ala Lys Asp Ala Arg Ala Glu

650 655 660

cag ctg gcc gct atg cct ttg ttt gag cgt ttg gca cag cgc atc atc 2131

Gln Leu Ala Ala Met Pro Leu Phe Glu Arg Leu Ala Gln Arg Ile Ile

665 670 675

gac ggc gat aag aat ggc ctt gag gat gat ctg gaa gca ggc atg aag 2179

Asp Gly Asp Lys Asn Gly Leu Glu Asp Asp Leu Glu Ala Gly Met Lys

680 685 690

gag aag tct cct att gcg atc atc aac gag gac ctt ctc aac ggc atg 2227

Glu Lys Ser Pro Ile Ala Ile Ile Asn Glu Asp Leu Leu Asn Gly Met

695 700 705

aag acc gtg ggt gag ctg ttt ggt tcc gga cag atg cag ctg cca ttc 2275

Lys Thr Val Gly Glu Leu Phe Gly Ser Gly Gln Met Gln Leu Pro Phe

710 715 720 725

gtg ctg caa tcg gca gaa acc atg aaa act gcg gtg gcc tat ttg gaa 2323

Val Leu Gln Ser Ala Glu Thr Met Lys Thr Ala Val Ala Tyr Leu Glu

730 735 740

ccg ttc atg gaa gag gaa gca gaa gct acc gga tct gcg cag gca gag 2371

Pro Phe Met Glu Glu Glu Ala Glu Ala Thr Gly Ser Ala Gln Ala Glu

745 750 755

ggc aag ggc aaa atc gtc gtg gcc acc gtc aag ggt gac gtg cac gat 2419

Gly Lys Gly Lys Ile Val Val Ala Thr Val Lys Gly Asp Val His Asp

760 765 770

atc ggc aag aac ttg gtg gac atc att ttg tcc aac aac ggt tac gac 2467

Ile Gly Lys Asn Leu Val Asp Ile Ile Leu Ser Asn Asn Gly Tyr Asp

775 780 785

gtg gtg aac ttg ggc atc aag cag cca ctg tcc gcc atg ttg gaa gca 2515

Val Val Asn Leu Gly Ile Lys Gln Pro Leu Ser Ala Met Leu Glu Ala

790 795 800 805

gcg gaa gaa cac aaa gca gac gtc atc ggc atg tcg gga ctt ctt gtg 2563

Ala Glu Glu His Lys Ala Asp Val Ile Gly Met Ser Gly Leu Leu Val

810 815 820

aag tcc acc gtg gtg atg aag gaa aac ctt gag gag atg aac aac gcc 2611

Lys Ser Thr Val Val Met Lys Glu Asn Leu Glu Glu Met Asn Asn Ala

825 830 835

ggc gca tcc aat tac cca gtc att ttg ggt ggc gct gcg ctg acg cgt 2659

Gly Ala Ser Asn Tyr Pro Val Ile Leu Gly Gly Ala Ala Leu Thr Arg

840 845 850

acc tac gtg gaa aac gat ctc aac gag gtg tac acc ggt gag gtg tac 2707

Thr Tyr Val Glu Asn Asp Leu Asn Glu Val Tyr Thr Gly Glu Val Tyr

855 860 865

tac gcc cgt gat gct ttc gag ggc ctg cgc ctg atg gat gag gtg atg 2755

Tyr Ala Arg Asp Ala Phe Glu Gly Leu Arg Leu Met Asp Glu Val Met

870 875 880 885

gca gaa aag cgt ggt gaa gga ctt gat ccc aac tca cca gaa gct att 2803

Ala Glu Lys Arg Gly Glu Gly Leu Asp Pro Asn Ser Pro Glu Ala Ile

890 895 900

gag cag gcg aag aag aag gcg gaa cgt aag gct cgt aat gag cgt tcc 2851

Glu Gln Ala Lys Lys Lys Ala Glu Arg Lys Ala Arg Asn Glu Arg Ser

905 910 915

cgc aag att gcc gcg gag cgt aaa gct aat gcg gct ccc gtg att gtt 2899

Arg Lys Ile Ala Ala Glu Arg Lys Ala Asn Ala Ala Pro Val Ile Val

920 925 930

ccg gag cgt tct gat gtc tcc acc gat act cca acc gcg gca cca ccg 2947

Pro Glu Arg Ser Asp Val Ser Thr Asp Thr Pro Thr Ala Ala Pro Pro

935 940 945

ttc tgg gga acc cgc att gtc aag ggt ctg ccc ttg gcg gag ttc ttg 2995

Phe Trp Gly Thr Arg Ile Val Lys Gly Leu Pro Leu Ala Glu Phe Leu

950 955 960 965

ggc aac ctt gat gag cgc gcc ttg ttc atg ggg cag tgg ggt ctg aaa 3043

Gly Asn Leu Asp Glu Arg Ala Leu Phe Met Gly Gln Trp Gly Leu Lys

970 975 980

tcc acc cgc ggc aac gag ggt cca agc tat gag gat ttg gtg gaa act 3091

Ser Thr Arg Gly Asn Glu Gly Pro Ser Tyr Glu Asp Leu Val Glu Thr

985 990 995

gaa ggc cga cca cgc ctg cgc tac tgg ctg gat cgc ctg aag tct gag 3139

Glu Gly Arg Pro Arg Leu Arg Tyr Trp Leu Asp Arg Leu Lys Ser Glu

1000 1005 1010

ggc att ttg gac cac gtg gcc ttg gtg tat ggc tac ttc cca gcg gtc 3187

Gly Ile Leu Asp His Val Ala Leu Val Tyr Gly Tyr Phe Pro Ala Val

1015 1020 1025

gcg gaa ggc gat gac gtg gtg atc ttg gaa tcc ccg gat cca cac gca 3235

Ala Glu Gly Asp Asp Val Val Ile Leu Glu Ser Pro Asp Pro His Ala

1030 1035 1040 1045

gcc gaa cgc atg cgc ttt agc ttc cca cgc cag cag cgc ggc agg ttc 3283

Ala Glu Arg Met Arg Phe Ser Phe Pro Arg Gln Gln Arg Gly Arg Phe

1050 1055 1060

ttg tgc atc gcg gat ttc att cgc cca cgc gag caa gct gtc aag gac 3331

Leu Cys Ile Ala Asp Phe Ile Arg Pro Arg Glu Gln Ala Val Lys Asp

1065 1070 1075

ggc caa gtg gac gtc atg cca ttc cag ctg gtc acc atg ggt aat cct 3379

Gly Gln Val Asp Val Met Pro Phe Gln Leu Val Thr Met Gly Asn Pro

1080 1085 1090

att gct gat ttc gcc aac gag ttg ttc gca gcc aat gaa tac cgc gag 3427

Ile Ala Asp Phe Ala Asn Glu Leu Phe Ala Ala Asn Glu Tyr Arg Glu

1095 1100 1105

tac ttg gaa gtt cac ggc atc ggc gtg cag ctc acc gaa gca ttg gcc 3475

Tyr Leu Glu Val His Gly Ile Gly Val Gln Leu Thr Glu Ala Leu Ala

1110 1115 1120 1125

gag tac tgg cac tcc cga gtg cgc agc gaa ctc aag ctg aac gac ggt 3523

Glu Tyr Trp His Ser Arg Val Arg Ser Glu Leu Lys Leu Asn Asp Gly

1130 1135 1140

gga tct gtc gct gat ttt gat cca gaa gac aag acc aag ttc ttc gac 3571

Gly Ser Val Ala Asp Phe Asp Pro Glu Asp Lys Thr Lys Phe Phe Asp

1145 1150 1155

ctg gat tac cgc ggc gcc cgc ttc tcc ttt ggt tac ggt tct tgc cct 3619

Leu Asp Tyr Arg Gly Ala Arg Phe Ser Phe Gly Tyr Gly Ser Cys Pro

1160 1165 1170

gat ctg gaa gac cgc gca aag ctg gtg gaa ttg ctc gag cca ggc cgt 3667

Asp Leu Glu Asp Arg Ala Lys Leu Val Glu Leu Leu Glu Pro Gly Arg

1175 1180 1185

atc ggc gtg gag ttg tcc gag gaa ctc cag ctg cac cca gag cag tcc 3715

Ile Gly Val Glu Leu Ser Glu Glu Leu Gln Leu His Pro Glu Gln Ser

1190 1195 1200 1205

aca gac gcg ttt gtg ctc tac cac cca gag gca aag tac ttt aac gtc 3763

Thr Asp Ala Phe Val Leu Tyr His Pro Glu Ala Lys Tyr Phe Asn Val

1210 1215 1220

taacaccttt gagagggaaa actttcccgc 3793

<210>156

<211>1221

<212>PRT

<213>谷氨酸棒状杆菌

<400>156

Met Ser Thr Ser Val Thr Ser Pro Ala His Asn Asn Ala His Ser Ser

1 5 10 15

Glu Phe Leu Asp Ala Leu Ala Asn His Val Leu Ile Gly Asp Gly Ala

20 25 30

Met Gly Thr Gln Leu Gln Gly Phe Asp Leu Asp Val Glu Lys Asp Phe

35 40 45

Leu Asp Leu Glu Gly Cys Asn Glu Ile Leu Asn Asp Thr Arg Pro Asp

50 55 60

Val Leu Arg Gln Ile His Arg Ala Tyr Phe Glu Ala Gly Ala Asp Leu

65 70 75 80

Val Glu Thr Asn Thr Phe Gly Cys Asn Leu Pro Asn Leu Ala Asp Tyr

85 90 95

Asp Ile Ala Asp Arg Cys Arg Glu Leu Ala Tyr Lys Gly Thr Ala Val

100 105 110

Ala Arg Glu Val Ala Asp Glu Met Gly Pro Gly Arg Asn Gly Met Arg

115 120 125

Arg Phe Val Val Gly Ser Leu Gly Pro Gly Thr Lys Leu Pro Ser Leu

130 135 140

Gly His Ala Pro Tyr Ala Asp Leu Arg Gly His Tyr Lys Glu Ala Ala

145 150 155 160

Leu Gly Ile Ile Asp Gly Gly Gly Asp Ala Phe Leu Ile Glu Thr Ala

165 170 175

Gln Asp Leu Leu Gln Val Lys Ala Ala Val His Gly Val Gln Asp Ala

180 185 190

Met Ala Glu Leu Asp Thr Phe Leu Pro Ile Ile Cys His Val Thr Val

195 200 205

Glu Thr Thr Gly Thr Met Leu Met Gly Ser Glu Ile Gly Ala Ala Leu

210 215 220

Thr Ala Leu Gln Pro Leu Gly Ile Asp Met Ile Gly Leu Asn Cys Ala

225 230 235 240

Thr Gly Pro Asp Glu Met Ser Glu His Leu Arg Tyr Leu Ser Lys His

245 250 255

Ala Asp Ile Pro Val Ser Val Met Pro Asn Ala Gly Leu Pro Val Leu

260 265 270

Gly Lys Asn Gly Ala Glu Tyr Pro Leu Glu Ala Glu Asp Leu Ala Gln

275 280 285

Ala Leu Ala Gly Phe Val Ser Glu Tyr Gly Leu Ser Met Val Gly Gly

290 295 300

Cys Cys Gly Thr Thr Pro Glu His Ile Arg Ala Val Arg Asp Ala Val

305 310 315 320

Val Gly Val Pro Glu Gln Glu Thr Ser Thr Leu Thr Lys Ile Pro Ala

325 330 335

Gly Pro Val Glu Gln Ala Ser Arg Glu Val Glu Lys Glu Asp Ser Val

340 345 350

Ala Ser Leu Tyr Thr Ser Val Pro Leu Ser Gln Glu Thr Gly Ile Ser

355 360 365

Met Ile Gly Glu Arg Thr Asn Ser Asn Gly Ser Lys Ala Phe Arg Glu

370 375 380

Ala Met Leu Ser Gly Asp Trp Glu Lys Cys Val Asp Ile Ala Lys Gln

385 390 395 400

Gln Thr Arg Asp Gly Ala His Met Leu Asp Leu Cys Val Asp Tyr Val

405 410 415

Gly Arg Asp Gly Thr Ala Asp Met Ala Thr Leu Ala Ala Leu Leu Ala

420 425 430

Thr Ser Ser Thr Leu Pro Ile Met Ile Asp Ser Thr Glu Pro Glu Val

435 440 445

Ile Arg Thr Gly Leu Glu His Leu Gly Gly Arg Ser Ile Val Asn Ser

450 455 460

Val Asn Phe Glu Asp Gly Asp Gly Pro Glu Ser Arg Tyr Gln Arg Ile

465 470 475 480

Met Lys Leu Val Lys Gln His Gly Ala Ala Val Val Ala Leu Thr Ile

485 490 495

Asp Glu Glu Gly Gln Ala Arg Thr Ala Glu His Lys Val Arg Ile Ala

500 505 510

Lys Arg Leu Ile Asp Asp Ile Thr Gly Ser Tyr Gly Leu Asp Ile Lys

515 520 525

Asp Ile Val Val Asp Cys Leu Thr Phe Pro Ile Ser Thr Gly Gln Glu

530 535 540

Glu Thr Arg Arg Asp Gly Ile Glu Thr Ile Glu Ala Ile Arg Glu Leu

545 550 555 560

Lys Lys Leu Tyr Pro Glu Ile His Thr Thr Leu Gly Leu Ser Asn Ile

565 570 575

Ser Phe Gly Leu Asn Pro Ala Ala Arg Gln Val Leu Asn Ser Val Phe

580 585 590

Leu Asn Glu Cys Ile Glu Ala Gly Leu Asp Ser Ala Ile Ala His Ser

595 600 605

Ser Lys Ile Leu Pro Met Asn Arg Ile Asp Asp Arg Gln Arg Glu Val

610 615 620

Ala Leu Asp Met Val Tyr Asp Arg Arg Thr Glu Asp Tyr Asp Pro Leu

625 630 635 640

Gln Glu Phe Met Gln Leu Phe Glu Gly Val Ser Ala Ala Asp Ala Lys

645 650 655

Asp Ala Arg Ala Glu Gln Leu Ala Ala Met Pro Leu Phe Glu Arg Leu

660 665 670

Ala Gln Arg Ile Ile Asp Gly Asp Lys Asn Gly Leu Glu Asp Asp Leu

675 680 685

Glu Ala Gly Met Lys Glu Lys Ser Pro Ile Ala Ile Ile Asn Glu Asp

690 695 700

Leu Leu Asn Gly Met Lys Thr Val Gly Glu Leu Phe Gly Ser Gly Gln

705 710 715 720

Met Gln Leu Pro Phe Val Leu Gln Ser Ala Glu Thr Met Lys Thr Ala

725 730 735

Val Ala Tyr Leu Glu Pro Phe Met Glu Glu Glu Ala Glu Ala Thr Gly

740 745 750

Ser Ala Gln Ala Glu Gly Lys Gly Lys Ile Val Val Ala Thr Val Lys

755 760 765

Gly Asp Val His Asp Ile Gly Lys Asn Leu Val Asp Ile Ile Leu Ser

770 775 780

Asn Asn Gly Tyr Asp Val Val Asn Leu Gly Ile Lys Gln Pro Leu Ser

785 790 795 800

Ala Met Leu Glu Ala Ala Glu Glu His Lys Ala Asp Val Ile Gly Met

805 810 815

Ser Gly Leu Leu Val Lys Ser Thr Val Val Met Lys Glu Asn Leu Glu

820 825 830

Glu Met Asn Asn Ala Gly Ala Ser Asn Tyr Pro Val Ile Leu Gly Gly

835 840 845

Ala Ala Leu Thr Arg Thr Tyr Val Glu Asn Asp Leu Asn Glu Val Tyr

850 855 860

Thr Gly Glu Val Tyr Tyr Ala Arg Asp Ala Phe Glu Gly Leu Arg Leu

865 870 875 880

Met Asp Glu Val Met Ala Glu Lys Arg Gly Glu Gly Leu Asp Pro Asn

885 890 895

Ser Pro Glu Ala Ile Glu Gln Ala Lys Lys Lys Ala Glu Arg Lys Ala

900 905 910

Arg Asn Glu Arg Ser Arg Lys Ile Ala Ala Glu Arg Lys Ala Asn Ala

915 920 925

Ala Pro Val Ile Val Pro Glu Arg Ser Asp Val Ser Thr Asp Thr Pro

930 935 940

Thr Ala Ala Pro Pro Phe Trp Gly Thr Arg Ile Val Lys Gly Leu Pro

945 950 955 960

Leu Ala Glu Phe Leu Gly Asn Leu Asp Glu Arg Ala Leu Phe Met Gly

965 970 975

Gln Trp Gly Leu Lys Ser Thr Arg Gly Asn Glu Gly Pro Ser Tyr Glu

980 985 990

Asp Leu Val Glu Thr Glu Gly Arg Pro Arg Leu Arg Tyr Trp Leu Asp

995 1000 1005

Arg Leu Lys Ser Glu Gly Ile Leu Asp His Val Ala Leu Val Tyr Gly

1010 1015 1020

Tyr Phe Pro Ala Val Ala Glu Gly Asp Asp Val Val Ile Leu Glu Ser

1025 1030 1035 1040

Pro Asp Pro His Ala Ala Glu Arg Met Arg Phe Ser Phe Pro Arg Gln

1045 1050 1055

Gln Arg Gly Arg Phe Leu Cys Ile Ala Asp Phe Ile Arg Pro Arg Glu

1060 1065 1070

Gln Ala Val Lys Asp Gly Gln Val Asp Val Met Pro Phe Gln Leu Val

1075 1080 1085

Thr Met Gly Asn Pro Ile Ala Asp Phe Ala Asn Glu Leu Phe Ala Ala

1090 1095 1100

Asn Glu Tyr Arg Glu Tyr Leu Glu Val His Gly Ile Gly Val Gln Leu

1105 1110 1115 1120

Thr Glu Ala Leu Ala Glu Tyr Trp His Ser Arg Val Arg Ser Glu Leu

1125 1130 1135

Lys Leu Asn Asp Gly Gly Ser Val Ala Asp Phe Asp Pro Glu Asp Lys

1140 1145 1150

Thr Lys Phe Phe Asp Leu Asp Tyr Arg Gly Ala Arg Phe Ser Phe Gly

1155 1160 1165

Tyr Gly Ser Cys Pro Asp Leu Glu Asp Arg Ala Lys Leu Val Glu Leu

1170 1175 1180

Leu Glu Pro Gly Arg Ile Gly Val Glu Leu Ser Glu Glu Leu Gln Leu

1185 1190 1195 1200

His Pro Glu Gln Ser Thr Asp Ala Phe Val Leu Tyr His Pro Glu Ala

1205 1210 1215

Lys Tyr Phe Asn Val

1220

<210>157

<211>1468

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1438)

<223>RXA04228

<400>157

tttccacacg cgttgcggat tttctccgca gtggaaaaac tcactcgccc aggctgcgaa 60

aacgcccgcg acacagtgga aggggagacg ccagcgactt ttg cga cat cat aaa 115

Leu Arg His His Lys

1 5

tgg tgg ctt ttg agt cgc tgt gag ccc cag aat ctg tca tgc aca aga 163

Trp Trp Leu Leu Ser Arg Cys Glu Pro Gln Asn Leu Ser Cys Thr Arg

10 15 20

gta tat agc gca aaa gaa atc act agt ctt gat tct atg ttg acg atg 211

Val Tyr Ser Ala Lys Glu Ile Thr Ser Leu Asp Ser Met Leu Thr Met

25 30 35

ccg ata ccc gag tac ctg cac gaa att tta gat gat gtc cgc gac acc 259

Pro Ile Pro Glu Tyr Leu His Glu Ile Leu Asp Asp Val Arg Asp Thr

40 45 50

acc tcc ggc gag ttg gcc gat tac atc ccg gaa cta aaa tct gcg gac 307

Thr Ser Gly Glu Leu Ala Asp Tyr Ile Pro Glu Leu Lys Ser Ala Asp

55 60 65

cca aac ccg ctg gca gta gcc ctg tgc acc gtt aac gga cac atc tac 355

Pro Asn Pro Leu Ala Val Ala Leu Cys Thr Val Asn Gly His Ile Tyr

70 75 80 85

agc gca ggc gat gac gac atc gaa ttc acc atg caa agt att tcc aag 403

Ser Ala Gly Asp Asp Asp Ile Glu Phe Thr Met Gln Ser Ile Ser Lys

90 95 100

cca ttt gcc tac gca ctc gca ctc caa gaa tgc ggc ttt gat gag gtc 451

Pro Phe Ala Tyr Ala Leu Ala Leu Gln Glu Cys Gly Phe Asp Glu Val

105 110 115

tct gca tcc gtg gcc ttg gag ccc tcc ggt gag gcc ttc aac gaa ctt 499

Ser Ala Ser Val Ala Leu Glu Pro Ser Gly Glu Ala Phe Asn Glu Leu

120 125 130

tcc ctc gac ggc gaa aac cgc ccc atg aac ccc atg atc aac gcc ggc 547

Ser Leu Asp Gly Glu Asn Arg Pro Met Asn Pro Met Ile Asn Ala Gly

135 140 145

gcg atc gcc atc aac cag ctg atc aac ggc tcc gat tcc acc gtg gaa 595

Ala Ile Ala Ile Asn Gln Leu Ile Asn Gly Ser Asp Ser Thr Val Glu

150 155 160 165

gac cgc gtg gaa aaa atc cga cac tac ttc tct gaa ctt gct gga cgc 643

Asp Arg Val Glu Lys Ile Arg His Tyr Phe Ser Glu Leu Ala Gly Arg

170 175 180

gaa ctc acc atc gac cgc gtg ctt gcc gaa tcc gaa ctc gcc ggc gcc 691

Glu Leu Thr Ile Asp Arg Val Leu Ala Glu Ser Glu Leu Ala Gly Ala

185 190 195

gac cgc aac ctc tcc atc gcc cac atg ctg cgc aat tac ggc gtc atc 739

Asp Arg Asn Leu Ser Ile Ala His Met Leu Arg Asn Tyr Gly Val Ile

200 205 210

gaa gac gaa gcc cac gac gcc gtc ctc agc tac acg ctg caa tgc gcc 787

Glu Asp Glu Ala His Asp Ala Val Leu Ser Tyr Thr Leu Gln Cys Ala

215 220 225

atc aaa gta acc acg cgc gac ctc gca gtc atg acc gcc acg ctc gcc 835

Ile Lys Val Thr Thr Arg Asp Leu Ala Val Met Thr Ala Thr Leu Ala

230 235 240 245

gcc ggc ggc aca cac cca att acc ggc aag aag ctt ctc gac gcc cgc 883

Ala Gly Gly Thr His Pro Ile Thr Gly Lys Lys Leu Leu Asp Ala Arg

250 255 260

gtc tgc cgc ctc acc ctc tcc gtc atg gct tca gca ggc atg tac gac 931

Val Cys Arg Leu Thr Leu Ser Val Met Ala Ser Ala Gly Met Tyr Asp

265 270 275

gag gca ggg cag tgg ctc tcc acc gta ggc atc ccc gcg aaa tca gga 979

Glu Ala Gly Gln Trp Leu Ser Thr Val Gly Ile Pro Ala Lys Ser Gly

280 285 290

gtc gcc ggc gga ctc atc ggc att ctg cca ggt cag ctg ggc atc gcc 1027

Val Ala Gly Gly Leu Ile Gly Ile Leu Pro Gly Gln Leu Gly Ile Ala

295 300 305

aca ttt tcc cca cgc ctg aac ccc aaa ggc aac agc gtg cgc ggc gta 1075

Thr Phe Ser Pro Arg Leu Asn Pro Lys Gly Asn Ser Val Arg Gly Val

310 315 320 325

aaa ata ttc aaa cag ctt tcc gac gac atg ggc ctc cac ctc atg tcc 1123

Lys Ile Phe Lys Gln Leu Ser Asp Asp Met Gly Leu His Leu Met Ser

330 335 340

acc gag cag gta tcc ggc cac gca gta cga tcc atc acg cgg gac ggc 117l

Thr Glu Gln Val Ser Gly His Ala Val Arg Ser Ile Thr Arg Asp Gly

345 350 355

gac acc acc ttc atc caa atg cag ggc gcc atg aac ttc tcc gcc agc 1219

Asp Thr Thr Phe Ile Gln Met Gln Gly Ala Met Asn Phe Ser Ala Ser

360 365 370

gaa agc ttc ctc cac gcc atc gtg gaa cac aac ttt gaa ggc acc gaa 1267

Glu Ser Phe Leu His Ala Ile Val Glu His Asn Phe Glu Gly Thr Glu

375 380 385

gtt gtt ctt gat ctc acc cga gta ctt agc ttc cac ccc gta gcc atc 1315

Val Val Leu Asp Leu Thr Arg Val Leu Ser Phe His Pro Val Ala Ile

390 395 400 405

cgc atg atc aaa gaa ggc ctc aaa cgc atc cgc gac gca ggc ttt gag 1363

Arg Met Ile Lys Glu Gly Leu Lys Arg Ile Arg Asp Ala Gly Phe Glu

410 415 420

gtg ttc atc ctc gac cca gat gac gta ctg ccc gat ttc atg ttt tcc 141l

Val Phe Ile Leu Asp Pro Asp Asp Val Leu Pro Asp Phe Met Phe Ser

425 430 435

gac ggc acc atc tgc aaa gaa cga gtg tgaccggtag ctttatgggc 1458

Asp Gly Thr Ile Cys Lys Glu Arg Val

440 445

tgaacaattc 1468

<210>158

<211>446

<212>PRT

<213>谷氨酸棒状杆菌

<400>158

Leu Arg His His Lys Trp Trp Leu Leu Ser Arg Cys Glu Pro Gln Asn

1 5 10 15

Leu Ser Cys Thr Arg Val Tyr Ser Ala Lys Glu Ile Thr Ser Leu Asp

20 25 30

Ser Met Leu Thr Met Pro Ile Pro Glu Tyr Leu His Glu Ile Leu Asp

35 40 45

Asp Val Arg Asp Thr Thr Ser Gly Glu Leu Ala Asp Tyr Ile Pro Glu

50 55 60

Leu Lys Ser Ala Asp Pro Asn Pro Leu Ala Val Ala Leu Cys Thr Val

65 70 75 80

Asn Gly His Ile Tyr Ser Ala Gly Asp Asp Asp Ile Glu Phe Thr Met

85 90 95

Gln Ser Ile Ser Lys Pro Phe Ala Tyr Ala Leu Ala Leu Gln Glu Cys

100 105 110

Gly Phe Asp Glu Val Ser Ala Ser Val Ala Leu Glu Pro Ser Gly Glu

115 120 125

Ala Phe Asn Glu Leu Ser Leu Asp Gly Glu Asn Arg Pro Met Asn Pro

130 135 140

Met Ile Asn Ala Gly Ala Ile Ala Ile Asn Gln Leu Ile Asn Gly Ser

145 150 155 160

Asp Ser Thr Val Glu Asp Arg Val Glu Lys Ile Arg His Tyr Phe Ser

165 170 175

Glu Leu Ala Gly Arg Glu Leu Thr Ile Asp Arg Val Leu Ala Glu Ser

180 185 190

Glu Leu Ala Gly Ala Asp Arg Asn Leu Ser Ile Ala His Met Leu Arg

195 200 205

Asn Tyr Gly Val Ile Glu Asp Glu Ala His Asp Ala Val Leu Ser Tyr

210 215 220

Thr Leu Gln Cys Ala Ile Lys Val Thr Thr Arg Asp Leu Ala Val Met

225 230 235 240

Thr Ala Thr Leu Ala Ala Gly Gly Thr His Pro Ile Thr Gly Lys Lys

245 250 255

Leu Leu Asp Ala Arg Val Cys Arg Leu Thr Leu Ser Val Met Ala Ser

260 265 270

Ala Gly Met Tyr Asp Glu Ala Gly Gln Trp Leu Ser Thr Val Gly Ile

275 280 285

Pro Ala Lys Ser Gly Val Ala Gly Gly Leu Ile Gly Ile Leu Pro Gly

290 295 300

Gln Leu Gly Ile Ala Thr Phe Ser Pro Arg Leu Asn Pro Lys Gly Asn

305 310 315 320

Ser Val Arg Gly Val Lys Ile Phe Lys Gln Leu Ser Asp Asp Met Gly

325 330 335

Leu His Leu Met Ser Thr Glu Gln Val Ser Gly His Ala Val Arg Ser

340 345 350

Ile Thr Arg Asp Gly Asp Thr Thr Phe Ile Gln Met Gln Gly Ala Met

355 360 365

Asn Phe Ser Ala Ser Glu Ser Phe Leu His Ala Ile Val Glu His Asn

370 375 380

Phe Glu Gly Thr Glu Val Val Leu Asp Leu Thr Arg Val Leu Ser Phe

385 390 395 400

His Pro Val Ala Ile Arg Met Ile Lys Glu Gly Leu Lys Arg Ile Arg

405 410 415

Asp Ala Gly Phe Glu Val Phe Ile Leu Asp Pro Asp Asp Val Leu Pro

420 425 430

Asp Phe Met Phe Ser Asp Gly Thr Ile Cys Lys Glu Arg Val

435 440 445

<210>159

<211>1174

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1144)

<223>RXA04360

<400>159

cagtttcacc ctgagtcagt gctaagccca acgggtcctg tcattttgtc ccgctgtgtc 60

gaacagcttc tcgcgaacta ataaaaaaag gatttgattc atg act tct cca gca 115

Met Thr Ser Pro Ala

1 5

aca ctg aaa gtt ctc aac gcc tac ttg gat aac ccc act cca acc ctg 163

Thr Leu Lys Val Leu Asn Ala Tyr Leu Asp Asn Pro Thr Pro Thr Leu

10 15 20

gag gag gca att gag gtg ttc acc ccg ctg acc gtg ggt gaa tac gat 211

Glu Glu Ala Ile Glu Val Phe Thr Pro Leu Thr Val Gly Glu Tyr Asp

25 30 35

gac gtg cac atc gca gcg ctg ctt gcc acc atc cgt act cgc ggt gag 259

Asp Val His Ile Ala Ala Leu Leu Ala Thr Ile Arg Thr Arg Gly Glu

40 45 50

cag ttc gct gat att gcc ggc gct gcc aag gcg ttc ctc gcg gcg gct 307

Gln Phe Ala Asp Ile Ala Gly Ala Ala Lys Ala Phe Leu Ala Ala Ala

55 60 65

cgt ccg ttc ccg att act ggc gca ggt ttg cta gat tcc gct ggt act 355

Arg Pro Phe Pro Ile Thr Gly Ala Gly Leu Leu Asp Ser Ala Gly Thr

70 75 80 85

ggt ggc gac ggt gcc aac acc atc aac atc acc acc ggc gca tcc ctg 403

Gly Gly Asp Gly Ala Asn Thr Ile Asn Ile Thr Thr Gly Ala Ser Leu

90 95 100

atc gca gca tcc ggt gga gtg aag ctg gtt aag cac ggc aac cgt tcg 45l

Ile Ala Ala Ser Gly Gly Val Lys Leu Val Lys His Gly Asn Arg Ser

105 110 115

gtg agc tcc aag tcc ggc tcc gcc gat gtg ctg gaa gcg ctg aat att 499

Val Ser Ser Lys Ser Gly Ser Ala Asp Val Leu Glu Ala Leu Asn Ile

120 125 130

cct ttg ggc ctt gat gtg gat cgt gct gtg aag tgg ttc gaa gcg tcc 547

Pro Leu Gly Leu Asp Val Asp Arg Ala Val Lys Trp Phe Glu Ala Ser

135 140 145

aac ttc acc ttc ctg ttc gca cct gcg tac aac cct gcg att gcg cat 595

Asn Phe Thr Phe Leu Phe Ala Pro Ala Tyr Asn Pro Ala Ile Ala His

150 155 160 165

gtg cag ccg gtt cgc cag gcg ctg aaa ttc ccc acc atc ttc aac acg 643

Val Gln Pro Val Arg Gln Ala Leu Lys Phe Pro Thr Ile Phe Asn Thr

170 175 180

ctt gga cca ttg ctg tcc ccg gcg cgc ccg gag cgt cag atc atg ggc 691

Leu Gly Pro Leu Leu Ser Pro Ala Arg Pro Glu Arg Gln Ile Met Gly

185 190 195

gtg gcc aat gcc aat cat gga cag ctc atc gcc gag gtc ttc cgc gag 739

Val Ala Asn Ala Asn His Gly Gln Leu Ile Ala Glu Val Phe Arg Glu

200 205 210

ttg ggc cgt aca cgc gcg ctt gtt gtg cat ggc gca ggc acc gat gag 787

Leu Gly Arg Thr Arg Ala Leu Val Val His Gly Ala Gly Thr Asp Glu

215 220 225

atc gca gtc cac ggc acc acc ttg gtg tgg gag ctt aaa gaa gac ggc 835

Ile Ala Val His Gly Thr Thr Leu Val Trp Glu Leu Lys Glu Asp Gly

230 235 240 245

acc atc gag cat tac acc atc gag cct gag gac ctt ggc ctt ggc cgc 883

Thr Ile Glu His Tyr Thr Ile Glu Pro Glu Asp Leu Gly Leu Gly Arg

250 255 260

tac acc ctt gag gat ctc gta ggt ggc ctc ggc act gag aac gcc gaa 931

Tyr Thr Leu Glu Asp Leu Val Gly Gly Leu Gly Thr Glu Asn Ala Glu

265 270 275

gct atg cgc gct act ttc gcg ggc acc ggc cct gat gca cac cgt gat 979

Ala Met Arg Ala Thr Phe Ala Gly Thr Gly Pro Asp Ala His Arg Asp

280 285 290

gcg ttg gct gcg tcc gca ggt gcg atg ttc tac ctc aac ggc gat gtc 1027

Ala Leu Ala Ala Ser Ala Gly Ala Met Phe Tyr Leu Asn Gly Asp Val

295 300 305

gac tcc ttg aaa gat ggt gca caa aag gcg ctt tcc ttg ctt gcc gac 1075

Asp Ser Leu Lys Asp Gly Ala Gln Lys Ala Leu Ser Leu Leu Ala Asp

310 315 320 325

ggc acc acc cag gca tgg ttg gcc aag cac gaa gag atc gat tac tca 1123

Gly Thr Thr Gln Ala Trp Leu Ala Lys His Glu Glu Ile Asp Tyr Ser

330 335 340

gaa aag gag tct tcc aat gac tagtaataat ctgcccacag tgttggaaag 1174

Glu Lys Glu Ser Ser Asn Asp

345

<210>160

<211>348

<212>PRT

<213>谷氨酸棒状杆菌

<400>160

Met Thr Ser Pro Ala Thr Leu Lys Val Leu Asn Ala Tyr Leu Asp Asn

1 5 10 15

Pro Thr Pro Thr Leu Glu Glu Ala Ile Glu Val Phe Thr Pro Leu Thr

20 25 30

Val Gly Glu Tyr Asp Asp Val His Ile Ala Ala Leu Leu Ala Thr Ile

35 40 45

Arg Thr Arg Gly Glu Gln Phe Ala Asp Ile Ala Gly Ala Ala Lys Ala

50 55 60

Phe Leu Ala Ala Ala Arg Pro Phe Pro Ile Thr Gly Ala Gly Leu Leu

65 70 75 80

Asp Ser Ala Gly Thr Gly Gly Asp Gly Ala Asn Thr Ile Asn Ile Thr

85 90 95

Thr Gly Ala Ser Leu Ile Ala Ala Ser Gly Gly Val Lys Leu Val Lys

100 105 110

His Gly Asn Arg Ser Val Ser Ser Lys Ser Gly Ser Ala Asp Val Leu

115 120 125

Glu Ala Leu Asn Ile Pro Leu Gly Leu Asp Val Asp Arg Ala Val Lys

130 135 140

Trp Phe Glu Ala Ser Asn Phe Thr Phe Leu Phe Ala Pro Ala Tyr Asn

145 150 155 160

Pro Ala Ile Ala His Val Gln Pro Val Arg Gln Ala Leu Lys Phe Pro

165 170 175

Thr Ile Phe Asn Thr Leu Gly Pro Leu Leu Ser Pro Ala Arg Pro Glu

180 185 190

Arg Gln Ile Met Gly Val Ala Asn Ala Asn His Gly Gln Leu Ile Ala

195 200 205

Glu Val Phe Arg Glu Leu Gly Arg Thr Arg Ala Leu Val Val His Gly

210 215 220

Ala Gly Thr Asp Glu Ile Ala Val His Gly Thr Thr Leu Val Trp Glu

225 230 235 240

Leu Lys Glu Asp Gly Thr Ile Glu His Tyr Thr Ile Glu Pro Glu Asp

245 250 255

Leu Gly Leu Gly Arg Tyr Thr Leu Glu Asp Leu Val Gly Gly Leu Gly

260 265 270

Thr Glu Asn Ala Glu Ala Met Arg Ala Thr Phe Ala Gly Thr Gly Pro

275 280 285

Asp Ala His Arg Asp Ala Leu Ala Ala Ser Ala Gly Ala Met Phe Tyr

290 295 300

Leu Asn Gly Asp Val Asp Ser Leu Lys Asp Gly Ala Gln Lys Ala Leu

305 310 315 320

Ser Leu Leu Ala Asp Gly Thr Thr Gln Ala Trp Leu Ala Lys His Glu

325 330 335

Glu Ile Asp Tyr Ser Glu Lys Glu Ser Ser Asn Asp

340 345

<210>161

<211>4530

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(1)..(4530)

<223>RXA07000

<400>161

atg aaa cca caa gga ctc tac asc cct gcg cat gaa cat gac gcc tgc 48

Met Lys Pro Gln Gly Leu Tyr Asn Pro Ala His Glu His Asp Ala Cys

1 5 10 15

ggt gtg gcg ttt att gcg gat atc cac ggt cga ccc agc cgc agc att 96

Gly Val Ala Phe Ile Ala Asp Ile His Gly Arg Pro Ser Arg Ser Ile

20 25 30

gtt gat cgt gca ctt gag gcg ctt cgc aac att gac cac cga ggt gcc 144

Val Asp Arg Ala Leu Glu Ala Leu Arg Asn Ile Asp His Arg Gly Ala

35 40 45

gcc ggt gca gag aag aac act ggc gat ggt gcg ggc atc ctc atg cag 192

Ala Gly Ala Glu Lys Asn Thr Gly Asp Gly Ala Gly Ile Leu Met Gln

50 55 60

att ccg gac ggc ttt tat cgt gaa gta tct ggc att gag ctt cct gag 240

Ile Pro Asp Gly Phe Tyr Arg Glu Val Ser Gly Ile Glu Leu Pro Glu

65 70 75 80

gca ggg gag tat gcc act ggt att gcg ttc ttg cct cgc ggt cgc atg 288

Ala Gly Glu Tyr Ala Thr Gly Ile Ala Phe Leu Pro Arg Gly Arg Met

85 90 95

gcg atg atg gat gct cag aag gaa att gag cgc atc gca aag caa gaa 336

Ala Met Met Asp Ala Gln Lys Glu Ile Glu Arg Ile Ala Lys Gln Glu

100 105 110

ggt gcc gat gtg ctt ggt tgg cgc atg gtt cct ttt gat tct cgt gat 384

Gly Ala Asp Val Leu Gly Trp Arg Met Val Pro Phe Asp Ser Arg Asp

115 120 125

ttg ggt tcc atg gct gag gag gcg atg cct agt ttc gcg cag att ttc 432

Leu Gly Ser Met Ala Glu Glu Ala Met Pro Ser Phe Ala Gln Ile Phe

130 135 140

ctt act gtg cct gga aaa tct ggt gaa gat ctt gac cgt gtg atg ttc 480

Leu Thr Val Pro Gly Lys Ser Gly Glu Asp Leu Asp Arg Val Met Phe

145 150 155 160

ttt atc cgt aag cgt tgt gag cgt gag ctg ggc acc acc aat ggt cgc 528

Phe Ile Arg Lys Arg Cys Glu Arg Glu Leu Gly Thr Thr Asn Gly Arg

165 170 175

gat acg gtg tat ttc ccg tcg cta tct tca cgc acc atc att tac aaa 576

Asp Thr Val Tyr Phe Pro Ser Leu Ser Ser Arg Thr Ile Ile Tyr Lys

180 185 190

ggc atg ttg acc act ctg cag ctt gag ggc ttc ttt gag gat ctg ggt 624

Gly Met Leu Thr Thr Leu Gln Leu Glu Gly Phe Phe Glu Asp Leu Gly

195 200 205

gat gct cgc ctg gag tcg gcc att gct att gtg cac tcg cgt ttc tcc 672

Asp Ala Arg Leu Glu Ser Ala Ile Ala Ile Val His Ser Arg Phe Ser

210 215 220

acg aac act ttc cca agc tgg ccg ctg gcg cac ccg tac cgt ttc gtt 720

Thr Asn Thr Phe Pro Ser Trp Pro Leu Ala His Pro Tyr Arg Phe Val

225 230 235 240

gcc cac aac ggt gag atc aac act gtg cgt ggc aat gaa aac tgg atg 768

Ala His Asn Gly Glu Ile Asn Thr Val Arg Gly Asn Glu Asn Trp Met

245 250 255

cgc gcc cgc gag gcg ctt atc aaa aac gac aag ctg ggc aat ttg agc 816

Arg Ala Arg Glu Ala Leu Ile Lys Asn Asp Lys Leu Gly Asn Leu Ser

260 265 270

agc gtg ctg cct atc tgc acc ccg gag ggc tcg gat acc gcg cgt ttc 864

Ser Val Leu Pro Ile Cys Thr Pro Glu Gly Ser Asp Thr Ala Arg Phe

275 280 285

gac gag gct ttg gag ctt ttg cac ctg ggc gga tac tca ctt ccg cat 912

Asp Glu Ala Leu Glu Leu Leu His Leu Gly Gly Tyr Ser Leu Pro His

290 295 300

gct gtt gcg atg atg atc cct cag gcg tgg gaa cac aac aag acg ctg 960

Ala Val Ala Met Met Ile Pro Gln Ala Trp Glu His Asn Lys Thr Leu

305 310 315 320

agc cct gag ctg cgt gat ttc tac gaa tac cac tct tgt ctg atg gag 1008

Ser Pro Glu Leu Arg Asp Phe Tyr Glu Tyr His Ser Cys Leu Met Glu

325 330 335

cca tgg gat ggt cct gca gcg ctg gca ttt act gac ggt cgt ttt gtg 1056

Pro Trp Asp Gly Pro Ala Ala Leu Ala Phe Thr Asp Gly Arg Phe Val

340 345 350

ggt gcc gtg ctg gac cgt aat ggc ctg cga cct ggg cga atc acc att 1104

Gly Ala Val Leu Asp Arg Asn Gly Leu Arg Pro Gly Arg Ile Thr Ile

355 360 365

act gat tcg ggt ttg gtt gtg atg gct tct gaa tcg gga gtg ttg gac 1152

Thr Asp Ser Gly Leu Val Val Met Ala Ser Glu Ser Gly Val Leu Asp

370 375 380

ttg agg gag gag agc gtc gta aag cgt act cgc gta cag cct gga cgc 1200

Leu Arg Glu Glu Ser Val Val Lys Arg Thr Arg Val Gln Pro Gly Arg

385 390 395 400

atg ttc ctt gtt gac act gcc gag ggc cgc atc gtt gaa gac gag gaa 1248

Met Phe Leu Val Asp Thr Ala Glu Gly Arg Ile Val Glu Asp Glu Glu

405 410 415

atc aag cag aaa tta agc gaa gcg cag cca tat ggt gag tgg att cgc 1296

Ile Lys Gln Lys Leu Ser Glu Ala Gln Pro Tyr Gly Glu Trp Ile Arg

420 425 430

gat aat ttt gtg cat ctg gat cgt ctg cct cag aca cgc tac aac tac 1344

Asp Asn Phe Val His Leu Asp Arg Leu Pro Gln Thr Arg Tyr Asn Tyr

435 440 445

atg gcg cac tct cgt gct gtg ttg cgt cag cgt gtt ttc gga atc act 1392

Met Ala His Ser Arg Ala Val Leu Arg Gln Arg Val Phe Gly Ile Thr

450 455 460

gaa gaa gat gtg gat ttg ttg ctg ctg ccg atg gcc cgc cag ggt gct 1440

Glu Glu Asp Val Asp Leu Leu Leu Leu Pro Met Ala Arg Gln Gly Ala

465 470 475 480

gag gcg att ggt tcc atg ggt tcg gat acg cca att gcg gcg cta tcc 1488

Glu Ala Ile Gly Ser Met Gly Ser Asp Thr Pro Ile Ala Ala Leu Ser

485 490 495

cag cga cca cgc atg ctt tat gat ttc ttc gcg cag cgc ttt gct cag 1536

Gln Arg Pro Arg Met Leu Tyr Asp Phe Phe Ala Gln Arg Phe Ala Gln

500 505 510

gtg aca aac cca ccg ttg gac tct atc cgc gaa aag cct gtg acc agc 1584

Val Thr Asn Pro Pro Leu Asp Ser Ile Arg Glu Lys Pro Val Thr Ser

515 520 525

atg ttc act ttg ttg ggt gcg cag tct gac gtg ctc aat ccg ggt cct 1632

Met Phe Thr Leu Leu Gly Ala Gln Ser Asp Val Leu Asn Pro Gly Pro

530 535 540

gat gcg gcg cga cgt atc cgt ttg gaa tcg ccg atc att gat aac cat 1680

Asp Ala Ala Arg Arg Ile Arg Leu Glu Ser Pro Ile Ile Asp Asn His

545 550 555 560

gag ctg gcc acc ttg atc aat gcc aac gcg cat ggt gag tgg gat tcc 1728

Glu Leu Ala Thr Leu Ile Asn Ala Asn Ala His Gly Glu Trp Asp Ser

565 570 575

ttt ggt gct gct gta att tct ggt ttg tac cca gtg gct cac cat ggt 1776

Phe Gly Ala Ala Val Ile Ser Gly Leu Tyr Pro Val Ala His His Gly

580 585 590

gcc ggc atg aag gct gcg att gct cgt gtg cgc cgc gag gtt tct gaa 1824

Ala Gly Met Lys Ala Ala Ile Ala Arg Val Arg Arg Glu Val Ser Glu

595 600 605

gca atc cgc aat ggc aag acg ttg atc gtg ctg tcg gat cgt gaa tct 1872

Ala Ile Arg Asn Gly Lys Thr Leu Ile Val Leu Ser Asp Arg Glu Ser

610 615 620

gat gag cgc atg gca cct atc cct gcg ctg ctg ctg act tcc gct gtg 1920

Asp Glu Arg Met Ala Pro Ile Pro Ala Leu Leu Leu Thr Ser Ala Val

625 630 635 640

cat cag tac ttg gtg cag caa cgt acc cgt acc cag tgc tcc ctg gtg 1968

His Gln Tyr Leu Val Gln Gln Arg Thr Arg Thr Gln Cys Ser Leu Val

645 650 655

gtg gaa tcc ggc gat gcc cgc gag gtt cat cac ctg gcg atg ctc att 2016

Val Glu Ser Gly Asp Ala Arg Glu Val His His Leu Ala Met Leu Ile

660 665 670

ggt ttt ggt gcc gat gcg atc aac ccg tac atg gca ttt gaa acc atc 2064

Gly Phe Gly Ala Asp Ala Ile Asn Pro Tyr Met Ala Phe Glu Thr Ile

675 680 685

gat gag ctg cgc atg aag ggt cag ttg ggt gat ctt tct ttg gat gag 2112

Asp Glu Leu Arg Met Lys Gly Gln Leu Gly Asp Leu Ser Leu Asp Glu

690 695 700

gca tcc cga aac tac atc aag gca gcc acc act ggt gtg ctg aag gtg 2160

Ala Ser Arg Asn Tyr Ile Lys Ala Ala Thr Thr Gly Val Leu Lys Val

705 710 715 720

atg tcc aag atg ggc att gca acg gtg tct tcg tac cgt ggc gcg cag 2208

Met Ser Lys Met Gly Ile Ala Thr Val Ser Ser Tyr Arg Gly Ala Gln

725 730 735

ctt gcc gat gtc act ggt ctg cac cag gat ctc ctg gac aac tac ttc 2256

Leu Ala Asp Val Thr Gly Leu His Gln Asp Leu Leu Asp Asn Tyr Phe

740 745 750

ggt ggt att gct tca cca att tct ggc atc ggt ctg gat gaa gtt gca 2304

Gly Gly Ile Ala Ser Pro Ile Ser Gly Ile Gly Leu Asp Glu Val Ala

755 760 765

gct gac gta gaa gct cgt cac cgc agc gca ttt ttg cca cgc cct gaa 2352

Ala Asp Val Glu Ala Arg His Arg Ser Ala Phe Leu Pro Arg Pro Glu

770 775 780

gag cac gct cac cgt gaa ttg gat ttg ggt ggt gaa tac aag tgg cgc 2400

Glu His Ala His Arg Glu Leu Asp Leu Gly Gly Glu Tyr Lys Trp Arg

785 790 795 800

cgc gaa ggt gaa tac cac ctg ttc aac cca gaa acc atc ttc aag ctg 2448

Arg Glu Gly Glu Tyr His Leu Phe Asn Pro Glu Thr Ile Phe Lys Leu

805 810 815

cag cat gca acg cgt tct ggc agc tac gag att ttc aag gat tac acc 2496

Gln His Ala Thr Arg Ser Gly Ser Tyr Glu Ile Phe Lys Asp Tyr Thr

820 825 830

cgc aag gtt gat gat caa tcc act cgc ttg ggt act att cgt gga ctg 2544

Arg Lys Val Asp Asp Gln Ser Thr Arg Leu Gly Thr Ile Arg Gly Leu

835 840 845

ttt gag ttc agc acg gac cgc aag cca att tcg gtg tct gag gtg gag 2592

Phe Glu Phe Ser Thr Asp Arg Lys Pro Ile Ser Val Ser Glu Val Glu

850 855 860

ccg gtc agt gag atc gtg aag cgt ttc tcc act ggt gcg atg tct tat 2640

Pro Val Ser Glu Ile Val Lys Arg Phe Ser Thr Gly Ala Met Ser Tyr

865 870 875 880

ggc tcg att tct gct gaa gcc cat gag gtc ttg gcc atc gcc atg aac 2688

Gly Ser Ile Ser Ala Glu Ala His Glu Val Leu Ala Ile Ala Met Asn

885 890 895

cga ctg ggc ggt atg tcc aac tcc ggc gaa ggt ggc gag gac gcc cgc 2736

Arg Leu Gly Gly Met Ser Asn Ser Gly Glu Gly Gly Glu Asp Ala Arg

900 905 910

cga ttt gat gtg gaa ccc aac ggt gac tgg aag cgc tct gcc att aag 2784

Arg Phe Asp Val Glu Pro Asn Gly Asp Trp Lys Arg Ser Ala Ile Lys

915 920 925

cag gtg gcc tcg gga cgt ttc ggc gtg acc agc cac tac ttg aac aac 2832

Gln Val Ala Ser Gly Arg Phe Gly Val Thr Ser His Tyr Leu Asn Asn

930 935 940

tgc acc gat att cag atc aag atg gca cag ggc gca aag ccc ggt gaa 2880

Cys Thr Asp Ile Gln Ile Lys Met Ala Gln Gly Ala Lys Pro Gly Glu

945 950 955 960

ggt ggc cag ctg cca cca aac aag gtg tac cca tgg gtt gca gaa gtc 2928

Gly Gly Gln Leu Pro Pro Asn Lys Val Tyr Pro Trp Val Ala Glu Val

965 970 975

cgc atc acc acc cca ggc gtt ggt ctg att tcc cct cca cca cac cac 2976

Arg Ile Thr Thr Pro Gly Val Gly Leu Ile Ser Pro Pro Pro His His

980 985 990

gat att tac tcc att gag gat ctg gct cag ctg atc cac gac ctg aag 3024

Asp Ile Tyr Ser Ile Glu Asp Leu Ala Gln Leu Ile His Asp Leu Lys

995 1000 1005

aac gct aac cca cgc gca cga atc cac gtg aag cta gtg gca gaa caa 3072

Asn Ala Asn Pro Arg Ala Arg Ile His Val Lys Leu Val Ala Glu Gln

1010 1015 1020

ggc gtg ggc acc gtt gcc gca ggt gtg tcc aaa gca cac gct gat gtg 3120

Gly Val Gly Thr Val Ala Ala Gly Val Ser Lys Ala His Ala Asp Val

1025 1030 1035 1040

gtg ctt att tcc ggc cac gat ggc gga act ggc gca tct cct ttg acc 3168

Val Leu Ile Ser Gly His Asp Gly Gly Thr Gly Ala Ser Pro Leu Thr

1045 1050 1055

tcc ctg aag cat gcc ggt ggt cca tgg gag ttg ggc ttg gct gaa acc 3216

Ser Leu Lys His Ala Gly Gly Pro Trp Glu Leu Gly Leu Ala Glu Thr

1060 1065 1070

cag caa acg ttg ctg ctc aac ggc ctg cgc gat cgt att cgc gtg cag 3264

Gln Gln Thr Leu Leu Leu Asn Gly Leu Arg Asp Arg Ile Arg Val Gln

1075 1080 1085

tgc gat ggt cag ctg aaa act ggc cga gac gtg gtt atc gca gct ctt 3312

Cys Asp Gly Gln Leu Lys Thr Gly Arg Asp Val Val Ile Ala Ala Leu

1090 1095 1100

ctc ggt gcc gaa gaa ttc ggt ttt gcc acc gca ccg ctg gtg gtt gaa 3360

Leu Gly Ala Glu Glu Phe Gly Phe Ala Thr Ala Pro Leu Val Val Glu

1105 1110 1115 1120

ggc tgc atc atg atg cgc gtc tgc cac ctg gac acc tgc ccg gtg ggt 3408

Gly Cys Ile Met Met Arg Val Cys His Leu Asp Thr Cys Pro Val Gly

1125 1130 1135

atc gct acc cag aac ccg gat ttg cgt tcc aag ttc acc ggc aag gct 3456

Ile Ala Thr Gln Asn Pro Asp Leu Arg Ser Lys Phe Thr Gly Lys Ala

1140 1145 1150

gaa cac gtg gtc aac ttc ttc acc ttc atc gcc cag gaa gtc cgt gag 3504

Glu His Val Val Asn Phe Phe Thr Phe Ile Ala Gln Glu Val Arg Glu

1155 1160 1165

tac ttg gca cag ctt ggt ttc cgc tct att gat gaa gcc gtc gga caa 3552

Tyr Leu Ala Gln Leu Gly Phe Arg Ser Ile Asp Glu Ala Val Gly Gln

1170 1175 1180

gcc cag gtg ctg cgc aag cgt tcc gga atc cca gct gat tcc cgc gca 3600

Ala Gln Val Leu Arg Lys Arg Ser Gly Ile Pro Ala Asp Ser Arg Ala

1185 1190 1195 1200

gca cac ctg gat ttg agc cca att ttc cat cgc cca gaa act cca cac 3648

Ala His Leu Asp Leu Ser Pro Ile Phe His Arg Pro Glu Thr Pro His

1205 1210 1215

ttc cca act cag gat gtg cgt tgc acc aag acc cag gaa cac agc cta 3696

Phe Pro Thr Gln Asp Val Arg Cys Thr Lys Thr Gln Glu His Ser Leu

1220 1225 1230

gaa aaa gcc ctg gac aac gca ttt att gat aag gct tcg gac acg atc 3744

Glu Lys Ala Leu Asp Asn Ala Phe Ile Asp Lys Ala Ser Asp Thr Ile

1235 1240 1245

acc cgt gcc gca gcg ggt gtg gaa acc agc att gtt att gat agc tcc 3792

Thr Arg Ala Ala Ala Gly Val Glu Thr Ser Ile Val Ile Asp Ser Ser

1250 1255 1260

atc agc aac gtc aac cgt tca gtt ggc acg atg ctg ggt tct gca gtc 3840

Ile Ser Asn Val Asn Arg Ser Val Gly Thr Met Leu Gly Ser Ala Val

1265 1270 1275 1280

agc cgc gtg gct ggt gcc caa ggt ttg cca gac ggc acc atc acc ttg 3888

Ser Arg Val Ala Gly Ala Gln Gly Leu Pro Asp Gly Thr Ile Thr Leu

1285 1290 1295

aat ctt caa ggc tgc gcc ggt aac tcc ttt ggc gcg ttc atc cca cga 3936

Asn Leu Gln Gly Cys Ala Gly Asn Ser Phe Gly Ala Phe Ile Pro Arg

1300 1305 1310

ggc atc acc atc aac ctc acc ggc gat gcc aat gac ttt gtg ggc aag 3984

Gly Ile Thr Ile Asn Leu Thr Gly Asp Ala Asn Asp Phe Val Gly Lys

1315 1320 1325

gga tta tct ggc gga aag att gtg atc aag cct tcc gct cag gct ccg 4032

Gly Leu Ser Gly Gly Lys Ile Val Ile Lys Pro Ser Ala Gln Ala Pro

1330 1335 1340

aag cag ctg aag aac aat cca aat atc att gcc gga aac gtg ctt gga 4080

Lys Gln Leu Lys Asn Asn Pro Asn Ile Ile Ala Gly Asn Val Leu Gly

1345 1350 1355 1360

tac ggc gca acc agt ggt gaa ttg ttc att cgt ggc cag gtc ggc gaa 4128

Tyr Gly Ala Thr Ser Gly Glu Leu Phe Ile Arg Gly Gln Val Gly Glu

1365 1370 1375

cgt ttc tgc gtc cgt aac tct ggc gcc acc gca gtg gtt gaa ggt atc 4176

Arg Phe Cys Val Arg Asn Ser Gly Ala Thr Ala Val Val Glu Gly Ile

1380 1385 1390

gga aac cac ggt tgt gag tac atg act ggc ggc cga gtc ctg gtt ttg 4224

Gly Asn His Gly Cys Glu Tyr Met Thr Gly Gly Arg Val Leu Val Leu

1395 1400 1405

ggc ccg gtt ggt gag aac ttt ggt gcc ggc atg tct ggt ggc att gca 4272

Gly Pro Val Gly Glu Asn Phe Gly Ala Gly Met Ser Gly Gly Ile Ala

1410 1415 1420

tac ctg gct aat tcc ccg gac cta aac cag aag atc aat ggc gaa ttg 4320

Tyr Leu Ala Asn Ser Pro Asp Leu Asn Gln Lys Ile Asn Gly Glu Leu

1425 1430 1435 1440

gtg gat gtt gtt cca ctg agc gct gac gat ctg acg tgg gct gat gag 4368

Val Asp Val Val Pro Leu Ser Ala Asp Asp Leu Thr Trp Ala Asp Glu

1445 1450 1455

ctc att gct cgc cac cgc gaa ctc acc gga tcc gag acc aag ctg cgt 4416

Leu Ile Ala Arg His Arg Glu Leu Thr Gly Ser Glu Thr Lys Leu Arg

1460 1465 1470

gca caa gat ttg gtg aaa atc atg ccg cgc gat ttc caa aaa gta ctc 4464

Ala Gln Asp Leu Val Lys Ile Met Pro Arg Asp Phe Gln Lys Val Leu

1475 1480 1485

aac atc atc gaa acg gcc cac gct gag ggc caa gac cca gca atc aag 4512

Asn Ile Ile Glu Thr Ala His Ala Glu Gly Gln Asp Pro Ala Ile Lys

1490 1495 1500

atc atg gag gca gtg agc 4530

Ile Met Glu Ala Val Ser

1505 1510

<210>162

<211>1510

<212>PRT

<213>谷氨酸棒状杆菌

<400>162

Met Lys Pro Gln Gly Leu Tyr Asn Pro Ala His Glu His Asp Ala Cys

1 5 10 15

Gly Val Ala Phe Ile Ala Asp Ile His Gly Arg Pro Ser Arg Ser Ile

20 25 30

Val Asp Arg Ala Leu Glu Ala Leu Arg Asn Ile Asp His Arg Gly Ala

35 40 45

Ala Gly Ala Glu Lys Asn Thr Gly Asp Gly Ala Gly Ile Leu Met Gln

50 55 60

Ile Pro Asp Gly Phe Tyr Arg Glu Val Ser Gly Ile Glu Leu Pro Glu

65 70 75 80

Ala Gly Glu Tyr Ala Thr Gly lle Ala Phe Leu Pro Arg Gly Arg Met

85 90 95

Ala Met Met Asp Ala Gln Lys Glu Ile Glu Arg Ile Ala Lys Gln Glu

100 105 110

Gly Ala Asp Val Leu Gly Trp Arg Met Val Pro Phe Asp Ser Arg Asp

115 120 125

Leu Gly Ser Met Ala Glu Glu Ala Met Pro Ser Phe Ala Gln Ile Phe

130 135 140

Leu Thr Val Pro Gly Lys Ser Gly Glu Asp Leu Asp Arg Val Met Phe

145 150 155 160

Phe Ile Arg Lys Arg Cys Glu Arg Glu Leu Gly Thr Thr Asn Gly Arg

165 170 175

Asp Thr Val Tyr Phe Pro Ser Leu Ser Ser Arg Thr Ile Ile Tyr Lys

180 185 190

Gly Met Leu Thr Thr Leu Gln Leu Glu Gly Phe Phe Glu Asp Leu Gly

195 200 205

Asp Ala Arg Leu Glu Ser Ala Ile Ala Ile Val His Ser Arg Phe Ser

210 215 220

Thr Asn Thr Phe Pro Ser Trp Pro Leu Ala His Pro Tyr Arg Phe Val

225 230 235 240

Ala His Asn Gly Glu Ile Asn Thr Val Arg Gly Asn Glu Asn Trp Met

245 250 255

Arg Ala Arg Glu Ala Leu Ile Lys Asn Asp Lys Leu Gly Asn Leu Ser

260 265 270

Ser Val Leu Pro Ile Cys Thr Pro Glu Gly Ser Asp Thr Ala Arg Phe

275 280 285

Asp Glu Ala Leu Glu Leu Leu His Leu Gly Gly Tyr Ser Leu Pro His

290 295 300

Ala Val Ala Met Met Ile Pro Gln Ala Trp Glu His Asn Lys Thr Leu

305 310 315 320

Ser Pro Glu Leu Arg Asp Phe Tyr Glu Tyr His Ser Cys Leu Met Glu

325 330 335

Pro Trp Asp Gly Pro Ala Ala Leu Ala Phe Thr Asp Gly Arg Phe Val

340 345 350

Gly Ala Val Leu Asp Arg Asn Gly Leu Arg Pro Gly Arg Ile Thr Ile

355 360 365

Thr Asp Ser Gly Leu Val Val Met Ala Ser Glu Ser Gly Val Leu Asp

370 375 380

Leu Arg Glu Glu Ser Val Val Lys Arg Thr Arg Val Gln Pro Gly Arg

385 390 395 400

Met Phe Leu Val Asp Thr Ala Glu Gly Arg Ile Val Glu Asp Glu Glu

405 410 415

Ile Lys Gln Lys Leu Ser Glu Ala Gln Pro Tyr Gly Glu Trp Ile Arg

420 425 430

Asp Asn Phe Val His Leu Asp Arg Leu Pro Gln Thr Arg Tyr Asn Tyr

435 440 445

Met Ala His Ser Arg Ala Val Leu Arg Gln Arg Val Phe Gly Ile Thr

450 455 460

Glu Glu Asp Val Asp Leu Leu Leu Leu Pro Met Ala Arg Gln Gly Ala

465 470 475 480

Glu Ala Ile Gly Ser Met Gly Ser Asp Thr Pro Ile Ala Ala Leu Ser

485 490 495

Gln Arg Pro Arg Met Leu Tyr Asp Phe Phe Ala Gln Arg Phe Ala Gln

500 505 510

Val Thr Asn Pro Pro Leu Asp Ser Ile Arg Glu Lys Pro Val Thr Ser

515 520 525

Met Phe Thr Leu Leu Gly Ala Gln Ser Asp Val Leu Asn Pro Gly Pro

530 535 540

Asp Ala Ala Arg Arg Ile Arg Leu Glu Ser Pro Ile Ile Asp Asn His

545 550 555 560

Glu Leu Ala Thr Leu Ile Asn Ala Asn Ala His Gly Glu Trp Asp Ser

565 570 575

Phe Gly Ala Ala Val Ile Ser Gly Leu Tyr Pro Val Ala His His Gly

580 585 590

Ala Gly Met Lys Ala Ala Ile Ala Arg Val Arg Arg Glu Val Ser Glu

595 600 605

Ala Ile Arg Asn Gly Lys Thr Leu Ile Val Leu Ser Asp Arg Glu Ser

610 615 620

Asp Glu Arg Met Ala Pro Ile Pro Ala Leu Leu Leu Thr Ser Ala Val

625 630 635 640

His Gln Tyr Leu Val Gln Gln Arg Thr Arg Thr Gln Cys Ser Leu Val

645 650 655

Val Glu Ser Gly Asp Ala Arg Glu Val His His Leu Ala Met Leu Ile

660 665 670

Gly Phe Gly Ala Asp Ala Ile Asn Pro Tyr Met Ala Phe Glu Thr Ile

675 680 685

Asp Glu Leu Arg Met Lys Gly Gln Leu Gly Asp Leu Ser Leu Asp Glu

690 695 700

Ala Ser Arg Asn Tyr Ile Lys Ala Ala Thr Thr Gly Val Leu Lys Val

705 710 715 720

Met Ser Lys Met Gly Ile Ala Thr Val Ser Ser Tyr Arg Gly Ala Gln

725 730 735

Leu Ala Asp Val Thr Gly Leu His Gln Asp Leu Leu Asp Asn Tyr Phe

740 745 750

Gly Gly Ile Ala Ser Pro Ile Ser Gly Ile Gly Leu Asp Glu Val Ala

755 760 765

Ala Asp Val Glu Ala Arg His Arg Ser Ala Phe Leu Pro Arg Pro Glu

770 775 780

Glu His Ala His Arg Glu Leu Asp Leu Gly Gly Glu Tyr Lys Trp Arg

785 790 795 800

Arg Glu Gly Glu Tyr His Leu Phe Asn Pro Glu Thr Ile Phe Lys Leu

805 810 815

Gln His Ala Thr Arg Ser Gly Ser Tyr Glu Ile Phe Lys Asp Tyr Thr

820 825 830

Arg Lys Val Asp Asp Gln Ser Thr Arg Leu Gly Thr Ile Arg Gly Leu

835 840 845

Phe Glu Phe Ser Thr Asp Arg Lys Pro Ile Ser Val Ser Glu Val Glu

850 855 860

Pro Val Ser Glu Ile Val Lys Arg Phe Ser Thr Gly Ala Met Ser Tyr

865 870 875 880

Gly Ser Ile Ser Ala Glu Ala His Glu Val Leu Ala Ile Ala Met Asn

885 890 895

Arg Leu Gly Gly Met Ser Asn Ser Gly Glu Gly Gly Glu Asp Ala Arg

900 905 910

Arg Phe Asp Val Glu Pro Asn Gly Asp Trp Lys Arg Ser Ala Ile Lys

915 920 925

Gln Val Ala Ser Gly Arg Phe Gly Val Thr Ser His Tyr Leu Asn Asn

930 935 940

Cys Thr Asp Ile Gln Ile Lys Met Ala Gln Gly Ala Lys Pro Gly Glu

945 950 955 960

Gly Gly Gln Leu Pro Pro Asn Lys Val Tyr Pro Trp Val Ala Glu Val

965 970 975

Arg Ile Thr Thr Pro Gly Val Gly Leu Ile Ser Pro Pro Pro His His

980 985 990

Asp Ile Tyr Ser Ile Glu Asp Leu Ala Gln Leu Ile His Asp Leu Lys

995 1000 1005

Asn Ala Asn Pro Arg Ala Arg Ile His Val Lys Leu Val Ala Glu Gln

1010 1015 1020

Gly Val Gly Thr Val Ala Ala Gly Val Ser Lys Ala His Ala Asp Val

1025 1030 1035 1040

Val Leu Ile Ser Gly His Asp Gly Gly Thr Gly Ala Ser Pro Leu Thr

1045 1050 1055

Ser Leu Lys His Ala Gly Gly Pro Trp Glu Leu Gly Leu Ala Glu Thr

1060 1065 1070

Gln Gln Thr Leu Leu Leu Asn Gly Leu Arg Asp Arg Ile Arg Val Gln

1075 1080 1085

Cys Asp Gly Gln Leu Lys Thr Gly Arg Asp Val Val Ile Ala Ala Leu

1090 1095 1100

Leu Gly Ala Glu Glu Phe Gly Phe Ala Thr Ala Pro Leu Val Val Glu

1105 1110 1115 1120

Gly Cys Ile Met Met Arg Val Cys His Leu Asp Thr Cys Pro Val Gly

1125 1130 1135

Ile Ala Thr Gln Asn Pro Asp Leu Arg Ser Lys Phe Thr Gly Lys Ala

1140 1145 1150

Glu His Val Val Asn Phe Phe Thr Phe Ile Ala Gln Glu Val Arg Glu

1155 1160 1165

Tyr Leu Ala Gln Leu Gly Phe Arg Ser Ile Asp Glu Ala Val Gly Gln

1170 1175 1180

Ala Gln Val Leu Arg Lys Arg Ser Gly Ile Pro Ala Asp Ser Arg Ala

1185 1190 1195 1200

Ala His Leu Asp Leu Ser Pro Ile Phe His Arg Pro Glu Thr Pro His

1205 1210 1215

Phe Pro Thr Gln Asp Val Arg Cys Thr Lys Thr Gln Glu His Ser Leu

1220 1225 1230

Glu Lys Ala Leu Asp Asn Ala Phe Ile Asp Lys Ala Ser Asp Thr Ile

1235 1240 1245

Thr Arg Ala Ala Ala Gly Val Glu Thr Ser Ile Val Ile Asp Ser Ser

1250 1255 1260

Ile Ser Asn Val Asn Arg Ser Val Gly Thr Met Leu Gly Ser Ala Val

1265 1270 1275 1280

Ser Arg Val Ala Gly Ala Gln Gly Leu Pro Asp Gly Thr Ile Thr Leu

1285 1290 1295

Asn Leu Gln Gly Cys Ala Gly Asn Ser Phe Gly Ala Phe Ile Pro Arg

1300 1305 1310

Gly Ile Thr Ile Asn Leu Thr Gly Asp Ala Asn Asp Phe Val Gly Lys

1315 1320 1325

Gly Leu Ser Gly Gly Lys Ile Val Ile Lys Pro Ser Ala Gln Ala Pro

1330 1335 1340

Lys Gln Leu Lys Asn Asn Pro Asn Ile Ile Ala Gly Asn Val Leu Gly

1345 1350 1355 1360

Tyr Gly Ala Thr Ser Gly Glu Leu Phe Ile Arg Gly Gln Val Gly Glu

1365 1370 1375

Arg Phe Cys Val Arg Asn Ser Gly Ala Thr Ala Val Val Glu Gly Ile

1380 1385 1390

Gly Asn His Gly Cys Glu Tyr Met Thr Gly Gly Arg Val Leu Val Leu

1395 1400 1405

Gly Pro Val Gly Glu Asn Phe Gly Ala Gly Met Ser Gly Gly Ile Ala

1410 1415 1420

Tyr Leu Ala Asn Ser Pro Asp Leu Asn Gln Lys Ile Asn Gly Glu Leu

1425 1430 1435 1440

Val Asp Val Val Pro Leu Ser Ala Asp Asp Leu Thr Trp Ala Asp Glu

1445 1450 1455

Leu Ile Ala Arg His Arg Glu Leu Thr Gly Ser Glu Thr Lys Leu Arg

1460 1465 1470

Ala Gln Asp Leu Val Lys Ile Met Pro Arg Asp Phe Gln Lys Val Leu

1475 1480 1485

Asn Ile Ile Glu Thr Ala His Ala Glu Gly Gln Asp Pro Ala Ile Lys

1490 1495 1500

Ile Met Glu Ala Val Ser

1505 1510

<210>163

<211>1455

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(1)..(1455)

<223>RXA07001

<400>163

atg aag tac ttg gtg gcg ctg gga aga agc ctt cgt tat ggg agg tct 48

Met Lys Tyr Leu Val Ala Leu Gly Arg Ser Leu Arg Tyr Gly Arg Ser

1 5 10 15

ccc aga cac aat cga ata cgg gcc gga tat cca tct cgg ctc atc acc 96

Pro Arg His Asn Arg Ile Arg Ala Gly Tyr Pro Ser Arg Leu Ile Thr

20 25 30

ccg ctt ttt atc aag aaa gat gag gac ctc ttg aag cgt ctt act cgc 144

Pro Leu Phe Ile Lys Lys Asp Glu Asp Leu Leu Lys Arg Leu Thr Arg

35 40 45

atc gca tcc atc agc atg gcc tcc atg ctc gcc gcc gca agt ctc gtc 192

Ile Ala Ser Ile Ser Met Ala Ser Met Leu Ala Ala Ala Ser Leu Val

50 55 60

gcg tgc tcc ggc tcc acc gac gag gaa ggc gat gtt tac ttc ctg aac 240

Ala Cys Ser Gly Ser Thr Asp Glu Glu Gly Asp Val Tyr Phe Leu Asn

65 70 75 80

ttc aag cct gaa cag gac gtg gca tac cag gaa atc gca aag gcc tac 288

Phe Lys Pro Glu Gln Asp Val Ala Tyr Gln Glu Ile Ala Lys Ala Tyr

85 90 95

act gaa gag acc ggc gtt aag gtc aag gtc gtt act gcc gcc tcc ggc 336

Thr Glu Glu Thr Gly Val Lys Val Lys Val Val Thr Ala Ala Ser Gly

100 105 110

tcc tat gag cag acc ctc aag gcc gag att ggc aag gac gaa gcc ccg 384

Ser Tyr Glu Gln Thr Leu Lys Ala Glu Ile Gly Lys Asp Glu Ala Pro

115 120 125

act ctc ttc cag gtc aat ggc cca gcc ggc ttc atc act tgg cag gac 432

Thr Leu Phe Gln Val Asn Gly Pro Ala Gly Phe Ile Thr Trp Gln Asp

130 135 140

tac atg gca gat atg tcg gac acc gag gta gct aag cag ctg acc gac 480

Tyr Met Ala Asp Met Ser Asp Thr Glu Val Ala Lys Gln Leu Thr Asp

145 150 155 160

gac att ccg ccg ctg acc acc gag gat ggc gag gta cgt ggc gtt ccg 528

Asp Ile Pro Pro Leu Thr Thr Glu Asp Gly Glu Val Arg Gly Val Pro

165 170 175

ttc gcc gtc gag ggc ttc ggc atc atc tac aac gac gag atc ttc gac 576

Phe Ala Val Glu Gly Phe Gly Ile Ile Tyr Asn Asp Glu Ile Phe Asp

180 185 190

aag tac atc gcc acg tcc ggc gca aag atc aag tcc acg gat gag atc 624

Lys Tyr Ile Ala Thr Ser Gly Ala Lys Ile Lys Ser Thr Asp Glu Ile

195 200 205

acg agc tac cag aag ctc aag gaa gtc gcc gag gat atg cag gca aag 672

Thr Ser Tyr Gln Lys Leu Lys Glu Val Ala Glu Asp Met Gln Ala Lys

210 215 220

aag gac gag ctc ggt atc gaa ggc gcc ttc gcc tcc acc tcg ctg aca 720

Lys Asp Glu Leu Gly Ile Glu Gly Ala Phe Ala Ser Thr Ser Leu Thr

225 230 235 240

tcc ggt gag gac tgg cgt tgg cag acc cac ctg gcc aac gct ccg atc 768

Ser Gly Glu Asp Trp Arg Trp Gln Thr His Leu Ala Asn Ala Pro Ile

245 250 255

tgg cag gag tac cag gac aag gga gtc gag gac acc aac gag atc gag 816

Trp Gln Glu Tyr Gln Asp Lys Gly Val Glu Asp Thr Asn Glu Ile Glu

260 265 270

ttc tcc tac aac aag gag tac aag aac ctt ttc gat ctc tac ctt gag 864

Phe Ser Tyr Asn Lys Glu Tyr Lys Asn Leu Phe Asp Leu Tyr Leu Glu

275 280 285

aac tcc acc gta gaa aag tct ctt gcg ccg tct aag acg gtg tct gat 912

Asn Ser Thr Val Glu Lys Ser Leu Ala Pro Ser Lys Thr Val Ser Asp

290 295 300

tcc atg gct gag ttc gca cag ggc aag gcc gct atg gtt cag aac ggt 960

Ser Met Ala Glu Phe Ala Gln Gly Lys Ala Ala Met Val Gln Asn Gly

305 310 315 320

aac tgg gca tgg tcc cag att tcc gag act tct ggc aac gtg gtc aag 1008

Asn Trp Ala Trp Ser Gln Ile Ser Glu Thr Ser Gly Asn Val Val Lys

325 330 335

gaa gac aag atc aag ttc ctg ccc atg tac atg ggt ctg cca gat gaa 1056

Glu Asp Lys Ile Lys Phe Leu Pro Met Tyr Met Gly Leu Pro Asp Glu

340 345 350

gaa aag cac ggc atc aac gtc ggt acc gag aac tat ttg ggc gtg aac 1104

Glu Lys His Gly Ile Asn Val Gly Thr Glu Asn Tyr Leu Gly Val Asn

355 360 365

tct gag gcc tcc gag gtc gac cag cag gcc acc aag gac ttc gtg gat 1152

Ser Glu Ala Ser Glu Val Asp Gln Gln Ala Thr Lys Asp Phe Val Asp

370 375 380

tgg ctg ttt acc tct gaa gct ggc aag gag cac gtg gtg aag gac ctt 1200

Trp Leu Phe Thr Ser Glu Ala Gly Lys Glu His Val Val Lys Asp Leu

385 390 395 400

ggc ttc atc gca ccg ttc gaa agc tac acc gct gag aac acc ccg aat 1248

Gly Phe Ile Ala Pro Phe Glu Ser Tyr Thr Ala Glu Asn Thr Pro Asn

405 410 415

gac ccc ctt tct gag caa gtc gcg gaa gct atc gct aac aag gat ctg 1296

Asp Pro Leu Ser Glu Gln Val Ala Glu Ala Ile Ala Asn Lys Asp Leu

420 425 430

acc acc tac ccg tgg aac ttc cag tac ttc ccg tcc cag cag ttc aag 1344

Thr Thr Tyr Pro Trp Asn Phe Gln Tyr Phe Pro Ser Gln Gln Phe Lys

435 440 445

gat gac ttc ggc cag gat ctg tcg cag tac gcc tcc gga aag ctg aag 1392

Asp Asp Phe Gly Gln Asp Leu Ser Gln Tyr Ala Ser Gly Lys Leu Lys

450 455 460

tgg gag gac gtc gtc aag cac ttc aat gac aac tgg gct gct gag aag 1440

Trp Glu Asp Val Val Lys His Phe Asn Asp Asn Trp Ala Ala Glu Lys

465 470 475 480

gaa tcc aac tgg ggc 1455

Glu Ser Asn Trp Gly

485

<210>164

<211>485

<212>PRT

<213>谷氨酸棒状杆菌

<400>164

Met Lys Tyr Leu Val Ala Leu Gly Arg Ser Leu Arg Tyr Gly Arg Ser

1 5 10 15

Pro Arg His Asn Arg Ile Arg Ala Gly Tyr Pro Ser Arg Leu Ile Thr

20 25 30

Pro Leu Phe Ile Lys Lys Asp Glu Asp Leu Leu Lys Arg Leu Thr Arg

35 40 45

Ile Ala Ser Ile Ser Met Ala Ser Met Leu Ala Ala Ala Ser Leu Val

50 55 60

Ala Cys Ser Gly Ser Thr Asp Glu Glu Gly Asp Val Tyr Phe Leu Asn

65 70 75 80

Phc Lys Pro Glu Gln Asp Val Ala Tyr Gln Glu Ile Ala Lys Ala Tyr

85 90 95

Thr Glu Glu Thr Gly Val Lys Val Lys Val Val Thr Ala Ala Ser Gly

100 105 110

Ser Tyr Glu Gln Thr Leu Lys Ala Glu Ile Gly Lys Asp Glu Ala Pro

115 120 125

Thr Leu Phe Gln Val Asn Gly Pro Ala Gly Phe Ile Thr Trp Gln Asp

130 135 140

Tyr Met Ala Asp Met Ser Asp Thr Glu Val Ala Lys Gln Leu Thr Asp

145 150 155 160

Asp Ile Pro Pro Leu Thr Thr Glu Asp Gly Glu Val Arg Gly Val Pro

165 170 175

Phe Ala Val Glu Gly Phe Gly Ile Ile Tyr Asn Asp Glu Ile Phe Asp

180 185 190

Lys Tyr Ile Ala Thr Ser Gly Ala Lys Ile Lys Ser Thr Asp Glu Ile

195 200 205

Thr Ser Tyr Gln Lys Leu Lys Glu Val Ala Glu Asp Met Gln Ala Lys

210 215 220

Lys Asp Glu Leu Gly Ile Glu Gly Ala Phe Ala Ser Thr Ser Leu Thr

225 230 235 240

Ser Gly Glu Asp Trp Arg Trp Gln Thr His Leu Ala Asn Ala Pro Ile

245 250 255

Trp Gln Glu Tyr Gln Asp Lys Gly Val Glu Asp Thr Asn Glu Ile Glu

260 265 270

Phe Ser Tyr Asn Lys Glu Tyr Lys Asn Leu Phe Asp Leu Tyr Leu Glu

275 280 285

Asn Ser Thr Val Glu Lys Ser Leu Ala Pro Ser Lys Thr Val Ser Asp

290 295 300

Ser Met Ala Glu Phe Ala Gln Gly Lys Ala Ala Met Val Gln Asn Gly

305 310 315 320

Asn Trp Ala Trp Ser Gln Ile Ser Glu Thr Ser Gly Asn Val Val Lys

325 330 335

Glu Asp Lys Ile Lys Phe Leu Pro Met Tyr Met Gly Leu Pro Asp Glu

340 345 350

Glu Lys His Gly Ile Asn Val Gly Thr Glu Asn Tyr Leu Gly Val Asn

355 360 365

Ser Glu Ala Ser Glu Val Asp Gln Gln Ala Thr Lys Asp Phe Val Asp

370 375 380

Trp Leu Phe Thr Ser Glu Ala Gly Lys Glu His Val Val Lys Asp Leu

385 390 395 400

Gly Phe Ile Ala Pro Phe Glu Ser Tyr Thr Ala Glu Asn Thr Pro Asn

405 410 415

Asp Pro Leu Ser Glu Gln Val Ala Glu Ala Ile Ala Asn Lys Asp Leu

420 425 430

Thr Thr Tyr Pro Trp Asn Phe Gln Tyr Phe Pro Ser Gln Gln Phe Lys

435 440 445

Asp Asp Phe Gly Gln Asp Leu Ser Gln Tyr Ala Ser Gly Lys Leu Lys

450 455 460

Trp Glu Asp Val Val Lys His Phe Asn Asp Asn Trp Ala Ala Glu Lys

465 470 475 480

Glu Ser Asn Trp Gly

485

Claims

1.分离的核酸分子，其编码的多肽所具有的氨基酸序列在每种情况下均涉及表1/第2列，其中在表1/第4列所示氨基酸位置处的核酸分子所编码的蛋白原氨基酸与表1/第5列相同行所示的特定氨基酸不同。

2.如权利要求1所述的分离的核酸分子，其中在表1/第4列中所示氨基酸位置处的核酸分子编码表1/第6列相同行所示的氨基酸。

3.载体，其包含至少一个如权利要求1所述的核酸序列。

4.宿主细胞，其用至少一种如权利要求3所述的载体转染。

5.如权利要求4所述的宿主细胞，其中所述核酸分子的表达调节所述细胞精细化学品的生产率。

6.制备精细化学品的方法，其包括培养已用至少一种如权利要求3所述的载体转染的细胞，从而产生精细化学品。

7.如权利要求6所述的方法，其中精细化学品是氨基酸。

8.如权利要求7所述的方法，其中所述氨基酸是赖氨酸。