CN1582300A - 编码新蛋白的基因 - Google Patents
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Abstract
本发明涉及新的核酸分子、其用于构建遗传改良微生物的用途以及借助于所述遗传改良微生物制备精细化学品,尤其是氨基酸的方法。
Description
发明背景
细胞中天然进行的代谢过程所产生的特定产物和副产物可以用于工业的许多分支中,包括食品工业、动物饲料工业、化妆品工业及制药工业。这些分子合起来称为“精细化学品”,其包括有机酸、蛋白原性(protainogenic)和非蛋白原性氨基酸、核苷酸和核苷、脂类和脂肪酸、二醇、碳水化合物、芳香化合物、维生素和辅因子、以及酶。它们的最好的产生途径是大规模培养细菌,针对不同的特定情况,这些细菌被开发出来以产生并分泌大量的期望分子。一种对此目的尤其适用的生物体是谷氨酸棒状杆菌(Corynebacterium glutamicum),其为革兰氏阳性的非致病细菌。通过菌株选择,已开发了许多突变菌株,它们能产生各种期望化合物。但是,对具有提高的特定分子产量的菌株的筛选是一种费时且困难的方法。
发明简述
本发明提供了新的可用于谷氨酸棒状杆菌或相关菌种的鉴定或分类的核酸分子。谷氨酸棒状杆菌为革兰氏阳性需氧细菌,其广泛地用在工业上用于大规模产生多种精细化学品,也用于降解碳氢化合物(如在原油泄漏物中),以及用于氧化萜类化合物。本发明的核酸分子因此可用于鉴定能如通过发酵法用来产生精细化学品的微生物。虽然谷氨酸棒状杆菌自身为非致病的,但它与作为人类的主要病原体的其它谷氨酸棒状杆菌菌种,如白喉棒状杆菌(Corynebacterium diphtheriae)(白喉的致病因子)有亲缘关系。所以能够鉴定棒状杆菌的存在也具有显著的临床重要性,例如诊断应用。而且,所述核酸分子可作为谷氨酸棒状杆菌或相关生物基因组作图的参考点。
这些新的核酸分子编码文中称作标记和精细化学品生产(MCP)蛋白的蛋白。这些MCP蛋白可能,例如直接或间接地在谷氨酸棒状杆菌中参与精细化学品的生产。本发明的MCP蛋白还可能参与碳氢化合物的降解或萜类化合物的氧化。所述蛋白可用于鉴定谷氨酸棒状杆菌或与谷氨酸棒状杆菌相关的生物体;蛋白混合物中存在对谷氨酸棒状杆菌及相关种特异的MCP蛋白可以说明样品中存在所述细菌之一。此外,这些MCP-蛋白在植物或动物中可能具有与病害状态或疾病有关的同系物;因此,这些蛋白可作为药物筛选和开发治疗化性合物的有用的药学靶标。
由于可得到用于谷氨酸棒状杆菌中的克隆载体,例如在Sinskey等,美国专利No.4,649,119中所公开的那些,和用于遗传操作谷氨酸棒状杆菌和相关短杆菌种(如乳糖发酵短杆菌(lactofermentum))的技术(Yoshihama等,细菌学杂志(J.Bacteriol.)162:591-597(1985);Katsumata等,细菌学杂志(J.Bacteriol.)159:306-311(1984);和Santamaria等,普通微生物学杂志(J.Gen.Microbiol.)130:2237-2246(1984)),本发明的核酸分子可用于遗传操作所述的生物体,以调节一种或多种精细化学品的产生。这种调节可由于操作本发明的基因的直接效果,或可由于此类操作的间接效果而发生。例如,有可能通过修饰参与精细化学品生物合成或者降解的蛋白的活性(即,通过相应基因的突变),直接调节细胞合成或降解所述化合物的的能力,由此调节精细化学品的产率和/或生产效率。同样地,有可能通过调节调控精细化学品代谢途径的蛋白的活性直接加以影响,不论期望化合物的产生是上调还是下调,两种方式都将调节来自细胞的精细化学品的产率或生产效率。
精细化学品产生的间接调节也可以通过修饰本发明的蛋白的活性(即通过相应基因的突变),使细胞生长或分裂或维持存活或生产力的能力总体上得以提高而进行。从谷氨酸棒状杆菌生产精细化学品通常通过在经常是对生长或细胞分裂次最佳的条件下大规模发酵培养所述微生物来实现。修饰本发明的蛋白(如应激反应蛋白、细胞壁蛋白或者与细胞分裂或细胞生长发生所必需的化合物如核苷酸和氨基酸的代谢有关的蛋白)以至于所述条件下细胞的生存、生长和繁殖得以提高,将能够使所述修饰的谷氨酸棒状杆菌细胞在大规模培养中的数量和生产力提高,并且这接下来将提高一种或多种期望精细化学品的产率和/或生产效率。此外,细胞代谢途径必然地彼此相依并被共同调控。改变谷氨酸棒状杆菌任一代谢途径的活性(即改变参与此类途径的本发明蛋白之一的活性),可同时改变或者调节这个微生物中另一代谢途径的活性,而此另一代谢途径可能直接地涉及精细化学品的合成或降解。
本发明提供了编码这里称作为MCP蛋白的蛋白的新核酸分子,所述MCP蛋白能够,例如调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率,或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。编码MCP蛋白的核酸分子这里称作为MCP核酸分子。在优选的实施方案中,MCP蛋白能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或产率,或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。此类蛋白的实例是表1中所列基因编码的那些蛋白。
因此,本发明的一个方面涉及含有编码MCP蛋白或其生物学活性部分的核苷酸序列的分离的核酸分子(例如cDNA)和适合作为检测或扩增MCP编码核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在尤其优选的实施方案中,该分离的核酸分子含有集合A所列出的任一核酸序列或这些核酸序列之任一的编码区或其互补序列。在其他优选的实施方案中,该分离的核酸分子编码集合B中列出的氨基酸序列之一。本发明优选的MCP蛋白还优选具有至少一种本文所描述的MCP活性。
以下将序列表中的核酸序列以及下表1中描述的在相关位置处的序列修饰一起定义为集合A。
以下将序列表中的多肽序列以及下表1中描述的在相关位置处的序列修饰一起定义为集合B。
在另一个实施方案中,分离的核酸分子长度至少15个核苷酸并且在严紧条件下与含有集合A的核苷酸序列的核酸分子杂交。分离的核酸分子优选与天然发生的核酸分子对应。分离的核酸更优选编码天然发生的谷氨酸棒状杆菌MCP蛋白或其具生物活性的部分。
本发明的另一个方面涉及载体(例如重组表达载体),其含有本发明的核酸分子,还涉及已导入所述载体的宿主细胞。在一个实施方案中,通过使用在合适的培养基中培养的宿主细胞制备MCP蛋白。可以从培养基或宿主细胞中分离该MCP蛋白。
本发明的另一个方面涉及其中导入了MCP基因或其MCP基因被修饰的遗传修饰的微生物。在一个实施方案中,通过导入至少一种编码突变MCP序列的本发明核酸分子作为转基因,修饰了所述微生物的基因组。在另一个实施方案中,通过与修饰的MCP基因的同源重组,修饰(例如破坏功能)了所述微生物基因组中的内源性MCP基因。在一个优选的实施方案中,微生物属于棒状杆菌或短杆菌(Brevibacterium)属,尤其优选谷氨酸棒状杆菌。在一个优选的实施方案中,该微生物也被用于制备目标化合物,如氨基酸、尤其优选赖氨酸。
另一个优选的实施方案是具有一种以上的集合A中描述的核酸分子的宿主细胞。可用各种技术人员已知的方法制备这些宿主细胞。例如,可通过携带几种本发明核酸分子的载体转染这些细胞。然而,也可以使用载体每一种情况下向宿主细胞中导入一种本发明的核酸分子并从而同时或相继使用多个载体。从而可能构建携带许多乃至高达数百的本发明核酸序列的宿主细胞。这种积累常能够对宿主细胞的精细化学品的生产力产生超加性效果。
本发明的另一个方面涉及分离的MCP蛋白或其部分,例如具有生物学活性的部分。在优选的实施方案中,该分离的MCP蛋白或其部分能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率,或者能够作为谷氨酸棒状杆菌或相关生物体的鉴定标记。在另一个优选的实施方案中,该分离的MCP蛋白或其部分与集合B的氨基酸序列充分同源,以至该蛋白或其部分仍然能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率,或者能够作为谷氨酸棒状杆菌或相关生物体的鉴定标记。
此外,本发明涉及分离的MCP蛋白制品。在优选的实施方案中,该MCP蛋白含有集合B的氨基酸序列。在另一个优选的实施方案中,本发明涉及分离的完整长度的蛋白,其和集合B的完整氨基酸序列(其由集合A中的可读框编码)基本同源。
可以将MCP多肽或其生物学活性部分与非MCP多肽功能性地连接以产生融合蛋白。在优选的实施方案中,该融合蛋白和单独的MCP蛋白具有不同的活性,在其它优选的实施方案中,所述融合蛋白能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率,或者能够作为谷氨酸棒状杆菌或相关生物体的鉴定标记。在尤其优选的实施方案中,所述融合蛋白整合在宿主细胞中调节细胞对目的化合物的生产。
本发明的另一个方面涉及制备精细化学品的制备方法。该方法涉及含有可导致本发明MCP核酸分子表达的载体的细胞的培养,从而生产精细化学品。在优选的实施方案中,该方法还包括得到含有此载体的细胞的步骤,其中所述细胞被导致MCP核酸表达的载体转染。在另一个优选的实施方案中,所述方法还包括从培养物中得到精细化学品的步骤。在一个尤其优选的实施方案中,该细胞属于棒状杆菌属或短杆菌属。
本发明的另一个方面涉及调节微生物生产分子的方法。这些方法包括将细胞和调节MCP蛋白活性或MCP核酸表达的物质接触使得细胞的相关活性和没有所述物质存在时的相同活性相比被改变。在一个优选的实施方案中,针对一个或多个谷氨酸棒状杆菌MCP蛋白活性对细胞进行调节,以提高该微生物生产目标精细化学品的产率、产量和/或生产效率。调节MCP蛋白活性的物质可以是刺激MCP蛋白活性或MCP核酸表达的物质。刺激MCP蛋白活性或MCP核酸表达的物质的例子包括小分子、活性MCP蛋白和已经被导入细胞中的编码MCP蛋白的核酸。抑制MCP活性或MCP表达的物质的例子包括小分子和反义MCP核酸分子。
本发明的另一个方面涉及调节细胞中目的化合物的产率、产量和/或生产效率的方法,包括向细胞中导入MCP野生型基因或MCP突变基因,该基因或者保留在分开的质粒上或者整合到宿主细胞的基因组中。向基因组的整合可随机发生或者通过同源重组发生,从而使得天然基因被整合的拷贝所代替,导致细胞中目标化合物的生产被调节。在另一个优选的实施方案中,所述产率增加了。在另一个优选的实施方案中,化学品是精细化学品,其在一个特别优选的实施方案中是氨基酸。在一个特别优选的实施方案中,该氨基酸是L-赖氨酸。
发明详述
本发明提供了MCP核酸和MCP蛋白分子,它们可用于鉴定谷氨酸棒状杆菌或相关生物体,用于绘制谷氨酸棒状杆菌基因组(或密切相关生物体的基因组)图谱或者用于鉴定可用来例如通过发酵工艺产生精细化学品的微生物。由所述核酸编码的蛋白可用来直接或间接地调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率,可作为谷氨酸棒状杆菌或相关生物体的鉴定标记,可用于氧化萜类化合物,或者用来降解碳氢化合物,或者作为研发治疗性药学化合物的靶标。本发明的诸方面进一步阐述如下。
I.精细化学品
术语“精细化学品”为本领域所认知,其包括已用于多种工业的由生物体产生的分子,其中所述工业例如但不限于制药工业、农业工业和化妆品工业。此类化合物包括有机酸如酒石酸、衣康酸和二氨基庚二酸、蛋白原的和非蛋白原的氨基酸、嘌呤和嘧啶碱基、核苷和核苷酸(如在Rehm等人编辑的生物技术(Biotechnology)第6卷中Kuninaka,A.(1996)核苷酸和相关化合物(Nucleotides and related compounds),561-612页,VCH:Weinheim及其中所含参考文献中所述)、脂类、饱和和不饱和脂肪酸(如花生四烯酸)、二醇(如丙二醇和丁二醇)、碳水化合物(如透明质酸和海藻糖)、芳香化合物(如芳香胺、香草醛和靛蓝)、维生素和辅因子(参见如Uilmann工业化学百科全书(Ullmann’s Encyclopedia ofIndustrial Chemistry),A27卷,“维生素”(“Vitamins”),443-613页(1996)VCH:Weinheim及其中的参考文献;以及Ong,A.S.,Niki,E.和Packer,L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition,Lipids,Health,and Disease”Proceedings ofthe UNESCO/Confederation of Scientific and Technological Associationsin Malaysia,and the Society for Free Radical Research-Asia),1994年9月1-3日在马来西亚槟榔屿举行,AOCS Press,(1995))、酶以及在Gutcho(1983)通过发酵的化学品(Chemicals by Fermentation),Noyes DataCorporation,ISBN:0818805086和其中的参考文献中所述的所有其它化学品。这些精细化学品中的某些化学品的代谢和用途进一步阐述如下。
A.氨基酸代谢和用途
氨基酸包含所有蛋白质的基本结构单元,由此为所有生物体中正常细胞功能所必需。术语“氨基酸”为本领域所认知。蛋白原性氨基酸有20种,为蛋白质的结构单元,在蛋白质中它们通过肽键连接,非蛋白原性氨基酸(已知成百种非蛋白原的氨基酸)在蛋白质中通常不存在(参见Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)),A2卷,57-97页,VCH:Weinheim(1985))。氨基酸可为光学D-或L-构型,尽管在天然存在的蛋白质中发现的唯一类型通常为L-氢基酸。已充分说明了20种蛋白原性氨基酸中每一种在原核和真核细胞中的生物合成和降解途径(参见例如,Stryer,L.生物化学(Biochemistry),第三版,578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸),这样称呼是因为由于它们生物合成的复杂性,通常必须从食物中摄取它们,它们通过简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物能够合成这些氨基酸中的一部分氨基酸,但必需氨基酸必须从食物中摄取,以进行正常的蛋白质合成。
除了它们在蛋白质生物合成中的功能外,这些氨基酸本身还是令人感兴趣的化学品,并且许多已发现在人类食品、动物饲料、化学品、化妆品、农业和药学工业中具有多种应用。赖氨酸不仅对人的营养而且对单胃家畜如禽和猪都是一种重要的氨基酸。谷氨酸盐是最常用的调味添加剂(谷氨酸单钠盐,MSG),并且还用于食品工业,天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸均用于药学工业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸用于药学工业和化妆品工业。苏氨酸、色氨酸和D/L-甲硫氨酸是广泛使用的动物饲料添加剂。(Rehm等人(编辑),生物技术(Biotechnology),第6卷,第14a章中的Leuchtenberger,W.(1996)Amino aids-technicalproduction and use,466-502页,VCH:Weinheim)。还发现这些氨基酸适用作前体,用于合成合成的氨基酸和蛋白质,如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟色氨以及在Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry),A2卷,57-97页,VCH:Weinheim,1985中所述的其它物质。
在可产生这些天然氨基酸的生物体如细菌中已充分描述了它们的生物合成(细菌氨基酸生物合成及其调节的综述,见Umbarger,H.E.(1978)Ann.Rev.Biochem.47:533-606)。谷氨酸通过还原胺化α-酮戊二酸(柠檬酸循环中的中间体)而合成。谷氨酰胺、脯氨酸和精氨酸接下来由谷氨酸产生。丝氨酸的生物合成为三步,由3-磷酸甘油酸(糖酵解中间体)开始,在氧化、氨基转移和水解步骤后产生该氨基酸。半胱氨酸和甘氨酸两者均产生自丝氨酸;具体地,前者通过高半胱氨酸与丝氨酸缩合,后者在丝氨酸转羟甲基酶催化的反应中通过侧链β-碳原子转移至四氢叶酸上合成。苯丙氨酸和酪氨酸在9步的生物合成途径中合成自糖酵解和磷酸戊糖途径的前体赤藓糖4-磷酸和磷酸烯醇丙酮酸,它们的不同仅在预苯酸合成后的最后两步。色氨酸也从这两种起始分子产生,但其合成为11步的途径。酪氨酸也可在苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸均为来自丙酮酸盐(糖酵解的终产物)的生物合成产物。天冬氨酸形成自草酰乙酸(柠檬酸循环的中间体)。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸均通过转化天冬氨酸而形成。异亮氨酸从苏氨酸形成。组氨酸在一个复杂的9步途径中从一种活化糖——5-磷酸核糖-1-焦磷酸形成。
超过蛋白质生物合成所需的量的氨基酸不能被贮藏,并被降解以提供用于细胞主要代谢途径的中间体(综述参见Stryer,L.生物化学(Biochemistry)第三版,21章,“氨基酸降解和尿素循环”495-516页(1988))。虽然细胞可将不想要的氨基酸转化为有用的代谢中间体,但从所需用于合成氨基酸的能量、前体分子和酶来说,氨基酸的产生是昂贵的。因此氨基酸的生物合成受到反馈抑制调节并不让人吃惊,其中特定氨基酸的存在起到减缓或完全终止其自身产生的作用(关于氨基酸生物合成途径中的反馈机制的综述参见Stryer,L.生物化学(Biochemistry)第三版,24章:”氨基酸和血红素的生物合成”575-600页(1988))。因此,特定氨基酸的产出受到细胞中存在的该氨基酸的量的限制。
B.维生素、辅因子和营养成分的代谢和用途
维生素、辅因子和营养成分包含高等动物不能合成且必须摄取的另一类分子,虽然它们易于被其它生物体如细菌合成。这些分子自身为生物活性物质或为在多种代谢途径中作为电子载体或中间体的生物活性物质的前体。除了它们的营养价值外,这些化合物作为着色剂、抗氧化剂和催化剂或其它操作辅助剂也具有重要的工业价值(这些化合物的结构、活性和工业应用的概述参见例如,Ullmann工业化学百科全书(Ullmann’sEncyclopedia of Industrial Chemistry),“维生素”A27卷,443-613页,VCH:Weinheim,1996)。术语”维生素”为本领域认知,包括生物体行使正常功能所需的营养素,且该生物体自身不能合成它们。维生素组可以包括辅因子和营养性化合物。术语“辅因子”包括进行正常酶促活性所需的非蛋白质性化合物。此类化合物可为有机的或无机的,本发明的辅因子优选有机的。术语“营养成分”包括促进植物和动物尤其是人的健康的食物添加剂。此类分子的实例为维生素、抗氧化剂和某些脂类(如多不饱和脂肪酸)。
已大量描述了这些分子在可产生它们的生物体如细菌中的生物合成(Ullmann工业化学百科全书(Ullmann’s Encyclopedia of IndustrialChemistry),“维生素”A27卷,443-613页,VCH:Weinheim,1996;Michal,G.(1999)生物化学途径:生物化学和分子生物学图集(BiochemicalPathways:An Atlas of Biochemistry and Molecular Biology),John Wiley& Sons;Ong,A.S.,Niki,E.和Packer,L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition,Lipids,Health,and Disease”Proceedings of theUNESCO/Confederation of Scientific and Technological Associations inMalaysia,and the Society for Free Radical Research-Asia),1994年9月1-3日在马来西亚槟榔屿举行,AOCS Press:Champaign,IL X,374 S)。
硫胺素(维生素B1)由嘧啶和噻唑部分通过化学偶联产生。核黄素(维生素B2)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素接下来用于合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。合起来称为‘维生素B6’的化合物类(例如,吡哆素、吡哆胺、5’-磷酸吡哆醛和商业上使用的维生素B6盐酸盐)均为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸(R)-(+)-N-(2,4-二羟基-3,3-二甲基-1-氧代丁基)-β-丙氨酸)可通过化学合成或发酵产生。泛酸生物合成的最后步骤由ATP-驱动的β-丙氨酸和泛解酸的缩合组成。负责泛解酸和β-丙氨酸转化及泛酸缩合生物合成步骤的酶是已知的。泛酸盐的代谢活性形式为辅酶A,其生物合成由5步酶促步骤进行。泛酸盐、5’-磷酸吡哆醛、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸盐的形成,而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(维生素B5原)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。
已详细研究了微生物中生物素从前体分子庚二酰辅酶A的生物合成,并鉴定了几种所涉及的基因。发现许多相应的蛋白质也参与
-簇合成,属于蛋白质nitS类。硫辛酸衍生自辛酸,并在能量代谢中作为辅酶,此时其成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸盐为一组这样的物质,其均为叶酸衍生物,而叶酸来自于L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已详细研究了某些微生物中始于鸟苷-5’-三磷酸(GTP)的生物转化的代谢中间体、L-谷氨酸和对氨基苯甲酸的叶酸及其衍生物的生物合成。
类可啉(如钴胺素且尤其是维生素B12)和卟啉属于一组特征在于四吡咯环体系的化学品。维生素B12的生物合成太复杂,目前尚未彻底解释清楚,但许多涉及的酶和底物现已知。烟酸(烟酸盐)和烟酰胺为吡啶衍生物,也称为‘烟酸(niacin)’。烟酸为重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)和它们的还原形式的前体。
这些化合物的工业规模的生产基本上依赖于无细胞的化学合成,虽然这些化学品中的一些如核黄素、维生素B6、泛酸盐和生物素也已通过微生物的大规模培养物产生。只有维生素B12因为其合成的复杂性是仅通过发酵产生的。体外方法需要支出相当大量的材料和时间,并常常代价很高。
C.嘌呤、嘧啶、核苷和核苷酸的代谢和用途
嘌呤和嘧啶代谢基因及它们相应的蛋白质为治疗肿瘤和病毒感染的重要靶标。术语“嘌呤”或“嘧啶”包括构成核酸、辅酶和核苷酸的含氮碱基。术语“核苷酸”包括核酸分子的基本结构单元,其包括含氮碱基、戊糖(对RNA而言,该糖为核糖,对DNA而言,该糖为D-脱氧核糖)和磷酸。术语“核苷”包括作为核苷酸前体的分子,但其不含核苷酸所具有的磷酸单元。通过抑制这些分子的生物合成,或抑制它们形成核酸分子的动员,可抑制RNA和DNA合成;通过以靶向癌细胞的方式抑制这种活性,可抑制肿瘤细胞分裂和复制的能力。
此外,有不形成核酸分子但作为能量贮存体(即AMP)或作为辅酶(即FAD和NAD)的核苷酸。
一些出版物已描述了这些化学品通过影响嘌呤和/或嘧啶代谢在这些医学适应症中的用途(例如Christopherson,R.I.和Lyons,S.D.(1990)”作为化学治疗剂的嘧啶和嘌呤从头生物合成的强力抑制剂”医学研究述评(Med.Res.Reviews)10:505-548)。对参与嘌呤和嘧啶代谢的酶的研究集中在研发可用作如免疫抑制剂或抗增生剂的新药上(Smith,J.L.,(1995)”核苷酸合成中的酶”,结构生物学最新观点(Curr.ODin.Struct.Biol.)5:752-757;(1995)Biochem Soc.Transact.23:877-902)。但是,嘌呤和嘧啶碱基、核苷和核苷酸具有其它可能应用:用作多种精细化学品(例如,硫胺素、S-腺苷-甲硫氨酸、叶酸盐或核黄素)生物合成的中间体,作为细胞的能量载体(例如,ATP或GTP),和本身作为化学品,通常用作增味剂(例如,IMP或GMP)或用于一些医药用途(参见例如,Kuninaka,A.(1996)生物技术中的核苷酸和相关化合物(Nucleotides and Related Compounds inBiotechnology)第6卷,Rehm等人编辑.VCH:Weinheim,561-612页)。同样,参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作靶,研发用于作物保护的抗这些酶的化学品,包括杀真菌剂、除草剂和杀虫剂。
已阐释了这些化合物在细菌中的代谢(综述参见例如,核酸研究和分子生物学进展(Progress in Nucleic Acid Research and Molecular Biology),第42卷,Academic Press中的Zalkin,H.和Dixon,J.E.(1992)”嘌呤核苷酸的从头生物合成”,259-287页;和生物化学途径:生物化学和分子生物学图集(Biochemical Pathways:An Atlas of Biochemistry and MolecularBiology),Wiley:纽约一书中的Michal,G.(1999)”核苷酸和核苷”,第8章)。嘌呤代谢已是深入研究的主题,其对细胞的正常功能是必需的。高等动物中受损的嘌呤代谢可引起严重疾病,如痛风。嘌呤核苷酸从核糖-5-磷酸开始,经过一系列步骤,通过中间体化合物次黄苷-5’-磷酸(IMP),产生鸟苷-5’-单磷酸(GMP)或腺苷-5’-单磷酸(AMP),由此可以容易地形成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存体,这样它们的降解可为细胞内许多不同生物化学过程提供能量。嘧啶的生物合成由核糖-5-磷酸形成尿苷-5’-单磷酸(UMP)而进行。UMP接下来转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式通过一步还原步骤从核苷酸的二磷酸核糖形式产生为核苷酸的二磷酸脱氧核糖形式。通过磷酸化后,这些分子可参与DNA合成。
D.海藻糖的代谢和用途
海藻糖由两个葡萄糖分子通过α、α-1,1键连接组成。它通常作为甜味剂用于食品工业,或作为干的或冷冻食品和饮料中的添加剂。但它也用于药学、化妆品和生物技术工业(参见例如,Nishimoto等人(1998)美国专利号5,759,610;Singer,M.A.和Lindquist,S.生物技术趋势(TrendsBiotech.)16(1998):460-467;Paiva,C.L.A.和Panek,A.D.Biotech.Ann.Rev.2(1996):293-314;和Shiosaka,M.J.Japan 172:(1997)97-102)。海藻糖可以通过许多微生物的酶产生,并天然释放入环境介质中,从中可用本领域公知的方法分离海藻糖。
II.本发明元素和方法
本发明至少部分是基于新分子的检测,它们在本文中被称作MCP核酸分子。这些MCP核酸分子不仅适用于鉴定谷氨酸棒状杆菌或相关细菌种,而且还适于作为标记绘制谷氨酸棒状杆菌基因组图谱,以及鉴定适合如通过发酵工艺生产精细化学品的细菌。本发明还至少部分是基于所述MCP核酸分子编码的MCP-蛋白分子。这些MCP分子能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率,作为谷氨酸棒状杆菌或相关生物体的鉴定标记,降解碳氢化合物和作为研发治疗性药学化合物的靶标。在一个实施方案中,本发明的MCP分子直接或间接地参与谷氨酸棒状杆菌中一种或多种精细化学品的代谢途径。在优选的实施方案中,本发明的MCP分子直接或间接地参与此类代谢途径的行为对所述微生物中目的精细化学品的生产具有影响。在尤其优选的实施方案中,本发明的MCP分子的活性被调节,由此调节本发明的MCP蛋白所参与的谷氨酸棒状杆菌代谢途径的效率或产出,而这又直接或间接地调节谷氨酸棒状杆菌中目的精细化学品的产量或生产效率。
术语“MCP蛋白”或“MCP多肽”包括可调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物以及作为药物筛选或药物设计的靶标蛋白、或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记的蛋白。MCP蛋白的例子包括那些由表1和集合A中列出的MCP基因编码的蛋白。术语“MCP基因”或“MCP核酸序列”包括编码MCP蛋白、含有编码区和相应的非翻译的5’和3’序列区的核酸序列。MCP基因的实例在表1中列出。
术语“产量”和“生产力”是本领域中已知的并且包括在预定的时间段和预定的发酵体积内生产的发酵产物(例如,目标精细化学品)的浓度(例如kg产物/h/l)。术语“生产效率”包括为得到特定产物量所需的时间(例如,细胞为达到精细化学品的特定生产速率所需的时间)。术语“产率”或“产物/碳产率”是本领域已知的并且包括将碳源转化成产物(即精细化学品)的效率。这例如通常表达为kg产物/kg碳源。增加化合物的产率或产量可在预定时间内在特定培养体积中增加所得分子或该化合物的适宜得到的分子的量。术语“生物合成”和“生物合成途径”是本领域中已知的并且包括细胞在例如多步过程或高度调节的过程中从中间物合成化合物,优选有机化合物。术语“降解”和“降解途径”是本领域中已知的并且包括细胞在例如多步过程或高度调节的过程中将化合物,优选有机化合物切割成降解产物(用更通俗的术语:更小或更简单的分子)。术语“代谢”是本领域中已知的并且包括生物体中发生的生物化学反应的整体。因此,特定化合物的代谢(例如氨基酸如甘氨酸的代谢)包括细胞中与该化合物有关的所有生物合成、修饰和降解途径。
在另一个优选的实施方案中,本发明的MCP分子能够直接或间接地调节微生物如谷氨酸棒状杆菌中目的分子如精细化学品的生产。利用基因重组技术,可以操作一种或多种本发明的MCP蛋白使它们的功能受到调节。这种功能调节可以导致对来自谷氨酸棒状杆菌的一种或多种精细化学品的产率、产量和/或生产效率的调节。
例如,可以通过修饰参与精细化学品的生物合成或降解的蛋白的活性(即通过相应基因的突变),直接地调节细胞合成或降解所述化合物的能力,由此调节该精细化学品的产率和/或生产效率。同样,可以通过调节调控精细化学品代谢途径的蛋白的活性直接加以影响,不论期望化合物的产量是上调还是下调,两种情况都将调节来自细胞的精细化学品的产率或生产效率。
精细化学品产量的间接调节也可以通过修饰本发明的蛋白的活性(即通过相应基因的突变),使细胞生长和分裂或者维持存活或生产力的能力总体上得以提高而进行。由谷氨酸棒状杆菌生产精细化学品通常通过在经常是对生长或细胞分裂次最佳的条件下大规模发酵培养所述微生物来实现。修饰本发明的蛋白(如应激反应蛋白、细胞壁蛋白或者与细胞分裂和细胞生长发生所必需的化合物如核苷酸和氨基酸的代谢有关的蛋白)以至于所述条件下细胞的生存、生长和繁殖得以提高,将能够使所述修饰的谷氨酸棒状杆菌细胞在大规模培养中的数量和生产力提高,并且这接下来将提高一种或多种期望精细化学品的产率和/或生产效率。此外,细胞代谢途径必然地彼此相依并被共同调控。改变谷氨酸棒状杆菌任一代谢途径的活性(即改变参与此类途径的本发明蛋白之一的活性),可以同时改变或者调节这个微生物中另一代谢途径的活性,而此另一代谢途径可能直接地涉及精细化学品的合成或降解。
本发明分离的核酸序列定位于可从美国典型培养物保藏中心(American Type Culture Collection)获得的谷氨酸棒状杆菌菌株的基因组中,该菌株的保藏号为ATCC 13032。分离的谷氨酸棒状杆菌MCP核酸分子的核苷酸序列和预测的该谷氨酸棒状杆菌MCP蛋白的氨基酸序列分别如集合A和集合B所示。进行计算分析,将这些核苷酸序列中的许多归类于和/或鉴定为与大肠杆菌(E.coli)或枯草芽孢杆菌(Bacillussubtilis)基因具有同源性的序列。
本发明也涉及这样的蛋白质,其具有实质上与集合B中的氨基酸序列同源的氨基酸序列。如本文所用,其所具有的氨基酸序列与所选氨基酸序列实质上同源的蛋白至少约50%同源于所选氨基酸序列,例如所选完整氨基酸序列。其所具有的氨基酸序列与所选氨基酸序列实质上同源的蛋白也可至少约50-60%,优选地至少约60-70%,且更优选地至少约70-80%、80-90%、或90-95%,且最优选地至少约96%、97%、98%、99%或甚至更高地同源于所选的氨基酸序列。
本发明的MCP蛋白或其生物活性部分或其片段能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率,或降解碳氢化合物,或氧化萜类化合物,或作为药物研发的靶标或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。
下面的小章节更详细地描述了本发明的各个方面:
A.分离的核酸分子
本发明的一个方面涉及编码MCP分子或其生物活性部分的分离核酸分子及核酸片段,其中所述核酸片段足以用作杂交探针或引物,用来鉴定或扩增编码MCP的核酸(例如MCP DNA)。这些核酸分子可用于鉴定谷氨酸棒状杆菌或相关生物体,用于绘制谷氨酸棒状杆菌或相关生物体基因组图谱,或者用于鉴定适于例如通过发酵工艺产生精细化学品的微生物。如此处所用,术语“核酸分子”是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)和用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’和5’端的非翻译区:基因编码区5’端上游的至少约100个核苷酸的序列和基因编码区3’端下游的至少约20个核苷酸的序列。核酸分子可为单链或双链,但优选地为双链DNA。“分离的”核酸分子为从存在于此核酸的天然源中的其它核酸分子中取出的核酸分子。优选地,“分离的”核酸不具有在该核酸所来自的生物体基因组DNA中天然存在于该核酸侧翼的序列(例如,位于该核酸5’或3’端的序列)。例如,在不同实施方案中,该分离的MCP核酸分子可包含少于约5kb,4kb,3kb,2kb,1kb,0.5kb或0.1kb在该核酸所来自的细胞(如谷氨酸棒状杆菌细胞)基因组DNA中天然存在于该核酸分子侧翼的核苷酸序列。而且,“分离的”核酸分子如cDNA分子当通过重组技术产生时可基本上不含有其它细胞物质或培养基,当通过化学合成产生时,可基本上不含有化学前体或其它化学品。
本发明的核酸分子,如具有集合A的核苷酸序列的核酸分子或其部分可用标准的分子生物学技术和本文提供的序列信息分离。例如,使用集合A的完整或部分序列作为杂交探针和标准的杂交技术(例如,在Sambrook,J.,Fritsh,E.F.和Maniatis,T.分子克隆:实验室手册(Molecular Cloning:A Laboratory Manual)。第二版,冷泉港实验室,冷泉港实验室出版社,冷泉港,纽约,1989中所述),可自谷氨酸棒状杆菌文库分离谷氨酸棒状杆菌MCP cDNA。而且,包含集合A的完整序列或其部分的核酸分子可使用根据该序列设计的寡核苷酸引物,应用聚合酶链反应分离(例如,包含集合A的完整或部分序列的核酸分子可使用根据该同样序列产生的寡核苷酸引物,应用聚合酶链反应分离)。例如,可从正常的内皮细胞分离mRNA(例如,通过Chirgwin等人(1979)生物化学(Biochemistry)18:5294-5299的硫氰酸胍提取法),并用反转录酶(例如,莫洛尼鼠类白血病病毒反转录酶,可从Gibco/BRL,Bethesda,MD获得;或AMV反转录酶,可从Seikagaku America,Inc.,St.Petersburg,FL获得)和随机多核苷酸引物或基于集合A中所示的任一核苷酸序列的寡核苷酸引物制备cDNA。用于聚合酶链反应扩增的合成寡核苷酸引物可根据集合A所述的核苷酸序列之一产生。本发明的核酸可用cDNA或用基因组DNA作为模板、使用合适的寡核苷酸引物按照标准的PCR扩增技术来扩增。这样扩增的核酸可克隆入合适的载体并通过DNA序列分析鉴定。对应于MCP核苷酸序列的寡核苷酸还可以通过标准的合成技术制备,例如用DNA自动合成仪制备。
在一个优选的实施方案中,本发明的分离核酸分子包含集合A中所列的核苷酸序列之一。集合A的序列对应于本发明的来自谷氨酸棒状杆菌的MCP cDNA。这些cDNA包含MCP蛋白序列(即在集合A的每一序列中所指出的“编码区”)以及同样在集合A中指出的5’和3’的非翻译区。作为备选方案,核酸分子可以只包含集合A中核苷酸序列之一的编码区。
除此之外,本发明的核酸分子可以只包含集合A中核苷酸序列之一的编码区的一部分,例如可用作探针或引物的片断,或者编码MCP蛋白的生物活性部分的片断。从克隆谷氨酸棒状杆菌MCP基因确定的核苷酸序列使得可产生设计用于鉴定和/或克隆其它细胞类型和生物体中的MCP同系物以及来自其它棒状杆菌或相关种的MCP同系物的探针和引物。探针或引物通常包含基本上纯的寡核苷酸。该寡核苷酸通常包含在严谨条件下可以与集合A中所示的核苷酸序列之一的有义链、集合A中所示的核苷酸序列之一的反义链或其天然存在的突变体中的至少约12个,优选地约25个,更优选地约40、50或75个连续核苷酸杂交的核苷酸序列区域。基于集合A的核苷酸序列的引物可用于PCR反应中以克隆MCP同系物。基于MCP核苷酸序列的探针可用于检测编码相同或同源蛋白的转录本或基因组序列。在优选的实施方案中,该探针还包含与其连接的标记基团,例如放射性同位素、荧光化合物、酶或酶辅因子。这些探针可用作诊断试验试剂盒的一部分用来鉴定误表达MCP蛋白的细胞,例如测定细胞样品中MCP编码核酸的量,例如检测MCP mRNA水平,或确定基因组MCP基因是否已突变或已缺失。
在一个实施方案中,本发明的核酸分子编码这样的蛋白或其部分,其包括的氨基酸序列充分同源于集合B的氨基酸序列,以至于该蛋白或其部分仍然保留调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、作为药物研发的靶标或作为谷氨酸棒状杆菌或相关生物体的鉴定标记的能力。如本文所用,术语“充分同源”是指蛋白或其部分所具有的氨基酸序列包括最小数目的与集合B的氨基酸序列相同或等同的氨基酸残基(例如,氨基酸残基具有与集合B中序列之一的氨基酸残基相似的侧链),以至于该蛋白或其部分能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、作为药物研发的靶标或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。这些活性的实例也在本文进行了描述。因此,“MCP蛋白的功能”有助于一种或多种精细化学品的代谢途径的总体调节,或碳氢化合物的降解,或萜类化合物的氧化。
本发明MCP核酸分子编码的蛋白的部分优选是任何MCP蛋白的生物学活性部分。如本文所用的术语“MCP蛋白的生物学活性部分”包括MCP蛋白的一部分,例如结构域或基元,其能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、以及作为药物研发的靶标或作为谷氨酸棒状杆菌或相关生物体的鉴定标记。为了确定MCP蛋白或者其生物学活性部分能否调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和生产效率、降解碳氢化合物或氧化萜类化合物,可以进行酶活性分析。这些分析方法在实施例部分的实施例8中详细描述,且技术人员熟知这些方法。
其它编码MCP蛋白生物活性部分的核酸片段可通过分离集合B中序列之一的部分、表达所编码的MCP蛋白或MCP肽的部分(例如,通过体外重组表达)并测定所编码的MCP蛋白或MCP肽的部分的活性而制备。
而且,本发明包括由于遗传密码的简并性而不同于集合A所示的核苷酸序列之一(及其部分),并由此与集合A所示的此核苷酸序列编码相同的MCP蛋白的核酸分子。在另一实施方案中,本发明的分离核酸分子具有这样的核苷酸序列,其编码的蛋白具有如集合B中所示的氨基酸序列。在进一步的实施方案中,本发明的核酸分子编码全长的谷氨酸棒状杆菌蛋白,而该蛋白基本上同源于集合B中所示的氨基酸序列(由集合A中所示的可读框编码)。
除了群体中可能存在的MCP序列的天然变体外,技术人员还理解可通过突变向集合A的核苷酸序列中导入变化,由此导致所编码的MCP蛋白的氨基酸序列改变,但不损害MCP蛋白的功能。例如,可在集合A的核苷酸序列中进行在“非必需”氨基酸残基处导致氨基酸替换的核苷酸替换。“非必需”氨基酸残基是任一野生型MCP蛋白序列(集合B)中可以被修饰而不改变所述MCP蛋白的活性的残基,但“必需”氨基酸残基为MCP蛋白活性所需。然而,其它氨基酸残基(例如,在具有MCP活性的结构域中不保守或仅为半保守的那些氨基酸残基)对活性可能为非必需的,并因此可能被改变而不导致MCP活性的改变。
因此,本发明的另一个方面涉及这样的核酸分子,其编码的MCP蛋白含有对于MCP活性非必需的修饰的氨基酸残基。这些MCP蛋白的氨基酸序列与集合B中的序列不同,但尽管如此,该蛋白保留至少一种本文所述的MCP活性。在一个实施方案中,该分离的核酸分子包含编码蛋白的核苷酸序列,其中该蛋白包含至少约50%同源于集合B中的氨基酸序列的氨基酸序列,并且它能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、作为药物研发的靶标或作为谷氨酸棒状杆菌或相关生物体的鉴定标记。
编码同源于集合B的蛋白质序列的MCP蛋白的分离核酸分子可通过将一个或多个核苷酸替换、添加或缺失导入集合A的核苷酸序列中以使一个或多个氨基酸替换、添加或缺失导入所编码的蛋白质中而产生。可通过标准技术将突变导入集合A的核苷酸序列之一,如定点诱变和PCR-介导的诱变。优选地,在一处或多处预测的非必需氨基酸残基处进行保守氨基酸替换。“保守氨基酸替换”为将氨基酸残基用具有相似侧链的氨基酸残基替换。具有相似侧链的氨基酸残基家族已在本领域定义过。这些家族包括具有碱性侧链的氨基酸(例如,赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如,天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如,甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如,丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如,苏氨酸、缬氨酸、异亮氨酸)和具有芳族侧链的氨基酸(例如,酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此,优选地在MCP蛋白中预测的非必需氨基酸残基用来自同一侧链家族的另一氨基酸残基替换。或者,在另一实施方案中,可通过如饱和诱变沿着全部或部分MCP编码序列随机导入突变,并根据文中所述的MCP活性对所得突变体进行检测,以鉴定保留MCP活性的突变体。对集合A序列之任一诱变后,可重组表达编码蛋白,并用例如文中所述的试验(参见实施例部分的实施例8)确定所述蛋白质的活性。
B.重组表达载体和宿主细胞
本发明的另一方面涉及包含编码MCP蛋白(或其部分)的核酸的载体,优选表达载体。如此处所用,术语”载体”是指这样的核酸分子,其可转运与其连接的另一核酸。一种类型的载体为”质粒”,是指其中可连接额外DNA片段的环状双链DNA环。另一种类型的载体为病毒载体,其中可将额外DNA片段连接入病毒基因组。某些载体可在它们所导入的宿主细胞中自主复制(例如,具有细菌复制起点的细菌载体和游离型哺乳动物载体)。其它载体(例如,非游离型哺乳动物载体)在导入宿主细胞后整合入宿主细胞的基因组,并因此随着宿主基因组而复制。而且,某些载体可指导与它们功能性连接的基因的表达。此类载体此处称为”表达载体”。一般,可以用于重组DNA技术中的表达载体为质粒形式。在本说明书中,”质粒”和”载体”可互换使用,因为质粒是最经常使用的载体形式。但是,本发明旨在包括其它形式的表达载体,如病毒载体(例如,复制缺陷型反转录病毒、腺病毒和腺相关病毒),它们起到类似的作用。
本发明的重组表达载体以适于在宿主细胞中表达本发明核酸的形式包含本发明的核酸,这就是说重组表达载体包括一个或多个调节序列,这些调节序列基于表达所用的宿主细胞进行选择并功能性地与待表达的核酸序列连接。在重组表达载体中,术语“功能性连接”是指目的核苷酸序列与调节序列以允许该核苷酸序列表达(例如,在体外转录/翻译系统中,或当载体导入宿主细胞时在宿主细胞中表达)的方式连接。术语“调节序列”旨在包括启动子、阻遏物结合位点、激活物结合位点、增强子区和其它表达调控元件(例如,终止子、mRNA二级结构的其它元件或者多聚腺苷酸化信号)。此类调节序列如在Goeddel;基因表达技术:酶学方法(GeneExpression Technology:Methods in Enzymology)185,Academic Press,SanDiego,CA(1990)中描述。调节序列包括控制核苷酸序列在许多类型宿主细胞中组成型表达的调节序列和控制核苷酸序列仅在特定宿主细胞中表达的调节序列。本领域普通技术人员了解,表达载体的设计可取决于如对欲转化宿主细胞的选择、所需的蛋白质表达程度等因素。本发明的表达载体可导入宿主细胞,以产生由此处所述核酸所编码的蛋白质或肽,包括融合蛋白或融合肽(例如,MCP蛋白、MCP蛋白的突变形式、融合蛋白等)。
本发明的重组表达载体可设计用于在原核或真核细胞中表达MCP蛋白。例如,MCP基因可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母细胞和其它真菌细胞(参见Romanos,M.A.等人(1992)“在酵母中外源基因的表达:综述”(”Foreign gene Expression inyeast:a review”),酵母(Yeast)8:423-488;J.W.Bennet和L.L.Lasure编辑的《更多的用于真菌的基因操作》(More Gene Manipulations inFungi),Academic Press:San Diego一书中的van den Hondel,C.A.M.J.J.等人(1991)“丝状真菌中的异源基因表达”(”Heterologous geneExpression in filamentous fungi”),396-428页;以及Peberdy,J.F.等人编辑的《真菌的应用分子遗传学》(Applied Molecular Genetics of Fungi),Cambridge University Press:Cambridge一书中的van den Hondel,C.A.M.J.J.和Punt,P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfer systems and vector development for filamentous fungi),1-28页)、藻类和多细胞植物细胞(参见Schmidt,R.和Willmitzer,L.(1988),“根癌农杆菌介导的拟南芥叶和子叶外植体的高效转化”,植物细胞报道(Plant Cell Rep.):583-586)或哺乳动物细胞中表达。合适的宿主细胞在Goeddel,基因表达技术:酶学方法(Gene Expression Technology:Methods in Enzymology)185,Academic Press,San Diego,CA(1990)中进一步进行了讨论。备选地,可体外转录并翻译重组表达载体,如使用T7启动子调节序列和T7聚合酶。
在原核生物中蛋白的表达主要用含有组成型或诱导型启动子的载体进行,以控制融合或非融合蛋白的表达。融合载体将一些氨基酸添加至其中编码的蛋白上,通常添加至重组蛋白的氨基末端。这些融合载体一般具有三个任务:1)用于增强重组蛋白的表达;2)用于增加重组蛋白的溶解性;和3)在亲和纯化中作为配体而利于重组蛋白的纯化。通常,将蛋白酶剪切位点于融合单元和重组蛋白的连接处导入到融合表达载体中,这使得在该融合蛋白纯化后,可将重组蛋白与融合单元分离。这些酶和它们的对应识别序列包括Xa因子、凝血酶和肠激酶。
通常的融合表达载体包括pGEX(Pharmacia Biotech Inc;Smith,D.B.和Johnson,K.S.(1988)基因(Gene)67:31-40)、pMAL(New EnglandBiolabs,Beverly,MA)和pRIT5(Pharmacia,Piscataway,NJ),它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白A融合至重组靶蛋白。在一个实施方案中,将MCP蛋白的编码序列克隆入pGEX表达载体,以产生编码融合蛋白的载体,该蛋白从N-末端至C-末端包含GST-凝血酶切割位点-X蛋白。该融合蛋白可以使用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。通过将该融合蛋白用凝血酶切割可得到不与GST融合的重组MCP蛋白。
合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等人(1988)基因(Gene)69:301-315)和pET 11d(Studier等人,基因表达技术:酶学方法(Gene Expression Technology:Methods in Enzymology)185,Academic Press,San Diego,California(1990)60-89)。pTrc载体中靶基因的表达依赖于宿主RNA聚合酶从杂合的trip-lac融合启动子的转录。pET 11d载体中靶基因的表达依赖于共表达的病毒RNA聚合酶(T7 gn1)介导的从T7-gn10-lac融合启动子的转录。该病毒聚合酶可以由宿主菌株BL21(DE3)或HMS174(DE3)中定居的、具有位于lacUV5启动子转录调控下的T7 gn1基因的λ原噬菌体提供。
最大化重组蛋白表达的一个策略是将该蛋白在对所述重组蛋白进行蛋白酶切割的能力被破坏的宿主细菌中表达(Gottesman,S.,基因表达技术:酶学方法(Gene Expression Technology:Methods in Enzymology)185,Academic Press,San Diego,California(1990)119-128)。另一策略是改变欲插入表达载体的核酸的核酸序列,以使编码每一氨基酸的各密码子为选择用于表达的细菌如谷氨酸棒状杆菌所优选使用的密码子(Wada等人(1992)核酸研究(Nucleic Acids Res.)20:2111-2118)。本发明核酸序列的这种改变可应用DNA合成的标准技术实施。
在另一实施方案中,MCP蛋白表达载体为酵母表达载体。用于在酵母菌酿酒酵母(S.cerevisiae)中表达的载体的实例包括pYepSec1(Baldari,等人(1987)Embo J.6:229-234)、pMFa(Kurjan和Herskowitz,(1982)细胞(Cell)30:933-943),pJRY88(Schultz等人(1987)基因(Gene)54:113-123)和pYES2(Invitrogen Corporation,San Diego,CA)。适用于其它真菌如丝状真菌的载体和用于构建载体的方法包括在:J.F.Peberdy等人编辑的《真菌的应用分子遗传学》(Applied Molecular Genetics of Fungi),CambridgeUniversity Press:Cambridge一书中的van den Hondel,C.A.M.J.J.和Punt,P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfersystems and vector development for filamentous fungi),1-28页中详述的那些。
作为另一备选方案,本发明的MCP蛋白可使用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf 9细胞)中表达蛋白的杆状病毒载体包括pAc系列(Smith等人(1983)Mol.Cell Biol.3:2156-2165)和pVL系列(Lucklow和Summers(1989)病毒学(Virology)170:31-39)。
在另一实施方案中,本发明的MCP蛋白可在单细胞植物细胞(如藻类)或在来自高等植物(如种子植物,诸如作物)的植物细胞中表达。植物表达载体的实例包括在:Becker,D.,KemPer,E.,Schell,J.和Masterson,R.(1992)“具有靠近左边界的选择性标记的新植物双元载体”(”New plantbinary vectors with selectable markers located proximal to the leftborder”),植物分子生物学(Plant Mol.Biol.)20:1195-1197;和Bevan,M.W.(1984)“用于植物转化的双元农杆菌载体”(”Binary Agrobacteriumvectors for plant transformation”),核酸研究(Nucl.Acid.Res.)12:8711-8721中详述的那些。
在另一实施方案中,本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed,B.(1987)自然(Nature)329:840)和pMT2PC(Kaufman等人(1987)EMBO J.6:187-195)。当用于哺乳动物细胞时,表达载体的调控功能通常由病毒调控元件提供。例如,常用的启动子来自多瘤病毒、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它合适表达系统参见Sambrook,J.,Fritsh,E.F.和Maniatis,T.分子克隆:实验室手册(Molecular Cloning:ALaboratory Manual),第二版,Cold Spring Harbor Laboratory,ColdSpring Harbor Laboratory Press,Cold Spring Harbor,NY,1989中的第16和17章。
在另一实施方案中,重组哺乳动物表达载体可以指导核酸优选地在特定细胞类型中表达(例如,使用组织-特异性调控元件来表达核酸)。组织-特异性调控元件为本领域公知。合适的组织-特异性启动子的非限制性实例包括白蛋白启动子(肝特异性;Pinkert等人(1987)基因进展(Genes Dev.)1:268-277)、淋巴特异性启动子(Calame and Eaton(1988)Adv.Immunol.43:235-275),特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8:729-733)和免疫球蛋白启动子(Banerji等人(1983)细胞(Cell)33:729-740;Queen和Baltimore(1983)细胞(Cell)33:741-748)、神经元特异性启动子(例如,神经丝启动子;Byrne和Ruddle(1989)PNAS86:5473-5477)、胰特异性启动子(Edlund等人(1985)科学(Science)230:912-916)以及乳腺特异性启动子(例如,milk serum启动子;美国专利No.4,873,316和欧洲专利申请文件No.264,166)。同样,还包括发育调节的启动子,如鼠hox启动子(Kessel和Gruss(1990)科学(Science)249:374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)基因进展(Genes Dev.)3:537-546)。
而且,本发明提供了含本发明的DNA分子的重组表达载体,其中该DNA分子以反义方向克隆在表达载体中。也就是,DNA分子可操作地连接到调控序列上,以至于可以表达(通过转录该DNA分子)产生与MCPmRNA反义的RNA分子。可以对功能地连接反义方向克隆的核酸的调控序列进行选择以控制反义RNA分子在多种细胞类型中持续表达;例如,可选择病毒启动子和/或增强子或调控序列以控制反义RNA的组成型组织特异性或细胞类型特异性表达。反义表达载体的形式可为重组质粒、噬菌粒或减毒病毒,其在高效调控区的控制下产生反义核酸,而所述调控区的活性由载体所导入的细胞的类型决定。关于利用反义基因实施基因表达调控的讨论见Weintraub,H.等人,反义RNA作为遗传分析的分子工具,Reviews-Trends in Genetics,Vol.1(1)1986。
本发明的另一方面涉及已导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此处可互换使用。当然,该术语不仅指特定的靶细胞,也指该细胞的后代或潜在后代。由于突变或环境因素细胞在传代过程中可发生某些改变,这样,后代可能并不一定与亲代细胞相同,但其仍包括在此处所用术语的范围之内。
宿主细胞可为原核或真核细胞。例如,MCP蛋白可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢(CHO)细胞或COS细胞)中表达。其他合适的宿主细胞为技术人员公知。与谷氨酸棒状杆菌有关的、能够以适宜的方式用作本发明核酸和蛋白分子的宿主细胞的微生物如表3所示。
载体DNA可通过常规转化或转染技术导入原核或真核细胞中。如本文所用,术语“转化”和“转染”、“接合”和“转导”旨在包括本领域公知的各种将外源核酸(例如DNA)导入宿主细胞的技术,包括天然感受态、化学介导的转移、磷酸钙或氯化钙共沉淀、DEAE-葡聚糖-介导的转染、脂转染或电穿孔。转化或转染宿主细胞的合适方法可在Sambrook等人(分子克隆:实验室手册(Molecular Cloning:A Laboratory Manual)。第二版,ColdSpring Harbor Laboratory,Cold Spring Harbor Laboratory Press,ColdSpring Harbor,NY,1989)和其它的实验室手册中找到。
对于哺乳动物细胞的稳定转染,已知根据所用的表达载体和所用的转染技术,仅一小部分的细胞可将外源DNA整合到基因组中。一般将编码选择标记(如抗生素抗性)的基因与目的基因一同导入宿主细胞以鉴定和筛选这些整合体。优选的选择标记包括那些可赋予药物抗性的基因,所述药物如G418、潮霉素和氨甲蝶呤。编码选择标记的核酸可与编码MCP蛋白的基因位于同一载体上被导入宿主细胞或可以位于不同的载体上导入。由导入的核酸稳定转染的细胞可通过例如药物选择进行鉴定(例如,整合有选择标志基因的细胞将存活,而其它细胞死亡)。
通过制备出含至少MCP基因一部分的载体——所述MCP基因一部分中已经导入缺失、添加或替代因而可以改变MCP基因(如功能性破坏)——可以生成同源重组微生物。优选地该MCP基因为谷氨酸棒状杆菌MCP基因,但也可以利用来自相关细菌或甚至来自哺乳动物、酵母或昆虫来源的同系物。在优选的实施方案中,载体设计为在同源重组后可功能性破坏内源MCP基因(即,该基因不再编码功能蛋白;同样地也称为“敲除”载体)。作为备选方案,载体可设计为在同源重组后使内源MCP基因发生突变或以其它方式改变但仍编码功能蛋白(例如,可改变位于上游的调控区由此改变内源MCP蛋白的表达)。在此同源重组载体中,改变的MCP基因部分在其5′和3′端侧翼为MCP基因的其它核酸,从而使得载体所携带的外源MCP基因和微生物中的内源MCP基因之间可以发生同源重组。此额外的侧翼MCP核酸的长度应足以使与内源基因的同源重组成功进行。通常,载体内包括不到一千碱基的侧翼DNA(5′及3′端)(参见例如,Thomas,K.R.,和Capecchi,M.R.(1987)细胞(Cell)51:503中对同源重组载体的描述)。将该载体导入微生物(例如,通过电穿孔),并使用本领域公知的方法,筛选内源MCP基因已与导入的MCP基因同源重组的细胞。
在另一实施方案中,产生的重组微生物含有可调控导入的基因的表达的选定系统。例如,将MCP基因插入载体中位于乳糖操纵子的控制下,可以使MCP基因仅在存在IPTG时表达。这些调控系统为本领域熟知。
本发明的宿主细胞如培养的原核或真核宿主细胞可用于产生(即表达)MCP蛋白。而且,本发明还提供了使用本发明的宿主细胞来产生MCP蛋白的方法。在一个实施方案中,该方法包括在合适的培养基中培养本发明的宿主细胞(其中已导入了编码MCP蛋白的重组表达载体,或其基因组中已导入了编码野生型或改变的MCP蛋白的基因),直至产生MCP蛋白。在另一实施方案中,该方法还包括从培养基或宿主细胞分离MCP蛋白。
C.本发明的用途和方法
文中所述的核酸分子、蛋白、蛋白同系物、融合蛋白、引物、载体和宿主细胞可用于下述一种或多种方法中:鉴定谷氨酸棒状杆菌及相关生物体,绘制与谷氨酸棒状杆菌相关的生物体的基因组图谱,鉴定并定位目的谷氨酸棒状杆菌序列,进化研究,确定MCP蛋白中功能所需的MCP蛋白区域,调节MCP蛋白的活性,调节一种或多种代谢途径的活性,以及调节目的化合物如精细化学品的细胞生产。本发明的MCP核酸分子具有多种用途。首先,它们可用于鉴定生物体是否为谷氨酸棒状杆菌或其近亲。它们也可用于在混合的微生物群体中鉴定谷氨酸棒状杆菌或其相关菌。本发明提供了许多谷氨酸棒状杆菌基因的核酸序列。通过将从微生物单一群体或混合群体的培养物提取的基因组DNA在严谨条件下用包含对谷氨酸棒状杆菌而言独特的谷氨酸棒状杆菌基因区域的探针来探测,可确定该生物体是否存在。虽然谷氨酸棒状杆菌自身为非致病的,但它与致病种如白喉棒状杆菌有亲缘关系。这种生物体的检测具有实质上的临床重要性。
通过利用本领域公知的技术可检测样品中的谷氨酸棒状杆菌。特别地,可以首先在合适的液体或合适的固体培养基中培养样品中的细胞,以增加培养物中细胞的数目。裂解这些细胞并提取其中所含的全部DNA,并且在适当的时候,进行纯化以除去可能干扰随后分析的细胞碎片和蛋白物质。实施本领域公知的聚合酶链式反应或相似技术(有关核酸序列扩增通常所用的方法学的一般概述,参见Mullis等人,美国专利No.4683195,Mullis等人,美国专利No.4965188以及Innis,M.A.,und Gelfand,D.H.(1989)PCR方法,方法及应用指南(PCR-Protocols,A guide to Methods andApplications),Academic Press,pp.3-12,和(1988)生物技术(Biotechnology)6:1197,以及国际专利申请No.WO89/01050),其中,由对本发明的MCP核酸分子特异的引物与样品核酸温育,从而使所述的特定MCP核酸序列,如果存在于样品中的话,得以扩增。待扩增的特定核酸序列的选择依据是它唯一性地存在于谷氨酸棒状杆菌以及仅有的少数近亲细菌的基因组中。期望的扩增产物的存在可指示谷氨酸棒状杆菌或与谷氨酸棒状杆菌密切相关的生物体的存在。
本发明的核酸和蛋白分子也可用作基因组特定区域的标记。利用本领域公知的技术,可以检测本发明的MCP核酸分子在谷氨酸棒状杆菌基因组中的物理定位,而这接下来可用来更容易地在图谱中定位其它核酸分子和基因。而且,本发明的核酸分子可能与相关种的序列充分同源,以至于这些核酸分子同样能够在此类细菌(如乳糖发酵短杆菌(BrevibacteriumLactofermentum))中构建基因组图谱。
本发明的核酸和蛋白分子不仅适于绘制基因组图谱,也适用于谷氨酸棒状杆菌蛋白的功能研究。例如,通过切割谷氨酸棒状杆菌基因组,并将片段与DNA-结合蛋白温育,可鉴定与特定的谷氨酸棒状杆菌DNA-结合蛋白结合的基因组区域。另外,那些结合蛋白的片段可用本发明的核酸分子探测,优选地使用易于检测的标记物;此核酸分子与基因组片段的结合可将片段定位于谷氨酸棒状杆菌的基因组图谱中,并且当用不同的酶重复此过程几次时,将有利于快速确定蛋白结合的核酸序列。
本发明的MCP核酸分子同样也适用于进化研究和蛋白结构研究。许多原核和真核细胞应用本发明分子参与的代谢过程;通过将本发明核酸分子的序列与那些来自其它生物体的编码类似酶的序列进行比较,可确定所述生物体的进化关系。由此,此比较使得可确定哪些序列区域保守以及哪些区域不保守,而这有利于确定对酶功能所必需的那些蛋白区域。这种确定对蛋白工程研究有价值,并可指示蛋白可耐受何种水平的诱变而不丧失其功能。
本发明的MCP蛋白可用作为标记将未知细菌归类为谷氨酸棒状杆菌或鉴定样品中的谷氨酸棒状杆菌或近亲细菌。例如,利用本领域公知的技术,在适当的时候,可以扩增样品中的细胞(例如通过在合适的培养基中培养),以增加样品的大小,然后裂解所述细胞,由此释放其中所含的蛋白。在适当的时候,该样品可以进行纯化,以除去可能会干扰随后的分析的细胞碎片和核酸分子。选定的本发明的MCP蛋白的特异性抗体可以在典型的Western试验形式中与蛋白样品进行温育(例如参见Ausubel等人,(1988)分子生物学最新方法(Current Protocols in Molecular Biology),Wiley:纽约),这样如果所述蛋白存在于样品中的话,抗体与它的靶蛋白结合。如果一个MCP蛋白对谷氨酸棒状杆菌或谷氨酸棒状杆菌及其亲缘关系很近的细菌而言是独特的或几乎是独特的,则该MCP蛋白被选择用于此类试验。样品中的蛋白随后通过凝胶电泳分级分离,并转移到合适的基质如硝酸纤维素上。带有可检测的标记(如化学发光或显色的)的合适的二抗与该基质温育,接着进行严格洗涤。标记的存在或不存在指示了样品中靶蛋白的存在或不存在。如果蛋白存在,则这表明谷氨酸棒状杆菌的存在。相似的方法使得可以将未知细菌归类为谷氨酸棒状杆菌;如果许多谷氨酸棒状杆菌特异性的蛋白在从未知细菌制备的蛋白样品中均未检测到,则该细菌很可能不是谷氨酸棒状杆菌。
对本发明MCP核酸分子的遗传操作可导致产生与野生型MCP蛋白功能不同的MCP蛋白。这些蛋白可在功效或活性上提高,可以在细胞内以比通常多的量存在,或可以在功效或活性上降低。
这些活性的改变可以直接调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率。例如,有可能通过修饰参与精细化学品的生物合成或降解的蛋白的活性(即通过相应基因的突变),直接调节细胞合成或降解所述化合物的能力,由此调节该精细化学品的产率和/和生产效率。同样地,有可能通过调节调控精细化学品代谢途径的蛋白的活性直接加以影响,不论期望化合物的产生是上调还是下调,两种方式都将调节来自细胞的精细化学品的产率或生产效率。
精细化学品产生的间接调节也可以通过修饰本发明的蛋白的活性(即通过相应基因的突变),使细胞生长和分裂或维持存活或生产力的能力总体上得以提高而进行。从谷氨酸棒状杆菌生产精细化学品通常通过在经常是对生长和细胞分裂次最佳的条件下大规模发酵培养所述微生物来实现。修饰本发明的蛋白(如应激反应蛋白、细胞壁蛋白或者与细胞分裂或细胞生长发生所必需的化合物如核苷酸和氨基酸的代谢有关的蛋白)以至于所述条件下细胞的生存、生长和繁殖得以提高,将能够使所述修饰的谷氨酸棒状杆菌细胞在大规模培养中的数量和生产力提高,并且这接下来将提高一种或多种期望的精细化学品的产率和/或生产效率。此外,细胞代谢途径必然地彼此相依并被共的调控。改变谷氨酸棒状杆菌任一代谢途径的活性(即改变参与此类途径的本发明蛋白之一的活性),能够同时改变或者调节这个微生物中另一代谢途径的活性,而此另一代谢途径可能直接地涉及精细化学品的合成或降解。
前述的这些突变MCP蛋白以增加谷氨酸棒状杆菌中精细化学品的产率的策略并不旨在限制;这些突变策略的变通方案对技术人员是相当明显的。使用这些策略且包括本文公开的机制,使得可以使用本发明的核酸和蛋白分子产生表达突变的MCP核酸和蛋白分子的谷氨酸棒状杆菌或者相关细菌菌株,从而提高目的化合物的产率、产量和/或生产效率。目的化合物可以是谷氨酸棒状杆菌的产物,包括生物合成途径的终产物和天然代谢途径的中间产物以及非天然存在于谷氨酸棒状杆菌代谢中的但是被本发明谷氨酸棒状杆菌菌株生产的分子。
以下实施例进一步举例阐释本发明,其不当理解为限制性的。在本专利申请中引用的所有参考文献、专利申请、专利、公开的专利申请的全部内容特此引用作为参考。
实施例
实施例1:谷氨酸棒状杆菌ATCC13032的总基因组DNA的制备
谷氨酸棒状杆菌(ATCC13032)的培养物在30℃于BHI培养基(Difco)中剧烈振摇过夜。离心收获细胞,弃上清并将细胞重悬于5ml缓冲液I(培养物原初体积的5%—所有显示的体积为按100ml培养物体积计算所得)。缓冲液I的组成:140.34g/l蔗糖,2.46g/l
,10ml/l
溶液(100g/l,用
调节至pH6.7),50ml/l M12浓缩物(10g/l
,1g/l
,2g/l
,0.2g/l
,0.5g/l酵母提取物(Difco),10ml/l痕量元素混合物(200mg/l
,10mg/l
,3mg/l
,30mg/l
,20mg/l
,1mg/l
,3mg/l
,500mg/l络合剂(EDTA或柠檬酸),100ml/l维生素混合物(0.2mg/l生物素,0.2mg/l叶酸,20mg/l对氨基苯甲酸,20mg/l核黄素,40mg/l泛酸钙,140mg/l烟酸,40mg/l维生素B6盐酸盐,200mg/l肌醇)。向悬液中加入溶菌酶至终浓度2.5mg/ml。在37℃温育约4小时后,细胞壁降解,并离心收获所得的原生质体。沉淀用5ml缓冲液I洗一次并用5ml TE缓冲液(10mM
,1mM EDTA,pH值8)洗一次。将沉淀重悬于4mlTE缓冲液,并加入0.5ml SDS溶液(10%)和0.5ml
溶液(5M)。加入蛋白酶K至终浓度200μg/ml后,将悬液在37℃温育约18小时。使用标准方法用酚、酚/氯仿/异戊醇和氯仿/异戊醇提取而纯化DNA。然后,加入1/50体积的3M醋酸钠和2体积的乙醇,于-20℃温育30分钟,并在高速离心机上使用SS34转子(Sorvall)于12,000rpm离心30分钟,沉淀DNA。将该DNA溶于含20μg/ml RNaseA的1ml TE缓冲液中,并用1000ml TE缓冲液于4℃透析至少3小时。在此期间,更换缓冲液3次。向分装的0.4ml透析过的等分DNA溶液中加入0.4ml 2M
和0.8ml乙醇。于-20℃温育30分钟后,离心(13,000rpm,Biofuge Fresco,Heraeus,Hanau,德国)收集DNA。将该DNA沉淀溶于TE缓冲液。用此方法制备的DNA可用于所有目的,包括Southern印迹和构建基因组文库。
实施例2:在大肠杆菌中构建谷氨酸棒状杆菌(ATCC13032)的基因组文库
使用如实施例1所述制备的DNA,按照公知的且已建立的方法(参见例如,Sambrook,J.等人(1989)“分子克隆:实验室手册”,冷泉港实验室出版社,或Ausubel,F.M.等人(1994)“分子生物学最新方法”,John Wiley &Sons.)构建粘粒和质粒文库。
可以使用任何质粒或粘粒。特别优选使用的是质粒pBR322(Sutcliffe,J.G.(1979)美国国家科学院院报(Proc.Natl.Acad.Sci.USA)75:3737-3741)、pACYC177(Change和Cohen(1978)细菌学杂志(J.Bacteriol)134:1141-1156)、pBS系列的质粒(pBSSK+,pBSSK-及其它;Stratagene,LaJolla,USA)、或粘粒如SuperCos1(Stratagene,LaJolla,美国)或Lorist6(Gibson,T.J.,Rosenthal A.和Waterson,R.H.(1987)基因(Gene)53:283-286。
实施例3:DNA序列测定和计算机功能分析
如实施例2所述的基因组文库按照标准方法用于DNA序列测定,尤其是使用ABI377序列测定仪通过链终止法测定(参见例如,Fleischman,R.D.等人(1995)“流感嗜血菌(Haemophilus Influenzae)Rd的全基因组随机序列测定和组装”,科学(Science),269:496-512)。使用具有以下核苷酸序列的测序引物:5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。
实施例4:体内诱变
谷氨酸棒状杆菌的体内诱变可通过将质粒(或其它载体)DNA在不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如芽孢杆菌属种或酵母如酿酒酵母)中传代而实施。通常的突变菌株在用于DNA修复系统的基因中有突变(例如mutHLS、mutD、mutT等,对照参见Rupp,W.D.(1996),《大肠杆菌和沙门氏菌》,ASM:Washington一书中的DNA修复机制,2277-2294页)。这些菌株为技术人员熟知。这些菌株的用途在例如Greener,A.和Callahan,M.(1994)Strategies 7:32-34中进行了阐述。
实施例5:在大肠杆菌和谷氨酸棒状杆菌之间的DNA转移
几种棒状杆菌和短杆菌物种含有自主复制的内源质粒(例如pHM1519或pBL1)(综述参见例如,Martin,J.F.等人(1987)生物技术(Biotechnology),5:137-146)。使用标准的大肠杆菌载体可以容易地构建用于大肠杆菌和谷氨酸棒状杆菌的穿梭载体(Sambrook,J.等人(1989),“分子克隆:实验室手册”,冷泉港实验室出版社,或Ausubel,F.M.等人(1994)“分子生物学最新方法”(”Current Protocols in Molecular Biology”),John Wiley & Sons),其中加入用于谷氨酸棒状杆菌中的复制起点和来自谷氨酸棒状杆菌的适当标记。此类复制起点优选取自从棒状杆菌和短杆菌分离的内源质粒。对于这些物种,作为转化标记尤其有用的是卡那霉素抗性基因(如源自Tn5或Tn903转座子的基因)或氯霉素抗性基因(Winnacker,E.L.(1987)“从基因到克隆-基因技术简介”(“From Genes to Clones-Introduction to GeneTechnology”),VCH,Weinheim)。在文献中有许多在大肠杆菌和谷氨酸棒状杆菌中复制的多种穿梭载体的构建例子,这些载体可用于多种目的,包括基因过表达(参见例如,Yoshihama,M.等人(1985)细菌学杂志(J.Bacteriol.)162:591-597,Martin J.F.等人(1987)生物技术(Biotechnology),5:137-146和Eikmanns,B.J.等人(1991)基因(Gene),102:93-98)。
使用标准技术,可将目的基因克隆入上述的穿梭载体之一中并可将此杂合载体导入谷氨酸棒状杆菌菌株。谷氨酸棒状杆菌的转化可通过原生质体转化(Kastsumata,R.等人(1984)细菌学杂志(J.Bacteriol.)159,306-311)、电穿孔(Liebl,E.等人(1989)FEMS Microbiol.Letters,53:399-303)以及当使用特别的载体时,也可通过接合(如在Sch_fer,A等人(1990)细菌学杂志(J.Bacteriol.)172:1663-1666中所述)来完成。同样也可通过制备谷氨酸棒状杆菌的质粒DNA(使用本领域熟知的标准方法)并将其转化至大肠杆菌,将谷氨酸棒状杆菌的穿梭载体转移至大肠杆菌中。该转化步骤可使用标准方法进行,但有利的是使用Mcr-缺陷的大肠杆菌菌株,如NM522(Gough和Murray(1983)分子生物学杂志(J.Mol.Biol.)166:1-19)。
实施例6:测定突变蛋白的表达
在转化的宿主细胞中对突变蛋白活性的观察依赖于突变蛋白与野生型蛋白以相似的方式和相似的量表达的事实。确定突变基因的转录的量(指示可用于翻译为基因产物的mRNA量)的适宜方法是进行Northern印迹(参考文献见例如,Ausubel等人(1988)分子生物学最新方法(Current Protocolsin Molecular Biology),Wiley:纽约),其中设计为与目的基因结合的引物用可检测标志(通常为放射性的或化学发光的)标记,这样当提取生物体培养物的总RNA、在凝胶上分级分离、转移至稳定的基质上并与该探针温育后,探针的结合和结合量将指示该基因的mRNA的存在和存在量。该信息指示了突变基因的转录程度。通过多种本领域公知的方法可从谷氨酸棒状杆菌中制备总细胞RNA,如在Bormann,E.R.等人(1992)分子微生物学(Mol.Microbiol.)6:317-326中所描述的。
从所述mRNA翻译的蛋白的存在或相对量,可使用标准技术如Western印迹(参见例如,Ausubel等人(1988)分子生物学最新方法(Current Protocols in Molecular Biology),Wiley:New York)测定。在该方法中,提取细胞总蛋白、通过凝胶电泳分离、转移至基质如硝化纤维素上并与能和目的蛋白特异性结合的探针如抗体一起温育。该探针通常用易于检测到的化学发光或显色标记物标记。所观察到的标记物的存在和量将指示在细胞中目的突变蛋白的存在和量。
实施例7:遗传改变的谷氨酸棒状杆菌的生长—培养基和培养条件
遗传修饰的棒状杆菌培养在合成或天然的生长培养基中。用于棒状杆菌的许多种不同生长培养基为人们熟知并易于获得(Lieb等人(1989)Appl.Microbiol.Biotechnol.,32:205-210;von der Osten等人(1998)Biotechnology Letters,11:11-16;专利DE 4,120,867;在《原核生物》(TheProcaryotes),Volume II,Balows,A.等人编辑.Springer-Verlag一书中的Liebl(1992)“棒状杆菌属”(“The Genus Corynebacteria”))。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源是糖类,如单糖、二糖或多糖。非常好的碳源的实例是葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可通过复杂化合物如糖蜜或其它来自糖加工的副产品向培养基中提供糖。加入不同碳源的混合物也可能是有利的。其它可能的碳源为醇类和有机酸,如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物,或为包含这些化合物的物质。示例性氮源包括氨气和胺盐如
或
、
、硝酸盐、尿素、氨基酸和复杂氮源如玉米浆、大豆粉、大豆蛋白、酵母提取物、肉膏等。
可包括在培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、磷盐或硫酸盐。可向培养基中加入螯合剂以维持溶液中的金属离子。尤其有用的螯合剂包括二羟基酚,如儿茶酚或原儿茶酸盐,和有机酸如柠檬酸。培养基一般也包括其它生长因子如维生素或生长促进剂,其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和维生素B6。生长因子和盐常来自复杂的培养基组分,如酵母提取物、糖蜜、玉米浆等。培养基的确切组成强烈取决于具体实验并在每一具体情况下单独确定。有关培养基优化的信息在教科书“Applied Microbiol.Physiology,A Practical Approach”(P.M.Rhodes,P.F.Stanbury编辑,IRL Press(1997)53-73页,ISBN 0 19 963577 3)中可找到。也可从供应商处得到生长培养基,如Standard 1(Merck)或BHI(Brain heart infusion,DIFCO)等。
通过加热(1.5巴,121℃加热20分钟)或过滤除菌对所有的培养基组成成份进行灭菌。组成成份可一起灭菌,或需要的话分开灭菌。所有的培养基组成成份可在培养开始时即存在,或它们可按需要连续加入或分批加入。
对每一实验分别确定培养条件。温度应在15℃和45℃的范围之间。在实验中温度可保持恒定或可改变。培养基的pH应在5至8.5的范围之间,优选地在7.0附近,可通过向培养基中添加缓冲液而维持。用于此目的的示例性缓冲液为磷酸钾缓冲液。可备选地或同时地使用合成的缓冲液如MOPS、HEPES、ACES和其它。在生长过程中通过加入
或 也可维持恒定的培养pH。如果使用复杂的培养基组分如酵母提取物,可减轻对额外的缓冲液的需要,因为许多复杂化合物具有高缓冲容量。如果使用发酵罐培养微生物,用气态氨也可调节pH。
培养时间通常在数小时到数天之间。选择培养时间以允许肉汤中累积最大量的产物。可在多种容器如不同规格的微滴定板、玻璃管、玻璃烧瓶或玻璃或金属发酵罐中实施所公开的生长实验。对于筛选大量的克隆,应在微滴定板、玻璃管或在有或没有挡板的摇瓶中培养微生物。优选地使用100ml摇瓶,其中装有10%(体积)的所需生长培养基。该烧瓶应在旋转振荡器(振幅25mm)上以100-300rpm的速度摇动。可通过维持潮湿环境减少蒸发损失;备选地,对蒸发损失应进行数学上的校正。
如果研究经遗传修饰后的克隆,也应分析未修饰的对照克隆或含有无任何插入的基本质粒的对照克隆。使用已于30℃温育的在琼脂板如CM板(10g/l葡萄糖,2.5g/l
,2g/l尿素,10g/l聚胨,5g/l酵母抽取物,5g/l肉膏,22g/l琼脂,用2M
调节至pH6.8)上生长的细胞接种培养基至OD600 0.5-1.5。培养基的接种通过导入来自CM板的谷氨酸棒状杆菌细胞的盐水溶液或添加所述细菌的液体预培养物而完成。
实施例8:突变蛋白功能的体外分析
酶活性和动力学的确定是本领域熟知的。为了确定特定的修饰的酶的活性,实验应加以改良以适应野生型酶的比活性,这在本领域普通技术人员的能力范围内。例如可在下列参考文献中找到对酶的一般性总结以及有关结构、动力学、原理、方法、应用和多种酶活性的测定例子的具体细节:Dixon,M.和Webb,E.C.,(1979)酶(Enzymes),Longmans,伦敦;Fersht,(1985)酶结构和机制(Enzyme Structure and Mechanism),Freeman,纽约;Walsh,(1979)酶促反应机制(Enzymatic Reaction Mechanisms),Freeman,旧金山;Price,N.C.,Stevens,L.(1982)酶学基础(Fundamentalsof Enzymology),牛津大学出版社:Oxford;Boyer,P.D.编辑(1983)酶(TheEnzymes),第三版,Academic Press:纽约;Bisswanger,H.,(1994)酶动力学(Enzymkinetik),第二版.VCH:Weinheim(ISBN 3527300325);Bergmeyer,H.U.,Bergmeyer,J.,Graβl,M.编辑(1983-1986)酶分析方法(Methods of Enzymatic Analysis),第三版,I-XII卷,Verlag Chemie:Weinheim;以及Ullmann工业化学百科全书(Ullmann’s Encyclopedia ofIndustrial Chemistry)(1987)A9卷,”酶”.VCH:Weinheim,352-363页。
可通过几种已建立的方法测定结合DNA的蛋白的活性,如DNA条带移位分析(也称为凝胶阻滞试验)。这些蛋白对其它分子表达的作用可使用报告基因试验测定(如在Kolmar,H.等人(1995)EMBO J.14:3895-3904及其中所引用的参考文献中所述)。报告基因测试系统为人们所熟知,并被建立以应用于原核和真核细胞,其中使用酶如β-半乳糖苷酶、绿色荧光蛋白和其它几种酶。
可根据如在《生物膜,分子结构和功能)》(Biomembranes,MolecularStructure and Function),Springer:Heidelberg一书中的Gennis,R.B.(1989)”孔、通道和转运蛋白”,85-137;199-234和270-322页中所述的技术进行膜转运蛋白活性的测定。
实施例9:分析突变蛋白对目的产物产生的影响
谷氨酸棒状杆菌中的遗传修饰对目的化合物(如氨基酸)产生的影响可通过如下方式确定:将经修饰的微生物在合适的条件(如上文中所描述的那些条件)下生长,并针对目的产物(即氨基酸)产生的增加来分析培养基和/或细胞成分。此类分析技术为本领域普通技术人员熟知,包括光谱学、薄层色谱法、多种显色法、酶学和微生物学方法、以及分析色谱法如高效液相色谱法(参见例如,Ullnan工业化学百科全书(Ullman,Encyclopediaof Industrial Chemistry),A2卷,89-90和443-613页,VCH:Weinheim(1985);Fallon,A.等人(1987)《生物化学和分子生物学实验室技术》(LaboratoryTechniques in Biochemistry and Molecular Biology)中的“HPLC在生物化学中的应用”,17卷;Rehm等人(1993)生物技术(Biotechnology),3卷,III章“产物回收和纯化”,469-714页,VCH:Weinheim;Belter,P.A.等人(1988)生物分离:生物技术中的下游加工(Bioseparations:downstreamprocessing for Biotechnotogy),John Wiley and Sons;Kennedy,J.F.和Cabral,J.M.S.(1992)生物物质的回收方法(Recovery processes forbiological materials),John Wiley and Sons;在《Ulmann工业化学百科全书》一书中的Shaeiwitz,J.A.和Henry,J.D.(1988)生物化学分离,B3卷,11章,1-27页,VCH:Weinheim;以及Dechow,F.J.(1989)生物技术中的分离和纯化技术(Separation and purification techniques in biotechnology),Noyes Publications.)。
除了测定发酵终产物外,同样还可分析用于产生目的化合物的代谢途径的其它成分如中间体和副产物来确定化合物的总体生产效率。分析方法包括测定培养基中的营养素水平(例如,糖类、碳氢化合物、氮源、磷酸盐和其它离子)、测定生物量组成和生长、分析生物合成途径中共同代谢物的产生及测定发酵过程中产生的气体。这些测定的标准方法在《应用微生物生理学,实用方法》(Applied Microbial Physiology,A Practical Approach),P.M.Rhodes和P.F.Stanbury编辑,IRL Press,103-129;131-163和165-192页(ISBN:0199635773)及其引用的参考文献中描述。
实施例10:从谷氨酸棒状杆菌培养物中纯化目的产物
可通过多种本领域熟知的方法从谷氨酸棒状杆菌细胞或上述培养物上清回收目的产物。如果目的产物不从细胞向外分泌,可低速离心从培养物中收获细胞、用标准技术如机械力或超声裂解细胞。离心去除细胞碎片,获得含有可溶蛋白的上清级分用于进一步纯化目的化合物。如果产物从谷氨酸棒状杆菌细胞分泌,则低速离心从培养物中去除细胞,并保留上清级分用于进一步纯化。
将来自任一种纯化方法的上清级分用合适的树脂进行层析,其中目的分子保留在层析树脂上而样本中的许多杂质不保留在层析树脂上,或者杂质保留在层析树脂上而样本不保留。此层析步骤必要时可使用相同或不同的层析树脂重复。本领域普通技术人员在选择合适的层析树脂以及将它们最有效地用于特定的待纯化分子方面是很精通的。可通过过滤或超滤浓缩纯化产物,并贮存在产物稳定性最高的温度下。
本领域公知许多纯化方法,它们不限于上文的纯化方法,并且在如Bailey,J.E.和Ollis,D.F.生物化学工程基础(Biochemical EngineeringFundamentals),McGraw-Hill:纽约(1986)中描述。
分离的化合物的身份和纯度可通过本领域的标准技术确定。这些技术包括高效液相色谱(HPLC)、光谱法、显色法、薄层色谱法、NIRS、酶学试验或微生物学方法。此类分析方法编纂于:Patek等人(1994)Appl.Environ.Microbiol.60:133-140;Malakhova等人(1996)生物技术(Biotekhnologiya)11:27-32;和Schmidt等人(1998)生物加工工程(Bioprocess Engineer)19:67-70;Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)(1996)A27卷,VCH:Weinheim,89-90页,521-540页,540-547页,559-566页,575-581页和581-587页;Michal,G.(1999)生物化学途径:生物化学和分子生物学图集(BiochemicalPathways:An Atlas of Biochemistry and Molecular Biology),John Wileyand Sons;《生物化学和分子生物学中的实验室技术》17卷中的Fallon,A.等人(1987)HPLC在生物化学中的应用。
等价方案
技术人员知晓或者能够通过简单地使用常规的方法确定本发明具体实施方案的大量等价方案。这些等价方案旨在包括在下面的权利要求书中。
表1中信息的理解如下:
在第1列“DNA ID”中,每种情况下相关数字指所附序列表的SEQID NO。所以,“DNA ID”列中的“5”指SEQ ID NO:5。
在第2列“AA ID”中,每种情况下相关数字指所附序列表的SEQ IDNO。因此,“AA ID”列中的“6”指SEQ ID NO:6。
在第3列“标识”中,列出了每个序列的明确的内部名称。
在第4列“AA POS”中,每种情况下相关数字指相同行中多肽序列“AA ID”的氨基酸位置。所以,“AA pos”列中“26”是相应所示的多肽序列的氨基酸位置26。位置计算从N末端以+1开始。
在第5列“AA野生型”中,每种情况下相关字母指相应野生型菌株中位于第4列中所示的位置处的氨基酸(用单字母代码表示)。
在第6列“AA突变体”中,每种情况下相关字母指相应突变菌株中位于第4列中所示的位置处的氨基酸(用单字母代码表示)。
在第7列“功能”中,列出了相应多肽序列的生理学功能。
蛋白原性氨基酸的单字母代码:
A丙氨酸
C半胱氨酸
D天冬氨酸
E谷氨酸
F苯丙氨酸
G甘氨酸
H组氨酸
I异亮氨酸
K赖氨酸
L亮氨酸
M甲硫氨酸
N天冬酰胺
P脯氨酸
Q谷氨酰胺
R精氨酸
S丝氨酸
T苏氨酸
V缬氨酸
W色氨酸
Y酪氨酸
表1
编码新蛋白的基因
DNA AA 标识: AA AA AA 功能:
ID:ID: pos: 野生型 突变体
1 2 RXA00033 158 L F 转运蛋白
382 G E 转运蛋白
3 4 RXA00054 613 A V DNA/RNA解旋酶(DEAD/DEAH Box家族)
5 6 RXA00056 262 G D 假定的输出蛋白
7 8 RXA00065 101 S F 假定的蛋白
9 10 RXA00068 35 S N 假定的蛋白
11 12 RXA00077 374 A T HUNTINGTIN互作蛋白HYPE
13 14 RXA00080 70 G D 整合膜蛋白
253 P S 整合膜蛋白
15 16 RXA00110 172 S F 假定的跨膜蛋白
17 18 RXA00118 53 V I 假定的胞质蛋白
19 20 RXA00121 164 G D 假定的蛋白
21 22 RXA00150 85 G D 假定的跨膜蛋白
23 24 RXA00151 110 V I 假定的蛋白
25 26 RXA00155 231 P S 假定的蛋白
27 28 RXA00185 508 D N 假定的胞质蛋白
29 30 RXA00197 322 A V 膜金属蛋白酶
31 32 RXA00199 12 P S 假定的蛋白
370 L F 假定的蛋白
33 34 RXA00211 44 V I 谷氨酰-tRNA(GLN)氨基转移酶亚基B(EC 6.3.5.-)
35 36 RXA00220 122 G D 乙酰基转移酶(EC 2.3.1.-)
37 38 RXA00240 8 R K 假定的蛋白
39 40 RXA00354 67 G E 水解酶(HAD超家族)
41 42 RXA00397 236 A V XAA-PRO氨肽酶(EC 3.4.11.9)
43 44 RXA00409 217 G D 转运蛋白
45 46 RXA00416 223 A V 假定的跨膜蛋白
47 48 RXA00424 4 R K 假定的蛋白
49 50 RXA00451 78 S N 胞嘧啶脱氨酶(EC 3.5.4.1)
51 52 RXA00476 274 G E 假定的蛋白
287 G R 假定的蛋白
53 54 RXA00486 80 R H 转录调控蛋白,LYSR家族
55 56 RXA00493 363 A T 60KD陪伴蛋白GROEL
57 58 RXA00496 340 P L 假定的蛋白
59 60 RXA00503 186 A T 推断的糖激酶
61 62 RXA00507 224 A V 脂酶(EC 3.1.1.3)
63 64 RXA00510 17 G S 假定的蛋白
65 66 RXA00550 554 S F 青霉素结合蛋白1A
67 68 RXA00552 295 A T 硫代硫酸转硫酶(EC 2.8.1.1)
69 70 RXA00576 157 E K 假定的蛋白
71 72 RXA00611 291 I T 转运蛋白
73 74 RXA00614 86 P S 磷酯酶
75 76 RXA00637 148 E K 转录调控子,MERR家族
77 78 RXA00654 399 S F 内膜蛋白
79 80 RXA00678 127 A T 假定的蛋白
81 82 RXA00691 214 A T 假定的胞质蛋白
83 84 RXA00719 306 D N GTP-结合蛋白
468 A T GTP-结合蛋白
85 86 RXA00731 550 A T 转运蛋白
776 L F 转运蛋白
87 88 RXA00740 93 A T 假定的蛋白
89 90 RXA00812 106 A V 抑制形态分化
91 92 RXA00831 166 A T 假定的胞质蛋白
93 94 RXA00835 54 A V 假定的跨膜蛋白
95 96 RXA00917 635 D N 假定的蛋白
97 98 RXA00943 55 P S 假定的蛋白
123 L F 假定的蛋白
99 100 RXA00963 211 P S 2-羟庚-2,4-二烯-1,7-二酸异构酶(EC 5.3.3.-)
/5-羧甲基-2-氧代-己-3-烯-1,7-二酸脱羧酶(EC 4.1.1.-)
101 102 RXA01011 201 A T 假定的胞质蛋白
103 104 RXA01017 37 V I 假定的胞质蛋白
105 106 RXA01037 28 G D 假定的蛋白
107 108 RXA01046 408 A V ATP-依赖性CLP蛋白酶ATP-结合亚基CLPA
109 110 RXA01109 113 A V 假定的蛋白
111 112 RXA01122 104 R C 假定的蛋白
113 114 RXA01127 60 D N 3-异丙基苹果酸脱氢酶(EC 1.1.1.85)
115 116 RXA01128 221 T I 牛磺酸-结合周质蛋白前体
117 118 RXA01129 2 V I 环核苷酸结合蛋白/2 CBS结构域
119 120 RXA01173 146 A T 假定的分泌蛋白
121 122 RXA01174 174 L F 假定的膜糖蛋白
123 124 RXA01184 363 R K ABC转运整合膜蛋白
430 G D ABC转运整合膜蛋白
125 126 RXA01196 81 G D 假定的胞质蛋白
127 128 RXA01263 74 S F O-抗原乙酰基转移酶
129 130 RXA01268 150 D N CAPM蛋白
131 132 RXA01271 307 S F CAPD蛋白
131 132 RXA01271 525 L F CAPD蛋白
133 134 RXA01273 106 A V 假定的膜结合蛋白
135 136 RXA01315 20 A T 转录调控剂,TETR家族
101 G D 转录调控剂,TETR家族
137 138 RXA01318 67 A V 推断的膜蛋白
139 140 RXA01326 103 A V 假定的蛋白
141 142 RXA01331 13 T I 假定的跨膜蛋白
403 P S 假定的跨膜蛋白
544 T I 假定的跨膜蛋白
143 144 RXA01400 549 G R 假定的胞质蛋白
145 146 RXA01425 85 E K 60KDA内膜蛋白YIDC
147 148 RXA01434 957 S F 毒力因子MVIN
1012 P S 毒力因子MVIN
149 150 RXA01439 116 L F 乙酰基转移酶(EC 2.3.1.-)
151 152 RXA01441 104 C R 假定的蛋白
153 154 RXA01448 159 G D 整合膜蛋白
155 156 RXA01518 148 A T 假定的跨膜蛋白
157 158 RXA01536 121 A V 假定的蛋白
308 A V 假定的蛋白
375 A T 假定的蛋白
159 160 RXA01544 78 V I 假定的蛋白
161 162 RXA01548 153 G D 假定的蛋白
163 164 RXA01549 31 S G 假定的跨膜蛋白
53 A T 假定的跨膜蛋白
159 A T 假定的跨膜蛋白
165 166 RXA01554 124 G D 葡聚糖内切-1,3-β-葡糖苷酶A1前体(EC 3.2.1.39)
486 G R 葡聚糖内切-1,3-β-葡糖苷酶A1前体(EC 3.2.1.39)
167 168 RXA01574 123 P S 糖基转移酶
169 170 RXA01585 112 V M 假定的蛋白Rv2474c
171 172 RXA01590 1432 E K 假定的蛋白
173 174 RXA01628 67 D N 假定的膜糖蛋白
224 G D 假定的膜糖蛋白
175 176 RXA01642 165 G E 胞外枯草杆菌蛋白酶样蛋白酶前体(EC 3.4.21.-)
294 A T 胞外枯草杆菌蛋白酶样蛋白酶前体(EC 3.4.21.-)
177 178 RXA01647 112 D N 假定的膜结合蛋白
167 G S 假定的膜结合蛋白
179 180 RXA01672 56 G D 假定的跨膜蛋白
68 L F 假定的跨膜蛋白
181 182 RXA01677 209 G R 巯基:二硫键互换蛋白DSBA
183 184 RXA01693 102 A T 假定的胞质蛋白
185 186 RXA01741 17 D N 假定的蛋白
187 188 RXA01749 189 A V 具有TRKA结构域的整合膜蛋白
212 P S 具有TRKA结构域的整合膜蛋白
420 A T 具有TRKA结构域的整合膜蛋白
189 190 RXA01754 596 D N 假定的蛋白
191 192 RXA01761 441 R C 假定的蛋白
1376 G S 假定的蛋白
193 194 RXA01770 243 D N DNA解旋酶II(EC 3.6.1.-)
195 196 RXA01771 222 P S 膜蛋白酶亚基,stomatin/Prohibitin同系物
197 198 RXA01776 169 L F 假定的跨膜蛋白
199 200 RXA01778 81 S F 假定的蛋白
201 202 RXA01779 229 T A 假定的输出蛋白
203 204 RXA01789 104 L F 假定的蛋白
205 206 RXA01791 60 A V 假定的蛋白
207 208 RXA01809 128 R Q 假定的膜结合蛋白
209 210 RXA01825 75 G D 假定的跨膜蛋白
211 212 RXA01842 246 P S 醌氧化还原酶(EC 1.6.5.5)
213 214 RXA01899 233 S N 假定的胞质蛋白
215 216 RXA01905 103 V I 假定的输出蛋白
219 220 RXA01910 220 G S 假定的蛋白
221 222 RXA01945 629 E K 假定的输出蛋白
1382 G D 假定的输出蛋白
223 224 RXA01966 46 A V 寡聚核糖核酸酶(EC 3.1.-.-)
225 226 RXA01982 341 A T 假定的蛋白
227 228 RXA02021 305 S F 2,3,4,5-四氢吡啶-2-羧酸N-琥珀酰转移酶(同系物)
229 230 RXA02032 208 G S 整合膜蛋白
231 232 RXA02053 66 H Y DRGA蛋白
233 234 RXA02058 188 S F 假定的蛋白
235 236 RXA02059 24 G R 假定的跨膜蛋白
237 238 RXA02070 150 A T MRP蛋白同系物
239 240 RXA02084 105 A V 假定的蛋白
241 242 RXA02091 170 E K 假定的蛋白
243 244 RXA02097 28 A V 转录调控剂
427 G R 转录调控剂
245 246 RXA02104 39 T I 甘油酸激酶(EC 2.7.1.31)
247 248 RXA02123 678 L F 镁-螯合酶亚基CHLI
249 250 RXA02138 88 A V HESB蛋白
251 252 RXA02151 165 A T 假定的跨膜蛋白
253 254 RXA02163 23 P S 假定的蛋白
255 256 RXA02185 104 G S RPF蛋白前体
257 258 RXA02186 39 S F 假定的胞质蛋白
259 260 RXA02203 36 D N 假定的蛋白
261 262 RXA02206 73 G D 氧化还原酶(EC 1.1.1.-)
114 A T 氧化还原酶(EC 1.1.1.-)
314 R C 氧化还原酶(EC 1.1.1.-)
263 264 RXA02217 65 E K 金鸡钠酸脱氢酶(吡咯并喹啉-苯醌)(EC 1.1.99.25)
92 T I 金鸡钠酸脱氢酶(吡咯并喹啉-苯醌)(EC 1.1.99.25)
265 266 RXA02219 292 V M 假定的胞质蛋白
267 268 RXA02221 176 L F 假定的蛋白
269 270 RXA02267 65 A T DNA(胞嘧啶-5)-甲基转移酶(EC 2.1.1.37)
271 272 RXA02271 126 G D 假定的输出蛋白
273 274 RXA02280 502 A V 热休克蛋白HTPG
275 276 RXA02294 57 E K 假定的蛋白
277 278 RXA02295 130 G D 转运蛋白
279 280 RXA02296 61 S N 假定的输出蛋白
281 282 RXA02298 447 L F 假定的胞质蛋白
283 284 RXA02302 279 G E 氯霉素-敏感蛋白RARD
285 286 RXA02303 268 G D 假定的蛋白
289 290 RXA02339 38 P S 假定的胞质蛋白
291 292 RXA02360 243 V I I类限制-修饰系统DNA甲基化酶
293 294 RXA02362 525 M I 转录调控剂
295 296 RXA02367 3 I V PIRIN
297 298 RXA02387 10 A T 假定的胞质蛋白
299 300 RXA02390 195 G D 苏氨酸流出蛋白
301 302 RXA02395 533 A V 转运蛋白
303 304 RXA02425 34 G E 重金属阳离子转运蛋白
305 306 RXA02433 9 S N 假定的蛋白
307 308 RXA02486 141 T I 假定的胞质蛋白
309 310 RXA02488 27 A T 血红素转运结合蛋白(heme transport associated Protein)
311 312 RXA02495 81 G S ABC转运整合膜蛋白
313 314 RXA02570 184 R C ABC转运整合膜蛋白
315 316 RXA02591 377 P S 磷酸烯醇丙酮酸羧激酶[GTP](EC 4.1.1.32)
317 318 RXA02604 570 G D 假定的跨膜蛋白
319 320 RXA02678 3 M I 假定的胞质蛋白
321 322 RXA02683 81 G S 假定的蛋白
323 324 RXA02721 181 G S 假定的胞质蛋白
325 326 RXA02725 208 A V 假定的蛋白
327 328 RXA02735 27 P S 6-磷酸葡萄糖内酯酶(EC 3.1.1.31)
329 330 RXA02736 312 S F 推断的OXPPCYCLE蛋白OPCA
331 332 RXA02744 231 M I 推断的整合膜转运蛋白
333 334 RXA02757 73 P S 假定的蛋白
335 336 RXA02777 306 G D 假定的胞质蛋白
337 338 RXA02784 4 A V 假定的蛋白
339 340 RXA02786 292 E K 假定的蛋白
341 342 RXA02798 457 L S 亚硫酸盐还原酶(NADPH)黄素蛋白α-组分(EC 1.8.1.2)
343 344 RXA02817 227 G D AEFA蛋白
345 346 RXA02825 362 S F 血红素转运结合蛋白
347 348 RXA02951 26 L F 假定的胞质蛋白
349 350 RXA03010 106 G S 假定的蛋白
351 352 RXA03029 6 K E 转录调控剂
7 P A 转录调控剂
8 C I 转录调控剂
353 354 RXA03053 99 M I 假定的蛋白
355 356 RXA03098 164 S N DNA烷基化修复酶
357 358 RXA03113 374 P L 具有TRKA结构域的整合膜蛋白
359 360 RXA03184 109 S F 转运蛋白
361 362 RXA03316 6 D N
363 364 RXA03343 171 S F
365 366 RXA03377 55 A V
367 368 RXA03463 53 V I
369 370 RXA03506 80 P L 假定的胞质蛋白
371 372 RXA03523 167 A T
373 374 RXA03572 2 P L
375 376 RXA03577 72 A T
377 378 RXA03584 12 S F
379 380 RXA03591 902 S F
381 382 RXA03613 106 S Y
383 384 RXA03682 18 P S
385 386 RXA03700 139 E K
269 A T
387 388 RXA03803 44 P S
389 390 RXA03823 20 V I
391 392 RXA03886 23 A V
393 394 RXA03940 86 T I
395 396 RXA03947 81 A T
84 S N
397 398 RXA03955 126 T I
399 400 RXA03975 55 V I
401 402 RXA04007 145 S N
403 404 RXA04075 66 G S
173 A V
405 406 RXA04085 55 P S
407 408 RXA04092 18 A V
409 410 RXA04095 30 R K
411 412 RXA04101 40 T I
413 414 RXA04169 21 T I
415 416 RXA04172 46 P S
417 418 RXA04181 40 G D
419 420 RXA04319 40 R C
421 422 RXA04337 19 P S
423 424 RXA04352 2 G S
425 426 RXA04354 50 T I
427 428 RXA04370 102 P L
429 430 RXA04546 85 A V 假定的胞质蛋白
362 P L 假定的胞质蛋白
431 432 RXA04549 214 G R
435 436 RXA04642 112 P L
437 438 RXA06004 148 D N
439 440 RXA06040 58 S P
59 I M
93 V E
序列表
序列表
<110>巴斯福股份公司(BASF Aktiengesellschaft)
<120>编码新蛋白的基因
<130>O.Z.0050/52976
<160>434
<210>1
<211>1465
<212>DNA
<213>谷氨酸棒状杆菌(Corynebacterium glutamicum)
<220>
<221>CDS
<222>(101)..(1435)
<223>RXA00033
<400>1
acacggattt aacgtgacgg tttctcagtg tttttagctt ctggcttttg gcttctgatg 60
ctttagcgct ggactcaaca tttaaaacaa aggtgaacac atg tca gta cag caa 115
Met Ser Val Gln Gln
1 5
agc ggg ttg ctc gaa cgt ctc ggc att ccc cga ccc ttg att ttc gga 163
Ser Gly Leu Leu Glu Arg Leu Gly Ile Pro Arg Pro Leu Ile Phe Gly
10 15 20
ttt atc ggc ctc acc atc ttc atg atc ggt gac ggt gtc gaa acc aac 211
Phe Ile Gly Leu Thr Ile Phe Met Ile Gly Asp Gly Val Glu Thr Asn
25 30 35
att ttg gaa cct ttc ctc agt tcc gaa cat ggt ttc agc gtt tcc ctc 259
Ile Leu Glu Pro Phe Leu Ser Ser Glu His GlV Phe Ser Val Ser Leu
40 45 50
gcg gga acc ctg gtg act gtt tac ggt gtt gcc gtg gcg atc gca gca 307
Ala Gly Thr Leu Val Thr Val Tyr Gly Val Ala Val Ala Ile Ala Ala
55 60 65
ttc ttc gcg gcg gca ctt tcg gac ctg tgg ggt cca cga aaa gtg atg 355
Phe Phe Ala Ala Ala Leu Ser Asp Leu Trp Gly Pro Arg Lys Val Met
70 75 80 85
atc ctc ggt gct tca atc tgg atc gtc ttt gag ctg atc ttc ctc acc 403
Ile Leu Gly Ala Ser Ile Trp Ile Val Phe Glu Leu Ile Phe Leu Thr
90 95 100
gtg gca ctg acc acc gac cat act tgg ttg atc ttc ctt gct tat ggt 451
Val Ala Leu Thr Thr Asp His Thr Trp Leu Ile Phe Leu Ala Tyr Gly
105 110 115
ctc cgc ggc ttt ggt tat cca ttc ttc gcc tac gga ttc ttg gtg tgg 499
Leu Arg Gly Phe Gly Tyr pro Phe Phe Ala Tyr Gly Phe Leu Val Trp
120 125 130
atc acc gca act gcc tca cct aag caa ttg ggt acc ggt gtg ggt tgg 547
Ile Thr Ala Thr Ala Ser Pro Lys Gln Leu Gly Thr Gly Val Gly Trp
135 140 145
ttc tac gtt gcc ttc tct gca ggt ctt cct acc ttg ggt gcg ctg gtt 595
Phe Tyr Val Ala Phe Ser Ala Gly Leu Pro Thr Leu Gly Ala Leu Val
150 155 160 165
gcc acc att tcc atg cag tac gtg aac ttg acc ttc tat gaa acg ttg 643
Ala Thr Ile Ser Met Gln Tyr Val Asn Leu Thr Phe Tyr Glu Thr Leu
170 175 180
tgg gtt tcc ctc gtg ctg gtg gtc atc gga tcg ctc atc gca ctg ctg 691
Trp Val Ser Leu Val Leu Val Val Ile Gly Ser Leu Ile Ala Leu Leu
185 190 195
gga gtg aag gaa cgt cgc gga cgc cac cca ctg gtt gcc aac ccc gac 739
Gly Val Lys Glu Arg Arg Gly Arg His Pro Leu Val Ala Asn Pro Asp
200 205 210
gat gtg aag caa aca ctt ggc cag ggc ttc aaa ctt ctg cgc aat gat 787
Asp Val Lys Gln Thr Leu Gly Gln Gly Phe Lys Leu Leu Arg Asn Asp
215 220 225
cga cgt gca cgt ttt gtc acc tac atc cgc acc atc aac tcc att ccg 835
Arg Arg Ala Arg Phe Val Thr Tyr Ile Arg Thr Ile Asn Ser Ile Pro
230 235 240 245
acc tac gcg atg gct gtg ttc ttc cca tca ttt ttc act gac gat ctg 883
Thr Tyr Ala Met Ala Val Phe Phe Pro Ser Phe Phe Thr Asp Asp Leu
250 255 260
aag tgg cag cta agc tgg ttc ctc atc ctc acc act gta att tac gca 931
Lys Trp Gln Leu Ser Trp Phe Leu Ile Leu Thr Thr Val Ile Tyr Ala
265 270 275
gtc aac ctg ccg ttc aat cct ttc ttc ggt agc ttc ggc gac cgc cac 979
Val Asn Leu Pro Phe Asn Pro Phe Phe Gly Ser Phe Gly Asp Arg His
280 285 290
ggt tgg gca cga act gtg ttc tgg ggc gga tca atc ggt ggc gca gtc 1027
Gly Trp Ala Arg Thr Val Phe Trp Gly Gly Ser Ile Gly Gly Ala Val
295 300 305
acc ctc gcg ttg gtt tac ttc att ccg atg ttc ggc gtt cag gct ggc 1075
Thr Leu Ala Leu Val Tyr Phe Ile Pro Met Phe Gly Val Gln Ala Gly
310 315 320 325
atg tcc aac ggt gtc gtt ttc gga atc acc atc gca gcc ggc gca ctc 1123
Met Ser Asn Gly Val Val Phe Gly Ile Thr Ile Ala Ala Gly Ala Leu
330 335 340
ttt ggt gtg tcc ctc gcg ggc ttc gtg cca ctt tcc gca atc gct gtc 1171
Phe Gly Val Ser Leu Ala Gly Phe Val Pro Leu Ser Ala Ile Ala Val
345 350 355
tcc ctt gat ccc aag cac ccc ggc gca gcg atg gcc aca tac aac ctc 1219
Ser Leu Asp Pro Lys His Pro Gly Ala Ala Met Ala Thr Tyr Asn Leu
360 365 370
ggc gtt ggt ggc gct gta gct gtg gga ccg ctc ctg gtt gca gtc ttc 1267
Gly Val Gly Gly Ala Val Ala Val Gly Pro Leu Leu Val Ala Val Phe
375 380 385
cac cca ctg att ggt cca acc gga ttg atc ctg gtc atg atc gcc ctc 1315
His Pro Leu Ile Gly Pro Thr Gly Leu Ile Leu Val Met Ile Ala Leu
390 395 400 405
tac ctg ctc tcc ggt tgg atg act ctt caa ctt cgc ggc acc caa cca 1363
Tyr Leu Leu Ser Gly Trp Met Thr Leu Gln Leu Arg Gly Thr Gln Pro
410 415 420
gga ttc gac gga gtg cca gca ctt gct gaa gac gcc cac atc gaa gac 1411
Gly Phe Asp Gly Val Pro Ala Leu Ala Glu Asp Ala His Ile Glu Asp
425 430 435
ctt gca gat gta aac gca aac gcc taactgtttt tcgagctaaa cccatccttg 1465
Leu Ala Asp Val Asn Ala Asn Ala
440 445
<210>2
<211>445
<212>PRT
<213>谷氨酸棒状杆菌
<400>2
Met Ser Val Gln Gln Ser Gly Leu Leu Glu Arg Leu Gly Ile Pro Arg
1 5 10 15
Pro Leu Ile Phe Gly Phe Ile Gly Leu Thr Ile Phe Met Ile Gly Asp
20 25 30
Gly Val Glu Thr Asn Ile Leu Glu Pro Phe Leu Ser Ser Glu His Gly
35 40 45
Phe Ser Val Ser Leu Ala Gly Thr Leu Val Thr Val Tyr Gly Val Ala
50 55 60
Val Ala Ile Ala Ala Phe Phe Ala Ala Ala Leu Ser Asp Leu Trp Gly
65 70 75 80
Pro Arg Lys Val Met Ile Leu Gly Ala Ser Ile Trp Ile Val Phe Glu
85 90 95
Leu Ile Phe Leu Thr Val Ala Leu Thr Thr Asp His Thr Trp Leu Ile
100 105 110
Phe Leu Ala Tyr Gly Leu Arg Gly Phe Gly Tyr Pro Phe Phe Ala Tyr
115 120 125
Gly Phe Leu Val Trp Ile Thr Ala Thr Ala Ser Pro Lys Gln Leu Gly
130 135 140
Thr Gly Val Gly Trp Phe Tyr Val Ala Phe Ser Ala Gly Leu Pro Thr
145 150 155 160
Leu Gly Ala Leu Val Ala Thr Ile Ser Met Gln Tyr Val Asn Leu Thr
165 170 175
Phe Tyr Glu Thr Leu Trp Val Ser Leu Val Leu Val Val Ile Gly Ser
180 185 190
Leu Ile Ala Leu Leu Gly Val Lys Glu Arg Arg Gly Arg His Pro Leu
195 200 205
Val Ala Asn Pro Asp Asp Val Lys Gln Thr Leu Gly Gln Gly Phe Lys
210 215 220
Leu Leu Arg Asn Asp Arg Arg Ala Arg Phe Val Thr Tyr Ile Arg Thr
225 230 235 240
Ile Asn Ser Ile Pro Thr Tyr Ala Met Ala Val Phe Phe Pro Ser Phe
245 250 255
Phe Thr Asp Asp Leu Lys Trp Gln Leu Ser Trp Phe Leu Ile Leu Thr
260 265 270
Thr Val Ile Tyr Ala Val Asn Leu Pro Phe Asn Pro Phe Phe Gly Ser
275 280 285
Phe Gly Asp Arg His Gly Trp Ala Arg Thr Val Phe Trp Gly Gly Ser
290 295 300
Ile Gly Gly Ala Val Thr Leu Ala Leu Val Tyr Phe Ile Pro Met Phe
305 310 315 320
Gly Val Gln Ala Gly Met Ser Asn Gly Val Val Phe Gly Ile Thr Ile
325 330 335
Ala Ala Gly Ala Leu Phe Gly Val Ser Leu Ala Gly Phe Val Pro Leu
340 345 350
Ser Ala Ile Ala Val Ser Leu Asp Pro Lys His Pro Gly Ala Ala Met
355 360 365
Ala Thr Tyr Asn Leu Gly Val Gly Gly Ala Val Ala Val Gly Pro Leu
370 375 380
Leu Val Ala Val Phe His Pro Leu Ile Gly Pro Thr Gly Leu Ile Leu
385 390 395 400
Val Met Ile Ala Leu Tyr Leu Leu Ser Gly Trp Met Thr Leu Gln Leu
405 410 415
Arg Gly Thr Gln Pro Gly Phe Asp Gly Val Pro Ala Leu Ala Glu Asp
420 425 430
Ala His Ile Glu Asp Leu Ala Asp Val Asn Ala Asn Ala
435 440 445
<210>3
<211>3043
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3013)
<223>RXA00054
<400>3
atgctgagat ccgctgggtg gcaccacacg aattggaatc tttggagtgg gcacctgctg 60
atattcctgc ggtgaaactt ctcgtcgagc agcttgctta atg agc cca ttc gat 115
Met Ser Pro Phe Asp
1 5
tca aag ctt ggt cgc gat acc cat ttt gga ttc ctc gat aag acc act 163
Ser Lys Leu Gly Arg Asp Thr His Phe Gly Phe Leu Asp Lys Thr Thr
10 15 20
gct tcg caa caa ctg ctg aat cct tca ttg att tct aat gag gat ccg 211
Ala Ser Gln Gln Leu Leu Asn Pro Ser Leu Ile Ser Asn Glu Asp Pro
25 30 35
cac acc atg ttg cag gcg atc aag tta gag ctt cgt acc gca cag tct 259
His Thr Met Leu Gln Ala Ile Lys Leu Glu Leu Arg Thr Ala Gln Ser
40 45 50
ttt aca ttt tca gtc gct ttt att tcc agt cgc ggt atc gcg ttg ttg 307
Phe Thr Phe Ser Val Ala Phe Ile Ser Ser Arg Gly Ile Ala Leu Leu
55 60 65
aaa cag gca ttg ttg gac ttc aag ggt aaa gga cga att atc acg tcc 355
Lys Gln Ala Leu Leu Asp Phe Lys Gly Lys Gly Arg Ile Ile Thr Ser
70 75 80 85
cgc tac ctg gat ttc aat gat cca acg atg ttt cgt gag ctt ctt act 403
Arg Tyr Leu Asp Phe Asn Asp Pro Thr Met Phe Arg Glu Leu Leu Thr
90 95 100
ttg gag aac gtg gaa gtc ctc ata cat caa ggc gat ggt ttc cac tcc 451
Leu Glu Asn Val Glu Val Leu Ile His Gln Gly Asp Gly Phe His Ser
105 110 115
aag ggc tac gtt ttc cat cac gac gtg gga atc acc gca gta gtg ggt 499
Lys Gly Tyr Val Phe His His Asp Val Gly Ile Thr Ala Val Val Gly
120 125 130
agt tcc aac ctc aca gat aat gct ctg ttg gta aac cgg gaa tgg aat 547
Ser Ser Asn Leu Thr Asp Asn Ala Leu Leu Val Asn Arg Glu Trp Asn
135 140 145
ctg aag ttc tct gcc agc aag aat ggc gac att gct ttt caa ctt gat 595
Leu Lys Phe Ser Ala Ser Lys Asn Gly Asp Ile Ala Phe Gln Leu Asp
150 155 160 165
gat gcg att aat cgc caa att gaa cga tcc acg cca ctg acg cct gaa 643
Asp Ala Ile Asn Arg Gln Ile Glu Arg Ser Thr Pro Leu Thr Pro Glu
170 175 180
tgg atc tca gag tat gag gca acc aga cgt gta cct gag cgt ttg gtg 691
Trp Ile Ser Glu Tyr Glu Ala Thr Arg Arg Val Pro Glu Arg Leu Val
185 190 195
tct cag aat att cct ctc gag gat caa agt aac gcc gga aca att gtt 739
Ser Gln Asn Ile Pro Leu Glu Asp Gln Ser Asn Ala Gly Thr Ile Val
200 205 210
cca aac gtc atg cag gaa gag gcc ctc gac gct cta ctt tct ttg acg 787
Pro Asn Val Met Gln Glu Glu Ala Leu Asp Ala Leu Leu Ser Leu Thr
215 220 225
gag aag gga gag aaa cgt ggt gtc att att tct gcg aca gga act ggc 835
Glu Lys Gly Glu Lys Arg Gly Val Ile Ile Ser Ala Thr Gly Thr Gly
230 235 240 245
aag act atc ctg gct gct cta gct aca aga atg ctc aag cct gaa cga 883
Lys Thr Ile Leu Ala Ala Leu Ala Thr Arg Met Leu Lys Pro Glu Arg
250 255 260
gtt ttg ttt gtc gtg cac cgc gaa caa att ctg gat aag gcg cga tcc 931
Val Leu Phe Val Val His Arg Glu Gln Ile Leu Asp Lys Ala Arg Ser
265 270 275
gag ttc atc aaa gtt ctt gaa cgt ccc gct gtt gat ttc gga aaa atg 979
Glu Phe Ile Lys Val Leu Glu Arg Pro Ala Val Asp Phe Gly Lys Met
280 285 290
tct ggc tcg acc aag gag cta gat aag ccg ttt gtg ttt ggc acc atc 1027
Ser Gly Ser Thr Lys Glu Leu Asp Lys Pro Phe Val Phe Gly Thr Ile
295 300 305
caa acg ctg aca aag gaa gct aca ctt tcc cag atc tcc cct act gac 1075
Gln Thr Leu Thr Lys Glu Ala Thr Leu Ser Gln Ile Ser Pro Thr Asp
310 315 320 325
ttt gat tta gtc atc gtc gat gaa gtc cat cgc gcc ggt gcc gaa tcc 1123
Phe Asp Leu Val Ile Val Asp Glu Val His Arg Ala Gly Ala Glu Ser
330 335 340
tat ttg gca ttg otc aat cat ctg cag cca cag ttt tta ttg ggc ctg 1171
Tyr Leu Ala Leu Leu Asn His Leu Gln Pro Gln Phe Leu Leu Gly Leu
345 350 355
act gct acc cca gag cga act gat gga ttc aat att tat gag ttg ttc 1219
Thr Ala Thr Pro Glu Arg Thr Asp Gly Phe Asn Ile Tyr Glu Leu Phe
360 365 370
gat ttc aac gtg cca tat gag atc cga ctt caa gct gct tta gaa tcc 1267
Asp Phe Asn Val Pro Tyr Glu Ile Arg Leu Gln Ala Ala Leu Glu Ser
375 380 385
aac atg ctc gta ccg ttt cac tat tac ggt gtc aca gac ttc acg ctt 1315
Asn Met Leu Val Pro Phe His Tyr Tyr Gly Val Thr Asp Phe Thr Leu
390 395 400 405
gat tct gaa aca acg gtg act gat acc tcc aaa cta agt gcc ttg gtg 1363
Asp Ser Glu Thr Thr Val Thr Asp Thr Ser Lys Leu Ser Ala Leu Val
410 415 420
agc gaa gag aga gtc cat cac att ctg gag gcc ctc aaa act tat ggt 1411
Ser Glu Glu Arg Val His His Ile Leu Glu Ala Leu Lys Thr Tyr Gly
425 430 435
cat cca gaa aat gtt cgt gga ctg atc ttc tgt tcc aag act gaa gag 1459
His Pro Glu Asn Val Arg Gly Leu Ile Phe Cys Ser Lys Thr Glu Glu
440 445 450
gct gag gag ctg tca aaa ctt ctc gac cag tcg ttg ttt aat ggc agc 1507
Ala Glu Glu Leu Ser Lys Leu Leu Asp Gln Ser Leu Phe Asn Gly Ser
455 460 465
ttg ctt aaa acc aag gcg ctc tct gcg aag gac tca att ccc tac cgc 1555
Leu Leu Lys Thr Lys Ala Leu Ser Ala Lys Asp Ser Ile Pro Tyr Arg
470 475 480 485
gaa gag gtt gta gct gag ctg gag tcc ggc gac ctg gac tac atc ttg 1603
Glu Glu Val Val Ala Glu Leu Glu Ser Gly Asp Leu Asp Tyr Ile Leu
490 495 500
acg gtt gac atc ttc aat gaa ggc att gat att cct tcg gtg aat caa 1651
Thr Val Asp Ile Phe Asn Glu Gly Ile Asp Ile Pro Ser Val Asn Gln
505 510 515
atc gta atg att cgt agt act caa tca agc att gtg ttt acg cag cag 1699
Ile Val Met Ile Arg Ser Thr Gln Ser Ser Ile Val Phe Thr Gln Gln
520 525 530
ctc ggg cgt gga ctc cga aaa gct gct ggc aaa gac cat ctg cgc gtt 1747
Leu Gly Arg Gly Leu Arg Lys Ala Ala Gly Lys Asp His Leu Arg Val
535 540 545
att gat ttc att ggc aac tac gcg aac aac tat ctc att ccc atc gca 1795
Ile Asp Phe Ile Gly Asn Tyr Ala Asn Asn Tyr Leu Ile Pro Ile Ala
550 555 560 565
ttg ttt ggc gat aat tct cgc aat aag aac agc atc aga cgc cgc ctg 1843
Leu Phe Gly Asp Asn Ser Arg Asn Lys Asn Ser Ile Arg Arg Arg Leu
570 575 580
att gag agc gat att gat gga aca atc tca ggt gtt tca agt gtt aac 1891
Ile Glu Ser Asp Ile Asp Gly Thr Ile Ser Gly Val Ser Set Val Asn
585 590 595
ttt gat ccc atc gcg cag gag aga att ttt gct gcg ttg aag gct gcg 1939
Phe Asp Pro Ile Ala Gln Glu Arg Ile Phe Ala Ala Leu Lys Ala Ala
600 605 610
aag ttg gac tca aaa gca caa ttc aag cag gat att gtt cag ctt caa 1987
Lys Leu Asp Ser Lys Ala Gln Phe Lys Gln Asp Ile Val Gln Leu Gln
615 620 625
gat cga ctc aat cat gtg cca gca ctg tta gac ttc gct cgc ttc aat 2035
Asp Arg Leu Asn His Val Pro Ala Leu Leu Asp Phe Ala Arg Phe Asn
630 635 640 645
act gtt gat ccg ttt atc ctt gcc acg cat tct ggc aac tac tgg tcg 2083
Thr Val Asp Pro Phe Ile Leu Ala Thr His Ser Gly Asn Tyr Trp Ser
650 655 660
ctg ctc agt tca gtg aag ttt gtc gat cac gct ccc agc gaa tcg gag 2131
Leu Leu Ser Ser Val Lys Phe Val Asp His Ala Pro Ser Glu Ser Glu
665 670 675
aag tac ttt ctg gat ttc ctt acc gga gag ctt ctt aac ggc aag cga 2179
Lys Tyr Phe Leu Asp Phe Leu Thr Gly Glu Leu Leu Asn Gly Lys Arg
680 685 690
cct cat gag ctg ttg ctg atc cag gaa cta atg aaa cag cct gaa act 2227
Pro His Glu Leu Leu Leu Ile Gln Glu Leu Met Lys Gln Pro Glu Thr
695 700 705
tct act gaa gaa ttc cgt aag ctc ttg cag gcc caa tcg act agt tca 2275
Ser Thr Glu Glu Phe Arg Lys Leu Leu Gln Ala Gln Ser Thr Ser Ser
710 715 720 725
gat gag caa aca atc aac tcg gtt gaa agg att ttg agc caa gaa ttc 2323
Asp Glu Gln Thr Ile Asn Ser Val Glu Arg Ile Leu Ser Gln Glu Phe
730 735 740
tat acg gga cca aac cgc aag aag ttt ggc gaa cat ccg atc ctc tct 2371
Tyr Thr Gly Pro Asn Arg Lys Lys Phe Gly Glu His Pro Ile Leu Ser
745 750 755
gtt caa aac cgc acc tac tct ttt act ccc gag ttc cga cgt gcc ctc 2419
Val Gln Asn Arg Thr Tyr Ser Phe Thr Pro Glu Phe Arg Arg Ala Leu
760 765 770
gac gaa agc cta gaa gtg acg gac cga gat gag gct gct cag aat ttt 2467
Asp Glu Ser Leu Glu Val Thr Asp Arg Asp Glu Ala Ala Gln Asn Phe
775 780 785
aag ttt cac gtc caa gac atc atc gac act ggt ttg ttt atc gcc aga 2515
Lys Phe His Val Gln Asp Ile Ile Asp Thr Gly Leu Phe Ile Ala Arg
790 795 800 805
aac aat gga ttt tgg caa gga aac ctc gtt gtt ggc gag aga tac tcc 2563
Asn Asn Gly Phe Trp Gln Gly Asn Leu Val Val Gly Glu Arg Tyr Ser
810 815 820
cga cga gat gtc tgc cga att ctc aat tgg gaa cga aac aac gag agc 2611
Arg Arg Asp Val Cys Arg Ile Leu Asn Trp Glu Arg Asn Asn Glu Ser
825 830 835
acg att tat gga tac aaa gtg gac agt tac aca tct act tgc cca atc 2659
Thr Ile Tyr Gly Tyr Lys Val Asp Ser Tyr Thr Ser Thr Cys Pro Ile
840 845 850
ttt gtg acc tat cac aag gct gat gat gta tcc gaa agt act cgt tac 2707
Phe Val Thr Tyr His Lys Ala Asp Asp Val Ser Glu Ser Thr Arg Tyr
855 860 865
cag gat gaa ctc gtc gat ccg aat acc ctt cat tgg tat tcc cgc ggc 2755
Gln Asp Glu Leu Val Asp Pro Asn Thr Leu His Trp Tyr Ser Arg Gly
870 875 880 885
aac cga aag atc acg tct aat gag atc aag ccc atc gct gcg aat gct 2803
Asn Arg Lys Ile Thr Ser Asn Glu Ile Lys Pro Ile Ala Ala Asn Ala
890 895 900
gtg gat ctt cat gtt ttt gtg aag aag gac gat gcc gaa ggc ctt gat 2851
Val Asp Leu His Val Phe Val Lys Lys Asp Asp Ala Glu Gly Leu Asp
905 910 915
ttc ttc tac ctt ggt caa gcg cat tca gaa aac agc aaa cag tca tcg 2899
Phe Phe Tyr Leu Gly Gln Ala His Ser Glu Asn Ser Lys Gln Ser Ser
920 925 930
atg ccc gga aac aaa gga gtt gtg caa ccg gtg gtc aca atg gat cta 2947
Met Pro Gly Asn Lys Gly Val Val Gln Pro Val Val Thr Met Asp Leu
935 940 945
cag ttc gac aca ccc gtc gaa caa agc ctg ttt gag tac ctg agc aca 2995
Gln Phe Asp Thr Pro Val Glu Gln Ser Leu Phe Glu Tyr Leu Ser Thr
950 955 960 965
aat ctc gcc gta acg gag taaccaccgc aaccaagcgt cgaaaagcaa 3043
Asn Leu Ala Val Thr Glu
970
<210>4
<211>971
<212>PRT
<213>谷氨酸棒状杆菌
<400>4
Met Ser Pro Phe Asp Ser Lys Leu Gly Arg Asp Thr His Phe Gly Phe
1 5 10 15
Leu Asp Lys Thr Thr Ala Ser Gln Gln Leu Leu Asn Pro Ser Leu Ile
20 25 30
Ser Asn Glu Asp Pro His Thr Met Leu Gln Ala Ile Lys Leu Glu Leu
35 40 45
Arg Thr Ala Gln Ser Phe Thr Phe Ser Val Ala Phe Ile Ser Ser Arg
50 55 60
Gly Ile Ala Leu Leu Lys Gln Ala Leu Leu Asp Phe Lys Gly Lys Gly
65 70 75 80
Arg Ile Ile Thr Ser Arg Tyr Leu Asp Phe Asn Asp Pro Thr Met Phe
85 90 95
Arg Glu Leu Leu Thr Leu Glu Asn Val Glu Val Leu Ile His Gln Gly
100 105 110
Asp Gly Phe His Ser Lys Gly Tyr Val Phe His His Asp Val Gly Ile
115 120 125
Thr Ala Val Val Gly Ser Ser Asn Leu Thr Asp Asn Ala Leu Leu Val
130 135 140
Asn Arg Glu Trp Asn Leu Lys Phe Ser Ala Ser Lys Asn Gly Asp Ile
145 150 155 160
Ala Phe Gln Leu Asp Asp Ala Ile Asn Arg Gln Ile Glu Arg Ser Thr
165 170 175
Pro Leu Thr Pro Glu Trp Ile Ser Glu Tyr Glu Ala Thr Arg Arg Val
180 185 190
Pro Glu Arg Leu Val Ser Gln Asn Ile Pro Leu Glu Asp Gln Ser Asn
195 200 205
Ala Gly Thr Ile Val Pro Asn Val Met Gln Glu Glu Ala Leu Asp Ala
210 215 220
Leu Leu Ser Leu Thr Glu Lys Gly Glu Lys Arg Gly Val Ile Ile Ser
225 230 235 240
Ala Thr Gly Thr Gly Lys Thr Ile Leu Ala Ala Leu Ala Thr Arg Met
245 250 255
Leu Lys Pro Glu Arg Val Leu Phe Val Val His Arg Glu Gln Ile Leu
260 265 270
Asp Lys Ala Arg Ser Glu Phe Ile Lys Val Leu Glu Arg Pro Ala Val
275 280 285
Asp Phe Gly Lys Met Ser Gly Ser Thr Lys Glu Leu Asp Lys Pro Phe
290 295 300
Val Phe Gly Thr Ile Gln Thr Leu Thr Lys Glu Ala Thr Leu Ser Gln
305 310 315 320
Ile Ser Pro Thr Asp Phe Asp Leu Val Ile Val Asp Glu Val His Arg
325 330 335
Ala Gly Ala Glu Ser Tyr Leu Ala Leu Leu Asn His Leu Gln Pro Gln
340 345 350
Phe Leu Leu Gly Leu Thr Ala Thr Pro Glu Arg Thr Asp Gly Phe Asn
355 360 365
Ile Tyr Glu Leu Phe Asp Phe Asn Val Pro Tyr Glu Ile Arg Leu Gln
370 375 380
Ala Ala Leu Glu Ser Asn Met Leu Val Pro Phe His Tyr Tyr Gly Val
385 390 395 400
Thr Asp Phe Thr Leu Asp Ser Glu Thr Thr Val Thr Asp Thr Ser Lys
405 410 415
Leu Ser Ala Leu Val Ser Glu Glu Arg Val His His Ile Leu Glu Ala
420 425 430
Leu Lys Thr Tyr Gly His Pro Glu Asn Val Arg Gly Leu Ile Phe Cys
435 440 445
Ser Lys Thr Glu Glu Ala Glu Glu Leu Ser Lys Leu Leu Asp Gln Ser
450 455 460
Leu Phe Asn Gly Ser Leu Leu Lys Thr Lys Ala Leu Ser Ala Lys Asp
465 470 475 480
Ser Ile Pro Tyr Arg Glu Glu Val Val Ala Glu Leu Glu Ser Gly Asp
485 490 495
Leu Asp Tyr Ile Leu Thr Val Asp Ile Phe Asn Glu Gly Ile Asp Ile
500 505 510
Pro Ser Val Asn Gln Ile Val Met Ile Arg Ser Thr Gln Ser Ser Ile
515 520 525
Val Phe Thr Gln Gln Leu Gly Arg Gly Leu Arg Lys Ala Ala Gly Lys
530 535 540
Asp His Leu Arg Val Ile Asp Phe Ile Gly Asn Tyr Ala Asn Asn Tyr
545 550 555 560
Leu Ile Pro Ile Ala Leu Phe Gly Asp Asn Ser Arg Asn Lys Asn Ser
565 570 575
Ile Arg Arg Arg Leu Ile Glu Ser Asp Ile Asp Gly Thr Ile Ser Gly
580 585 590
Val Ser Ser Val Asn Phe Asp Pro Ile Ala Gln Glu Arg Ile Phe Ala
595 600 605
Ala Leu Lys Ala Ala Lys Leu Asp Ser Lys Ala Gln Phe Lys Gln Asp
610 615 620
Ile Val Gln Leu Gln Asp Arg Leu Asn His Val Pro Ala Leu Leu Asp
625 630 635 640
Phe Ala Arg Phe Asn Thr Val Asp Pro Phe Ile Leu Ala Thr His Ser
645 650 655
Gly Asn Tyr Trp Ser Leu Leu Ser Ser Val Lys Phe Val Asp His Ala
660 665 670
Pro Ser Glu Ser Glu Lys Tyr Phe Leu Asp Phe Leu Thr Gly Glu Leu
675 680 685
Leu Asn Gly Lys Arg Pro His Glu Leu Leu Leu Ile Gln Glu Leu Met
690 695 700
Lys Gln Pro Glu Thr Ser Thr Glu Glu Phe Arg Lys Leu Leu Gln Ala
705 710 715 720
Gln Ser Thr Ser Ser Asp Glu Gln Thr Ile Asn Ser Val Glu Arg Ile
725 730 735
Leu Ser Gln Glu Phe Tyr Thr Gly Pro Asn Arg Lys Lys Phe Gly Glu
740 745 750
His Pro Ile Leu Ser Val Gln Asn Arg Thr Tyr Ser Phe Thr Pro Glu
755 760 765
Phe Arg Arg Ala Leu Asp Glu Ser Leu Glu Val Thr Asp Arg Asp Glu
770 775 780
Ala Ala Gln Asn Phe Lys Phe His Val Gln Asp Ile Ile Asp Thr Gly
785 790 795 800
Leu Phe Ile Ala Arg Asn Asn Gly Phe Trp Gln Gly Asn Leu Val Val
805 810 815
Gly Glu Arg Tyr Ser Arg Arg Asp Val Cys Arg Ile Leu Asn Trp Glu
820 825 830
Arg Asn Asn Glu Ser Thr Ile Tyr Gly Tyr Lys Val Asp Ser Tyr Thr
835 840 845
Ser Thr Cys Pro Ile Phe Val Thr Tyr His Lys Ala Asp Asp Val Ser
850 855 860
Glu Ser Thr Arg Tyr Gln Asp Glu Leu Val Asp Pro Asn Thr Leu His
865 870 875 880
Trp Tyr Ser Arg Gly Asn Arg Lys Ile Thr Ser Asn Glu Ile Lys Pro
885 890 895
Ile Ala Ala Asn Ala Val Asp Leu His Val Phe Val Lys Lys Asp Asp
900 905 910
Ala Glu Gly Leu Asp Phe Phe Tyr Leu Gly Gln Ala His Ser Glu Asn
915 920 925
Ser Lys Gln Ser Ser Met Pro Gly Asn Lys Gly Val Val Gln Pro Val
930 935 940
Val Thr Met Asp Leu Gln Phe Asp Thr Pro Val Glu Gln Ser Leu Phe
945 950 955 960
Glu Tyr Leu Ser Thr Asn Leu Ala Val Thr Glu
965 970
<210>5
<211>934
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(904)
<223>RXA00056
<400>5
caccacaggc ttccggaagt tgcacattgg aaaaaccctt agataaatat tgaaatatga 60
atttaataat agattccgaa cgaaatcggt gttagcgtct gtg ctg aaa caa tct 115
Val Leu Lys Gln Ser
1 5
aat cgc gtt tca gga cac cta cat gat cag gag ctc ttt ttg tta aac 163
Asn Arg Val Ser Gly His Leu His Asp Gln Glu Leu Phe Leu Leu Asn
10 15 20
aga gtc agt cgt att gca ggc gct tct gca atc aca cta tgc atc ggc 211
Arg Val Ser Arg Ile Ala Gly Ala Ser Ala Ile Thr Leu Cys Ile Gly
25 30 35
tta acc aca ata cta agc cct act tcc act gca caa agc ctc gaa cag 259
Leu Thr Thr Ile Leu Ser Pro Thr Ser Thr Ala Gln Ser Leu Glu Gln
40 45 50
atc acc cct tta cct gaa tct gca atc gac ctc aac gcc gag att cac 307
Ile Thr Pro Leu Pro Glu Ser Ala Ile Asp Leu Asn Ala Glu Ile His
55 60 65
gta aac aca agc gac att tca gct gaa cag atc ctt ggt gct caa gat 355
Val Asn Thr Ser Asp Ile Ser Ala Glu Gln Ile Leu Gly Ala Gln Asp
70 75 80 85
gaa atc aca act atg tac gat tct cat gac ccc tac gag tac ttc gat 403
Glu Ile Thr Thr Met Tyr Asp Ser His Asp Pro Tyr Glu Tyr Phe Asp
90 95 100
acc ctc acc gac atc gaa cag cgt tca ata ata gca gcg ctt aaa cgg 451
Thr Leu Thr Asp Ile Glu Gln Arg Ser Ile Ile Ala Ala Leu Lys Arg
105 110 115
gat ccg agt tca ctc caa csa cgc caa gaa acc cgt ctc gcg gca cag 499
Asp Pro Ser Ser Leu Gln Gln Arg Gln Glu Thr Arg Leu Ala Ala Gln
120 125 130
tcc gac ccc tac aaa att tac ata tca ggc ctc gaa atg ctt tca tgc 547
Ser Asp Pro Tyr Lys Ile Tyr Ile Ser Gly Leu Glu Met Leu Ser Cys
135 140 145
atc aat cta gtt gat gtt gta tca tgc ggg att gca aac caa gca gca 595
Ile Asn Leu Val Asp Val Val Ser Cys Gly Ile Ala Asn Gln Ala Ala
150 155 160 165
acc aaa gca aat aat gag gct gtc gca cga tac cca ggc gat tcc ctt 643
Thr Lys Ala Asn Asn Glu Ala Val Ala Arg Tyr Pro Gly Asp Ser Leu
170 175 180
cgc aac ggc aaa ggc gat gca ttt cgg cat tgc tca tgg sac gct ctg 691
Arg Asn Gly Lys Gly Asp Ala Phe Arg His Cys Ser Trp Asn A1a Leu
185 190 195
atg acg ata cga atc ggg agc aat gga gct gaa aga att gca aca aac 739
Met Thr Ile Arg Ile Gly Ser Asn Gly Ala Glu Arg Ile Ala Thr Asn
200 205 210
cac gag aca atc ggg gac ggt ccg gcc gat gaa aat gca atg gac cta 787
His Glu Thr Ile Gly Asp Gly Pro Ala Asp Glu Asn Ala Met Asp Leu
215 220 225
ttc aat aat gca caa ggc cga cag atc gga gcc gga ttc att aat agt 835
Phe Asn Asn Ala Gln Gly Arg Gln Ile Gly Ala Gly Phe Ile Asn Ser
230 235 240 245
aag gat gaa act agc gcg ctc gcg ata tgc gcg ctg tgg aca aat ctc 883
Lys Asp Glu Thr Ser Ala Leu Ala Ile Cys Ala Leu Trp Thr Asn Leu
250 255 260
ggt aga cta aaa act cta aaataagcaaggt gccctctgat gctaaaaaag 934
Gly Arg Leu Lys Thr Leu Lys
265
<210>6
<211>268
<212>PRT
<213>谷氨酸棒状杆菌
<400>6
Val Leu Lys Gln Ser Asn Arg Val Ser Gly His Leu His Asp Gln Glu
1 5 10 15
Leu Phe Leu Leu Asn Arg Val Ser Arg Ile Ala Gly Ala Ser Ala Ile
20 25 30
Thr Leu Cys Ile Gly Leu Thr Thr Ile Leu Ser Pro Thr Ser Thr Ala
35 40 45
Gln Ser Leu Glu Gln Ile Thr Pro Leu Pro Glu Ser Ala Ile Asp Leu
50 55 60
Asn Ala Glu Ile His Val Asn Thr Ser Asp Ile Ser Ala Glu Gln Ile
65 70 75 80
Leu Gly Ala Gln Asp Glu Ile Thr Thr Met Tyr Asp Ser His Asp Pro
85 90 95
Tyr Glu Tyr Phe Asp Thr Leu Thr Asp Ile Glu Gln Arg Ser Ile Ile
100 105 110
Ala Ala Leu Lys Arg Asp Pro Ser Ser Leu Gln Gln Arg Gln Glu Thr
115 120 125
Arg Leu Ala Ala Gln Ser Asp Pro Tyr Lys Ile Tyr Ile Ser Gly Leu
130 135 140
Glu Met Leu Ser Cys Ile Asn Leu Val Asp Val Val Ser Cys Gly Ile
145 150 155 160
Ala Asn Gln Ala Ala Thr Lys Ala Asn Asn Glu Ala Val Ala Arg Tyr
165 170 175
Pro Gly Asp Ser Leu Arg Asn Gly Lys Gly Asp Ala Phe Arg His Cys
180 185 190
Ser Trp Asn Ala Leu Met Thr Ile Arg Ile Gly Ser Asn Gly Ala Glu
195 200 205
Arg Ile Ala Thr Asn His Glu ThrIle Gly Asp Gly Pro Ala Asp Glu
210 215 220
Asn Ala Met Asp Leu Phe Asn Asn Ala Gln Gly Arg Gln Ile Gly Ala
225 230 235 240
Gly Phe Ile Asn Ser Lys Asp Glu Thr Ser Ala Leu Ala Ile Cys Ala
245 250 255
Leu Trp Thr Asn Leu Gly Arg Leu Lys Thr Leu Lys
260 265
<210>7
<211>760
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(730)
<223>RXA00065
<400>7
tggcaccgtc gctgcctcca gttgaggaac ttgaggttgc catggatggc gtggctacct 60
gtgtgctgtt ggcagcagcg gagcattacg ctaactaaaa gtg aat aca gcg gag 115
Val Asn Thr Ala Glu
1 5
aca gca cat tgg att aac cgt ctg ctt cct ggg aca ccg gaa ttc cac 163
Thr Ala His Trp Ile Asn Arg Leu Leu Pro Gly Thr Pro Glu Phe His
10 15 20
cag gtt ggc gcg ttt aaa gtg gca ggt tac acg ctt gag gat gaa tca 211
Gln Val Gly Ala Phe Lys Val Ala Gly Tyr Thr Leu Glu Asp Glu Ser
25 30 35
att gcg tgt tct gtc aat ttc ggg cgc gtc aac acc ggg ctg gtc acg 259
Ile Ala Cys Ser Val Asn Phe Gly Arg Val Asn Thr Gly Leu Val Thr
40 45 50
gaa ggc ggc gcg gaa acg gtc gat gtg cga agt gag att ttg agc ctg 307
Glu Gly Gly Ala Glu Thr Val Asp Val Arg Ser Glu Ile Leu Ser Leu
55 60 65
gcc agg gcc gac gtg tcc gtg cct ggg cgc gcc gtc ggc gct gct gca 355
Ala Arg Ala Asp Val Ser Val Pro Gly Arg Ala Val Gly Ala Ala Ala
70 75 80 85
aca atg ctt ctc gac gcc tcc ctc tcc ttc aaa tcc gcc acc gat tcc 403
Thr Met Leu Leu Asp Ala Ser Leu Ser Phe Lys Ser Ala Thr Asp Ser
90 95 100
agc gtc act ccc atg cat gcc caa ccg gga cag atc ttg ccg tgc gta 451
Ser Val Thr Pro Met His Ala Gln Pro Gly Gln Ile Leu Pro Cys Val
105 110 115
gga att atg gcg aat ctt ccc caa gag ggt ttt tct gtg gtg cac gga 499
Gly Ile Met Ala Asn Leu Pro Gln Glu Gly Phe Ser Val Val His Gly
120 125 130
att ttg gcg gat ccc cgc att tgg ggc ccg gag att ccg tat gtt cgt 547
Ile Leu Ala Asp Pro Arg Ile Trp Gly Pro Glu Ile Pro Tyr Val Arg
135 140 145
gaa gaa gca ggt caa gta aat gtg gaa gca ccc gac gag gct ggc gat 595
Glu Glu Ala Gly Gln Val Asn Val Glu Ala Pro Asp Glu Ala Gly Asp
150 155 160 165
cta gcc cga ttg acg ttg cct ctg cag ttg atc ctg ctc acg gag gaa 643
Leu Ala Arg Leu Thr Leu Pro Leu Gln Leu Ile Leu Leu Thr Glu Glu
170 175 180
gag ttt gcc atc gct ctc aac gag ggc agc gac gtc atg ttc gaa cga 691
Glu Phe Ala Ile Ala Leu Asn Glu Gly Ser Asp Val Met Phe Glu Arg
185 190 195
atg gcg gag caa gaa gtc gat ctt ctg gat ctg aaa cgt taagacggta 740
Met Ala Glu Gln Glu Val Asp Leu Leu Asp Leu Lys Arg
200 205 210
ggctcaattc cgtggctttg 760
<210>8
<211>210
<212>PRT
<213>谷氨酸棒状杆菌
<400>8
Val Asn Thr Ala Glu Thr Ala His Trp Ile Asn Arg Leu Leu Pro Gly
1 5 10 15
Thr Pro Glu Phe His Gln Val Gly Ala Phe Lys Val Ala Gly Tyr Thr
20 25 30
Leu Glu Asp Glu Ser Ile Ala Cys Ser Val Asn Phe Gly Arg Val Asn
35 40 45
Thr Gly Leu Val Thr Glu Gly Gly Ala Glu Thr Val Asp Val Arg Ser
50 55 60
Glu Ile Leu Ser Leu Ala Arg Ala Asp Val Ser Val Pro Gly Arg Ala
65 70 75 80
Val Gly Ala Ala Ala Thr Met Leu Leu Asp Ala Ser Leu Ser Phe Lys
85 90 95
Ser Ala Thr Asp Ser Ser Val Thr Pro Met His Ala Gln Pro Gly Gln
100 105 110
Ile Leu Pro Cys Val Gly Ile Met Ala Asn Leu Pro Gln Glu Gly Phe
115 120 125
Ser Val Val His Gly Ile Leu Ala Asp Pro Arg Ile Trp Gly Pro Glu
130 135 140
Ile Pro Tyr Val Arg Glu Glu Ala Gly Gln Val Asn Val Glu Ala Pro
145 150 155 160
Asp Glu Ala Gly Asp Leu Ala Arg Leu Thr Leu Pro Leu Gln Leu Ile
165 170 175
Leu Leu Thr Glu Glu Glu Phe Ala Ile Ala Leu Asn Glu Gly Ser Asp
180 185 190
Val Met Phe Glu Arg Met Ala Glu Gln Glu Val Asp Leu Leu Asp Leu
195 200 205
Lys Ar9
210
<210>9
<211>712
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(682)
<223>RXA00068
<400>9
ggctgctaaa cggcaaccaa tagagcgata attcgctaag gcgaatgtaatcgcagcaac 60
atatagcacc ggcttaacag gccggtgcta ttctgttcgc atg act tcg aag gat 115
Met Thr Ser Lys Asp
1 5
ctg att gtg acc tcc tat acg tct tgg ggc aag cgt ttc aag aat gac 163
Leu Ile Val Thr Ser Tyr Thr Ser Trp Gly Lys Arg Phe Lys Asn Asp
10 15 20
ggg aag ctt ttt att aac cta ctt cgc agc acc act gat agt gct gat 211
Gly Lys Leu Phe Ile Asn Leu Leu Arg Ser Thr Thr Asp Ser Ala Asp
25 30 35
gaa aag gtt tta gcc act ttc ggt gaa gtt ccc agc aaa tca ttt gaa 259
Glu Lys Val Leu Ala Thr Phe Gly Glu Val Pro Ser Lys Ser Phe Glu
40 45 50
acc acc gca acg gtt gat gag cag cag tgg gaa ctg tcc ttc agt att 307
Thr Thr Ala Thr Val Asp Glu Gln Gln Trp Glu Leu Ser Phe Ser Ile
55 60 65
gat gga acg gca act gcc aag ctt cct gat ggt cgt gtg ttc agc gcg 355
Asp Gly Thr Ala Thr Ala Lys Leu Pro Asp Gly Arg Val Phe Ser Ala
70 75 80 85
aat gca ggt gag aag acc ttt acc aag tcc aag cgg att gaa atc gsc 403
Asn Ala Gly Glu Lys Thr Phe Thr Lys Ser Lys Arg Ile Glu Ile Asp
90 95 100
atg gac ggc acc gcg atg gct gct gtt aat gaa gat aaa aac aat tgg 451
Met Asp Gly Thr Ala Met Ala Ala Val Asn Glu Asp Lys Asn Asn Trp
105 110 115
att atc gac gat tct gaa gag aat aaa gtc gct cag ttt acc ggt atg 499
Ile Ile Asp Asp Ser Glu Glu Asn Lys Val Ala Gln Phe Thr Gly Met
120 125 130
aac aac ggt gtg cgt cgc gcg att gtg gag ttt gag cct gac gta gaa 547
Asn Asn Gly Val Arg Arg Ala Ile Val Glu Phe Glu Pro Asp Val Glu
135 140 145
gtc acc cag gag cag gaa att ttc ttg tcg tgg gtt gct cgg aaa act 595
Val Thr Gln Glu Gln Glu Ile Phe Leu Ser Trp Val Ala Arg Lys Thr
150 155 160 165
ctg gaa tcc cgc atg ttg ggc tcc agt tgg gga ctg act ctg ttt ttg 643
Leu Glu Ser Arg Met Leu Gly Ser Ser Trp Gly Leu Thr Leu Phe Leu
170 175 180
atc att ttg acg cca atc att att ttc ctc act ttc agc taaaaggacc 692
Ile Ile Leu Thr Pro Ile Ile Ile Phe Leu Thr Phe Ser
185 190
atgcaatggt agacgctcag 712
<210>10
<211>194
<212>PRT
<213>谷氨酸棒状杆菌
<400>10
Met Thr Ser Lys Asp Leu Ile Val Thr Ser Tyr Thr Ser Trp Gly Lys
1 5 10 15
Arg Phe Lys Asn Asp Gly Lys Leu Phe Ile Asn Leu Leu Arg Ser Thr
20 25 30
Thr Asp Ser Ala Asp Glu Lys Val Leu Ala Thr Phe Gly Glu Val Pro
35 40 45
Ser Lys Ser Phe Glu Thr Thr Ala Thr Val Asp Glu Gln Gln Trp Glu
50 55 60
Leu Ser Phe Ser Ile Asp Gly Thr Ala Thr Ala Lys Leu Pro Asp Gly
65 70 75 80
Arg Val Phe Ser Ala Asn Ala Gly Glu Lys Thr Phe Thr Lys Ser Lys
85 90 95
Arg Ile Glu Ile Asp Met Asp Gly Thr Ala Met Ala Ala Val Asn Glu
100 105 110
Asp Lys Asn Asn Trp Ile Ile Asp Asp Ser Glu Glu Asn Lys Val Ala
115 120 125
Gln Phe Thr Gly Met Asn Asn Gly Val Arg Arg Ala Ile Val Glu Phe
130 135 140
Glu Pro Asp Val Glu Val Thr Gln Glu Gln Glu Ile Phe Leu Ser Trp
145 150 155 160
Val Ala Arg Lys Thr Leu Glu Ser Arg Met Leu Gly Ser Ser Trp Gly
165 170 175
Leu Thr Leu Phe Leu Ile Ile Leu Thr Pro Ile Ile Ile Phe Leu Thr
180 185 190
Phe Ser
<210>11
<211>1492
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1462)
<223>RXA00077
<400>11
ctaaattgtt ttaacgcgtg aagcagtccc cgcccgattt attcgaggcg gggactttcg 60
ctttccggga taaaaattgc aacgcactac actgagcagt atg aat gat gag aat 115
Met Asn Asp Glu Asn
1 5
att caa agc tcc aac tat cag cca ttc ccg agt ttt gac gat tgg aaa 163
Ile Gln Ser Ser Asn Tyr Gln Pro Phe Pro Ser Phe Asp Asp Trp Lys
10 15 20
cag atc gag gtg tcg ctc tta gat gtc atc gaa tcc tca cgc cat ttt 211
Gln Ile Glu Val Ser Leu Leu Asp Val Ile Glu Ser Ser Arg His Phe
25 30 35
tct gat ttg aaa gat agc act gat cgt tct gcg tta gat gct gcg cta 259
Ser Asp Leu Lys Asp Ser Thr Asp Arg Ser Ala Leu Asp Ala Ala Leu
40 45 50
gag aga gca aaa aga gct gcc gca gtt gat acc aat gcc ata gaa gga 307
Glu Arg Ala Lys Arg Ala Ala Ala Val Asp Thr Asn Ala Ile Glu Gly
55 60 65
atc ttc caa act gat cgc ggt ttt acc cat aca gtt gca acg cag gta 355
Ile Phe Gln Thr Asp Arg Gly Phe Thr His Thr Val Ala Thr Gln Val
70 75 80 85
ggg gct tgg gag caa caa atg gcg atg aaa ggc aaa cat gtt aag cct 403
Gly Ala Trp Glu Gln Gln Met Ala Met Lys Gly Lys His Val Lys Pro
90 95 100
gcg ttt gac gat act cta gaa ggc ttt gag tat gtt ctc gat gca gta 451
Ala Phe Asp Asp Thr Leu Glu Gly Phe Glu Tyr Val Leu Asp Ala Val
105 110 115
act ggt aga act cca atc tct cag caa tgg att aga aat ttg cac gcc 499
Thr Gly Arg Thr Pro Ile Ser Gln Gln Trp Ile Arg Asn Leu His Ala
120 125 130
gtc att ctg cgg agc caa gaa agc cac gag gtt ttt aca gcc gtt gga 547
Val Ile Leu Arg Ser Gln Glu Ser His Glu Val Phe Thr Ala Val Gly
135 140 145
gtc caa aat cag gcg ctt cag aaa ggc gag tat aaa act cag cca aat 595
Val Gln Asn Gln Ala Leu Gln Lys Gly Glu Tyr Lys Thr Gln Pro Asn
150 155 160 165
agt cca cag cgc tca gat gga tct gta cat gca tac gcc cca gtt gaa 643
Ser Pro Gln Arg Ser Asp Gly Ser Val His Ala Tyr Ala Pro Val Glu
170 175 180
gat act cct gct gaa atg gct aga ttt att tca gaa ctt gaa tct aag 691
Asp Thr Pro Ala Glu Met Ala Arg Phe Ile Ser Glu Leu Glu Ser Lys
185 190 195
gaa ttc tta gca gcc gag aag gtt att caa gct gcc tat gcc cac tat 739
Glu Phe Leu Ala Ala Glu Lys Val Ile Gln Ala Ala Tyr Ala His Tyr
200 205 210
gct ttc gta tgt att cat cct ttt gca gat ggg aat gga cga gtt gca 787
Ala Phe Val Cys Ile His Pro Phe Ala Asp Gly Asn Gly Arg Val Ala
215 220 225
cga gcc ttg gct agt gtt ttt cta tac aaa gat cct ggt gtc cct ctc 835
Arg Ala Leu Ala Ser Val Phe Leu Tyr Lys Asp Pro Gly Val Pro Leu
230 235 240 245
gta atc tac caa gat caa cgc aga gat tac atc cat gct cta gaa gca 883
Val Ile Tyr Gln Asp Gln Arg Arg Asp Tyr Ile His Ala Leu Glu Ala
250 255 260
gcg gac aag aat aac ccg ctc ctg ctg att aga ttc ttt gct gaa cga 931
Ala Asp Lys Asn Asn Pro Leu Leu Leu Ile Arg Phe Phe Ala Glu Arg
265 270 275
gtg acc gat act att aac tct att atc gtt gat ctc act acc ccg atc 979
Val Thr Asp Thr Ile Asn Ser Ile Ile Val Asp Leu Thr Thr Pro Ile
280 285 290
gcg ggt aaa tct ggt tcg gct aag ctt tcg gat gcg cta cgc ccc act 1027
Ala Gly Lys Ser Gly Ser Ala Lys Leu Ser Asp Ala Leu Arg Pro Thr
295 300 305
cgc gta tta cca gaa tta cat gat gct gca cat agg ctc caa gaa agt 1075
Arg Val Leu Pro Glu Leu His Asp Ala Ala His Arg Leu Gln Glu Ser
310 315 320 325
tta ttt aca gaa atc cga tct cga ttg gat gaa gaa gga aaa agg aat 1123
Leu Phe Thr Glu Ile Arg Ser Arg Leu Asp Glu Glu Gly Lys Arg Asn
330 335 340
ggg ttg gag ttt cta ctt caa cgg att ttt atc ggt tcc cca ttc aat 1171
Gly Leu Glu Phe Leu Leu Gln Arg Ile Phe Ile Gly Set Pro Phe Asn
345 350 355
ctg cca gag ggc tat aac gct ttc cct gat agc tat tgt ctg acc tta 1219
Leu Pro Glu Gly Tyr Asn Ala Phe Pro Asp Ser Tyr Cys Leu Thr Leu
360 365 370
gct ttc aat agc aac tct cca aaa caa atc ttc cac ccg cta tcc ata 1267
Ala Phe Asn Ser Asn Ser Pro Lys Gln Ile Phe His Pro Leu Ser Ile
375 380 385
gta ata gca gct cga gat ggg aaa aga gcg agc agc gac ctc gtg gca 1315
Val Ile Ala Ala Arg Asp Gly Lys Arg Ala Ser Ser Asp Leu Val Ala
390 395 400 405
gct act tct att gga tac aac ttt cac gct tac gga cgt gaa gtc gag 1363
Ala Thr Ser Ile Gly Tyr Asn Phe His Ala Tyr Gly Arg Glu Val Glu
410 415 420
cct gtt gtt act gaa agc ttt cga gaa cgt gtg aaa att tac gcc gac 1411
Pro Val Val Thr Glu Ser Phe Arg Glu Arg Val Lys Ile Tyr Ala Asp
425 430 435
ggg att gta gat cac ttc tta acc gaa ctg gct aaa aag ttt caa cag 1459
Gly Ile Val Asp His Phe Leu Thr Glu Leu Ala Lys Lys Phe Gln Gln
440 445 450
aat taattagcct atctcggctt tcgccttccg 1492
Asn
<210>12
<211>454
<212>PRT
<213>谷氨酸棒状杆菌
<400>12
Met Asn Asp Glu Asn Ile Gln Ser Ser Asn Tyr Gln Pro Phe Pro Ser
1 5 10 15
Phe Asp Asp Trp Lys Gln Ile Glu Val Ser Leu Leu Asp Val Ile Glu
20 25 30
Ser Ser Arg His Phe Ser Asp Leu Lys Asp Ser Thr Asp Arg Ser Ala
35 40 45
Leu Asp Ala Ala Leu Glu Arg Ala Lys Arg Ala Ala Ala Val Asp Thr
50 55 60
Asn Ala Ile Glu Gly Ile Phe Gln Thr Asp Arg Gly Phe Thr His Thr
65 70 75 80
Val Ala Thr Gln Val Gly Ala Trp Glu Gln Gln Met Ala Met Lys Gly
85 90 95
Lys His Val Lys Pro Ala Phe Asp Asp Thr Leu Glu Gly Phe Glu Tyr
100 105 110
Val Leu Asp Ala Val Thr Gly Arg Thr Pro Ile Ser Gln Gln Trp Ile
115 120 125
Arg Asn Leu His Ala Val Ile Leu Arg Ser Gln Glu Ser His Glu Val
130 135 140
Phe Thr Ala Val Gly Val Gln Asn Gln Ala Leu Gln Lys Gly Glu Tyr
145 150 155 160
Lys Thr Gln Pro Asn Ser Pro Gln Arg Ser Asp Gly Ser Val His Ala
165 170 175
Tyr Ala Pro Val Glu Asp Thr Pro Ala Glu Met Ala Arg Phe Ile Ser
180 185 190
Glu Leu Glu Ser Lys Glu Phe Leu Ala Ala Glu Lys Val Ile Gln Ala
195 200 205
Ala Tyr Ala His Tyr Ala Phe Val Cys Ile His Pro Phe Ala Asp Gly
210 215 220
Asn Gly Arg Val Ala Arg Ala Leu Ala Ser Val Phe Leu Tyr Lys Asp
225 230 235 240
Pro Gly Val Pro Leu Val Ile Tyr Gln Asp Gln Arg Arg Asp Tyr Ile
245 250 255
His Ala Leu Glu Ala Ala Asp Lys Asn Asn Pro Leu Leu Leu Ile Arg
260 265 270
Phe Phe Ala Glu Arg Val Thr Asp Thr Ile Asn Ser Ile Ile Val Asp
275 280 285
Leu Thr Thr Pro Ile Ala Gly Lys Ser Gly Ser Ala Lys Leu Ser Asp
290 295 300
Ala Leu Arg Pro Thr Arg Val Leu Pro Glu Leu His Asp Ala Ala His
305 310 315 320
Arg Leu Gln Glu Ser Leu Phe Thr Glu Ile Arg Ser Arg Leu Asp Glu
325 330 335
Glu Gly Lys Arg Asn Gly Leu Glu Phe Leu Leu Gln Arg Ile Phe Ile
340 345 350
Gly Ser Pro Phe Asn Leu Pro Glu Gly Tyr Asn Ala Phe Pro Asp Ser
355 360 365
Tyr Cys Leu Thr Leu Ala Phe Asn Ser Asn Ser Pro Lys Gln Ile Phe
370 375 380
His Pro Leu Ser Ile Val Ile Ala Ala Arg Asp Gly Lys Arg Ala Ser
385 390 395 400
Ser Asp Leu Val Ala Ala Thr Ser Ile Gly Tyr Asn Phe His Ala Tyr
405 410 415
Gly Arg Glu Val Glu Pro Val Val Thr Glu Ser Phe Arg Glu Arg Val
420 425 430
Lys Ile Tyr Ala Asp Gly Ile Val Asp His Phe Leu Thr Glu Leu Ala
435 440 445
Lys Lys Phe Gln Gln Asn
450
<210>13
<211>1660
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1630)
<223>RXA00080
<400>13
atgttcttgt ttccctcatg atgagctccg tggtctacct cgcattcgat gtgggcttgg 60
gacttaatct tccttccgga cttttgggtg gtggctttta atg gat att ttg tcc 115
Met Asp Ile Leu Ser
1 5
ctc ttg atg gaa ggt ttc gcc ggc gcg cta acg ccg atg aac ctc ctc 163
Leu Leu Met Glu Gly Phe Ala Gly Ala Leu Thr Pro Met Asn Leu Leu
10 15 20
tgg gtg att gtc ggc tgt ttg ctc ggc acc gcg gtt ggc gtc atg cct 211
Trp Val Ile Val Gly Cys Leu Leu Gly Thr Ala Val Gly Val Met Pro
25 30 35
ggt ctt gga tcc tcc atg gct gtg gcg ctg ctg ctg cca atg acc ttc 259
Gly Leu Gly Ser Ser Met Ala Val Ala Leu Leu Leu Pro Met Thr Phe
40 45 50
gcg ctt gat cca act gcc gcg ttc att atg ttc tct ggc gta tat ttc 307
Ala Leu Asp Pro Thr Ala Ala Phe Ile Met Phe Ser Gly Val Tyr Phe
55 60 65
ggt ggt ctc ttc ggt gac tcc acg atg gca att ttg atg aac acc cca 355
Gly Gly Leu Phe Gly Asp Ser Thr Met Ala Ile Leu Met Asn Thr Pro
70 75 80 85
ggt cag gca tcg gca atc gcc tca aca ttc gag ggc cac cgc atg gct 403
Gly Gln Ala Ser Ala Ile Ala Ser Thr Phe Glu Gly His Arg Met Ala
90 95 100
ctt aac ggc cgt gcg cca cag gct ctg gct acc gca gcg atc ggt gcc 451
Leu Asn Gly Arg Ala Pro Gln Ala Leu Ala Thr Ala Ala Ile Gly Ala
105 110 115
ttc atc ggc ggt atc gtc tcc tcc ttc atc gtg gtc ttc ctc gca cca 499
Phe Ile Gly Gly Ile Val Ser Ser Phe Ile Val Val Phe Leu Ala Pro
120 125 130
acc ctg gcg gaa ctg tcc acc gca ttc ggc ccc gcc gag tac ttc gca 547
Thr Leu Ala Glu Leu Ser Thr Ala Phe Gly Pro Ala Glu Tyr Phe Ala
135 140 145
ctg gca ctc ttc gcg ttc gtc gcc acc tcc tcc gtg gtg tcc gac tcc 595
Leu Ala Leu Phe Ala Phe Val Ala Thr Ser Ser Val Val Ser Asp Ser
150 155 160 165
gtg ttt aag gga ctt gcg tcc ctc att ttc ggc ctc ggc att gcg acc 643
Val Phe Lys Gly Leu Ala Ser Leu Ile Phe Gly Leu Gly Ile Ala Thr
170 175 180
atc ggc atc gat tcg gtc acc ggc atc gag cgc ttc acg ctc ggg gca 691
Ile Gly Ile Asp Ser Val Thr Gly Ile Glu Arg Phe Thr Leu Gly Ala
185 190 195
ccg cag ctt ttc gac gga att tcc ctc gtt act gtt acc gtc gcg att 739
Pro Gln Leu Phe Asp Gly Ile Ser Leu Val Thr Val Thr Val Ala Ile
200 205 210
ttg gca ctg gga gaa gtg ttt tac att gca gcc cgc gca cgc cgt gac 787
Leu Ala Leu Gly Glu Val Phe Tyr Ile Ala Ala Arg Ala Arg Arg Asp
215 220 225
aaa gca aat ctg gag acg cgc tct gca ggc cgt ccg tgg ctt acc gga 835
Lys Ala Asn Leu Glu Thr Arg Ser Ala Gly Arg Pro Trp Leu Thr Gly
230 235 240 245
acg gaa ttc aaa gaa gcc gct cca gcc tgg gca cgc gga acc atc att 883
Thr Glu Phe Lys Glu Ala Ala Pro Ala Trp Ala Arg Gly Thr Ile Ile
250 255 260
ggt ctg cct ttc ggt gtg atc cct gtt ggt gga tct gaa gtt cca acc 931
Gly Leu Pro Phe Gly Val Ile Pro Val Gly Gly Ser Glu Val Pro Thr
265 270 275
ttc ttg gct tac tcc acc gag cgc gca ttg gat aaa cga cgc aaa gat 979
Phe Leu Ala Tyr Ser Thr Glu Arg Ala Leu Asp Lys Arg Arg Lys Asp
280 285 290
ccg cag ttc ggc gat aaa ggt gca atc cga gga ctc gct gct cct gaa 1027
Pro Gln Phe Gly Asp Lys Gly Ala Ile Arg Gly Leu Ala Ala Pro Glu
295 300 305
gct gca ggt aac gcc acc aca ggc atg gcg atg ggc gct ctt ctt gcc 1075
Ala Ala Gly Asn Ala Thr Thr Gly Met Ala Met Gly Ala Leu Leu Ala
310 315 320 325
ctg ggt ctc cca gtc tct gca act gcg gcc atc atg ttg gca gcg ttc 1123
Leu Gly Leu Pro Val Ser Ala Thr Ala Ala Ile Met Leu Ala Ala Phe
330 335 340
cgc cag tac gga atc cag ccc gga cca cta ctc ttc gat cgc aac cct 1171
Arg Gln Tyr Gly Ile Gln Pro Gly Pro Leu Leu Phe Asp Arg Asn Pro
345 350 355
gaa ctt gtc tgg gca ctt ctt gcc agc ttc ttc atc gcg atg atc gtc 1219
Glu Leu Val Trp Ala Leu Leu Ala Ser Phe Phe Ile Ala Met Ile Val
360 365 370
ctg ctg ttc atc aac ctg ccg ttc gca cag ctg tgg gca aag ctc ctg 1267
Leu Leu Phe Ile Asn Leu Pro Phe Ala Gln Leu Trp Ala Lys Leu Leu
375 380 385
ctc att cca aac cac tac ctc tac tcc ggc atc gca ttg ttc tgt ggc 1315
Leu Ile Pro Asn His Tyr Leu Tyr Ser Gly Ile Ala Leu Phe Cys Gly
390 395 400 405
ctg ggc att tac gcc acc tcc ggc gca gtg ttc gac ctg ctc atg ctg 1363
Leu Gly Ile Tyr Ala Thr Ser Gly Ala Val Phe Asp Leu Leu Met Leu
410 415 420
ctc ggc atc ggt gtc gtg gct ttg atc atg cgt cgc tac ggt tac ccg 1411
Leu Gly Ile Gly Val Val Ala Leu Ile Met Arg Arg Tyr Gly Tyr Pro
425 430 435
ctg gca ccg ctg atg atc ggt atg gtt ctt gga cct ttg gct gaa acc 1459
Leu Ala Pro Leu Met Ile Gly Met Val Leu Gly Pro Leu Ala Glu Thr
440 445 450
tcc ctc cgc gac gca cta ctg tcc tcg gtt ggc gat ttc tcc atc ctc 1507
Ser Leu Arg Asp Ala Leu Leu Ser Ser Val Gly Asp Phe Ser Ile Leu
455 460 465
gtc tcc agc ccc atc acc tgg tcc ctc tac gca gtg ctc gcc atc ttc 1555
Val Ser Ser Pro Ile Thr Trp Ser Leu Tyr Ala Val Leu Ala Ile Phe
470 475 480 485
atc gcg gtc agt gtc atc act gca atc cgc ggt cgt cgc aag cac ctg 1603
Ile Ala Val Ser Val Ile Thr Ala Ile Arg Gly Arg Arg Lys His Leu
490 495 500
act tct cag ctc gaa acc atc gac gct taaagtcccc gtatagaaac 1650
Thr Ser Gln Leu Glu Thr Ile Asp Ala
505 510
aggcaaaccg 1660
<210>14
<211>510
<212>PRT
<213>谷氨酸棒状杆菌
<400>14
Met Asp Ile Leu Ser Leu Leu Met Glu Gly Phe Ala Gly Ala Leu Thr
1 5 10 15
Pro Met Asn Leu Leu Trp Val Ile Val Gly Cys Leu Leu Gly Thr Ala
20 25 30
Val Gly Val Met Pro Gly Leu Gly Ser Ser Met Ala Val Ala Leu Leu
35 40 45
Leu Pro Met Thr Phe Ala Leu Asp Pro Thr Ala Ala Phe Ile Met Phe
50 55 60
Ser Gly Val Tyr Phe Gly Gly Leu Phe Gly Asp Ser Thr Met Ala Ile
65 70 75 80
Leu Met Asn Thr Pro Gly Gln Ala Ser Ala Ile Ala Ser Thr Phe Glu
85 90 95
Gly His Arg Met Ala Leu Asn Gly Arg Ala Pro Gln Ala Leu Ala Thr
100 105 110
Ala Ala Ile Gly Ala Phe Ile Gly Gly Ile Val Ser Ser Phe Ile Val
115 120 125
Val Phe Leu Ala Pro Thr Leu Ala Glu Leu Ser Thr Ala Phe Gly Pro
130 135 140
Ala Glu Tyr Phe Ala Leu Ala Leu Phe Ala Phe Val Ala Thr Ser Ser
145 150 155 160
Val Val Ser Asp Ser Val Phe Lys Gly Leu Ala Ser Leu Ile Phe Gly
165 170 175
Leu Gly Ile Ala Thr Ile Gly Ile Asp Ser Val Thr Gly Ile Glu Arg
180 185 190
Phe Thr Leu Gly Ala Pro Gln Leu Phe Asp Gly Ile Ser Leu Val Thr
195 200 205
Val Thr Val Ala Ile Leu Ala Leu Gly Glu Val Phe Tyr Ile Ala Ala
210 215 220
Arg Ala Arg Arg Asp Lys Ala Asn Leu Glu Thr Arg Ser Ala Gly Arg
225 230 235 240
Pro Trp Leu Thr Gly Thr Glu Phe Lys Glu Ala Ala Pro Ala Trp Ala
245 250 255
Arg Gly Thr Ile Ile Gly Leu Pro Phe Gly Val Ile Pro Val Gly Gly
260 265 270
Ser Glu Val Pro Thr Phe Leu Ala Tyr Ser Thr Glu Arg Ala Leu Asp
275 280 285
Lys Arg Arg Lys Asp Pro Gln Phe Gly Asp Lys Gly Ala Ile Arg Gly
290 295 300
Leu Ala Ala Pro Glu Ala Ala Gly Asn Ala Thr Thr Gly Met Ala Met
305 310 315 320
Gly Ala Leu Leu Ala Leu Gly Leu Pro Val Ser Ala Thr Ala Ala Ile
325 330 335
Met Leu Ala Ala Phe Arg Gln Tyr Gly Ile Gln Pro Gly Pro Leu Leu
340 345 350
Phe Asp Arg Asn Pro Glu Leu Val Trp Ala Leu Leu Ala Ser Phe Phe
355 360 365
Ile Ala Met Ile Val Leu Leu Phe Ile Asn Leu Pro Phe Ala Gln Leu
370 375 380
Trp Ala Lys Leu Leu Leu Ile Pro Asn His Tyr Leu Tyr Ser Gly Ile
385 390 395 400
Ala Leu Phe Cys Gly Leu Gly Ile Tyr Ala Thr Ser Gly Ala Val Phe
405 410 415
Asp Leu Leu Met Leu Leu Gly Ile Gly Val Val Ala Leu Ile Met Arg
420 425 430
Arg Tyr Gly Tyr Pro Leu Ala Pro Leu Met Ile Gly Met Val Leu Gly
435 440 445
Pro Leu Ala Glu Thr Ser Leu Arg Asp Ala Leu Leu Ser Ser Val Gly
450 455 460
Asp Phe Ser Ile Leu Val Ser Ser Pro Ile Thr Trp Ser Leu Tyr Ala
465 470 475 480
Val Leu Ala Ile Phe Ile Ala Val Ser Val Ile Thr Ala Ile Arg Gly
485 490 495
Arg Arg Lys His Leu Thr Ser Gln Leu Glu Thr Ile Asp Ala
500 505 510
<210>15
<211>679
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(649)
<223>RXA00110
<400>15
atatcgaatg tccagtcacg ctaaacttca cccaagatga gatttcgtgg cacgattggg 60
aataacgaac gctaatttta aacactggag gagcttccac gtg agc aac aaa gac 115
Val Ser Asn Lys Asp
1 5
ggc ctt ttt act gac ggt aac agc acg ttt gca cct aag gtg gat tca 163
Gly Leu Phe Thr Asp Gly Asn Ser Thr Phe Ala Pro Lys Val Asp Ser
10 15 20
att ccc ctc agc gat gtg gat acc agc gtt agc ggt gaa gcc tcc atc 211
Ile Pro Leu Ser Asp Val Asp Thr Ser Val Ser Gly Glu Ala Ser Ile
25 30 35
ggc acg ctg atc tcc aac gca acc tcc caa atg tcc agc ctt ttc cgc 259
Gly Thr Leu Ile Ser Asn Ala Thr Ser Gln Met Ser Ser Leu Phe Arg
40 45 50
gca gaa gtt gag ctg gcg aag act gaa ctc gca ggc gaa gcc aag aaa 307
Ala Glu Val Glu Leu Ala Lys Thr Glu Leu Ala Gly Glu Ala Lys Lys
55 60 65
gct gcc atc ggc ggc ggc gca ttc agc gtt gct ggc gta atc gca ctg 355
Ala Ala Ile Gly Gly Gly Ala Phe Ser Val Ala Gly Val Ile Ala Leu
70 75 80 85
tac agc tcc ttc ttc ttt ttc ttc ttc gtc gca gca ctg ctg agc gag 403
Tyr Ser Ser Phe Phe Phe Phe Phe Phe Val Ala Ala Leu Leu Ser Glu
90 95 100
tgg att aag cct tgg gca gca ttc ctc atc gtg ttc ctc ttc atg ctg 451
Trp Ile Lys Pro Trp Ala Ala Phe Leu Ile Val Phe Leu Phe Met Leu
105 110 115
gtc atc gcc gca gct ctc gca ctg ttc ggc tgg cgc aag gtg aag aag 499
Val Ile Ala Ala Ala Leu Ala Leu Phe Gly Trp Arg Lys Val Lys Lys
120 125 130
atg ggc gct ccg aag aac acc atc caa tcg gtc aac caa ctg aag aac 547
Met Gly Ala Pro Lys Asn Thr Ile Gln Ser Val Asn Gln Leu Lys Asn
135 140 145
ctg gtc cca ggt cag gca tcc gag aag ctg gag aag gcc aac aag cgt 595
Leu Val Pro Gly Gln Ala Ser Glu Lys Leu Glu Lys Ala Asn Lys Arg
150 155 160 165
ggc ctc tac acc tcc gcg tcc ttc cac agc ccc ggc gcc atc act ggc 643
Gly Leu Tyr Thr Ser Ala Ser Phe His Ser Pro Gly Ala Ile Thr Gly
170 175 180
gac cac taaaaaagga gacttcgatg gcctttttta 679
Asp His
<210>16
<211>183
<212>PRT
<213>谷氨酸棒状杆菌
<400>16
Val Ser Asn Lys Asp Gly Leu Phe Thr Asp Gly Asn Ser Thr Phe Ala
1 5 10 15
Pro Lys Val Asp Ser Ile Pro Leu Ser Asp Val Asp Thr Ser Val Ser
20 25 30
Gly Glu Ala Ser Ile Gly Thr Leu Ile Ser Asn Ala Thr Ser Gln Met
35 40 45
Ser Ser Leu Phe Arg Ala Glu Val Glu Leu Ala Lys Thr Glu Leu Ala
50 55 60
Gly Glu Ala Lys Lys Ala Ala Ile Gly Gly Gly Ala Phe Ser Val Ala
65 70 75 80
Gly Val Ile Ala Leu Tyr Ser Ser Phe Phe Phe Phe Phe Phe Val Ala
85 90 95
Ala Leu Leu Ser Glu Trp Ile Lys Pro Trp Ala Ala Phe Leu Ile Val
100 105 110
Phe Leu Phe Met Leu Val Ile Ala Ala Ala Leu Ala Leu Phe Gly Trp
115 120 125
Arg Lys Val Lys Lys Met Gly Ala Pro Lys Asn Thr Ile Gln Ser Val
130 135 140
Asn Gln Leu Lys Asn Leu Val Pro Gly Gln Ala Ser Glu Lys Leu Glu
145 150 155 160
Lys Ala Asn Lys Arg Gly Leu Tyr Thr Ser Ala Ser Phe His Ser Pro
165 170 175
Gly Ala Ile Thr Gly Asp His
180
<210>17
<211>469
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(439)
<223>RXA00118
<400>17
cgatccacga ttttagagtc tgtgcatcta cggaagtaat cggttgagcg gaagtcatac 60
cttgtccttt tcaatctcaa agtgatgggc gattccaagt gtg cca tat acc ctg 115
Val Pro Tyr Thr Leu
1 5
ggg ggt att caa gat agg ggt ggg ggt ata ctg tgg gat caa cgt tcc 163
Gly Gly Ile Gln Asp Arg Gly Gly Gly Ile Leu Trp Asp Gln Arg Ser
10 15 20
agg gca aca aaa gga gaa aaa atg caa ctc aat cct gat gag atc acc 211
Arg Ala Thr Lys Gly Glu Lys Met Gln Leu Asn Pro Asp Glu Ile Thr
25 30 35
cca gtg ctc aac cga ctc aag cgc gcc caa ggt caa ctc acc gga gta 259
Pro Val Leu Asn Arg Leu Lys Arg Ala Gln Gly Gln Leu Thr Gly Val
40 45 50
atc cga atg ctt gat gaa ggc gaa gac tgt aaa gcc gta gtc acc caa 307
Ile Arg Met Leu Asp Glu Gly Glu Asp Cys Lys Ala Val Val Thr Gln
55 60 65
ctt gcc gca gtc aca aaa gcg ctg gac agg gca ggc ttc gcc atc att 355
Leu Ala Ala Val Thr Lys Ala Leu Asp Arg Ala Gly Phe Ala Ile Ile
70 75 80 85
gcc aca ggt ttg gaa cag tgc ctc acc aac cct gat ggc gac atg gac 403
Ala Thr Gly Leu Glu Gln Cys Leu Thr Asn Pro Asp Gly Asp Met Asp
90 95 100
aaa aag gaa cta gaa aaa ctg ttc ctg tcg ctg gct taactatggc 449
Lys Lys Glu Leu Glu Lys Leu Phe Leu Ser Leu Ala
105 110
ctagttcagg gcgtttgggt 469
<210>18
<211>113
<212>PRT
<213>谷氨酸棒状杆菌
<400>18
Val Pro Tyr Thr Leu Gly Gly Ile Gln Asp Arg Gly Gly Gly Ile Leu
1 5 10 15
Trp Asp Gln Arg Ser Arg Ala Thr Lys Gly Glu Lys Met Gln Leu Asn
20 25 30
Pro Asp Glu Ile Thr Pro Val Leu Asn Arg Leu Lys Arg Ala Gln Gly
35 40 45
Gln Leu Thr Gly Val Ile Arg Met Leu Asp Glu Gly Glu Asp Cys Lys
50 55 60
Ala Val Val Thr Gln Leu Ala Ala Val Thr Lys Ala Leu Asp Arg Ala
65 70 75 80
Gly Phe Ala Ile Ile Ala Thr Gly Leu Glu Gln Cys Leu Thr Asn Pro
85 90 95
Asp Gly Asp Met Asp Lys Lys Glu Leu Glu Lys Leu Phe Leu Ser Leu
100 105 110
Ala
<210>19
<211>841
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(811)
<223>RXA00121
<400>19
gatgtccaaa tactgcgaaa ccatcgaagc gtttcacaac cgtccggcat cagctttcgg 60
cgctgtcgac cccatcttct aaacaagaaa ggcccctcca atg ttg agc gat ctg 115
Met Leu Ser Asp Leu
1 5
agc tcc att ttt gac ttc caa gac ctc tcc ggc acc ttc tct gtc gtc 163
Ser Ser Ile Phe Asp Phe Gln Asp Leu Ser Gly Thr Phe Ser Val Val
10 15 20
gac gtt ctc atc act ttg gtc ttg tcc ttc gtc ctg acc tcc atc gtg 211
Asp Val Leu Ile Thr Leu Val Leu Ser Phe Val Leu Thr Ser Ile Val
25 30 35
ggt gtg gtg tac caa aag acc cac cgc cac atc tcc tac agc cag tca 259
Gly Val Val Tyr Gln Lys Thr His Arg His Ile Ser Tyr Ser Gln Ser
40 45 50
ttc gta caa acg ttg gtg ctg gtg gga atg gtc att gca atc atc atg 307
Phe Val Gln Thr Leu Val Leu Val Gly Met Val Ile Ala Ile Ile Met
55 60 65
ctg gtg gtc ggc tcc aac att gca cgt gca ttc gcc ctg gtt ggc gcg 355
Leu Val Val Gly Ser Asn Ile Ala Arg Ala Phe Ala Leu Val Gly Ala
70 75 80 85
ctt tcg gtg att cga ttc cgc aac gca gtg aaa gaa acc cga gat gtg 403
Leu Ser Val Ile Arg Phe Arg Asn Ala Val Lys Glu Thr Arg Asp Val
90 95 100
ggc ttc ctt ttc ctt gcc atg gca atc ggc atg act tgc ggt acc cgc 451
Gly Phe Leu Phe Leu Ala Met Ala Ile Gly Met Thr Cys Gly Thr Arg
105 110 115
ttc tac gtt ctg gcg atc gct gca acc atc gtt gtc tgt ggc gtt ctg 499
Phe Tyr Val Leu Ala Ile Ala Ala Thr Ile Val Val Cys Gly Val Leu
120 125 130
ttc atc atg tac cgc ttc gac tgg ttc aag gct gat atc cag cgc cag 547
Phe Ile Met Tyr Arg Phe Asp Trp Phe Lys Ala Asp Ile Gln Arg Gln
135 140 145
gtc atc aag gtg cag gtc cca gcc gat gga caa gct gat tcc ggc agg 595
Val Ile Lys Val Gln Val Pro Ala Asp Gly Gln Ala Asp Ser Gly Arg
150 155 160 165
tcc tac gca gaa gaa gtt gaa ctg atc ctc gca cag tac tgc act tcc 643
Ser Tyr Ala Glu Glu Val Glu Leu Ile Leu Ala Gln Tyr Cys Thr Ser
170 175 180
ttt gag atg atg tcc gct gaa tct gtc cgc ggc gga gcc ctg acc gag 691
Phe Glu Met Met Ser Ala Glu Ser Val Arg Gly Gly Ala Leu Thr Glu
185 190 195
ttc tcc tac acc gct caa atg cgc aag aac gtg aag cca cat gag ctg 739
Phe Ser Tyr Thr Ala Gln Met Arg Lys Asn Val Lys Pro His Glu Leu
200 205 210
gtc gcc aag atg cgc gat gtg aac tac ggc caa aaa gcg act gtc ctg 787
Val Ala Lys Met Arg Asp Val Asn Tyr Gly Gln Lys Ala Thr Val Leu
215 220 225
acc ggt cac gat caa acg gat gta tagaaaatgc ctagctttaa atctgctcga 841
Thr Gly His Asp Gln Thr Asp Val
230 235
<210>20
<211>237
<212>PRT
<213>谷氨酸棒状杆菌
<400>20
Met Leu Ser Asp Leu Ser Ser Ile Phe Asp Phe Gln Asp Leu Ser Gly
1 5 10 15
Thr Phe Ser Val Val Asp Val Leu Ile Thr Leu Val Leu Ser Phe Val
20 25 30
Leu Thr Ser Ile Val Gly Val Val Tyr Gln Lys Thr His Arg His Ile
35 40 45
Ser Tyr Ser Gln Ser Phe Val Gln Thr Leu Val Leu Val Gly Met Val
50 55 60
Ile Ala Ile Ile Met Leu Val Val Gly Ser Asn Ile Ala Arg Ala Phe
65 70 75 80
Ala Leu Val Gly Ala Leu Ser Val Ile Arg Phe Arg Asn Ala Val Lys
85 90 95
Glu Thr Arg Asp Val Gly Phe Leu Phe Leu Ala Met Ala Ile Gly Met
100 105 110
Thr Cys Gly Thr Arg Phe Tyr Val Leu Ala Ile Ala Ala Thr Ile Val
115 120 125
Val Cys Gly Val Leu Phe Ile Met Tyr Arg Phe Asp Trp Phe Lys Ala
130 135 140
Asp Ile Gln Arg Gln Val Ile Lys Val Gln Val Pro Ala Asp Gly Gln
145 150 155 160
Ala Asp Ser Gly Arg Ser Tyr Ala Glu Glu Val Glu Leu Ile Leu Ala
165 170 175
Gln Tyr Cys Thr Ser Phe Glu Met Met Ser Ala Glu Ser Val Arg Gly
180 185 190
Gly Ala Leu Thr Glu Phe Ser Tyr Thr Ala Gln Met Arg Lys Asn Val
195 200 205
Lys Pro His Glu Leu Val Ala Lys Met Arg Asp Val Asn Tyr Gly Gln
210 215 220
Lys Ala Thr Val Leu Thr Gly His Asp Gln Thr Asp Val
225 230 235
<210>21
<211>904
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(874)
<223>RXA00150
<400>21
agctgagcag ggatcggcac tagcttctac tacgataacg agaactttgt ttcaatacat 60
tcaaaatggc ggaatatttt gatcacgggg gtgcaactgt atg gtg ttt tct atg 115
Met Val Phe Ser Met
1 5
cat gac aaa ggt gag acc cag gag aat cct gcg gac atg tca gga cgc 163
His Asp Lys Gly Glu Thr Gln Glu Asn Pro Ala Asp Met Ser Gly Arg
10 15 20
cta aac aca cca att tcc acg gtc ttt cac ttc ttt agc tca ctt ttc 211
Leu Asn Thr Pro Ile Ser Thr Val Phe His Phe Phe Ser Ser Leu Phe
25 30 35
cat gac gct ctg cgc agt gtt gcc cag tgg agt gcg tgg aag aaa atc 259
His Asp Ala Leu Arg Ser Val Ala Gln Trp Ser Ala Trp Lys Lys Ile
40 45 50
gct gta tcg gtt gtc atc gtc gca att att tcc gta act ttt ctt gtc 307
Ala Val Ser Val Val Ile Val Ala Ile Ile Ser Val Thr Phe Leu Val
55 60 65
gat gtc ccc ccg att tcg gtt tat cgt gac tgg gca aac aac gcc ggc 355
Asp Val Pro Pro Ile Ser Val Tyr Arg Asp Trp Ala Asn Asn Ala Gly
70 75 80 85
gac gct ttt gtc tta gtt ttt tgc gcc ttt tat atc ctc att act cag 403
Asp Ala Phe Val Leu Val Phe Cys Ala Phe Tyr Ile Leu Ile Thr Gln
90 95 100
ttc cct atc ccc cgc aca gtt ctc aca ctg gcc tcc ggc gtg ctg ttc 451
Phe Pro Ile Pro Arg Thr Val Leu Thr Leu Ala Ser Gly Val Leu Phe
105 110 115
ggg ccg gtt cttg ga tca gtc gtg gcg ctg ggt tcc acc aca gtg tca 499
Gly Pro Val Leu Gly Ser Val Val Ala Leu Gly Ser Thr Thr Val Ser
120 125 130
gcg gta atc tcg ctc ctc att gtt cgg ggt ctg ctt ggc gat tgg atg 547
Ala Val Ile Ser Leu Leu Ile Val Arg Gly Leu Leu Gly Asp Trp Met
135 140 145
gcg cca cgt tta acg cac ccc gca gtc tca cgc atc aat acc cga ctt 595
Ala Pro Arg Leu Thr His Pro Ala Val Ser Arg Ile Asn Thr Arg Leu
150 155 160 165
gag caa cgt gga tgg ctg gcg att acc tct tta aga atg atc gcc gct 643
Glu Gln Arg Gly Trp Leu Ala Ile Thr Ser Leu Arg Met Ile Ala Ala
170 175 180
atc ccc ttt tcc atc ctc aac tat gtt gca gcc ttg act agc gtt cct 691
Ile Pro Phe Ser Ile Leu Asn Tyr Val Ala Ala Leu Thr Ser Val Pro
185 190 195
gtt ttt tct ttt gcc atc gct acc ttg atc gga tct gca cca gga acc 739
Val Phe Ser Phe Ala Ile Ala Thr Leu Ile Gly Ser Ala Pro Gly Thr
200 205 210
atc gtc acc gtc gtt ttg gga gat gca gtc aca ggt tct gga aac tgg 787
Ile Val Thr Val Val Leu Gly Asp Ala Val Thr Gly Ser Gly Asn Trp
215 220 225
act gcc gtt gca ttc acg gta ttt tta gcg att ttg ggt gtt tta ggt 835
Thr Ala Val Ala Phe Thr Val Phe Leu Ala Ile Leu Gly Val Leu Gly
230 235 240 245
atc ttt tta gat caa aag atg cca gtc aag cct gga aag tagacaatat 884
Ile Phe Leu Asp Gln Lys Met Pro Val Lys Pro Gly Lys
250 255
aaggtagggt tggagaagat 904
<210>22
<211>258
<212>PRT
<213>谷氨酸棒状杆菌
<400>22
Met Val Phe Ser Met His Asp Lys Gly Glu Thr Gln Glu Asn Pro Ala
1 5 10 15
Asp Met Ser Gly Arg Leu Asn Thr Pro Ile Ser Thr Val Phe His Phe
20 25 30
Phe Ser Ser Leu Phe His Asp Ala Leu Arg Ser Val Ala Gln Trp Ser
35 40 45
Ala Trp Lys Lys Ile Ala Val Ser Val Val Ile Val Ala Ile Ile Ser
50 55 60
Val Thr Phe Leu Val Asp Val Pro Pro Ile Ser Val Tyr Arg Asp Trp
65 70 75 80
Ala Asn Asn Ala Gly Asp Ala Phe Val Leu Val Phe Cys Ala Phe Tyr
85 90 95
Ile Leu Ile Thr Gln Phe Pro Ile Pro Arg Thr Val Leu Thr Leu Ala
100 105 110
Ser Gly Val Leu Phe Gly Pro Val Leu Gly Ser Val Val Ala Leu Gly
115 120 125
Ser Thr Thr Val Ser Ala Val Ile Ser Leu Leu Ile Val Arg Gly Leu
130 135 140
Leu Gly Asp Trp Met Ala Pro Arg Leu Thr His Pro Ala Val Ser Arg
145 150 155 160
Ile Asn Thr Arg Leu Glu Gln Arg Gly Trp Leu Ala Ile Thr Ser Leu
165 170 175
Arg Met Ile Ala Ala Ile Pro Phe Ser Ile Leu Asn Tyr Val Ala Ala
180 185 190
Leu Thr Ser Val Pro Val Phe Ser phe Ala Ile Ala Thr Leu Ile Gly
195 200 205
Ser Ala Pro Gly Thr Ile Val Thr Val Val Leu Gly Asp Ala Val Thr
210 215 220
Gly Ser Gly Asn Trp Thr Ala Val Ala Phe Thr Val Phe Leu Ala Ile
225 230 235 240
Leu Gly Val Leu Gly Ile Phe Leu Asp Gln Lys Met Pro Val Lys Pro
245 250 255
Gly Lys
<210>23
<211>727
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(697)
<223>RXA00151
<400>23
aagtagacaa tataaggtag ggttggagaa gattctttac ttcttcactc attcgccctg 60
acagcgaatc cactacaata aacaaagggg gcagccaccc atg tgg gca ctg cat 115
Met Trp Ala Leu His
1 5
gcc aga tac cgc ggc cga gac aca cga cga gcc gaa ttg gtt aaa aga 163
Ala Arg Tyr Arg Gly Arg Asp Thr Arg Arg Ala Glu Leu Val Lys Arg
10 15 20
ttt gca gaa gct ttg tcc acc ctc gaa ggt gct ggc cag ttt gaa gtc 211
Phe Ala Glu Ala Leu Ser Thr Leu Glu Gly Ala Gly Gln Phe Glu Val
25 30 35
att ggt gtc gaa gac atc cga gca cac atc acc tca ccg tta acc aca 259
Ile Gly Val Glu Asp Ile Arg Ala His Ile Thr Ser Pro Leu Thr Thr
40 45 50
tgt gat gtt gtc atg gcg cta ctc gca gct ggc gat tgg gca atc gga 307
Cys Asp Val Val Met Ala Leu Leu Ala Ala Gly Asp Trp Ala Ile Gly
55 60 65
att ggt gtt atc ccc acc gtt gac gga aca gtc gat gaa tcg gat gag 355
Ile Gly Val Ile Pro Thr Val Asp Gly Thr Val Asp Glu Ser Asp Glu
70 75 80 85
gcc atc atc gaa cag gta aag aaa att tcc tct gat gct ctg cgc ccc 403
Ala Ile Ile Glu Gln Val Lys Lys Ile Ser Ser Asp Ala Leu Arg Pro
90 95 100
acc gcc aag gcg gga acg gtc aaa gtt agg atc gcc gga acc aaa cgg 451
Thr Ala Lys Ala Gly Thr Val Lys Val Arg Ile Ala Gly Thr Lys Arg
105 110 115
gac aat act caa gcc ttc aac atc tcc gct gca ttc acg ttg att ggg 499
Asp Asn Thr Gln Ala Phe Asn Ile Ser Ala Ala Phe Thr Leu Ile Gly
120 125 130
caa gtt ctg tca aag cgc acc atc gaa gga cgc gaa gcc acc gca ctc 547
Gln Val Leu Ser Lys Arg Thr Ile Glu Gly Arg Glu Ala Thr Ala Leu
135 140 145
gtc cgt tct gga ctg aat caa aat gag gca gcc caa gag ctc ggt att 595
Val Arg Ser Gly Leu Asn Gln Asn Glu Ala Ala Gln Glu Leu Gly Ile
150 155 160 165
tcc aag cag gca atg tcg cag cga ttg cag gcg gca ggt tgg cag gcg 643
Ser Lys Gln Ala Met Ser Gln Arg Leu Gln Ala Ala Gly Trp Gln Ala
170 175 180
gaa tct gct ggt tgg caa ttg gcc gta aac ctc atc gag cag gct ggc 691
Glu Ser Ala Gly Trp Gln Leu Ala Val Asn Leu Ile Glu Gln Ala Gly
185 190 195
aag cgc tagcaggcga tttaagagcc tgagtttgtg 727
Lys Arg
<210>24
<211>199
<212>PRT
<213>谷氨酸棒状杆菌
<400>24
Met Trp Ala Leu His Ala Arg Tyr Arg Gly Arg Asp Thr Arg Arg Ala
1 5 10 15
Glu Leu Val Lys Arg Phe Ala Glu Ala Leu Ser Thr Leu Glu Gly Ala
20 25 30
Gly Gln Phe Glu Val Ile Gly Val Glu Asp Ile Arg Ala His Ile Thr
35 40 45
Ser Pro Leu Thr Thr Cys Asp Val Val Met Ala Leu Leu Ala Ala Gly
50 55 60
Asp Trp Ala Ile Gly Ile Gly Val Ile Pro Thr Val Asp Gly Thr Val
65 70 75 80
Asp Glu Ser Asp Glu Ala Ile Ile Glu Gln Val Lys Lys Ile Ser Ser
85 90 95
Asp Ala Leu Arg Pro Thr Ala Lys Ala Gly Thr Val Lys Val Arg Ile
100 105 110
Ala Gly Thr Lys Arg Asp Asn Thr Gln Ala Phe Asn Ile Ser Ala Ala
115 120 125
Phe Thr Leu Ile Gly Gln Val Leu Ser Lys Arg Thr Ile Glu Gly Arg
130 135 140
Glu Ala Thr Ala Leu Val Arg Ser Gly Leu Asn Gln Asn Glu Ala Ala
145 150 155 160
Gln Glu Leu Gly Ile Ser Lys Gln Ala Met Ser Gln Arg Leu Gln Ala
165 170 175
Ala Gly Trp Gln Ala Glu Ser Ala Gly Trp Gln Leu Ala Val Asn Leu
180 185 190
Ile Glu Gln Ala Gly Lys Arg
195
<210>25
<211>913
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(883)
<223>RXA00155
<400>25
gttgcgggag tgtccaccaa aaatattgcc tgcatagggg tctgaaaaac tcatagctca 60
ccaccctagt caaagcactg cactacactt ccctaacact atg gaa att cga gca 115
Met Glu Ile Arg Ala
1 5
aac gtt tat tca ccg ctt caa aat act gcc gtc tgg cta ggt gcg tgg 163
Asn Val Tyr Ser Pro Leu Gln Asn Thr Ala Val Trp Leu Gly Ala Trp
10 15 20
ctc tat gaa tta gtg ccc acc gaa gat gtc atc gat gcg ttc gtc gat 211
Leu Tyr Glu Leu Val Pro Thr Glu Asp Val Ile Asp Ala Phe Val Asp
25 30 35
ctc ggc ggc ccg cac acc ttc ggc gac ggc ggg ctg ctc gat atg ctg 259
Leu Gly Gly Pro His Thr Phe Gly Asp Gly Gly Leu Leu Asp Met Leu
40 45 50
cga act tta aaa gaa tta aca agc acg ctt atc gac gcc ccc ttc cac 307
Arg Thr Leu Lys Glu Leu Thr Ser Thr Leu Ile Asp Ala Pro Phe His
55 60 65
ggt ccg att tta act ctc gca ctc agc ggc cca ggc caa gtg ccg gcg 355
Gly Pro Ile Leu Thr Leu Ala Leu Ser Gly Pro Gly Gln Val Pro Ala
70 75 80 85
ttg ccg gca ggt tca cgc gct gcg gtc ttg gcc tcc gcc tcg aag gag 403
Leu Pro Ala Gly Ser Arg Ala Ala Val Leu Ala Ser Ala Ser Lys Glu
90 95 100
ggg gcg ttg gtg ctg ggc ggc gtc gat aag cag cat tct tgg gcg ctt 451
Gly Ala Leu Val Leu Gly Gly Val Asp Lys Gln His Ser Trp Ala Leu
105 110 115
att cct acg cgc ggt aag gat gcc act gag tgg aat ttg gtg gag gtg 499
Ile Pro Thr Arg Gly Lys Asp Ala Thr Glu Trp Asn Leu Val Glu Val
120 125 130
gag ggc ttt ttg ccg gcg att gcg act gtc tcg ccc ggt gag gcg gat 547
Glu Gly Phe Leu Pro Ala Ile Ala Thr Val Ser Pro Gly Glu Ala Asp
135 140 145
cag ctg ctc cgt cag gcg aca gat cag gcg gca aat atc att gag tta 595
Gln Leu Leu Arg Gln Ala Thr Asp Gln Ala Ala Asn Ile Ile Glu Leu
150 155 160 165
agt ggc tac gct tca ctt gcg ccg aaa tct ttg aag aat ccg cga cta 643
Ser Gly Tyr Ala Ser Leu Ala Pro Lys Ser Leu Lys Asn Pro Arg Leu
170 175 180
acc gtg ggc atg ctg tca gat ttt tac gac acc ccg ggg ctt ccc tat 691
Thr Val Gly Met Leu Ser Asp Phe Tyr Asp Thr Pro Gly Leu Pro Tyr
185 190 195
gca gtc cca gag agg tcg gcg aag ctt ttt gcg cgc gca gat cgc gtg 739
Ala Val Pro Glu Arg Ser Ala Lys Leu Phe Ala Arg Ala Asp Arg Val
200 205 210
gct gcc atc gca gag acc gtg cag gaa acc atc ggc gat cac agc ctg 787
Ala Ala Ile Ala Glu Thr Val Gln Glu Thr Ile Gly Asp His Ser Leu
215 220 225
gat cca cag ttg att ttc ctg tgg agc cat att cgg acc gcg cgc atg 835
Asp Pro Gln Leu Ile Phe Leu Trp Ser His Ile Arg Thr Ala Arg Met
230 235 240 245
gct ggg gtg agt tat gcc ctc gca gag ttt gcg agg gac tat cac tcc 883
Ala Gly Val Ser Tyr Ala Leu Ala Glu Phe Ala Arg Asp Tyr His Ser
250 255 260
tagttggcag ctggcgccgc tgagcgagcg 913
<210>26
<211>261
<212>PRT
<213>谷氨酸棒状杆菌
<400>26
Met Glu Ile Arg Ala Asn Val Tyr Ser Pro Leu Gln Asn Thr Ala Val
1 5 10 15
Trp Leu Gly Ala Trp Leu Tyr Glu Leu Val Pro Thr Glu Asp Val Ile
20 25 30
Asp Ala Phe Val Asp Leu Gly Gly Pro His Thr Phe Gly Asp Gly Gly
35 40 45
Leu Leu Asp Met Leu Arg Thr Leu Lys Glu Leu Thr Ser Thr Leu Ile
50 55 60
Asp Ala Pro Phe His Gly Pro Ile Leu Thr Leu Ala Leu Ser Gly Pro
65 70 75 80
Gly Gln Val Pro Ala Leu Pro Ala Gly Ser Arg Ala Ala Val Leu Ala
85 90 95
Ser Ala Ser Lys Glu Gly Ala Leu Val Leu Gly Gly Val Asp Lys Gln
100 105 110
His Ser Trp Ala Leu Ile Pro Thr Arg Gly Lys Asp Ala Thr Glu Trp
115 120 125
Asn Leu Val Glu Val Glu Gly Phe Leu Pro Ala Ile Ala Thr Val Ser
130 135 140
Pro Gly Glu Ala Asp Gln Leu Leu Arg Gln Ala Thr Asp Gln Ala Ala
145 150 155 160
Asn Ile Ile Glu Leu Ser Gly Tyr Ala Ser Leu Ala Pro Lys Ser Leu
165 170 175
Lys Asn Pro Arg Leu Thr Val Gly Met Leu Ser Asp Phe Tyr Asp Thr
180 185 190
Pro Gly Leu Pro Tyr Ala Val Pro Glu Arg Ser Ala Lys Leu Phe Ala
195 200 205
Arg Ala Asp Arg Val Ala Ala Ile Ala Glu Thr Val Gln Glu Thr Ile
210 215 220
Gly Asp His Ser Leu Asp Pro Gln Leu Ile Phe Leu Trp Ser His Ile
225 230 235 240
Arg Thr Ala Arg Met Ala Gly Val Ser Tyr Ala Leu Ala Glu Phe Ala
245 250 255
Arg Asp Tyr His Ser
260
<210>27
<211>2758
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2728)
<223>RXA00185
<400>27
attgcgagag ctcgctgact ctgtggtcaa tggtgcagcg ccggaagaag accgcgatgc 60
gctcaaccta ttgttccgac taagccggga ggtctaagac atg cgt att cat gaa 115
Met Arg Ile His Glu
1 5
atc atc att gac aat ttc cga gcc atc gaa cat cta gaa ctt cgt gac 163
Ile Ile Ile Asp Asn Phe Arg Ala Ile Glu His Leu Glu Leu Arg Asp
10 15 20
att cct gac cag ggc gtt atc gtg atc cac ggt gac aat gag caa ggc 211
Ile Pro Asp Gln Gly Val Ile Val Ile His Gly Asp Asn Glu Gln Gly
25 30 35
aaa tct tca atc ctt gaa gcc ata aaa aca gtt ctg aat agt aaa cac 259
Lys Ser Ser Ile Leu Glu Ala Ile Lys Thr Val Leu Asn Ser Lys His
40 45 50
aga acc acc agc aag acg atc aag gcc att cag cct gtt gac cgt gat 307
Arg Thr Thr Ser Lys Thr Ile Lys Ala Ile Gln Pro Val Asp Arg Asp
55 60 65
gtt ccc atc agc atc acc ctc gag gca acc gta ggc acg gtt cga ttc 355
Val Pro Ile Ser Ile Thr Leu Glu Ala Thr Val Gly Thr Val Arg Phe
70 75 80 85
cgc ata cac aaa cgc ttc cta aaa tcc aca gcc gca gaa ctg caa gtc 403
Arg Ile His Lys Arg Phe Leu Lys Ser Thr Ala Ala Glu Leu Gln Val
90 95 100
atc gag cca cgt cca tcc aac cac cgt gga cta gaa gcc gaa gct gca 451
Ile Glu Pro Arg Pro Ser Asn His Arg Gly Leu Glu Ala Glu Ala Ala
105 110 115
ctg gca gaa ata ctg gaa agc cat ttg gac acc tct ttg ctt gac gca 499
Leu Ala Glu Ile Leu Glu Ser His Leu Asp Thr Ser Leu Leu Asp Ala
120 125 130
ttg ttt atg aaa cag ggt gaa gta gaa gca ggt atc agc gct gta gga 547
Leu Phe Met Lys Gln Gly Glu Val Glu Ala Gly Ile Ser Ala Val Gly
135 140 145
att cct acc ctg acc agc gct ttg aac gct caa aat ggc aat acc gaa 595
Ile Pro Thr Leu Thr Ser Ala Leu Asn Ala Gln Asn Gly Asn Thr Glu
150 155 160 165
gat gcc acc gaa gac acc gca ctc atg gag gct gta gaa aag gaa tac 643
Asp Ala Thr Glu Asp Thr Ala Leu Met Glu Ala Val Glu Lys Glu Tyr
170 175 180
cta aag ttc tac acc aac tcc gga aag gcg aat acg agg ttc cta cag 691
Leu Lys Phe Tyr Thr Asn Ser Gly Lys Ala Asn Thr Arg Phe Leu Gln
185 190 195
ttt tcc aaa cag gtg gaa acc ctt cgt act gat ttg gat gag gct aac 739
Phe Ser Lys Gln Val Glu Thr Leu Arg Thr Asp Leu Asp Glu Ala Asn
200 205 210
gct gaa gta gca aag ctc tcc tcc cat gtt gac cga gtt aaa cga ttg 787
Ala Glu Val Ala Lys Leu Ser Ser His Val Asp Arg Val Lys Arg Leu
215 220 225
gaa att gat cgg gat cag gcc aca gca cag tta ccc aag gca gag gaa 835
Glu Ile Asp Arg Asp Gln Ala Thr Ala Gln Leu Pro Lys Ala Glu Glu
230 235 240 245
gaa ctc gca ggg agg aaa gca gaa ctg gag gaa gcc caa aag gtt aag 883
Glu Leu Ala Gly Arg Lys Ala Glu Leu Glu Glu Ala Gln Lys Val Lys
250 255 260
gct caa gcc acg gaa atc ctt gcg cag ttt tcc cgg gcg gaa gaa cag 931
Ala Gln Ala Thr Glu Ile Leu Ala Gln Phe Ser Arg Ala Glu Glu Gln
265 270 275
tta gag cag gca aca gga gct cag aaa cga cgt aaa gaa ctc cgc aat 979
Leu Glu Gln Ala Thr Gly Ala Gln Lys Arg Arg Lys Glu Leu Arg Asn
280 285 290
aag ctg gaa tta gca cag aca gaa gtc gaa aag gct gaa gca ggg caa 1027
Lys Leu Glu Leu Ala Gln Thr Glu Val Glu Lys Ala Glu Ala Gly Gln
295 300 305
gag aac ctg gcg caa gaa gct tcc cgt gaa gca gaa gaa ttc cta gcc 1075
Glu Asn Leu Ala Gln Glu Ala Ser Arg Glu Ala Glu Glu Phe Leu Ala
310 315 320 325
tta agt gaa aag ttc gag gca gcg cgc gca gct gaa act ctg gcc gtt 1123
Leu Ser Glu Lys Phe Glu Ala Ala Arg Ala Ala Glu Thr Leu Ala Val
330 335 340
gaa aag gtg aaa gca gca cgt caa tcg gtt gct gga atc aaa aac cgt 1171
Glu Lys Val Lys Ala Ala Arg Gln Ser Val Ala Gly Ile Lys Asn Arg
345 350 355
gac cgt aaa gaa cac ctc acg gtt atg ctt ggt gaa ctt gat cga att 1219
Asp Arg Lys Glu His Leu Thr Val Met Leu Gly Glu Leu Asp Arg Ile
360 365 370
ggg cag cgc ctc tac gaa ttg cgc agc gtc cag cac tca tcg gtg cgt 1267
Gly Gln Arg Leu Tyr Glu Leu Arg Ser Val Gln His Ser Ser Val Arg
375 380 385
gtg agc cag cgt gat att gat gct ctg caa aag gcg atc acc gag gtc 1315
Val Ser Gln Arg Asp Ile Asp Ala Leu Gln Lys Ala Ile Thr Glu Val
390 395 400 405
gat atc caa agg acg ctc gtt gaa gcg cag cag gga agt att acc ctc 1363
Asp Ile Gln Arg Thr Leu Val Glu Ala Gln Gln Gly Ser Ile Thr Leu
410 415 420
agt gct tcc aca ccc acc gac att caa cta ggt gat gac acc gtt tca 1411
Ser Ala Ser Thr Pro Thr Asp Ile Gln Leu Gly Asp Asp Thr Val Ser
425 430 435
gct acc gac gct gga acc aca gtt gcc ctc gac cga gaa ctc acc gtc 1459
Ala Thr Asp Ala Gly Thr Thr Val Ala Leu Asp Arg Glu Leu Thr Val
440 445 450
gtg gtc ggc gat gtc acc ttg gtg att aac cca gga aaa aca gca gcg 1507
Val Val Gly Asp Val Thr Leu Val Ile Asn Pro Gly Lys Thr Ala Ala
455 460 465
gaa agt cgc acc gat ttt gaa tcc gcc gag gca gca ttg gcg gag ctg 1555
Glu Ser Arg Thr Asp Phe Glu Ser Ala Glu Ala Ala Leu Ala Glu Leu
470 475 480 485
ctt gat caa ctc gac gtc tca gac ttg gac cag ctc agg gaa cgt ttc 1603
Leu Asp Gln Leu Asp Val Ser Asp Leu Asp Gln Leu Arg Glu Arg Phe
490 495 500
act gcc caa gag caa cga gac gcg gat att gca gaa ctg gtt cgc gaa 1651
Thr Ala Gln Glu Gln Arg Asp Ala Asp Ile Ala Glu Leu Val Arg Glu
505 510 515
caa cag cgc atg agc ggt ggg aca gaa aca aca gtt tta cgt gcc gag 1699
Gln Gln Arg Met Ser Gly Gly Thr Glu Thr Thr Val Leu Arg Ala Glu
520 525 530
cta gaa ggc ctg cac gtc cca gag gat cta gac cca tca att tct gtg 1747
Leu Glu Gly Leu His Val Pro Glu Asp Leu Asp Pro Ser Ile Ser Val
535 540 545
gat gat gcg caa act cag ttg aat gag gca gaa gag tcc aga gag ctc 1795
Asp Asp Ala Gln Thr Gln Leu Asn Glu Ala Glu Glu Ser Arg Glu Leu
550 555 560 565
gct gcg gag gcg cat aaa cac gca aat gca gca ctt gat gga ttg cgt 1843
Ala Ala Glu Ala His Lys His Ala Asn Ala Ala Leu Asp Gly Leu Arg
570 575 580
tcg cgt ccc gta gat aaa gct ctg aca gtt ttc aac gct cag ctc gca 1891
Ser Arg Pro Val Asp Lys Ala Leu Thr Val Phe Asn Ala Gln Leu Ala
585 590 595
gca ctg cag aga aac ctc tct tcc gca caa gtt gaa tta gac cgt gca 1939
Ala Leu Gln Arg Asn Leu Ser Ser Ala Gln Val Glu Leu Asp Arg Ala
600 605 610
gtg gca gaa acc tcg gat gac gaa gtt gat gct gct gta cag cgg tgc 1987
Val Ala Glu Thr Ser Asp Asp Glu Val Asp Ala Ala Val Gln Arg Cys
615 620 625
gct gaa gct cta gct ggg gtg cgg gta cag aag cag gag att gaa cag 2035
Ala Glu Ala Leu Ala Gly Val Arg Val Gln Lys Gln Glu Ile Glu Gln
630 635 640 645
gtc ttg gca aaa acc aac cca gac atg gct caa cgc tta tgt gat gct 2083
Val Leu Ala Lys Thr Asn Pro Asp Met Ala Gln Arg Leu Cys Asp Ala
650 655 660
gct gaa gca aat gtg cga tcg tat aaa acg gca gtg tct gat gcc acc 2131
Ala Glu Ala Asn Val Arg Ser Tyr Lys Thr Ala Val Ser Asp Ala Thr
665 670 675
aca gag ttg gtg cgc ctg gaa ggc ctc atc ggt gta gct gca ggt gcg 2179
Thr Glu Leu Val Arg Leu Glu Gly Leu Ile Gly Val Ala Ala Gly Ala
680 685 690
aaa gas cga cta gat aaa gtt aag tct gcg ctg aca gca gct gaa aac 2227
Lys Glu Arg Leu Asp Lys Val Lys Ser Ala Leu Thr Ala Ala Glu Asn
695 700 705
cgg ttt gaa tca gaa caa cgt cgc gcc cac gct gca cgt cgc ctc tat 2275
Arg Phe Glu Ser Glu Gln Arg Arg Ala His Ala Ala Arg Arg Leu Tyr
710 715 720 725
gcc ctc atg gtg ttc tac cgt gat gaa tcc cga aag cgc tac gca gca 2323
Ala Leu Met Val Phe Tyr Arg Asp Glu Ser Arg Lys Arg Tyr Ala Ala
730 735 740
ccc ttc gcg gac aaa cta tcc agg ctg gca gca tcc gta ttt gga gaa 2371
Pro Phe Ala Asp Lys Leu Ser Arg Leu Ala Ala Ser Val Phe Gly Glu
745 750 755
tca gca gac ttt gat ctt gat gat gag ctg aag atc tcc agt cgt tcc 2419
Ser Ala Asp Phe Asp Leu Asp Asp Glu Leu Lys Ile Ser Ser Arg Ser
760 765 770
ata gga cca cga aca gtg gac ctt gcc aat ctt tct ggc ggc gca aag 2467
Ile Gly Pro Arg Thr Val Asp Leu Ala Asn Leu Ser Gly Gly Ala Lys
775 780 785
gaa cag ctg gca atc ctc acc cgc ttt gct atc gca gaa ttg gta gca 2515
Glu Gln Leu Ala Ile Leu Thr Arg Phe Ala Ile Ala Glu Leu Val Ala
790 795 800 805
gaa tcc tcc gcg caa ggt gct gtg cct gtg ttt att gat gat gca ttg 2563
Glu Ser Ser Ala Gln Gly Ala Val Pro Val Phe Ile Asp Asp Ala Leu
810 815 820
ggg agc aca gac cca gag cga ctg acc cgc ata tct act ctg ttt agt 2611
Gly Ser Thr Asp Pro Glu Arg Leu Thr Arg Ile Ser Thr Leu Phe Ser
825 830 835
gat gca ggc aag gac tct cag gtg ttt gtt ctc acc tgt gtt cca gac 2659
Asp Ala Gly Lys Asp Ser Gln Val Phe Val Leu Thr Cys Val Pro Asp
840 845 850
cgg tac aac tat gta gag gtc acc caa aag cac agc atc gag tca ttg 2707
Arg Tyr Asn Tyr Val Glu Val Thr Gln Lys His Ser Ile Glu Ser Leu
855 860 865
aaa act gcc aac gct ctg ctg tagctgagca aaaagccaag caagtagctg 2758
Lys Thr Ala Asn Ala Leu Leu
870 875
<210>28
<211>876
<212>PRT
<213>谷氨酸棒状杆菌
<400>28
Met Arg Ile His Glu Ile Ile Ile Asp Asn Phe Arg Ala Ile Glu His
1 5 10 15
Leu Glu Leu Arg Asp Ile Pro Asp Gln Gly Val Ile Val Ile His Gly
20 25 30
Asp Asn Glu Gln Gly Lys Ser Ser Ile Leu Glu Ala Ile Lys Thr Val
35 40 45
Leu Asn Ser Lys His Arg Thr Thr Ser Lys Thr Ile Lys Ala Ile Gln
50 55 60
Pro Val Asp Arg Asp Val Pro Ile Ser Ile Thr Leu Glu Ala Thr Val
65 70 75 80
Gly Thr Val Arg Phe Arg Ile His Lys Arg Phe Leu Lys Ser Thr Ala
85 90 95
Ala Glu Leu Gln Val Ile Glu Pro Arg Pro Ser Asn His Arg Gly Leu
100 105 110
Glu Ala Glu Ala Ala Leu Ala Glu Ile Leu Glu Ser His Leu Asp Thr
115 120 125
Ser Leu Leu Asp Ala Leu Phe Met Lys Gln Gly Glu Val Glu Ala Gly
130 135 140
Ile Ser Ala Val Gly Ile Pro Thr Leu Thr Ser Ala Leu Asn Ala Gln
145 150 155 160
Asn Gly Asn Thr Glu Asp Ala Thr Glu Asp Thr Ala Leu Met Glu Ala
165 170 175
Val Glu Lys Glu Tyr Leu Lys Phe Tyr Thr Asn Ser Gly Lys Ala Asn
180 185 190
Thr Arg Phe Leu Gln Phe Ser Lys Gln Val Glu Thr Leu Arg Thr Asp
195 200 205
Leu Asp Glu Ala Asn Ala Glu Val Ala Lys Leu Ser Ser His Val Asp
210 215 220
Arg Val Lys Arg Leu Glu Ile Asp Arg Asp Gln Ala Thr Ala Gln Leu
225 230 235 240
Pro Lys Ala Glu Glu Glu Leu Ala Gly Arg Lys Ala Glu Leu Glu Glu
245 250 255
Ala Gln Lys Val Lys Ala Gln Ala Thr Glu Ile Leu Ala Gln Phe Ser
260 265 270
Arg Ala Glu Glu Gln Leu Glu Gln Ala Thr Gly Ala Gln Lys Arg Arg
275 280 285
Lys Glu Leu Arg Asn Lys Leu Glu Leu Ala Gln Thr Glu Val Glu Lys
290 295 300
Ala Glu Ala Gly Gln Glu Asn Leu Ala Gln Glu Ala Ser Arg Glu Ala
305 310 315 320
Glu Glu Phe Leu Ala Leu Ser Glu Lys Phe Glu Ala Ala Arg Ala Ala
325 330 335
Glu Thr Leu Ala Val Glu Lys Val Lys Ala Ala Arg Gln Ser Val Ala
340 345 350
Gly Ile Lys Asn Arg Asp Arg Lys Glu His Leu Thr Val Met Leu Gly
355 360 365
Glu Leu Asp Arg Ile Gly Gln Arg Leu Tyr Glu Leu Arg Ser Val Gln
370 375 380
His Ser Ser Val Arg Val Ser Gln Arg Asp Ile Asp Ala Leu Gln Lys
385 390 395 400
Ala Ile Thr Glu Val Asp Ile Gln Arg Thr Leu Val Glu Ala Gln Gln
405 410 415
Gly Ser Ile Thr Leu Ser Ala Ser Thr Pro Thr Asp Ile Gln Leu Gly
420 425 430
Asp Asp Thr Val Ser Ala Thr Asp Ala Gly Thr Thr Val Ala Leu Asp
435 440 445
Arg Glu Leu Thr Val Val Val Gly Asp Val Thr Leu Val Ile Asn Pro
450 455 460
Gly Lys Thr Ala Ala Glu Ser Arg Thr Asp Phe Glu Ser Ala Glu Ala
465 470 475 480
Ala Leu Ala Glu Leu Leu Asp Gln Leu Asp Val Ser Asp Leu Asp Gln
485 490 495
Leu Arg Glu Arg Phe Thr Ala Gln Glu Gln Arg Asp Ala Asp Ile Ala
500 505 510
Glu Leu Val Arg Glu Gln Gln Arg Met Ser Gly Gly Thr Glu Thr Thr
515 520 525
Val Leu Arg Ala Glu Leu Glu Gly Leu His Val Pro Glu Asp Leu Asp
530 535 540
Pro Ser Ile Ser Val Asp Asp Ala Gln Thr Gln Leu Asn Glu Ala Glu
545 550 555 560
Glu Ser Arg Glu Leu Ala Ala Glu Ala His Lys His Ala Asn Ala Ala
565 570 575
Leu Asp Gly Leu Arg Ser Arg Pro Val Asp Lys Ala Leu Thr Val Phe
580 585 590
Asn Ala Gln Leu Ala Ala Leu Gln Arg Asn Leu Ser Ser Ala Gln Val
595 600 605
Glu Leu Asp Arg Ala Val Ala Glu Thr Ser Asp Asp Glu Val Asp Ala
610 615 620
Ala Val Gln Arg Cys Ala Glu Ala Leu Ala Gly Val Arg Val Gln Lys
625 630 635 640
Gln Glu Ile Glu Gln Val Leu Ala Lys Thr Asn Pro Asp Met Ala Gln
645 650 655
Arg Leu Cys Asp Ala Ala Glu Ala Asn Val Arg Ser Tyr Lys Thr Ala
660 665 670
Val Ser Asp Ala Thr Thr Glu Leu Val Arg Leu Glu Gly Leu Ile Gly
675 680 685
Val Ala Ala Gly Ala Lys Glu Arg Leu Asp Lys Val Lys Ser Ala Leu
690 695 700
Thr Ala Ala Glu Asn Arg Phe Glu Ser Glu Gln Arg Arg Ala His Ala
705 710 715 720
Ala Arg Arg Leu Tyr Ala Leu Met Val Phe Tyr Arg Asp G1u Ser Arg
725 730 735
Lys Arg Tyr Ala Ala Pro Phe Ala Asp Lys Leu Ser Arg Leu Ala Ala
740 745 750
Ser Val Phe Gly Glu Ser Ala Asp Phe Asp Leu Asp Asp Glu Leu Lys
755 760 765
Ile Ser Ser Arg Ser Ile Gly Pro Arg Thr Val Asp Leu Ala Asn Leu
770 775 780
Ser Gly Gly Ala Lys Glu Gln Leu Ala Ile Leu Thr Arg Phe Ala Ile
785 790 795 800
Ala Glu Leu Val Ala Glu Ser Ser Ala Gln Gly Ala Val Pro Val Phe
805 810 815
Ile Asp Asp Ala Leu Gly Ser Thr Asp Pro Glu Arg Leu Thr Arg Ile
820 825 830
Ser Thr Leu Phe Ser Asp Ala Gly Lys Asp Ser Gln Val Phe Val Leu
835 840 845
Thr Cys Val Pro Asp Arg Tyr Asr Tyr Val Glu Val Thr Gln Lys His
850 855 860
Ser Ile Glu Ser Leu Lys Thr Ala Asn Ala Leu Leu
865 870 875
<210>29
<211>1342
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1312)
<223>RXA00197
<400>29
gtcgatgata ttttggcaac cgaatctgag gcacgcgcgc gtgcgaatgc tttgatcaac 60
cggttggcaa ccaacttgta agctaaggag cttccgcctc gtg gca gcc tat ctt 115
Val Ala Ala Tyr Leu
1 5
ctt ggt gtc gta tta ttt ttc ctc ggc atc gca gta acc atc gcg ctt 163
Leu Gly Val Val Leu Phe Phe Leu Gly Ile Ala Val Thr Ile Ala Leu
10 15 20
cac gag tgg ggg cac ttc atc aca gcg cgc att ttc gga atg aaa gtg 211
His Glu Trp Gly His Phe Ile Thr Ala Arg Ile Phe Gly Met Lys Val
25 30 35
cgg cgt ttc ttc atc ggt ttc ggc ccg acg gtg ttt gcc aaa aga cgc 259
Arg Arg Phe Phe Ile Gly Phe Gly Pro Thr Val Phe Ala Lys Arg Arg
40 45 50
ggc gaa acc gtg tac ggc ctt aaa gcg att ccg gtc ggc ggt ttt tgt 307
Gly Glu Thr Val Tyr Gly Leu Lys Ala Ile Pro Val Gly Gly Phe Cys
55 60 65
gac atc gcg ggg atg act gcc caa gat gaa ctt gat ccg gaa gac ctg 355
Asp Ile Ala Gly Met Thr Ala Gln Asp Glu Leu Asp Pro Glu Asp Leu
70 75 80 85
ccg cgc gcc atg tat cta aag ccc tgg tgg cag cgc ata att gtg ctt 403
Pro Arg Ala Met Tyr Leu Lys Pro Trp Trp Gln Arg Ile Ile Val Leu
90 95 100
tcc ggc ggc gtg atc atg aat ctg atc gtc ggc ttt ttg gtg ctt tac 451
Ser Gly Gly Val Ile Met Asn Leu Ile Val Gly Phe Leu Val Leu Tyr
105 110 115
ggc gtg gcg gtg agc tcc gga atc ccg aat ccg gat gtg gat acc acc 499
Gly Val Ala Val Ser Ser Gly Ile Pro Asn Pro Asp Val Asp Thr Thr
120 125 130
gcg aca gtc gac acc gtt cag tgc gtg ccg gaa acc caa att tcc gca 547
Ala Thr Val Asp Thr Val Gln Cys Val Pro Glu Thr Gln Ile Ser Ala
135 140 145
act gaa ctg tcc tcc tgc gta ggt tca ggc cca gcg ggc gac gcc ggc 595
Thr Glu Leu Ser Ser Cys Val Gly Ser Gly Pro Ala Gly Asp Ala Gly
150 155 160 165
att gag cac ggc gat aag att ttg gcc gtc aac ggc caa gag atg gca 643
Ile Glu His Gly Asp Lys Ile Leu Ala Val Asn Gly Gln Glu Met Ala
170 175 180
agc ttc acc gcc atc cgc gat gcg atc ctc gag ctc cca ggc gaa acg 691
Ser Phe Thr Ala Ile Arg Asp Ala Ile Leu Glu Leu Pro Gly Glu Thr
185 190 195
gca acg ctg acg att gaa cgg gag gga acg ctt ttc gac gtc gac ctc 739
Ala Thr Leu Thr Ile Glu Arg Glu Gly Thr Leu Phe Asp Val Asp Leu
200 205 210
cag gtt gcc tct gtc acc cgt ctc gcc tct gac ggt tca gaa att acc 787
Gln Val Ala Ser Val Thr Arg Leu Ala Ser Asp Gly Ser Glu Ile Thr
215 220 225
gtc ggc gcg gtg ggc atg tcg agc ctt cca ccg acc gat gtg tac aaa 835
Val Gly Ala Val Gly Met Ser Ser Leu Pro Pro Thr Asp Val Tyr Lys
230 235 240 245
aaa tac ggc cca atc gag ggt gtg gga gca act gca cgt ttc acc ggc 883
Lys Tyr Gly Pro Ile Glu Gly Val Gly Ala Thr Ala Arg Phe Thr Gly
250 255 260
gac atg atc agc gcc acg tgg gat ggc ctc aaa gcc ttc ccg gcg aaa 931
Asp Met Ile Ser Ala Thr Trp Asp Gly Leu Lys Ala Phe Pro Ala Lys
265 270 275
atc cca ggg gtc gtc gca tcc atc ttc ggt gca gaa cga gat gta gaa 979
Ile Pro Gly Val Val Ala Ser Ile Phe Gly Ala Glu Arg Asp Val Glu
280 285 290
agc ccc atg agt gtg gtg ggc gcg tca cgc atc ggc ggc gaa ttt gtc 1027
Ser Pro Met Ser Val Val Gly Ala Ser Arg Ile Gly Gly Glu Phe Val
295 300 305
gaa cgt tcc atg tgg gac atg ttc atg atg atg ctg gcc agc ctg aac 1075
Glu Arg Ser Met Trp Asp Met Phe Met Met Met Leu Ala Ser Leu Asn
310 315 320 325
ttc ttc ctc gcg ctg ttt aac ctc gtg ccg ctg cca cca ctt gat ggc 1123
Phe Phe Leu Ala Leu Phe Asn Leu Val Pro Leu Pro Pro Leu Asp Gly
330 335 340
gga cac att gcc gtg gtg atc tat gaa aaa atc cgc gac ttc ttc cgc 1171
Gly His Ile Ala Val Val Ile Tyr Glu Lys Ile Arg Asp Phe Phe Arg
345 350 355
aaa ctg cgc gga aaa cca gcg ggc ggc cca gcg gat tac acc aaa cta 1219
Lys Leu Arg Gly Lys Pro Ala Gly Gly Pro Ala Asp Tyr Thr Lys Leu
360 365 370
atg ccc gtc acc gta gct gtc gca gcc ttg ctg atg aca gtg gga ggc 1267
Met Pro Val Thr Val Ala Val Ala Ala Leu Leu Met Thr Val Gly Gly
375 380 385
ctg gtc att gtc gcc gat gtg gtc aat ccc atc cga ctc ttt ggc 1312
Leu Val Ile Val Ala Asp Val Val Asn Pro Ile Arg Leu Phe Gly
390 395 400
taacgatacg gaattgaact gccggcaccg 1342
<210>30
<211>404
<212>PRT
<213>谷氨酸棒状杆菌
<400>30
Val Ala Ala Tyr Leu Leu Gly Val Val Leu Phe Phe Leu Gly Ile Ala
1 5 10 15
Val Thr Ile Ala Leu His Glu Trp Gly His Phe Ile Thr Ala Arg Ile
20 25 30
Phe Gly Met Lys Val Arg Arg Phe Phe Ile Gly Phe Gly Pro Thr Val
35 40 45
Phe Ala Lys Arg Arg Gly Glu Thr Val Tyr Gly Leu Lys Ala Ile Pro
50 55 60
Val Gly Gly Phe Cys Asp Ile Ala Gly Met Thr Ala Gln Asp Glu Leu
65 70 75 80
Asp Pro Glu Asp Leu Pro Arg Ala Met Tyr Leu Lys Pro Trp Trp Gln
85 90 95
Arg Ile Ile Val Leu Ser Gly Gly Val Ile Met Asn Leu Ile Val Gly
100 105 110
Phe Leu Val Leu Tyr Gly Val Ala Val Ser Ser Gly Ile Pro Asn Pro
115 120 125
Asp Val Asp Thr Thr Ala Thr Val Asp Thr Val Gln Cys Val Pro Glu
130 135 140
Thr Gln Ile Ser Ala Thr Glu Leu Ser Ser Cys Val Gly Ser Gly Pro
145 150 155 160
Ala Gly Asp Ala Gly Ile Glu His Gly Asp Lys Ile Leu Ala Val Asn
165 170 175
Gly Gln Glu Met Ala Ser Phe Thr Ala Ile Arg Asp Ala Ile Leu Glu
180 185 190
Leu Pro Gly Glu Thr Ala Thr Leu Thr Ile Glu Arg Glu Gly Thr Leu
195 200 205
Phe Asp Val Asp Leu Gln Val Ala Ser Val Thr Arg Leu Ala Ser Asp
210 215 220
Gly Ser Glu Ile Thr Val Gly Ala Val Gly Met Ser Ser Leu Pro Pro
225 230 235 240
Thr Asp Val Tyr Lys Lys Tyr Gly Pro Ile Glu Gly Val Gly Ala Thr
245 250 255
Ala Arg Phe Thr Gly Asp Met Ile Ser Ala Thr Trp Asp Gly Leu Lys
260 265 270
Ala Phe Pro Ala Lys Ile Pro Gly Val Val Ala Ser Ile Phe Gly Ala
275 280 285
Glu Arg Asp Val Glu Ser Pro Met Ser Val Val Gly Ala Ser Arg Ile
290 295 300
Gly Gly Glu Phe Val Glu Arg Ser Met Trp Asp Met Phe Met Met Met
305 310 315 320
Leu Ala Ser Leu Asn Phe Phe Leu Ala Leu Phe Asn Leu Val Pro Leu
325 330 335
Pro Pro Leu Asp Gly Gly His Ile Ala Val Val Ile Tyr Glu Lys Ile
340 345 350
Arg Asp Phe Phe Arg Lys Leu Arg Gly Lys Pro Ala Gly Gly Pro Ala
355 360 365
Asp Tyr Thr Lys Leu Met Pro Val Thr Val Ala Val Ala Ala Leu Leu
370 375 380
Met Thr Val Gly Gly Leu Val Ile Val Ala Asp Val Val Asn Pro Ile
385 390 395 400
Arg Leu Phe Gly
<210>31
<211>1615
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1585)
<223>RXA00199
<400>31
agtcagatcg cgcactcaat tagtcgcccg ggtttatcgc gcggaaattt cctgattagt 60
atccttaccc agcaccattt tttgtggttt gctctaagtt atg ggc gaa tca gta 115
Met Gly Glu Ser Val
1 5
agt act gac agg tac act ccg cag cag cgc acg cgt tat cgc cag ctg 163
Ser Thr Asp Arg Tyr Thr Pro Gln Gln Arg Thr Arg Tyr Arg Gln Leu
10 15 20
ctc atg gaa gat ctt gag att ttt gat cgg cat ttg cag cat tcc gat 211
Leu Met Glu Asp Leu Glu Ile Phe Asp Arg His Leu Gln His Ser Asp
25 30 35
ttt gag gat cag ggt tcc atc ggc ctc gag ttg gaa ctc aac ctc gtg 259
Phe Glu Asp Gln Gly Ser Ile Gly Leu Glu Leu Glu Leu Asn Leu Val
40 45 50
gat aag cat atg caa cct gct ttg gca ggc cac gcg gtg ctt tcc cat 307
Asp Lys His Met Gln Pro Ala Leu Ala Gly His Ala Val Leu Ser His
55 60 65
tta gac gat gag tac cag tcg gag atc ggt aac ttt aat gtt gaa atg 355
Leu Asp Asp Glu Tyr Gln Ser Glu Ile Gly Asn Phe Asn Val Glu Met
70 75 80 85
aac cac ccg cca cta tcc gtt aaa ggc gat gct tta cga cgc ctc gag 403
Asn His Pro Pro Leu Ser Val Lys Gly Asp Ala Leu Arg Arg Leu Glu
90 95 100
caa ggc atc acc tcg cgt cta ggt cgc gtg cgc gcg gcc gcc aca tcg 451
Gln Gly Ile Thr Ser Arg Leu Gly Arg Val Arg Ala Ala Ala Thr Ser
105 110 115
gag aac gtg aac gtg gcg atg atc ggc acg ctg ccc acc atc acc cca 499
Glu Ash Val Asn Val Ala Met Ile Gly Thr Leu Pro Thr Ile Thr Pro
120 125 130
gaa ttc ctc gaa gat ccg gca tgg atg acc caa gaa aac cgc tac cgt 547
Glu Phe Leu Glu Asp Pro Ala Trp Met Thr Gln Glu Asn Arg Tyr Arg
135 140 145
gcg ctg agc aat gcg gtg atg gaa tcg cgt ggc gag tta gtg cac atc 595
Ala Leu Ser Asn Ala Val Met Glu Ser Arg Gly Glu Leu Val His Ile
150 155 160 165
aac atc gcc gac cgt gaa caa atc atc cac gat ttc act gat ctc gcc 643
Asn Ile Ala Asp Arg Glu Gln Ile Ile His Asp Phe Thr Asp Leu Ala
170 175 180
ccc gaa tca act tgc acg tct att cag ttg cac ctg caa tta gca ccc 691
Pro Glu Ser Thr Cys Thr Ser Ile Gln Leu His Leu Gln Leu Ala Pro
185 190 195
aat aag ttt gct gca gcg tgg aat gcg tcg caa gca ata gct ggt gtt 739
Asn Lys Phe Ala Ala Ala Trp Asn Ala Ser Gln Ala Ile Ala Gly Val
200 205 210
caa gca gct ctt tcc gcc aac tcc cca ctt ttc cta ggc cgt cgg gtg 787
Gln Ala Ala Leu Ser Ala Asn Ser Pro Leu Phe Leu Gly Arg Arg Val
215 220 225
tgg cat gaa agc cgc att cca gtg ttt caa caa gct atc gat acg cgt 835
Trp His Glu Ser Arg Ile Pro Val Phe Gln Gln Ala Ile Asp Thr Arg
230 235 240 245
act cct gaa ctt gtg aat caa gga gtc cgc ccc aga gta tgg ttc ggc 883
Thr Pro Glu Leu Val Asn Gln Gly Val Arg Pro Arg Val Trp Phe Gly
250 255 260
gaa cga tgg atc acc agt gtt ttt gat ctt ttc gaa gaa aac gta cgc 931
Glu Arg Trp Ile Thr Ser Val Phe Asp Leu Phe Glu Glu Asn Val Arg
265 270 275
tac ttc tcc cct ctc atc gca gaa tcc cgc gcc ctg tct ggc aca cca 979
Tyr Phe Ser Pro Leu Ile Ala Glu Ser Arg Ala Leu Ser Gly Thr Pro
280 285 290
atg atg aag gga aaa tcc ccc gca ctc cac tac ctc aat ctc cac aac 1027
Met Met Lys Gly Lys Ser Pro Ala Leu His Tyr Leu Asn Leu His Asn
295 300 305
gga acg gtg tgg cgc tgg aac cga ccc att tac gca ccg ggg gaa gag 1075
Gly Thr Val Trp Arg Trp Asn Arg Pro Ile Tyr Ala Pro Gly Glu Glu
310 315 320 325
cgc tct cat ctg cgt ttg gaa aac cgt ctc cta cct gca ggt ccc acc 1123
Arg Ser His Leu Arg Leu Glu Asn Arg Leu Leu Pro Ala Gly Pro Thr
330 335 340
ccc atc gac atc acc gcc gat gcc gct ttc tac tac ggt ttg gtt aaa 1171
Pro Ile Asp Ile Thr Ala Asp Ala Ala Phe Tyr Tyr Gly Leu Val Lys
345 350 355
tat ctg gcc gag gaa aac cgc cct gtc tgg tca cgt ctc cta ttc cct 1219
Tyr Leu Ala Glu Glu Asn Arg Pro Val Trp Ser Arg Leu Leu Phe Pro
360 365 370
gat gcg gaa aag aac ttc caa tcc ggt gca cgt tca ggg ctg ttc gcc 1267
Asp Ala Glu Lys Asn Phe Gln Ser Gly Ala Arg Ser Gly Leu Phe Ala
375 380 385
cgc atg acc tgg cca acc ctc gga caa gtc aac gtt gcg aat ctg gtc 1315
Arg Met Thr Trp Pro Thr Leu Gly Gln Val Asn Val Ala Asn Leu Val
390 395 400 405
caa gag cac ctc atc cct caa gcc aga atc gga ctg gaa cga ctc gaa 1363
Gln Glu His Leu Ile Pro Gln Ala Arg Ile Gly Leu Glu Arg Leu Glu
410 415 420
gta aac aaa gac ctt atc gac caa tac ctg ggc atc atc acc gaa cgc 1411
Val Asn Lys Asp Leu Ile Asp Gln Tyr Leu Gly Ile Ile Thr Glu Arg
425 430 435
gcg aag tcc cgc caa aac ggt gcc acc tgg cag ttg cgt agc ctc aac 1459
Ala Lys Ser Arg Gln Asn Gly Ala Thr Trp Gln Leu Arg Ser Leu Asn
440 445 450
aag ctg gaa gca cac gga agc atg ccg ggt tcc gat gct cga aaa gca 1507
Lys Leu Glu Ala His Gly Ser Met Pro Gly Ser Asp Ala Arg Lys Ala
455 460 465
ggt ctt gca gca atg ttg caa cag tat tta cag aat caa gaa agc ggc 1555
Gly Leu Ala Ala Met Leu Gln Gln Tyr Leu Gln Asn Gln Glu Ser Gly
470 475 480 485
caa cct gtc cac aca tgg gct att ggt tcg taaaacggca aggaaagata 1605
Gln Pro Val His Thr Trp Ala Ile Gly Ser
490 495
atacccggaa 1615
<210>32
<211>495
<212>PRT
<213>谷氨酸棒状杆菌
<400>32
Met Gly Glu Ser Val Ser Thr Asp Arg Tyr Thr Pro Gln Gln Arg Thr
1 5 10 15
Arg Tyr Arg Gln Leu Leu Met Glu Asp Leu Glu Ile Phe Asp Arg His
20 25 30
Leu Gln His Ser Asp Phe Glu Asp Gln Gly Ser Ile Gly Leu Glu Leu
35 40 45
Glu Leu Asn Leu Val Asp Lys His Met Gln Pro Ala Leu Ala Gly His
50 55 60
Ala Val Leu Ser His Leu Asp Asp Glu Tyr Gln Ser Glu Ile Gly Asn
65 70 75 80
Phe Asn Val Glu Met Asn His Pro Pro Leu Ser Val Lys Gly Asp Ala
85 90 95
Leu Arg Arg Leu Glu Gln Gly Ile Thr Ser Arg Leu Gly Arg Val Arg
100 105 110
Ala Ala Ala Thr Ser Glu Asn Val Asn Val Ala Met Ile Gly Thr Leu
115 120 125
Pro Thr Ile Thr Pro Glu Phe Leu Glu Asp Pro Ala Trp Met Thr Gln
130 135 140
Glu Asn Arg Tyr Arg Ala Leu Ser Asn Ala Val Met Glu Ser Arg Gly
145 150 155 160
Glu Leu Val His Ile Asn Ile Ala Asp Arg Glu Gln Ile Ile His Asp
165 170 175
Phe Thr Asp Leu Ala Pro Glu Ser Thr Cys Thr Ser Ile Gln Leu His
180 185 190
Leu Gln Leu Ala Pro Asn Lys Phe Ala Ala Ala Trp Asn Ala Ser Gln
195 200 205
Ala Ile Ala Gly Val Gln Ala Ala Leu Ser Ala Asn Ser Pro Leu Phe
210 215 220
Leu Gly Arg Arg Val Trp His Glu Ser Arg Ile Pro Val Phe Gln Gln
225 230 235 240
Ala Ile Asp Thr Arg Thr Pro Glu Leu Val Asn Gln Gly Val Arg Pro
245 250 255
Arg Val Trp Phe Gly Glu Arg Trp Ile Thr Ser Val Phe Asp Leu Phe
260 265 270
Glu Glu Asn Val Arg Tyr Phe Ser Pro Leu Ile Ala Glu Ser Arg Ala
275 280 285
Leu Ser Gly Thr Pro Met Met Lys Gly Lys Ser Pro Ala Leu His Tyr
290 295 300
Leu Asn Leu His Asn Gly Thr Val Trp Arg Trp Asn Arg Pro Ile Tyr
305 310 315 320
Ala Pro Gly Glu Glu Arg Ser His Leu Arg Leu Glu Asn Arg Leu Leu
325 330 335
Pro Ala Gly Pro Thr Pro Ile Asp Ile Thr Ala Asp Ala Ala Phe Tyr
340 345 350
Tyr Gly Leu Val Lys Tyr Leu Ala Glu Glu Asn Arg Pro Val Trp Ser
355 360 365
Arg Leu Leu Phe Pro Asp Ala Glu Lys Asn Phe Gln Ser Gly Ala Arg
370 375 380
Ser Gly Leu Phe Ala Arg Met Thr Trp Pro Thr Leu Gly Gln Val Asn
385 390 395 400
Val Ala Asn Leu Val Gln Glu His Leu Ile Pro Gln Ala Arg Ile Gly
405 410 415
Leu Glu Arg Leu Glu Val Asn Lys Asp Leu Ile Asp Gln Tyr Leu Gly
420 425 430
Ile Ile Thr Glu Arg Ala Lys Ser Arg Gln Asn Gly Ala Thr Trp Gln
435 440 445
Leu Arg Ser Leu Asn Lys Leu Glu Ala His Gly Ser Met Pro Gly Ser
450 455 460
Asp Ala Arg Lys Ala Gly Leu Ala Ala Met Leu Gln Gln Tyr Leu Gln
465 470 475 480
Asn Gln Glu Ser Gly Gln Pro Val His Thr Trp Ala Ile Gly Ser
485 490 495
<210>33
<211>793
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(763)
<223>RXA00211
<400>33
tgagccaaaa tcaataaggt gtttttcagc ctgaggtaaa aatacggtgg tactgtcgaa 60
accaatcatc ccctagtttt gaaaagaagg aagcgagcca atg tca ttc ctg atc 115
Met Ser Phe Leu Ile
1 5
cgc gtc ctg ttg tcc gac acc cca ggc agc ctc gcg tta ctc gct gaa 163
Arg Val Leu Leu Ser Asp Thr Pro Gly Ser Leu Ala Leu Leu Ala Glu
10 15 20
gcc ctt ggg att gta gag gcc aat att caa tcc gtg gac gtg gtg gaa 211
Ala Leu Gly Ile Val Glu Ala Asn Ile Gln Ser Val Asp Val Val Glu
25 30 35
cgc ttc ccc aat ggc acg gtc atg gac gat ctg gtg atc tcc atc cct 259
Arg Phe Pro Asn Gly Thr Val Met Asp Asp Leu Val Ile Ser Ile Pro
40 45 50
cgc gat gtc atg gca gac acc atc atc acc gca gct gaa gaa gtc gac 307
Arg Asp Val Met Ala Asp Thr Ile Ile Thr Ala Ala Glu Glu Val Asp
55 60 65
ggc gtg gag att gat tcc atc cgc cca ttc tcc ggg act gtt gac cgc 355
Gly Val Glu Ile Asp Ser Ile Arg Pro Phe Ser Gly Thr Val Asp Arg
70 75 80 85
cgc gga cag atc caa atg ctg gct gct gtt gct cac caa cgc cgc gat 403
Arg Gly Gln Ile Gln Met Leu Ala Ala Val Ala His Gln Arg Arg Asp
90 95 100
atc acc gca gcg atg gaa gaa atg gtc gat gtc atc ccc cgc acc atg 451
Ile Thr Ala Ala Met Glu Glu Met Val Asp Val Ile Pro Arg Thr Met
105 110 115
acc tct ggt tgg gct ttg gtc att gat cta aaa gga ccc atc act cgc 499
Thr Ser Gly Trp Ala Leu Val Ile Asp Leu Lys Gly Pro Ile Thr Arg
120 125 130
atc gct ggt tcc cta gca gcg ccc gaa gat gac ggc acc gtt ccg gag 547
Ile Ala Gly Ser Leu Ala Ala Pro Glu Asp Asp Gly Thr Val Pro Glu
135 140 145
aac atc gtt ctc aaa gaa gct cgc atg ctc aac ccg gaa aac gat ccg 595
Asn Ile Val Leu Lys Glu Ala Arg Met Leu Asn Pro Glu Asn Asp Pro
150 155 160 165
tgg att cca gag tcc tgg aca ctg ctt gat tct tcc ctt gcc atc gct 643
Trp Ile Pro Glu Ser Trp Thr Leu Leu Asp Ser Ser Leu Ala Ile Ala
170 175 180
ccg atc ggc aag cac ggc ctg gct ctg att atc ggt cgc cct ggt ggc 691
Pro Ile Gly Lys His Gly Leu Ala Leu Ile Ile Gly Arg Pro Gly Gly
185 190 195
cct gat ttc ttg gcc agc gaa gtg gag cac tta ggc caa gtc ggt gac 739
Pro Asp Phe Leu Ala Ser Glu Val Glu His Leu Gly Gln Val Gly Asp
200 205 210
att atc gga gca atg ctt caa aaa taatctgagc tgtttaaaaa atgccccaag 793
Ile Ile Gly Ala Met Leu Gln Lys
215 220
<210>34
<211>221
<212>PRT
<213>谷氨酸棒状杆菌
<400>34
Met Ser Phe Leu Ile Arg Val Leu Leu Ser Asp Thr Pro Gly Ser Leu
1 5 10 15
Ala Leu Leu Ala Glu Ala Leu Gly Ile Val Glu Ala Asn Ile Gln Ser
20 25 30
Val Asp Val Val Glu Arg Phe Pro Asn Gly Thr Val Met Asp Asp Leu
35 40 45
Val Ile Ser Ile Pro Arg Asp Val Met Ala Asp Thr Ile Ile Thr Ala
50 55 60
Ala Glu Glu Val Asp Gly Val Glu Ile Asp Ser Ile Arg Pro Phe Ser
65 70 75 80
Gly Thr Val Asp Arg Arg Gly Gln Ile Gln Met Leu Ala Ala Val Ala
85 90 95
His Gln Arg Arg Asp Ile Thr Ala Ala Met Glu Glu Met Val Asp Val
100 105 110
Ile Pro Arg Thr Met Thr Ser Gly Trp Ala Leu Val Ile Asp Leu Lys
115 120 125
Gly Pro Ile Thr Arg Ile Ala Gly Ser Leu Ala Ala Pro Glu Asp Asp
130 135 140
Gly Thr Val Pro Glu Asn Ile Val Leu Lys Glu Ala Arg Met Leu Asn
145 150 155 160
Pro Glu Asn Asp Pro Trp Ile Pro Glu Ser Trp Thr Leu Leu Asp Ser
165 170 175
Ser Leu Ala Ile Ala Pro Ile Gly Lys His Gly Leu Ala Leu Ile Ile
180 185 190
Gly Arg Pro Gly Gly Pro Asp Phe Leu Ala Ser Glu Val Glu His Leu
195 200 205
Gly Gln Val Gly Asp Ile Ile Gly Ala Met Leu Gln Lys
210 215 220
<210>35
<211>634
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(604)
<223>RXA00220
<400>35
agatcgtgcc gagtttccgg tttggatggg gtttgggatg ttaatgggag aggatcggcg 60
tgatcgtgcc ggtttttctt gcgttggtag tcttggggct atg agc gca ttc cac 115
Met Ser Ala Phe His
1 5
atc cga gcg gaa caa tcg cgc gat atc agc gcg att cat gat gtt act 163
Ile Arg Ala Glu Gln Ser Arg Asp Ile Ser Ala Ile His Asp Val Thr
10 15 20
gaa gcg gcc ttt aca gga att gag cat tca gat gga aca gag caa gat 211
Glu Ala Ala Phe Thr Gly Ile Glu His Ser Asp Gly Thr Glu Gln Asp
25 30 35
ctc gtc gat aag ctt cgt gct gca aag gcg ctg agc ctt tcg ctc gtc 259
Leu Val Asp Lys Leu Arg Ala Ala Lys Ala Leu Ser Leu Ser Leu Val
40 45 50
gct gag gcg gat ggc gag gtc att ggg cat atc gca gcc tct gag gta 307
Ala Glu Ala Asp Gly Glu Val Ile Gly His Ile Ala Ala Ser Glu Val
55 60 65
ctg atc ggc ggt ggg gtg cag ggc tgg ttt ggc att ggg cct gtc agt 355
Leu Ile Gly Gly Gly Val Gln Gly Trp Phe Gly Ile Gly Pro Val Ser
70 75 80 85
gtt cgg ccc gat aag cag cag caa ggc gtg ggt att gcg ctg atg ggc 403
Val Arg Pro Asp Lys Gln Gln Gln Gly Val Gly Ile Ala Leu Met Gly
90 95 100
agc gcg ctt gat cag ttg cgt gcg gag ggt gct ggc ggc atc gtg ttg 451
Ser Ala Leu Asp Gln Leu Arg Ala Glu Gly Ala Gly Gly Ile Val Leu
105 110 115
ctg ggg gat ccg ggc tat tat cga cgc ttc ggt ttt gag gtc gtg ccc 499
Leu Gly Asp Pro Gly Tyr Tyr Arg Arg Phe Gly Phe Glu Val Val Pro
120 125 130
ggg ctg gtc tat ccg gac gcg cca gcg gaa ttt ttt atg gct gtg tgt 547
Gly Leu Val Tyr Pro Asp Ala Pro Ala Glu Phe Phe Met Ala Val Cys
135 140 145
ttg aat gct ccg gcg ttt ccg cag ggt gtt gtg gag tat cac tcg gca 595
Leu Asn Ala Pro Ala Phe Pro Gln Gly Val Val Glu Tyr His Ser Ala
150 155 160 165
ttt gga ggg tagagacccc atcgtggcgg gcctgggctc 634
Phe Gly Gly
<210>36
<211>168
<212>PRT
<213>谷氨酸棒状杆菌
<400>36
Met Ser Ala Phe His Ile Arg Ala Glu Gln Ser Arg Asp Ile Ser Ala
1 5 10 15
Ile His Asp Val Thr Glu Ala Ala Phe Thr Gly Ile Glu His Ser Asp
20 25 30
Gly Thr Glu Gln Asp Leu Val Asp Lys Leu Arg Ala Ala Lys Ala Leu
35 40 45
Ser Leu Ser Leu Val Ala Glu Ala Asp Gly Glu Val Ile Gly His Ile
50 55 60
Ala Ala Ser Glu Val Leu Ile Gly Gly Gly Val Gln Gly Trp Phe Gly
65 70 75 80
Ile Gly Pro Val Ser Val Arg Pro Asp Lys Gln Gln Gln Gly Val Gly
85 90 95
Ile Ala Leu Met Gly Ser Ala Leu Asp Gln Leu Arg Ala Glu Gly Ala
100 105 110
Gly Gly Ile Val Leu Leu Gly Asp Pro Gly Tyr Tyr Arg Arg Phe Gly
115 120 125
Phe Glu Val Val Pro Gly Leu Val Tyr Pro Asp Ala Pro Ala Glu Phe
130 135 140
Phe Met Ala Val Cys Leu Asn Ala Pro Ala Phe Pro Gln Gly Val Val
145 150 155 160
Glu Tyr His Ser Ala Phe Gly Gly
165
<210>37
<211>373
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<22l>CDS
<222>(101)..(343)
<223>RXA00240
<400>37
ttgagacccc gcagaatctg attgagtatt cactcattta acgtagttga cgtacggcga 60
agtcgctaaa cttcgcgtaa tcttggggcc aattaatcaa gtg cag atg aat tgt 115
Val Gln Met Asn Cys
1 5
tta gaa agg ctg gag acc atg ggt att ttt gac gaa gct aag aag aag 163
Leu Glu Arg Leu Glu Thr Met Gly Ile Phe Asp Glu Ala Lys Lys Lys
10 15 20
gca acc gaa ttc ctc gac tct gat tca ggc gag cag aag tcc gat gga 211
Ala Thr Glu Phe Leu Asp Ser Asp Ser Gly Glu Gln Lys Ser Asp Gly
25 30 35
ctg ctg gat aag gcc gca gac aaa gct aag ggt ctc ctc ggt gaa gac 259
Leu Leu Asp Lys Ala Ala Asp Lys Ala Lys Gly Leu Leu Gly Glu Asp
40 45 50
aaa gcg gat cag att aac aag gtc cgt gac gct gtc gat gag cgc att 307
Lys Ala Asp Gln Ile Asn Lys Val Arg Asp Ala Val Asp Glu Arg Ile
55 60 65
ggc aaa aac aac ggc ggc gaa gag aat cca gcc aac tagtattagg 353
Gly Lys Asn Asn Gly Gly Glu Glu Asn Pro Ala Asn
70 75 80
cgtcatgcgc gtcgtagttg 373
<210>38
<211>81
<212>PRT
<213>谷氨酸棒状杆菌
<400>38
Val Gln Met Asn Cys Leu Glu Arg Leu Glu Thr Met Gly Ile Phe Asp
1 5 10 15
Glu Ala Lys Lys Lys Ala Thr Glu Phe Leu Asp Ser Asp Ser Gly Glu
20 25 30
Gln Lys Ser Asp Gly Leu Leu Asp Lys Ala Ala Asp Lys Ala Lys Gly
35 40 45
Leu Leu Gly Glu Asp Lys Ala Asp Gln Ile Asn Lys Val Arg Asp Ala
50 55 60
Val Asp Glu Arg Ile Gly Lys Asn Asn Gly Gly Glu Glu Asn Pro Ala
65 70 75 80
Asn
<210>39
<21l>943
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(913)
<223>RXA00354
<400>39
ggcttgtcgg taaggctgca gggtagcggg agtttccttc agattggaag ttctttaatt 60
ttctcgatta tgtacctcat ttacgcgtaa agtttggggc atg gga aag tta ctt 115
Met Gly Lys Leu Leu
1 5
ttc gta gac atc ggt ggc aca ctg ctg gat tac tca aat gaa gtt ccg 163
Phe Val Asp Ile Gly Gly Thr Leu Leu Asp Tyr Ser Asn Glu Val Pro
10 15 20
cgt tcg gcc gtt gac gcg atc cgt aag gca cgc gcc aaa gga cac cgc 211
Arg Ser Ala Val Asp Ala Ile Arg Lys Ala Arg Ala Lys Gly His Arg
25 30 35
gtg tac ttg agc tct ggt cga agc agc gct gag gtg act tct cag ttg 259
Val Tyr Leu Ser Ser Gly Arg Ser Ser Ala Glu Val Thr Ser Gln Leu
40 45 50
tgg gat atc gga gtg gat ggc ctc att ggc gca aat ggt gga tat gtg 307
Trp Asp Ile Gly Val Asp Gly Leu Ile Gly Ala Asn Gly Gly Tyr Val
55 60 65
gaa agc gca cag gag tct gtg ttc cac cgc cgt ttg tcg ggt gag gag 355
Glu Ser Ala Gln Glu Ser Val Phe His Arg Arg Leu Ser Gly Glu Glu
70 75 80 85
acc cgc cac att gtg gag tgg ctc tac aac cgt ggt ttg gag ttt tat 403
Thr Arg His Ile Val Glu Trp Leu Tyr Asn Arg Gly Leu Glu Phe Tyr
90 95 100
ctc gag tcc aac aac ggt ttg tat gca agc cgt ggt ttc cgt gag gct 451
Leu Glu Ser Asn Asn Gly Leu Tyr Ala Ser Arg Gly Phe Arg Glu Ala
105 110 115
tct aag cca gtg ctg tct cgc ctt tcg gag aag acc gac gtg aca gtc 499
Ser Lys Pro Val Leu Ser Arg Leu Ser Glu Lys Thr Asp Val Thr Val
120 125 130
gat agc atg tac ccg gat atg ttc tgg ggc gcg agc ctt gat cgt gac 547
Asp Ser Met Tyr Pro Asp Met Phe Trp Gly Ala Ser Leu Asp Arg Asp
135 140 145
gat gtg aac aag atc agt tac atc ttc aat tct cag gaa gat ttg gac 595
Asp Val Asn Lys Ile Ser Tyr Ile Phe Asn Ser Gln Glu Asp Leu Asp
150 155 160 165
gca gcg cgt gag gcg ttc cct aac ctg gag cac acc acg tgg ggt ggt 643
Ala Ala Arg Glu Ala Phe Pro Asn Leu Glu His Thr Thr Trp Gly Gly
170 175 180
cag acg ggt gcg ttg ttc ggc acg atc ggt gtg tct gtc aac aag aag 691
Gln Thr Gly Ala Leu Phe Gly Thr Ile Gly Val Ser Val Asn Lys Lys
185 190 195
atc ggc gtg gat cgc ctg ctg aag tac ctg aac gca gat cgc gca aac 739
Ile Gly Val Asp Arg Leu Leu Lys Tyr Leu Asn Ala Asp Arg Ala Asn
200 205 210
acc att gcg ttc ggc gac agc gat gag gat ctc tcc cta ttt gag gcg 787
Thr Ile Ala Phe Gly Asp Ser Asp Glu Asp Leu Ser Leu Phe Glu Ala
215 220 225
agc gct tac ggc gtc gcg atg ggc gag gcc acc gaa tcg ctc aag gct 835
Ser Ala Tyr Gly Val Ala Met Gly Glu Ala Thr Glu Ser Leu Lys Ala
230 235 240 245
gct gct gac ctg gtc acg gat gct gtt ggg cag gac ggc ttg cgc aat 883
Ala Ala Asp Leu Val Thr Asp Ala Val Gly Gln Asp Gly Leu Arg Asn
250 255 260
gcg ttt tta aag ctt gag ctt atc gac gcc tgaccccatc aaagaacttc 933
Ala Phe Leu Lys Leu Glu Leu Ile Asp Ala
265 270
ccaatctcct 943
<210>40
<211>271
<212>PRT
<213>谷氨酸棒状杆菌
<400>40
Met Gly Lys Leu Leu Phe Val Asp Ile Gly Gly Thr Leu Leu Asp Tyr
1 5 10 15
Ser Asn Glu Val Pro Arg Ser Ala Val Asp Ala Ile Arg Lys Ala Arg
20 25 30
Ala Lys Gly His Arg Val Tyr Leu Ser Ser Gly Arg Ser Ser Ala Glu
35 40 45
Val Thr Ser Gln Leu Trp Asp Ile Gly Val Asp Gly Leu Ile Gly Ala
50 55 60
Asn Gly Gly Tyr Val Glu Ser Ala Gln Glu Ser Val Phe His Arg Arg
65 70 75 80
Leu Ser Gly Glu Glu Thr Arg His Ile Val Glu Trp Leu Tyr Asn Arg
85 90 95
Gly Leu Glu Phe Tyr Leu Glu Ser Asn Asn Gly Leu Tyr Ala Ser Arg
100 105 110
Gly Phe Arg Glu Ala Ser Lys Pro Val Leu Ser Arg Leu Ser Glu Lys
115 120 125
Thr Asp Val Thr Val Asp Ser Met Tyr Pro Asp Met Phe Trp Gly Ala
130 135 140
Ser Leu Asp Arg Asp Asp Val Asn Lys Ile Ser Tyr Ile Phe Asn Ser
145 150 155 160
Gln Glu Asp Leu Asp Ala Ala Arg Glu Ala Phe Pro Asn Leu Glu His
165 170 175
Thr Thr Trp Gly Gly Gln Thr Gly Ala Leu Phe Gly Thr Ile Gly Val
180 185 190
Ser Val Asn Lys Lys Ile Gly Val Asp Arg Leu Leu Lys Tyr Leu Asn
195 200 205
Ala Asp Arg Ala Asn Thr Ile Ala Phe Gly Asp Ser Asp Glu Asp Leu
210 215 220
Ser Leu Phe Glu Ala Ser Ala Tyr Gly Val Ala Met Gly Glu Ala Thr
225 230 235 240
Glu Ser Leu Lys Ala Ala Ala Asp Leu Val Thr Asp Ala Val Gly Gln
245 250 255
Asp Gly Leu Arg Asn Ala Phe Leu Lys Leu Glu Leu Ile Asp Ala
260 265 270
<210>41
<211>931
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(901)
<223>RXA00397
<400>41
tttttcgagg ttggggtggc ttttcgggtg gcgtgtatgt attaaaccaa ggagcttgtt 60
tgagggggag cgcttggttt gattcgacag ttttcgggca ttg agc ctg acg gaa 115
Leu Ser Leu Thr Glu
1 5
ggg gag atc att ccc atc caa aca aag ctc ggc cac tgg cgt gac gtg 163
Gly Glu Ile Ile Pro Ile Gln Thr Lys Leu Gly His Trp Arg Asp Val
10 15 20
cgg gat tcc gaa gat ctg cca ttc caa gta atc gca tcg cgc acc agc 211
Arg Asp Ser Glu Asp Leu Pro Phe Gln Val Ile Ala Ser Arg Thr Ser
25 30 35
gtg acc att atg gaa aac acc ctg gaa tgg acc aac agc cgc ctt gag 259
Val Thr Ile Met Glu Asn Thr Leu Glu Trp Thr Asn Ser Arg Leu Glu
40 45 50
cgt ggc gaa gag ttc acc gag ctc gac att tat gct cac tac ttg gaa 307
Arg Gly Glu Glu Phe Thr Glu Leu Asp Ile Tyr Ala His Tyr Leu Glu
55 60 65
gag ttg gaa gat tac gcc ctc caa ttc acc ttc gat gtc gag ccc tac 355
Glu Leu Glu Asp Tyr Ala Leu Gln Phe Thr Phe Asp Val Glu Pro Tyr
70 75 80 85
ttc acc aac ctg cac tcc tcc aac aga atg ctc ttc cct gga cct cct 403
Phe Thr Asn Leu His Ser Ser Asn Arg Met Leu Phe Pro Gly Pro Pro
90 95 100
gtg gat ttc ccg atc aac gca gaa acc cga tgc att cag ctc gac gcc 451
Val Asp Phe Pro Ile Asn Ala Glu Thr Arg Cys Ile Gln Leu Asp Ala
105 110 115
ggt gtt gca gta aag aag gac ggc gtg gtg ctg ggt acc tca gat atg 499
Gly Val Ala Val Lys Lys Asp Gly Val Val Leu Gly Thr Ser Asp Met
120 125 130
gcg agg tcc ctg cct cga acc gcc gct ggc caa gaa gcc tat gag tac 547
Ala Arg Ser Leu Pro Arg Thr Ala Ala Gly Gln Glu Ala Tyr Glu Tyr
135 140 145
ttc ttc aag gtg gtt cgt gaa ggc atc atc ggg cag ctg cgc ccg ggc 595
Phe Phe Lys Val Val Arg Glu Gly Ile Ile Gly Gln Leu Arg Pro Gly
150 155 160 165
gtg atc tgc gct gac gtg cac gaa gca acc ctt gat tac cta agc ccg 643
Val Ile Cys Ala Asp Val His Glu Ala Thr Leu Asp Tyr Leu Ser Pro
170 175 180
cag cta cct cgc atg att gac atc gga atg ctg ggt gcc gac acc gat 691
Gln Leu Pro Arg Met Ile Asp Ile Gly Met Leu Gly Ala Asp Thr Asp
185 190 195
ttc aac acc atc tac cgc aag cgc aat gtt ggc cac ctc atg ggc aag 739
Phe Asn Thr Ile Tyr Arg Lys Arg Asn Val Gly His Leu Met Gly Lys
200 205 210
cag gaa tcc ttt gcc aat gag ctt cgc cct gga tac aag cac att ctt 787
Gln Glu Ser Phe Ala Asn Glu Leu Arg Pro Gly Tyr Lys His Ile Leu
215 220 225
cac cac ggc tcc tat ggt gcc gcg gag atc cct tgg cgc tac aac ggt 835
His His Gly Ser Tyr Gly Ala Ala Glu Ile Pro Trp Arg Tyr Asn Gly
230 235 240 245
gta gcc att ggt acc gag gat ctg tgg tac atc ggc gca gac aag acc 883
Val Ala Ile Gly Thr Glu Asp Leu Trp Tyr Ile Gly Ala Asp Lys Thr
250 255 260
tac att ttg agc cag cgc taaggagaac ccagtgacag aaattgcaga 931
Tyr Ile Leu Ser Gln Arg
265
<210>42
<21l>267
<212>PRT
<213>谷氨酸棒状杆菌
<400>42
Leu Ser Leu Thr Glu Gly Glu Ile Ile Pro Ile Gln Thr Lys Leu Gly
1 5 10 15
His Trp Arg Asp Val Arg Asp Ser Glu Asp Leu Pro Phe Gln Val Ile
20 25 30
Ala Ser Arg Thr Ser Val Thr Ile Met Glu Asn Thr Leu Glu Trp Thr
35 40 45
Asn Ser Arg Leu Glu Arg Gly Glu Glu Phe Thr Glu Leu Asp Ile Tyr
50 55 60
Ala His Tyr Leu Glu Glu Leu Glu Asp Tyr Ala Leu Gln Phe Thr Phe
65 70 75 80
Asp Val Glu Pro Tyr Phe Thr Asn Leu His Ser Ser Asn Arg Met Leu
85 90 95
Phe Pro Gly Pro Pro Val Asp Phe Pro Ile Asn Ala Glu Thr Arg Cys
100 105 110
Ile Gln Leu Asp Ala Gly Val Ala Val Lys Lys Asp Gly Val Val Leu
115 120 125
Gly Thr Ser Asp Met Ala Arg Ser Leu Pro Arg Thr Ala Ala Gly Gln
130 135 140
Glu Ala Tyr Glu Tyr Phe Phe Lys Val Val Arg Glu Gly Ile Ile Gly
145 150 155 160
Gln Leu Arg Pro Gly Val Ile Cys Ala Asp Val His Glu Ala Thr Leu
165 170 175
Asp Tyr Leu Ser Pro Gln Leu Pro Arg Met Ile Asp Ile Gly Met Leu
180 185 190
Gly Ala Asp Thr Asp Phe Asn Thr Ile Tyr Arg Lys Arg Asn Val Gly
195 200 205
His Leu Met Gly Lys Gln Glu Ser Phe Ala Asn Glu Leu Arg Pro Gly
210 215 220
Tyr Lys His Ile Leu His His Gly Ser Tyr Gly Ala Ala Glu Ile Pro
225 230 235 240
Trp Arg Tyr Asn Gly Val Ala Ile Gly Thr Glu Asp Leu Trp Tyr Ile
245 250 255
Gly Ala Asp Lys Thr Tyr Ile Leu Ser Gln Arg
260 265
<210>43
<211>1543
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1513)
<223>RXA00409
<400>43
cggcgtcctc tgactccacc attcaaggcc tgcctgtcgc ggaagccgat gccttacgtg 60
agcgactggc tattaaggcc cgggagagga tgagcggact atg agc agc ctt gaa 115
Met Ser Ser Leu Glu
1 5
ggg ttt cga aaa gtc cac cgc gcc acc cca ttc ctt cgc atc tgg acc 163
Gly Phe Arg Lys Val His Arg Ala Thr Pro Phe Leu Arg Ile Trp Thr
10 15 20
atc atc gtc gca gtt ctc gcg gca ttc gca ttc aat tcc ggc gca tcc 211
Ile Ile Val Ala Val Leu Ala Ala Phe Ala Phe Asn Ser Gly Ala Ser
25 30 35
gtt tta agt ttc atc tgg ggt gtc gtc acc ggc gaa tac gga ttc gca 259
Val Leu Ser Phe Ile Trp Gly Val Val Thr Gly Glu Tyr Gly Phe Ala
40 45 50
gtg ctg ccg att cta ttg acc gtc ggt ggc gcc gtc att gtg gtg gca 307
Val Leu Pro Ile Leu Leu Thr Val Gly Gly Ala Val Ile Val Val Ala
55 60 65
ctg gcc tgg att atc acc gga att tgg tgg aaa gcc gtc gga ttc cgc 355
Leu Ala Trp Ile Ile Thr Gly Ile Trp Trp Lys Ala Val Gly Phe Arg
70 75 80 85
atc acc aat gaa gag gtg cag ctg caa cgc gga gtg atc agc aaa gac 403
Ile Thr Asn Glu Glu Val Gln Leu Gln Arg Gly Val Ile Ser Lys Asp
90 95 100
ctc cgc acc gcc cga ttt gac cgt atc caa gcc gtt gac ctg gtg gaa 451
Leu Arg Thr Ala Arg Phe Asp Arg Ile Gln Ala Val Asp Leu Val Glu
105 110 115
tca ttc atc gcc cgt atc ttc cga ctg gca gaa gtc cgc atc gaa acc 499
Ser Phe Ile Ala Arg Ile Phe Arg Leu Ala Glu Val Arg Ile Glu Thr
120 125 130
gcc ggc ggc agc gac tcc gcc atc agc att ggc ttt tta aga aaa agc 547
Ala Gly Gly Ser Asp Ser Ala Ile Ser Ile Gly Phe Leu Arg Lys Ser
135 140 145
gag gcc gag gcc cta aaa agg gag ctt ctc gac gcc tcc cag cac tca 595
Glu Ala Glu Ala Leu Lys Arg Glu Leu Leu Asp Ala Ser Gln His Ser
150 155 160 165
gtc gcc acc acc cca gcc gga gtt ccg gct gag cct ggg gta ggg gag 643
Val Ala Thr Thr Pro Ala Gly Val Pro Ala Glu Pro Gly Val Gly Glu
170 175 180
acc gtt gta gtt gag agc gcg ggc gat gtt ctg gtt cca caa atc ccc 691
Thr Val Val Val Glu Ser Ala Gly Asp Val Leu Val Pro Gln Ile Pro
185 190 195
gtg caa cgc acc ctc gcc agc acc gcc tta tca cta gca acc atc atc 739
Val Gln Arg Thr Leu Ala Ser Thr Ala Leu Ser Leu Ala Thr Ile Ile
200 205 210
acc gcg atc ggt att gtg att ttg ctc ttc gtt cct ttc gga gtg agc 787
Thr Ala Ile Gly Ile Val Ile Leu Leu Phe Val Pro Phe Gly Val Ser
215 220 225
att gcc gtg ccg ttc ttt gta ggc atg gtg cca gcg gtc tgg aac ttg 835
Ile Ala Val Pro Phe Phe Val Gly Met Val Pro Ala Val Trp Asn Leu
230 235 240 245
atc gat aag tcg tgg caa ttc acc gca aca cag cgc aac gat gtc ctg 883
Ile Asp Lys Ser Trp Gln Phe Thr Ala Thr Gln Arg Asn Asp Val Leu
250 255 260
cat gtc agc tat gga ctt gcc aac cgc cgc aaa caa tca atc cca ctg 931
His Val Ser Tyr Gly Leu Ala Asn Arg Arg Lys Gln Ser Ile Pro Leu
265 270 275
gga cgc atc cac gca gtg aaa cta aaa cag cca ttg ctg tgg cga ctg 979
Gly Arg Ile His Ala Val Lys Leu Lys Gln Pro Leu Leu Trp Arg Leu
280 285 290
gtt ggc tgg tgg acc gtg acc gtg act gta gtt ggc tac ggc gac acc 1027
Val Gly Trp Trp Thr Val Thr Val Thr Val Val Gly Tyr Gly Asp Thr
295 300 305
acc caa ggc gga acc tcc aaa atc ttg ccc gtg ggt tcc aaa gaa ctc 1075
Thr Gln Gly Gly Thr Ser Lys Ile Leu Pro Val Gly Ser Lys Glu Leu
310 315 320 325
gca cta aaa gtc ctt gaa gcg gtg ggg cca cta aac agc gcc gac atc 1123
Ala Leu Lys Val Leu Glu Ala Val Gly Pro Leu Asn Ser Ala Asp Ile
330 335 340
gcc gaa tct gca gac cca tca cat atg agc aga ccc cag tac aca cca 1171
Ala Glu Ser Ala Asp Pro Ser His Met Ser Arg Pro Gln Tyr Thr Pro
345 350 355
cca gtt gct gca cgc ctg ctc aca cca gtc gat cgg acc cgc caa ggc 1219
Pro Val Ala Ala Arg Leu Leu Thr Pro Val Asp Arg Thr Arg Gln Gly
360 365 370
gtc acg ttg att ggt gtg gct ggt gcc cct ggt gcg gtg gtc gtg cat 1267
Val Thr Leu Ile Gly Val Ala Gly Ala Pro Gly Ala Val Val Val His
375 380 385
gaa ggc aga ttc atg cca cgt atg tct gtg att gat acc tcc cac atc 1315
Glu Gly Arg Phe Met Pro Arg Met Ser Val Ile Asp Thr Ser His Ile
390 395 400 405
caa gaa ctc acc ctt aaa cac ggg ccg atc caa aga ata ctg gga ctc 1363
Gln Glu Leu Thr Leu Lys His Gly Pro Ile Gln Arg Ile Leu Gly Leu
410 415 420
tcc acc gtt gtg ttt aac ctg gtc caa ggg cca gtg gga atg gcc gca 1411
Ser Thr Val Val Phe Asn Leu Val Gln Gly Pro Val Gly Met Ala Ala
425 430 435
tcg gat ctc agt gca gct gat gga aaa gaa ctt ctc aac att ctc cgc 1459
Ser Asp Leu Ser Ala Ala Asp Gly Lys Glu Leu Leu Asn Ile Leu Arg
440 445 450
aac aga aaa cta ccc gca ctg gaa tca gct ccg ctg gga caa aac agc 1507
Asn Arg Lys Leu Pro Ala Leu Glu Ser Ala Pro Leu Gly Gln Asn Ser
455 460 465
ctg gac taaggtgtaa tcatgcacat ctcagatctt 1543
Leu Asp
470
<210>44
<211>471
<212>PRT
<213>谷氨酸棒状杆菌
<400>44
Met Ser Ser Leu Glu Gly Phe Arg Lys Val His Arg Ala Thr Pro Phe
1 5 10 15
Leu Arg Ile Trp Thr Ile Ile Val Ala Val Leu Ala Ala Phe Ala Phe
20 25 30
Asn Ser Gly Ala Ser Val Leu Ser Phe Ile Trp Gly Val Val Thr Gly
35 40 45
Glu Tyr Gly Phe Ala Val Leu Pro Ile Leu Leu Thr Val Gly Gly Ala
50 55 60
Val Ile Val Val Ala Leu Ala Trp Ile Ile Thr Gly Ile Trp Trp Lys
65 70 75 80
Ala Val Gly Phe Arg Ile Thr Asn Glu Glu Val Gln Leu Gln Arg Gly
85 90 95
Val Ile Ser Lys Asp Leu Arg Thr Ala Arg Phe Asp Arg Ile Gln Ala
100 105 110
Val Asp Leu Val Glu Ser Phe Ile Ala Arg Ile Phe Arg Leu Ala Glu
115 120 125
Val Arg Ile Glu Thr Ala Gly Gly Ser Asp Ser Ala Ile Ser Ile Gly
130 135 140
Phe Leu Arg Lys Ser Glu Ala Glu Ala Leu Lys Arg Glu Leu Leu Asp
145 150 155 160
Ala Ser Gln His Ser Val Ala Thr Thr Pro Ala Gly Val Pro Ala Glu
165 170 175
Pro Gly Val Gly Glu Thr Val Val Val Glu Ser Ala Gly Asp Val Leu
180 185 190
Val Pro Gln Ile Pro Val Gln Arg Thr Leu Ala Ser Thr Ala Leu Ser
195 200 205
Leu Ala Thr Ile Ile Thr Ala Ile Gly Ile Val Ile Leu Leu Phe Val
2l0 215 220
Pro Phe Gly Val Ser Ile Ala Val Pro Phe Phe Val Gly Met Val Pro
225 230 235 240
Ala Val Trp Asn Leu Ile Asp Lys Ser Trp Gln Phe Thr Ala Thr Gln
245 250 255
Arg Asn Asp Val Leu His Val Ser Tyr Gly Leu Ala Asn Arg Arg Lys
260 265 270
Gln Ser Ile Pro Leu Gly Arg Ile His Ala Val Lys Leu Lys Gln Pro
275 280 285
Leu Leu Trp Arg Leu Val Gly Trp Trp Thr Val Thr Val Thr Val Val
290 295 300
Gly Tyr Gly Asp Thr Thr Gln Gly Gly Thr Ser Lys Ile Leu Pro Val
305 310 315 320
Gly Ser Lys Glu Leu Ala Leu Lys Val Leu Glu Ala Val Gly Pro Leu
325 330 335
Asn Ser Ala Asp Ile Ala Glu Ser Ala Asp Pro Ser His Met Ser Arg
340 345 350
Pro Gln Tyr Thr Pro Pro Val Ala Ala Arg Leu Leu Thr Pro Val Asp
355 360 365
Arg Thr Arg Gln Gly Val Thr Leu Ile Gly Val Ala Gly Ala Pro Gly
370 375 380
Ala Val Val Val His Glu Gly Arg Phe Met Pro Arg Met Ser Val Ile
385 390 395 400
Asp Thr Ser His Ile Gln Glu Leu Thr Leu Lys His Gly Pro Ile Gln
405 410 415
Arg Ile Leu Gly Leu Ser Thr Val Val Phe Asn Leu Val Gln Gly Pro
420 425 430
Val Gly Met Ala Ala Ser Asp Leu Ser Ala Ala Asp Gly Lys Glu Leu
435 440 445
Leu Asn Ile Leu Arg Asn Arg Lys Leu Pro Ala Leu Glu Ser Ala Pro
450 455 460
Leu Gly Gln Asn Ser Leu Asp
465 470
<210>45
<211>2161
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2131)
<223>RXA00416
<400>45
caagctctga aagcagatag cacgtgagac tcattaatac atacttgtaa ttttgctcct 60
cgatcccagc aatgctcgtt gagtatgaaa gacttctatc atg att aac acc tct 115
Met Ile Asn Thr Ser
1 5
gaa gat gaa ggt gca gtc gtg gtt cat gag gat ttg aat cct tat ggt 163
Glu Asp Glu Gly Ala Val Val Val His Glu Asp Leu Asn Pro Tyr Gly
10 15 20
cca tca cgt gtg cct gaa ggc gaa gtg tat cgg cct gat cgt cta aac 211
Pro Ser Arg Val Pro Glu Gly Glu Val Tyr Arg Pro Asp Arg Leu Asn
25 30 35
agg aaa gct aca ctt gtt gca att gtg gga gct gcc atc ctg gct ttc 259
Arg Lys Ala Thr Leu Val Ala Ile Val Gly Ala Ala Ile Leu Ala Phe
40 45 50
gca ttt gct ttg gtg ttg tgg atg ggt tta aaa caa aca aac ctt cca 307
Ala Phe Ala Leu Val Leu Trp Met Gly Leu Lys Gln Thr Asn Leu Pro
55 60 65
gct ttc ggc cca tcg aat gtc acg agg gct gtt gct tct gcc acc atc 355
Ala Phe Gly Pro Ser Asn Val Thr Arg Ala Val Ala Ser Ala Thr Ile
70 75 80 85
gct gcg gtt ttg att gtc acg ggt ttt ttg acg tgg ctg tgg ctg cgc 403
Ala Ala Val Leu Ile Val Thr Gly Phe Leu Thr Trp Leu Trp Leu Arg
90 95 100
gat gaa cat cag agc aat ccg cgg tgg gag ttg gag gac gtc aag cca 451
Asp Glu His Gln Ser Asn Pro Arg Trp Glu Leu Glu Asp Val Lys Pro
105 110 115
agg ccc aag tgg cgg acg gcg ctg acg tat ctg gcg tct tac tta agc 499
Arg Pro Lys Trp Arg Thr Ala Leu Thr Tyr Leu Ala Ser Tyr Leu Ser
120 125 130
cca gct gcg ctg gtg gtt gcg gtg ttg gct att ccg ctg tct gcg acc 547
Pro Ala Ala Leu Val Val Ala Val Leu Ala Ile Pro Leu Ser Ala Thr
135 140 145
cgc ctg tat ttg gac gga atc agc gtt gac cag ggc ttt aga act cag 595
Arg Leu Tyr Leu Asp Gly Ile Ser Val Asp Gln Gly Phe Arg Thr Gln
150 155 160 165
ttt tta acc cgc atg gct gac gat atc ggc ttg tcg gac atg aac tac 643
Phe Leu Thr Arg Met Ala Asp Asp Ile Gly Leu Ser Asp Met Asn Tyr
170 175 180
atc gat atg cct acc ttc tac cct gct gga tgg ttc tgg ctc ggt ggt 691
Ile Asp Met Pro Thr Phe Tyr Pro Ala Gly Trp Phe Trp Leu Gly Gly
185 190 195
cgc ttg gcc aat ctt ttg ggg ctg ccc ggt tgg gaa gct ttc cag cca 739
Arg Leu Ala Asn Leu Leu Gly Leu Pro Gly Trp Glu Ala Phe Gln Pro
200 205 210
tgg gca att gtg tcc atg gca gtt gct gct tct gtg tta gtt cca gtg 787
Trp Ala Ile Val Ser Met Ala Val Ala Ala Ser Val Leu Val Pro Val
215 220 225
tgg cag cgc atc acc ggt tcc ctg ccg gtg gca aca ggc att gcg ttg 835
Trp Gln Arg Ile Thr Gly Ser Leu Pro Val Ala Thr Gly Ile Ala Leu
230 235 240 245
gtg aca acc tgc att atc ttg gcg atg aat tcc gaa gag ccc tac gct 883
Val Thr Thr Cys Ile Ile Leu Ala Met Asn Ser Glu Glu Pro Tyr Ala
250 255 260
gca atc gtt gcg atg ggt att cca gcg atg ctc gtg ctg gct tcc cgc 931
Ala Ile Val Ala Met Gly Ile Pro Ala Met Leu Val Leu Ala Ser Arg
265 270 275
att gcc aag ggc gat aag ttt gcg ctt gcc ggc ggc att att tac ttg 979
Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala Gly Gly Ile Ile Tyr Leu
280 285 290
ggt gtt tcg gct act ttc tat act ttg ttc acc ggt gct atc gcg ctt 1027
Gly Val Ser Ala Thr Phe Tyr Thr Leu Phe Thr Gly Ala Ile Ala Leu
295 300 305
tct gcg gtc gcg gtg tgc atc gtg gtg gcg gct att gtg cag cgc tcc 1075
Ser Ala Val Ala Val Cys Ile Val Val Ala Ala Ile Val Gln Arg Ser
310 315 320 325
atc aaa cca ctg ctg tgg ctt gca gtg ctg ggt ggt gga tcc att gtc 1123
Ile Lys Pro Leu Leu Trp Leu Ala Val Leu Gly Gly Gly Ser Ile Val
330 335 340
att gcg ttg att tct tgg ggt cct tac ctt ctg gcc tcc atc aac gga 1171
Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu Leu Ala Ser Ile Asn Gly
345 350 355
gcg gag cgc tct ggc gat tcc gca aca cac tac ctg cct ctt gaa ggc 1219
Ala Glu Arg Ser Gly Asp Ser Ala Thr His Tyr Leu Pro Leu Glu Gly
360 365 370
acc caa ttc ccg gtt cct ttc ttg gca tca agc gtt gtg gga ctg ttg 1267
Thr Gln Phe Pro Val Pro Phe Leu Ala Ser Ser Val Val Gly Leu Leu
375 380 385
tgt ctt gtt ggc ctg atc tat ttg gtg gtg cgt ttc cac aac aat gag 1315
Cys Leu Val Gly Leu Ile Tyr Leu Val Val Arg Phe His Asn Asn Glu
390 395 400 405
gtg cgc gcg atg tgg gtc ggc atc gca gtg ttt tat gcc tgg atg ggc 1363
Val Arg Ala Met Trp Val Gly Ile Ala Val Phe Tyr Ala Trp Met Gly
410 415 420
atg tcc atg gcg atc acg ctt ttg ggc aac acg ttg ctt gga ttc cgt 1411
Met Ser Met Ala Ile Thr Leu Leu Gly Asn Thr Leu Leu Gly Phe Arg
425 430 435
ctt gat acg gtg ctg gtg ctt att ttt gcc acg gct gga gtg ttg ggc 1459
Leu Asp Thr Val Leu Val Leu Ile Phe Ala Thr Ala Gly Val Leu Gly
440 445 450
att gca gat ttc cgc ctt gcc agt gtg tat cag ctc tac ccc acc caa 1507
Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr Gln Leu Tyr Pro Thr Gln
455 460 465
atc aca gag cgc acg gcc acc cat ctg acc aat cta att gtg gtc ctc 1555
Ile Thr Glu Arg Thr Ala Thr His Leu Thr Asn Leu Ile Val Val Leu
470 475 480 485
gtg ctg ctt ggc ggc ctc tac tac gcg caa gat ctg ccg cag aag aac 1603
Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gln Asp Leu Pro Gln Lys Asn
490 495 500
gca cga gct atc gat ctg gcc tat acc gat act gat ggc tac ggc gag 1651
Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp Thr Asp Gly Tyr Gly Glu
505 510 515
cgc gcg gat ctg tat ccg gcc gga gct gca cgt tat tac aag gac atc 1699
Arg Ala Asp Leu Tyr Pro Ala Gly Ala Ala Arg Tyr Tyr Lys Asp Ile
520 525 530
aac gat cat ctg ctt gat caa gga ttc gag cct tcc gaa act gtc gtg 1747
Asn Asp His Leu Leu Asp Gln Gly Phe Glu Pro Ser Glu Thr Val Val
535 540 545
ctg aca gac gaa ctc gat ttc atg tcc tac tac cct tat cgc gga tac 1795
Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr Tyr Pro Tyr Arg Gly Tyr
550 555 560 565
caa gct ttt act tcc cac tac gcc aac ccg ctt ggt gag ttc gga aac 1843
Gln Ala Phe Thr Ser His Tyr Ala Asn Pro Leu Gly Glu Phe Gly Asn
570 575 580
agg aac gca ttc atc gaa gat ctc gcg atc cga agc tgg gat gag ttg 1891
Arg Asn Ala Phe Ile Glu Asp Leu Ala Ile Arg Ser Trp Asp Glu Leu
585 590 595
gct gat cct caa caa ttc agc gac gcc ttg aac acc tct cca tgg acg 1939
Ala Asp Pro Gln Gln Phe Ser Asp Ala Leu Asn Thr Ser Pro Trp Thr
600 605 610
atc cct gag gtg ttc atc ttc cgt ggc tcc atc gat gat cct gac gcc 1987
Ile Pro Glu Val Phe Ile Phe Arg Gly Ser Ile Asp Asp Pro Asp Ala
615 620 625
ggt tgg aaa tac gac gtg gct gaa gat ctg tac ccg aac aat cca aac 2035
Gly Trp Lys Tyr Asp Val Ala Glu Asp Leu Tyr Pro Asn Asn Pro Asn
630 635 640 645
gtg cgc ttc cgc ggc gtg tac ttt aac ccg gag tca ttt gat cag atg 2083
Val Arg Phe Arg Gly Val Tyr Phe Asn Pro Glu Ser Phe Asp Gln Met
650 655 660
tgg cag acc aag caa gtg gga cct ttc gtg gtg gta acg cac aat gag 2131
Trp Gln Thr Lys Gln Val Gly Pro Phe Val Val Val Thr His Asn Glu
665 670 675
taattcctca ccaaacgacc caagccctat 2161
<210>46
<211>677
<212>PRT
<213>谷氨酸棒状杆菌
<400>46
Met Ile Asn Thr Ser Glu Asp Glu Gly Ala Val Val Val His Glu Asp
1 5 10 15
Leu Asn Pro Tyr Gly Pro Ser Arg Val Pro Glu Gly Glu Val Tyr Arg
20 25 30
Pro Asp Arg Leu Asn Arg Lys Ala Thr Leu Val Ala Ile Val Gly Ala
35 40 45
Ala Ile Leu Ala Phe Ala Phe Ala Leu Val Leu Trp Met Gly Leu Lys
50 55 60
Gln Thr Asn Leu Pro Ala Phe Gly Pro Ser Asn Val Thr Arg Ala Val
65 70 75 80
Ala Ser Ala Thr Ile Ala Ala Val Leu Ile Val Thr Gly Phe Leu Thr
85 90 95
Trp Leu Trp Leu Arg Asp Glu His Gln Ser Asn Pro Arg Trp Glu Leu
100 105 110
Glu Asp Val Lys Pro Arg Pro Lys Trp Arg Thr Ala Leu Thr Tyr Leu
115 120 125
Ala Ser Tyr Leu Ser Pro Ala Ala Leu Val Val Ala Val Leu Ala Ile
130 135 140
Pro Leu Ser Ala Thr Arg Leu Tyr Leu Asp Gly Ile Ser Val Asp Gln
145 150 155 160
Gly Phe Arg Thr Gln Phe Leu Thr Arg Met Ala Asp Asp Ile Gly Leu
165 170 175
Ser Asp Met Asn Tyr Ile Asp Met Pro Thr Phe Tyr Pro Ala Gly Trp
180 185 190
Phe Trp Leu Gly Gly Arg Leu Ala Asn Leu Leu Gly Leu Pro Gly Trp
195 200 205
Glu Ala Phe Gln Pro Trp Ala Ile Val Ser Met Ala Val Ala Ala Ser
210 215 220
Val Leu Val Pro Val Trp Gln Arg Ile Thr Gly Ser Leu Pro Val Ala
225 230 235 240
Thr Gly Ile Ala Leu Val Thr Thr Cys Ile Ile Leu Ala Met Asn Ser
245 250 255
Glu Glu Pro Tyr Ala Ala Ile Val Ala Met Gly Ile Pro Ala Met Leu
260 265 270
Val Leu Ala Ser Arg Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala Gly
275 280 285
Gly Ile Ile Tyr Leu Gly Val Ser Ala Thr Phe Tyr Thr Leu Phe Thr
290 295 300
Gly Ala Ile Ala Leu Ser Ala Val Ala Val Cys Ile Val Val Ala Ala
305 310 315 320
Ile Val Gln Arg Ser Ile Lys Pro Leu Leu Trp Leu Ala Val Leu Gly
325 330 335
Gly Gly Ser Ile Val Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu Leu
340 345 350
Ala Ser Ile Asn Gly Ala Glu Arg Ser Gly Asp Ser Ala Thr His Tyr
355 360 365
Leu Pro Leu Glu Gly Thr Gln Phe Pro Val Pro Phe Leu Ala Ser Ser
370 375 380
Val Val Gly Leu Leu Cys Leu Val Gly Leu Ile Tyr Leu Val Val Arg
385 390 395 400
Phe His Asn Asn Glu Val Arg Ala Met Trp Val Gly Ile Ala Val Phe
405 410 415
Tyr Ala Trp Met Gly Met Ser Met Ala Ile Thr Leu Leu Gly Asn Thr
420 425 430
Leu Leu Gly Phe Arg Leu Asp Thr Val Leu Val Leu Ile Phe Ala Thr
435 440 445
Ala Gly Val Leu Gly Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr Gln
450 455 460
Leu Tyr Pro Thr Gln Ile Thr Glu Arg Thr Ala Thr His Leu Thr Asn
465 470 475 480
Leu Ile Val Val Leu Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gln Asp
485 490 495
Leu Pro Gln Lys Asn Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp Thr
500 505 510
Asp Gly Tyr Gly Glu Arg Ala Asp Leu Tyr Pro Ala Gly Ala Ala Arg
515 520 525
Tyr Tyr Lys Asp Ile Asn Asp His Leu Leu Asp Gln Gly Phe Glu Pro
530 535 540
Ser Glu Thr Val Val Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr Tyr
545 550 555 560
Pro Tyr Arg Gly Tyr Gln Ala Phe Thr Ser His Tyr Ala Asn Pro Leu
565 570 575
Gly Glu Phe Gly Asn Arg Asn Ala Phe Ile Glu Asp Leu Ala Ile Arg
580 585 590
Ser Trp Asp Glu Leu Ala Asp Pro Gln Gln Phe Ser Asp Ala Leu Asn
595 600 605
Thr Ser Pro Trp Thr Ile Pro Glu Val Phe Ile Phe Arg Gly Ser Ile
610 615 620
Asp Asp Pro Asp Ala Gly Trp Lys Tyr Asp Val Ala Glu Asp Leu Tyr
625 630 635 640
Pro Asn Asn Pro Asn Val Arg Phe Arg Gly Val Tyr Phe Asn Pro Glu
645 650 655
Ser Phe Asp Gln Met Trp Gln Thr Lys Gln Val Gly Pro Phe Val Val
660 665 670
Val Thr His Asn Glu
675
<210>47
<211>619
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(589)
<223>RXA00424
<400>47
aggttggtgc gttccgccgc gatgtggacc tcgggagcct tggctttttt gaatctttca 60
aacatgccca ccaccttaat cagtgagtag tcttggcaac atg gat acc agg atg 115
Met Asp Thr Arg Met
1 5
aga ttg cag gta gca gca ggt tcc gcg ctg att ggt gct ggg gtt gcg 163
Arg Leu Gln Val Ala Ala Gly Ser Ala Leu Ile Gly Ala Gly Val Ala
10 15 20
gtt aat gat tac gta caa agc cca gtc cgc cgg gcc atc agt tat ggt 211
Val Asn Asp Tyr Val Gln Ser pro Val Arg Arg Ala Ile Ser Tyr Gly
25 30 35
gcg ttg gcg ctt tct ggg gcg acg gtg att gcg atg ggt cag gac ccg 259
Ala Leu Ala Leu Ser Gly Ala Thr Val Ile Ala Met Gly Gln Asp Pro
40 45 50
act ggg gag cgg tcc atc att gcg aaa gat tcc gcg acg atg gtt gat 307
Thr Gly Glu Arg Set Ile Ile Ala Lys Asp Ser Ala Thr Met Val Asp
55 60 65
caa atc cgc cag gag atc ggc gat ttg ggt gtc acg cct ggt cca gaa 355
Gln Ile Arg Gln Glu Ile Gly Asp Leu Gly Val Thr Pro Gly Pro Glu
70 75 80 85
tct gat gtg gat gcc atc acg gag cga ggt ccg ctg gtg acg tgg ctg 403
Ser Asp Val Asp Ala Ile Thr Glu Arg Gly Pro Leu Val Thr Trp Leu
90 95 100
ttg ctt gct gtt ttt gtc gtg gca ttt ttc acc ttg gcg tat ttc tcc 451
Leu Leu Ala Val Phe Val Val Ala Phe Phe Thr Leu Ala Tyr Phe Ser
105 110 115
atg cgc atg gat gtg gcg gtg atg aga agg atc gct aag ttc ttt gaa 499
Met Arg Met Asp Val Ala Val Met Arg Arg Ile Ala Lys Phe Phe Glu
120 125 130
aaa cgt ggc gct tcc agg cca ttc acc tgc act ggc atg gtg tac gcc 547
Lys Arg Gly Ala Ser Arg Pro Phe Thr Cys Thr Gly Met Val Tyr Ala
135 140 145
gct ttt att tac gca atc tgt gag ctg gag gcc cgc aca aaa 589
Ala Phe Ile Tyr Ala Ile Cys Glu Leu Glu Ala Arg Thr Lys
150 155 160
tgacctatcc cgtgttgagt gttttggata 619
<210>48
<211>163
<212>PRT
<213>谷氨酸棒状杆菌
<400>48
Met Asp Thr Arg Met Arg Leu Gln Val Ala Ala Gly Ser Ala Leu Ile
1 5 10 15
Gly Ala Gly Val Ala Val Asn Asp Tyr Val Gln Ser Pro Val Arg Arg
20 25 30
Ala Ile Ser Tyr Gly Ala Leu Ala Leu Ser Gly Ala Thr Val Ile Ala
35 40 45
Met Gly Gln Asp Pro Thr Gly Glu Arg Ser Ile Ile Ala Lys Asp Ser
50 55 60
Ala Thr Met Val Asp Gln Ile Arg Gln Glu Ile Gly Asp Leu Gly Val
65 70 75 80
Thr Pro Gly Pro Glu Ser Asp Val Asp Ala Ile Thr Glu Arg Gly Pro
85 90 95
Leu Val Thr Trp Leu Leu Leu Ala Val Phe Val Val Ala Phe Phe Thr
100 105 110
Leu Ala Tyr Phe Ser Met Arg Met Asp Val Ala Val Met Arg Arg Ile
115 120 125
Ala Lys Phe Phe Glu Lys Arg Gly Ala Ser Arg Pro Phe Thr Cys Thr
130 135 140
Gly Met Val Tyr Ala Ala Phe Ile Tyr Ala Ile Cys Glu Leu Glu Ala
145 150 155 160
Arg Thr Lys
<210>49
<211>652
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(622)
<223>RXA00451
<400>49
gtggttggct gcatggaggt cgcggaggag ggatccgccg atgaggccga ggcccaggat 60
gcatactggg cgggaaatgt ctttggtagt cacctgacaa gtg tgg cac ata cct 115
Val Trp His Ile Pro
1 5
act acg gtt atc tct atg aac act gaa gag gat ggc ctg agc ttt gca 163
Thr Thr Val Ile Ser Met Asn Thr Glu Glu Asp Gly Leu Ser Phe Ala
10 15 20
gtt acg gct acc tta gtt gaa ggt aaa tgg cag gta cgc gag ttt gag 211
Val Thr Ala Thr Leu Val Glu Gly Lys Trp Gln Val Arg Glu Phe Glu
25 30 35
gat cac ttc tcc aag ctc tct acc tct att aat gcg gtt cgc tcg ctg 259
Asp His Phe Ser Lys Leu Ser Thr Ser Ile Asn Ala Val Arg Ser Leu
40 45 50
cgt agc gag ggg ccg gcg ttt gcg ctg ttg tgc gtt gat gat gaa tat 307
Arg Ser Glu Gly Pro Ala Phe Ala Leu Leu Cys Val Asp Asp Glu Tyr
55 60 65
ttt gtg atg gtg cgt ccc acc cca agt agg gta ttc ctt ttc ctt tcg 355
Phe Val Met Val Arg Pro Thr Pro Ser Arg Val Phe Leu Phe Leu Ser
70 75 80 85
gat gcg ccg atg gct gtc gac gat gat ttc gcc gcc gcg gtg atg gat 403
Asp Ala Pro Met Ala Val Asp Asp Asp Phe Ala Ala Ala Val Met Asp
90 95 100
gaa ctc gat gcg gat ctt cca gat atc aac cct gat gat ttg gac gac 451
Glu Leu Asp Ala Asp Leu Pro Asp Ile Asn Pro Asp Asp Leu Asp Asp
105 110 115
atc gat cca tgg cca gaa ggt gat ttc gat att ttg gcg gat ttg ggg 499
Ile Asp Pro Trp Pro Glu Gly Asp Phe Asp Ile Leu Ala Asp Leu Gly
120 125 130
ctg tct gag gag gtt ctt tcc gtg att tgc gat gac atg gat ttg gat 547
Leu Ser Glu Glu Val Leu Ser Val Ile Cys Asp Asp Met Asp Leu Asp
135 140 145
cct tcc gaa caa ttg ctg cgc atc gcg gaa gaa ctc ggt ttt gat aat 595
Pro Ser Glu Gln Leu Leu Arg Ile Ala Glu Glu Leu Gly Phe Asp Asn
150 155 160 165
gat ctg gct cgg gtg gtg gga ttc gac tagtgggcgt tttacctgtg 642
Asp Leu Ala Arg Val Val Gly Phe Asp
170
caggcgcgca 652
<210>50
<211>174
<212>PRT
<213>谷氨酸棒状杆菌
<400>50
Val Trp His Ile Pro Thr Thr Val Ile Ser Met Asn Thr Glu Glu Asp
1 5 10 15
Gly Leu Ser Phe Ala Val Thr Ala Thr Leu Val Glu Gly Lys Trp Gln
20 25 30
Val Arg Glu Phe Glu Asp His Phe Ser Lys Leu Ser Thr Ser Ile Asn
35 40 45
Ala Val Arg Ser Leu Arg Ser Glu Gly Pro Ala Phe Ala Leu Leu Cys
50 55 60
Val Asp Asp Glu Tyr Phe Val Met Val Arg Pro Thr Pro Ser Arg Val
65 70 75 80
Phe Leu Phe Leu Ser Asp Ala Pro Met Ala Val Asp Asp Asp Phe Ala
85 90 95
Ala Ala Val Met Asp Glu Leu Asp Ala Asp Leu Pro Asp Ile Asn Pro
100 105 110
Asp Asp Leu Asp Asp Ile Asp Pro Trp Pro Glu Gly Asp Phe Asp Ile
115 120 125
Leu Ala Asp Leu Gly Leu Ser Glu Glu Val Leu Ser Val Ile Cys Asp
130 135 140
Asp Met Asp Leu Asp Pro Ser Glu Gln Leu Leu Arg Ile Ala Glu Glu
145 150 155 160
Leu Gly Phe Asp Asn Asp Leu Ala Arg Val Val Gly Phe Asp
165 170
<210>51
<211>991
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(961)
<223>RXA00476
<400>51
gtgaacgaat tgttgtaatt cgtcttaaaa acgccaggag acgtgaaaat tacagacacc 60
ccagacatca gatggaggcg gcgatactag ggtagaggac atg act ctt cgc tgt 115
Met Thr Leu Arg Cys
1 5
tct gac gtc aat gtt gaa ccc ctg ccg gga acg gca aaa aca ggt tct 163
Ser Asp Val Asn Val Glu Pro Leu Pro Gly Thr Ala Lys Thr Gly Ser
10 15 20
ggg ttt gtt ctc ctt gaa cat gct ggc tcg tgg agc cgt gat gtt tta 211
Gly Phe Val Leu Leu Glu His Ala Gly Ser Trp Ser Arg Asp Val Leu
25 30 35
gac ggc gga aca ttt gat cct gag ttg act gat caa ttg aag agg cac 259
Asp Gly Gly Thr Phe Asp Pro Glu Leu Thr Asp Gln Leu Lys Arg His
40 45 50
ctg aaa gct tcc gga atg ggt ctg caa tta att agg aag ccg gga agg 307
Leu Lys Ala Ser Gly Met Gly Leu Gln Leu Ile Arg Lys Pro Gly Arg
55 60 65
gag ggt cga aac gtc gaa aag cat aat ctt ttt ctc gtt ttt gct gag 355
Glu Gly Arg Asn Val Glu Lys His Asn Leu Phe Leu Val Phe Ala Glu
70 75 80 85
gcc tca att att gag cac ctg gtg gtg gac gcg ccg gct gat gtt ttg 403
Ala Ser Ile Ile Glu His Leu Val Val Asp Ala Pro Ala Asp Val Leu
90 95 100
gat ctt gat tta agc ggg ccg ggc aaa aac aat gcg cag cgc atg gat 451
Asp Leu Asp Leu Ser Gly Pro Gly Lys Asn Asn Ala Gln Arg Met Asp
105 110 115
gat ccg atg ctg ctg att tgt acg cat tcg aag cgc gat gtg tgc tgc 499
Asp Pro Met Leu Leu Ile Cys Thr His Ser Lys Arg Asp Val Cys Cys
120 125 130
gcg atc aag ggg cgt ccg ctg gca gct gcc gtg gag cca caa ttt ggg 547
Ala Ile Lys Gly Arg Pro Leu Ala Ala Ala Val Glu Pro Gln Phe Gly
135 140 145
ccg ctg cat gtg tgg gag gct tcg cac acc aag ggc cac cgt ttt gcg 595
Pro Leu His Val Trp Glu Ala Ser His Thr Lys Gly His Arg Phe Ala
150 155 160 165
cca tcg atg ctg ctc atg ccg tgg aat tac tct tat ggc cta ctt gat 643
Pro Ser Met Leu Leu Met Pro Trp Asn Tyr Ser Tyr Gly Leu Leu Asp
170 175 180
gag gcc gaa acc gtg cag ctt ttc caa ggc gcg ttg gac aac aaa ctc 691
Glu Ala Glu Thr Val Gln Leu Phe Gln Gly Ala Leu Asp Asn Lys Leu
185 190 195
ttc ctg ccg ggc aac cgt ggc cga gga acc tta gat gct cgt ggc cag 739
Phe Leu Pro Gly Asn Arg Gly Arg Gly Thr Leu Asp Ala Arg Gly Gln
200 205 210
gtt gca gaa att gcc gtg gcg gaa gct ttc ggc gag gcg gtt gct cct 787
Val Ala Glu Ile Ala Val Ala Glu Ala Phe Gly Glu Ala Val Ala Pro
215 220 225
gcg agt ttg cag gtt gaa ttc gaa gat gat tct gtt ttg gtt act cat 835
Ala Ser Leu Gln Val Glu Phe Glu Asp Asp Ser Val Leu Val Thr His
230 235 240 245
ccc gat ggg cgc acg tgg gtt gtg gag ctt gaa cgc atc gag gtc gac 883
Pro Asp Gly Arg Thr Trp Val Val Glu Leu Glu Arg Ile Glu Val Asp
250 255 260
ggc gtg gtg tcc tcg tgt ggt gat cag ccg aaa act gga aaa gcg tgg 931
Gly Val Val Ser Ser Cys Gly Asp Gln Pro Lys Thr Gly Lys Ala Trp
265 270 275
gtg gct agg caa gtt aca gaa ctg atc gga taaaagcaga gttatatctg 981
Val Ala Arg Gln Val Thr Glu Leu Ile Gly
280 285
atgaattgct 991
<210>52
<211>287
<212>PRT
<213>谷氨酸棒状杆菌
<400>52
Met Thr Leu Arg Cys Ser Asp Val Asn Val Glu Pro Leu Pro Gly Thr
1 5 10 15
Ala Lys Thr Gly Ser Gly Phe Val Leu Leu Glu His Ala Gly Ser Trp
20 25 30
Ser Arg Asp Val Leu Asp Gly Gly Thr Phe Asp Pro Glu Leu Thr Asp
35 40 45
Gln Leu Lys Arg His Leu Lys Ala Ser Gly Met Gly Leu Gln Leu Ile
50 55 60
Arg Lys Pro Gly Arg Glu Gly Arg Asn Val Glu Lys His Asn Leu Phe
65 70 75 80
Leu Val Phe Ala Glu Ala Ser Ile Ile Glu His Leu Val Val Asp Ala
85 90 95
Pro Ala Asp Val Leu Asp Leu Asp Leu Ser Gly Pro Gly Lys Asn Asn
100 105 110
Ala Gln Arg Met Asp Asp Pro Met Leu Leu Ile Cys Thr His Ser Lys
115 120 125
Arg Asp Val Cys Cys Ala Ile Lys Gly Arg Pro Leu Ala Ala Ala Val
130 135 140
Glu Pro Gln Phe Gly Pro Leu His Val Trp Glu Ala Ser His Thr Lys
145 150 155 160
Gly His Arg Phe Ala Pro Ser Met Leu Leu Met Pro Trp Asn Tyr Ser
165 170 175
Tyr Gly Leu Leu Asp Glu Ala Glu Thr Val Gln Leu Phe Gln Gly Ala
180 185 190
Leu Asp Asn Lys Leu Phe Leu Pro Gly Asn Arg Gly Arg Gly Thr Leu
195 200 205
Asp Ala Arg Gly Gln Val Ala Glu Ile Ala Val Ala Glu Ala Phe Gly
210 215 220
Glu Ala Val Ala Pro Ala Ser Leu Gln Val Glu Phe Glu Asp Asp Ser
225 230 235 240
Val Leu Val Thr His Pro Asp Gly Arg Thr Trp Val Val Glu Leu Glu
245 250 255
Arg Ile Glu Val Asp Gly Val Val Ser Ser Cys Gly Asp Gln Pro Lys
260 265 270
Thr Gly Lys Ala Trp Val Ala Arg Gln Val Thr Glu Leu Ile Gly
275 280 285
<210>53
<211>1039
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1009)
<223>RXA00486
<400>53
gtttattcat gcccttgatt attgccaaag aaacctttaa ggactagatc gaaaaacagc 60
caactatagt taagtaatac tgaacaattt tggaggtgtc gtg ctc aat ctc aac 115
Val Leu Asn Leu Asn
1 5
cgc tta cac atc ctg cag gaa ttc cac cgc ctg gga acg att aca gca 163
Arg Leu His Ile Leu Gln Glu Phe His Arg Leu Gly Thr Ile Thr Ala
10 15 20
gtg gcg gaa tcc atg sac tac agc cgc tct gcc atc tcc caa caa atg 211
Val Ala Glu Ser Met Asn Tyr Ser Arg Ser Ala Ile Ser Gln Gln Met
25 30 35
gcg ctg ctg gaa aaa gaa att ggt gtg aaa ctc ttt gaa aaa agc ggc 259
Ala Leu Leu Glu Lys Glu Ile Gly Val Lys Leu Phe Glu Lys Ser Gly
40 45 50
cga aac ctc tac ttc aca gaa caa ggc gaa gtg ttg gcc tca gaa aca 307
Arg Asn Leu Tyr Phe Thr Glu Gln Gly Glu Val Leu Ala Ser Glu Thr
55 60 65
cat gcg atc atg gca gca gtc gac cat gcc cgc gca gcc gtt cta gat 355
His Ala Ile Met Ala Ala Val Asp His Ala Arg Ala Ala Val Leu Asp
70 75 80 85
tcg ctg tct gaa gtg tcc gga acg ctg aaa gtc acc tcc ttc caa tcc 403
Ser Leu Ser Glu Val Ser Gly Thr Leu Lys Val Thr Ser Phe Gln Ser
90 95 100
ctg ctg ttc acc ctt gcc ccg aaa gcc atc gcg cgc ctg acc gag aaa 451
Leu Leu Phe Thr Leu Ala Pro Lys Ala Ile Ala Arg Leu Thr Glu Lys
105 110 115
tac cca cac ctg caa gta gaa atc tcc caa cta gaa gtc acc gca gcg 499
Tyr Pro His Leu Gln Val Glu Ile Ser Gln Leu Glu Val Thr Ala Ala
120 125 130
ctc gaa gaa ctc cgc gcc cgc cgc gtc gac gtc gca ctc ggc gag gaa 547
Leu Glu Glu Leu Arg Ala Arg Arg Val Asp Val Ala Leu Gly Glu Glu
135 140 145
tac ccc gtg gaa gtc ccc ctt gtt gag gcc agc att cac cgc gaa gtc 595
Tyr Pro Val Glu Val Pro Leu Val Glu Ala Ser Ile His Arg Glu Val
150 155 160 165
ctc ttc gaa gac ccc atg ctg ctc gtc acc cca gca agc ggc cca tac 643
Leu Phe Glu Asp Pro Met Leu Leu Val Thr Pro Ala Ser Gly Pro Tyr
170 175 180
tct ggc ctc acc ctg cca gaa ctc cgc gac atc ccc atc gcc atc gat 691
Ser Gly Leu Thr Leu Pro Glu Leu Arg Asp Ile Pro Ile Ala Ile Asp
185 190 195
cca ccc gac ctt ccc gcg ggc gaa tgg gtc cat agg ctc tgc cgg cgc 739
Pro Pro Asp Leu Pro Ala Gly Glu Trp Val His Arg Leu Cys Arg Arg
200 205 210
gcc ggg ttt gag ccc cgc gtg acc ttt gaa acc agc gat ccc atg ctc 787
Ala Gly Phe Glu Pro Arg Val Thr Phe Glu Thr Ser Asp Pro Met Leu
215 220 225
caa gca cac ctc gtg cgt agc ggc ttg gcc gtg aca ttt tcc ccc aca 835
Gln Ala His Leu Val Arg Ser Gly Leu Ala Val Thr Phe Ser Pro Thr
230 235 240 245
ctg ctc acc ccg atg ctg gaa agc gtg cac atc cag ccg ctg ccc ggc 883
Leu Leu Thr Pro Met Leu Glu Ser Val His Ile Gln Pro Leu Pro Gly
250 255 260
aac ccc acg cgc acg ctc tac acc gcg gtc agg gaa ggg cgc cag ggg 931
Asn Pro Thr Arg Thr Leu Tyr Thr Ala Val Arg Glu Gly Arg Gln Gly
265 270 275
cat cca gcc att aaa gct ttt cga cga gcc ctc gcc cat gtg gcc aaa 979
His Pro Ala Ile Lys Ala Phe Arg Arg Ala Leu Ala His Val Ala Lys
280 285 290
gaa tct tat ttg gag gct cgt cta gta gag tgagttcttg tgagccttca 1029
Glu Ser Tyr Leu Glu Ala Arg Leu Val Glu
295 300
gacaaatcat 1039
<210>54
<211>303
<212>PRT
<213>谷氨酸棒状杆菌
<400>54
Val Leu Asn Leu Asn Arg Leu His Ile Leu Gln Glu Phe His Arg Leu
1 5 10 15
Gly Thr Ile Thr Ala Val Ala Glu Ser Met Asn Tyr Ser Arg Ser Ala
20 25 30
Ile Ser Gln Gln Met Ala Leu Leu Glu Lys Glu Ile Gly Val Lys Leu
35 40 45
Phe Glu Lys Ser Gly Arg Asn Leu Tyr Phe Thr Glu Gln Gly Glu Val
50 55 60
Leu Ala Ser Glu Thr His Ala Ile Met Ala Ala Val Asp His Ala Arg
65 70 75 80
Ala Ala Val Leu Asp Ser Leu Ser Glu Val Ser Gly Thr Leu Lys Val
85 90 95
Thr Ser Phe Gln Ser Leu Leu Phe Thr Leu Ala Pro Lys Ala Ile Ala
100 105 110
Arg Leu Thr Glu Lys Tyr Pro His Leu Gln Val Glu Ile Ser Gln Leu
115 120 125
Glu Val Thr Ala Ala Leu Glu Glu Leu Arg Ala Arg Arg Val Asp Val
130 135 140
Ala Leu Gly Glu Glu Tyr Pro Val Glu Val Pro Leu Val Glu Ala Ser
145 150 155 160
Ile His Arg Glu Val Leu Phe Glu Asp Pro Met Leu Leu Val Thr Pro
165 170 175
Ala Ser Gly Pro Tyr Ser Gly Leu Thr Leu Pro Glu Leu Arg Asp Ile
180 185 190
Pro Ile Ala Ile Asp Pro Pro Asp Leu Pro Ala Gly Glu Trp Val His
195 200 205
Arg Leu Cys Arg Arg Ala Gly Phe Glu Pro Arg Val Thr Phe Glu Thr
210 215 220
Ser Asp Pro Met Leu Gln Ala His Leu Val Arg Ser Gly Leu Ala Val
225 230 235 240
Thr Phe Ser Pro Thr Leu Leu Thr Pro Met Leu Glu Ser Val His Ile
245 250 255
Gln Pro Leu Pro Gly Asn Pro Thr Arg Thr Leu Tyr Thr Ala Val Arg
260 265 270
Glu Gly Arg Gln Gly His Pro Ala Ile Lys Ala Phe Arg Arg Ala Leu
275 280 285
Ala His Val Ala Lys Glu Ser Tyr Leu Glu Ala Arg Leu Val Glu
290 295 300
<210>55
<211>1744
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1714)
<223>RXA00493
<400>55
cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60
gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115
Met Ala Lys Leu Ile
1 5
gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163
Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu Arg Gly Val Asp Ala
10 15 20
ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211
Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val
25 30 35
gtt ctt gat aag gca ttc ggc gga cct ctg gtc acc aac gac ggt gtc 259
Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val
40 45 50
acc att gcc cgc gac atc gac ctt gag gat cct ttt gag aac ctc ggt 307
Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly
55 60 65
gcg cag ctg gtg aag tcc gtt gct gtt aag acc aac gac atc gct ggt 355
Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr Asn Asp Ile Ala Gly
70 75 80 85
gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403
Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln Ala Leu Ile Ala Glu
90 95 100
ggc ctg cgc aac gtt gct gct ggc gca aac cca atg gag ctc aac aag 451
Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro Met Glu Leu Asn Lys
105 110 115
ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499
Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu Glu Leu Lys Ala Arg
120 125 130
gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547
Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala Asn Val Ala Thr Val
135 140 145
tca tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gca gcg atg gaa 595
Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val Ala Ala Ala Met Glu
150 155 160 165
aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643
Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser Ile
170 175 180
gag act gct ctc gag gtc acc gaa ggt att tct ttc gac aag ggc tac 691
Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser Phe Asp Lys Gly Tyr
185 190 195
ctt tcc cct tat ttc atc aac gac aac gac act cag cag gct gtc ctg 739
Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr Gln Gln Ala Val Leu
200 205 210
gac aac cct gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787
Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys Ile Ser Ser Leu Pro
215 220 225
gac ttc ctc cca ttg ctg gag aag gtt gtg gag tcc aac cgt cct ttg 835
Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu
230 235 240 245
ctg atc atc gca gaa gac gtc gag ggc gag cct ttg cag acc ctg gtt 883
Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val
250 255 260
gtg aac tcc atc cgc aag acc atc aag gtc gtt gca gtg aag tcc cct 931
Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val Ala Val Lys Ser Pro
265 270 275
tac ttc ggt gac cga cgc aag gcg ttc atg gat gac ctg gct att gtc 979
Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp Asp Leu Ala Ile Val
280 285 290
acc aag gca act gtc gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027
Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly Ile Asn Leu Asn Glu
295 300 305
gct ggc gaa gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075
Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg Ile Thr Val Ser Lys
310 315 320 325
gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123
Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser Ala Glu Asp Val Glu
330 335 340
gca cgt cgc ggc cag atc cgt cgc gaa atc gcc aac acc gat tcc acc 1171
Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala Asn Thr Asp Ser Thr
345 350 355
tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219
Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala Lys Leu Ser Gly Gly
360 365 370
att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267
Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu Thr Glu Val Asn Asp
375 380 385
cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315
Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala Ala Arg Ala Ala Ala
390 395 400 405
caa gaa ggc gtt atc gct ggt ggc ggt tcc gct ttg gtt cag atc gct 1363
Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala Leu Val Gln Ile Ala
410 415 420
gag act ctg aag gct tac gcc gaa gag ttc gaa ggc gac cag aag gtc 1411
Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu Gly Asp Gln Lys Val
425 430 435
ggc gtt cgc gca ctg gct act gct ttg ggc aag cca gcg tac tgg atc 1459
Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys Pro Ala Tyr Trp Ile
440 445 450
gcc tcc aac gca ggt ctt gac ggc tct gtt gtt gtt gca cgc act gct 1507
Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val Val Ala Arg Thr Ala
455 460 465
gct ctg cca aac ggc gag ggc ttc aac gct gca act ttg gaa tac gga 1555
Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala Thr Leu Glu Tyr Gly
470 475 480 485
aac ctg atc aac gac ggt gtc atc gac cca gtc aag gtc acc cat tcc 1603
Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val Lys Val Thr His Ser
490 495 500
gca gta gtg aat gca acc tct gtt gca cgc atg gtt ctg acc act gag 1651
Ala Val Val Asn Ala Thr Ser Val Ala Arg Met Val Leu Thr Thr Glu
505 510 515
gct tct gtt gtt gag aag cct gca gaa gaa gca gcc gat gca cat gca 1699
Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala Ala Asp Ala His Ala
520 525 530
gga cat cat cac cac taaagttctg tgaaaaacac cgtggggcag 1744
Gly His His His His
535
<210>56
<211>538
<212>PRT
<213>谷氨酸棒状杆菌
<400>56
Met Ala Lys Leu Ile Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu
1 5 10 15
Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro
20 25 30
Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val
35 40 45
Thr Asn Asp Gly Val Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro
50 55 60
Phe Glu Asn Leu Gly Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr
65 70 75 80
Asn Asp Ile Ala Gly Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln
85 90 95
Ala Leu Ile Ala Glu Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro
100 105 110
Met Glu Leu Asn Lys Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu
115 120 125
Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala
130 135 140
Asn Val Ala Thr Val Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val
145 150 155 160
Ala Ala Ala Met Glu Lys Val Gly Lys Asp Gly Val Val Thr Val Glu
165 170 175
Glu Ser Gln Ser Ile Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser
180 185 190
Phe Asp Lys Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr
195 200 205
Gln Gln Ala Val Leu Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys
210 215 220
Ile Ser Ser Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu
225 230 235 240
Ser Asn Arg Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro
245 250 255
Leu Gln Thr Leu Val Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val
260 265 270
Ala Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp
275 280 285
Asp Leu Ala Ile Val Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly
290 295 300
Ile Asn Leu Asn Glu Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg
305 310 315 320
Ile Thr Val Ser Lys Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser
325 330 335
Ala Glu Asp Val Glu Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala
340 345 350
Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala
355 360 365
Lys Leu Ser Gly Gly Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu
370 375 380
Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala
385 390 395 400
Ala Arg Ala Ala Ala Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala
405 410 415
Leu Val Gln Ile Ala Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu
420 425 430
Gly Asp Gln Lys Val Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys
435 440 445
Pro Ala Tyr Trp Ile Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val
450 455 460
Val Ala Arg Thr Ala Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala
465 470 475 480
Thr Leu Glu Tyr Gly Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val
485 490 495
Lys Val Thr His Ser Ala Val Val Asn Ala Thr Ser Val Ala Arg Met
500 505 510
Val Leu Thr Thr Glu Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala
515 520 525
Ala Asp Ala His Ala Gly His His His His
530 535
<210>57
<211>1156
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1126)
<223>RXA00496
<400>57
ctgcagagat ggtgggcagc accccaggtg ctgtacgagt tgcccaacac agggcactca 60
cgacacttcg aagcacactt gagcagcagg agaacaagta atg act cga cgt cta 115
Met Thr Arg Arg Leu
1 5
cat ggt ggt gag cag gat ggc cag gaa cac gtt aaa gga cag cta aag 163
His Gly Gly Glu Gln Asp Gly Gln Glu His Val Lys Gly Gln Leu Lys
10 15 20
cag ctg ttc gac gac gac gcg ttc ttg act gac ctg tcc cgc ggc gtt 211
Gln Leu Phe Asp Asp Asp Ala Phe Leu Thr Asp Leu Ser Arg Gly Val
25 30 35
gat ccc tca gag ggc gat gac gcc ctc gct ggc ctc ctc ctc gat tta 259
Asp Pro Ser Glu Gly Asp Asp Ala Leu Ala Gly Leu Leu Leu Asp Leu
40 45 50
aca aag gaa gct cag gag ccg ccg gca aca atg ccg gat tgg tct act 307
Thr Lys Glu Ala Gln Glu Pro Pro Ala Thr Met Pro Asp Trp Ser Thr
55 60 65
ttg ctc cct gga att ttg gat cag gat cag gat ttg cca gtg gaa tcc 355
Leu Leu Pro Gly Ile Leu Asp Gln Asp Gln Asp Leu Pro Val Glu Ser
70 75 80 85
act tcg gac acc acg gtt atg cag gca tca aac cct gca acc caa gaa 403
Thr Ser Asp Thr Thr Val Met Gln Ala Ser Asn Pro Ala Thr Gln Glu
90 95 100
ttc gca cct gtt tct att tct gat acc ccc aac act gca act aat tca 451
Phe Ala Pro Val Ser Ile Ser Asp Thr Pro Asn Thr Ala Thr Asn Ser
105 110 115
gct gat gca gat gag tcc gca act gtt gtt cca ctt gca gca cgc cgt 499
Ala Asp Ala Asp Glu Ser Ala Thr Val Val Pro Leu Ala Ala Arg Arg
120 125 130
gag aag cgt gcc aag agc gga tca agc ggg gtt cat tca ctg gat gct 547
Glu Lys Arg Ala Lys Ser Gly Ser Ser Gly Val His Ser Leu Asp Ala
135 140 145
tcg gca acc cag cgc aaa tct cac cca ttc ctt agc ggt ttg gtg ggt 595
Ser Ala Thr Gln Arg Lys Ser His Pro Phe Leu Ser Gly Leu Val Gly
150 155 160 165
gct gca gct gca act cta gtc atc gca ggc ggt gga gca gca gtg tac 643
Ala Ala Ala Ala Thr Leu Val Ile Ala Gly Gly Gly Ala Ala Val Tyr
170 175 180
aac gct gat gaa aac tcc ccg ttg tat ggc atg aat cag cag ctg ttt 691
Asn Ala Asp Glu Asn Ser Pro Leu Tyr Gly Met Asn Gln Gln Leu Phe
185 190 195
ggc aat caa gat tct cca agc gtg gtg gag ctt gcc tcc acg ctg gaa 739
Gly Asn Gln Asp Ser Pro Ser Val Val Glu Leu Ala Ser Thr Leu Glu
200 205 210
gaa gtt gat agt cgt aca gct agt ggc gat gtg gaa ggg gca cgt gct 787
Glu Val Asp Ser Arg Thr Ala Ser Gly Asp Val Glu Gly Ala Arg Ala
215 220 225
cta ctc gag cag gct cga gca atg ctg gat ggc atg gca cct cct cga 835
Leu Leu Glu Gln Ala Arg Ala Met Leu Asp Gly Met Ala Pro Pro Arg
230 235 240 245
aag gcg ccg tcg gag gca acc cga acg gtt gaa tct gaa cca ggt act 883
Lys Ala Pro Ser Glu Ala Thr Arg Thr Val Glu Ser Glu Pro Gly Thr
250 255 260
cag acg ttg act gca acg gtt act gaa tcc gca agt ccg gaa cca ccg 931
Gln Thr Leu Thr Ala Thr Val Thr Glu Ser Ala Ser Pro Glu Pro Pro
265 270 275
gtc acg gaa act caa act gtt acc tcc acc gag gta cag aca gtg aca 979
Val Thr Glu Thr Gln Thr Val Thr Ser Thr Glu Val Gln Thr Val Thr
280 285 290
acc act gcg gtt gct cca ccg gtc tgg act cct aat cca gag cca aca 1027
Thr Thr Ala Val Ala Pro Pro Val Trp Thr Pro Asn Pro Glu Pro Thr
295 300 305
acc aca gct gcc ccg act tct acg cct tca act ggt ggc ggt gag gga 1075
Thr Thr Ala Ala Pro Thr Ser Thr Pro Ser Thr Gly Gly Gly Glu Gly
310 315 320 325
acc ggc aat gat ggt gac tct gga ctt gtg cca cct cag act cct gga 1123
Thr Gly Asn Asp Gly Asp Ser Gly Leu Val Pro Pro Gln Thr Pro Gly
330 335 340
aac taggtaaaaa tataaaaact gctagcaacg 1156
Asn
<210>58
<211>342
<212>PRT
<213>谷氨酸棒状杆菌
<400>58
Met Thr Arg Arg Leu His Gly Gly Glu Gln Asp Gly Gln Glu His Val
1 5 10 15
Lys Gly Gln Leu Lys Gln Leu Phe Asp Asp Asp Ala Phe Leu Thr Asp
20 25 30
Leu Ser Arg Gly Val Asp Pro Ser Glu Gly Asp Asp Ala Leu Ala Gly
35 40 45
Leu Leu Leu Asp Leu Thr Lys Glu Ala Gln Glu Pro Pro Ala Thr Met
50 55 60
Pro Asp Trp Ser Thr Leu Leu Pro Gly Ile Leu Asp Gln Asp Gln Asp
65 70 75 80
Leu Pro Val Glu Ser Thr Ser Asp Thr Thr Val Met Gln Ala Ser Asn
85 90 95
Pro Ala Thr Gln Glu Phe Ala Pro Val Ser Ile Ser Asp Thr Pro Asn
100 105 110
Thr Ala Thr Asn Ser Ala Asp Ala Asp Glu Ser Ala Thr Val Val Pro
115 120 125
Leu Ala Ala Arg Arg Glu Lys Arg Ala Lys Ser Gly Ser Ser Gly Val
130 135 140
His Ser Leu Asp Ala Ser Ala Thr Gln Arg Lys Ser His Pro Phe Leu
145 150 155 160
Ser Gly Leu Val Gly Ala Ala Ala Ala Thr Leu Val Ile Ala Gly Gly
165 170 175
Gly Ala Ala Val Tyr Asn Ala Asp Glu Asn Ser Pro Leu Tyr Gly Met
180 185 190
Asn Gln Gln Leu Phe Gly Asn Gln Asp Ser Pro Ser Val Val Glu Leu
195 200 205
Ala Ser Thr Leu Glu Glu Val Asp Ser Arg Thr Ala Ser Gly Asp Val
210 215 220
Glu Gly Ala Arg Ala Leu Leu Glu Gln Ala Arg Ala Met Leu Asp Gly
225 230 235 240
Met Ala Pro Pro Arg Lys Ala Pro Ser Glu Ala Thr Arg Thr Val Glu
245 250 255
Ser Glu Pro Gly Thr Gln Thr Leu Thr Ala Thr Val Thr Glu Ser Ala
260 265 270
Ser Pro Glu Pro Pro Val Thr Glu Thr Gln Thr Val Thr Ser Thr Glu
275 280 285
Val Gln Thr Val Thr Thr Thr Ala Val Ala Pro Pro Val Trp Thr Pro
290 295 300
Asn Pro Glu Pro Thr Thr Thr Ala Ala Pro Thr Ser Thr Pro Ser Thr
305 310 315 320
Gly Gly Gly Glu Gly Thr Gly Asn Asp Gly Asp Ser Gly Leu Val Pro
325 330 335
Pro Gln Thr Pro Gly Asn
340
<210>59
<211>1852
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1822)
<223>RXA00503
<400>59
aatccacgaa ggtgcccaag aatcaccaat ttcggtcgga actgatcctt ctttgtccgt 60
tgagacccca caggtgtttt aaacatttag tattagttcc atg aaa cca gtc ttc 115
Met Lys Pro Val Phe
1 5
tcc gtt gac caa atc cga cgc gca gaa aac acc ctt ttt gag ctt cag 163
Ser Val Asp Gln Ile Arg Arg Ala Glu Asn Thr Leu Phe Glu Leu Gln
10 15 20
gca gat ccg gat gag ctg atg atc tcc gcg gca tcg gcg gtg gcc gat 211
Ala Asp Pro Asp Glu Leu Met Ile Ser Ala Ala Ser Ala Val Ala Asp
25 30 35
gtc gcg ttg gca atg gtg gac ggc ccc gct cca gcg gtc tcc agt gag 259
Val Ala Leu Ala Met Val Asp Gly Pro Ala Pro Ala Val Ser Ser Glu
40 45 50
gag tca atc ctg ctg ctg gtc ggc ccc ggt ggc aac ggt ggc gac gcc 307
Glu Ser Ile Leu Leu Leu Val Gly Pro Gly Gly Asn Gly Gly Asp Ala
55 60 65
ttg tat gca ggc gcg ttc ctt gca gaa gaa ggc cac cac gtt gat gct 355
Leu Tyr Ala Gly Ala Phe Leu Ala Glu Glu Gly His His Val Asp Ala
70 75 80 85
ttg ctg ttg gga aac ggc aaa gtc cat caa tca gca ttg gca tat tat 403
Leu Leu Leu Gly Asn Gly Lys Val His Gln Ser Ala Leu Ala Tyr Tyr
90 95 100
gag tct ttg ggc ggg cag atc att tcc gat ttt ccc cct cac tac ctc 451
Glu Ser Leu Gly Gly Gln Ile Ile Ser Asp Phe Pro Pro His Tyr Leu
105 110 115
tac cgc ctg gtg att gat ggt ttg ttt ggc atc ggt ggt cgg gga ggg 499
Tyr Arg Leu Val Ile Asp Gly Leu Phe Gly Ile Gly Gly Arg Gly Gly
120 125 130
ctc acc cca gag ctg gcc agt ttg gtg gag tct ttt tcc gct tca ggt 547
Leu Thr Pro Glu Leu Ala Ser Leu Val Glu Ser Phe Ser Ala Ser Gly
135 140 145
atc ccc att ttg gcg att gat gtg ccg tct ggc gtg cat gcc gat agt 595
Ile Pro Ile Leu Ala Ile Asp Val Pro Ser Gly Val His Ala Asp Ser
150 155 160 165
ggt gaa ctg ccg ccc ggc gtg atg gtg acg gtg gaa gga ttt gat aat 643
Gly Glu Leu Pro Pro Gly Val Met Val Thr Val Glu Gly Phe Asp Asn
170 175 180
gat gca ccg atg gcg cgt cag aaa att ccg gca cac att gac gct gat 691
Asp Ala Pro Met Ala Arg Gln Lys Ile Pro Ala His Ile Asp Ala Asp
185 190 195
gtc acg atc acg ttt ggc ggt ttg aga cgc gcc cac gcg gtc agt cct 739
Val Thr Ile Thr Phe Gly Gly Leu Arg Arg Ala His Ala Val Ser Pro
200 205 210
gcg tgt ggt gaa gtg ctc tgt gct gat atc aac atc gct ggt ggc ggc 787
Ala Cys Gly Glu Val Leu Cys Ala Asp Ile Asn Ile Ala Gly Gly Gly
215 220 225
gga aaa tcg ctg tcc gct gag ttg agt cag gtg cag gca gaa gac gcg 835
Gly Lys Ser Leu Ser Ala Glu Leu Ser Gln Val Gln Ala Glu Asp Ala
230 235 240 245
acc ccg cag atg ttt gcc tcc aag gcg tat caa cgg aaa gat tcg ctt 883
Thr Pro Gln Met Phe Ala Ser Lys Ala Tyr Gln Arg Lys Asp Ser Leu
250 255 260
ttt gag cgc gcg aat ctc aaa gct acg gcg cca cat atc cat agg atc 931
Phe Glu Arg Ala Asn Leu Lys Ala Thr Ala Pro His Ile His Arg Ile
265 270 275
ggc cag cac ttt acc gtg ttg aac atg gag cct ggc ccg gat cat gat 979
Gly Gln His Phe Thr Val Leu Asn Met Glu Pro Gly Pro Asp His Asp
280 285 290
aaa tac agt ggc gga att gtc ggc att gtt gca ggt agt ggc acc tat 1027
Lys Tyr Ser Gly Gly Ile Val Gly Ile Val Ala Gly Ser Gly Thr Tyr
295 300 305
cca ggt gct gct gtg ctg tcg gtg aag gcg gct gtc agg gcc aca agc 1075
Pro Gly Ala Ala Val Leu Ser Val Lys Ala Ala Val Arg Ala Thr Ser
310 315 320 325
gcc atg gtt cga tac gtt ggc cct gcg tta aat ttt gtc atc cag tcg 1123
Ala Met Val Arg Tyr Val Gly Pro Ala Leu Asn Phe Val Ile Gln Ser
330 335 340
ctg ccg gag gtc gtc gca acg caa tca ctt gcc acc gcc ggc cgc gtg 1171
Leu Pro Glu Val Val Ala Thr Gln Ser Leu Ala Thr Ala Gly Arg Val
345 350 355
caa gcg tgg gtg cac ggc ccc gga cgc ggg ctg gag gct gag caa tca 1219
Gln Ala Trp Val His Gly Pro Gly Arg Gly Leu Glu Ala Glu Gln Ser
360 365 370
gcc gag ctt gcg gag ctt ttg agc cgg cct gag cct gtg ctt atc gac 1267
Ala Glu Leu Ala Glu Leu Leu Ser Arg Pro Glu Pro Val Leu Ile Asp
375 380 385
gcc gac agc ctc tca tta ctc cag ctc tca gcg gag ctt cgg cag gcg 1315
Ala Asp Ser Leu Ser Leu Leu Gln Leu Ser Ala Glu Leu Arg Gln Ala
390 395 400 405
ttg cgc gag cga aaa gca cca acg gtg ctc act ccg cac aag ggc gaa 1363
Leu Arg Glu Arg Lys Ala Pro Thr Val Leu Thr Pro His Lys Gly Glu
410 415 420
ttt gaa cgc atc gca gca gaa tta cgc tct gaa ggc gtc gag att ccc 1411
Phe Glu Arg Ile Ala Ala Glu Leu Arg Ser Glu Gly Val Glu Ile pro
425 430 435
caa gcg gac aaa gat ccc att ggt gct gcg caa gcg tta gct aaa gaa 1459
Gln Ala Asp Lys Asp Pro Ile Gly Ala Ala Gln Ala Leu Ala Lys Glu
440 445 450
ttt gat tgt tgc gta ctg ctc aag ggg aaa tac acc gtc attg ca gct 1507
Phe Asp Cys Cys Val Leu Leu Lys Gly Lys Tyr Thr Val Ile Ala Ala
455 460 465
cac gac ttt gtg cat gcg atc aac gct ggg cat tcc tgg ttg gct aca 1555
His Asp Phe Val His Ala Ile Asn Ala Gly His Ser Trp Leu Ala Thr
470 475 480 485
cct ggc tct ggc gat gtg ttg tca ggt ctt gtc ggt gca cac ttg gct 1603
Pro Gly Ser Gly Asp Val Leu Ser Gly Leu Val Gly Ala His Leu Ala
490 495 500
caa agc tac gca gaa tta aac cgc ttg ccg gag ttt ttc ccc gat gtg 1651
Gln Ser Tyr Ala Glu Leu Asn Arg Leu Pro Glu Phe Phe Pro Asp Val
505 510 515
acc ttg tct gat tcg gcg att tac acc cag att gca cct gct gcg acc 1699
Thr Leu Ser Asp Ser Ala Ile Tyr Thr Gln Ile Ala Pro Ala Ala Thr
520 525 530
atc cac gcg gtt gct gct ggg ttg gcc gca cga acc gaa ttt ggg ttt 1747
Ile His Ala Val Ala Ala Gly Leu Ala Ala Arg Thr Glu Phe Gly Phe
535 540 545
gcg ccg acc tcc gca agt ttg atc gct gat gcc atc cct gca gcg acc 1795
Ala Pro Thr Ser Ala Ser Leu Ile Ala Asp Ala Ile Pro Ala Ala Thr
550 555 560 565
gcc aag gtg gat ttg aag cga att gtc tagctctgca tgaattccgt 1842
Ala Lys Val Asp Leu Lys Arg Ile Val
570
atgaattctt 1852
<210>60
<211>574
<212>PRT
<213>谷氨酸棒状杆菌
<400>60
Met Lys Pro Val Phe Ser Val Asp Gln Ile Arg Arg Ala Glu Asn Thr
1 5 10 15
Leu Phe Glu Leu Gln Ala Asp Pro Asp Glu Leu Met Ile Ser Ala Ala
20 25 30
Ser Ala Val Ala Asp Val Ala Leu Ala Met Val Asp Gly Pro Ala Pro
35 40 45
Ala Val Ser Ser Glu Glu Ser Ile Leu Leu Leu Val Gly Pro Gly Gly
50 55 60
Asn Gly Gly Asp Ala Leu Tyr Ala Gly Ala Phe Leu Ala Glu Glu Gly
65 70 75 80
His His Val Asp Ala Leu Leu Leu Gly Asn Gly Lys Val His Gln Ser
85 90 95
Ala Leu Ala Tyr Tyr Glu Ser Leu Gly Gly Gln Ile Ile Ser Asp Phe
100 105 110
Pro Pro His Tyr Leu Tyr Arg Leu Val Ile Asp Gly Leu Phe Gly Ile
115 120 125
Gly Gly Arg Gly Gly Leu Thr Pro Glu Leu Ala Ser Leu Val Glu Ser
130 135 140
Phe Ser Ala Ser Gly Ile Pro Ile Leu Ala Ile Asp Val Pro Ser Gly
145 150 155 160
Val His Ala Asp Ser Gly Glu Leu Pro Pro Gly Val Met Val Thr Val
165 170 175
Glu Gly Phe Asp Asn Asp Ala Pro Met Ala Arg Gln Lys Ile Pro Ala
180 185 190
His Ile Asp Ala Asp Val Thr Ile Thr Phe Gly Gly Leu Arg Arg Ala
195 200 205
His Ala Val Ser Pro Ala Cys Gly Glu Val Leu Cys Ala Asp Ile Asn
210 215 220
Ile Ala Gly Gly Gly Gly Lys Ser Leu Ser Ala Glu Leu Ser Gln Val
225 230 235 240
Gln Ala Glu Asp Ala Thr Pro Gln Met Phe Ala Ser Lys Ala Tyr Gln
245 250 255
Arg Lys Asp Ser Leu Phe Glu Arg Ala Asn Leu Lys Ala Thr Ala Pro
260 265 270
His Ile His Arg Ile Gly Gln His Phe Thr Val Leu Asn Met Glu Pro
275 280 285
Gly Pro Asp His Asp Lys Tyr Ser Gly Gly Ile Val Gly Ile Val Ala
290 295 300
Gly Ser Gly Thr Tyr Pro Gly Ala Ala Val Leu Ser Val Lys Ala Ala
305 310 315 320
Val Arg Ala Thr Ser Ala Met Val Arg Tyr Val Gly Pro Ala Leu Asn
325 330 335
Phe Val Ile Gln Ser Leu Pro Glu Val Val Ala Thr Gln Ser Leu Ala
340 345 350
Thr Ala Gly Arg Val Gln Ala Trp Val His Gly Pro Gly Arg Gly Leu
355 360 365
Glu Ala Glu Gln Ser Ala Glu Leu Ala Glu Leu Leu Ser Arg Pro Glu
370 375 380
Pro Val Leu Ile Asp Ala Asp Ser Leu Ser Leu Leu Gln Leu Ser Ala
385 390 395 400
Glu Leu Arg Gln Ala Leu Arg Glu Arg Lys Ala Pro Thr Val Leu Thr
405 410 415
Pro His Lys Gly Glu Phe Glu Arg Ile Ala Ala Glu Leu Arg Ser Glu
420 425 430
Gly Val Glu Ile Pro Gln Ala Asp Lys Asp Pro Ile Gly Ala Ala Gln
435 440 445
Ala Leu Ala Lys Glu Phe Asp Cys Cys Val Leu Leu Lys Gly Lys Tyr
450 455 460
Thr Val Ile Ala Ala His Asp Phe Val His Ala Ile Asn Ala Gly His
465 470 475 480
Ser Trp Leu Ala Thr Pro Gly Ser Gly Asp Val Leu Ser Gly Leu Val
485 490 495
Gly Ala His Leu Ala Gln Ser Tyr Ala Glu Leu Asn Arg Leu Pro Glu
500 505 510
Phe Phe Pro Asp Val Thr Leu Ser Asp Ser Ala Ile Tyr Thr Gln Ile
515 520 525
Ala Pro Ala Ala Thr Ile His Ala Val Ala Ala Gly Leu Ala Ala Arg
530 535 540
Thr Glu Phe Gly Phe Ala Pro Thr Ser Ala Ser Leu Ile Ala Asp Ala
545 550 555 560
Ile Pro Ala Ala Thr Ala Lys Val Asp Leu Lys Arg Ile Val
565 570
<210>61
<211>985
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(955)
<223>RXA00507
<400>61
gttcatgttg aactattgtg ccaccaaccg cggacagaaa tacgattggt gccggggtta 60
gtgccagtat tgccccacgc tttcaactat ccttaaacac gtg gct gag aat ctg 115
Val Ala Glu Asn Leu
1 5
aac aaa cac ctg tcc aaa ctg tcc aag cgc gga ccg cac cgc gtg ctg 163
Asn Lys His Leu Ser Lys Leu Ser Lys Arg Gly Pro His Arg Val Leu
10 15 20
gta ggc gat atg aac tac gcc ggc atc ccg ggc aaa atc tac acc cca 211
Val Gly Asp Met Asn Tyr Ala Gly Ile Pro Gly Lys Ile Tyr Thr Pro
25 30 35
gca gaa ggc gac ggc atc cca ggt gta gct ttc ggc cac gac tgg atg 259
Ala Glu Gly Asp Gly Ile Pro Gly Val Ala Phe Gly His Asp Trp Met
40 45 50
aaa tcc atc aag tac tac cac caa act ttg cga cac ctc gcg tcc tgg 307
Lys Ser Ile Lyg Tyr Tyr His Gln Thr Leu Arg His Leu Ala Ser Trp
55 60 65
ggc atc gct gtt gcc gcc cca gac acc gaa aat ggc ttc atg cca gac 355
Gly Ile Ala Val Ala Ala Pro Asp Thr Glu Asn Gly Phe Met Pro Asp
70 75 80 85
cac aaa ggt ttc gcc tct gac ctc gaa tcc tcc att cag att ctc ggc 403
His Lys Gly Phe Ala Ser Asp Leu Glu Ser Ser Ile Gln Ile Leu Gly
90 95 100
ggc gta aaa ctc ggc tcc gga aac gtc acc gtc aac cca gcc tgc ctc 451
Gly Val Lys Leu Gly Ser Gly Asn Val Thr Val Asn Pro Ala Cys Leu
105 110 115
ggt gta gta ggc cac ggc atg ggt gctggg gct gca gta cta tcc gca 499
Gly Val Val Gly His Gly Met Gly Ala Gly Ala Ala Val Leu Ser Ala
120 125 130
gca aac cgc gac ctc gtg cgc gca gtc gga gca atc tac cca gcg aaa 547
Ala Asn Arg Asp Leu Val Arg Ala Val Gly Ala Ile Tyr Pro Ala Lys
135 140 145
acc tcc ccc tca gca atc gac gcc gcc ttc gct gtc aaa gcc cca ggc 595
Thr Ser Pro Ser Ala Ile Asp Ala Ala Phe Ala Val Lys Ala Pro Gly
150 155 160 165
cta gtc atc gga tcc tcc agc ctc ggc ctc ttt gaa tcc ggc gag cca 643
Leu Val Ile Gly Ser Ser Ser Leu Gly Leu Phe Glu Ser Gly Glu Pro
170 175 180
gca aaa ctc gca gcc aac tgg gcc ggc gat gtc tgc tac cgc gaa tca 691
Ala Lys Leu Ala Ala Asn Trp Ala Gly Asp Val Cys Tyr Arg Glu Ser
185 190 195
gaa aaa ggc aac caa cag ggc ttc tct gaa gac acc atg ttc aaa ctt 739
Glu Lys Gly Asr Gln Gln Gly Phe Ser Glu Asp Thr Met Phe Lys Leu
200 205 210
gtc gca gga atc ggc agc cca caa acc gga gct caa gaa acc gtc cgc 787
Val Ala Gly Ile Gly Ser Pro Gln Thr Gly Ala Gln G1u Thr Val Arg
215 220 225
ggc ctc ctc acc gga ttc ctt ctc cac caa ctt gcc gga gaa aag aaa 835
Gly Leu Leu Thr Gly Phe Leu Leu His Gln Leu Ala Gly Glu Lys Lys
230 235 240 245
tac aaa gca ttc tcc gaa cca gac gct gaa gct aag aaa gtt gtc tcc 883
Tyr Lys Ala Phe Ser Glu Pro Asp Ala Glu Ala Lys Lys Val Val Ser
250 255 260
tac ttc ggc cag gag ctg cag gaa cat gcc ttc cct aag gac acg tcc 931
Tyr Phe Gly Gln Glu Leu Gln Glu His Ala Phe Pro Lys Asp Thr Ser
265 270 275
cca ttc gcg ttc ctt aac gag aag tagttcgctt ttcttagtgg gtgattttgt 985
Pro Phe Ala Phe Leu Asn Glu Lys
280 285
<210>62
<211>285
<212>PRT
<213>谷氨酸棒状杆菌
<400>62
Val Ala Glu Asn Leu Asn Lys His Leu Ser Lys Leu Ser Lys Arg Gly
1 5 10 15
Pro His Arg Val Lau Val Gly Asp Met Asn Tyr Ala Gly Ile Pro Gly
20 25 30
Lys Ile Tyr Thr Pro Ala Glu Gly Asp Gly Ile Pro Gly Val Ala Phe
35 40 45
Gly His Asp Trp Met Lys Ser Ile Lys Tyr Tyr His Gln Thr Leu Arg
50 55 60
His Leu Ala Ser Trp Gly Ile Ala Val Ala Ala Pro Asp Thr Glu Asn
65 70 75 80
Gly Phe Met Pro Asp His Lys Gly Phe Ala Ser Asp Leu Glu Ser Ser
85 90 95
Ile Gln Ile Leu Gly Gly Val Lys Leu Gly Ser Gly Asn Val Thr Val
100 105 110
Asn Pro Ala Cys Leu Gly Val Val Gly His Gly Met Gly Ala Gly Ala
115 120 125
Ala Val Leu Ser Ala Ala Asn Arg Asp Leu Val Arg Ala Val Gly Ala
130 135 140
Ile Tyr Pro Ala Lys Thr Ser Pro Ser Ala Ile Asp Ala Ala Phe Ala
145 150 155 160
Val Lys Ala Pro Gly Leu Val Ile Gly Ser Ser Ser Leu Gly Leu Phe
165 170 175
Glu Ser Gly Glu Pro Ala Lys Leu Ala Ala Asn Trp Ala Gly Asp Val
180 185 190
Cys Tyr Arg Glu Ser Glu Lys Gly Asn Gln Gln Gly Phe Ser Glu Asp
195 200 205
Thr Met Phe Lys Leu Val Ala Gly Ile Gly Ser Pro Gln Thr Gly Ala
210 215 220
Gln Glu Thr Val Arg Gly Leu Leu Thr Gly Phe Leu Leu His Gln Leu
225 230 235 240
Ala Gly Glu Lys Lys Tyr Lys Ala Phe Ser Glu Pro Asp Ala Glu Ala
245 250 255
Lys Lys Val Val Ser Tyr Phe Gly Gln Glu Leu Gln Glu His Ala Phe
260 265 270
Pro Lys Asp Thr Ser Pro Phe Ala Phe Leu Asn Glu Lys
275 280 285
<210>63
<211>1639
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1609)
<223>RXA00510
<400>63
acgcttttac ccaaacaacc caagccgccg aggaagaagt taaacgattc gacaataccg 60
acaaagtttt tggtgccgga tttgatggga ttgatccgaa gtg aat gat cct tta 115
Val Asn Asp Pro Leu
1 5
gcc ggC tac gga gca gtg atc tcc gca cta caa ggc gcc agt ggt ggg 163
Ala Gly Tyr Gly Ala Val Ile Ser Ala Leu Gln Gly Ala Ser Gly Gly
10 15 20
atg tat cgc gga ccg gcg aaa tcc gaa ggc cag ttg cgc gag atg tac 211
Met Tyr Arg Gly Pro Ala Lys Ser Glu Gly Gln Leu Arg Glu Met Tyr
25 30 35
caa acc atc gaa ggg cta gac acc agc tcg ttg cgc gaa gct gcc gaa 259
Gln Thr Ile Glu Gly Leu Asp Thr Ser Ser Leu Arg Glu Ala Ala Glu
40 45 50
gca gca gtg ggc gga acg aac gaa gcc aga ata caa ggg tgg gtc ggc 307
Ala Ala Val Gly Gly Thr Asn Glu Ala Arg Ile Gln Gly Trp Val Gly
55 60 65
ccg ctc ttg aaa ttc ttc ggg acg gtt ggc ggg gga atg atc gcc acg 355
Pro Leu Leu Lys Phe Phe Gly Thr Val Gly Gly Gly Met Ile Ala Thr
70 75 80 85
gag ata gct gaa cgg gca gtc gat tgg ttc aaa aac cgt aat gat gtg 403
Glu Ile Ala Glu Arg Ala Val Asp Trp Phe Lys Asn Arg Asn Asp Val
90 95 100
gaa gaa gtc agc gaa gcc gct gat aaa gcc gcc gat gcg atc gac tcc 451
Glu Glu Val Ser Glu Ala Ala Asp Lys Ala Ala Asp Ala Ile Asp Ser
105 110 115
act gtc aca gag tcc gac cag ggc atg atg cac att atc cag cag ctc 499
Thr Val Thr Glu Ser Asp Gln Gly Met Met His Ile Ile Gln Gln Leu
120 125 130
ttg gac att gtg tct acg ttg acg cag att ctt ggc agc atg gat cgg 547
Leu Asp Ile Val Ser Thr Leu Thr Gln Ile Leu Gly Ser Met Asp Arg
135 140 145
ggg aaa ttt cct caa gaa ttc cgg gac tgt gtc caa act gga gct gat 595
Gly Lys Phe Pro Gln Glu Phe Arg Asp Cys Val Gln Thr Gly Ala Asp
150 155 160 165
ctt att gac cag gca ggg gac atg ctt gaa gga ttg tgc gct gat cga 643
Leu Ile Asp Gln Ala Gly Asp Met Leu Glu Gly Leu Cys Ala Asp Arg
170 175 180
gat gat gcg att tca cag tgt ttc tcc gcg ttg acc gat cat gga aaa 691
Asp Asp Ala Ile Ser Gln Cys Phe Ser Ala Leu Thr Asp His Gly Lys
185 190 195
caa gtt tgt gaa act gag cca aag ccg ttg tgt agt gca gct tct ggg 739
Gln Val Cys Glu Thr Glu Pro Lys Pro Leu Cys Ser Ala Ala Ser Gly
200 205 210
ggt tca tct ggt gga gcg act tct tcg gct gcg gcc tct tca ggt ggt 787
Gly Ser Ser Gly Gly Ala Thr Ser Ser Ala Ala Ala Ser Ser Gly Gly
215 220 225
tca agc tcg agc act gct agc tct ggg agt tct ggc gga tcg agc agt 835
Ser Ser Ser Ser Thr Ala Ser Ser Gly Ser Ser Gly Gly Ser Ser Ser
230 235 240 245
gct gcg gat agt tcg acg tcg acc aat gct gaa tcc agt gtt gaa aag 883
Ala Ala Asp Ser Ser Thr Ser Thr Asn Ala Glu Ser Ser Val Glu Lys
250 255 260
gaa aag acc aca cct gct gcc gtt gag aaa cct gac gag aaa cca gtg 931
Glu Lys Thr Thr Pro Ala Ala Val Glu Lys Pro Asp Glu Lys Pro Val
265 270 275
gag aag ccg gtt gaa aag acg cca gaa aag ccg gta gaa aaa cct gtc 979
Glu Lys Pro Val Glu Lys Thr Pro Glu Lys Pro Val Glu Lys Pro Val
280 285 290
gag aag caa gag tgt gaa gaa aag cct gac cct gac ccg gaa aaa tgc 1027
Glu Lys Gln Glu Cys Glu Glu Lys Pro Asp Pro Asp Pro Glu Lys Cys
295 300 305
aaa aca gag ccg gtc gag tgt gag cca aca ccg aaa cct gaa aca gag 1075
Lys Thr Glu Pro Val Glu Cys Glu Pro Thr Pro Lys Pro Glu Thr Glu
310 315 320 325
cct gaa cca aaa ccg aca ccc acg ccc aca ccc gga aca cca aca cca 1123
Pro Glu Pro Lys Pro Thr Pro Thr Pro Thr Pro Gly Thr Pro Thr Pro
330 335 340
ata cct gag cta gag act gag gat tgt gaa cct ggc aag gag acg ggc 1171
Ile Pro Glu Leu Glu Thr Glu Asp Cys Glu Pro Gly Lys Glu Thr Gly
345 350 355
aca gac tca gaa tct gaa tcg gaa gag tgc gcc ccc gag ctc aat gac 1219
Thr Asp Ser Glu Ser Glu Ser Glu Glu Cys Ala Pro Glu Leu Asn Asp
360 365 370
gtt cca gaa gag tcg gat ctg att ggg caa ctc atc aag gga gca atc 1267
Val Pro Glu Glu Ser Asp Leu Ile Gly Gln Leu Ile Lys Gly Ala Ile
375 380 385
ggt att ggg att gtt gtt gta ggc gtt ggg ctg ttg gtg aat ttc ctg 1315
Gly Ile Gly Ile Val Val Val Gly Val Gly Leu Leu Val Asn Phe Leu
390 395 400 405
gag cag tgc gtc cct gtg att gaa gaa gta cct gtg ccg gag ccc gag 1363
Glu Gln Cys Val Pro Val Ile Glu Glu Val Pro Val Pro Glu Pro Glu
410 415 420
cct ata cct gaa ccc gct ccg cag cct gag cca act tca gtg aaa cca 1411
Pro Ile Pro Glu Pro Ala Pro Gln Pro Glu Pro Thr Ser Val Lys Pro
425 430 435
cca gag tct gaa cta gat aag gtg gct gag cct gcg ccg aag cca att 1459
Pro Glu Ser Glu Leu Asp Lys Val Ala Glu Pro Ala Pro Lys Pro Ile
440 445 450
cca caa gct aat tac act gct gct gca gca act aac tat tcc gct ccc 1507
Pro Gln Ala Asn Tyr Thr Ala Ala Ala Ala Thr Asn Tyr Ser Ala Pro
455 460 465
gcg cac gct ccc gtt gtc cca att acg ccg gca gca cct gaa gtt ccg 1555
Ala His Ala Pro Val Val Pro Ile Thr Pro Ala Ala Pro Glu Val Pro
470 475 480 485
gca gca cca gaa gtg cct gca ccg gcg gtt aat crt cac aag gcc ggc 1603
Ala Ala Pro Glu Val Pro Ala Pro Ala Val Asn Leu His Lys Ala Gly
490 495 500
ggg tgg tagcggtgga gtatgcggaa tttctccagc 1639
Gly Trp
<210>64
<211>503
<212>PRT
<213>谷氨酸棒状杆菌
<400>64
Val Asn Asp Pro Leu Ala Gly Tyr Gly Ala Val Ile Ser Ala Leu Gln
1 5 10 15
Gly Ala Ser Gly Gly Met Tyr Arg Gly Pro Ala Lys Ser Glu Gly Gln
20 25 30
Leu Arg Glu Met Tyr Gln Thr Ile Glu Gly Leu Asp Thr Ser Ser Leu
35 40 45
Arg Glu Ala Ala Glu Ala Ala Val Gly Gly Thr Asn Glu Ala Arg Ile
50 55 60
Gln Gly Trp Val Gly Pro Leu Leu Lys Phe Phe Gly Thr Val Gly Gly
65 70 75 80
Gly Met Ile Ala Thr Glu Ile Ala Glu Arg Ala Val Asp Trp Phe Lys
85 90 95
Asn Arg Asn Asp Val Glu Glu Val Ser Glu Ala Ala Asp Lys Ala Ala
100 105 110
Asp Ala Ile Asp Ser Thr Val Thr Glu Ser Asp Gln Gly Met Met His
115 120 125
Ile Ile Gln Gln Leu Leu Asp Ile Val Ser Thr Leu Thr Gln Ile Leu
130 135 140
Gly Ser Met Asp Arg Gly Lys Phe Pro Gln Glu Phe Arg Asp Cys Val
145 150 155 160
Gln Thr Gly Ala Asp Leu Ile Asp Gln Ala Gly Asp Met Leu Glu Gly
165 170 175
Leu Cys Ala Asp Arg Asp Asp Ala Ile Ser Gln Cys Phe Ser Ala Leu
180 185 190
Thr Asp His Gly Lys Gln Val Cys Glu Thr Glu Pro Lys Pro Leu Cys
195 200 205
Ser Ala Ala Ser Gly Gly Ser Ser Gly Gly Ala Thr Ser Ser Ala Ala
210 215 220
Ala Ser Ser Gly Gly Ser Ser Ser Ser Thr Ala Ser Ser Gly Ser Ser
225 230 235 240
Gly Gly Ser Ser Ser Ala Ala Asp Ser Ser Thr Ser Thr Asn Ala Glu
245 250 255
Ser Ser Val Glu Lys Glu Lys Thr Thr Pro Ala Ala Val Glu Lys Pro
260 265 270
Asp Glu Lys Pro Val Glu Lys Pro Val Glu Lys Thr Pro Glu Lys Pro
275 280 285
Val Glu Lys Pro Val Glu Lys Gln Glu Cys Glu Glu Lys Pro Asp Pro
290 295 300
Asp Pro Glu Lys Cys Lys Thr Glu Pro Val Glu Cys Glu Pro Thr Pro
305 310 315 320
Lys Pro Glu Thr Glu Pro Glu Pro Lys Pro Thr Pro Thr Pro Thr Pro
325 330 335
Gly Thr Pro Thr Pro Ile Pro Glu Leu Glu Thr Glu Asp Cys Glu Pro
340 345 350
Gly Lys Glu Thr Gly Thr Asp Ser Glu Ser Glu Ser Glu Glu Cys Ala
355 360 365
Pro Glu Leu Asn Asp Val Pro Glu Glu Ser Asp Leu Ile Gly Gln Leu
370 375 380
Ile Lys Gly Ala Ile Gly Ile Gly Ile Val Val Val Gly Val Gly Leu
385 390 395 400
Leu Val Asn Phe Leu Glu Gln Cys Val Pro Val Ile Glu Glu Val Pro
405 4l0 415
Val Pro Glu Pro Glu Pro Ile Pro Glu Pro Ala Pro Gln Pro Glu Pro
420 425 430
Thr Ser Val Lys Pro Pro Glu Ser Glu Leu Asp Lys Val Ala Glu Pro
435 440 445
Ala Pro Lys Pro Ile Pro Gln Ala Asn Tyr Thr Ala Ala Ala Ala Thr
450 455 460
Asn Tyr Ser Ala Pro Ala His Ala Pro Val Val Pro Ile Thr Pro Ala
465 470 475 480
Ala Pro Glu Val Pro Ala Ala Pro Glu Val Pro Ala Pro Ala Val Asn
485 490 495
Leu His Lys Ala Gly Gly Trp
500
<210>65
<211>2290
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2260)
<223>RXA00550
<400>65
gccagctgat tctcagattc agatgaatag gcttaggcgc agtattctct acagcacaag 60
cttcagataa gaaccagatc tgattgaacg gaaacaccag ttg acg aat agt aaa 115
Leu Thr Asn Ser Lys
1 5
aat cct cct gcc aaa aag agc agc ggc aaa agg cgc tta aag agc gac 163
Asn Pro Pro Ala Lys Lys Ser Ser Gly Lys Arg Arg Leu Lys Ser Asp
10 15 20
tct aag ggc tac gct gta aga aac acc atc att ggt gcc att gtt gct 211
Ser Lys Gly Tyr Ala Val Arg Asn Thr Ile Ile Gly Ala Ile Val Ala
25 30 35
gtc att ttg att cca gta atg gtc ttc atg ggt gct tac atc atg gtt 259
Val Ile Leu Ile Pro Val Met Val Phe Met Gly Ala Tyr Ile Met Val
40 45 50
gat gtt cca gaa ccg gaa gag ttg gtt tca ccc cag gtt tcg cag att 307
Asp Val Pro Glu Pro Glu Glu Leu Val Ser Pro Gln Val Ser Gln Ile
55 60 65
tac gca tct gac ggt gag act gaa ttg gca cgc atc gtt cct cca gaa 355
Tyr Ala Ser Asp Gly Glu Thr Glu Leu Ala Arg Ile Val Pro Pro Glu
70 75 80 85
ggc aac cgc cag atg gtg acg atc gat cag gtg cct gac act gtg aaa 403
Gly Asn Arg Gln Met Val Thr Ile Asp Gln Val Pro Asp Thr Val Lys
90 95 100
aat gcg gtg gtg gct gcg gaa gac cga gag ttt tac aca aac ccc ggt 451
Asn Ala Val Val Ala Ala Glu Asp Arg Glu Phe Tyr Thr Asn Pro Gly
105 110 115
ttt tcc att act ggc tat gcc cga gca gca ctt ggc gta atc act ggt 499
Phe Ser Ile Thr Gly Tyr Ala Arg Ala Ala Leu Gly Val Ile Thr Gly
120 125 130
gat tct tca gcg ggt ggt ggt tcc acc att act cag cag tat gtg aag 547
Asp Ser Ser Ala Gly Gly Gly Ser Thr Ile Thr Gln Gln Tyr Val Lys
135 140 145
aag gct gtg gtt ggt gat gag cgt tcg ctg atc cgt aag gct aag gaa 595
Lys Ala Val Val Gly Asp Glu Arg Ser Leu Ile Arg Lys Ala Lys Glu
150 155 160 165
ttg gtc tat tcc gcg aag atg gcc aat gag tgg tct aag gac gag gtc 643
Leu Val Tyr Ser Ala Lys Met Ala Asn Glu Trp Ser Lys Asp Glu Val
170 175 180
ctt gag gct tat ctc aac act gtg tac ttc ggt cga aat gcc tat ggt 691
Leu Glu Ala Tyr Leu Asn Thr Val Tyr Phe Gly Arg Asn Ala Tyr Gly
185 190 195
gtg cag gct gca gct cat gca ttc ttt gat aag cca gta gaa gag ctc 739
Val Gln Ala Ala Ala His Ala Phe Phe Asp Lys Pro Val Glu Glu Leu
200 205 210
acg gct gct gag ggc gca gtg ctg gcg gcc agt att cag ctg cca agc 787
Thr Ala Ala Glu Gly Ala Val Leu Ala Ala Ser Ile Gln Leu Pro Ser
215 220 225
cag ttg gat cct tgg aca aat cca gtt gag gcg gaa acg cgt tgg aac 835
Gln Leu Asp Pro Trp Thr Asn Pro Val Glu Ala Glu Thr Arg Trp Asn
230 235 240 245
tat gtc atg gac ggc ctg gtg gaa att ggc gct atc tcg gca gag gag 883
Tyr Val Met Asp Gly Leu Val Glu Ile Gly Ala Ile Ser Ala Glu Glu
250 255 260
cgc gca gtt gct acc tac cct gaa acc act gac cct gcg tcc aac agt 931
Arg Ala Val Ala Thr Tyr Pro Glu Thr Thr Asp Pro Ala Ser Asn Ser
265 270 275
gcg tac acc gaa gcc acc ggc act aat ggt ttg att aag aac caa gtg 979
Ala Tyr Thr Glu Ala Thr Gly Thr Asn Gly Leu Ile Lys Asn Gln Val
280 285 290
atg gcg gag ttg tct gag ctt ggt atc act gag gat gat gtg caa acc 1027
Met Ala Glu Leu Ser Glu Leu Gly Ile Thr Glu Asp Asp Val Gln Thr
295 300 305
cgt ggt ttg cag gtc acc acc acc att gat cca aag act cag gaa ggt 1075
Arg Gly Leu Gln Val Thr Thr Thr Ile Asp Pro Lys Thr Gln Glu Gly
310 315 320 325
gcc gtt gaa gcg gta caa aac cag ttg gat ctt ctg tct gag aac aac 1123
Ala Val Glu Ala Val Gln Asn Gln Leu Asp Leu Leu Ser Glu Asn Asn
330 335 340
cgt gca gcg gta gtc tcc att gat cct tct aat ggt gcg gtt cgt gct 1171
Arg Ala Ala Val Val Ser Ile Asp Pro Ser Asn Gly Ala Val Arg Ala
345 350 355
tat tac ggc ggc gag gat gcg act ggt tgg gac ttt gca aac gct ccg 1219
Tyr Tyr Gly Gly Glu Asp Ala Thr Gly Trp Asp Phe Ala Asn Ala Pro
360 365 370
ctt cag acc ggt tct aca ttc aag atc ttt ggt ctg gca gca gca ctt 1267
Leu Gln Thr Gly Ser Thr Phe Lys Ile Phe Gly Leu Ala Ala Ala Leu
375 380 385
cag caa ggt att cca ctg tct cag cca tac agc tct gcg ccg gtg act 1315
Gln Gln Gly Ile Pro Leu Ser Gln Pro Tyr Ser Ser Ala Pro Val Thr
390 395 400 405
gtg ggt gat gct caa atc gga aac gtc ggt ggc agc ggt tgt ggt tcc 1363
Val Gly Asp Ala Gln Ile Gly Asn Val Gly Gly Ser Gly Cys Gly Ser
410 415 420
tgt tcc atc gag cag gcg ttg ttg cat tct tac aac acc agc ttc att 1411
Cys Ser Ile Glu Gln Ala Leu Leu His Ser Tyr Asn Thr Ser Phe Ile
425 430 435
cgt ttg cag cag gat ctg gaa aat ggt tca cag gat act gcg gac atg 1459
Arg Leu Gln Gln Asp Leu Glu Asn Gly Ser Gln Asp Thr Ala Asp Met
440 445 450
gcg cat gct ttg ggt atc gcg aag tct ttg cca act atc cct gag aca 1507
Ala His Ala Leu Gly Ile Ala Lys Ser Leu Pro Thr Ile Pro Glu Thr
455 460 465
ctg act gaa aac gga gag acc cct tat gag ggc atc atc ttg ggt cag 1555
Leu Thr Glu Asn Gly Glu Thr Pro Tyr Glu Gly Ile Ile Leu Gly Gln
470 475 480 485
tat gag tcc cgc cca ctt gat atg gct tct gcg atg gca act atc gct 1603
Tyr Glu Ser Arg Pro Leu Asp Met Ala Ser Ala Met Ala Thr Ile Ala
490 495 500
aat gaa ggt gtc tgg cac cgc ccg cac ttc gtg tcc aag gtg gag act 1651
Asn Glu Gly Val Trp His Arg Pro His Phe Val Ser Lys Val Glu Thr
505 510 515
gtc agc ggt gag gtt ctc tac gag ttc gag gat ggc gac ggc gag cgt 1699
Val Ser Gly Glu Val Leu Tyr Glu Phe Glu Asp Gly Asp Gly Glu Arg
520 525 530
cgt gtt tct gaa aag gtt gca ctg aat ctg ctc aag gcc atg ggg cca 1747
Arg Val Ser Glu Lys Val Ala Leu Asn Leu Leu Lys Ala Met Gly Pro
535 540 545
atc gct gca tac tcc aac gga aac gct ctg gct gat ggc cag gtt tct 1795
Ile Ala Ala Tyr Ser Asn Gly Asn Ala Leu Ala Asp Gly Gln Val Ser
550 555 560 565
gca tcc aag act ggt acc act cag ctt ggt gat acc ggt gca aac aag 1843
Ala Ser Lys Thr Gly Thr Thr Gln Leu Gly Asp Thr Gly Ala Asn Lys
570 575 580
gat gcg tgg atg ttg ggt gcg gca cct cag cta gct act gcg gtg tgg 1891
Asp Ala Trp Met Leu Gly Ala Ala Pro Gln Leu Ala Thr Ala Val Trp
585 590 595
gtc gga act gct gat aac act gca ttg tat aac acc tgg ggt ggc agt 1939
Val Gly Thr Ala Asp Asn Thr Ala Leu Tyr Asn Thr Trp Gly Gly Ser
600 605 610
atg tat ggt tct aac tcc cct gcc acg atc tgg aag cag acc atg gat 1987
Met Tyr Gly Ser Asn Ser Pro Ala Thr Ile Trp Lys Gln Thr Met Asp
615 620 625
aac gcc ctc gag aac tcc cct ctc gaa act tgg gat atc gct cca gca 2035
Asn Ala Leu Glu Asn Ser Pro Leu Glu Thr Trp Asp Ile Ala Pro Ala
630 635 640 645
ttg ggg tac ggt aac cca cca gtt ccg gaa tat gtg tgg act cca agt 2083
Leu Gly Tyr Gly Asn Pro Pro Val Pro Glu Tyr Val Trp Thr Pro Ser
650 655 660
cca aac atc gcg act aat gat cca gaa gga gca acc gag gaa gct cca 2131
Pro Asn Ile Ala Thr Asn Asp Pro Glu Gly Ala Thr Glu Glu Ala Pro
665 670 675
gtg gag gat cca aat gca gta atc gat acc cct gct gta gat ccc act 2179
Val Glu Asp Pro Asn Ala Val Ile Asp Thr Pro Ala Val Asp Pro Thr
680 685 690
gca cct gca gag gag acc ggt aac ggt cag gta gaa atc ctg ccg ggg 2227
Ala Pro Ala Glu Glu Thr Gly Asn Gly Gln Val Glu Ile Leu Pro Gly
695 700 705
ctg act atc ccg gga gat ctc tta ggg atc ggc taaaatccgg tcgtagccta 2280
Leu Thr Ile Pro Gly Asp Leu Leu Gly Ile Gly
710 715 720
aacgacctta 2290
<210>66
<211>720
<212>PRT
<213>谷氨酸棒状杆菌
<400>66
Leu Thr Asn Ser Lys Asn Pro Pro Ala Lys Lys Ser Ser Gly Lys Arg
1 5 10 15
Arg Leu Lys Ser Asp Ser Lys Gly Tyr Ala Val Arg Asn Thr Ile Ile
20 25 30
Gly Ala Ile Val Ala Val Ile Leu Ile Pro Val Met Val Phe Met Gly
35 40 45
Ala Tyr Ile Met Val Asp Val Pro Glu Pro Glu Glu Leu Val Ser Pro
50 55 60
Gln Val Ser Gln Ile Tyr Ala Ser Asp Gly Glu Thr Glu Leu Ala Arg
65 70 75 80
Ile Val Pro Pro Glu Gly Asn Arg Gln Met Val Thr Ile Asp Gln Val
85 90 95
Pro Asp Thr Val Lys Asn Ala Val Val Ala Ala Glu Asp Arg Glu Phe
100 105 110
Tyr Thr Asn Pro Gly Phe Ser Ile Thr Gly Tyr Ala Arg Ala Ala Leu
115 120 125
Gly Val Ile Thr Gly Asp Ser Ser Ala Gly Gly Gly Ser Thr Ile Thr
130 135 140
Gln Gln Tyr Val Lys Lys Ala Val Val Gly Asp Glu Arg Ser Leu Ile
145 150 155 160
Arg Lys Ala Lys Glu Leu Val Tyr Ser Ala Lys Met Ala Ash Glu Trp
165 170 175
Ser Lys Asp Glu Val Leu Glu Ala Tyr Leu Asn Thr Val Tyr Phe Gly
180 185 190
Arg Asn Ala Tyr Gly Val Gln Ala Ala Ala His Ala Phe Phe Asp Lys
195 200 205
Pro Val Glu Glu Leu Thr Ala Ala Glu Gly Ala Val Leu Ala Ala Ser
210 215 220
Ile Gln Leu Pro Ser Gln Leu Asp Pro Trp Thr Asn Pro Val Glu Ala
225 230 235 240
Glu Thr Arg Trp Asn Tyr Val Met Asp Gly Leu Val Glu Ile Gly Ala
245 250 255
Ile Ser Ala Glu Glu Arg Ala Val Ala Thr Tyr Pro Glu Thr Thr Asp
260 265 270
Pro Ala Ser Asn Ser Ala Tyr Thr Glu Ala Thr Gly Thr Asn Gly Leu
275 280 285
Ile Lys Asn Gln Val Met Ala Glu Leu Ser Glu Leu Gly Ile Thr Glu
290 295 300
Asp Asp Val Gln Thr Arg Gly Leu Gln Val Thr Thr Thr Ile Asp Pro
305 310 315 320
Lys Thr Gln Glu Gly Ala Val Glu Ala Val Gln Asn Gln Leu Asp Leu
325 330 335
Leu Ser Glu Asn Asn Arg Ala Ala Val Val Ser Ile Asp Pro Ser Asn
340 345 350
Gly Ala Val Arg Ala Tyr Tyr Gly Gly Glu Asp Ala Thr Gly Trp Asp
355 360 365
Phe Ala Asn Ala Pro Leu Gln Thr Gly Ser Thr Phe Lys Ile Phe Gly
370 375 380
Leu Ala Ala Ala Leu Gln Gln Gly Ile Pro Leu Ser Gln Pro Tyr Ser
385 390 395 400
Ser Ala Pro Val Thr Val Gly Asp Ala Gln Ile Gly Asn Val Gly Gly
405 410 415
Ser Gly Cys Gly Ser Cys Ser Ile Glu Gln Ala Leu Leu His Ser Tyr
420 425 430
Asn Thr Ser Phe Ile Arg Leu Gln Gln Asp Leu Glu Asn Gly Ser Gln
435 440 445
Asp Thr Ala Asp Met Ala His Ala Leu Gly Ile Ala Lys Ser Leu Pro
450 455 460
Thr Ile Pro Glu Thr Leu Thr Glu Asn Gly Glu Thr Pro Tyr Glu Gly
465 470 475 480
Ile Ile Leu Gly Gln Tyr Glu Ser Arg Pro Leu Asp Met Ala Ser Ala
485 490 495
Met Ala Thr Ile Ala Asn Glu Gly Val Trp His Arg Pro His Phe Val
500 505 510
Ser Lys Val Glu Thr Val Ser Gly Glu Val Leu Tyr Glu Phe Glu Asp
515 520 525
Gly Asp Gly Glu Arg Arg Val Ser Glu Lys Val Ala Leu Asn Leu Leu
530 535 540
Lys Ala Met Gly Pro Ile Ala Ala Tyr Ser Asn Gly Asn Ala Leu Ala
545 550 555 560
Asp Gly Gln Val Ser Ala Ser Lys Thr Gly Thr Thr Gln Leu Gly Asp
565 570 575
Thr Gly Ala Asn Lys Asp Ala Trp Met Leu Gly Ala Ala Pro Gln Leu
580 585 590
Ala Thr Ala Val Trp Val Gly Thr Ala Asp Asn Thr Ala Leu Tyr Asn
595 600 605
Thr Trp Gly Gly Ser Met Tyr Gly Ser Asn Ser Pro Ala Thr Ile Trp
610 615 620
Lys Gln Thr Met Asp Asn Ala Leu Glu Asn Ser Pro Leu Glu Thr Trp
625 630 635 640
Asp Ile Ala Pro Ala Leu Gly Tyr Gly Asn Pro Pro Val Pro Glu Tyr
645 650 655
Val Trp Thr Pro Ser Pro Asn Ile Ala Thr Asn Asp Pro Glu Gly Ala
660 665 670
Thr Glu Glu Ala Pro Val Glu Asp Pro Asn Ala Val Ile Asp Thr Pro
675 680 685
Ala Val Asp Pro Thr Ala Pro Ala Glu Glu Thr Gly Asn Gly Gln Val
690 695 700
Glu Ile Leu Pro Gly Leu Thr Ile Pro Gly Asp Leu Leu Gly Ile Gly
705 710 715 720
<210>67
<211>1066
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1036)
<223>RXA00552
<400>67
ccgccaacaa ggcagcaaag ctcgatccaa ttgacgcctt gcgttatgagtaaaagcctc 60
gtttttaagg tagccacaca tcgcactaga ctgaagaact gtg gct acc tca aaa 115
Val Ala Thr Ser Lys
1 5
att ctt ctt tat tac gca ttc acc ccg ctc tct gac cct aaa gcg gtt 163
Ile Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser Asp Pro Lys Ala Val
10 15 20
cag ctg tgg cag cgt gag ctc tgc gag tca ctg aat ctt cgt ggc cgc 211
Gln Leu Trp Gln Arg Glu Leu Cys Glu Ser Leu Asn Leu Arg Gly Arg
25 30 35
atc ctg atc tcc act cac ggc atc aat gga acc gtg ggc gga gat att 259
Ile Leu Ile Ser Thr His Gly Ile Asn Gly Thr Val Gly Gly Asp Ile
40 45 50
gat gat tgc aag gcg tac att aaa aag acc cgc gag tac cca ggt ttc 307
Asp Asp Cys Lys Ala Tyr Ile Lys Lys Thr Arg Glu Tyr Pro Gly Phe
55 60 65
aac cgc atg cag ttt aag tgg tcc gag ggt ggc gct gag gat ttc cca 355
Asn Arg Met Gln Phe Lys Trp Ser Glu Gly Gly Ala Glu Asp Phe Pro
70 75 80 85
aag ctc agt gtc aaa gtc cgc gat gag atc gtt gcc ttc ggc gct cca 403
Lys Leu Ser Val Lys Val Arg Asp Glu Ile Val Ala Phe Gly Ala Pro
90 95 100
gat gag ctc aaa gtg gat gaa aac ggc gtc gtc ggt ggc ggc gtt cac 451
Asp Glu Leu Lys Val Asp Glu Asn Gly Val Val Gly Gly Gly Val His
105 110 115
ctg aaa cca cag cag gtc aat gag ctt gtg gaa gcc cgt ggc gat gaa 499
Leu Lys Pro Gln Gln Val Asn Glu Leu Val Glu Ala Arg Gly Asp Glu
120 125 130
gtt gtg ttc ttt gac ggc cgc aac gca atg gaa gcc cag atc ggc aag 547
Val Val Phe Phe Asp Gly Arg Asn Ala Met Glu Ala Gln Ile Gly Lys
135 140 145
ttc aag gac gct gtt gtc cct gac gta gaa acc act cat gat ttc atc 595
Phe Lys Asp Ala Val Val Pro Asp Val Glu Thr Thr His Asp Phe Ile
150 155 160 165
gca gaa att gag tct gga aaa tac gac gat ctc aaa gac aag cct gtg 643
Ala Glu Ile Glu Ser Gly Lys Tyr Asp Asp Leu Lys Asp Lys Pro Val
170 175 180
gtc acc tac tgc acc ggc gga att cgt tgt gag atc ctg agt tca ctc 691
Val Thr Tyr Cys Thr Gly Gly Ile Arg Cys Glu Ile Leu Ser Set Leu
185 190 195
atg atc aac cgt ggt ttc aaa gag gtc tac caa atc gat ggc ggc atc 739
Met Ile Asn Arg Gly Phe Lys Glu Val Tyr Gln Ile Asp Gly Gly Ile
200 205 210
gtt cgc tac ggc gag cag ttt ggc aac aag ggc ctg tgg gaa ggc tcc 787
Val Arg Tyr Gly Glu Gln Phe Gly Asn Lys Gly Leu Trp Glu Gly Ser
215 220 225
ctc tac gtt ttc gat aag cgc atg cat atg gaa ttc ggc gag gat tac 835
Leu Tyr Val Phe Asp Lys Arg Met His Met Glu Phe Gly Glu Asp Tyr
230 235 240 245
aaa gag gtc gga cac tgc atc cat tgc gat act ccc acc aac aaa ttt 883
Lys Glu Val Gly His Cys Ile His Cys Asp Thr Pro Thr Asn Lys Phe
250 255 260
gag cac tgc ctc aac gaa gat gat tgc cgc gag ctc gtg ttg atg tgc 931
Glu His Cys Leu Asn Glu Asp Asp Cys Arg Glu Leu Val Leu Met Cys
265 270 275
cct gat tgc ttc gcc aat gtt gag acc cgt cat tgc aag cgc gaa cgc 979
Pro Asp Cys Phe Ala Asn Val Glu Thr Arg His Cys Lys Arg Glu Arg
280 285 290
tgt gca gca att gct gcg gat ttc gct gag caa gga att gat ccg ctc 1027
Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu Gln Gly Ile Asp Pro Leu
295 300 305
gtt act tct taaaaagggt atggtggctg ggtatgagca 1066
Val Thr Ser
310
<210>68
<211>312
<212>PRT
<213>谷氨酸棒状杆菌
<400>68
Val Ala Thr Ser Lys Ile Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser
1 5 10 15
Asp Pro Lys Ala Val Gln Leu Trp Gln Arg Glu Leu Cys Glu Ser Leu
20 25 30
Asn Leu Arg Gly Arg Ile Leu Ile Ser Thr His Gly Ile Asn Gly Thr
35 40 45
Val Gly Gly Asp Ile Asp Asp Cys Lys Ala Tyr Ile Lys Lys Thr Arg
50 55 60
Glu Tyr Pro Gly Phe Asn Arg Met Gln Phe Lys Trp Ser G1u Gly G1y
65 70 75 80
Ala Glu Asp Phe Pro Lys Leu Ser Val Lys Val Arg Asp Glu Ile Val
85 90 95
Ala Phe Gly Ala Pro Asp Glu Leu Lys Val Asp Glu Asn Gly Val Val
100 105 110
Gly Gly Gly Val His Leu Lys Pro Gln Gln Val Asn Glu Leu Val Glu
115 120 125
Ala Arg Gly Asp Glu Val Val Phe Phe Asp Gly Arg Asn Ala Met Glu
130 135 140
Ala Gln Ile Gly Lys Phe Lys Asp Ala Val Val Pro Asp Val Glu Thr
145 150 155 160
Thr His Asp Phe Ile Ala Glu Ile Glu Ser Gly Lys Tyr Asp Asp Leu
165 170 175
Lys Asp Lys Pro Val Val Thr Tyr Cys Thr Gly Gly Ile Arg Cys Glu
180 185 190
Ile Leu Ser Ser Leu Met Ile Asn Arg Gly Phe Lys Glu Val Tyr Gln
195 200 205
Ile Asp Gly Gly Ile Val Arg Tyr Gly Glu Gln Phe Gly Asn Lys Gly
210 215 220
Leu Trp Glu Gly Ser Leu Tyr Val Phe Asp Lys Arg Met His Met Glu
225 230 235 240
Phe Gly Glu Asp Tyr Lys Glu Val Gly His Cys Ile His Cys Asp Thr
245 250 255
Pro Thr Asn Lys Phe Glu His Cys Leu Asr Glu Asp Asp Cys Arg Glu
260 265 270
Leu Val Leu Met Cys Pro Asp Cys Phe Ala Asn Val Glu Thr Arg His
275 280 285
Cys Lys Arg Glu Arg Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu Gln
290 295 300
Gly Ile Asp Pro Leu Val Thr Ser
305 310
<210>69
<211>952
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<22l>CDS
<222>(101)..(922)
<223>RXA00576
<400>69
tgatgcccaa tttttgggcg accactggac gagggtgtgg gccctcgcgg cgtagggttt 60
ggagccactc cggtgggttt tcctgcaggt tcttgaactc ttg gtg agt caa tgc 115
Leu Val Ser Gln Cys
1 5
acc tgt ttg gaa ctc ctc tgg cgt ggc ggg caa aaa cag tcc gag ctt 163
Thr Cys Leu Glu Leu Leu Trp Arg Gly Gly Gln Lys Gln Ser Glu Leu
10 15 20
ctt agc ggc ggt ctg tgg ctt cat agc cct gcc cga tgg ctg gcg tac 211
Leu Ser Gly Gly Leu Trp Leu His Ser Pro Ala Arg Trp Leu Ala Tyr
25 30 35
aga ttc ttc gtt cac gcc cac aac ggt agc att gtt ttc atg ctg acc 259
Arg Phe Phe Val His Ala His Asn Gly Ser Ile Val Phe Met Leu Thr
40 45 50
tta agt ttc atc act ggc acg gag cca gga aag tgg ttt acc cga ttc 307
Leu Ser Phe Ile Thr Gly Thr Glu Pro Gly Lys Trp Phe Thr Arg Phe
55 60 65
cga gat cgg act cat cac ggt gga ctt gag acc ctt gat tcg gac gac 355
Arg Asp Arg Thr His His Gly Gly Leu Glu Thr Leu Asp Ser Asp Asp
70 75 80 85
gcc cta ggc ctc atg ctc gct ggc cag gca caa ctc gcc tta acc agg 403
Ala Leu Gly Leu Met Leu Ala Gly Gln Ala Gln Leu Ala Leu Thr Arg
90 95 100
ctt cca gat gcg cgt atc gac gcc tcc ctc cac gtg gtc act ctt tat 451
Leu Pro Asp Ala Arg Ile Asp Ala Ser Leu His Val Val Thr Leu Tyr
105 110 115
cag gaa caa ccc gga gtc gct ttt cca aaa gac ttt ttc ctc agt gcc 499
Gln Glu Gln Pro Gly Val Ala Phe Pro Lys Asp Phe Phe Leu Ser Ala
120 125 130
gaa gaa ggc gcg gtg gac ctt gcg gac tta gat ggg gag atc atc aac 547
Glu Glu Gly Ala Val Asp Leu Ala Asp Leu Asp Gly Glu Ile Ile Asn
135 140 145
tgg tcc atg ccc gat agt ggg gaa gtc gat gcc gct gct gtt cgc gat 595
Trp Ser Met Pro Asp Ser Gly Glu Val Asp Ala Ala Ala Val Arg Asp
150 155 160 165
gct ctg caa atc gtg gca gcg aac gtc ggt gtg gtg atc gcc ccc agg 643
Ala Leu Gln Ile Val Ala Ala Asn Val Gly Val Val Ile Ala Pro Arg
170 175 180
cca ctt ctc aag gtg ttg agc aag aaa ttg gtg gag cac cgg gac atc 691
Pro Leu Leu Lys Val Leu Ser Lys Lys Leu Val Glu His Arg Asp Ile
185 190 195
aag ggt gga act gaa aca tct atc gcc ctg gta tgg aag aaa gat gag 739
Lys Gly Gly Thr Glu Thr Ser Ile Ala Leu Val Trp Lys Lys Asp Glu
200 205 210
gat tct gaa gag att caa gac ttc gtc ggt att gct cgc ggt cga acg 787
Asp Ser Glu Glu Ile Gln Asp Phe Val Gly Ile Ala Arg Gly Arg Thr
215 220 225
cgg aat tcc agc agg cag caa acg gta aag ctc agt gct cgt gaa aaa 835
Arg Asn Ser Ser Arg Gln Gln Thr Val Lys Leu Ser Ala Arg Glu Lys
230 235 240 245
aca ctg gca aaa caa gcc cgc agg cag ggg gag aaa cca aaa act ccg 883
Thr Leu Ala Lys Gln Ala Arg Arg Gln Gly Glu Lys Pro Lys Thr Pro
250 255 260
aag cgt ccg cag gca aga aaa cgt ccc gga aaa cgg cga taggggtcac 932
Lys Arg Pro Gln Ala Arg Lys Arg Pro Gly Lys Arg Arg
265 270
ccgcgcatgt ccggttgccg 952
<210>70
<211>274
<212>PRT
<213>谷氨酸棒状杆菌
<400>70
Leu Val Ser Gln Cys Thr Cys Leu Glu Leu Leu Trp Arg Gly Gly Gln
1 5 10 15
Lys Gln Ser Glu Leu Leu Ser Gly Gly Leu Trp Leu His Ser Pro Ala
20 25 30
Arg Trp Leu Ala Tyr Arg Phe Phe Val His Ala His Asn Gly Ser Ile
35 40 45
Val Phe Met Leu Thr Leu Ser Phe Ile Thr Gly Thr Glu Pro Gly Lys
50 55 60
Trp Phe Thr Arg Phe Arg Asp Arg Thr His His Gly Gly Leu Glu Thr
65 70 75 80
Leu Asp Ser Asp Asp Ala Leu Gly Leu Met Leu Ala Gly Gln Ala Gln
85 90 95
Leu Ala Leu Thr Arg Leu Pro Asp Ala Arg Ile Asp Ala Ser Leu His
100 105 110
Val Val Thr Leu Tyr Gln Glu Gln Pro Gly Val Ala Phe Pro Lys Asp
115 120 125
Phe Phe Leu Ser Ala Glu Glu Gly Ala Val Asp Leu Ala Asp Leu Asp
130 135 140
Gly Glu Ile Ile Asn Trp Ser Met Pro Asp Ser Gly Glu Val Asp Ala
145 150 155 160
Ala Ala Val Arg Asp Ala Leu Gln Ile Val Ala Ala Asn Val Gly Val
165 170 175
Val Ile Ala Pro Arg Pro Leu Leu Lys Val Leu Ser Lys Lys Leu Val
180 185 190
Glu His Arg Asp Ile Lys Gly Gly Thr Glu Thr Ser Ile Ala Leu Val
195 200 205
Trp Lys Lys Asp Glu Asp Ser Glu Glu Ile Gln Asp Phe Val Gly Ile
210 215 220
Ala Arg Gly Arg Thr Arg Asn Ser Ser Arg Gln Gln Thr Val Lys Leu
225 230 235 240
Ser Ala Arg Glu Lys Thr Leu Ala Lys Gln Ala Arg Arg Gln Gly Glu
245 250 255
Lys Pro Lys Thr Pro Lys Arg Pro Gln Ala Arg Lys Arg Pro Gly Lys
260 265 270
Arg Arg
<210>71
<211>1606
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1576)
<223>RXA00611
<400>71
ctttaaattg actatttaca aaactacttg atgagttagc aagagcacat ttcggtgcgc 60
ctccctgcta aacctcgaaa caataactag attgcacact atg tct gcc gct gct 115
Met Ser Ala Ala Ala
1 5
tct cga tca aga aac atc ttg act gcc ctc ctg cta ttg gtc ccg ctc 163
Ser Arg Ser Arg Asn Ile Leu Thr Ala Leu Leu Leu Leu Val Pro Leu
10 15 20
atc gca ggt acc atc tac gcc tcc gcc atg ggc ttg gac gtc tcc cgc 211
Ile Ala Gly Thr Ile Tyr Ala Ser Ala Met Gly Leu Asp Val Ser Arg
25 30 35
gca tgg tcc tca gca gat gaa gta acg ggg gca cct gca gcc tcc gta 259
Ala Trp Ser Ser Ala Asp Glu Val Thr Gly Ala Pro Ala Ala Ser Val
40 45 50
gcc acc aac aac cag gag ctt atc gaa gcc cgt cgc gcc gcc gga gaa 307
Ala Thr Asn Asn Gln Glu Leu Ile Glu Ala Arg Arg Ala Ala Gly Glu
55 60 65
gcc ggc gcc caa gcg ggg ttc ctc acc tca gga act gaa gag cta acc 355
Ala Gly Ala Gln Ala Gly Phe Leu Thr Ser Gly Thr Glu Glu Leu Thr
70 75 80 85
agc gga aca caa gag ctt atc gac ggc gca gcc cca ctc gaa gaa ggc 403
Ser Gly Thr Gln Glu Leu Ile Asp Gly Ala Ala Pro Leu Glu Glu Gly
90 95 100
gtc tca gcc gcg gct gac ggt gca gcg caa ctc cac gat ggc ctc atc 451
Val Ser Ala Ala Ala Asp Gly Ala Ala Gln Leu His Asp Gly Leu Ile
105 110 115
caa ctc cag gcc ggc act gga caa atg gga acc ggc gcc acc gaa att 499
Gln Leu Gln Ala Gly Thr Gly Gln Met Gly Thr Gly Ala Thr Glu Ile
120 125 130
gcc gac ggc gtc caa aac gca gtc gaa caa ctt ggc ggt ctt gtc gtt 547
Ala Asp Gly Val Gln Asn Ala Val Glu Gln Leu Gly Gly Leu Val Val
135 140 145
gta cag cag caa ctc tta ggc gcc ctc aac gaa gca gac aaa caa ctc 595
Val Gln Gln Gln Leu Leu Gly Ala Leu Asn Glu Ala Asp Lys Gln Leu
150 155 160 165
gcc tcg agc aaa atc ccc gaa gcc gaa gac ctg cgc aaa caa atc acc 643
Ala Ser Ser Lys Ile Pro Glu Ala Glu Asp Leu Arg Lys Gln Ile Thr
170 175 180
gaa gtc cga gga cac ctc gaa aac ttc ggc atc tcc gta gaa atg acc 691
Glu Val Arg Gly His Leu Glu Asn Phe Gly Ile Ser Val Glu Met Thr
185 190 195
gac caa ctc gat caa cta cgt tcc ggc acc cgc gac ctg gct aac caa 739
Asp Gln Leu Asp Gln Leu Arg Ser Gly Thr Arg Asp Leu Ala Asn Gln
200 205 210
ctc gca gtc ccc ggc tac gga ttc cac gat ggt att tac agc gcc acc 787
Leu Ala Val Pro Gly Tyr Gly Phe His Asp Gly Ile Tyr Ser Ala Thr
215 220 225
aat gga gca gcc gaa tta tct gca ggt ttg caa gag cta gaa gca ggc 835
Asn Gly Ala Ala Glu Leu Ser Ala Gly Leu Gln Glu Leu Glu Ala Gly
230 235 240 245
gtt ggg act gcc gtc gaa ggc ttc acc gca ctc gat gaa ggt gca aac 883
Val Gly Thr Ala Val Glu Gly Phe Thr Ala Leu Asp Glu Gly Ala Asn
250 255 260
cga tta gac tcc atg gcc acc ctc aac gaa gaa aaa acc tcc gca gtc 931
Arg Leu Asp Ser Met Ala Thr Leu Asn Glu Glu Lys Thr Ser Ala Val
265 270 275
caa cga gcc ctc ccg gta ccc caa gta ccc gcc ggc aca atc gaa ggc 979
Gln Arg Ala Leu Pro Val Pro Gln Val Pro Ala Gly Thr Ile Glu Gly
280 285 290
acc gca gac gaa gaa cgc acc agc gca cta gcg ccc atg tac gcc ttc 1027
Thr Ala Asp Glu Glu Arg Thr Ser Ala Leu Ala Pro Met Tyr Ala Phe
295 300 305
tta att tca gca ttg gtc atg ctg gcc ggt gca gca ctt gga tgg gca 1075
Leu Ile Ser Ala Leu Val Met Leu Ala Gly Ala Ala Leu Gly Trp Ala
310 315 320 325
aca cta aaa aac aag tgg ctg atg gcc ttt gct gtc att ggt gtc act 1123
Thr Leu Lys Asn Lys Trp Leu Met Ala Phe Ala Val Ile Gly Val Thr
330 335 340
gca atc ggt ggc att atc ttg ttc acc gta gct ttg ggc ata tcc att 1171
Ala Ile Gly Gly Ile Ile Leu Phe Thr Val Ala Leu Gly Ile Ser Ile
345 350 355
gga gct ttg ttt gga gcc tta gga att ttg ctt ctt gcc act gtt gtt 1219
Gly Ala Leu Phe Gly Ala Leu Gly Ile Leu Leu Leu Ala Thr Val Val
360 365 370
gcc ggt att ctc tcc cgg att ttg ctt gac gtg ttg gga act acc ggg 1267
Ala Gly Ile Leu Ser Arg Ile Leu Leu Asp Val Leu Gly Thr Thr Gly
375 380 385
gct att gtg gtt tct gtt ctg gga tgg gta gcc caa gct gca gta att 1315
Ala Ile Val Val Ser Val Leu Gly Trp Val Ala Gln Ala Ala Val Ile
390 395 400 405
ggc cat gta tgg agt gtt acc gct gta tcc gat atc gca ctt gtt tgg 1363
Gly His Val Trp Ser Val Thr Ala Val Ser Asp Ile Ala Leu Val Trp
410 415 420
cga gtc gtc gca ggc atg atg cca ctg cat tat cca acc ttt gca gtg 1411
Arg Val Val Ala Gly Met Met Pro Leu His Tyr Pro Thr Phe Ala Val
425 430 435
acc tcc att ggt aat ggc gga tca gct gca gct atc tgg atg tct gtt 1459
Thr Ser Ile Gly Asn Gly Gly Ser Ala Ala Ala Ile Trp Met Ser Val
440 445 450
gct gtc ttg ttg gca atg gga gcg atc gga gct gtt gcg ctt cgg aag 1507
Ala Val Leu Leu Ala Met Gly Ala Ile Gly Ala Val Ala Leu Arg Lys
455 460 465
cca aag gcg gtt gcg gtt gag gtt gaa gaa gct gtt gat gct gat gca 1555
Pro Lys Ala Val Ala Val Glu Val Glu Glu Ala Val Asp Ala Asp Ala
470 475 480 485
gac caa gca gcc tcg gaa tcc tgatggtttt ggtctgattc gacctgttct 1606
Asp Gln Ala Ala Ser Glu Ser
490
<210>72
<211>492
<212>PRT
<213>谷氨酸棒状杆菌
<400>72
Met Ser Ala Ala Ala Ser Arg Ser Arg Asn Ile Leu Thr Ala Leu Leu
1 5 10 15
Leu Leu Val Pro Leu Ile Ala Gly Thr Ile Tyr Ala Ser Ala Met Gly
20 25 30
Leu Asp Val Ser Arg Ala Trp Ser Ser Ala Asp Glu Val Thr Gly Ala
35 40 45
Pro Ala Ala Ser Val Ala Thr Asn Asn Gln Glu Leu Ile Glu Ala Arg
50 55 60
Arg Ala Ala Gly Glu Ala Gly Ala Gln Ala Gly Phe Leu Thr Ser Gly
65 70 75 80
Thr Glu Glu Leu Thr Ser Gly Thr Gln Glu Leu Ile Asp Gly Ala Ala
85 90 95
Pro Leu Glu Glu Gly Val Ser Ala Ala Ala Asp Gly Ala Ala Gln Leu
100 105 110
His Asp Gly Leu Ile Gln Leu Gln Ala Gly Thr Gly Gln Met Gly Thr
115 120 125
Gly Ala Thr Glu Ile Ala Asp Gly Val Gln Asn Ala Val Glu Gln Leu
130 135 140
Gly Gly Leu Val Val Val Gln Gln Gln Leu Leu Gly Ala Leu Asn Glu
145 150 155 160
Ala Asp Lys Gln Leu Ala Ser Ser Lys Ile Pro Glu Ala Glu Asp Leu
165 170 175
Arg Lys Gln Ile Thr Glu Val Arg Gly His Leu Glu Asn Phe Gly Ile
180 185 190
Ser Val Glu Met Thr Asp Gln Leu Asp Gln Leu Arg Ser Gly Thr Arg
195 200 205
Asp Leu Ala Asn Gln Leu Ala Val Pro Gly Tyr Gly Phe His Asp Gly
210 215 220
Ile Tyr Ser Ala Thr Asn Gly Ala Ala Glu Leu Ser Ala Gly Leu Gln
225 230 235 240
Glu Leu Glu Ala Gly Val Gly Thr Ala Val Glu Gly Phe Thr Ala Leu
245 250 255
Asp Glu Gly Ala Asn Arg Leu Asp Ser Met Ala Thr Leu Asn Glu Glu
260 265 270
Lys Thr Ser Ala Val Gln Arg Ala Leu Pro Val Pro Gln Val Pro Ala
275 280 285
Gly Thr Ile Glu Gly Thr Ala Asp Glu Glu Arg Thr Ser Ala Leu Ala
290 295 300
Pro Met Tyr Ala Phe Leu Ile Ser Ala Leu Val Met Leu Ala Gly Ala
305 310 315 320
Ala Leu Gly Trp Ala Thr Leu Lys Asn Lys Trp Leu Met Ala Phe Ala
325 330 335
Val Ile Gly Val Thr Ala Ile Gly Gly Ile Ile Leu Phe Thr Val Ala
340 345 350
Leu Gly Ile Ser Ile Gly Ala Leu Phe Gly Ala Leu Gly Ile Leu Leu
355 360 365
Leu Ala Thr Val Val Ala Gly Ile Leu Ser Arg Ile Leu Leu Asp Val
370 375 380
Leu Gly Thr Thr Gly Ala Ile Val Val Ser Val Leu Gly Trp Val Ala
385 390 395 400
Gln Ala Ala Val Ile Gly His Val Trp Ser Val Thr Ala Val Ser Asp
405 410 415
Ile Ala Leu Val Trp Arg Val Val Ala Gly Met Met Pro Leu His Tyr
420 425 430
Pro Thr Phe Ala Val Thr Ser Ile Gly Asn Gly Gly Ser Ala Ala Ala
435 440 445
Ile Trp Met Ser Val Ala Val Leu Leu Ala Met Gly Ala Ile Gly Ala
450 455 460
Val Ala Leu Arg Lys Pro Lys Ala Val Ala Val Glu Val Glu Glu Ala
465 470 475 480
Val Asp Ala Asp Ala Asp Gln Ala Ala Ser Glu Ser
485 490
<210>73
<211>1045
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1015)
<223>RXA00614
<400>73
cagtggtgtc cttgtcgcgg gccttcatag cggtggtcag atctgcgcgg attttgtctt 60
ttagttcact catgtttgcc cagagtactc cccaggtagg gtg aat aag gtg tca 115
Val Asn Lys Val Ser
1 5
act atc gct aaa aca ctc act ttt tca agt gca gcc cta ctc gga gct 163
Thr Ile Ala Lys Thr Leu Thr Phe Ser Ser Ala Ala Leu Leu Gly Ala
10 15 20
ggt atc gcc acc gct gca tgg gga tac tca gag ctc aaa aaa ttc gag 211
Gly Ile Ala Thr Ala Ala Trp Gly Tyr Ser Glu Leu Lys Lys Phe Glu
25 30 35
ctc aaa aca gta gaa ctg cca att ttg aag cct gga acg ctc cgt gga 259
Leu Lys Thr Val Glu Leu Pro Ile Leu Lys Pro Gly Thr Leu Arg Gly
40 45 50
aag aag gaa ttc cgc ctt ctc cac atc tct gat ctc cac atg atc cca 307
Lys Lys Glu Phe Arg Leu Leu His Ile Ser Asp Leu His Met Ile Pro
55 60 65
ggc caa gaa acc aaa aaa gca tgg gtc tcc gca ctc gat tca cta agc 355
Gly Gln Glu Thr Lys Lys Ala Trp Val Ser Ala Leu Asp Ser Leu Ser
70 75 80 85
ccc gat ttg gtg atc aac acc ggt gac aac ctt agc gat gaa aaa gca 403
Pro Asp Leu Val Ile Asn Thr Gly Asp Asn Leu Ser Asp Glu Lys Ala
90 95 100
gtc ccc gac gtc ctc cgc gca ctc ggc cca ctg atg aac cgc ccc ggc 451
Val Pro Asp Val Leu Arg Ala Leu Gly Pro Leu Met Asn Arg Pro Gly
105 110 115
gcg ttc gtc ttc gga acc aac gat tac tgg gca ccc cgc ccc gtc aat 499
Ala Phe Val Phe Gly Thr Asn Asp Tyr Trp Ala Pro Arg Pro Val Asn
120 125 130
cct ttc ggc tac ctc ttc ggt aaa aaa cgc gaa gtg agc cac atc gac 547
Pro Phe Gly Tyr Leu Phe Gly Lys Lys Arg Glu Val Ser His Ile Asp
135 140 145
ctc ccc tgg cga gcc atg cga gct gct ttc atc gaa cac gga tgg caa 595
Leu Pro Trp Arg Ala Met Arg Ala Ala Phe Ile Glu His Gly Trp Gln
150 155 160 165
gac gcc aac caa aag cga ctc gaa ttc caa gta ggt tcc gtc cgc ctc 643
Asp Ala Asn Gln Lys Arg Leu Glu Phe Gln Val Gly Ser Val Arg Leu
170 175 180
gcc atc tca ggt gtt gat gac ccc cac cat gac ctc gac gac tac aca 691
Ala Ile Ser Gly Val Asp Asp Pro His His Asp Leu Asp Asp Tyr Thr
185 190 195
gag atc gca ggg gca cca aac gtg gac gct gat ctg tcc ata gcg ttg 739
Glu Ile Ala Gly Ala Pro Asn Val Asp Ala Asp Leu Ser Ile Ala Leu
200 205 210
ctt cac gca cca gaa cct cga gtc ctc gct cag ttc gaa gcc gac ggc 787
Leu His Ala Pro Glu Pro Arg Val Leu Ala Gln Phe Glu Ala Asp Gly
215 220 225
tac cag ctt tcc ctc tcc ggc cac acc cac ggc ggt cag ctt tgt ctt 835
Tyr Gln Leu Ser Leu Ser Gly His Thr His Gly Gly Gln Leu Cys Leu
230 235 240 245
ccg ggc agc aag cca att gtc acc aac tgt gga atc gac cgc aaa cgc 883
Pro Gly Ser Lys Pro Ile Val Thr Asn Cys Gly Ile Asp Arg Lys Arg
250 255 260
gcc acc ggc ctc aac aaa ttt ggc gac atg tgg atg cac gtt tcc aac 931
Ala Thr Gly Leu Asn Lys Phe Gly Asp Met Trp Met His Val Ser Asn
265 270 275
ggc ctc ggc acc tca aaa ttc gtc cca ttc cgc atc ttc tgc cga ccc 979
Gly Leu Gly Thr Ser Lys Phe Val Pro Phe Arg Ile Phe Cys Arg Pro
280 285 290
agc gcc acc cta ata aag atc act gaa cag gca ctt tgacctgaaa 1025
Ser Ala Thr Leu Ile Lys Ile Thr Glu Gln Ala Leu
295 300 305
aacccacagg actacagtga 1045
<210>74
<211>305
<212>PRT
<213>谷氨酸棒状杆菌
<400>74
Val Asn Lys Val Ser Thr Ile Ala Lys Thr Leu Thr Phe Ser Ser Ala
1 5 10 15
Ala Leu Leu Gly Ala Gly Ile Ala Thr Ala Ala Trp Gly Tyr Ser Glu
20 25 30
Leu Lys Lys Phe Glu Leu Lys Thr Val Glu Leu Pro Ile Leu Lys Pro
35 40 45
Gly Thr Leu Arg Gly Lys Lys Glu Phe Arg Leu Leu His Ile Ser Asp
50 55 60
Leu His Met Ile Pro Gly Gln Glu Thr Lys Lys Ala Trp Val Ser Ala
65 70 75 80
Leu Asp Ser Leu Ser Pro Asp Leu Val Ile Asn Thr Gly Asp Asn Leu
85 90 95
Ser Asp Glu Lys Ala Val Pro Asp Val Leu Arg Ala Leu Gly Pro Leu
100 105 110
MetA sn Arg Pro Gly Ala Phe Val Phe Gly Thr Asn Asp Tyr Trp Ala
115 120 125
Pro Arg Pro Val Asn Pro Phe Gly Tyr Leu Phe Gly Lys Lys Arg Glu
130 135 140
Val Ser His Ile Asp Leu Pro Trp Arg Ala Met Arg Ala Ala Phe Ile
145 150 155 160
Glu His Gly Trp Gln Asp Ala Asn Gln Lys Arg Leu Glu Phe Gln Val
165 170 175
Gly Ser Val Arg Leu Ala Ile Ser Gly Val Asp Asp Pro His His Asp
180 185 190
Leu Asp Asp Tyr Thr Glu Ile Ala Gly Ala Pro Asn Val Asp Ala Asp
195 200 205
Leu Ser Ile Ala Leu Leu His Ala Pro Glu Pro Arg Val Leu Ala Gln
210 215 220
Phe Glu Ala Asp Gly Tyr Gln Leu Ser Leu Ser Gly His Thr His Gly
225 230 235 240
Gly Gln Leu Cys Leu Pro Gly Ser Lys Pro Ile Val Thr Asn Cys Gly
245 250 255
Ile Asp Arg Lys Arg Ala Thr Gly Leu Asn Lys Phe Gly Asp Met Trp
260 265 270
Met His Val Ser Asn Gly Leu Gly Thr Ser Lys Phe Val Pro Phe Arg
275 280 285
Ile Phe Cys Arg Pro Ser Ala Thr Leu Ile Lys Ile Thr Glu Gln Ala
290 295 300
Leu
305
<210>75
<211>883
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(853)
<223>RXA00637
<400>75
ccgatggcac tttccgtacc tggaaggtag ttgcccgcgg cagaaacaat tagggaaacc 60
tcttgacctt cacgcgacgt caaggattta actaacagtc atg aca gat cga acc 115
Met Thr Asp Arg Thr
1 5
att ggc gag gct gct gaa cta ctc ggt gtc acc acc cgc aca ctg cgg 163
Ile Gly Glu Ala Ala Glu Leu Leu Gly Val Thr Thr Arg Thr Leu Arg
10 15 20
cat tgg gac agc atc ggg ttg ctt caa ccg agc tgg cga acc aca acg 211
His Trp Asp Ser Ile Gly Leu Leu Gln Pro Ser Trp Arg Thr Thr Thr
25 30 35
gat tat cgc ctt tac aca gaa gac gat gtc gag cgc gcc ctg cag att 259
Asp Tyr Arg Leu Tyr Thr Glu Asp Asp Val Glu Arg Ala Leu Gln Ile
40 45 50
ctt att tac agg gcc gcc gga atc ggg ttg aag gat att gcg gag gtg 307
Leu Ile Tyr Arg Ala Ala Gly Ile Gly Leu Lys Asp Ile Ala Glu Val
55 60 65
ctt gat cag ccg gat tcg gcc aat cag cac ctg cgc cgc cag cgg gaa 355
Leu Asp Gln Pro Asp Ser Ala Asn Gln His Leu Arg Arg Gln Arg Glu
70 75 80 85
ctc cta gtg gaa cag atc ggt cag ctg cat cgg atg gtc cgg gca gta 403
Leu Leu Val Glu Gln Ile Gly Gln Leu His Arg Met Val Arg Ala Val
90 95 100
gat gaa atc ctt gga aag gat gcg att agc gtg aag gaa aag att gag 451
Asp Glu Ile Leu Gly Lys Asp Ala Ile Ser Val Lys Glu Lys Ile Glu
105 110 115
atc ttc ggc gag gat ctg ccc aag tac caa gag gag gct ttc cag cgc 499
Ile Phe Gly Glu Asp Leu Pro Lys Tyr Gln Glu Glu Ala Phe Gln Arg
120 125 130
tgg ggc gac acc ccg gag tgg aaa gaa tcc caa aag atc cag gag aag 547
Trp Gly Asp Thr Pro Glu Trp Lys Glu Ser Gln Lys Ile Gln Glu Lys
135 140 145
atg acc aag gag gat ttt cag agg gcg aaa gat gaa cat gag ggg ttc 595
Met Thr Lys Glu Asp Phe Gln Arg Ala Lys Asp Glu His Glu Gly Phe
150 155 160 165
gtc gaa aag ctt atc gac gcc tcc ctg cgc ggc atc gcg ccc ggc tct 643
Val Glu Lys Leu Ile Asp Ala Ser Leu Arg Gly Ile Ala Pro Gly Ser
170 175 180
gca gag ggc aat gag cta gcg ctt gcg cac cgc gca agc atc ggt cag 691
Ala Glu Gly Asn Glu Leu Ala Leu Ala His Arg Ala Ser Ile Gly Gln
185 190 195
tgg tac acc gtg agt gca aac aag caa gta att ttg gct cgg atg tac 739
Trp Tyr Thr Val Ser Ala Asn Lys Gln Val Ile Leu Ala Arg Met Tyr
200 205 210
gtg gaa gac gag cgt ttt aac gaa act tac aaa gga cac gcc tca tat 787
Val Glu Asp Glu Arg Phe Asn Glu Thr Tyr Lys Gly His Ala Ser Tyr
215 220 225
ctt tta aca ctt att gaa gca ttg gcg cag gta gaa ggc gtg gac ttg 835
Leu Leu Thr Leu Ile Glu Ala Leu Ala Gln Val Glu Gly Val Asp Leu
230 235 240 245
gag aat gtg gag tgg gaa taatcccaat ttcaactctt tcagtacatt 883
Glu Asn Val Glu Trp Glu
250
<210>76
<211>251
<212>PRT
<213>谷氨酸棒状杆菌
<400>76
Met Thr Asp Arg Thr Ile Gly Glu Ala Ala Glu Leu Leu Gly Val Thr
1 5 10 15
Thr Arg Thr Leu Arg His Trp Asp Ser Ile Gly Leu Leu Gln Pro Ser
20 25 30
Trp Arg Thr Thr Thr Asp Tyr Arg Leu Tyr Thr Glu Asp Asp Val Glu
35 40 45
Arg Ala Leu Gln Ile Leu Ile Tyr Arg Ala Ala Gly Ile Gly Leu Lys
50 55 60
Asp Ile Ala Glu Val Leu Asp Gln Pro Asp Ser Ala Asn Gln His Leu
65 70 75 80
Arg Arg Gln Arg Glu Leu Leu Val Glu Gln Ile Gly Gln Leu His Arg
85 90 95
Met Val Arg Ala Val Asp Glu Ile Leu Gly Lys Asp Ala Ile Ser Val
100 105 110
Lys Glu Lys Ile Glu Ile Phe Gly Glu Asp Leu Pro Lys Tyr Gln Glu
115 120 125
Glu Ala Phe Gln Arg Trp Gly Asp Thr Pro Glu Trp Lys Glu Ser Gln
130 135 140
Lys Ile Gln Glu Lys Met Thr Lys Glu Asp Phe Gln Arg Ala Lys Asp
145 150 155 160
Glu His Glu Gly Phe Val Glu Lys Leu Ile Asp Ala Ser Leu Arg Gly
165 170 175
Ile Ala Pro Gly Ser Ala Glu Gly Asn Glu Leu Ala Leu Ala His Arg
180 185 190
Ala Ser Ile Gly Gln Trp Tyr Thr Val Ser Ala Asn Lys Gln Val Ile
195 200 205
Leu Ala Arg Met Tyr Val Glu Asp Glu Arg Phe Asn Glu Thr Tyr Lys
210 215 220
Gly His Ala Ser Tyr Leu Leu Thr Leu Ile Glu Ala Leu Ala Gln Val
225 230 235 240
Glu Gly Val Asp Leu Glu Asn Val Glu Trp Glu
245 250
<210>77
<21l>1432
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1402)
<223>RXA00654
<400>77
gagatcaatt catatctcaa acattgtaca tctgatcagc aatagcgatt attgcttgat 60
tgtgtgtttt tagatcttcg gttctcttca ctcaactgct gtg aag tgc cac ctg 115
Val Lys Cys His Leu
1 5
ttt gga aag gcg aac acg ata gtg ctc gat att ttg att tac ccg gtg 163
Phe Gly Lys Ala Asn Thr Ile Val Leu Asp Ile Leu Ile Tyr Pro Val
10 15 20
tct gga gtg atg aag ctg tgg cac ctg ctt ctt cac aac gtt gcg ggt 211
Ser Gly Val Met Lys Leu Trp His Leu Leu Leu His Asn Val Ala Gly
25 30 35
ttg gac gat tca ctg gcg tgg ttc ttt tcc ctt ttc ggc ctt gtc atc 259
Leu Asp Asp Ser Leu Ala Trp Phe Phe Ser Leu Phe Gly Leu Val Ile
40 45 50
acg atc cgt gca att atc gcg cct ttc acc tgg cag atg tat aag tcg 307
Thr Ile Arg Ala Ile Ile Ala Pro Phe Thr Trp Gln Met Tyr Lys Set
55 60 65
ggc cgc act gcc gca cat att cgt cct cac cgc gct gcg ctc cgg gaa 355
Gly Arg Thr Ala Ala His Ile Arg Pro His Arg Ala Ala Leu Arg Glu
70 75 80 85
gaa tac aag gga aag tac gat gaa gcg tcc att cgg gag ttg cag aag 403
Glu Tyr Lys Gly Lys Tyr Asp Glu Ala Ser Ile Arg Glu Leu Gln Lys
90 95 100
cgc cag aat gat ttg aat aag gaa tac ggc att aac ccg ctg gca ggt 451
Arg Gln Asn Asp Leu Asn Lys Glu Tyr Gly Ile Asn Pro Leu Ala Gly
105 110 115
tgt gtg cct ggg ctg atc cag ata ccg att gtc ctt ggt ctt tac tgg 499
Cys Val Pro Gly Leu Ile Gln Ile Pro Ile Val Leu Gly Leu Tyr Trp
120 125 130
gca ctt ctc cgc atg gct cgc cct gaa ggt ggt ttg gaa aat ccc gtc 547
Ala Leu Leu Arg Met Ala Arg Pro Glu Gly Gly Leu Glu Asn Pro Val
135 140 145
ttc cag tcg atc ggc ttc cta act cct gag gaa gtg gaa tct ttc ctc 595
Phe Gln Ser Ile Gly Phe Leu Thr Pro Glu Glu Val Glu Ser Phe Leu
150 155 160 165
gct ggt cgc gtg agc aat gtg cct ctg ccc gct tat gtt tcg atg ccc 643
Ala Gly Arg Val Ser Asn Val Pro Leu Pro Ala Tyr Val Ser Met Pro
170 175 180
act gag cag cta aaa tat ttg agc acc acg cag gcg gaa gtt ctt agt 691
Thr Glu Gln Leu Lys Tyr Leu Ser Thr Thr Gln Ala Glu Val Leu Ser
185 190 195
ttc gtt ttg cca ctg ttc atc aca gcc gca atc ctc acc gca atc aac 739
Phe Val Leu Pro Leu Phe Ile Thr Ala Ala Ile Leu Thr Ala Ile Asn
200 205 210
atg gcg atg tcc atg tac cgc agc ttc caa acc aac gat tac gca tcc 787
Met Ala Met Ser Met Tyr Arg Ser Phe Gln Thr Asn Asp Tyr Ala Ser
215 220 225
gga ttc tct aac ggc atg ctg aag ttc atg atc gtg atg tcg atc ctc 835
Gly Phe Ser Asn Gly Met Leu Lys Phe Met Ile Val Met Ser Ile Leu
230 235 240 245
gcg ccg atc ttc cca ctg tcc ctt ggc ctc aca gga cca ttc ccc aca 883
Ala Pro Ile Phe Pro Leu Ser Leu Gly Leu Thr Gly Pro Phe Pro Thr
250 255 260
gca atc gca ctc tat tgg gtc agc aac aac ctg tgg acg ctc ctc caa 931
Ala Ile Ala Leu Tyr Trp Val Ser Asn Asn Leu Trp Thr Leu Leu Gln
265 270 275
aca atc atc atg atg gtc att ttg gaa cgc aaa tac cca ctt acc gac 979
Thr Ile Ile Met Met Val Ile Leu Glu Arg Lys Tyr Pro Leu Thr Asp
280 285 290
gat ttc aaa gtg cac cac cta gag cag cgc gac atc tac cgc gca aaa 1027
Asp Phe Lys Val His His Leu Glu Gln Arg Asp Ile Tyr Arg Ala Lys
295 300 305
caa aaa gaa aag cgc atc ttc ctg tgg aca cga cgc aaa aac cgc gcc 1075
Gln Lys Glu Lys Arg Ile Phe Leu Trp Thr Arg Arg Lys Asn Arg Ala
3 0 315 320 325
ctg atg att ctc acc cca tgg aac gcc tca acg ctt cac gca aca aac 1123
Leu Met Ile Leu Thr Pro Trp Asn Ala Ser Thr Leu His Ala Thr Asn
330 335 340
gtg gaa ctc acc aaa acc cgt act gcc gaa atc aac gaa gca aaa cag 1171
Val Glu Leu Thr Lys Thr Arg Thr Ala Glu Ile Asn Glu Ala Lys Gln
345 350 355
gcc cgc aaa gaa atc gcg aac aag agg cgc gaa acg caa cgt gaa atg 1219
Ala Arg Lys Glu Ile Ala Asn Lys Arg Arg Glu Thr Gln Arg Glu Met
360 365 370
aac cgc gcc gcc atg cag cgc tta aag cag cgt cgc gct gag gtt aaa 1267
Asn Arg Ala Ala Met Gln Arg Leu Lys Gln Arg Arg Ala Glu Val Lys
375 380 385
gct aaa aag aag ggg ctt atc gac gcc tcc ccc aac gaa gat acc cct 1315
Ala Lys Lys Lys Gly Leu Ile Asp Ala Ser Pro Asn Glu Asp Thr Pro
390 395 400 405
tcg gaa aat gaa gaa act aaa ttg agt agt ccg cag gtg gag ccg aca 1363
Ser Glu Asn Glu Glu Thr Lys Leu Ser Ser Pro Gln Val Glu Pro Thr
410 415 420
aca act gcc gag cca aat cgc gag ccg tct caa gag gac tgatgttgtg 1412
Thr Thr Ala Glu Pro Asn Arg Glu Pro Ser Gln Glu Asp
425 430
gaccaatcga gatccagcaa 1432
<210>78
<211>434
<212>PRT
<213>谷氨酸棒状杆菌
<400>78
Val Lys Cys His Leu Phe Gly Lys Ala Asn Thr Ile Val Leu Asp Ile
1 5 l0 15
Leu Ile Tyr Pro Val Ser Gly Val Met Lys Leu Trp His Leu Leu Leu
20 25 30
His Asn Val Ala Gly Leu Asp Asp Ser Leu Ala Trp Phe Phe Ser Leu
35 40 45
Phe Gly Leu Val Ile Thr Ile Arg Ala Ile Ile Ala Pro Phe Thr Trp
50 55 60
Gln Met Tyr Lys Ser Gly Arg Thr Ala Ala His Ile Arg Pro His Arg
65 70 75 80
Ala Ala Leu Arg Glu Glu Tyr Lys Gly Lys Tyr Asp Glu Ala Ser Ile
85 90 95
Arg Glu Leu Gln Lys Arg Gln Asn Asp Leu Asn Lys Glu Tyr Gly Ile
100 105 110
Asn Pro Leu Ala Gly Cys Val Pro Gly Leu Ile Gln Ile Pro Ile Val
115 120 125
Leu Gly Leu Tyr Trp Ala Leu Leu Arg Met Ala Arg Pro Glu Gly Gly
130 135 140
Leu Glu Asn Pro Val Phe Gln Ser Ile Gly Phe Leu Thr Pro Glu Glu
145 150 155 160
Val Glu Ser Phe Leu Ala Gly Arg Val Ser Asn Val Pro Leu Pro Ala
165 170 175
Tyr Val Ser Met Pro Thr Glu Gln Leu Lys Tyr Leu Ser Thr Thr Gln
180 185 190
Ala Glu Val Leu Ser Phe Val Leu Pro Leu Phe Ile Thr Ala Ala Ile
195 200 205
Leu Thr Ala Ile Asn Met Ala Met Ser Met Tyr Arg Ser Phe Gln Thr
210 215 220
Asn Asp Tyr Ala Ser Gly Phe Ser Asn Gly Met Leu Lys Phe Met Ile
225 230 235 240
Val Met Ser Ile Leu Ala Pro Ile Phe Pro Leu Ser Leu Gly Leu Thr
245 250 255
Gly Pro Phe Pro Thr Ala Ile Ala Leu Tyr Trp Val Ser Asn Asn Leu
260 265 270
Trp Thr Leu Leu Gln Thr Ile Ile Met Met Val Ile Leu Glu Arg Lys
275 280 285
Tyr Pro Leu Thr Asp Asp Phe Lys Val His His Leu Glu Gln Arg Asp
290 295 300
Ile Tyr Arg Ala Lys Gln Lys Glu Lys Arg Ile Phe Leu Trp Thr Arg
305 310 315 320
Arg Lys Asn Arg Ala Leu Met Ile Leu Thr Pro Trp Asn Ala Ser Thr
325 330 335
Leu His Ala Thr Asn Val Glu Leu Thr Lys Thr Arg Thr Ala Glu Ile
340 345 350
Asn Glu Ala Lys Gln Ala Arg Lys Glu Ile Ala Asn Lys Arg Arg Glu
355 360 365
Thr Gln Arg Glu Met Asn Arg Ala Ala Met Gln Arg Leu Lys Gln Arg
370 375 380
Arg Ala Glu Val Lys Ala Lys Lys Lys Gly Leu Ile Asp Ala Ser Pro
385 390 395 400
Asn Glu Asp Thr Pro Ser Glu Asn Glu Glu Thr Lys Leu Ser Ser Pro
405 410 415
Gln Val Glu Pro Thr Thr Thr Ala Glu Pro Asn Arg Glu Pro Ser Gln
420 425 430
Glu Asp
<210>79
<211>865
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(835)
<223>RXA00678
<400>79
tgcgcagtgc actttccagg aaccaaaata tccccgccga ccttgctgca gatatttcgc 60
agccgctgaa aaaactagcc gttgacctgg aggttacccg atg act cgc agt aat 115
Met Thr Arg Ser Asn
1 5
tta ccc gct tgg gag caa gca gac ccc agc gtc cac gcc tct gat ccc 163
Leu Pro Ala Trp Glu Gln Ala Asp Pro Ser Val His Ala Ser Asp Pro
10 15 20
cgc gcc atc act ttt gcg gag gat ttt ggg att cgt ccc atc ccg gcc 211
Arg Ala Ile Thr Phe Ala Glu Asp Phe Gly Ile Arg Pro Ile Pro Ala
25 30 35
gta ggt cca att gat acc acc gct atc tgt gca aca cct tca aat gga 259
Val Gly Pro Ile Asp Thr Thr Ala Ile Cys Ala Thr Pro Ser Asn Gly
40 45 50
ttt gag caa tta tgg aaa gcc att gag ccg gaa acc cgc acc cgc gcc 307
Phe Glu Gln Leu Trp Lys Ala Ile Glu Pro Glu Thr Arg Thr Arg Ala
55 60 65
aac gac att cat ctt ccc atc gtc gtg gcg tac gct gaa cgt ctc tgc 355
Asn Asp Ile His Leu Pro Ile Val Val Ala Tyr Ala Glu Arg Leu Cys
70 75 80 85
gac gct tac cca ctg gca gat aga gaa ttg gtt ctc gta gcc gca atc 403
Asp Ala Tyr Pro Leu Ala Asp Arg Glu Leu Val Leu Val Ala Ala Ile
90 95 100
ctc cac gac acg ggc tgg gca cat gtc gat gag agc cga atc atc tcc 451
Leu His Asp Thr Gly Trp Ala His Val Asp Glu Ser Arg Ile Ile Ser
105 110 115
gaa gga ttc tcc gga aac tgg cgc aaa gca gca atc cgc ttc gaa cac 499
Glu Gly Phe Ser Gly Asn Trp Arg Lys Ala Ala Ile Arg Phe Glu His
120 125 130
gaa aca gaa ggc tgc act gtg gcc cgc cga gtg ctt cca tcc ctc gga 547
Glu Thr Glu Gly Cys Thr Val Ala Arg Arg Val Leu Pro Ser Leu Gly
135 140 145
tat act gtc gat ttt gtc gag cat gtc tgc gac atc atc gac gga cac 595
Tyr Thr Val Asp Phe Val Glu His Val Cys Asp Ile Ile Asp Gly His
150 155 160 165
gac acc cgc caa gtg gcc tac tca cta gaa gac gct ctt gtt cgc gat 643
Asp Thr Arg Gln Val Ala Tyr Ser Leu Glu Asp Ala Leu Val Arg Asp
170 175 180
tgc gac cga ctc tgg cgt ttc gac cgc gcc ggg atc acg gct tct agc 691
Cys Asp Arg Leu Trp Arg Phe Asp Arg Ala Gly Ile Thr Ala Ser Ser
185 190 195
tca tgg ttc ggg atg cca gtc tca gat tat gtc gac cgc ctg cac agg 739
Ser Trp Phe Gly Met Pro Val Ser Asp Tyr Val Asp Arg Leu His Arg
200 205 210
gaa atc ctc cca gaa tta atc acc gaa gct gca cac cag atg gct act 787
Glu Ile Leu Pro Glu Leu Ile Thr Glu Ala Ala His Gln Met Ala Thr
215 220 225
gca gac ctc aat cgt gca aag gcc ctg tta aga acg gat gcg atc cga 835
Ala Asp Leu Asn Arg Ala Lys Ala Leu Leu Arg Thr Asp Ala Ile Arg
230 235 240 245
tgaatgctgc aaccaggcgtgcttctctgc 865
<210>80
<211>245
<212>PRT
<213>谷氨酸棒状杆菌
<400>80
Met Thr Arg Ser Asn Leu Pro Ala Trp Glu Gln Ala Asp Pro Ser Val
1 5 10 15
His Ala Ser Asp Pro Arg Ala Ile Thr Phe Ala Glu Asp Phe Gly Ile
20 25 30
Arg Pro Ile Pro Ala Val Gly Pro Ile Asp Thr Thr Ala Ile Cys Ala
35 40 45
Thr Pro Ser Asn Gly Phe Glu Gln Leu Trp Lys Ala Ile Glu Pro Glu
50 55 60
Thr Arg Thr Arg Ala Asn Asp Ile His Leu Pro Ile Val Val Ala Tyr
65 70 75 80
Ala Glu Arg Leu Cys Asp Ala Tyr Pro Leu Ala Asp Arg Glu Leu Val
85 90 95
Leu Val Ala Ala Ile Leu His Asp Thr Gly Trp Ala His Val Asp Glu
100 105 110
Ser Arg Ile Ile Ser Glu Gly Phe Ser Gly Asn Trp Arg Lys Ala Ala
115 120 125
Ile Arg Phe Glu His Glu Thr Glu Gly Cys Thr Val Ala Arg Arg Val
130 135 140
Leu Pro Ser Leu Gly Tyr Thr Val Asp Phe Val Glu His Val Cys Asp
145 150 155 160
Ile Ile Asp Gly His Asp Thr Arg Gln Val Ala Tyr Ser Leu Glu Asp
165 170 175
Ala Leu Val Arg Asp Cys Asp Arg Leu Trp Arg Phe Asp Arg Ala Gly
180 185 190
Ile Thr Ala Ser Ser Ser Trp Phe Gly Met Pro Val Ser Asp Tyr Val
195 200 205
Asp Arg Leu His Arg Glu Ile Leu Pro Glu Leu Ile Thr Glu Ala Ala
210 215 220
His Gln Met Ala Thr Ala Asp Leu Asn Arg Ala Lys Ala Leu Leu Arg
225 230 235 240
Thr Asp Ala Ile Arg
245
<210>81
<211>1201
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1171)
<223>RXA00691
<400>81
gaattatcat cgccatcttg gccatccttg ccgccatcgc tggtgtcatg ggaccaatga 60
tggctaacct gcagttttaa catctgagag aagtacagtg atg tct cga acc tca 115
Met Ser Arg Thr Ser
1 5
act ttt cac ctg tcg ctg aag tct ttt gca gct gca gtc gca cta tgg 163
Thr Phe His Leu Ser Leu Lys Ser Phe Ala Ala Ala Val Ala Leu Trp
10 15 20
att gtt ctt gcc ata tcg cca tgg gtt cct tct gcg cac gcc ttg acc 211
Ile Val Leu Ala Ile Ser Pro Trp Val Pro Ser Ala His Ala Leu Thr
25 30 35
scg cct tgt gaa gsc gac caa gtg acs gtg atg gtt gag ggc aac acc 259
Thr Pro Cys Glu Asp Asp Gln Val Thr Val Met Val Glu Gly Asn Thr
40 45 50
atc ggc tgt gct gac gcc ggc gga aat ggc tat caa acg ctt ctt gat 307
Ile Gly Cys Ala Asp Ala Gly Gly Asn Gly Tyr Gln Thr Leu Leu Asp
55 60 65
gcc gga ttt gat gtg gaa acc acc gtt cag ttt cca gaa ttc ttg tgc 355
Ala Gly Phe Asp Val Glu Thr Thr Val Gln Phe Pro Glu Phe Leu Cys
70 75 80 85
cga atc aat gat ttc cca ggt cct gat gtg gac gat tgc atg acc gca 403
Arg Ile Asn Asp Phe Pro Gly Pro Asp Val Asp Asp Cys Met Thr Ala
90 95 100
tca cct gct gaa gct tat tgg tct tat tgg cat gct ccc ctc ggc gga 451
Ser Pro Ala Glu Ala Tyr Trp Ser Tyr Trp His Ala Pro Leu Gly Gly
105 110 115
gat gaa tgg gaa tac agc aac ctt gga gct ttc ctc tac tac ccc aaa 499
Asp Glu Trp Glu Tyr Ser Asn Leu Gly Ala Phe Leu Tyr Tyr Pro Lys
120 125 130
ccc ggc act gtt gaa gcg tgg tat tgg gga gat act gat cgt ccc ggc 547
Pro Gly Thr Val Glu Ala Trp Tyr Trp Gly Asp Thr Asp Arg Pro Gly
135 140 145
gcg atc ccg gtg agt aaa tct cag gcg gag ttg ggg tta gac tcc gcg 595
Ala Ile Pro Val Ser Lys Ser Gln Ala Glu Leu Gly Leu Asp Ser Ala
150 155 160 165
gat ccg gat tac aaa att gat ttt gat cca aat gat ttc atc acc aca 643
Asp Pro Asp Tyr Lys Ile Asp Phe Asp Pro Asn Asp Phe Ile Thr Thr
170 175 180
acc cca acc tcc gag tcc cct atc gcc gat tta ggg ggt gac gaa gag 691
Thr Pro Thr Ser Glu Ser Pro Ile Ala Asp Leu Gly Gly Asp Glu Glu
185 190 195
ccc act gaa act act gaa gct cct atc gca ggg gct gga gct ggt tcg 739
Pro Thr Glu Thr Thr Glu Ala Pro Ile Ala Gly Ala Gly Ala Gly Ser
200 205 210
gca aga gga caa gca gct gaa ccc act gct gag gta sac cct gag aac 787
Ala Arg Gly Gln Ala Ala Glu Pro Thr Ala Glu Val Asn Pro Glu Asn
215 220 225
cct aat gag gta ttg gtg tat cag gat tct gaa gga aac tcg att act 835
Pro Asn Glu Val Leu Val Tyr Gln Asp Ser Glu Gly Asn Ser Ile Thr
230 235 240 245
aaa ggt cag tac gag aac ttg gtt gct gca gca gcc gct aag acc act 883
Lys Gly Gln Tyr Glu Asn Leu Val Ala Ala Ala Ala Ala Lys Thr Thr
250 255 260
gct gct gtc caa gcc cca gct ggg gct ggt gaa gca aat agc caa ccg 931
Ala Ala Val Gln Ala Pro Ala Gly Ala Gly Glu Ala Asn Ser Gln Pro
265 270 275
cag gca aca gcg gtg gca gaa gct cct gaa gtt gat ccg atg acc acg 979
Gln Ala Thr Ala Val Ala Glu Ala Pro Glu Val Asp Pro Met Thr Thr
280 285 290
cag gtt ctt atg gcg cct gcg ggc caa gat gga gac gtc atg gct gag 1027
Gln Val Leu Met Ala Pro Ala Gly Gln Asp Gly Asp Val Met Ala Glu
295 300 305
ggt tcg acg cag cag acg tac gcc aca ggc acg gtg gat tct tct gct 1075
Gly Ser Thr Gln Gln Thr Tyr Ala Thr Gly Thr Val Asp Ser Ser Ala
310 315 320 325
cag gga tgg atc att gga ctc aca ctg gct gtc att tcc ttg gtt tca 1123
Gln Gly Trp Ile Ile Gly Leu Thr Leu Ala Val Ile Ser Leu Val Ser
330 335 340
gcg tct gct gtg gcg gcg tgg gcg att cgt cgt tca gag gtc cag ggt 1171
Ala Ser Ala Val Ala Ala Trp Ala Ile Arg Arg Ser Glu Val Gln Gly
345 350 355
taaagcttcg tgaaatggat tgagcgatat 1201
<210>82
<211>357
<212>PRT
<213>谷氨酸棒状杆菌
<400>82
Met Ser Arg Thr Ser Thr Phe His Leu Ser Leu Lys Ser Phe Ala Ala
1 5 10 15
Ala Val Ala Leu Trp Ile Val Leu Ala Ile Ser Pro Trp Val Pro Ser
20 25 30
Ala His Ala Leu Thr Thr Pro Cys Glu Asp Asp G1n Val Thr Val Met
35 40 45
Val Glu Gly Asn Thr Ile Gly Cys Ala Asp Ala Gly Gly Asn Gly Tyr
50 55 60
Gln Thr Leu Leu Asp Ala Gly Phe Asp Val Glu Thr Thr Val Gln Phe
65 70 75 80
Pro Glu Phe Leu Cys Arg Ile Asn Asp Phe Pro Gly Pro Asp Val Asp
85 90 95
Asp Cys Met Thr Ala Ser Pro Ala Glu Ala Tyr Trp Ser Tyr Trp His
100 105 110
Ala Pro Leu Gly Gly Asp Glu Trp Glu Tyr Ser Asn Leu Gly Ala Phe
115 120 125
Leu Tyr Tyr Pro Lys Pro Gly Thr Val Glu Ala Trp Tyr Trp Gly Asp
130 135 140
Thr Asp Arg Pro Gly Ala Ile Pro Val Ser Lys Ser Gln Ala Glu Leu
145 150 155 160
Gly Leu Asp Ser Ala Asp Pro Asp Tyr Lys Ile Asp Phe Asp Pro Asn
165 170 175
Asp Phe Ile Thr Thr Thr Pro Thr Ser Glu Ser Pro Ile Ala Asp Leu
180 185 190
Gly Gly Asp Glu Glu Pro Thr Glu Thr Thr Glu Ala Pro Ile Ala Gly
195 200 205
Ala Gly Ala Gly Ser Ala Arg Gly Gln Ala Ala Glu Pro Thr Ala Glu
210 215 220
Val Asn Pro Glu Asn Pro Asn Glu Val Leu Val Tyr Gln Asp Ser Glu
225 230 235 240
Gly Asn Ser Ile Thr Lys Gly Gln Tyr Glu Asn Leu Val Ala Ala Ala
245 250 255
Ala Ala Lys Thr Thr Ala Ala Val Gln Ala Pro Ala Gly Ala Gly Glu
260 265 270
Ala Asn Ser Gln Pro Gln Ala Thr Ala Val Ala Glu Ala Pro Glu Val
275 280 285
Asp Pro Met Thr Thr Gln Val Leu Met Ala Pro Ala Gly Gln Asp Gly
290 295 300
Asp Val Met Ala Glu Gly Ser Thr Gln Gln Thr Tyr Ala Thr Gly Thr
305 310 315 320
Val Asp Ser Ser Ala Gln Gly Trp Ile Ile Gly Leu Thr Leu Ala Val
325 330 335
Ile Ser Leu Val Ser Ala Ser Ala Val Ala Ala Trp Ala Ile Arg Arg
340 345 350
Ser Glu Val Gln Gly
355
<210>83
<211>1759
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1729)
<223>RXA00719
<400>83
cagatgatgc acacatcgtg gacacctctg atatgaccat ggatcaagta cttgatcacc 60
tcatccacct agtggaagcc tccgctgaaa ggagcaacca gtg act gat aaa cac 115
Val Thr Asp Lys His
1 5
acc atg cct ggt gaa gag gac gac acc gta ttc gtc tac cac acc cac 163
Thr Met Pro Gly Glu Glu Asp Asp Thr Val Phe Val Tyr His Thr His
10 15 20
aaa ggc gaa atg gac gtc gaa ggt gcg ttt gct gac gaa gaa gaa cta 211
Lys Gly Glu Met Asp Val Glu Gly Ala Phe Ala Asp Glu Glu Glu Leu
25 30 35
gca cca cac ggc ggt tgg gct tcc gca gat ttc gac cca gca gaa ttc 259
Ala Pro His Gly Gly Trp Ala Ser Ala Asp Phe Asp Pro Ala Glu Phe
40 45 50
ggc tac gaa gac tct gac gat gac ttc gat gca gag gac ttt gac gaa 307
Gly Tyr Glu Asp Ser Asp Asp Asp Phe Asp Ala Glu Asp Phe Asp Glu
55 60 65
aca gag ttc tcc aac cct gat ttc ggc gaa gac tac tct gat gaa gac 355
Thr Glu Phe Ser Asn Pro Asp Phe Gly Glu Asp Tyr Ser Asp Glu Asp
70 75 80 85
tgg gaa gaa atc gag acc gca ttc gga ttc gac cca agc cac ctt gaa 403
Trp Glu Glu Ile Glu Thr Ala Phe Gly Phe Asp Pro Ser His Leu Glu
90 95 100
gaa gct ctc tgc acg gtc gct atc gtc gga cgc cca aat gtt ggt aaa 451
Glu Ala Leu Cys Thr Val Ala Ile Val Gly Arg Pro Asn Val Gly Lys
105 110 115
tca acc ttg gtg aac cgc ttt att gga cgt cga gaa gca gtc gtg gaa 499
Ser Thr Leu Val Asn Arg Phe Ile Gly Arg Arg Glu Ala Val Val Glu
120 125 130
gat ttc ccc ggc gta acc cgt gac cgc atc tcc tac atc tct gac tgg 547
Asp Phe Pro Gly Val Thr Arg Asp Arg Ile Ser Tyr Ile Ser Asp Trp
135 140 145
ggt gga cac cgt ttc tgg gtt cag gac aca ggc gga tgg gat cct aac 595
Gly Gly His Arg Phe Trp Val Gln Asp Thr Gly Gly Trp Asp Pro Asn
150 155 160 165
gtc aag ggc atc cac gca tcg atc gca cag caa gca gaa gtt gct atg 643
Val Lys Gly Ile His Ala Ser Ile Ala Gln Gln Ala Glu Val Ala Met
170 175 180
agc act gcc gat gtc atc gta ttc gtc gtg gac acc aag gtg ggc atc 691
Ser Thr Ala Asp Val Ile Val Phe Val Val Asp Thr Lys Val Gly Ile
185 190 195
acc gaa act gac tca gtg atg gca gca aaa ctg ttg cgc tcg gaa gtg 739
Thr Glu Thr Asp Ser Val Met Ala Ala Lys Leu Leu Arg Ser Glu Val
200 205 210
cca gtg atc ttg gtt gcg aac aaa ttc gac tcc gac agc cag tgg gct 787
Pro Val Ile Leu Val Ala Asn Lys Phe Asp Ser Asp Ser Gln Trp Ala
215 220 225
gac atg gct gag ttc tac agc ctc ggc ctt ggc gat cca tac cca gtt 835
Asp Met Ala Glu Phe Tyr Ser Leu Gly Leu Gly Asp Pro Tyr Pro Val
230 235 240 245
tca gcc cag cat gga cgt ggt ggc gct gac gtt ttg gac aaa gtc ctt 883
Ser Ala Gln His Gly Arg Gly Gly Ala Asp Val Leu Asp Lys Val Leu
250 255 260
gaa ctc ttc cca gaa gag cct cgc tcc aag tcc atc gtg gaa ggc cct 931
Glu Leu Phe Pro Glu Glu Pro Arg Ser Lys Ser Ile Val Glu Gly Pro
265 270 275
cgt cgt gtc gcc ctt gtg ggt aag cca aac gtg ggt aag tct tca ctg 979
Arg Arg Val Ala Leu Val Gly Lys Pro Asn Val Gly Lys Ser Ser Leu
280 285 290
ctc aac aag ttt gct ggc gag acc cgc tct gtc gtg gac aat gtt gca 1027
Leu Asn Lys Phe Ala Gly Glu Thr Arg Ser Val Val Asp Asn Val Ala
295 300 305
gga acc acc gtt gac ccc gtt gac tcc ctg att cag ctg gat caa aaa 1075
Gly Thr Thr Val Asp Pro Val Asp Ser Leu Ile Gln Leu Asp Gln Lys
310 315 320 325
ctg tgg aaa ttc gtg gat act gct ggt ctt cgc aaa aag gtc aag act 1123
Leu Trp Lys Phe Val Asp Thr Ala Gly Leu Arg Lys Lys Val Lys Thr
330 335 340
gca tct ggc cac gag tac tac gca tca ctg cgt acc cac ggt gcc atc 1171
Ala Ser Gly His Glu Tyr Tyr Ala Ser Leu Arg Thr His Gly Ala Ile
345 350 355
gat gca gct gag ctg tgt gtt ttg ctt atc gat tcc tcc gaa ccc atc 1219
Asp Ala Ala Glu Leu Cys Val Leu Leu Ile Asp Ser Ser Glu Pro Ile
360 365 370
acc gag cag gat cag cgc gtg ctc gca atg atc acc gat gcc ggt aag 1267
Thr Glu Gln Asp Gln Arg Val Leu Ala Met Ile Thr Asp Ala Gly Lys
375 380 385
gca ctg gtt att gcg ttc aac aag tgg gat ctc atg gat gaa gat cgc 1315
Ala Leu Val Ile Ala Phe Asn Lys Trp Asp Leu Met Asp Glu Asp Arg
390 395 400 405
cgc atc gat ttg gat cgc gaa ctt gat ctc cag ttg gca cac gtg cct 1363
Arg Ile Asp Leu Asp Arg Glu Leu Asp Leu Gln Leu Ala His Val Pro
410 415 420
tgg gca aag cgc atc aac atc tcc gcc aaa acc ggt cgt gca ctg cag 1411
Trp Ala Lys Arg Ile Asn Ile Ser Ala Lys Thr Gly Arg Ala Leu Gln
425 430 435
cgc ctc gag cca gca atg ttg gaa gcg ctc gac aac tgg gat cgc cgt 1459
Arg Leu Glu Pro Ala Met Leu Glu Ala Leu Asp Asn Trp Asp Arg Arg
440 445 450
atc tcc act ggt cag ctg aac acc tgg ctg cgt gaa gca att gct gcg 1507
Ile Ser Thr Gly Gln Leu Asn Thr Trp Leu Arg Glu Ala Ile Ala Ala
455 460 465
aac cca cca cca atg cgt ggc gga cgt ttg cct cga gtg ctg ttt gcc 1555
Asn Pro Pro Pro Met Arg Gly Gly Arg Leu Pro Arg Val Leu Phe Ala
470 475 480 485
acc cag gca tct act cag cca cca gtg atc gta ctg ttc acc acc ggc 1603
Thr Gln Ala Ser Thr Gln Pro Pro Val Ile Val Leu Phe Thr Thr Gly
490 495 500
ttc ctc gaa gca ggt tac cga cga tac ctg gag cgc aag ttc cgt gaa 1651
Phe Leu Glu Ala Gly Tyr Arg Arg Tyr Leu Glu Arg Lys Phe Arg Glu
505 510 515
cgt ttc ggc ttt gaa ggc act cca gtg cga atc gct gtg cgt gtt cgc 1699
Arg Phe Gly Phe Glu Gly Thr Pro Val Arg Ile Ala Val Arg Val Arg
520 525 530
gag cgc cgc ggc aag ggc gga aac aag cag taaagcttga ttttccctaa 1749
Glu Arg Arg Gly Lys Gly Gly Asn Lys Gln
535 540
aagcacttat 1759
<210>84
<211>543
<212>PRT
<213>谷氨酸棒状杆菌
<400>84
Val Thr Asp Lys His Thr Met Pro Gly Glu Glu Asp Asp Thr Val Phe
1 5 10 15
Val Tyr His Thr His Lys Gly Glu Met Asp Val Glu Gly Ala Phe Ala
20 25 30
Asp Glu Glu Glu Leu Ala Pro His Gly Gly Trp Ala Ser Ala Asp Phe
35 40 45
Asp Pro Ala Glu Phe Gly Tyr Glu Asp Ser Asp Asp Asp Phe Asp Ala
50 55 60
Glu Asp Phe Asp Glu Thr Glu Phe Ser Asn Pro Asp Phe Gly Glu Asp
65 70 75 80
Tyr Ser Asp Glu Asp Trp Glu Glu Ile Glu Thr Ala Phe Gly Phe Asp
85 90 95
Pro Ser His Leu Glu Glu Ala Leu Cys Thr Val Ala Ile Val Gly Arg
100 105 110
Pro Asn Val Gly Lys Ser Thr Leu Val Asn Arg Phe Ile Gly Arg Arg
115 120 125
Glu Ala Val Val Glu Asp Phe Pro Gly Val Thr Arg Asp Arg Ile Ser
130 135 140
Tyr Ile Ser Asp Trp Gly Gly His Arg Phe Trp Val Gln Asp Thr Gly
145 150 155 160
Gly Trp Asp Pro Asn Val Lys Gly Ile His Ala Ser Ile Ala Gln Gln
165 170 175
Ala Glu Val Ala Met Ser Thr Ala Asp Val Ile Val Phe Val Val Asp
180 185 190
Thr Lys Val Gly Ile Thr Glu Thr Asp Ser Val Met Ala Ala Lys Leu
195 200 205
Leu Arg Ser Glu Val Pro Val Ile Leu Val Ala Asn Lys Phe Asp Ser
210 215 220
Asp Ser Gln Trp Ala Asp Met Ala Glu Phe Tyr Ser Leu Gly Leu Gly
225 230 235 240
Asp Pro Tyr Pro Val Ser Ala Gln His Gly Arg Gly Gly Ala Asp Val
245 250 255
Leu Asp Lys Val Leu Glu Leu Phe Pro Glu Glu Pro Arg Ser Lys Ser
260 265 270
Ile Val Glu Gly Pro Arg Arg Val Ala Leu Val Gly Lys Pro Asn Val
275 280 285
Gly Lys Ser Ser Leu Leu Asn Lys Phe Ala Gly Glu Thr Arg Ser Val
290 295 300
Val Asp Asn Val Ala Gly Thr Thr Val Asp Pro Val Asp Ser Leu Ile
305 310 315 320
Gln Leu Asp Gln Lys Leu Trp Lys Phe Val Asp Thr Ala Gly Leu Arg
325 330 335
Lys Lys Val Lys Thr Ala Ser Gly His Glu Tyr Tyr Ala Ser Leu Arg
340 345 350
Thr His Gly Ala Ile Asp Ala Ala Glu Leu Cys Val Leu Leu Ile Asp
355 360 365
Ser Ser Glu Pro Ile Thr Glu Gln Asp Gln Arg Val Leu Ala Met Ile
370 375 380
Thr Asp Ala Gly Lys Ala Leu Val Ile Ala Phe Asn Lys Trp Asp Leu
385 390 395 400
Met Asp Glu Asp Arg Arg Ile Asp Leu Asp Arg Glu Leu Asp Leu Gln
405 410 415
Leu Ala His Val Pro Trp Ala Lys Arg Ile Asn Ile Ser Ala Lys Thr
420 425 430
Gly Arg Ala Leu Gln Arg Leu Glu Pro Ala Met Leu Glu Ala Leu Asp
435 440 445
Asn Trp Asp Arg Arg Ile Ser Thr Gly Gln Leu Asn Thr Trp Leu Arg
450 455 460
Glu Ala Ile Ala Ala Asn Pro Pro Pro Met Arg Gly Gly Arg Leu Pro
465 470 475 480
Arg Val Leu Phe Ala Thr Gln Ala Ser Thr Gln Pro Pro Val Ile Val
485 490 495
Leu Phe Thr Thr Gly Phe Leu Glu Ala Gly Tyr Arg Arg Tyr Leu Glu
500 505 510
Arg Lys Phe Arg Glu Arg Phe Gly Phe Glu Gly Thr Pro Val Arg Ile
515 520 525
Ala Val Arg Val Arg Glu Arg Arg Gly Lys Gly Gly Asn Lys Gln
530 535 540
<210>85
<211>2626
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2596)
<223>RXA00731
<400>85
ttgataccgt ggccggtgga cattccttcc aggtgtggcg cgtggcttta gcgaatactt 60
ttgattgggt tgccaagcgc ggcggattgc aggtttaaca atg aaa gac gct tca 115
Met Lys Asp Ala Ser
1 5
cag tcc tac tta tta ttc ggt ctg aga tgg ctg ggc agg tcc ctg cgt 163
Gln Ser Tyr Leu Leu Phe Gly Leu Arg Trp Leu Gly Arg Ser Leu Arg
10 15 20
ttc gca ccg tta agc ctg ctc atg atc gtt gtc atg cgg ggt ttg agg 211
Phe Ala Pro Leu Ser Leu Leu Met Ile Val Val Met Arg Gly Leu Arg
25 30 35
gaa gtg ttt ggc gcc gaa gat ccc gca aat agc tct ttg gta gac aac 259
Glu Val Phe Gly Ala Glu Asp Pro Ala Asn Ser Ser Leu Val Asp Asn
40 45 50
ctt ggg ctc acc ctg ccg tgg tct ctt aat gat ccg cat ttt ctc acc 307
Leu Gly Leu Thr Leu Pro Trp Ser Leu Asn Asp Pro His Phe Leu Thr
55 60 65
gca ggg ttt agc gct tcc acc acc aca gca gcg ctc atg tcc acg ttg 355
Ala Gly Phe Ser Ala Ser Thr Thr Thr Ala Ala Leu Met Ser Thr Leu
70 75 80 85
tgg atc atc gtg ttt gcg gtg ccc tct gaa cgg att ctg ggc agc ctc 403
Trp Ile Ile Val Phe Ala Val Pro Ser Glu Arg Ile Leu Gly Ser Leu
90 95 100
aaa ttc gcg atc aca gca gcg ctt atc cac atc act tcc att ccg ctg 451
Lys Phe Ala Ile Thr Ala Ala Leu Ile His Ile Thr Ser Ile Pro Leu
105 110 115
ggc atc ggc atc gcc cac ctc atc gaa gaa gcc gat ctc aac cgc tgg 499
Gly Ile Gly Ile Ala His Leu Ile Glu Glu Ala Asp Leu Asn Arg Trp
120 125 130
ggc aac aac atg ttg gcc gat gtg ctg ctc acc cca gat ttc tgg gtc 547
Gly Asn Asn Met Leu Ala Asp Val Leu Leu Thr Pro Asp Phe Trp Val
135 140 145
ttc ggc gtc gcc gct ttc gca tcc gcc tcc atg cca ctg ctc tgg cga 595
Phe Gly Val Ala Ala Phe Ala Ser Ala Ser Met Pro Leu Leu Trp Arg
150 155 160 165
cgg cgc acc cga ttg ttc ctc ttt act atc acc ttg acg ctg ctg ctt 643
Arg Arg Thr Arg Leu Phe Leu Phe Thr Ile Thr Leu Thr Leu Leu Leu
170 175 180
tat acg ggc acg ctt gcc gac gtc acc atg ctc acc gcg acc atc atc 691
Tyr Thr Gly Thr Leu Ala Asp Val Thr Met Leu Thr Ala Thr Ile Ile
185 190 195
ggc acc gtt gcc ggc gag ttg aac agg cat cgg aaa acc cca ggt ggc 739
Gly Thr Val Ala Gly Glu Leu Asn Arg His Arg Lys Thr Pro Gly Gly
200 205 210
cgc tgg ctt ccc ggt tcc ctc acc gtg cgt gaa gcg cgc att atg acg 787
Arg Trp Leu Pro Gly Ser Leu Thr Val Arg Glu Ala Arg Ile Met Thr
215 220 225
gcc att ttg gtc act gcc gta gca gca ggt cca gtg ctt gct gcg ctt 835
Ala Ile Leu Val Thr Ala Val Ala Ala Gly Pro Val Leu Ala Ala Leu
230 235 240 245
aat cca ctc acc cac ggc cct ttt tcc agt gca acg aaa ttg atc tgg 883
Asn Pro Leu Thr His Gly Pro Phe Ser Ser Ala Thr Lys Leu Ile Trp
250 255 260
cag ccc ctt gtc act gaa gaa cac atg cat cac ctc tgc cac aca gac 931
Gln Pro Leu Val Thr Glu Glu His Met His His Leu Cys His Thr Asp
265 270 275
agc acc tct gat gca tgc caa ggt gcg ctt gat cag ctc caa cag cac 979
Ser Thr Ser Asp Ala Cys Gln Gly Ala Leu Asp Gln Leu Gln Gln His
280 285 290
ggt gtt ggc cct tcc gtt gcc aac ctg att cca ctg atc ctc acc gtg 1027
Gly Val Gly Pro Ser Val Ala Asn Leu Ile Pro Leu Ile Leu Thr Val
295 300 305
gtc ctt gcg atg ggg ctt agc cgc gga cgt cga ctt gca tgg att ttg 1075
Val Leu Ala Met Gly Leu Ser Arg Gly Arg Arg Leu Ala Trp Ile Leu
310 315 320 325
gcg gtt ttg gcc cag ctc att tcc atc gca gtg ttg atg ttc cag ctg 1123
Ala Val Leu Ala Gln Leu Ile Ser Ile Ala Val Leu Met Phe Gln Leu
330 335 340
acc aaa cta tcg gct gat tcc acc gat ctt cta tgg tca gtc aat gcc 1171
Thr Lys Leu Ser Ala Asp Ser Thr Asp Leu Leu Trp Ser Val Asn Ala
345 350 355
ttt agc gtg atc gtt cct tgg ctg gtg gcg ctc gcc gtc ttg gtg ttt 1219
Phe Ser Val Ile Val Pro Trp Leu Val Ala Leu Ala Val Leu Val Phe
360 365 370
tcc cgc cgt gca ttc cag gtg aag att gat acc acc cgg att tct aaa 1267
Ser Arg Arg Ala Phe Gln Val Lys Ile Asp Thr Thr Arg Ile Ser Lys
375 380 385
tcc tta ggc gct ctc atg gtt aca tgg ttg gca acg gca gca ttg tgg 1315
Ser Leu Gly Ala Leu Met Val Thr Trp Leu Ala Thr Ala Ala Leu Trp
390 395 400 405
atc ctt gcc aca ttg ttc ctg cca cac gca ttc cac cca cat cca aca 1363
Ile Leu Ala Thr Leu Phe Leu Pro His Ala Phe His Pro His Pro Thr
410 415 420
ttg ggg ctg gct ttc aaa gaa ctc ccc ttc cgc tat ctc cca cca act 1411
Leu Gly Leu Ala Phe Lys Glu Leu Pro Phe Arg Tyr Leu Pro Pro Thr
425 430 435
atc gaa acg gtg tta agc cat caa ctc ttc ccc aga agc cct gct ggg 1459
Ile Glu Thr Val Leu Ser His Gln Leu Phe Pro Arg Ser Pro Ala Gly
440 445 450
tgg gca gtg ttt gaa tgg act gga acg cta ttc tgg ctg gtc gta gca 1507
Trp Ala Val Phe Glu Trp Thr Gly Thr Leu Phe Trp Leu Val Val Ala
455 460 465
gca act ctt tat cat ctg ctc atg ggt gtg ccc agc aac aaa gcg cac 1555
Ala Thr Leu Tyr His Leu Leu Met Gly Val Pro Ser Asn Lys Ala His
470 475 480 485
gag gac caa gaa aac gca gcg act ctc ctg cgc tct ggc agc ggc gat 1603
Glu Asp Gln Glu Asn Ala Ala Thr Leu Leu Arg Ser Gly Ser Gly Asp
490 495 500
cac ttg tcc tgg atg acc att tgg ggt ggc aat acg tat tgg tgg gca 1651
His Leu Ser Trp Met Thr Ile Trp Gly Gly Asn Thr Tyr Trp Trp Ala
505 510 515
cca gaa aat gca gga tat gtg gcc tac cgc gtg aaa agg ggc atc gca 1699
Pro Glu Asn Ala Gly Tyr Val Ala Tyr Arg Val Lys Arg Gly Ile Ala
520 525 530
att aca ttg ggt gag cct att ctg ggt ccg gat tca tcc gtc tct aaa 1747
Ile Thr Leu Gly Glu Pro Ile Leu Gly Pro Asp Ser Ser Val Ser Lys
535 540 545
gca gag ctg gcc gca cag ttt gaa gaa ttt gcc agc aac caa ggc tgg 1795
Ala Glu Leu Ala Ala Gln Phe Glu Glu Phe Ala Ser Asn Gln Gly Trp
550 555 560 565
att gtt gcg tgg tat tcc gtt tgt gaa gaa ttc tca aag gaa cgc atc 1843
Ile Val Ala Trp Tyr Ser Val Cys Glu Glu Phe Ser Lys Glu Arg Ile
570 575 580
aac gct ggc cac cac aca ctt cgt gtg gct gag gaa gca gtc tta agc 1891
Asn Ala Gly His His Thr Leu Arg Val Ala Glu Glu Ala Val Leu Ser
585 590 595
tca gct aat gcg gat ttc aaa ggt aag cac ttc caa aat gtc cgc acc 1939
Ser Ala Asn Ala Asp Phe Lys Gly Lys His Phe Gln Asn Val Arg Thr
600 605 610
gcc cga aac cgc gcg gcc aaa gag ggc gta agt tcc atc tgg aca acc 1987
Ala Arg Asn Arg Ala Ala Lys Glu Gly Val Ser Ser Ile Trp Thr Thr
615 620 625
tgg gct gat ttg agt gcc gaa atg cag cac aag atc atc acg ctg tcg 2035
Trp Ala Asp Leu Ser Ala Glu Met Gln His Lys Ile Ile Thr Leu Ser
630 635 640 645
gaa gaa tgg gtc tct gat aaa gct ctg ccg gag atg ggc ttc acg ctt 2083
Glu Glu Trp Val Ser Asp Lys Ala Leu Pro Glu Met Gly Phe Thr Leu
650 655 660
ggc act gtc aac gag ctc tca gat cca gat acc tat ctt ctt ctc gcg 2131
Gly Thr Val Asn Glu Leu Ser Asp Pro Asp Thr Tyr Leu Leu Leu Ala
665 670 675
att gat gag gaa gag cat ctg cac ggt gtg acc agt tgg ttg ccg gtc 2179
Ile Asp Glu Glu Glu His Leu His Gly Val Thr Ser Trp Leu Pro Val
680 685 690
tat gaa aaa gga cgc atc gtc ggc tac aca ctt gat gtg atg cgc cgt 2227
Tyr Glu Lys Gly Arg Ile Val Gly Tyr Thr Leu Asp Val Met Arg Arg
695 700 705
gat ccg caa ggc ttt aaa tct gtc atc gag ttt ctc att tcc gag gcc 2275
Asp Pro Gln Gly Phe Lys Ser Val Ile Glu Phe Leu Ile Ser Glu Ala
710 715 720 725
gtc gtt atc gca agg gat cac gat ctg gaa tgg atg tcg atg tcc ggc 2323
Val Val Ile Ala Arg Asp His Asp Leu Glu Trp Met Ser Met Ser Gly
730 735 740
gct cct ttg agt acg ccc cca ggt gtg gcc gac gac ggc acc atc gga 2371
Ala Pro Leu Ser Thr Pro Pro Gly Val Ala Asp Asp Gly Thr Ile Gly
745 750 755
caa att ttg gag ctt ttg ggc cga gca atg gag ccg ttc tac ggt ttc 2419
Gln Ile Leu Glu Leu Leu Gly Arg Ala Met Glu Pro Phe Tyr Gly Phe
760 765 770
cgt tcc ctc gct gcg tcc aag aac aaa ttc cac cca gaa cac cac ggt 2467
Arg Ser Leu Ala Ala Ser Lys Asn Lys Phe His Pro Glu His His Gly
775 780 785
tgg tac ttg tgt tac cgc gat gaa tta tcg tta cca agc att ggc ctt 2515
Trp Tyr Leu Cys Tyr Arg Asp Glu Leu Ser Leu Pro Ser Ile Gly Leu
790 795 800 805
gcc gtt gct gcc tgc tac ctc aac gag ttt ccg ctg ccg aat tgg ctg 2563
Ala Val Ala Ala Cys Tyr Leu Asn Glu Phe Pro Leu Pro Asn Trp Leu
810 815 820
aaa aag acc gcc act tcc gcc cca agc cac agc tagaaaaacg cgtaaccttg 2616
Lys Lys Thr Ala Thr Ser Ala Pro Ser His Ser
825 830
gtgagaaacc 2626
<210>86
<211>832
<212>PRT
<213>谷氨酸棒状杆菌
<400>86
Met Lys Asp Ala Ser Gln Ser Tyr Leu Leu Phe Gly Leu Arg Trp Leu
1 5 10 15
Gly Arg Ser Leu Arg Phe Ala Pro Leu Ser Leu Leu Met Ile Val Val
20 25 30
Met Arg Gly Leu Arg Glu Val Phe Gly Ala Glu Asp Pro Ala Asn Ser
35 40 45
Ser Leu Val Asp Asn Leu Gly Leu Thr Leu Pro Trp Ser Leu Asn Asp
50 55 60
Pro His Phe Leu Thr Ala Gly Phe Ser Ala Ser Thr Thr Thr Ala Ala
65 70 75 80
Leu Met Ser Thr Leu Trp Ile Ile Val Phe Ala Val Pro Ser Glu Arg
85 90 95
Ile Leu Gly Ser Leu Lys Phe Ala Ile Thr Ala Ala Leu Ile His Ile
100 105 110
Thr Ser Ile Pro Leu Gly Ile Gly Ile Ala His Leu Ile Glu Glu Ala
115 120 125
Asp Leu Asn Arg Trp Gly Asn Asn Met Leu Ala Asp Val Leu Leu Thr
130 135 140
Pro Asp Phe Trp Val Phe Gly Val Ala Ala Phe Ala Ser Ala Ser Met
145 150 155 160
Pro Leu Leu Trp Arg Arg Arg Thr Arg Leu Phe Leu Phe Thr Ile Thr
165 170 175
Leu Thr Leu Leu Leu Tyr Thr Gly Thr Leu Ala Asp Val Thr Met Leu
180 185 190
Thr Ala Thr Ile Ile Gly Thr Val Ala Gly Glu Leu Asn Arg His Arg
195 200 205
Lys Thr Pro Gly Gly Arg Trp Leu Pro Gly Ser Leu Thr Val Arg Glu
210 215 220
Ala Arg Ile Met Thr Ala Ile Leu Val Thr Ala Val Ala Ala Gly Pro
225 230 235 240
Val Leu Ala Ala Leu Asn Pro Leu Thr His Gly Pro Phe Ser Ser Ala
245 250 255
Thr Lys Leu Ile Trp Gln Pro Leu Val Thr Glu Glu His Met His His
260 265 270
Leu Cys His Thr Asp Ser Thr Ser Asp Ala Cys Gln Gly Ala Leu Asp
275 280 285
Gln Leu Gln Gln His Gly Val Gly Pro Ser Val Ala Asn Leu Ile Pro
290 295 300
Leu Ile Leu Thr Val Val Leu Ala Met Gly Leu Ser Arg Gly Arg Arg
305 310 315 320
Leu Ala Trp Ile Leu Ala Val Leu Ala Gln Leu Ile Ser Ile Ala Val
325 330 335
Leu Met Phe Gln Leu Thr Lys Leu Ser Ala Asp Ser Thr Asp Leu Leu
340 345 350
Trp Ser Val Asn Ala Phe Ser Val Ile Val Pro Trp Leu Val Ala Leu
355 360 365
Ala Val Leu Val Phe Ser Arg Arg Ala Phe Gln Val Lys Ile Asp Thr
370 375 380
Thr Arg Ile Ser Lys Ser Leu Gly Ala Leu Met Val Thr Trp Leu Ala
385 390 395 400
Thr Ala Ala Leu Trp Ile Leu Ala Thr Leu Phe Leu Pro His Ala Phe
405 410 415
His Pro His Pro Thr Leu Gly Leu Ala Phe Lys Glu Leu Pro Phe Arg
420 425 430
Tyr Leu Pro Pro Thr Ile Glu Thr Val Leu Ser His Gln Leu Phe Pro
435 440 445
Arg Ser Pro Ala Gly Trp Ala Val Phe Glu Trp Thr Gly Thr Leu Phe
450 455 460
Trp Leu Val Val Ala Ala Thr Leu Tyr His Leu Leu Met Gly Val Prc
465 470 475 480
Ser Asn Lys Ala His Glu Asp Gln Glu Asn Ala Ala Thr Leu Leu Arg
485 490 495
Ser Gly Ser Gly Asp His Leu Ser Trp Met Thr Ile Trp Gly Gly Asn
500 505 510
Thr Tyr Trp Trp Ala Pro Glu Asn Ala Gly Tyr Val Ala Tyr Arg Val
515 520 525
Lys Arg Gly Ile Ala Ile Thr Leu Gly Glu Pro Ile Leu Gly Pro Asp
530 535 540
Ser Ser Val Ser Lys Ala Glu Leu Ala Ala Gln Phe Glu Glu Phe Ala
545 550 555 560
Ser Asn Gln Gly Trp Ile Val Ala Trp Tyr Ser Val Cys Glu Glu Phe
565 570 575
Ser Lys Glu Arg Ile Asn Ala Gly His His Thr Leu Arg Val Ala Glu
580 585 590
Glu Ala Val Leu Ser Ser Ala Asn Ala Asp Phe Lys Gly Lys His Phe
595 600 605
Gln Asn Val Arg Thr Ala Arg Asn Arg Ala Ala Lys Glu Gly Val Ser
610 615 620
Ser Ile Trp Thr Thr Trp Ala Asp Leu Ser Ala Glu Met Gln His Lys
625 630 635 640
Ile Ile Thr Leu Ser Glu Glu Trp Val Ser Asp Lys Ala Leu Pro Glu
645 650 655
Met Gly Phe Thr Leu Gly Thr Val Asn Glu Leu Ser Asp Pro Asp Thr
660 665 670
Tyr Leu Leu Leu Ala Ile Asp Glu Glu Glu His Leu His Gly Val Thr
675 680 685
Ser Trp Leu Pro Val Tyr Glu Lys Gly Arg Ile Val Gly Tyr Thr Leu
690 695 700
Asp Val Met Arg Arg Asp Pro Gln Gly Phe Lys Ser Val Ile Glu Phe
705 710 715 720
Leu Ile Ser Glu Ala Val Val Ile Ala Arg Asp His Asp Leu Glu Trp
725 730 735
Met Ser Met Ser Gly Ala Pro Leu Ser Thr Pro Pro Gly Val Ala Asp
740 745 750
Asp Gly Thr Ile Gly Gln Ile Leu Glu Leu Leu Gly Arg Ala Met Glu
755 760 765
Pro Phe Tyr Gly Phe Arg Ser Leu Ala Ala Ser Lys Asn Lys Phe His
770 775 780
Pro Glu His His Gly Trp Tyr Leu Cys Tyr Arg Asp Glu Leu Ser Leu
785 790 795 800
Pro Ser Ile Gly Leu Ala Val Ala Ala Cys Tyr Leu Asn Glu Phe Pro
805 810 815
Leu Pro Asn Trp Leu Lys Lys Thr Ala Thr Ser Ala Pro Ser His Ser
820 825 830
<210>87
<211>709
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(679)
<223>RXA00740
<400>87
gttataaata ttaaagtacg ctaaccatgg gtgcaggggg agacttctac agcagtcatg 60
gtccacacca cgactgctgt atatattaag gacgtaactc atg ttg aag aat aaa 115
Met Leu Lys Asn Lys
1 5
act ctt gcg ctg ctg gct gtc agt acg ctg atg ttt ggc tta tct gct 163
Thr Leu Ala Leu Leu Ala Val Ser Thr Leu Met Phe Gly Leu Ser Ala
10 15 20
tgc tct agt gat gct gac ggc ccg gac agt agc ggt gct agt agt agc 211
Cys Ser Ser Asp Ala Asp Gly Pro Asp Ser Ser Gly Ala Ser Ser Ser
25 30 35
agt act ggc tca tct tct gca tcg gct gac aca aca gat tct gca gct 259
Ser Thr Gly Ser Ser Ser Ala Ser Ala Asp Thr Thr Asp Ser Ala Ala
40 45 50
gtt gat aag gat gct caa tta gac acc ttt atg cgt tct gct gtg gct 307
Val Asp Lys Asp Ala Gln Leu Asp Thr Phe Met Arg Ser Ala Val Ala
55 60 65
gat gga tca tct gct gct gtg gct gtg gac aac gct gta ggt aag ggc 355
Asp Gly Ser Ser Ala Ala Val Ala Val Asp Asn Ala Val Gly Lys Gly
70 75 80 85
ttt gat aaa gct gat gcg cag gcg gct gcg gag cgc ttg ggt gat ggt 403
Phe Asp Lys Ala Asp Ala Gln Ala Ala Ala Glu Arg Leu Gly Asp Gly
90 95 100
ttc caa ttc gct gcg gat tac cag gct gtt ggc tat ttg gaa tct tgg 451
Phe Gln Phe Ala Ala Asp Tyr Gln Ala Val Gly Tyr Leu Glu Ser Trp
105 110 115
tac ttt ggt ggc gcg cga agc gag aag ctt gac gag gtg cgt agt gat 499
Tyr Phe Gly Gly Ala Arg Ser Glu Lys Leu Asp Glu Val Arg Ser Asp
120 125 130
gtc atc gct cat ttg tct gaa ttg gga ttt aca gat tta gag gct gaa 547
Val Ile Ala His Leu Ser Glu Leu Gly Phe Thr Asp Leu Glu Ala Glu
135 140 145
tcc tcg gca tct cgt att aca tta ggt gat tac tgt gat ggg gtc cct 595
Ser Ser Ala Ser Arg Ile Thr Leu Gly Asp Tyr Cys Asp Gly Val Pro
150 155 160 165
gag tat tgt gag ttg ttt ttt gat ggc gaa tct cct gac ttg tat gac 643
Glu Tyr Cys Glu Leu Phe Phe Asp Gly Glu Ser Pro Asp Leu Tyr Asp
170 175 180
atg ggt acg gag ctt gac tcg tat aag ccc act gaa taaagctatt 689
Met Gly Thr Glu Leu Asp Ser Tyr Lys Pro Thr Glu
185 190
tcacttaaag caccccgctt 709
<210>88
<211>193
<212>PRT
<213>谷氨酸棒状杆菌
<400>88
Met Leu Lys Asn Lys Thr Leu Ala Leu Leu Ala Val Ser Thr Leu Met
1 5 10 15
Phe Gly Leu Ser Ala Cys Ser Ser Asp Ala Asp Gly Pro Asp Ser Ser
20 25 30
Gly Ala Ser Ser Ser Ser Thr Gly Ser Ser Ser Ala Ser Ala Asp Thr
35 40 45
Thr Asp Ser Ala Ala Val Asp Lys Asp Ala Gln Leu Asp Thr Phe Met
50 55 60
Arg Ser Ala Val Ala Asp Gly Ser Ser Ala Ala Val Ala Val Asp Asn
65 70 75 80
Ala Val Gly Lys Gly Phe Asp Lys Ala Asp Ala Gln Ala Ala Ala Glu
85 90 95
Arg Leu Gly Asp Gly Phe Gln Phe Ala Ala Asp Tyr Gln Ala Val Gly
100 105 110
Tyr Leu Glu Ser Trp Tyr Phe Gly Gly Ala Arg Ser Glu Lys Leu Asp
115 120 125
Glu Val Arg Ser Asp Val Ile Ala His Leu Ser Glu Leu Gly Phe Thr
130 135 140
Asp Leu Glu Ala Glu Ser Ser Ala Ser Arg Ile Thr Leu Gly Asp Tyr
145 150 155 160
Cys Asp Gly Val Pro Glu Tyr Cys Glu Leu Phe Phe Asp Gly Glu Ser
165 170 175
Pro Asp Leu Tyr Asp Met Gly Thr Glu Leu Asp Ser Tyr Lys Pro Thr
180 185 190
Glu
<210>89
<211>1225
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1195)
<223>RXA00 812
<400>89
gattttctgt gcctctgcat tcgaaagtga aaattattca caggtgagcg tatcgcgggc 60
ggttgagagg tgagttatcc acaggccgca gagggggtcg ttg aaa gca cgt aag 115
Leu Lys Ala Arg Lys
1 5
cct cag gcc aca gta ggt gcc atg aac acc atc aca cac caa gca att 163
Pro Gln Ala Thr Val Gly Ala Met Asn Thr Ile Thr His Gln Ala Ile
10 15 20
ctc atc gca gtg gaa gat ccg gtc ctg cac cca gag gcc atg cac gta 211
Leu Ile Ala Val Glu Asp Pro Val Leu His Pro Glu Ala Met His Val
25 30 35
gct gca gcc act ggt cgg ccg gtt att gaa aca aca aat ttg atg gac 259
Ala Ala Ala Thr Gly Arg Pro Val Ile Glu Thr Thr Asn Leu Met Asp
40 45 50
atc tcc agg cat ttt cac cgc aca tcg gca gtg ctc att gat gcg tcg 307
Ile Ser Arg His Phe His Arg Thr Ser Ala Val Leu Ile Asp Ala Ser
55 60 65
atg gcc tct caa tta tca cct ggg aaa cgc cgc gac agg gtg ttt ctt 355
Met Ala Ser Gln Leu Ser Pro Gly Lys Arg Arg Asp Arg Val Phe Leu
70 75 80 85
ctc gat tct gat cca ggg ccc tct gat tgg aaa acg gcg atg aaa atc 403
Leu Asp Ser Asp Pro Gly Pro Ser Asp Trp Lys Thr Ala Met Lys Ile
90 95 100
cac gct gag caa gcc atg ttg ctt ccc gct cag gca ggg gag ttg ctc 451
His Ala Glu Gln Ala Met Leu Leu Pro Ala Gln Ala Gly Glu Leu Leu
105 110 115
agc gct tta ggc aga gat gac aaa caa ctg ccg gtg gct tcg ggc cat 499
Ser Ala Leu Gly Arg Asp Asp Lys Gln Leu Pro Val Ala Ser Gly His
120 125 130
gtc atc ggt gtt gcc ggg gtg gtg gga gga aca ggc gcg agc acg ttt 547
Val Ile Gly Val Ala Gly Val Val Gly Gly Thr Gly Ala Ser Thr Phe
135 140 145
gct gcg gcg ctc gca aag cgg cgt gcg gag tca gtc acc act gtg ctt 595
Ala Ala Ala Leu Ala Lys Arg Arg Ala Glu Ser Val Thr Thr Val Leu
150 155 160 165
atc gac gcc gac cct tcc tct ggc ggc atc gac ctg ttg cta gga atc 643
Ile Asp Ala Asp Pro Ser Ser Gly Gly Ile Asp Leu Leu Leu Gly Ile
170 175 180
gaa gat gtg ccc ggc gcg cgg tgg cct gat gtg ggg ttg cgt cgc gga 691
Glu Asp Val Pro Gly Ala Arg Trp Pro Asp Val Gly Leu Arg Arg Gly
185 190 195
act gtc cag gct gct gat gtg ttg aaa gcg ctg ccg agc act cct gat 739
Thr Val Gln Ala Ala Asp Val Leu Lys Ala Leu Pro Ser Thr Pro Asp
200 205 210
gag gtc gtg gtg ttg tct acg gcg cgg tct aat att ctg gat cct ttt 787
Glu Val Val Val Leu Ser Thr Ala Arg Ser Asn Ile Leu Asp Pro Phe
215 220 225
gca tta tcc gag tct gat gtt tcg gcg gcg att gat tgt ttc tta agc 835
Ala Leu Ser Glu Ser Asp Val Ser Ala Ala Ile Asp Cys Phe Leu Ser
230 235 240 245
gcc gat agg tcg gtg gat gtg gtg gtg gat ctg cct cat gcg aga gtg 883
Ala Asp Arg Ser Val Asp Val Val Val Asp Leu Pro His Ala Arg Val
250 255 260
cat cca gat att gcg gag cgc tta tcc cac ctg gtg tta gtc atc cct 931
His Pro Asp Ile Ala Glu Arg Leu Ser His Leu Val Leu Val Ile Pro
265 270 275
gca gag gtc cga gcg gtg gct gct gcc agg gct cgg tgt ctg gaa tta 979
Ala Glu Val Arg Ala Val Ala Ala Ala Arg Ala Arg Cys Leu Glu Leu
280 285 290
caa caa ttg cat gta tcc atc acg tgc gtg ttg agg cat cgc ggg tgg 1027
Gln Gln Leu His Val Ser Ile Thr Cys Val Leu Arg His Arg Gly Trp
295 300 305
tca ggt ttg gat gtt gca gaa gtg gaa gag att tta ggt gcg gat att 1075
Ser Gly Leu Asp Val Ala Glu Val Glu Glu Ile Leu Gly Ala Asp Ile
310 315 320 325
acc gcg gag gtt ggc tcg atc cag cgg ctg gct aag tcc gtg gag atg 1123
Thr Ala Glu Val Gly Ser Ile Gln Arg Leu Ala Lys Ser Val Glu Met
330 335 340
cat ggg ctg acc ggt tct ttg ccg agg gtt tta agc tca gca tgc gac 1171
His Gly Leu Thr Gly Ser Leu Pro Arg Val Leu Ser Ser Ala Cys Asp
345 350 355
gcg gtc ctc ggg gag gtg gcg gca tgactgacat tgatctggtg gtggaaaacg 1225
Ala Val Leu Gly Glu Val Ala Ala
360 365
<210>90
<211>365
<212>PRT
<213>谷氨酸棒状杆菌
<400>90
Leu Lys Ala Arg Lys Pro Gln Ala Thr Val Gly Ala Met Asn Thr Ile
1 5 10 15
Thr His Gln Ala Ile Leu Ile Ala Val Glu Asp Pro Val Leu His Pro
20 25 30
Glu Ala Met His Val Ala Ala Ala Thr Gly Arg Pro Val Ile Glu Thr
35 40 45
Thr Asn Leu Met Asp Ile Ser Arg His Phe His Arg Thr Ser Ala Val
50 55 60
Leu Ile Asp Ala Ser Met Ala Ser Gln Leu Ser Pro Gly Lys Arg Arg
65 70 75 80
Asp Arg Val Phe Leu Leu Asp Ser Asp Pro Gly Pro Ser Asp Trp Lys
85 90 95
Thr Ala Met Lys Ile His Ala Glu Gln Ala Met Leu Leu Pro Ala Gln
100 105 110
Ala Gly Glu Leu Leu Ser Ala Leu Gly Arg Asp Asp Lys Gln Leu Pro
115 120 125
Val Ala Ser Gly His Val Ile Gly Val Ala Gly Val Val Gly Gly Thr
130 135 140
Gly Ala Ser Thr Phe Ala Ala Ala Leu Ala Lys Arg Arg Ala Glu Ser
145 150 155 160
Val Thr Thr Val Leu Ile Asp Ala Asp Pro Ser Ser Gly Gly Ile Asp
165 170 175
Leu Leu Leu Gly Ile Glu Asp Val Pro Gly Ala Arg Trp Pro Asp Val
180 185 190
Gly Leu Arg Arg Gly Thr Val Gln Ala Ala Asp Val Leu Lys Ala Leu
195 200 205
Pro Ser Thr Pro Asp Glu Val Val Val Leu Ser Thr Ala Arg Ser Asn
210 215 220
Ile Leu Asp Pro Phe Ala Leu Ser Glu Ser Asp Val Ser Ala Ala Ile
225 230 235 240
Asp Cys Phe Leu Ser Ala Asp Arg Ser Val Asp Val Val Val Asp Leu
245 250 255
Pro His Ala Arg Val His Pro Asp Ile Ala Glu Arg Leu Ser His Leu
260 265 270
Val Leu Val Ile Pro Ala Glu Val Arg Ala Val Ala Ala Ala Arg Ala
275 280 285
Arg Cys Leu Glu Leu Gln Gln Leu His Val Ser Ile Thr Cys Val Leu
290 295 300
Arg His Arg Gly Trp Ser Gly Leu Asp Val Ala Glu Val Glu Glu Ile
305 310 315 320
Leu Gly Ala Asp Ile Thr Ala Glu Val Gly Ser Ile Gln Arg Leu Ala
325 330 335
Lys Ser Val Glu Met His Gly Leu Thr Gly Ser Leu Pro Arg Val Leu
340 345 350
Ser Ser Ala Cys Asp Ala Val Leu Gly Glu Val Ala Ala
355 360 365
<210>91
<211>832
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(802)
<223>RXA00831
<400>91
agctccaaga aagtcagtgc tgtgcatggc tcggtgctgc tcatgctttt cggtgtttac 60
atgatgagca tgttcgcctg atttaggtag cctggtggga atg agt gca ctt gag 115
Met Ser Ala Leu Glu
1 5
aca ttg caa tgg cag gac tgg tca agc gtc tta att gtg gta gct cac 163
Thr Leu Gln Trp Gln Asp Trp Ser Ser Val Leu Ile Val Val Ala His
10 15 20
cca gat gat ccg gag tat ggg ctt tcc gcg gct gtt aaa gaa tgg aca 211
Pro Asp Asp Pro Glu Tyr Gly Leu Ser Ala Ala Val Lys Glu Trp Thr
25 30 35
gac gcc ggg gtg gag gtg tct tac ctg ctg ctc acc cac ggg gag gca 259
Asp Ala Gly Val Glu Val Ser Tyr Leu Leu Leu Thr His Gly Glu Ala
40 45 50
ggt atc caa ggt tta gac cct aaa gaa acc ggg tca ttg cgc gca gcg 307
Gly Ile Gln Gly Leu Asp Pro Lys Glu Thr Gly Ser Leu Arg Ala Ala
55 60 65
gaa cag cgg gct gca tgt gat gtg gta gga gtt aga aat ctc acc att 355
Glu Gln Arg Ala Ala Cys Asp Val Val Gly Val Arg Asn Leu Thr Ile
70 75 80 85
ttg aat cac cca gat tcc atg ttg gtg tac aat ctg gta ctg cgc aaa 403
Leu Asn His Pro Asp Ser Met Leu Val Tyr Asn Leu Val Leu Arg Lys
90 95 100
gat att gct cgg gaa atc cgg atc cgt aaa cca aat gct gtg gtg gta 451
Asp Ile Ala Arg Glu Ile Arg Ile Arg Lys Pro Asn Ala Val Val Val
105 110 115
tcc aat ttt gat gta gag gcc tac ggt ggt ttg aac cag gcg gat cac 499
Ser Asn Phe Asp Val Glu Ala Tyr Gly Gly Leu Asn Gln Ala Asp His
120 125 130
cgc gtg gcg gga tta gcc gca att gat gcg acc cgc gat gcc gct aat 547
Arg Val Ala Gly Leu Ala Ala Ile Asp Ala Thr Arg Asp Ala Ala Asn
135 140 145
ccg tgg gcg cag cca gag ctg ttg cag gag gat ctg cag ccg tgg gga 595
Pro Trp Ala Gln Pro Glu Leu Leu Gln Glu Asp Leu Gln Pro Trp Gly
150 155 160 165
gct gaa gtc atc atc att gcc gga cac cca gag ccc acc cac acc atg 643
Ala Glu Val Ile Ile Ile Ala Gly His Pro Glu Pro Thr His Thr Met
170 175 180
gat ctg gct aaa gat tct gtt gat gcc gga gtt gca tcc ctt caa gct 691
Asp Leu Ala Lys Asp Ser Val Asp Ala Gly Val Ala Ser Leu Gln Ala
185 190 195
cac aag gaa tac ttg gcc gct ctt cca gat ccc ccg aag ccg gag gag 739
His Lys Glu Tyr Leu Ala Ala Leu Pro Asp Pro Pro Lys Pro Glu Glu
200 205 210
ttc att ccg gcg ttt ctc gag gta gag ggc ggt tac gca gcg gcc ttc 787
Phe Ile Pro Ala Phe Leu Glu Val Glu Gly Gly Tyr Ala Ala Ala Phe
215 220 225
cga gtt ttc gga cgg taagcagggc gatacgtgat aggcagctta 832
Arg Val Phe Gly Arg
230
<210>92
<211>234
<212>PRT
<213>谷氨酸棒状杆菌
<400>92
Met Ser Ala Leu Glu Thr Leu Gln Trp Gln Asp Trp Ser Ser Val Leu
1 5 10 15
Ile Val Val Ala His Pro Asp Asp Pro Glu Tyr Gly Leu Ser Ala Ala
20 25 30
Val Lys Glu Trp Thr Asp Ala Gly Val Glu Val Ser Tyr Leu Leu Leu
35 40 45
Thr His Gly Glu Ala Gly Ile Gln Gly Leu Asp Pro Lys Glu Thr Gly
50 55 60
Ser Leu Arg Ala Ala Glu Gln Arg Ala Ala Cys Asp Val Val Gly Val
65 70 75 80
Arg Asn Leu Thr Ile Lau Asn His Pro Asp Ser Met Leu Val Tyr Asn
85 90 95
Leu Val Leu Arg Lys Asp Ile Ala Arg Glu Ile Arg Ile Arg Lys Pro
100 105 110
Asn Ala Val Val Val Ser Asn Phe Asp Val Glu Ala Tyr Gly Gly Leu
115 120 125
Asn Gln Ala Asp His Arg Val Ala Gly Leu Ala Ala Ile Asp Ala Thr
130 135 140
Arg Asp Ala Ala Asn Pro Trp Ala Gln Pro Glu Leu Leu Gln Glu Asp
145 150 155 160
Leu Gln Pro Trp Gly Ala Glu Val Ile Ile Ile Ala Gly His Pro Glu
165 170 175
Pro Thr His Thr Met Asp Leu Ala Lys Asp Ser Val Asp Ala Gly Val
180 185 190
Ala Ser Leu Gln Ala His Lys Glu Tyr Leu Ala Ala Leu Pro Asp Pro
195 200 205
Pro Lys Pro Glu Glu Phe Ile Pro Ala Phe Leu Glu Val Glu Gly Gly
210 215 220
Tyr Ala Ala Ala Phe Arg Val Phe Gly Arg
225 230
<210>93
<211>1072
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1042)
<223>RXA00835
<400>93
ccaacgatcg caattttcat acctgctatc ttgcctgagc acagataaga tttgtaagca 60
ctaccccttc tctacaaagg agtcatcaat ggccggtgga ttg gca gca cta tta 115
Leu Ala Ala Leu Leu
1 5
gac gat gtc gca gca att act cga gcg gct gca gcc agc ctc gat gat 163
Asp Asp Val Ala Ala Ile Thr Arg Ala Ala Ala Ala Ser Leu Asp Asp
10 15 20
gtc acg gcg atg gct gga aaa acc agc gtt aaa gcc gca ggc gtg gtt 211
Val Thr Ala Met Ala Gly Lys Thr Ser Val Lys Ala Ala Gly Val Val
25 30 35
gta gat gac acg gca gtt acc ccg caa tat gtt caa ggt gtc aaa cct 259
Val Asp Asp Thr Ala Val Thr Pro Gln Tyr Val Gln Gly Val Lys Pro
40 45 50
gca cgc gaa ctg ccc atg att tgg cgg atc gct aag ggc tct ttg gta 307
Ala Arg Glu Leu Pro Met Ile Trp Arg Ile Ala Lys Gly Ser Leu Val
55 60 65
aac aag atc atc atc att ttg ccc atc gcg ttg ctt ctt tcc gcg ttt 355
Asn Lys Ile Ile Ile Ile Leu Pro Ile Ala Leu Leu Leu Ser Ala Phe
70 75 80 85
gct ccg tgg gct ctc aca cca att ttg atg ctg ggc ggc tcc tac ctg 403
Ala Pro Trp Ala Leu Thr Pro Ile Leu Met Leu Gly Gly Ser Tyr Leu
90 95 100
tgc ttc gaa ggt gca gaa aag atc tgg cac tcc ctc cac agg cgc atc 451
Cys Phe Glu Gly Ala Glu Lys Ile Trp His Ser Leu His Arg Arg Ile
105 110 115
aaa ggt gaa cag cac agc acc gaa ccc aag tcg cag gaa agc cca aag 499
Lys Gly Glu Gln His Ser Thr Glu Pro Lys Ser Gln Glu Ser Pro Lys
120 125 130
tca gag gat cag ctg gtc aaa agt gcc atc aca act gac ctc atc ctt 547
Ser Glu Asp Gln Leu Val Lys Ser Ala Ile Thr Thr Asp Leu Ile Leu
135 140 145
tcc gca gag atc atg gtt att tca ctc aat cag att gca gat caa act 595
Ser Ala Glu Ile Met Val Ile Ser Leu Asn Gln Ile Ala Asp Gln Thr
150 155 160 165
atc tgg atg cag gct gcg gtt ctt ttc gtc gta ggt att ggc atc acc 643
Ile Trp Met Gln Ala Ala Val Leu Phe Val Val Gly Ile Gly Ile Thr
170 175 180
gcg ctc gtg tac ggc gta gtc ggt gtt ctt gtg aaa atg gat gac gtc 691
Ala Leu Val Tyr Gly Val Val Gly Val Leu Val Lys Met Asp Asp Val
185 190 195
gga ctt aca ctt tca aag cgt gac tcc gca ggt atc cag aaa ttt ggc 739
Gly Leu Thr Leu Ser Lys Arg Asp Ser Ala Gly Ile Gln Lys Phe Gly
200 205 210
cgc ggc ttg gtc aaa gcg atg ccc atc gtt ctt caa gtt att tct gtt 787
Arg Gly Leu Val Lys Ala Met Pro Ile Val Leu Gln Val Ile Ser Val
215 220 225
gtc ggc gtt ttt gcc atg ctg tgg gtt ggt ggc cac atc atg gtc gtt 835
Val Gly Val Phe Ala Met Leu Trp Val Gly Gly His Ile Met Val Val
230 235 240 245
gga act gaa gag tta gga tgg gaa ctt ccc tac cac ctt gtt cac gga 883
Gly Thr Glu Glu Leu Gly Trp Glu Leu Pro Tyr His Leu Val His Gly
250 255 260
cta gag tcc tgg gct aac ggt atc gga ggc agt gct ctg gga tgg gtt 931
Leu Glu Ser Trp Ala Asn Gly Ile Gly Gly Ser Ala Leu Gly Trp Val
265 270 275
ggc aat act ttc ggg tca ctt gtg ttc ggc ctc att tgg ggc gcg att 979
Gly Asn Thr Phe Gly Ser Leu Val Phe Gly Leu Ile Trp Gly Ala Ile
280 285 290
atc acc gtt gtg gtc agt gtg atc aag aag ttc att cca cag cgt gcg 1027
Ile Thr Val Val Val Ser Val Ile Lys Lys Phe Ile Pro Gln Arg Ala
295 300 305
caa aac tcg tct cat tagtggagag ttgttcgctg taatttcgct 1072
Gln Asn Ser Ser His
310
<210>94
<211>314
<212>PRT
<213>谷氨酸棒状杆菌
<400>94
Leu Ala Ala Leu Leu Asp Asp Val Ala Ala Ile Thr Arg Ala Ala Ala
1 5 10 15
Ala Ser Leu Asp Asp Val Thr Ala Met Ala Gly Lys Thr Ser Val Lys
20 25 30
Ala Ala Gly Val Val Val Asp Asp Thr Ala Val Thr Pro Gln Tyr Val
35 40 45
Gln Gly Val Lys Pro Ala Arg Glu Leu Pro Met Ile Trp Arg Ile Ala
50 55 60
Lys Gly Ser Leu Val Asn Lys Ile Ile Ile Ile Leu Pro Ile Ala Leu
65 70 75 80
Leu Leu Ser Ala Phe Ala Pro Trp Ala Leu Thr Pro Ile Leu Met Leu
85 90 95
Gly Gly Ser Tyr Leu Cys Phe Glu Gly Ala Glu Lys Ile Trp His Ser
100 105 110
Leu His Arg Arg Ile Lys Gly Glu Gln His Ser Thr Glu Pro Lys Ser
115 120 125
Gln Glu Ser Pro Lys Ser Glu Asp Gln Leu Val Lys Ser Ala Ile Thr
130 135 140
Thr Asp Leu Ile Leu Ser Ala Glu Ile Met Val Ile Ser Leu Asn Gln
145 150 155 160
Ile Ala Asp Gln Thr Ile Trp Met Gln Ala Ala Val Leu Phe Val Val
165 170 175
Gly Ile Gly Ile Thr Ala Leu Val Tyr Gly Val Val Gly Val Leu Val
180 185 190
Lys Met Asp Asp Val Gly Leu Thr Leu Ser Lys Arg Asp Ser Ala Gly
195 200 205
Ile Gln Lys Phe Gly Arg Gly Leu Val Lys Ala Met Pro Ile Val Leu
210 215 220
Gln Val Ile Ser Val Val Gly Val Phe Ala Met Leu Trp Val Gly Gly
225 230 235 240
His Ile Met Val Val Gly Thr Glu Glu Leu Gly Trp Glu Leu Pro Tyr
245 250 255
His Leu Val His Gly Leu Glu Ser Trp Ala Asn Gly Ile Gly Gly Ser
260 265 270
Ala Leu Gly Trp Val Gly Asn Thr Phe Gly Ser Leu Val Phe Gly Leu
275 280 285
Ile Trp Gly Ala Ile Ile Thr Val Val Val Ser Val Ile Lys Lys Phe
290 295 300
Ile Pro Gln Arg Ala Gln Asn Ser Ser His
305 310
<210>95
<211>2809
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2779)
<223>RXA00917
<400>95
tgccgtggaa gaaagcgctc tttactgcga cagccgtcct attggtgttt gctgttgtgt 60
ggtcggcctt ggatattgcc ctgtgggtag gaaagcagtc atg gtg aac acg ttg 115
Met Val Asn Thr Leu
1 5
aac tct aaa acc gtg aat gta ccc cgt ttt gcc aga ggc gtt gtt gct 163
Asn Ser Lys Thr Val Asn Val Pro Arg Phe Ala Arg Gly Val Val Ala
10 15 20
gca gcc aca gcg cta ttt ttt ggc gct ttg gta agc ctc gcg cct agt 211
Ala Ala Thr Ala Leu Phe Phe Gly Ala Leu Val Ser Leu Ala Pro Ser
25 30 35
gcg ttg gcg cag gaa cca cct gca gtt gag gcc gga gcg tca ggt tct 259
Ala Leu Ala Gln Glu Pro Pro Ala Val Glu Ala Gly Ala Ser Gly Ser
40 45 50
ttg agc aac ctg ggt gcc tgc atc gct gat aaa ggc act ctt gat gtc 307
Leu Ser Asn Leu Gly Ala Cys Ile Ala Asp Lys Gly Thr Leu Asp Val
55 60 65
atc atc atg atc gat gag aca gaa tcc ttg atc cat gaa gct cgt gac 355
Ile Ile Met Ile Asp Glu Thr Glu Ser LeuIle His Glu Ala Arg Asp
70 75 80 85
ggc gtg gtc aac gcc aat gaa cca ggc gcg gac gca cag cac cac cgt 403
Gly Val Val Asn Ala Asn Glu Pro Gly Ala Asp Ala Gln His His Arg
90 95 100
gtt cct gca gca cag agc ttt gtg gat gag ctt cta gcc aag caa agc 451
Val Pro Ala Ala Gln Ser Phe Val Asp Glu Leu Leu Ala Lys Gln Ser
105 110 115
gat ggt gat ctg aac acc cgc atc cgt gtt gct ggt ttc ggc cag acg 499
Asp Gly Asp Leu Asn Thr Arg Ile Arg Val Ala Gly Phe Gly Gln Thr
120 125 130
tac aaa tct ggt gcc act gat cca gac aat tac gga gca tgg acg caa 547
Tyr Lys Ser Gly Ala Thr Asp Pro Asp Asn Tyr Gly Ala Trp Thr Gln
135 140 145
cta gat gcc tcc acc gtt ggc gga gta caa gat gaa atc tcc cgc ttc 595
Leu Asp Ala Ser Thr Val Gly Gly Val Gln Asp Glu Ile Ser Arg Phe
150 155 160 165
gct gac cgc acc cag gag cag tac acc aac tac gcc tcc gcg att gag 643
Ala Asp Arg Thr Gln Glu Gln Tyr Thr Asn Tyr Ala Ser Ala Ile Glu
170 175 180
ggc gct tac cag gac ttc act agg tcc ggc tct gag gac gcc tgc cgc 691
Gly Ala Tyr Gln Asp Phe Thr Arg Ser Gly Ser Glu Asp Ala Cys Arg
185 190 195
atg ctg gtg acc ttc acc gac ggc gca ctg acc gct caa gaa ggc gcc 739
Met Leu Val Thr Phe Thr Asp Gly Ala Leu Thr Ala Gln Glu Gly Ala
200 205 210
gat gtt gca gaa gca gca ctg tgc gcg ccg ggt ggc gtc acc gat cga 787
Asp Val Ala Glu Ala Ala Leu Cys Ala Pro Gly Gly Val Thr Asp Arg
215 220 225
ctg cgc agt gct ggc atc acc cac atc ggc atc ggt tta tcg gca cct 835
Leu Arg Ser Ala Gly Ile Thr His Ile Gly Ile Gly Leu Ser Ala Pro
230 235 240 245
acc aac cca tct gat ttc agc ctg ctg cgc gga acc acc gca ggt ggc 883
Thr Asn Pro Ser Asp Phe Ser Leu Leu Arg Gly Thr Thr Ala Gly Gly
250 255 260
gga aca tgt ggt gtc gaa cca gct aac ggt gca ttc ttc cca gca gat 931
Gly Thr Cys Gly Val Glu Pro Ala Asn Gly Ala Phe Phe Pro Ala Asp
265 270 275
aac gtg ggc gga ctt ttc gca gca ttc cgt gaa gcc crt gcg att ggt 979
Asn Val Gly Gly Leu Phe Ala Ala Phe Arg Glu Ala Leu Ala Ile Gly
280 285 290
ggt gaa aca att ggt gaa acc cga gct ggt gat cct ttc agc ttc acc 1027
Gly Glu Thr Ile Gly Glu Thr Arg Ala Gly Asp Pro Phe Ser Phe Thr
295 300 305
ctg gac aac tcg gtg aac tct gtg cgt ttc acc gcg atc gcc aag gat 1075
Leu Asp Asn Ser Val Asn Ser Val Arg Phe Thr Ala Ile Ala Lys Asp
310 315 320 325
gat ctc ggc ccg aac gcc cac ttg gtg ctc acc gca ccc aac ggc gaa 1123
Asp Leu Gly Pro Asn Ala His Leu Val Leu Thr Ala Pro Asn Gly Glu
330 335 340
acg gtt gag ctc aaa gat tct gga agc agc gtc gcc aac agc act gac 1171
Thr Val Glu Leu Lys Asp Ser Gly Ser Ser Val Ala Asn Ser Thr Asp
345 350 355
gtg agt tgg gaa gcc gaa agc agc cca gta aaa atg gct gat ggt tcc 1219
Val Ser Trp Glu Ala Glu Ser Ser Pro Val Lys Met Ala Asp Gly Ser
360 365 370
ctc aac ctg cag caa ggt ggg gat tgg aag ggt gtc tgg cag att cag 1267
Leu Asn Leu Gln Gln Gly Gly Asp Trp Lys Gly Val Trp Gln Ile Gln
375 380 385
ttc caa gga att gat cct gca gcg gtt gat gga cgc gta ttc aac tca 1315
Phe Gln Gly Ile Asp Pro Ala Ala Val Asp Gly Arg Val Phe Asn Ser
390 395 400 405
gtg gag atc cag cct gac ctc cag ctt gtg ttc agt ggc ggt gat tcg 1363
Val Glu Ile Gln Pro Asp Leu Gln Leu Val Phe Ser Gly Gly Asp Ser
410 415 420
acg tca ggt gca ctt aac ctt cgc gat gat cag cag ctg aat atg cag 1411
Thr Ser Gly Ala Leu Asn Leu Arg Asp Asp Gln Gln Leu Asn Met Gln
425 430 435
ctg gtg ggc cgt gat ggt cag cca cgc atc ctt gag ggg tcc gcg ctg 1459
Leu Val Gly Arg Asp Gly Gln Pro Arg Ile Leu Glu Gly Ser Ala Leu
440 445 450
gtc gat ctt ggt ttc acc cgc gca gat acc ggc gag ttc gcc cct ttg 1507
Val Asp Leu Gly Phe Thr Arg Ala Asp Thr Gly Glu Phe Ala Pro Leu
455 460 465
gct caa gga att gat att tct ggc ggc gaa tta agc ttc ccg ctg gat 1555
Ala Gln Gly Ile Asp Ile Ser Gly Gly Glu Leu Ser Phe Pro Leu Asp
470 475 480 485
acg att tcg cag ctc cca gcc atc ggc acg gtg gaa gcg cgt acc acg 1603
Thr Ile Ser Gln Leu Pro Ala Ile Gly Thr Val Glu Ala Arg Thr Thr
490 495 500
atc acc acc gca ggc gtc gat gat ctc ccc ggc acc acg ttg agc cca 1651
Ile Thr Thr Ala Gly Val Asp Asp Leu Pro Gly Thr Thr Leu Ser Pro
505 510 515
att ctc aac acc acg cgc atc acc atc act cag cgc gat atg cct cag 1699
Ile Leu Asn Thr Thr Arg Ile Thr Ile Thr Gln Arg Asp Met Pro Gln
520 525 530
ctg cca gcg tcg gtt cgc ttc aca gcg gat gag gac gtt gtt acc gta 1747
Leu Pro Ala Ser Val Arg Phe Thr Ala Asp Glu Asp Val Val Thr Val
535 540 545
gac atc ccc atc acc ggc ccc ggc aag gta tgg att gca ccg gga acc 1795
Asp Ile Pro Ile Thr Gly Pro Gly Lys Val Trp Ile Ala Pro Gly Thr
550 555 560 565
cag ctc agc gga gtg ctt cca gac ggc gtg gac ggc att gca gca tca 1843
Gln Leu Ser Gly Val Leu Pro Asp Gly Val Asp Gly Ile Ala Ala Ser
570 575 580
agt act ttc gac agc cca gat aat gct ttg gtg ctc gga ctg gat gag 1891
Ser Thr Phe Asp Ser Pro Asp Asn Ala Leu Val Leu Gly Leu Asp Glu
585 590 595
cag ggc acg att cct gtt gaa cta acc gtg agc gat ctt cgt gac gga 1939
Gln Gly Thr Ile Pro Val Glu Leu Thr Val Ser Asp Leu Arg Asp Gly
600 605 610
ctg gtc aac ggc tcg att cca ctc cag atc tcc aac gct gag ggc gcc 1987
Leu Val Asn Gly Ser Ile Pro Leu Gln Ile Ser Asn Ala Glu Gly Ala
615 620 625
aat gaa acc agc gtg gat ctg ccg aca gaa ggc acg ttg agc gtt ccc 2035
Asn Glu Thr Ser Val Asp Leu Pro Thr Glu Gly Thr Leu Ser Val Pro
630 635 640 645
att aac gcc tcc act ttc gca ttg gca ttc att ttg gcc ctt gtg ctt 2083
Ile Asn Ala Ser Thr Phe Ala Leu Ala Phe Ile Leu Ala Leu Val Leu
650 655 660
tcc ctc ctg att ccg ctg ctc att ttg tat att gtg cgt ttc ctc tcc 2131
Ser Leu Leu Ile Pro Leu Leu Ile Leu Tyr Ile Val Arg Phe Leu Ser
665 670 675
gca aag gtt ccg tcc tct gcg atg agt ggc gtg cgc att cca gtg gaa 2179
Ala Lys Val Pro Ser Ser Ala Met Ser Gly Val Arg Ile Pro Val Glu
680 685 690
ttc tcc ggt gaa gct ctg cgc tat gcg ggc agc act atg cct gat ctc 2227
Phe Ser Gly Glu Ala Leu Arg Tyr Ala Gly Ser Thr Met Pro Asp Leu
695 700 705
gca tcg caa acc acc gcc acc aag cag gtc gtt gtt cat gga gac acc 2275
Ala Ser Gln Thr Thr Ala Thr Lys Gln Val Val Val His Gly Asp Thr
710 715 720 725
ttc aat gtg gaa ggc cac aaa ctt aaa gtc cag cgc ttc cag ctg aac 2323
Phe Ash Val Glu Gly His Lys Leu Lys Val Gln Arg Phe Gln Leu Asn
730 735 740
ccg att gcg tct cct gca gtg atc gtg cag acc gac ccg tcg atc agc 2371
Pro Ile Ala Ser Pro Ala Val Ile Val Gln Thr Asp Pro Ser Ile Ser
745 750 755
ttc gac ggc aaa caa aag ggc aca caa gct aaa ctc ccg ctg gcg gtc 2419
Phe Asp Gly Lys Gln Lys Gly Thr Gln Ala Lys Leu Pro Leu Ala Val
760 765 770
caa ggc agt tgg ttc ctc act gca agc ggc gct gac cct tcc aag atg 2467
Gln Gly Ser Trp Phe Leu Thr Ala Ser Gly Ala Asp Pro Ser Lys Met
775 780 785
gaa ctc atc gcc ctg aca aac ctg ccg ctc gag cag ggc caa atc gac 2515
Glu Leu Ile Ala Leu Thr Asn Leu Pro Leu Glu Gln Gly Gln Ile Asp
790 795 800 805
cgc atg atc gca ggt atc acc agc aaa gcc cct gat agg gca cgc gaa 2563
Arg Met Ile Ala Gly Ile Thr Ser Lys Ala Pro Asp Arg Ala Arg Glu
810 815 820
cta caa aaa ttg ctt gac gac gcc gcg acc tcc cag ccc gca aag gtt 2611
Leu Gln Lys Leu Leu Asp Asp Ala Ala Thr Ser Gln Pro Ala Lys Val
825 830 835
cca ccg cgc gcc cca gcc gcg cag ggc cac gtc gaa aag caa gct cct 2659
Pro Pro Arg Ala Pro Ala Ala Gln Gly His Val Glu Lys Gln Ala Pro
840 845 850
agt ttt ggc act ggt tcc ggt ggt ggt ttc ggc tcc agc aat ggt gga 2707
Ser Phe Gly Thr Gly Ser Gly Gly Gly Phe Gly Ser Ser Asn Gly Gly
855 860 865
ggc ttt ggc tcc ggt agc gga tcc aac gac aca aat ggt gga ttt ggt 2755
Gly Phe Gly Ser Gly Ser Gly Ser Asn Asp Thr Asn Gly Gly Phe Gly
870 875 880 885
tcc agc ggc ggc ttc ggc gcg cga taacctgtaa cttgcgatta aaaactaact 2809
Ser Ser Gly Gly Phe Gly Ala Arg
890
<210>96
<211>893
<212>PRT
<213>谷氨酸棒状杆菌
<400>96
Met Val Asn Thr Leu Asn Ser Lys Thr Val Asn Val Pro Arg Phe Ala
1 5 10 15
Arg Gly Val Val Ala Ala Ala Thr Ala Leu Phe Phe Gly Ala Leu Val
20 25 30
Ser Leu Ala Pro Ser Ala Leu Ala Gln Glu Pro Pro Ala Val Glu Ala
35 40 45
Gly Ala Ser Gly Ser Leu Ser Asn Leu Gly Ala Cys Ile Ala Asp Lys
50 55 60
Gly Thr Leu Asp Val Ile Ile Met Ile Asp Glu Thr Glu Ser Leu Ile
65 70 75 80
His Glu Ala Arg Asp Gly Val Val Asn Ala Asn Glu Pro Gly Ala Asp
85 90 95
Ala Gln His His Arg Val Pro Ala Ala Gln Ser Phe Val Asp Glu Leu
100 105 110
Leu Ala Lys Gln Ser Asp Gly Asp Leu Ash Thr Arg Ile Arg Val Ala
115 120 125
Gly Phe Gly Gln Thr Tyr Lys Ser Gly Ala Thr Asp Pro Asp Asn Tyr
130 135 140
Gly Ala Trp Thr Gln Leu Asp Ala Ser Thr Val Gly Gly Val Gln Asp
145 150 155 160
Glu Ile Ser Arg Phe Ala Asp Arg Thr Gln Glu Gln Tyr Thr Asn Tyr
165 170 175
Ala Ser Ala Ile Glu Gly Ala Tyr Gln Asp Phe Thr Arg Ser Gly Ser
180 185 190
Glu Asp Ala Cys Arg Met Leu Val Thr Phe Thr Asp Gly Ala Leu Thr
195 200 205
Ala Gln Glu Gly Ala Asp Val Ala Glu Ala Ala Leu Cys Ala Pro Gly
210 215 220
Gly Val Thr Asp Arg Leu Arg Ser Ala Gly Ile Thr His Ile Gly Ile
225 230 235 240
Gly Leu Ser Ala Pro Thr Asn Pro Ser Asp Phe Ser Leu Leu Arg Gly
245 250 255
Thr Thr Ala Gly Gly Gly Thr Cys Gly Val Glu Pro Ala Asn Gly Ala
260 265 270
Phe Phe Pro Ala Asp Asn Val Gly Gly Leu Phe Ala Ala Phe Arg Glu
275 280 285
Ala Leu Ala Ile Gly Gly Glu Thr Ile Gly Glu Thr Arg Ala Gly Asp
290 295 300
Pro Phe Ser Phe Thr Leu Asp Asn Ser Val Asn Ser Val Arg Phe Thr
305 310 315 320
Ala Ile Ala Lys Asp Asp Leu Gly Pro Asn Ala His Leu Val Leu Thr
325 330 335
Ala Pro Asn Gly Glu Thr Val Glu Leu Lys Asp Ser Gly Ser Ser Val
340 345 350
Ala Asn Ser Thr Asp Val Ser Trp Glu Ala Glu Ser Ser Pro Val Lys
355 360 365
Met Ala Asp Gly Ser Leu Asn Leu Gln Gln Gly Gly Asp Trp Lys Gly
370 375 380
Val Trp Gln Ile Gln Phe Gln Gly Ile Asp Pro Ala Ala Val Asp Gly
385 390 395 400
Arg Val Phe Asn Ser Val Glu Ile Gln Pro Asp Leu Gln Leu Val Phe
405 410 415
Ser Gly Gly Asp Ser Thr Ser Gly Ala Leu Asn Leu Arg Asp Asp Gln
420 425 430
Gln Leu Asn Met Gln Leu Val Gly Arg Asp Gly Gln Pro Arg Ile Leu
435 440 445
Glu Gly Ser Ala Leu Val Asp Leu Gly Phe Thr Arg Ala Asp Thr Gly
450 455 460
Glu Phe Ala Pro Leu Ala Gln Gly Ile Asp Ile Ser Gly Gly Glu Leu
465 470 475 480
Ser Phe Pro Leu Asp Thr Ile Ser Gln Leu Pro Ala Ile Gly Thr Val
485 490 495
Glu Ala Arg Thr Thr Ile Thr Thr Ala Gly Val Asp Asp Leu Pro Gly
500 505 510
Thr Thr Leu Ser Pro Ile Leu Asn Thr Thr Arg Ile Thr Ile Thr Gln
515 520 525
Arg Asp Met Pro Gln Leu Pro Ala Ser Val Arg Phe Thr Ala Asp Glu
530 535 540
Asp Val Val Thr Val Asp Ile Pro Ile Thr Gly Pro Gly Lys Val Trp
545 550 555 560
Ile Ala Pro Gly Thr Gln Leu Ser Gly Val Leu Pro Asp Gly Val Asp
565 570 575
Gly Ile Ala Ala Ser Ser Thr Phe Asp Ser Pro Asp Asn Ala Leu Val
580 585 590
Leu Gly Leu Asp Glu Gln Gly Thr Ile Pro Val Glu Leu Thr Val Ser
595 600 605
Asp Leu Arg Asp Gly Leu Val Asn Gly Ser Ile Pro Leu Gln Ile Ser
610 615 620
Asn Ala Glu Gly Ala Asn Glu Thr Ser Val Asp Leu Pro Thr Glu Gly
625 630 635 640
Thr Leu Ser Val Pro Ile Asn Ala Ser Thr Phe Ala Leu Ala Phe Ile
645 650 655
Leu Ala Leu Val Leu Ser Leu Leu Ile Pro Leu Leu Ile Leu Tyr Ile
660 665 670
Val Arg Phe Leu Ser Ala Lys Val Pro Ser Ser Ala Met Ser Gly Val
675 680 685
Arg Ile Pro Val Glu Phe Ser Gly Glu Ala Leu Arg Tyr Ala Gly Ser
690 695 700
Thr Met Pro Asp Leu Ala Ser Gln Thr Thr Ala Thr Lys Gln Val Val
705 710 715 720
Val His Gly Asp Thr Phe Asn Val Glu Gly His Lys Leu Lys Val Gln
725 730 735
Arg Phe Gln Leu Asn Pro Ile Ala Ser Pro Ala Val Ile Val Gln Thr
740 745 750
Asp Pro Ser Ile Ser Phe Asp Gly Lys Gln Lys Gly Thr Gln Ala Lys
755 760 765
Leu Pro Leu Ala Val Gln Gly Ser Trp Phe Leu Thr Ala Ser Gly Ala
770 775 780
Asp Pro Ser Lys Met Glu Leu Ile Ala Leu Thr Asn Leu Pro Leu Glu
785 790 795 800
Gln Gly Gln Ile Asp Arg Met Ile Ala Gly Ile Thr Ser Lys Ala Pro
805 810 815
Asp Arg Ala Arg Glu Leu Gln Lys Leu Leu Asp Asp Ala Ala Thr Ser
820 825 830
Gln Pro Ala Lys Val Pro Pro Arg Ala Pro Ala Ala Gln Gly His Val
835 840 845
Glu Lys Gln Ala Pro Ser Phe Gly Thr Gly Ser Gly Gly Gly Phe Gly
850 855 860
Ser Ser Asn Gly Gly Gly Phe Gly Ser Gly Ser Gly Ser Asn Asp Thr
865 870 875 880
Asr Gly Gly Phe Gly Ser Ser Gly Gly Phe Gly Ala Arg
885 890
<210>97
<211>1042
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1012)
<223>RXA00943
<400>97
taggtacgat cggacgagga gaaaaaatct gaaaacaatc cagtggccca cctcggctta 60
taaaactgga caacagtatt ttgattggag catcaccata atg atc cgc aaa ctt 115
Met Ile Arg Lys Leu
l 5
gct cga cca atg ctt gca tcg gtc tac gtc gca gat ggc gca gaa aca 163
Ala Arg Pro Met Leu Ala Ser Val Tyr Val Ala Asp Gly Ala Glu Thr
10 15 20
gta ttg aac acc agc gca cac gtc gaa ggc act cag gta gtt ctg gat 211
Val Leu Asn Thr Ser Ala His Val Glu Gly Thr Gln Val Val Leu Asp
25 30 35
cgt atc cgt tat gtg ctg ccc cgt aag tac gca aag cgc att tcc aga 259
Arg Ile Arg Tyr Val Leu Pro Arg Lys Tyr Ala Lys Arg Ile Ser Arg
40 45 50
gat cca gaa ttg gtc acc cgc gtc att ggc ggc acc aaa gtc ggt gcg 307
Asp Pro Glu Leu Val Thr Arg Val Ile Gly Gly Thr Lys Val Gly Ala
55 60 65
ggt tct ttg cta gct att ggt cgt gca cca cgc acc tct gca gct acc 355
Gly Ser Leu Leu Ala Ile Gly Arg Ala Pro Arg Thr Ser Ala Ala Thr
70 75 80 85
ctc gca atc ctg act atc cct aac atc ctg gct cgc aat gcg ttc tgg 403
Leu Ala Ile Leu Thr Ile Pro Asn Ile Leu Ala Arg Asn Ala Phe Trp
90 95 100
gaa acc cag gat gcg gat gaa aag cgt aac cgc cgc aac ggt ttc ctc 451
Glu Thr Gln Asp Ala Asp Glu Lys Arg Asn Arg Arg Asn Gly Phe Leu
105 110 115
acc aac att gcc ctg ctt ggt ggc ctg ttt atc act tct gtt gat act 499
Thr Asn Ile Ala Leu Leu Gly Gly Leu Phe Ile Thr Ser Val Asp Thr
120 125 130
gag ggc aag cct ggc gtg aag tgg cgt gca acc aat gct aca aag cgt 547
Glu Gly Lys Pro Gly Val Lys Trp Arg Ala Thr Asn Ala Thr Lys Arg
135 140 145
ggc aag aag cag ctg cag cag gca ctt cca acc aaa tct gag act gaa 595
Gly Lys Lys Gln Leu Gln Gln Ala Leu Pro Thr Lys Ser Glu Thr Glu
150 155 160 165
aag ttc ggt gag aag gcc tct gat tgg ttc aac gat act tct gac aag 643
Lys Phe Gly Glu Lys Ala Ser Asp Trp Phe Asn Asp Thr Ser Asp Lys
170 175 180
gtc acc gag tac gcg tac acc gct cag gat ttt gtc ggt gag aac aag 691
Val Thr Glu Tyr Ala Tyr Thr Ala Gln Asp Phe Val Gly Glu Asn Lys
185 190 195
gat gac tgg atc aag tcc gca acc gag act gct cac aag gtc gct gat 739
Asp Asp Trp Ile Lys Ser Ala Thr Glu Thr Ala His Lys Val Ala Asp
200 205 2l0
act gtg agc gat tac gct cac aag gct acc tct tac ctt gag gag aac 787
Thr Val Ser Asp Tyr Ala His Lys Ala Thr Ser Tyr Leu Glu Glu Asn
215 220 225
agc ggt gac tgg ctt gag gct gcg cag gct aac gcc aag act gct cgt 835
Ser Gly Asp Trp Leu Glu Ala Ala Gln Ala Asn Ala Lys Thr Ala Arg
230 235 240 245
aag tct gca gtg aag gct gcc ggc aag gct cag gaa aag gct aac ttt 883
Lys Ser Ala Val Lys Ala Ala Gly Lys Ala Gln Glu Lys Ala Asn Phe
250 255 260
gct ctt cag gtc gca gag gaa acc tct ggt cgc gcc aac aag aag gca 931
Ala Leu Gln Val Ala Glu Glu Thr Ser Gly Arg Ala Asn Lys Lys Ala
265 270 275
act aag agc tac gac aag ctt cag aag cag gct gat aag gcc atc gat 979
Thr Lys Ser Tyr Asp Lys Leu Gln Lys Gln Ala Asp Lys Ala Ile Asp
280 285 290
cgt gca cag aag aag ctg aag ggc atc gaa ctt taagtaactt cttcctgatt 1032
Arg Ala Gln Lys Lys Leu Lys Gly Ile Glu Leu
295 300
gagaaaaaat 1042
<210>98
<211>304
<212>PRT
<213>谷氨酸棒状杆菌
<400>98
Met Ile Arg Lys Leu Ala Arg Pro Met Leu Ala Ser Val Tyr Val Ala
1 5 10 15
Asp Gly Ala Glu Thr Val Leu Asn Thr Ser Ala His Val Glu Gly Thr
20 25 30
Gln Val Val Leu Asp Arg Ile Arg Tyr Val Leu Pro Arg Lys Tyr Ala
35 40 45
Lys Arg Ile Ser Arg Asp Pro Glu Leu Val Thr Arg Val Ile Gly Gly
50 55 60
Thr Lys Val Gly Ala Gly Ser Leu Leu Ala Ile Gly Arg Ala Pro Arg
65 70 75 80
Thr Ser Ala Ala Thr Leu Ala Ile Leu Thr Ile Pro Asn Ile Leu Ala
85 90 95
Arg Asn Ala Phe Trp Glu Thr Gln Asp Ala Asp Glu Lys Arg Asn Arg
100 105 110
Arg Asn Gly Phe Leu Thr Asn Ile Ala Leu Leu Gly Gly Leu Phe Ile
115 120 125
Thr Ser Val Asp Thr Glu Gly Lys Pro Gly Val Lys Trp Arg Ala Thr
130 135 140
Asn Ala Thr Lys Arg Gly Lys Lys Gln Leu Gln Gln Ala Leu Pro Thr
145 150 155 160
Lys Ser Glu Thr Glu Lys Phe Gly Glu Lys Ala Ser Asp Trp Phe Asn
165 170 175
Asp Thr Ser Asp Lys Val Thr Glu Tyr Ala Tyr Thr Ala Gln Asp Phe
180 185 190
Val Gly Glu Asn Lys Asp Asp Trp Ile Lys Ser Ala Thr Glu Thr Ala
195 200 205
His Lys Val Ala Asp Thr Val Ser Asp Tyr Ala His Lys Ala Thr Ser
210 215 220
Tyr Leu Glu Glu Asn Ser Gly Asp Trp Leu Glu Ala Ala Gln Ala Asn
225 230 235 240
Ala Lys Thr Ala Arg Lys Ser Ala Val Lys Ala Ala Gly Lys Ala Gln
245 250 255
Glu Lys Ala Asn Phe Ala Leu Gln Val Ala Glu Glu Thr Ser Gly Arg
260 265 270
Ala Asn Lys Lys Ala Thr Lys Ser Tyr Asp Lys Leu Gln Lys Gln Ala
275 280 285
Asp Lys Ala Ile Asp Arg Ala Gln Lys Lys Leu Lys Gly Ile Glu Leu
290 295 300
<210>99
<211>967
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(937)
<223>RXA00963
<400>99
ctggctctga cggcgtcgac ttgttctgct tctctgatac accaattttc gaggccctta 60
acctcgcacg tacttttact ccggaaggaa tctagaactt atg cgt ctt gca aca 115
Met Arg Leu Ala Thr
1 5
atc cgc acc aac ggc acc acc att gct gct cgt gtt gaa tct gaa aac 163
Ile Arg Thr Asn Gly Thr Thr Ile Ala Ala Arg Val Glu Ser Glu Asn
10 15 20
acc gct acc acc atc gag ggc ttt gcc aac gtc ggt gaa tta ctc cag 211
Thr Ala Thr Thr Ile Glu Gly Phe Ala Asn Val Gly Glu Leu Leu Gln
25 30 35
gaa tcc aac tgg cgc gag ctg gca gaa aac gct gct ggt gag gct gtg 259
Glu Ser Asn Trp Arg Glu Leu Ala Glu Asn Ala Ala Gly Glu Ala Val
40 45 50
acc ttt gaa aac aag gag cta gat gca gta gtt cca gca cct aag aag 307
Thr Phe Glu Asn Lys Glu Leu Asp Ala Val Val Pro Ala Pro Lys Lys
55 60 65
att gtg tgc gtc ggc ctt aac tac gcc aac cac att aaa gaa atg ggc 355
Ile Val Cys Val Gly Leu Asn Tyr Ala Asn His Ile Lys Glu Met Gly
70 75 80 85
cgc gac ctc cct gat acc cca acc ctt ttt gtt aag ttc cct gac gcg 403
Arg Asp Leu Pro Asp Thr Pro Thr Leu Phe Val Lys Phe Pro Asp Ala
90 95 100
ctc atc gga cct ttc gat gat gtt gtc gtt cca gag tgg gct aac aag 451
Leu Ile Gly Pro Phe Asp Asp Val Val Val Pro Glu Trp Ala Asn Lys
105 110 115
gct ctc gac tgg gaa ggc gag atg gca gtt atc att ggc aag cgc gca 499
Ala Leu Asp Trp Glu Gly Glu Met Ala Val Ile Ile Gly Lys Arg Ala
120 125 130
cgc cgt gtc aag cag gcc gat gct gct gag tac atc gct ggc tac gca 547
Arg Arg Val Lys Gln Ala Asp Ala Ala Glu Tyr Ile Ala Gly Tyr Ala
135 140 145
gtg atg aac gat tac acc acc cgc gat ttc cag tac gca gca cct gca 595
Val Met Asn Asp Tyr Thr Thr Arg Asp Phe Gln Tyr Ala Ala Pro Ala
150 155 160 165
aag act cca cag tgg cac cag ggc aag tct ttg gaa aag tcc gct ggc 643
Lys Thr Pro Gln Trp His Gln Gly Lys Ser Leu Glu Lys Ser Ala Gly
170 175 180
ttc ggg cct tgg atg act acc cca gat tct ttt gag ttc ggc ggc gag 691
Phe Gly Pro Trp Met Thr Thr Pro Asp Ser Phe Glu Phe Gly Gly Glu
185 190 195
ctg gca acc tac ctc gag ggc gag aag gta cag tcc acc cct acc aat 739
Leu Ala Thr Tyr Leu Glu Gly Glu Lys Val Gln Ser Thr Pro Thr Asn
200 205 210
gac ctg gtc ttt agc cca gaa aag ctc atc gaa tac atc acc cac atc 787
Asp Leu Val Phe Ser Pro Glu Lys Leu Ile Glu Tyr Ile Thr His Ile
215 220 225
tac cca ttg gat gct ggc gac gtc att gtc acc ggt acc cca ggc ggc 835
Tyr Pro Leu Asp Ala Gly Asp Val Ile Val Thr Gly Thr Pro Gly Gly
230 235 240 245
gtt ggc cac gca cgt aac cca cag cgc tac atc ggt gac ggc gaa acc 883
Val Gly His Ala Arg Asn Pro Gln Arg Tyr Ile Gly Asp Gly Glu Thr
250 255 260
gta aag gtt gag att gcg ggc ctc ggc ttc att gaa aac aag acg gtg 931
Val Lys Val Glu Ile Ala Gly Leu Gly Phe Ile Glu Asn Lys Thr Val
265 270 275
ttt gaa taaatgacaa ctttccacga tcttccgctg 967
Phe Glu
<210>100
<211>279
<212>PRT
<213>谷氨酸棒状杆菌
<400>100
Met Arg Leu Ala Thr Ile Arg Thr Asn Gly Thr Thr Ile Ala Ala Arg
1 5 10 15
Val Glu Ser Glu Asn Thr Ala Thr Thr Ile Glu Gly Phe Ala Asn Val
20 25 30
Gly Glu Leu Leu Gln Glu Ser Asn Trp Arg Glu Leu Ala Glu Asn Ala
35 40 45
Ala Gly Glu Ala Val Thr Phe Glu Asn Lys Glu Leu Asp Ala Val Val
50 55 60
Pro Ala Pro Lys Lys Ile Val Cys Val Gly Leu Asn Tyr Ala Asn His
65 70 75 80
Ile Lys Glu Met Gly Arg Asp Leu Pro Asp Thr Pro Thr Leu Phe Val
85 90 95
Lys Phe Pro Asp Ala Leu Ile Gly Pro Phe Asp Asp Val Val Val Pro
100 105 110
Glu Trp Ala Asn Lys Ala Leu Asp Trp Glu Gly Glu Met Ala Val Ile
115 120 125
Ile Gly Lys Arg Ala Arg Arg Val Lys Gln Ala Asp Ala Ala Glu Tyr
130 135 140
Ile Ala Gly Tyr Ala Val Met Asn Asp Tyr Thr Thr Arg Asp Phe Gln
145 150 155 160
Tyr Ala Ala Pro Ala Lys Thr Pro Gln Trp His Gln Gly Lys Ser Leu
165 170 175
Glu Lys Ser Ala Gly Phe Gly Pro Trp Met Thr Thr Pro Asp Ser Phe
180 185 190
Glu Phe Gly Gly Glu Leu Ala Thr Tyr Leu Glu Gly Glu Lys Val Gln
195 200 205
Ser Thr Pro Thr Asn Asp Leu Val Phe Ser Pro Glu Lys Leu Ile Glu
210 215 220
Tyr Ile Thr His Ile Tyr Pro Leu Asp Ala Gly Asp Val Ile Val Thr
225 230 235 240
Gly Thr Pro Gly Gly Val Gly His Ala Arg Asn Pro Gln Arg Tyr Ile
245 250 255
Gly Asp Gly Glu Thr Val Lys Val Glu Ile Ala Gly Leu Gly Phe Ile
260 265 270
Glu Asn Lys Thr Val Phe Glu
275
<210>101
<211>1363
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1333)
<223>RXA01011
<400>101
cccgcaaacc gatttcactc gccgactcat cgacgcgatc ccaggatcgc gctatcgtgg 60
tggcgaactc aatcttggac tataggagca gatcttaaaa atg tca gat ctc atc 115
Met Ser Asp Leu Ile
1 5
gac atg ctg gtt gga gaa gac ctc cca cag ctg cgt gac aac cgt ccc 163
Asp Met Leu Val Gly Glu Asp Leu Pro Gln Leu Arg Asp Asn Arg Pro
10 15 20
caa gct cgc gac aat gca caa aag agc ttc gaa gct ctg ctc gaa ccg 211
Gln Ala Arg Asp Asn Ala Gln Lys Ser Phe Glu Ala Leu Leu Glu Pro
25 30 35
gtc aac cca gga acc ttc agc ttc ggc gag cgt tac gcc gtg gcc acc 259
Val Asn Pro Gly Thr Phe Ser Phe Gly Glu Arg Tyr Ala Val Ala Thr
40 45 50
tat gtt gca ggt ttg cac caa ttc gcg cca gct gta gat ctc tac caa 307
Tyr Val Ala Gly Leu His Gln Phe Ala Pro Ala Val Asp Leu Tyr Gln
55 60 65
gat ttg ctt ctc gac gac gcc cca acc acc ctg gca aac gcc gtc tcc 355
Asp Leu Leu Leu Asp Asp Ala Pro Thr Thr Leu Ala Asn Ala Val Ser
70 75 80 85
gat gcc atc gac gag ggc cta tcc gca ggc cca tac ggc acc tac cgt 403
Asp Ala Ile Asp Glu Gly Leu Ser Ala Gly Pro Tyr Gly Thr Tyr Arg
90 95 100
gaa cct gga ctt gag tcc gaa tca gag cca ggc gga tct gtg cgc aac 451
Glu Pro Gly Leu Glu Ser Glu Ser Glu Pro Gly Gly Ser Val Arg Asn
105 110 115
gat gcc gcc aag ctt ggc gag cga ctt gca gca gca ttt gat tac gca 499
Asp Ala Ala Lys Leu Gly Glu Arg Leu Ala Ala Ala Phe Asp Tyr Ala
120 125 130
cac cta cta gtt ttc cac ccc cgt gat tcc cgc ccc gag gtc ctt ggt 547
His Leu Leu Val Phe His Pro Arg Asp Ser Arg Pro Glu Val Leu Gly
135 140 145
cga ctc tcc ggc gct ggc tgg agc gcg gat gac acc gtg aca ttg gct 595
Arg Leu Ser Gly Ala Gly Trp Ser Ala Asp Asp Thr Val Thr Leu Ala
150 155 160 165
cag ctg gtg tcg ttc ctg gca ttc cag ctg cgt gtt gcc tac ggc ctg 643
Gln Leu Val Ser Phe Leu Ala Phe Gln Leu Arg Val Ala Tyr Gly Leu
170 175 180
cgc acc ctc aaa ggt gaa gac att cag gtg aaa gct cct cgc ctg agt 691
Arg Thr Leu Lys Gly Glu Asp Ile Gln Val Lys Ala Pro Arg Leu Ser
185 190 195
att cct gaa gcg aag tgg gaa ctg tcc aac aac ggc ttt gag atc tcc 739
Ile Pro Glu Ala Lys Trp Glu Leu Ser Asn Asn Gly Phe Glu Ile Ser
200 205 210
acc tat gaa gaa ctc aac cgc cct gaa gcg ttt gtg aac cac tct ttg 787
Thr Tyr Glu Glu Leu Asn Arg Pro Glu Ala Phe Val Asn His Ser Leu
215 220 225
ggc tgg aag cca tgg gta cca cca gta gca aag gca gat ctc acc gsa 835
Gly Trp Lys Pro Trp Val Pro Pro Val Ala Lys Ala Asp Leu Thr Glu
230 235 240 245
gaa cag ctg gat tct ttg atc cag cct gag cgc gct gat atg cca tac 883
Glu Gln Leu Asp Ser Leu Ile Gln Pro Glu Arg Ala Asp Met Pro Tyr
250 255 260
ttc cgt ctc ttg gca cgc gat cct gca gca ttg aag gcg cgt acc ctg 931
Phe Arg Leu Leu Ala Arg Asp Pro Ala Ala Leu Lys Ala Arg Thr Leu
265 270 275
acc gat ctg gat atc ttc ttc aac act gac ggc gaa ggc cta gga cgt 979
Thr Asp Leu Asp Ile Phe Phe Asn Thr Asp Gly Glu Gly Leu Gly Arg
280 285 290
gca gag cgt gaa ctc ggc gcc acc gtt act tcc cgc tac aac ggc tgc 1027
Ala Glu Arg Glu Leu Gly Ala Thr Val Thr Ser Arg Tyr Asn Gly Cys
295 300 305
gtg tac tgc gca tcc gtg cac gca gga cgt gct cag gaa gaa tcc ggt 1075
Val Tyr Cys Ala Ser Val His Ala Gly Arg Ala Gln Glu Glu Ser Gly
310 315 320 325
cga gct gat gat gtc aac gca ctg ttg gca agc atc gac tcc gat ctg 1123
Arg Ala Asp Asp Val Asn Ala Leu Leu Ala Ser Ile Asp Ser Asp Leu
330 335 340
ggt tct gac cag tgg aat gtc atc cgt gac gct gct cgc gca ctg agc 1171
Gly Ser Asp Gln Trp Asn Val Ile Arg Asp Ala Ala Arg Ala Leu Ser
345 350 355
tcc acc cct gct gct ttc aac cag gga tgc atc acc aag ctg cgt ggc 1219
Ser Thr Pro Ala Ala Phe Asn Gln Gly Cys Ile Thr Lys Leu Arg Gly
360 365 370
gtt gga ttc agc gac ctg cag atc gtg gac ttg atc aac tct gtg gcg 1267
Val Gly Phe Ser Asp Leu Gln Ile Val Asp Leu Ile Asn Ser Val Ala
375 380 385
ttc ttc aac tgg gcg aac cgc ctg atg ttg tca ctg ggt gaa ccc gaa 1315
Phe Phe Asn Trp Ala Asn Arg Leu Met Leu Ser Leu Gly Glu Pro Glu
390 395 400 405
gtg cca aag cgt ttc ctgtaaaacgcat aaccccgaat accccctgtt 1363
Val Pro Lys Arg Phe Leu
410
<210>102
<211>411
<212>PRT
<213>谷氨酸棒状杆菌
<400>102
Met Ser Asp Leu Ile Asp Met Leu Val Gly Glu Asp Leu Pro Gln Leu
1 5 10 15
Arg Asp Asn Arg Pro Gln Ala Arg Asp Asn Ala Gln Lys Ser Phe Glu
20 25 30
Ala Leu Leu Glu Pro Val Asn Pro Gly Thr Phe Ser Phe Gly Glu Arg
35 40 45
Tyr Ala Val Ala Thr Tyr Val Ala Gly Leu His Gln Phe Ala Pro Ala
50 55 60
Val Asp Leu Tyr Gln Asp Leu Leu Leu Asp Asp Ala Pro Thr Thr Leu
65 70 75 80
Ala Asn Ala Val Ser Asp Ala Ile Asp Glu Gly Leu Ser Ala Gly Pro
85 90 95
Tyr Gly Thr Tyr Arg Glu Pro Gly Leu Glu Ser Glu Ser Glu Pro Gly
100 105 110
Gly Ser Val Arg Asn Asp Ala Ala Lys Leu Gly Glu Arg Leu Ala Ala
115 120 125
Ala Phe Asp Tyr Ala His Leu Leu Val Phe His Pro Arg Asp Ser Arg
130 135 140
Pro Glu Val Leu Gly Arg Leu Ser Gly Ala Gly Trp Ser Ala Asp Asp
145 150 155 160
Thr Val Thr Leu Ala Gln Leu Val Ser Phe Leu Ala Phe Gln Leu Arg
165 170 175
Val Ala Tyr Gly Leu Arg Thr Leu Lys Gly Glu Asp Ile Gln Val Lys
180 185 190
Ala Pro Arg Leu Ser Ile Pro Glu Ala Lys Trp Glu Leu Ser Asn Asn
195 200 205
Gly Phe Glu Ile Ser Thr Tyr Glu Glu Leu Asn Arg Pro Glu Ala Phe
210 215 220
Val Asn His Ser Leu Gly Trp Lys Pro Trp Val Pro Pro Val Ala Lys
225 230 235 240
Ala Asp Leu Thr Glu Glu Gln Leu Asp Ser Leu Ile Gln Pro Glu Arg
245 250 255
Ala Asp Met Pro Tyr Phe Arg Leu Leu Ala Arg Asp Pro Ala Ala Leu
260 265 270
Lys Ala Arg Thr Leu Thr Asp Leu Asp Ile Phe Phe Asn Thr Asp Gly
275 280 285
Glu Gly Leu Gly Arg Ala Glu Arg Glu Leu Gly Ala Thr Val Thr Ser
290 295 300
Arg Tyr Asn Gly Cys Val Tyr Cys Ala Ser Val His Ala Gly Arg Ala
305 310 315 320
Gln Glu Glu Ser Gly Arg Ala Asp Asp Val Asn Ala Leu Leu Ala Ser
325 330 335
Ile Asp Ser Asp Leu Gly Ser Asp Gln Trp Asn Val Ile Arg Asp Ala
340 345 350
Ala Arg Ala Leu Ser Ser Thr Pro Ala Ala Phe Asn Gln Gly Cys Ile
355 360 365
Thr Lys Leu Arg Gly Val Gly Phe Ser Asp Leu Gln Ile Val Asp Leu
370 375 380
Ile Asn Ser Val Ala Phe Phe Asn Trp Ala Asr Arg Leu Met Leu Ser
385 390 395 400
Leu Gly Glu Pro Glu Val Pro Lys Arg Phe Leu
405 410
<210>103
<211>739
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(709)
<223>RXA01017
<400>103
gaaatttgag ggggcgctac ccttagaagg tgcgcaatga caccacgata gttcgcgcct 60
agtgtggatt gctagaaaac tttaagaaag aggaaataat atg gct caa aaa gta 115
Met Ala Gln Lys Val
1 5
acc ttc tgg ttc gat acc acc tgc cca ttc tgc tgg gtc acc tcc cgc 163
Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys Trp Val Thr Ser Arg
10 15 20
tgg att aag gaa gtc gaa caa gtc cgc gat att gaa atc cag tgg gtt 211
Trp Ile Lys Glu Val Glu Gln Val Arg Asp Ile Glu Ile Gln Trp Val
25 30 35
cca atg agc ctc gct gtc cta aac gaa ggc cgt gat ctc cca gag gat 259
Pro Met Ser Leu Ala Val Leu Asn Glu Gly Arg Asp Leu Pro Glu Asp
40 45 50
tac aag gag cgc atg aag gct gca tgg gga cca gca cgc gtt ttc gca 307
Tyr Lys Glu Arg Met Lys Ala Ala Trp Gly Pro Ala Arg Val Phe Ala
55 60 65
gct gtc gcc acc gac cat gct gac aag ctc ggc gac ctg tac acc gca 355
Ala Val Ala Thr Asp His Ala Asp Lys Leu Gly Asp Leu Tyr Thr Ala
70 75 80 85
atg ggt acc cgc atc cac aac gac ggt cgc gga cca atc gaa ggt tcc 403
Met Gly Thr Arg Ile His Asn Asp Gly Arg Gly Pro Ile Glu Gly Ser
90 95 100
ttc aat gat gtc atc gca gag gca ctt gaa gag gtc ggc cta gac gct 451
Phe Asn Asp Val Ile Ala Glu Ala Leu Glu Glu Val Gly Leu Asp Ala
105 110 115
gca ctt ggt gaa gtt gca gac acc acc gaa tgg gac gac gca ctt cgc 499
Ala Leu Gly Glu Val Ala Asp Thr Thr Glu Trp Asp Asp Ala Leu Arg
120 125 130
gca ttc cac cag acc gca atg gac gag gtc ggc aac gat gtc gga acc 547
Ala Phe His Gln Thr Ala Met Asp Glu Val Gly Asn Asp Val Gly Thr
135 140 145
cca gtg gtc aag ctc ggc gac acc gct ttc ttc ggc cca gtg ctc acc 595
Pro Val Val Lys Leu Gly Asp Thr Ala Phe Phe Gly Pro Val Leu Thr
150 155 160 165
cgc atc cca cgc ggc gag gaa gca gga gag atc ttc gac gct tcc ttc 643
Arg Ile Pro Arg Gly Glu Glu Ala Gly Glu Ile Phe Asp Ala Ser Phe
170 175 180
aag ctc gca agc tat ccc cac ttc ttt gaa atc aag cgc agc cgc act 691
Lys Leu Ala Ser Tyr Pro His Phe Phe Glu Ile Lys Arg Ser Arg Thr
185 190 195
gag aac cca cag ttc gac taattaacgc tgtctctgct tattaaacac 739
Glu Asn Pro Gln Phe Asp
200
<210>104
<211>203
<212>PRT
<213>谷氨酸棒状杆菌
<400>104
Met Ala Gln Lys Val Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys
1 5 10 15
Trp Val Thr Ser Arg Trp Ile Lys Glu Val Glu Gln Val Arg Asp Ile
20 25 30
Glu Ile Gln Trp Val Pro Met Ser Leu Ala Val Leu Asn Glu Gly Arg
35 40 45
Asp Leu Pro Glu Asp Tyr Lys Glu Arg Met Lys Ala Ala Trp Gly Pro
50 55 60
Ala Arg Val Phe Ala Ala Val Ala Thr Asp His Ala Asp Lys Leu Gly
65 70 75 80
Asp Leu Tyr Thr Ala Met Gly Thr Arg Ile His Asn Asp Gly Arg Gly
85 90 95
Pro Ile Glu Gly Ser Phe Asn Asp Val Ile Ala Glu Ala Leu Glu Glu
100 105 110
Val Gly Leu Asp Ala Ala Leu Gly Glu Val Ala Asp Thr Thr Glu Trp
115 120 125
Asp Asp Ala Leu Arg Ala Phe His Gln Thr Ala Met Asp Glu Val Gly
130 135 140
Asn Asp Val Gly Thr Pro Val Val Lys Leu Gly Asp Thr Ala Phe Phe
145 150 155 160
Gly Pro Val Leu Thr Arg Ile Pro Arg Gly Glu Glu Ala Gly Glu Ile
165 170 175
Phe Asp Ala Ser Phe Lys Leu Ala Ser Tyr Pro His Phe Phe Glu Ile
180 185 190
Lys Arg Ser Arg Thr Glu Asn Pro Gln Phe Asp
195 200
<210>105
<211>658
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(628)
<223>RXA01037
<400>105
cgactaaacc gcatcgcttc atggaaatca gccggcgtac agcgctatct caaccccact 60
atctcttaga accacacaca accaagaaag cagtcactac atg acc acc tca tca 115
Met Thr Thr Ser Ser
1 5
ccc ctt aag tcc aaa aat tcc ctt aac ggc gcg cag acc aca ttt gtc 163
Pro Leu Lys Ser Lys Asn Ser Leu Asn Gly Ala Gln Thr Thr Phe Val
10 15 20
cgc aat tgt gtt gca gac ggc cca acc gag gta aaa gcc acc att gat 211
Arg Asn Cys Val Ala Asp Gly Pro Thr Glu Val Lys Ala Thr Ile Asp
25 30 35
gca cgt ggc ggt atc gaa cag ctc cgt gac aat gag att tca cag ctc 259
Ala Arg Gly Gly Ile Glu Gln Leu Arg Asp Asn Glu Ile Ser Gln Leu
40 45 50
ccc gac aag ctg tgg ttc aat agc gca acc tcc gca tcc aat gac gag 307
Pro Asp Lys Leu Trp Phe Asn Ser Ala Thr Ser Ala Ser Asn Asp Glu
55 60 65
ttc acc acc gat aac atc cga ctt ggg ttg ctc tac aac atg gca ctg 355
Phe Thr Thr Asp Asn Ile Arg Leu Gly Leu Leu Tyr Asn Met Ala Leu
70 75 80 85
aat aag gca tcg gct cat cgc tat gag ctt gct gcc cgc tgg tac gcc 403
Asn Lys Ala Ser Ala His Arg Tyr Glu Leu Ala Ala Arg Trp Tyr Ala
90 95 100
att gtt agc cct cag cca tcg caa gaa gct agc cac aag cgc atc gcg 451
Ile Val Ser Pro Gln Pro Ser Gln Glu Ala Ser His Lys Arg Ile Ala
105 110 115
tcc tgg cac ctt acc cag ctg cac gca ctg gtg acc cta tct cgc acc 499
Ser Trp His Leu Thr Gln Leu His Ala Leu Val Thr Leu Ser Arg Thr
120 125 130
cta ggg gtc atc att cct ggt gat gag atc gac gct cta gcg cag tct 547
Leu Gly Val Ile Ile Pro Gly Asp Glu Ile Asp Ala Leu Ala Gln Ser
135 140 145
atg gag aat gct cgt caa gtt ggc acc gag aat tgg tcc act gtc acc 595
Met Glu Asn Ala Arg Gln Val Gly Thr Glu Asn Trp Ser Thr Val Thr
150 155 160 165
atg cgc acc tac gtg acc aac cgc aag gca tcc taatgactcg cgatattttc 648
Met Arg Thr Tyr Val Thr Asn Arg Lys Ala Ser
170 175
cacgaggctg 658
<210>106
<211>176
<212>PRT
<213>谷氨酸棒状杆菌
<400>106
Met Thr Thr Ser Ser Pro Leu Lys Ser Lys Asn Ser Leu Asn Gly Ala
1 5 10 15
Gln Thr Thr Phe Val Arg Asn Cys Val Ala Asp Gly Pro Thr Glu Val
20 25 30
Lys Ala Thr Ile Asp Ala Arg Gly Gly Ile Glu Gln Leu Arg Asp Asn
35 40 45
Glu Ile Ser Gln Leu Pro Asp Lys Leu Trp Phe Asn Ser Ala Thr Ser
50 55 60
Ala Ser Asn Asp Glu Phe Thr Thr Asp Asn Ile Arg Leu Gly Leu Leu
65 70 75 80
Tyr Asn Met Ala Leu Asn Lys Ala Ser Ala His Arg Tyr G1u Leu Ala
85 90 95
Ala Arg Trp Tyr Ala Ile Val Ser Pro Gln Pro Ser Gln Glu Ala Ser
100 105 110
His Lys Arg Ile Ala Ser Trp His Leu Thr Gln Leu His A1a Leu Val
115 120 125
Thr Leu Ser Arg Thr Leu Gly Val Ile Ile Pro Gly Asp Glu Ile Asp
130 135 140
Ala Leu Ala Gln Ser Met Glu Asn Ala Arg Gln Val Gly Thr Glu Asn
145 150 155 160
Trp Ser Thr Val Thr Met Arg Thr Tyr Val Thr Asn Arg Lys Ala Ser
165 170 175
<210>107
<211>2038
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2008)
<223>RXA01046
<400>107
actcggcgta cacggaaaat agcagctgat ccatctaggg tcagtgaact ctcgcggatt 60
gccacacacc accaaccact acaagaaaga accccctacc gtg cct ttt cac gat 115
Val Pro Phe His Asp
1 5
ttc act gac cct gat agc gat cct agg caa aat gca cca gct aac ccc 163
Phe Thr Asp Pro Asp Ser Asp Pro Arg Gln Asn Ala Pro Ala Asn Pro
10 15 20
acc gct caa ccg cca tct gcc ccc acc tca cac tca tca cag cag ggt 211
Thr Ala Gln Pro Pro Ser Ala Pro Thr Ser His Ser Ser Gln Gln Gly
25 30 35
ctc cct ccg ggc gcc att atg atc cct ttt cca ggg caa ccg cag cag 259
Leu Pro Pro Gly Ala Ile Met Ile Pro Phe Pro Gly Gln Pro Gln Gln
40 45 50
caa agc gca ccc aat gac gag acc cgt ttc atc gac ctt aac gaa cgt 307
Gln Ser Ala Pro Asn Asp Glu Thr Arg Phe Ile Asp Leu Asn Glu Arg
55 60 65
cat aaa gat gat gaa cca gcc ctg ttt cgc gat gat gtt att gat caa 355
His Lys Asp Asp Glu Pro Ala Leu Phe Arg Asp Asp Val Ile Asp Gln
70 75 80 85
act ctc gct att ttg atc agt aaa aat aag ccc aat gcg cta ctc gtt 403
Thr Leu Ala Ile Leu Ile Ser Lys Asn Lys Pro Asn Ala Leu Leu Val
90 95 100
ggg cct gcc ggt aca ggt aaa tcc cgt atc gca gaa gat att gcg cgc 451
Gly Pro Ala Gly Thr Gly Lys Ser Arg Ile Ala Glu Asp Ile Ala Arg
105 110 115
cgc ctt gcc aat gat gac gta tct att ccc gat cag ctt gtc ggc cac 499
Arg Leu Ala Asn Asp Asp Val Ser Ile Pro Asp Gln Leu Val Gly His
120 125 130
cgt att ctt gat gtc tcc att gca gag ctt gtt gct ggt gct ggc gtt 547
Arg Ile Leu Asp Val Ser Ile Ala Glu Leu Val Ala Gly Ala Gly Val
135 140 145
gtt ggt cag ctc gaa gaa cgc att ctg gat ctc atc aag tat gcg acc 595
Val Gly Gln Leu Glu Glu Arg Ile Leu Asp Leu Ile Lys Tyr Ala Thr
150 155 160 165
gac ccg agt aac aaa gtc att atc ttt att gac gag att cac caa att 643
Asp Pro Ser Asn Lys Val Ile Ile Phe Ile Asp Glu Ile His Gln Ile
170 175 180
gct ggt gat cag tcc agt cac agt gga tcg caa gcc aaa gtt gct cag 691
Ala Gly Asp Gln Ser Ser His Ser Gly Ser Gln Ala Lys Val Ala Gln
185 190 195
att ctc aaa ccc tat ctt gcc cgt ggt gac ctt cgt gtt att ggt gcc 739
Ile Leu Lys Pro Tyr Leu Ala Arg Gly Asp Leu Arg Val Ile Gly Ala
200 205 210
acc acc acc cag gaa gct cgt gac ttc gat cat gat cca gcc ctc aaa 787
Thr Thr Thr Gln Glu Ala Arg Asp Phe Asp His Asp Pro Ala Leu Lys
215 220 225
cgc cgt ttt agc aga gta aat gtc gat gaa ttt gat cga gat caa acg 835
Arg Arg Phe Ser Arg Val Asn Val Asp Glu Phe Asp Arg Asp Gln Thr
230 235 240 245
ctc act att ctt cat gct gca cgt gat ggt tac ctc aaa cat ttc aac 883
Leu Thr Ile Leu His Ala Ala Arg Asp Gly Tyr Leu Lys His Phe Asn
250 255 260
aac gct gtc acg gta tct gac gac gta ctg ggc tat gtc tac acc tac 931
Asn Ala Val Thr Val Ser Asp Asp Val Leu Gly Tyr Val Tyr Thr Tyr
265 270 275
tcg cag caa ttc aac cca ggc aat aca gca caa cct gat gca gca ctg 979
Ser Gln Gln Phe Asn Pro Gly Asn Thr Ala Gln Pro Asp Ala Ala Leu
280 285 290
acg ctg ttt gat aag gcg ttg gct tcc cta act atg gag aaa cag cgt 1027
Thr Leu Phe Asp Lys Ala Leu Ala Ser Leu Thr Met Glu Lys Gln Arg
295 300 305
ctg atc aac aac cat gtc att gcg ccg tcg ctc aag ttc cct gtg tca 1075
Leu Ile Asn Asn His Val Ile Ala Pro Ser Leu Lys Phe Pro Val Ser
310 315 320 325
gaa agg cac atc cat aac acc gct cgc aaa ctt gcc ttt ggc tct caa 1123
Glu Arg His Ile His Asn Thr Ala Arg Lys Leu Ala Phe Gly Ser Gln
330 335 340
gtg cca gcc tcc atc aat act gat gat gct cgt gac aaa ctc gaa acg 1171
Val Pro Ala Ser Ile Asn Thr Asp Asp Ala Arg Asp Lys Leu Glu Thr
345 350 355
ttg ttt ggt caa gat cat att att gag cca gta ctc acc gct atc aag 1219
Leu Phe Gly Gln Asp His Ile Ile Glu Pro Val Leu Thr Ala Ile Lys
360 365 370
cgt gaa cag ctt ggt att ttc cct cgc acc aaa cca ttg agc tgg gtg 1267
Arg Glu Gln Leu Gly Ile Phe Pro Arg Thr Lys Pro Leu Ser Trp Val
375 380 385
ttt gct ggt tca tct ggt gtg ggt aaa aca gaa atg gcg cgt att ctc 1315
Phe Ala Gly Ser Ser Gly Val Gly Lys Thr Glu Met Ala Arg Ile Leu
390 395 400 405
tct cgc gcc att aat ggc ggc gat ccc atc att atc aat ggt ccc gaa 1363
Ser Arg Ala Ile Asn Gly Gly Asp Pro Ile Ile Ile Asn Gly Pro Glu
410 415 420
tac att agt cct gag tcc att act ggc ctt atc gga tca tcc gat ggc 1411
Tyr Ile Ser Pro Glu Ser Ile Thr Gly Leu Ile Gly Ser Ser Asp Gly
425 430 435
tat atc ggc tct aat tct aag cgt gct aaa cca ctc gac ccg ctg att 1459
Tyr Ile Gly Ser Asn Ser Lys Arg Ala Lys Pro Leu Asp Pro Leu Ile
440 445 450
tct aat ccg cgt cag gtg att gtg ctc gat gaa ttt gag aag tct cac 1507
Ser Asn Pro Arg Gln Val Ile Val Leu Asp Glu Phe Glu Lys Ser His
455 460 465
cct cat ttc cag caa ttg ttc atg gca gct ctt gat aca ggc act atg 1555
Pro His Phe Gln Gln Leu Phe Met Ala Ala Leu Asp Thr Gly Thr Met
470 475 480 485
gcg atg gct aat ggc acg aca ttg aat ttc tct cag gcc att atc att 1603
Ala Met Ala Asn Gly Thr Thr Leu Asn Phe Ser Gln Ala Ile Ile Ile
490 495 500
gcc acc acc aat gca gcc cgc gac aaa atc ggt cgt gac agc ttt gga 1651
Ala Thr Thr Asn Ala Ala Arg Asp Lys Ile Gly Arg Asp Ser Phe Gly
505 510 515
ttc gat tca gat aat tca ggt gtc ctc ggt tct gct caa gca gca act 1699
Phe Asp Ser Asp Asn Ser Gly Val Leu Gly Ser Ala Gln Ala Ala Thr
520 525 530
gat ccg cgt gca cag gaa cgc ctc aag tca ctg atg tcc aag gat ttc 1747
Asp Pro Arg Ala Gln Glu Arg Leu Lys Ser Leu Met Ser Lys Asp Phe
535 540 545
cct gtt gaa ctg ctc aac cgt ttc cag aat atc ttt gcc ttc aac cgc 1795
Pro Val Glu Leu Leu Asn Arg Phe Gln Asn Ile Phe Ala Phe Asn Arg
550 555 560 565
att gat gca ggc acc tac cgt gag att ctg gac aat ctc tac cag cgt 1843
Ile Asp Ala Gly Thr Tyr Arg Glu Ile Leu Asp Asn Leu Tyr Gln Arg
570 575 580
cgc cgt gac gcc gtg ctg ctt agc cac ccg cat tac gca gca cag atc 1891
Arg Arg Asp Ala Val Leu Leu Ser His Pro His Tyr Ala Ala Gln Ile
585 590 595
cct gca gat att gat tca gac act ctt gat cag ctg gtg gaa acc acc 1939
Pro Ala Asp Ile Asp Ser Asp Thr Leu Asp Gln Leu Val Glu Thr Thr
600 605 610
ttt atc tca gat ttt ggt gca cgt cct gct gca cgc acc atc gaa gac 1987
Phe Ile Ser Asp Phe Gly Ala Arg Pro Ala Ala Arg Thr Ile Glu Asp
615 620 625
cac atc gca tcc ttg ctg atg tgaccaacct tttaggagta catcatgatt 2038
His Ile Ala Ser Leu Leu Met
630 635
<210>108
<211>636
<212>PRT
<213>谷氨酸棒状杆菌
<400>108
Val Pro Phe His Asp Phe Thr Asp Pro Asp Ser Asp Pro Arg Gln Asn
1 5 10 15
Ala Pro Ala Asn Pro Thr Ala Gln Pro Pro Ser Ala Pro Thr Ser His
20 25 30
Ser Ser Gln Gln Gly Leu Pro Pro Gly Ala Ile Met Ile Pro Phe Pro
35 40 45
Gly Gln Pro Gln Gln Gln Ser Ala Pro Asn Asp Glu Thr Arg Phe Ile
50 55 60
Asp Leu Asn Glu Arg His Lys Asp Asp Glu Pro Ala Leu Phe Arg Asp
65 70 75 80
Asp Val Ile Asp Gln Thr Leu Ala Ile Leu Ile Ser Lys Asn Lys Pro
85 90 95
Asn Ala Leu Leu Val Gly Pro Ala Gly Thr Gly Lys Ser Arg Ile Ala
100 105 110
Glu Asp Ile Ala Arg Arg Leu Ala Asn Asp Asp Val Ser Ile Pro Asp
115 120 125
Gln Leu Val Gly His Arg Ile Leu Asp Val Ser Ile Ala Glu Leu Val
130 135 140
Ala Gly Ala Gly Val Val Gly Gln Leu Glu Glu Arg Ile Leu Asp Leu
145 150 155 160
Ile Lys Tyr Ala Thr Asp Pro Ser Asn Lys Val Ile Ile Phe Ile Asp
165 170 175
Glu Ile His Gln Ile Ala Gly Asp Gln Ser Ser His Ser Gly Ser Gln
180 185 190
Ala Lys Val Ala Gln Ile Leu Lys Pro Tyr Leu Ala Arg Gly Asp Leu
195 200 205
Arg Val Ile Gly Ala Thr Thr Thr Gln Glu Ala Arg Asp Phe Asp His
210 215 220
Asp Pro Ala Leu Lys Arg Arg Phe Ser Arg Val Asn Val Asp Glu Phe
225 230 235 240
Asp Arg Asp Gln Thr Leu Thr Ile Leu His Ala Ala Arg Asp Gly Tyr
245 250 255
Leu Lys His Phe Asn Asn Ala Val Thr Val Ser Asp Asp Val Leu Gly
260 265 270
Tyr Val Tyr Thr Tyr Ser Gln Gln Phe Asn Pro Gly Asn Thr Ala Gln
275 280 285
Pro Asp Ala Ala Leu Thr Leu Phe Asp Lys Ala Leu Ala Ser Leu Thr
290 295 300
Met Glu Lys Gln Arg Leu Ile Asn Asn His Val Ile Ala Pro Ser Leu
305 310 315 320
Lys Phe Pro Val Ser Glu Arg His Ile His Asn Thr Ala Arg Lys Leu
325 330 335
Ala Phe Gly Ser Gln Val Pro Ala Ser Ile Asn Thr Asp Asp Ala Arg
340 345 350
Asp Lys Leu Glu Thr Leu Phe Gly Gln Asp His Ile Ile Glu Pro Val
355 360 365
Leu Thr Ala Ile Lys Arg Glu Gln Leu Gly Ile Phe Pro Arg Thr Lys
370 375 380
Pro Leu Ser Trp Val Phe Ala Gly Ser Ser Gly Val Gly Lys Thr Glu
385 390 395 400
Met Ala Arg Ile Leu Ser Arg Ala Ile Asn Gly Gly Asp Pro Ile Ile
405 410 415
Ile Asn Gly Pro Glu Tyr Ile Ser Pro Glu Ser Ile Thr Gly Leu Ile
420 425 430
Gly Ser Ser Asp Gly Tyr Ile Gly Ser Asn Ser Lys Arg Ala Lys Pro
435 440 445
Leu Asp Pro Leu Ile Ser Asn Pro Arg Gln Val Ile Val Leu Asp Glu
450 455 460
Phe Glu Lys Ser His Pro His Phe Gln Gln Leu Phe Met Ala Ala Leu
465 470 475 480
Asp Thr Gly Thr Met Ala Met Ala Asn Gly Thr Thr Leu Asn Phe Ser
485 490 495
Gln Ala Ile Ile Ile Ala Thr Thr Asn Ala Ala Arg Asp Lys Ile Gly
500 505 510
Arg Asp Ser Phe Gly Phe Asp Ser Asp Asn Ser Gly Val Leu Gly Ser
515 520 525
Ala Gln Ala Ala Thr Asp Pro Arg Ala Gln Glu Arg Leu Lys Ser Leu
530 535 540
Met Ser Lys Asp Phe Pro Val Glu Leu Leu Asn Arg Phe Gln Asn Ile
545 550 555 560
Phe Ala Phe Asn Arg Ile Asp Ala Gly Thr Tyr Arg Glu Ile Leu Asp
565 570 575
Asn Leu Tyr Gln Arg Arg Arg Asp Ala Val Leu Leu Ser His Pro His
580 585 590
Tyr Ala Ala Gln Ile pro Ala Asp Ile Asp Ser Asp Thr Leu Asp Gln
595 600 605
Leu Val Glu Thr Thr Phe Ile Ser Asp Phe Gly Ala Arg Pro Ala Ala
610 615 620
Arg Thr Ile Glu Asp His Ile Ala Ser Leu Leu Met
625 630 635
<210>109
<211>772
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(742)
<223>RXA01109
<400>109
gggtcgtcag ctagattgct ttgctagggt aatctgcgtg aaccgtccgg gaatttttaa 60
gttttccgga gcatgtcgat gatttatcag gagcagaact ttg tct att agc ctt 115
Leu Ser Ile Ser Leu
1 5
tcc tca aag aag gtt gct ttc gca gca ctc atg gtc acc cca ctt ctc 163
Ser Ser Lys Lys Val Ala Phe Ala Ala Leu Met Val Thr Pro Leu Leu
10 15 20
ttg acc gca tgt ggc agt gat tca tct gat act gaa gca gcc tca tcc 211
Leu Thr Ala Cys Gly Ser Asp Ser Ser Asp Thr Glu Ala Ala Ser Ser
25 30 35
agc gca gcg acg acg aca aat tct tcc tca tcc agc gca gcg acc tcg 259
Ser Ala Ala Thr Thr Thr Asn Ser Ser Ser Ser Ser Ala Ala Thr Ser
40 45 50
gcg gaa gca gca gaa acc acc tcg tcc gaa tct gaa tct tcc gaa gca 307
Ala Glu Ala Ala Glu Thr Thr Ser Ser Glu Ser Glu Ser Ser Glu Ala
55 60 65
aca acc atc aac gaa gag cag caa gca cag ctt gat gta ctg tct caa 355
Thr Thr Ile Asn Glu Glu Gln Gln Ala Gln Leu Asp Val Leu Ser Gln
70 75 80 85
gag ctg tct gag aac cca att acc ttc gct gaa gct gcg cca gtt gaa 403
Glu Leu Ser Glu Asn Pro Ile Thr Phe Ala Glu Ala Ala Pro Val Glu
90 95 100
aac ggc gag acc gct tcc cca gaa gac aca gct gcc atc gag gct ctg 451
Asn Gly Glu Thr Ala Ser Pro Glu Asp Thr Ala Ala Ile Glu Ala Leu
105 110 115
gtt cgc gga tac act gac acc aac acc ttg cgt agc tcc ctt gcg tac 499
Val Arg Gly Tyr Thr Asp Thr Asn Thr Leu Arg Ser Ser Leu Ala Tyr
120 125 130
acc atc aac aac acc tgc acc cgc gtt ctt gaa gcc agc ggc gct gac 547
Thr Ile Asn Asn Thr Cys Thr Arg Val Leu Glu Ala Ser Gly Ala Asp
135 140 145
gct acc cag ctt gat ctc aac acc atc cct gat atc cca ctg ggt ggc 595
Ala Thr Gln Leu Asp Leu Asn Thr Ile Pro Asp Ile Pro Leu Gly Gly
150 155 160 165
gaa ggc acc gga act gtt gat tcc atc acc gat gtt gtg gtc aac ggc 643
Glu Gly Thr Gly Thr Val Asp Ser Ile Thr Asp Val Val Val Asn Gly
170 175 180
cag gaa gca tcc gca tgg gtc gta gca acc gcc ggt gga acc acc gac 691
Gln Glu Ala Ser Ala Trp Val Val Ala Thr Ala Gly Gly Thr Thr Asp
185 190 195
tct gca acc cag cgc ttc ttc aac gaa ggt ggc cag tgg aag ttc tgc 739
Ser Ala Thr Gln Arg Phe Phe Asn Glu Gly Gly Gln Trp Lys Phe Cys
200 205 210
gactaatccacag agcttaaaac tccggttgtc 772
Asp
<210>110
<211>214
<212>PRT
<213>谷氨酸棒状杆菌
<400>110
Leu Ser Ile Ser Leu Ser Ser Lys Lys Val Ala Phe Ala Ala Leu Met
1 5 10 15
Val Thr Pro Leu Leu Leu Thr Ala Cys Gly Ser Asp Ser Ser Asp Thr
20 25 30
Glu Ala Ala Ser Ser Ser Ala Ala Thr Thr Thr Asn Ser Ser Ser Ser
35 40 45
Ser Ala Ala Thr Ser Ala Glu Ala Ala Glu Thr Thr Ser Ser Glu Ser
50 55 60
Glu Ser Ser Glu Ala Thr Thr Ile Asn Glu Glu Gln Gln Ala Gln Leu
65 70 75 80
Asp Val Leu Ser Gln Glu Leu Ser Glu Asn Pro Ile Thr Phe Ala Glu
85 90 95
Ala Ala Pro Val Glu Asn Gly Glu Thr Ala Ser Pro Glu Asp Thr Ala
100 105 110
Ala Ile Glu Ala Leu Val Arg Gly Tyr Thr Asp Thr Asn Thr Leu Arg
115 120 125
Ser Ser Leu Ala Tyr Thr Ile Asn Asn Thr Cys Thr Arg Val Leu Glu
130 135 140
Ala Ser Gly Ala Asp Ala Thr Gln Leu Asp Leu Asn Thr Ile Pro Asp
145 150 155 160
Ile Pro Leu Gly Gly Glu Gly Thr Gly Thr Val Asp SerIle Thr Asp
165 170 175
Val Val Val Asn Gly Gln Glu Ala Ser Ala Trp Val Val Ala Thr Ala
180 185 190
Gly Gly Thr Thr Asp Ser Ala Thr Gln Arg Phe Phe Asn Glu Gly Gly
195 200 205
Gln Trp Lys Phe Cys Asp
210
<210>111
<211>652
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(622)
<223>RXA01122
<400>111
tattcgacca aggctgagca aaaaggcgct aagctgtggg gagatcgcaa gcccctatcc 60
ggcctctact aagccccgaa gttgaaggga aactccccca atg ccg ttt cca gca 115
Met Pro Phe Pro Ala
1 5
ctg ttg ttg ccg ctg att ttc tgg aca gga att gca gct ttg tcc tct 163
Leu Leu Leu Pro Leu Ile Phe Trp Thr Gly Ile Ala Ala Leu Ser Ser
10 15 20
tgg gcg gtc agc cgt gca ctt ccg ctg cgc gcc gat aat tcc att gag 211
Trp Ala Val Ser Arg Ala Leu Pro Leu Arg Ala Asp Asn Ser Ile Glu
25 30 35
att gat gcg ccg gtg gaa aag gtc tgg gat ttc atc gag gag acc aac 259
Ile Asp Ala Pro Val Glu Lys Val Trp Asp Phe Ile Glu Glu Thr Asn
40 45 50
cgc gtg ccg gag tgg aat gag cac att ctg tac gtg cag gcg cct ggt 307
Arg Val Pro Glu Trp Asn Glu His Ile Leu Tyr Val Gln Ala Pro Gly
55 60 65
gag atc gag cag ggc atg aag ctc aag atg aaa aca agg cac cca gag 355
Glu Ile Glu Gln Gly Met Lys Leu Lys Met Lys Thr Arg His Pro Glu
70 75 80 85
acc aat cgc ctc act ttg aag ttt cgc ccc acc atc gac gtg ctg cgt 403
Thr Asn Arg Leu Thr Leu Lys Phe Arg Pro Thr Ile Asp Val Leu Arg
90 95 100
cca cac cgt gaa ttg acg tgg tcc acc aaa att gtt gcg cgt tgg ctg 451
Pro His Arg Glu Leu Thr Trp Ser Thr Lys Ile Val Ala Arg Trp Leu
105 110 115
ctc acc gtc acc gac acg atc gag ctg aag cct ttg gaa gat ggt cgc 499
Leu Thr Val Thr Asp Thr Ile Glu Leu Lys Pro Leu Glu Asp Gly Arg
120 125 130
act gag gtg gat caa tca atg tcc ttt agt ggc gta cta tcc ccc gga 547
Thr Glu Val Asp Gln Ser Met Ser Phe Ser Gly Val Leu Ser Pro Gly
135 140 145
gtg ccc ttt ttg gcc agc atc agc agg atc aaa gag aac tca aac cgc 595
Val Pro Phe Leu Ala Ser Ile Ser Arg Ile Lys Glu Asn Ser Asn Arg
150 155 160 165
cag ttg aaa gca ctg atc gaa gcc gag taaacctcca acccctacat 642
Gln Leu Lys Ala Leu Ile Glu Ala Glu
170
aacgtggggg 652
<210>112
<211>174
<212>PRT
<213>谷氨酸棒状杆菌
<400>112
Met Pro Phe Pro Ala Leu Leu Leu Pro Leu Ile Phe Trp Thr Gly Ile
1 5 10 15
Ala Ala Leu Ser Ser Trp Ala Val Ser Arg Ala Leu Pro Leu Arg Ala
20 25 30
Asp Asn Ser Ile Glu Ile Asp Ala Pro Val Glu Lys Val Trp Asp Phe
35 40 45
Ile Glu Glu Thr Asn Arg Val Pro Glu Trp Asn Glu His Ile Leu Tyr
50 55 60
Val Gln Ala Pro Gly Glu Ile Glu Gln Gly Met Lys Leu Lys Met Lys
65 70 75 80
Thr Arg His Pro Glu Thr Asn Arg Leu Thr Leu Lys Phe Arg Pro Thr
85 90 95
Ile Asp Val Leu Arg Pro His Arg Glu Leu Thr Trp Ser Thr Lys Ile
100 105 110
Val Ala Arg Trp Leu Leu Thr Val Thr Asp Thr Ile Glu Leu Lys Pro
115 120 125
Leu Glu Asp Gly Arg Thr Glu Val Asp Gln Ser Met Ser Phe Ser Gly
130 135 140
Val Leu Ser Pro Gly Val Pro Phe Leu Ala Ser Ile Ser Arg Ile Lys
145 150 155 160
Glu Asn Ser Asn Arg Gln Leu Lys Ala Leu Ile Glu Ala Glu
165 170
<210>113
<211>1171
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1141)
<223>RXA01127
<400>113
aacgatttcc agttgagtca agccctgcat gatggggtag tgggggttgttgggcaggta 60
cgagctgtga tcaatcagct acactagtga agtccatata gtg aga agg gaa tcc 115
Val Arg Arg Glu Ser
1 5
cac aac atg aaa ctt gct gtt att ggt gga gat ggt atc ggc cca gag 163
His Asn Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu
10 15 20
gtt act gca gaa gcc ctc aag gtt cta aac gct gtc cgc gac gac atc 211
Val Thr Ala Glu Ala Leu Lys Val Leu Asn Ala Val Arg Asp Asp Ile
25 30 35
gag acc acc gat tat gac ctt ggc gca cgc cgt tac ctc aaa aat ggc 259
Glu Thr Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Lys Asn Gly
40 45 50
gag ctg ctc acc gac gag gat ctg gca tcc ctg cgc gag cat gac gcg 307
Glu Leu Leu Thr Asp Glu Asp Leu Ala Ser Leu Arg Glu His Asp Ala
55 60 65
atc ctt ctt ggc gct atc ggt gca cca ggt tcc gtc cct cca gga att 355
Ile Leu Leu Gly Ala Ile Gly Ala Pro Gly Ser Val Pro Pro Gly Ile
70 75 80 85
ctc gag cgt ggt ttg ctg ctg aag atg cga ttc gca ctg gat cac cac 403
Leu Glu Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His
90 95 100
gtg aac ctg cgc cca tcc aag ctg tac gac ggc gtg gag tcc cca ctg 451
Val Asn Leu Arg Pro Ser Lys Leu Tyr Asp Gly Val Glu Ser Pro Leu
105 110 115
cgt aac cca ggc aag att gat ttc gtt gtg gtc cgc gaa ggt acc gaa 499
Arg Asn Pro Gly Lys Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu
120 125 130
ggc gcc tac act ggc aac ggt gga gca atc cgc gtg gga acc cct cac 547
Gly Ala Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His
135 140 145
gag att gcc aat gaa acc tcc gtg aac act cgc tac ggc gct gag cgc 595
Glu Ile Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg
150 155 160 165
gtt att cgc tac gca ttc gag ctg gca cag agc cgc cgc aag aag ctc 643
Val Ile Arg Tyr Ala Phe Glu Leu Ala Gln Ser Arg Arg Lys Lys Leu
170 175 180
acc ctc gtg cac aag acc aac gtc ctg gtt cac ggt ggt ggc ctg tgg 691
Thr Leu Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Leu Trp
185 190 195
cag cgc acc gta gat gag gtt gca aag gaa tac cca gag gta gcc gtc 739
Gln Arg Thr Val Asp Glu Val Ala Lys Glu Tyr Pro Glu Val Ala Val
200 205 210
gat tac aac cac atc gat gca gca acc atc tat ctg gtc act gat cct 787
Asp Tyr Asn His Ile Asp Ala Ala Thr Ile Tyr Leu Val Thr Asp Pro
215 220 225
tcc cgc ttc gat gtg att gtt acc gat aac ctc ttc ggc gac atc ctc 835
Ser Arg Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Leu
230 235 240 245
acc gat gag gca ggc gca gtc tct ggc gga att ggc ctc gca gca tcc 883
Thr Asp Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser
250 255 260
ggc aac atc gat gcc acg ggc acc aac cct tcc atg ttc gag cca gtc 931
Gly Asn Ile Asp Ala Thr Gly Thr Asn Pro Ser Met Phe Glu Pro Val
265 270 275
cac ggc tct gca cca gat atc gca ggc cag gga atc gca gac cca acg 979
His Gly Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr
280 285 290
gca gca atc cta tcc gct gcg atg ctg ctg cgt cac tta ggt gat gaa 1027
Ala Ala Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu
295 300 305
gac aac gca gta cgt att gaa aca gcc atc gca gct gat gtg gct ggc 1075
Asp Asn Ala Val Arg Ile Glu Thr Ala Ile Ala Ala Asp Val Ala Gly
310 315 320 325
cga gat aac tct cag ccg att tct acc act gag gtg gga gac cgc atc 1123
Arg Asp Asn Ser Gln Pro Ile Ser Thr Thr Glu Val Gly Asp Arg Ile
330 335 340
gtc aag gcg ctg caa agc taaatttcaa cgccgacccc cttgcctttt 1171
Val Lys Ala Leu Gln Ser
345
<210>114
<211>347
<212>PRT
<213>谷氨酸棒状杆菌
<400>114
Val Arg Arg Glu Ser His Asn Met Lys Leu Ala Val Ile Gly Gly Asp
1 5 10 15
Gly Ile Gly Pro Glu Val Thr Ala Glu Ala Leu Lys Val Leu Asn Ala
20 25 30
Val Arg Asp Asp Ile Glu Thr Thr Asp Tyr Asp Leu Gly Ala Arg Arg
35 40 45
Tyr Leu Lys Asn Gly Glu Leu Leu Thr Asp Glu Asp Leu Ala Ser Leu
50 55 60
Arg Glu His Asp Ala Ile Leu Leu Gly Ala Ile Gly Ala Pro Gly Ser
65 70 75 80
Val Pro Pro Gly Ile Leu Glu Arg Gly Leu Leu Leu Lys Met Arg Phe
85 90 95
Ala Leu Asp His His Val Asn Leu Arg Pro Ser Lys Leu Tyr Asp Gly
100 105 110
Val Glu Ser Pro Leu Arg Asn Pro Gly Lys Ile Asp Phe Val Val Val
115 120 125
Arg Glu Gly Thr Glu Gly Ala Tyr Thr Gly Asn Gly Gly Ala Ile Arg
130 135 140
Val Gly Thr Pro His Glu Ile Ala Asn Glu Thr Ser Val Asn Thr Arg
145 150 155 160
Tyr Gly Ala Glu Arg Val Ile Arg Tyr Ala Phe Glu Leu Ala Gln Ser
165 170 175
Arg Arg Lys Lys Leu Thr Leu Val His Lys Thr Asn Val Leu Val His
180 185 190
Gly Gly Gly Leu Trp Gln Arg Thr Val Asp Glu Val Ala Lys Glu Tyr
195 200 205
Pro Glu Val Ala Val Asp Tyr Asn His Ile Asp Ala Ala Thr Ile Tyr
210 215 220
Leu Val Thr Asp Pro Ser Arg Phe Asp Val Ile Val Thr Asp Asn Leu
225 230 235 240
Phe Gly Asp Ile Leu Thr Asp Glu Ala Gly Ala Val Ser Gly Gly Ile
245 250 255
Gly Leu Ala Ala Ser Gly Asn Ile Asp Ala Thr Gly Thr Asn Pro Ser
260 265 270
Met Phe Glu Pro Val His Gly Ser Ala Pro Asp Ile Ala Gly Gln Gly
275 280 285
Ile Ala Asp Pro Thr Ala Ala Ile Leu Ser Ala Ala Met Leu Leu Arg
290 295 300
His Leu Gly Asp Glu Asp Asn Ala Val Arg Ile Glu Thr Ala Ile Ala
305 310 315 320
Ala Asp Val Ala Gly Arg Asp Asn Ser Gln Pro Ile Ser Thr Thr Glu
325 330 335
Val Gly Asp Arg Ile Val Lys Ala Leu Gln Ser
340 345
<210>115
<211>1192
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1162)
<223>RXA01128
<400>115
tgagattaac cataaaagaa aaactgcctc attgtctcga aatcctgcat tcgagtacaa 60
ataaatttga gaacaaaaag atggggggaa atatgttaaa gtg cgt tat att gct 115
Val Arg Tyr Ile Ala
1 5
tta gcg aaa gta act tcc atg cat caa cgt tcg atg agc ttc act ttc 163
Leu Ala Lys Val Thr Ser Met His Gln Arg Ser Met Ser Phe Thr Phe
10 15 20
att cgt act ttt ttc gta ctt ttt ggc atc acg ttg tta gtg tca tgc 21l
Ile Arg Thr Phe Phe Val Leu Phe Gly Ile Thr Leu Leu Val Ser Cys
25 30 35
gtt cca gag cct cct gac tcc tac act aaa gaa tcc act gtg ctg cgg 259
Val Pro Glu Pro Pro Asp Ser Tyr Thr Lys Glu Ser Thr Val Leu Arg
40 45 50
tat cag gtc tct gat ttc aat cta aac ttc gtg gaa tta gca gtt gcg 307
Tyr Gln Val Ser Asp Phe Asn Leu Asn Phe Val Glu Leu Ala Val Ala
55 60 65
ctt ggg tat ttg aac aac att gag ctc caa gta gtc gga tct gta caa 355
Leu Gly Tyr Leu Asn Asn Ile Glu Leu Gln Val Val Gly Ser Val Gln
70 75 80 85
ggc ggc gtt gag tcc att gaa tcg ctc aaa aag gat gac att gac ttc 403
Gly Gly Val Glu Ser Ile Glu Ser Leu Lys Lys Asp Asp Ile Asp Phe
90 95 100
gcg gca gtc ccc ttc att ggc ctt gtt gca gga gag ata gcc acc ggt 451
Ala Ala Val Pro Phe Ile Gly Leu Val Ala Gly Glu Ile Ala Thr Gly
105 110 115
gcg ccc atc aaa gca gtg gcc gca agt tac gga att tcc cac gat tct 499
Ala Pro Ile Lys Ala Val Ala Ala Set Tyr Gly Ile Ser His Asp Ser
120 125 130
tct tct gca ctt cta gtc ctt aaa gac agt gag ata cac gaa gtg cac 547
Ser Ser Ala Leu Leu Val Leu Lys Asp Ser Glu Ile His Glu Val His
135 140 145
gat ctc att ggc aaa aca gtt ggc ata aac acc ctc ggt gct ctg gga 595
Asp Leu Ile Gly Lys Thr Val Gly Ile Asn Thr Leu Gly Ala Leu Gly
150 155 160 165
tct gcg atg gtt gag cgt cat cta ttc gac gcc ggt ctc acc gaa cct 643
Ser Ala Met Val Glu Arg His Leu Phe Asp Ala Gly Leu Thr Glu Pro
170 175 180
gag atc gtg agc gtc act caa cgt gca tta ccc ggt gag tac tta gaa 691
Glu Ile Val Ser Val Thr Gln Arg Ala Leu Pro Gly Glu Tyr Leu Glu
185 190 195
caa cgc ctc tac cag ggg caa gtt gat gca att tgg gtc acc gat agc 739
Gln Arg Leu Tyr Gln Gly Gln Val Asp Ala Ile Trp Val Thr Asp Ser
200 205 210
gct aaa cac caa gcg ctt gaa act gga gat ttt cgg atc ttg gca gag 787
Ala Lys His Gln Ala Leu Glu Thr Gly Asp Phe Arg Ile Leu Ala Glu
215 220 225
gat tca gac ctt gtg cag gaa ctc aac act ggc tgc atg gtg gtg tcg 835
Asp Ser Asp Leu Val Gln Glu Leu Asn Thr Gly Cys Met Val Val Ser
230 235 240 245
caa aaa ctc atc gac gag cac ccc gca gtg gtt gga gaa tta gtg gat 883
Gln Lys Leu Ile Asp Glu His Pro Ala Val Val Gly Glu Leu Val Asp
250 255 260
gga gta gct cag gca atc gag ttt gaa cga tcc cac tcc cct gaa gaa 931
Gly Val Ala Gln Ala Ile Glu Phe Glu Arg Ser His Ser Pro Glu Glu
265 270 275
gtg cgc gaa gtt tat ttc aac tac ctc gaa gcc cat ggt cag agt gat 979
Val Arg Glu Val Tyr Phe Asn Tyr Leu Glu Ala His Gly Gln Ser Asp
280 285 290
aga ata tcc agc ttt aga tat tgg gag cat tcg ggc atc gca acc cga 1027
Arg Ile Ser Ser Phe Arg Tyr Trp Glu His Ser Gly Ile Ala Thr Arg
295 300 305
ggt gga gtg ctc agt gat agg gag ttc agc atg tgg tcc cac tgg att 1075
Gly Gly Val Leu Ser Asp Arg Glu Phe Ser Met Trp Ser His Trp Ile
310 315 320 325
gac cgc caa tac gac gtc ccc gat atc aat cca gca agt att tac acc 1123
Asp Arg Gln Tyr Asp Val Pro Asp Ile Asn Pro Ala Ser Ile Tyr Thr
330 335 340
aac caa ttc aac cca tac cga aaa gta aac ccc tcg cca taaaaggcaa 1172
Asn Gln Phe Asn Pro Tyr Arg Lys Val Asn Pro Ser Pro
345 350
gggggtcggc gttgaaattt 1192
<2l0>116
<211>354
<212>PRT
<213>谷氨酸棒状杆菌
<400>116
Val Arg Tyr Ile Ala Leu Ala Lys Val Thr Ser Met His Gln Arg Ser
1 5 10 15
Met Ser Phe Thr Phe Ile Arg Thr Phe Phe Val Leu Phe Gly Ile Thr
20 25 30
Leu Leu Val Ser Cys Val Pro Glu Pro Pro Asp Ser Tyr Thr Lys Glu
35 40 45
Ser Thr Val Leu Arg Tyr Gln Val Ser Asp Phe Asn Leu Asn Phe Val
50 55 60
Glu Leu Ala Val Ala Leu Gly Tyr Leu Asn Asn Ile Glu Leu Gln Val
65 70 75 80
Val Gly Ser Val Gln Gly Gly Val Glu Ser Ile Glu Ser Leu Lys Lys
85 90 95
Asp Asp Ile Asp Phe Ala Ala Val Pro Phe Ile Gly Leu Val Ala Gly
100 105 110
Glu Ile Ala Thr Gly Ala Pro Ile Lys Ala Val Ala Ala Ser Tyr Gly
115 120 125
Ile Ser His Asp Ser Ser Ser Ala Leu Leu Val Leu Lys Asp Ser Glu
130 135 140
Ile His Glu Val His Asp Leu Ile Gly Lys Thr Val Gly Ile Asn Thr
145 150 155 160
Leu Gly Ala Leu Gly Ser Ala Met Val Glu Arg His Leu Phe Asp Ala
165 170 175
Gly Leu Thr Glu Pro Glu Ile Val Ser Val Thr Gln Arg Ala Leu Pro
180 185 190
Gly Glu Tyr Leu Glu Gln Arg Leu Tyr Gln Gly Gln Val Asp Ala Ile
195 200 205
Trp Val Thr Asp Ser Ala Lys His Gln Ala Leu Glu Thr Gly Asp Phe
210 215 220
Arg Ile Leu Ala Glu Asp Ser Asp Leu Val Gln Glu Leu Asn Thr Gly
225 230 235 240
Cys Met Val Val Ser Gln Lys Leu Ile Asp Glu His Pro Ala Val Val
245 250 255
Gly Glu Leu Val Asp Gly Val Ala Gln Ala Ile Glu Phe Glu Arg Ser
260 265 270
His Ser Pro Glu Glu Val Arg Glu Val Tyr Phe Asn Tyr Leu Glu Ala
275 280 285
His Gly Gln Ser Asp Arg Ile Ser Ser Phe Arg Tyr Trp Glu His Ser
290 295 300
Gly Ile Ala Thr Arg Gly Gly Val Leu Ser Asp Arg Glu Phe Ser Met
305 310 315 320
Trp Ser His Trp Ile Asp Arg Gln Tyr Asp Val Pro Asp Ile Asn Pro
325 330 335
Ala Ser Ile Tyr Thr Asn Gln Phe Asn Pro Tyr Arg Lys Val Asn Pro
340 345 350
Ser Pro
<210>117
<211>1996
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1966)
<223>RXA01129
<400>117
caatataacg cactttaaca tatttccccc catctttttg ttctcaaatt tatttgtact 60
cgaatgcagg atttcgagac aatgaggcag tttttctttt atg gtt aat ctc atg 115
Met Val Asn Leu Met
1 5
tcg gtc gaa ctc gaa gaa atc cgc gat ttc tta gca gga ttt gaa cct 163
Ser Val Glu Leu Glu Glu Ile Arg Asp Phe Leu Ala Gly Phe Glu Pro
10 15 20
ttc gcg cag cta cca gcc gag gaa cta gat cag tta cct gga aaa atg 211
Phe Ala Gln Leu Pro Ala Glu Glu Leu Asp Gln Leu Pro Gly Lys Met
25 30 35
agc ttg cgc tat ttt cgg cgt ggg gaa gag atc atc cca att ggt gtg 259
Ser Leu Arg Tyr Phe Arg Arg Gly Glu Glu Ile Ile Pro Ile Gly Val
40 45 50
ccc aat cat tac atg ggg gta atc aga tcg ggt gcc att gat gtt ctt 307
Pro Asn His Tyr Met Gly Val Ile Arg Ser Gly Ala Ile Asp Val Leu
55 60 65
gat cag gag ggc gta ctg ctg gat cgc cgt gac gcg ggg cgc tcg ttt 355
Asp Gln Glu Gly Val Leu Leu Asp Arg Arg Asp Ala Gly Arg Ser Phe
70 75 80 85
ggc tat tcc acg atg ggg cca gaa cga aat tct cgg tac cgt atg gtt 403
Gly Tyr Ser Thr Met Gly Pro Glu Arg Asn Ser Arg Tyr Arg Met Val
90 95 100
gcc gtg gaa gat tcc ttg gtg ttg cgt ctg ggg cgt gat gat ttt gat 451
Ala Val Glu Asp Ser Leu Val Leu Arg Leu Gly Arg Asp Asp Phe Asp
105 110 115
gag tta gcc aag cgc aac cct gat ctg aac cgt tac tac tcc agc tgg 499
Glu Leu Ala Lys Arg Asn Pro Asp Leu Asn Arg Tyr Tyr Ser Ser Trp
120 125 130
tcg aag cgt atc cgc gcc gct gct gat caa ttg cgc caa gaa tct agt 547
Ser Lys Arg Ile Arg Ala Ala Ala Asp Gln Leu Arg Gln Glu Ser Ser
135 140 145
tcg aag gtg ctg cgc acg aag ttg ggg gag ttc aag atc gcc aac ccg 595
Ser Lys Val Leu Arg Thr Lys Leu Gly Glu Phe Lys Ile Ala Asn Pro
150 155 160 165
att tcc tgc agc ccg gac acc acg atc atg gat gct gcc atc aag atg 643
Ile Ser Cys Ser Pro Asp Thr Thr Ile Met Asp Ala Ala Ile Lys Met
170 175 180
gat gag ttc ggg gtg tct tcg ttg ctg gtg cag atc gat ggg gaa ctc 691
Asp Glu Phe Gly Val Ser Ser Leu Leu Val Gln Ile Asp Gly Glu Leu
185 190 195
aaa ggc atc atc acc gat cgc gat atg cgc agc agg gtg gtg gcg aag 739
Lys Gly Ile Ile Thr Asp Arg Asp Met Arg Ser Arg Val Val Ala Lys
200 205 210
gat ttg gat att cag ctg ccg gtc tct gag gtc atg acg gtg gat ccg 787
Asp Leu Asp Ile Gln Leu Pro Val Ser Glu Val Met Thr Val Asp Pro
215 220 225
cgt tgc gcg acc tcg cag ggg ttg gct ttt gag gcc atg ttg ttg atg 835
Arg Cys Ala Thr Ser Gln Gly Leu Ala Phe Glu Ala Met Leu Leu Met
230 235 240 245
tcg gag ctg cgc att cac cac ttg ccg att gtg gat gat ggc caa atc 883
Ser Glu Leu Arg Ile His His Leu Pro Ile Val Asp Asp Gly Gln Ile
250 255 260
tcc ggc atc gtc act gca gct gac atc atg cgc ttg ctg cgc cac gat 931
Ser Gly Ile Val Thr Ala Ala Asp Ile Met Arg Leu Leu Arg His Asp
265 270 275
ccg att tac ctc act gcg gat ctg tcg cga aaa aat acc gtg gag gaa 979
Pro Ile Tyr Leu Thr Ala Asp Leu Ser Arg Lys Asn Thr Val Glu Glu
280 285 290
ttg gcc aac acc ttc cag tcg gca gcg gag gtg gct tcg aga ttt att 1027
Leu Ala Asn Thr Phe Gln Ser Ala Ala Glu Val Ala Ser Arg Phe Ile
295 300 305
gat cgg gga gct tct gcg gaa gaa gtc agt agc ttg ctc acc gtg gct 1075
Asp Arg Gly Ala Ser Ala Glu Glu Val Ser Ser Leu Leu Thr Val Ala
310 315 320 325
gcg gat tct ttg gca aga agg ctc ctt gtg ctg gcg gag cgg aaa ttt 1123
Ala Asp Ser Leu Ala Arg Arg Leu Leu Val Leu Ala Glu Arg Lys Phe
330 335 340
ggt gca ccg cca gtt ccg tat tgc ttt gtg gtg gtg ggc tcg cag ggc 1171
Gly Ala Pro Pro Val Pro Tyr Cys Phe Val Val Val Gly Ser Gln Gly
345 350 355
agg aaa gaa atg gga ctg gcc tct gat caa gac aat gcc ctt gtt ctg 1219
Arg Lys Glu Met Gly Leu Ala Ser Asp Gln Asp Asn Ala Leu Val Leu
360 365 370
gac aat tcc tac aat gac cgc gag cat gga cag tat ttt gca gcg ctg 1267
Asp Asn Ser Tyr Asn Asp Arg Glu His Gly Gln Tyr Phe Ala Ala Leu
375 380 385
agt gaa ttc gtg tgc cag ggc ctc gac cgc gca gga cag gtg ctg tgt 1315
Ser Glu Phe Val Cys Gln Gly Leu Asp Arg Ala Gly Gln Val Leu Cys
390 395 400 405
ccg ggt gac atg atg gca tcc aat ccg gag tgg cgg aaa act gct gac 1363
Pro Gly Asp Met Met Ala Ser Asn Pro Glu Trp Arg Lys Thr Ala Asp
410 415 420
cag tgg atc tcc acc ttc cat tcc tgg att act gcg ccg gag ccg gat 1411
Gln Trp Ile Ser Thr Phe His Ser Trp Ile Thr Ala Pro Glu Pro Asp
425 430 435
gcg ttg ctg cat gcc cag aca ttc ttt gat ttc agg gga att tac ggc 1459
Ala Leu Leu His Ala Gln Thr Phe Phe Asp Phe Arg Gly Ile Tyr Gly
440 445 450
gac acc gag atg gct aag gat gtc cac caa aat gcg gtg aat atg gcc 1507
Asp Thr Glu Met Ala Lys Asp Val His Gln Asn Ala Val Asn Met Ala
455 460 465
agg ggt gcg cgt cgc atg cat gca cac ttg gct agt ctt gcg gcc cgg 1555
Arg Gly Ala Arg Arg Met His Ala His Leu Ala Ser Leu A1a Ala Arg
470 475 480 485
cgc gat cct ccg ctg ggg ttt ttc cgt ggt ctt gtg gtg gag cgt tct 1603
Arg Asp Pro Pro Leu Gly Phe Phe Arg Gly Leu Val Val Glu Arg Ser
490 495 500
ggt gaa tac ggc gcg acg atg gat att aag aag ggt ggc acc gcg ggc 1651
Gly Glu Tyr Gly Ala Thr Met Asp Ile Lys Lys Gly Gly Thr Ala Gly
505 510 515
atc gtg cag atg gcg agg ctg tat gcg ctg gcc acg ggt agt gat gcg 1699
Ile Val Gln Met Ala Arg Leu Tyr Ala Leu Ala Thr Gly Ser Asp Ala
520 525 530
att ggg act cgg gag cgg ttg att gct gcg tcg ggc cat ggt cag gtg 1747
Ile Gly Thr Arg Glu Arg Leu Ile Ala Ala Ser Gly His Gly Gln Val
535 540 545
tcg cgt aag ggt gcg cag gat ttg ttg gat gcc ttt gat ttc tta gcg 1795
Ser Arg Lys Gly Ala Gln Asp Leu Leu Asp Ala Phe Asp Phe Leu Ala
550 555 560 565
gcg atg gcg ttc cag cac cag gcg cgg ttg atc aag gtg ggg gag aag 1843
Ala Met Ala Phe Gln His Gln Ala Arg Leu Ile Lys Val Gly Glu Lys
570 575 580
ccg aac tat cac att gat ccc aag acg ttg ggc aag atg gat cgg gag 1891
Pro Asn Tyr His Ile Asp Pro Lys Thr Leu Gly Lys Met Asp Arg Glu
585 590 595
cat ttg cgc gat gca ttt tcc att att aaa gat atg cag tcg gcg tta 1939
His Leu Arg Asp Ala Phe Ser Ile Ile Lys Asp Met Gln Ser Ala Leu
600 605 610
gct act aaa tat ccg gtg agg aac atc tagtgttggg gcgtcgaaaa 1986
Ala Thr Lys Tyr Pro Val Arg Asn Ile
615 620
gcgaaaatac 1996
<210>118
<211>622
<212>PRT
<213>谷氨酸棒状杆菌
<400>118
Met Val Asn Leu Met Ser Val Glu Leu Glu Glu Ile Arg Asp Phe Leu
1 5 10 15
Ala Gly Phe Glu Pro Phe Ala Gln Leu Pro Ala Glu Glu Leu Asp Gln
20 25 30
Leu Pro Gly Lys Met Ser Leu Arg Tyr Phe Arg Arg Gly Glu Glu Ile
35 40 45
Ile Pro Ile Gly Val Pro Asn His Tyr Met Gly Val Ile Arg Ser Gly
50 55 60
Ala Ile Asp Val Leu Asp Gln Glu Gly Val Leu Leu Asp Arg Arg Asp
65 70 75 80
Ala Gly Arg Ser Phe Gly Tyr Ser Thr Met Gly Pro Glu Arg Asn Ser
85 90 95
Arg Tyr Arg Met Val Ala Val Glu Asp Ser Leu Val Leu Arg Leu Gly
100 105 110
Arg Asp Asp Phe Asp Glu Leu Ala Lys Arg Asn Pro Asp Leu Asn Arg
115 120 125
Tyr Tyr Ser Ser Trp Ser Lys Arg Ile Arg Ala Ala Ala Asp Gln Leu
130 135 140
Arg Gln Glu Ser Ser Ser Lys Val Leu Arg Thr Lys Leu Gly Glu Phe
145 150 155 160
Lys Ile Ala Asn Pro Ile Ser Cys Ser Pro Asp Thr Thr Ile Met Asp
165 170 175
Ala Ala Ile Lys Met Asp Glu Phe Gly Val Ser Ser Leu Leu Val Gln
180 185 190
Ile Asp Gly Glu Leu Lys Gly Ile Ile Thr Asp Arg Asp Met Arg Ser
195 200 205
Arg Val Val Ala Lys Asp Leu Asp Ile Gln Leu Pro Val Ser Glu Val
210 215 220
Met Thr Val Asp Pro Arg Cys Ala Thr Ser Gln Gly Leu Ala Phe Glu
225 230 235 240
Ala Met Leu Leu Met Ser Glu Leu Arg Ile His His Leu Pro Ile Val
245 250 255
Asp Asp Gly Gln Ile Ser Gly Ile Val Thr Ala Ala Asp Ile Met Arg
260 265 270
Leu Leu Arg His Asp Pro Ile Tyr Leu Thr Ala Asp Leu Ser Arg Lys
275 280 285
Asn Thr Val Glu Glu Leu Ala Asn Thr Phe Gln Ser Ala Ala Glu Val
290 295 300
Ala Ser Arg Phe Ile Asp Arg Gly Ala Ser Ala Glu Glu Val Ser Ser
305 310 315 320
Leu Leu Thr Val Ala Ala Asp Ser Leu Ala Arg Arg Leu Leu Val Leu
325 330 335
Ala Glu Arg Lys Phe Gly Ala Pro Pro Val Pro Tyr Cys Phe Val Val
340 345 350
Val Gly Ser Gln Gly Arg Lys Glu Met Gly Leu Ala Ser Asp Gln Asp
355 360 365
Asn Ala Leu Val Leu Asp Asn Ser Tyr Asn Asp Arg Glu His Gly Gln
370 375 380
Tyr Phe Ala Ala Leu Ser Glu Phe Val Cys Gln Gly Leu Asp Arg Ala
385 390 395 400
Gly Gln Val Leu Cys Pro Gly Asp Met Met Ala Ser Asn Pro Glu Trp
405 410 415
Arg Lys Thr Ala Asp Gln Trp Ile Ser Thr Phe His Ser Trp Ile Thr
420 425 430
Ala Pro Glu Pro Asp Ala Leu Leu His Ala Gln Thr Phe Phe Asp Phe
435 440 445
Arg Gly Ile Tyr Gly Asp Thr Glu Met Ala Lys Asp Val His Gln Asn
450 455 460
Ala Val Asn Met Ala Arg Gly Ala Arg Arg Met His Ala His Leu Ala
465 470 475 480
Ser Leu Ala Ala Arg Arg Asp Pro Pro Leu Gly Phe Phe Arg Gly Leu
485 490 495
Val Val Glu Arg Ser Gly Glu Tyr Gly Ala Thr Met Asp Ile Lys Lys
500 505 510
Gly Gly Thr Ala Gly Ile Val Gln Met Ala Arg Leu Tyr Ala Leu Ala
515 520 525
Thr Gly Ser Asp Ala Ile Gly Thr Arg Glu Arg Leu Ile Ala Ala Ser
530 535 540
Gly His Gly Gln Val Ser Arg Lys Gly Ala Gln Asp Leu Leu Asp Ala
545 550 555 560
Phe Asp Phe Leu Ala Ala Met Ala Phe Gln His Gln Ala Arg Leu Ile
565 570 575
Lys Val Gly Glu Lys Pro Asn Tyr His Ile Asp Pro Lys Thr Leu Gly
580 585 590
Lys Met Asp Arg Glu His Leu Arg Asp Ala Phe Ser Ile Ile Lys Asp
595 600 605
Met Gln Ser Ala Leu Ala Thr Lys Tyr Pro Val Arg Asn Ile
610 615 620
<210>119
<211>745
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(715)
<223>RXA01173
<400>119
agatctgttt ctatgtatta aagatcacac cgagtggtgg aatttcctca agtgatttac 60
ccacaatgga ctttgttgat acccaattcg agaaaggcca atg cac gtg agc act 115
Met His Val Ser Thr
1 5
ctt cca aac aag aaa ctg cgt act cgc att ttc gca ggc acc gct gcc 163
Leu Pro Asn Lys Lys Leu Arg Thr Arg Ile Phe Ala Gly Thr Ala Ala
10 15 20
gtc gca ctg tca ctt ggt gtt gcg tct tgc tca aac gca gaa gat gct 211
Val Ala Leu Ser Leu Gly Val Ala Ser Cys Ser Asn Ala Glu Asp Ala
25 30 35
gtg gat agc gca aca gat gct gcc aac tct gca acc tcc gcc gcg gga 259
Val Asp Ser Ala Thr Asp Ala Ala Asn Ser Ala Thr Ser Ala Ala Gly
40 45 50
tct gca att aac gat gcc acc ggc act tcc agc gca tcc acc aca gag 307
Ser Ala Ile Asn Asp Ala Thr Gly Thr Ser Ser Ala Ser Thr Thr Glu
55 60 65
cct tcc gga acc tct gga tcc gac tcc ggg tct gac tct gct gga gga 355
Pro Ser Gly Thr Ser Gly Ser Asp Ser Gly Ser Asp Ser Ala Gly Gly
70 75 80 85
gac acc act gaa gta gaa agc gcc gat ggg tcc acc atc agc atc cca 403
Asp Thr Thr Glu Val Glu Ser Ala Asp Gly Ser Thr Ile Ser Ile Pro
90 95 100
act gcc gtc gtc acc gct gca aat gct gca gga ttc agt acc ccg gaa 451
Thr Ala Val Val Thr Ala Ala Asn Ala Ala Gly Phe Ser Thr Pro Glu
105 110 115
tcc gtg gaa gaa ggc ccg aat ggt gag tca ttg gtg acg ttc cct gaa 499
Ser Val Glu Glu Gly Pro Asn Gly Glu Ser Leu Val Thr Phe Pro Glu
120 125 130
ggc tac att gtt aac tct gca gaa ggt ggt gca caa gca ctg gtc ggc 547
Gly Tyr Ile Val Asn Ser Ala Glu Gly Gly Ala Gln Ala Leu Val Gly
135 140 145
atg atc ggt gaa acc tgg atc ggc gaa ggc gga cta tcc gcg gca gtg 595
Met Ile Gly Glu Thr Trp Ile Gly Glu Gly Gly Leu Ser Ala Ala Val
150 155 160 165
ggt ctc cca act ggg cct gaa gaa gca aca aca aat ggt tgg act caa 643
Gly Leu Pro Thr Gly Pro Glu Glu Ala Thr Thr Asn Gly Trp Thr Gln
170 175 180
cag ttc aca tct gga gta att agc tgg ctt gat gat gga tca gga cag 691
Gln Phe Thr Ser Gly Val Ile Ser Trp Leu Asp Asp Gly Ser Gly Gln
185 190 195
ttc gca gct tct gtt gaa cct gct taagggaatc tcacctggcc tcctttggca 745
Phe Ala Ala Ser Val Glu Pro Ala
200 205
<210>120
<211>205
<212>PRT
<213>谷氨酸棒状杆菌
<400>120
Met His Val Ser Thr Leu Pro Asn Lys Lys Leu Arg Thr Arg Ile Phe
1 5 10 15
Ala Gly Thr Ala Ala Val Ala Leu Ser Leu Gly Val Ala Ser Cys Ser
20 25 30
Asn Ala Glu Asp Ala Val Asp Ser Ala Thr Asp Ala Ala Asn Ser Ala
35 40 45
Thr Ser Ala Ala Gly Ser Ala Ile Asn Asp Ala Thr Gly Thr Ser Ser
50 55 60
Ala Ser Thr Thr Glu Pro Ser Gly Thr Ser Gly Ser Asp Ser Gly Ser
65 70 75 80
Asp Ser Ala Gly Gly Asp Thr Thr Glu Val Glu Ser Ala Asp Gly Ser
85 90 95
Thr Ile Ser Ile Pro Thr Ala Val Val Thr Ala Ala Asn Ala Ala Gly
100 105 110
Phe Ser Thr Pro Glu Ser Val Glu Glu Gly Pro Asn Gly Glu Ser Leu
115 120 125
Val Thr Phe Pro Glu Gly Tyr Ile Val Asn Ser Ala Glu Gly Gly Ala
130 135 140
Gln Ala Leu Val Gly Met Ile Gly Glu Thr Trp Ile Gly Glu Gly Gly
145 150 155 160
Leu Ser Ala Ala Val Gly Leu Pro Thr Gly Pro Glu Glu Ala Thr Thr
165 170 175
Asn Gly Trp Thr Gln Gln Phe Thr Ser Gly Val Ile Ser Trp Leu Asp
180 185 190
Asp Gly Ser Gly Gln Phe Ala Ala Ser Val Glu Pro Ala
195 200 205
<210>121
<211>880
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(850)
<223>RXA01174
<400>121
atatcgcaac cgctgttaat gaagaggaag aggctcgcga tatcgcgaag ctgtgccagg 60
agatggtgga attagctcgg aatattgaga agttgaggta atg agc aac atg cag 115
Met Ser Asn Met Gln
1 5
gga aac gat tcc aag aaa tct agc ggc gcg agt cgt gcg gag agc cca 163
Gly Asn Asp Ser Lys Lys Ser Ser Gly Ala Ser Arg Ala Glu Ser Pro
10 15 20
ctg atc aag ttc cgg aca ttg att atc gtc atc ttt gtc atc ttg atc 211
Leu Ile Lys Phe Arg Thr Leu Ile Ile Val Ile Phe Val Ile Leu Ile
25 30 35
gtt ggt ttg gca tct att gca gtg gga cct gtc gtg tac cag ctc atc 259
Val Gly Leu Ala Ser Ile Ala Val Gly Pro Val Val Tyr Gln Leu Ile
40 45 50
atg gga cct ggt gtg aaa acc gaa gga atc cag gct gat ggc gca gca 307
Met Gly Pro Gly Val Lys Thr Glu Gly Ile Gln Ala Asp Gly Ala Ala
55 60 65
cct gcg tcc acc gac atg aac ggc acc tgg gat gtt gcc cca ggg agt 355
Pro Ala Ser Thr Asp Met Asn Gly Thr Trp Asp Val Ala Pro Gly Ser
70 75 80 85
att cca aac acc acc tca gct gga ttc acc ttc gct gag atc ctg cca 403
Ile Pro Asn Thr Thr Ser Ala Gly Phe Thr Phe Ala Glu Ile Leu Pro
90 95 100
ggc gaa gaa aag atc acc tcc ggc tca acc act ggt gtc act ggc gaa 451
Gly Glu Glu Lys Ile Thr Ser Gly Ser Thr Thr Gly Val Thr Gly Glu
105 110 115
gtg gtc atc gag gat aac tcc ctg atc tct ggt ctg att acc gtc aac 499
Val Val Ile Glu Asp Asn Ser Leu Ile Ser Gly Leu Ile Thr Val Asn
120 125 130
atg act cac atc acc acc gat cag gaa aag cgc gac atc aac gtg cgc 547
Met Thr His Ile Thr Thr Asp Gln Glu Lys Arg Asp Ile Asn Val Arg
135 140 145
act aag ctc ttc cac acc gat cag tac cca gaa gca acc ttt gag gtt 595
Thr Lys Leu Phe His Thr Asp Gln Tyr Pro Glu Ala Thr Phe Glu Val
150 155 160 165
acc gat tcc gtt gat ctt tct gcg ctc cca gac acc gga tcc att gct 643
Thr Asp Ser Val Asp Leu Ser Ala Leu Pro Asp Thr Gly Ser Ile Ala
170 175 180
cag gtt gtc atc cca ggc gag ttg acc atc cac ggt gaa acc aag gct 691
Gln Val Val Ile Pro Gly Glu Leu Thr Ile His Gly Glu Thr Lys Ala
185 190 195
gtg gag cct acc ttt gat gta ctt cgt act ggt gac caa gtt atc gtg 739
Val Glu Pro Thr Phe Asp Val Leu Arg Thr Gly Asp Gln Val Ile Val
200 205 210
gct tcc gat atc gaa atc aac cgc ctc gac ttc ggt gta gaa acc cca 787
Ala Ser Asp Ile Glu Ile Asn Arg Leu Asp Phe Gly Val Glu Thr Pro
215 220 225
gag ttc atc gcc gca aag atc aat gag acc ggc gag atc aac gtc cga 835
Glu Phe Ile Ala Ala Lys Ile Asn Glu Thr Gly Glu Ile Asn Val Arg
230 235 240 245
atc gta ttg gag aaa taaaccatga tggcatcacg gatgatccca 880
Ile Val Leu Glu Lys
250
<210>122
<211>250
<212>PRT
<213>谷氨酸棒状杆菌
<400>122
Met Ser Asn Met Gln Gly Asn Asp Ser Lys Lys Ser Ser Gly Ala Ser
1 5 10 15
Arg Ala Glu Ser Pro Leu Ile Lys Phe Arg Thr Leu Ile Ile Val Ile
20 25 30
Phe Val Ile Leu Ile Val Gly Leu Ala Ser Ile Ala Val Gly Pro Val
35 40 45
Val Tyr Gln Leu Ile Met Gly Pro Gly Val Lys Thr Glu Gly Ile Gln
50 55 60
Ala Asp Gly Ala Ala Pro Ala Ser Thr Asp Met Asn Gly Thr Trp Asp
65 70 75 80
Val Ala Pro Gly Ser Ile Pro Asn Thr Thr Ser Ala Gly Phe Thr Phe
85 90 95
Ala Glu Ile Leu Pro Gly Glu Glu Lys Ile Thr Ser Gly Ser Thr Thr
100 105 110
Gly Val Thr Gly Glu Val Val Ile Glu Asp Asn Ser Leu Ile Ser Gly
115 120 125
Leu Ile Thr Val Asn Met Thr His Ile Thr Thr Asp Gln Glu Lys Arg
130 135 140
Asp Ile Asn Val Arg Thr Lys Leu Phe His Thr Asp Gln Tyr Pro Glu
145 150 155 160
Ala Thr Phe Glu Val Thr Asp Ser Val Asp Leu Ser Ala Leu Pro Asp
165 170 175
Thr Gly Ser Ile Ala Gln Val Val Ile Pro Gly Glu Leu Thr Ile His
180 185 190
Gly Glu Thr Lys Ala Val Glu Pro Thr Phe Asp Val Leu Arg Thr Gly
195 200 205
Asp Gln Val Ile Val Ala Ser Asp Ile Glu Ile Asn Arg Leu Asp Phe
210 215 220
Gly Val Glu Thr Pro Glu Phe Ile Ala Ala Lys Ile Asn Glu Thr Gly
225 230 235 240
Glu Ile Asn Val Arg Ile Val Leu Glu Lys
245 250
<210>123
<211>1675
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1645)
<223>RXA01184
<400>123
atcttggttg ggtgcgtccg aaggccatag cgaaggccaa aatcagaacg aggatcgggc 60
tgagtaaatg actaaaacac ttctgaaact acagctgacg ttg tgg gga aga act 115
Leu Trp Gly Arg Thr
1 5
att aaa gga aat aaa gcg gca ata gtg atg atg tcg ctg ata tct ttt 163
Ile Lys Gly Asn Lys Ala Ala Ile Val Met Met Ser Leu Ile Ser Phe
10 15 20
tac gcc ttg atg ggt ttg ttt ggt ttc ggc atc ttt ttg gct ctt ggg 211
Tyr Ala Leu Met Gly Leu Phe Gly Phe Gly Ile Phe Leu Ala Leu Gly
25 30 35
ctt ggg caa gac cac atg ggc atc ctt gct ggt gtt gtc tcg gtc ggc 259
Leu Gly Gln Asp His Met Gly Ile Leu Ala Gly Val Val Ser Val Gly
40 45 50
atg gtg gct tat tgc att gct gcg ttt atg tgg ccg tca ggt gag ggg 307
Met Val Ala Tyr Cys Ile Ala Ala Phe Met Trp Pro Ser Gly Glu Gly
55 60 65
cag ctg gat ccg act gca ttt tcc acc atg ccg cta tcg gcg aag caa 355
Gln Leu Asp Pro Thr Ala Phe Ser Thr Met Pro Leu Ser Ala Lys Gln
70 75 80 85
tta ttg cct gga ttc gct atc ggt acg ctc ctg cag tca cgg ggg att 403
Leu Leu Pro Gly Phe Ala Ile Gly Thr Leu Leu Gln Ser Arg Gly Ile
90 95 100
att gcg gtg att tgt acg gtg gcg aca tcc att att gct gcg gtg ttt 451
Ile Ala Val Ile Cys Thr Val Ala Thr Ser Ile Ile Ala Ala Val Phe
105 110 115
tta cct gtt ggt tcg tgg ccg atg atc gtg ttc atg atg gcg gta tcg 499
Leu Pro Val Gly Ser Trp Pro Met Ile Val Phe Met Met Ala Val Ser
120 125 130
ctg gtt act acg ttg ctt ttg ggt gag ttg ctt ggg gcg ttg acc tcg 547
Leu Val Thr Thr Leu Leu Leu Gly Glu Leu Leu Gly Ala Leu Thr Ser
135 140 145
ggt tct tct tct agg gtg agt aat gat cgc cgt aca gtt ctt act tcc 595
Gly Ser Ser Ser Arg Val Ser Asn Asp Arg Arg Thr Val Leu Thr Ser
150 155 160 165
gtt gtc ttc atg gtg ttt gtt gtt ggc tat aac atg ctg att ggc gcg 643
Val Val Phe Met Val Phe Val Val Gly Tyr Asn Met Leu Ile Gly Ala
170 175 180
gat gga atg agt cga atc gat gcc att ggg gct tat aca aag tgg aca 691
Asp Gly Met Ser Arg Ile Asp Ala Ile Gly Ala Tyr Thr Lys Trp Thr
185 190 195
cca ttt ggg gcg ggt gca ggt gct att gag gct ttt gct gtt ggt ttg 739
Pro Phe Gly Ala Gly Ala Gly Ala Ile Glu Ala Phe Ala Val Gly Leu
200 205 210
tgg ggt gag gca ggc ctg tta aca ttg ctg gct ttt gtt tat gtt gct 787
Trp Gly Glu Ala Gly Leu Leu Thr Leu Leu Ala Phe Val Tyr Val Ala
215 220 225
gct ggt ttc tgg tta tgg tca cag ctg att aat cgc gcg ctc act gcg 835
Ala Gly Phe Trp Leu Trp Ser Gln Leu Ile Asn Arg Ala Leu Thr Ala
230 235 240 245
ccg ctg gac caa ggt ggg caa ggg caa agc gct aaa gac agc gcc ggt 883
Pro Leu Asp Gln Gly Gly Gln Gly Gln Ser Ala Lys Asp Ser Ala Gly
250 255 260
gaa ggc aag aag gtt ctc ttt ctg cca ggt att cct tgg tca gtt gga 931
Glu Gly Lys Lys Val Leu Phe Leu Pro Gly Ile Pro Trp Ser Val Gly
265 270 275
ggt gcc att ttc tcg agg tcg ctg cgt tat atg ttc agg gac tcc agg 979
Gly Ala Ile Phe Ser Arg Ser Leu Arg Tyr Met Phe Arg Asp Ser Arg
280 285 290
ttg ttg gga tca atg att gtg ttc ccg ctt ctt ggc gtg ctg ttt att 1027
Leu Leu Gly Ser Met Ile Val Phe Pro Leu Leu Gly Val Leu Phe Ile
295 300 305
ttc cag agc ttc acc gtt gag ttt ttc atg atc tat gtt ggg ttg atc 1075
Phe Gln Ser Phe Thr Val Glu Phe Phe Met Ile Tyr Val Gly Leu Ile
310 315 320 325
atg atg gca gtg ttc gca gga tcc gtt gct acc aat gat ttt ggc tat 1123
Met Met Ala Val Phe Ala Gly Ser Val Ala Thr Asn Asp Phe Gly Tyr
330 335 340
gac ggc cct tcg ttg tgg cta aat atc gtt gct ggt gtc aaa gcc cga 1171
Asp Gly Pro Ser Leu Trp Leu Asn Ile Val Ala Gly Val Lys Ala Arg
345 350 355
acg ttg ttg atg ccc agg cac tgg gca tca atg ct9 ccg gga agt gtg 1219
Thr Leu Leu Met Pro Arg His Trp Ala Ser Met Leu Pro Gly Ser Val
360 365 370
tca att gtg gtg ttt atg atc atc acc att gtg ctc gcg gag aat aag 1267
Ser Ile Val Val Phe Met Ile Ile Thr Ile Val Leu Ala Glu Asn Lys
375 380 385
acc acc gct gtg ctg atc tgt ttt att ggc ctg ggg atc ttt atc tcc 1315
Thr Thr Ala Val Leu Ile Cys Phe Ile Gly Leu Gly Ile Phe Ile Ser
390 395 400 405
agt gcg gct gta gcg ttg ttg gtc acc aca ttt aat ccg tat ccg act 1363
Ser Ala Ala Val Ala Leu Leu Val Thr Thr Phe Asn Pro Tyr Pro Thr
410 415 420
tct aag cca ggc acg agt cct tgg ggc gat cga agt ggc tat tcc ggt 1411
Ser Lys Pro Gly Thr Ser Pro Trp Gly Asp Arg Ser Gly Tyr Ser Gly
425 430 435
gct gcg ttc gtg gga gca ttt gcc gca ctg tta ctg gga tgg atc ccg 1459
Ala Ala Phe Val Gly Ala Phe Ala Ala Leu Leu Leu Gly Trp Ile Pro
440 445 450
acg att cct act atc gca ttg ggt atc ttt ggt ctg gtt acc gat cag 1507
Thr Ile Pro Thr Ile Ala Leu Gly Ile Phe Gly Leu Val Thr Asp Gln
455 460 465
atg tgg atg atc atc ctc gcg gag gtg ctg gcc att att ctc cca gta 1555
Met Trp Met Ile Ile Leu Ala Glu Val Leu Ala Ile Ile Leu Pro Val
470 475 480 485
gct gtg tac atc ggc gtt gct aag gtg tgt att cgc aag gtg gag aag 1603
Ala Val Tyr Ile Gly Val Ala Lys Val Cys Ile Arg Lys Val Glu Lys
490 495 500
gat ctt ccg gag atc ttc gac aag gtg aaa act cac gtg aaa 1645
Asp Leu Pro Glu Ile Phe Asp Lys Val Lys Thr His Val Lys
505 510 515
tagaaaaatg cgggtgcttc gagaaaatct 1675
<210>124
<211>515
<212>PRT
<213>谷氨酸棒状杆菌
<400>124
Leu Trp Gly Arg Thr Ile Lys Gly Asn Lys Ala Ala Ile Val Met Met
1 5 10 15
Ser Leu Ile Ser Phe Tyr Ala Leu Met Gly Leu Phe Gly Phe Gly Ile
20 25 30
Phe Leu Ala Leu Gly Leu Gly Gln Asp His Met Gly Ile Leu Ala Gly
35 40 45
Val Val Ser Val Gly Met Val Ala Tyr Cys Ile Ala Ala Phe Met Trp
50 55 60
Pro Ser Gly Glu Gly Gln Leu Asp Pro Thr Ala Phe Ser Thr Met Pro
65 70 75 80
Leu Ser Ala Lys Gln Leu Leu Pro Gly Phe Ala Ile Gly Thr Leu Leu
85 90 95
Gln Ser Arg Gly Ile Ile Ala Val Ile Cys Thr Val Ala Thr Ser Ile
100 105 110
Ile Ala Ala Val Phe Leu Pro Val Gly Ser Trp Pro Met Ile Val Phe
115 120 125
Met Met Ala Val Ser Leu Val Thr Thr Leu Leu Leu Gly Glu Leu Leu
130 135 140
Gly Ala Leu Thr Ser Gly Ser Ser Ser Arg Val Ser Asn Asp Arg Arg
145 150 155 160
Thr Val Leu Thr Ser Val Val Phe Met Val Phe Val Val Gly Tyr Asn
165 170 175
Met Leu Ile Gly Ala Asp Gly Met Ser Arg Ile Asp Ala Ile Gly Ala
180 185 190
Tyr Thr Lys Trp Thr Pro Phe Gly Ala Gly Ala Gly Ala Ile Glu Ala
195 200 205
Phe Ala Val Gly Leu Trp Gly Glu Ala Gly Leu Leu Thr Leu Leu Ala
210 215 220
Phe Val Tyr Val Ala Ala Gly Phe Trp Leu Trp Ser Gln Leu Ile Asn
225 230 235 240
Arg Ala Leu Thr Ala Pro Leu Asp Gln Gly Gly Gln Gly Gln Ser Ala
245 250 255
Lys Asp Ser Ala Gly Glu Gly Lys Lys Val Leu Phe Leu Pro Gly Ile
260 265 270
Pro Trp Ser Val Gly Gly Ala Ile Phe Ser Arg Ser Leu Arg Tyr Met
275 280 285
Phe Arg Asp Ser Arg Leu Leu Gly Ser Met Ile Val Phe Pro Leu Leu
290 295 300
Gly Val Leu Phe Ile Phe Gln Ser Phe Thr Val Glu Phe Phe Met Ile
305 310 315 320
Tyr Val Gly Leu Ile Met Met Ala Val Phe Ala Gly Ser Val Ala Thr
325 330 335
Asn Asp Phe Gly Tyr Asp Gly Pro Ser Leu Trp Leu Asn Ile Val Ala
340 345 350
Gly Val Lys Ala Arg Thr Leu Leu Met Pro Arg His Trp Ala Ser Met
355 360 365
Leu Pro Gly Ser Val Ser Ile Val Val Phe Met Ile Ile Thr Ile Val
370 375 380
Leu Ala Glu Asn Lys Thr Thr Ala Val Leu Ile Cys Phe Ile Gly Leu
385 390 395 400
Gly Ile Phe Ile Ser Ser Ala Ala Val Ala Leu Leu Val Thr Thr Phe
405 410 415
Asn Pro Tyr Pro Thr Ser Lys Pro Gly Thr Ser Pro Trp Gly Asp Arg
420 425 430
Ser Gly Tyr Ser Gly Ala Ala Phe Val Gly Ala Phe Ala Ala Leu Leu
435 440 445
Leu Gly Trp Ile Pro Thr Ile Pro Thr Ile Ala Leu Gly Ile Phe Gly
450 455 460
Leu Val Thr Asp Gln Met Trp Met Ile Ile Leu Ala Glu Val Leu Ala
465 470 475 480
Ile Ile Leu Pro Val Ala Val Tyr Ile Gly Val Ala Lys Val Cys Ile
485 490 495
Arg Lys Val Glu Lys Asp Leu Pro Glu Ile Phe Asp Lys Val Lys Thr
500 505 510
His Val Lys
515
<210>125
<211>820
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(790)
<223>RXA01196
<400>125
gggcggagca ttcattgaat ccacgtgatt tcaaccgctt tcgagccagc aaagacaccc 60
gcaaaaaccg ctaagtagac gtcattgtta tggtgggtgc atg cgt tta gtc atc 115
Met Arg Leu Val Ile
1 5
gcc cgt tgc tca gtt gat tat gtt ggc cgt ttg gaa gct cat ctt ccg 163
Ala Arg Cys Ser Val Asp Tyr Val Gly Arg Leu Glu Ala His Leu Pro
10 15 20
tcc gcc gat cgc ctt ttg atg gtt aag gcg gat ggt tct gta tcc atc 211
Ser Ala Asp Arg Leu Leu Met Val Lys Ala Asp Gly Ser Val Ser Ile
25 30 35
cat gcc gat gac cgt gcc tat aag cca ctg aac tgg atg aca cct cca 259
His Ala Asp Asp Arg Ala Tyr Lys Pro Leu Asn Trp Met Thr Pro Pro
40 45 50
tgt tct tta gtt gaa act ccc atc act gat gaa gat ggt gaa gca aca 307
Cys Ser Leu Val Glu Thr Pro Ile Thr Asp Glu Asp Gly Glu Ala Thr
55 60 65
ggg gaa agc ctg tgg gtg gtg gaa aac aaa aag ggc gag cag ctt cga 355
Gly Glu Ser Leu Trp Val Val Glu Asn Lys Lys Gly Glu Gln Leu Arg
70 75 80 85
att act gtg gaa gaa att cat tcg gaa caa aac ttc gat cta ggc caa 403
Ile Thr Val Glu Glu Ile His Ser Glu Gln Asn Phe Asp Leu Gly Gln
90 95 100
gac cca ggt ttg gtg aaa gac gga gtg gaa gat cat ctc caa gag ctt 451
Asp Pro Gly Leu Val Lys Asp Gly Val Glu Asp His Leu Gln Glu Leu
105 110 115
ctt gca gag cac atc act acg ttg ggt gat ggg tac aca ttg att cgt 499
Leu Ala Glu His Ile Thr Thr Leu Gly Asp Gly Tyr Thr Leu Ile Arg
120 125 130
cgg gag tat cca aca gct att ggg cct gtc gat att ttg tgt cgc aac 547
Arg Glu Tyr Pro Thr Ala Ile Gly Pro Val Asp Ile Leu Cys Arg Asn
135 140 145
tct gac ggc gag act gtc gct gtg gag atc aag cgt cgt ggt ggc atc 595
Ser Asp Gly Glu Thr Val Ala Val Glu Ile Lys Arg Arg Gly Gly Ile
150 155 160 165
gac ggc gtt gag cag ttg acc agg tat ttg gaa ttg ctc aac cgt gat 643
Asp Gly Val Glu Gln Leu Thr Arg Tyr Leu Glu Leu Leu Asn Arg Asp
170 175 180
gaa ttg ctc aag cct gtt cat gga gtg ttt gca gcc cag gag att aag 691
Glu Leu Leu Lys Pro Val His Gly Val Phe Ala Ala Gln Glu Ile Lys
185 190 195
cct cag gca aag act ctc gcg gag gat cgt ggc atc aag tgc gtg acg 739
Pro Gln Ala Lys Thr Leu Ala Glu Asp Arg Gly Ile Lys Cys Val Thr
200 205 210
ttg gat tat caa gcg ctt cgt ggc att gag tcc aat gag ctg aca ttg 787
Leu Asp Tyr Gln Ala Leu Arg Gly Ile Glu Ser Asn Glu Leu Thr Leu
215 220 225
ttc taagaacatg ggccgaaaaa atagaagaac 820
Phe
230
<210>126
<211>230
<212>PRT
<213>谷氨酸棒状杆菌
<400>126
Met Arg Leu Val Ile Ala Arg Cys Ser Val Asp Tyr Val Gly Arg Leu
1 5 10 15
Glu Ala His Leu Pro Ser Ala Asp Arg Leu Leu Met Val Lys Ala Asp
20 25 30
Gly Ser Val Ser Ile His Ala Asp Asp Arg Ala Tyr Lys Pro Leu Asn
35 40 45
Trp Met Thr Pro Pro Cys Ser Leu Val Glu Thr Pro Ile Thr Asp Glu
50 55 60
Asp Gly Glu Ala Thr Gly Glu Ser Leu Trp Val Val Glu Asn Lys Lys
65 70 75 80
Gly Glu Gln Leu Arg Ile Thr Val Glu Glu Ile His Ser Glu Gln Asn
85 90 95
Phe Asp Leu Gly Gln Asp Pro Gly Leu Val Lys Asp Gly Val Glu Asp
100 105 110
His Leu Gln Glu Leu Leu Ala Glu His Ile Thr Thr Leu Gly Asp Gly
115 120 125
Tyr Thr Leu Ile Arg Arg Glu Tyr Pro Thr Ala Ile Gly Pro Val Asp
130 135 140
Ile Leu Cys Arg Asn Ser Asp Gly Glu Thr Val Ala Val Glu Ile Lys
145 150 155 160
Arg Arg Gly Gly Ile Asp Gly Val Glu Gln Leu Thr Arg Tyr Leu Glu
165 170 175
Leu Leu Asn Arg Asp Glu Leu Leu Lys Pro Val His Gly Val Phe Ala
180 185 190
Ala Gln Glu Ile Lys Pro Gln Ala Lys Thr Leu Ala Glu Asp Arg Gly
195 200 205
Ile Lys Cys Val Thr Leu Asp Tyr Gln Ala Leu Arg Gly Ile Glu Ser
210 215 220
Asn Glu Leu Thr Leu Phe
225 230
<210>127
<211>1300
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1270)
<223>RXA01263
<400>127
gggaggtcgt gcggaaaaag gtgtaggtgt acttctaaaa gttaaaccta tgcgttggat 60
cggagatctt tcctattcgc tttatctata gcattggcca ttg atc gtt gta ggg 115
Leu Ile Val Val Gly
1 5
att tat tat ttc aat gga gaa atg cac ttt tgg caa ggt ctc cta att 163
Ile Tyr Tyr Phe Asn Gly Glu Met His Phe Trp Gln Gly Leu Leu Ile
10 15 20
atc ggg ttc tct ttc atc cct gct tgg cta agt tat cat ttc att gag 211
Ile Gly Phe Ser Phe Ile Pro Ala Trp Leu Ser Tyr His Phe Ile Glu
25 30 35
aat cca atg cgt aat tgg tca gca atc aag gct gac gca aga aaa gca 259
Asn Pro Met Arg Asn Trp Ser Ala Ile Lys Ala Asp Ala Arg Lys Ala
40 45 50
gtg gcc ttt gga gtt att ttg atg acg gta tcg gcg act gct ggg atc 307
Val Ala Phe Gly Val Ile Leu Met Thr Val Ser Ala Thr Ala Gly Ile
55 60 65
ctc ctc ttt tta tct cca aat aga agc caa gct gcg cca ccc caa ttt 355
Leu Leu Phe Leu Ser Pro Asn Arg Ser Gln Ala Ala Pro Pro Gln Phe
70 75 80 85
agt ttg acc ccg tat gat cca acc gct gta aat aag gag tcg gaa aaa 403
Ser Leu Thr Pro Tyr Asp Pro Thr Ala Val Asn Lys Glu Ser Glu Lys
90 95 100
gaa gca gca aag aat ctg ttt ggc gct gag gcg ttg aca gtg gat ccg 451
Glu Ala Ala Lys Asn Leu Phe Gly Ala Glu Ala Leu Thr Val Asp Pro
105 110 115
gat gcc ggt gag gtt gtt gat cga gta gat aat ttt tat ccg acg act 499
Asp Ala Gly Glu Val Val Asp Arg Val Asp Asn Phe Tyr Pro Thr Thr
120 125 130
gct aag gca aaa cga gat tac cca agt aac tat gca gcg ggt tgt cac 547
Ala Lys Ala Lys Arg Asp Tyr Pro Ser Asn Tyr Ala Ala Gly Cys His
135 140 145
caa gaa gtc aat gag act agt cct gag tca tgt gtt tat ggt gat aaa 595
Gln Glu Val Asn Glu Thr Ser Pro Glu Ser Cys Val Tyr Gly Asp Lys
150 155 160 165
aat tct gat ttt tct gta gca ctt gtc ggt gac tcc cac gct ggt cat 643
Asn Ser Asp Phe Ser Val Ala Leu Val Gly Asp Ser His Ala Gly His
170 175 180
tgg ctt cct gcc ttg gaa cca att gct gaa gca cag ggg tgg aga ttg 691
Trp Leu Pro Ala Leu Glu Pro Ile Ala Glu Ala Gln Gly Trp Arg Leu
185 190 195
gaa gtt tat aca aag tca cag tgt cca ctc ata agc act gcg atc aaa 739
Glu Val Tyr Thr Lys Ser Gln Cys Pro Leu Ile Ser Thr Ala Ile Lys
200 205 210
ctt ggt gaa act ttt tat gca gaa tgc tat gag tgg aat gaa aaa tta 787
Leu Gly Glu Thr Phe Tyr Ala Glu Cys Tyr Glu Trp Asn Glu Lys Leu
215 220 225
ctt gct aag cta act gga cct tct gca cca aat cat gtg att gta agt 835
Leu Ala Lys Leu Thr Gly Pro Ser Ala Pro Asn His Val Ile Val Ser
230 235 240 245
agc caa cgt tac gct tct gca aat ccg tta atc gat agt gtc gcg acg 883
Ser Gln Arg Tyr Ala Ser Ala Asn Pro Leu Ile Asp Ser Val Ala Thr
250 255 260
gga acc gtt tcc gaa gga tat gaa atg gca tgg aat tca tta aaa gat 931
Gly Thr Val Ser Glu Gly Tyr Glu Met Ala Trp Asn Ser Leu Lys Asp
265 270 275
gca ggt gtt tct att tct gta ctt ctt gat act cct cgg ccg caa att 979
Ala Gly Val Ser Ile Ser Val Leu Leu Asp Thr Pro Arg Pro Gln Ile
280 285 290
gat atc cca gaa tgt gta gca tca aac cgc gat aat ctc tca gaa tgt 1027
Asp Ile Pro Glu Cys Val Ala Ser Asn Arg Asp Asn Leu Ser Glu Cys
295 300 305
tca gtt cac cgg agc gtt gcg ctt ggg act gaa gct cat cct cag caa 1075
Ser Val His Arg Ser Val Ala Leu Gly Thr Glu Ala His Pro Gln Gln
310 315 320 325
aaa act gca gct caa aat ata gac gtg cct gta ttg gat ttg agt aat 1123
Lys Thr Ala Ala Gln Asn Ile Asp Val Pro Val Leu Asp Leu Ser Asn
330 335 340
tgg att tgt ccg gaa gaa tat tgc tcc gct gtt atc gga aat gtt ttg 1171
Trp Ile Cys Pro Glu Glu Tyr Cys Ser Ala Val Ile Gly Asn Val Leu
345 350 355
gta tac agg gat tca cat cat ttg acc gct acg tat gct cgt agt ctc 1219
Val Tyr Arg Asp Ser His His Leu Thr Ala Thr Tyr Ala Arg Ser Leu
360 365 370
tct agc gca tta tgg aat gag ttg gtt gcc tca aat ggt gag cct ttt 1267
Ser Ser Ala Leu Trp Asn Glu Leu Val Ala Ser Asn Gly Glu Pro Phe
375 380 385
aag taagaggtagttgttcaagt agcgctttgt 1300
Lys
390
<210>128
<211>390
<212>PRT
<213>谷氨酸棒状杆菌
<400>128
Leu Ile Val Val Gly Ile Tyr Tyr Phe Asn Gly Glu Met His Phe Trp
1 5 10 15
Gln Gly Leu Leu Ile Ile Gly Phe Ser Phe Ile Pro Ala Trp Leu Ser
20 25 30
Tyr His Phe Ile Glu Asn Pro Met Arg Asn Trp Ser Ala Ile Lys Ala
35 40 45
Asp Ala Arg Lys Ala Val Ala Phe Gly Val Ile Leu Met Thr Val Ser
50 55 60
Ala Thr Ala Gly Ile Leu Leu Phe Leu Ser Pro Asn Arg Ser Gln Ala
65 70 75 80
Ala Pro Pro Gln Phe Ser Leu Thr Pro Tyr Asp Pro Thr Ala Val Asn
85 90 95
Lys Glu Ser Glu Lys Glu Ala Ala Lys Asn Leu Phe Gly Ala Glu Ala
100 105 110
Leu Thr Val Asp Pro Asp Ala Gly Glu Val Val Asp Arg Val Asp Asn
115 120 125
Phe Tyr Pro Thr Thr Ala Lys Ala Lys Arg Asp Tyr Pro Ser Asn Tyr
130 135 140
Ala Ala Gly Cys His Gln Glu Val Asn Glu Thr Ser Pro Glu Ser Cys
145 150 155 160
Val Tyr Gly Asp Lys Asn Ser Asp Phe Ser Val Ala Leu Val Gly Asp
165 170 175
Ser His Ala Gly His Trp Leu Pro Ala Leu Glu Pro Ile Ala Glu Ala
180 185 190
Gln Gly Trp Arg Leu Glu Val Tyr Thr Lys Ser Gln Cys Pro Leu Ile
195 200 205
Ser Thr Ala Ile Lys Leu Gly Glu Thr Phe Tyr Ala Glu Cys Tyr Glu
210 215 220
Trp Asn Glu Lys Leu Leu Ala Lys Leu Thr Gly Pro Ser Ala Pro Asn
225 230 235 240
His Val Ile Val Ser Ser Gln Arg Tyr Ala Ser Ala Asn Pro Leu Ile
245 250 255
Asp Ser Val Ala Thr Gly Thr Val Ser Glu Gly Tyr Glu Met Ala Trp
260 265 270
Asn Ser Leu Lys Asp Ala Gly Val Ser Ile Ser Val Leu Leu Asp Thr
275 280 285
Pro Arg Pro Gln Ile Asp Ile Pro Glu Cys Val Ala Ser Asn Arg Asp
290 295 300
Asn Leu Ser Glu Cys Ser Val His Arg Ser Val Ala Leu Gly Thr Glu
305 310 315 320
Ala His Pro Gln Gln Lys Thr Ala Ala Gln Asn Ile Asp Val Pro Val
325 330 335
Leu Asp Leu Ser Asn Trp Ile Cys Pro Glu Glu Tyr Cys Ser Ala Val
340 345 350
Ile Gly Asn Val Leu Val Tyr Arg Asp Ser His His Leu Thr Ala Thr
355 360 365
Tyr Ala Arg Ser Leu Ser Ser Ala Leu Trp Asn Glu Leu Val Ala Ser
370 375 380
Asn Gly Glu Pro Phe Lys
385 390
<210>129
<211>1246
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1216)
<223>RXA01268
<400>129
gtagtaaccg atacgattta ttgaagctat ttagtaattg aggacaggtg tgtcaatgat 60
actaagaaat aggtcacgtt gtagatcttc tagagagaat gtg att ctc ttt ggg 115
Val Ile Leu Phe Gly
1 5
gct acg aca gac atg tca aat att ttt tat gaa ggg atc ctg cag aat 163
Ala Thr Thr Asp Met Ser Asn Ile Phe Tyr Glu Gly Ile Leu Gln Asn
10 15 20
cta att caa gat ggg tgg gat gtg cac ctt gtt tca aat cca ggc cca 211
Leu Ile Gln Asp Gly Trp Asp Val His Leu Val Ser Asn Pro Gly Pro
25 30 35
gtt ggg aag aaa tta ctg aat ggt aaa gct cat act atc gag atg tct 259
Val Gly Lys Lys Leu Leu Asn Gly Lys Ala His Thr Ile Glu Met Ser
40 45 50
aga gag att tca ata ggt acc gat gtt aaa tca tta ttt aat tgg gtg 307
Arg Glu Ile Ser Ile Gly Thr Asp Val Lys Ser Leu Phe Asn Trp Val
55 60 65
cta ttg ctt agg aaa att cgt cca aga gtg ctg ata gtt gga act ccg 355
Leu Leu Leu Arg Lys Ile Arg Pro Arg Val Leu Ile Val Gly Thr Pro
70 75 80 85
aaa gct tca ttg ctg gga gtc gtg gca gca cga att gct cga gtg cca 403
Lys Ala Ser Leu Leu Gly Val Val Ala Ala Arg Ile Ala Arg Val Pro
90 95 100
cga att gtc tat gtg gca cat ggg ctg cgc tct gaa act gtt tta ggc 451
Arg Ile Val Tyr Val Ala His Gly Leu Arg Ser Glu Thr Val Leu Gly
105 110 115
tta aaa aag aag att cta gtg ttt ttg gaa tat ttg acc cag ttg ttt 499
Leu Lys Lys Lys Ile Leu Val Phe Leu Glu Tyr Leu Thr Gln Leu Phe
120 125 130
gca cat caa act ttg gct gta agt cat tct ctg aag aaa gca att gaa 547
Ala His Gln Thr Leu Ala Val Ser His Ser Leu Lys Lys Ala Ile Glu
135 140 145
gat gcg cac cct cgt ttt aaa gga aga gtg caa gtc tta ggt tat ggc 595
Asp Ala His Pro Arg Phe Lys Gly Arg Val Gln Val Leu Gly Tyr Gly
150 155 160 165
agt atg aat gga gtt gag ctt gat cgc ttt aga gtt cca tcc ctt gaa 643
Ser Met Asn Gly Val Glu Leu Asp Arg Phe Arg Val Pro Ser Leu Glu
170 175 180
gag aaa tta tct gct cgt aat gct tta aac ctg cct agt aaa tct gtc 691
Glu Lys Leu Ser Ala Arg Asn Ala Leu Asn Leu Pro Ser Lys Ser Val
185 190 195
att gtt ggt ttt gtc ggc aga ata aat aaa gat aag gga gga gat ctt 739
Ile Val Gly Phe Val Gly Arg Ile Asn Lys Asp Lys Gly Gly Asp Leu
200 205 210
ctc gct gct ctt aca aaa cat gag gct ttt acc cga ttg cga ctg cat 787
Leu Ala Ala Leu Thr Lys His Glu Ala Phe Thr Arg Leu Arg Leu His
215 220 225
ctc tta att att ggt gaa ttg gaa gac gat gac ttg cga gaa gca ttc 835
Leu Leu Ile Ile Gly Glu Leu Glu Asp Asp Asp Leu Arg Glu Ala Phe
230 235 240 245
att aaa tta gtt aat gaa ggg cag gtt acg att aca gga tgg att gat 883
Ile Lys Leu Val Asn Glu Gly Gln Val Thr Ile Thr Gly Trp Ile Asp
250 255 260
ttc cct gaa gaa cca tta gct gca gtt gat gtt ttg ctt cac cca act 931
Phe Pro Glu Glu Pro Leu Ala Ala Val Asp Val Leu Leu His Pro Thr
265 270 275
cag cga gaa ggt tta ggt atg tct ttg ctg gaa gct cag gct atg gga 979
Gln Arg Glu Gly Leu Gly Met Ser Leu Leu Glu Ala Gln Ala Met Gly
280 285 290
gtg cct gta ttg acg aat gct gtg act gga aca gtt gat gca gta aca 1027
Val Pro Val Leu Thr Asn Ala Val Thr Gly Thr Val Asp Ala Val Thr
295 300 305
agt gga gaa ggt ggc ttt ttt gcc gat gac gat tct gtt gag tcc tgg 1075
Ser Gly Glu Gly Gly Phe Phe Ala Asp Asp Asp Ser Val Glu Ser Trp
310 315 320 325
gtt tct aag att gat tta tta gtt tcc gat cct aag tta aga gac cgg 1123
Val Ser Lys Ile Asp Leu Leu Val Ser Asp Pro Lys Leu Arg Asp Arg
330 335 340
atg gga cgt gct ggt cgc cag ttt gtg tca gct cgt ttc aat cgt gat 1171
Met Gly Arg Ala Gly Arg Gln Phe Val Ser Ala Arg Phe Asn Arg Asp
345 350 355
gat gtc gca gct cgt ttc agt cat ttc gtg gaa caa ttc aaa aaa 1216
Asp Val Ala Ala Arg Phe Ser His Phe Val Glu Gln Phe Lys Lys
360 365 370
tagggctcat tcaatttcaa tattcagata 1246
<210>130
<211>372
<212>PRT
<213>谷氨酸棒状杆菌
<400>130
Val Ile Leu Phe Gly Ala Thr Thr Asp Met Ser Asn Ile Phe Tyr Glu
1 5 10 15
Gly Ile Leu Gln Asn Leu Ile Gln Asp Gly Trp Asp Val His Leu Val
20 25 30
Ser Asn Pro Gly Pro Val Gly Lys Lys Leu Leu Asn Gly Lys Ala His
35 40 45
Thr Ile Glu Met Ser Arg Glu Ile Ser Ile Gly Thr Asp Val Lys Ser
50 55 60
Leu Phe Asn Trp Val Leu Leu Leu Arg Lys Ile Arg Pro Arg Val Leu
65 70 75 80
Ile Val Gly Thr Pro Lys Ala Ser Leu Leu Gly Val Val Ala Ala Arg
85 90 95
Ile Ala Arg Val Pro Arg Ile Val Tyr Val Ala His Gly Leu Arg Ser
100 105 110
Glu Thr Val Leu Gly Leu Lys Lys Lys Ile Leu Val Phe Leu Glu Tyr
115 120 125
Leu Thr Gln Leu Phe Ala His Gln Thr Leu Ala Val Ser His Ser Leu
130 135 140
Lys Lys Ala Ile Glu Asp Ala His Pro Arg Phe Lys Gly Arg Val Gln
145 150 155 160
Val Leu Gly Tyr Gly Ser Met Asn Gly Val Glu Leu Asp Arg Phe Arg
165 170 175
Val Pro Ser Leu Glu Glu Lys Leu Ser Ala Arg Asn Ala Leu Asn Leu
180 185 190
Pro Ser Lys Ser Val Ile Val Gly Phe Val Gly Arg Ile Asn Lys Asp
195 200 205
Lys Gly Gly Asp Leu Leu Ala Ala Leu Thr Lys His Glu Ala Phe Thr
210 215 220
Arg Leu Arg Leu His Leu Leu Ile Ile Gly Glu Leu Glu Asp Asp Asp
225 230 235 240
Leu Arg Glu Ala Phe Ile Lys Leu Val Asn Glu Gly Gln Val Thr Ile
245 250 255
Thr Gly Trp Ile Asp Phe Pro Glu Glu Pro Leu Ala Ala Val Asp Val
260 265 270
Leu Leu His Pro Thr Gln Arg Glu Gly Leu Gly Met Ser Leu Leu Glu
275 280 285
Ala Gln Ala Met Gly Val Pro Val Leu Thr Asn Ala Val Thr Gly Thr
290 295 300
Val Asp Ala Val Thr Ser Gly Glu Gly Gly Phe Phe Ala Asp Asp Asp
305 310 315 320
Ser Val Glu Ser Trp Val Ser Lys Ile Asp Leu Leu Val Ser Asp Pro
325 330 335
Lys Leu Arg Asp Arg Met Gly Arg Ala Gly Arg Gln Phe Val Ser Ala
340 345 350
Arg Phe Asn Arg Asp Asp Val Ala Ala Arg Phe Ser His Phe Val Glu
355 360 365
Gln Phe Lys Lys
370
<210>131
<211>1942
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1912)
<223>RXA01271
<400>131
gtgaatccga tgccggacag atcgcagcaa ctcaatttgg agattcagtt ttagcctggg 60
ttggcgaaac ccaatttatt tcaaaagatg gtggacaaac atg gca ata agc att 115
Met Ala Ile Ser Ile
l 5
ggt aaa gca gga cag aac tta aaa ggg tca gtg cct atc gga aaa gtc 163
Gly Lys Ala Gly Gln Asn Leu Lys Gly Ser Val Pro Ile Gly Lys Val
10 15 20
ctt ttt ctc att gat gcc ctt gcc tgg att tct gca cta ttt atc ggt 211
Leu Phe Leu Ile Asp Ala Leu Ala Trp Ile Ser Ala Leu Phe Ile Gly
25 30 35
gtg gtt ttg cgg tat gaa ttc aat ctg agt tct atc aac tgg agt gcg 259
Val Val Leu Arg Tyr Glu Phe Asn Leu Ser Ser Ile Asn Trp Ser Ala
40 45 50
ttt gca tgg ttc ggg ctt gct gca gtt att ttg cag ttt gtt ctc gga 307
Phe Ala Trp Phe Gly Leu Ala Ala Val Ile Leu Gln Phe Val Leu Gly
55 60 65
ctt tct ctc cat ctt tac cgc aag gga tta cgt cac ctt ttc ggt agc 355
Leu Ser Leu His Leu Tyr Arg Lys Gly Leu Arg His Leu Phe Gly Ser
70 75 80 85
ttc gaa gat aca cta aac gtt tct atc tcg gtc att gtt gtc ggt gtt 403
Phe Glu Asp Thr Leu Asn Val Ser Ile Ser Val Ile Val Val Gly Val
90 95 100
gtc ctt tgg atc gcc tca cta ttt gtt ggt cag cgt tgg gaa atc tca 451
Val Leu Trp Ile Ala Ser Leu Phe Val Gly Gln Arg Trp Glu Ile Ser
105 110 115
cgc ggt gtc atg ctg cta gtt atc ccg ctt gct ctc gta ttc gta ttg 499
Arg Gly Val Met Leu Leu Val Ile Pro Leu Ala Leu Val Phe Val Leu
120 125 130
gca gtg cgt tat ctc gcg cgt atg cga gtt gag cgt ctt cgt cgt ccg 547
Ala Val Arg Tyr Leu Ala Arg Met Arg Val Glu Arg Leu Arg Arg Pro
135 140 145
gct gcg gat tcc aca cca gca ttg att ctt ggt ggt gga tac atc ggt 595
Ala Ala Asp Ser Thr Pro Ala Leu Ile Leu Gly Gly Gly Tyr Ile Gly
150 155 160 165
acc aac ctg atc cag tgg atg atg tcc gat cct aag tcg cct ttc cgc 643
Thr Asn Leu Ile Gln Trp Met Met Ser Asp Pro Lys Ser Pro Phe Arg
170 175 180
cca gtc ggc gtt att gat gat aac cct gaa tta gca tgg caa cgc gta 691
Pro Val Gly Val Ile Asp Asp Asn Pro Glu Leu Ala Trp Gln Arg Val
185 190 195
cgt ggt gtg ccg gtt ctt ggc aag ttt gat gat atc gcc caa gtt gca 739
Arg Gly Val Pro Val Leu Gly Lys Phe Asp Asp Ile Ala Gln Val Ala
200 205 210
tca gac act ggc gca gaa ctt ctt atc gtt gct att ggt gat gcc gac 787
Ser Asp Thr Gly Ala Glu Leu Leu Ile Val Ala Ile Gly Asp Ala Asp
215 220 225
tct gca ctt tta agg cgt gtc caa gat acc gct aat aaa aat ggt ctt 835
Ser Ala Leu Leu Arg Arg Val Gln Asp Thr Ala Asn Lys Asn Gly Leu
230 235 240 245
tca gta aag gta atg ccg gct att gac cgc gtc gtt tct aag ggc gtt 883
Ser Val Lys Val Met Pro Ala Ile Asp Arg Val Val Ser Lys Gly Val
250 255 260
cgt gga aac gat ttg cgt gat ctc tct att gaa gat ttg ctt gga cgt 931
Arg Gly Asn Asp Leu Arg Asp Leu Ser Ile Glu Asp Leu Leu Gly Arg
265 270 275
caa cct gtt gaa acc aat gtt tca gaa att act ggc tat cta aca ggt 979
Gln Pro Val Glu Thr Asn Val Ser Glu Ile Thr Gly Tyr Leu Thr Gly
280 285 290
aag cgt gtt ctt gtt acc ggt gca ggt ggg tca att ggt tcc cag cta 1027
Lys Arg Val Leu Val Thr Gly Ala Gly Gly Ser Ile Gly Ser Gln Leu
295 300 305
tgt acg gaa att gcc aaa tac gga cct gct gag ctt atg atg ctt gat 1075
Cys Thr Glu Ile Ala Lys Tyr Gly Pro Ala Glu Leu Met Met Leu Asp
310 315 320 325
cgc gat gag act ggt ttg cag cag gtt ctg att aac gtt gct ggt aac 1123
Arg Asp Glu Thr Gly Leu Gln Gln Val Leu Ile Asn Val Ala Gly Asn
330 335 340
ggt ttg ttg gat acg gat gct gtg gtt ctt gcg gat atc cgc gaa gca 1171
Gly Leu Leu Asp Thr Asp Ala Val Val Leu Ala Asp Ile Arg Glu Ala
345 350 355
gac gcg atg aaa gag att ttt ctc aag cgt aaa cca gaa gtt gtc ttc 1219
Asp Ala Met Lys Glu Ile Phe Leu Lys Arg Lys Pro Glu Val Val Phe
360 365 370
cat gca gca gca tta aag cac ttg cca atg ctg gag cag tat cca gat 1267
His Ala Ala Ala Leu Lys His Leu Pro Met Leu Glu Gln Tyr Pro Asp
375 380 385
gag ggc tgg aaa aca aac gtt cta gga act ctt aac gtt ctt gct gcc 1315
Glu Gly Trp Lys Thr Asn Val Leu Gly Thr Leu Asn Val Leu Ala Ala
390 395 400 405
gca gaa gct gtt ggt gtt gag act ttc gtc aat att tcc acc gat aag 1363
Ala Glu Ala Val Gly Val Glu Thr Phe Val Asn Ile Ser Thr Asp Lys
410 415 420
gca gct aat cca acc agc gtc tta ggg cac tca aag cga gtc gct gaa 141l
Ala Ala Asn Pro Thr Ser Val Leu Gly His Ser Lys Arg Val Ala Glu
425 430 435
aag ctg act gcc tgg tat gga cag aat tcc acc agc aag tac cta tcg 1459
Lys Leu Thr Ala Trp Tyr Gly Gln Asn Ser Thr Ser Lys Tyr Leu Ser
440 445 450
gtt cga ttt gga aac gtc att ggt agc cgt gga tcg atg ctc ccg act 1507
Val Arg Phe Gly Asn Val Ile Gly Ser Arg Gly Ser Met Leu Pro Thr
455 460 465
ttc acc agg cta atc atg gaa gat aaa ccg cta aca gtg acg cac ccg 1555
Phe Thr Arg Leu Ile Met Glu Asp Lys Pro Leu Thr Val Thr His Pro
470 475 480 485
gat gtc act agg ttc ttc atg aca att cct gaa gct tgc caa ttg gtc 1603
Asp Val Thr Arg Phe Phe Met Thr Ile Pro Glu Ala Cys Gln Leu Val
490 495 500
ctg caa gcc ggt ggt att gga cgt tcc ggc gag gtt ctc atc ctt gac 165l
Leu Gln Ala Gly Gly Ile Gly Arg Ser Gly Glu Val Leu Ile Leu Asp
505 510 515
atg ggt gag cct gta agc atc ctt gaa att gca cag cgc atg atc gca 1699
Met Gly Glu Pro Val Ser Ile Leu Glu Ile Ala Gln Arg Met Ile Ala
520 525 530
atg tcc ggt aaa gat att gac atc gtg ttc acc ggc ctt cgc gag ggc 1747
Met Ser Gly Lys Asp Ile Asp Ile Val Phe Thr Gly Leu Arg Glu Gly
535 540 545
gaa aag atg cac gaa gag ctg gtt ggt gat ggt gaa acc gaa gat cgt 1795
Glu Lys Met His Glu Glu Leu Val Gly Asp Gly Glu Thr Glu Asp Arg
550 555 560 565
cca ttc cac tca aag att tcg cat gca cat gca gaa agc ctc gct cct 1843
Pro Phe His Ser Lys Ile Ser His Ala His Ala Glu Ser Leu Ala Pro
570 575 580
aat aat ctc gat aga gat cga ttt atg caa cgt gct gga aaa cta gct 1891
Asn Asn Leu Asp Arg Asp Arg Phe Met Gln Arg Ala Gly Lys Leu Ala
585 590 595
tca aca gat tcg gag atc atc taaatgacta atgaacgaat ttttctatca 1942
Ser Thr Asp Ser Glu Ile Ile
600
<210>132
<211>604
<212>PRT
<213>谷氨酸棒状杆菌
<400>132
Met Ala Ile Ser Ile Gly Lys Ala Gly Gln Asn Leu Lys Gly Ser Val
1 5 10 15
Pro Ile Gly Lys Val Leu Phe Leu Ile Asp Ala Leu Ala Trp Ile Ser
20 25 30
Ala Leu Phe Ile Gly Val Val Leu Arg Tyr Glu Phe Asn Leu Ser Ser
35 40 45
Ile Asn Trp Ser Ala Phe Ala Trp Phe Gly Leu Ala Ala Val Ile Leu
50 55 60
Gln Phe Val Leu Gly Leu Ser Leu His Leu Tyr Arg Lys Gly Leu Arg
65 70 75 80
His Leu Phe Gly Ser Phe Glu Asp Thr Leu Asn Val Ser Ile Ser Val
85 90 95
Ile Val Val Gly Val Val Leu Trp Ile Ala Ser Leu Phe Val Gly Gln
100 105 110
Arg Trp Glu Ile Ser Arg Gly Val Met Leu Leu Val Ile Pro Leu Ala
115 120 125
Leu Val Phe Val Leu Ala Val Arg Tyr Leu Ala Arg Met Arg Val Glu
130 135 140
Arg Leu Arg Arg Pro Ala Ala Asp Ser Thr Pro Ala Leu Ile Leu Gly
145 150 155 160
Gly Gly Tyr Ile Gly Thr Asn Leu Ile Gln Trp Met Met Ser Asp Pro
165 170 175
Lys Ser Pro Phe Arg Pro Val Gly Val Ile Asp Asp Asn Pro Glu Leu
180 185 190
Ala Trp Gln Arg Val Arg Gly Val Pro Val Leu Gly Lys Phe Asp Asp
195 200 205
Ile Ala Gln Val Ala Ser Asp Thr Gly Ala Glu Leu Leu Ile Val Ala
210 215 220
Ile Gly Asp Ala Asp Ser Ala Leu Leu Arg Arg Val Gln Asp Thr Ala
225 230 235 240
Asn Lys Asn Gly Leu Ser Val Lys Val Met Pro Ala Ile Asp Arg Val
245 250 255
Val Ser Lys Gly Val Arg Gly Asn Asp Leu Arg Asp Leu Ser Ile Glu
260 265 270
Asp Leu Leu Gly Arg Gln Pro Val Glu Thr Asn Val Ser Glu Ile Thr
275 280 285
Gly Tyr Leu Thr Gly Lys Arg Val Leu Val Thr Gly Ala Gly Gly Ser
290 295 300
Ile Gly Ser Gln Leu Cys Thr Glu Ile Ala Lys Tyr Gly Pro Ala Glu
305 310 315 320
Leu Met Met Leu Asp Arg Asp Glu Thr Gly Leu Gln Gln Val Leu Ile
325 330 335
Asn Val Ala Gly Asn Gly Leu Leu Asp Thr Asp Ala Val Val Leu Ala
340 345 350
Asp Ile Arg Glu Ala Asp Ala Met Lys Glu Ile Phe Leu Lys Arg Lys
355 360 365
Pro Glu Val Val Phe His Ala Ala Ala Leu Lys His Leu Pro Met Leu
370 375 380
Glu Gln Tyr Pro Asp Glu Gly Trp Lys Thr Asn Val Leu Gly Thr Leu
385 390 395 400
Asn Val Leu Ala Ala Ala Glu Ala Val Gly Val Glu Thr Phe Val Asn
405 410 415
Ile Ser Thr Asp Lys Ala Ala Asn Pro Thr Ser Val Leu Gly His Ser
420 425 430
Lys Arg Val Ala Glu Lys Leu Thr Ala Trp Tyr Gly Gln Asn Ser Thr
435 440 445
Ser Lys Tyr Leu Ser Val Arg Phe Gly Asn Val Ile Gly Ser Arg Gly
450 455 460
Ser Met Leu Pro Thr Phe Thr Arg Leu Ile Met Glu Asp Lys Pro Leu
465 470 475 480
Thr Val Thr His Pro Asp Val Thr Arg Phe Phe Met Thr Ile Pro Glu
485 490 495
Ala Cys Gln Leu Val Leu Gln Ala Gly Gly Ile Gly Arg Ser Gly Glu
500 505 510
Val Leu Ile Leu Asp Met Gly Glu Pro Val Ser Ile Leu Glu Ile Ala
515 520 525
Gln Arg Met Ile Ala Met Ser Gly Lys Asp Ile Asp Ile Val Phe Thr
530 535 540
Gly Leu Arg Glu Gly Glu Lys Met His Glu Glu Leu Val Gly Asp Gly
545 550 555 560
Glu Thr Glu Asp Arg Pro Phe His Ser Lys Ile Ser His Ala His Ala
565 570 575
Glu Ser Leu Ala Pro Asn Asn Leu Asp Arg Asp Arg Phe Met Gln Arg
580 585 590
Ala Gly Lys Leu Ala Ser Thr Asp Ser Glu Ile Ile
595 600
<210>133
<211>1564
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1534)
<223>RXA01273
<00>133
gacctattca gcgctggatt ttttctggca ttttcttggc agtcagtgag ttgctaacgt 60
tctattggtt aaaaattgat ctgcaagagg actgcaaaat atg gaa tta agg gaa 115
Met Glu Leu Arg Glu
1 5
tac gcg aca atc ctg atg aag aat tgg gtg ttg atc gtt atc gca tcc 163
Tyr Ala Thr Ile Leu Met Lys Asn Trp Val Leu Ile Val Ile Ala Ser
10 15 20
att ctt gga att gca gcg ggc gct ggt ttt tcc ctt cta gct aca ccg 211
Ile Leu Gly Ile Ala Ala Gly Ala Gly Phe Ser Leu Leu Ala Thr Pro
25 30 35
gag tat cag tca cgt act cag ttg tat gta tcg gtg cgg tcg ggg gct 259
Glu Tyr Gln Ser Arg Thr Gln Leu Tyr Val Ser Val Arg Ser Gly Ala
40 45 50
ggg aca acc tct gac atg gtt cag ggt gct aac ttt tcg cgt cag att 307
Gly Thr Thr Ser Asp Met Val Gln Gly Ala Asn Phe Ser Arg Gln Ile
55 60 65
gtg aat agt tat gtt gat gtc att aag acg ggt gtt gtt cta gag ccg 355
Val Asn Ser Tyr Val Asp Val Ile Lys Thr Gly Val Val Leu Glu Pro
70 75 80 85
gtt gtt gat gag ctt ggt ctg gag ttg acg gct aac cag ttg agt tct 403
Val Val Asp Glu Leu Gly Leu Glu Leu Thr Ala Asn Gln Leu Ser Ser
90 95 100
cat atc agt gcg gct tct cct gct gat act gcg ttg atc aac atc act 451
His Ile Ser Ala Ala Ser Pro Ala Asp Thr Ala Leu Ile Asn Ile Thr
105 110 115
gct tct agt cct tct cct cag cag gcg gct gag atc gcc aat gca gtg 499
Ala Ser Ser Pro Ser Pro Gln Gln Ala Ala Glu Ile Ala Asn Ala Val
120 125 130
ggg gag agt ttc aag aat gtg att cag act gaa ttg gaa cca gac tcc 547
Gly Glu Ser Phe Lys Asn Val Ile Gln Thr Glu Leu Glu Pro Asp Ser
135 140 145
ggc gat ggc atg agc ccg att aac ctg act act act cag gtg gct ctg 595
Gly Asp Gly Met Ser Pro Ile Asn Leu Thr Thr Thr Gln Val Ala Leu
150 155 160 165
gaa cca tct tct ccg gtc agt cct aat gtg atg atg aat atc ctc ctc 643
Glu Pro Ser Ser Pro Val Ser Pro Asn Val Met Met Asn Ile Leu Leu
170 175 180
ggc cta ctt gta ggt ctt gca ata ggt gtt ggt att gca gtg ctt cgc 691
Gly Leu Leu Val Gly Leu Ala Ile Gly Val Gly Ile Ala Val Leu Arg
185 190 195
gcg gct ttg gat act cgt att cat tcc ttg cgc gat att gaa gag gtt 739
Ala Ala Leu Asp Thr Arg Ile His Ser Leu Arg Asp Ile Glu Glu Val
200 205 210
act gat aag cca ctt ttg ggc gga atc att gcg gat tct gaa gtt gag 787
Thr Asp Lys Pro Leu Leu Gly Gly Ile Ile Ala Asp Ser Glu Val Glu
215 220 225
aag cac ccg ttg atc att aag cac aag ccg cat agt cct att gcg gag 835
Lys His Pro Leu Ile Ile Lys His Lys Pro His Ser Pro Ile Ala Glu
230 235 240 245
tcc ttt cgt gcg tta cgt act aac ctg cag ttc ctt aac gtc ggc ggc 883
Ser Phe Arg Ala Leu Arg Thr Asn Leu Gln Phe Leu Asn Val Gly Gly
250 255 260
tca tct tca gta ttt gtt atc tcc tct gct aat cct ggt gag ggt aag 931
Ser Ser Ser Val Phe Val Ile Ser Ser Ala Asn Pro Gly Glu Gly Lys
265 270 275
tca acc act tct gta aac cta gct ttg gcg ctt gca gag gcc ggc tcc 979
Ser Thr Thr Ser Val Asn Leu Ala Leu Ala Leu Ala Glu Ala Gly Ser
280 285 290
cgt gta gcg ctg atc gaa gct gat ctt cgc ttg cca cga gtg agc aag 1027
Arg Val Ala Leu Ile Glu Ala Asp Leu Arg Leu Pro Arg Val Ser Lys
295 300 305
tac ctc gga gtt gag ggc aac gca ggt ctg act gac att ctc att ggc 1075
Tyr Leu Gly Val Glu Gly Asn Ala Gly Leu Thr Asp Ile Leu Ile Gly
310 315 320 325
aag gcc gag gtt aat gat gtg ttg cag cgt tgg ggt agg act cag ttg 1123
Lys Ala Glu Val Asn Asp Val Leu Gln Arg Trp Gly Arg Thr Gln Leu
330 335 340
tac tat ctc ccg gca ggg cgc att ccg ccg aac ccg agt gag ttg ctt 1171
Tyr Tyr Leu Pro Ala Gly Arg Ile Pro Pro Asn Pro Ser Glu Leu Leu
345 350 355
ggt tca gct gag atg gac aag gtc att gcg gag ctt gag gaa agc ttt 1219
Gly Ser Ala Glu Met Asp Lys Val Ile Ala Glu Leu Glu Glu Ser Phe
360 365 370
gat tat gtg atc atc gat gcc cct ccg gcg ttg gcg gtt acc gat gct 1267
Asp Tyr Val Ile Ile Asp Ala Pro Pro Ala Leu Ala Val Thr Asp Ala
375 380 385
gca gtt att ggt cat ggc aag gct ggc atc ctg att gcg gtc tcc gca 1315
Ala Val Ile Gly His Gly Lys Ala Gly Ile Leu Ile Ala Val Ser Ala
390 395 400 405
ggt tct aca aag aag cct gag ttg gaa gct acg ctg tcc acg ctt gag 1363
Gly Ser Thr Lys Lys Pro Glu Leu Glu Ala Thr Leu Ser Thr Leu Glu
410 415 420
aat gcg gat gcc aat gtt gtt ggc gtt gtc gct acg atg ctc ccg cct 1411
Asn Ala Asp Ala Asn Val Val Gly Val Val Ala Thr Met Leu Pro Pro
425 430 435
aag tct gtg gct ggt tat ggc tac gga aat tac ggc tac ggc gac acc 1459
Lys Ser Val Ala Gly Tyr Gly Tyr Gly Asn Tyr Gly Tyr Gly Asp Thr
440 445 450
tcc aaa atc aat gcc cct aag ccc gac aac acc gaa cta acc acc acc 1507
Ser Lys Ile Asn Ala Pro Lys Pro Asp Asn Thr Glu Leu Thr Thr Thr
455 460 465
gat gct tcc aag gcc aac aat gag caa tagcttcact attctcactg 1554
Asp Ala Ser Lys Ala Asn Asn Glu Gln
470 475
tctgtactgg 1564
<210>134
<211>478
<212>PRT
<213>谷氨酸棒状杆菌
<400>134
Met Glu Leu Arg Glu Tyr Ala Thr Ile Leu Met Lys Asn Trp Val Leu
1 5 10 15
Ile Val Ile Ala Ser Ile Leu Gly Ile Ala Ala Gly Ala Gly Phe Ser
20 25 30
Leu Leu Ala Thr Pro Glu Tyr Gln Ser Arg Thr Gln Leu Tyr Val Ser
35 40 45
Val Arg Ser Gly Ala Gly Thr Thr Ser Asp Met Val Gln Gly Ala Asn
50 55 60
Phe Ser Arg Gln Ile Val Asn Ser Tyr Val Asp Val Ile Lys Thr Gly
65 70 75 80
Val Val Leu Glu Pro Val Val Asp Glu Leu Gly Leu Glu Leu Thr Ala
85 90 95
Asn Gln Leu Ser Ser His Ile Ser Ala Ala Ser Pro Ala Asp Thr Ala
100 105 110
Leu Ile Asn Ile Thr Ala Ser Ser Pro Ser Pro Gln Gln Ala Ala Glu
115 120 125
Ile Ala Asn Ala Val Gly Glu Ser Phe Lys Asn Val Ile Gln Thr Glu
130 135 140
Leu Glu Pro Asp Ser Gly Asp Gly Met Ser Pro Ile Asn Leu Thr Thr
145 150 155 160
Thr Gln Val Ala Leu Glu Pro Ser Ser Pro Val Ser Pro Asn Val Met
165 170 175
Met Asn Ile Leu Leu Gly Leu Leu Val Gly Leu Ala Ile Gly Val Gly
180 185 190
Ile Ala Val Leu Arg Ala Ala Leu Asp Thr Arg Ile His Ser Leu Arg
195 200 205
Asp Ile Glu Glu Val Thr Asp Lys Pro Leu Leu Gly Gly Ile Ile Ala
210 215 220
Asp Ser Glu Val Glu Lys His Pro Leu Ile Ile Lys His Lys Pro His
225 230 235 240
Ser Pro Ile Ala Glu Ser Phe Arg Ala Leu Arg Thr Asn Leu Gln Phe
245 250 255
Leu Asn Val Gly Gly Ser Ser Ser Val Phe Val Ile Ser Ser Ala Asn
260 265 270
Pro Gly Glu Gly Lys Ser Thr Thr Ser Val Asn Leu Ala Leu Ala Leu
275 280 285
Ala Glu Ala Gly Ser Arg Val Ala Leu Ile Glu Ala Asp Leu Arg Leu
290 295 300
Pro Arg Val Ser Lys Tyr Leu Gly Val Glu Gly Asn Ala Gly Leu Thr
305 310 315 320
Asp Ile Leu Ile Gly Lys Ala Glu Val Asn Asp Val Leu Gln Arg Trp
325 330 335
Gly Arg Thr Gln Leu Tyr Tyr Leu Pro Ala Gly Arg Ile Pro Pro Asn
340 345 350
Pro Ser Glu Leu Leu Gly Ser Ala Glu Met Asp Lys Val Ile Ala Glu
355 360 365
Leu Glu Glu Ser Phe Asp Tyr Val Ile Ile Asp Ala Pro Pro Ala Leu
370 375 380
Ala Val Thr Asp Ala Ala Val Ile Gly His Gly Lys Ala Gly Ile Leu
385 390 395 400
Ile Ala Val Ser Ala Gly Ser Thr Lys Lys Pro Glu Leu Glu Ala Thr
405 410 415
Leu Ser Thr Leu Glu Asn Ala Asp Ala Asn Val Val Gly Val Val Ala
420 425 430
Thr Met Leu Pro Pro Lys Ser Val Ala Gly Tyr Gly Tyr Gly Asn Tyr
435 440 445
Gly Tyr Gly Asp Thr Ser Lys Ile Asn Ala Pro Lys Pro Asp Asn Thr
450 455 460
Glu Leu Thr Thr Thr Asp Ala Ser Lys Ala Asn Asn Glu Gln
465 470 475
<210>135
<211>781
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(751)
<223>RXA01315
<400>135
catgtgcggt tgtgtcctaa gtcattgatg tcacctaacg cccctaagtt cactcggtgc 60
aatattgcac tgagtgcaag tttacactag gtttacttca gtg gat att gaa gag 115
Val Asp Ile Glu Glu
1 5
cag ccc tcg tta aga gaa atc aag cgc caa atg acc ctg gaa gcg ata 163
Gln Pro Ser Leu Arg Glu Ile Lys Arg Gln Met Thr Leu Glu Ala Ile
10 15 20
gaa gat aac gca acc agg ctc att ctg gag cgt ggc ttc gac aat gtc 211
Glu Asp Asn Ala Thr Arg Leu Ile Leu Glu Arg Gly Phe Asp Asn Val
25 30 35
aca atc gaa gac atc tgc gca gag gca ggg ata tcc aag cgc aca ttc 259
Thr Ile Glu Asp Ile Cys Ala Glu Ala Gly Ile Ser Lys Arg Thr Phe
40 45 50
ttt aac tac gtg gag tcc aaa gag tct gtg gcc atc ggg cac aca gcc 307
Phe Asn Tyr Val Glu Ser Lys Glu Ser Val Ala Ile Gly His Thr Ala
55 60 65
aag ctc cca acg gat gaa gaa cgt gaa gca ttc ctg gct acg cgt cat 355
Lys Leu Pro Thr Asp Glu Glu Arg Glu Ala Phe Leu Ala Thr Arg His
70 75 80 85
gaa aat att atc gat act gta ttt gac ctg gta atc aac ctc ttt ggc 403
Glu Asn Ile Ile Asp Thr Val Phe Asp Leu Val Ile Asn Leu Phe Gly
90 95 100
aac cac gac aac tcc aag tct gga gtt gca ggc gac att atg cgt cga 451
Asn His Asp Asn Ser Lys Ser Gly Val Ala Gly Asp Ile Met Arg Arg
105 110 115
cgc aaa gag atc cgg gtg aag cat cca gaa ctg gca gtg caa cat ttc 499
Arg Lys Glu Ile Arg Val Lys His Pro Glu Leu Ala Val Gln His Phe
120 125 130
gcc agg ttc cac caa gca cgc gaa ggg cta gaa cac cta att gtt gag 547
Ala Arg Phe His Gln Ala Arg Glu Gly Leu Glu His Leu Ile Val Glu
135 140 145
tac ttc gaa aaa tgg cca ggc tcc caa cat cta gat gag cct gca gat 595
Tyr Phe Glu Lys Trp Pro Gly Ser Gln His Leu Asp Glu Pro Ala Asp
150 155 160 165
cga gaa gca atc gcc ata gtt ggc ctg ctg atc tcg gtc atg ctt caa 643
Arg Glu Ala Ile Ala Ile Val Gly Leu Leu Ile Ser Val Met Leu Gln
170 175 180
ggt tct cgt gaa tgg cac gac atg cca caa ggc acg caa gct gat ttc 691
Gly Ser Arg Glu Trp His Asp Met Pro Gln Gly Thr Gln Ala Asp Phe
185 190 195
caa gcc tgc tgt cgc aaa gca att aaa aat act ttt ctt ctt aga ggt 739
Gln Ala Cys Cys Arg Lys Ala Ile Lys Asn Thr Phe Leu Leu Arg Gly
200 205 210
gga ttt tca gaa tgacatcaca ggtcaagccg gacgacgaac 781
Gly Phe Ser Glu
215
<210>136
<211>217
<212>PRT
<213>谷氨酸棒状杆菌
<400>136
Val Asp Ile Glu Glu Gln Pro Ser Leu Arg Glu Ile Lys Arg Gln Met
1 5 10 15
Thr Leu Glu Ala Ile Glu Asp Asn Ala Thr Arg Leu Ile Leu Glu Arg
20 25 30
Gly Phe Asp Asn Val Thr Ile Glu Asp Ile Cys Ala Glu Ala Gly Ile
35 40 45
Ser Lys Arg Thr Phe Phe Asn Tyr Val Glu Ser Lys Glu Ser Val Ala
50 55 60
Ile Gly His Thr Ala Lys Leu Pro Thr Asp Glu Glu Arg Glu Ala Phe
65 70 75 80
Leu Ala Thr Arg His Glu Asn Ile Ile Asp Thr Val Phe Asp Leu Val
85 90 95
Ile Asn Leu Phe Gly Asn His Asp Asn Ser Lys Ser Gly Val Ala Gly
100 105 110
Asp Ile Met Arg Arg Arg Lys Glu Ile Arg Val Lys His Pro Glu Leu
115 120 125
Ala Val Gln His Phe Ala Arg Phe His Gln Ala Arg Glu Gly Leu Glu
130 135 140
His Leu Ile Val Glu Tyr Phe Glu Lys Trp Pro Gly Ser Gln His Leu
145 150 155 160
Asp Glu Pro Ala Asp Arg Glu Ala Ile Ala Ile Val Gly Leu Leu Ile
165 170 175
Ser Val Met Leu Gln Gly Ser Arg Glu Trp His Asp Met Pro Gln Gly
180 185 190
Thr Gln Ala Asp Phe Gln Ala Cys Cys Arg Lys Ala Ile Lys Asn Thr
195 200 205
Phe Leu Leu Arg Gly Gly Phe Ser Glu
210 215
<210>137
<211>1432
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1402)
<223>RXA01318
<400>137
cggacgcgtt ccagagaaag tgcaggcata acccctaaaa taccctgatc ttcccccgtg 60
tcctgccccc gtgtccaccc ctgcgtacat aataggacgc atg gga aaa cat gag 115
Met Gly Lys His Glu
1 5
gtt gct cag cag acg gtt ccg ggt cct tcg ccg gaa atg gaa gcg cag 163
Val Ala Gln Gln Thr Val Pro Gly Pro Ser Pro Glu Met Glu Ala Gln
10 15 20
cgg cgt aaa gag ttg cgc aag cac aag gcc att gcc sct ggc ctg ttg 211
Arg Arg Lys Glu Leu Arg Lys His Lys Ala Ile Ala Thr Gly Leu Leu
25 30 35
att ttt gct gcc gct gta tat ttt ctt tgc cgt ttc gtg gag acc cgt 259
Ile Phe Ala Ala Ala Val Tyr Phe Leu Cys Arg Phe Val Glu Thr Arg
40 45 50
ccg ggt gaa act gca gcg tgg gta ggt ttt gtg cgc gct gcg gca gag 307
Pro Gly Glu Thr Ala Ala Trp Val Gly Phe Val Arg Ala Ala Ala Glu
55 60 65
gcc gga atg att ggc ggg ttg gcc gac tgg ttc gcg gtc acc gcg ctg 355
Ala Gly Met Ile Gly Gly Leu Ala Asp Trp Phe Ala Val Thr Ala Leu
70 75 80 85
ttc cgt cat cca ttg tgg ctg cct att ccg cac act gcg att atc ccg 403
Phe Arg His Pro Leu Trp Leu Pro Ile Pro His Thr Ala Ile Ile Pro
90 95 100
cgc aag aaa gac cag tta ggt gag gcc tta agc ggg ttt gtg ggg gat 451
Arg Lys Lys Asp Gln Leu Gly Glu Ala Leu Ser Gly Phe Val Gly Asp
105 110 115
aac ttc cta aat gcc cag ctc att acg gaa aaa gtc tct cag gcg cgg 499
Asn Phe Leu Asn Ala Gln Leu Ile Thr Glu Lys Val Ser Gln Ala Arg
120 125 130
atc cca gag cgc gcc ggg gag tgg ctc gcc cag ccg gaa aac ggg gag 547
Ile Pro Glu Arg Ala Gly Glu Trp Leu Ala Gln Pro Glu Asn Gly Glu
135 140 145
aaa gtt tcg cgc gaa gtc ggc aaa ttg acc gct aat att gtg cgc gca 595
Lys Val Ser Arg Glu Val Gly Lys Leu Thr Ala Asn Ile Val Arg Ala
150 155 160 165
atc gat ccg tca gat gct gaa gcg gtg att aaa tct gcg gtg atc gac 643
Ile Asp Pro Ser Asp Ala Glu Ala Val Ile Lys Ser Ala Val Ile Asp
170 175 180
aag ctt gcg gaa ccc acc tgg ggc cca cca gct ggg cgg ttg ctg gaa 691
Lys Leu Ala Glu Pro Thr Trp Gly Pro Pro Ala Gly Arg Leu Leu Glu
185 190 195
caa ctc ctc gcc gaa ggc aaa gcc gaa cca gtt gtc cag gaa ctc gcg 739
Gln Leu Leu Ala Glu Gly Lys Ala Glu Pro Val Val Gln Glu Leu Ala
200 205 210
cag tgg ctg cac aaa aag gcg ttg ggc tcc gag ccg ctg att gat cgc 787
Gln Trp Leu His Lys Lys Ala Leu Gly Ser Glu Pro Leu Ile Asp Arg
215 220 225
ctg ctc aac gag cgc cgc ccg att tgg gcg ccg aaa ttc act gcg cag 835
Leu Leu Asn Glu Arg Arg Pro Ile Trp Ala Pro Lys Phe Thr Ala Gln
230 235 240 245
ctg gtc agc ggc aaa gtc tat gac gag gtc ata aaa ttc act gaa gcc 883
Leu Val Ser Gly Lys Val Tyr Asp Glu Val Ile Lys Phe Thr Glu Ala
250 255 260
gtc gct gcc gat cct aac cac gag gcc cgc aaa tcg ctg cgc cga ttc 931
Val Ala Ala Asp Pro Asn His Glu Ala Arg Lys Ser Leu Arg Arg Phe
265 270 275
ctt aat aaa ttg gcg caa gac ctg cag cat gac cca ggc atg att att 979
Leu Asn Lys Leu Ala Gln Asp Leu Gln His Asp Pro Gly Met Ile Ile
280 285 290
aaa gtt gaa gaa atc aaa cgc gac atc atg ggc tcc ggc gcc atc gcg 1027
Lys Val Glu Glu Ile Lys Arg Asp Ile Met Gly Ser Gly Ala Ile Ala
295 300 305
caa gcc gcg cca acc atc tgg gcg tca gcc tcc gag tcg ctc att gaa 1075
Gln Ala Ala Pro Thr Ile Trp Ala Ser Ala Ser Glu Ser Leu Ile Glu
310 315 320 325
tcc gca gaa gat gag tca tca att ctg cgt cgc aaa att gcc gaa gca 1123
Ser Ala Glu Asp Glu Ser Ser Ile Leu Arg Arg Lys Ile Ala Glu Ala
330 335 340
gct acc agc tgg ggt caa aga ttg ctt gtc gac gac tcc ctc cgg cat 1171
Ala Thr Ser Trp Gly Gln Arg Leu Leu Val Asp Asp Ser Leu Arg His
345 350 355
tca ctc gac acc cgg att acc ggc gcc gct gct ttc ctc gcc gac aat 1219
Ser Leu Asp Thr Arg Ile Thr Gly Ala Ala Ala Phe Leu Ala Asp Asn
360 365 370
tac gcc ccc gaa gtc acc ggc att atc tcc gaa acc att gaa cga tgg 1267
Tyr Ala Pro Glu Val Thr Gly Ile Ile Ser Glu Thr Ile Glu Arg Trp
375 380 385
gac gct gaa gaa gct tca gag aaa atc gaa ctc atg gtg ggc aaa gac 1315
Asp Ala Glu Glu Ala Ser Glu Lys Ile Glu Leu Met Val Gly Lys Asp
390 395 400 405
ctc caa tac atc cgc ctt aat ggc aca att gta ggt gca tta gca gga 1363
Leu Gln Tyr Ile Arg Leu Asn Gly Thr Ile Val Gly Ala Leu Ala Gly
410 415 420
ctg gcc att tac gct att tcc cat atc ctc ttc gga gct taactaggag 1412
Leu Ala Ile Tyr Ala Ile Ser His Ile Leu Phe Gly Ala
425 430
taaccatcat gtccgatgca 1432
<210>138
<211>434
<212>PRT
<213>谷氨酸棒状杆菌
<400>138
Met Gly Lys His Glu Val Ala Gln Gln Thr Val Pro Gly Pro Ser Pro
1 5 10 15
Glu Met Glu Ala Gln Arg Arg Lys Glu Leu Arg Lys His Lys Ala Ile
20 25 30
Ala Thr Gly Leu Leu Ile Phe Ala Ala Ala Val Tyr Phe Leu Cys Arg
35 40 45
Phe Val Glu Thr Arg Pro Gly Glu Thr Ala Ala Trp Val Gly Phe Val
50 55 60
Arg Ala Ala Ala Glu Ala Gly Met Ile Gly Gly Leu Ala Asp Trp Phe
65 70 75 80
Ala Val Thr Ala Leu Phe Arg His Pro Leu Trp Leu Pro Ile Pro His
85 90 95
Thr Ala Ile Ile Pro Arg Lys Lys Asp Gln Leu Gly Glu Ala Leu Ser
100 105 110
Gly Phe Val Gly Asp Asn Phe Leu Asn Ala Gln Leu Ile Thr Glu Lys
115 120 125
Val Ser Gln Ala Arg Ile Pro Glu Arg Ala Gly Glu Trp Leu Ala Gln
130 135 140
Pro Glu Asn Gly Glu Lys Val Ser Arg Glu Val Gly Lys Leu Thr Ala
145 150 155 160
Asn Ile Val Arg Ala Ile Asp Pro Ser Asp Ala Glu Ala Val Ile Lys
165 170 175
Ser Ala Val Ile Asp Lys Leu Ala Glu Pro Thr Trp Gly Pro Pro Ala
180 185 190
Gly Arg Leu Leu Glu Gln Leu Leu Ala Glu Gly Lys Ala Glu Pro Val
195 200 205
Val Gln Glu Leu Ala Gln Trp Leu His Lys Lys Ala Leu Gly Ser Glu
210 215 220
Pro Leu Ile Asp Arg Leu Leu Asn Glu Arg Arg Pro Ile Trp Ala Pro
225 230 235 240
Lys Phe Thr Ala Gln Leu Val Ser Gly Lys Val Tyr Asp Glu Val Ile
245 250 255
Lys Phe Thr Glu Ala Val Ala Ala Asp Pro Asn His Glu Ala Arg Lys
260 265 270
Ser Leu Arg Arg Phe Leu Asn Lys Leu Ala Gln Asp Leu Gln His Asp
275 280 285
Pro Gly Met Ile Ile Lys Val Glu Glu Ile Lys Arg Asp Ile Met Gly
290 295 300
Ser Gly Ala Ile Ala Gln Ala Ala Pro Thr Ile Trp Ala Ser Ala Ser
305 310 315 320
Glu Ser Leu Ile Glu Ser Ala Glu Asp Glu Ser Ser Ile Leu Arg Arg
325 330 335
Lys Ile Ala Glu Ala Ala Thr Ser Trp Gly Gln Arg Leu Leu Val Asp
340 345 350
Asp Ser Leu Arg His Ser Leu Asp Thr Arg Ile Thr Gly Ala Ala Ala
355 360 365
Phe Leu Ala Asp Asn Tyr Ala Pro Glu Val Thr Gly Ile Ile Ser Glu
370 375 380
Thr Ile Glu Arg Trp Asp Ala Glu Glu Ala Ser Glu Lys Ile Glu Leu
385 390 395 400
Met Val Gly Lys Asp Leu Gln Tyr Ile Arg Leu Asn Gly Thr Ile Val
405 410 415
Gly Ala Leu Ala Gly Leu Ala Ile Tyr Ala Ile Ser His Ile Leu Phe
420 425 430
Gly Ala
<210>139
<211>496
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(466)
<223>RXA01326
<400>139
gtccttgagg ctgcttacac tctacttatt gtagaaatgg gagggtcgtg gcgtcgaaaa 60
gcaaacatct tttaacgctt ttggcccgta ttatggtggc atg gct ttc ccc gtg 115
Met Ala Phe Pro Val
1 5
aca gaa gac aaa atc ctg gca gct gag gaa acc ctt ggc agg cgc ctc 163
Thr Glu Asp Lys Ile Leu Ala Ala Glu Glu Thr Leu Gly Arg Arg Leu
10 15 20
ccc gaa act ttg cgc gaa cga cta ctt caa aac aat ggt ggc gaa gtc 211
Pro Glu Thr Leu Arg Glu Arg Leu Leu Gln Asn Asn Gly Gly Glu Val
25 30 35
atc gac aat gaa aac aac gac tgg att ctc cat cca gtt cgt gat gac 259
Ile Asp Asn Glu Asn Asn Asp Trp Ile Leu His Pro Val Arg Asp Asp
40 45 50
agc gac cga aaa agg ctt gtc cga act gcc aac gac atc atc cgc gag 307
Ser Asp Arg Lys Arg Leu Val Arg Thr Ala Asn Asp Ile Ile Arg Glu
55 60 65
acc gaa tct gca cgt gaa tgg gac aat ttc ccc gaa aat gcg atc gca 355
Thr Glu Ser Ala Arg Glu Trp Asp Asn Phe Pro Glu Asn Ala Ile Ala
70 75 80 85
att gca aat gac gga acg ggc gac tta ata att ctg ctt ccc gac gat 403
Ile Ala Asn Asp Gly Thr Gly Asp Leu Ile Ile Leu Leu Pro Asp Asp
90 95 100
gat gct ttc tac atc tgg tcg cac gaa gat gaa ccc ctg atc gaa act 451
Asp Ala Phe Tyr Ile Trp Ser His Glu Asp Glu Pro Leu Ile Glu Thr
105 110 115
gaa ctt gag gat gcc taaaacagct ggacagaatt cgacttggaa 496
Glu Leu Glu Asp Ala
120
<210>140
<211>122
<212>PRT
<213>谷氨酸棒状杆菌
<400>140
Met Ala Phe Pro Val Thr Glu Asp Lys Ile Leu Ala Ala Glu Glu Thr
1 5 10 15
Leu Gly Arg Arg Leu Pro Glu Thr Leu Arg Glu Arg Leu Leu Gln Asn
20 25 30
Asn Gly Gly Glu Val Ile Asp Asn Glu Asn Asn Asp Trp Ile Leu His
35 40 45
Pro Val Arg Asp Asp Ser Asp Arg Lys Arg Leu Val Arg Thr Ala Asn
50 55 60
Asp Ile Ile Arg Glu Thr Glu Ser Ala Arg Glu Trp Asp Asn Phe Pro
65 70 75 80
Glu Asn Ala Ile Ala Ile Ala Asn Asp Gly Thr Gly Asp Leu Ile Ile
85 90 95
Leu Leu Pro Asp Asp Asp Ala Phe Tyr Ile Trp Ser His Glu Asp Glu
100 105 110
Pro Leu Ile Glu Thr Glu Leu Glu Asp Ala
115 120
<210>141
<211>2527
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2497)
<223>RXA01331
<400>141
ctaaactcgt ctctcgtatc tttcagtcat ttatgtctga taagcaggcg cgtgcgccac 60
acaattggca caatcacaag aaagtgaagt gggaacctag atg ctg acg aca cta 115
Met Leu Thr Thr Leu
1 5
tgg att gcg gtg ttg gta ttt acc gtt cca gga ttg gtc gtc tcg tgg 163
Trp Ile Ala Val Leu Val Phe Thr Val Pro Gly Leu Val Val Ser Trp
10 15 20
gtt tct ggc crt aaa gtg ccc tgg gct atc gca gcc tcc arc cca gcc 211
Val Ser Gly Leu Lys Val Pro Trp Ala Ile Ala Ala Ser Ile Pro Ala
25 30 35
acc ttc ggt att tac ggc ctg tcc gcc tgg ttg ctg ggc ttg tgg gag 259
Thr Phe Gly Ile Tyr Gly Leu Ser Ala Trp Leu Leu Gly Leu Trp Glu
40 45 50
atg cgt ttt gat ctc cat tct gta gtt att tcc aca ttg gtt ttc gct 307
Met Arg Phe Asp Leu His Ser Val Val Ile Ser Thr Leu Val Phe Ala
55 60 65
gcg gtt gct ttg gta tgg cgc ttg ttt ttt gtc ggt ggt tgg ctt gta 355
Ala Val Ala Leu Val Trp Arg Leu Phe Phe Val Gly Gly Trp Leu Val
70 75 80 85
cgt cgg cgt aaa gca cgt atc cgc agg cag acg ctc gcg gat gaa gag 403
Arg Arg Arg Lys Ala Arg Ile Arg Arg Gln Thr Leu Ala Asp Glu Glu
90 95 100
cgg gca gaa aat gct gag gta tct gca ggg gag cct gcc gaa tcg agc 451
Arg Ala Glu Asn Ala Glu Val Ser Ala Gly Glu Pro Ala Glu Ser Ser
105 110 115
aca aac gaa gca gcc gaa tct gaa tct gaa acc tcg gag cgt cgc gga 499
Thr Asn Glu Ala Ala Glu Ser Glu Ser Glu Thr Ser Glu Arg Arg Gly
120 125 130
atc tgg cgc gtg atc ttt gat tac atg cgc gac ggt ggc atc ttg gat 547
Ile Trp Arg Val Ile Phe Asp Tyr Met Arg Asp Gly Gly Ile Leu Asp
135 140 145
cac cgt tgg ctg ctg cct gcc gca ggt gct atc act ggt gcg tgg ctg 595
His Arg Trp Leu Leu Pro Ala Ala Gly Ala Ile Thr Gly Ala Trp Leu
150 155 160 165
atc att gat cgt gcc gtt gat ctg ctc ttg agc acc gag cat ggt ttg 643
Ile Ile Asp Arg Ala Val Asp Leu Leu Leu Ser Thr Glu His Gly Leu
170 175 180
ggc gat atc gtc caa ggc tgg gat gtc cat tgg cat gct tcg act gtc 691
Gly Asp Ile Val Gln Gly Trp Asp Val His Trp His Ala Ser Thr Val
185 190 195
cgt ttt ata gat gag acc ggc att gcg tca tcc acg atg atg ggg cag 739
Arg Phe Ile Asp Glu Thr Gly Ile Ala Ser Ser Thr Met Met Gly Gln
200 205 210
ctg cgc aat att gaa acg cag caa gat ctg ttc tac cca agc gca tgg 787
Leu Arg Asn Ile Glu Thr Gln Gln Asp Leu Phe Tyr Pro Ser Ala Trp
215 220 225
cat gct ggt gca tgg gtg ctg tcg gat gtc gga aat ctg acg att gtt 835
His Ala Gly Ala Trp Val Leu Ser Asp Val Gly Asn Leu Thr Ile Val
230 235 240 245
gaa gcc acc aac ctc act ggc att gtg ctg tcc gga ttg ttg ctg ccg 883
Glu Ala Thr Asn Leu Thr Gly Ile Val Leu Ser Gly Leu Leu Leu Pro
250 255 260
tta gct gtt gca ctg att gca tgg cgg atg atc aac aat cgt gga ctg 931
Leu Ala Val Ala Leu Ile Ala Trp Arg Met Ile Asn Asn Arg Gly Leu
265 270 275
acc gcg cag att ggt gcg ggc ttt gct gga ctg atc acc att gcc tot 979
Thr Ala Gln Ile Gly Ala Gly Phe Ala Gly Leu Ile Thr Ile Ala Ser
280 285 290
ccg gta ctg ttc tgg gtt ggt aac tac gtg ggt gcg tgg cct tat gtt 1027
Pro Val Leu Phe Trp Val Gly Asn Tyr Val Gly Ala Trp Pro Tyr Val
295 300 305
gct gcg atc ggt gct tca ggt gtg gtg ctt gcg ctg ttt atg tcc act 1075
Ala Ala Ile Gly Ala Ser Gly Val Val Leu Ala Leu Phe Met Ser Thr
310 315 320 325
ccg tct gtg ccg gta aga atc ttt gcc gca gca ttg gcg ttc atg ggt 1123
Pro Ser Val Pro Val Arg Ile Phe Ala Ala Ala Leu Ala Phe Met Gly
330 335 340
atg ttc cag ctg cat cca gcc cca tcc acc atc gtg atc atg gtg ttg 1171
Met Phe Gln Leu His Pro Ala Pro Ser Thr Ile Val Ile Met Val Leu
345 350 355
ctg ctg tgg tgg ctg ctc aaa ctc gtg gtg gtt cca agc cag aaa gtg 1219
Leu Leu Trp Trp Leu Leu Lys Leu Val Val Val Pro Ser Gln Lys Val
360 365 370
aag ggc tgg aag gcg ggc atc ggt atc cgt ttg aag gat gtc ggc atc 1267
Lys Gly Trp Lys Ala Gly Ile Gly Ile Arg Leu Lys Asp Val Gly Ile
375 380 385
ctg gcc atc acg ggc atc atc ggt gtg ctc ttc atg ctg cct cag gtg 1315
Leu Ala Ile Thr Gly Ile Ile Gly Val Leu Phe Met Leu Pro Gln Val
390 395 400 405
att tca ggt tcc gaa caa acc gaa gat gtg ctg tca tat tct gct gag 1363
Ile Ser Gly Ser Glu Gln Thr Glu Asp Val Leu Ser Tyr Ser Ala Glu
410 415 420
gaa caa gtc acc cgc agc gag tcc tgg ttg gtg tct att ttc atg gag 1411
Glu Gln Val Thr Arg Ser Glu Ser Trp Leu Val Ser Ile Phe Met Glu
425 430 435
acc cgc cat gtt gat ttc ttc gga aat att gac atc gtc cca gtg ctg 1459
Thr Arg His Val Asp Phe Phe Gly Asn Ile Asp Ile Val Pro Val Leu
440 445 450
gta ttc gca gca atc ggt ggc gtg gtt gct ttg gtg tgg cgc gga aac 1507
Val Phe Ala Ala Ile Gly Gly Val Val Ala Leu Val Trp Arg Gly Asn
455 460 465
ttg tgg gcg ccg gtg ttt tac ttc gcc agc gtt gcg ttg acc gct aac 1555
Leu Trp Ala Pro Val Phe Tyr Phe Ala Ser Val Ala Leu Thr Ala Asn
470 475 480 485
tcg ctg aag cct ttt gaa gag ccg tgg ggt gat tgg ctc aac atc gtg 1603
Ser Leu Lys Pro Phe Glu Glu Pro Trp Gly Asp Trp Leu Asn Ile Val
490 495 500
ggc ggt ctg cat tac tcc aca gga cac cgt ttg atc atg cct gtc gcc 1651
Gly Gly Leu His Tyr Ser Thr Gly His Arg Leu Ile Met Pro Val Ala
505 510 515
atg ttc act ttt gct gcc gca ggt atc ggc gct gcc gca gtg atc cgt 1699
Met Phe Thr Phe Ala Ala Ala Gly Ile Gly Ala Ala Ala Val Ile Arg
520 525 530
ttg atc tgc ttg gga cca ata aag aag ttc acc act gtt tcc ggt gtt 1747
Leu Ile Cys Leu Gly Pro Ile Lys Lys Phe Thr Thr Val Ser Gly Val
535 540 545
gtt tct gtg gtg atg gct ctt gtt gtg gct gtg cca ttg cag act tgg 1795
Val Ser Val Val Met Ala Leu Val Val Ala Val Pro Leu Gln Thr Trp
550 555 560 565
gcg aag gat ttt gta gag gaa gga tcc gaa acc aca atc ctt gcg cca 1843
Ala Lys Asp Phe Val Glu Glu Gly Ser Glu Thr Thr Ile Leu Ala Pro
570 575 580
cac aat gat gaa cgt atg gtg agc aac aac gac ttg gct gcc tgg gac 1891
His Asn Asp Glu Arg Met Val Ser Asn Asn Asp Leu Ala Ala Trp Asp
585 590 595
tgg tta atc caa cag cca ggt gga gct gac atg aac atc atg ggt gac 1939
Trp Leu Ile Gln Gln Pro Gly Gly Ala Asp Met Asn Ile Met Gly Asp
600 605 610
ccc gca gat ggt aac ggc tgg atg tat gcc tac aac ggc ttg cac tcc 1987
Pro Ala Asp Gly Asn Gly Trp Met Tyr Ala Tyr Asn Gly Leu His Ser
615 620 625
gtg gcc cgc cac tat gca tgg cca gca gca ggc gaa ggc tct gcc acc 2035
Val Ala Arg His Tyr Ala Trp Pro Ala Ala Gly Glu Gly Ser Ala Thr
630 635 640 645
gcg atg ctg ttc tgg tgg cct caa ctt cta ggt gtg ggc acc gat gaa 2083
Ala Met Leu Phe Trp Trp Pro Gln Leu Leu Gly Val Gly Thr Asp Glu
650 655 660
aac cca gat caa gtc aac gat gtg gat cag gct gct cgt gat ctc aac 2131
Asn Pro Asp Gln Val Asn Asp Val Asp Gln Ala Ala Arg Asp Leu Asn
665 670 675
gtc ggc tac ttc atg atc agt ccg tgg acg ttc tgg gat ttc cag atc 2179
Val Gly Tyr Phe Met Ile Ser Pro Trp Thr Phe Trp Asp Phe Gln Ile
680 685 690
ccc aac ttc cgc cag atc gat ctg ctg tgg caa acc cca ggc gtg aca 2227
Pro Asn Phe Arg Gln Ile Asp Leu Leu Trp Gln Thr Pro Gly Val Thr
695 700 705
ccg gtg tac aag aag ggc gac tcg gtg atc ttc gca gtc aac gat atg 2275
Pro Val Tyr Lys Lys Gly Asp Ser Val Ile Phe Ala Val Asn Asp Met
710 715 720 725
ttc act gac gcc gaa ctg gat cag atg cgt gca cct ggt aat tct cca 2323
Phe Thr Asp Ala Glu Leu Asp Gln Met Arg Ala Pro Gly Asn Ser Pro
730 735 740
gaa cca ctg cca gag ctt cct acc ttg ggc gag ctt ggg ttg gct gaa 2371
Glu Pro Leu Pro Glu Leu Pro Thr Leu Gly Glu Leu Gly Leu Ala Glu
745 750 755
act gaa gac gag gta gat cag act tat tac cat cgt cca acg gtt cct 2419
Thr Glu Asp Glu Val Asp Gln Thr Tyr Tyr His Arg Pro Thr Val Pro
760 765 770
gct ggt gtg aac tca gag atg cct tca gcc gaa act ctg tat gca ccg 2467
Ala Gly Val Asn Ser Glu Met Pro Ser Ala Glu Thr Leu Tyr Ala Pro
775 780 785
gat cca acg aag ccg cat acg gtc cct aac taatcacaaa gagtattcag 2517
Asp Pro Thr Lys Pro His Thr Val Pro Asn
790 795
taaatcgacc 2527
<210>142
<211>799
<212>PRT
<213>谷氨酸棒状杆菌
<400>142
Met Leu Thr Thr Leu Trp Ile Ala Val Leu Val Phe Thr Val Pro Gly
1 5 10 15
Leu Val Val Ser Trp Val Ser Gly Leu Lys Val Pro Trp Ala Ile Ala
20 25 30
Ala Ser Ile Pro Ala Thr Phe Gly Ile Tyr Gly Leu Ser Ala Trp Leu
35 40 45
Leu Gly Leu Trp Glu Met Arg Phe Asp Leu His Ser Val Val Ile Ser
50 55 60
Thr Leu Val Phe Ala Ala Val Ala Leu Val Trp Arg Leu Phe Phe Val
65 70 75 80
Gly Gly Trp Leu Val Arg Arg Arg Lys Ala Arg Ile Arg Arg Gln Thr
85 90 95
Leu Ala Asp Glu Glu Arg Ala Glu Asn Ala Glu Val Ser Ala Gly Glu
100 105 110
Pro Ala Glu Ser Ser Thr Asn Glu Ala Ala Glu Ser Glu Ser Glu Thr
115 120 125
Ser Glu Arg Arg Gly Ile Trp Arg Val Ile Phe Asp Tyr Met Arg Asp
130 135 140
Gly Gly Ile Leu Asp His Arg Trp Leu Leu Pro Ala Ala Gly Ala Ile
145 150 155 160
Thr Gly Ala Trp Leu Ile Ile Asp Arg Ala Val Asp Leu Leu Leu Ser
165 170 175
Thr Glu His Gly Leu Gly Asp Ile Val Gln Gly Trp Asp Val His Trp
180 185 190
His Ala Ser Thr Val Arg Phe Ile Asp Glu Thr Gly Ile Ala Ser Ser
195 200 205
Thr Met Met Gly Gln Leu Arg Asn Ile Glu Thr Gln Gln Asp Leu Phe
210 215 220
Tyr Pro Ser Ala Trp His Ala Gly Ala Trp Val Leu Ser Asp Val Gly
225 230 235 240
Asn Leu Thr Ile Val Glu Ala Thr Asn Leu Thr Gly Ile Val Leu Ser
245 250 255
Gly Leu Leu Leu Pro Leu Ala Val Ala Leu Ile Ala Trp Arg Met Ile
260 265 270
Asn Asn Arg Gly Leu Thr Ala Gln Ile Gly Ala Gly Phe Ala Gly Leu
275 280 285
Ile Thr Ile Ala Ser Pro Val Leu Phe Trp Val Gly Asn Tyr Val Gly
290 295 300
Ala Trp Pro Tyr Val Ala Ala Ile Gly Ala Ser Gly Val Val Leu Ala
305 310 315 320
Leu Phe Met Ser Thr Pro Ser Val Pro Val Arg Ile Phe Ala Ala Ala
325 330 335
Leu Ala Phe Met Gly Met Phe Gln Leu His Pro Ala Pro Ser Thr Ile
340 345 350
Val Ile Met Val Leu Leu Leu Trp Trp Leu Leu Lys Leu Val Val Val
355 360 365
Pro Ser Gln Lys Val Lys Gly Trp Lys Ala Gly Ile Gly Ile Arg Leu
370 375 380
Lys Asp Val Gly Ile Leu Ala Ile Thr Gly Ile Ile Gly Val Leu Phe
385 390 395 400
Met Leu Pro Gln Val Ile Ser Gly Ser Glu Gln Thr Glu Asp Val Leu
405 410 415
Ser Tyr Ser Ala Glu Glu Gln Val Thr Arg Ser Glu Ser Trp Leu Val
420 425 430
Ser Ile Phe Met Glu Thr Arg His Val Asp Phe Phe Gly Asn Ile Asp
435 440 445
Ile Val Pro Val Leu Val Phe Ala Ala Ile Gly Gly Val Val Ala Leu
450 455 460
Val Trp Arg Gly Asn Leu Trp Ala Pro Val Phe Tyr Phe Ala Ser Val
465 470 475 480
Ala Leu Thr Ala Asn Ser Leu Lys Pro Phe Glu Glu Pro Trp Gly Asp
485 490 495
Trp Leu Asn Ile Val Gly Gly Leu His Tyr Ser Thr Gly His Arg Leu
500 505 510
Ile Met Pro Val Ala Met Phe Thr Phe Ala Ala Ala Gly Ile Gly Ala
515 520 525
Ala Ala Val Ile Arg Leu Ile Cys Leu Gly Pro Ile Lys Lys Phe Thr
530 535 540
Thr Val Ser Gly Val Val Ser Val Val Met Ala Leu Val Val Ala Val
545 550 555 560
Pro Leu Gln Thr Trp Ala Lys Asp Phe Val Glu Glu Gly Ser Glu Thr
565 570 575
Thr Ile Leu Ala Pro His Asn Asp Glu Arg Met Val Ser Asn Asn Asp
580 585 590
Leu Ala Ala Trp Asp Trp Leu Ile Gln Gln Pro Gly Gly Ala Asp Met
595 600 605
Asn Ile Met Gly Asp Pro Ala Asp Gly Asn Gly Trp Met Tyr Ala Tyr
610 615 620
Asn Gly Leu His Ser Val Ala Arg His Tyr Ala Trp Pro Ala Ala Gly
625 630 635 640
Glu Gly Ser Ala Thr Ala Met Leu Phe Trp Trp Pro Gln Leu Leu Gly
645 650 655
Val Gly Thr Asp Glu Asn Pro Asp Gln Val Asn Asp Val Asp Gln Ala
660 665 670
Ala Arg Asp Leu Asn Val Gly Tyr Phe Met Ile Ser Pro Trp Thr Phe
675 680 685
Trp Asp Phe Gln Ile Pro Asn Phe Arg Gln Ile Asp Leu Leu Trp Gln
690 695 700
Thr Pro Gly Val Thr Pro Val Tyr Lys Lys Gly Asp Ser Val Ile Phe
705 710 715 720
Ala Val Asn Asp Met Phe Thr Asp Ala Glu Leu Asp Gln Met Arg Ala
725 730 735
Pro Gly Asn Ser Pro Glu Pro Leu Pro Glu Leu Pro Thr Leu Gly Glu
740 745 750
Leu Gly Leu Ala Glu Thr Glu Asp Glu Val Asp Gln Thr Tyr Tyr His
755 760 765
Arg Pro Thr Val Pro Ala Gly Val Asn Ser Glu Met Pro Ser Ala Glu
770 775 780
Thr Leu Tyr Ala Pro Asp Pro Thr Lys Pro His Thr Val Pro Asn
785 790 795
<210>143
<211>1777
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1747)
<223>RXA01400
<400>143
cgccggggac ggggaaggcg ctgtgttgta ggggagagtt catggaaact tcctaaatta 60
ttttctatca atctatagat actaggtcta ttatagtgcc atg aga ctg aca cat 115
Met Arg Leu Thr His
1 5
gta gac caa tta aac ctt ccg ttt ggc acg ttg agc agc tat cac cct 163
Val Asp Gln Leu Asn Leu Pro Phe Gly Thr Leu Ser Ser Tyr His Pro
10 15 20
tat gca tcg gct ccg ggt gag gcg gtg ccg gtg tcc ttt gat cag caa 211
Tyr Ala Ser Ala Pro Gly Glu Ala Val Pro Val Ser Phe Asp Gln Gln
25 30 35
cga cat gtg ggt gcc ggc gag cga ccg ggt agt tgg atg gcg att agt 259
Arg His Val Gly Ala Gly Glu Arg Pro Gly Ser Trp Met Ala Ile Ser
40 45 50
gtt tct atg cct aag gcg atc agc cgt gag gaa ttg gag cgc gcg tgg 307
Val Ser Met Pro Lys Ala Ile Ser Arg Glu Glu Leu Glu Arg Ala Trp
55 60 65
ctt gaa gtg att gaa cgg cac ggc acc ttg cgc aca gta ttt agc acg 355
Leu Glu Val Ile Glu Arg His Gly Thr Leu Arg Thr Val Phe Ser Thr
70 75 80 85
gga atg ggt ggg gaa gtg cag caa cac cgc att gat gtg ggc ccg gga 403
Gly Met Gly Gly Glu Val Gln Gln His Arg Ile Asp Val Gly Pro Gly
90 95 100
aaa tgg att gac cac gcc gtt gcg cct ggt gag agc att aat gag gcg 451
Lys Trp Ile Asp His Ala Val Ala Pro Gly Glu Ser Ile Asn Glu Ala
105 110 115
ttg cgg gcg gtg ttg aat cgg cag tgt tcg ccg tac tcg agg cca tcg 499
Leu Arg Ala Val Leu Asn Arg Gln Cys Ser Pro Tyr Ser Arg Pro Ser
120 125 130
cat agt ttg tgc att att gat gcg cat ccg cgt ccc acg gtg att att 547
His Ser Leu Cys Ile Ile Asp Ala His Pro Arg Pro Thr Val Ile Ile
135 140 145
ggt agt gat cat tcg cat gtg gat atg tgg tcc atg ctg gtg att gtg 595
Gly Ser Asp His Ser His Val Asp Met Trp Ser Met Leu Val Ile Val
150 155 160 165
cgc gat ttg ttg gct gcg ctc gat atg gaa ctt ccc gtt gag ccg ccg 643
Arg Asp Leu Leu Ala Ala Leu Asp Met Glu Leu Pro Val Glu Pro Pro
170 175 180
ttg gcg ttt gaa tcg cac acc gcg gaa ctc ctc gcg gct cca cct gca 691
Leu Ala Phe Glu Ser His Thr Ala Glu Leu Leu Ala Ala Pro Pro Ala
185 190 195
ccg gag cgg att cac cag cgg tgg cgt gaa att ttg gaa gct ggt ggt 739
Pro Glu Arg Ile His Gln Arg Trp Arg Glu Ile Leu Glu Ala Gly Gly
200 205 210
gga aaa atg cct cag ttc ccg ctt ccg ctt ggc gat gcc atc tcc atg 787
Gly Lys Met Pro Gln Phe Pro Leu Pro Leu Gly Asp Ala Ile Ser Met
215 220 225
cct gaa cgc gtt gaa gtc cgc gac att ttc ggg gtc aat ggc ttg gcg 835
Pro Glu Arg Val Glu Val Arg Asp Ile Phe Gly Val Asn Gly Leu Ala
230 235 240 245
att tac tcg gca cgc gcc cgc gca caa cag gta agc tcc ctg gcc ttg 883
Ile Tyr Ser Ala Arg Ala Arg Ala Gln Gln Val Ser Ser Leu Ala Leu
250 255 260
act att tca gtg atg gct gat gtt acg gcg gcg ctg gcg gac ctt ccg 931
Thr Ile Ser Val Met Ala Asp Val Thr Ala Ala Leu Ala Asp Leu Pro
265 270 275
ttg cgt gct gta ttc cca gtc cat agc cgt ttc gat cag cgc tgg cac 979
Leu Arg Ala Val Phe Pro Val His Ser Arg Phe Asp Gln Arg Trp His
280 285 290
gat agt gtc ggg tgg ttt atc tcc aat tcg gtc att gag gtg cca gat 1027
Asp Ser Val Gly Trp Phe Ile Ser Asn Ser Val Ile Glu Val Pro Asp
295 300 305
tcc gat cct cac act gca gcc caa gct gtt cgc gag gct gtt tct ttg 1075
Ser Asp Pro His Thr Ala Ala Gln Ala Val Arg Glu Ala Val Ser Leu
310 315 320 325
gga agc tac ccg ctg gct gag ctg ctt gaa ccg tgg ggt ggc atg ccg 1123
Gly Ser Tyr Pro Leu Ala Glu Leu Leu Glu Pro Trp Gly Gly Met Pro
330 335 340
gaa acg cca gga atg ttt gct att tct tgg ctt gac ctg cgc cga ctc 1171
Glu Thr Pro Gly Met Phe Ala Ile Ser Trp Leu Asp Leu Arg Arg Leu
345 350 355
cca gtg agc att gac gat att ggc ctc caa gcc cag tat gtc agc gct 1219
Pro Val Ser Ile Asp Asp Ile Gly Leu Gln Ala Gln Tyr Val Ser Ala
360 365 370
tca ctg cgc acc gat ggt gtg atg ctg tgg ttt att ttg gat cgc tcc 1267
Ser Leu Arg Thr Asp Gly Val Met Leu Trp Phe Ile Leu Asp Arg Ser
375 380 385
ggc gcg cac ctt cgc tgt cgt tat cct gac tct ttg gtg gcg cgg gaa 1315
Gly Ala His Leu Arg Cys Arg Tyr Pro Asp Ser Leu Val Ala Arg Glu
390 395 400 405
aat gta ggc cgc tgg att gat gcg att gtt gct cag atg cgc gcc gaa 1363
Asn Val Gly Arg Trp Ile Asp Ala Ile Val Ala Gln Met Arg Ala Glu
410 415 420
gct ggg acg gtg aat ctg cag gcc ggc ggg gaa cag ctg aca ctt cgg 1411
Ala Gly Thr Val Asn Leu Gln Ala Gly Gly Glu Gln Leu Thr Leu Arg
425 430 435
cat gga act cgc gcc gat att tcc gag atc gcc ccg cta ctt gcc cga 1459
His Gly Thr Arg Ala Asp Ile Ser Glu Ile Ala Pro Leu Leu Ala Arg
440 445 450
aac gca gct gac cct tgt gag ctg gtg gat ctt gaa cat gcc ctt gac 1507
Asr Ala Ala Asp Pro Cys Glu Leu Val Asp Leu Glu His Ala Leu Asp
455 460 465
ctg ctg aca cac gag tct tcg cat ttc ctg gcg gtg gtt cga aac gct 1555
Leu Leu Thr His Glu Ser Ser His Phe Leu Ala Val Val Arg Asn Ala
470 475 480 485
cgt ggc aag ata atc gca gcg atg cag ttg act att gtt ccg gag ttt 1603
Arg Gly Lys Ile Ile Ala Ala Met Gln Leu Thr Ile Val Pro Glu Phe
490 495 500
tcc cgc ggt ggt gcg ctt cat ctt cac atc gag ggg ccg ttc att att 1651
Ser Arg Gly Gly Ala Leu His Leu His Ile Glu Gly Pro Phe Ile Ile
505 510 515
ccg gaa tat cgc acc acc gat ttg gat aaa aag ctg cgc gcc tgg gct 1699
Pro Glu Tyr Arg Thr Thr Asp Leu Asp Lys Lys Leu Arg Ala Trp Ala
520 525 530
gtg gag cat ggg cgt gcg cga ggg gtg aag gtt gag gag gtg gtg gga 1747
Val Glu His Gly Arg Ala Arg Gly Val Lys Val Glu Glu Val Val Gly
535 540 545
tagttggttt attggcgcct cgttgtggaa 1777
<210>144
<211>549
<212>PRT
<213>谷氨酸棒状杆菌
<400>144
Met Arg Leu Thr His Val Asp Gln Leu Asn Leu Pro Phe Gly Thr Leu
1 5 10 15
Ser Ser Tyr His Pro Tyr Ala Ser Ala Pro Gly Glu Ala Val Pro Val
20 25 30
Ser Phe Asp Gln Gln Arg His Val Gly Ala Gly Glu Arg Pro Gly Ser
35 40 45
Trp Met Ala Ile Ser Val Ser Met Pro Lys Ala Ile Ser Arg Glu Glu
50 55 60
Leu Glu Arg Ala Trp Leu Glu Val Ile Glu Arg His Gly Thr Leu Arg
65 70 75 80
Thr Val Phe Ser Thr Gly Met Gly Gly Glu Val Gln Gln His Arg Ile
85 90 95
Asp Val Gly Pro Gly Lys Trp Ile Asp His Ala Val Ala Pro Gly Glu
100 105 110
Ser Ile Asn Glu Ala Leu Arg Ala Val Leu Asn Arg Gln Cys Ser Pro
115 120 125
Tyr Ser Arg Pro Ser His Ser Leu Cys Ile Ile Asp Ala His Pro Arg
130 135 140
Pro Thr Val Ile Ile Gly Ser Asp His Ser His Val Asp Met Trp Ser
145 150 155 160
Met Leu Val Ile Val Arg Asp Leu Leu Ala Ala Leu Asp Met Glu Leu
165 170 175
Pro Val Glu Pro Pro Leu Ala Phe Glu Ser His Thr Ala Glu Leu Leu
180 185 190
Ala Ala Pro Pro Ala Pro Glu Arg Ile His Gln Arg Trp Arg Glu Ile
195 200 205
Leu Glu Ala Gly Gly Gly Lys Met Pro Gln Phe Pro Leu Pro Leu Gly
210 215 220
Asp Ala Ile Ser Met Pro Glu Arg Val Glu Val Arg Asp Ile Phe Gly
225 230 235 240
Val Asn Gly Leu Ala Ile Tyr Ser Ala Arg Ala Arg Ala Gln Gln Val
245 250 255
Ser Ser Leu Ala Leu Thr Ile Ser Val Met Ala Asp Val Thr Ala Ala
260 265 270
Leu Ala Asp Leu Pro Leu Arg Ala Val Phe Pro Val His Ser Arg Phe
275 280 285
Asp Gln Arg Trp His Asp Ser Val Gly Trp Phe Ile Ser Asn Ser Val
290 295 300
Ile Glu Val Pro Asp Ser Asp Pro His Thr Ala Ala Gln Ala Val Arg
305 310 315 320
Glu Ala Val Ser Leu Gly Ser Tyr Pro Leu Ala Glu Leu Leu Glu Pro
325 330 335
Trp Gly Gly Met Pro Glu Thr Pro Gly Met Phe Ala Ile Ser Trp Leu
340 345 350
Asp Leu Arg Arg Leu Pro Val Ser Ile Asp Asp Ile Gly Leu Gln Ala
355 360 365
Gln Tyr Val Ser Ala Ser Leu Arg Thr Asp Gly Val Met Leu Trp Phe
370 375 380
Ile Leu Asp Arg Ser Gly Ala His Leu Arg Cys Arg Tyr Pro Asp Ser
385 390 395 400
Leu Val Ala Arg Glu Asn Val Gly Arg Trp Ile Asp Ala Ile Val Ala
405 410 415
Gln Met Arg Ala Glu Ala Gly Thr Val Asn Leu Gln Ala Gly Gly Glu
420 425 430
Gln Leu Thr Leu Arg His Gly Thr Arg Ala Asp Ile Ser Glu Ile Ala
435 440 445
Pro Leu Leu Ala Arg Asn Ala Ala Asp Pro Cys Glu Leu Val Asp Leu
450 455 460
Glu His Ala Leu Asp Leu Leu Thr His Glu Ser Ser His Phe Leu Ala
465 470 475 480
Val Val Arg Asn Ala Arg Gly Lys Ile Ile Ala Ala Met Gln Leu Thr
485 490 495
Ile Val Pro Glu Phe Ser Arg Gly Gly Ala Leu His Leu His Ile Glu
500 505 510
Gly Pro Phe Ile Ile Pro Glu Tyr Arg Thr Thr Asp Leu Asp Lys Lys
515 520 525
Leu Arg Ala Trp Ala Val Glu His Gly Arg Ala Arg Gly Val Lys Val
530 535 540
Glu Glu Val Val Gly
545
<210>145
<211>1081
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1051)
<223>RXA01425
<400>145
ccggtgccga accatggatt ttggtccacc gaaacggtga catagaagtc ctgccaacaa 60
gtacatagtc cctattaatc ccaaggagtt tcgactcaca gtg ctc aat ttc att 115
Val Leu Asn Phe Ile
1 5
tat tgg cca att tcg gcc att ctg tgg ttc tgg cat aaa gcg ttc agc 163
Tyr Trp Pro Ile Ser Ala Ile Leu Trp Phe Trp His Lys Ala Phe Ser
10 15 20
ttt gtg ctg agc cca gat tcc gga att acc tgg gcc ttg tcg atc atg 211
Phe Val Leu Ser Pro Asp Ser Gly Ile Thr Trp Ala Leu Ser Ile Met
25 30 35
ttc ttg acc ttc acc gtg cgt atg gtt ctg gtc aag ccg atg gtc aac 259
Phe Leu Thr Phe Thr Val Arg Met Val Leu Val Lys Pro Met Val Asn
40 45 50
acc atg cgt tca cag cgc aag atg caa gac atg gct cca aag atg cag 307
Thr Met Arg Ser Gln Arg Lys Met Gln Asp Met Ala Pro Lys Met Gln
55 60 65
gcc atc cgc gag aag tac aaa aat gac cag cag aag atg atg gag gag 355
Ala Ile Arg Glu Lys Tyr Lys Asn Asp Gln Gln Lys Met Met Glu Glu
70 75 80 85
acc cgc aaa ctt caa aaa gaa gtg ggc gtt aac ccc atc gca ggc tgt 403
Thr Arg Lys Leu Gln Lys Glu Val Gly Val Asn Pro Ile Ala Gly Cys
90 95 100
ttg cca atg ttg gtg cag atc cca gtg ttc ctg ggt ctg ttc cac gtg 451
Leu Pro Met Leu Val Gln Ile Pro Val Phe Leu Gly Leu Phe His Val
105 110 115
ctg cgc tcc ttc aac cgc acc ggt tct ggc gtt ggc cag ctg gaa atg 499
Leu Arg Ser Phe Asn Arg Thr Gly Ser Gly Val Gly Gln Leu Glu Met
120 125 130
acc gtt gag caa aac gcg aac acc ccg aac tac atc ttc ggt gtc gac 547
Thr Val Glu Gln Asn Ala Asn Thr Pro Asn Tyr Ile Phe Gly Val Asp
135 140 145
gag gtt cag tcc ttc ctg cgt gca gac ctg ttc ggt gcg cca ctg tcg 595
Glu Val Gln Ser Phe Leu Arg Ala Asp Leu Phe Gly Ala Pro Leu Ser
150 155 160 165
tcc tac atc acc atg cct gct gac gcg ttc gac gcg ttc ctt ggc ctg 643
Ser Tyr Ile Thr Met Pro Ala Asp Ala Phe Asp Ala Phe Leu Gly Leu
170 175 180
gat gtc tcc cgc ctc aac atc gcg ctg gtt gca gct cca atg att ttg 691
Asp Val Ser Arg Leu Asn Ile Ala Leu Val Ala Ala Pro Met Ile Leu
185 190 195
atc att gtc gtg gca act cac atg aac gcg cgt ctg tcc gtc aac cgc 739
Ile Ile Val Val Ala Thr His Met Asn Ala Arg Leu Ser Val Asn Arg
200 205 210
cag gaa gct cgc aag gca gcc ggc aag cag cag gcc gct tcc agc gat 787
Gln Glu Ala Arg Lys Ala Ala Gly Lys Gln Gln Ala Ala Ser Ser Asp
215 220 225
cag atg gcc atg cag atg caa atg atg aac aag atg atg ctc tgg ttc 835
Gln Met Ala Met Gln Met Gln Met Met Asn Lys Met Met Leu Trp Phe
230 235 240 245
atg cca gcc acc att ttg ttc acc ggc ttc atc tgg acc atc ggt ctt 883
Met Pro Ala Thr Ile Leu Phe Thr Gly Phe Ile Trp Thr Ile Gly Leu
250 255 260
ctt gtc tac atg atg tcc aac aac gtg tgg acc ttc ttc cag cag cgc 931
Leu Val Tyr Met Met Ser Asn Asn Val Trp Thr Phe Phe Gln Gln Arg
265 270 275
tac atc ttc gcc aag atg gac gct gag gaa gca gct gag gag gag gaa 979
Tyr Ile Phe Ala Lys Met Asp Ala Glu Glu Ala Ala Glu Glu Glu Glu
280 285 290
aag cgc gca gca aag cgc act acc gct cca aag cct ggc gtg aag cca 1027
Lys Arg Ala Ala Lys Arg Thr Thr Ala Pro Lys Pro Gly Val Lys Pro
295 300 305
gaa aac ccc aag aag cgt aag aag taaaacttca ctaaaaaccg ccagcccacg 1081
Glu Asn Pro Lys Lys Arg Lys Lys
310 315
<210>146
<211>317
<212>PRT
<213>谷氨酸棒状杆菌
<400>146
Val Leu Asn Phe Ile Tyr Trp Pro Ile Ser Ala Ile Leu Trp Phe Trp
1 5 10 15
His Lys Ala Phe Ser Phe Val Leu Ser Pro Asp Ser Gly Ile Thr Trp
20 25 30
Ala Leu Ser Ile Met Phe Leu Thr Phe Thr Val Arg Met Val Leu Val
35 40 45
Lys Pro Met Val Asn Thr Met Arg Ser Gln Arg Lys Met Gln Asp Met
50 55 60
Ala Pro Lys Met Gln Ala Ile Arg Glu Lys Tyr Lys Asn Asp Gln Gln
65 70 75 80
Lys Met Met Glu Glu Thr Arg Lys Leu Gln Lys Glu Val Gly Val Asn
85 90 95
Pro Ile Ala Gly Cys Leu Pro Met Leu Val Gln Ile Pro Val Phe Leu
100 105 110
Gly Leu Phe His Val Leu Arg Ser Phe Asn Arg Thr Gly Ser Gly Val
115 120 125
Gly Gln Leu Glu Met Thr Val Glu Gln Asn Ala Asn Thr Pro Asn Tyr
130 135 140
Ile Phe Gly Val Asp Glu Val Gln Ser Phe Leu Arg Ala Asp Leu Phe
145 150 155 160
Gly Ala Pro Leu Ser Ser Tyr Ile Thr Met Pro Ala Asp Ala Phe Asp
165 170 175
Ala Phe Leu Gly Leu Asp Val Ser Arg Leu Asn Ile Ala Leu Val Ala
180 185 190
Ala Pro Met Ile Leu Ile Ile Val Val Ala Thr His Met Asn Ala Arg
195 200 205
Leu Ser Val Asn Arg Gln Glu Ala Arg Lys Ala Ala Gly Lys Gln Gln
210 215 220
Ala Ala Ser Ser Asp Gln Met Ala Met Gln Met Gln Met Met Asn Lys
225 230 235 240
Met Met Leu Trp Phe Met Pro Ala Thr Ile Leu Phe Thr Gly Phe Ile
245 250 255
Trp Thr Ile Gly Leu Leu Val Tyr Met Met Ser Asn Asn Val Trp Thr
260 265 270
Phe Phe Gln Gln Arg Tyr Ile Phe Ala Lys Met Asp Ala Glu Glu Ala
275 280 285
Ala Glu Glu Glu Glu Lys Arg Ala Ala Lys Arg Thr Thr Ala Pro Lys
290 295 300
Pro Gly Val Lys Pro Glu Asn Pro Lys Lys Arg Lys Lys
305 310 315
<210>147
<211>3457
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3427)
<223>RXA01434
<400>147
acgggatcgc ccaaaccgcc accaacccac taacgtatcg catagttatt ttcgccgtgc 60
tcgccgttaa ggcgcgcact cgtacatgaa tggtcaacaa gtg agt tct tcg ctt 115
Val Ser Ser Ser Leu
1 5
tcg aat aat tcg gag cag tcc ggc ctg cgt ggc agg atc gtt gct cca 163
Ser Asn Asn Ser Glu Gln Ser Gly Leu Arg Gly Arg Ile Val Ala Pro
10 15 20
gca ccg ccg gcg cct gtg ccc gag gcg cgc aag aag gct gtc gca cgc 211
Ala Pro Pro Ala Pro Val Pro Glu Ala Arg Lys Lys Ala Val Ala Arg
25 30 35
acg gat ggt gat cgc tcg agt ttg aaa aac tcg cct acg gca tcc gcc 259
Thr Asp Gly Asp Arg Ser Ser Leu Lys Asn Ser Pro Thr Ala Ser Ala
40 45 50
acc cag gca gcc cag acg cgc ccg gca gaa ccg gaa cca aaa aag cac 307
Thr Gln Ala Ala Gln Thr Arg Pro Ala Glu Pro Glu Pro Lys Lys His
55 60 65
acc tcc gat tct gat gtg gtg cgc tcg act ggc tcc atg gca ata gcc 355
Thr Ser Asp Ser Asp Val Val Arg Ser Thr Gly Ser Met Ala Ile Ala
70 75 80 85
acg ctg ctg agt cgt atc acc ggt ttc ctg cgc acc gtg atg att ggt 403
Thr Leu Leu Ser Arg Ile Thr Gly Phe Leu Arg Thr Val Met Ile Gly
90 95 100
gcg gcg ctg tcg ccg gcc atc gct tcg gcg ttc aac act gcc aac acg 451
Ala Ala Leu Ser Pro Ala Ile Ala Ser Ala Phe Asn Thr Ala Asn Thr
105 110 115
ctg ccc aac ctg atc act gaa atc gtg ttg ggt gcg gtg ctg aca tcg 499
Leu Pro Asn Leu Ile Thr Glu Ile Val Leu Gly Ala Val Leu Thr Ser
120 125 130
ctg gtt att ccg gtc ctt acc cgc gcg gaa aaa gaa gac gcc gac ggc 547
Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys Glu Asp Ala Asp Gly
135 140 145
ggt tcc ggg ttc ttc agg cgg ctg ctc acc ctg tcg gtg acg ctg ctg 595
Gly Ser Gly Phe Phe Arg Arg Leu Leu Thr Leu Ser Val Thr Leu Leu
150 155 160 165
ggt ggt gtc acc atc ctg tcg att atc ggc gcg ccg ctg ctg aca cgg 643
Gly Gly Val Thr Ile Leu Ser Ile Ile Gly Ala Pro Leu Leu Thr Arg
170 175 180
atg atg ctg tcc tct gag gga caa gtc aac gtg gtc atg tcc acg gcc 691
Met Met Leu Ser Ser Glu Gly Gln Val Asn Val Val Met Ser Thr Ala
185 190 195
ttt gcg tat tgg ctg ctg cca cag att ttc ttc tac ggc ctg ttt gcc 739
Phe Ala Tyr Trp Leu Leu Pro Gln Ile Phe Phe Tyr Gly Leu Phe Ala
200 205 210
ctg ttc atg gct gtg ttg aac acc cgt gaa gtg ttc aaa ccc ggc gcg 787
Leu Phe Met Ala Val Leu Asn Thr Arg Glu Val Phe Lys Pro Gly Ala
215 220 225
tgg gca cct gtt gtc aac aat gtg atc acc ttg acc gtg ctg ggc gtg 835
Trp Ala Pro Val Val Asn Asn Val Ile Thr Leu Thr Val Leu Gly Val
230 235 240 245
tac atg gtg ctg cct gcg cgt ttg cac ccg cat gag cag gtg ggc att 883
Tyr Met Val Leu Pro Ala Arg Leu His Pro His Glu Gln Val Gly Ile
250 255 260
ttt gat ccg cag atc att ttc ctc ggc gtg ggc acc acc ctt ggt gtg 931
Phe Asp Pro Gln Ile Ile Phe Leu Gly Val Gly Thr Thr Leu Gly Val
265 270 275
gtt gca cag tgt cta atc atg att ccg tac ctg cgt cgc gcg ggc att 979
Val Ala Gln Cys Leu Ile Met Ile Pro Tyr Leu Arg Arg Ala Gly Ile
280 285 290
gat atg cgc cct ctg tgg ggt atc gat gcg cgt ttg aag caa ttc ggt 1027
Asp Met Arg Pro Leu Trp Gly Ile Asp Ala Arg Leu Lys Gln Phe Gly
295 300 305
ggc atg gcg atg gcg atc atc gtg tac gtg gca atc tcc cag ttc ggt 1075
Gly Met Ala Met Ala Ile Ile Val Tyr Val Ala Ile Ser Gln Phe Gly
310 315 320 325
tac atc atc acc act cgc att gcg tcg att gca gac gat gct gcg ccg 1123
Tyr Ile Ile Thr Thr Arg Ile Ala Ser Ile Ala Asp Asp Ala Ala Pro
330 335 340
ttt att tat cag cag cac tgg atg ttg ctg caa gtt cct tat ggc atc 1171
Phe Ile Tyr Gln Gln His Trp Met Leu Leu Gln Val Pro Tyr Gly Ile
345 350 355
atc ggc gtc acc ttg ctc acc gcg att atg ccg cga ctg tcc cgc aac 1219
Ile Gly Val Thr Leu Leu Thr Ala Ile Met Pro Arg Leu Ser Arg Asn
360 365 370
gcg gca gac ggc gat gat agg gca gta gtc tct gac ctt cag ttg ggt 1267
Ala Ala Asp Gly Asp Asp Arg Ala Val Val Ser Asp Leu Gln Leu Gly
375 380 385
tcc aag cta acc ttc atc gca ctg atc ccc atc gtg gtg ttc ttc acc 1315
Ser Lys Leu Thr Phe Ile Ala Leu Ile Pro Ile Val Val Phe Phe Thr
390 395 400 405
gcc ttc ggt gtc cct att gcc aat ggc ctt ttt gcc tac ggc caa ttc 1363
Ala Phe Gly Val Pro Ile Ala Asn Gly Leu Phe Ala Tyr Gly Gln Phe
410 415 420
gat gcc aac gcc gcc aac atc ctt ggt tgg act ctg agc ttc tct gct 1411
Asp Ala Asn Ala Ala Asn Ile Leu Gly Trp Thr Leu Ser Phe Ser Ala
425 430 435
ttc acg ctg att cct tac gct ttg gtg ctg cta cat ctg cgt gtg ttt 1459
Phe Thr Leu Ile Pro Tyr Ala Leu Val Leu Leu His Leu Arg Val Phe
440 445 450
tat gcg cgt gaa gag gtc tgg acc cca acc ttc atc atc gcc ggc atc 1507
Tyr Ala Arg Glu Glu Val Trp Thr Pro Thr Phe Ile Ile Ala Gly Ile
455 460 465
acc gcc acc aag gtc gtg ctt tcc ctg ttg gca ccg ctg ctg tcg agc 1555
Thr Ala Thr Lys Val Val Leu Ser Leu Leu Ala Pro Leu Leu Ser Ser
470 475 480 485
tcc ccg gag cgt gtg gtg gtg ctt ctt ggt gcg gcc aac ggt ttc agt 1603
Ser Pro Glu Arg Val Val Val Leu Leu Gly Ala Ala Asn Gly Phe Ser
490 495 500
ttc atc acc ggc gcg gtc atc ggc gcg tat ctg ttg cgc aac aaa ctc 1651
Phe Ile Thr Gly Ala Val Ile Gly Ala Tyr Leu Leu Arg Asn Lys Leu
505 510 515
ggc ctg ttg ggt atg cgc tct ttg gct aaa acc tcc ctg tgg gcg ttg 1699
Gly Leu Leu Gly Met Arg Ser Leu Ala Lys Thr Ser Leu Trp Ala Leu
520 525 530
ggc tct gcg gcg gtt ggt gca gca gca gca tgg gcg ttg ggg tgg ctg 1747
Gly Ser Ala Ala Val Gly Ala Ala Ala Ala Trp Ala Leu Gly Trp Leu
535 540 545
att caa gcc gtc gtg ggc gat ttc ttg ctg ggc act cta agc tcc gta 1795
Ile Gln Ala Val Val Gly Asp Phe Leu Leu Gly Thr Leu Ser Ser Val
550 555 560 565
ggc tac ttg ttg aac ctg gct gtg ttg ggt gtc ttc ttc atc ttc gtc 1843
Gly Tyr Leu Leu Asn Leu Ala Val Leu Gly Val Phe Phe Ile Phe Val
570 575 580
acc ggc atc gtg ttg tca cgt tct ggt ttg ccg gag gtc caa aac ttg 1891
Thr Gly Ile Val Leu Ser Arg Ser Gly Leu Pro Glu Val Gln Asn Leu
585 590 595
ggc cag gca ctg acc cgc atc cca ggt ttg agc cgg ttt att cgc ccg 1939
Gly Gln Ala Leu Thr Arg Ile Pro Gly Leu Ser Arg Phe Ile Arg Pro
600 605 610
aat acc aag atc tct ttg gat gtc ggc gaa gtc tcc gag cag gat ttc 1987
Asn Thr Lys Ile Ser Leu Asp Val Gly Glu Val Ser Glu Gln Asp Phe
615 620 625
tcc acc cag ctg gtc gcg cca agc gag ttc gca gca acc cct gtg ccg 2035
Ser Thr Gln Leu Val Ala Pro Ser Glu Phe Ala Ala Thr Pro Val Pro
630 635 640 645
cca ccg atg tcc gcc ggt att gtc cgc gga cct cgc ctg gtt ccc ggc 2083
Pro Pro Met Ser Ala Gly Ile Val Arg Gly Pro Arg Leu Val Pro Gly
650 655 660
gcc cca gtc ggc gac ggt cgt ttc cgt ctg ctc gcc gat cac ggc ggc 2131
Ala Pro Val Gly Asp Gly Arg Phe Arg Leu Leu Ala Asp His Gly Gly
665 670 675
gtc caa ggc gcg cgt ttc tgg cag gcc cgc gag atc gcc acc ggc aag 2179
Val Gln Gly Ala Arg Phe Trp Gln Ala Arg Glu Ile Ala Thr Gly Lys
680 685 690
gaa gtc gcg ctg atc ttt gtg gat act tcc ggc aac gcc cca ttt gcg 2227
Glu Val Ala Leu Ile Phe Val Asp Thr Ser Gly Asn Ala Pro Phe Ala
695 700 705
cca ctg tct tcg gca gcc gca gcg ggc atc gcc tac gag gtg cag cgc 2275
Pro Leu Ser Ser Ala Ala Ala Ala Gly Ile Ala Tyr Glu Val Gln Arg
710 715 720 725
cgc acc aag aag ctg gcc agc ttg ggc agc ttg gcg gta gcc ccc aac 2323
Arg Thr Lys Lys Leu Ala Ser Leu Gly Ser Leu Ala Val Ala Pro Asn
730 735 740
atc cac tcc gag gcg tac cgc aac ggt tgc ctc att gtg gcc gat tgg 2371
Ile His Ser Glu Ala Tyr Arg ASn Gly Cys Leu Ile Val Ala Asp Trp
745 750 755
gtg cct ggc tcc agc ttg agc gcc gtc gcg gaa tcc ggt gcc gat ccc 2419
Val Pro Gly Ser Ser Leu Ser Ala Val Ala Glu Ser Gly Ala Asp Pro
760 765 770
cgc gcc gcc gcg ttc gcg ctc gcg gaa cta act gaa acc atc ggc gag 2467
Arg Ala Ala Ala Phe Ala Leu Ala Glu Leu Thr Glu Thr Ile Gly Glu
775 780 785
gcc cac gag atg ggt atc ccg gcc ggc ttg gac aac aag tgc cgc atc 2515
Ala His Glu Met Gly Ile Pro Ala Gly Leu Asp Asn Lys Cys Arg Ile
790 795 800 805
cgc atc aac acc gac ggc cat gcc gtc ctc gcc ttc ccg gcg att ttg 2563
Arg Ile Asn Thr Asp Gly His Ala Val Leu Ala Phe Pro Ala Ile Leu
810 815 820
ccc gat gcc tca gag ctc cgc gac gcc aag tcc ctg gcc tcg gcc gcc 2611
Pro Asp Ala Ser Glu Leu Arg Asp Ala Lys Ser Leu Ala Ser Ala Ala
825 830 835
gag atg ctt atc gac gcg acc ctc gct ccc agc gac gtc aag gca atg 2659
Glu Met Leu Ile Asp Ala Thr Leu Ala Pro Ser Asp Val Lys Ala Met
840 845 850
gtc act gaa gcc cag ggg cta gct aca gaa gac aat ccc gat tac gca 2707
Val Thr Glu Ala Gln Gly Leu Ala Thr Glu Asp Asn Pro Asp Tyr Ala
855 860 865
tca ctt gcc atg gcg atg cgc acc tgc gga ctg ttc acc gag gaa cca 2755
Ser Leu Ala Met Ala Met Arg Thr Cys Gly Leu Phe Thr Glu Glu Pro
870 875 880 885
acc cac ctt gtg gtg aag aag gaa aag aca cca aag cct gcg aca cgt 2803
Thr His Leu Val Val Lys Lys Glu Lys Thr Pro Lys Pro Ala Thr Arg
890 895 900
gat ggt ttc ggt gcc tcc gac tac acc gtc aag ggc atg gca gcc atc 2851
Asp Gly Phe Gly Ala Ser Asp Tyr Thr Val Lys Gly Met Ala Ala Ile
905 910 915
gcc gct gtg gtg atc atc ttg gtt tcc ctg gtg gcc gcc ggt acc gcg 2899
Ala Ala Val Val Ile Ile Leu Val Ser Leu Val Ala Ala Gly Thr Ala
920 925 930
ttc ctc acc agc ttc ttc ggc agc agc acc aac gaa caa tcc ccg ttg 2947
Phe Leu Thr Ser Phe Phe Gly Ser Ser Thr Asn Glu Gln Ser Pro Leu
935 940 945
gcc tct gtt gaa gcc acc acc tct gca aca cca gaa cct gtg ggg cca 2995
Ala Ser Val Glu Ala Thr Thr Ser Ala Thr Pro Glu Pro Val Gly Pro
950 955 960 965
ccg gtc tac ctg gat ctg gat caa gcc cgc acg tgg gat gac ggt gca 3043
Pro Val Tyr Leu Asp Leu Asp Gln Ala Arg Thr Trp Asp Asp Gly Ala
970 975 980
gga aca gat gtc acc gac gtc acc gac ggc aac acc tcc acc gca tgg 3091
Gly Thr Asp Val Thr Asp Val Thr Asp Gly Asn Thr Ser Thr Ala Trp
985 990 995
acc tcc acc ggc ggc gac ggc ctc cta gtt gac ctg tcc acg cct gcc 3139
Thr Ser Thr Gly Gly Asp Gly Leu Leu Val Asp Leu Ser Thr Pro Ala
1000 1005 1010
cgc ctc gac cgc gtc atc ttg acc acc ggc acc ggc tcc gac agc aac 3187
Arg Leu Asp Arg Val Ile Leu Thr Thr Gly Thr Gly Ser Asp Ser Asn
1015 1020 1025
gtg acc tcg acc gtg aag atc tac gca ttc aac gac gcc tca cca cac 3235
Val Thr Ser Thr Val Lys Ile Tyr Ala Phe Asn Asp Ala Ser Pro His
1030 1035 1040 1045
tcc ctg tcg gaa ggc atc gag atc ggc acc gtg gat tat tcc ggc cgc 3283
Ser Leu Ser Glu Gly Ile Glu Ile Gly Thr Val Asp Tyr Ser Gly Arg
1050 1055 1060
agt ctc agc cac agc atc cgc gat tcc tcc aag ctt ccg ggt cag gtg 3331
Ser Leu Ser His Ser Ile Arg Asp Ser Ser Lys Leu Pro Gly Gln Val
1065 1070 1075
gaa tcc gtg gtg att ctg gtc gat gag gtt cgt tcc tca caa acc tca 3379
Glu Ser Val Val Ile Leu Val Asp Glu Val Arg Ser Ser Gln Thr Ser
1080 1085 1090
gac acc aat cca cag atg cag atc gct gaa gta caa ctt gtt ggt tgg 3427
Asp Thr Asn Pro Gln Met Gln Ile Ala Glu Val Gln Leu Val Gly Trp
1095 1100 1105
taaattacgc gtttgtgatt gacccccatt 3457
<210>148
<211>1109
<212>PRT
<213>谷氨酸棒状杆菌
<400>148
Val Ser Ser Ser Leu Ser Asn Asn Ser Glu Gln Ser Gly Leu Arg Gly
1 5 10 15
Arg Ile Val Ala Pro Ala Pro Pro Ala Pro Val Pro Glu Ala Arg Lys
20 25 30
Lys Ala Val Ala Arg Thr Asp Gly Asp Arg Ser Ser Leu Lys Asn Ser
35 40 45
Pro Thr Ala Ser Ala Thr Gln Ala Ala Gln Thr Arg Pro Ala Glu Pro
50 55 60
Glu Pro Lys Lys His Thr Ser Asp Ser Asp Val Val Arg Ser Thr Gly
65 70 75 80
Ser Met Ala Ile Ala Thr Leu Leu Ser Arg Ile Thr Gly Phe Leu Arg
85 90 95
Thr Val Met Ile Gly Ala Ala Leu Ser Pro Ala Ile Ala Ser Ala Phe
100 105 110
Asn Thr Ala Asn Thr Leu Pro Asn Leu Ile Thr Glu Ile Val Leu Gly
115 120 125
Ala Val Leu Thr Ser Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys
130 135 140
Glu Asp Ala Asp Gly Gly Ser Gly Phe Phe Arg Arg Leu Leu Thr Leu
145 150 155 160
Ser Val Thr Leu Leu Gly Gly Val Thr Ile Leu Ser Ile Ile Gly Ala
165 170 175
Pro Leu Leu Thr Arg Met Met Leu Ser Ser Glu Gly Gln Val Asn Val
180 185 190
Val Met Ser Thr Ala Phe Ala Tyr Trp Leu Leu Pro Gln Ile Phe Phe
195 200 205
Tyr Gly Leu Phe Ala Leu Phe Met Ala Val Leu Asn Thr Arg Glu Val
210 215 220
Phe Lys Pro Gly Ala Trp Ala Pro Val Val Asn Asn Val Ile Thr Leu
225 230 235 240
Thr Val Leu Gly Val Tyr Met Val Leu Pro Ala Arg Leu His Pro His
245 250 255
Glu Gln Val Gly Ile Phe Asp Pro Gln Ile Ile Phe Leu Gly Val Gly
260 265 270
Thr Thr Leu Gly Val Val Ala Gln Cys Leu Ile Met Ile Pro Tyr Leu
275 280 285
Arg Arg Ala Gly Ile Asp Met Arg Pro Leu Trp Gly Ile Asp Ala Arg
290 295 300
Leu Lys Gln Phe Gly Gly Met Ala Met Ala Ile Ile Val Tyr Val Ala
305 310 315 320
Ile Ser Gln Phe Gly Tyr Ile Ile Thr Thr Arg Ile Ala Ser Ile Ala
325 330 335
Asp Asp Ala Ala Pro Phe Ile Tyr Gln Gln His Trp Met Leu Leu Gln
340 345 350
Val Pro Tyr Gly Ile Ile Gly Val Thr Leu Leu Thr Ala Ile Met Pro
355 360 365
Arg Leu Ser Arg Asn Ala Ala Asp Gly Asp Asp Arg Ala Val Val Ser
370 375 380
Asp Leu Gln Leu Gly Ser Lys Leu Thr Phe Ile Ala Leu Ile Pro Ile
385 390 395 400
Val Val Phe Phe Thr Ala Phe Gly Val Pro Ile Ala Asn Gly Leu Phe
405 410 415
Ala Tyr Gly Gln Phe Asp Ala Asn Ala Ala Asn Ile Leu Gly Trp Thr
420 425 430
Leu Ser Phe Ser Ala Phe Thr Leu Ile Pro Tyr Ala Leu Val Leu Leu
435 440 445
His Leu Arg Val Phe Tyr Ala Arg Glu Glu Val Trp Thr Pro Thr Phe
450 455 460
Ile Ile Ala Gly Ile Thr Ala Thr Lys Val Val Leu Ser Leu Leu Ala
465 470 475 480
Pro Leu Leu Ser Ser Ser Pro Glu Arg Val Val Val Leu Leu Gly Ala
485 490 495
Ala Ash Gly Phe Ser Phe Ile Thr Gly Ala Val Ile Gly Ala Tyr Leu
500 505 510
Leu Arg Asn Lys Leu Gly Leu Leu Gly Met Arg Ser Leu Ala Lys Thr
515 520 525
Ser Leu Trp Ala Leu Gly Ser Ala Ala Val Gly Ala Ala Ala Ala Trp
530 535 540
Ala Leu Gly Trp Leu Ile Gln Ala Val Val Gly Asp Phe Leu Leu Gly
545 550 555 560
Thr Leu Ser Ser Val Gly Tyr Leu Leu Asn Leu Ala Val Leu Gly Val
565 570 575
Phe Phe Ile Phe Val Thr Gly Ile Val Leu Ser Arg Ser Gly Leu Pro
580 585 590
Glu Val Gln Asn Leu Gly Gln Ala Leu Thr Arg Ile Pro Gly Leu Ser
595 600 605
Arg Phe Ile Arg Pro Asn Thr Lys Ile Ser Leu Asp Val Gly Glu Val
610 615 620
Ser Glu Gln Asp Phe Ser Thr Gln Leu Val Ala Pro Ser Glu Phe Ala
625 630 635 640
Ala Thr Pro Val Pro Pro Pro Met Ser Ala Gly Ile Val Arg Gly Pro
645 650 655
Arg Leu Val Pro Gly Ala Pro Val Gly Asp Gly Arg Phe Arg Leu Leu
660 665 670
Ala Asp His Gly Gly Val Gln Gly Ala Arg Phe Trp Gln Ala Arg Glu
675 680 685
Ile Ala Thr Gly Lys Glu Val Ala Leu Ile Phe Val Asp Thr Ser Gly
690 695 700
Asn Ala Pro Phe Ala Pro Leu Ser Ser Ala Ala Ala Ala Gly Ile Ala
705 710 715 720
Tyr Glu Val Gln Arg Arg Thr Lys Lys Leu Ala Ser Leu Gly Ser Leu
725 730 735
Ala Val Ala Pro Asn Ile His Ser Glu Ala Tyr Arg Asn Gly Cys Leu
740 745 750
Ile Val Ala Asp Trp Val Pro Gly Ser Ser Leu Ser Ala Val Ala Glu
755 760 765
Ser Gly Ala Asp Pro Arg Ala Ala Ala Phe Ala Leu Ala Glu Leu Thr
770 775 780
Glu Thr Ile Gly Glu Ala His Glu Met Gly Ile Pro Ala Gly Leu Asp
785 790 795 800
Asn Lys Cys Arg Ile Arg Ile Asn Thr Asp Gly His Ala Val Leu Ala
805 810 815
Phe Pro Ala Ile Leu Pro Asp Ala Ser Glu Leu Arg Asp Ala Lys Ser
820 825 830
Leu Ala Ser Ala Ala Glu Met Leu Ile Asp Ala Thr Leu Ala Pro Ser
835 840 845
Asp Val Lys Ala Met Val Thr Glu Ala Gln Gly Leu Ala Thr Glu Asp
850 855 860
Asn Pro Asp Tyr Ala Ser Leu Ala Met Ala Met Arg Thr Cys Gly Leu
865 870 875 880
Phe Thr Glu Glu Pro Thr His Leu Val Val Lys Lys Glu Lys Thr Pro
885 890 895
Lys Pro Ala Thr Arg Asp Gly Phe Gly Ala Ser Asp Tyr Thr Val Lys
900 905 910
Gly Met Ala Ala Ile Ala Ala Val Val Ile Ile Leu Val Ser Leu Val
915 920 925
Ala Ala Gly Thr Ala Phe Leu Thr Ser Phe Phe Gly Ser Ser Thr Asn
930 935 940
Glu Gln Ser Pro Leu Ala Ser Val Glu Ala Thr Thr Ser Ala Thr Pro
945 950 955 960
Glu Pro Val Gly Pro Pro Val Tyr Leu Asp Leu Asp Gln Ala Arg Thr
965 970 975
Trp Asp Asp Gly Ala Gly Thr Asp Val Thr Asp Val Thr Asp Gly Asn
980 985 990
Thr Ser Thr Ala Trp Thr Ser Thr Gly Gly Asp Gly Leu Leu Val Asp
995 1000 1005
Leu Ser Thr Pro Ala Arg Leu Asp Arg Val Ile Leu Thr Thr Gly Thr
1010 1015 1020
Gly Ser Asp Ser Asn Val Thr Ser Thr Val Lys Ile Tyr Ala Phe Asn
1025 1030 1035 1040
Asp Ala Ser Pro His Ser Leu Ser Glu Gly Ile Glu Ile Gly Thr Val
1045 1050 1055
Asp Tyr Ser Gly Arg Ser Leu Ser His Ser Ile Arg Asp Ser Ser Lys
1060 1065 1070
Leu Pro Gly Gln Val Glu Ser Val Val Ile Leu Val Asp Glu Val Arg
1075 1080 1085
Ser Ser Gln Thr Ser Asp Thr Asn Pro Gln Met Gln Ile Ala Glu Val
1090 1095 1100
Gln Leu Val Gly Trp
1105
<210>149
<211>676
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(646)
<223>RXA01439
<400>149
gttaatccac agcgccctgt gtgcactcta ttagtgaata tccggtgaat gctcgtccct 60
cgactgtcta cttagtaggt ttgcaggcta gtcttttgtc atg acc cga tac ttt 115
Met Thr Arg Tyr Phe
1 5
gca gtt tcc aat ctt cag gag ctt ggc tcc ctc gaa gta cac aaa ttg 163
Ala Val Ser Asn Leu Gln Glu Leu Gly Ser Leu Glu Val His Lys Leu
10 15 20
tac aaa ctt cgt gta gat att ttc gtc cac gag cag cag act ccg tac 211
Tyr Lys Leu Arg Val Asp Ile Phe Val His Glu Gln Gln Thr Pro Tyr
25 30 35
gcg gag atc gat gac acc gat gct gcc cca acc acc aat cac atc ctg 259
Ala Glu Ile Asp Asp Thr Asp Ala Ala Pro Thr Thr Asn His Ile Leu
40 45 50
gtt tgg gag cgc gcc gac gcc acc cca acc aac ctc atc ggc tgc gca 307
Val Trp Glu Arg Ala Asp Ala Thr Pro Thr Asn Leu Ile Gly Cys Ala
55 60 65
cgc ctc gcg ccc atc acc gcc gcc gag ctt aag gcg tac acc ggc aag 355
Arg Leu Ala Pro Ile Thr Ala Ala Glu Leu Lys Ala Tyr Thr Gly Lys
70 75 80 85
ggc att tcg ctt gac gac gcc acc ccg ctc tca caa ctc ggc cgc gta 403
Gly Ile Ser Leu Asp Asp Ala Thr Pro Leu Ser Gln Leu Gly Arg Val
90 95 100
gcg gtg gca cag gaa ggg cgg ggc tcg ggg ctc tca ggt gag ctc atg 451
Ala Val Ala Gln Glu Gly Arg Gly Ser Gly Leu Ser Gly Glu Leu Met
105 110 115
cgc aac gcg ctg cgt ctt gca tat gag cag tac ccc gac cgc gat gtt 499
Arg Asn Ala Leu Arg Leu Ala Tyr Glu Gln Tyr Pro Asp Arg Asp Val
120 125 130
gtc ctg acc gcg cag aag cct ttg gta gat ttc tac gca gaa tac ggc 547
Val Leu Thr Ala Gln Lys Pro Leu Val Asp Phe Tyr Ala Glu Tyr Gly
135 140 145
ttt gag gtc ttg ggt gag gaa tac ctt gac tcg ggt gtt cct cac ctt 595
Phe Glu Val Leu Gly Glu Glu Tyr Leu Asp Ser Gly Val Pro His Leu
150 155 160 165
ccg atg ttg ctc aag gct gat gag ctt gaa agg ttc tca gac cta gac 643
Pro Met Leu Leu Lys Ala Asp Glu Leu Glu Arg Phe Ser Asp Leu Asp
170 175 180
gcc taatccgtag tcactggaac tcacccacat 676
Ala
<210>150
<211>182
<212>PRT
<213>谷氨酸棒状杆菌
<400>150
Met Thr Arg Tyr Phe Ala Val Ser Asn Leu Gln Glu Leu Gly Ser Leu
1 5 10 15
Glu Val His Lys Leu Tyr Lys Leu Arg Val Asp Ile Phe Val His Glu
20 25 30
Gln Gln Thr Pro Tyr Ala Glu Ile Asp Asp Thr Asp Ala Ala Pro Thr
35 40 45
Thr Asn His Ile Leu Val Trp Glu Arg Ala Asp Ala Thr Pro Thr Asn
50 55 60
Leu Ile Gly Cys Ala Arg Leu Ala Pro Ile Thr Ala Ala Glu Leu Lys
65 70 75 80
Ala Tyr Thr Gly Lys Gly Ile Ser Leu Asp Asp Ala Thr Pro Leu Ser
85 90 95
Gln Leu Gly Arg Val Ala Val Ala Gln Glu Gly Arg Gly Ser Gly Leu
100 105 110
Ser Gly Glu Leu Met Arg Asn Ala Leu Arg Leu Ala Tyr Glu Gln Tyr
115 120 125
Pro Asp Arg Asp Val Val Leu Thr Ala Gln Lys Pro Leu Val Asp Phe
130 135 140
Tyr Ala Glu Tyr Gly Phe Glu Val Leu Gly Glu Glu Tyr Leu Asp Ser
145 150 155 160
Gly Val Pro His Leu Pro Met Leu Leu Lys Ala Asp Glu Leu Glu Arg
165 170 175
Phe Ser Asp Leu Asp Ala
180
<210>151
<211>1159
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1129)
<223>RXA01441
<400>151
ttataacaat tagccgtgag ggtattaatc aaggtttcgg gagactaacg ttttagaaac 60
aagtggactc cagcacacct cgtgagatgg caggttcatc atg gct aga acg aca 115
Met Ala Arg Thr Thr
1 5
aca gcg cac gca gtt ctc atg gcg ctt agt tca gtc ggg ctt ttg atg 163
Thr Ala His Ala Val Leu Met Ala Leu Ser Ser Val Gly Leu Leu Met
10 15 20
ctc cag gcc tgc tcc gaa cag gaa caa acc cca gaa caa acg cca gcg 211
Leu Gln Ala Cys Ser Glu Gln Glu Gln Thr Pro Glu Gln Thr Pro Ala
25 30 35
ccc acc ctg act tcg cag gct ccc gag act cct caa gcc tca aca acc 259
Pro Thr Leu Thr Ser Gln Ala Pro Glu Thr Pro Gln Ala Ser Thr Thr
40 45 50
tcc tca gca agc tcc aca gca act ccc ata gca act ccc ata gca gcc 307
Ser Ser Ala Ser Ser Thr Ala Thr Pro Ile Ala Thr Pro Ile Ala Ala
55 60 65
acc gag ttg gat tat ctc cac atc gat cca gca acg tat gac atc ggt 355
Thr Glu Leu Asp Tyr Leu His Ile Asp Pro Ala Thr Tyr Asp Ile Gly
70 75 80 85
gtc caa gac caa gaa act gcc gta ttt acc act ggc gat ggg atc aca 403
Val Gln Asp Gln Glu Thr Ala Val Phe Thr Thr Gly Asp Gly Ile Thr
90 95 100
gcg cag tgt ttc ttc gag gca aca ccg ggg gag acc agc tat cag atc 451
Ala Gln Cys Phe Phe Glu Ala Thr Pro Gly Glu Thr Ser Tyr Gln Ile
105 110 115
aag gag ttt gat ttc gac gaa act gcg gga acg tgc gct ttt ggt gat 499
Lys Glu Phe Asp Phe Asp Glu Thr Ala Gly Thr Cys Ala Phe Gly Asp
120 125 130
caa cac atc agc gtc acc acg gat gaa aat gtc cgg gaa cgc ttt gcc 547
Gln His Ile Ser Val Thr Thr Asp Glu Asn Val Arg Glu Arg Phe Ala
135 140 145
gaa ttg agt gaa acc gaa gaa gaa tta cca gaa gcg caa gcg act ctc 595
Glu Leu Ser Glu Thr Glu Glu Glu Leu Pro Glu Ala Gln Ala Thr Leu
150 155 160 165
gat gtt ggt gag atg gtg cat ctt ggt cac atg ggt tgt tgg gcg ccg 643
Asp Val Gly Glu Met Val His Leu Gly His Met Gly Cys Trp Ala Pro
170 175 180
agt gta tcg gag ttt agt tgt ctc gat ttc gcc tcc aac cag gcg ttc 691
Ser Val Ser Glu Phe Ser Cys Leu Asp Phe Ala Ser Asn Gln Ala Phe
185 190 195
acc atg aat gaa cag ggc ttc cat gag ctt gat cct gca aaa gcc act 739
Thr Met Asn Glu Gln Gly Phe His Glu Leu Asp Pro Ala Lys Ala Thr
200 205 210
gag cag cta att aat tcg agt ggc cag gtt caa acg tta tcg aaa atg 787
Glu Gln Leu Ile Asn Ser Ser Gly Gln Val Gln Thr Leu Ser Lys Met
215 220 225
acg aat ttc cag ttc acc gat ggc acc agc atc acc tgt gtc agt gag 835
Thr Asn Phe Gln Phe Thr Asp Gly Thr Ser Ile Thr Cys Val Ser Glu
230 235 240 245
ttg cag gcg gaa gag ttc ttg tgc cac aac agt ggt cct gga ggc tgg 883
Leu Gln Ala Glu Glu Phe Leu Cys His Asn Ser Gly Pro Gly Gly Trp
250 255 260
tca act tca gct ggc cct gcc aac acc ctg tgg tgg aat ctg aac cag 931
Ser Thr Ser Ala Gly Pro Ala Asn Thr Leu Trp Trp Asn Leu Asn Gln
265 270 275
gcc gac tcc gag ttt gag ggt gct cgc ccc acg aat cca acg caa agc 979
Ala Asp Ser Glu Phe Glu Gly Ala Arg Pro Thr Asn Pro Thr Gln Ser
280 285 290
gtt tat aaa tct caa caa ata ttc ggt ccg gga tcg tat ctc ctc gcg 1027
Val Tyr Lys Ser Gln Gln Ile Phe Gly Pro Gly Ser Tyr Leu Leu Ala
295 300 305
aac ggt gtg agt gca gaa ttt gat gga acc acc ctc acg ctg acc acg 1075
Asn Gly Val Ser Ala Glu Phe Asp Gly Thr Thr Leu Thr Leu Thr Thr
310 315 320 325
cct cag ggg aat cag tat tgg gca aac aca cac gat ttt gga gcc ggc 1123
Pro Gln Gly Asn Gln Tyr Trp Ala Asn Thr His Asp Phe Gly Ala Gly
330 335 340
aca cac taattttagg agcactgatg aaaaccaagt 1159
Thr His
<210>152
<211>343
<212>PRT
<213>谷氨酸棒状杆菌
<400>152
Met Ala Arg Thr Thr Thr Ala His Ala Val Leu Met Ala Leu Ser Ser
1 5 10 15
Val Gly Leu Leu Met Leu Gln Ala Cys Ser Glu Gln Glu Gln Thr Pro
20 25 30
Glu Gln Thr Pro Ala Pro Thr Leu Thr Ser Gln Ala Pro Glu Thr Pro
35 40 45
Gln Ala Ser Thr Thr Ser Ser Ala Ser Ser Thr Ala Thr Pro Ile Ala
50 55 60
Thr Pro Ile Ala Ala Thr Glu Leu Asp Tyr Leu His Ile Asp Pro Ala
65 70 75 80
Thr Tyr Asp Ile Gly Val Gln Asp Gln Glu Thr Ala Val Phe Thr Thr
85 90 95
Gly Asp Gly Ile Thr Ala Gln Cys Phe Phe Glu Ala Thr Pro Gly Glu
100 105 110
Thr Ser Tyr Gln Ile Lys Glu Phe Asp Phe Asp Glu Thr Ala Gly Thr
115 120 125
Cys Ala Phe Gly Asp Gln His Ile Ser Val Thr Thr Asp Glu Asn Val
130 135 140
Arg Glu Arg Phe Ala Glu Leu Ser Glu Thr Glu Glu Glu Leu Pro Glu
145 150 155 160
Ala Gln Ala Thr Leu Asp Val Gly Glu Met Val His Leu Gly His Met
165 170 175
Gly Cys Trp Ala Pro Ser Val Ser Glu Phe Ser Cys Leu Asp Phe Ala
180 185 190
Ser Asn Gln Ala Phe Thr Met Asn Glu Gln Gly Phe His Glu Leu Asp
195 200 205
Pro Ala Lys Ala Thr Glu Gln Leu Ile Asn Ser Ser Gly Gln Val Gln
210 215 220
Thr Leu Ser Lys Met Thr Asn Phe Gln Phe Thr Asp Gly Thr Ser Ile
225 230 235 240
Thr Cys Val Ser Glu Leu Gln Ala Glu Glu Phe Leu Cys His Asn Ser
245 250 255
Gly Pro Gly Gly Trp Ser Thr Ser Ala Gly Pro Ala Asn Thr Leu Trp
260 265 270
Trp Asn Leu Asn Gln Ala Asp Ser Glu Phe Glu Gly Ala Arg Pro Thr
275 280 285
Asn Pro Thr Gln Ser Val Tyr Lys Ser Gln Gln Ile Phe Gly Pro Gly
290 295 300
Ser Tyr Leu Leu Ala Asn Gly Val Ser Ala Glu Phe Asp Gly Thr Thr
305 310 315 320
Leu Thr Leu Thr Thr Pro Gln Gly Asn Gln Tyr Trp Ala Asn Thr His
325 330 335
Asp Phe Gly Ala Gly Thr His
340
<210>153
<211>1297
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1267)
<223>RXA01448
<400>153
gtaatctcag cagttaaaaa gggcagttcc gcatcagggg gactgccctt tttcgtgtct 60
tctcatgctc ttgtgaatat gcatagctaa tcttgaggcc atg gca aag aag aaa 115
Met Ala Lys Lys Lys
1 5
ctg ggg act gtc gcc aga ctg tct gaa ctg gat aag tcc ctg cgc aat 163
Leu Gly Thr Val Ala Arg Leu Ser Glu Leu Asp Lys Ser Leu Arg Asn
10 15 20
aga ttg ctg cgg gtt cgt tcc cga ctg ctg ttt att gtt cat tcc gca 211
Arg Leu Leu Arg Val Arg Ser Arg Leu Leu Phe Ile Val His Ser Ala
25 30 35
att ggt gcg ggt gtg gcg tat tgg atc gcc gtg gaa gtg atc aaa cac 259
Ile Gly Ala Gly Val Ala Tyr Trp Ile Ala Val Glu Val Ile Lys His
40 45 50
gga caa ccg ttt ttc gca ccg atg tcc gcg gtg att att ttg ggt ctc 307
Gly Gln Pro Phe Phe Ala Pro Met Ser Ala Val Ile Ile Leu Gly Leu
55 60 65
tcc gga gga gac cgc atc aaa aga gcc acg gaa ctc acc ctg ggc tgt 355
Ser Gly Gly Asp Arg Ile Lys Arg Ala Thr Glu Leu Thr Leu Gly Cys
70 75 80 85
gct tta ggc gtt ggt ttg ggt gat tta ctg atc atg caa atc ggc acg 403
Ala Leu Gly Val Gly Leu Gly Asp Leu Leu Ile Met Gln Ile Gly Thr
90 95 100
ggc tat tgg cag ata ttt gtg gta gtt gga tta gcg ctg ctg gtg gcc 451
Gly Tyr Trp Gln Ile Phe Val Val Val Gly Leu Ala Leu Leu Val Ala
105 110 115
tcg ttt gtt tca ccg gca ccg ttg gtg agt aat cag atg gcc att ggt 499
Ser Phe Val Ser Pro Ala Pro Leu Val Ser Asn Gln MetAla Ile Gly
120 125 130
ggc att ttg att gcc acg atg ttc ccg cca ggt gat ggt gga agc att 547
Gly Ile Leu Ile Ala Thr Met Phe Pro Pro Gly Asp Gly Gly Ser Ile
135 140 145
gac cgt atg atc gac gcc ttc att ggt ggt ggc gtg gga att ttg gtc 595
Asp Arg Met Ile Asp Ala Phe Ile Gly Gly Gly Val Gly Ile Leu Val
150 155 160 165
atc gca ctt ctt ccc tca tct cct ctt gac gca ggc cgg cat caa gtg 643
Ile Ala Leu Leu Pro Ser Ser Pro Leu Asp Ala Gly Arg His Gln Val
170 175 180
gcc aac gtt ttg ggg atc gca gct agt gtg ctg gaa gac gtg gca gct 691
Ala Asn Val Leu Gly Ile Ala Ala Ser Val Leu Glu Asp Val Ala Ala
185 190 195
tcc cta aaa gcc aag gat gca gcc aaa ctc aac aat gct ttg gaa gca 739
Ser Leu Lys Ala Lys Asp Ala Ala Lys Leu Asn Asn Ala Leu Glu Ala
200 205 210
ttg aga agg tcg cag gcg tcg gtg aac aag ctg gaa act gcg gca tct 787
Leu Arg Arg Ser Gln Ala Ser Val Asn Lys Leu Glu Thr Ala Ala Ser
215 220 225
tca ggc aag gaa gca acc acc gta tcg cca ttt tta tgg gga gat agg 835
Ser Gly Lys Glu Ala Thr Thr Val Ser Pro Phe Leu Trp Gly Asp Arg
230 235 240 245
gcc cgc gtg aga tcg ctg tat cgc att ctg gcg cca gtg gac aac gtc 883
Ala Arg Val Arg Ser Leu Tyr Arg Ile Leu Ala Pro Val Asp Asn Val
250 255 260
atc cga aat gct cga gtc ctt gcg cga cgg gca gtg gtg ctg acc gaa 931
Ile Arg Asn Ala Arg Val Leu Ala Arg Arg Ala Val Val Leu Thr Glu
265 270 275
gac aat gac acc gtc agt gat gaa caa atc cac gtg att gag gaa atc 979
Asp Asn Asp Thr Val Ser Asp Glu Gln Ile His Val Ile Glu GluIle
280 285 290
gca gac att gca ctg cga ctg tca gac ctt tat gag cac cac aaa gaa 1027
Ala Asp Ile Ala Leu Arg Leu Ser Asp Leu Tyr Glu His His Lys Glu
295 300 305
atc agt gaa gca ctg gaa att cct gag ttg gtt aac cga ctg cgt caa 1075
Ile Ser Glu Ala Leu Glu Ile pro Glu Leu Val Asn Arg Leu Arg Gln
310 315 320 325
ctg ggc agt gaa gtg ggc gag gac atc gcc gaa gat cga gtg cta tcc 1123
Leu Gly Ser Glu Val Gly Glu Asp Ile Ala Glu Asp Arg Val Leu Ser
330 335 340
gca caa gta att ttg gcg caa tcg cga tcc atc att gtg gac ctg ttg 1171
Ala Gln Val Ile Leu Ala Gln Ser Arg Ser Ile Ile Val Asp Leu Leu
345 350 355
cag atc tgc ggc atg tcc agg gaa tct gcg gtg gca gtg ttg gtt ccg 1219
Gln Ile Cys Gly Met Ser Arg Glu Ser Ala Val Ala Val Leu Val Pro
360 365 370
acc tca gag agt ccg gct tac cct ccg gag ctg tgg gat gac gaa gac 1267
Thr Ser Glu Ser Pro Ala Tyr Pro Pro Glu Leu Trp Asp Asp Glu Asp
375 380 385
tagcctttta tcgcggttcg gttttcacgg 1297
<210>154
<211>389
<212>PRT
<213>谷氨酸棒状杆菌
<400>154
Met Ala Lys Lys Lys Leu Gly Thr Val Ala Arg Leu Ser Glu Leu Asp
1 5 10 15
Lys Ser Leu Arg Asn Arg Leu Leu Arg Val Arg Ser Arg Leu Leu Phe
20 25 30
Ile Val His Ser Ala Ile Gly Ala Gly Val Ala Tyr Trp Ile Ala Val
35 40 45
Glu Val Ile Lys His Gly Gln Pro Phe Phe Ala Pro Met Ser Ala Val
50 55 60
Ile Ile Leu Gly Leu Ser Gly Gly Asp Arg Ile Lys Arg Ala Thr Glu
65 70 75 80
Leu Thr Leu Gly Cys Ala Leu Gly Val Gly Leu Gly Asp Leu Leu Ile
85 90 95
Met Gln Ile Gly Thr Gly Tyr Trp Gln Ile Phe Val Val Val Gly Leu
100 105 110
Ala Leu Leu Val Ala Ser Phe Val Ser Pro Ala Pro Leu Val Ser Asn
115 120 125
Gln Met Ala Ile Gly Gly Ile Leu Ile Ala Thr Met Phe Pro Pro Gly
130 135 140
Asp Gly Gly Ser Ile Asp Arg Met Ile Asp Ala Phe Ile Gly Gly Gly
145 150 155 160
Val Gly Ile Leu Val Ile Ala Leu Leu Pro Ser Ser Pro Leu Asp Ala
165 170 175
Gly Arg His Gln Val Ala Asn Val Leu Gly Ile Ala Ala Ser Val Leu
180 185 190
Glu Asp Val Ala Ala Ser Leu Lys Ala Lys Asp Ala Ala Lys Leu Asn
195 200 205
Asn Ala Leu Glu Ala Leu Arg Arg Ser Gln Ala Ser Val Asn Lys Leu
210 215 220
Glu Thr Ala Ala Ser Ser Gly Lys Glu Ala Thr Thr Val Ser Pro Phe
225 230 235 240
Leu Trp Gly Asp Arg Ala Arg Val Arg Ser Leu Tyr Arg Ile Leu Ala
245 250 255
Pro Val Asp Asn Val Ile Arg Asn Ala Arg Val Leu Ala Arg Arg Ala
260 265 270
Val Val Leu Thr Glu Asp Asn Asp Thr Val Ser Asp Glu Gln Ile His
275 280 285
Val Ile Glu Glu Ile Ala Asp Ile Ala Leu Arg Leu Ser Asp Leu Tyr
290 295 300
Glu His His Lys Glu Ile Ser Glu Ala Leu Glu Ile Pro Glu Leu Val
305 310 315 320
Asn Arg Leu Arg Gln Leu Gly Ser Glu Val Gly Glu Asp Ile Ala Glu
325 330 335
Asp Arg Val Leu Ser Ala Gln Val Ile Leu Ala Gln Ser Arg Ser Ile
340 345 350
Ile Val Asp Leu Leu Gln Ile Cys Gly Met Ser Arg Glu Ser Ala Val
355 360 365
Ala Val Leu Val Pro Thr Ser Glu Ser Pro Ala Tyr Pro Pro Glu Leu
370 375 380
Trp Asp Asp Glu Asp
385
<210>155
<211>604
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(574)
<223>RXA01518
<400>155
agcagaacct gatgccgtcc tgcacggcac gaccattgca gaacatgtgg ataatcttga 60
tcccacagac attgaaggtg tcaccaagat ttaaggagtc gtg gct ttc atg cag 115
Val Ala Phe Met Gln
1 5
aaa acg tca gcg ggt tgg ttg atc gca acg gga ggt ttc ctc gcc gct 163
Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly Gly Phe Leu Ala Ala
10 15 20
gtg tcc gcc att ttg acg tgg cgt ttt tat gga tcc atg acc tct att 211
Val Ser Ala Ile Leu Thr Trp Arg Phe Tyr Gly Ser Met Thr Ser Ile
25 30 35
tcc atc acg gta tcc atc act ttt tgg ttg ttg gcc gtg gtg tgt ggt 259
Ser Ile Thr Val Ser Ile Thr Phe Trp Leu Leu Ala Val Val Cys Gly
40 45 50
ttc gca ggt gtg aag gtc caa ggt cgc ctc gat gag ggg ctg atc ggc 307
Phe Ala Gly Val Lys Val Gln Gly Arg Leu Asp Glu Gly Leu Ile Gly
55 60 65
cag gac aaa tcc caa atg aac ccc gtg acc att gcc tat ctg gcc atg 355
Gln Asp Lys Ser Gln Met Asn Pro Val Thr Ile Ala Tyr Leu Ala Met
70 75 80 85
ctg ggt cga gcg tgt gcg tgg ggt ggc gca att ttc ggc ggc gtt tat 403
Leu Gly Arg Ala Cys Ala Trp Gly Gly Ala Ile Phe Gly Gly Val Tyr
90 95 100
gtg gga att ggc agt tat gta atc cca cgc gcc ggt gag ttg tcc gca 451
Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala Gly Glu Leu Ser Ala
105 110 115
gca tcg aat gat ctt ccg gga gtt att gcc tgt gcg ctg ggc gga atc 499
Ala Ser Asn Asp Leu Pro Gly Val Ile Ala Cys Ala Leu Gly Gly Ile
120 125 130
gca ctc tca gct gcc gga ctt tat tta gag cga agc tgt gag gct ccg 547
Ala Leu Ser Ala Ala Gly Leu Tyr Leu Glu Arg Ser Cys Glu Ala Pro
135 140 145
cct ccc caa tct ggc gaa gcg atc agc tagattggaa ttcatgaatc 594
Pro Pro Gln Ser Gly Glu Ala Ile Ser
150 155
aagacctgtc 604
<210>156
<211>158
<212>PRT
<213>谷氨酸棒状杆菌
<400>156
Val Ala Phe Met Gln Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly
1 5 10 15
Gly Phe Leu Ala Ala Val Ser Ala Ile Leu Thr Trp Arg Phe Tyr Gly
20 25 30
Ser Met Thr Ser Ile Ser Ile Thr Val Ser Ile Thr Phe Trp Leu Leu
35 40 45
Ala Val Val Cys Gly Phe Ala Gly Val Lys Val Gln Gly Arg Leu Asp
50 55 60
Glu Gly Leu Ile Gly Gln Asp Lys Ser Gln Met Asn Pro Val Thr Ile
65 70 75 80
Ala Tyr Leu Ala Met Leu Gly Arg Ala Cys Ala Trp Gly Gly Ala Ile
85 90 95
Phe Gly Gly Val Tyr Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala
100 105 110
Gly Glu Leu Ser Ala Ala Ser Asn Asp Leu Pro Gly Val Ile Ala Cys
115 120 125
Ala Leu Gly Gly Ile Ala Leu Ser Ala Ala Gly Leu Tyr Leu Glu Arg
130 135 140
Ser Cys Glu Ala Pro Pro Pro Gln Ser Gly Glu Ala Ile Ser
145 150 155
<210>157
<211>1372
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1342)
<223>RXA01536
<400>157
agcatagatg acttgacact gttgcagcag gcggttttca cctggcctct taggatttgg 60
tcaaatggtt gcaacgacgc cagggaagga ggcgcacccc atg gca ttt gga ttt 115
Met Ala Phe Gly Phe
1 5
ttt agt aga cgt aag aaa aag aac aaa gac aaa aac ccg aat gaa aat 163
Phe Ser Arg Arg Lys Lys Lys Asn Lys Asp Lys Asn Pro Asn Glu Asn
10 15 20
tca gca gtg ccc gca cac tct gaa gat tca cct cag gag gtt ttt gag 211
Ser Ala Val Pro Ala His Ser Glu Asp Ser Pro Gln Glu Val Phe Glu
25 30 35
ggt aat ggt cgt cag gta ggc gac ccc att gaa cag cag gtt gat cga 259
Gly Asn Gly Arg Gln Val Gly Asp Pro Ile Glu Gln Gln Val Asp Arg
40 45 50
gat gct aaa ggt cgt ctc aca gcg gcg gat ttc ttg ccg gac gct gat 307
Asp Ala Lys Gly Arg Leu Thr Ala Ala Asp Phe Leu Pro Asp Ala Asp
55 60 65
ctg cca cag ctg aat cgt tcg cgt gca aat atg ctg cgc cgt gaa ttg 355
Leu Pro Gln Leu Asn Arg Ser Arg Ala Asn Met Leu Arg Arg Glu Leu
70 75 80 85
gag tac cgt ttt tca ctc cag aat gcc cac att aat atc gat gga aac 403
Glu Tyr Arg Phe Ser Leu Gln Asn Ala His Ile Asn Ile Asp Gly Asn
90 95 100
acg gcc atg att cag cgt tca gat ggc ggg gca gca cat gtc tcg ttg 451
Thr Ala Met Ile Gln Arg Ser Asp Gly Gly Ala Ala His Val Ser Leu
105 110 115
cgc acc ctc gcg atg aat gca gct ggc ctt gat aac ttt gat caa ctc 499
Arg Thr Leu Ala Met Asn Ala Ala Gly Leu Asp Asn Phe Asp Gln Leu
120 125 130
cct gaa ctg gtg gaa agc ttc gtt cac ggc acg ctg gcc gat gca aca 547
Pro Glu Leu Val Glu Ser Phe Val His Gly Thr Leu Ala Asp Ala Thr
135 140 145
tta aac gat ctt tct act gct gac ctg tat aaa gca ctg cgc ctt cgc 595
Leu Asn Asp Leu Ser Thr Ala Asp Leu Tyr Lys Ala Leu Arg Leu Arg
150 155 160 165
ctg ctg cca aca cct ggc gaa ggc gac gat cta gtt gag cat gga ctc 643
Leu Leu Pro Thr Pro Gly Glu Gly Asp Asp Leu Val Glu His Gly Leu
170 175 180
gac cgg gaa agc cag atc cgc gac gat tca atc ctg cgc acc ttc acc 691
Asp Arg Glu Ser Gln Ile Arg Asp Asp Ser Ile Leu Arg Thr Phe Thr
185 190 195
tct gac atg tcg atc gcg ctg gtg ctc gat acc gag cat gcc atc cgc 739
Ser Asp Met Ser Ile Ala Leu Val Leu Asp Thr Glu His Ala Ile Arg
200 205 210
atc cag cca ctc aaa gag ctc gag gag ttc gat gac ctc agc gcc cta 787
Ile Gln Pro Leu Lys Glu Leu Glu Glu Phe Asp Asp Leu Ser Ala Leu
215 220 225
gag cgg gct gcg gac cgc aat acc tgg caa gag ctt tac gac gca aac 835
Glu Arg Ala Ala Asp Arg Asn Thr Trp Gln Glu Leu Tyr Asp Ala Asn
230 235 240 245
gtt gac gct tcc ttc gtc gac gct gaa tca gac agc gaa ggg tca tca 883
Val Asp Ala Ser Phe Val Asp Ala Glu Ser Asp Ser Glu Gly Ser Ser
250 255 260
ttt tgg gct ttc gaa tct aac tcg tac tac ttg ggt agt gca cca ctg 931
Phe Trp Ala Phe Glu Ser Asn Ser Tyr Tyr Leu Gly Ser Ala Pro Leu
265 270 275
ttc ctc aac gat ctg ttg gca aag tgg gca cct gac ctg gac caa agt 979
Phe Leu Asn Asp Leu Leu Ala Lys Trp Ala Pro Asp Leu Asp Gln Ser
280 285 290
gat ggc gtc atc ttt gct gtc cct gat cgt gat ctg ttg att gcg cgt 1027
Asp Gly Val Ile Phe Ala Val Pro Asp Arg Asp Leu Leu Ile Ala Arg
295 300 305
cct gtg acc acc ggc gaa gat ctg atg aac gga atc acc gcg atg gtg 1075
Pro Val Thr Thr Gly Glu Asp Leu Met Asn Gly Ile Thr Ala Met Val
310 315 320 325
agg atc gcg atg cgc ttt ggc ctc ggg aac ccg acg tcg ata agc ccg 1123
Arg Ile Ala Met Arg Phe Gly Leu Gly Asn Pro Thr Ser Ile Ser Pro
330 335 340
cgc ctg cac ctg ctg cgc gac aac cag gtg acc acc ttc acc gac ttc 1171
Arg Leu His Leu Leu Arg Asp Asn Gln Val Thr Thr Phe Thr Asp Phe
345 350 355
cgc gtc gtc tct cct gaa atg gaa gct gaa tgg gaa gac agc gcg ttt 1219
Arg Val Val Ser Pro Glu Met Glu Ala Glu Trp Glu Asp Ser Ala Phe
360 365 370
gac gcg cca ccg gcc ggc gcg atc ggc att gag gtg cgc cca gat ccg 1267
Asp Ala Pro Pro Ala Gly Ala Ile Gly Ile Glu Val Arg Pro Asp Pro
375 380 385
tat ctg atg gag cgc ctc caa cag ggc ggc ttt ggt gat ttc gga gat 1315
Tyr Leu Met Glu Arg Leu Gln Gln Gly Gly Phe Gly Asp Phe Gly Asp
390 395 400 405
ttc ggc aag ccc cgc gat cta gat atg tagcgaaaaa gggaccttca 1362
Phe Gly Lys Pro Arg Asp Leu Asp Met
410
cttcttaaga 1372
<210>158
<211>414
<212>PRT
<213>谷氨酸棒状杆菌
<400>158
Met Ala Phe Gly Phe Phe Ser Arg Arg Lys Lys Lys Asn Lys Asp Lys
1 5 10 15
Asn Pro Asn Glu Asn Ser Ala Val Pro Ala His Ser Glu Asp Ser Pro
20 25 30
Gln Glu Val Phe Glu Gly Asn Gly Arg Gln Val Gly Asp Pro Ile Glu
35 40 45
Gln Gln Val Asp Arg Asp Ala Lys Gly Arg Leu Thr Ala Ala Asp Phe
50 55 60
Leu Pro Asp Ala Asp Leu Pro Gln Leu Asn Arg Ser Arg Ala Asn Met
65 70 75 80
Leu Arg Arg Glu Leu Glu Tyr Arg Phe Ser Leu Gln Asn Ala His Ile
85 90 95
Asn Ile Asp Gly Asn Thr Ala Met Ile Gln Arg Ser Asp Gly Gly Ala
100 105 110
Ala His Val Ser Leu Arg Thr Leu Ala Met Asn Ala Ala Gly Leu Asp
115 120 125
Asn Phe Asp Gln Leu Pro Glu Leu Val Glu Ser Phe Val His Gly Thr
130 135 140
Leu Ala Asp Ala Thr Leu Asn Asp Leu Ser Thr Ala Asp Leu Tyr Lys
145 150 155 160
Ala Leu Arg Leu Arg Leu Leu Pro Thr Pro Gly Glu Gly Asp Asp Leu
165 170 175
Val Glu His Gly Leu Asp Arg Glu Ser Gln Ile Arg Asp Asp Ser Ile
180 185 190
Leu Arg Thr Phe Thr Ser Asp Met Ser Ile Ala Leu Val Leu Asp Thr
195 200 205
Glu His Ala Ile Arg Ile Gln Pro Leu Lys Glu Leu Glu Glu Phe Asp
210 215 220
Asp Leu Ser Ala Leu Glu Arg Ala Ala Asp Arg Asn Thr Trp Gln Glu
225 230 235 240
Leu Tyr Asp Ala Asn Val Asp Ala Ser Phe Val Asp Ala Glu Ser Asp
245 250 255
Ser Glu Gly Ser Ser Phe Trp Ala Phe Glu Ser Asn Ser Tyr Tyr Leu
260 265 270
Gly Ser Ala Pro Leu Phe Leu Asn Asp Leu Leu Ala Lys Trp Ala Pro
275 280 285
Asp Leu Asp Gln Ser Asp Gly Val Ile Phe Ala Val Pro Asp Arg Asp
290 295 300
Leu Leu Ile Ala Arg Pro Val Thr Thr Gly Glu Asp Leu Met Asn Gly
305 310 315 320
Ile Thr Ala Met Val Arg Ile Ala Met Arg Phe Gly Leu Gly Asn Pro
325 330 335
Thr Ser Ile Ser Pro Arg Leu His Leu Leu Arg Asp Asn Gln Val Thr
340 345 350
Thr Phe Thr Asp Phe Arg Val Val Ser Pro Glu Met Glu Ala Glu Trp
355 360 365
Glu Asp Ser Ala Phe Asp Ala Pro Pro Ala Gly Ala Ile Gly Ile Glu
370 375 380
Val Arg Pro Asp Pro Tyr Leu Met Glu Arg Leu Gln Gln Gly Gly Phe
385 390 395 400
Gly Asp Phe Gly Asp Phe Gly Lys Pro Arg Asp Leu Asp Met
405 410
<210>159
<211>730
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(700)
<223>RXA01544
<400>159
ctagatctat aacgaggatc ggcgctactt taagcatgtt tgtgctgttg gcgccgattt 60
tttcatagct gtacacacat aaggcaacag gagaaataca gtg aaa ctt gac gac 115
Val Lys Leu Asp Asp
1 5
gct atg gtc atg atc gaa cag cgc ctg gtc aaa ggt cgc ggc gca acc 163
Ala Met Val Met Ile Glu Gln Arg Leu Val Lys Gly Arg Gly Ala Thr
10 15 20
aag acg caa cta gca ccc tat gag cat atc cag cgc aat att gca gcc 211
Lys Thr Gln Leu Ala Pro Tyr Glu His Ile Gln Arg Asn Ile Ala Ala
25 30 35
cga ggg ttg aga gcc gat gac gga ggc tcg tgc ttc ggt tat ggg gat 259
Arg Gly Leu Arg Ala Asp Asp Gly Gly Ser Cys Phe Gly Tyr Gly Asp
40 45 50
gcg tcg ctg agt aat gac gat gta gca act gct gca gaa gat att cag 307
Ala Ser Leu Ser Asn Asp Asp Val Ala Thr Ala Ala Glu Asp Ile Gln
55 60 65
gat ttg ttt gcg tgc ggg cat acg gtt atc tca gcg att gtc gtt ttt 355
Asp Leu Phe Ala Cys Gly His Thr Val Ile Ser Ala Ile Val Val Phe
70 75 80 85
gat aag agc tat ctc agt gag cat gga atc atg agc ttt gat ccg aac 403
Asp Lys Ser Tyr Leu Ser Glu His Gly Ile Met Ser Phe Asp Pro Asn
90 95 100
tat ttt ggt cag gat att tat gcg gac acc cat cca gag att gat ctc 451
Tyr Phe Gly Gln Asp Ile Tyr Ala Asp Thr His Pro Glu Ile Asp Leu
105 110 115
atg gaa ttg cgt tta gcc gtg atg aat ggg ctc ttg ggg ctt gag gga 499
Met Glu Leu Arg Leu Ala Val Met Asn Gly Leu Leu Gly Leu Glu Gly
120 125 130
acg ttt ttt gag gat atg cgt tat gtc gca tct atc gag gtg agc cgg 547
Thr Phe Phe Glu Asp Met Arg Tyr Val Ala Ser Ile Glu Val Ser Arg
135 140 145
agc tat gtc tat gcg cac ata atg atg gcg gat gca ggc gtt gac gat 595
Ser Tyr Val Tyr Ala His Ile Met Met Ala Asp Ala Gly Val Asp Asp
150 155 160 165
gca cgc ggt aat gca cag gtg aag att acg gac acc gaa cga gtg ctg 643
Ala Arg Gly Asn Ala Gln Val Lys Ile Thr Asp Thr Glu Arg Val Leu
170 175 180
ttt aga cgt ggt gtt gaa tcc cgg ttt gtg gag caa gag gca cgc aac 691
Phe Arg Arg Gly Val Glu Ser Arg Phe Val Glu Gln Glu Ala Arg Asn
185 190 195
gtc gtg ctt tagccaaggt gaggcatcaa gagaagcaac 730
Val Val Leu
200
<210>160
<211>200
<212>PRT
<213>谷氨酸棒状杆菌
<400>160
Val Lys Leu Asp Asp Ala Met Val Met Ile Glu Gln Arg Leu Val Lys
1 5 10 15
Gly Arg Gly Ala Thr Lys Thr Gln Leu Ala Pro Tyr Glu His Ile Gln
20 25 30
Arg Asn Ile Ala Ala Arg Gly Leu Arg Ala Asp Asp Gly Gly Ser Cys
35 40 45
Phe Gly Tyr Gly Asp Ala Ser Leu Ser Asn Asp Asp Val Ala Thr Ala
50 55 60
Ala Glu Asp Ile Gln Asp Leu Phe Ala Cys Gly His Thr Val Ile Ser
65 70 75 80
Ala Ile Val Val Phe Asp Lys Ser Tyr Leu Ser Glu His Gly Ile Met
85 90 95
Ser Phe Asp Pro Asn Tyr Phe Gly Gln Asp Ile Tyr Ala Asp Thr His
100 105 110
Pro Glu Ile Asp Leu Met Glu Leu Arg Leu Ala Val Met Asn Gly Leu
115 120 125
Leu Gly Leu Glu Gly Thr Phe Phe Glu Asp Met Arg Tyr Val Ala Ser
130 135 140
Ile Glu Val Ser Arg Ser Tyr Val Tyr Ala His Ile Met Met Ala Asp
145 150 155 160
Ala Gly Val Asp Asp Ala Arg Gly Asn Ala Gln Val Lys Ile Thr Asp
165 170 175
Thr Glu Arg Val Leu Phe Arg Arg Gly Val Glu Ser Arg Phe Val Glu
180 185 190
Gln Glu Ala Arg Asn Val Val Leu
195 200
<210>161
<211>1138
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1108)
<223>RXA01548
<400>161
ggcgtgcgag ctgctggttg gcgcttgtgc accccgtttt tgtgtaataa cttatattct 60
agaaacttca ataacttagt aactttaaaa ggggtttaat atg gct gag aat aat 115
Met Ala Glu Asn Asn
1 5
ggc ggg tcg gat gac ctt aag ccc atc tct gtc gat ggg gtc gtc aat 163
Gly Gly Ser Asp Asp Leu Lys Pro Ile Ser Val Asp Gly Val Val Asn
10 15 20
ggg gaa gtg cgc agc gac aaa gaa aag cag gcg ttc gag aag att acc 211
Gly Glu Val Arg Ser Asp Lys Glu Lys Gln Ala Phe Glu Lys Ile Thr
25 30 35
ggt gat gat ctg cgt cgt cgt gca gaa caa atc cgt aaa cgt cgt tta 259
Gly Asp Asp Leu Arg Arg Arg Ala Glu Gln Ile Arg Lys Arg Arg Leu
40 45 50
gca aaa gcg caa gaa gat gca cgt aag cgt aag cca ttg ttt ggt ttg 307
Ala Lys Ala Gln Glu Asp Ala Arg Lys Arg Lys Pro Leu Phe Gly Leu
55 60 65
ggt cgt atc gct gtc aca gta ctg gct ggc gcc gta ttt att ggt aca 355
Gly Arg Ile Ala Val Thr Val Leu Ala Gly Ala Val Phe Ile Gly Thr
70 75 80 85
acg ttg tgg atc agt acc acc tcg ggc ggc ttt gac gac aag gtg agt 403
Thr Leu Trp Ile Ser Thr Thr Ser Gly Gly Phe Asp Asp Lys Val Ser
90 95 100
gca aac aat gag cag att gtc act ctg cgt aat gaa gtc aac gac tta 451
Ala Asn Asn Glu Gln Ile Val Thr Leu Arg Asn Glu Val Asn Asp Leu
105 110 115
aaa aac act gca gag aca atg ccc aaa aaa gag act ctt gct agt caa 499
Lys Asn Thr Ala Glu Thr Met Pro Lys Lys Glu Thr Leu Ala Ser Gln
120 125 130
ttt gac gca gca acc tca cgt gca cag aat gtg gct gac tta cag aat 547
Phe Asp Ala Ala Thr Ser Arg Ala Gln Asn Val Ala Asp Leu Gln Asn
135 140 145
cag tta gcc ggc att att acc agt gtt gat gat gat gcc gcg acc gag 595
Gln Leu Ala Gly Ile Ile Thr Ser Val Asp Asp Asp Ala Ala Thr Glu
150 155 160 165
cag ttc aag aca att gtt gat gag ctg aaa ccg aaa ttc acg gtc tct 643
Gln Phe Lys Thr Ile Val Asp Glu Leu Lys Pro Lys Phe Thr Val Ser
170 175 180
gct ggt acg aca ggt gaa ttt cct gcg gcg ggt cgt tgg tac cag cca 691
Ala Gly Thr Thr Gly Glu Phe Pro Ala Ala Gly Arg Trp Tyr Gln Pro
185 190 195
caa gaa gtt gtt gtc gga gat aac aac cga ccc acc tgg gcg ccg atg 739
Gln Glu Val Val Val Gly Asp Asn Asn Arg Pro Thr Trp Ala Pro Met
200 205 210
ggg gct gag tcc tgg gga tgg act gtg acc cct aca ctg tcg atg tca 787
Gly Ala Glu Ser Trp Gly Trp Thr Val Thr Pro Thr Leu Ser Met Ser
215 220 225
gat acc gag cac gtt gtg gtg atg tgg gag gcc cgc ttg acc gga ggt 835
Asp Thr Glu His Val Val Val Met Trp Glu Ala Arg Leu Thr Gly Gly
230 235 240 245
gag cgt gac ggt gcg ctg ttg gcg tgg gtg aca gcc gat tac aac atc 883
Glu Arg Asp Gly Ala Leu Leu Ala Trp Val Thr Ala Asp Tyr Asn Ile
250 255 260
aac acc ggc gtg ttt agc tcg ctg gcg ttg gcg cat acc tat gaa gga 931
Asn Thr Gly Val Phe Ser Ser Leu Ala Leu Ala His Thr Tyr Glu Gly
265 270 275
cac cag cgt att ggt gca acc acc tca cca agt gaa ttt ggt gcc cac 979
His Gln Arg Ile Gly Ala Thr Thr Ser Pro Ser Glu Phe Gly Ala His
280 285 290
ggc aca aat gca gag gcg agc gct gca cgt gct ggc agt gaa ggc ggg 1027
Gly Thr Asn Ala Glu Ala Ser Ala Ala Arg Ala Gly Ser Glu Gly Gly
295 300 305
ggg ctt gag ggc gag gct att ttc gtt gat gag ctg cag cgg gca cta 1075
Gly Leu Glu Gly Glu Ala Ile Phe Val Asp Glu Leu Gln Arg Ala Leu
310 315 320 325
gag atc gct cag ctg aac aaa cag gag aac aga taatgacgac acgaaatgta 1128
Glu Ile Ala Gln Leu Asn Lys Gln Glu Asn Arg
330 335
gataaccaga 1138
<210>162
<211>336
<212>PRT
<213>谷氨酸棒状杆菌
<400>162
Met Ala Glu Asn Asn Gly Gly Ser Asp Asp Leu Lys Pro Ile Ser Val
1 5 10 15
Asp Gly Val Val Asn Gly Glu Val Arg Ser Asp Lys Glu Lys Gln Ala
20 25 30
Phe Glu Lys Ile Thr Gly Asp Asp Leu Arg Arg Arg Ala Glu Gln Ile
35 40 45
Arg Lys Arg Arg Leu Ala Lys Ala Gln Glu Asp Ala Arg Lys Arg Lys
50 55 60
Pro Leu Phe Gly Leu Gly Arg Ile Ala Val Thr Val Leu Ala Gly Ala
65 70 75 80
Val Phe Ile Gly Thr Thr Leu Trp Ile Ser Thr Thr Ser Gly Gly Phe
85 90 95
Asp Asp Lys Val Ser Ala Asn Asn Glu Gln Ile Val Thr Leu Arg Asn
100 105 110
Glu Val Asn Asp Leu Lys Asn Thr Ala Glu Thr Met Pro Lys Lys Glu
115 120 125
Thr Leu Ala Ser Gln Phe Asp Ala Ala Thr Ser Arg Ala Gln Asn Val
130 135 140
Ala Asp Leu Gln Asn Gln Leu Ala Gly Ile Ile Thr Ser Val Asp Asp
145 150 155 160
Asp Ala Ala Thr Glu Gln Phe Lys Thr Ile Val Asp Glu Leu Lys Pro
165 170 175
Lys Phe Thr Val Ser Ala Gly Thr Thr Gly Glu Phe Pro Ala Ala Gly
180 185 190
Arg Trp Tyr Gln Pro Gln Glu Val Val Val Gly Asp Asn Asn Arg Pro
195 200 205
Thr Trp Ala Pro Met Gly Ala Glu Ser Trp Gly Trp Thr Val Thr Pro
210 215 220
Thr Leu Ser Met Ser Asp Thr Glu His Val Val Val Met Trp Glu Ala
225 230 235 240
Arg Leu Thr Gly Gly Glu Arg Asp Gly Ala Leu Leu Ala Trp Val Thr
245 250 255
Ala Asp Tyr Asn Ile Asn Thr Gly Val Phe Ser Ser Leu Ala Leu Ala
260 265 270
His Thr Tyr Glu Gly His Gln Arg Ile Gly Ala Thr Thr Ser Pro Ser
275 280 285
Glu Phe Gly Ala His Gly Thr Asn Ala Glu Ala Ser Ala Ala Arg Ala
290 295 300
Gly Ser Glu Gly Gly Gly Leu Glu Gly Glu Ala Ile Phe Val Asp Glu
305 310 315 320
Leu Gln Arg Ala Leu Glu Ile Ala Gln Leu Asn Lys Gln Glu Asn Arg
325 330 335
<210>163
<211>1789
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1759)
<223>RXA01549
<400>163
cacagcttga tcaggcagtg gaaaatactc aggttgccca aggttccgat gctgttgaat 60
tccgtttcta gatttttttt attgattgtg agagtgcatt gtg gct aat gat ttt 115
Val Ala Asn Asp Phe
1 5
ata gaa cca aat gat gcc ccc gat gca cct gat aat ggt gac tcg gga 163
Ile Glu Pro Asn Asp Ala Pro Asp Ala Pro Asp Asn Gly Asp Ser Gly
10 15 20
agc tcg ggg ttt gtt gat gaa gca aaa gat aag acc cgc tca gct gca 211
Ser Ser Gly Phe Val Asp Glu Ala Lys Asp Lys Thr Arg Ser Ala Ala
25 30 35
caa ggc tta ggt tat ggc gca cta cgt gta ggt ttt ggt act gca gca 259
Gln Gly Leu Gly Tyr Gly Ala Leu Arg Val Gly Phe Gly Thr Ala Ala
40 45 50
ctt gca gcc aat ggt gct cgc tct atg ggt aat ttg gct aaa aac ctg 307
Leu Ala Ala Asn Gly Ala Arg Ser Met Gly Asn Leu Ala Lys Asn Leu
55 60 65
tgg aca cga atg atg ggg ctt gcc ctg tct gcc ggt act ggt atc tct 355
Trp Thr Arg Met Met Gly Leu Ala Leu Ser Ala Gly Thr Gly Ile Ser
70 75 80 85
gct gcc aca ggt ggc atg atc act gca cgt gta ggc gca atg ctg gca 403
Ala Ala Thr Gly Gly Met Ile Thr Ala Arg Val Gly Ala Met Leu Ala
90 95 100
ggt aca ggc tcg gtg ttg tcg tcg gtg acg act att gcg ttg att gtg 451
Gly Thr Gly Ser Val Leu Ser Ser Val Thr Thr Ile Ala Leu Ile Val
105 110 115
tcg atg att gtg gtg ccg gca ggt cga aaa gac ggc att atc gag ttg 499
Ser Met Ile Val Val Pro Ala Gly Arg Lys Asp Gly Ile Ile Glu Leu
120 125 130
tgt gag ccg cca gta acg gcc aac ccg ttt acc att gac gca ggt gat 547
Cys Glu Pro Pro Val Thr Ala Asn Pro Phe Thr Ile Asp Ala Gly Asp
135 140 145
atg act gag aac gcg aag ttg gtg tac ggt gcg ctg tcg tat ctt ggg 595
Met Thr Glu Asn Ala Lys Leu Val Tyr Gly Ala Leu Ser Tyr Leu Gly
150 155 160 165
atg aat gat caa aat atc gcc ggt gtc ttg ggc aac ttt gag aca gaa 643
Met Asn Asp Gln Asn Ile Ala Gly Val Leu Gly Asn Phe Glu Thr Glu
170 175 180
tca ggt atc gac ccg act gcg gtg gag ggt att ttt gat gaa cca aat 691
Ser Gly Ile Asp Pro Thr Ala Val Glu Gly Ile Phe Asp Glu Pro Asn
185 190 195
acc atc ggt cct cgg aag aga gct gcg tgg gat aag aac ttt gaa cct 739
Thr Ile Gly Pro Arg Lys Arg Ala Ala Trp Asp Lys Asn Phe Glu Pro
200 205 210
cag ccg atg ggt att ggg ctt ggg cag tgg aca gca ggg cgc aca cag 787
Gln Pro Met Gly Ile Gly Leu Gly Gln Trp Thr Ala Gly Arg Thr Gln
215 220 225
atg ttg cta gat ttt gcc gcc gat aga aac cga gac tgg cat tac atc 835
Met Leu Leu Asp Phe Ala Ala Asp Arg Asn Arg Asp Trp His Tyr Ile
230 235 240 245
gac gtg cag ctt gca ttt gct att agt ggc gat aat gaa agc gat cgc 883
Asp Val Gln Leu Ala Phe Ala Ile Ser Gly Asp Asn Glu Ser Asp Arg
250 255 260
aag gtt ttt ctg gaa atg gtt gat aat aaa aac tcc agt agc aat agc 931
Lys Val Phe Leu Glu Met Val Asp Asn Lys Asn Ser Ser Ser Asn Ser
265 270 275
ccc acc gca gcg tcc gag tac ttc ctg cgt gag tgg gag cgc ccg gca 979
Pro Thr Ala Ala Ser Glu Tyr Phe Leu Arg Glu Trp Glu Arg Pro Ala
280 285 290
gat gtc gca ggt aat gca ccc att cgt gca gag caa gca tct aag tgg 1027
Asp Val Ala Gly Asn Ala Pro Ile Arg Ala Glu Gln Ala Ser Lys Trp
295 300 305
tac gcg cag atg ggt ggt tgg caa aag aac tca acc ttg ggt gag tct 1075
Tyr Ala Gln Met Gly Gly Trp Gln Lys Asn Ser Thr Leu Gly Glu Ser
310 315 320 325
gtg atc gct atg gct gat ggt gca gca gca aaa tcc acg gcg cgt gat 1123
Val Ile Ala Met Ala Asp Gly Ala Ala Ala Lys Ser Thr Ala Arg Asp
330 335 340
gag cag gat gct ctt aat gat tgt cct gaa gag gac cgc acc tca ggt 1171
Glu Gln Asp Ala Leu Asn Asp Cys Pro Glu Glu Asp Arg Thr Ser Gly
345 350 355
gga aat acg tcc gct gca gaa gca atg gtg act att tca cat cct tat 1219
Gly Asn Thr Ser Ala Ala Glu Ala Met Val Thr Ile Ser His Pro Tyr
360 365 370
ctg gct gat tct cgt ggc aat gac ggt act gat att tac cgc tat atc 1267
Leu Ala Asp Ser Arg Gly Asn Asp Gly Thr Asp Ile Tyr Arg Tyr Ile
375 380 385
cat gat gaa gtg ctc acc ggt gat ccg tat tac gca tcc tgt gac cgt 1315
His Asp Glu Val Leu Thr Gly Asp Pro Tyr Tyr Ala Ser Cys Asp Arg
390 395 400 405
ggc gtt gct acc gct att cgc tgg tcg ggc acg gat gat acg ttc cca 1363
Gly Val Ala Thr Ala Ile Arg Trp Ser Gly Thr Asp Asp Thr Phe Pro
410 415 420
gca ggt cca aca gca gca cag tat gaa tac gtt gtg ggc aca gga tcg 1411
Ala Gly Pro Thr Ala Ala Gln Tyr Glu Tyr Val Val Gly Thr Gly Ser
425 430 435
ggc cgt tgg gag gaa atc ggc aat ctt gcc acc atg tca gag aac gat 1459
Gly Arg Trp Glu Glu Ile Gly Asn Leu Ala Thr Met Ser Glu Asn Asp
440 445 450
ttg tta cct ggc gat gtg ctt ctt ggt gca ccg aat cac gtt gcg atg 1507
Leu Leu Pro Gly Asp Val Leu Leu Gly Ala Pro Asn His Val Ala Met
455 460 465
tat gtc agc aat gag gtt gtt gtg gac atg ttg ggc cct ggt aat gca 1555
Tyr Val Ser Asn Glu Val Val Val Asp Met Leu Gly Pro Gly Asn Ala
470 475 480 485
gag cct aat gca gct att ggt cat gca tcg ttg aat gac cgc tca cct 1603
Glu Pro Asn Ala Ala Ile Gly His Ala Ser Leu Asn Asp Arg Ser Pro
490 495 500
ggt ctg gat acc ttg agt ctg gat gga tgg ggc gtg aac ttc aag gtg 1651
Gly Leu Asp Thr Leu Ser Leu Asp Gly Trp Gly Val Asn Phe Lys Val
505 510 515
ttc cgc aac acc cag gct gag aca aac tca gtg ttc tct ggt gta cag 1699
Phe Arg Asn Thr Gln Ala Glu Thr Asn Ser Val Phe Ser Gly Val Gln
520 525 530
att ccg gct ggt aaa gaa att ggc gaa atg act aac cca act cga act 1747
Ile Pro Ala Gly Lys Glu Ile Gly Glu Met Thr Asn Pro Thr Arg Thr
535 540 545
act cct gca gga taagtttttt tcatcgctgg gttggtgtga 1789
Thr Pro Ala Gly
550
<210>164
<211>553
<212>PRT
<213>谷氨酸棒状杆菌
<400>164
Val Ala Asn Asp Phe Ile Glu Pro Asn Asp Ala Pro Asp Ala Pro Asp
1 5 10 15
Asn Gly Asp Ser Gly Ser Ser Gly Phe Val Asp Glu Ala Lys Asp Lys
20 25 30
Thr Arg Ser Ala Ala Gln Gly Leu Gly Tyr Gly Ala Leu Arg Val Gly
35 40 45
Phe Gly Thr Ala Ala Leu Ala Ala Asn Gly Ala Arg Ser Met Gly Asn
50 55 60
Leu Ala Lys Asn Leu Trp Thr Arg Met Met Gly Leu Ala Leu Ser Ala
65 70 75 80
Gly Thr Gly Ile Ser Ala Ala Thr Gly Gly Met Ile Thr Ala Arg Val
85 90 95
Gly Ala Met Leu Ala Gly Thr Gly Ser Val Leu Ser Ser Val Thr Thr
100 105 110
Ile Ala Leu Ile Val Ser Met Ile Val Val Pro Ala Gly Arg Lys Asp
115 120 125
Gly Ile Ile Glu Leu Cys Glu Pro Pro Val Thr Ala Asn Pro Phe Thr
130 135 140
Ile Asp Ala Gly Asp Met Thr Glu Asn Ala Lys Leu Val Tyr Gly Ala
145 150 155 160
Leu Ser Tyr Leu Gly Met Asn Asp Gln Asn Ile Ala Gly Val Leu Gly
165 170 175
Asn Phe Glu Thr Glu Ser Gly Ile Asp Pro Thr Ala Val Glu Gly Ile
180 185 190
Phe Asp Glu Pro Asn Thr Ile Gly Pro Arg Lys Arg Ala Ala Trp Asp
195 200 205
Lys Asn Phe Glu Pro Gln Pro Met Gly Ile Gly Leu Gly Gln Trp Thr
210 215 220
Ala Gly Arg Thr Gln Met Leu Leu Asp Phe Ala Ala Asp Arg Asn Arg
225 230 235 240
Asp Trp His Tyr Ile Asp Val Gln Leu Ala Phe Ala Ile Ser Gly Asp
245 250 255
Asn Glu Ser Asp Arg Lys Val Phe Leu Glu Met Val Asp Asn Lys Asn
260 265 270
Ser Ser Ser Asn Ser Pro Thr Ala Ala Ser Glu Tyr Phe Leu Arg Glu
275 280 285
Trp Glu Arg Pro Ala Asp Val Ala Gly Asn Ala Pro Ile Arg Ala Glu
290 295 300
Gln Ala Ser Lys Trp Tyr Ala Gln Met Gly Gly Trp Gln Lys Asn Ser
305 310 315 320
Thr Leu Gly Glu Ser Val Ile Ala Met Ala Asp Gly Ala Ala Ala Lys
325 330 335
Ser Thr Ala Arg Asp Glu Gln Asp Ala Leu Asn Asp Cys Pro Glu Glu
340 345 350
Asp Arg Thr Ser Gly Gly Asn Thr Ser Ala Ala Glu Ala Met Val Thr
355 360 365
Ile Ser His Pro Tyr Leu Ala Asp Ser Arg Gly Asn Asp Gly Thr Asp
370 375 380
Ile Tyr Arg Tyr Ile His Asp Glu Val Leu Thr Gly Asp Pro Tyr Tyr
385 390 395 400
Ala Ser Cys Asp Arg Gly Val Ala Thr Ala Ile Arg Trp Ser Gly Thr
405 410 415
Asp Asp Thr Phe Pro Ala Gly Pro Thr Ala Ala Gln Tyr Glu Tyr Val
420 425 430
Val Gly Thr Gly Ser Gly Arg Trp Glu Glu Ile Gly Asn Leu Ala Thr
435 440 445
Met Ser Glu Asn Asp Leu Leu Pro Gly Asp Val Leu Leu Gly Ala Pro
450 455 460
Asn His Val Ala Met Tyr Val Ser Asn Glu Val Val Val Asp Met Leu
465 470 475 480
Gly Pro Gly Asn Ala Glu Pro Asn Ala Ala Ile Gly His Ala Ser Leu
485 490 495
Asn Asp Arg Ser Pro Gly Leu Asp Thr Leu Ser Leu Asp Gly Trp Gly
500 505 510
Val Asn Phe Lys Val Phe Arg Asn Thr Gln Ala Glu Thr Asn Ser Val
515 520 525
Phe Ser Gly Val Gln Ile Pro Ala Gly Lys Glu Ile Gly Glu Met Thr
530 535 540
Asn Pro Thr Arg Thr Thr Pro Ala Gly
545 550
<210>165
<211>2272
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2242)
<223>RXA01554
<400>165
tggcttaaac cttatttgta gttgtcaata aatatgaaat tccttagcaa cttgtttaaa 60
tagacgtata aacaagtttg aaaaaggaag gttatccatc ttg aaa aag cat gtg 115
Leu Lys Lys His Val
1 5
acc tca gcc gtt acc gcc gtg gtg acg gct ttt tca acg gct gcg ctt 163
Thr Ser Ala Val Thr Ala Val Val Thr Ala Phe Ser Thr Ala Ala Leu
10 15 20
ggt tta agt att gcc gtt tct cct gct gtt gcc caa gtg gct aat cca 211
Gly Leu Ser Ile Ala Val Ser Pro Ala Val Ala Gln Val Ala Asn Pro
25 30 35
gct cca gat ctt tct gcg ccg tat aca tgg gtg gaa gag ttt gat tcc 259
Ala Pro Asp Leu Ser Ala Pro Tyr Thr Trp Val Glu Glu Phe Asp Ser
40 45 50
gag gat gct ctc aaa ggg tgg aac att ttc cgc cag cca gat tat ggc 307
Glu Asp Ala Leu Lys Gly Trp Asn Ile Phe Arg Gln Pro Asp Tyr Gly
55 60 65
agc gac aaa gtt ctc tat acc gaa gat gct tta agt atc gaa gat ggc 355
Ser Asp Lys Val Leu Tyr Thr Glu Asp Ala Leu Ser Ile Glu Asp Gly
70 75 80 85
aag ctc acc atc acc act cag cgc cac tgc gtt gac gaa gac ttc gcg 403
Lys Leu Thr Ile Thr Thr Gln Arg His Cys Val Asp Glu Asp Phe Ala
90 95 100
atc agt gat cct gtc aac cgc gga aag ctc aat gac agc acc gcg caa 451
Ile Ser Asp Pro Val Asn Arg Gly Lys Leu Asn Asp Ser Thr Ala Gln
105 110 115
gtt gaa cct tgt gct cca ggt cag ttt gaa aag ttc acc agt gcg cgc 499
Val Glu Pro Cys Ala Pro Gly Gln Phe Glu Lys Phe Thr Ser Ala Arg
120 125 130
atc gtc act ccg aaa att gct cgt gga gag ttc gac ctt tct gtc act 547
Ile Val Thr Pro Lys Ile Ala Arg Gly Glu Phe Asp Leu Ser Val Thr
135 140 145
gca act ctt aac acc ggt ggc gtc gaa ggt gtc cga agt gcc att tgg 595
Ala Thr Leu Asn Thr Gly Gly Val Glu Gly Val Arg Ser Ala Ile Trp
150 155 160 165
atg caa aac ggt gaa cag gcg tgt tcc tca gca acc aac aat ggc ctc 643
Met Gln Asn Gly Glu Gln Ala Cys Ser Ser Ala Thr Asn Asn Gly Leu
170 175 180
tac gga gaa cta gac ctg gta gag cac ttt tct tac gat ctt cgc tcg 691
Tyr Gly Glu Leu Asp Leu Val Glu His Phe Ser Tyr Asp Leu Arg Ser
185 190 195
cca tgg tct cca tca aac acc cac ttg ggt tgt gat cct gaa agt gtc 739
Pro Trp Ser Pro Ser Asn Thr His Leu Gly Cys Asp Pro Glu Ser Val
200 205 210
aac ggc acc aac cgt gca cct cgt gaa ctt aaa cta gat gag tca ctc 787
Asn Gly Thr Asn Arg Ala Pro Arg Glu Leu Lys Leu Asp Glu Ser Leu
215 220 225
gat ggc gtg gag cac acc tgg act gtg agc acc acc cgc gac ggc gtt 835
Asp Gly Val Glu His Thr Trp Thr Val Ser Thr Thr Arg Asp Gly Val
230 235 240 245
gag tac ttc att gat gat gag gcg att aac cgc cag tca tgg cgc aac 883
Glu Tyr Phe Ile Asp Asp Glu Ala Ile Asn Arg Gln Ser Trp Arg Asn
250 255 260
gat gtc act ttg ggg cat gcc gaa att gat gat ttc ggg atc tcc gcg 931
Asp Val Thr Leu Gly His Ala Glu Ile Asp Asp Phe Gly Ile Ser Ala
265 270 275
cag acg ttt gat gag atc gtc gac cgc gaa tgg act ctc act ctt aat 979
Gln Thr Phe Asp Glu Ile Val Asp Arg Glu Trp Thr Leu Thr Leu Asn
280 285 290
caa aag gta gaa agc gcc gac tgg gca aaa cca cgt tcc tct gag gaa 1027
Gln Lys Val Glu Ser Ala Asp Trp Ala Lys Pro Arg Ser Ser Glu Glu
295 300 305
gat ttc cca gtc cgg tcc atg gtg att gac cgc atc gag gtc acc gga 1075
Asp Phe Pro Val Arg Ser Met Val Ile Asp Arg Ile Glu Val Thr Gly
310 315 320 325
tct ccc gca gta tct gaa gac acc ccc atg cca gat acc acc cag ctt 1123
Ser Pro Ala Val Ser Glu Asp Thr Pro Met Pro Asp Thr Thr Gln Leu
330 335 340
ttg acc caa gac act ctg gaa tac ctc ggt cgc atg cca gtg ctg gaa 1171
Leu Thr Gln Asp Thr Leu Glu Tyr Leu Gly Arg Met Pro Val Leu Glu
345 350 355
cgc tac gag cca gca agt gct gat ttt gcc gat ggc cgc agg cct tcc 1219
Arg Tyr Glu Pro Ala Ser Ala Asp Phe Ala Asp Gly Arg Arg Pro Ser
360 365 370
tgg aac tac ttc aat ttg aag gaa tcg tgg cag aat cca gaa ctc gag 1267
Trp Asn Tyr Phe Asn Leu Lys Glu Ser Trp Gln Asn Pro Glu Leu Glu
375 380 385
caa cgc cca gaa gct gtc gaa ttc gtt gat gga cgc atg gat atc gtg 1315
Gln Arg Pro Glu Ala Val Glu Phe Val Asp Gly Arg Met Asp Ile Val
390 395 400 405
acc cgt cgc cac tgt ctg gcc acc act gat gac atc gcc act ccg gaa 1363
Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp Ile Ala Thr Pro Glu
410 415 420
aac gca cag gag gaa ccg tgc gcg ccg ggt gag gtg aca cgc tac agc 1411
Asn Ala Gln Glu Glu Pro Cys Ala Pro Gly Glu Val Thr Arg Tyr Ser
425 430 435
tca gcg cgt gtc cac ctt cca gag atc ccc gcc ggc aac ttc cgg ctc 1459
Ser Ala Arg Val His Leu Pro Glu Ile Pro Ala Gly Asn Phe Arg Leu
440 445 450
acc gtg cgc gca cgg gcg cag tcc gaa gag ctt gtc gac ggc gtc cgc 1507
Thr Val Arg Ala Arg Ala Gln Ser Glu Glu Leu Val Asp Gly Val Arg
455 460 465
ccc gct atc tgg atg cag aac aat acc aac ttc tgt gct gac aac gat 1555
Pro Ala Ile Trp Met Gln Asn Asn Thr Asn Phe Cys Ala Asp Asn Asp
470 475 480 485
gga cgc cct tat ggt gaa ctg gat att act gag ttc tac agc tct cgt 1603
Gly Arg Pro Tyr Gly Glu Leu Asp Ile Thr Glu Phe Tyr Ser Ser Arg
490 495 500
gtg aac acc cag tac tcg gca gta cac ctt gga tgt gct ggc aac cgc 1651
Val Asn Thr Gln Tyr Set Ala Val His Leu Gly Cys Ala Gly Asn Arg
505 510 515
cca gag atg aag ctt cgc caa atg gaa atg gaa gag tcc atg ttt ggg 1699
Pro Glu Met Lys Leu Arg Gln Met Glu Met Glu Glu Ser Met Phe Gly
520 525 530
gat tgg cat gac tgg ggc gtc gaa gtc ttc gac ggc cag atc gta ttc 1747
Asp Trp His Asp Trp Gly Val Glu Val Phe Asp Gly Gln Ile Val Phe
535 540 545
acc att gac ggc aag gca gta act tcc tct ggc aaa gat gtc ttt ggc 1795
Thr Ile Asp Gly Lys Ala Val Thr Ser Ser Gly Lys Asp Val Phe Gly
550 555 560 565
aac tct gtt acc cca gcc gct gca cct ctt cgc ccc gcg cac ttc aag 1843
Asn Ser Val Thr Pro Ala Ala Ala Pro Leu Arg Pro Ala His Phe Lys
570 575 580
ttg tcg gaa gag gaa tac cgt gaa gtc atc ggg cag cct tgg cac ctt 1891
Leu Ser Glu Glu Glu Tyr Arg Glu Val Ile Gly Gln Pro Trp His Leu
585 590 595
att ttg aac acc atg gtg gag cag tct ggc aaa gac agc tgg att aca 1939
Ile Leu Asn Thr Met Val Glu Gln Ser Gly Lys Asp Ser Trp Ile Thr
600 605 610
gcg gtc gac aat aac gag gcg ttc cca gaa cac cgc ttc caa att gac 1987
Ala Val Asp Asn Asn Glu Ala Phe Pro Glu His Arg Phe Gln Ile Asp
615 620 625
cat gtg gca gta gat atc gag tct gac tct gtg gac aat gta tgg cct 2035
His Val Ala Val Asp Ile Glu Ser Asp Ser Val Asp Asn Val Trp Pro
630 635 640 645
gac gct gcg aat gaa atc cca gac aat gtt ggt att gaa gac tct gat 2083
Asp Ala Ala Asn Glu Ile Pro Asp Asn Val Gly Ile Glu Asp Ser Asp
650 655 660
gat ggc agc gac ctg gag gtt ggt tcg acc gga agc tct aca gct gag 2131
Asp Gly Ser Asp Leu Glu Val Gly Ser Thr Gly Ser Ser Thr Ala Glu
665 670 675
acc gtg agc tgg atc tcg ttg ttc acc gcg ttg agc tcg ctg gtc ttc 2179
Thr Val Ser Trp Ile Ser Leu Phe Thr Ala Leu Ser Ser Leu Val Phe
680 685 690
aca ctg gct ctc aat caa gaa gca ttg cag aat ttg att aat cag ttc 2227
Thr Leu Ala Leu Asn Gln Glu Ala Leu Gln Asn Leu Ile Asn Gln Phe
695 700 705
atg aga cag ttc aag taatccttgt gggcgctttg tctacttcac 2272
Met Arg Gln Phe Lys
710
<210>166
<211>714
<212>PRT
<213>谷氨酸棒状杆菌
<400>166
Leu Lys Lys His Val Thr Ser Ala Val Thr Ala Val Val Thr Ala Phe
1 5 10 15
Ser Thr Ala Ala Leu Gly Leu Ser Ile Ala Val Ser Pro Ala Val Ala
20 25 30
Gln Val Ala Asn Pro Ala Pro Asp Leu Ser Ala Pro Tyr Thr Trp Val
35 40 45
Glu Glu Phe Asp Ser Glu Asp Ala Leu Lys Gly Trp Asn Ile Phe Arg
50 55 60
Gln Pro Asp Tyr Gly Ser Asp Lys Val Leu Tyr Thr Glu Asp Ala Leu
65 70 75 80
Ser Ile Glu Asp Gly Lys Leu Thr Ile Thr Thr Gln Arg His Cys Val
85 90 95
Asp Glu Asp Phe Ala Ile Ser Asp Pro Val Asn Arg Gly Lys Leu Asn
100 105 110
Asp Ser Thr Ala Gln Val Glu Pro Cys Ala Pro Gly Gln Phe Glu Lys
115 120 125
Phe Thr Ser Ala Arg Ile Val Thr Pro Lys Ile Ala Arg Gly Glu Phe
130 135 140
Asp Leu Ser Val Thr Ala Thr Leu Asn Thr Gly Gly Val Glu Gly Val
145 150 155 160
Arg Ser Ala Ile Trp Met Gln Asn Gly Glu Gln Ala Cys Ser Ser Ala
165 170 175
Thr Asn Asn Gly Leu Tyr Gly Glu Leu Asp Leu Val Glu His Phe Ser
180 185 190
Tyr Asp Leu Arg Ser Pro Trp Ser Pro Ser Asn Thr His Leu Gly Cys
195 200 205
Asp Pro Glu Ser Val Asn Gly Thr Asn Arg Ala Pro Arg Glu Leu Lys
210 215 220
Leu Asp Glu Ser Leu Asp Gly Val Glu His Thr Trp Thr Val Ser Thr
225 230 235 240
Thr Arg Asp Gly Val Glu Tyr Phe Ile Asp Asp Glu Ala Ile Asn Arg
245 250 255
Gln Ser Trp Arg Asn Asp Val Thr Leu Gly His Ala Glu Ile Asp Asp
260 265 270
Phe Gly Ile Ser Ala Gln Thr Phe Asp Glu Ile Val Asp Arg Glu Trp
275 280 285
Thr Leu Thr Leu Asn Gln Lys Val Glu Ser Ala Asp Trp Ala Lys Pro
290 295 300
Arg Ser Ser Glu Glu Asp Phe Pro Val Arg Ser Met Val Ile Asp Arg
305 310 315 320
Ile Glu Val Thr Gly Ser Pro Ala Val Ser Glu Asp Thr Pro Met Pro
325 330 335
Asp Thr Thr Gln Leu Leu Thr Gln Asp Thr Leu Glu Tyr Leu Gly Arg
340 345 350
Met Pro Val Leu Glu Arg Tyr Glu Pro Ala Ser Ala Asp Phe Ala Asp
355 360 365
Gly Arg Arg Pro Ser Trp Asn Tyr Phe Asn Leu Lys Glu Ser Trp Gln
370 375 380
Asn Pro Glu Leu Glu Gln Arg Pro Glu Ala Val Glu Phe Val Asp Gly
385 390 395 400
Arg Met Asp Ile Val Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp
405 410 415
Ile Ala Thr Pro Glu Asn Ala Gln Glu Glu Pro Cys Ala Pro Gly Glu
420 425 430
Val Thr Arg Tyr Ser Ser Ala Arg Val His Leu Pro Glu Ile Pro Ala
435 440 445
Gly Asn Phe Arg Leu Thr Val Arg Ala Arg Ala Gln Ser Glu Glu Leu
450 455 460
Val Asp Gly Val Arg Pro Ala Ile Trp Met Gln Asn Asn Thr Asn Phe
465 470 475 480
Cys Ala Asp Asn Asp Gly Arg Pro Tyr Gly Glu Leu Asp Ile Thr Glu
485 490 495
Phe Tyr Ser Ser Arg Val Asn Thr Gln Tyr Ser Ala Val His Leu Gly
500 505 510
Cys Ala Gly Asn Arg Pro Glu Met Lys Leu Arg Gln Met Glu Met Glu
515 520 525
Glu Ser Met Phe Gly Asp Trp His Asp Trp Gly Val Glu Val Phe Asp
530 535 540
Gly Gln Ile Val Phe Thr Ile Asp Gly Lys Ala Val Thr Ser Ser Gly
545 550 555 560
Lys Asp Val Phe Gly Asn Ser Val Thr Pro Ala Ala Ala Pro Leu Arg
565 570 575
Pro Ala His Phe Lys Leu Ser Glu Glu Glu Tyr Arg Glu Val Ile Gly
580 585 590
Gln Pro Trp His Leu Ile Leu Asn Thr Met Val Glu Gln Ser Gly Lys
595 600 605
Asp Ser Trp Ile Thr Ala Val Asp Asn Asn Glu Ala Phe Pro Glu His
610 615 620
Arg Phe Gln Ile Asp His Val Ala Val Asp Ile Glu Ser Asp Ser Val
625 630 635 640
Asp Asn Val Trp Pro Asp Ala Ala Asn Glu Ile Pro Asp Asn Val Gly
645 650 655
Ile Glu Asp Ser Asp Asp Gly Ser Asp Leu Glu Val Gly Ser Thr Gly
660 665 670
Ser Ser Thr Ala Glu Thr Val Ser Trp Ile Ser Leu Phe Thr Ala Leu
675 680 685
Ser Ser Leu Val Phe Thr Leu Ala Leu Asn Gln Glu Ala Leu Gln Asn
690 695 700
Leu Ile Asn Gln Phe Met Arg Gln Phe Lys
705 710
<210>167
<211>1165
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1135)
<223>RXA01574
<400>167
aaaaatccgc acaccccaca ttgctcaaag gaattgacaa ggcgcttcgc aaggccgagt 60
aaggtgggca acgagtcggt gagagtatga ggaatatttg atg agc aac aaa cgc 115
Met Ser Asn Lys Arg
1 5
atc ggt gta gtg atc gtt tcc tac gga cac gaa caa gat gtt gcc aac 163
Ile Gly Val Val Ile Val Ser Tyr Gly His Glu Gln Asp Val Ala Asn
10 15 20
ctg gta gac aca ttt gca gat cag ctg aaa act ggt gac cgc gta gtt 211
Leu Val Asp Thr Phe Ala Asp Gln Leu Lys Thr Gly Asp Arg Val Val
25 30 35
gtc gtg gac aac cgc aaa cct tgg gtg tta aaa gac gcc gtg ggg gag 259
Val Val Asp Asn Arg Lys Pro Trp Val Leu Lys Asp Ala Val Gly Glu
40 45 50
cgc ctg gaa aaa cac ggc gca gag atc atc aac cac gac aac ggt ggt 307
Arg Leu Glu Lys His Gly Ala Glu Ile Ile Asn His Asp Asn Gly Gly
55 60 65
ttc gcc gct ggc tgc aac gtg ggc gca gcg cac atc gtg gat gac gtt 355
Phe Ala Ala Gly Cys Asn Val Gly Ala Ala His Ile Val Asp Asp Val
70 75 80 85
gac ctg ctg ttc ttc ctc aac ccc gac aca gtg atc gac gat ccc acc 403
Asp Leu Leu Phe Phe Leu Asn Pro Asp Thr Val Ile Asp Asp Pro Thr
90 95 100
ctg ttc aat tcg ctg aga cgc gtc gat gaa cag tgg gca gca ttc atg 451
Leu Phe Asn Ser Leu Arg Arg Val Asp Glu Gln Trp Ala Ala Phe Met
105 110 115
ccg tac ctg ctg ctt cct gac agc acc att aac tcc gca ggc aac gcc 499
Pro Tyr Leu Leu Leu Pro Asp Ser Thr Ile Asn Ser Ala Gly Asn Ala
120 125 130
ctg cat att tcc gga ctg tcg tgg gtg act ggt ctg gat gaa aaa cca 547
Leu His Ile Ser Gly Leu Ser Trp Val Thr Gly Leu Asp Glu Lys Pro
135 140 145
gtt gaa ggc tca tct gaa gtt acc gat att tcc att gcc tct ggc gcc 595
Val Glu Gly Ser Ser Glu Val Thr Asp Ile Ser Ile Ala Ser Gly Ala
150 155 160 165
tgc ctt gcc gtg cgc gtg gac tgg tgg aaa cgc ctc ggt ggc atg gaa 643
Cys Leu Ala Val Arg Val Asp Trp Trp Lys Arg Leu Gly Gly Met Glu
170 175 180
gaa ctg tat ttc atg tac cac gaa gac act gac ttc tcc gcc cgc ttg 691
Glu Leu Tyr Phe Met Tyr His Glu Asp Thr Asp Phe Ser Ala Arg Leu
185 190 195
ctg ctg gcc ggc ggt cga att ggt ctc ctg cat tcc gcg tat gtc acc 739
Leu Leu Ala Gly Gly Arg Ile Gly Leu Leu His Ser Ala Tyr Val Thr
200 205 210
cac cat tac gac tac gcc aag ggt gac tac aag tgg att tac atc gaa 787
His His Tyr Asp Tyr Ala Lys Gly Asp Tyr Lys Trp Ile Tyr Ile Glu
215 220 225
cga aac cga cac gtt ttg ctg ctc agc gtg ctg ccg ctt cca ttg ctg 835
Arg Asn Arg His Val Leu Leu Leu Ser Val Leu Pro Leu Pro Leu Leu
230 235 240 245
ttc gtg ctg atc ccg cag atc ctc ggt gtg aac ctg gga ctg tgg gcg 883
Phe Val Leu Ile Pro Gln Ile Leu Gly Val Asn Leu Gly Leu Trp Ala
250 255 260
att gcc gca aag gaa aag agg gtc gga ctc aag gtg aag tcc ctt cgc 931
Ile Ala Ala Lys Glu Lys Arg Val Gly Leu Lys Val Lys Ser Leu Arg
265 270 275
ctc ctg atc cgc gat cta cca gcg att ttc aaa ctg cgt agg agc acg 979
Leu Leu Ile Arg Asp Leu Pro Ala Ile Phe Lys Leu Arg Arg Ser Thr
280 285 290
cag gag ctt gcc gaa ctc aca cca tcg caa tat ctg gca aaa atg gaa 1027
Gln Glu Leu Ala Glu Leu Thr Pro Ser Gln Tyr Leu Ala Lys Met Glu
295 300 305
tgg cgc cta gac aat ccc aac cta ggc aac att gga tcc aac aag att 1075
Trp Arg Leu Asp Asn Pro Asn Leu Gly Asn Ile Gly Ser Asn Lys Ile
310 315 320 325
gtt gcg act gga tat aag acc tat tac aag ttg tgt atg agt atc ctg 1123
Val Ala Thr Gly Tyr Lys Thr Tyr Tyr Lys Leu Cys Met Ser Ile Leu
330 335 340
aaa ttg ctc gct taacacccca taaagagggt gaagatttaa 1165
Lys Leu Leu Ala
345
<210>168
<211>345
<212>PRT
<213>谷氨酸棒状杆菌
<400>168
Met Ser Asn Lys Arg Ile Gly Val Val Ile Val Ser Tyr Gly His Glu
1 5 10 15
Gln Asp Val Ala Asn Leu Val Asp Thr Phe Ala Asp Gln Leu Lys Thr
20 25 30
Gly Asp Arg Val Val Val Val Asp Asn Arg Lys Pro Trp Val Leu Lys
35 40 45
Asp Ala Val Gly Glu Arg Leu Glu Lys His Gly Ala Glu Ile Ile Asn
50 55 60
His Asp Asn Gly Gly Phe Ala Ala Gly Cys Asn Val Gly Ala Ala His
65 70 75 80
Ile Val Asp Asp Val Asp Leu Leu Phe Phe Leu Asr Pro Asp Thr Val
85 90 95
Ile Asp Asp Pro Thr Leu Phe Asn Ser Leu Arg Arg Val Asp Glu Gln
100 105 110
Trp Ala Ala Phe Met Pro Tyr Leu Leu Leu Pro Asp Ser Thr Ile Asn
115 120 125
Ser Ala Gly Asn Ala Leu His Ile Ser Gly Leu Ser Trp Val Thr Gly
130 135 140
Leu Asp Glu Lys Pro Val Glu Gly Ser Ser Glu Val Thr Asp Ile Ser
145 150 155 160
Ile Ala Ser Gly Ala Cys Leu Ala Val Arg Val Asp Trp Trp Lys Arg
165 170 175
Leu Gly Gly Met Glu Glu Leu Tyr Phe Met Tyr His Glu Asp Thr Asp
180 185 190
Phe Ser Ala Arg Leu Leu Leu Ala Gly Gly Arg Ile Gly Leu Leu His
195 200 205
Ser Ala Tyr Val Thr His His Tyr Asp Tyr Ala Lys Gly Asp Tyr Lys
210 215 220
Trp Ile Tyr Ile Glu Arg Asn Arg His Val Leu Leu Leu Ser Val Leu
225 230 235 240
Pro Leu Pro Leu Leu Phe Val Leu Ile Pro Gln Ile Leu Gly Val Asn
245 250 255
Leu Gly Leu Trp Ala Ile Ala Ala Lys Glu Lys Arg Val Gly Leu Lys
260 265 270
Val Lys Ser Leu Arg Leu Leu Ile Arg Asp Leu Pro Ala Ile Phe Lys
275 280 285
Leu Arg Arg Ser Thr Gln Glu Leu Ala Glu Leu Thr Pro Ser Gln Tyr
290 295 300
Leu Ala Lys Met Glu Trp Arg Leu Asp Asn Pro Asn Leu Gly Asn Ile
305 310 315 320
Gly Ser Asn Lys Ile Val Ala Thr Gly Tyr Lys Thr Tyr Tyr Lys Leu
325 330 335
Cys Met Ser Ile Leu Lys Leu Leu Ala
340 345
<210>169
<211>757
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(727)
<223>RXA01585
<400>169
tgcagactgc tgcgccccgt tccatcagca aggtggagcg caaggtgctg accgctgtcg 60
ctactgatga ggtgcagtcg caggaagctc tagaaaagta gtg gtt gag act ctt 115
Val Val Glu Thr Leu
1 5
tct atc acc tca ggt gcg gct ggc ctg cag gcg ctt gtt gcg cgt gcg 163
Ser Ile Thr Ser Gly Ala Ala Gly Leu Gln Ala Leu Val Ala Arg Ala
10 15 20
gtt ggt ttg gat gag ttg gcg agc gcg cgt ttc cgc cag ctc acg cca 211
Val Gly Leu Asp Glu Leu Ala Ser Ala Arg Phe Arg Gln Leu Thr Pro
25 30 35
gag gtc gtt gat gtt ttt gtc acc act ccg ttc aac gtg acg gcg tcg 259
Glu Val Val Asp Val Phe Val Thr Thr Pro Phe Asn Val Thr Ala Ser
40 45 50
cgc cgg gta ttt ggt gtg gcg ggt cgc gat ggc gct gca gtt ggc gcg 307
Arg Arg Val Phe Gly Val Ala Gly Arg Asp Gly Ala Ala Val Gly Ala
55 60 65
aag gat ttg ctg cag gca ctc agc gag ggc cgg gaa gac gta ggc acc 355
Lys Asp Leu Leu Gln Ala Leu Ser Glu Gly Arg Glu Asp Val Gly Thr
70 75 80 85
tcc cgc gac gcc agc tgg ccg ggc tca ttg ccg cca gcc acg ggc ttt 403
Ser Arg Asp Ala Ser Trp Pro Gly Ser Leu Pro Pro Ala Thr Gly Phe
90 95 100
acg ctt gtc gac gaa ctc ccc acc cac gtg gtg cgc act ttg gct gat 451
Thr Leu Val Asp Glu Leu Pro Thr His Val Val Arg Thr Leu Ala Asp
105 110 115
cag ggt cag gca ttg gct cgc cag ttc tcc ggc ccg ctt ggc cct ccg 499
Gln Gly Gln Ala Leu Ala Arg Gln Phe Ser Gly Pro Leu Gly Pro Pro
120 125 130
gct tcg ttg atg gat cag gaa gtt att tca gca gaa ggc aat ggc aag 547
Ala Ser Leu Met Asp Gln Glu Val Ile Ser Ala Glu Gly Asn Gly Lys
135 140 145
aag gcc gga atc ccg atg cgc acg gta ttt acc tgc act tcg ctt ggt 595
Lys Ala Gly Ile Pro Met Arg Thr Val Phe Thr Cys Thr Ser Leu Gly
150 155 160 165
ttg atc cca ggt ttt gag gca gcc aat gat gtg ccg agg cac ctg cgg 643
Leu Ile Pro Gly Phe Glu Ala Ala Asn Asp Val Pro Arg His Leu Arg
170 175 180
gta tcg gtg aat ggt cgt tgg acc cgc gtt gat gct ccc tat ggc agt 691
Val Ser Val Asn Gly Arg Trp Thr Arg Val Asp Ala Pro Tyr Gly Ser
185 190 195
gtt tat cac tcg tca ggt ttg ggt ctg agc gtt ttc taaagcaaga 737
Val Tyr His Ser Ser Gly Leu Gly Leu Ser Val Phe
200 205
cccagcctgc aagggccgca 757
<210>170
<211>209
<212>PRT
<213>谷氨酸棒状杆菌
<400>170
Val Val Glu Thr Leu Ser Ile Thr Ser Gly Ala Ala Gly Leu Gln Ala
1 5 10 15
Leu Val Ala Arg Ala Val Gly Leu Asp Glu Leu Ala Ser Ala Arg Phe
20 25 30
Arg Gln Leu Thr Pro Glu Val Val Asp Val Phe Val Thr Thr Pro Phe
35 40 45
Asn Val Thr Ala Ser Arg Arg Val Phe Gly Val Ala Gly Arg Asp Gly
50 55 60
Ala Ala Val Gly Ala Lys Asp Leu Leu Gln Ala Leu Ser Glu Gly Arg
65 70 75 80
Glu Asp Val Gly Thr Ser Arg Asp Ala Ser Trp Pro Gly Ser Leu Pro
85 90 95
Pro Ala Thr Gly Phe Thr Leu Val Asp Glu Leu Pro Thr His Val Val
100 105 110
Arg Thr Leu Ala Asp Gln Gly Gln Ala Leu Ala Arg Gln Phe Ser Gly
115 120 125
Pro Leu Gly Pro Pro Ala Ser Leu Met Asp Gln Glu Val Ile Ser Ala
130 135 140
Glu Gly Asn Gly Lys Lys Ala Gly Ile Pro Met Arg Thr Val Phe Thr
145 150 155 160
Cys Thr Ser Leu Gly Leu Ile Pro Gly Phe Glu Ala Ala Asn Asp Val
165 170 175
Pro Arg His Leu Arg Val Ser Val Asn Gly Arg Trp Thr Arg Val Asp
180 185 190
Ala Pro Tyr Gly Ser Val Tyr His Ser Ser Gly Leu Gly Leu Ser Val
195 200 205
Phe
<210>171
<211>6592
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(6562)
<223>RXA01590
<400>171
tccgcatgtc cattagtgat atgacaaaag aataaagaaa agggaggact ttagtatgtc 60
caaaacttta tttcgacacg cttttgaacg gtcgcttgag atg gcg cgc tca gag 115
Met Ala Arg Ser Glu
1 5
tgg ggt caa aaa gat cac tgg ggc aaa gct cca ctg ggt gat cac gat 163
Trp Gly Gln Lys Asp His Trp Gly Lys Ala Pro Leu Gly Asp His Asp
10 15 20
gct gtg cgt atc tat agg caa gca gaa gcc ttg atg aaa cag cgc gca 211
Ala Val Arg Ile Tyr Arg Gln Ala Glu Ala Leu Met Lys Gln Arg Ala
25 30 35
gca aac aat aag ggc tta gac ctc aat gat gaa gcg acc tat ggc gcc 259
Ala Asn Asn Lys Gly Leu Asp Leu Asn Asp Glu Ala Thr Tyr Gly Ala
40 45 50
atc atg ttt gcg tct ttc gat gat gcc ttc gcc gct gat gcc gaa gct 307
Ile Met Phe Ala Ser Phe Asp Asp Ala Phe Ala Ala Asp Ala Glu Ala
55 60 65
cat ggc tgt gta cac cga cca cag ttc gcg aag gta act agg cga aat 355
His Gly Cys Val His Arg Pro Gln Phe Ala Lys Val Thr Arg Arg Asn
70 75 80 85
att ctt aat tca ccg caa tac acg gcg att aag tca tct tat gac gcg 403
Ile Leu Asn Ser Pro Gln Tyr Thr Ala Ile Lys Ser Ser Tyr Asp Ala
90 95 100
ctg gtg cag cag gat ctc gtg gcc caa cag cag cag gca caa gcg cta 451
Leu Val Gln Gln Asp Leu Val Ala Gln Gln Gln Gln Ala Gln Ala Leu
105 110 115
cag caa gct gct tat gag caa gag cag gca gct att ttc cag gca cgt 499
Gln Gln Ala Ala Tyr Glu Gln Glu Gln Ala Ala Ile Phe Gln Ala Arg
120 125 130
gat caa gca aca ttc cag gct gaa caa acg ctc gcg caa gag tcc gtt 547
Asp Gln Ala Thr Phe Gln Ala Glu Gln Thr Leu Ala Gln Glu Ser Val
135 140 145
cgc caa tca gag ggt gtg ggt tta cct gtc att gat ccg atg gag agc 595
Arg Gln Ser Glu Gly Val Gly Leu Pro Val Ile Asp Pro Met Glu Ser
150 155 160 165
tat ttc cgg gag tac gcc ttc tat gaa ggt gtc gag atg ttt ggt acc 643
Tyr Phe Arg Glu Tyr Ala Phe Tyr Glu Gly Val Glu Met Phe Gly Thr
170 175 180
tgg ggt acg cac gtt gat gct ggt gtg tcg cag ttt agc acc tct gat 691
Trp Gly Thr His Val Asp Ala Gly Val Ser Gln Phe Ser Thr Ser Asp
185 190 195
ggc cgt gcg atc cga gcg ctt gtt gat gag cat ctc gca acc tat gaa 739
Gly Arg Ala Ile Arg Ala Leu Val Asp Glu His Leu Ala Thr Tyr Glu
200 205 210
cca gta gag cag cgg ggg agt act cag gca ttc ttc gcc tct gtc aat 787
Pro Val Glu Gln Arg Gly Ser Thr Gln Ala Phe Phe Ala Ser Val Asn
215 220 225
gct gcc ttt gct gag gtg gca cct cac gca gca ccc atg ttt agt gcg 835
Ala Ala Phe Ala Glu Val Ala Pro His Ala Ala Pro Met Phe Ser Ala
230 235 240 245
att gct cgc gag ggt gag ggt tca aag ggg tac cag gcg ctg cgt cac 883
Ile Ala Arg Glu Gly Glu Gly Ser Lys Gly Tyr Gln Ala Leu Arg His
250 255 260
cgt ttc gac aag atg gtc ggc act gca att gga cct aat ggt ttg cct 931
Arg Phe Asp Lys Met Val Gly Thr Ala Ile Gly Pro Asn Gly Leu Pro
265 270 275
gag gga cct gat ctg ggt ggt cgt cgt tta cct att tcg cct tat gat 979
Glu Gly Pro Asp Leu Gly Gly Arg Arg Leu Pro Ile Ser Pro Tyr Asp
280 285 290
cca cgc tgg tca gac cgc gaa gta gtc cgt gtg cac ggt acc aag ctg 1027
Pro Arg Trp Ser Asp Arg Glu Val Val Arg Val His Gly Thr Lys Leu
295 300 305
cta tca ctg tcc gaa gag agt gtt ggc atg atc agg gac ctt gat cta 1075
Leu Ser Leu Ser Glu Glu Ser Val Gly Met Ile Arg Asp Leu Asp Leu
310 315 320 325
gca acg atc cac cta caa gat aat gat gtt tac gag ggc act cat cga 1123
Ala Thr Ile His Leu Gln Asp Asn Asp Val Tyr Glu Gly Thr His Arg
330 335 340
gaa gac gac act aaa ggc ggt gtg cga ccg ttg act caa tgg gtc aat 1171
Glu Asp Asp Thr Lys Gly Gly Val Arg Pro Leu Thr Gln Trp Val Asn
345 350 355
ggt gaa gct att gcc tgg cgc act atc acc gag aat gac cag atg cga 1219
Gly Glu Ala Ile Ala Trp Arg Thr Ile Thr Glu Asn Asp Gln Met Arg
360 365 370
ctg ccc aat att ggt cag ttt atg acg gct aaa gaa tac cgt gcc gca 1267
Leu Pro Asn Ile Gly Gln Phe Met Thr Ala Lys Glu Tyr Arg Ala Ala
375 380 385
gcg tca tgg ctg cgt gct gga ctc att gat acc gca gat gtt gcg agt 1315
Ala Ser Trp Leu Arg Ala Gly Leu Ile Asp Thr Ala Asp Val Ala Ser
390 395 400 405
cag aaa att gac ccc aac aag gtc atg tct gat gag ggt atc gcg cgc 1363
Gln Lys Ile Asp Pro Asn Lys Val Met Ser Asp Glu Gly Ile Ala Arg
410 415 420
tcg gaa gct att ttg acg tat ctg tcg gat gag ggt gtg gat ttt agg 1411
Set Glu Ala Ile Leu Thr Tyr Leu Ser Asp Glu Gly Val Asp Phe Arg
425 430 435
atc gag cca gat cgt gag ccg gga cag ctt aaa gtg cgt att gag ggc 1459
Ile Glu Pro Asp Arg Glu Pro Gly Gln Leu Lys Val Arg Ile Glu Gly
440 445 450
acc cgt att gat att cgt ctc act gat act cgc gcc aat gaa cag tgg 1507
Thr Arg Ile Asp Ile Arg Leu Thr Asp Thr Arg Ala Asn Glu Gln Trp
455 460 465
gtg ggt cgt gtc tat gat tct ggc act gtg att aag tat tcc gcc gaa 1555
Val Gly Arg Val Tyr Asp Ser Gly Thr Val Ile Lys Tyr Ser Ala Glu
470 475 480 485
caa aca gcg gaa gaa cgt cta cga gct cgt gag cgc atg gag aac ggt 1603
Gln Thr Ala Glu Glu Arg Leu Arg Ala Arg Glu Arg Met Glu Asn Gly
490 495 500
gat gga acg tgg aca ccg gct act gat tat gaa cca agc cca act gaa 1651
Asp Gly Thr Trp Thr Pro Ala Thr Asp Tyr Glu Pro Ser Pro Thr Glu
505 510 515
gtt gtc gat ctc gtg aaa ttt gct ctc ggt cga gag gtt gag cga cag 1699
Val Val Asp Leu Val Lys Phe Ala Leu Gly Arg Glu Val Glu Arg Gln
520 525 530
gat ggc aaa ggt ctt gtt ggt gtg cct aat gct cga cac cca cgt gct 1747
Asp Gly Lys Gly Leu Val Gly Val Pro Asn Ala Arg His Pro Arg Ala
535 540 545
tta gag cag gca cag gat gca tat ttc act aag aat cgt tcc gca ttc 1795
Leu Glu Gln Ala Gln Asp Ala Tyr Phe Thr Lys Asn Arg Ser Ala Phe
550 555 560 565
atg gtg cgt gaa ggg ctc tcg atc gtt cag gat gca cgt gat cga agt 1843
Met Val Arg Glu Gly Leu Ser Ile Val Gln Asp Ala Arg Asp Arg Ser
570 575 580
gct ggt cct gga aag tgg ttt gat aat gaa gcg aaa gca tcg gag tgg 1891
Ala Gly Pro Gly Lys Trp Phe Asp Asn Glu Ala Lys Ala Ser Glu Trp
585 590 595
ctt ggt aac aat atc gcc ctt act cgt gcg cgt gtg gcc gaa gag ctc 1939
Leu Gly Asn Asn Ile Ala Leu Thr Arg Ala Arg Val Ala Glu Glu Leu
600 605 610
ggc gtt gaa gaa ctg att gcg ctt tct gca cag tac gcc gat gat cct 1987
Gly Val Glu Glu Leu Ile Ala Leu Ser Ala Gln Tyr Ala Asp Asp Pro
615 620 625
gac ttc atg ccg gct ttt gct ggt gag gat gag ctc atg gcg ata aag 2035
Asp Phe Met Pro Ala Phe Ala Gly Glu Asp Glu Leu Met Ala Ile Lys
630 635 640 645
cag gac tat tgg gcg atg ctg cga ggt gag gaa acc gat ctg ctt aac 2083
Gln Asp Tyr Trp Ala Met Leu Arg Gly Glu Glu Thr Asp Leu Leu Asn
650 655 660
cct ggg gtt aac cgc gat gat tat atg gct gct atc cgc gat ggt gat 2131
Pro Gly Val Asn Arg Asp Asp Tyr Met Ala Ala Ile Arg Asp Gly Asp
665 670 675
cat gag cag att gct gcg atg acc tca gca atg aat gct gtg acc gtg 2179
His Glu Gln Ile Ala Ala Met Thr Ser Ala Met Asn Ala Val Thr Val
680 685 690
gag gat cgt gtg cgt cag cac gcg gcc ctt gtt ctt gat gat tat gtc 2227
Glu Asp Arg Val Arg Gln His Ala Ala Leu Val Leu Asp Asp Tyr Val
695 700 705
ggc acg gtg gag cct gat cct gtt acc ggc ttg cgt ttt aat ccg gtg 2275
Gly Thr Val Glu Pro Asp Pro Val Thr Gly Leu Arg Phe Asn Pro Val
710 715 720 725
aca gtt gct cag cac atg ccg agt gct aaa tcc ctg tgg tca aac cat 2323
Thr Val Ala Gln His Met Pro Ser Ala Lys Ser Leu Trp Ser Asn His
730 735 740
gac gat att att gcc gcg ttg cga gcg act tcg att act ggc gat gaa 2371
Asp Asp Ile Ile Ala Ala Leu Arg Ala Thr Ser Ile Thr Gly Asp Glu
745 750 755
ctg cgc ggt gac gaa ttc tat aac gat gtt att aat caa cag ttg ctc 2419
Leu Arg Gly Asp Glu Phe Tyr Asn Asp Val Ile Asn Gln Gln Leu Leu
760 765 770
aaa ttt aat cct gag acc gcg cag aaa atg gtt aat aac cct gat ctt 2467
Lys Phe Asn Pro Glu Thr Ala Gln Lys Met Val Asn Asn Pro Asp Leu
775 780 785
gac ccg cag ttg gct cga ttt ggc act gtc att gcc gag act att tcc 2515
Asp Pro Gln Leu Ala Arg Phe Gly Thr Val Ile Ala Glu Thr Ile Ser
790 795 800 805
cgc aat ggt gct gat gtg gtc gat att gct gtt gac gat aac ggt gtg 2563
Arg Asn Gly Ala Asp Val Val Asp Ile Ala Val Asp Asp Asn Gly Val
810 815 820
gtg cgg tgg acc gca cag cga cgt gtt ggt gcg aag gat agt cgt gca 2611
Val Arg Trp Thr Ala Gln Arg Arg Val Gly Ala Lys Asp Ser Arg Ala
825 830 835
gta gat agt aag ggg cag gtt cgc ggt gaa cgc acc cgt cat gtg cag 2659
Val Asp Ser Lys Gly Gln Val Arg Gly Glu Arg Thr Arg His Val Gln
840 845 850
ggt gag ata ggt cag att ttt act cgc ggt gag cat ggt gaa att gtc 2707
Gly Glu Ile Gly Gln Ile Phe Thr Arg Gly Glu His Gly Glu Ile Val
855 860 865
acc aaa ttt aat ggt ggc gag aac tat atg ttt gcc cct ggc tac acc 2755
Thr Lys Phe Asn Gly Gly Glu Asn Tyr Met Phe Ala Pro Gly Tyr Thr
870 875 880 885
gcg tct gtg gta cca caa aag cct ggt gag aca aag agt tta gaa gag 2803
Ala Ser Val Val Pro Gln Lys Pro Gly Glu Thr Lys Ser Leu Glu Glu
890 895 900
cgt acc aag ctc aaa ggc tat gag cag gtg atg tca gag gcg ttg gtg 2851
Arg Thr Lys Leu Lys Gly Tyr Glu Gln Val Met Ser Glu Ala Leu Val
905 910 915
tac cgt gtg cga gaa gat ttg atg ttc act gag cga tcc cga gtg ggt 2899
Tyr Arg Val Arg Glu Asp Leu Met Phe Thr Glu Arg Ser Arg Val Gly
920 925 930
gct aca acc agc atc aac agt gca tat aag cga ctg tat gac aat cgc 2947
Ala Thr Thr Ser Ile Asn Ser Ala Tyr Lys Arg Leu Tyr Asp Asn Arg
935 940 945
ttc cct gtg gac ttc ttc gag cgc agc gcc gaa gaa ggt ttg agc gat 2995
Phe Pro Val Asp Phe Phe Glu Arg Ser Ala Glu Glu Gly Leu Ser Asp
950 955 960 965
gag tgg cgt gct gcg ttg ctt gag act gcg agt ttg cgt gtg cgc tac 3043
Glu Trp Arg Ala Ala Leu Leu Glu Thr Ala Ser Leu Arg Val Arg Tyr
970 975 980
gac aac tct att cgt gat ggt gcg aat gtg atg gag gat att cga gca 3091
Asp Asn Ser Ile Arg Asp Gly Ala Asn Val Met Glu Asp Ile Arg Ala
985 990 995
cag cag cgt ggt ttt gat gcg cgt aat gat aac tcc cgt gat gcg ctg 3139
Gln Gln Arg Gly Phe Asp Ala Arg Asn Asp Asn Ser Arg Asp Ala Leu
1000 1005 1010
gtg ctt acc ggt ggg cgt aac att tct gta ctt gat gtt gat gcg ggt 3187
Val Leu Thr Gly Gly Arg Asn Ile Ser Val Leu Asp Val Asp Ala Gly
1015 1020 1025
aaa ggt ttc ttt gat ccc atg atg act ggt atg gcg gcg aac cag ggt 3235
Lys Gly Phe Phe Asp Pro Met Met Thr Gly Met Ala Ala Asn Gln Gly
1030 1035 1040 1045
tct gtt cgc tat ttg ttg cct tct gcg aag gtg ggc gct gat ggc atg 3283
Ser Val Arg Tyr Leu Leu Pro Ser Ala Lys Val Gly Ala Asp Gly Met
1050 1055 1060
att acc cct ggt gat ccg gct gat cga gta cct gtt gca gcg cat cca 3331
Ile Thr Pro Gly Asp Pro Ala Asp Arg Val Pro Val Ala Ala His Pro
1065 1070 1075
gaa tca tgg gcg atg ggc ttt gac cca cat gac cgt cag aac atg act 3379
Glu Ser Trp Ala Met Gly Phe Asp Pro His Asp Arg Gln Asn Met Thr
1080 1085 1090
ttc tcg aac atc atg cag gct agc gct gtg act ggt ggt gca cgc act 3427
Phe Ser Asn Ile Met Gln Ala Ser Ala Val Thr Gly Gly Ala Arg Thr
1095 1100 1105
gcg atg ata cag ctg ggc ggt tgg aac ttt gaa gat ggc att att gtc 3475
Ala Met Ile Gln Leu Gly Gly Trp Asn Phe Glu Asp Gly Ile Ile Val
1110 1115 1120 1125
tct gcc gat ttt gcg aat acc cat gtt att cgc gat acg gaa gat gag 3523
Ser Ala Asp Phe Ala Asn Thr His Val Ile Arg Asp Thr Glu Asp Glu
1130 1135 1140
atg cgc ccc ttg gtt gcc ggc gat aag ctg tct gat ttt cac ggt aat 3571
Met Arg Pro Leu Val Ala Gly Asp Lys Leu Ser Asp Phe His Gly Asn
1145 1150 1155
aag ggt gtg atc gct ctt gtt gtt gat cca gcg atg agt gat gct gat 3619
Lys Gly Val Ile Ala Leu Val Val Asp Pro Ala Met Ser Asp Ala Asp
1160 1165 1170
gca cgt gca gca ggg ctg gaa tca gag gtg gcg ttt ttc cga gac aac 3667
Ala Arg Ala Ala Gly Leu Glu Ser Glu Val Ala Phe Phe Arg Asp Asn
1175 1180 1185
cct gat cta gag gtt gtc atg agt ccg ttt tct gcc att tct cgt ttt 3715
Pro Asp Leu Glu Val Val Met Ser Pro Phe Ser Ala Ile Ser Arg Phe
1190 1195 1200 1205
aat ggt ggc acg gca cgt gag ctc atg aac aac ccg cag gat gtg gtg 3763
Asn Gly Gly Thr Ala Arg Glu Leu Met Asn Asn Pro Gln Asp Val Val
1210 1215 1220
ttc agg aat aat gat ggt tct act cgt gtt caa cca ggt gcc tct ggt 3811
Phe Arg Asn Asn Asp Gly Ser Thr Arg Val Gln Pro Gly Ala Ser Gly
1225 1230 1235
gat ctc aac ttc atc gtc acg cat atg gct gtt gat gcg aaa acc aat 3859
Asp Leu Asn Phe Ile Val Thr His Met Ala Val Asp Ala Lys Thr Asn
1240 1245 1250
gtc tat gat gaa gaa gct gtt cgt gag ggc caa gga cgt aaa gcg tct 3907
Val Tyr Asp Glu Glu Ala Val Arg Glu Gly Gln Gly Arg Lys Ala Ser
1255 1260 1265
tct cag ctg gcg tgg gtg ctc caa gcc cag gaa tgt cat gag att atg 3955
Ser Gln Leu Ala Trp Val Leu Gln Ala Gln Glu Cys His Glu Ile Met
1270 1275 1280 1285
gag cac ttt tat ggc aac aac gtc tct gca ttg gct aac ttc cag gaa 4003
Glu His Phe Tyr Gly Asn Asn Val Ser Ala Leu Ala Asn Phe Gln Glu
1290 1295 1300
tat ctt cgc gtc acg ggg ctt gat gtc acc ccg tat ggt gag ttg cgc 4051
Tyr Leu Arg Val Thr Gly Leu Asp Val Thr Pro Tyr Gly Glu Leu Arg
1305 1310 1315
gag ggg ttc agc gag tcc aat gag cag cgc aat atc atc gag atg cca 4099
Glu Gly Phe Ser Glu Ser Asn Glu Gln Arg Asn Ile Ile Glu Met Pro
1320 1325 1330
agc att tac gat gag aac ggt gag ctg aat aat cgt gtt aat cgt gca 4147
Ser Ile Tyr Asp Glu Asn Gly Glu Leu Asn Asn Arg Val Asn Arg Ala
1335 1340 1345
cag gtg cgc gaa gct ttt gtt gag cag atc agt cgc gct ggt ggt gtt 4195
Gln Val Arg Glu Ala Phe Val Glu Gln Ile Ser Arg Ala Gly Gly Val
1350 1355 1360 1365
atg gag att cct ttc cag tta cag ttg cgc aat ggt gca cag cta gaa 4243
Met Glu Ile Pro Phe Gln Leu Gln Leu Arg Asn Gly Ala Gln Leu Glu
1370 1375 1380
gag tca cct aat aat cca gac atg tat cag ttg cca cta ctg agc ccg 4291
Glu Ser Pro Asn Asn Pro Asp Met Tyr Gln Leu Pro Leu Leu Ser Pro
1385 1390 1395
cat ttg cgc agt gat gag gat tta gcg gac ggt agt acc tct cga cat 4339
His Leu Arg Ser Asp Glu Asp Leu Ala Asp Gly Ser Thr Ser Arg His
1400 1405 1410
gag tac acc acg cgg tat atg gct att ttt gat acg tgc tac aag ctg 4387
Glu Tyr Thr Thr Arg Tyr Met Ala Ile Phe Asp Thr Cys Tyr Lys Leu
1415 1420 1425
gca gaa gag caa gcc aag att gac gca ttg cgc cag gag gaa gcg cag 4435
Ala Glu Glu Gln Ala Lys Ile Asp Ala Leu Arg Gln Glu Glu Ala Gln
1430 1435 1440 1445
cac ggc acg ttg cct cgt gct gcg gaa aag cga ctg agc gag tca cag 4483
His Gly Thr Leu Pro Arg Ala Ala Glu Lys Arg Leu Ser Glu Ser Gln
1450 1455 1460
aag ttt gtt gat caa gca cag aaa aag gtg cag gca agc ttt gat gga 4531
Lys Phe Val Asp Gln Ala Gln Lys Lys Val Gln Ala Ser Phe Asp Gly
1465 1470 1475
att gct aat gat att gtc gcc agc cgg atc gag acc aag aac aat gtg 4579
Ile Ala Asn Asp Ile Val Ala Ser Arg Ile Glu Thr Lys Asn Asn Val
1480 1485 1490
ttc aag gac ggc ttt atg tcg gcg cga cag tca cac tcc gct act gct 4627
Phe Lys Asp Gly Phe Met Ser Ala Arg Gln Ser His Ser Ala Thr Ala
1495 1500 1505
gtg tgg aca ggt gat cca cgc ctg tca gtt gat gaa gta gcc atg aac 4675
Val Trp Thr Gly Asp Pro Arg Leu Ser Val Asp Glu Val Ala Met Asn
1510 1515 1520 1525
tct tcg atg gca cgt gaa ctc ggc gtt ctc gat aac ggt tat gca atg 4723
Ser Ser Met Ala Arg Glu Leu Gly Val Leu Asp Asn Gly Tyr Ala Met
1530 1535 1540
gtg tgg cgt gat ccg gtg atc cgc gat ggt ggt gtg cgc tat ttg cgt 4771
Val Trp Arg Asp Pro Val Ile Arg Asp Gly Gly Val Arg Tyr Leu Arg
1545 1550 1555
gtg gtt att aat gat gat ctg cac ggt gtg gca gtc aac cct gtg tcg 4819
Val Val Ile Asn Asp Asp Leu His Gly Val Ala Val Asn Pro Val Ser
1560 1565 1570
gtg aag agc ttt gac ggt gac ttc gat ggt gac tct gta ggt ctg gtc 4867
Val Lys Ser Phe Asp Gly Asp Phe Asp Gly Asp Ser Val Gly Leu Val
1575 1580 1585
ggc aat ctg ccg aaa aag gcg cat gag gag gca ttg tcc agg ctc aca 4915
Gly Asn Leu Pro Lys Lys Ala His Glu Glu Ala Leu Ser Arg Leu Thr
1590 1595 1600 1605
gtt gag gca aac atg ctt gat ctt ggt gat ggt aag cgt atg gag gac 4963
Val Glu Ala Asn Met Leu Asp Leu Gly Asp Gly Lys Arg Met Glu Asp
1610 1615 1620
ggc act atg ttt tat ggg cta act ctg cat gac agc ctc gat gta cag 5011
Gly Thr Met Phe Tyr Gly Leu Thr Leu His Asp Ser Leu Asp Val Gln
1625 1630 1635
gta gcg cag cac cat gat ccg gca atg gcc gag aac atg aag gtt att 5059
Val Ala Gln His His Asp Pro Ala Met Ala Glu Asn Met Lys Val Ile
1640 1645 1650
gtc tct gag ctt aac cgc tac cag cgt gaa tac gag gcc ggc gag att 5107
Val Ser Glu Leu Asn Arg Tyr Gln Arg Glu Tyr Glu Ala Gly Glu Ile
1655 1660 1665
agc cgc gaa gaa ttg cta gag gtc aac cgt gca cac atg gat gat ctc 5155
Ser Arg Glu Glu Leu Leu Glu Val Asn Arg Ala His Met Asp Asp Leu
1670 1675 1680 1685
aat gag cac tat gca cag gct ttt gcc aac cgc gat ggt ttg gtg acc 5203
Asn Glu His Tyr Ala Gln Ala Phe Ala Asn Arg Asp Gly Leu Val Thr
1690 1695 1700
ttg cgt ttt gac ggt atg gaa aac cat atg gct tct gtt gcg cag tgc 5251
Leu Arg Phe Asp Gly Met Glu Asn His Met Ala Ser Val Ala Gln Cys
1705 1710 1715
ttc gag acg ggt gcg aaa ggt tcg cct ggc aag ctg aag gaa tat gcc 5299
Phe Glu Thr Gly Ala Lys Gly Ser Pro Gly Lys Leu Lys Glu Tyr Ala
1720 1725 1730
acc tat atc ggc gct gat ccg gcg cag ggt ttt aaa gac gta ggt caa 5347
Thr Tyr Ile Gly Ala Asp Pro Ala Gln Gly Phe Lys Asp Val Gly Gln
1735 1740 1745
cca acg cca gaa gcg ctg cgt agc cac tat gag ggt tcg cag aag gcg 5395
Pro Thr Pro Glu Ala Leu Arg Ser His Tyr Glu Gly Ser Gln Lys Ala
1750 1755 1760 1765
act gca att aag gtg ctc ttt acc ggt gtg gct ggt aaa aaa gag cag 5443
Thr Ala Ile Lys Val Leu Phe Thr Gly Val Ala Gly Lys Lys Glu Gln
1770 1775 1780
gaa atg gtg gca ttg tgc cgc aat ctt ggt ctg acc aag gaa gct atg 5491
Glu Met Val Ala Leu Cys Arg Asn Leu Gly Leu Thr Lys Glu Ala Met
1785 1790 1795
gct gca tca gca ccg gca cag cag tcg att ctg cag gcg aag cac gac 5539
Ala Ala Ser Ala Pro Ala Gln Gln Ser Ile Leu Gln Ala Lys His Asp
1800 1805 1810
ccg att gac gcg ctc tat cgt gca gaa act ctt atg gga cca gta ggt 5587
Pro Ile Asp Ala Leu Tyr Arg Ala Glu Thr Leu Met Gly Pro Val Gly
1815 1820 1825
gat ctc tac caa ggt cgc aag atg cgt cgt ggt gag aat gag caa ggt 5635
Asp Leu Tyr Gln Gly Arg Lys Met Arg Arg Gly Glu Asn Glu Gln Gly
1830 1835 1840 1845
cgc tat gag tgg gaa gtc gtt agg gac gag aac cac cag cct atc cag 5683
Arg Tyr Glu Trp Glu Val Val Arg Asp Glu Asn His Gln Pro Ile Gln
1850 1855 1860
gcc acc aag gat gaa tgg gta cag cag tac atg gag atg tac gcc gat 5731
Ala Thr Lys Asp Glu Trp Val Gln Gln Tyr Met Glu Met Tyr Ala Asp
1865 1870 1875
gac aag ggt atg ggt gtc tcg gtt ggc gtt gac cag gtg gag aag atc 5779
Asp Lys Gly Met Gly Val Ser Val Gly Val Asp Gln Val Glu Lys Ile
1880 1885 1890
gcg gaa gaa ttc tct gat gag cag ggt tat atg cgt gtg ctt agc cat 5827
Ala Glu Glu Phe Ser Asp Glu Gln Gly Tyr Met Arg Val Leu Ser His
1895 1900 1905
gat gag cta ccc act gag att aag cca cta gca ctt gat cag ctt gcc 5875
Asp Glu Leu Pro Thr Glu Ile Lys Pro Leu Ala Leu Asp Gln Leu Ala
1910 1915 1920 1925
tat ggc gat aag aag aac cgc ttt gat ttg ttg tgc gag atg gca aag 5923
Tyr Gly Asp Lys Lys Asn Arg Phe Asp Leu Leu Cys Glu Met Ala Lys
1930 1935 1940
cag cag gtc aac atc tac gac ggt gat gct tat gac ttt gcc cct cgt 5971
Gln Gln Val Asn Ile Tyr Asp Gly Asp Ala Tyr Asp Phe Ala Pro Arg
1945 1950 1955
gtg gtg cgc gcc aat atg aag gca atg gag gat gca gcg cgt ttt ggt 6019
Val Val Arg Ala Asn Met Lys Ala Met Glu Asp Ala Ala Arg Phe Gly
1960 1965 1970
atc cct gat gtg gag att cag tcg att tct gca cag cag tca ttg gcg 6067
Ile Pro Asp Val Glu Ile Gln Ser Ile Ser Ala Gln Gln Ser Leu Ala
1975 1980 1985
agc ttt gag cga gtc cca caa cgt tct ggt ttc cgt att gag cgt cgt 6115
Ser Phe Glu Arg Val Pro Gln Arg Ser Gly Phe Arg Ile Glu Arg Arg
1990 1995 2000 2005
gtg ccg gca gaa gta ggt aca ggg att gct gca cca gca ccg tta cct 6163
Val Pro Ala Glu Val Gly Thr Gly Ile Ala Ala Pro Ala Pro Leu Pro
2010 2015 2020
gat gca ggt gta cag ggg aat tat gtt cag cag cag gtg cct gcg acc 6211
Asp Ala Gly Val Gln Gly Asn Tyr Val Gln Gln Gln Val Pro Ala Thr
2025 2030 2035
ccg cca gtt atg cct gtc aca cct ccg gca cag caa cct gtt tcg cct 6259
Pro Pro Val Met Pro Val Thr Pro Pro Ala Gln Gln Pro Val Ser Pro
2040 2045 2050
gct cag acg gac ttc cgg ggc gtt ggt cag cca ctc gac cag ggc gga 6307
Ala Gln Thr Asp Phe Arg Gly Val Gly Gln Pro Leu Asp Gln Gly Gly
2055 2060 2065
aaa atc ggg aat tat gga cat cag gga cct gca atg ggg gcg caa cag 6355
Lys Ile Gly Asn Tyr Gly His Gln Gly Pro Ala Met Gly Ala Gln Gln
2070 2075 2080 2085
cca gtt gtg cct cag cag cag aat att ccg cct gtg cat aat ccg gta 6403
Pro Val Val Pro Gln Gln Gln Asn Ile Pro Pro Val His Asn Pro Val
2090 2095 2100
cca cag aat tct gtt ccg cct act ccg gtt gtg ccg aag cca ggt aca 6451
Pro Gln Asn Ser Val Pro Pro Thr Pro Val Val Pro Lys Pro Gly Thr
2105 2110 2115
gga aac ccg ttt act cat ggt ggt gcg aac aat cag ttt atg ggt cga 6499
Gly Asn Pro Phe Thr His Gly Gly Ala Asn Asn Gln Phe Met Gly Arg
2120 2125 2130
ttt gat aca agc cgt tac aac caa cag gaa cca cca caa cga cag gat 6547
Phe Asp Thr Ser Arg Tyr Asn Gln Gln Glu Pro Pro Gln Arg Gln Asp
2135 2140 2145
ggc ggg ttt gag ctc taaataatgg cagtaga tttcagcagaaaa 6592
Gly Gly Phe Glu Leu
2150
<210>172
<211>2154
<212>PRT
<213>谷氨酸棒状杆菌
<400>172
Met Ala Arg Ser Glu Trp Gly Gln Lys Asp His Trp Gly Lys Ala Pro
1 5 10 15
Leu Gly Asp His Asp Ala Val Arg Ile Tyr Arg Gln Ala Glu Ala Leu
20 25 30
Met Lys Gln Arg Ala Ala Asn Asn Lys Gly Leu Asp Leu Asn Asp Glu
35 40 45
Ala Thr Tyr Gly Ala Ile Met Phe Ala Ser Phe Asp Asp Ala Phe Ala
50 55 60
Ala Asp Ala Glu Ala His Gly Cys Val His Arg Pro Gln Phe Ala Lys
65 70 75 80
Val Thr Arg Arg Asn Ile Leu Asn Ser Pro Gln Tyr Thr Ala Ile Lys
85 90 95
Ser Ser Tyr Asp Ala Leu Val Gln Gln Asp Leu Val Ala Gln Gln Gln
100 105 110
Gln Ala Gln Ala Leu Gln Gln Ala Ala Tyr Glu Gln Glu Gln Ala Ala
115 120 125
Ile Phe Gln Ala Arg Asp Gln Ala Thr Phe Gln Ala Glu Gln Thr Leu
130 135 140
Ala Gln Glu Ser Val Arg Gln Ser Glu Gly Val Gly Leu Pro Val Ile
145 150 155 160
Asp Pro Met Glu Ser Tyr Phe Arg Glu Tyr Ala Phe Tyr Glu Gly Val
165 170 175
Glu Met Phe Gly Thr Trp Gly Thr His Val Asp Ala Gly Val Ser Gln
180 185 190
Phe Ser Thr Ser Asp Gly Arg Ala Ile Arg Ala Leu Val Asp Glu His
195 200 205
Leu Ala Thr Tyr Glu Pro Val Glu Gln Arg Gly Ser Thr Gln Ala Phe
210 215 220
Phe Ala Ser Val Asn Ala Ala Phe Ala Glu Val Ala Pro His Ala Ala
225 230 235 240
Pro Met Phe Ser Ala Ile Ala Arg Glu Gly Glu Gly Ser Lys Gly Tyr
245 250 255
Gln Ala Leu Arg His Arg Phe Asp Lys Met Val Gly Thr Ala Ile Gly
260 265 270
Pro Asn Gly Leu Pro Glu Gly Pro Asp Leu Gly Gly Arg Arg Leu Pro
275 280 285
Ile Ser Pro Tyr Asp Pro Arg Trp Ser Asp Arg Glu Val Val Arg Val
290 295 300
His Gly Thr Lys Leu Leu Ser Leu Ser Glu Glu Ser Val Gly Met Ile
305 310 315 320
Arg Asp Leu Asp Leu Ala Thr Ile His Leu Gln Asp Asn Asp Val Tyr
325 330 335
Glu Gly Thr His Arg Glu Asp Asp Thr Lys Gly Gly Val Arg Pro Leu
340 345 350
Thr Gln Trp Val Asn Gly Glu Ala Ile Ala Trp Arg Thr Ile Thr Glu
355 360 365
Asn Asp Gln Met Arg Leu Pro Asn Ile Gly Gln Phe Met Thr Ala Lys
370 375 380
Glu Tyr Arg Ala Ala Ala Ser Trp Leu Arg Ala Gly Leu Ile Asp Thr
385 390 395 400
Ala Asp Val Ala Ser Gln Lys Ile Asp Pro Asn Lys Val Met Ser Asp
405 410 415
Glu Gly Ile Ala Arg Ser Glu Ala Ile Leu Thr Tyr Leu Ser Asp Glu
420 425 430
Gly Val Asp Phe Arg Ile Glu Pro Asp Arg Glu Pro Gly Gln Leu Lys
435 440 445
Val Arg Ile Glu Gly Thr Arg Ile Asp Ile Arg Leu Thr Asp Thr Arg
450 455 460
Ala Asn Glu Gln Trp Val Gly Arg Val Tyr Asp Ser Gly Thr Val Ile
465 470 475 480
Lys Tyr Ser Ala Glu Gln Thr Ala Glu Glu Arg Leu Arg Ala Arg Glu
485 490 495
Arg Met Glu Asn Gly Asp Gly Thr Trp Thr Pro Ala Thr Asp Tyr Glu
500 505 510
Pro Ser Pro Thr Glu Val Val Asp Leu Val Lys Phe Ala Leu Gly Arg
515 520 525
Glu Val Glu Arg Gln Asp Gly Lys Gly Leu Val Gly Val Pro Asn Ala
530 535 540
Arg His Pro Arg Ala Leu Glu Gln Ala Gln Asp Ala Tyr Phe Thr Lys
545 550 555 560
Asn Arg Ser Ala Phe Met Val Arg Glu Gly Leu Ser Ile Val Gln Asp
565 570 575
Ala Arg Asp Arg Ser Ala Gly Pro Gly Lys Trp Phe Asp Asn Glu Ala
580 585 590
Lys Ala Ser Glu Trp Leu Gly Asn Asn Ile Ala Leu Thr Arg Ala Arg
595 600 605
Val Ala Glu Glu Leu Gly Val Glu Glu Leu Ile Ala Leu Ser Ala Gln
610 615 620
Tyr Ala Asp Asp Pro Asp Phe Met Pro Ala Phe Ala Gly Glu Asp Glu
625 630 635 640
Leu Met Ala Ile Lys Gln Asp Tyr Trp Ala Met Leu Arg Gly Glu Glu
645 650 655
Thr Asp Leu Leu Asn Pro Gly Val Asn Arg Asp Asp Tyr Met Ala Ala
660 665 670
Ile Arg Asp Gly Asp His Glu Gln Ile Ala Ala Met Thr Ser Ala Met
675 680 685
Asn Ala Val Thr Val Glu Asp Arg Val Arg Gln His Ala Ala Leu Val
690 695 700
Leu Asp Asp Tyr Val Gly Thr Val Glu Pro Asp Pro Val Thr Gly Leu
705 710 715 720
Arg Phe Asn Pro Val Thr Val Ala Gln His Met Pro Ser Ala Lys Ser
725 730 735
Leu Trp Ser Asn His Asp Asp Ile Ile Ala Ala Leu Arg Ala Thr Ser
740 745 750
Ile Thr Gly Asp Glu Leu Arg Gly Asp Glu Phe Tyr Asn Asp Val Ile
755 760 765
Asn Gln Gln Leu Leu Lys Phe Asn Pro Glu Thr Ala Gln Lys Met Val
770 775 780
Asn Asn Pro Asp Leu Asp Pro Gln Leu Ala Arg Phe Gly Thr Val Ile
785 790 795 800
Ala Glu Thr Ile Ser Arg Asn Gly Ala Asp Val Val Asp Ile Ala Val
805 810 815
Asp Asp Asn Gly Val Val Arg Trp Thr Ala Gln Arg Arg Val Gly Ala
820 825 830
Lys Asp Ser Arg Ala Val Asp Ser Lys Gly Gln Val Arg Gly Glu Arg
835 840 845
Thr Arg His Val Gln Gly Glu Ile Gly Gln Ile Phe Thr Arg Gly Glu
850 855 860
His Gly Glu Ile Val Thr Lys Phe Asn Gly Gly Glu Asn Tyr Met Phe
865 870 875 880
Ala Pro Gly Tyr Thr Ala Ser Val Val Pro Gln Lys Pro Gly Glu Thr
885 890 895
Lys Ser Leu Glu Glu Arg Thr Lys Leu Lys Gly Tyr Glu Gln Val Met
900 905 910
Ser Glu Ala Leu Val Tyr Arg Val Arg Glu Asp Leu Met Phe Thr Glu
915 920 925
Arg Ser Arg Val Gly Ala Thr Thr Ser Ile Asn Ser Ala Tyr Lys Arg
930 935 940
Leu Tyr Asp Asn Arg Phe Pro Val Asp Phe Phe Glu Arg Ser Ala Glu
945 950 955 960
Glu Gly Leu Ser Asp Glu Trp Arg Ala Ala Leu Leu Glu Thr Ala Ser
965 970 975
Leu Arg Val Arg Tyr Asp Asn Ser Ile Arg Asp Gly Ala Asn Val Met
980 985 990
Glu Asp Ile Arg Ala Gln Gln Arg Gly Phe Asp Ala Arg Asn Asp Asn
995 1000 1005
Ser Arg Asp Ala Leu Val Leu Thr Gly Gly Arg Asn Ile Ser Val Leu
1010 1015 1020
Asp Val Asp Ala Gly Lys Gly Phe Phe Asp Pro Met Met Thr Gly Met
1025 1030 1035 1040
Ala Ala Asn Gln Gly Ser Val Arg Tyr Leu Leu Pro Ser Ala Lys Val
1045 1050 1055
Gly Ala Asp Gly Met Ile Thr Pro Gly Asp Pro Ala Asp Arg Val Pro
1060 1065 1070
Val Ala Ala His Pro Glu Ser Trp Ala Met Gly Phe Asp Pro His Asp
1075 1080 1085
Arg Gln Asn Met Thr Phe Ser Asn Ile Met Gln Ala Ser Ala Val Thr
1090 1095 1100
Gly Gly Ala Arg Thr Ala Met Ile Gln Leu Gly Gly Trp Asn Phe Glu
1105 1110 1115 1120
Asp Gly Ile Ile Val Ser Ala Asp Phe Ala Asn Thr His Val Ile Arg
1125 1130 1135
Asp Thr Glu Asp Glu Met Arg Pro Leu Val Ala Gly Asp Lys Leu Ser
1140 1145 1150
Asp Phe His Gly Asn Lys Gly Val Ile Ala Leu Val Val Asp Pro Ala
1155 1160 1165
Met Ser Asp Ala Asp Ala Arg Ala Ala Gly Leu Glu Ser Glu Val Ala
1170 1175 1180
Phe Phe Arg Asp Asn Pro Asp Leu Glu Val Val Met Ser Pro Phe Ser
1185 1190 1195 1200
Ala Ile Ser Arg Phe Asn Gly Gly Thr Ala Arg Glu Leu Met Asn Asn
1205 1210 1215
Pro Gln Asp Val Val Phe Arg Asn Asn Asp Gly Ser Thr Arg Val Gln
1220 1225 1230
Pro Gly Ala Ser Gly Asp Leu Asn Phe Ile Val Thr His Met Ala Val
1235 1240 1245
Asp Ala Lys Thr Asn Val Tyr Asp Glu Glu Ala Val Arg Glu Gly Gln
1250 1255 1260
Gly Arg Lys Ala Ser Ser Gln Leu Ala Trp Val Leu Gln Ala Gln Glu
1265 1270 1275 1280
Cys His Glu Ile Met Glu His Phe Tyr Gly Asn Asn Val Ser Ala Leu
1285 1290 1295
Ala Asn Phe Gln Glu Tyr Leu Arg Val Thr Gly Leu Asp Val Thr Pro
1300 1305 1310
Tyr Gly Glu Leu Arg Glu Gly Phe Ser Glu Ser Asn Glu Gln Arg Asn
1315 1320 1325
Ile Ile Glu Met Pro Ser Ile Tyr Asp Glu Asn Gly Glu Leu Asn Asn
1330 1335 1340
Arg Val Asn Arg Ala Gln Val Arg Glu Ala Phe Val Glu Gln Ile Ser
1345 1350 1355 1360
Arg Ala Gly Gly Val Met Glu Ile Pro Phe Gln Leu Gln Leu Arg Asn
1365 1370 1375
Gly Ala Gln Leu Glu Glu Ser Pro Asn Asn Pro Asp Met Tyr Gln Leu
1380 1385 1390
Pro Leu Leu Ser Pro His Leu Arg Ser Asp Glu Asp Leu Ala Asp Gly
1395 1400 1405
Ser Thr Ser Arg His Glu Tyr Thr Thr Arg Tyr Met Ala Ile Phe Asp
1410 1415 1420
Thr Cys Tyr Lys Leu Ala Glu Glu Gln Ala Lys Ile Asp Ala Leu Arg
1425 1430 1435 1440
Gln Glu Glu Ala Gln His Gly Thr Leu Pro Arg Ala Ala Glu Lys Arg
1445 1450 1455
Leu Ser Glu Ser Gln Lys Phe Val Asp Gln Ala Gln Lys Lys Val Gln
1460 1465 1470
Ala Ser Phe Asp Gly Ile Ala Asn Asp Ile Val Ala Ser Arg Ile Glu
1475 1480 1485
Thr Lys Asn Asn Val Phe Lys Asp Gly Phe Met Ser Ala Arg Gln Ser
1490 1495 1500
His Ser Ala Thr Ala Val Trp Thr Gly Asp Pro Arg Leu Ser Val Asp
1505 1510 1515 1520
Glu Val Ala Met Asn Ser Ser Met Ala Arg Glu Leu Gly Val Leu Asp
1525 1530 1535
Asn Gly Tyr Ala Met Val Trp Arg Asp Pro Val Ile Arg Asp Gly Gly
1540 1545 1550
Val Arg Tyr Leu Arg Val Val Ile Asn Asp Asp Leu His Gly Val Ala
1555 1560 1565
Val Asn Pro Val Ser Val Lys Ser Phe Asp Gly Asp Phe Asp Gly Asp
1570 1575 1580
Ser Val Gly Leu Val Gly Asn Leu Pro Lys Lys Ala His Glu Glu Ala
1585 1590 1595 1600
Leu Ser Arg Leu Thr Val Glu Ala Asn Met Leu Asp Leu Gly Asp Gly
1605 1610 1615
Lys Arg Met Glu Asp Gly Thr Met Phe Tyr Gly Leu Thr Leu His Asp
1620 1625 1630
Ser Leu Asp Val Gln Val Ala Gln His His Asp Pro Ala Met Ala Glu
1635 1640 1645
Asn Met Lys Val Ile Val Ser Glu Leu Asn Arg Tyr Gln Arg Glu Tyr
1650 1655 1660
Glu Ala Gly Glu Ile Ser Arg Glu Glu Leu Leu Glu Val Asn Arg Ala
1665 1670 1675 1680
His Met Asp Asp Leu Asn Glu His Tyr Ala Gln Ala Phe Ala Asn Arg
1685 1690 1695
Asp Gly Leu Val Thr Leu Arg Phe Asp Gly Met Glu Asn His Met Ala
1700 1705 1710
Ser Val Ala Gln Cys Phe Glu Thr Gly Ala Lys Gly Ser Pro Gly Lys
1715 1720 1725
Leu Lys Glu Tyr Ala Thr Tyr Ile Gly Ala Asp Pro Ala Gln Gly Phe
1730 1735 1740
Lys Asp Val Gly Gln Pro Thr Pro Glu Ala Leu Arg Ser His Tyr Glu
1745 1750 1755 1760
Gly Ser Gln Lys Ala Thr Ala Ile Lys Val Leu Phe Thr Gly Val Ala
1765 1770 1775
Gly Lys Lys Glu Gln Glu Met Val Ala Leu Cys Arg Asn Leu Gly Leu
1780 1785 1790
Thr Lys Glu Ala Met Ala Ala Ser Ala Pro Ala Gln Gln Ser Ile Leu
1795 1800 1805
Gln Ala Lys His Asp Pro Ile Asp Ala Leu Tyr Arg Ala Glu Thr Leu
1810 1815 1820
Met Gly Pro Val Gly Asp Leu Tyr Gln Gly Arg Lys Met Arg Arg Gly
1825 1830 1835 1840
Glu Asn Glu Gln Gly Arg Tyr Glu Trp Glu Val Val Arg Asp Glu Asn
1845 1850 1855
His Gln Pro Ile Gln Ala Thr Lys Asp Glu Trp Val Gln Gln Tyr Met
1860 1865 1870
Glu Met Tyr Ala Asp Asp Lys Gly Met Gly Val Ser Val Gly Val Asp
1875 1880 1885
Gln Val Glu Lys Ile Ala Glu Glu Phe Ser Asp Glu Gln Gly Tyr Met
1890 1895 1900
Arg Val Leu Ser His Asp Glu Leu Pro Thr Glu Ile Lys Pro Leu Ala
1905 1910 1915 1920
Leu Asp Gln Leu Ala Tyr Gly Asp Lys Lys Asn Arg Phe Asp Leu Leu
1925 1930 1935
Cys Glu Met Ala Lys Gln Gln Val Asn Ile Tyr Asp Gly Asp Ala Tyr
1940 1945 1950
Asp Phe Ala Pro Arg Val Val Arg Ala Asn Met Lys Ala Met Glu Asp
1955 1960 1965
Ala Ala Arg Phe Gly Ile Pro Asp Val Glu Ile Gln Ser Ile Ser Ala
1970 1975 1980
Gln Gln Ser Leu Ala Ser Phe Glu Arg Val Pro Gln Arg Ser Gly Phe
1985 1990 1995 2000
Arg Ile Glu Arg Arg Val Pro Ala Glu Val Gly Thr GlyIle Ala Ala
2005 2010 2015
Pro Ala Pro Leu Pro Asp Ala Gly Val Gln Gly Asn Tyr Val Gln Gln
2020 2025 2030
Gln Val Pro Ala Thr Pro Pro Val Met Pro Val Thr Pro Pro Ala Gln
2035 2040 2045
Gln Pro Val Ser Pro Ala Gln Thr Asp Phe Arg Gly Val Gly Gln Pro
2050 2055 2060
Leu Asp Gln Gly Gly Lys Ile Gly Asn Tyr Gly His Gln Gly Pro Ala
2065 2070 2075 2080
Met Gly Ala Gln Gln Pro Val Val Pro Gln Gln Gln Asn Ile Pro Pro
2085 2090 2095
Val His Asn Pro Val Pro Gln Asn Ser Val Pro Pro Thr Pro Val Val
2100 2105 2110
Pro Lys Pro Gly Thr Gly Asn Pro Phe Thr His Gly Gly Ala Asn Asn
2115 2120 2125
Gln Phe Met Gly Arg Phe Asp Thr Ser Arg Tyr Asn Gln Gln Glu Pro
2130 2135 2140
Pro Gln Arg Gln Asp Gly Gly Phe Glu Leu
2145 2150
<210>173
<211>1216
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1186)
<223>RXA01628
<400>173
attaagaaaa aatgaattaa accaaaactt cttaatcgca ggggttaagg taagttcagc 60
taatcaatga aacttcccct ttcagcccgt tgaagaacca atg agc cct gcc cgc 115
Met Ser Pro Ala Arg
1 5
acc cag ccc cga gcg gct ttc cta agg aag ctc cga ccc atc aca tgt 163
Thr Gln Pro Arg Ala Ala Phe Leu Arg Lys Leu Arg Pro Ile Thr Cys
10 15 20
agc ctt tcc ttg gtt ctc gta ggg tgc agc aac acg gca gag tcc acg 211
Ser Leu Ser Leu Val Leu Val Gly Cys Ser Asn Thr Ala Glu Ser Thr
25 30 35
acc acc gat acg gca ggc tcc act gaa act tca tca cag att tca ccc 259
Thr Thr Asp Thr Ala Gly Ser Thr Glu Thr Ser Ser Gln Ile Ser Pro
40 45 50
acc act cca gtc agc acc acc gaa gca gcg ccg cct gaa gat cca agc 307
Thr Thr Pro Val Ser Thr Thr Glu Ala Ala Pro Pro Glu Asp Pro Ser
55 60 65
gca aac gct ttg gaa gag tat gct cag atc ctt gcc aat cca agc atc 355
Ala Asn Ala Leu Glu Glu Tyr Ala Gln Ile Leu Ala Asn Pro Ser Ile
70 75 80 85
tac ccc tta acc gaa att tca caa ttc gtc ccc aca ggc aca tat gcc 403
Tyr Pro Leu Thr Glu Ile Ser Gln Phe Val Pro Thr Gly Thr Tyr Ala
90 95 100
tac aca ctt gtt gaa gca act tcc gat tcc atc cca gaa cta cta ctc 451
Tyr Thr Leu Val Glu Ala Thr Ser Asp Ser Ile Pro Glu Leu Leu Leu
105 110 115
cgc gcc gat agt cac gaa ttc gcg cca att ttg gtt ttc acc tat gac 499
Arg Ala Asp Ser His Glu Phe Ala Pro Ile Leu Val Phe Thr Tyr Asp
120 125 130
gaa aac aca caa tcc gca acc caa gct gga gga gtc cta att gac ggt 547
Glu Asn Thr Gln Ser Ala Thr Gln Ala Gly Gly Val Leu Ile Asp Gly
135 140 145
gtc gcc agc gcc ggc ggt tca cga gtc aag gtc aga gct tca aat tca 595
Val Ala Ser Ala Gly Gly Ser Arg Val Lys Val Arg Ala Ser Asn Ser
150 155 160 165
gga gca gga atc tac caa ttg aat tgg tac tcg ata caa cca gtt ggc 643
Gly Ala Gly Ile Tyr Gln Leu Asn Trp Tyr Ser Ile Gln Pro Val Gly
170 175 180
gaa agc tct ttg tac gga atc caa gga aac tcc ctg aca caa atc gca 691
Glu Ser Ser Leu Tyr Gly Ile Gln Gly Asn Ser Leu Thr Gln Ile Ala
185 190 195
gac ccc gag gat ttc atg gtc cgt gaa cta tta cct gac cac cac gaa 739
Asp Pro Glu Asp Phe Met Val Arg Glu Leu Leu Pro Asp His His Glu
200 205 210
att acc tgg gtt gat tcc aac gac ccc tcc ggc cta rca acc gtt cag 787
Ile Thr Trp Val Asp Ser Asn Asp Pro Ser Gly Leu Ser Thr Val Gln
215 220 225
act ggt gga gcc aac att caa caa gca gcc cca act ccc gtt caa cag 835
Thr Gly Gly Ala Asn Ile Gln Gln Ala Ala Pro Thr Pro Val Gln Gln
230 235 240 245
cct gcg tca aac ctg cac tac ttc tct ggt gta gta acg atg cag acc 883
Pro Ala Ser Asn Leu His Tyr Phe Ser Gly Val Val Thr Met Gln Thr
250 255 260
gcc ggc gaa ctc atg cga ggt gaa cga act ccc aat ggt gag cct gcc 931
Ala Gly Glu Leu Met Arg Gly Glu Arg Thr Pro Asn Gly Glu Pro Ala
265 270 275
act gat ctc tat ctc gta ctg gtc ctt gat tcc cca att gaa atc aca 979
Thr Asp Leu Tyr Leu Val Leu Val Leu Asp Ser Pro Ile Glu Ile Thr
280 285 290
gca cga aac gca gct aca gac cct cag act cgt acc att tcc gaa gtc 1027
Ala Arg Asn Ala Ala Thr Asp Pro Gln Thr Arg Thr Ile Ser Glu Val
295 300 305
agc ctc ggc cga tac atc ccc gct gat ggt gac aat gat tgg ata ggc 1075
Ser Leu Gly Arg Tyr Ile Pro Ala Asp Gly Asp Asn Asp Trp Ile Gly
310 315 320 325
tac ctc gac acc cac gtg gaa atc act gca aca acg gat cag gtc tgg 1123
Tyr Leu Asp Thr His Val Glu Ile Thr Ala Thr Thr Asp Gln Val Trp
330 335 340
ttc cca acc gac acc ggt cta cct ctt gga atg ctt cgt cta gcg gac 1171
Phe Pro Thr Asp Thr Gly Leu Pro Leu Gly Met Leu Arg Leu Ala Asp
345 350 355
tac aaa agc atc tca taaaccgacg cagcactcgt cgataagcac 1216
Tyr Lys Ser Ile Ser
360
<210>174
<211>362
<212>PRT
<213>谷氨酸棒状杆菌
<400>174
Met Ser Pro Ala Arg Thr Gln Pro Arg Ala Ala Phe Leu Arg Lys Leu
1 5 10 15
Arg Pro Ile Thr Cys Ser Leu Ser Leu Val Leu Val Gly Cys Ser Asn
20 25 30
Thr Ala Glu Ser Thr Thr Thr Asp Thr Ala Gly Ser Thr Glu Thr Ser
35 40 45
Ser Gln Ile Ser Pro Thr Thr Pro Val Ser Thr Thr Glu Ala Ala Pro
50 55 60
Pro Glu Asp Pro Ser Ala Asn Ala Leu Glu Glu Tyr Ala Gln Ile Leu
65 70 75 80
Ala Asn Pro Ser Ile Tyr Pro Leu Thr Glu Ile Ser Gln Phe Val Pro
85 90 95
Thr Gly Thr Tyr Ala Tyr Thr Leu Val Glu Ala Thr Ser Asp Ser Ile
100 105 110
Pro Glu Leu Leu Leu Arg Ala Asp Ser His Glu Phe Ala Pro Ile Leu
115 120 125
Val Phe Thr Tyr Asp Glu Asn Thr Gln Ser Ala Thr Gln Ala Gly Gly
130 135 140
Val Leu Ile Asp Gly Val Ala Ser Ala Gly Gly Ser Arg Val Lys Val
145 150 155 160
Arg Ala Ser Asn Ser Gly Ala Gly Ile Tyr Gln Leu Asn Trp Tyr Ser
165 170 175
Ile Gln Pro Val Gly Glu Ser Ser Leu Tyr Gly Ile Gln Gly Asn Ser
180 185 190
Leu Thr Gln Ile Ala Asp Pro Glu Asp Phe Met Val Arg Glu Leu Leu
195 200 205
Pro Asp His His Glu Ile Thr Trp Val Asp Ser Asn Asp Pro Ser Gly
210 215 220
Leu Ser Thr Val Gln Thr Gly Gly Ala Asn Ile Gln Gln Ala Ala Pro
225 230 235 240
Thr Pro Val Gln Gln Pro Ala Ser Asn Leu His Tyr Phe Ser Gly Val
245 250 255
Val Thr Met Gln Thr Ala Gly Glu Leu Met Arg Gly Glu Arg Thr Pro
260 265 270
Asn Gly Glu Pro Ala Thr Asp Leu Tyr Leu Val Leu Val Leu Asp Ser
275 280 285
Pro Ile Glu Ile Thr Ala Arg Asn Ala Ala Thr Asp Pro Gln Thr Arg
290 295 300
Thr Ile Ser Glu Val Ser Leu Gly Arg Tyr Ile Pro Ala Asp Gly Asp
305 310 315 320
Asn Asp Trp Ile Gly Tyr Leu Asp Thr His Val Glu Ile Thr Ala Thr
325 330 335
Thr Asp Gln Val Trp Phe Pro Thr Asp Thr Gly Leu Pro Leu Gly Met
340 345 350
Leu Arg Leu Ala Asp Tyr Lys Ser Ile Ser
355 360
<210>175
<211>1381
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1351)
<223>RXA01642
<400>175
cttgaaaggt tggaatcact gtgtcttgca gctagtctgc cactcgcact gcatttgctg 60
gatgtatttg gaatgctgcg cggtcttgat attggattcg gtg gat aag atg cgc 115
Val Asp Lys Met Arg
1 5
aga ctc atc gcg gtt agc ttg gcc gct ctg ttt atg ttg gct tcc act 163
Arg Leu Ile Ala Val Ser Leu Ala Ala Leu Phe Met Leu Ala Ser Thr
10 15 20
cca gcg acg agg gca cag gaa gta gaa gct ctc gct tgc ccc gag gta 211
Pro Ala Thr Arg Ala Gln Glu Val Glu Ala Leu Ala Cys Pro Glu Val
25 30 35
gcg atc gcc gat cct tcc tcc gca gtt tta gat gaa cac ctt tcg cag 259
Ala Ile Ala Asp Pro Ser Ser Ala Val Leu Asp Glu His Leu Ser Gln
40 45 50
tca tta tcc caa gct cac caa cta gca act ggc gcc ggt gtg atg gtg 307
Ser Leu Ser Gln Ala His Gln Leu Ala Thr Gly Ala Gly Val Met Val
55 60 65
gca gtc atc gac acc gga gta tcc ctg cat cca cgt ctg ccc cac tta 355
Ala Val Ile Asp Thr Gly Val Ser Leu His Pro Arg Leu Pro His Leu
70 75 80 85
att ccc ggc ggt gat ttc gtg ggc gcc cac caa agc ccc gat gtg cca 403
Ile Pro Gly Gly Asp Phe Val Gly Ala His Gln Ser Pro Asp Val Pro
90 95 100
ggt gaa ctt atc gat tgc gac ggc cac ggc acc atc gtc gcc gga atc 451
Gly Glu Leu Ile Asp Cys Asp Gly His Gly Thr Ile Val Ala Gly Ile
105 110 115
atc gcc tcc caa gga aac ccc ggc acc ggc tgg cca tat gac ggc agc 499
Ile Ala Ser Gln Gly Asn Pro Gly Thr Gly Trp Pro Tyr Asp Gly Ser
120 125 130
tcc gat cct tat atc ggt gtc gcc cca gat tcc gga atc atc tcc att 547
Ser Asp Pro Tyr Ile Gly Val Ala Pro Asp Ser Gly Ile Ile Ser Ile
135 140 145
aaa caa acc agc tca tat gtg cgt act cgt gaa gat tcc aac gtc gga 595
Lys Gln Thr Ser Ser Tyr Val Arg Thr Arg Glu Asp Ser Asn Val Gly
150 155 160 165
acg ctg agc acc ctg gcg gaa tcc atc cac cga gct ctc gat tcc ggt 643
Thr Leu Ser Thr Leu Ala Glu Ser Ile His Arg Ala Leu Asp Ser Gly
170 175 180
gcc cac gtg atc aat att tcc gtg gtg tcc tgt ttg ccc caa tca ccc 691
Ala His Val Ile Asn Ile Ser Val Val Ser Cys Leu Pro Gln Ser Pro
185 190 195
gac gag gcc gca tcg ttc cag cct ctg acg gat gct ctt aac aga gca 739
Asp Glu Ala Ala Ser Phe Gln Pro Leu Thr Asp Ala Leu Asn Arg Ala
200 205 210
gaa ctt caa ggg gtg ata gtg gtg gca gca gca gga aac ctc ggg cag 787
Glu Leu Gln Gly Val Ile Val Val Ala Ala Ala Gly Asn Leu Gly Gln
215 220 225
gat tgt cca gtt gga tct acc gtt tat cct gca cat tca gac act gtg 835
Asp Cys Pro Val Gly Ser Thr Val Tyr Pro Ala His Ser Asp Thr Val
230 235 240 245
ctc tct gtg tcg gca cgt ttt gat tct cac acg ctt gca gaa tat tcc 883
Leu Ser Val Ser Ala Arg Phe Asp Ser His Thr Leu Ala Glu Tyr Ser
250 255 260
atg cct ggc aac caa caa atc ctc tct gca cca agc cac att cag gct 931
Met Pro Gly Asn Gln Gln Ile Leu Ser Ala Pro Ser His Ile Gln Ala
265 270 275
ggt cta tca ccg cgt ggc gac ggc ttc gcc agc cac atg atc acc acc 979
Gly Leu Ser Pro Arg Gly Asp Gly Phe Ala Ser His Met Ile Thr Thr
280 285 290
gct ggc gaa agc ccc ttc gag ggc acc agt ttt gcc gct cca gtt gtc 1027
Ala Gly Glu Ser Pro Phe Glu Gly Thr Ser Phe Ala Ala Pro Val Val
295 300 305
agc gcc aca gct gca ctg ctt cgc cag cat ttt ccc ttt gcc aca ccc 1075
Ser Ala Thr Ala Ala Leu Leu Arg Gln His Phe Pro Phe Ala Thr Pro
310 315 320 325
tat gaa att cgt gca cga atc ttc aac agc atc gac cct gca aga ggc 1123
Tyr Glu Ile Arg Ala Arg Ile Phe Asn Ser Ile Asp Pro Ala Arg Gly
330 335 340
gct att gat ccc tac ctg gca ctt act caa gaa atc tat ccc acc act 1171
Ala Ile Asp Pro Tyr Leu Ala Leu Thr Gln Glu Ile Tyr Pro Thr Thr
345 350 355
ccc ctg gtt cat gag atc gca cta agt gtt ccc acg ccg ccg gat gat 1219
Pro Leu Val His Glu Ile Ala Leu Ser Val Pro Thr Pro Pro Asp Asp
360 365 370
tct cca cgg gag cgg ggc atc cta gtt acc gca atc att gtt ggg ttg 1267
Ser Pro Arg Glu Arg Gly Ile Leu Val Thr Ala Ile Ile Val Gly Leu
375 380 385
ctc gca gtg tta gct gtg ctg atg gga cta cgc cga att cat cat cac 1315
Leu Ala Val Leu Ala Val Leu Met Gly Leu Arg Arg Ile His His His
390 395 400 405
tcg gcc ttt caa aaa gct agc tca agt gtt atc act taatctatga 1361
Ser Ala Phe Gln Lys Ala Ser Ser Ser Val Ile Thr
410 415
ggcaccgttc agaatcccat 1381
<210>176
<211>417
<212>PRT
<213>谷氨酸棒状杆菌
<400>176
Val Asp Lys Met Arg Arg Leu Ile Ala Val Ser Leu Ala Ala Leu Phe
1 5 10 15
Met Leu Ala Ser Thr Pro Ala Thr Arg Ala Gln Glu Val Glu Ala Leu
20 25 30
Ala Cys Pro Glu Val Ala Ile Ala Asp Pro Ser Ser Ala Val Leu Asp
35 40 45
Glu His Leu Ser Gln Ser Leu Ser Gln Ala His Gln Leu Ala Thr Gly
50 55 60
Ala Gly Val Met Val Ala Val Ile Asp Thr Gly Val Ser Leu His Pro
65 70 75 80
Arg Leu Pro His Leu Ile Pro Gly Gly Asp Phe Val Gly Ala His Gln
85 90 95
Ser Pro Asp Val Pro Gly Glu Leu Ile Asp Cys Asp Gly His Gly Thr
100 105 110
Ile Val Ala Gly Ile I1e Ala Ser Gln Gly Asn Pro Gly Thr Gly Trp
115 120 125
Pro Tyr Asp Gly Ser Ser Asp Pro Tyr Ile Gly Val Ala Pro Asp Ser
130 135 140
Gly Ile Ile Ser Ile Lys Gln Thr Ser Ser Tyr Val Arg Thr Arg Glu
145 150 155 160
Asp Ser Asn Val Gly Thr Leu Ser Thr Leu Ala Glu Ser Ile His Arg
165 170 175
Ala Leu Asp Ser Gly Ala His Val Ile Asn Ile Ser Val Val Ser Cys
180 185 190
Leu Pro Gln Ser Pro Asp Glu Ala Ala Ser Phe Gln Pro Leu Thr Asp
195 200 205
Ala Leu Asn Arg Ala Glu Leu Gln Gly Val Ile Val Val Ala Ala Ala
210 215 220
Gly Asn Leu Gly Gln Asp Cys Pro Val Gly Ser Thr Val Tyr Pro Ala
225 230 235 240
His Ser Asp Thr Val Leu Ser Val Ser Ala Arg Phe Asp Ser His Thr
245 250 255
Leu Ala Glu Tyr Ser Met Pro Gly Asn Gln Gln Ile Leu Ser Ala Pro
260 265 270
Ser His Ile Gln Ala Gly Leu Ser Pro Arg Gly Asp Gly Phe Ala Ser
275 280 285
His Met Ile Thr Thr Ala Gly Glu Ser Pro Phe Glu Gly Thr Ser Phe
290 295 300
Ala Ala Pro Val Val Ser Ala Thr Ala Ala Leu Leu Arg Gln His Phe
305 310 315 320
Pro Phe Ala Thr Pro Tyr Glu Ile Arg Ala Arg Ile Phe Asn Ser Ile
325 330 335
Asp Pro Ala Arg Gly Ala Ile Asp Pro Tyr Leu Ala Leu Thr Gln Glu
340 345 350
Ile Tyr Pro Thr Thr Pro Leu Val His Glu Ile Ala Leu Ser Val Pro
355 360 365
Thr Pro Pro Asp Asp Ser Pro Arg Glu Arg Gly Ile Leu Val Thr Ala
370 375 380
Ile Ile Val Gly Leu Leu Ala Val Leu Ala Val Leu Met Gly Leu Arg
385 390 395 400
Arg Ile His His His Ser Ala Phe Gln Lys Ala Ser Ser Ser Val Ile
405 410 415
Thr
<210>177
<211>1387
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1357)
<223>RXA01647
<400>177
gcaaatctga gaatcttccc actttcacta gtcttatccc ggaccagcgc actacagttc 60
ctggattagg ggatgtaaag atctaaagct gggggaatac atg gct gaa ggc atg 115
Met Ala Glu Gly Met
1 5
ttg atg ccc acg aca tcg gcg cag gtg tcg ggg cat aaa ttt ctg gtg 163
Leu Met Pro Thr Thr Ser Ala Gln Val Ser Gly His Lys Phe Leu Val
10 15 20
cgg cgc atc gaa cat ggg ttg gtg atg ggc gat gtg cgc atg att cat 211
Arg Arg Ile Glu His Gly Leu Val Met Gly Asp Val Arg Met Ile His
25 30 35
gat cca ctt ggt agg cgc cgg cgg gcg ttg gtg ttt ggg gtg gtg gcg 259
Asp Pro Leu Gly Arg Arg Arg Arg Ala Leu Val Phe Gly Val Val Ala
40 45 50
tgc gtg atg ttg gcg gtg gga tca ttg gcg ttg gct att ttt cga ccc 307
Cys Val Met Leu Ala Val Gly Ser Leu Ala Leu Ala Ile Phe Arg Pro
55 60 65
gcg aag gat ccg gcc gat gcg ccg ttg atc cgc tct gaa tcc ggc gcg 355
Ala Lys Asp Pro Ala Asp Ala Pro Leu Ile Arg Ser Glu Ser Gly Ala
70 75 80 85
ctc ttt gtg cag ctg gat ggg tcg gtg cat ccg gtg gct aat gtg gcc 403
Leu Phe Val Gln Leu Asp Gly Ser Val His Pro Val Ala Asn Val Ala
90 95 100
tcg gct cgg ttg att gtg ggg gag ccg gtg gat ccg gtg aac gcc agc 451
Ser Ala Arg Leu Ile Val Gly Glu Pro Val Asp Pro Val Asn Ala Ser
105 110 115
gat gcg atc atc gcg ggc atg ccg cgc gga gtg ccg gtg ggg gtt cct 499
Asp Ala Ile Ile Ala Gly Met Pro Arg Gly Val Pro Val Gly Val Pro
120 125 130
gat gcg ccg ggg ctt ttc agc agc acc gaa gaa ccc gag caa gat tgg 547
Asp Ala Pro Gly Leu Phe Ser Ser Thr Glu Glu Pro Glu Gln Asp Trp
135 140 145
ttt gtg tgc cag gat gtc ggc act ggg gat cta cac att acg gtt cct 595
Phe Val Cys Gln Asp Val Gly Thr Gly Asp Leu His Ile Thr Val Pro
150 155 160 165
agg ggc gga cta ggg ccc acc ctg att gcg gaa gga aat ggg tgg ctg 643
Arg Gly Gly Leu Gly Pro Thr Leu Ile Ala Glu Gly Asn Gly Trp Leu
170 175 180
ggg gcg tcg aaa agc gaa acc ggc gag gtc acc tgg aac ctg att acc 691
Gly Ala Ser Lys Ser Glu Thr Gly Glu Val Thr Trp Asn Leu Ile Thr
185 190 195
gcg gac ggg cgc cgc gaa ctg ccg gcg tgg ggc agc gaa cat ggg cgc 739
Ala Asp Gly Arg Arg Glu Leu Pro Ala Trp Gly Ser Glu His Gly Arg
200 205 210
att atg cgc cgc cac ctg ggg att tcc gag gac acc ccg cgc gta tac 787
Ile Met Arg Arg His Leu Gly Ile Ser Glu Asp Thr Pro Arg Val Tyr
215 220 225
ctg acc act gag ctg ctc aac gcg atc ccc gag cac gac gcg gtc cgc 835
Leu Thr Thr Glu Leu Leu Asn Ala Ile Pro Glu His Asp Ala Val Arg
230 235 240 245
ttc cca gcc ccg ctg ccc gag ctt gtc gac gcc tcc acc cgc aac tgg 883
Phe Pro Ala Pro Leu Pro Glu Leu Val Asp Ala Ser Thr Arg Asn Trp
250 255 260
tta cgg ctc gac ggg gcg ctc gcc gaa atc acg ccg cta cag cgc ggg 931
Leu Arg Leu Asp Gly Ala Leu Ala Glu Ile Thr Pro Leu Gln Arg Gly
265 270 275
ttg ctt atc gac gcc ggt tcc ggt gtt ttc ccc gac ccc acc gcg ctt 979
Leu Leu Ile Asp Ala Gly Ser Gly Val Phe Pro Asp Pro Thr Ala Leu
280 285 290
ctt ggt gtg cat gaa gaa aca gcc aac acc ttg acg ctg ccc gag caa 1027
Leu Gly Val His Glu Glu Thr Ala Asn Thr Leu Thr Leu Pro Glu Gln
295 300 305
aca gtt tct tgg caa gat ctg gac ggt ggt ttt gcc tgc gcg gat ggt 1075
Thr Val Ser Trp Gln Asp Leu Asp Gly Gly Phe Ala Cys Ala Asp Gly
310 315 320 325
gaa ggc cag atc ggt ttc ctg gaa act ctg gaa tcg ggg gtg gcg cta 1123
Glu Gly Gln Ile Gly Phe Leu Glu Thr Leu Glu Ser Gly Val Ala Leu
330 335 340
tct ggt gat tcc agg gcg aaa agt ttc agc aca aac gct ggt ggg gca 1171
Ser Gly Asp Ser Arg Ala Lys Ser Phe Ser Thr Asn Ala Gly Gly Ala
345 350 355
gtg ggc gtg gac agt ggc ttt ggc tac tat gtg gtc tct gat ttt ggg 1219
Val Gly Val Asp Ser Gly Phe Gly Tyr Tyr Val Val Ser Asp Phe Gly
360 365 370
ctg atg cac cct gtt tct act ggt gaa tcg atg gtt gcc cta gga atc 1267
Leu Met His Pro Val Ser Thr Gly Glu Ser Met Val Ala Leu Gly Ile
375 380 385
act gac gtg cag gtc gtg ccg tgg agc gtg ctg cga ttg ttg ccg cag 1315
Thr Asp Val Gln Val Val Pro Trp Ser Val Leu Arg Leu Leu Pro Gln
390 395 400 405
gga agt gaa tta gca aaa gag aca gcg ctc gcg ccc acc tat 1357
Gly Ser Glu Leu Ala Lys Glu Thr Ala Leu Ala Pro Thr Tyr
410 415
taaggagtat ggtggcctta catgagcgga 1387
<210>178
<211>419
<212>PRT
<213>谷氨酸棒状杆菌
<400>178
Met Ala Glu Gly Met Leu Met Pro Thr Thr Ser Ala Gln Val Ser Gly
1 5 10 15
His Lys Phe Leu Val Arg Arg Ile Glu His Gly Leu Val Met Gly Asp
20 25 30
Val Arg Met Ile His Asp Pro Leu Gly Arg Arg Arg Arg Ala Leu Val
35 40 45
Phe Gly Val Val Ala Cys Val Met Leu Ala Val Gly Ser Leu Ala Leu
50 55 60
Ala Ile Phe Arg Pro Ala Lys Asp Pro Ala Asp Ala Pro Leu Ile Arg
65 70 75 80
Ser Glu Ser Gly Ala Leu Phe Val Gln Leu Asp Gly Ser Val His Pro
85 90 95
Val Ala Asn Val Ala Ser Ala Arg Leu Ile Val Gly Glu Pro Val Asp
100 105 110
Pro Val Asn Ala Ser Asp Ala Ile Ile Ala Gly Met Pro Arg Gly Val
115 120 125
Pro Val Gly Val Pro Asp Ala Pro Gly Leu Phe Ser Ser Thr Glu Glu
130 135 140
Pro Glu Gln Asp Trp Phe Val Cys Gln Asp Val Gly Thr Gly Asp Leu
145 150 155 160
His Ile Thr Val Pro Arg Gly Gly Leu Gly Pro Thr Leu Ile Ala Glu
165 170 175
Gly Asn Gly Trp Leu Gly Ala Ser Lys Ser Glu Thr Gly Glu Val Thr
180 185 190
Trp Asn Leu Ile Thr Ala Asp Gly Arg Arg Glu Leu Pro Ala Trp Gly
195 200 205
Ser Glu His Gly Arg Ile Met Arg Arg His Leu Gly Ile Ser Glu Asp
210 215 220
Thr Pro Arg Val Tyr Leu Thr Thr Glu Leu Leu Asn Ala Ile Pro Glu
225 230 235 240
His Asp Ala Val Arg Phe Pro Ala Pro Leu Pro Glu Leu Val Asp Ala
245 250 255
Ser Thr Arg Asn Trp Leu Arg Leu Asp Gly Ala Leu Ala Glu Ile Thr
260 265 270
Pro Leu Gln Arg Gly Leu Leu Ile Asp Ala Gly Ser Gly Val Phe Pro
275 280 285
Asp Pro Thr Ala Leu Leu Gly Val His Glu Glu Thr Ala Asn Thr Leu
290 295 300
Thr Leu Pro Glu Gln Thr Val Ser Trp Gln Asp Leu Asp Gly Gly Phe
305 310 315 320
Ala Cys Ala Asp Gly Glu Gly Gln Ile Gly Phe Leu Glu Thr Leu Glu
325 330 335
Ser Gly Val Ala Leu Ser Gly Asp Ser Arg Ala Lys Ser Phe Ser Thr
340 345 350
Asn Ala Gly Gly Ala Val Gly Val Asp Ser Gly Phe Gly Tyr Tyr Val
355 360 365
Val Ser Asp Phe Gly Leu Met His Pro Val Ser Thr Gly Glu Ser Met
370 375 380
Val Ala Leu Gly Ile Thr Asp Val Gln Val Val Pro Trp Ser Val Leu
385 390 395 400
Arg Leu Leu Pro Gln Gly Ser Glu Leu Ala Lys Glu Thr Ala Leu Ala
405 410 415
Pro Thr Tyr
<210>179
<211>472
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(442)
<223>RXA01672
<400>179
agtagcaaag ggtgcagtcg aaggaccagc atccaagact gctgccgaag aaaccgactc 60
cgttgacaac ggatccgacg aaaacggcga ggaataattt atg gca tcc cga gaa 115
Met Ala Ser Arg Glu
1 5
gta tcc atc acc cga atc tca cca cta gca acc ttc cga gtt gca ctg 163
Val Ser Ile Thr Arg Ile Ser Pro Leu Ala Thr Phe Arg Val Ala Leu
10 15 20
gca atg tcc atc atc gga ctc gtg gcg tgg atc atc tgc gta acc gtc 211
Ala Met Ser Ile Ile Gly Leu Val Ala Trp Ile Ile Cys Val Thr Val
25 30 35
ctc tat ttc gga ctt aac gtg gct ggc gtc tgg cag aac ttc aac gat 259
Leu Tyr Phe Gly Leu Asn Val Ala Gly Val Trp Gln Asn Phe Asn Asp
40 45 50
gtc atc ggc ggt gtt ggt gca gaa caa acc atc acc ttc ggg ctc gtc 307
Val Ile Gly Gly Val Gly Ala Glu Gln Thr Ile Thr Phe Gly Leu Val
55 60 65
ctg agc att tcc gca ctt ctg gga gct atc gga gcg att acc gtc gct 355
Leu Ser Ile Ser Ala Leu Leu Gly Ala Ile Gly Ala Ile Thr Val Ala
70 75 80 85
gtg ctt gca cca ttg tgt gca atc atc tac aac tcg att gtt gac ctc 403
Val Leu Ala Pro Leu Cys Ala Ile Ile Tyr Asn Ser Ile Val Asp Leu
90 95 100
ttc ggt gga ctg cag att caa ctg caa gaa gaa gta gac taacctctga 452
Phe Gly Gly Leu Gln Ile Gln Leu Gln Glu Glu Val Asp
105 110
aacacaaacc cctagacact 472
<210>180
<211>114
<212>PRT
<213>谷氨酸棒状杆菌
<400>180
Met Ala Ser Arg Glu Val Ser Ile Thr Arg Ile Ser Pro Leu Ala Thr
1 5 10 15
Phe Arg Val Ala Leu Ala Met Ser Ile Ile Gly Leu Val Ala Trp Ile
20 25 30
Ile Cys Val Thr Val Leu Tyr Phe Gly Leu Asn Val Ala Gly Val Trp
35 40 45
Gln Asn Phe Asn Asp Val Ile Gly Gly Val Gly Ala Glu Gln Thr Ile
50 55 60
Thr Phe Gly Leu Val Leu Ser Ile Ser Ala Leu Leu Gly Ala Ile Gly
65 70 75 80
Ala Ile Thr Val Ala Val Leu Ala Pro Leu Cys Ala Ile Ile Tyr Asn
85 90 95
Ser Ile Val Asp Leu Phe Gly Gly Leu Gln Ile Gln Leu Gln Glu Glu
100 105 110
Val Asp
<210>181
<211>874
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(844)
<223>RXA01677
<400>181
gtcgccatag ttgagtttta ttcatggctt ttagctaggc gactttagtt gagggctttt 60
agttgagggc ttcccagcag ggatggttaa ggagaattca gtg aac caa cag agt 115
Val Asn Gln Gln Ser
1 5
aaa aag tgg ctc gta ccg aca ctg gtc gtc atc att gca gtg ctc ctc 163
Lys Lys Trp Leu Val Pro Thr Leu Val Val Ile Ile Ala Val Leu Leu
10 15 20
atc gca gtt gtt ctg ttg atg tac cga gga aat gcg agt gat acg gcc 211
Ile Ala Val Val Leu Leu Met Tyr Arg Gly Asn Ala Ser Asp Thr Ala
25 30 35
gag ggc gtt tca gcc gct gcg act tcg gac tcg gct gct gct tcg act 259
Glu Gly Val Ser Ala Ala Ala Thr Ser Asp Ser Ala Ala Ala Ser Thr
40 45 50
gct gct tcg ggt tcc gct tct ggt gct gcg gac tcc gat ctg acc agc 307
Ala Ala Ser Gly Ser Ala Ser Gly Ala Ala Asp Ser Asp Leu Thr Ser
55 60 65
gtg gaa gca cgc gac cct tcc gac cct gtt gcg gtg gga gac gtt gat 355
Val Glu Ala Arg Asp Pro Ser Asp Pro Val Ala Val Gly Asp Val Asp
70 75 80 85
gca cct gtt ggg tta gtg gtg ttt tcc gac tac caa tgc ccg ttc tgt 403
Ala Pro Val Gly Leu Val Val Phe Ser Asp Tyr Gln Cys Pro Phe Cys
90 95 100
gca aag tgg agc gat gaa acc ctg cca cag atg atg aag cat gtg gaa 451
Ala Lys Trp Ser Asp Glu Thr Leu Pro Gln Met Met Lys His Val Glu
105 110 115
gat gga aac ctc cgc att gaa tgg cgt gaa gtg aac atc ttt gga gaa 499
Asp Gly Asn Leu Arg Ile Glu Trp Arg Glu Val Asn Ile Phe Gly Glu
120 125 130
cca tct gag cgt gga gct cgc gcg gca tac gct gcg ggt ttg cag gac 547
Pro Ser Glu Arg Gly Ala Arg Ala Ala Tyr Ala Ala Gly Leu Gln Asp
135 140 145
gca tac ttg gaa tac cac aac gca ctc ttt gcc aac ggt gaa aaa ccc 595
Ala Tyr Leu Glu Tyr His Asn Ala Leu Phe Ala Asn Gly Glu Lys Pro
150 155 160 165
agc gaa gac ctg ctc agc gaa gag gga ctt att aag ctt gct ggt gac 643
Ser Glu Asp Leu Leu Ser Glu Glu Gly Leu Ile Lys Leu Ala Gly Asp
170 175 180
ctt gga cta gac gaa tcg aaa ttc act gcc gat ttc caa tcc cct gaa 691
Leu Gly Leu Asp Glu Ser Lys Phe Thr Ala Asp Phe Gln Ser Pro Glu
185 190 195
act gca gtc gca att gcg caa cat caa cag ctg gga atc gat ctt ggc 739
Thr Ala Val Ala Ile Ala Gln His Gln Gln Leu Gly Ile Asp Leu Gly
200 205 210
gcc tac tcc acc cca gct ttc ctc cta ggt ggc cag cca atc atg ggc 787
Ala Tyr Ser Thr Pro Ala Phe Leu Leu Gly Gly Gln Pro Ile Met Gly
215 220 225
gct cag cct gct tct gta ttt gaa gcc gcc ttc gag caa gca ctg gca 835
Ala Gln Pro Ala Ser Val Phe Glu Ala Ala Phe Glu Gln Ala Leu Ala
230 235 240 245
gcg aaa gaa taaaccgtgg atgtcggcct agttacagct 874
Ala Lys Glu
<210>182
<211>248
<212>PRT
<213>谷氨酸棒状杆菌
<400>182
Val Asn Gln Gln Ser Lys Lys Trp Leu Val Pro Thr Leu Val Val Ile
1 5 10 15
Ile Ala Val Leu Leu Ile Ala Val Val Leu Leu Met Tyr Arg Gly Asn
20 25 30
Ala Ser Asp Thr Ala Glu Gly Val Ser Ala Ala Ala Thr Ser Asp Ser
35 40 45
Ala Ala Ala Ser Thr Ala Ala Ser Gly Ser Ala Ser Gly Ala Ala Asp
50 55 60
Ser Asp Leu Thr Ser Val Glu Ala Arg Asp Pro Ser Asp Pro Val Ala
65 70 75 80
Val Gly Asp Val Asp Ala Pro Val Gly Leu Val Val Phe Ser Asp Tyr
85 90 95
Gln Cys Pro Phe Cys Ala Lys Trp Ser Asp Glu Thr Leu Pro Gln Met
100 105 110
Met Lys His Val Glu Asp Gly Asn Leu Arg Ile Glu Trp Arg Glu Val
115 120 125
Asn Ile Phe Gly Glu Pro Ser Glu Arg Gly Ala Arg Ala Ala Tyr Ala
130 135 140
Ala Gly Leu Gln Asp Ala Tyr Leu Glu Tyr His Asn Ala Leu Phe Ala
145 150 155 160
Asn Gly Glu Lys Pro Ser Glu Asp Leu Leu Ser Glu Glu Gly Leu Ile
165 170 175
Lys Leu Ala Gly Asp Leu Gly Leu Asp Glu Ser Lys Phe Thr Ala Asp
180 185 190
Phe Gln Ser Pro Glu Thr Ala Val Ala Ile Ala Gln His Gln Gln Leu
195 200 205
Gly Ile Asp Leu Gly Ala Tyr Ser Thr Pro Ala Phe Leu Leu Gly Gly
2l0 215 220
Gln Pro Ile Met Gly Ala Gln Pro Ala Ser Val Phe Glu Ala Ala Phe
225 230 235 240
Glu Gln Ala Leu Ala Ala Lys Glu
245
<210>183
<211>1552
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1522)
<223>RXA01693
<400>183
aggagactgg ctggattatt ggctggtttt cttgggaaat cgtcatgggc attaatccta 60
gtcccaacaa ttgccagaac ccgaacacta ggcttgaacc atg aat act gca cct 115
Met Asn Thr Ala Pro
1 5
ttc aaa ctc gaa gct gac ttc gca tca gcc ctg ccc acc atg gca gcc 163
Phe Lys Leu Glu Ala Asp Phe Ala Ser Ala Leu Pro Thr Met Ala Ala
10 15 20
ccc tgg caa ggt gag gaa gcc ccc aac cct gag ctc gtg att tta aat 211
Pro Trp Gln Gly Glu Glu Ala Pro Asn Pro Glu Leu Val Ile Leu Asn
25 30 35
gac gac ctc gcc tac agc ctc ggg ctt gat ccg aca tgg ctt cgc aca 259
Asp Asp Leu Ala Tyr Ser Leu Gly Leu Asp Pro Thr Trp Leu Arg Thr
40 45 50
cct gag ggc gtt caa ttt ctt ctc gga ctc aac ccc gag ccc tta aca 307
Pro Glu Gly Val Gln Phe Leu Leu Gly Leu Asn Pro Glu Pro Leu Thr
55 60 65
aaa gca gtt gcg cag gcc tat tcc ggc cac caa ttc gga cag ttt gtg 355
Lys Ala Val Ala Gln Ala Tyr Ser Gly His Gln Phe Gly Gln Phe Val
70 75 80 85
gca agc ctt ggt gat ggc cga gcg ctt ctt ctc ggc gaa gcc cgc tca 403
Ala Ser Leu Gly Asp Gly Arg Ala Leu Leu Leu Gly Glu Ala Arg Ser
90 95 100
gct gac ggc gta ctg cat gat atc cac ctc aaa gga tct gga cga acc 451
Ala Asp Gly Val Leu His Asp Ile His Leu Lys Gly Ser Gly Arg Thr
105 110 115
caa ttc tcc cga gga gcc gat gga cgc gcc gtc ctt ggc ccc gtc tta 499
Gln Phe Ser Arg Gly Ala Asp Gly Arg Ala Val Leu Gly Pro Val Leu
120 125 130
cgc gaa tac atc atc tcc gaa gcg atg cat gca ctt ggt gtt ccc acc 547
Arg Glu Tyr Ile Ile Ser Glu Ala Met His Ala Leu Gly Val Pro Thr
135 140 145
acc agg tca ctt gca gta att agc acc ggt agg aaa atc caa cga gga 595
Thr Arg Ser Leu Ala Val Ile Ser Thr Gly Arg Lys Ile Gln Arg Gly
150 155 160 165
agc gta gcc cca ggc gca gtc ctt gtt cga gta gca acc agc ctc att 643
Ser Val Ala Pro Gly Ala Val Leu Val Arg Val Ala Thr Ser Leu Ile
170 175 180
cga gtc gga tcc ttc caa tac tcc aac atc tct ggt ggc atc gaa cta 691
Arg Val Gly Ser Phe Gln Tyr Ser Asn Ile Ser Gly Gly Ile Glu Leu
185 190 195
tct caa cac ctg gcg aac tat acg atc acc agg cat ttc cct tcg ttg 739
Ser Gln His Leu Ala Asn Tyr Thr Ile Thr Arg His Phe Pro Ser Leu
200 205 210
gta gct gaa cta tcc gca cca acc ccc gca act tat gta tca ctg ttt 787
Val Ala Glu Leu Ser Ala Pro Thr Pro Ala Thr Tyr Val Ser Leu Phe
215 220 225
aaa gcg att ctt cag cgc caa gca gac acc gtt gga aaa tgg acc agg 835
Lys Ala Ile Leu Gln Arg Gln Ala Asp Thr Val Gly Lys Trp Thr Arg
230 235 240 245
ctg ggt ttc gtt cac gga gcc ctc aac aca gac aac acg ttg ata tcc 883
Leu Gly Phe Val His Gly Ala Leu Asn Thr Asp Asn Thr Leu Ile Ser
250 255 260
gga gaa act gtt gac tac ggc cca tgc gct ttc atg gag cgc tac cgt 931
Gly Glu Thr Val Asp Tyr Gly Pro Cys Ala Phe Met Glu Arg Tyr Arg
265 270 275
ggc gac gcg aaa ttt agc tcc atc gac act tat ggt cgc tac aaa ttt 979
Gly Asp Ala Lys Phe Ser Ser Ile Asp Thr Tyr Gly Arg Tyr Lys Phe
280 285 290
gaa aac caa cct atg atc ctc gga tgg aac atg gcc cgc ctc gta gaa 1027
Glu Asn Gln Pro Met Ile Leu Gly Trp Asn Met Ala Arg Leu Val Glu
295 300 305
acc ctc ctc cca ctc ctg ggc gcc aca cca gac gaa ggc atg aca gca 1075
Thr Leu Leu Pro Leu Leu Gly Ala Thr Pro Asp Glu Gly Met Thr Ala
310 315 320 325
gcc caa gaa gct ctc gta gaa ttc gat gac ctc tgc gaa caa gca atc 1123
Ala Gln Glu Ala Leu Val Glu Phe Asp Asp Leu Cys Glu Gln Ala Ile
330 335 340
cga aaa gaa ttc gcc act gca ctg ggc ctt gac gag tca gac acc ggc 1171
Arg Lys Glu Phe Ala Thr Ala Leu Gly Leu Asp Glu Ser Asp Thr Gly
345 350 355
acg gta gag cag ttc cgt gaa ctg ctc tac ctc cat aac ccc gac atc 1219
Thr Val Glu Gln Phe Arg Glu Leu Leu Tyr Leu His Asn Pro Asp Ile
360 365 370
acc acg ctg ctg cgc gca ctc acc gac aac acc gca cca ccg agt ggc 1267
Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr Ala Pro Pro Ser Gly
375 380 385
ttt gaa gca ttc gtt cac gac tgg aaa acc caa gac cca gat atc gaa 1315
Phe Glu Ala Phe Val His Asp Trp Lys Thr Gln Asp Pro Asp Ile Glu
390 395 400 405
gca atg cga gca gta aat cca ctt ttc att cca cgc aat cac ctc gtg 1363
Ala Met Arg Ala Val Asn Pro Leu Phe Ile Pro Arg Asn His Leu Val
410 415 420
gaa gct gct ctc gca gac gca gtt gaa ggg aat cta gaa aag ttc cac 1411
Glu Ala Ala Leu Ala Asp Ala Val Glu Gly Asn Leu Glu Lys Phe His
425 430 435
gaa ctc ctc gct gct gtc acc aat cct ttt gat cca act gcg ggc ccc 1459
Glu Leu Leu Ala Ala Val Thr Asn Pro Phe Asp Pro Thr Ala Gly Pro
440 445 450
gat gaa cta cgc ctg cca agc gaa gaa gga ttt gaa gaa gac tac atg 1507
Asp Glu Leu Arg Leu Pro Ser Glu Glu Gly Phe Glu Glu Asp Tyr Met
455 460 465
acc ttc tgc ggt acc taggacagat ggtggggcag acgtcggggt 1552
Thr Phe Cys Gly Thr
470
<210>184
<211>474
<212>PRT
<213>谷氨酸棒状杆菌
<400>184
Met Asn Thr Ala Pro Phe Lys Leu Glu Ala Asp Phe Ala Ser Ala Leu
1 5 10 15
Pro Thr Met Ala Ala Pro Trp Gln Gly Glu Glu Ala Pro Asn Pro Glu
20 25 30
Leu Val Ile Leu Asn Asp Asp Leu Ala Tyr Ser Leu Gly Leu Asp Pro
35 40 45
Thr Trp Leu Arg Thr Pro Glu Gly Val Gln Phe Leu Leu Gly Leu Asn
50 55 60
Pro Glu Pro Leu Thr Lys Ala Val Ala Gln Ala Tyr Ser Gly His Gln
65 70 75 80
Phe Gly Gln Phe Val Ala Ser Leu Gly Asp Gly Arg Ala Leu Leu Leu
85 90 95
Gly Glu Ala Arg Ser Ala Asp Gly Val Leu His Asp Ile His Leu Lys
100 105 110
Gly Ser Gly Arg Thr Gln Phe Ser Arg Gly Ala Asp Gly Arg Ala Val
115 120 125
Leu Gly Pro Val Leu Arg Glu Tyr Ile Ile Ser Glu Ala Met His Ala
130 135 140
Leu Gly Val Pro Thr Thr Arg Ser Leu Ala Val Ile Ser Thr Gly Arg
145 150 155 160
Lys Ile Gln Arg Gly Ser Val Ala Pro Gly Ala Val Leu Val Arg Val
165 170 175
Ala Thr Ser Leu Ile Arg Val Gly Ser Phe Gln Tyr Ser Asn Ile Ser
180 185 190
Gly Gly Ile Glu Leu Ser Gln His Leu Ala Asn Tyr Thr Ile Thr Arg
195 200 205
His Phe Pro Ser Leu Val Ala Glu Leu Ser Ala Pro Thr Pro Ala Thr
210 215 220
Tyr Val Ser Leu Phe Lys Ala Ile Leu Gln Arg Gln Ala Asp Thr Val
225 230 235 240
Gly Lys Trp Thr Arg Leu Gly Phe Val His Gly Ala Leu Asn Thr Asp
245 250 255
Asn Thr Leu Ile Ser Gly Glu Thr Val Asp Tyr Gly Pro Cys Ala Phe
260 265 270
Met Glu Arg Tyr Arg Gly Asp Ala Lys Phe Ser Ser Ile Asp Thr Tyr
275 280 285
Gly Arg Tyr Lys Phe Glu Asn Gln Pro Met Ile Leu Gly Trp Asn Met
290 295 300
Ala Arg Leu Val Glu Thr Leu Leu Pro Leu Leu Gly Ala Thr Pro Asp
305 310 315 320
Glu Gly Met Thr Ala Ala Gln Glu Ala Leu Val Glu Phe Asp Asp Leu
325 330 335
Cys Glu Gln Ala Ile Arg Lys Glu Phe Ala Thr Ala Leu Gly Leu Asp
340 345 350
Glu Ser Asp Thr Gly Thr Val Glu Gln Phe Arg Glu Leu Leu Tyr Leu
355 360 365
His Asn Pro Asp Ile Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr
370 375 380
Ala Pro Pro Ser Gly Phe Glu Ala Phe Val His Asp Trp Lys Thr Gln
385 390 395 400
Asp Pro Asp Ile Glu Ala Met Arg Ala Val Asn Pro Leu Phe Ile Pro
405 410 415
Arg Asn His Leu Val Glu Ala Ala Leu Ala Asp Ala Val Glu Gly Asn
420 425 430
Leu Glu Lys Phe His Glu Leu Leu Ala Ala Val Thr Asn Pro Phe Asp
435 440 445
Pro Thr Ala Gly Pro Asp Glu Leu Arg Leu Pro Ser Glu Glu Gly Phe
450 455 460
Glu Glu Asp Tyr Met Thr Phe Cys Gly Thr
465 470
<210>185
<211>928
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(898)
<223>RXA01741
<400>185
gcatctctgg cttttcctgc atgacagatg attgtaactg tgatgaaata aggccagatg 60
aagtaactgt gtcgattaag ttttggggga aatttttcag atg aca cac atc gct 115
Met Thr His Ile Ala
1 5
tta gcc aac tct gcc caa gtt ctg atc cgc ccg gat tct gcg att caa 163
Leu Ala Asn Ser Ala Gln Val Leu Ile Arg Pro Asp Ser Ala Ile Gln
10 15 20
ttc ggc atc gac gcc acc cgc gct ggc gtc tta aat atc gat cca tcg 211
Phe Gly Ile Asp Ala Thr Arg Ala Gly Val Leu Asn Ile Asp Pro Ser
25 30 35
ctg tcg tcg cga gtc gtc ccg gtg ctg cgg aat ctg cgg acc gcc cga 259
Leu Ser Ser Arg Val Val Pro Val Leu Arg Asn Leu Arg Thr Ala Arg
40 45 50
ccg atc gtt gat gtc atc gcc gac ctc acg act gca ggc ctc gca ccc 307
Pro Ile Val Asp Val Ile Ala Asp Leu Thr Thr Ala Gly Leu Ala Pro
55 60 65
acc gct gcg agc agt ttg ctc gag gac ctt tta gaa ttc ggt gtg gtc 355
Thr Ala Ala Ser Ser Leu Leu Glu Asp Leu Leu Glu Phe Gly Val Val
70 75 80 85
cgc gaa tcg gcg gcg gcg cag gtg ttg ctg ttc ggg gac ggt tcg ctt 403
Arg Glu Ser Ala Ala Ala Gln Val Leu Leu Phe Gly Asp Gly Ser Leu
90 95 100
gtc gac gtc acc tcc ttc ctt ttg gaa acc tcc ggc ttt gtt ccc aga 451
Val Asp Val Thr Ser Phe Leu Leu Glu Thr Ser Gly Phe Val Pro Arg
105 110 115
ccc cag atc atc gat gag tcg cct cga gag ttt ttc gag ctt ccc tcc 499
Pro Gln Ile Ile Asp Glu Ser Pro Arg Glu Phe Phe Glu Leu Pro Ser
120 125 130
agc cac att ttg gtt ctc aac aag ctc gca cat tcc caa cgt cta tcc 547
Ser His Ile Leu Val Leu Asn Lys Leu Ala His Ser Gln Arg Leu Ser
135 140 145
ccg ctg ctt cac aaa tat gcg ccg acg tat ctg tgc gcc gcg atc gtc 595
Pro Leu Leu His Lys Tyr Ala Pro Thr Tyr Leu Cys Ala Ala Ile Val
150 155 160 165
gat aat cgt ggc atc atc ggc ccg ggc cgg aga tca cga tcg ggg ccg 643
Asp Asn Arg Gly Ile Ile Gly Pro Gly Arg Arg Ser Arg Ser Gly Pro
170 175 180
tgt ttg atg tgt gtg gat ctg cat cgc tgc gat atc gat ccg cat tgg 691
Cys Leu Met Cys Val Asp Leu His Arg Cys Asp Ile Asp Pro His Trp
185 190 195
ctc tct att atc aat cag caa ccc aac ggt ccc acc ttt cct gat ccc 739
Leu Ser Ile Ile Asn Gln Gln Pro Asn Gly Pro Thr Phe Pro Asp Pro
200 205 210
gtc acg gag atg gcg acg gct gcc cga ctc gtc gcc tgg gtc act gcc 787
Val Thr Glu Met Ala Thr Ala Ala Arg Leu Val Ala Trp Val Thr Ala
215 220 225
gat aca tgg ttg ccc ggc gtt gtg gag gaa gta aac ccc cac gat cga 835
Asp Thr Trp Leu Pro Gly Val Val Glu Glu Val Asn Pro His Asp Arg
230 235 240 245
aca aac tcg gta cgc acc ctc cct gtg cat cca aaa tgt ccg atg tgt 883
Thr Asn Ser Val Arg Thr Leu Pro Val His Pro Lys Cys Pro Met Cys
250 255 260
tgg agt tta ggt tcc taaagtgctc ccaaaatcgc gagcacgtca 928
Trp Ser Leu Gly Ser
265
<210>186
<211>266
<212>PRT
<213>谷氨酸棒状杆菌
<400>186
Met Thr His Ile Ala Leu Ala Asn Ser Ala Gln Val Leu Ile Arg Pro
1 5 10 15
Asp Ser Ala Ile Gln Phe Gly Ile Asp Ala Thr Arg Ala Gly Val Leu
20 25 30
Asn Ile Asp Pro Ser Leu Ser Ser Arg Val Val Pro Val Leu Arg Asn
35 40 45
Leu Arg Thr Ala Arg Pro Ile Val Asp Val Ile Ala Asp Leu Thr Thr
50 55 60
Ala Gly Leu Ala Pro Thr Ala Ala Ser Ser Leu Leu Glu Asp Leu Leu
65 70 75 80
Glu Phe Gly Val Val Arg Glu Ser Ala Ala Ala Gln Val Leu Leu Phe
85 90 95
Gly Asp Gly Ser Leu Val Asp Val Thr Ser Phe Leu Leu Glu Thr Ser
100 105 110
Gly Phe Val Pro Arg Pro Gln Ile Ile Asp Glu Ser Pro Arg Glu Phe
115 120 125
Phe Glu Leu Pro Ser Ser His Ile Leu Val Leu Asn Lys Leu Ala His
130 135 140
Ser Gln Arg Leu Ser Pro Leu Leu His Lys Tyr Ala Pro Thr Tyr Leu
145 150 155 160
Cys Ala Ala Ile Val Asp Asn Arg Gly Ile Ile Gly Pro Gly Arg Arg
165 170 175
Ser Arg Ser Gly Pro Cys Leu Met Cys Val Asp Leu His Arg Cys Asp
180 185 190
Ile Asp Pro His Trp Leu Ser Ile Ile Asn Gln Gln Pro Asn Gly Pro
195 200 205
Thr Phe Pro Asp Pro Val Thr Glu Met Ala Thr Ala Ala Arg Leu Val
210 215 220
Ala Trp Val Thr Ala Asp Thr Trp Leu Pro Gly Val Val Glu Glu Val
225 230 235 240
Asn Pro His Asp Arg Thr Asn Ser Val Arg Thr Leu Pro Val His Pro
245 250 255
Lys Cys Pro Met Cys Trp Ser Leu gly Ser
260 265
<210>187
<211>1747
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1717)
<223>RXA0174 9
<400>187
ggtttttgcg tgctctggtt tagggactgg ttttgggaac gtgcccagtt ccacatcaaa 60
taacgctgag gtcgtactta atccatgaga tcatgaatgg gtg agc ttc ctt gta 115
Val Ser Phe Leu Val
1 5
gaa aat caa tta ctc gcg ttg gtt gtc atc atg acg gtc gga cta ttg 163
Glu Asn Gln Leu Leu Ala Leu Val Val Ile Met Thr Val Gly Leu Leu
10 15 20
ctc ggc cgc atc aaa att ttc ggg ttc cgt ctc ggc gtc gcc gct gta 211
Leu Gly Arg Ile Lys Ile Phe Gly Phe Arg Leu Gly Val Ala Ala Val
25 30 35
ctg ttt gta ggt cta gcg cta tcc acc att gag ccg gat att tcc gtc 259
Leu Phe Val Gly Leu Ala Leu Ser Thr Ile Glu Pro Asp Ile Ser Val
40 45 50
cca tcc ctc att tac gtg gtt gga ctg tcg ctt ttt gtc tac acg atc 307
Pro Ser Leu Ile Tyr Val Val Gly Leu Ser Leu Phe Val Tyr Thr Ile
55 60 65
ggt ctg gaa gcc ggc cct gga ttc ttc acc tcc atg aaa acc act ggt 355
Gly Leu Glu Ala Gly Pro Gly Phe Phe Thr Ser Met Lys Thr Thr Gly
70 75 80 85
ctg cgc aac aac gca ctg acc ttg ggc gcc atc atc gcc acc acg gca 403
Leu Arg Asn Asn Ala Leu Thr Leu Gly Ala Ile Ile Ala Thr Thr Ala
90 95 100
ctc gca tgg gca ctc atc aca gtt ttg aac atc gat gcc gcc tcc ggc 451
Leu Ala Trp Ala Leu Ile Thr Val Leu Asn Ile Asp Ala Ala Ser Gly
105 110 115
gcc ggc atg ctc acc ggc gcg ctc acc aac acc cca gcc atg gcc gca 499
Ala Gly Met Leu Thr Gly Ala Leu Thr Asn Thr Pro Ala Met Ala Ala
120 125 130
gtt gtt gac gca ctt cct tcg ctt atc gac gac acc ggc cag ctt cac 547
Val Val Asp Ala Leu Pro Ser Leu Ile Asp Asp Thr Gly Gln Leu His
135 140 145
ctc atc gcc gag ctg ccc gtc gtc gca tat tcc ttg gca tac ccc ctc 595
Leu Ile Ala Glu Leu Pro Val Val Ala Tyr Ser Leu Ala Tyr Pro Leu
150 155 160 165
ggt gtg ctc atc gtt att ctc tcc atc gcc atc ttc agc tct gtg ttc 643
Gly Val Leu Ile Val Ile Leu Ser Ile Ala Ile Phe Ser Ser Val Phe
170 175 180
aaa gtc gac cac aac aaa gaa gcc gaa gaa gcg ggc gtt gcg gtc cag 691
Lys Val Asp His Asn Lys Glu Ala Glu Glu Ala Gly Val Ala Val Gln
185 190 195
gaa ctc aaa ggc cgt cgc atc cgc gtc acc gtc gct gat ctt cca gcc 739
Glu Leu Lys Gly Arg Arg Ile Arg Val Thr Val Ala Asp Leu Pro Ala
200 205 210
ctg gag aac atc cca gag ctg ctc aac ctc cac gtc att gtg tcc cga 787
Leu Glu Asn Ile Pro Glu Leu Leu Asn Leu His Val Ile Val Ser Arg
215 220 225
gtg gaa cga gac ggt gag caa ttc atc ccg ctt tat ggc gaa cac gca 835
Val Glu Arg Asp Gly Glu Gln Phe Ile Pro Leu Tyr Gly Glu His Ala
230 235 240 245
cgc atc ggc gat gtc tta aca gtg gtg ggt gcc gat gaa gaa ctc aac 883
Arg Ile Gly Asp Val Leu Thr Val Val Gly Ala Asp Glu Glu Leu Asn
250 255 260
cgc gcg gaa aaa gcc atc ggt gaa ctc att gac ggc gac ccc tac agc 931
Arg Ala Glu Lys Ala Ile Gly Glu Leu Ile Asp Gly Asp Pro Tyr Ser
265 270 275
aat gtg gaa ctt gat tac cga cgc atc ttc gtc tca aac aca gca gtc 979
Asn Val Glu Leu Asp Tyr Arg Arg Ile Phe Val Ser Asn Thr Ala Val
280 285 290
gtg ggc act ccc cta tcc aag ctc cag cca ctg ttt aaa gac atg ctg 1027
Val Gly Thr Pro Leu Ser Lys Leu Gln Pro Leu Phe Lys Asp Met Leu
295 300 305
atc acc cgc atc agg cgc ggc gac aca gat ttg gtg gcc tcc tcc gac 1075
Ile Thr Arg Ile Arg Arg Gly Asp Thr Asp Leu Val Ala Ser Ser Asp
310 315 320 325
atg act ttg cag ctc ggt gac cgt gtc cgc gtt gtc gca cca gca gaa 1123
Met Thr Leu Gln Leu Gly Asp Arg Val Arg Val Val Ala Pro Ala Glu
330 335 340
aaa ctc cgc gaa gca acc caa ttg ctc ggc gat tcc tac aag aaa ctc 1171
Lys Leu Arg Glu Ala Thr Gln Leu Leu Gly Asp Ser Tyr Lys Lys Leu
345 350 355
tcc gat ttc aac ctg ctc cca ctc gct gcc ggc ctc atg atc ggt gtg 1219
Ser Asp Phe Asn Leu Leu Pro Leu Ala Ala Gly Leu Met Ile Gly Val
360 365 370
ctt gtc ggc atg gtg gag ttc cca cta cca ggt gga agc tcc ctg aaa 1267
Leu Val Gly Met Val Glu Phe Pro Leu Pro Gly Gly Ser Ser Leu Lys
375 380 385
ctg ggt aac gca ggt gga ccg cta gtt gtt gcg ctg ctg ctc ggc atg 1315
Leu Gly Asn Ala Gly Gly Pro Leu Val Val Ala Leu Leu Leu Gly Met
390 395 400 405
atc aat cgc aca ggc aag ttc gtc tgg caa atc ccc tac gga gca aac 1363
Ile Asn Arg Thr Gly Lys Phe Val Trp Gln Ile Pro Tyr Gly Ala Asn
410 415 420
ctt gcc ctt cgc caa ctg ggc atc aca cta ttt ttg gct gcc atc ggt 1411
Leu Ala Leu Arg Gln Leu Gly Ile Thr Leu Phe Leu Ala Ala Ile Gly
425 430 435
acc tca gcg ggc gca gga ttt cga tca gcg atc agc gac ccc caa tca 1459
Thr Ser Ala Gly Ala Gly Phe Arg Ser Ala Ile Ser Asp Pro Gln Ser
440 445 450
ctc acc atc atc ggc ttc ggt gcg ctg ctc act ttg ttc atc tcc atc 1507
Leu Thr Ile Ile Gly Phe Gly Ala Leu Leu Thr Leu Phe Ile Ser Ile
455 460 465
acg gtg ctg ttc gtt ggc cac aaa ctg atg aaa atc ccc ttc ggt gaa 1555
Thr Val Leu Phe Val Gly His Lys Leu Met Lys Ile Pro Phe Gly Glu
470 475 480 485
acc gct ggc atc ctc gcc ggt acg caa acc cac cct gct gtg ctg agt 1603
Thr Ala Gly Ile Leu Ala Gly Thr Gln Thr His Pro Ala Val Leu Ser
490 495 500
tat gtg tca gat gcc tcc cgc aac gag ctc cct gcc atg ggt tat acc 1651
Tyr Val Ser Asp Ala Ser Arg Asn Glu Leu Pro Ala Met Gly Tyr Thr
505 510 515
tct gtg tat ccg ctg gcg atg atc gca aag atc ctg gcc gcc caa acg 1699
Ser Val Tyr Pro Leu Ala Met Ile Ala Lys Ile Leu Ala Ala Gln Thr
520 525 530
ttg ttg ttc cta ctt atc tagcattgac cccttaagcg cagaaggcga 1747
Leu Leu Phe Leu Leu Ile
535
<210>188
<211>539
<212>PRT
<213>谷氨酸棒状杆菌
<400>188
Val Ser Phe Leu Val Glu Asn Gln Leu Leu Ala Leu Val Val Ile Met
1 5 10 15
Thr Val Gly Leu Leu Leu Gly Arg Ile Lys Ile Phe Gly Phe Arg Leu
20 25 30
Gly Val Ala Ala Val Leu Phe Val Gly Leu Ala Leu Ser Thr Ile Glu
35 40 45
Pro Asp Ile Ser Val Pro Ser Leu Ile Tyr Val Val Gly Leu Ser Leu
50 55 60
Phe Val Tyr Thr Ile Gly Leu Glu Ala Gly Pro Gly Phe Phe Thr Ser
65 70 75 80
Met Lys Thr Thr Gly Leu Arg Asn Asn Ala Leu Thr Leu Gly Ala Ile
85 90 95
Ile Ala Thr Thr Ala Leu Ala Trp Ala Leu Ile Thr Val Leu Asn Ile
100 105 110
Asp Ala Ala Ser Gly Ala Gly Met Leu Thr Gly Ala Leu Thr Asn Thr
115 120 125
Pro Ala Met Ala Ala Val Val Asp Ala Leu Pro Ser Leu Ile Asp Asp
130 135 140
Thr Gly Gln Leu His Leu Ile Ala Glu Leu Pro Val Val Ala Tyr Ser
145 150 155 160
Leu Ala Tyr Pro Leu Gly Val Leu Ile Val Ile Leu Ser Ile Ala Ile
165 170 175
Phe Ser Ser Val Phe Lys Val Asp His Asn Lys Glu Ala Glu Glu Ala
180 185 190
Gly Val Ala Val Gln Glu Leu Lys Gly Arg Arg Ile Arg Val Thr Val
195 200 205
Ala Asp Leu Pro Ala Leu Glu Asn Ile Pro Glu Leu Leu Asn Leu His
210 215 220
Val Ile Val Ser Arg Val Glu Arg Asp Gly Glu Gln Phe Ile Pro Leu
225 230 235 240
Tyr Gly Glu His Ala Arg Ile Gly Asp Val Leu Thr Val Val Gly Ala
245 250 255
Asp Glu Glu Leu Asn Arg Ala Glu Lys Ala Ile Gly Glu Leu Ile Asp
260 265 270
Gly Asp Pro Tyr Ser Asn Val Glu Leu Asp Tyr Arg Arg Ile Phe Val
275 280 285
Ser Asn Thr Ala Val Val Gly Thr Pro Leu Ser Lys Leu Gln Pro Leu
290 295 300
Phe Lys Asp Met Leu Ile Thr Arg Ile Arg Arg Gly Asp Thr Asp Leu
305 310 315 320
Val Ala Ser Ser Asp Met Thr Leu Gln Leu Gly Asp Arg Val Arg Val
325 330 335
Val Ala Pro Ala Glu Lys Leu Arg Glu Ala Thr Gln Leu Leu Gly Asp
340 345 350
Ser Tyr Lys Lys Leu Ser Asp Phe Asn Leu Leu Pro Leu Ala Ala Gly
355 360 365
Leu Met Ile Gly Val Leu Val Gly Met Val Glu Phe Pro Leu Pro Gly
370 375 380
Gly Ser Ser Leu Lys Leu Gly Asn Ala Gly Gly Pro Leu Val Val Ala
385 390 395 400
Leu Leu Leu Gly Met Ile Asn Arg Thr Gly Lys Phe Val Trp Gln Ile
405 410 415
Pro Tyr Gly Ala Asn Leu Ala Leu Arg Gln Leu Gly Ile Thr Leu Phe
420 425 430
Leu Ala Ala Ile Gly Thr Ser Ala Gly Ala Gly Phe Arg Ser Ala Ile
435 440 445
Ser Asp Pro Gln Ser Leu Thr Ile Ile Gly Phe Gly Ala Leu Leu Thr
450 455 460
Leu Phe Ile Ser Ile Thr Val Leu Phe Val Gly His Lys Leu Met Lys
465 470 475 480
Ile Pro Phe Gly Glu Thr Ala Gly Ile Leu Ala Gly Thr Gln Thr His
485 490 495
Pro Ala Val Leu Ser Tyr Val Ser Asp Ala Ser Arg Asn Glu Leu Pro
500 505 510
Ala Met Gly Tyr Thr Ser Val Tyr Pro Leu Ala Met Ile Ala Lys Ile
515 520 525
Leu Ala Ala Gln Thr Leu Leu Phe Leu Leu Ile
530 535
<210>189
<211>2071
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2041)
<223>RXA01754
<400>189
tgtggctgaa gacaagaatg aatagcattc atctcgtcga atttcatgta gaaatttgtc 60
cccctttttt ttgatgtgaa agttgaatcg gtaagctcct gtg aaa att aaa tcc 115
Val Lys Ile Lys Ser
1 5
gta ttt ttg agc acc gct tta agc gct tcc tta ctg ctc ggg atc acc 163
Val Phe Leu Ser Thr Ala Leu Ser Ala Ser Leu Leu Leu Gly Ile Thr
10 15 20
cca ccc gtg ctg gga gca acg atc aac ccc agt ttg cct ctt tct gcg 211
Pro Pro Val Leu Gly Ala Thr Ile Asn Pro Ser Leu Pro Leu Ser Ala
25 30 35
ttg agc tcc tcg gac gat atc gcc gta ccc aac ttc gcc aaa gaa tta 259
Leu Ser Ser Ser Asp Asp Ile Ala Val Pro Asn Phe Ala Lys Glu Leu
40 45 50
ccg tta gct ttt gat gta cca gca ggc act gtt ccc caa agc ttg agt 307
Pro Leu Ala Phe Asp Val Pro Ala Gly Thr Val Pro Gln Ser Leu Ser
55 60 65
gga acg ctg cag att cct gcc gag ttt tct ggc ggc gtc gtg gag ttt 355
Gly Thr Leu Gln Ile Pro Ala Glu Phe Ser Gly Gly Val Val Glu Phe
70 75 80 85
tat gac ggt gac cgg ctc ttt cac acc ctg cgc cta gaa gtt aat gat 403
Tyr Asp Gly Asp Arg Leu Phe His Thr Leu Arg Leu Glu Val Asn Asp
90 95 100
tcc cga gca cac att gag gtt ccg ctg caa agc gtt cct gtc gaa gac 451
Ser Arg Ala His Ile Glu Val Pro Leu Gln Ser Val Pro Val Glu Asp
105 110 115
ggc cgc gcc acc ttt tgg ttg cgc gcc atg ttg gat cct gta aac aac 499
Gly Arg Ala Thr Phe Trp Leu Arg Ala Met Leu Asp Pro Val Asn Asn
120 125 130
cag tgg tgc tac gag gag cag gaa gtc cgc ttc tta gac gga aac gtc 547
Gln Trp Cys Tyr Glu Glu Gln Glu Val Arg Phe Leu Asp Gly Asn Val
135 140 145
acc ttt gaa ggg gcg acg att aac cca gct gtg gtg gct gat tac ttc 595
Thr Phe Glu Gly Ala Thr Ile Asn Pro Ala Val Val Ala Asp Tyr Phe
150 155 160 165
ccg tca gtg ctg cgc gcg ttg aca att tac gtc ccg gaa aac ccc tct 643
Pro Ser Val Leu Arg Ala Leu Thr Ile Tyr Val Pro Glu Asn Pro Ser
170 175 180
gag gca gta caa gaa gcc acg tta gag gtt gcg acc tcc ctg gat tcg 691
Glu Ala Val Gln Glu Ala Thr Leu Glu Val Ala Thr Ser Leu Asp Ser
185 190 195
gtg tac cga aga tca ggt ctg gat gtc aac gta gaa acg ctt cca acc 739
Val Tyr Arg Arg Ser Gly Leu Asp Val Asn Val Glu Thr Leu Pro Thr
200 205 210
ggc acc gat gct cct cct aca cgt cct caa gat ttt gaa cgc cag att 787
Gly Thr Asp Ala Pro Pro Thr Arg Pro Gln Asp Phe Glu Arg Gln Ile
215 220 225
gtg ctg gtt gac gag gca aca gaa agt aac acg caa aaa acc gaa ttg 835
Val Leu Val Asp Glu Ala Thr Glu Ser Asn Thr Gln Lys Thr Glu Leu
230 235 240 245
gtc aat ccc ggc caa gac aat gca ttc ttg cgc ctg aac ggc aac gcc 883
Val Asn Pro Gly Gln Asp Asn Ala Phe Leu Arg Leu Asn Gly Asn Ala
250 255 260
gac gag ctt tac gat caa gcg cgc ttg ctt acc gac gca acc ctg cca 931
Asp Glu Leu Tyr Asp Gln Ala Arg Leu Leu Thr Asp Ala Thr Leu Pro
265 270 275
ctt gcc gta gac acc gaa gta acg gcc tca ggt ttt ggt gat gtg ccc 979
Leu Ala Val Asp Thr Glu Val Thr Ala Ser Gly Phe Gly Asp Val Pro
280 285 290
aac ctt tct aca gat gtg gcc acc ctc caa gaa ctg ggt atc acg cag 1027
Asn Leu Ser Thr Asp Val Ala Thr Leu Gln Glu Leu Gly Ile Thr Gln
295 300 305
ctc acc tct gaa tca gtt gcg cgc aca agc gtc acc ttg ggc att gaa 1075
Leu Thr Ser Glu Ser Val Ala Arg Thr Ser Val Thr Leu Gly Ile Glu
310 315 320 325
cgc tcc cgc ctg cgg acc tac tcg cag tcc atg gac ctg cac ata acg 1123
Arg Ser Arg Leu Arg Thr Tyr Ser Gln Ser Met Asp Leu His Ile Thr
330 335 340
gga acc tac acc cca ttg cca ccc caa aat gca gga cag atc acg ttc 1171
Gly Thr Tyr Thr Pro Leu Pro Pro Gln Asn Ala Gly Gln Ile Thr Phe
345 350 355
tcc att ggt gac acc gtg ttg gac tcc ttg acc acc gat gac act ggc 1219
Ser Ile Gly Asp Thr Val Leu Asp Ser Leu Thr Thr Asp Asp Thr Gly
360 365 370
atc att gac cgt gag ttc aac gtt cct gga gac ttg gtc aac cgc tac 1267
Ile Ile Asp Arg Glu Phe Asn Val Pro Gly Asp Leu Val Asn Arg Tyr
375 380 385
acg gcg atc gtc gtg gaa ttc acc agc acc ggc gac gtt aat tgt ggg 1315
Thr Ala Ile Val Val Glu Phe Thr Ser Thr Gly Asp Val Asn Cys Gly
390 395 400 405
gtc acc cag ccc gta ggc ctc aac att gat tcc gac agc ctt gtc acc 1363
Val Thr Gln Pro Val Gly Leu Asn Ile Asp Ser Asp Ser Leu Val Thr
410 415 420
tcc caa cat tca gat gtt cct gta ctc aac ggc ttc cgg tcc cta ccg 1411
Ser Gln His Ser Asp Val Pro Val Leu Asn Gly Phe Arg Ser Leu Pro
425 430 435
cag tcc ttc caa cct cgt gtg gac gtg gcg ttt gct gat ccc agc gtg 1459
Gln Ser Phe Gln Pro Arg Val Asp Val Ala Phe Ala Asp Pro Ser Val
440 445 450
cag gaa ctc tcc cgc gct gtc agc gta gtg ttg gga att caa tct atg 1507
Gln Glu Leu Ser Arg Ala Val Ser Val Val Leu Gly Ile Gln Ser Met
455 460 465
agc tcc cag cgc atc cgc cca cac ctg gtt aac tgg gat gaa gcc gta 1555
Ser Ser Gln Arg Ile Arg Pro His Leu Val Asn Trp Asp Glu Ala Val
470 475 480 485
gcc agc gag cgc cca aca att ttc att gat gct gcg ggc gca aag act 1603
Ala Ser Glu Arg Pro Thr Ile Phe Ile Asp Ala Ala Gly Ala Lys Thr
490 495 500
gat caa gtg cca agc tac ctc gcc caa caa ggc caa acc cta gag atc 1651
Asp Gln Val Pro Ser Tyr Leu Ala Gln Gln Gly Gln Thr Leu Glu Ile
505 510 515
acc agc aag aac gac caa aat gcg gac ggc gaa caa ctc acc cga tcc 1699
Thr Ser Lys Asn Asp Gln Asn Ala Asp Gly Glu Gln Leu Thr Arg Ser
520 525 530
ctg caa acc aac gct gcg ctt gtt gtc ggt tcc att cag gcc gtg tgg 1747
Leu Gln Thr Asn Ala Ala Leu Val Val Gly Ser Ile Gln Ala Val Trp
535 540 545
gat gcc gat aag aag cgc acg gtg att gtg gca agt tcc cag gac aac 1795
Asp Ala Asp Lys Lys Arg Thr Val Ile Val Ala Ser Ser Gln Asp Asn
550 555 560 565
ccc gcc gat ttg gat gcc ttg att tcg tgg atg gga gaa gac cgc gaa 1843
Pro Ala Asp Leu Asp Ala Leu Ile Ser Trp Met Gly Glu Asp Arg Glu
570 575 580
cgc tgg agt gat ctc aac ggc gac ctg att gtc aaa gtc cga gac cgc 1891
Arg Trp Ser Asp Leu Asn Gly Asp Leu Ile Val Lys Val Arg Asp Arg
585 590 595
gaa cct gtg caa ttg acc acc gtg gaa gcc cca gat cag cct ggt cga 1939
Glu Pro Val Gln Leu Thr Thr Val Glu Ala Pro Asp Gln Pro Gly Arg
600 605 610
tcg gcc aca gcc ttt att gcg atc ggc gtc agc ctt gtg gtc att gcc 1987
Ser Ala Thr Ala Phe Ile Ala Ile Gly Val Ser Leu Val Val Ile Ala
615 620 625
ctg att gtc gca gcc gtg gtg tca gtg tcc agg cgt tcg caa aaa gga 2035
Leu Ile Val Ala Ala Val Val Ser Val Ser Arg Arg Ser Gln Lys Gly
630 635 640 645
tac aaa tgatttcttc tttacccagt agaacgcagg 2071
Tyr Lys
<210>190
<211>647
<212>PRT
<213>谷氨酸棒状杆菌
<400>190
Val Lys Ile Lys Ser Val Phe Leu Ser Thr Ala Leu Ser Ala Ser Leu
1 5 10 15
Leu Leu Gly Ile Thr Pro Pro Val Leu Gly Ala Thr Ile Asn Pro Ser
20 25 30
Leu Pro Leu Ser Ala Leu Ser Ser Ser Asp Asp Ile Ala Val Pro Asn
35 40 45
Phe Ala Lys Glu Leu Pro Leu Ala Phe Asp Val Pro Ala Gly Thr Val
50 55 60
Pro Gln Ser Leu Ser Gly Thr Leu Gln Ile Pro Ala Glu Phe Ser Gly
65 70 75 80
Gly Val Val Glu Phe Tyr Asp Gly Asp Arg Leu Phe His Thr Leu Arg
85 90 95
Leu Glu Val Asn Asp Ser Arg Ala His Ile Glu Val Pro Leu Gln Ser
100 105 110
Val Pro Val Glu Asp Gly Arg Ala Thr Phe Trp Leu Arg Ala Met Leu
115 120 125
Asp Pro Val Asn Asn Gln Trp Cys Tyr Glu Glu Gln Glu Val Arg Phe
130 135 140
Leu Asp Gly Asn Val Thr Phe Glu Gly Ala Thr Ile Asn Pro Ala Val
145 150 155 160
Val Ala Asp Tyr Phe Pro Ser Val Leu Arg Ala Leu Thr Ile Tyr Val
165 170 175
Pro Glu Asn Pro Ser Glu Ala Val Gln Glu Ala Thr Leu Glu Val Ala
180 185 190
Thr Ser Leu Asp Ser Val Tyr Arg Arg Ser Gly Leu Asp Val Asn Val
195 200 205
Glu Thr Leu Pro Thr Gly Thr Asp Ala Pro Pro Thr Arg Pro Gln Asp
210 215 220
Phe Glu Arg Gln Ile Val Leu Val Asp Glu Ala Thr Glu Ser Asn Thr
225 230 235 240
Gln Lys Thr Glu Leu Val Asn Pro Gly Gln Asp Asn Ala Phe Leu Arg
245 250 255
Leu Asn Gly Asn Ala Asp Glu Leu Tyr Asp Gln Ala Arg Leu Leu Thr
260 265 270
Asp Ala Thr Leu Pro Leu Ala Val Asp Thr Glu Val Thr Ala Ser Gly
275 280 285
Phe Gly Asp Val Pro Asn Leu Ser Thr Asp Val Ala Thr Leu Gln Glu
290 295 300
Leu Gly Ile Thr Gln Leu Thr Ser Glu Ser Val Ala Arg Thr Ser Val
305 310 315 320
Thr Leu Gly Ile Glu Arg Ser Arg Leu Arg Thr Tyr Ser Gln Ser Met
325 330 335
Asp Leu His Ile Thr Gly Thr Tyr Thr Pro Leu Pro Pro Gln Asn Ala
340 345 350
Gly Gln Ile Thr Phe Ser Ile Gly Asp Thr Val Leu Asp Ser Leu Thr
355 360 365
Thr Asp Asp Thr Gly Ile Ile Asp Arg Glu Phe Asn Val Pro Gly Asp
370 375 380
Leu Val Asn Arg Tyr Thr Ala Ile Val Val Glu Phe Thr Ser Thr Gly
385 390 395 400
Asp Val Asn Cys Gly Val Thr Gln Pro Val Gly Leu Asn Ile Asp Ser
405 410 415
Asp Ser Leu Val Thr Ser Gln His Ser Asp Val Pro Val Leu Asn Gly
420 425 430
Phe Arg Ser Leu Pro Gln Ser Phe Gln Pro Arg Val Asp Val Ala Phe
435 440 445
Ala Asp Pro Ser Val Gln Glu Leu Ser Arg Ala Val Ser Val Val Leu
450 455 460
Gly Ile Gln Ser Met Ser Ser Gln Arg Ile Arg Pro His Leu Val Asn
465 470 475 480
Trp Asp Glu Ala Val Ala Ser Glu Arg Pro Thr Ile Phe Ile Asp Ala
485 490 495
Ala Gly Ala Lys Thr Asp Gln Val Pro Ser Tyr Leu Ala Gln Gln Gly
500 505 510
Gln Thr Leu Glu Ile Thr Ser Lys Asn Asp Gln Asn Ala Asp Gly Glu
515 520 525
Gln Leu Thr Arg Ser Leu Gln Thr Asn Ala Ala Leu Val Val Gly Ser
530 535 540
Ile Gln Ala Val Trp Asp Ala Asp Lys Lys Arg Thr Val Ile Val Ala
545 550 555 560
Ser Ser Gln Asp Asn Pro Ala Asp Leu Asp Ala Leu Ile Ser Trp Met
565 570 575
Gly Glu Asp Arg Glu Arg Trp Ser Asp Leu Asn Gly Asp Leu Ile Val
580 585 590
Lys Val Arg Asp Arg Glu Pro Val Gln Leu Thr Thr Val Glu Ala Pro
595 600 605
Asp Gln Pro Gly Arg Ser Ala Thr Ala Phe Ile Ala Ile Gly Val Ser
610 615 620
Leu Val Val Ile Ala Leu Ile Val Ala Ala Val Val Ser Val Ser Arg
625 630 635 640
Arg Ser Gln Lys Gly Tyr Lys
645
<210>191
<211>4594
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(4564)
<223>RXA01761
<400>191
ttaaagtttc tatattccat tctaaaataa ctttgaattg gggattaatg aaacttttta 60
agcaattgtt taaaacgaat attgaggggg tggcgggcaa gtg act gcg aca gtt 115
Val Thr Ala Thr Val
1 5
tta gaa ccg caa cct gta cag ctt agt gag cag gac gta gat gct gag 163
Leu Glu Pro Gln Pro Val Gln Leu Ser Glu Gln Asp Val Asp Ala Glu
10 15 20
att gcg cgc atg gag ctt aaa cac aac cga cac aag acg tgg cga cgc 211
Ile Ala Arg Met Glu Leu Lys His Asn Arg His Lys Thr Trp Arg Arg
25 30 35
cga acg atg gct gtt gtc atg acg ctc ttg atc tca gtg ggt ttg agc 259
Arg Thr Met Ala Val Val Met Thr Leu Leu Ile Ser Val Gly Leu Ser
40 45 50
ttt ggt gcg ttt ggt aac aag gaa cgt gaa gct aat gct ttc gcc agc 307
Phe Gly Ala Phe Gly Asn Lys Glu Arg Glu Ala Asn Ala Phe Ala Ser
55 60 65
gct att att gcc cag gtt gtc ggt gct atg ggc gag gtt gcc ttt gaa 355
Ala Ile Ile Ala Gln Val Val Gly Ala Met Gly Glu Val Ala Phe Glu
70 75 80 85
gcg att tgt ccc agt gat ggt gac acc gag atg ctt ctt aag tgc atc 403
Ala Ile Cys Pro Ser Asp Gly Asp Thr Glu Met Leu Leu Lys Cys Ile
90 95 100
acc gag aac tta ggt gaa atg cac att atc gag aag tgt ctc gaa gca 451
Thr Glu Asn Leu Gly Glu Met His Ile Ile Glu Lys Cys Leu Glu Ala
105 110 115
gaa gat gtc ttg aag tgc ttc tac gat gcg aaa aac gaa gag caa cgt 499
Glu Asp Val Leu Lys Cys Phe Tyr Asp Ala Lys Asn Glu Glu Gln Arg
120 125 130
aaa gaa cag aac ctt gat aaa gcc cct gat tac tcc atg tac cgt atg 547
Lys Glu Gln Asn Leu Asp Lys Ala Pro Asp Tyr Ser Met Tyr Arg Met
135 140 145
gcc tct gcg atg gca tcg ttc tat ggc aat ggt cgg gca gct act gcc 595
Ala Ser Ala Met Ala Ser Phe Tyr Gly Asn Gly Arg Ala Ala Thr Ala
150 155 160 165
ggt gta gag gaa ggc gga ccg aac gag ttc ctc gat tct gag gac gcc 643
Gly Val Glu Glu Gly Gly Pro Asn Glu Phe Leu Asp Ser Glu Asp Ala
170 175 180
ggt ctt aag gtg tgg gag ggc att ctc agt aag gcc gcc aac gga ggc 691
Gly Leu Lys Val Trp Glu Gly Ile Leu Ser Lys Ala Ala Asn Gly Gly
185 190 195
aat gtc ctt ggt tat gct gac gcg aaa cac aac gag gac tct ggt tgg 739
Asn Val Leu Gly Tyr Ala Asp Ala Lys His Asn Glu Asp Ser Gly Trp
200 205 210
ttc ttt ggt aat ggt gtt gcc aac aac gag aaa acg tac tcc tat gat 787
Phe Phe Gly Asn Gly Val Ala Asn Asn Glu Lys Thr Tyr Ser Tyr Asp
215 220 225
tct cta gct gat cat gcc ttt caa ggg cct tat cac ttt gca tta ttt 835
Ser Leu Ala Asp His Ala Phe Gln Gly Pro Tyr His Phe Ala Leu Phe
230 235 240 245
ggc gcg aca ctg agt ggc tta ggc ttt gat agc tca aag gcg gag gat 883
Gly Ala Thr Leu Ser Gly Leu Gly Phe Asp Ser Ser Lys Ala Glu Asp
250 255 260
tcg cag act gat ttt gct cag cgt aag ggc atg ggg tac gcg atg atg 931
Ser Gln Thr Asp Phe Ala Gln Arg Lys Gly Met Gly Tyr Ala Met Met
265 270 275
atc gcg tac atc atc tcc gga ggc att gat ctg gtt ttt aac act gtg 979
Ile Ala Tyr Ile Ile Ser Gly Gly Ile Asp Leu Val Phe Asn Thr Val
280 285 290
ctg aat atc ttg agc acc ata aac cca ttt aga ctg ctc gtt gga cca 1027
Leu Asn Ile Leu Ser Thr Ile Asn Pro Phe Arg Leu Leu Val Gly Pro
295 300 305
gta tct cag aat act aac aag aca ttc act gag aat atg gct ggt ggg 1075
Val Ser Gln Asn Thr Asn Lys Thr Phe Thr Glu Asn Met Ala Gly Gly
310 315 320 325
cag acc aca gaa gga aca ccg ttt gaa gga atg act gat ttc ttc ggg 1123
Gln Thr Thr Glu Gly Thr Pro Phe Glu Gly Met Thr Asp Phe Phe Gly
330 335 340
ttg atc tat aac tgg tca gtg act gtg ggc tgg ctc atc gtt att cct 1171
Leu Ile Tyr Asn Trp Ser Val Thr Val Gly Trp Leu Ile Val Ile Pro
345 350 355
gtc tcc att ggt ctg ttt atg atg ggt gcg ttg atg ttt cga cgc atg 1219
Val Ser Ile Gly Leu Phe Net Met Gly Ala Leu Met Phe Arg Arg Met
360 365 370
gac aag gga tca gcg ttt aaa aag gtt gtt atc cgc gtc gtg tac ggt 1267
Asp Lys Gly Ser Ala Phe Lys Lys Val Val Ile Arg Val Val Tyr Gly
375 380 385
gtc gtt ggc ctg cca ctg ctt ggt gtc tct tat acc ggt gcg ctg gat 1315
Val Val Gly Leu Pro Leu Leu Gly Val Ser Tyr Thr Gly Ala Leu Asp
390 395 400 405
tcc ttc ctc gac agc ggt agt tcc aca agt gct gga tca aat gcg aca 1363
Ser Phe Leu Asp Ser Gly Ser Ser Thr Ser Ala Gly Ser Asn Ala Thr
410 415 420
aag att gtg ttg tcc acc tac gtg gat ttc caa tca tgg gca gaa aac 1411
Lys Ile Val Leu Ser Thr Tyr Val Asp Phe Gln Ser Trp Ala Glu Asn
425 430 435
acc cgt atg cgt gtt cct gac aaa gta aca ctg gcg tgg agt ctg gag 1459
Thr Arg Met Arg Val Pro Asp Lys Val Thr Leu Ala Trp Ser Leu Glu
440 445 450
gat cag gcg cca aca ggg cag tcc atg cgg atg gta cgc aat tcg gcg 1507
Asp Gln Ala Pro Thr Gly Gln Ser Met Arg Met Val Arg Asn Ser Ala
455 460 465
ttg gaa att aat gct caa tca aat gag agt ttt act act ttt aag aat 1555
Leu Glu Ile Asn Ala Gln Ser Asn Glu Ser Phe Thr Thr Phe Lys Asn
470 475 480 485
cca ggt tcc atg gat cta ggc tat agc atg gac tgg atg gaa aag atg 1603
Pro Gly Ser Met Asp Leu Gly Tyr Ser Met Asp Trp Met Glu Lys Met
490 495 500
gcg gat cca gcc aat tct ggc acc gat ggg acc tca tca agt tcg gcg 1651
Ala Asp Pro Ala Asn Ser Gly Thr Asp Gly Thr Ser Ser Ser Ser Ala
505 510 515
acg act att ttc cag gga acc atc gcg cta ctg agt cgt tat atc gac 1699
Thr Thr Ile Phe Gln Gly Thr Ile Ala Leu Leu Ser Arg Tyr Ile Asp
520 525 530
aac gac agt atc tcc tct ggc agt ttc gaa acc tct att cga agt gcg 1747
Asn Asp Ser Ile Ser Ser Gly Ser Phe Glu Thr Ser Ile Arg Ser Ala
535 540 545
atg gaa tct ttg acc tcg aaa gag gga agt ggc gaa gct gtt gcc ggt 1795
Met Glu Ser Leu Thr Ser Lys Glu Gly Ser Gly Glu Ala Val Ala Gly
550 555 560 565
ggt gat gta atg gcc tgg gtt act ggt tac aac aca gcc tct ggt ctt 1843
Gly Asp Val Met Ala Trp Val Thr Gly Tyr Asn Thr Ala Ser Gly Leu
570 575 580
aat gag atc act gct gct gaa tta gct gat gcg aac aat cca ttg ctt 1891
Asn Glu Ile Thr Ala Ala Glu Leu Ala Asp Ala Asn Asn Pro Leu Leu
585 590 595
gag gtc agc gac tca gct ggt ctg cag gga cga cct att gac cgc aat 1939
Glu Val Ser Asp Ser Ala Gly Leu Gln Gly Arg Pro Ile Asp Arg Asn
600 605 610
gcg gtg tca ttt aaa tct ggt gat gcg aca gcg tca tgc aac aac tca 1987
Ala Val Ser Phe Lys Ser Gly Asp Ala Thr Ala Ser Cys Asn Asn Ser
615 620 625
gtc gtg aca ggt tct cgt gct gtg gtc ggg gac tac cga agt gac tgc 2035
Val Val Thr Gly Ser Arg Ala Val Val Gly Asp Tyr Arg Ser Asp Cys
630 635 640 645
aat atg tcg ccc ttg acg atg tac aac ttc ctc aac acc tca ttc cat 2083
Asn Met Ser Pro Leu Thr Met Tyr Asn Phe Leu Asn Thr Ser Phe His
650 655 660
cca gct gat gca agt gtc ttt tct acg tcc act tct gcg tca agc tac 2131
Pro Ala Asp Ala Ser Val Phe Ser Thr Ser Thr Ser Ala Ser Ser Tyr
665 670 675
acc cgt gca tcg cat agc gca gtg agt ctt atc ggt tct ggt gcc atg 2179
Thr Arg Ala Ser His Ser Ala Val Ser Leu Ile Gly Ser Gly Ala Met
680 685 690
aac ttt gtg tac tgg ttc tct gca atg tcc ctg atg ggt agc ttc atc 2227
Asn Phe Val Tyr Trp Phe Ser Ala Met Ser Leu Met Gly Ser Phe Ile
695 700 705
gtg atc ggt atc ggt tac gcc ggt gcc atg ctg ttt aat acg att cga 2275
Val Ile Gly Ile Gly Tyr Ala Gly Ala Met Leu Phe Asn Thr Ile Arg
710 715 720 725
cgc acg ctg tcg ctc att ggt gcc gtt cct ttt gct gcg atg ggc ttt 2323
Arg Thr Leu Ser Leu Ile Gly Ala Val Pro Phe Ala Ala Met Gly Phe
730 735 740
att gct ggt gtt gcc aag gtc att gtg tac acc atc gct atg ctc gta 2371
Ile Ala Gly Val Ala Lys Val Ile Val Tyr Thr Ile Ala Met Leu Val
745 750 755
gag gtt atc gga acg atc att ttg tac cag ctc att acc cgg ttc ttg 2419
Glu Val Ile Gly Thr Ile Ile Leu Tyr Gln Leu Ile Thr Arg Phe Leu
760 765 770
atg gct gta cca gca ctc ttt gaa caa cct ttg gct gat tcc tta agt 2467
Met Ala Val Pro Ala Leu Phe Glu Gln Pro Leu Ala Asp Ser Leu Ser
775 780 785
agt aac gag agt gct gag ctg gtt gcc ggt gct ggt atg ggg cta gtc 2515
Ser Asn Glu Ser Ala Glu Leu Val Ala Gly Ala Gly Met Gly Leu Val
790 795 800 805
ggt atg gca cta gct acc tca ggc aac tgg gcg att gcc ggc atg gtg 2563
Gly Met Ala Leu Ala Thr Ser Gly Asn Trp Ala Ile Ala Gly Met Val
810 815 820
atc aca gtg gcc tca tcc atg ggg gtc atc atc ttc acg att att gcg 2611
Ile Thr Val Ala Ser Ser Met Gly Val Ile Ile Phe Thr Ile Ile Ala
825 830 835
atg aag gtg cgc gga tct ttg gta tca ggt gtc gat gaa gcc gtg acc 2659
Met Lys Val Arg Gly Ser Leu Val Ser Gly Val Asp Glu Ala Val Thr
840 845 850
agt gtt att aac cgc ttc ttg gat aca cag gtc tct tct gca ggt gct 2707
Ser Val Ile Asn Arg Phe Leu Asp Thr Gln Val Ser Ser Ala Gly Ala
855 860 865
acc tct ggt gac ggc atg atg cgt cgt gca gcc gca acg ggt ctt ggt 2755
Thr Ser Gly Asp Gly Met Met Arg Arg Ala Ala Ala Thr Gly Leu Gly
870 875 880 885
att ggt gcg aca cat atg gta ctc aac cgt gat ggt gac ggt ggt gga 2803
Ile Gly Ala Thr His Met Val Leu Asn Arg Asp Gly Asp Gly Gly Gly
890 895 900
tct gat tca ggc tct ggt gga tca ggt ggc ggt agc gat tca ggc ttg 2851
Ser Asp Ser Gly Ser Gly Gly Ser Gly Gly Gly Ser Asp Ser Gly Leu
905 910 915
ggt gag aag gct gct ggt ctg gcg aag gtt gtc aca act gtg gct ggt 2899
Gly Glu Lys Ala Ala Gly Leu Ala Lys Val Val Thr Thr Val Ala Gly
920 925 930
gcc gga tta gtc ggt aag tat gct acg gat gcg ctc gat aat tat gca 2947
Ala Gly Leu Val Gly Lys Tyr Ala Thr Asp Ala Leu Asp Asn Tyr Ala
935 940 945
gat ggc gtt atc aac ggc gac ggt gat ggt gcg ttc gca gca ggt ggt 2995
Asp Gly Val Ile Asn Gly Asp Gly Asp Gly Ala Phe Ala Ala Gly Gly
950 955 960 965
gac gct acc gtt gat ggc gac tat gta gca gat gga gat gca att gct 3043
Asp Ala Thr Val Asp Gly Asp Tyr Val Ala Asp Gly Asp Ala Ile Ala
970 975 980
tct gct gat gct aac gct gat ttt gta gac ggt gtt gtt gat ggt gct 3091
Ser Ala Asp Ala Asn Ala Asp Phe Val Asp Gly Val Val Asp Gly Ala
985 990 995
ggt cgc gcg tca ttt agc aat gct gcg tat agc tcc gat gga acc act 3139
Gly Arg Ala Ser Phe Ser Asn Ala Ala Tyr Ser Ser Asp Gly Thr Thr
1000 1005 1010
ctc gac ggt gag ggt gcg agc gtt gat gca cag ggt aac ccg ctt cat 3187
Leu Asp Gly Glu Gly Ala Ser Val Asp Ala Gln Gly Asn Pro Leu His
1015 1020 1025
gct gat ggc aca cca atg agt gct gct gaa gct gaa atg aag atg gct 3235
Ala Asp Gly Thr Pro Met Ser Ala Ala Glu Ala Glu Met Lys Met Ala
1030 1035 1040 1045
ggt ctg agc tcg tca gga acc atg atg gag aaa tct ggt gtg aaa tcg 3283
Gly Leu Ser Ser Ser Gly Thr Met Met Glu Lys Ser Gly Val Lys Ser
1050 1055 1060
agt ggc att acc act gca gcg gat gtc atg gac gat cag tct ctg gca 3331
Ser Gly Ile Thr Thr Ala Ala Asp Val Met Asp Asp Gln Ser Leu Ala
1065 1070 1075
agc agt gtc act gag tct ggt ctg tcc aag att cca gac acc tat ggt 3379
Ser Ser Val Thr Glu Ser Gly Leu Ser Lys Ile Pro Asp Thr Tyr Gly
1080 1085 1090
gca gat gtc tcg ggt gct gcg ggc aca gtc gga act acc ggt gct gat 3427
Ala Asp Val Ser Gly Ala Ala Gly Thr Val Gly Thr Thr Gly Ala Asp
1095 1100 1105
tac agt gcg acc gat tca agc gca ggt ctg aac atg agc gag gct gca 3475
Tyr Ser Ala Thr Asp Ser Ser Ala Gly Leu Asn Met Ser Glu Ala Ala
1110 1115 1120 1125
ttg cag agt ggc acc cca atg ggc gct ctc gct ggt gga tct gtg tcg 3523
Leu Gln Ser Gly Thr Pro Met Gly Ala Leu Ala Gly Gly Ser Val Ser
1130 1135 1140
agt tcc gat cag gcc atg aat gac gca gct ctt cag att gca gcg tct 3571
Ser Ser Asp Gln Ala Met Asn Asp Ala Ala Leu Gln Ile Ala Ala Ser
1145 1150 1155
cag ggt ctt gca cca gca ggt tcc ata gct ggt atg gag caa ctt agt 3619
Gln Gly Leu Ala Pro Ala Gly Ser Ile Ala Gly Met Glu Gln Leu Ser
1160 1165 1170
gct caa gcc act gaa gca cct gct gga aag gcc ggc aag cag ctt ggc 3667
Ala Gln Ala Thr Glu Ala Pro Ala Gly Lys Ala Gly Lys Gln Leu Gly
1175 1180 1185
gat ctt tct ggc tca gcg ctc aat act cag ctg gcg tcc atg gga cag 3715
Asp Leu Ser Gly Ser Ala Leu Asn Thr Gln Leu Ala Ser Met Gly Gln
1190 1195 1200 1205
cag gta ggt gac agt gtg aac agc gct tat gct gca ggc ggt atg ggt 3763
Gln Val Gly Asp Ser Val Asn Ser Ala Tyr Ala Ala Gly Gly Met Gly
1210 1215 1220
ggt gtt gat gtg gct ggc aag gtc acc gag gca gca cag cac ttg tct 3811
Gly Val Asp Val Ala Gly Lys Val Thr Glu Ala Ala Gln His Leu Ser
1225 1230 1235
cag gtt cca ggt cag att cag aat gct gtg acc aat gcg gat gct ggt 3859
Gln Val Pro Gly Gln Ile Gln Asn Ala Val Thr Asn Ala Asp Ala Gly
1240 1245 1250
tcc tct ggc gca agc ttt ggt cag atg gca cag ggg gca gct ggt att 3907
Ser Ser Gly Ala Ser Phe Gly Gln Met Ala Gln Gly Ala Ala Gly Ile
1255 1260 1265
gcc ggt gtc gca ggt gtg atc ggt gca gcg ggc gca gca agc tct gca 3955
Ala Gly Val Ala Gly Val Ile Gly Ala Ala Gly Ala Ala Ser Ser Ala
1270 1275 1280 1285
gca caa ggc gca ggt act gtc cag ggt gcg atg ggt aat gct gca gct 4003
Ala Gln Gly Ala Gly Thr Val Gln Gly Ala Met Gly Asn Ala Ala Ala
1290 1295 1300
ggt gcg gga atg atc aac aac gct gtt tcc ggt gga gct act ggc tca 4051
Gly Ala Gly Met Ile Asn Asn Ala Val Ser Gly Gly Ala Thr Gly Ser
1305 1310 1315
aca ggt gcc gca cat gtg gtc aat gca tca cat gga cca gtg gcg cct 4099
Thr Gly Ala Ala His Val Val Asn Ala Ser His Gly Pro Val Ala Pro
1320 1325 1330
ggt cag gct cac tac caa gag tct ggt cat gca caa gca ttt gtg cag 4147
Gly Gln Ala His Tyr Gln Glu Ser Gly His Ala Gln Ala Phe Val Gln
1335 1340 1345
aac aac cag gcc aac acc gcg cac aca gca aac acg cgt gca ccg tca 4195
Asn Asn Gln Ala Asn Thr Ala His Thr Ala Asn Thr Arg Ala Pro Ser
1350 1355 1360 1365
tca gct caa att atg ggc gcg aac gtt gct ggc tca ctg gca tca cag 4243
Ser Ala Gln Ile Met Gly Ala Asn Val Ala Gly Ser Leu Ala Ser Gln
1370 1375 1380
gct gta cga gga atc ggt cag cct ggt cag atg ggt gct aat gtt cgc 4291
Ala Val Arg Gly Ile Gly Gln Pro Gly Gln Met Gly Ala Asn Val Arg
1385 1390 1395
gac gcg atg ggt ggc agc gga cgc tct ggt ggc cgt ggt gga gca act 4339
Asp Ala Met Gly Gly Ser Gly Arg Ser Gly Gly Arg Gly Gly Ala Thr
1400 1405 1410
caa ggc ggt cga ggc gca cag cgc agc ggt gtc agt gct aag aac ggt 4387
Gln Gly Gly Arg Gly Ala Gln Arg Ser Gly Val Ser Ala Lys Asn Gly
1415 1420 1425
atc cgt gca cag cga ggt cag aag cct tct gtg acc ggc cag gcg atg 4435
Ile Arg Ala Gln Arg Gly Gln Lys Pro Ser Val Thr Gly Gln Ala Met
1430 1435 1440 1445
aat gca gca atg cgt tca gca gcg gta agc ggt cgc atg gca aac atg 4483
Asn Ala Ala Met Arg Ser Ala Ala Val Ser Gly Arg Met Ala Asn Met
1450 1455 1460
gac ggc aac agt gta ggt ggc act gaa gca gat cca cag cag ggg agt 4531
Asp Gly Asn Ser Val Gly Gly Thr Glu Ala Asp Pro Gln Gln Gly Ser
1465 1470 1475
ggc gta acc gag aag ggt gat aaa ggc gtt aaa tagcgtgtaa aacacaatga 4584
Gly Val Thr Glu Lys Gly Asp Lys Gly Val Lys
1480 1485
ccgtggagag 4594
<210>192
<211>1488
<212>PRT
<213>谷氨酸棒状杆菌
<400>192
Val Thr Ala Thr Val Leu Glu Pro Gln Pro Val Gln Leu Ser Glu Gln
1 5 10 15
Asp Val Asp Ala Glu Ile Ala Arg Met Glu Leu Lys His Asn Arg His
20 25 30
Lys Thr Trp Arg Arg Arg Thr Met Ala Val Val Met Thr Leu Leu Ile
35 40 45
Ser Val Gly Leu Ser Phe Gly Ala Phe Gly Asn Lys Glu Arg Glu Ala
50 55 60
Asn Ala Phe Ala Ser Ala Ile Ile Ala Gln Val Val Gly Ala Met Gly
65 70 75 80
Glu Val Ala Phe Glu Ala Ile Cys Pro Ser Asp Gly Asp Thr Glu Met
85 90 95
Leu Leu Lys Cys Ile Thr Glu Asn Leu Gly Glu Met His Ile Ile Glu
100 105 110
Lys Cys Leu Glu Ala Glu Asp Val Leu Lys Cys Phe Tyr Asp Ala Lys
115 120 125
Asn Glu Glu Gln Arg Lys Glu Gln Asn Leu Asp Lys Ala Pro Asp Tyr
130 135 140
Ser Met Tyr Arg Met Ala Ser Ala Met Ala Ser Phe Tyr Gly Asn Gly
145 150 155 160
Arg Ala Ala Thr Ala Gly Val Glu Glu Gly Gly Pro Asn Glu Phe Leu
165 170 175
Asp Ser Glu Asp Ala Gly Leu Lys Val Trp Glu Gly Ile Leu Ser Lys
180 185 190
Ala Ala Asn Gly Gly Asn Val Leu Gly Tyr Ala Asp Ala Lys His Asn
195 200 205
Glu Asp Ser Gly Trp Phe Phe Gly Asn Gly Val Ala Asn Asn Glu Lys
210 215 220
Thr Tyr Ser Tyr Asp Ser Leu Ala Asp His Ala Phe Gln Gly Pro Tyr
225 230 235 240
His Phe Ala Leu Phe Gly Ala Thr Leu Ser Gly Leu Gly Phe Asp Ser
245 250 255
Ser Lys Ala Glu Asp Ser Gln Thr Asp Phe Ala Gln Arg Lys Gly Met
260 265 270
Gly Tyr Ala Met Met Ile Ala Tyr Ile Ile Ser Gly Gly Ile Asp Leu
275 280 285
Val Phe Asn Thr Val Leu Asn Ile Leu Ser Thr Ile Asn Pro Phe Arg
290 295 300
Leu Leu Val Gly Pro Val Ser Gln Asn Thr Asn Lys Thr Phe Thr Glu
305 310 315 320
Asn Met Ala Gly Gly Gln Thr Thr Glu Gly Thr Pro Phe Glu Gly Met
325 330 335
Thr Asp Phe Phe Gly Leu Ile Tyr Asn Trp Ser Val Thr Val Gly Trp
340 345 350
Leu Ile Val Ile Pro Val Ser Ile Gly Leu Phe Met Met Gly Ala Leu
355 360 365
Met Phe Arg Arg Met Asp Lys Gly Ser Ala Phe Lys Lys Val Val Ile
370 375 380
Arg Val Val Tyr Gly Val Val Gly Leu Pro Leu Leu Gly Val Ser Tyr
385 390 395 400
Thr Gly Ala Leu Asp Ser Phe Leu Asp Ser Gly Ser Ser Thr Ser Ala
405 410 415
Gly Ser Asn Ala Thr Lys Ile Val Leu Ser Thr Tyr Val Asp Phe Gln
420 425 430
Ser Trp Ala Glu Asn Thr Arg Met Arg Val Pro Asp Lys Val Thr Leu
435 440 445
Ala Trp Ser Leu Glu Asp Gln Ala Pro Thr Gly Gln Ser Met Arg Met
450 455 460
Val Arg Asn Ser Ala Leu Glu Ile Asn Ala Gln Ser Asn Glu Ser Phe
465 470 475 480
Thr Thr Phe Lys Asn Pro Gly Ser Met Asp Leu Gly Tyr Ser Met Asp
485 490 495
Trp Met Glu Lys Met Ala Asp Pro Ala Asn Ser Gly Thr Asp Gly Thr
500 505 510
Ser Ser Ser Ser Ala Thr Thr Ile Phe Gln Gly Thr Ile Ala Leu Leu
515 520 525
Ser Arg Tyr Ile Asp Asn Asp Ser Ile Ser Ser Gly Ser Phe Glu Thr
530 535 540
Ser Ile Arg Ser Ala Met Glu Ser Leu Thr Ser Lys Glu Gly Ser Gly
545 550 555 560
Glu Ala Val Ala Gly Gly Asp Val Met Ala Trp Val Thr Gly Tyr Asn
565 570 575
Thr Ala Ser Gly Leu Asn Glu Ile Thr Ala Ala Glu Leu Ala Asp Ala
580 585 590
Asn Asn Pro Leu Leu Glu Val Ser Asp Ser Ala Gly Leu Gln Gly Arg
595 600 605
Pro Ile Asp Arg Asn Ala Val Ser Phe Lys Ser Gly Asp Ala Thr Ala
610 615 620
Ser Cys Asn Asn Ser Val Val Thr Gly Ser Arg Ala Val Val Gly Asp
625 630 635 640
Tyr Arg Ser Asp Cys Asn Met Ser Pro Leu Thr Met Tyr Asn Phe Leu
645 650 655
Asn Thr Ser Phe His Pro Ala Asp Ala Ser Val Phe Ser Thr Ser Thr
660 665 670
Ser Ala Ser Ser Tyr Thr Arg Ala Ser His Ser Ala Val Ser Leu Ile
675 680 685
Gly Ser Gly Ala Met Asn Phe Val Tyr Trp Phe Ser Ala Met Ser Leu
690 695 700
Met Gly Ser Phe Ile Val Ile Gly Ile Gly Tyr Ala Gly Ala Met Leu
705 710 715 720
Phe Asn Thr Ile Arg Arg Thr Leu Ser Leu Ile Gly Ala Val Pro Phe
725 730 735
Ala Ala Met Gly Phe Ile Ala Gly Val Ala Lys Val Ile Val Tyr Thr
740 745 750
Ile Ala Met Leu Val Glu Val Ile Gly Thr Ile Ile Leu Tyr Gln Leu
755 760 765
Ile Thr Arg Phe Leu Met Ala Val Pro Ala Leu Phe Glu Gln Pro Leu
770 775 780
Ala Asp Ser Leu Ser Ser Asn Glu Ser Ala Glu Leu Val Ala Gly Ala
785 790 795 800
Gly Met Gly Leu Val Gly Met Ala Leu Ala Thr Ser Gly Asn Trp Ala
805 810 815
Ile Ala Gly Met Val Ile Thr Val Ala Ser Ser Met Gly Val Ile Ile
820 825 830
Phe Thr Ile Ile Ala Met Lys Val Arg Gly Ser Leu Val Ser Gly Val
835 840 845
Asp Glu Ala Val Thr Ser Val Ile Asn Arg Phe Leu Asp Thr Gln Val
850 855 860
Ser Ser Ala Gly Ala Thr Ser Gly Asp Gly Met Met Arg Arg Ala Ala
865 870 875 880
Ala Thr Gly Leu Gly Ile Gly Ala Thr His Met Val Leu Asn Arg Asp
885 890 895
Gly Asp Gly Gly Gly Ser Asp Ser Gly Ser Gly Gly Ser Gly Gly Gly
900 905 910
Ser Asp Ser Gly Leu Gly Glu Lys Ala Ala Gly Leu Ala Lys Val Val
915 920 925
Thr Thr Val Ala Gly Ala Gly Leu Val Gly Lys Tyr Ala Thr Asp Ala
930 935 940
Leu Asp Asn Tyr Ala Asp Gly Val Ile Asn Gly Asp Gly Asp Gly Ala
945 950 955 960
Phe Ala Ala Gly Gly Asp Ala Thr Val Asp Gly Asp Tyr Val Ala Asp
965 970 975
Gly Asp Ala Ile Ala Ser Ala Asp Ala Asn Ala Asp Phe Val Asp Gly
980 985 990
Val Val Asp Gly Ala Gly Arg Ala Ser Phe Ser Asn Ala Ala Tyr Ser
995 1000 1005
Ser Asp Gly Thr Thr Leu Asp Gly Glu Gly Ala Ser Val Asp Ala Gln
1010 1015 1020
Gly Asn Pro Leu His Ala Asp Gly Thr Pro Met Ser Ala Ala Glu Ala
1025 1030 1035 1040
Glu Met Lys Met Ala Gly Leu Ser Ser Ser Gly Thr Met Met Glu Lys
1045 1050 1055
Ser Gly Val Lys Ser Ser Gly Ile Thr Thr Ala Ala Asp Val Met Asp
1060 1065 1070
Asp Gln Ser Leu Ala Ser Ser Val Thr Glu Ser Gly Leu Ser Lys Ile
1075 1080 1085
Pro Asp Thr Tyr Gly Ala Asp Val Ser Gly Ala Ala Gly Thr Val Gly
1090 1095 1100
Thr Thr Gly Ala Asp Tyr Ser Ala Thr Asp Ser Ser Ala Gly Leu Asn
1105 1110 1115 1120
Met Ser Glu Ala Ala Leu Gln Ser Gly Thr Pro Met Gly Ala Leu Ala
1125 1130 1135
Gly Gly Ser Val Ser Ser Ser Asp Gln Ala Met Asn Asp Ala Ala Leu
1140 1145 1150
Gln Ile Ala Ala Ser Gln Gly Leu Ala Pro Ala Gly Ser Ile Ala Gly
1155 1160 1165
Met Glu Gln Leu Ser Ala Gln Ala Thr Glu Ala Pro Ala Gly Lys Ala
1170 1175 1180
Gly Lys Gln Leu Gly Asp Leu Ser Gly Ser Ala Leu Asn Thr Gln Leu
1185 1190 1195 1200
Ala Ser Met Gly Gln Gln Val Gly Asp Ser Val Asn Ser Ala Tyr Ala
1205 1210 1215
Ala Gly Gly Met Gly Gly Val Asp Val Ala Gly Lys Val Thr Glu Ala
1220 1225 1230
Ala Gln His Leu Ser Gln Val Pro Gly Gln Ile Gln Asn Ala Val Thr
1235 1240 1245
Asn Ala Asp Ala Gly Ser Ser Gly Ala Ser Phe Gly Gln Met Ala Gln
1250 1255 1260
Gly Ala Ala Gly Ile Ala Gly Val Ala Gly Val Ile Gly Ala Ala Gly
1265 1270 1275 1280
Ala Ala Ser Ser Ala Ala Gln Gly Ala Gly Thr Val Gln Gly Ala Met
1285 1290 1295
Gly Asn Ala Ala Ala Gly Ala Gly Met Ile Asn Asn Ala Val Ser Gly
1300 1305 1310
Gly Ala Thr Gly Ser Thr Gly Ala Ala His Val Val Asn Ala Ser His
1315 1320 1325
Gly Pro Val Ala Pro Gly Gln Ala His Tyr Gln Glu Ser Gly His Ala
1330 1335 1340
Gln Ala Phe Val Gln Asn Asn Gln Ala Asn Thr Ala His Thr Ala Asn
1345 1350 1355 1360
Thr Arg Ala Pro Ser Ser Ala Gln Ile Met Gly Ala Asn Val Ala Gly
1365 1370 1375
Ser Leu Ala Ser Gln Ala Val Arg Gly Ile Gly Gln Pro Gly Gln Met
1380 1385 1390
Gly Ala Asn Val Arg Asp Ala Met Gly Gly Ser Gly Arg Ser Gly Gly
1395 1400 1405
Arg Gly Gly Ala Thr Gln Gly Gly Arg Gly Ala Gln Arg Ser Gly Val
1410 1415 1420
Ser Ala Lys Asn Gly Ile Arg Ala Gln Arg Gly Gln Lys Pro Ser Val
1425 1430 1435 1440
Thr Gly Gln Ala Met Asn Ala Ala Met Arg Ser Ala Ala Val Ser Gly
1445 1450 1455
Arg Met Ala Asn Met Asp Gly Asn Ser Val Gly Gly Thr Glu Ala Asp
1460 1465 1470
Pro Gln Gln Gly Ser Gly Val Thr Glu Lys Gly Asp Lys Gly Val Lys
1475 1480 1485
<210>193
<211>3895
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3865)
<223>RXA01770
<400>193
tgctcatgac aggtaattca acgcgttacc ctgctgcagc ataaaagaac ctgcctcaac 60
acacctccac tttttctact tttaagaaag ccacattctc atg cca att atc att 115
Met Pro Ile Ile Ile
1 5
gat aac ctc aat tct gac gac gac tcc acc atc ggc act gcc acc gaa 163
Asp Asn Leu Asn Ser Asp Asp Asp Ser Thr Ile Gly Thr Ala Thr Glu
10 15 20
tac aac cct gac act gac gcc gat tta ctt gat gct att aac gct gat 211
Tyr Asn Pro Asp Thr Asp Ala Asp Leu Leu Asp Ala Ile Asn Ala Asp
25 30 35
gct gac ctt gat ggc gac gcc acc atc agc act aac gcg aca gaa gaa 259
Ala Asp Leu Asp Gly Asp Ala Thr Ile Ser Thr Asn Ala Thr Glu Glu
40 45 50
ggt gta gac gca gca gct gaa aaa cct aag aaa aag cgt aaa gcc cct 307
Gly Val Asp Ala Ala Ala Glu Lys Pro Lys Lys Lys Arg Lys Ala Pro
55 60 65
gct ctg aag cct aaa gga ctc acg gca aag ttc ttc cac cgt gat crt 355
Ala Leu Lys Pro Lys Gly Leu Thr Ala Lys Phe Phe His Arg Asp Leu
70 75 80 85
act ggc gta ggt ggt agg acc ggt cgc ctc aac aag aac gta cac ccg 403
Thr Gly Val Gly Gly Arg Thr Gly Arg Leu Asn Lys Asn Val His Pro
90 95 100
acc aac cca gat ctg tcc tac cag cca gtc tct gat gtc tac acc cca 451
Thr Asn Pro Asp Leu Ser Tyr Gln Pro Val Ser Asp Val Tyr Thr Pro
105 110 115
caa tca gca gat cac aag ggt att aag acc cgc tac atc ctt acc cat 499
Gln Ser Ala Asp His Lys Gly Ile Lys Thr Arg Tyr Ile Leu Thr His
120 125 130
cca acc ccc gct gtt gtt ctc agt gag tcc atc agc aac gcg ttt cat 547
Pro Thr Pro Ala Val Val Leu Ser Glu Ser Ile Ser Asn Ala Phe His
135 140 145
gtc tct acc ctg cgt cgc aac aat aat gtc aac aac tct gat tcg gaa 595
Val Ser Thr Leu Arg Arg Asn Asn Asn Val Asn Asn Ser Asp Ser Glu
150 155 160 165
ttg gct gcc tgg ccg tac ctc tac caa ctc gat att ccg cag ctg gac 643
Leu Ala Ala Trp Pro Tyr Leu Tyr Gln Leu Asp Ile Pro Gln Leu Asp
170 175 180
cag atg att aat gtc gct gac atc tgt gat tac cat ttc cac gga tat 691
Gln Met Ile Asn Val Ala Asp Ile Cys Asp Tyr His Phe His Gly Tyr
185 190 195
aac ctg tgg gtg gat ttt acc ccg cag act atc gct cta cga tcc ggt 739
Asn Leu Trp Val Asp Phe Thr Pro Gln Thr Ile Ala Leu Arg Ser Gly
200 205 210
aag acg gta ctc gat gac ggt acc acc gcc tct gat aac acc act cat 787
Lys Thr Val Leu Asp Asp Gly Thr Thr Ala Ser Asp Asn Thr Thr His
215 220 225
gtc tat tac cgc gtc acc gtt cac gtt att gcc ggt caa gat cat gga 835
Val Tyr Tyr Arg Val Thr Val His Val Ile Ala Gly Gln Asp His Gly
230 235 240 245
tct act ctg ctt gat gac cag ggc aac cag gtg ctt gat agg gat gat 883
Ser Thr Leu Leu Asp Asp Gln Gly Asn Gln Val Leu Asp Arg Asp Asp
250 255 260
aat cct att tct acc cca agt atc aag cgt att ggc gct gtc act gat 931
Asn Pro Ile Ser Thr Pro Ser Ile Lys Arg Ile Gly Ala Val Thr Asp
265 270 275
ctt ttc gat cac aat cct ttt ggc ttc gct agt gta aac tct ttc gca 979
Leu Phe Asp His Asn Pro Phe Gly Phe Ala Ser Val Asn Ser Phe Ala
280 285 290
ttt gtc gat ttc tca tgg gac cca gcc acc acc ttg gtc gat atg ctc 1027
Phe Val Asp Phe Ser Trp Asp Pro Ala Thr Thr Leu Val Asp Met Leu
295 300 305
aac aac ctt gat tca tat ctc tct aat cac atc aat att gcc agc tcc 1075
Asn Asn Leu Asp Ser Tyr Leu Ser Asn His Ile Asn Ile Ala Ser Ser
310 315 320 325
cca acc ccc att gcc ctc gat atg gtt gtg ctc aat gaa tgg tct gaa 1123
Pro Thr Pro Ile Ala Leu Asp Met Val Val Leu Asn Glu Trp Ser Glu
330 335 340
aag tcc tat cag ctg tgc gaa cgc gtt gtt gca cag gca aag ctc atc 1171
Lys Ser Tyr Gln Leu Cys Glu Arg Val Val Ala Gln Ala Lys Leu Ile
345 350 355
aac agc aac aag atc acc gca cat gtg agt gat gtc atc aag caa aat 1219
Asn Ser Asn Lys Ile Thr Ala His Val Ser Asp Val Ile Lys Gln Asn
360 365 370
gcc cac aat atc ttg tgg ttt acc gaa cag atg aac cct ggc acc acc 1267
Ala His Asn Ile Leu Trp Phe Thr Glu Gln Met Asn Pro Gly Thr Thr
375 380 385
aac ctc agc gag gta cct atc tcc aag aag tct atg ctg ccg atg tct 1315
Asn Leu Ser Glu Val Pro Ile Ser Lys Lys Ser Met Leu Pro Met Ser
390 395 400 405
cgc cag cta cgt att ttg gag cac tac gat gta cca ctg acc gcg tac 1363
Arg Gln Leu Arg Ile Leu Glu His Tyr Asp Val Pro Leu Thr Ala Tyr
410 415 420
tct gct ctt ttc tgg act gtc agc gcc att aag aat gaa tct atg gtg 1411
Ser Ala Leu Phe Trp Thr Val Ser Ala Ile Lys Asn Glu Ser Met Val
425 430 435
cag tac ctt gtg cga cag aat atg cag ctc acc ttg agc tca aat ctg 1459
Gln Tyr Leu Val Arg Gln Asn Met Gln Leu Thr Leu Ser Ser Asn Leu
440 445 450
gat gca ctc aac tcc att gtc tcc cag ctc cct gta cca gat aag gac 1507
Asp Ala Leu Asn Ser Ile Val Ser Gln Leu Pro Val Pro Asp Lys Asp
455 460 465
gtt gtg gct gct tct ggc tac cag att cag cca cac ttc tcc acg cag 1555
Val Val Ala Ala Ser Gly Tyr Gln Ile Gln Pro His Phe Ser Thr Gln
470 475 480 485
cag cgt gaa gca atc acc act gat aat cct ctc gcg att att cag gcc 1603
Gln Arg Glu Ala Ile Thr Thr Asp Asn Pro Leu Ala Ile Ile Gln Ala
490 495 500
ggt gct ggt acc ggt aaa tcc acc gtg att ctg gaa cgc att gag tac 1651
Gly Ala Gly Thr Gly Lys Ser Thr Val Ile Leu Glu Arg Ile Glu Tyr
505 510 515
ttg tgc gct gca ggc acc aac cct gaa gag att gct gtg ctg tct ttt 1699
Leu Cys Ala Ala Gly Thr Asn Pro Glu Glu Ile Ala Val Leu Ser Phe
520 525 530
act aat gct gcc gcc gat aac atc aca gca aaa aat gac aag gtg acc 1747
Thr Asn Ala Ala Ala Asp Asn Ile Thr Ala Lys Asn Asp Lys Val Thr
535 540 545
tct atg acc atc tcc aag atg gtt cat gag att tac gca cac aat ttc 1795
Ser Met Thr Ile Ser Lys Met Val His Glu Ile Tyr Ala His Asn Phe
550 555 560 565
cct gat cat gag atc tcc acc atc gac acc att atc aat acg ctt gat 1843
Pro Asp His Glu Ile Ser Thr Ile Asp Thr Ile Ile Asn Thr Leu Asp
570 575 580
att gaa tac ggc gat caa atg gtg acc tcc gat tac atg att cag ctc 1891
Ile Glu Tyr Gly Asp Gln Met Val Thr Ser Asp Tyr Met Ile Gln Leu
585 590 595
cgt gac ctg ctc tat aag gtc atg acg cag ggt ggc aac gcc aac ctg 1939
Arg Asp Leu Leu Tyr Lys Val Met Thr Gln Gly Gly Asn Ala Asn Leu
600 605 610
aca gcg ttg agc atc ttc atg gaa tcc cat atc gag gcg ttc atc tcg 1987
Thr Ala Leu Ser Ile Phe Met Glu Ser His Ile Glu Ala Phe Ile Ser
615 620 625
gtg ttg gat cag atc aag cag act tct ctt gag ctg gaa atc atc atc 2035
Val Leu Asp Gln Ile Lys Gln Thr Ser Leu Glu Leu Glu Ile Ile Ile
630 635 640 645
tgc tac ctg ctc ttg gat aag ctg atc gag cct cat gca tcg ccg aag 2083
Cys Tyr Leu Leu Leu Asp Lys Leu Ile Glu Pro His Ala Ser Pro Lys
650 655 660
tac ctc att att gat gag gtg cag gac aac tcg gtc ttc gag ttc gtc 2131
Tyr Leu Ile Ile Asp Glu Val Gln Asp Asn Ser Val Phe Glu Phe Val
665 670 675
ttt gca ctt cgt ttt gcg gca aag cac aat acg agt ctg tac ttg gtg 2179
Phe Ala Leu Arg Phe Ala Ala Lys His Asn Thr Ser Leu Tyr Leu Val
680 685 690
ggt gac tcg tca cag acc ttg tat gag ttc cgt tct gct aac cct aag 2227
Gly Asp Ser Ser Gln Thr Leu Tyr Glu Phe Arg Ser Ala Asn Pro Lys
695 700 705
gct ctg aac tcc ctg gaa gca tct ggt gtt ttc ggt acc tac cgt ttg 2275
Ala Leu Asn Ser Leu Glu Ala Ser Gly Val Phe Gly Thr Tyr Arg Leu
710 715 720 725
acc acc aat tac cgt tcc aac cag gaa att ctt gac ttc gct aat atc 2323
Thr Thr Asn Tyr Arg Ser Asn Gln Glu Ile Leu Asp Phe Ala Asn Ile
730 735 740
cac ctc tcg gat att gaa gct aac cag ttc gca ggt att cag ctc tac 2371
His Leu Ser Asp Ile Glu Ala Asn Gln Phe Ala Gly Ile Gln Leu Tyr
745 750 755
gcc aac tct ttt gat gca cct act gcg gac agc ttc aaa gaa aag gtt 2419
Ala Asn Ser Phe Asp Ala Pro Thr Ala Asp Ser Phe Lys Glu Lys Val
760 765 770
gaa ctg gac atg cac cat gtg tct aag cag tct gag ttt acc gac agc 2467
Glu Leu Asp Met His His Val Ser Lys Gln Ser Glu Phe Thr Asp Ser
775 780 785
att cct tat ttc atg gag tcc aat aaa gct cgc ttt gat gct gcg atc 2515
Ile Pro Tyr Phe Met Glu Ser Asn Lys Ala Arg Phe Asp Ala Ala Ile
790 795 800 805
ttg aac aat gag caa acc att gtg ctt gct cat tct ggt cgt gaa att 2563
Leu Asn Asn Glu Gln Thr Ile Val Leu Ala His Ser Gly Arg Glu Ile
810 815 820
cgt gca gcg cag caa gcg cta gcc gag atg tac ccc agc atc acg gtg 2611
Arg Ala Ala Gln Gln Ala Leu Ala Glu Met Tyr Pro Ser Ile Thr Val
825 830 835
cgt aac ttg cag tcc gat aag ggt ttc aac aac acg gta ttt tcg acc 2659
Arg Asn Leu Gln Ser Asp Lys Gly Phe Asn Asn Thr Val Phe Ser Thr
840 845 850
ttt att aag gat ttc tgg ttt gag gtc acc gct gta gac cca gct cat 2707
Phe Ile Lys Asp Phe Trp Phe Glu Val Thr Ala Val Asp Pro Ala His
855 860 865
gcc gca ttt acc ttt acc agc caa gtc acc gct cat cta gat aag ttg 2755
Ala Ala Phe Thr Phe Thr Ser Gln Val Thr Ala His Leu Asp Lys Leu
870 875 880 885
gta cgc gga aag cgt gag cag atg gag gat cgc gtt atc cgg tct atg 2803
Val Arg Gly Lys Arg Glu Gln Met Glu Asp Arg Val Ile Arg Ser Met
890 895 900
gct gca tgg tgg cgt gaa aat gag cgt gac atc cag ggt tgg gtg cag 2851
Ala Ala Trp Trp Arg Glu Asn Glu Arg Asp Ile Gln Gly Trp Val Gln
905 910 915
caa act cag tcc ggt gcg atc acc aac gaa gag ttc ttc tac cgt ctt 2899
Gln Thr Gln Ser Gly Ala Ile Thr Asn Glu Glu Phe Phe Tyr Arg Leu
920 925 930
cgt cag tgc att ctt gac tat gaa atc agg aat aac cga gca cgt cag 2947
Arg Gln Cys Ile Leu Asp Tyr Glu Ile Arg Asn Asn Arg Ala Arg Gln
935 940 945
tcc atg ctc aat gcg cgc aac aac gct aac aag gag gct gtc gca cag 2995
Ser Met Leu Asn Ala Arg Asn Asn Ala Asn Lys Glu Ala Val Ala Gln
950 955 960 965
gag aaa cca ctg ctc atg gtc tcc acc att cac agt gca aag ggt ctg 3043
Glu Lys Pro Leu Leu Met Val Ser Thr Ile His Ser Ala Lys Gly Leu
970 975 980
gaa ttc gat aat gtc atc gtg ctt caa aaa cca agc tcc gat gca gag 3091
Glu Phe Asp Asn Val Ile Val Leu Gln Lys Pro Ser Ser Asp Ala Glu
985 990 995
atg acc gaa gaa ggt aag cgc gca acc tat gtg gcg ctg acc cgt gca 3139
Met Thr Glu Glu Gly Lys Arg Ala Thr Tyr Val Ala Leu Thr Arg Ala
1000 1005 1010
aaa aag cgt gaa ctc att att gct ggt tct act cgc gca tac cca cgc 3187
Lys Lys Arg Glu Leu Ile Ile Ala Gly Ser Thr Arg Ala Tyr Pro Arg
1015 1020 1025
atc gtc acc gat tat gag caa atc gtt gat ctt ctt gaa aag cgc gat 3235
Ile Val Thr Asp Tyr Glu Gln Ile Val Asp Leu Leu Glu Lys Arg Asp
1030 1035 1040 1045
gag gag cgc aaa att aag gag gaa gag gct gca gca ctc gcc acc ctt 3283
Glu Glu Arg Lys Ile Lys Glu Glu Glu Ala Ala Ala Leu Ala Thr Leu
1050 1055 1060
gaa gct gaa cag gag acg cgc gcc ctt gct gct gct gag gcg cag gct 3331
Glu Ala Glu Gln Glu Thr Arg Ala Leu Ala Ala Ala Glu Ala Gln Ala
1065 1070 1075
cag gcg ttg ttg ctt gag cac aac ccg tgg ttg cgt gat ctc agc gat 3379
Gln Ala Leu Leu Leu Glu His Asn Pro Trp Leu Arg Asp Leu Ser Asp
1080 1085 1090
gaa gaa gtc act gct cta act gag caa gag atc atc aac aat gtt gag 3427
Glu Glu Val Thr Ala Leu Thr Glu Gln Glu Ile Ile Asn Asn Val Glu
1095 1100 1105
gca gcc cta cag att gaa gaa gag gag gaa gag gct cgt gca ctc gct 3475
Ala Ala Leu Gln Ile Glu Glu Glu Glu Glu Glu Ala Arg Ala Leu Ala
1110 1115 1120 1125
gca gca gag cca gca att cag cag tac ttg tct cag ttt gct ttc gat 3523
Ala Ala Glu Pro Ala Ile Gln Gln Tyr Leu Ser Gln Phe Ala Phe Asp
1130 1135 1140
gag ttc ccg gac gac gac aat gta gcc aac act gtc gta cat gta gca 3571
Glu Phe Pro Asp Asp Asp Asn Val Ala Asn Thr Val Val His Val Ala
1145 1150 1155
cca cag cct att ctc cac cag gca gtg cct gca gat gtg act gta caa 3619
Pro Gln Pro Ile Leu His Gln Ala Val Pro Ala Asp Val Thr Val Gln
1160 1165 1170
tca agc acc gcc ccc gta aca cct gtt gtt gcg gat ctt gag gta acc 3667
Ser Ser Thr Ala Pro Val Thr Pro Val Val Ala Asp Leu Glu Val Thr
1175 1180 1185
act gtt gca gct gat cca gtc gaa ccg act atc gtt gct gca cag cct 3715
Thr Val Ala Ala Asp Pro Val Glu Pro Thr Ile Val Ala Ala Gln Pro
1190 1195 1200 1205
gag gtg gac gac aac ctt gtc tac agc acg tcc acc cct aat agt cac 3763
Glu Val Asp Asp Asn Leu Val Tyr Ser Thr Ser Thr Pro Asn Ser His
1210 1215 1220
agt gac gtt att gca gtg aac tct gat acc tca gaa aac gct gct gtt 3811
Ser Asp Val Ile Ala Val Asn Ser Asp Thr Ser Glu Asn Ala Ala Val
1225 1230 1235
aat cca gtt ctg tcc gat att gag gca ctc cga gca att ttc aac aac 3859
Asn Pro Val Leu Ser Asp Ile Glu Ala Leu Arg Ala Ile Phe Asn Asn
1240 1245 1250
cag gac taaaacacct gctgctgttg agcgcttctc 3895
Gln Asp
1255
<210>194
<211>1255
<212>PRT
<213>谷氨酸棒状杆菌
<400>194
Met Pro Ile Ile Ile Asp Asn Leu Asn Ser Asp Asp Asp Ser Thr Ile
1 5 10 15
Gly Thr Ala Thr Glu Tyr Asn Pro Asp Thr Asp Ala Asp Leu Leu Asp
20 25 30
Ala Ile Asn Ala Asp Ala Asp Leu Asp Gly Asp Ala Thr Ile Ser Thr
35 40 45
Asn Ala Thr Glu Glu Gly Val Asp Ala Ala Ala Glu Lys Pro Lys Lys
50 55 60
Lys Arg Lys Ala Pro Ala Leu Lys Pro Lys Gly Leu Thr Ala Lys Phe
65 70 75 80
Phe His Arg Asp Leu Thr Gly Val Gly Gly Arg Thr Gly Arg Leu Asn
85 90 95
Lys Asn Val His Pro Thr Asn Pro Asp Leu Ser Tyr Gln Pro Val Ser
100 105 110
Asp Val Tyr Thr Pro Gln Ser Ala Asp His Lys Gly Ile Lys Thr Arg
115 120 125
Tyr Ile Leu Thr His Pro Thr Pro Ala Val Val Leu Ser Glu Ser Ile
130 135 140
Ser Asn Ala Phe His Val Ser Thr Leu Arg Arg Asn Asn Asn Val Asn
145 150 155 160
Asn Ser Asp Ser Glu Leu Ala Ala Trp Pro Tyr Leu Tyr Gln Leu Asp
165 170 175
Ile Pro Gln Leu Asp Gln Met Ile Asn Val Ala Asp Ile Cys Asp Tyr
180 185 190
His Phe His Gly Tyr Asn Leu Trp Val Asp Phe Thr Pro Gln Thr Ile
195 200 205
Ala Leu Arg Ser Gly Lys Thr Val Leu Asp Asp Gly Thr Thr Ala Ser
210 215 220
Asp Asn Thr Thr His Val Tyr Tyr Arg Val Thr Val His Val Ile Ala
225 230 235 240
GIy Gln Asp His Gly Ser Thr Leu Leu Asp Asp Gln Gly Asn Gln Val
245 250 255
Leu Asp Arg Asp Asp Asn Pro Ile Ser Thr Pro Ser Ile Lys Arg Ile
260 265 270
Gly Ala Val Thr Asp Leu Phe Asp His Asn Pro Phe Gly Phe Ala Ser
275 280 285
Val Asn Ser Phe Ala Phe Val Asp Phe Ser Trp Asp Pro Ala Thr Thr
290 295 300
Leu Val Asp Met Leu Asn Asn Leu Asp Ser Tyr Leu Ser Asn His Ile
305 310 315 320
Asn Ile Ala Ser Ser Pro Thr Pro Ile Ala Leu Asp Met Val Val Leu
325 330 335
Asn Glu Trp Ser Glu Lys Ser Tyr Gln Leu Cys Glu Arg Val Val Ala
340 345 350
Gln Ala Lys Leu Ile Asn Ser Asn Lys Ile Thr Ala His Val Ser Asp
355 360 365
Val Ile Lys Gln Asn Ala His Asn Ile Leu Trp Phe Thr Glu Gln Met
370 375 380
Asn Pro Gly Thr Thr Asn Leu Ser Glu Val Pro Ile Ser Lys Lys Ser
385 390 395 400
Met Leu Pro Met Ser Arg Gln Leu Arg Ile Leu Glu His Tyr Asp Val
405 410 415
Pro Leu Thr Ala Tyr Ser Ala Leu Phe Trp Thr Val Ser Ala Ile Lys
420 425 430
Asn Glu Ser Met Val Gln Tyr Leu Val Arg Gln Asn Met Gln Leu Thr
435 440 445
Leu Ser Ser Asn Leu Asp Ala Leu Asn Ser Ile Val Ser Gln Leu Pro
450 455 460
Val Pro Asp Lys Asp Val Val Ala Ala Ser Gly Tyr Gln Ile Gln Pro
465 470 475 480
His Phe Ser Thr Gln Gln Arg Glu Ala Ile Thr Thr Asp Asn Pro Leu
485 490 495
Ala Ile Ile Gln Ala Gly Ala Gly Thr Gly Lys Ser Thr Val Ile Leu
500 505 510
Glu Ar9 Ile Glu Tyr Leu Cys Ala Ala Gly Thr Asn Pro Glu Glu Ile
515 520 525
Ala Val Leu Ser Phe Thr Asn Ala Ala Ala Asp Asn Ile Thr Ala Lys
530 535 540
Asn Asp Lys Val Thr Ser Met Thr Ile Ser Lys Met Val His Glu Ile
545 550 555 560
Tyr Ala His Asn Phe Pro Asp His Glu Ile Ser Thr Ile Asp Thr Ile
565 570 575
Ile Asn Thr Leu Asp Ile Glu Tyr Gly Asp Gln Met Val Thr Ser Asp
580 585 590
Tyr Met Ile Gln Leu Arg Asp Leu Leu Tyr Lys Val Met Thr Gln Gly
595 600 605
Gly Asn Ala Asn Leu Thr Ala Leu Ser Ile Phe Met Glu Ser His Ile
610 615 620
Glu Ala Phe Ile Ser Val Leu Asp Gln Ile Lys Gln Thr Ser Leu Glu
625 630 635 640
Leu Glu Ile Ile Ile Cys Tyr Leu Leu Leu Asp Lys Leu Ile Glu Pro
645 650 655
His Ala Ser Pro Lys Tyr Leu Ile Ile Asp Glu Val Gln Asp Asn Ser
660 665 670
Val Phe Glu Phe Val Phe Ala Leu Arg Phe Ala Ala Lys His Asn Thr
675 680 685
Ser Leu Tyr Leu Val Gly Asp Ser Ser Gln Thr Leu Tyr Glu Phe Arg
690 695 700
Ser Ala Asn Pro Lys Ala Leu Asn Ser Leu Glu Ala Ser Gly Val Phe
705 710 715 720
Gly Thr Tyr Arg Leu Thr Thr Asn Tyr Arg Ser Asn Gln Glu Ile Leu
725 730 735
Asp Phe Ala Asn Ile His Leu Ser Asp Ile Glu Ala Asn Gln Phe Ala
740 745 750
Gly Ile Gln Leu Tyr Ala Asn Ser Phe Asp Ala Pro Thr Ala Asp Ser
755 760 765
Phe Lys Glu Lys Val Glu Leu Asp Met His His Val Ser Lys Gln Ser
770 775 780
Glu Phe Thr Asp Ser Ile Pro Tyr Phe Met Glu Ser Asn Lys Ala Arg
785 790 795 800
Phe Asp Ala Ala Ile Leu Asn Asn Glu Gln Thr Ile Val Leu Ala His
805 810 815
Ser Gly Arg Glu Ile Arg Ala Ala Gln Gln Ala Leu Ala Glu Met Tyr
820 825 830
Pro Ser Ile Thr Val Arg Asn Leu Gln Ser Asp Lys Gly Phe Asn Asn
835 840 845
Thr Val Phe Ser Thr Phe Ile Lys Asp Phe Trp Phe Glu Val Thr Ala
850 855 860
Val Asp Pro Ala His Ala Ala Phe Thr Phe Thr Ser Gln Val Thr Ala
865 870 875 880
His Leu Asp Lys Leu Val Arg Gly Lys Arg Glu Gln Met Glu Asp Arg
885 890 895
Val Ile Arg Ser Met Ala Ala Trp Trp Arg Glu Asn Glu Arg Asp Ile
900 905 910
Gln Gly Trp Val Gln Gln Thr Gln Ser Gly Ala Ile Thr Asn Glu Glu
915 920 925
Phe Phe Tyr Arg Leu Arg Gln Cys Ile Leu Asp Tyr Glu Ile Arg Asn
930 935 940
Asn Arg Ala Arg Gln Ser Met Leu Asn Ala Arg Asn Asn Ala Asn Lys
945 950 955 960
Glu Ala Val Ala Gln Glu Lys Pro Leu Leu Met Val Ser Thr Ile His
965 970 975
Ser Ala Lys Gly Leu Glu Phe Asp Asn Val Ile Val Leu Gln Lys Pro
980 985 990
Ser Ser Asp Ala Glu Met Thr Glu Glu Gly Lys Arg Ala Thr Tyr Val
995 1000 1005
Ala Leu Thr Arg Ala Lys Lys Arg Glu Leu Ile Ile Ala Gly Ser Thr
1010 1015 1020
Arg Ala Tyr Pro Arg Ile Val Thr Asp Tyr Glu Gln Ile Val Asp Leu
1025 1030 1035 1040
Leu Glu Lys Arg Asp Glu Glu Arg Lys Ile Lys Glu Glu Glu Ala Ala
1045 1050 1055
Ala Leu Ala Thr Leu Glu Ala Glu Gln Glu Thr Arg Ala Leu Ala Ala
1060 1065 1070
Ala Glu Ala Gln Ala Gln Ala Leu Leu Leu Glu His Asn Pro Trp Leu
1075 1080 1085
Arg Asp Leu Ser Asp Glu Glu Val Thr Ala Leu Thr Glu Gln Glu Ile
1090 1095 1100
Ile Asn Asn Val Glu Ala Ala Leu Gln Ile Glu Glu Glu Glu Glu Glu
1105 1110 1115 1120
Ala Arg Ala Leu Ala Ala Ala Glu Pro Ala Ile Gln Gln Tyr Leu Ser
1125 1130 1135
Gln Phe Ala Phe Asp Glu Phe Pro Asp Asp Asp Asn Val Ala Asn Thr
1140 1145 1150
Val Val His Val Ala Pro Gln Pro Ile Leu His Gln Ala Val Pro Ala
1155 1160 1165
Asp Val Thr Val Gln Ser Ser Thr Ala Pro Val Thr Pro Val Val Ala
1170 1175 1180
Asp Leu Glu Val Thr Thr Val Ala Ala Asp Pro Val Glu Pro Thr Ile
1185 1190 1195 1200
Val Ala Ala Gln Pro Glu Val Asp Asp Asn Leu Val Tyr Ser Thr Ser
1205 1210 1215
Thr Pro Asn Ser His Ser Asp Val Ile Ala Val Asn Ser Asp Thr Ser
1220 1225 1230
Glu Asn Ala Ala Val Asn Pro Val Leu Ser Asp Ile Glu Ala Leu Arg
1235 1240 1245
Ala Ile Phe Asn Asn Gln Asp
1250 1255
<210>195
<211>874
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(844)
<223>RXA01771
<400>195
ccggcccgat cctaaaacta taagttttgg gggacgcacc gctgccccgc tgttggtggt 60
tccggagcgg cgataatcgc taaacgtttg aggtgattgt atg ggt att ttc agg 115
Met Gly Ile Phe Arg
1 5
tcg cgg tcg cag gat ttt ggg tgg agc atg cac cag gct ggc cag ctc 163
Ser Arg Ser Gln Asp Phe Gly Trp Ser Met His Gln Ala Gly Gln Leu
10 15 20
atc aat gat ccc agt cag ggt ctg tgg cgc act tct gcc ctg cgc tcg 211
Ile Asn Asp Pro Ser Gln Gly Leu Trp Arg Thr Ser Ala Leu Arg Ser
25 30 35
ecg gtt gct cgg gtt gga cat gcc gtg ttg cgc cag cgt gcc ggt gag 259
Pro Val Ala Arg Val Gly His Ala Val Leu Arg Gln Arg Ala Gly Glu
40 45 50
atc tcg cgc atg caa ggt cgt gag ttt tct cgc cct ggg gat cag ttc 307
Ile Ser Arg Met Gln Gly Arg Glu Phe Ser Arg Pro Gly Asp Gln Phe
55 60 65
cga cag gta gat ttg cgc agg cga ctg att cag gtc cat ccc caa tca 355
Arg Gln Val Asp Leu Arg Arg Arg Leu Ile Gln Val His Pro Gln Ser
70 75 80 85
att cct aca gcg gat gcg atg gcc gta acc atc acc atg gcg ctc acc 403
Ile Pro Thr Ala Asp Ala Met Ala Val Thr Ile Thr Met Ala Leu Thr
90 95 100
gct gcc acg att gat ccg gtg aag ttc gtc gcg gat tca cag aac ccg 451
Ala Ala Thr Ile Asp Pro Val Lys Phe Val Ala Asp Ser Gln Asn Pro
105 110 115
gat gaa gag att tat ttg gca gct cag atc gca ttg cgg gaa atg gtt 499
Asp Glu Glu Ile Tyr Leu Ala Ala Gln Ile Ala Leu Arg Glu Met Val
120 125 130
atc gct atg cct ttg gag gat ttc atc ggg gtg cgc att gat cta gag 547
Ile Ala Met Pro Leu Glu Asp Phe Ile Gly Val Arg Ile Asp Leu Glu
135 140 145
cct gtt ttg gtg gct gct caa gct gct gcg aag aat gtg ggc gtg gaa 595
Pro Val Leu Val Ala Ala Gln Ala Ala Ala Lys Asn Val Gly Val Glu
150 155 160 165
gtc tcg tca atc ttg ctg aag gat ctg aat ctt ccc cag gag tac tcg 643
Val Ser Ser Ile Leu Leu Lys Asp Leu Asn Leu Pro Gln Glu Tyr Ser
170 175 180
gga gcg ttg cag gaa tcg atc gtt gcg aaa att caa gcc gaa act gat 691
Gly Ala Leu Gln Glu Ser Ile Val Ala Lys Ile Gln Ala Glu Thr Asp
185 190 195
ctg gaa cgt gca cga aat gaa gtg aaa act acc cgt gct cga ctt gcc 739
Leu Glu Arg Ala Arg Asn Glu Val Lys Thr Thr Arg Ala Arg Leu Ala
200 205 210
agc gcg aaa gtg ttg gag caa aat ccg att ctt gcc aaa att cgg atg 787
Ser Ala Lys Val Leu Glu Gln Asn Pro Ile Leu Ala Lys Ile Arg Met
215 220 225
att gaa gcg ctc cca ccg gga tcc aca att gag gtt cgg gag ggt gac 835
Ile Glu Ala Leu Pro Pro Gly Ser Thr Ile Glu Val Arg Glu Gly Asp
230 235 240 245
tca aag gca taaagttgcc catttcggtg ccctccacgg 874
Ser Lys Ala
<210>196
<211>248
<212>PRT
<213>谷氨酸棒状杆菌
<400>196
Met Gly Ile Phe Arg Ser Arg Ser Gln Asp Phe Gly Trp Ser Met His
1 5 10 15
Gln Ala Gly Gln Leu Ile Asn Asp Pro Ser Gln Gly Leu Trp Arg Thr
20 25 30
Ser Ala Leu Arg Ser Pro Val Ala Arg Val Gly His Ala Val Leu Arg
35 40 45
Gln Arg Ala Gly Glu Ile Ser Arg Met Gln Gly Arg Glu Phe Ser Arg
50 55 60
Pro Gly Asp Gln Phe Arg Gln Val Asp Leu Arg Arg Arg Leu Ile Gln
65 70 75 80
Val His Pro Gln Ser Ile Pro Thr Ala Asp Ala Met Ala Val Thr Ile
85 90 95
Thr Met Ala Leu Thr Ala Ala Thr Ile Asp Pro Val Lys Phe Val Ala
100 105 110
Asp Ser Gln Asn Pro Asp Glu Glu Ile Tyr Leu Ala Ala Gln Ile Ala
115 120 125
Leu Arg Glu Met Val Ile Ala Met Pro Leu Glu Asp Phe Ile Gly Val
130 135 140
Arg Ile Asp Leu Glu Pro Val Leu Val Ala Ala Gln Ala Ala Ala Lys
145 150 155 160
Asn Val Gly Val Glu Val Ser Ser Ile Leu Leu Lys Asp Leu Asn Leu
165 170 175
Pro Gln Glu Tyr Ser Gly Ala Leu Gln Glu Ser Ile Val Ala Lys Ile
180 185 190
Gln Ala Glu Thr Asp Leu Glu Arg Ala Arg Asn Glu Val Lys Thr Thr
195 200 205
Arg Ala Arg Leu Ala Ser Ala Lys Val Leu Glu Gln Asn Pro Ile Leu
210 215 220
Ala Lys Ile Arg Met Ile Glu Ala Leu Pro Pro Gly Ser Thr Ile Glu
225 230 235 240
Val Arg Glu Gly Asp Ser Lys Ala
245
<210>197
<211>1582
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1552)
<223>RXA01776
<400>197
cgtataacca cacacgccac agatactcag ctatctgtgg cgtgttgttt tacatcaacc 60
tctcacccat cccccatcac tattaaagaa aatcaccatc atg acc cac act gat 115
Met Thr His Thr Asp
1 5
acc cca gcc cct gca cac ggc gta aca ccg agt gaa ttc gct gtt acc 163
Thr Pro Ala Pro Ala His Gly Val Thr Pro Ser Glu Phe Ala Val Thr
10 15 20
tca cac gaa ctc acc acc tca cca aca cct agt ccg aca cag gtc gct 211
Ser His Glu Leu Thr Thr Ser Pro Thr Pro Ser Pro Thr Gln Val Ala
25 30 35
att gca gcc cag gaa aaa att cgc cag cgc aag ctc gaa gag ctt aaa 259
Ile Ala Ala Gln Glu Lys Ile Arg Gln Arg Lys Leu Glu Glu Leu Lys
40 45 50
gca aaa aag cgc aca gac acg aca gct caa cca ctt cta cct gca cct 307
Ala Lys Lys Arg Thr Asp Thr Thr Ala Gln Pro Leu Leu Pro Ala Pro
55 60 65
gtg ctg cat aca cgc cca ctc ccc tca ccg aat cca ccg cga cca tct 355
Val Leu His Thr Arg Pro Leu Pro Ser Pro Asn Pro Pro Arg Pro Ser
70 75 80 85
cca ccg gca cct acc act gga cac tgg gaa ctt ctt att gtc agg tca 403
Pro Pro Ala Pro Thr Thr Gly His Trp Glu Leu Leu Ile Val Arg Ser
90 95 100
cag cag aag aaa atg acc gca gcc aat ccc ttt ctc aaa ttc ctt cat 451
Gln Gln Lys Lys Met Thr Ala Ala Asn Pro Phe Leu Lys Phe Leu His
105 110 115
ctc ggt aaa gcc ttc aac gtg aaa gac cta ggg ctc acc tgc gat cgc 499
Leu Gly Lys Ala Phe Asn Val Lys Asp Leu Gly Leu Thr Cys Asp Arg
120 125 130
acc act gat gtt gac ctc tca cgg cta ggc aag ctc aat ccg ccc agc 547
Thr Thr Asp Val Asp Leu Ser Arg Leu Gly Lys Leu Asn Pro Pro Ser
135 140 145
tca ctt atc ctt gaa cta gca acg gca ctt gac ctg cat cca ttc ctc 595
Ser Leu Ile Leu Glu Leu Ala Thr Ala Leu Asp Leu His Pro Phe Leu
150 155 160 165
tca gtc gca ctc tct tcg cac agt gcg gtc aac gcc cgt cgc aac caa 643
Ser Val Ala Leu Ser Ser His Ser Ala Val Asn Ala Arg Arg Asn Gln
170 175 180
ctt gaa tta acc ctg cac aac cat gac act cag ggc aca gca tta ggt 691
Leu Glu Leu Thr Leu His Asn His Asp Thr Gln Gly Thr Ala Leu Gly
185 190 195
cac agc gtt act atg gtc cag cta ccc gag gtc att aat cga gct gct 739
His Ser Val Thr Met Val Gln Leu Pro Glu Val Ile Asn Arg Ala Ala
200 205 210
gta gag ccc tat atc aaa ccc aag ggc tcg ggc aat gac ccg ctt gac 787
Val Glu Pro Tyr Ile Lys Pro Lys Gly Ser Gly Asn Asp Pro Leu Asp
215 220 225
caa agc ctg aac cac cgc gca ccc acc gta agt gac gtg caa aaa gcc 835
Gln Ser Leu Asn His Arg Ala Pro Thr Val Ser Asp Val Gln Lys Ala
230 235 240 245
ctt gtg cag gaa aac aca ggc acg gca aca gac agc aac aac tac ctg 883
Leu Val Gln Glu Asn Thr Gly Thr Ala Thr Asp Ser Asn Asn Tyr Leu
250 255 260
ctt cct ttt gat cct gat tct ggt gca cca aaa acc acc gcg ctt aat 931
Leu Pro Phe Asp Pro Asp Ser Gly Ala Pro Lys Thr Thr Ala Leu Asn
265 270 275
gcg cct aat aag gat ctc cta cag gcc agg cat gag ctt gcc tat att 979
Ala Pro Asn Lys Asp Leu Leu Gln Ala Arg His Glu Leu Ala Tyr Ile
280 285 290
ccc gag atc ccc gcc att gca tcg tgg ctc gat cac ctt gac ccc atc 1027
Pro Glu Ile Pro Ala Ile Ala Ser Trp Leu Asp His Leu Asp Pro Ile
295 300 305
atg cgt gtg cac cgt agt ctt gcg ctt tca att ttc cca gtg cag ctt 1075
Met Arg Val His Arg Ser Leu Ala Leu Ser Ile Phe Pro Val Gln Leu
310 315 320 325
cta cgt gct gcc gtt gag aat ctc cct cat aac gta acc gat ctg cat 1123
Leu Arg Ala Ala Val Glu Asn Leu Pro His Asn Val Thr Asp Leu His
330 335 340
aat cac ctt gat cct gag gat ctg ctc cct gag gaa ctc acc acc tgg 1171
Asn His Leu Asp Pro Glu Asp Leu Leu Pro Glu Glu Leu Thr Thr Trp
345 350 355
ctc gat cac ctc ttc gat gca gac tta acc gct ctc gct tca tgc cca 1219
Leu Asp His Leu Phe Asp Ala Asp Leu Thr Ala Leu Ala Ser Cys Pro
360 365 370
cag ctt gca cca cct atc gtg cgc agc cac aat cac gcc aaa gcg atc 1267
Gln Leu Ala Pro Pro Ile Val Arg Ser His Asn His Ala Lys Ala Ile
375 380 385
gca gct atc acc gtg acc tct gtc atg gac gct ctc atc gcc aaa act 1315
Ala Ala Ile Thr Val Thr Ser Val Met Asp Ala Leu Ile Ala Lys Thr
390 395 400 405
ctt gat gcg cat gac gat cca ctg ccc tca cta ccg gag ttt gta gaa 1363
Leu Asp Ala His Asp Asp Pro Leu Pro Ser Leu Pro Glu Phe Val Glu
410 415 420
cta ctg cgt gac gct gta gcc cag ctc gca gcg cat ttt aag cag cag 1411
Leu Leu Arg Asp Ala Val Ala Gln Leu Ala Ala His Phe Lys Gln Gln
425 430 435
cgc ggc gag gta cta ccg cag ccc aat ggc ttg cga tac gcc acc gac 1459
Arg Gly Glu Val Leu Pro Gln Pro Asn Gly Leu Arg Tyr Ala Thr Asp
440 445 450
ttt ggc ctg ctc tct tat att gcc gat ggc tta cat gaa tat gca ctt 1507
Phe Gly Leu Leu Ser Tyr Ile Ala Asp Gly Leu His Glu Tyr Ala Leu
455 460 465
gcc atc gac ttc ggg ctg atc tac ccg aat gaa ctc atc acc gag 1552
Ala Ile Asp Phe Gly Leu Ile Tyr Pro Asn Glu Leu Ile Thr Glu
470 475 480
taaacccact cctagaaagg tctttaccca 1582
<210>198
<211>484
<212>PRT
<213>谷氨酸棒状杆菌
<400>198
Met Thr His Thr Asp Thr Pro Ala Pro Ala His Gly Val Thr Pro Ser
1 5 10 15
Glu Phe Ala Val Thr Ser His Glu Leu Thr Thr Ser Pro Thr Pro Ser
20 25 30
Pro Thr Gln Val Ala Ile Ala Ala Gln Glu Lys Ile Arg Gln Arg Lys
35 40 45
Leu Glu Glu Leu Lys Ala Lys Lys Arg Thr Asp Thr Thr Ala Gln Pro
50 55 60
Leu Leu Pro Ala Pro Val Leu His Thr Arg Pro Leu Pro Ser Pro Asn
65 70 75 80
Pro Pro Arg Pro Ser Pro Pro Ala Pro Thr Thr Gly His Trp Glu Leu
85 90 95
Leu Ile Val Arg Ser Gln Gln Lys Lys Met Thr Ala Ala Asn Pro Phe
100 105 110
Leu Lys Phe Leu His Leu Gly Lys Ala Phe Asn Val Lys Asp Leu Gly
115 120 125
Leu Thr Cys Asp Arg Thr Thr Asp Val Asp Leu Ser Arg Leu Gly Lys
130 135 140
Leu Asn Pro Pro Ser Ser Leu Ile Leu Glu Leu Ala Thr Ala Leu Asp
145 150 155 160
Leu His Pro Phe Leu Ser Val Ala Leu Ser Ser His Ser Ala Val Asn
165 170 175
Ala Arg Arg Asn Gln Leu Glu Leu Thr Leu His Asn His Asp Thr Gln
180 185 190
Gly Thr Ala Leu Gly His Ser Val Thr Met Val Gln Leu Pro Glu Val
195 200 205
Ile Asn Arg Ala Ala Val Glu Pro Tyr Ile Lys Pro Lys Gly Ser Gly
210 215 220
Asn Asp Pro Leu Asp Gln Ser Leu Asn His Arg Ala Pro Thr Val Ser
225 230 235 240
Asp Val Gln Lys Ala Leu Val Gln Glu Asn Thr Gly Thr Ala Thr Asp
245 250 255
Ser Asn Asn Tyr Leu Leu Pro Phe Asp Pro Asp Ser Gly Ala Pro Lys
260 265 270
Thr Thr Ala Leu Asn Ala Pro Asn Lys Asp Leu Leu Gln Ala Arg His
275 280 285
Glu Leu Ala Tyr Ile Pro Glu Ile Pro Ala Ile Ala Ser Trp Leu Asp
290 295 300
His Leu Asp Pro Ile Met Arg Val His Arg Ser Leu Ala Leu Ser Ile
305 310 315 320
Phe Pro Val Gln Leu Leu Arg Ala Ala Val Glu Asn Leu Pro His Asn
325 330 335
Val Thr Asp Leu His Asn His Leu Asp Pro Glu Asp Leu Leu Pro Glu
340 345 350
Glu Leu Thr Thr Trp Leu Asp His Leu Phe Asp Ala Asp Leu Thr Ala
355 360 365
Leu Ala Ser Cys Pro Gln Leu Ala Pro Pro Ile Val Arg Ser His Asn
370 375 380
His Ala Lys Ala Ile Ala Ala Ile Thr Val Thr Ser Val Met Asp Ala
385 390 395 400
Leu Ile Ala Lys Thr Leu Asp Ala His Asp Asp Pro Leu Pro Ser Leu
405 410 415
Pro Glu Phe Val Glu Leu Leu Arg Asp Ala Val Ala Gln Leu Ala Ala
420 425 430
His Phe Lys Gln Gln Arg Gly Glu Val Leu Pro Gln Pro Asn Gly Leu
435 440 445
Arg Tyr Ala Thr Asp Phe Gly Leu Leu Ser Tyr Ile Ala Asp Gly Leu
450 455 460
His Glu Tyr Ala Leu Ala Ile Asp Phe Gly Leu Ile Tyr Pro Asn Glu
465 470 475 480
Leu Ile Thr Glu
<210>199
<211>1267
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1237)
<223>RXA01778
<400>199
ctactacccc ctccgggtgt ggggtagctg taacaactac cccacaatct cagccggtct 60
tccatacata actgcgaaat tggttagagt ctttttcatg atg ctc tac caa cac 115
Met Leu Tyr Gln His
1 5
acc aca cgc ggg ctg atc att agt gac gcg atc atc gcg aaa atc agc 163
Thr Thr Arg Gly Leu Ile Ile Ser Asp Ala Ile Ile Ala Lys Ile Ser
10 15 20
caa gtc aac caa ctg ctg cta cta aga ccc acg ctg ttg caa gac aaa 211
Gln Val Asn Gln Leu Leu Leu Leu Arg Pro Thr Leu Leu Gln Asp Lys
25 30 35
gat atg cac cat gcg aca ttg agt agc gac acc cca tca att cgc agt 259
Asp Met His His Ala Thr Leu Ser Ser Asp Thr Pro Ser Ile Arg Ser
40 45 50
cgc cgt cgc tcc tat cgc gag aat aaa gcc gcc tat gcg acc acg agc 307
Arg Arg Arg Ser Tyr Arg Glu Asn Lys Ala Ala Tyr Ala Thr Thr Ser
55 60 65
acc atc aga aac cgt gag acc ttt ttc agc aca tct gat att gat ttc 355
Thr Ile Arg Asn Arg Glu Thr Phe Phe Ser Thr Ser Asp Ile Asp Phe
70 75 80 85
gcc ata ctc atg att cag gac gta caa gaa tct ctc gta tct ttt gac 403
Ala Ile Leu Met Ile Gln Asp Val Gln Glu Ser Leu Val Ser Phe Asp
90 95 100
ccc acc ttg gct caa gat atg aag cca aga acc aac gca gtc att tct 451
Pro Thr Leu Ala Gln Asp Met Lys Pro Arg Thr Asn Ala Val Ile Ser
105 110 115
gca ctc aat gag cta cgc gag cca ctg gat acc ttc tac cgt gcc cat 499
Ala Leu Asn Glu Leu Arg Glu Pro Leu Asp Thr Phe Tyr Arg Ala His
120 125 130
gaa tac ggt gac atc atc agc gat ggt gct tat gag ttc gat cat gct 547
Glu Tyr Gly Asp Ile Ile Ser Asp Gly Ala Tyr Glu Phe Asp His Ala
135 140 145
gcc att gat gca caa cac agc ctt agt gaa ctc gtt gcc ggt ctg cat 595
Ala Ile Asp Ala Gln His Ser Leu Ser Glu Leu Val Ala Gly Leu His
150 155 160 165
aat tat gac aac ctc atg ttt ccc cag gaa acc gct ccg ttg atg agt 643
Asn Tyr Asp Asn Leu Met Phe Pro Gln Glu Thr Ala Pro Leu Met Ser
170 175 180
gaa tcc aat ggc att cag ctg gct cag tac tat aag tcc acc aaa ctc 691
Glu Ser Asn Gly Ile Gln Leu Ala Gln Tyr Tyr Lys Ser Thr Lys Leu
185 190 195
gat gag tca caa ttg acg gtg gaa cct aat gag cct ggt atc agc att 739
Asp Glu Ser Gln Leu Thr Val Glu Pro Asn Glu Pro Gly Ile Ser Ile
200 205 210
atg tgg cac gcc cat gat ttc acc tgg tct tgc cta gtg cgc tat gac 787
Met Trp His Ala His Asp Phe Thr Trp Ser Cys Leu Val Arg Tyr Asp
215 220 225
gac atg aac cgt tac cct gag cat ttc agc att tct ttt aaa gac atg 835
Asp Met Asn Arg Tyr Pro Glu His Phe Ser Ile Ser Phe Lys Asp Met
230 235 240 245
ctg atc cct gaa cag atc tat act ttc cac ttc acc cca cgt gag gca 883
Leu Ile Pro Glu Gln Ile Tyr Thr Phe His Phe Thr Pro Arg Glu Ala
250 255 260
atg caa ctg cgc aag gta ctt ttt aat gcc atc gct gcc tac aag tct 931
Met Gln Leu Arg Lys Val Leu Phe Asn Ala Ile Ala Ala Tyr Lys Ser
265 270 275
cag aca tgg ggt gat gag aac act gat ctc acc aag gct gaa ctc gaa 979
Gln Thr Trp Gly Asp Glu Asn Thr Asp Leu Thr Lys Ala Glu Leu Glu
280 285 290
ttt ggt ggc acc tcc atg acc ttc cgc gcc ggc ctc tca cag ctt agc 1027
Phe Gly Gly Thr Ser Met Thr Phe Arg Ala Gly Leu Ser Gln Leu Ser
295 300 305
gtc acc gga cct cct gat cac cgc cag gtg ttc tgc ttg tcg tct tct 1075
Val Thr Gly Pro Pro Asp His Arg Gln Val Phe Cys Leu Ser Ser Ser
310 315 320 325
ggt cgt gag gat cac aac acc ccg cca aac atg cga gac gga cat gtc 1123
Gly Arg Glu Asp His Asn Thr Pro Pro Asn Met Arg Asp Gly His Val
330 335 340
att gat att gcg gtt act caa ttg tcc acc ctg cgt aac ctc att cgg 1171
Ile Asp Ile Ala Val Thr Gln Leu Ser Thr Leu Arg Asn Leu Ile Arg
345 350 355
gta ctg tgc acc act cac ccg gat cat tca cca gca ctg cta cct tat 1219
Val Leu Cys Thr Thr His Pro Asp His Ser Pro Ala Leu Leu Pro Tyr
360 365 370
ggt cag cag tac acc atc taacatgctc taactcgacc ccgaaacacc 1267
Gly Gln Gln Tyr Thr Ile
375
<210>200
<211>379
<212>PRT
<213>谷氨酸棒状杆菌
<400>200
Met Leu Tyr Gln His Thr Thr Arg Gly Leu Ile Ile Ser Asp Ala Ile
1 5 10 15
Ile Ala Lys Ile Ser Gln Val Asn Gln Leu Leu Leu Leu Arg Pro Thr
20 25 30
Leu Leu Gln Asp Lys Asp Met His His Ala Thr Leu Ser Ser Asp Thr
35 40 45
Pro Ser Ile Arg Ser Arg Arg Arg Ser Tyr Arg Glu Asn Lys Ala Ala
50 55 60
Tyr Ala Thr Thr Ser Thr Ile Arg Asn Arg Glu Thr Phe Phe Ser Thr
65 70 75 80
Ser Asp Ile Asp Phe Ala Ile Leu Met Ile Gln Asp Val Gln Glu Ser
85 90 95
Leu Val Ser Phe Asp Pro Thr Leu Ala Gln Asp Met Lys Pro Arg Thr
100 105 110
Asn Ala Val Ile Ser Ala Leu Asn Glu Leu Arg Glu Pro Leu Asp Thr
115 120 125
Phe Tyr Arg Ala His Glu Tyr Gly Asp Ile Ile Ser Asp Gly Ala Tyr
130 135 140
Glu Phe Asp His Ala Ala Ile Asp Ala Gln His Ser Leu Ser Glu Leu
145 150 155 160
Val Ala Gly Leu His Asn Tyr Asp Asn Leu Met Phe Pro Gln Glu Thr
165 170 175
Ala Pro Leu Met Ser Glu Ser Asn Gly Ile Gln Leu Ala Gln Tyr Tyr
180 185 190
Lys Ser Thr Lys Leu Asp Glu Ser Gln Leu Thr Val Glu Pro Asn Glu
195 200 205
Pro Gly Ile Ser Ile Met Trp His Ala His Asp Phe Thr Trp Ser Cys
210 215 220
Leu Val Arg Tyr Asp Asp Met Asn Arg Tyr Pro Glu His Phe Ser Ile
225 230 235 240
Ser Phe Lys Asp Met Leu Ile Pro Glu Gln Ile Tyr Thr Phe His Phe
245 250 255
Thr Pro Arg Glu Ala Met Gln Leu Arg Lys Val Leu Phe Asn Ala Ile
260 265 270
Ala Ala Tyr Lys Ser Gln Thr Trp Gly Asp Glu Asn Thr Asp Leu Thr
275 280 285
Lys Ala Glu Leu Glu Phe Gly Gly Thr Ser Met Thr Phe Arg Ala Gly
290 295 300
Leu Ser Gln Leu Ser Val Thr Gly Pro Pro Asp His Arg Gln Val Phe
305 310 315 320
Cys Leu Ser Ser Ser Gly Arg Glu Asp His Asn Thr Pro Pro Asn Met
325 330 335
Arg Asp Gly His Val Ile Asp Ile Ala Val Thr Gln Leu Ser Thr Leu
340 345 350
Arg Asn Leu Ile Arg Val Leu Cys Thr Thr His Pro Asp His Ser Pro
355 360 365
Ala Leu Leu Pro Tyr Gly Gln Gln Tyr Thr Ile
370 375
<210>201
<211>1549
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1519)
<223>RXA01779
<400>201
ttagtattta gtcaattagc caactacgat aaaactagtg aataaacata ctagttcact 60
ttgtcgcgct taaaatacta gaaagtaata taaaaaactc atg cgt tat act ccc 115
Met Arg Tyr Thr Pro
1 5
cgt acc cac cgc gca ctc gga ctt gct gca ctt acc gct aca gct ctc 163
Arg Thr His Arg Ala Leu Gly Leu Ala Ala Leu Thr Ala Thr Ala Leu
10 15 20
cta ctg agc tca tgc tcg acc acc gct gac ctt ctc ggc gcc gat tct 211
Leu Leu Ser Ser Cys Ser Thr Thr Ala Asp Leu Leu Gly Ala Asp Ser
25 30 35
gtg gcg cag gct gtc acc gat ggt ggc tct gcc ctt gat ccc act gca 259
Val Ala Gln Ala Val Thr Asp Gly Gly Ser Ala Leu Asp Pro Thr Ala
40 45 50
gct cat gtg ctc acc atc acc aat gcc act gca atg act ctt gct gag 307
Ala His Val Leu Thr Ile Thr Asn Ala Thr Ala Met Thr Leu Ala Glu
55 60 65
cta cca gaa aac acc gac cct gac gca gcc aat aac acg gag cca aca 355
Leu Pro Glu Asn Thr Asp Pro Asp Ala Ala Asn Asn Thr Glu Pro Thr
70 75 80 85
atg gcc caa agc gcc ctc aag cgt att tac ggt ctc gaa att gcc gcc 403
Met Ala Gln Ser Ala Leu Lys Arg Ile Tyr Gly Leu Glu Ile Ala Ala
90 95 100
gat ggc act ctg att gct gtc gca gcg cca tta acc tcg cga aat agt 451
Asp Gly Thr Leu Ile Ala Val Ala Ala Pro Leu Thr Ser Arg Asn Ser
105 110 115
ggt tct gag cag cct act gtc gca gca atg gct gat cca gaa aac aat 499
Gly Ser Glu Gln Pro Thr Val Ala Ala Met Ala Asp Pro Glu Asn Asn
120 125 130
gct gaa ctc acc aca gca cat att ctt gag atc gac cca gcc aca gga 547
Ala Glu Leu Thr Thr Ala His Ile Leu Glu Ile Asp Pro Ala Thr Gly
135 140 145
act gcc aca cca cgt caa gat att gct ctg act gaa gat ttt cag acc 595
Thr Ala Thr Pro Arg Gln Asp Ile Ala Leu Thr Glu Asp Phe Gln Thr
150 155 160 165
atg gct agc tct ttt agc cag atc cgt aat ggc tgg ggt act acc caa 643
Met Ala Ser Ser Phe Ser Gln Ile Arg Asn Gly Trp Gly Thr Thr Gln
170 175 180
ctc atc gga caa cac cct gat gat ccc aag caa cct att cgt tca gct 691
Leu Ile Gly Gln His Pro Asp Asp Pro Lys Gln Pro Ile Arg Ser Ala
185 190 195
gat acc tgg act gtc acc ggc tcc act cag atc acc ggc ttt aac acc 739
Asp Thr Trp Thr Val Thr Gly Ser Thr Gln Ile Thr Gly Phe Asn Thr
200 205 210
aac acc cca acg gga acc gaa gat gct gcc tac acc atg ccg gcg act 787
Asn Thr Pro Thr Gly Thr Glu Asp Ala Ala Tyr Thr Met Pro Ala Thr
215 220 225
gag cca gct gtg gga ctg tgc gct cta gaa tca ggc tcc gat gca cca 835
Glu Pro Ala Val Gly Leu Cys Ala Leu Glu Ser Gly Ser Asp Ala Pro
230 235 240 245
ctg gat gaa cac cgt gag ctc agc acc agt gca ttg cgt acc gca tca 883
Leu Asp Glu His Arg Glu Leu Ser Thr Ser Ala Leu Arg Thr Ala Ser
250 255 260
gta ttg tcg agc tct gga tct gcc acc ctc aag ctg cat gat cct atg 931
Val Leu Ser Ser Ser Gly Ser Ala Thr Leu Lys Leu His Asp Pro Met
265 270 275
gtc atg tct gct act ggc att gtc caa gca cgc gcc tat gtc gat ggc 979
Val Met Ser Ala Thr Gly Ile Val Gln Ala Arg Ala Tyr Val Asp Gly
280 285 290
gaa gtc att aac cag cac gag att ggc gat ctc cgc gag cag ctc ggt 1027
Glu Val Ile Asn Gln His Glu Ile Gly Asp Leu Arg Glu Gln Leu Gly
295 300 305
atc aac atc gaa gaa tct gag gct gct caa gca gaa tcg gat gcc caa 1075
Ile Asn Ile Glu Glu Ser Glu Ala Ala Gln Ala Glu Ser Asp Ala Gln
310 315 320 325
gca gcc acc gat cac cca ctt gca gcc ctg ggc tta agc aac ccc acc 1123
Ala Ala Thr Asp His Pro Leu Ala Ala Leu Gly Leu Ser Asn Pro Thr
330 335 340
agc tca gcg ttg gtt cca ggt ctt gct gag ctt gat tgt ctc agt gct 1171
Ser Ser Ala Leu Val Pro Gly Leu Ala Glu Leu Asp Cys Leu Ser Ala
345 350 355
gac cag gct gcc acc tgg cat aac cgc gac acc agc att ggc acc ggt 1219
Asp Gln Ala Ala Thr Trp His Asn Arg Asp Thr Ser Ile Gly Thr Gly
360 365 370
aag ccc agc gtg ctt gct gtc atc aac gcg gaa atg gcc gat gac ttt 1267
Lys Pro Ser Val Leu Ala Val Ile Asn Ala Glu Met Ala Asp Asp Phe
375 380 385
acc ctg cag ctg ttg agc tca ggt gcc acc act gcc gaa acg cag ctt 1315
Thr Leu Gln Leu Leu Ser Ser Gly Ala Thr Thr Ala Glu Thr Gln Leu
390 395 400 405
gcc cag ctg ccg gat gaa acc gcg ttc gtg ctc att gac cca tca tct 1363
Ala Gln Leu Pro Asp Glu Thr Ala Phe Val Leu Ile Asp Pro Ser Ser
410 415 420
ggt gct gtt act gac ctg ttc ttt atc caa agt ctc aac act gat ctg 1411
Gly Ala Val Thr Asp Leu Phe Phe Ile Gln Ser Leu Asn Thr Asp Leu
425 430 435
ccg gca cca aca acc cag att aat tct att gct gtt gat gag cgc gac 1459
Pro Ala Pro Thr Thr Gln Ile Asn Ser Ile Ala Val Asp Glu Arg Asp
440 445 450
ccc aac atc atc tac gcc act ttt agc ggc gat gac cac ctg tac caa 1507
Pro Asn Ile Ile Tyr Ala Thr Phe Ser Gly Asp Asp His Leu Tyr Gln
455 460 465
atg atg ctg ggt tagttacaca actaactaac caggcaccaa 1549
Met Met Leu Gly
470
<210>202
<211>473
<212>PRT
<213>谷氨酸棒状杆菌
<400>202
Met Arg Tyr Thr Pro Arg Thr His Arg Ala Leu Gly Leu Ala Ala Leu
1 5 10 15
Thr Ala Thr Ala Leu Leu Leu Ser Ser Cys Ser Thr Thr Ala Asp Leu
20 25 30
Lau Gly Ala Asp Ser Val Ala Gln Ala Val Thr Asp Gly Gly Ser Ala
35 40 45
Leu Asp Pro Thr Ala Ala His Val Leu Thr Ile Thr Asn Ala Thr Ala
50 55 60
Met Thr Leu Ala Glu Leu Pro Glu Asn Thr Asp Pro Asp Ala Ala Asn
65 70 75 80
Asn Thr Glu Pro Thr Met Ala Gln Ser Ala Leu Lys Arg Ile Tyr Gly
85 90 95
Leu Glu Ile Ala Ala Asp Gly Thr Leu Ile Ala Val Ala Ala Pro Leu
100 105 110
Thr Ser Arg Asn Ser Gly Ser Glu Gln Pro Thr Val Ala Ala Met Ala
115 120 125
Asp Pro Glu Asn Asn Ala Glu Leu Thr Thr Ala His Ile Leu Glu Ile
130 135 140
Asp Pro Ala Thr Gly Thr Ala Thr Pro Arg Gln Asp Ile Ala Leu Thr
145 150 155 160
Glu Asp Phe Gln Thr Met Ala Ser Ser Phe Ser Gln Ile Arg Asn Gly
165 170 175
Trp Gly Thr Thr Gln Leu Ile Gly Gln His Pro Asp Asp Pro Lys Gln
180 185 190
Pro Ile Arg Ser Ala Asp Thr Trp Thr Val Thr Gly Ser Thr Gln Ile
195 200 205
Thr Gly Phe Asn Thr Asn Thr Pro Thr Gly Thr Glu Asp Ala Ala Tyr
210 215 220
Thr Met Pro Ala Thr Glu Pro Ala Val Gly Leu Cys Ala Leu Glu Ser
225 230 235 240
Gly Ser Asp Ala Pro Leu Asp Glu His Arg Glu Leu Ser Thr Ser Ala
245 250 255
Leu Arg Thr Ala Ser Val Leu Ser Ser Ser Gly Ser Ala Thr Leu Lys
260 265 270
Leu His Asp Pro Met Val Met Ser Ala Thr Gly Ile Val Gln Ala Arg
275 280 285
Ala Tyr Val Asp Gly Glu Val Ile Asn Gln His Glu Ile Gly Asp Leu
290 295 300
Arg Glu Gln Leu Gly Ile Asn Ile Glu Glu Ser Glu Ala Ala Gln Ala
305 310 315 320
Glu Ser Asp Ala Gln Ala Ala Thr Asp His Pro Leu Ala Ala Leu Gly
325 330 335
Leu Ser Asn Pro Thr Ser Ser Ala Leu Val Pro Gly Leu Ala Glu Leu
340 345 350
Asp Cys Leu Ser Ala Asp Gln Ala Ala Thr Trp His Asn Arg Asp Thr
355 360 365
Ser Ile Gly Thr Gly Lys Pro Ser Val Leu Ala Val Ile Asn Ala Glu
370 375 380
Met Ala Asp Asp Phe Thr Leu Gln Leu Leu Ser Ser Gly Ala Thr Thr
385 390 395 400
Ala Glu Thr Gln Leu Ala Gln Leu Pro Asp Glu Thr Ala Phe Val Leu
405 410 415
Ile Asp Pro Ser Ser Gly Ala Val Thr Asp Leu Phe Phe Ile Gln Ser
420 425 430
Leu Asn Thr Asp Leu Pro Ala Pro Thr Thr Gln Ile Asn Ser Ile Ala
435 440 445
Val Asp Glu Arg Asp Pro Asn Ile Ile Tyr Ala Thr Phe Ser Gly Asp
450 455 460
Asp His Leu Tyr Gln Met Met Leu Gly
465 470
<210>203
<211>772
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(742)
<22 3>RXA01789
<400>203
tgcatcccaa caccacaaca ccgcccctag tgccataaat gctaggggtg tttttttcat 60
acccacaaca ccgttcttct ctagaaagag actcgatact atg cct cat aac ccc 115
Met Pro His Asn Pro
1 5
atc gaa ctt cac act aat gat gta ctc gat gct ctc gac att gac gcc 163
Ile Glu Leu His Thr Asn Asp Val Leu Asp Ala Leu Asp Ile Asp Ala
10 15 20
atc tgc gac gat gtg ttt ctc tac acc gac ttc gag cac acc cca ggt 211
Ile Cys Asp Asp Val Phe Leu Tyr Thr Asp Phe Glu His Thr Pro Gly
25 30 35
cag ctt gat cgc ttc gag caa ctc gcc ttc aca aaa atc tac gac atg 259
Gln Leu Asp Arg Phe Glu Gln Leu Ala Phe Thr Lys Ile Tyr Asp Met
40 45 50
ctg gaa aca gct gct gaa aaa ttc cca gat gta gca att aat gac act 307
Leu Glu Thr Ala Ala Glu Lys Phe Pro Asp Val Ala Ile Asn Asp Thr
55 60 65
tta agc act ggc aac cac gct gct gaa cag tac ttt ctt gcc aat ccc 355
Leu Ser Thr Gly Asn His Ala Ala Glu Gln Tyr Phe Leu Ala Asn Pro
70 75 80 85
ggc aac atc atc gtg ctg acc agc ttc gcg ctc aat cag acc gat ctc 403
Gly Asn Ile Ile Val Leu Thr Ser Phe Ala Leu Asn Gln Thr Asp Leu
90 95 100
cgc gac ctc ata atc tca ccg tgc att aag tac aca gca cat gca cga 451
Arg Asp Leu Ile Ile Ser Pro Cys Ile Lys Tyr Thr Ala His Ala Arg
105 110 115
gca ctc atg cgt gcg gtg aca cga aca ttg tgc act gca aac aac cct 499
Ala Leu Met Arg Ala Val Thr Arg Thr Leu Cys Thr Ala Asn Asn Pro
120 125 130
gta gaa cgc gcg act atc ttc cct gtg tct gtc gct aat gcc tta agc 547
Val Glu Arg Ala Thr Ile Phe Pro Val Ser Val Ala Asn Ala Leu Ser
135 140 145
atc gaa gcg ctg tgt tcc gaa tat cat gcg ttc cgc acc aag cag gtg 595
Ile Glu Ala Leu Cys Ser Glu Tyr His Ala Phe Arg Thr Lys Gln Val
150 155 160 165
ctc aac act gct gca ctc atc aat cct gat aac act ctt ata ccc atg 643
Leu Asn Thr Ala Ala Leu Ile Asn Pro Asp Asn Thr Leu Ile Pro Met
170 175 180
ttg ttg agc aaa gcg tat gag gca tac gcc tgg cac aag ggt gtc gaa 691
Leu Leu Ser Lys Ala Tyr Glu Ala Tyr Ala Trp His Lys Gly Val Glu
185 190 195
tcc gcg caa cga ggt agc aac cta gct cgt gag tac tac gca gga ctt 739
Ser Ala Gln Arg Gly Ser Asn Leu Ala Arg Glu Tyr Tyr Ala Gly Leu
200 205 210
atc tgaaaattca ctactcaact ctggccccaa 772
Ile
<210>204
<211>214
<212>PRT
<213>谷氨酸棒状杆菌
<400>204
Met Pro His Asn Pro Ile Glu Leu His Thr Asn Asp Val Leu Asp Ala
1 5 10 15
Leu Asp Ile Asp Ala Ile Cys Asp Asp Val Phe Leu Tyr Thr Asp Phe
20 25 30
Glu His Thr Pro Gly Gln Leu Asp Arg Phe Glu Gln Leu Ala Phe Thr
35 40 45
Lys Ile Tyr Asp Met Leu Glu Thr Ala Ala Glu Lys Phe Pro Asp Val
50 55 60
Ala Ile Asn Asp Thr Leu Ser Thr Gly Asn His Ala Ala Glu Gln Tyr
65 70 75 80
Phe Leu Ala Asn Pro Gly Asn Ile Ile Val Leu Thr Ser Phe Ala Leu
85 90 95
Asn Gln Thr Asp Leu Arg Asp Leu Ile Ile Ser Pro Cys Ile Lys Tyr
100 105 110
Thr Ala His Ala Arg Ala Leu Met Arg Ala Val Thr Arg Thr Leu Cys
115 120 125
Thr Ala Asn Asn Pro Val Glu Arg Ala Thr Ile Phe Pro Val Ser Val
130 135 140
Ala Asn Ala Leu Ser Ile Glu Ala Leu Cys Ser Glu Tyr His Ala Phe
145 150 155 160
Arg Thr Lys Gln Val Leu Asn Thr Ala Ala Leu Ile Asn Pro Asp Asn
165 170 175
Thr Leu Ile Pro Met Leu Leu Ser Lys Ala Tyr Glu Ala Tyr Ala Trp
180 185 190
His Lys Gly Val Glu Ser Ala Gln Arg Gly Ser Asn Leu Ala Arg Glu
195 200 205
Tyr Tyr Ala Gly Leu Ile
210
<210>205
<211>493
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(463)
<223>RXA01791
<400>205
ccgcctgtgc aactaaccct gttgtgcagg cggttttctc tatctacacc aacaactcca 60
caacactatc cggtccaaat ctagaaaaga gacaaccccc atg acc tcc ccc acc 115
Met Thr Ser Pro Thr
1 5
acg tta aag ccc tgg tct att cgc gga ccc atc gac ggt gaa tac acc 163
Thr Leu Lys Pro Trp Ser Ile Arg Gly Pro Ile Asp Gly Glu Tyr Thr
10 15 20
atg ctt gag gca tat ccg ttt cag tat gta gca aat act cgc ccg gcc 211
Met Leu Glu Ala Tyr Pro Phe Gln Tyr Val Ala Asn Thr Arg Pro Ala
25 30 35
atc atg ctc tac gca ctc gat gaa aac ggt gta ccg gaa caa tat gcc 259
Ile Met Leu Tyr Ala Leu Asp Glu Asn Gly Val Pro Glu Gln Tyr Ala
40 45 50
gac ctc acc atc aac ctt gcc gat gtg gag ctc aag caa ctt aac cac 307
Asp Leu Thr Ile Asn Leu Ala Asp Val Glu Leu Lys Gln Leu Asn His
55 60 65
gtc atc att aac cca gat ctg cac gct gat gtt acc gaa ctg tgc att 355
Val Ile Ile Asn Pro Asp Leu His Ala Asp Val Thr Glu Leu Cys Ile
70 75 80 85
agc gcc ggc ctg ctg cga cct ggt gtt ctg ggc cag cac caa gtc ggt 403
Ser Ala Gly Leu Leu Arg Pro Gly Val Leu Gly Gln His Gln Val Gly
90 95 100
agc acc acc gcc aag gtc tac cga ctc act gag cgt gct gat ggc tgg 451
Ser Thr Thr Ala Lys Val Tyr Arg Leu Thr Glu Arg Ala Asp Gly Trp
105 110 115
cta cag ctg ttt tagcagctac ctgcgcctaa tccccccacc 493
Leu Gln Leu Phe
120
<210>206
<211>121
<212>PRT
<213>谷氨酸棒状杆菌
<400>206
Met Thr Ser Pro Thr Thr Leu Lys Pro Trp Ser Ile Arg Gly Pro Ile
1 5 10 15
Asp Gly Glu Tyr Thr Met Leu Glu Ala Tyr Pro Phe Gln Tyr Val Ala
20 25 30
Asn Thr Arg Pro Ala Ile Met Leu Tyr Ala Leu Asp Glu Asn Gly Val
35 40 45
Pro Glu Gln Tyr Ala Asp Leu Thr Ile Asn Leu Ala Asp Val Glu Leu
50 55 60
Lys Gln Leu Asn His Val Ile Ile Asn Pro Asp Leu His Ala Asp Val
65 70 75 80
Thr Glu Leu Cys Ile Ser Ala Gly Leu Leu Arg Pro Gly Val Leu Gly
85 90 95
Gln His Gln Val Gly Ser Thr Thr Ala Lys Val Tyr Arg Leu Thr Glu
100 105 110
Arg Ala Asp Gly Trp Leu Gln Leu Phe
115 120
<210>207
<211>1306
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1276)
<223>RXA01809
<400>207
acaacacgac agttgtaact agtggtttca acaccattat cggtatgact cgtggcgact 60
cgtatctctc tgatgatctc gatgacctgg gttaaacccg atg aac gag cag gaa 115
Met Asn Glu Gln Glu
1 5
cga gaa gcc tta gag gat gct gcc ctt gag gaa gct gcc tta gcc gat 163
Arg Glu Ala Leu Glu Asp Ala Ala Leu Glu Glu Ala Ala Leu Ala Asp
10 15 20
gaa tta gct gca tta gag gct gaa gct ggc gta caa ggg tca gtc gag 211
Glu Leu Ala Ala Leu Glu Ala Glu Ala Gly Val Gln Gly Ser Val Glu
25 30 35
cct tat gac tat gca gca gac ctt gat gat gag gac gag ttt gat gag 259
Pro Tyr Asp Tyr Ala Ala Asp Leu Asp Asp Glu Asp Glu Phe Asp Glu
40 45 50
gac cct ttt gct cag gat gaa ccc cgt gac gct ggt ccg cta ggt gag 307
Asp Pro Phe Ala Gln Asp Glu Pro Arg Asp Ala Gly Pro Leu Gly Glu
55 60 65
ttg agc agt gat aac cat gtc tct gag gct gtt gct gaa gac act ggg 355
Leu Ser Ser Asp Asn His Val Ser Glu Ala Val Ala Glu Asp Thr Gly
70 75 80 85
aca agt aca gaa gag tct gca caa gag ggc agt cac gaa gag tcg gta 403
Thr Ser Thr Glu Glu Ser Ala Gln Glu Gly Ser His Glu Glu Ser Val
90 95 100
gac aat ccc cgt gat ttc acc ggc act gcg aca gcg gtg cga tct ttt 451
Asp Asn Pro Arg Asp Phe Thr Gly Thr Ala Thr Ala Val Arg Ser Phe
105 110 115
cga ccc aga ctt ccc gta ccc aat gca ctg cga cca gga cca ccc atc 499
Arg Pro Arg Leu Pro Val Pro Asn Ala Leu Arg Pro Gly Pro Pro Ile
120 125 130
cga aca caa ccg gca gtg aac acc gac ata gat gat ggc ggg caa gag 547
Arg Thr Gln Pro Ala Val Asn Thr Asp Ile Asp Asp Gly Gly Gln Glu
135 140 145
aat aca gct ggc gcg act gcc gca gat gtg ggg gcg gga gtg ttt ttt 595
Asn Thr Ala Gly Ala Thr Ala Ala Asp Val Gly Ala Gly Val Phe Phe
150 155 160 165
ggt acg cat ttt gag ctg gct ttt ggg cag gtt gat gtt cac cgc cac 643
Gly Thr His Phe Glu Leu Ala Phe Gly Gln Val Asp Val His Arg His
170 175 180
ata agg cat gag cag gct atg cga gat gga cac cag gat ctc agc gtc 691
Ile Arg His Glu Gln Ala Met Arg Asp Gly His Gln Asp Leu Ser Val
185 190 195
ttt gtc ggt gct gat ggg gcg atc atg ctg gaa gaa ctt aaa aat cgt 739
Phe Val Gly Ala Asp Gly Ala Ile Met Leu Glu Glu Leu Lys Asn Arg
200 205 210
tac cag cgc cca ccg gag aac gag ccc cat gat gat gat gtc acc gaa 787
Tyr Gln Arg Pro Pro Glu Asn Glu Pro His Asp Asp Asp Val Thr Glu
215 220 225
atg ata aag acc acc gag gaa aca gag aag gag aac gta gag atg gat 835
Met Ile Lys Thr Thr Glu Glu Thr Glu Lys Glu Asn Val Glu Met Asp
230 235 240 245
cag gat ata gct gta gta gac aac gtt gat gaa cag gtt gtg gtg aca 883
Gln Asp Ile Ala Val Val Asp Asn Val Asp Glu Gln Val Val Val Thr
250 255 260
cct gct cat gac gct gac agc gta gcg gtt gtt gct gaa cag gtc gtt 931
Pro Ala His Asp Ala Asp Ser Val Ala Val Val Ala Glu Gln Val Val
265 270 275
gcg tca gaa cca acg cca gag ccg gta ccc aag gtt gag cac gta gag 979
Ala Ser Glu Pro Thr Pro Glu Pro Val Pro Lys Val Glu His Val Glu
280 285 290
atg agt gtc gat cta gcg ggt gag act att aca ccg att gat cag gca 1027
Met Ser Val Asp Leu Ala Gly Glu Thr Ile Thr Pro Ile Asp Gln Ala
295 300 305
att cgg tct ttt atg cag ctc aac ggt att gag cac agc gtg gtg ttg 1075
Ile Arg Ser Phe Met Gln Leu Asn Gly Ile Glu His Ser Val Val Leu
310 315 320 325
cga cgc ttg ggg ctg atg act cag gtg gca cat aat aag gaa ctt gat 1123
Arg Arg Leu Gly Leu Met Thr Gln Val Ala His Asn Lys Glu Leu Asp
330 335 340
gag gtc tac gct aag gca ctt gcc gag ggt gaa tcc cat gct cag cag 1171
Glu Val Tyr Ala Lys Ala Leu Ala Glu Gly Glu Ser His Ala Gln Gln
345 350 355
cag cat gaa tta gaa gca gaa aat gaa cgg ctt aaa aaa gaa gtt gat 1219
Gln His Glu Leu Glu Ala Glu Asn Glu Arg Leu Lys Lys Glu Val Asp
360 365 370
gcg ctc gct gca gag ctt agt gct gcg ttg atg ggc gag ggt ggt ggt 1267
Ala Leu Ala Ala Glu Leu Ser Ala Ala Leu Met Gly Glu Gly Gly Gly
375 380 385
gat cat gac taaggccgtg acaccaattg tggtgaccga 1306
Asp His Asp
390
<210>208
<211>392
<212>PRT
<213>谷氨酸棒状杆菌
<400>208
Met Asn Glu Gln Glu Arg Glu Ala Leu Glu Asp Ala Ala Leu Glu Glu
1 5 10 15
Ala Ala Leu Ala Asp Glu Leu Ala Ala Leu Glu Ala Glu Ala Gly Val
20 25 30
Gln Gly Ser Val Glu Pro Tyr Asp Tyr Ala Ala Asp Leu Asp Asp Glu
35 40 45
Asp Glu Phe Asp Glu Asp Pro Phe Ala Gln Asp Glu Pro Arg Asp Ala
50 55 60
Gly Pro Leu Gly Glu Leu Ser Ser Asp Asn His Val Ser Glu Ala Val
65 70 75 80
Ala Glu Asp Thr Gly Thr Ser Thr Glu Glu Ser Ala Gln Glu Gly Ser
85 90 95
His Glu Glu Ser Val Asp Asn Pro Arg Asp Phe Thr Gly Thr Ala Thr
100 105 110
Ala Val Arg Ser Phe Arg Pro Arg Leu Pro Val Pro Asn Ala Leu Arg
115 120 125
Pro Gly Pro Pro Ile Arg Thr Gln Pro Ala Val Asn Thr Asp Ile Asp
130 135 140
Asp Gly Gly Gln Glu Asn Thr Ala Gly Ala Thr Ala Ala Asp Val Gly
145 150 155 160
Ala Gly Val Phe Phe Gly Thr His Phe Glu Leu Ala Phe Gly Gln Val
165 170 175
Asp Val His Arg His Ile Arg His Glu Gln Ala Met Arg Asp Gly His
180 185 190
Gln Asp Leu Ser Val Phe Val Gly Ala Asp Gly Ala Ile Met Leu Glu
195 200 205
Glu Leu Lys Asn Arg Tyr Gln Arg Pro Pro Glu Asn Glu Pro His Asp
210 215 220
Asp Asp Val Thr Glu Met Ile Lys Thr Thr Glu Glu Thr Glu Lys Glu
225 230 235 240
Asn Val Glu Met Asp Gln Asp Ile Ala Val Val Asp Asn Val Asp Glu
245 250 255
Gln Val Val Val Thr Pro Ala His Asp Ala Asp Ser Val Ala Val Val
260 265 270
Ala Glu Gln Val Val Ala Ser Glu Pro Thr Pro Glu Pro Val Pro Lys
275 280 285
Val Glu His Val Glu Met Ser Val Asp Leu Ala Gly Glu Thr Ile Thr
290 295 300
Pro Ile Asp Gln Ala Ile Arg Ser Phe Met Gln Leu Asn Gly Ile Glu
305 310 315 320
His Ser Val Val Leu Arg Arg Leu Gly Leu Met Thr Gln Val Ala His
325 330 335
Asn Lys Glu Leu Asp Glu Val Tyr Ala Lys Ala Leu Ala Glu Gly Glu
340 345 350
Ser His Ala Gln Gln Gln His Glu Leu Glu Ala Glu Asn Glu Arg Leu
355 360 365
Lys Lys Glu Val Asp Ala Leu Ala Ala Glu Leu Ser Ala Ala Leu Met
370 375 380
Gly Glu Gly Gly Gly Asp His Asp
385 390
<210>209
<211>400
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(370)
<223>RXA01825
<400>209
gtcaaggtag ctggcccggc aactgatacgttaagctcaa acaagataag taccagttgc 60
tggggttttt ccaagacaat aaattatgaa ggtgtgaaca atg cca aag gca aga 115
Met Pro Lys Ala Arg
1 5
gta act aaa aac gag acc gca ccg gtt tca agc aac cca agc gca aac 163
Val Thr Lys Asn Glu Thr Ala Pro Val Ser Ser Asn Pro Ser Ala Asn
10 15 20
cgc acc ccg gtt aag atc aat tcc gcc gga acc cca atg tgg tac aag 211
Arg Thr Pro Val Lys Ile Asn Ser Ala Gly Thr Pro Met Trp Tyr Lys
25 30 35
gtc atc atg ttt gcc ttc atg atc gtc ggc cta gcc tgg ttg atc att 259
Val Ile Met Phe Ala Phe Met Ile Val Gly Leu Ala Trp Leu Ile Ile
40 45 50
aac tac ctc gtg ggc cca cag atc cca ttc atg gct gat ctt ggt gca 307
Asn Tyr Leu Val Gly Pro Gln Ile Pro Phe Met Ala Asp Leu Gly Ala
55 60 65
tgg aac tat ggc atc ggc ttc ggt ctg atg atc atc ggc cta ctc atg 355
Trp Asn Tyr Gly Ile Gly Phe Gly Leu Met Ile Ile Gly Leu Leu Met
70 75 80 85
acc atg ggt tgg cgt taatccttca aaaaagtgac tgccgcagca 400
Thr Met Gly Trp Arg
90
<210>210
<211>90
<212>PRT
<213>谷氨酸棒状杆菌
<400>210
Met Pro Lys Ala Arg Val Thr Lys Asn Glu Thr Ala Pro Val Ser Ser
1 5 10 15
Asn Pro Ser Ala Asn Arg Thr Pro Val Lys Ile Asn Ser Ala Gly Thr
20 25 30
Pro Met Trp Tyr Lys Val Ile Met Phe Ala Phe Met Ile Val Gly Leu
35 40 45
Ala Trp Leu Ile Ile Asn Tyr Leu Val Gly Pro Gln Ile Pro Phe Met
50 55 60
Ala Asp Leu Gly Ala Trp Asr Tyr Gly Ile Gly Phe Gly Leu Met Ile
65 70 75 80
Ile Gly Leu Leu Met Thr Met Gly Trp Arg
85 90
<210>211
<211>1114
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1084)
<223>RXA01842
<400>211
ggcctgcgcg atagatcccc caatgtcggt ggggtccttc gtgatcactt ctttcactct 60
ttgatccacg tcatggtctc gcggtgatca tcttcctacc gtg aca ggc aag gcc 115
Val Thr Gly Lys Ala
1 5
gca aac ggc cgt gac cac agg aaa gaa att cac gag gag agg aag cac 163
Ala Asn Gly Arg Asp His Arg Lys Glu Ile His Glu Glu Arg Lys His
10 15 20
acg atg tcg aag gta tac gtg tcc aac gag tac ggc ggc ccg gaa aac 211
Thr Met Ser Lys Val Tyr Val Ser Asn Glu Tyr Gly Gly Pro Glu Asn
25 30 35
cag gaa ctg atc acc cgc aac acc ccc cag cca ggc ccg gga gaa ctc 259
Gln Glu Leu Ile Thr Arg Asn Thr Pro Gln Pro Gly Pro Gly Glu Leu
40 45 50
ggg gtc aag gtc cac gcg gcc ggg gtc aac ccg ctt gat tgg aag gtc 307
Gly Val Lys Val His Ala Ala Gly Val Asn Pro Leu Asp Trp Lys Val
55 60 65
cgt tcc ggg gtt gcc gga acc ccg cga gag ctt ccg gca ccc ctg ggc 355
Arg Ser Gly Val Ala Gly Thr Pro Arg Glu Leu Pro Ala Pro Leu Gly
70 75 80 85
gag gag gcc tcc ggg atc gtc acc gcc gtt gga gac ggt gtg gag ggc 403
Glu Glu Ala Ser Gly Ile Val Thr Ala Val Gly Asp Gly Val Glu Gly
90 95 100
ttc gcg gcc ggg gat ccg gtg ctc ggc ctg gtg gcc ccc ggc gtc ggc 451
Phe Ala Ala Gly Asp Pro Val Leu Gly Leu Val Ala Pro Gly Val Gly
105 110 1 15
gga tat gcc gag gac acc ctg ctg gtg gca gag agt acc gtg cta aag 499
Gly Tyr Ala Glu Asp Thr Leu Leu Val Ala Glu Ser Thr Val Leu Lys
120 125 130
ccg gag gag atc tcg ttc acc gac gcc gcc gcg atc ccg gtc gct ggg 547
Pro Glu Glu Ile Ser Phe Thr Asp Ala Ala Ala Ile Pro Val Ala Gly
135 140 145
gcg agc gcc tac gcc ggc act cac cag gtc gag ctt gaa cca ggc cag 595
Ala Ser Ala Tyr Ala Gly Thr His Gln Val Glu Leu Glu Pro Gly Gln
150 155 160 165
tcg ttg ctg atc aat ggg gcc ggt ggt ggg gtc ggg ctg atg gcc gcg 643
Ser Leu Leu Ile Asn Gly Ala GLy Gly Gly Val Gly Leu Met Ala Ala
170 175 180
cag atc gga cgg gtc cac aag ttc cag gtc gtc ggc gtt gac cac gag 691
Gln Ile Gly Arg Val His Lys Phe Gln Val Val Gly Val Asp His Glu
185 190 195
gac aag cgc gag ctc atc gaa tcc acc ggt gct atc ttc gtc gcc acc 739
Asp Lys Arg Glu Leu Ile Glu Ser Thr Gly Ala Ile Phe Val Ala Thr
200 205 210
ggc gac gcc gtc gcg gag cag gtg cgt gcg ctg ctc cct gac ggt gtg 787
Gly Asp Ala Val Ala Glu Gln Val Arg Ala Leu Leu Pro Asp Gly Val
215 220 225
gac gta gtc ttc gac cta gtc ggc ggg gag gcg ttg cgg gtg gtt gct 835
Asp Val Val Phe Asp Leu Val Gly Gly Glu Ala Leu Arg Val Val Ala
230 235 240 245
ccc tta gcg aag aat ccg gcg cac gtg atc tcg gcg gct gat gct gcc 883
Pro Leu Ala Lys Asn Pro Ala His Val Ile Ser Ala Ala Asp Ala Ala
250 255 260
acc gtg gga gaa ctc ggt gga cag gtg ctg cgc cgc acc ccg gaa atg 931
Thr Val Gly Glu Leu Gly Gly Gln Val Leu Arg Arg Thr Pro Glu Met
265 270 275
gtc gga cag atc acc ggg gtg gtc cag tac ggg ctg gtc gac ccg aag 979
Val Gly Gln Ile Thr Gly Val Val Gln Tyr Gly Leu Val Asp Pro Lys
280 285 290
gtc gat acg acc tac ccg ctg gaa cag gcc ggt aag gcc ctg gcc cac 1027
Val Asp Thr Thr Tyr Pro Leu Glu Gln Ala Gly Lys Ala Leu Ala His
295 300 305
gtt gag cag ggc cac gcc cgc ggc aag atc gtc ctc gag ctc atc acc 1075
Val Glu Gln Gly His Ala Arg Gly Lys Ile Val Leu Glu Leu Ile Thr
310 315 320 325
tcc cag gac taaccagaca acgcggtgac ctccctcagg 1114
Ser Gln Asp
<210>212
<211>328
<212>PRT
<213>谷氨酸棒状杆菌
<400>212
Val Thr Gly Lys Ala Ala Asn Gly Arg Asp His Arg Lys Glu Ile His
1 5 10 15
Glu Glu Arg Lys His Thr Met Ser Lys Val Tyr Val Ser Asn Glu Tyr
20 25 30
Gly Gly Pro Glu Asn Gln Glu Leu Ile Thr Arg Asn Thr Pro Gln Pro
35 40 45
Gly Pro Gly Glu Leu Gly Val Lys Val His Ala Ala Gly Val Asn Pro
50 55 60
Leu Asp Trp Lys Val Arg Ser Gly Val Ala Gly Thr Pro Arg Glu Leu
65 70 75 80
Pro Ala Pro Leu Gly Glu Glu Ala Ser Gly Ile Val Thr Ala Val Gly
85 90 95
Asp Gly Val Glu Gly Phe Ala Ala Gly Asp Pro Val Leu Gly Leu Val
100 105 110
Ala Pro Gly Val Gly Gly Tyr Ala Glu Asp Thr Leu Leu Val Ala Glu
115 120 125
Ser Thr Val Leu Lys Pro Glu Glu Ile Ser Phe Thr Asp Ala Ala Ala
130 135 140
Ile Pro Val Ala Gly Ala Ser Ala Tyr Ala Gly Thr His Gln Val Glu
145 150 155 160
Leu Glu Pro Gly Gln Ser Leu Leu Ile Asn Gly Ala Gly Gly Gly Val
165 170 175
Gly Leu Met Ala Ala Gln Ile Gly Arg Val His Lys Phe Gln Val Val
180 185 190
Gly Val Asp His Glu Asp Lys Arg Glu Leu Ile Glu Ser Thr Gly Ala
195 200 205
Ile Phe Val Ala Thr Gly Asp Ala Val Ala Glu Gln Val Arg Ala Leu
210 215 220
Leu Pro Asp Gly Val Asp Val Val Phe Asp Leu Val Gly Gly Glu Ala
225 230 235 240
Leu Arg Val Val Ala Pro Leu Ala Lys Asn Pro Ala His Val Ile Ser
245 250 255
Ala Ala Asp Ala Ala Thr Val Gly Glu Leu Gly Gly Gln Val Leu Arg
260 265 270
Arg Thr Pro Glu Met Val Gly Gln Ile Thr Gly Val Val Gln Tyr Gly
275 280 285
Leu Val Asp Pro Lys Val Asp Thr Thr Tyr Pro Leu Glu Gln Ala Gly
290 295 300
Lys Ala Leu Ala His Val Glu Gln Gly His Ala Arg Gly Lys Ile Val
305 310 315 320
Leu Glu Leu Ile Thr Ser Gln Asp
325
<210>213
<211>916
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(886)
<223>RXA01899
<400>213
cctcgcagca cttcgcgcag gcgaatccac agtggccgca accttgatca aagaacacat 60
cgaaggctac tacgaagaaa ccgctgccgc cgaggcctaa atg tcc cgc act ctg 115
Met Ser Arg Thr Leu
1 5
tgg gcg gtt tca gat cta cac gtc acc ttc gcc caa aac caa aac acc 163
Trp Ala Val Ser Asp Leu His Val Thr Phe Ala Gln Asn Gln Asn Thr
10 15 20
gtt gat gcc ctc atg ccg cag gac ccc ggc gac tgg ctg atc gtc gct 211
Val Asp Ala Leu Met Pro Gln Asp Pro Gly Asp Trp Leu Ile Val Ala
25 30 35
ggc gat gta gca gag aaa atc ccc gat gtg gta cgt acc tta tcc gcg 259
Gly Asp Val Ala Glu Lys Ile Pro Asp Val Val Arg Thr Leu Ser Ala
40 45 50
ctg gtc aaa cgc ttt gac acc gtg att tgg gtg ccg ggc aac cac gaa 307
Leu Val Lys Arg Phe Asp Thr Val Ile Trp Val Pro Gly Asn His Glu
55 60 65
ctc ttc aac cgg aaa aca gac cgc gtc aac ggc aaa gcc cgc tac cga 355
Leu Phe Asn Arg Lys Thr Asp Arg Val Asn Gly Lys Ala Arg Tyr Arg
70 75 80 85
gca tta gtc gga caa ctc cga gcc atc ggc gtg atc acc ccc gaa gat 403
Ala Leu Val Gly Gln Leu Arg Ala Ile Gly Val Ile Thr Pro Glu Asp
90 95 100
ccc tat ccg atc ttt ggt ggc gtc acc atc tgc cca ctt ttc aca ctt 451
Pro Tyr Pro Ile Phe Gly Gly Val Thr Ile Cys Pro Leu Phe Thr Leu
105 110 115
tac gat tac tct ttc cgt ccc ctc ggc ctc acc gcg aaa caa gcc ctc 499
Tyr Asp Tyr Ser Phe Arg Pro Leu Gly Leu Thr Ala Lys Gln Ala Leu
120 125 130
gcc caa gca aaa ata aag cta gac gac gaa cta gcc atc gcc ccc tac 547
Ala Gln Ala Lys Ile Lys Leu Asp Asp Glu Leu Ala Ile Ala Pro Tyr
135 140 145
gta gac atc ccc gcc tgg tgc gcc gaa cga gtc acc tac aca gaa gac 595
Val Asp Ile Pro Ala Trp Cys Ala Glu Arg Val Thr Tyr Thr Glu Asp
150 155 160 165
cgc cta aaa gcc acc aaa ggc cca aaa gtc ctg gtc aat cac tgg ccg 643
Arg Leu Lys Ala Thr Lys Gly Pro Lys Val Leu Val Asn His Trp Pro
170 175 180
ctg gtc att gag ccc acc cac cgg ctc ttc caa aaa gac atc gcg ctg 691
Leu Val Ile Glu Pro Thr His Arg Leu Phe Gln Lys Asp Ile Ala Leu
185 190 195
tgg tgt gga acc acc gcc acc agg gat tgg gcc gta cga ttc aac gct 739
Trp Cys Gly Thr Thr Ala Thr Arg Asp Trp Ala Val Arg Phe Asn Ala
200 205 210
ctc atg gcc att cac ggt cac cta cat att cct gcc gaa acc cgc gtt 787
Leu Met Ala Ile His Gly His Leu His Ile Pro Ala Glu Thr Arg Val
215 220 225
gat ggg gta agc cac gtg gag gtt tct ttg ggt tac ccc ttt gaa aaa 835
Asp Gly Val Ser His Val Glu Val Ser Leu Gly Tyr Pro Phe Glu Lys
230 235 240 245
cac cca cct cac atg aag cgt ccg tgg ccg ttt ccg gtc atg cag att 883
His Pro Pro His Met Lys Arg Pro Trp Pro Phe Pro Val Met Gln Ile
250 255 260
aac taactctgtt gcttaaatgg gggtaattgg 916
Asn
<210>214
<211>262
<212>PRT
<213>谷氨酸棒状杆菌
<400>214
Met Ser Arg Thr Leu Trp Ala Val Ser Asp Leu His Val Thr Phe Ala
1 5 10 15
Gln Asn Gln Asn Thr Val Asp Ala Leu Met Pro Gln Asp Pro Gly Asp
20 25 30
Trp Leu Ile Val Ala Gly Asp Val Ala Glu Lys Ile Pro Asp Val Val
35 40 45
Arg Thr Leu Ser Ala Leu Val Lys Arg Phe Asp Thr Val Ile Trp Val
50 55 60
Pro Gly Asn His Glu Leu Phe Asn Arg Lys Thr Asp Arg Val Asn Gly
65 70 75 80
Lys Ala Arg Tyr Arg Ala Leu Val Gly Gln Leu Arg Ala Ile Gly Val
85 90 95
Ile Thr Pro Glu Asp Pro Tyr Pro Ile Phe Gly Gly Val Thr Ile Cys
100 105 110
Pro Leu Phe Thr Leu Tyr Asp Tyr Ser Phe Arg Pro Leu Gly Leu Thr
115 120 125
Ala Lys Gln Ala Leu Ala Gln Ala Lys Ile Lys Leu Asp Asp Glu Leu
130 135 140
Ala Ile Ala Pro Tyr Val Asp Ile Pro Ala Trp Cys Ala Glu Arg Val
145 150 155 160
Thr Tyr Thr Glu Asp Arg Leu Lys Ala Thr Lys Gly Pro Lys Val Leu
165 170 175
Val Asn His Trp Pro Leu Val Ile Glu Pro Thr His Arg Leu Phe Gln
180 185 190
Lys Asp Ile Ala Leu Trp Cys Gly Thr Thr Ala Thr Arg Asp Trp Ala
195 200 205
Val Arg Phe Asn Ala Leu Met Ala Ile His Gly His Leu His Ile Pro
210 215 220
Ala Glu Thr Arg Val Asp Gly Val Ser His Val Glu Val Ser Leu Gly
225 230 235 240
Tyr Pro Phe Glu Lys His Pro Pro His Met Lys Arg Pro Trp Pro Phe
245 250 255
Pro Val Met Gln Ile Asn
260
<210>215
<211>661
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(631)
<223>RXA01905
<400>215
aaatgaaagt cacaaacttt gttggggttt ctttaaagta aaatacagaa gtttctctgg 60
caaaaaacct cactactttc tacagaaagg cgtcttcctc atg aag gct tct cag 115
Met Lys Ala Ser Gln
1 5
acc ctc acc cgc aac act cgt gca cgc aag ctc ggc atg ggt att ctc 163
Thr Leu Thr Arg Asn Thr Arg Ala Arg Lys Leu Gly Met Gly Ile Leu
10 15 20
gca gca act att atg gct acc ggt gca ctt gct ggc act gct cca cag 211
Ala Ala Thr Ile Met Ala Thr Gly Ala Leu Ala Gly Thr Ala Pro Gln
25 30 35
gcg aca gca gca gta gat acc act gcg cca tac gtg tcc tat gtc gtt 259
Ala Thr Ala Ala Val Asp Thr Thr Ala Pro Tyr Val Ser Tyr Val Val
40 45 50
gac att cct ggc aag gtt ggt gaa cca atc aag cct cag tac ctc acc 307
Asp Ile Pro Gly Lys Val Gly Glu Pro Ile Lys Pro Gln Tyr Leu Thr
55 60 65
atc tcc gat cag tcc gcc tac acc gtg acc ttc aag tac atg cca tcc 355
Ile Ser Asp Gln Ser Ala Tyr Thr Val Thr Phe Lys Tyr Met Pro Ser
70 75 80 85
tgg ttg aag tac gac gca aac aag aag atg ctg tac ggt acc cca acc 403
Trp Leu Lys Tyr Asp Ala Asn Lys Lys Met Leu Tyr Gly Thr Pro Thr
90 95 100
gag gtt gat gtc tgg act cct gag gta cat gtt gtt gat gctc at ggc 451
Glu Val Asp Val Trp Thr Pro Glu Val His Val Val Asp Ala His Gly
105 110 115
aac aag act gtc cgt tac ttc acc gtt gta gca gtc cca gca aac act 499
Asn Lys Thr Val Arg Tyr Phe Thr Val Val Ala Val Pro Ala Asn Thr
120 125 130
gca cca acc acc acc acg cca tct act cca aag cca tct acc ccg cag 547
Ala Pro Thr Thr Thr Thr Pro Ser Thr Pro Lys Pro Ser Thr Pro Gln
135 140 145
gtg acc acc cca act act ccg aag gcg cct acc ttg cca aag tcc acc 595
Val Thr Thr Pro Thr Thr Pro Lys Ala Pro Thr Leu Pro Lys Ser Thr
150 155 160 165
ttc gac tgg acc ttg tgg ggc tcc atc ttt ggt ttc tagttctcac 641
Phe Asp Trp Thr Leu Trp Gly Ser Ile Phe Gly Phe
170 175
tagcacctca aaaagcacca 661
<210>216
<211>177
<212>PRT
<213>谷氨酸棒状杆菌
<400>216
Met Lys Ala Ser Gln Thr Leu Thr Arg Asn Thr Arg Ala Arg Lys Leu
1 5 10 15
Gly Met Gly Ile Leu Ala Ala Thr Ile Met Ala Thr Gly Ala Leu Ala
20 25 30
Gly Thr Ala Pro Gln Ala Thr Ala Ala Val Asp Thr Thr Ala Pro Tyr
35 40 45
Val Ser Tyr Val Val Asp Ile Pro Gly Lys Val Gly Glu Pro Ile Lys
50 55 60
Pro Gln Tyr Leu Thr Ile Ser Asp Gln Ser Ala Tyr Thr Val Thr Phe
65 70 75 80
Lys Tyr Met Pro Ser Trp Leu Lys Tyr Asp Ala Asn Lys Lys Met Leu
85 90 95
Tyr Gly Thr Pro Thr Glu Val Asp Val Trp Thr Pro Glu Val His Val
100 105 110
Val Asp Ala His Gly Asn Lys Thr Val Arg Tyr Phe Thr Val Val Ala
115 120 125
Val Pro Ala Asn Thr Ala Pro Thr Thr Thr Thr Pro Ser Thr Pro Lys
130 135 140
Pro Ser Thr Pro Gln Val Thr Thr Pro Thr Thr Pro Lys Ala Pro Thr
145 150 155 160
Leu Pro Lys Ser Thr Phe Asp Trp Thr Leu Trp Gly Ser Ile Phe Gly
165 170 175
Phe
<210>217
<211>976
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(946)
<223>RXA01910
<400>217
accgttttaa aaggcgcaat acaccacacc ccaacaaact ttttgattgt tggggtgttt 60
gtctcagatc atgaacagca caatgtagga gaagattgat atg gca ttt ccg ctt 115
Met Ala Phe Pro Leu
1 5
cta gcg gtt gct ggc aca gtt gcc ccc gtt gca gca gga tgg gca aaa 163
Leu Ala Val Ala Gly Thr Val Ala Pro Val Ala Ala Gly Trp Ala Lys
10 15 20
gat aag ttt ctc agc aac tct cag aat aat cag cag gcc cag aat cag 211
Asp Lys Phe Leu Ser Asn Ser Gln Asn Asn Gln Gln Ala Gln Asn Gln
25 30 35
cag atg agt ttt gga cag gtc aac aac agc gca cag aat tct gga tca 259
Gln Met Ser Phe Gly Gln Val Asn Asn Ser Ala Gln Asn Ser Gly Ser
40 45 50
gaa aac agc ggt ttc atg ggt caa tac ggc aat ctg ggt gca ggt ttg 307
Glu Asn Ser Gly Phe Met Gly Gln Tyr Gly Asn Leu Gly Ala Gly Leu
55 60 65
gca ggt gct gct aca ggc gca ggg ctt gcg tat agc gat ttt gaa gat 355
Ala Gly Ala Ala Thr Gly Ala Gly Leu Ala Tyr Ser Asp Phe Glu Asp
70 75 80 85
ggt caa agt ttg tcc tcg aag gcc cgc aac atg gtc ggc aaa ggg ctc 403
Gly Gln Ser Leu Ser Ser Lys Ala Arg Asn Met Val Gly Lys Gly Leu
90 95 100
gct ggt gcc gga gct ggt gtg ttc acc aag ctt gcc aat gat gca att 451
Ala Gly Ala Gly Ala Gly Val Phe Thr Lys Leu Ala Asn Asp Ala Ile
105 110 115
cag gcc gag ggt ggc tcg atg aag gct agt gct tat tct gcc att gca 499
Gln Ala Glu Gly Gly Ser Met Lys Ala Ser Ala Tyr Ser Ala Ile Ala
120 125 130
ggc ggc ttg gga tcg tat ctc aaa gac ggc ggt ccg ggt gtg att aaa 547
Gly Gly Leu Gly Ser Tyr Leu Lys Asp Gly Gly Pro Gly Val Ile Lys
135 140 145
tcc gcg atg gcc agc ggt gca gcc ggt ttt ggt gcg gat aaa gtg cac 595
Ser Ala Met Ala Ser Gly Ala Ala Gly Phe Gly Ala Asp Lys Val His
150 155 160 165
gat aaa tta gct gag tct ggt cat gag ggg ttg gct gat tcc ttg tcg 643
Asp Lys Leu Ala Glu Ser Gly His Glu Gly Leu Ala Asp Ser Leu Ser
170 175 180
ggc gct att caa gga ggt ggt ctt ggt tac tcc acg ctt ggc ggt gtc 691
Gly Ala Ile Gln Gly Gly Gly Leu Gly Tyr Ser Thr Leu Gly Gly Val
185 190 195
act ggt gct ggt att ggc ggt gcg acg ggc ggt ctc gca gga cta gca 739
Thr Gly Ala Gly Ile Gly Gly Ala Thr Gly Gly Leu Ala Gly Leu Ala
200 205 210
caa aac tac ttt ggt ggt ggc gat gac tac agc aac gct ggg gca tct 787
Gln Asn Tyr Phe Gly Gly Gly Asp Asp Tyr Ser Asn Ala Gly Ala Ser
215 220 225
gca tcg ggg ttt agt gcc aac cag gtt aat agt gag atc agc acc gag 835
Ala Ser Gly Phe Ser Ala Asn Gln Val Asn Ser Glu Ile Ser Thr Glu
230 235 240 245
att ccg cag ttt gcg aat ctt ggt caa cca cag cga tcc gag ctt gaa 883
Ile Pro Gln Phe Ala Asn Leu Gly Gln Pro Gln Arg Ser Glu Leu Glu
250 255 260
caa tta gcg cta cct caa gaa tca cgg tct gta gat aag agc tac gac 931
Gln Leu Ala Leu Pro Gln Glu Ser Arg Ser Val Asp Lys Ser Tyr Asp
265 270 275
caa ggc tac gaa gcg taagcgcttt ataacaaccc cgttttcagc 976
Gln Gly Tyr Glu Ala
280
<210>218
<211>282
<212>PRT
<213>谷氨酸棒状杆菌
<400>218
Met Ala Phe Pro Leu Leu Ala Val Ala Gly Thr Val Ala Pro Val Ala
1 5 10 15
Ala Gly Trp Ala Lys Asp Lys Phe Leu Ser Asn Ser Gln Asn Asn Gln
20 25 30
Gln Ala Gln Asn Gln Gln Met Ser Phe Gly Gln Val Asn Asn Ser Ala
35 40 45
Gln Asn Ser Gly Ser Glu Asn Ser Gly Phe Met Gly Gln Tyr Gly Asn
50 55 60
Leu Gly Ala Gly Leu Ala Gly Ala Ala Thr Gly Ala Gly Leu Ala Tyr
65 70 75 80
Ser Asp Phe Glu Asp Gly Gln Ser Leu Ser Ser Lys Ala Arg Asn Met
85 90 95
Val Gly Lys Gly Leu Ala Gly Ala Gly Ala Gly Val Phe Thr Lys Leu
100 105 110
Ala Asn Asp Ala Ile Gln Ala Glu Gly Gly Ser Met Lys Ala Ser Ala
115 120 125
Tyr Ser Ala Ile Ala Gly Gly Leu Gly Ser Tyr Leu Lys Asp Gly Gly
130 135 140
Pro Gly Val Ile Lys Ser Ala Met Ala Ser Gly Ala Ala Gly Phe Gly
145 150 155 160
Ala Asp Lys Val His Asp Lys Leu Ala Glu Ser Gly His Glu Gly Leu
165 170 175
Ala Asp Ser Leu Ser Gly Ala Ile Gln Gly Gly Gly Leu Gly Tyr Ser
180 185 190
Thr Leu Gly Gly Val Thr Gly Ala Gly Ile Gly Gly Ala Thr Gly Gly
195 200 205
Leu Ala Gly Leu Ala Gln Asn Tyr Phe Gly Gly Gly Asp Asp Tyr Ser
210 215 220
Asn Ala Gly Ala Ser Ala Ser Gly Phe Ser Ala Asn Gln Val Asn Ser
225 230 235 240
Glu Ile Ser Thr Glu Ile Pro Gln Phe Ala Asn Leu Gly Gln Pro Gln
245 250 255
Arg Ser Glu Leu Glu Gln Leu Ala Leu Pro Gln Glu Ser Arg Ser Val
260 265 270
Asp Lys Ser Tyr Asp Gln Gly Tyr Glu Ala
275 280
<210>219
<211>4432
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(4402)
<223>RXA01945
<400>219
attggatcta ggaaaaatta tgaacatctc aaagctcgga ctgaggatcg cggtcacagc 60
aacggcgacc acaacggcgc ttgccctcgg tggcacgcac atg gca gtt ggt caa 115
Met Ala Val Gly Gln
1 5
gaa act gat ctg ctc aac gct atg gaa aca ctc gct ggg gtt ccc tca 163
Glu Thr Asp Leu Leu Asn Ala Met Glu Thr Leu Ala Gly Val Pro Ser
10 15 20
gac ttg gtg gtc act gaa atc ctc ccc gat acc acc agc tat gac aat 211
Asp Leu Val Val Thr Glu Ile Leu Pro Asp Thr Thr Ser Tyr Asp Asn
25 30 35
ttt gaa ttc ttt gaa gtt cac aac acc ggc agt gca ccc gtg acc att 259
Phe Glu Phe Phe Glu Val His Asn Thr Gly Ser Ala Pro Val Thr Ile
40 45 50
ggg gaa ggg gag tac acc ttc gcc tat tcc ttt gac gat tcc gcc gat 307
Gly Glu Gly Glu Tyr Thr Phe Ala Tyr Ser Phe Asp Asp Ser Ala Asp
55 60 65
acg tcc cgc gac aag gca ctg gat ctt ggc ggg gaa gtc acg gta gat 355
Thr Ser Arg Asp Lys Ala Leu Asp Leu Gly Gly Glu Val Thr Val Asp
70 75 80 85
gca ggc gaa acc att gtg gtg tgg att gag tac tcc agt tca act gtt 403
Ala Gly Glu Thr Ile Val Val Trp Ile Glu Tyr Ser Ser Ser Thr Val
90 95 100
gat acc gct gcg ttt agt gag caa gac ttc cgt gat ttc tac ggc atg 451
Asp Thr Ala Ala Phe Ser Glu Gln Asp Phe Arg Asp Phe Tyr Gly Met
105 110 115
gat tcc tca gcc cgc atc ttc cga gca act ggc cag gcg ggt ctc gct 499
Asp Ser Ser Ala Arg Ile Phe Arg Ala Thr Gly Gln Ala Gly Leu Ala
120 125 130
aac ggt ggt gat cgt ggc atc cga gtt ctg tac aat ggt gaa gtt tct 547
Asn Gly Gly Asp Arg Gly Ile Arg Val Leu Tyr Asn Gly Glu Val Ser
135 140 145
ggt tgg tcc cac tac cca tca gat agc gca gcg gtt caa aag gga att 595
Gly Trp Ser His Tyr Pro Ser Asp Ser Ala Ala Val Gln Lys Gly Ile
150 155 160 165
gac ttc gcg ctg cca aaa gta ggg gag cag gcc agc att gca gtg cgc 643
Asp Phe Ala Leu Pro Lys Val Gly Glu Gln Ala Ser Ile Ala Val Arg
170 175 180
acc aaa act gat cca act cca gga tca att act tct gat cag ttg att 691
Thr Lys Thr Asp Pro Thr Pro Gly Ser Ile Thr Ser Asp Gln Leu Ile
185 190 195
cct ggc gga ttg gac act cca gaa gag cct gaa gcc cct gaa gac tct 739
Pro Gly Gly Leu Asp Thr Pro Glu Glu Pro Glu Ala Pro Glu Asp Ser
200 205 210
ctt ttt gaa ggg cgc act ccc cct agg gat gca gca ccg ctg atc ctc 787
Leu Phe Glu Gly Arg Thr Pro Pro Arg Asp Ala Ala Pro Leu Ile Leu
215 220 225
act gaa cta atg gtc aac tcc acc aac atg ggc agt tct gat ggt ttt 835
Thr Glu Leu Met Val Asn Ser Thr Asn Met Gly Ser Ser Asp Gly Phe
230 235 240 245
gag tat gtg gaa atc acc aac act act gcc gaa ccc atc gat ttt tcc 883
Glu Tyr Val Glu Ile Thr Asn Thr Thr Ala Glu Pro Ile Asp Phe Ser
250 255 260
gat tac acc ttg aac tac ctg tac ccg cag gat gag ttc acc aac acc 931
Asp Tyr Thr Leu Asn Tyr Leu Tyr Pro Gln Asp Glu Phe Thr Asn Thr
265 270 275
aac gaa gcc gtc tgg gct gcg gaa cct ggt gat gtc att att cag cct 979
Asn Glu Ala Val Trp Ala Ala Glu Pro Gly Asp Val Ile Ile Gln Pro
280 285 290
gga aaa tct ctc gtg ttt tgg atc aaa aat ggc ccc aat gat gag gca 1027
Gly Lys Ser Leu Val Phe Trp Ile Lys Asn Gly Pro Asn Asp Glu Ala
295 300 305
acc gca gca gat ttc aat gca gaa tat ggc acc aac ctg gag gct gga 1075
Thr Ala Ala Asp Phe Asn Ala Glu Tyr Gly Thr Asn Leu Glu Ala Gly
310 315 320 325
aaa gac ctt gtt gaa atc tcc tca ggt ggg atg gcc aat ggt act gcc 1123
Lys Asp Leu Val Glu Ile Ser Ser Gly Gly Met Ala Asn Gly Thr Ala
330 335 340
aga gga atg cag att cag acc aac act ggc cac ata gtc aac cgt ggt 1171
Arg Gly Met Gln Ile Gln Thr Asn Thr Gly His Ile Val Asn Arg Gly
345 350 355
ttc tac aac atg gct ggc gct tct gat gtg aaa gcc aac gag ggt ctt 1219
Phe Tyr Asn Met Ala Gly Ala Ser Asp Val Lys Ala Asn Glu Gly Leu
360 365 370
cat ttc gca gtg gat gag tct gat ctt ctg aag caa acg ctt gtc ggc 1267
His Phe Ala Val Asp Glu Ser Asp Leu Leu Lys Gln Thr Leu Val Gly
375 380 385
agc ggt gcg cca acc ccg ggc act gtg tac act tcg cag att cct aat 1315
Ser Gly Ala Pro Thr Pro Gly Thr Val Tyr Thr Ser Gln Ile Pro Asn
390 395 400 405
cca ctg tct gcc gtt atc gct gat tct tct gta cca ctc atc aca gat 1363
Pro Leu Ser Ala Val Ile Ala Asp Ser Ser Val Pro Leu Ile Thr Asp
410 415 420
aat acc gca acc agt atc aac cct gcg gag ccg ttt acc ttc gcc ttc 1411
Asn Thr Ala Thr Ser Ile Asn Pro Ala Glu Pro Phe Thr Phe Ala Phe
425 430 435
aat atc acc gat gat gtc cag gtg cgc acg gca aca ctg cat gtt act 1459
Asn Ile Thr Asp Asp Val Gln Val Arg Thr Ala Thr Leu His Val Thr
440 445 450
tcc agt gct ggc gaa gcc gcg aca acc atc aac ctc acc gag gat gac 1507
Ser Ser Ala Gly Glu Ala Ala Thr Thr Ile Asn Leu Thr Glu Asp Asp
455 460 465
ggc tct ttc aat tgg gct ctg cct gca gcg gat ctc acc gga aaa tcc 1555
Gly Ser Phe Asn Trp Ala Leu Pro Ala Ala Asp Leu Thr Gly Lys Ser
470 475 480 485
tgg ttc gaa tac acc gta acc gcc acc gac gga ttc aac agc gtt acc 1603
Trp Phe Glu Tyr Thr Val Thr Ala Thr Asp Gly Phe Asn Ser Val Thr
490 495 500
acc gag ccg gta cgc gtc acc gtc gac ggc gcc aac acc gac ccg ctg 1651
Thr Glu Pro Val Arg Val Thr Val Asp Gly Ala Asn Thr Asp Pro Leu
505 510 515
cgc ctc aac ctg gaa gaa aac caa tgg gtc agt ggc acc acc gat gtt 1699
Arg Leu Asn Leu Glu Glu Asn Gln Trp Val Ser Gly Thr Thr Asp Val
520 525 530
atc ggt gct tca gat gtc ttc ggc gac aag ctt gaa ttg ctt atc gac 1747
Ile Gly Ala Ser Asp Val Phe Gly Asp Lys Leu Glu Leu Leu Ile Asp
535 540 545
gac gcg cct gca gtc acc aac tcc agc ctg tct gcg gcc ccg acg ttt 1795
Asp Ala Pro Ala Val Thr Asn Ser Ser Leu Ser Ala Ala Pro Thr Phe
550 555 560 565
gcg atg gaa gta acc caa act gat gtg ttc ttc cgc aac ggc atc ctt 1843
Ala Met Glu Val Thr Gln Thr Asp Val Phe Phe Arg Asn Gly Ile Leu
570 575 580
gcc ggt ggg gaa gaa ctc cgc att ttc gat caa gga act tac gcc aac 1891
Ala Gly Gly Glu Glu Leu Arg Ile Phe Asp Gln Gly Thr Tyr Ala Asn
585 590 595
acc gaa acc atc tcc aca cca gtc ccg ctg tat cac atc aat gag gac 1939
Thr Glu Thr Ile Ser Thr Pro Val Pro Leu Tyr His Ile Asn Glu Asp
600 605 610
ggt acc ctc aca gtc agt gtg tat gcg gga act aaa gca gca cca gaa 1987
Gly Thr Leu Thr Val Ser Val Tyr Ala Gly Thr Lys Ala Ala Pro Glu
615 620 625
att gac ctc aac gag aac aat gac gat ttc cag atc aga aac ctt cga 2035
Ile Asp Leu Asn Glu Asn Asn Asp Asp Phe Gln Ile Arg Asn Leu Arg
630 635 640 645
cta att ctg cct gat ggc cgg acc ctc acc cct gcc gga att tcc gat 2083
Leu Ile Leu Pro Asp Gly Arg Thr Leu Thr Pro Ala Gly Ile Ser Asp
650 655 660
tct aat gcg tgg ctc aac atg gga gac agc gct gga aaa ctc gat ttc 2131
Ser Asn Ala Trp Leu Asn Met Gly Asp Ser Ala Gly Lys Leu Asp Phe
665 670 675
ttc gat gcc acc ttc gcc ctc cct gag gat gct ttc acc ggt gtg gca 2179
Phe Asp Ala Thr Phe Ala Leu Pro Glu Asp Ala Phe Thr Gly Val Ala
680 685 690
cac gca tgg gat acc acc caa agc aca gat gga gaa cac cac atc acc 2227
His Ala Trp Asp Thr Thr Gln Ser Thr Asp Gly Glu His His Ile Thr
695 700 705
att tcc cgc gaa gac ggc ggg gaa atc agc cgc acc atc cgg gtt gat 2275
Ile Ser Arg Glu Asp Gly Gly Glu Ile Ser Arg Thr Ile Arg Val Asp
710 715 720 725
aat act gcc cca gaa ctc acc gtt tct gga gtt gaa gaa gga caa gaa 2323
Asn Thr Ala Pro Glu Leu Thr Val Ser Gly Val Glu Glu Gly Gln Glu
730 735 740
ctg cgc ggc acc gta gaa att gat gcc cag gca acc gat gcg ggt gcg 2371
Leu Arg Gly Thr Val Glu Ile Asp Ala Gln Ala Thr Asp Ala Gly Ala
745 750 755
ggc gtg aag agc gtc gag acg ctt ctc gac ggc gaa cgc gtg caa ctt 2419
Gly Val Lys Ser Val Glu Thr Leu Leu Asp Gly Glu Arg Val Gln Leu
760 765 770
cca cta acc acc ggt tcc atc gct ttg gat aaa ggt gaa cac acc ttg 2467
Pro Leu Thr Thr Gly Ser Ile Ala Leu Asp Lys Gly Glu His Thr Leu
775 780 785
gtt atc cgt gca gaa gat gaa gta gga aac cgc acc gag aaa acc atc 2515
Val Ile Arg Ala Glu Asp Glu Val Gly Asn Arg Thr Glu Lys Thr Ile
790 795 800 805
acg ttt agc act ccg gat gaa aac ccc atc agt ggt gac tac gct cca 2563
Thr Phe Ser Thr Pro Asp Glu Asn Pro Ile Ser Gly Asp Tyr Ala Pro
810 815 820
agc aat ggg gcc acc gtg ggc gtc ggt gac gtt aag tta tct gca cga 2611
Ser Asn Gly Ala Thr Val Gly Val Gly Asp Val Lys Leu Ser Ala Arg
825 830 835
gca agt gat cca agt ggc gat act gtc aag atg acg ttc ctg gaa gcc 2659
Ala Ser Asp Pro Ser Gly Asp Thr Val Lys Met Thr Phe Leu Glu Ala
840 845 850
gat tca cca aaa tta gat agt ggt cgc gtc cga atg tca tca gga acg 2707
Asp Ser Pro Lys Leu Asp Ser Gly Arg Val Arg Met Ser Ser Gly Thr
855 860 865
gta gaa gat gcc gga agt gtc tcg cgc gcc gag gcg aaa atg ttg gag 2755
Val Glu Asp Ala Gly Ser Val Ser Arg Ala Glu Ala Lys Met Leu Glu
870 875 880 885
agg gga gac gtc gag aag cta tcc agc ctg gat ggg ctg ggc atg gaa 2803
Arg Gly Asp Val Glu Lys Leu Ser Ser Leu Asp Gly Leu Gly Met Glu
890 895 900
gtt acc tcc gac gcc gca ctg ccg tac cag ctt ttt gaa gtc gat gcg 2851
Val Thr Ser Asp Ala Ala Leu Pro Tyr Gln Leu Phe Glu Val Asp Ala
905 910 915
gcg gat gca ctc gcg gcc gac act gaa gtg cgc ctg aat tgg gcg gga 2899
Ala Asp Ala Leu Ala Ala Asp Thr Glu Val Arg Leu Asn Trp Ala Gly
920 925 930
tcc gcc gat ggt cgc gcg cag gtg atc atg tat gtt ttc gat ggc gag 2947
Ser Ala Asp Gly Arg Ala Gln Val Ile Met Tyr Val Phe Asp Gly Glu
935 940 945
gcg tgg gtt gag gtg gat cgt cac ttg acc ggc gat gag ctg gaa gag 2995
Ala Trp Val Glu Val Asp Arg His Leu Thr Gly Asp Glu Leu Glu Glu
950 955 960 965
ttt acg ctg cag ggc gtc gtc aat gcg gaa aaa ttt gca atc ggc ggc 3043
Phe Thr Leu Gln Gly Val Val Asn Ala Glu Lys Phe Ala Ile Gly Gly
970 975 980
act gtc acc gta ttg att cag cac tcc gaa ggc ttc gcc ggt gcg gat 3091
Thr Val Thr Val Leu Ile Gln His Ser Glu Gly Phe Ala Gly Ala Asp
985 990 995
cat tca act aga aat tcc gac gtg acc gca gcg cac ccg gat gat gtg 3139
His Ser Thr Arg Asn Ser Asp Val Thr Ala Ala His Pro Asp Asp Val
1000 1005 1010
gct cgc tct gag tac gat ttc acc ctc gcg tgg gaa tct gac acc cag 3187
Ala Arg Ser Glu Tyr Asp Phe Thr Leu Ala Trp Glu Ser Asp Thr Gln
1015 1020 1025
tac tac aac gag gaa ttc cac gag cac caa acc aac atc cat gac tac 3235
Tyr Tyr Asn Glu Glu Phe His Glu His Gln Thr Asn Ile His Asp Tyr
1030 1035 1040 1045
gtg ctc gcc gaa cgg gag aac aag aat att cag ttc atg ttc cac act 3283
Val Leu Ala Glu Arg Glu Asn Lys Asn Ile Gln Phe Met Phe His Thr
1050 1055 1060
ggc gat gtt gtc gac gac tgg gat cag ccc gcg cag tgg gcc aca gcc 3331
Gly Asp Val Val Asp Asp Trp Asp Gln Pro Ala Gln Trp Ala Thr Ala
1065 1070 1075
aac ccc gaa tac cag cgc ctc gac gac gcc ggc ctg cca tat tct gtc 3379
Asn Pro Glu Tyr Gln Arg Leu Asp Asp Ala Gly Leu Pro Tyr Ser Val
1080 1085 1090
ctt gcc gga aac cac gat gtt ggc cac acc agc aat gac tac acc gaa 3427
Leu Ala Gly Asn His Asp Val Gly His Thr Ser Asn Asp Tyr Thr Glu
1095 1100 1105
ttc agc cga cac ttc ggc gaa cag cgc tac gta gac aac ccg tgg tac 3475
Phe Ser Arg His Phe Gly Glu Gln Arg Tyr Val Asp Asn Pro Trp Tyr
1110 1115 1120 1125
ggc gaa tcc tac caa gac aac cga ggg cac tac gat cta ttt tct gcc 3523
Gly Glu Ser Tyr Gln Asp Asn Arg Gly His Tyr Asp Leu Phe Ser Ala
1130 1135 1140
ggc gga att gac ttc att aac gta gcg atg ggc tgg ggt cca gac gac 3571
Gly Gly Ile Asp Phe Ile Asn Val Ala Met Gly Trp Gly Pro Asp Asp
1145 1150 1155
gaa gaa atc gcg tgg atg aac gag gtc ctg gcc aag cat ccc gag cgt 3619
Glu Glu Ile Ala Trp Met Asn Glu Val Leu Ala Lys His Pro Glu Arg
1160 1165 1170
gtg gcg atc ctc aac ctc cac gaa ttc atg ctc acc acc ggc gga ctt 3667
Val Ala Ile Leu Asn Leu His Glu Phe Met Leu Thr Thr Gly Gly Leu
1175 1180 1185
ggc ccg atc ccg cag cgc att ctc gac gag gtc gca gcc acc aac cca 3715
Gly Pro Ile Pro Gln Arg Ile Leu Asp Glu Val Ala Ala Thr Asn Pro
1190 1195 1200 1205
aat gtc agc atg atc atg tcc ggc cac tac cac gac gca ttc caa cgc 3763
Asn Val Ser Met Ile Met Ser Gly His Tyr His Asp Ala Phe Gln Arg
1210 1215 1220
acc gac tcc ttt gac gac gat ggt gat gga gta gat gac cgc acc gtc 3811
Thr Asp Ser Phe Asp Asp Asp Gly Asp Gly Val Asp Asp Arg Thr Val
1225 1230 1235
acc tct atg ctt ttc gat tac caa ggc cta ccg gag ggc gga cag ggg 3859
Thr Ser Met Leu Phe Asp Tyr Gln Gly Leu Pro Glu Gly Gly Gln Gly
1240 1245 1250
tac ctc cga ctt ctc cac ttt gat aac caa ggc caa aag atg atg gtg 3907
Tyr Leu Arg Leu Leu His Phe Asp Asn Gln Gly Gln Lys Met Met Val
1255 1260 1265
cgc acc tat tca cca tcc ctg aag gat tac aac tct gat gaa ccc tca 3955
Arg Thr Tyr Ser Pro Ser Leu Lys Asp Tyr Asn Ser Asp Glu Pro Ser
1270 1275 1280 1285
ctg ttg ggg cct gca gaa gac ccc aac atg tat caa gaa ttc gaa gtg 4003
Leu Leu Gly Pro Ala Glu Asp Pro Asn Met Tyr Gln Glu Phe Glu Val
1290 1295 1300
tcc tac gag cag ctc ggc atc aaa cca gag ggc cgc acc ctg atc ggc 4051
Ser Tyr Glu Gln Leu Gly Ile Lys Pro Glu Gly Arg Thr Leu Ile Gly
1305 1310 1315
gat tcc ttc agc gcc gat ttc ttg acc tcc aat gaa att gga ata gtt 4099
Asp Ser Phe Ser Ala Asp Phe Leu Thr Ser Asn Glu Ile Gly Ile Val
1320 1325 1330
gat gag gtt cct tct gga acg atc gct ttc acg aac tgg aag gac gta 4147
Asp Glu Val Pro Ser Gly Thr Ile Ala Phe Thr Asn Trp Lys Asp Val
1335 1340 1345
acc gaa ggt cgc cac agt tgg tat gtt cgc tcc gag gat cct ttc ggc 4195
Thr Glu Gly Arg His Ser Trp Tyr Val Arg Ser Glu Asp Pro Phe Gly
1350 1355 1360 1365
ggc gtc gag att tca ccc gtg cag tcc ttc att gcc ggg gaa gag gct 4243
Gly Val Glu Ile Ser Pro Val Gln Ser Phe Ile Ala Gly Glu Glu Ala
1370 1375 1380
ggc ggg aac gcg ccc ggc act gga agc tcc aat ggc ggt tca tcc cac 4291
Gly Gly Asn Ala Pro Gly Thr Gly Ser Ser Asn Gly Gly Ser Ser His
1385 1390 1395
gga tta tgg ggt gcg ctt gcg gga ttc ttt gcc gga gcg gca gcc ctg 4339
Gly Leu Trp Gly Ala Leu Ala Gly Phe Phe Ala Gly Ala Ala Ala Leu
1400 1405 1410
gct gga gct gcg atc gca ttt gtc ccc gga att tgg gac tat gtg acc 4387
Ala Gly Ala Ala Ile Ala Phe Val Pro Gly Ile Trp Asp Tyr Val Thr
1415 1420 1425
aac gca ttc aag cga taattatgga taggtaaacg ctctacacgg 4432
Asn Ala Phe Lys Arg
1430
<210>220
<211>1434
<212>PRT
<213>谷氨酸棒状杆菌
<400>220
Met Ala Val Gly Gln Glu Thr Asp Leu Leu Asn Ala Met Glu Thr Leu
1 5 10 15
Ala Gly Val Pro Ser Asp Leu Val Val Thr Glu Ile Leu Pro Asp Thr
20 25 30
Thr Ser Tyr Asp Asn Phe Glu Phe Phe Glu Val His Asn Thr Gly Ser
35 40 45
Ala Pro Val Thr Ile Gly Glu Gly Glu Tyr Thr Phe Ala Tyr Ser Phe
50 55 60
Asp Asp Ser Ala Asp Thr Ser Arg Asp Lys Ala Leu Asp Leu Gly Gly
65 70 75 80
Glu Val Thr Val Asp Ala Gly Glu Thr Ile Val Val Trp Ile Glu Tyr
85 90 95
SerSer Ser Thr Val Asp Thr Ala Ala Phe Ser Glu Gln Asp Phe Arg
100 105 110
Asp Phe Tyr Gly Met Asp Ser Ser Ala Arg Ile Phe Arg Ala Thr Gly
115 120 125
Gln Ala Gly Leu Ala Asn Gly Gly Asp Arg Gly Ile Arg Val Leu Tyr
130 135 140
Asn Gly Glu Val Ser Gly Trp Ser His Tyr Pro Ser Asp Ser Ala Ala
145 150 155 160
Val Gln Lys Gly Ile Asp Phe Ala Leu Pro Lys Val Gly Glu Gln Ala
165 170 175
Ser Ile Ala Val Arg Thr Lys Thr Asp Pro Thr Pro Gly Ser Ile Thr
180 185 190
Ser Asp Gln Leu Ile Pro Gly Gly Leu Asp Thr Pro Glu Glu Pro Glu
195 200 205
Ala Pro Glu Asp Ser Leu Phe Glu Gly Arg Thr Pro Pro Arg Asp Ala
210 215 220
Ala Pro Leu Ile Leu Thr Glu Leu Met Val Asn Ser Thr Asn Met Gly
225 230 235 240
Ser Ser Asp Gly Phe Glu Tyr Val Glu Ile Thr Asn Thr Thr Ala Glu
245 250 255
Pro Ile Asp Phe Ser Asp Tyr Thr Leu Asn Tyr Leu Tyr Pro Gln Asp
260 265 270
Glu Phe Thr Asn Thr Asn Glu Ala Val Trp Ala Ala Glu Pro Gly Asp
275 280 285
Val Ile Ile Gln Pro Gly Lys Ser Leu Val Phe Trp Ile Lys Asn Gly
290 295 300
Pro Asn Asp Glu Ala Thr Ala Ala Asp Phe Asn Ala Glu Tyr Gly Thr
305 310 315 320
Asn Leu Glu Ala Gly Lys Asp Leu Val Glu Ile Ser Ser Gly Gly Met
325 330 335
Ala Asn Gly Thr Ala Arg Gly Met Gln Ile Gln Thr Asn Thr Gly His
340 345 350
Ile Val Asn Arg Gly Phe Tyr Asn Met Ala Gly Ala Ser Asp Val Lys
355 360 365
Ala Asn Glu Gly Leu His Phe Ala Val Asp Glu Ser Asp Leu Leu Lys
370 375 380
Gln Thr Leu Val Gly Ser Gly Ala Pro Thr Pro Gly Thr Val Tyr Thr
385 390 395 400
Ser Gln Ile Pro Asn Pro Leu Ser Ala Val Ile Ala Asp Ser Ser Val
405 410 415
Pro Leu Ile Thr Asp Asn Thr Ala Thr Ser Ile Asn Pro Ala Glu Pro
420 425 430
Phe Thr Phe Ala Phe Asn Ile Thr Asp Asp Val Gln Val Arg Thr Ala
435 440 445
Thr Leu His Val Thr Ser Ser Ala Gly Glu Ala Ala Thr Thr Ile Asn
450 455 460
Leu Thr Glu Asp Asp Gly Ser Phe Asn Trp Ala Leu Pro Ala Ala Asp
465 470 475 480
Leu Thr Gly Lys Ser Trp Phe Glu Tyr Thr Val Thr Ala Thr Asp Gly
485 490 495
Phe Asn Ser Val Thr Thr Glu Pro Val Arg Val Thr Val Asp Gly Ala
500 505 510
Asn Thr Asp Pro Leu Arg Leu Asn Leu Glu Glu Asn Gln Trp Val Ser
515 520 525
Gly Thr Thr Asp Val Ile Gly Ala Ser Asp Val Phe Gly Asp Lys Leu
530 535 540
Glu Leu Leu Ile Asp Asp Ala Pro Ala Val Thr Asn Ser Ser Leu Ser
545 550 555 560
Ala Ala Pro Thr Phe Ala Met Glu Val Thr Gln Thr Asp Val Phe Phe
565 570 575
Arg Asn Gly Ile Leu Ala Gly Gly Glu Glu Leu Arg Ile Phe Asp Gln
580 585 590
Gly Thr Tyr Ala Asn Thr Glu Thr Ile Ser Thr Pro Val Pro Leu Tyr
595 600 605
His Ile Asn Glu Asp Gly Thr Leu Thr Val Ser Val Tyr Ala Gly Thr
610 615 620
Lys Ala Ala Pro Glu Ile Asp Leu Asn Glu Asn Asn Asp Asp Phe Gln
625 630 635 640
Ile Arg Asn Leu Arg Leu Ile Leu Pro Asp Gly Arg Thr Leu Thr Pro
645 650 655
Ala Gly Ile Ser Asp Ser Asn Ala Trp Leu Asn Met Gly Asp Ser Ala
660 665 670
Gly Lys Leu Asp Phe Phe Asp Ala Thr Phe Ala Leu Pro Glu Asp Ala
675 680 685
Phe Thr Gly Val Ala His Ala Trp Asp Thr Thr Gln Ser Thr Asp Gly
690 695 700
Glu His His Ile Thr Ile Ser Arg Glu Asp Gly Gly Glu Ile Ser Arg
705 710 715 720
Thr Ile Arg Val Asp Asn Thr Ala Pro Glu Leu Thr Val Ser Gly Val
725 730 735
Glu Glu Gly Gln Glu Leu Arg Gly Thr Val Glu Ile Asp Ala Gln Ala
740 745 750
Thr Asp Ala Gly Ala Gly Val Lys Ser Val Glu Thr Leu Leu Asp Gly
755 760 765
Glu Arg Val Gln Leu Pro Leu Thr Thr Gly Ser Ile Ala Leu Asp Lys
770 775 780
Gly Glu His Thr Leu Val Ile Arg Ala Glu Asp Glu Val Gly Asn Arg
785 790 795 800
Thr Glu Lys Thr Ile Thr Phe Ser Thr Pro Asp Glu Asn Pro Ile Ser
805 810 815
Gly Asp Tyr Ala Pro Ser Asn Gly Ala Thr Val Gly Val Gly Asp Val
820 825 830
Lys Leu Ser Ala Arg Ala Ser Asp Pro Ser Gly Asp Thr Val Lys Met
835 840 845
Thr Phe Leu Glu Ala Asp Ser Pro Lys Leu Asp Ser Gly Arg Val Arg
850 855 860
Met Ser Ser Gly Thr Val Glu Asp Ala Gly Ser Val Ser Arg Ala Glu
865 870 875 880
Ala Lys Met Leu Glu Arg Gly Asp Val Glu Lys Leu Ser Ser Leu Asp
885 890 895
Gly Leu Gly Met Glu Val Thr Ser Asp Ala Ala Leu Pro Tyr Gln Leu
900 905 910
Phe Glu Val Asp Ala Ala Asp Ala Leu Ala Ala Asp Thr Glu Val Arg
915 920 925
Leu Asn Trp Ala Gly Ser Ala Asp Gly Arg Ala Gln Val Ile Met Tyr
930 935 940
Val Phe Asp Gly Glu Ala Trp Val Glu Val Asp Arg His Leu Thr Gly
945 950 955 960
Asp Glu Leu Glu Glu Phe Thr Leu Gln Gly Val Val Asn Ala Glu Lys
965 970 975
Phe Ala Ile Gly Gly Thr Val Thr Val Leu Ile Gln His Ser Glu Gly
980 985 990
Phe Ala Gly Ala Asp His Ser Thr Arg Asn Ser Asp Val Thr Ala Ala
995 1000 1005
His Pro Asp Asp Val Ala Arg Ser Glu Tyr Asp Phe Thr Leu Ala Trp
1010 1015 1020
Glu Ser Asp Thr Gln Tyr Tyr Asn Glu Glu Phe His Glu His Gln Thr
1025 1030 1035 1040
Asn Ile His Asp Tyr Val Leu Ala Glu Arg Glu Asn Lys Asn Ile Gln
1045 1050 1055
Phe Met Phe His Thr Gly Asp Val Val Asp Asp Trp Asp Gln Pro Ala
1060 1065 1070
Gln Trp Ala Thr Ala Asn Pro Glu Tyr Gln Arg Leu Asp Asp Ala Gly
1075 1080 1085
Leu Pro Tyr Ser Val Leu Ala Gly Asn His Asp Val Gly His Thr Ser
1090 1095 1100
Asn Asp Tyr Thr Glu Phe Ser Arg His Phe Gly Glu Gln Arg Tyr Val
1105 1110 1115 1120
Asp Asn Pro Trp Tyr Gly Glu Ser Tyr Gln Asp Asn Arg Gly His Tyr
1125 1130 1135
Asp Leu Phe Ser Ala Gly Gly Ile Asp Phe Ile Asn Val Ala Met Gly
1140 1145 1150
Trp Gly Pro Asp Asp Glu Glu Ile Ala Trp Met Asn Glu Val Leu Ala
1155 1160 1165
Lys His Pro Glu Arg Val Ala Ile Leu Asn Leu His Glu Phe Met Leu
1170 1175 1180
Thr Thr Gly Gly Leu Gly Pro Ile Pro Gln Arg Ile Leu Asp Glu Val
1185 1190 1195 1200
Ala Ala Thr Asn Pro Asn Val Ser Met Ile Met Ser Gly His Tyr His
1205 1210 1215
Asp Ala Phe Gln Arg Thr Asp Ser Phe Asp Asp Asp Gly Asp Gly Val
1220 1225 1230
Asp Asp Arg Thr Val Thr Ser Met Leu Phe Asp Tyr Gln Gly Leu Pro
1235 1240 1245
Glu Gly Gly Gln Gly Tyr Leu Arg Leu Leu His Phe Asp Asn Gln Gly
1250 1255 1260
Gln Lys Met Met Val Arg Thr Tyr Ser Pro Ser Leu Lys Asp Tyr Asn
1265 1270 1275 1280
Ser Asp Glu Pro Ser Leu Leu Gly Pro Ala Glu Asp Pro Asn Met Tyr
1285 1290 1295
Gln Glu Phe Glu Val Ser Tyr Glu Gln Leu Gly Ile Lys Pro Glu Gly
1300 1305 1310
Arg Thr Leu Ile Gly Asp Ser Phe Ser Ala Asp Phe Leu Thr Ser Asn
1315 1320 1325
Glu Ile Gly Ile Val Asp Glu Val Pro Ser Gly Thr Ile Ala Phe Thr
1330 1335 1340
Asn Trp Lys Asp Val Thr Glu Gly Arg His Ser Trp Tyr Val Arg Ser
1345 1350 1355 1360
Glu Asp Pro Phe Gly Gly Val Glu Ile Ser Pro Val Gln Ser Phe Ile
1365 1370 1375
Ala Gly Glu Glu Ala Gly Gly Asn Ala Pro Gly Thr Gly Ser Ser Asn
1380 1385 1390
Gly Gly Ser Ser His Gly Leu Trp Gly Ala Leu Ala Gly Phe Phe Ala
1395 1400 1405
Gly Ala Ala Ala Leu Ala Gly Ala Ala Ile Ala Phe Val Pro Gly Ile
1410 1415 1420
Trp Asp Tyr Val Thr Asn Ala Phe Lys Arg
1425 1430
<210>221
<211>787
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(757)
<223>RXA01966
<400>221
gctgtccaag gccatgaatt ttgtgtcctc tgttgctcca accgctgtgc tctcccctgt 60
tatgggctgg gtttataaga agatgggtta gtttttaaaa gtg tct gaa tct gaa 115
Val Ser Glu Ser Glu
1 5
aac aac aca aca cca gca gtc gca gct cgc gat gac cgt ctg gtc tgg 163
Asn Asn Thr Thr Pro Ala Val Ala Ala Arg Asp Asp Arg Leu Val Trp
10 15 20
gtc gat ctg gaa atg act ggt cta gat ttg aag cgc cac gtg atc gtg 211
Val Asp Leu Glu Met Thr Gly Leu Asp Leu Lys Arg His Val Ile Val
25 30 35
gag gtt gcg gcg ttg gtc act gac gct aac ctc aac gtt ttg ggc gag 259
Glu Val Ala Ala Leu Val Thr Asp Ala Asn Leu Asn Val Leu Gly Glu
40 45 50
ggc gtg gac ttg gtt gtt cac gca act gaa gaa gag ctc gcg cag atg 307
Gly Val Asp Leu Val Val His Ala Thr Glu Glu Glu Leu Ala Gln Met
55 60 65
gat gat ttt gtc acc aac atg cac gaa tcc tct ggg ctg act gag cag 355
Asp Asp Phe Val Thr Asn Met His Glu Ser Ser Gly Leu Thr Glu Gln
70 75 80 85
atc cgg gaa tcc gcg gtc acg ttg aag gaa gcc gaa gat gct gtg ctc 403
Ile Arg Glu Ser Ala Val Thr Leu Lys Glu Ala Glu Asp Ala Val Leu
90 95 100
gca ttg att gaa aag cac tgc gat cca gcc cat cct gca ccg cta gct 451
Ala Leu Ile Glu Lys His Cys Asp Pro Ala His Pro Ala Pro Leu Ala
105 110 115
ggt aac tcc att gcc act gac cgc gcg ttt atc cgc gaa cat atg cca 499
Gly Asn Ser Ile Ala Thr Asp Arg Ala Phe Ile Arg Glu His Met Pro
120 125 130
cgt ctt gat gag gcc ctg cat tac cgc atg gtg gat gtg tcc tcg gtg 547
Arg Leu Asp Glu Ala Leu His Tyr Arg Met Val Asp Val Ser Ser Val
135 140 145
aag gaa ttg gcg cgt cgc tgg tac cca cgc gtg tac tac aag cag ccg 595
Lys Glu Leu Ala Arg Arg Trp Tyr Pro Arg Val Tyr Tyr Lys Gln Pro
150 155 160 165
gag aag ggt ttg gcg cac cgc gcg ttg gcg gac att gtg gag tcg att 643
Glu Lys Gly Leu Ala His Arg Ala Leu Ala Asp Ile Val Glu Ser Ile
170 175 180
cgg gag ttg gat tac tac cgt cgc tca ttt ttt gtt gca gag cct ggt 691
Arg Glu Leu Asp Tyr Tyr Arg Arg Ser Phe Phe Val Ala Glu Pro Gly
185 190 195
cct acc tct gag cag tgc gca gat gat gcg cag gca gcg gtg gac cgt 739
Pro Thr Ser Glu Gln Cys Ala Asp Asp Ala Gln Ala Ala Val Asp Arg
200 205 210
ttt gca ccc tac ttt gat tagagggttt taagcagcct ggtgagttcg 787
Phe Ala Pro Tyr Phe Asp
215
<210>222
<211>219
<212>PRT
<213>谷氨酸棒状杆菌
<400>222
Val Ser Glu Ser Glu Asn Asn Thr Thr Pro Ala Val Ala Ala Arg Asp
1 5 10 15
Asp Arg Leu Val Trp Val Asp Leu Glu Met Thr Gly Leu Asp Leu Lys
20 25 30
Arg His Val Ile Val Glu Val Ala Ala Leu Val Thr Asp Ala Asn Leu
35 40 45
Asn Val Leu Gly Glu Gly Val Asp Leu Val Val His Ala Thr Glu Glu
50 55 60
Glu Leu Ala Gln Met Asp Asp Phe Val Thr Asn Met His Glu Ser Ser
65 70 75 80
Gly Leu Thr Glu Gln Ile Arg Glu Ser Ala Val Thr Leu Lys Glu Ala
85 90 95
Glu Asp Ala Val Leu Ala Leu Ile Glu Lys His Cys Asp Pro Ala His
100 105 110
Pro Ala Pro Leu Ala Gly Asn Ser Ile Ala Thr Asp Arg Ala Phe Ile
115 120 125
Arg Glu His Met Pro Arg Leu Asp Glu Ala Leu His Tyr Arg Met Val
130 135 140
Asp Val Ser Ser Val Lys Glu Leu Ala Arg Arg Trp Tyr Pro Arg Val
145 150 155 160
Tyr Tyr Lys Gln Pro Glu Lys Gly Leu Ala His Arg Ala Leu Ala Asp
165 170 175
Ile Val Glu Ser Ile Arg Glu Leu Asp Tyr Tyr Arg Arg Ser Phe Phe
180 185 190
Val Ala Glu Pro Gly Pro Thr Ser Glu Gln Cys Ala Asp Asp Ala Gln
195 200 205
Ala Ala Val Asp Arg Phe Ala Pro Tyr Phe Asp
210 215
<210>223
<211>1288
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1258)
<223>RXA01982
<400>223
tgccgccgac catgagatga tcaagcgctt tttcaccctg caccttcgct aagactctac 60
cttcacccaa ctcatcatct actgaaagaa ggcttctgct atg gct acc acc agc 115
Met Ala Thr Thr Ser
1 5
ctc ccc acg acg att aca ccg gat tcc gag gag acc gta gaa aag cgt 163
Leu Pro Thr Thr Ile Thr Pro Asp Ser Glu Glu Thr Val Glu Lys Arg
10 15 20
cac tcc ctg acc ccg atc ctg gat tca ctt ccc tgc gag tca gtt ccc 211
His Ser Leu Thr Pro Ile Leu Asp Ser Leu Pro Cys Glu Ser Val Pro
25 30 35
tat gct ctg gct gca ggt gag gga caa caa cac cag ctt ggc ccc tac 259
Tyr Ala Leu Ala Ala Gly Glu Gly Gln Gln His Gln Leu Gly Pro Tyr
40 45 50
cat ctc aca gtt atg tcc cgt ccc gaa gac aac ggt gga ata ttt tcg 307
His Leu Thr Val Met Ser Arg Pro Glu Asp Asn Gly Gly Ile Phe Ser
55 60 65
ctg gct cgt gtg agc gca ggc aag act ccc gcc acc cgg ttc ttc tct 355
Leu Ala Arg Val Ser Ala Gly Lys Thr Pro Ala Thr Arg Phe Phe Ser
70 75 80 85
gtc gca gga ccg acg ttc ctc tac atg atg gag ggg cgg ttg act ctc 403
Val Ala Gly Pro Thr Phe Leu Tyr Met Met Glu Gly Arg Leu Thr Leu
90 95 100
tgg ttc gct gat gga cgt caa gag atc atc gct ggc ggc agt gcc acc 451
Trp Phe Ala Asp Gly Arg Gln Glu Ile Ile Ala Gly Gly Ser Ala Thr
105 110 115
att cct acg aat acg ccc tgg tcc ttc gcc tgt gag ggc cta gta aat 499
Ile Pro Thr Asn Thr Pro Trp Ser Phe Ala Cys Glu Gly Leu Val Asn
120 125 130
tca gct cta gtg tac tac tca tcc tcg aat gca ttc cta cac gcg gca 547
Ser Ala Leu Val Tyr Tyr Ser Ser Ser Asn Ala Phe Leu His Ala Ala
135 140 145
gag aaa ctt ggc acg tcc tcg ttc tcc cac acc ttc cgc gtc agt ggc 595
Glu Lys Leu Gly Thr Ser Ser Phe Ser His Thr Phe Arg Val Ser Gly
150 155 160 165
gag gtc aca ggg att ccc tac gag gaa ctg gag gat tgc gga ttc aca 643
Glu Val Thr Gly Ile Pro Tyr Glu Glu Leu Glu Asp Cys Gly Phe Thr
170 175 180
ttc tat gag cgg gac cac ctt gcc gag ctc ggg ccg cat ttc gat cgc 691
Phe Tyr Glu Arg Asp His Leu Ala Glu Leu Gly Pro His Phe Asp Arg
185 190 195
ctc cca gaa gat atg aag gcg ttt gcc ctg aac gat ggt gag ggg gac 739
Leu Pro Glu Asp Met Lys Ala Phe Ala Leu Asn Asp Gly Glu Gly Asp
200 205 210
cgg ctt gag cag ttc gag cag atc aac agc ttc gtc tgc cgt ccg aga 787
Arg Leu Glu Gln Phe Glu Gln Ile Asn Ser Phe Val Cys Arg Pro Arg
215 220 225
cac acc gga aat cag ttc ctc gct atg cag acc aag ggt gcc aag acg 835
His Thr Gly Asn Gln Phe Leu Ala Met Gln Thr Lys Gly Ala Lys Thr
230 235 240 245
cct tat ata cct cgt cat ttc cac cgc ctt cac acc gaa aac ttc atc 883
Pro Tyr Ile Pro Arg His Phe His Arg Leu His Thr Glu Asn Phe Ile
250 255 260
tgt ctg gac ggg cgc gtc aaa ctg cac gtc aac ggt caa gag atc atc 931
Cys Leu Asp Gly Arg Val Lys Leu His Val Asn Gly Gln Glu Ile Ile
265 270 275
ctc tcc cgt ggg gat tac gtt cat gcc cca gcc gga acc atc cac tct 979
Leu Ser Arg Gly Asp Tyr Val His Ala Pro Ala Gly Thr Ile His Ser
280 285 290
ttc gcc ttc gca ggg cac aat acc cag atg ctg gga gtg ctc acc aca 1027
Phe Ala Phe Ala Gly His Asn Thr Gln Met Leu Gly Val Leu Thr Thr
295 300 305
gag gta ttc gag aag ttc ttt gac tac ata aac act ccg act aat gct 1075
Glu Val Phe Glu Lys Phe Phe Asp Tyr Ile Asn Thr Pro Thr Asn Ala
310 315 320 325
cga gtt cag ctg gag gac ggg ggg aag cca ttt ttt cct gct gaa gca 1123
Arg Val Gln Leu Glu Asp Gly Gly Lys Pro Phe Phe Pro Ala Glu Ala
330 335 340
ttc gcg aaa gtc caa gct gaa ctc gat gtc gtg gtg gtc ggc ccc cca 1171
Phe Ala Lys Val Gln Ala Glu Leu Asp Val Val Val Val Gly Pro Pro
345 350 355
cga att agt acc ctg gat gtt gtt aca aac tcg cgt ttc gaa cca att 1219
Arg Ile Ser Thr Leu Asp Val Val Thr Asn Ser Arg Phe Glu Pro Ile
360 365 370
aac aga act atc acc tat cgt gcg ctg gac gct aac caa tagtgagtga 1268
Asn Arg Thr Ile Thr Tyr Arg Ala Leu Asp Ala Asn Gln
375 380 385
gccgaaatcc ctgtttgtaa 1288
<210>224
<211>386
<212>PRT
<213>谷氨酸棒状杆菌
<400>224
Met Ala Thr Thr Ser Leu Pro Thr Thr Ile Thr Pro Asp Ser Glu Glu
1 5 10 15
Thr Val Glu Lys Arg His Ser Leu Thr Pro Ile Leu Asp Ser Leu Pro
20 25 30
Cys Glu Ser Val Pro Tyr Ala Leu Ala Ala Gly Glu Gly Gln Gln His
35 40 45
Gln Leu Gly Pro Tyr His Leu Thr Val Met Ser Arg Pro Glu Asp Asn
50 55 60
Gly Gly Ile Phe Ser Leu Ala Arg Val Ser Ala Gly Lys Thr Pro Ala
65 70 75 80
Thr Arg Phe Phe Ser Val Ala Gly Pro Thr Phe Leu Tyr Met Met Glu
85 90 95
Gly Arg Leu Thr Leu Trp Phe Ala Asp Gly Arg Gln Glu Ile Ile Ala
100 105 110
Gly Gly Ser Ala Thr Ile Pro Thr Asn Thr Pro Trp Ser Phe Ala Cys
115 120 125
Glu Gly Leu Val Asn Ser Ala Leu Val Tyr Tyr Ser Ser Ser Asn Ala
130 135 140
Phe Leu His Ala Ala Glu Lys Leu Gly Thr Ser Ser Phe Ser His Thr
145 150 155 160
Phe Arg Val Ser Gly Glu Val Thr Gly Ile Pro Tyr Glu Glu Leu Glu
165 170 175
Asp Cys Gly Phe Thr Phe Tyr Glu Arg Asp His Leu Ala Glu Leu Gly
180 185 190
Pro His Phe Asp Arg Leu Pro Glu Asp Met Lys Ala Phe Ala Leu Asn
195 200 205
Asp Gly Glu Gly Asp Arg Leu Glu Gln Phe Glu Gln Ile Asn Ser Phe
210 215 220
Val Cys Arg Pro Arg His Thr Gly Asn Gln Phe Leu Ala Met Gln Thr
225 230 235 240
Lys Gly Ala Lys Thr Pro Tyr Ile Pro Arg His Phe His Arg Leu His
245 250 255
Thr Glu Asn Phe Ile Cys Leu Asp Gly Arg Val Lys Leu His Val Asn
260 265 270
Gly Gln Glu Ile Ile Leu Ser Arg Gly Asp Tyr Val His Ala Pro Ala
275 280 285
Gly Thr Ile His Ser Phe Ala Phe Ala Gly His Asn Thr Gln Met Leu
290 295 300
Gly Val Leu Thr Thr Glu Val Phe Glu Lys Phe Phe Asp Tyr Ile Asn
305 310 315 320
Thr Pro Thr Asn Ala Arg Val Gln Leu Glu Asp Gly Gly Lys Pro Phe
325 330 335
Phe Pro Ala Glu Ala Phe Ala Lys Val Gln Ala Glu Leu Asp Val Val
340 345 350
Val Val Gly Pro Pro Arg Ile Ser Thr Leu Asp Val Val Thr Asn Ser
355 360 365
Arg Phe Glu Pro Ile Asn Arg Thr Ile Thr Tyr Arg Ala Leu Asp Ala
370 375 380
Asn Gln
385
<210>225
<211>1078
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1048)
<223>RXA02021
<400>225
ttgggtcgcc gaggagatct aatcctggtt tgagttcaga gttcacaggt ttaagcctac 60
aaaccttagt taaaacatga tggaagcggt cgattaaaaa atg agt gaa aac att 115
Met Ser Glu Asn Ile
1 5
cgc gga gcc caa gca gtt gga atc gca aat atc gcc atg gac ggg acc 163
Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile Ala Met Asp Gly Thr
10 15 20
atc ctg gac acg tgg tac cca gaa ccc caa att ttc aac ccg gat cag 211
Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile Phe Asn Pro Asp Gln
25 30 35
tgg gct gaa cgc tac cca ttg gaa gtg ggc acc aca cgc ctc gga gca 259
Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr Thr Arg Leu Gly Ala
40 45 50
aac gaa ctc acc cca cgg atg ctg cag ttg gta aaa ctg gac caa gat 307
Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val Lys Leu Asp Gln Asp
55 60 65
cgc ctc gtc gaa cag gta gca gtc cgc acc gtt atc ccc gat ctg tct 355
Arg Leu Val Glu Gln Val Ala Val Arg Thr Val Ile Pro Asp Leu Ser
70 75 80 85
caa cct cca gta gac gcg cac gat gtt tac ctg cgc ctc cac ctg ctt 403
Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu Arg Leu His Leu Leu
90 95 100
tcc cac cgc ctg gtc cgc ccc cac gaa atg cac atg caa aac acc ttg 451
Ser His Arg Leu Val Arg Pro His Glu Met His Met Gln Asn Thr Leu
105 110 115
gag ctg ctg tcc gac gtg gtg tgg aca aac aag ggc cct tgc ctt cct 499
Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys Gly Pro Cys Leu Pro
120 125 130
gaa aac ttt gag tgg gtg cgt ggt gct ctg cgg tcc cgc gga ctc atc 547
Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg Ser Arg Gly Leu Ile
135 140 145
cac gtc tac tgt gtg gac cgt ctt ccc cgc atg gtc gac tat gtg gtt 595
His Val Tyr Cys Val Asp Arg Leu Pro Arg Met Val Asp Tyr Val Val
150 155 160 165
ccc cct gga gtc cgc atc tcc gaa gca gaa cgc gtg cgc cta ggt gca 643
Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg Val Arg Leu Gly Ala
170 175 180
tac ctt gct ccg ggt acc tct gtg ctg cgt gaa ggt ttc gtg tct ttc 691
Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu Gly Phe Val Ser Phe
185 190 195
aac tcc ggc acc ttg ggt gcc gca aag gtg gaa ggc cgc ctg agt tcc 739
Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu Gly Arg Leu Ser Ser
200 205 210
ggt gtg gtc atc ggt gaa ggt tcc gag att gga ctg tct tct act att 787
Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly Leu Ser Ser Thr Ile
215 220 225
cag tcc ccg aga gat gaa cag cgc cgc cgt ttg ccg ttg agc atc ggc 835
Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu Pro Leu Ser Ile Gly
230 235 240 245
caa aac tgc aac ttt ggt gtc agc tcc gga atc atc gga gtc agt ctg 883
Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile Ile Gly Val Ser Leu
250 255 260
gga gac aat tgc gac atc gga aat aac att gtc ttg gat gga gat acc 931
Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val Leu Asp Gly Asp Thr
265 270 275
ccc att tgg ttc gca gcc gat gag gag tta cgc act atc gac tcc atc 979
Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg Thr Ile Asp Ser Ile
280 285 290
gaa ggc caa gca aat tgg tca atc aag cgt gaa tcc ggc ttc cat gag 1027
Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu Ser Gly Phe His Glu
295 300 305
cca gtt gcc cgc ctc aaa gct tgacccattt tcataaccag tgccatgtgg 1078
Pro Val Ala Arg Leu Lys Ala
310 315
<210>226
<211>316
<212>PRT
<213>谷氨酸棒状杆菌
<400>226
Met Ser Glu Asn Ile Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile
1 5 10 15
Ala Met Asp Gly Thr Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile
20 25 30
Phe Asn Pro Asp Gln Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr
35 40 45
Thr Arg Leu Gly Ala Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val
50 55 60
Lys Leu Asp Gln Asp Arg Leu Val Glu Gln Val Ala Val Arg Thr Val
65 70 75 80
Ile Pro Asp Leu Ser Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu
85 90 95
Arg Leu His Leu Leu Ser His Arg Leu Val Arg Pro His Glu Met His
100 105 110
Met Gln Asn Thr Leu Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys
115 120 125
Gly Pro Cys Leu Pro Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg
130 135 140
Ser Arg Gly Leu Ile His Val Tyr Cys Val Asp Arg Leu Pro Arg Met
145 150 155 160
Val Asp Tyr Val Val Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg
165 170 175
Val Arg Leu Gly Ala Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu
180 185 190
Gly Phe Val Ser Phe Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu
195 200 205
Gly Arg Leu Ser Ser Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly
210 215 220
Leu Ser Ser Thr Ile Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu
225 230 235 240
Pro Leu Ser Ile Gly Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile
245 250 255
Ile Gly Val Ser Leu Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val
260 265 270
Leu Asp Gly Asp Thr Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg
275 280 285
Thr Ile Asp Ser Ile Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu
290 295 300
Ser Gly Phe His Glu Pro Val Ala Arg Leu Lys Ala
305 310 315
<210>227
<211>829
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(799)
<223>RXA02032
<400>227
tggcgcaacc acttccattt cgaaggggag tagttccaca caaaggcgat ctgactcgtt 60
tttgtttgag gtatgttcga catactggag caagttcacc ttg cac ccg gca tac 115
Leu His Pro Ala Tyr
1 5
cac ctg aac aag gcc tta aaa agg ggc ttt atc ggt tca ggc gag cga 163
His Leu Asn Lys Ala Leu Lys Arg Gly Phe Ile Gly Ser Gly Glu Arg
10 15 20
gac ctt cgg cgt tca cac ttc gcc ggg gtc tat ttt tta tgc cca ggc 211
Asp Leu Arg Arg Ser His Phe Ala Gly Val Tyr Phe Leu Cys Pro Gly
25 30 35
acg gct cat agg aga acc atg ccc ttt cta caa atc tct ctg ctt tcc 259
Thr Ala His Arg Arg Thr Met Pro Phe Leu Gln Ile Ser Leu Leu Ser
40 45 50
atc ggt gtc gcc gcc gat gcg ttt gct tgt tcc gtt gtc cgc ggc acc 307
Ile Gly Val Ala Ala Asp Ala Phe Ala Cys Ser Val Val Arg Gly Thr
55 60 65
gcc att caa gtc aac ctt ttc aaa cgc gca ctt gtt ctc gcg ggc atc 355
Ala Ile Gln Val Asn Leu Phe Lys Arg Ala Leu Val Leu Ala Gly Ile
70 75 80 85
ttt ggt gtc ttc caa gcg gca atg cct tta atc ggc tgg ttc att ggc 403
Phe Gly Val Phe Gln Ala Ala Met Pro Leu Ile Gly Trp Phe Ile Gly
90 95 100
cgt ttc ttt gct gga atc acc ttc atc gct gaa att gat cac tgg atc 451
Arg Phe Phe Ala Gly Ile Thr Phe Ile Ala Glu Ile Asp His Trp Ile
105 110 115
gct ttt gca cta ttg ggt att gtc ggc acc aaa atg atc tgg gat gcc 499
Ala Phe Ala Leu Leu Gly Ile Val Gly Thr Lys Met Ile Trp Asp Ala
120 125 130
ttc caa cct gaa gat gat gaa acc att gtc gat gac ggc cgc gtt caa 547
Phe Gln Pro Glu Asp Asp Glu Thr Ile Val Asp Asp Gly Arg Val Gln
135 140 145
ttt aga cca gca att atc ctg ggg cta gcc acc agc att gac gca tta 595
Phe Arg Pro Ala Ile Ile Leu Gly Leu Ala Thr Ser Ile Asp Ala Leu
150 155 160 165
gcc gta ggc atg ggc ctg gca ttc gtg gaa gtt tcc atc ctc aaa gtg 643
Ala Val Gly Met Gly Leu Ala Phe Val Glu Val Ser Ile Leu Lys Val
170 175 180
gca ctg tcc atg ggc agc atc acc ttc gca ctt tcg ctt gct ggc gcc 691
Ala Leu Ser Met Gly Ser Ile Thr Phe Ala Leu Ser Leu Ala Gly Ala
185 190 195
tgg atc gga cac cat ggt gga gga aag ttt ggc aag tgg gct acg att 739
Trp Ile Gly His His Gly Gly Gly Lys Phe Gly Lys Trp Ala Thr Ile
200 205 210
ctt ggc gga ata atc ttg atc gga atc ggc gca aac atc gtc tac gaa 787
Leu Gly Gly Ile Ile Leu Ile Gly Ile Gly Ala Asn Ile Val Tyr Glu
215 220 225
cac ctc agc gcg taaccctcgg cgcattatcc tcagacagct 829
His Leu Ser Ala
230
<210>228
<211>233
<212>PRT
<213>谷氨酸棒状杆菌
<400>228
Leu His Pro Ala Tyr His Leu Asn Lys Ala Leu Lys Arg Gly Phe Ile
1 5 10 15
Gly Ser Gly Glu Arg Asp Leu Arg Arg Ser His Phe Ala Gly Val Tyr
20 25 30
Phe Leu Cys Pro Gly Thr Ala His Arg Arg Thr Met Pro Phe Leu Gln
35 40 45
Ile Ser Leu Leu Ser Ile Gly Val Ala Ala Asp Ala Phe Ala Cys Ser
50 55 60
Val Val Arg Gly Thr Ala Ile Gln Val Asn Leu Phe Lys Arg Ala Leu
65 70 75 80
Val Leu Ala Gly Ile Phe Gly Val Phe Gln Ala Ala Met Pro Leu Ile
85 90 95
Gly Trp Phe Ile Gly Arg Phe Phe Ala Gly Ile Thr Phe Ile Ala Glu
100 105 110
Ile Asp His Trp Ile Ala Phe Ala Leu Leu Gly Ile Val Gly Thr Lys
115 120 125
Met Ile Trp Asp Ala Phe Gln Pro Glu Asp Asp Glu Thr Ile Val Asp
130 135 140
Asp Gly Arg Val Gln Phe Arg Pro Ala Ile Ile Leu Gly Leu Ala Thr
145 150 155 160
Ser Ile Asp Ala Leu Ala Val Gly Met Gly Leu Ala Phe Val Glu Val
165 170 175
Ser Ile Leu Lys Val Ala Leu Ser Met Gly Ser Ile Thr Phe Ala Leu
180 185 190
Ser Leu Ala Gly Ala Trp Ile Gly His His Gly Gly Gly Lys Phe Gly
195 200 205
Lys Trp Ala Thr Ile Leu Gly Gly Ile Ile Leu Ile Gly Ile Gly Ala
210 215 220
Asn Ile Val Tyr Glu His Leu Ser Ala
225 230
<210>229
<211>709
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(679)
<223>RXA02053
<400>229
aaccagccag aaactatctc caaaagctaa taaaaccctt gcactgacaa ataaggcgac 60
ctaccatgac tctgtttcca acacataaaa aggataaaaa atg tca ctt tca gtc 115
Met Ser Leu Ser Val
1 5
gtc gag gcg att acc aac cgc cgc gcc acc cgc aaa tac acc gat gaa 163
Val Glu Ala Ile Thr Asn Arg Arg Ala Thr Arg Lys Tyr Thr Asp Glu
10 15 20
gct cct acc cct gag ctg atc gac aaa atc gtt gac ctt gcc ctg gag 211
Ala Pro Thr Pro Glu Leu Ile Asp Lys Ile Val Asp Leu Ala Leu Glu
25 30 35
gca ccc agt gcg ttc aat gcg cag caa cgt gaa att gtt gtg att act 259
Ala Pro Ser Ala Phe Asn Ala Gln Gln Arg Glu Ile Val Val Ile Thr
40 45 50
gat ccc gca cag aag cag aag ctt tac gag gcc tcc cat cag aaa caa 307
Asp Pro Ala Gln Lys Gln Lys Leu Tyr Glu Ala Ser His Gln Lys Gln
55 60 65
ttc ctc acc gca cct gta act ttc att gcg gtt gcc cgc gtg gaa aac 355
Phe Leu Thr Ala Pro Val Thr Phe Ile Ala Val Ala Arg Val Glu Asn
70 75 80 85
gag cct gag gat ttg gaa gag att ctt ggt acg gaa agg gct gaa cgt 403
Glu Pro Glu Asp Leu Glu Glu Ile Leu Gly Thr Glu Arg Ala Glu Arg
90 95 100
gtc gcg gga ttc atc aac ggt cgc agc att cag cag gca cgc gaa gca 451
Val Ala Gly Phe Ile Asn Gly Arg Ser Ile Gln Gln Ala Arg Glu Ala
105 110 115
acg ttg agg gat gcc agc ctc gcg gcg gct ttt cta att ctg gct gcc 499
Thr Leu Arg Asp Ala Ser Leu Ala Ala Ala Phe Leu Ile Leu Ala Ala
120 125 130
cag gcg gag ggt ttg agt acc agc ccg act act ggt tgg gat gag gaa 547
Gln Ala Glu Gly Leu Ser Thr Ser Pro Thr Thr Gly Trp Asp Glu Glu
135 140 145
aaa gtg aag gaa gca atc ggt ctc ggc ggg cgt gag gat cgt gca atc 595
Lys Val Lys Glu Ala Ile Gly Leu Gly Gly Arg Glu Asp Arg Ala Ile
150 155 160 165
gcc ctt gtt att gct acc gga ttc cct aat gaa cag ccg gag cac cct 643
Ala Leu Val Ile Ala Thr Gly Phe Pro Asn Glu Gln Pro Glu His Pro
170 175 180
ggt cgt ttg cag aat agg cgc atc gac aac agc tac taactctgcc 689
Gly Arg Leu Gln Asn Arg Arg Ile Asp Asn Ser Tyr
185 190
agctcgcccg gacgaactaa 709
<210>230
<211>193
<212>PRT
<213>谷氨酸棒状杆菌
<400>230
Met Ser Leu Ser Val Val Glu Ala Ile Thr Asn Arg Arg Ala Thr Arg
1 5 10 15
Lys Tyr Thr Asp Glu Ala Pro Thr Pro Glu Leu Ile Asp Lys Ile Val
20 25 30
Asp Leu Ala Leu Glu Ala Pro Ser Ala Phe Asn Ala Gln Gln Arg Glu
35 40 45
Ile Val Val Ile Thr Asp Pro Ala Gln Lys Gln Lys Leu Tyr Glu Ala
50 55 60
Ser His Gln Lys Gln Phe Leu Thr Ala Pro Val Thr Phe Ile Ala Val
65 70 75 80
Ala Arg Val Glu Asn Glu Pro Glu Asp Leu Glu Glu Ile Leu Gly Thr
85 90 95
Glu Arg Ala Glu Arg Val Ala Gly Phe Ile Asn Gly Arg Ser Ile Gln
100 105 110
Gln Ala Arg Glu Ala Thr Leu Arg Asp Ala Ser Leu Ala Ala Ala Phe
115 120 125
Leu Ile Leu Ala Ala Gln Ala Glu Gly Leu Ser Thr Ser Pro Thr Thr
130 135 140
Gly Trp Asp Glu Glu Lys Val Lys Glu Ala Ile Gly Leu Gly Gly Arg
145 150 155 160
Glu Asp Arg Ala Ile Ala Leu Val Ile Ala Thr Gly Phe Pro Asn Glu
165 170 175
Gln Pro Glu His Pro Gly Arg Leu Gln Asn Arg Arg Ile Asp Asn Ser
180 185 190
Tyr
<210>231
<211>796
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(766)
<223>RXA02058
<400>231
gcgctatgat gtgatttgtt ccccagaacg tgctcgggaa gctcgagaca tgattgccct 60
gaaaactcga gatctccgcc aataagttaa actaacgcct atg aaa aat gtg ggg 115
Met Lys Asn Val Gly
1 5
aag cgg att tta gtc ctt gcg ctt ggt gcc tcg gta gcg gga tgc tca 163
Lys Arg Ile Leu Val Leu Ala Leu Gly Ala Ser Val Ala Gly Cys Ser
10 15 20
acg ctt tcc caa gaa cct tca cca cct gtt ccg ttg gga aac gtt gat 211
Thr Leu Ser Gln Glu Pro Ser Pro Pro Val Pro Leu Gly Asn Val Asp
25 30 35
act gtg cag att gtc tct ccc aat ggg gag att gag tct ttt gtg ctg 259
Thr Val Gln Ile Val Ser Pro Asn Gly Glu Ile Glu Ser Phe Val Leu
40 45 50
ggg aag ctg tat gag acc gca tta gtg gaa cgt ggc aga tct gca tca 307
Gly Lys Leu Tyr Glu Thr Ala Leu Val Glu Arg Gly Arg Ser Ala Ser
55 60 65
gtt cag ctg att gac ggc gac ttg gat gag caa cta tct atg ctg cga 355
Val Gln Leu Ile Asp Gly Asp Leu Asp Glu Gln Leu Ser Met Leu Arg
70 75 80 85
gat gac agc act gat ttg gtg att gct tgc tca gga caa tta ctg gaa 403
Asp Asp Ser Thr Asp Leu Val Ile Ala Cys Ser Gly Gln Leu Leu Glu
90 95 100
tat tac aac cca gat tta gcc tcc gag ttt gcc gtc gaa tac gct aat 451
Tyr Tyr Asn Pro Asp Leu Ala Ser Glu Phe Ala Val Glu Tyr Ala Asn
105 110 115
cag aca gcc ttt gat aaa aac tct ggt gaa tgg cgc gaa aaa gtc tac 499
Gln Thr Ala Phe Asp Lys Asn Ser Gly Glu Trp Arg Glu Lys Val Tyr
120 125 130
gat gct ctc caa gga tcg ctg ccg gac tcc atc gtg gcc acc gat cct 547
Asp Ala Leu Gln Gly Ser Leu Pro Asp Ser Ile Val Ala Thr Asp Pro
135 140 145
tcc aat gct att ggt tgt aag gac gat acg tcg ctg cct caa aac atc 595
Ser Asn Ala Ile Gly Cys Lys Asp Asp Thr Ser Leu Pro Gln Asn Ile
150 155 160 165
gtg cca att tat aga aag ccc aat ctg gat agg gac aat cgg gac acc 643
Val Pro Ile Tyr Arg Lys Pro Asn Leu Asp Arg Asp Asn Arg Asp Thr
170 175 180
ctg aac ttt gtg agc ggt tct ttg ggt aca agc gat ttg gaa gca ttg 691
Leu Asn Phe Val Ser Gly Ser Leu Gly Thr Ser Asp Leu Glu Ala Lau
185 190 195
gtc aag gac gcc caa aca aca ggc aca acc tct gaa act gcg ctg gat 739
Val Lys Asp Ala Gln Thr Thr Gly Thr Thr Ser Glu Thr Ala Leu Asp
200 205 210
ttc tta ttg tct aaa gga ttt tca cgc tagttcagcc gtggtgatgc 786
Phe Leu Leu Ser Lys Gly Phe Ser Arg
215 220
acgtgggggc 796
<210>232
<211>222
<212>PRT
<213>谷氨酸棒状杆菌
<400>232
Met Lys Asn Val Gly Lys Arg Ile Leu Val Leu Ala Leu Gly Ala Ser
1 5 10 15
Val Ala Gly Cys Ser Thr Leu Ser Gln Glu Pro Ser Pro Pro Val Pro
20 25 30
Leu Gly Asn Val Asp Thr Val Gln Ile Val Ser Pro Asn Gly Glu Ile
35 40 45
Glu Ser Phe Val Leu Gly Lys Leu Tyr Glu Thr Ala Leu Val Glu Arg
50 55 60
Gly Arg Ser Ala Ser Val Gln Leu Ile Asp Gly Asp Leu Asp Glu Gln
65 70 75 80
Leu Ser Met Leu Arg Asp Asp Ser Thr Asp Leu Val Ile Ala Cys Ser
85 90 95
Gly Gln Leu Leu Glu Tyr Tyr Asn Pro Asp Leu Ala Ser Glu Phe Ala
100 105 110
Val Glu Tyr Ala Asn Gln Thr Ala Phe Asp Lys Asn Ser Gly Glu Trp
115 120 125
Arg Glu Lys Val Tyr Asp Ala Leu Gln Gly Ser Leu Pro Asp Ser Ile
130 135 140
Val Ala Thr Asp Pro Ser Asn Ala Ile Gly Cys Lys Asp Asp Thr Ser
145 150 155 160
Leu Pro Gln Asn Ile Val Pro Ile Tyr Arg Lys Pro Asn Leu Asp Arg
165 170 175
Asp Asn Arg Asp Thr Leu Asn Phe Val Ser Gly Ser Leu Gly Thr Ser
180 185 190
Asp Leu Glu Ala Leu Val Lys Asp Ala Gln Thr Thr Gly Thr Thr Ser
195 200 205
Glu Thr Ala Leu Asp Phe Leu Leu Ser Lys Gly Phe Ser Arg
210 215 220
<210>233
<211>625
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(595)
<223>RXA02059
<400>233
tcgggaaatt ccactcatga acgcctagtc tacgggaacc atttcaccag cgtgtacgtt 60
gtaaatgtga actgaaaaag tgaaaaggaa gatgataaag atg act caa cca cgg 115
Met Thr Gln Pro Arg
1 5
ccc gat gcc gca tct gtg tcg ctg gaa aag aag cgc cca gaa gga tgg 163
Pro Asp Ala Ala Ser Val Ser Leu Glu Lys Lys Arg Pro Glu Gly Trp
10 15 20
cca gtg gga agc ttt gaa aca tac cca gaa gcc caa gca gca gtg gat 211
Pro Val Gly Ser Phe Glu Thr Tyr Pro Glu Ala Gln Ala Ala Val Asp
25 30 35
ttg ctc agt gat aat gca ttc ccc gtc acc gaa ttg acc att gtt ggt 259
Leu Leu Ser Asp Asn Ala Phe Pro Val Thr Glu Leu Thr Ile Val Gly
40 45 50
gtg gac ctg att gaa gtg gaa cgc gtt aca ggt cgt ctc acg tgg ggt 307
Val Asp Leu Ile Glu Val Glu Arg Val Thr Gly Arg Leu Thr Trp Gly
55 60 65
cgt gtg att gcc gga gga atg gca tct ggc gca tgg ttg ggt ctg ttc 355
Arg Val Ile Ala Gly Gly Met Ala Ser Gly Ala Trp Leu Gly Leu Phe
70 75 80 85
ttt ggc att gtc atg gcc ttg atg tct gga ttc tgg ttc tct tcc atc 403
Phe Gly Ile Val Met Ala Leu Met Ser Gly Phe Trp Phe Ser Ser Ile
90 95 100
gca gcg gga ata ggt atg ggt ttg gtg ttt ggc att gtc ggt gca gca 451
Ala Ala Gly Ile Gly Met Gly Leu Val Phe Gly Ile Val Gly Ala Ala
105 110 115
gtt cct tat gct gct tcc aaa ggc aag cgg gac ttt acc tct tca act 499
Val Pro Tyr Ala Ala Ser Lys Gly Lys Arg Asp Phe Thr Ser Ser Thr
120 125 130
caa att gtg gcg ggg cgc tat gat gtg att tgt tcc cca gaa cgt gct 547
Gln Ile Val Ala Gly Arg Tyr Asp Val Ile Cys Ser Pro Glu Arg Ala
135 140 145
cgg gaa gct cga gac atg att gcc ctg aaa act cga gat ctc cgc caa 595
Arg Glu Ala Arg Asp Met Ile Ala Leu Lys Thr Arg Asp Leu Arg Gln
150 155 160 165
taagttaaac taacgcctat gaaaaatgtg 625
<210>234
<211>165
<212>PRT
<213>谷氨酸棒状杆菌
<400>234
Met Thr Gln Pro Arg Pro Asp Ala Ala Ser Val Ser Leu Glu Lys Lys
1 5 10 15
Arg Pro Glu Gly Trp Pro Val Gly Ser Phe Glu Thr Tyr Pro Glu Ala
20 25 30
Gln Ala Ala Val Asp Leu Leu Ser Asp Asn Ala Phe Pro Val Thr Glu
35 40 45
Leu Thr Ile Val Gly Val Asp Leu Ile Glu Val Glu Arg Val Thr Gly
50 55 60
Arg Leu Thr Trp Gly Arg Val Ile Ala Gly Gly Met Ala Ser Gly Ala
65 70 75 80
Trp Leu Gly Leu Phe Phe Gly Ile Val Met Ala Leu Met Ser Gly Phe
85 90 95
Trp Phe Ser Ser Ile Ala Ala Gly Ile Gly Met Gly Leu Val Phe Gly
100 105 110
Ile Val Gly Ala Ala Val Pro Tyr Ala Ala Ser Lys Gly Lys Arg Asp
115 120 125
Phe Thr Ser Ser Thr Gln Ile Val Ala Gly Arg Tyr Asp Val Ile Cys
130 135 140
Ser Pro Glu Arg Ala Arg Glu Ala Arg Asp Met Ile Ala Leu Lys Thr
145 150 155 160
Arg Asp Leu Arg Gln
165
<210>235
<211>1255
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1225)
<223>RXA02070
<400>235
aacagcacgg atcaggacct caagatttgt ctgaccccac tcagggtgat gttgcagacg 60
aattttaggg agtatcaaaa cttgggacta ctatcgatac gtg atg act caa gta 115
Val Met Thr Gln Val
1 5
acc gaa tcc gct gtc cgc agc gcg cta tcc cgc gta gag gat cca gag 163
Thr Glu Ser Ala Val Arg Ser Ala Leu Ser Arg Val Glu Asp Pro Glu
10 15 20
atc ggt aag ccc atc aca gag ctc ggc atg gtc aaa tca gtg tcc atc 211
Ile Gly Lys Pro Ile Thr Glu Leu Gly Met Val Lys Ser Val Ser Ile
25 30 35
gac ggc tct gat gtc cag gtg gag gtc tac ctg acg atc gcg gct tgc 259
Asp Gly Ser Asp Val Gln Val Glu Val Tyr Leu Thr Ile Ala Ala Cys
40 45 50
ccg atg aaa acc acc att gtc acc aac act gaa gca gct ctc aaa gac 307
Pro Met Lys Thr Thr Ile Val Thr Asn Thr Glu Ala Ala Leu Lys Asp
55 60 65
atc gac ggg gtt ggc caa gtt cat gtc acc acc gat gtc atg agt gat 355
Ile Asp Gly Val Gly Gln Val His Val Thr Thr Asp Val Met Ser Asp
70 75 80 85
gaa cag cgc cgt gcg ctc cgc gtc tcc ctg cgc ggt gaa act tct gag 403
Glu Gln Arg Arg Ala Leu Arg Val Ser Leu Arg Gly Glu Thr Ser Glu
90 95 100
cca gtg att cca ttc gct cag cct ggt tcc act acc cgc gtt tac gct 451
Pro Val Ile Pro Phe Ala Gln Pro Gly Ser Thr Thr Arg Val Tyr Ala
105 110 115
gtt gct tcc ggc aaa ggt ggc gta gga aaa tcc tcc atg acg gtg aac 499
Val Ala Ser Gly Lys Gly Gly Val Gly Lys Ser Ser Met Thr Val Asn
120 125 130
ttg gct gca gcc cta gcc aag cgc ggg ctg tct gtg gga att ttg gat 547
Leu Ala Ala Ala Leu Ala Lys Arg Gly Leu Ser Val Gly Ile Leu Asp
135 140 145
gcc gat att tac gga cac tca gtg ccc gga atg ctc ggc tcg gac caa 595
Ala Asp Ile Tyr Gly His Ser Val Pro Gly Met Leu Gly Ser Asp Gln
150 155 160 165
cgc cca cac cag gtc gat gac atg atc atg cct ccc cag gcg cac ggc 643
Arg Pro His Gln Val Asp Asp Met Ile Met Pro Pro Gln Ala His Gly
170 175 180
gtg aag atg ata tcc att gct cac ttc acc gaa gga aat gct cct gtg 691
Val Lys Met Ile Ser Ile Ala His Phe Thr Glu Gly Asn Ala Pro Val
185 190 195
gtg tgg cgt gga cca atg ctg cac cgt gcc atc cag caa ttc ctc act 739
Val Trp Arg Gly Pro Met Leu His Arg Ala Ile Gln Gln Phe Leu Thr
200 205 210
gac gtg ttc tgg ggc gac ctg gat att ttg ctg ctg gat ctt cct cca 787
Asp Val Phe Trp Gly Asp Leu Asp Ile Leu Leu Leu Asp Leu Pro Pro
215 220 225
gga act ggt gac atc gcc atc acc gtt gcc caa ttg atc ccg aat gct 835
Gly Thr Gly Asp Ile Ala Ile Thr Val Ala Gln Leu Ile Pro Asn Ala
230 235 240 245
gag ttg ctc att gtg acc act cct cag gct gcc gca gct gag gtt gcc 883
Glu Leu Leu Ile Val Thr Thr Pro Gln Ala Ala Ala Ala Glu Val Ala
250 255 260
gag cga gca gga acg atc tct gtg cag acc aac cag aag gtt gct ggc 931
Glu Arg Ala Gly Thr Ile Ser Val Gln Thr Asn Gln Lys Val Ala Gly
265 270 275
gtg att gaa aac atg tct gcc atg gtg ctt cct gat ggc acc acc atg 979
Val Ile Glu Asn Met Ser Ala Met Val Leu Pro Asp Gly Thr Thr Met
280 285 290
gat gtt ttc ggc acc ggc ggc ggt caa aag att gct gat cgt ctt acc 1027
Asp Val Phe Gly Thr Gly Gly Gly Gln Lys Ile Ala Asp Arg Leu Thr
295 300 305
gct gtg aca ggt gaa gag gtc aag gtt atc gga tct gtt cca ttg gat 1075
Ala Val Thr Gly Glu Glu Val Lys Val Ile Gly Ser Val Pro Leu Asp
310 315 320 325
ccg aac ctg cgt atc ggt ggc gat gtg gga aat cct att gcg att tct 1123
Pro Asn Leu Arg Ile Gly Gly Asp Val Gly Asn Pro Ile Ala Ile Ser
330 335 340
gaa cca cac tcc cca acc gct gca gcg atc aat gag atc gct gaa cac 1171
Glu Pro His Ser Pro Thr Ala Ala Ala Ile Asn Glu Ile Ala Glu His
345 350 355
cta gct cac cgc aag gta tcg ctg gtg ggt aaa acg ctt ggg ctt ggc 1219
Leu Ala His Arg Lys Val Ser Leu Val Gly Lys Thr Leu Gly Leu Gly
360 365 370
gtt aaa taaaagctaa ataatatcgg tccaagagac 1255
Val Lys
375
<210>236
<211>375
<212>PRT
<213>谷氨酸棒状杆菌
<400>236
Val Met Thr Gln Val Thr Glu Ser Ala Val Arg Ser Ala Leu Ser Arg
1 5 10 15
Val Glu Asp Pro Glu Ile Gly Lys Pro Ile Thr Glu Leu Gly Met Val
20 25 30
Lys Ser Val Ser Ile Asp Gly Ser Asp Val Gln Val Glu Val Tyr Leu
35 40 45
Thr Ile Ala Ala Cys Pro Met Lys Thr Thr Ile Val Thr Asn Thr Glu
50 55 60
Ala Ala Leu Lys Asp Ile Asp Gly Val Gly Gln Val His Val Thr Thr
65 70 75 80
Asp Val Met Ser Asp Glu Gln Arg Arg Ala Leu Arg Val Ser Leu Arg
85 90 95
Gly Glu Thr Ser Glu Pro Val Ile Pro Phe Ala Gln Pro Gly Ser Thr
100 105 110
Thr Arg Val Tyr Ala Val Ala Ser Gly Lys Gly Gly Val Gly Lys Ser
115 120 125
Ser Met Thr Val Asn Leu Ala Ala Ala Leu Ala Lys Arg Gly Leu Ser
130 135 140
Val Gly Ile Leu Asp Ala Asp Ile Tyr Gly His Ser Val Pro Gly Met
145 150 155 160
Leu Gly Ser Asp Gln Arg Pro His Gln Val Asp Asp Met Ile Met Pro
165 170 175
Pro Gln Ala His Gly Val Lys Met Ile Ser Ile Ala His Phe Thr Glu
180 185 190
Gly Asn Ala Pro Val Val Trp Arg Gly Pro Met Leu His Arg Ala Ile
195 200 205
Gln Gln Phe Leu Thr Asp Val Phe Trp Gly Asp Leu Asp Ile Leu Leu
210 215 220
Leu Asp Leu Pro Pro Gly Thr Gly Asp Ile Ala Ile Thr Val Ala Gln
225 230 235 240
Leu Ile Pro Asn Ala Glu Leu Leu Ile Val Thr Thr Pro Gln Ala Ala
245 250 255
Ala Ala Glu Val Ala Glu Arg Ala Gly Thr Ile Ser Val Gln Thr Asn
260 265 270
Gln Lys Val Ala Gly Val Ile Glu Asn Met Ser Ala Met Val Leu Pro
275 280 285
Asp Gly Thr Thr Met Asp Val Phe Gly Thr Gly Gly Gly Gln Lys Ile
290 295 300
Ala Asp Arg Leu Thr Ala Val Thr Gly Glu Glu Val Lys Val Ile Gly
305 310 315 320
Ser Val Pro Leu Asp Pro Asn Leu Arg Ile Gly Gly Asp Val Gly Asn
325 330 335
Pro Ile Ala Ile Ser Glu Pro His Ser Pro Thr Ala Ala Ala Ile Asn
340 345 350
Glu Ile Ala Glu His Leu Ala His Arg Lys Val Ser Leu Val Gly Lys
355 360 365
Thr Leu Gly Leu Gly Val Lys
370 375
<210>237
<211>475
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(445)
<223>RXA02084
<400>237
cgttgatggt tgggtggtgg gtcacagttt agaaaaagtg acttcaaatc acgtcgaaaa 60
gaaattgaaa gtacttaaga aacatgtcac gctaggagac gtg ata atc att gca 115
Val Ile Ile Ile Ala
1 5
agc gtt gtt ttc ctc ctc gtt ggc gca atg ttg gcg aat gct gcc gct 163
Ser Val Val Phe Leu Leu Val Gly Ala Met Leu Ala Asn Ala Ala Ala
10 15 20
gcg ctc ttt agt gcc agc gag cca ttc gga cga att tcc tac ctc att 211
Ala Leu Phe Ser Ala Ser Glu Pro Phe Gly Arg Ile Ser Tyr Leu Ile
25 30 35
ggc cta cct aac gaa gac gac ttc gtg ccg tac tca cta cgg ttc gtg 259
Gly Leu Pro Asn Glu Asp Asp Phe Val Pro Tyr Ser Leu Arg Phe Val
40 45 50
gcc ttt ttc cca ctg atg ctc tcc gca tcg atg gca gca tcc ttc ttc 307
Ala Phe Phe Pro Leu Met Leu Ser Ala Ser Met Ala Ala Ser Phe Phe
55 60 65
ggg gtg tgg gca gta cta atc att cct ttc ggt tac ttc cca tca ctg 355
Gly Val Trp Ala Val Leu Ile Ile Pro Phe Gly Tyr Phe Pro Ser Leu
70 75 80 85
atg atg gtt cat aag cac aac aag cag gta cag cga acc tgg gat tcc 403
Met Met Val His Lys His Asn Lys Gln Val Gln Arg Thr Trp Asp Ser
90 95 100
gtc acc gtt gct gac ttc tac gag gat tcc acc cct ctg gtc 445
Val Thr Val Ala Asp Phe Tyr Glu Asp Ser Thr Pro Leu Val
105 110 115
taatttcgat ttctaaatgt catattggcc 475
<210>238
<211>115
<212>PRT
<213>谷氨酸棒状杆菌
<400>238
Val Ile Ile Ile Ala Ser Val Val Phe Leu Leu Val Gly Ala Met Leu
1 5 10 15
Ala Asn Ala Ala Ala Ala Leu Phe Ser Ala Ser Glu Pro Phe Gly Arg
20 25 30
Ile Ser Tyr Leu Ile Gly Leu Pro Asn Glu Asp Asp Phe Val Pro Tyr
35 40 45
Ser Leu Arg Phe Val Ala Phe Phe Pro Leu Met Leu Ser Ala Ser Met
50 55 60
Ala Ala Ser Phe Phe Gly Val Trp Ala Val Leu Ile Ile Pro Phe Gly
65 70 75 80
Tyr Phe Pro Ser Leu Met Met Val His Lys His Asn Lys Gln Val Gln
85 90 95
Arg Thr Trp Asp Ser Val Thr Val Ala Asp Phe Tyr Glu Asp Ser Thr
100 105 110
Pro Leu Val
115
<210>239
<211>781
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(751)
<223>RXA02091
<400>239
agatctagcc aacgaacttg atttcatcga gccggaagag acagaagaag agcagcaatt 60
gaagtggctt agccttatgc agttttttgg aagtagataa atg agc atc tgg aaa 115
Met Ser Ile Trp Lys
1 5
cgt ctg tta gtg cag tac ccg cgc ttc gcc gac acc ctc aca gcc ggc 163
Arg Leu Leu Val Gln Tyr Pro Arg Phe Ala Asp Thr Leu Thr Ala Gly
10 15 20
caa ccc atc acg ctc gag gaa tta gca acc ccg gaa gtg atc ttg gaa 211
Gln Pro Ile Thr Leu Glu Glu Leu Ala Thr Pro Glu Val Ile Leu Glu
25 30 35
gct gtt gcc aaa ggc caa gaa att ttc ggc att gag cag cca aaa cat 259
Ala Val Ala Lys Gly Gln Glu Ile Phe Gly Ile Glu Gln Pro Lys His
40 45 50
gca gca caa ctc tgg ttt cac tcc ctg tgc acc gca att gtc ggc ccc 307
Ala Ala Gln Leu Trp Phe His Ser Leu Cys Thr Ala Ile Val Gly Pro
55 60 65
gcc gtc acc gcc atg gtg gaa ttc gat gtc atc ccc agc ctc gac ata 355
Ala Val Thr Ala Met Val Glu Phe Asp Val Ile Pro Ser Leu Asp Ile
70 75 80 85
cgt cga ggt cag ctg cat aac atc gac ggt tac tgg ttc ggc ttc agg 403
Arg Arg Gly Gln Leu His Asn Ile Asp Gly Tyr Trp Phe Gly Phe Arg
90 95 100
ccg gag gag atg ctt gtc gac gcc tcc ctc cac ctg tcg ggc acc caa 451
Pro Glu Glu Met Leu Val Asp Ala Ser Leu His Leu Ser Gly Thr Gln
105 110 115
ttc ggc gag agt atc cgc gtg gtg att gat gca tta tgc gct gcc acg 499
Phe Gly Glu Ser Ile Arg Val Val Ile Asp Ala Leu Cys Ala Ala Thr
120 125 130
gat ctg cga ccg gca ccc ctg tgg gcg gtt gcc tca gat gcg ttg gga 547
Asp Leu Arg Pro Ala Pro Leu Trp Ala Val Ala Ser Asp Ala Leu Gly
135 140 145
atc gca gct agc ggc gca ggt gtc gag gcc ttt gaa gaa gaa cat gcc 595
Ile Ala Ala Ser Gly Ala Gly Val Glu Ala Phe Glu Glu Glu His Ala
150 155 160 165
cgc gag gtg gcg gaa gcc ctc att gaa gga atg aat agt gtg aac tca 643
Arg Glu Val Ala Glu Ala Leu Ile Glu Gly Met Asn Ser Val Asn Ser
170 175 180
gtt cca tcg ccg cgg ttt aac gac gac gat tat ttc att cga gct gga 691
Val Pro Ser Pro Arg Phe Asn Asp Asp Asp Tyr Phe Ile Arg Ala Gly
185 190 195
tgc tgc atg att ttc cac tca cca cga gct gat ttt tgc acg tcg tgc 739
Cys Cys Met Ile Phe His Ser Pro Arg Ala Asp Phe Cys Thr Ser Cys
200 205 210
cca cag aag agg tgaaaggcta ggcgcggatt cgttgtggac 781
Pro Gln Lys Arg
215
<210>240
<211>217
<212>PRT
<213>谷氨酸棒状杆菌
<400>240
Met Ser Ile Trp Lys Arg Leu Leu Val Gln Tyr Pro Arg Phe Ala Asp
1 5 10 15
Thr Leu Thr Ala Gly Gln Pro Ile Thr Leu Glu Glu Leu Ala Thr Pro
20 25 30
Glu Val Ile Leu Glu Ala Val Ala Lys Gly Gln Glu Ile Phe Gly Ile
35 40 45
Glu Gln Pro Lys His Ala Ala Gln Leu Trp Phe His Ser Leu Cys Thr
50 55 60
Ala Ile Val Gly Pro Ala Val Thr Ala Met Val Glu Phe Asp Val Ile
65 70 75 80
Pro Ser Leu Asp Ile Arg Arg Gly Gln Leu His Asn Ile Asp Gly Tyr
85 90 95
Trp Phe Gly Phe Arg Pro Glu Glu Met Leu Val Asp Ala Ser Leu His
100 105 110
Leu Ser Gly Thr Gln Phe Gly Glu Ser Ile Arg Val Val Ile Asp Ala
115 120 125
Leu Cys Ala Ala Thr Asp Leu Arg Pro Ala Pro Leu Trp Ala Val Ala
130 135 140
Ser Asp Ala Leu Gly Ile Ala Ala Ser Gly Ala Gly Val Glu Ala Phe
145 150 155 160
Glu Glu Glu His Ala Arg Glu Val Ala Glu Ala Leu Ile Glu Gly Met
165 170 175
Asn Ser Val Asn Ser Val Pro Ser Pro Arg Phe Asn Asp Asp Asp Tyr
180 185 190
Phe Ile Arg Ala Gly Cys Cys Met Ile Phe His Ser Pro Arg Ala Asp
195 200 205
Phe Cys Thr Ser Cys Pro Gln Lys Arg
210 215
<210>241
<211>3502
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3472)
<223>RXA02097
<400>241
ttggctacag atgtttcagc gcttgcagtg gggtagtgtg tttaaacatc acaattagtt 60
ctagaggaaa acgcattttt tgcgcgggga gagtgtatac atg ccg gct ggc atc 115
Met Pro Ala Gly Ile
1 5
gca gac atg aca gat tca ttg ctc gga tgg gca tca caa act gag ctg 163
Ala Asp Met Thr Asp Ser Leu Leu Gly Trp Ala Ser Gln Thr Glu Leu
10 15 20
gat ctg aac cag cgt ctt gca ggg gta gag tac ttt cca caa att cag 211
Asp Leu Asn Gln Arg Leu Ala Gly Val Glu Tyr Phe Pro Gln Ile Gln
25 30 35
ctg cga cac gat gag ctc gag cgc att cat cgg ttt tac ggc acc ttt 259
Leu Arg His Asp Glu Leu Glu Arg Ile His Arg Phe Tyr Gly Thr Phe
40 45 50
ttg tcc cgc cag gta ggc gcg ggc gca agc ctt ggg gat ctt ttt gaa 307
Leu Ser Arg Gln Val Gly Ala Gly Ala Ser Leu Gly Asp Leu Phe Glu
55 60 65
atg acc cca tgc ctg aca gtc acc acc ttg gtg tct cgg gcg tca cgg 355
Met Thr Pro Cys Leu Thr Val Thr Thr Leu Val Ser Arg Ala Ser Arg
70 75 80 85
atc agc gat cca gca gat ttc ttc ggt gaa tac atc gga gga ctg gga 403
Ile Ser Asp Pro Ala Asp Phe Phe Gly Glu Tyr Ile Gly Gly Leu Gly
90 95 100
ctt agc gca gaa cac gca gca gtt gtt gaa ggg ttg acc gaa aag ctc 451
Leu Ser Ala Glu His Ala Ala Val Val Glu Gly Leu Thr Glu Lys Leu
105 110 115
ttc gca cag gct ggc ctg ctc gtt cct gag gga att gca tct cca ttg 499
Phe Ala Gln Ala Gly Leu Leu Val Pro Glu Gly Ile Ala Ser Pro Leu
120 125 130
gag ttg tta tcc atc cac gca ggc att agt aac cac gaa gtg gcc gca 547
Glu Leu Leu Ser Ile His Ala Gly Ile Ser Asn His Glu Val Ala Ala
135 140 145
gtg ctg acc gaa gtg gaa aac ggc acc acc gaa tat cca ttc atg ttc 595
Val Leu Thr Glu Val Glu Asn Gly Thr Thr Glu Tyr Pro Phe Met Phe
150 155 160 165
gac gct gtc ctg cgc cta acc cct gag tgg gca cag acc ctt atc ggc 643
Asp Ala Val Leu Arg Leu Thr Pro Glu Trp Ala Gln Thr Leu Ile Gly
170 175 180
gga gtt caa gaa ctc att gaa ttt gcc acc acc cac cga act tct tgg 691
Gly Val Gln Glu Leu Ile Glu Phe Ala Thr Thr His Arg Thr Ser Trp
185 190 195
tca gac cgc cag cgc gaa tcc tca ctg cca gcc atg atc gat gag atc 739
Ser Asp Arg Gln Arg Glu Ser Ser Leu Pro Ala Met Ile Asp Glu Ile
200 205 210
gtt gtg gcg gaa ctt cgg gaa cgc cca gtt ggt act gcc gac cgt gaa 787
Val Val Ala Glu Leu Arg Glu Arg Pro Val Gly Thr Ala Asp Arg Glu
215 220 225
aac tcc gtt ggt gtg gca ctt cgt gag ctt cgc cca cgc ctc atc ctg 835
Asn Ser Val Gly Val Ala Leu Arg Glu Leu Arg Pro Arg Leu Ile Leu
230 235 240 245
gat gca gaa cgc cgc aaa gtc tgc ctg cgt cta cct gaa cag cgc gtc 883
Asp Ala Glu Arg Arg Lys Val Cys Leu Arg Leu Pro Glu Gln Arg Val
250 255 260
agc gac gat gaa atc aac tgg cga gtc agc cta gaa ggc acc acc cgg 931
Ser Asp Asp Glu Ile Asn Trp Arg Val Ser Leu Glu Gly Thr Thr Arg
265 270 275
att ttc tcc acc cgc cga gca tgg ggc gat act tct gga tac tcc gaa 979
Ile Phe Ser Thr Arg Arg Ala Trp Gly Asp Thr Ser Gly Tyr Ser Glu
280 285 290
gcc ctc gac atc act gtc gag cgt caa atc cgc gaa acc acc gtc acc 1027
Ala Leu Asp Ile Thr Val Glu Arg Gln Ile Arg Glu Thr Thr Val Thr
295 300 305
gac acc tca aac caa atc acc tgg gtt gtc cca gtc gtg gac ttc aac 1075
Asp Thr Ser Asn Gln Ile Thr Trp Val Val Pro Val Val Asp Phe Asn
310 315 320 325
gac cca gtg ctg gtg ttt tcc gcg cgc ggt gaa aac ctc acc gac aag 1123
Asp Pro Val Leu Val Phe Ser Ala Arg Gly Glu Asn Leu Thr Asp Lys
330 335 340
gtc tcc ctg cac cat caa gag att tac gtt ctc gcg cca gcg gaa gca 1171
Val Ser Leu His His Gln Glu Ile Tyr Val Leu Ala Pro Ala Glu Ala
345 350 355
aaa ctc gaa gac atg gtc act ggc cag cca gta cca gtt att gag caa 1219
Lys Leu Glu Asp Met Val Thr Gly Gln Pro Val Pro Val Ile Glu Gln
360 365 370
ttc ctc gta gag ggc tgg aac tca tgg gtg tgc tcc cgc gtg gac gcc 1267
Phe Leu Val Glu Gly Trp Asn Ser Trp Val Cys Ser Arg Val Asp Ala
375 380 385
cgt ggc ctg tcc tct ctg aag gtc aac aaa gaa gtc cga tgc att gac 1315
Arg Gly Leu Ser Ser Leu Lys Val Asn Lys Glu Val Arg Cys Ile Asp
390 395 400 405
cca cgt cga cgc gtt gcc ttc cac cac cca gcc gaa ttg gtc cct cac 1363
Pro Arg Arg Arg Val Ala Phe His His Pro Ala Glu Leu Val Pro His
410 415 420
gta cga tcc att tcc gga ctc ccc gta cac gcg cag tcc ctg atc gcc 1411
Val Arg Ser Ile Ser Gly Leu Pro Val His Ala Gln Ser Leu Ile Ala
425 430 435
gag ttc cca cca acc ctg agc gga caa gac gaa acc tgg atg ctc tcc 1459
Glu Phe Pro Pro Thr Leu Ser Gly Gln Asp Glu Thr Trp Met Leu Ser
440 445 450
atc tcg gct ttc gca ggt gta ggc gct gct ggt gaa gaa atc gcc gag 1507
Ile Ser Ala Phe Ala Gly Val Gly Ala Ala Gly Glu Glu Ile Ala Glu
455 460 465
cca gag cct ttg gaa gtc cct gcc gac ggt ggc ctt ttc gcc atc ttc 1555
Pro Glu Pro Leu Glu Val Pro Ala Asp Gly Gly Leu Phe Ala Ile Phe
470 475 480 485
gac cca gaa ata tac gac gcc cca tgg gtg ggt gaa tac ctg gtc cga 1603
Asp Pro Glu Ile Tyr Asp Ala Pro Trp Val Gly Glu Tyr Leu Val Arg
490 495 500
ctc cgc ggc cca cgc aat gaa tcc ttc cga ccc gaa ttc gcc atc gtc 1651
Leu Arg Gly Pro Arg Asn Glu Ser Phe Arg Pro Glu Phe Ala Ile Val
505 510 515
gaa gac atg acc acc gaa ttc gaa gtc gcc tca ggt gca tca ttt cga 1699
Glu Asp Met Thr Thr Glu Phe Glu Val Ala Ser Gly Ala Ser Phe Arg
520 525 530
atc cca acc acc act ggt ctc agc gaa gcc agc cta cgc gtg cgt tcc 1747
Ile Pro Thr Thr Thr Gly Leu Ser Glu Ala Ser Leu Arg Val Arg Ser
535 540 545
ggt gaa aag cac ttc acc gca gag cca cgc ctg gtc acc gtt gaa gca 1795
Gly Glu Lys His Phe Thr Ala Glu Pro Arg Leu Val Thr Val Glu Ala
550 555 560 565
acc gac ccc aac gca tca ttc gtg gtc acc acc gat gaa ggc gat caa 1843
Thr Asp Pro Asn Ala Ser Phe Val Val Thr Thr Asp Glu Gly Asp Gln
570 575 580
atg cca ttg cga ttt gtg cca cca caa atc gcc atc gaa ctt cca ctg 1891
Met Pro Leu Arg Phe Val Pro Pro Gln Ile Ala Ile Glu Leu Pro Leu
585 590 595
acc acc gag cca cca acc tgg cgc gtc acc cgt act gtc tgt gga cca 1939
Thr Thr Glu Pro Pro Thr Trp Arg Val Thr Arg Thr Val Cys Gly Pro
600 605 610
cgc gac ctc gac ggt gca ggc gaa ctc cgc atc cgc acc ggt gtc gat 1987
Arg Asp Leu Asp Gly Ala Gly Glu Leu Arg Ile Arg Thr Gly Val Asp
615 620 625
gtc ggc gat cca aag gtc agt gtg cgc aac cac cac ggt tca cca ctg 2035
Val Gly Asp Pro Lys Val Ser Val Arg Asn His His Gly Ser Pro Leu
630 635 640 645
cga acc gtg aaa atg gtc acc cct gac aac ggc cgt acc tgg att gcc 2083
Arg Thr Val Lys Met Val Thr Pro Asp Asn Gly Arg Thr Trp Ile Ala
650 655 660
agc atg aag gaa atc gca gcc agt acc ttt gtg atg cca cgc gga tcc 2131
Ser Met Lys Glu Ile Ala Ala Ser Thr Phe Val Met Pro Arg Gly Ser
665 670 675
atc gaa ttt gag tgg act gac cgc aag gtt gac cgt cgc gtt tcc gtg 2179
Ile Glu Phe Glu Trp Thr Asp Arg Lys Val Asp Arg Arg Val Ser Val
680 685 690
acg att gct gtc att gac aaa act gag aac ttt act ggc atc acc atc 2227
Thr Ile Ala Val Ile Asp Lys Thr Glu Asn Phe Thr Gly Ile Thr Ile
695 700 705
gaa gat gga aag ctc gta ttc gaa gaa ctc gca gcc ggt cgc caa ctc 2275
Glu Asp Gly Lys Leu Val Phe Glu Glu Leu Ala Ala Gly Arg Gln Leu
710 715 720 725
gct gca tgg gtg tgg cca caa acc gca ccg tgg gta agc gca gtg gaa 2323
Ala Ala Trp Val Trp Pro Gln Thr Ala Pro Trp Val Ser Ala Val Glu
730 735 740
ctt gct gtc acc gga cca gag ctg gaa ctc cct gaa gtt ctc gtc ggc 2371
Leu Ala Val Thr Gly Pro Glu Leu Glu Leu Pro Glu Val Leu Val Gly
745 750 755
gca ggc aac ctg att gtt caa ctc cac acc gct gac cca ttc act acc 2419
Ala Gly Asn Leu Ile Val Gln Leu His Thr Ala Asp Pro Phe Thr Thr
760 765 770
tcc gtg acc cca ctg tca cca gga aaa gct gcg gtc acc gtt gag caa 2467
Ser Val Thr Pro Leu Ser Pro Gly Lys Ala Ala Val Thr Val Glu Gln
775 780 785
gaa ggc tac tac tca gca caa acc gaa gaa tat gca cag ctt tca gca 2515
Glu Gly Tyr Tyr Ser Ala Gln Thr Glu Glu Tyr Ala Gln Leu Ser Ala
790 795 800 805
ttc ttc ggt ggg gaa gta gaa gaa cca cca atc agt gac gct gtg gtc 2563
Phe Phe Gly Gly Glu Val Glu Glu Pro Pro Ile Ser Asp Ala Val Val
810 815 820
ccc gca ctt tgg gat gtt tcc cat atc tgg acc gaa cag gga aac acc 2611
Pro Ala Leu Trp Asp Val Ser His Ile Trp Thr Glu Gln Gly Asn Thr
825 830 835
gag cat ctt cca gta gtc cat gcc gcc ctg cgc tcc tca cca gcc gca 2659
Glu His Leu Pro Val Val His Ala Ala Leu Arg Ser Ser Pro Ala Ala
840 845 850
gca ctg aag ggt ctg tcc gct tcg ctg gtt ccc gca cag gca cta cct 2707
Ala Leu Lys Gly Leu Ser Ala Ser Leu Val Pro Ala Gln Ala Leu Pro
855 860 865
gga aaa gtc att tcc tcc gga ctg gca gcc tca ccg ttc acc acg gaa 2755
Gly Lys Val Ile Ser Ser Gly Leu Ala Ala Ser Pro Phe Thr Thr Glu
870 875 880 885
tca cca gca aca gaa gtg cac cgc acc gca tgg atc gga acc ctg caa 2803
Ser Pro Ala Thr Glu Val His Arg Thr Ala Trp Ile Gly Thr Leu Gln
890 895 900
ctc ctg ggt gca ctg cca agc gca ttc aag gaa gcc gaa gag ctt ggc 2851
Leu Leu Gly Ala Leu Pro Ser Ala Phe Lys Glu Ala Glu Glu Leu Gly
905 910 915
aac cgc aca cca ctg ctg cca atc ctc gga caa ctt gag gaa gtc gcc 2899
Asn Arg Thr Pro Leu Leu Pro Ile Leu Gly Gln Leu Glu Glu Val Ala
920 925 930
ggc aag aac atc ctg tcc acc ctt gca act ggc cgt gac tcc act ttg 2947
Gly Lys Asn Ile Leu Ser Thr Leu Ala Thr Gly Arg Asp Ser Thr Leu
935 940 945
gac acc gca tgc atc gac caa tcc acc gtt gcg att gcc ggc atg aac 2995
Asp Thr Ala Cys Ile Asp Gln Ser Thr Val Ala Ile Ala Gly Met Asn
950 955 960 965
gaa acc cag caa aaa gcc ctg ctg gac atg ttc ttc agc aac gcc gac 3043
Glu Thr Gln Gln Lys Ala Leu Leu Asp Met Phe Phe Ser Asn Ala Asp
970 975 980
atc gtt cct gga cca cta atg gaa gac aac acc cgc ctc atg gca gtg 3091
Ile Val Pro Gly Pro Leu Met Glu Asp Asn Thr Arg Leu Met Ala Val
985 990 995
ttc gaa acc ttc aag aag cgc gat gca ctc cgt gag gtt ctc cag act 3139
Phe Glu Thr Phe Lys Lys Arg Asp Ala Leu Arg Glu Val Leu Gln Thr
1000 1005 1010
gaa ggc ttg att aag acc gct gta gaa ctt ctt cgt gcc atg cgt gga 3187
Glu Gly Leu Ile Lys Thr Ala Val Glu Leu Leu Arg Ala Met Arg Gly
1015 1020 1025
acc cag cgt cag ctg tat tcc tcc gca cgt att cga ttc gac aag ctc 3235
Thr Gln Arg Gln Leu Tyr Ser Ser Ala Arg Ile Arg Phe Asp Lys Leu
1030 1035 1040 1045
gat ggt gtc aac act gac aac cca gaa aac atg tgg gca ctc acc cca 3283
Asp Gly Val Asn Thr Asp Asn Pro Glu Asn Met Trp Ala Leu Thr Pro
1050 1055 1060
gtt gtg tca ctg gtg ttc gcg ttg tca tcc cgt ttg cat gca cac gaa 3331
Val Val Ser Leu Val Phe Ala Leu Ser Ser Arg Leu His Ala His Glu
1065 1070 1075
ttg atc ggc aag acc cga act ctc gat cgt gca tct gcc ggt tgg ggt 3379
Leu Ile Gly Lys Thr Arg Thr Leu Asp Arg Ala Ser Ala Gly Trp Gly
1080 1085 1090
cga atc gct gat ctg gtg cca gac ctt gtc acc ggt gac ttg atc tcc 3427
Arg Ile Ala Asp Leu Val Pro Asp Leu Val Thr Gly Asp Leu Ile Ser
1095 1100 1105
gcg gag gca atg gtt ttg gga gct cga aac cca gga ctc gtc gat 3472
Ala Glu Ala Met Val Leu Gly Ala Arg Asn Pro Gly Leu Val Asp
1110 1115 1120
tagtccctga tttcatcgga gggcacctag 3502
<210>242
<211>1124
<212>PRT
<213>谷氨酸棒状杆菌
<400>242
Met Pro Ala Gly Ile Ala Asp Met Thr Asp Ser Leu Leu Gly Trp Ala
1 5 10 15
Ser Gln Thr Glu Leu Asp Leu Asn Gln Arg Leu Ala Gly Val Glu Tyr
20 25 30
Phe Pro Gln Ile Gln Leu Arg His Asp Glu Leu Glu Arg Ile His Arg
35 40 45
Phe Tyr Gly Thr Phe Leu Ser Arg Gln Val Gly Ala Gly Ala Ser Leu
50 55 60
Gly Asp Leu Phe Glu Met Thr Pro Cys Leu Thr Val Thr Thr Leu Val
65 70 75 80
Ser Arg Ala Ser Arg Ile Ser Asp Pro Ala Asp Phe Phe Gly Glu Tyr
85 90 95
Ile Gly Gly Leu Gly Leu Ser Ala Glu His Ala Ala Val Val Glu Gly
100 105 110
Leu Thr Glu Lys Leu Phe Ala Gln Ala Gly Leu Leu Val Pro Glu Gly
115 120 125
Ile Ala Ser Pro Leu Glu Leu Leu Ser Ile His Ala Gly Ile Ser Asn
130 135 140
His Glu Val Ala Ala Val Leu Thr Glu Val Glu Asn Gly Thr Thr Glu
145 150 155 160
Tyr Pro Phe Met Phe Asp Ala Val Leu Arg Leu Thr Pro Glu Trp Ala
165 170 175
Gln Thr Leu Ile Gly Gly Val Gln Glu Leu Ile Glu Phe Ala Thr Thr
180 185 190
His Arg Thr Ser Trp Ser Asp Arg Gln Arg Glu Ser Ser Leu Pro Ala
195 200 205
Met Ile Asp Glu Ile Val Val Ala Glu Leu Arg Glu Arg Pro Val Gly
210 215 220
Thr Ala Asp Arg Glu Asn Ser Val Gly Val Ala Leu Arg Glu Leu Arg
225 230 235 240
Pro Arg Leu Ile Leu Asp Ala Glu Arg Arg Lys Val Cys Leu Arg Leu
245 250 255
Pro Glu Gln Arg Val Ser Asp Asp Glu Ile Asn Trp Arg Val Ser Leu
260 265 270
Glu Gly Thr Thr Arg Ile Phe Ser Thr Arg Arg Ala Trp Gly Asp Thr
275 280 285
Ser Gly Tyr Ser Glu Ala Leu Asp Ile Thr Val Glu Arg Gln Ile Arg
290 295 300
Glu Thr Thr Val Thr Asp Thr Ser Asn Gln Ile Thr Trp Val Val Pro
305 310 315 320
Val Val Asp Phe Asn Asp Pro Val Leu Val Phe Ser Ala Arg Gly Glu
325 330 335
Asn Leu Thr Asp Lys Val Ser Leu His His Gln Glu Ile Tyr Val Leu
340 345 350
Ala Pro Ala Glu Ala Lys Leu Glu Asp Met Val Thr Gly Gln Pro Val
355 360 365
Pro Val Ile Glu Gln Phe Leu Val Glu Gly Trp Asn Ser Trp Val Cys
370 375 380
Ser Arg Val Asp Ala Arg Gly Leu Ser Ser Leu Lys Val Asn Lys Glu
385 390 395 400
Val Arg Cys Ile Asp Pro Arg Arg Arg Val Ala Phe His His Pro Ala
405 410 415
Glu Leu Val Pro His Val Arg Ser Ile Ser Gly Leu Pro Val His Ala
420 425 430
Gln Ser Leu Ile Ala Glu Phe Pro Pro Thr Leu Ser Gly Gln Asp Glu
435 440 445
Thr Trp Met Leu Ser Ile Ser Ala Phe Ala Gly Val Gly Ala Ala Gly
450 455 460
Glu Glu Ile Ala Glu Pro Glu Pro Leu Glu Val Pro Ala Asp Gly Gly
465 470 475 480
Leu Phe Ala Ile Phe Asp Pro Glu Ile Tyr Asp Ala Pro Trp Val Gly
485 490 495
Glu Tyr Leu Val Arg Leu Arg Gly Pro Arg Asn Glu Ser Phe Arg Pro
500 505 510
Glu Phe Ala Ile Val Glu Asp Met Thr Thr Glu Phe Glu Val Ala Ser
515 520 525
Gly Ala Ser Phe Arg Ile Pro Thr Thr Thr Gly Leu Ser Glu Ala Ser
530 535 540
Leu Arg Val Arg Ser Gly Glu Lys His Phe Thr Ala Glu Pro Arg Leu
545 550 555 560
Val Thr Val Glu Ala Thr Asp Pro Asn Ala Ser Phe Val Val Thr Thr
565 570 575
Asp Glu Gly Asp Gln Met Pro Leu Arg Phe Val Pro Pro Gln Ile Ala
580 585 590
Ile Glu Leu Pro Leu Thr Thr Glu Pro Pro Thr Trp Arg Val Thr Arg
595 600 605
Thr Val Cys Gly Pro Arg Asp Leu Asp Gly Ala Gly Glu Leu Arg Ile
610 615 620
Arg Thr Gly Val Asp Val Gly Asp Pro Lys Val Ser Val Arg Asn His
625 630 635 640
His Gly Ser Pro Leu Arg Thr Val Lys Met Val Thr Pro Asp Asn Gly
645 650 655
Arg Thr Trp Ile Ala Ser Met Lys Glu Ile Ala Ala Ser Thr Phe Val
660 665 670
Met Pro Arg Gly Ser Ile Glu Phe Glu Trp Thr Asp Arg Lys Val Asp
675 680 685
Arg Arg Val Ser Val Thr Ile Ala Val Ile Asp Lys Thr Glu Asn Phe
690 695 700
Thr Gly Ile Thr Ile Glu Asp Gly Lys Leu Val Phe Glu Glu Leu Ala
705 710 715 720
Ala Gly Arg Gln Leu Ala Ala Trp Val Trp Pro Gln Thr Ala Pro Trp
725 730 735
Val Ser Ala Val Glu Leu Ala Val Thr Gly Pro Glu Leu Glu Leu Pro
740 745 750
Glu Val Leu Val Gly Ala Gly Asn Leu Ile Val Gln Leu His Thr Ala
755 760 765
Asp Pro Phe Thr Thr Ser Val Thr Pro Leu Ser Pro Gly Lys Ala Ala
770 775 780
Val Thr Val Glu Gln Glu Gly Tyr Tyr Ser Ala Gln Thr Glu Glu Tyr
785 790 795 800
Ala Gln Leu Ser Ala Phe Phe Gly Gly Glu Val Glu Glu Pro Pro Ile
805 810 815
Ser Asp Ala Val Val Pro Ala Leu Trp Asp Val Ser His Ile Trp Thr
820 825 830
Glu Gln Gly Asn Thr Glu His Leu Pro Val Val His Ala Ala Leu Arg
835 840 845
Ser Ser Pro Ala Ala Ala Leu Lys Gly Leu Ser Ala Ser Leu Val Pro
850 855 860
Ala Gln Ala Leu Pro Gly Lys Val Ile Ser Ser Gly Leu Ala Ala Ser
865 870 875 880
Pro Phe Thr Thr Glu Ser Pro Ala Thr Glu Val His Arg Thr Ala Trp
885 890 895
Ile Gly Thr Leu Gln Leu Leu Gly Ala Leu Pro Ser Ala Phe Lys Glu
900 905 910
Ala Glu Glu Leu Gly Asn Arg Thr Pro Leu Leu Pro Ile Leu Gly Gln
915 920 925
Leu Glu Glu Val Ala Gly Lys Asn Ile Leu Ser Thr Leu Ala Thr Gly
930 935 940
Arg Asp Ser Thr Leu Asp Thr Ala Cys Ile Asp Gln Ser Thr Val Ala
945 950 955 960
Ile Ala Gly Met Asn Glu Thr Gln Gln Lys Ala Leu Leu Asp Met Phe
965 970 975
Phe Ser Asn Ala Asp Ile Val Pro Gly Pro Leu Met Glu Asp Asn Thr
980 985 990
Arg Leu Met Ala Val Phe Glu Thr Phe Lys Lys Arg Asp Ala Leu Arg
995 1000 1005
Glu Val Leu Gln Thr Glu Gly Leu Ile Lys Thr Ala Val Glu Leu Leu
1010 1015 1020
Arg Ala Met Arg Gly Thr Gln Arg Gln Leu Tyr Ser Ser Ala Arg Ile
1025 1030 1035 1040
Arg Phe Asp Lys Leu Asp Gly Val Asn Thr Asp Asn Pro Glu Asn Met
1045 1050 1055
Trp Ala Leu Thr Pro Val Val Ser Leu Val Phe Ala Leu Ser Ser Arg
1060 1065 1070
Leu His Ala His Glu Leu Ile Gly Lys Thr Arg Thr Leu Asp Arg Ala
1075 1080 1085
Ser Ala Gly Trp Gly Arg Ile Ala Asp Leu Val Pro Asp Leu Val Thr
1090 1095 1100
Gly Asp Leu Ile Ser Ala Glu Ala Met Val Leu Gly Ala Arg Asn Pro
1105 1110 1115 1120
Gly Leu Val Asp
<210>243
<211>1351
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1321)
<223>RXA02104
<400>243
accttttcga tagccgtata tgccatggac ccactcctca attgggcgac cgttagactt 60
gaaacactaa ggctagagat tttaactaaa gagtctcctc atg gat tcc cat ttg 115
Met Asp Ser His Leu
1 5
gag cct tat tta cac agg cca cct ggc ttg ttt ccc acc gcg atg tgc 163
Glu Pro Tyr Leu His Arg Pro Pro Gly Leu Phe Pro Thr Ala Met Cys
10 15 20
cac aat aac gcc ata aca gaa agg cat act gac aca atg tcc aac tcc 211
His Asn Asn Ala Ile Thr Glu Arg His Thr Asp Thr Met Ser Asn Ser
25 30 35
ccc acc gac gtt tca acg agc gca cct cga att atg atc gcg cca gat 259
Pro Thr Asp Val Ser Thr Ser Ala Pro Arg Ile Met Ile Ala Pro Asp
40 45 50
tcc tac aaa gga acc gct acc gca tca gaa gct gcg caa tac tta ggc 307
Ser Tyr Lys Gly Thr Ala Thr Ala Ser Glu Ala Ala Gln Tyr Leu Gly
55 60 65
gaa ggc gtg ttg gaa att ttg ccc aac gcc tcc att acg ttg gca ccc 355
Glu Gly Val Leu Glu Ile Leu Pro Asn Ala Ser Ile Thr Leu Ala Pro
70 75 80 85
atg gcc gac ggt ggc gaa gga aca tcc tca gtt ttc ggc ggg cag gtc 403
Met Ala Asp Gly Gly Glu Gly Thr Ser Ser Val Phe Gly Gly Gln Val
90 95 100
ata aca tta ccc acg aca aat gcc gcg gga cgc ctc acc gaa gcc agc 451
Ile Thr Leu Pro Thr Thr Asn Ala Ala Gly Arg Leu Thr Glu Ala Ser
105 110 115
tac acc tta gat tcc gaa aca aac act gcc tac att gac atc gcc gca 499
Tyr Thr Leu Asp Ser Glu Thr Asn Thr Ala Tyr Ile Asp Ile Ala Ala
120 125 130
gcc tcc ggt ttg ccc gcc gtt gca gac gat cta gtc ccc acc acc ggc 547
Ala Ser Gly Leu Pro Ala Val Ala Asp Asp Leu Val Pro Thr Thr Gly
135 140 145
gat acc tac ggc acc ggc gtt ttg atc gca gac gcg gtc acc cgt ggc 595
Asp Thr Tyr Gly Thr Gly Val Leu Ile Ala Asp Ala Val Thr Arg Gly
150 155 160 165
gca act cgc att gct tta ggc ctt ggc gga tca gcc aca act gac gcc 643
Ala Thr Arg Ile Ala Leu Gly Leu Gly Gly Ser Ala Thr Thr Asp Ala
170 175 180
ggc tca gga att ctc atc gcc ctc ggc gcc gtc cca cgc aac aaa gag 691
Gly Ser Gly Ile Leu Ile Ala Leu Gly Ala Val Pro Arg Asn Lys Glu
185 190 195
ggc tac gca cta cga acc ggt ggc gcc gac ctc atc aac ctc gat tac 739
Gly Tyr Ala Leu Arg Thr Gly Gly Ala Asp Leu Ile Asn Leu Asp Tyr
200 205 210
atc gat acc gcc gaa ctc aac atc ccc gca gcc gcc gtc gaa tgg atc 787
Ile Asp Thr Ala Glu Leu Asn Ile Pro Ala Ala Ala Val Glu Trp Ile
215 220 225
ctg ctc acc gat gtc gac gcc ccc gcc acc ggc ccg cag ggc gcg gcc 835
Leu Leu Thr Asp Val Asp Ala Pro Ala Thr Gly Pro Gln Gly Ala Ala
230 235 240 245
acc gta ttc ggg ccc caa aaa ggt gcc acc gaa aaa gac att tcg ctt 883
Thr Val Phe Gly Pro Gln Lys Gly Ala Thr Glu Lys Asp Ile Ser Leu
250 255 260
ctc gac gcc gcc ctc cac cac gcc tgc gcc caa ctg gaa gtt gat ggc 931
Leu Asp Ala Ala Leu His His Ala Cys Ala Gln Leu Glu Val Asp Gly
265 270 275
aca aag cca ggt atg ggc gca gcc ggg ggc att gca atc gga ctg acg 979
Thr Lys Pro Gly Met Gly Ala Ala Gly Gly Ile Ala Ile Gly Leu Thr
280 285 290
tgg ctg tcc acc ctc atg cac ggc aac gac caa cag atc cat atc ctc 1027
Trp Leu Ser Thr Leu Met His Gly Asn Asp Gln Gln Ile His Ile Leu
295 300 305
ccc ggc gcg cca ctg att gcc cgc tcc aac gga atc gag gat gcg ctg 1075
Pro Gly Ala Pro Leu Ile Ala Arg Ser Asn Gly Ile Glu Asp Ala Leu
310 315 320 325
cca gaa act gac ttg ttg atc acc ggt gaa ggc cga cta gat tcc caa 1123
Pro Glu Thr Asp Leu Leu Ile Thr Gly Glu Gly Arg Leu Asp Ser Gln
330 335 340
tcg ttc acc gga aag gtt gtg ggc acc ctc cac ggt tta gct aaa gcc 1171
Ser Phe Thr Gly Lys Val Val Gly Thr Leu His Gly Leu Ala Lys Ala
345 350 355
cac gat gtg gat ctc gct gtt gcg gcc ggc atc gtg gaa ggc ggt att 1219
His Asp Val Asp Leu Ala Val Ala Ala Gly Ile Val Glu Gly Gly Ile
360 365 370
ccc gat gat ttc cta gcg gta gaa atg att aaa tcc tcc gac gtt gca 1267
Pro Asp Asp Phe Leu Ala Val Glu Met Ile Lys Ser Ser Asp Val Ala
375 380 385
gca caa tta cgt gat gca ggc cga agg atc gct caa gaa tac gta gct 1315
Ala Gln Leu Arg Asp Ala Gly Arg Arg Ile Ala Gln Glu Tyr Val Ala
390 395 400 405
caa aac tagcgaagga tctccacagt ccatgggtag 1351
Gln Asn
<210>244
<211>407
<212>PRT
<213>谷氨酸棒状杆菌
<400>244
Met Asp Ser His Leu Glu Pro Tyr Leu His Arg Pro Pro Gly Leu Phe
1 5 10 15
Pro Thr Ala Met Cys His Asn Asn Ala Ile Thr Glu Arg His Thr Asp
20 25 30
Thr Met Ser Asn Ser Pro Thr Asp Val Ser Thr Ser Ala Pro Arg Ile
35 40 45
Met Ile Ala Pro Asp Ser Tyr Lys Gly Thr Ala Thr Ala Ser Glu Ala
50 55 60
Ala Gln Tyr Leu Gly Glu Gly Val Leu Glu Ile Leu Pro Asn Ala Ser
65 70 75 80
Ile Thr Leu Ala Pro Met Ala Asp Gly Gly Glu Gly Thr Ser Ser Val
85 90 95
Phe Gly Gly Gln Val Ile Thr Leu Pro Thr Thr Asn Ala Ala Gly Arg
100 105 110
Leu Thr Glu Ala Ser Tyr Thr Leu Asp Ser Glu Thr Asn Thr Ala Tyr
115 120 125
Ile Asp Ile Ala Ala Ala Ser Gly Leu Pro Ala Val Ala Asp Asp Leu
130 135 140
Val Pro Thr Thr Gly Asp Thr Tyr Gly Thr Gly Val Leu Ile Ala Asp
145 150 155 160
Ala Val Thr Arg Gly Ala Thr Arg Ile Ala Leu Gly Leu Gly Gly Ser
165 170 175
Ala Thr Thr Asp Ala Gly Ser Gly Ile Leu Ile Ala Leu Gly Ala Val
180 185 190
Pro Arg Asn Lys Glu Gly Tyr Ala Leu Arg Thr Gly Gly Ala Asp Leu
195 200 205
Ile Asn Leu Asp Tyr Ile Asp Thr Ala Glu Leu Asn Ile Pro Ala Ala
210 215 220
Ala Val Glu Trp Ile Leu Leu Thr Asp Val Asp Ala Pro Ala Thr Gly
225 230 235 240
Pro Gln Gly Ala Ala Thr Val Phe Gly Pro Gln Lys Gly Ala Thr Glu
245 250 255
Lys Asp Ile Ser Leu Leu Asp Ala Ala Leu His His Ala Cys Ala Gln
260 265 270
Leu Glu Val Asp Gly Thr Lys Pro Gly Met Gly Ala Ala Gly Gly Ile
275 280 285
Ala Ile Gly Leu Thr Trp Leu Ser Thr Leu Met His Gly Asn Asp Gln
290 295 300
Gln Ile His Ile Leu Pro Gly Ala Pro Leu Ile Ala Arg Ser Asn Gly
305 310 315 320
Ile Glu Asp Ala Leu Pro Glu Thr Asp Leu Leu Ile Thr Gly Glu Gly
325 330 335
Arg Leu Asp Ser Gln Ser Phe Thr Gly Lys Val Val Gly Thr Leu His
340 345 350
Gly Leu Ala Lys Ala His Asp Val Asp Leu Ala Val Ala Ala Gly Ile
355 360 365
Val Glu Gly Gly Ile Pro Asp Asp Phe Leu Ala Val Glu Met Ile Lys
370 375 380
Ser Ser Asp Val Ala Ala Gln Leu Arg Asp Ala Gly Arg Arg Ile Ala
385 390 395 400
Gln Glu Tyr Val Ala Gln Asn
405
<210>245
<211>1561
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1531)
<223>RXA02123
<400>245
accaggcaga acctgaccag ttgttggggt ttgcccatgc cgaatgagta aaaccacagt 60
ggattgagtc ttttcggcag ctgcgtgtga acccggcttc atg aca tgc agc ata 115
Met Thr Cys Ser Ile
1 5
cct gtg cga aaa att cct gac cgc cca ctt aaa aaa agg tct agg gtg 163
Pro Val Arg Lys Ile Pro Asp Arg Pro Leu Lys Lys Arg Ser Arg Val
10 15 20
tgg ggt gtg agt ctt cca cca aat gta caa act gtc ggt gag ctg aag 211
Trp Gly Val Ser Leu Pro Pro Asn Val Gln Thr Val Gly Glu Leu Lys
25 30 35
gcc gcc ggc cac atc tac cga cct ttg cgc gta gaa atc cgc gac aac 259
Ala Ala Gly His Ile Tyr Arg Pro Leu Arg Val Glu Ile Arg Asp Asn
40 45 50
ctc cta gcc aaa ctt cgc agc ggc gaa gat ccg tgg cca ggc ctg cac 307
Leu Leu Ala Lys Leu Arg Ser Gly Glu Asp Pro Trp Pro Gly Leu His
55 60 65
ggc ctg aac tac acg gtc atc ccg cag ctt gaa cgc gcg ctc atc gcc 355
Gly Leu Asn Tyr Thr Val Ile Pro Gln Leu Glu Arg Ala Leu Ile Ala
70 75 80 85
ggc cac gac atc gtg ctg ctc ggc gaa egc ggc cag ggt aaa acc cgc 403
Gly His Asp Ile Val Leu Leu Gly Glu Arg Gly Gln Gly Lys Thr Arg
90 95 100
ctg ctc cgc acg tta ata acg ctt ctc gac gag tgg tcc ccg atc att 451
Leu Leu Arg Thr Leu Ile Thr Leu Leu Asp Glu Trp Ser Pro Ile Ile
105 110 115
ccc ggg cgc gac gtt cca gaa cac cct ctt gcg cca aac gta gag atc 499
Pro Gly Arg Asp Val Pro Glu His Pro Leu Ala Pro Asn Val Glu Ile
120 125 130
tcc gat gat cag ccc att gaa tgg gta cac cgc gat gcg cgc tat acc 547
Ser Asp Asp Gln Pro Ile Glu Trp Val His Arg Asp Ala Arg Tyr Thr
135 140 145
gag aaa ctt gcc acc cca gat act tcc gtg gct gat ctt atc ggt gat 595
Glu Lys Leu Ala Thr Pro Asp Thr Ser Val Ala Asp Leu Ile Gly Asp
150 155 160 165
gtt gac cca atg cgt gtt gct gaa ggc cgc agc ctc ggt gat cta gaa 643
Val Asp Pro Met Arg Val Ala Glu Gly Arg Ser Leu Gly Asp Leu Glu
170 175 180
acc att cac tac ggt ctg att cct cgc gcc aac cgt ggc atc gtg gcg 691
Thr Ile His Tyr Gly Leu Ile Pro Arg Ala Asn Arg Gly Ile Val Ala
185 190 195
atc aac gag ctt cct gac ctc gct gaa cgc atc cag gtt gcc atg ctc 739
Ile Asn Glu Leu Pro Asp Leu Ala Glu Arg Ile Gln Val Ala Met Leu
200 205 210
aac gtc atg gag gaa cgt gat gtg cag att cgt ggc tac aac att cgt 787
Asn Val Met Glu Glu Arg Asp Val Gln Ile Arg Gly Tyr Asn Ile Arg
215 220 225
ttg gac ttg gat gtg ctg gtt gtt gcc tca gcc aac cct gag gat tac 835
Leu Asp Leu Asp Val Leu Val Val Ala Ser Ala Asn Pro Glu Asp Tyr
230 235 240 245
acc aac cgt ggc cga att atc act ccg ctc aaa gac cgc ttc ggt gca 883
Thr Asn Arg Gly Arg Ile Ile Thr Pro Leu Lys Asp Arg Phe Gly Ala
250 255 260
gaa atc cgc acc cac tac cca ctt gag ttg gac gat gaa gtg gca att 931
Glu Ile Arg Thr His Tyr Pro Leu Glu Leu Asp Asp Glu Val Ala Ile
265 270 275
atc cgc cag gaa gca gag ctt gtt gcg caa gtc cct gat atc ttg gtt 979
Ile Arg Gln Glu Ala Glu Leu Val Ala Gln Val Pro Asp Ile Leu Val
280 285 290
gaa att ctt gcc cgc tac acc cga gcg ctt cgt gaa tcc tca tcg gtg 1027
Glu Ile Leu Ala Arg Tyr Thr Arg Ala Leu Arg Glu Ser Ser Ser Val
295 300 305
aat cag cgt tct ggt gtg tct gct cgt ttc tcc att gca ggt gca gaa 1075
Asn Gln Arg Ser Gly Val Ser Ala Arg Phe Ser Ile Ala Gly Ala Glu
310 315 320 325
act gtg gcc gca gca gct ctt cga cgc gca gca gtg ttc ggc gaa gat 1123
Thr Val Ala Ala Ala Ala Leu Arg Arg Ala Ala Val Phe Gly Glu Asp
330 335 340
gag gcc gtt gcc cgc ctg gtt gat ttg gaa gca gcc gtg gaa gtc ctc 1171
Glu Ala Val Ala Arg Leu Val Asp Leu Glu Ala Ala Val Glu Val Leu
345 350 355
ggc ggc aag att gaa ttt gaa tcc ggc gaa gaa gga cgc gaa tgg gaa 1219
Gly Gly Lys Ile Glu Phe Glu Ser Gly Glu Glu Gly Arg Glu Trp Glu
360 365 370
atc ctt gat tat ctc ctg cgc acc gca acc gca gaa gcg ctg cgt tcc 1267
Ile Leu Asp Tyr Leu Leu Arg Thr Ala Thr Ala Glu Ala Leu Arg Ser
375 380 385
act ctg cgc tcg ctg gat ctc acc ccg ctc atc gca gca tta gac ggc 1315
Thr Leu Arg Ser Leu Asp Leu Thr Pro Leu Ile Ala Ala Leu Asp Gly
390 395 400 405
agc atc acc gtt tcc act ggc acg aac atc acc gcg tca gaa ttt ttg 1363
Ser Ile Thr Val Ser Thr Gly Thr Asn Ile Thr Ala Ser Glu Phe Leu
410 415 420
gct tcg ctc ccc gaa ctc ggc gaa agc acg cta tat gac gac atc gct 1411
Ala Ser Leu Pro Glu Leu Gly Glu Ser Thr Leu Tyr Asp Asp Ile Ala
425 430 435
caa gct ttt ggt gcc acc aca cca agt act cgc gcg atg gcc att gag 1459
Gln Ala Phe Gly Ala Thr Thr Pro Ser Thr Arg Ala Met Ala Ile Glu
440 445 450
ctt gct ttg gaa ggt ctt tac ctt tcc cga aaa att gcc aag gat tct 1507
Leu Ala Leu Glu Gly Leu Tyr Leu Ser Arg Lys Ile Ala Lys Asp Ser
455 460 465
ggc gaa ggt gaa acc att tac ggt taactggttt taaggagcac cacagccatg 1561
Gly Glu Gly Glu Thr Ile Tyr Gly
470 475
<210>246
<211>477
<212>PRT
<213>谷氨酸棒状杆菌
<400>246
Met Thr Cys Ser Ile Pro Val Arg Lys Ile Pro Asp Arg Pro Leu Lys
1 5 10 15
Lys Arg Ser Arg Val Trp Gly Val Ser Leu Pro Pro Asn Val Gln Thr
20 25 30
Val Gly Glu Leu Lys Ala Ala Gly His Ile Tyr Arg Pro Leu Arg Val
35 40 45
Glu Ile Arg Asp Asn Leu Leu Ala Lys Leu Arg Ser Gly Glu Asp Pro
50 55 60
Trp Pro Gly Leu His Gly Leu Asn Tyr Thr Val Ile Pro Gln Leu Glu
65 70 75 80
Arg Ala Leu Ile Ala Gly His Asp Ile Val Leu Leu Gly Glu Arg Gly
85 90 95
Gln Gly Lys Thr Arg Leu Leu Arg Thr Leu Ile Thr Leu Leu Asp Glu
100 105 110
Trp Ser Pro Ile Ile Pro Gly Arg Asp Val Pro Glu His Pro Leu Ala
115 120 125
Pro Asn Val Glu Ile Ser Asp Asp Gln Pro Ile Glu Trp Val His Arg
130 135 140
Asp Ala Arg Tyr Thr Glu Lys Leu Ala Thr Pro Asp Thr Ser Val Ala
145 150 155 160
Asp Leu Ile Gly Asp Val Asp Pro Met Arg Val Ala Glu Gly Arg Ser
165 170 175
Leu Gly Asp Leu Glu Thr Ile His Tyr Gly Leu Ile Pro Arg Ala Asn
180 185 190
Arg Gly Ile Val Ala Ile Asn Glu Leu Pro Asp Leu Ala Glu Arg Ile
195 200 205
Gln Val Ala Met Leu Asn Val Met Glu Glu Arg Asp Val Gln Ile Arg
210 215 220
Gly Tyr Asn Ile Arg Leu Asp Leu Asp Val Leu Val Val Ala Ser Ala
225 230 235 240
Asn Pro Glu Asp Tyr Thr Asn Arg Gly Arg Ile Ile Thr Pro Leu Lys
245 250 255
Asp Arg Phe Gly Ala Glu Ile Arg Thr His Tyr Pro Leu Glu Leu Asp
260 265 270
Asp Glu Val Ala Ile Ile Arg Gln Glu Ala Glu Leu Val Ala Gln Val
275 280 285
Pro Asp Ile Leu Val Glu Ile Leu Ala Arg Tyr Thr Arg Ala Leu Arg
290 295 300
Glu Ser Ser Ser Val Asn Gln Arg Ser Gly Val Ser Ala Arg Phe Ser
305 310 315 320
Ile Ala Gly Ala Glu Thr Val Ala Ala Ala Ala Leu Arg Arg Ala Ala
325 330 335
Val Phe Gly Glu Asp Glu Ala Val Ala Arg Leu Val Asp Leu Glu Ala
340 345 350
Ala Val Glu Val Leu Gly Gly Lys Ile Glu Phe Glu Ser Gly Glu Glu
355 360 365
Gly Arg Glu Trp Glu Ile Leu Asp Tyr Leu Leu Arg Thr Ala Thr Ala
370 375 380
Glu Ala Leu Arg Ser Thr Leu Arg Ser Leu Asp Leu Thr Pro Leu Ile
385 390 395 400
Ala Ala Leu Asp Gly Ser Ile Thr Val Ser Thr Gly Thr Asn Ile Thr
405 410 415
Ala Ser Glu Phe Leu Ala Ser Leu Pro Glu Leu Gly Glu Ser Thr Leu
420 425 430
Tyr Asp Asp Ile Ala Gln Ala Phe Gly Ala Thr Thr Pro Ser Thr Arg
435 440 445
Ala Met Ala Ile Glu Leu Ala Leu Glu Gly Leu Tyr Leu Ser Arg Lys
450 455 460
Ile Ala Lys Asp Ser Gly Glu Gly Glu Thr Ile Tyr Gly
465 470 475
<210>247
<211>472
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(442)
<223>RXA02138
<400>247
tcactcgccg gagatccgta aaaaggggag tacactgcaa gcctatgatg tcccggccta 60
aaagtaattc aaagggccaa cagttaagga gactaaagcg atg acc gct cca tca 115
Met Thr Ala Pro Ser
1 5
acc aac acc ggt gtt atc ttg acc gag tcc gca gcg tcc aaa gct aag 163
Thr Asn Thr Gly Val Ile Leu Thr Glu Ser Ala Ala Ser Lys Ala Lys
10 15 20
gca ctc atc gat cag gaa ggc cgc gac gac ctc tct ctg cgt atc gcc 211
Ala Leu Ile Asp Gln Glu Gly Arg Asp Asp Leu Ser Leu Arg Ile Ala
25 30 35
gtt cag cct ggc ggc tgc tct ggc ctt cgt tac cag ctt tac ttc gac 259
Val Gln Pro Gly Gly Cys Ser Gly Leu Arg Tyr Gln Leu Tyr Phe Asp
40 45 50
gac cgc acc ctt gat ggc gat aag gaa gac atc gtc ggt ggc gtt cgc 307
Asp Arg Thr Leu Asp Gly Asp Lys Glu Asp Ile Val Gly Gly Val Arg
55 60 65
ctt gtc gtt gac aag atg agc acc cca tac ttg ctc ggc gct cag atc 355
Leu Val Val Asp Lys Met Ser Thr Pro Tyr Leu Leu Gly Ala Gln Ile
70 75 80 85
gac ttc gct gac acc atc gag cag cag ggc ttc acc atc gac aac cca 403
Asp Phe Ala Asp Thr Ile Glu Gln Gln Gly Phe Thr Ile Asp Asn Pro
90 95 100
aac gca ggc agc tct tgc gct tgt ggt gac tcc ttc aac taaagagatt 452
Asn Ala Gly Ser Ser Cys Ala Cys Gly Asp Ser Phe Asn
105 110
ccgttatgta ggaataaagg 472
<210>248
<211>114
<212>PRT
<213>谷氨酸棒状杆菌
<400>248
Met Thr Ala Pro Ser Thr Asn Thr Gly Val Ile Leu Thr Glu Ser Ala
1 5 10 15
Ala Ser Lys Ala Lys Ala Leu Ile Asp Gln Glu Gly Arg Asp Asp Leu
20 25 30
Ser Leu Arg Ile Ala Val Gln Pro Gly Gly Cys Ser Gly Leu Arg Tyr
35 40 45
Gln Leu Tyr Phe Asp Asp Arg Thr Leu Asp Gly Asp Lys Glu Asp Ile
50 55 60
Val Gly Gly Val Arg Leu Val Val Asp Lys Met Ser Thr Pro Tyr Leu
65 70 75 80
Leu Gly Ala Gln Ile Asp Phe Ala Asp Thr Ile Glu Gln Gln Gly Phe
85 90 95
Thr Ile Asp Asn Pro Asn Ala Gly Ser Ser Cys Ala Cys Gly Asp Ser
100 105 110
Phe Asn
<210>249
<211>1381
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1351)
<223>RXA02151
<400>249
ttcaagattt aactaattgt taatcttttt ggcatacatc gagtcggacg gttgcgctgc 60
gaaatgagcg catcgtcttt tttaggagtt gtccgccacc gtg aaa act gga aac 115
Val Lys Thr Gly Asn
1 5
tta aac cca gcc cca cag cag gtg acg gct ccc cag aac aag gct cgt 163
Leu Asn Pro Ala Pro Gln Gln Val Thr Ala Pro Gln Asn Lys Ala Arg
10 15 20
atg gat tgg cca gac atc gcc aag gga ata tcc atc cta ggt gtc gtg 211
Met Asp Trp Pro Asp Ile Ala Lys Gly Ile Ser Ile Leu Gly Val Val
25 30 35
tta cta cac gtg tcg ttg gca att cca ggt ggc cag gac acc atg atg 259
Leu Leu His Val Ser Leu Ala Ile Pro Gly Gly Gln Asp Thr Met Met
40 45 50
tcc cac ctg aac gca ctg ctt gat cca ctt cgg atg cca tta ttt ttt 307
Ser His Leu Asn Ala Leu Leu Asp Pro Leu Arg Met Pro Leu Phe Phe
55 60 65
atg gtg agt gga ttt ttt gca gtt aaa gtt ctg aat caa agc ttt ggt 355
Met Val Ser Gly Phe Phe Ala Val Lys Val Leu Asn Gln Ser Phe Gly
70 75 80 85
gaa ctt ttc cgc ggg cga ctg tgg ttc tac ctg gtt cca tat ttg ctg 403
Glu Leu Phe Arg Gly Arg Leu Trp Phe Tyr Leu Val Pro Tyr Leu Leu
90 95 100
tgg act cca gtg aat ctt tat cta cac cgc ctc gag ggc aca gtt ttt 451
Trp Thr Pro Val Asn Leu Tyr Leu His Arg Leu Glu Gly Thr Val Phe
105 110 115
acc ggt aga gca ccg gga aca tgg gaa tgg tac agc ggc tcg atg ctc 499
Thr Gly Arg Ala Pro Gly Thr Trp Glu Trp Tyr Ser Gly Ser Met Leu
120 125 130
tcg gcc acc aat atg tac tgg ttc ctc tac ttc ttg gtc atc ttc aac 547
Ser Ala Thr Asn Met Tyr Trp Phe Leu Tyr Phe Leu Val Ile Phe Asn
135 140 145
cta ttt tta tgg gca acg aga aaa ctc cca gct tgg gca att gtg gcg 595
Leu Phe Leu Trp Ala Thr Arg Lys Leu Pro Ala Trp Ala Ile Val Ala
150 155 160 165
ttg gtg gcc tca ctg tgg cta ctt atg ccg gct tat agc gag att gag 643
Leu Val Ala Ser Leu Trp Leu Leu Met Pro Ala Tyr Ser Glu Ile Glu
170 175 180
att cta cgc aag tcc att att tac ttg cct aca ttc ctc att ggc gct 691
Ile Leu Arg Lys Ser Ile Ile Tyr Leu Pro Thr Phe Leu Ile Gly Ala
185 190 195
tac ttc cgc cca ctg att tcg cgt ttt gca gaa gcc gca aca agg cca 739
Tyr Phe Arg Pro Leu Ile Ser Arg Phe Ala Glu Ala Ala Thr Arg Pro
200 205 210
aaa gca ata gtg ttt gca gcg gtc ctt tat gtc tcg gga ctt gct ttg 787
Lys Ala Ile Val Phe Ala Ala Val Leu Tyr Val Ser Gly Leu Ala Leu
215 220 225
ggc gtg atc tca aat ggg ctg cgc gac age gaa aac cat ggc gca agc 835
Gly Val Ile Ser Asn Gly Leu Arg Asp Ser Glu Asn His Gly Ala Ser
230 235 240 245
gtg ctg tgg ctg atg aac ctc cgc gat act ttt gct cat gca ctc ggc 883
Val Leu Trp Leu Met Asn Leu Arg Asp Thr Phe Ala His Ala Leu Gly
250 255 260
ggc aac ctc act gga ttc gat atg gat cac ctt cct gga atg atc att 931
Gly Asn Leu Thr Gly Phe Asp Met Asp His Leu Pro Gly Met Ile Ile
265 270 275
cgg att gtt tcc ctg cct gca gga att gtg ttg tgc gta tgg ctt ggc 979
Arg Ile Val Ser Leu Pro Ala Gly Ile Val Leu Cys Val Trp Leu Gly
280 285 290
cga ata aag cca gta ggg gag ttt ttg aaa ctt att ggt agg cac acc 1027
Arg Ile Lys Pro Val Gly Glu Phe Leu Lys Leu Ile Gly Arg His Thr
295 300 305
ctt ccc atc tac att ggg cat gca aca gga cta tcg ctg att ttt ggt 1075
Leu Pro Ile Tyr Ile Gly His Ala Thr Gly Leu Ser Leu Ile Phe Gly
310 315 320 325
ttc ggc ttg cgc tgg aat ttc atg gag att gat aac ttc tct gac agt 1123
Phe Gly Leu Arg Trp Asn Phe Met Glu Ile Asp Asn Phe Ser Asp Ser
330 335 340
ttg tgg cac cac acc aat acg tgg atg gtc atc gcg ttt gcc tgc gcg 1171
Leu Trp His His Thr Asn Thr Trp Met Val Ile Ala Phe Ala Cys Ala
345 350 355
atg ctc ggc ggg tac ctg acc tat ctg atc tcg cga gtt cca gtg ctg 1219
Met Leu Gly Gly Tyr Leu Thr Tyr Leu Ile Ser Arg Val Pro Val Leu
360 365 370
gga tgg act ctt gtt ccc cct aaa ctg cca gaa cca gat aaa act cca 1267
Gly Trp Thr Leu Val Pro Pro Lys Leu Pro Glu Pro Asp Lys Thr Pro
375 380 385
gct aaa gca caa gct gat tct cac gtt aaa gct cag tct gcg aag cct 1315
Ala Lys Ala Gln Ala Asp Ser His Val Lys Ala Gln Ser Ala Lys Pro
390 395 400 405
atg aat gct tct acc tct tct aag acg tac ggt ata taactgaagc 1361
Met Asn Ala Ser Thr Ser Ser Lys Thr Tyr Gly Ile
410 415
ataacctgtg tgattctgct 1381
<210>250
<211>417
<212>PRT
<213>谷氨酸棒状杆菌
<400>250
Val Lys Thr Gly Asn Leu Asn Pro Ala Pro Gln Gln Val Thr Ala Pro
l 5 10 15
Gln Asn Lys Ala Arg Met Asp Trp Pro Asp Ile Ala Lys Gly Ile Ser
20 25 30
Ile Leu Gly Val Val Leu Leu His Val Ser Leu Ala Ile Pro Gly Gly
35 40 45
Gln Asp Thr Met Met Ser His Leu Asn Ala Leu Leu Asp Pro Leu Arg
50 55 60
Met Pro Leu Phe Phe Met Val Ser Gly Phe Phe Ala Val Lys Val Leu
65 70 75 80
Asn Gln Ser Phe Gly Glu Leu Phe Arg Gly Arg Leu Trp Phe Tyr Leu
85 90 95
Val Pro Tyr Leu Leu Trp Thr Pro Val Asn Leu Tyr Leu His Arg Leu
100 105 110
Glu Gly Thr Val Phe Thr Gly Arg Ala Pro Gly Thr Trp Glu Trp Tyr
115 120 125
Ser Gly Ser Met Leu Ser Ala Thr Asn Met Tyr Trp Phe Leu Tyr Phe
130 135 140
Leu Val Ile Phe Asn Leu Phe Leu Trp Ala Thr Arg Lys Leu Pro Ala
145 150 155 160
Trp Ala Ile Val Ala Leu Val Ala Ser Leu Trp Leu Leu Met Pro Ala
165 170 175
Tyr Ser Glu Ile Glu Ile Leu Arg Lys Ser Ile Ile Tyr Leu Pro Thr
180 185 190
Phe Leu Ile Gly Ala Tyr Phe Arg Pro Leu Ile Ser Arg Phe Ala Glu
195 200 205
Ala Ala Thr Arg Pro Lys Ala Ile Val Phe Ala Ala Val Leu Tyr Val
210 215 220
Ser Gly Leu Ala Leu Gly Val Ile Ser Asn Gly Leu Arg Asp Ser Glu
225 230 235 240
Asn His Gly Ala Ser Val Leu Trp Leu Met Asn Leu Arg Asp Thr Phe
245 250 255
Ala His Ala Leu Gly Gly Asn Leu Thr Gly Phe Asp Met Asp His Leu
260 265 270
Pro Gly Met Ile Ile Arg Ile Val Ser Leu Pro Ala Gly Ile Val Leu
275 280 285
Cys Val Trp Leu Gly Arg Ile Lys Pro Val Gly Glu Phe Leu Lys Leu
290 295 300
Ile Gly Arg His Thr Leu Pro Ile Tyr Ile Gly His Ala Thr Gly Leu
305 310 315 320
Ser Leu Ile Phe Gly Phe Gly Leu Arg Trp Asn Phe Met Glu Ile Asp
325 330 335
Asn Phe Ser Asp Ser Leu Trp His His Thr Asn Thr Trp Met Val Ile
340 345 350
Ala Phe Ala Cys Ala Met Leu Gly Gly Tyr Leu Thr Tyr Leu Ile Ser
355 360 365
Arg Val Pro Val Leu Gly Trp Thr Leu Val Pro Pro Lys Leu Pro Glu
370 375 380
Pro Asp Lys Thr Pro Ala Lys Ala Gln Ala Asp Ser His Val Lys Ala
385 390 395 400
Gln Ser Ala Lys Pro Met Asn Ala Ser Thr Ser Ser Lys Thr Tyr Gly
405 410 415
Ile
<210>251
<211>1027
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(997)
<223>RXA02163
<400>251
cttgaccgag gtcagagcag taattggctc ggaaaagaag gaattcttcg aagatccaga 60
agtcgttgaa gccttcgccg accacggctt tgaagttaac gtg gac acc gca gga 115
Val Asp Thr Ala Gly
1 5
tct cgt cgg atc gcc act gat gtg gac ttg agc ccg tac gat ttc gcg 163
Ser Arg Arg Ile Ala Thr Asp Val Asp Leu Ser Pro Tyr Asp Phe Ala
10 15 20
ttc cca tcc tct gca cca gct gcg cag aag atc tcc gaa gcc aac acc 211
Phe Pro Ser Ser Ala Pro Ala Ala Gln Lys Ile Ser Glu Ala Asn Thr
25 30 35
acg acg ggc cga ttc acc cca ttc tat tcg cct atg gcg gta gca aca 259
Thr Thr Gly Arg Phe Thr Pro Phe Tyr Ser Pro Met Ala Val Ala Thr
40 45 50
ttc gga acc atc acc gac atg ttg gaa acc aag ggg atc gtg agc aat 307
Phe Gly Thr Ile Thr Asp Met Leu Glu Thr Lys Gly Ile Val Ser Asn
55 60 65
gta ggc ggc acc gac acc atc gat ttg ggc gcg ttg ttg aat gaa acc 355
Val Gly Gly Thr Asp Thr Ile Asp Leu Gly Ala Leu Leu Asn Glu Thr
70 75 80 85
caa gcc gga acc cgc tgg agc gaa ctt agc ccc aac tat cag tcc aac 403
Gln Ala Gly Thr Arg Trp Ser Glu Leu Ser Pro Asn Tyr Gln Ser Asn
90 95 100
cgc gtc gtg cag att tct acc acc gat gtg cgc acc tct aac tcg gcg 451
Arg Val Val Gln Ile Ser Thr Thr Asp Val Arg Thr Ser Asn Ser Ala
105 110 115
gcc atg tac cta tca atg atg tcc tgg gtg aaa aat ggc gga aaa acg 499
Ala Met Tyr Leu Ser Met Met Ser Trp Val Lys Asn Gly Gly Lys Thr
120 125 130
gtg agc agc act gcg gaa gcc gat gcc atc atc cca gag ctt agc caa 547
Val Ser Ser Thr Ala Glu Ala Asp Ala Ile Ile Pro Glu Leu Ser Gln
135 140 145
ctg ttc gtg ggc cag ggc tat acc gaa agc acc tcc gcc ggc ccg ttt 595
Leu Phe Val Gly Gln Gly Tyr Thr Glu Ser Thr Ser Ala Gly Pro Phe
150 155 160 165
gat gaa tac ctc tcc caa gga atg ggt tct aaa cca atg gtg atg atc 643
Asp Glu Tyr Leu Ser Gln Gly Met Gly Ser Lys Pro Met Val Met Ile
170 175 180
tac gaa gcc cag ttc ctc gcg gaa caa aac aag gaa aac tca cgg att 691
Tyr Glu Ala Gln Phe Leu Ala Glu Gln Asn Lys Glu Asn Ser Arg Ile
185 190 195
tcc ggc gat atg gaa cta gtg tat cca agc ccc acc gtg tac agc acg 739
Ser Gly Asp Met Glu Leu Val Tyr Pro Ser Pro Thr Val Tyr Ser Thr
200 205 210
cat acg gtg gtt agc ttg agt gac gtc ggc gcg gag atc ggc gaa ctc 787
His Thr Val Val Ser Leu Ser Asp Val Gly Ala Glu Ile Gly Glu Leu
215 220 225
cta gaa acc gac gaa acc ctg cag cag ttg gca gtc aaa cac ggt ttt 835
Leu Glu Thr Asp Glu Thr Leu Gln Gln Leu Ala Val Lys His Gly Phe
230 235 240 245
agg cca aag aac tcc gca atg atc gcc gat gct ggc atg acc gac cgc 883
Arg Pro Lys Asn Ser Ala Met Ile Ala Asp Ala Gly Met Thr Asp Arg
250 255 260
atg ccc aac aac ctc aat gtc att gat ccg ccg gac tat gac ttc cta 931
Met Pro Asn Asn Leu Asn Val Ile Asp Pro Pro Asp Tyr Asp Phe Leu
265 270 275
gaa cga ctc att gat ggc gtg ggc gca tcg tac agt gcc acc cca gca 979
Glu Arg Leu Ile Asp Gly Val Gly Ala Ser Tyr Ser Ala Thr Pro Ala
280 285 290
gaa gag gac aca gat cta tgaaaaatct tgtaaaaggt acagcgctgg 1027
Glu Glu Asp Thr Asp Leu
295
<210>252
<211>299
<212>PRT
<213>谷氨酸棒状杆菌
<400>252
Val Asp Thr Ala Gly Ser Arg Arg Ile Ala Thr Asp Val Asp Leu Ser
1 5 10 15
Pro Tyr Asp Phe Ala Phe Pro Ser Ser Ala Pro Ala Ala Gln Lys Ile
20 25 30
Ser Glu Ala Asn Thr Thr Thr Gly Arg Phe Thr Pro Phe Tyr Ser Pro
35 40 45
Met Ala Val Ala Thr Phe Gly Thr Ile Thr Asp Met Leu Glu Thr Lys
50 55 60
Gly Ile Val Ser Asn Val Gly Gly Thr Asp Thr Ile Asp Leu Gly Ala
65 70 75 80
Leu Leu Asn Glu Thr Gln Ala Gly Thr Arg Trp Ser Glu Leu Ser Pro
85 90 95
Asn Tyr Gln Ser Asn Arg Val Val Gln Ile Ser Thr Thr Asp Val Arg
100 105 110
Thr Ser Asn Ser Ala Ala Met Tyr Leu Ser Met Met Ser Trp Val Lys
115 120 125
Asn Gly Gly Lys Thr Val Ser Ser Thr Ala Glu Ala Asp Ala Ile Ile
130 135 140
Pro Glu Leu Ser Gln Leu Phe Val Gly Gln Gly Tyr Thr Glu Ser Thr
145 150 155 160
Ser Ala Gly Pro Phe Asp Glu Tyr Leu Ser Gln Gly Met Gly Ser Lys
165 170 175
Pro Met Val Met Ile Tyr Glu Ala Gln Phe Leu Ala Glu Gln Asn Lys
180 185 190
Glu Asn Ser Arg Ile Ser Gly Asp Met Glu Leu Val Tyr Pro Ser Pro
195 200 205
Thr Val Tyr Ser Thr His Thr Val Val Ser Leu Ser Asp Val Gly Ala
210 215 220
Glu Ile Gly Glu Leu Leu Glu Thr Asp Glu Thr Leu Gln Gln Leu Ala
225 230 235 240
Val Lys His Gly Phe Arg Pro Lys Asn Ser Ala Met Ile Ala Asp Ala
245 250 255
Gly Met Thr Asp Arg Met Pro Asn Asn Leu Asn Val Ile Asp Pro Pro
260 265 270
Asp Tyr Asp Phe Leu Glu Arg Leu Ile Asp Gly Val Gly Ala Ser Tyr
275 280 285
Ser Ala Thr Pro Ala Glu Glu Asp Thr Asp Leu
290 295
<210>253
<211>709
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(679)
<223>RXA02185
<400>253
caactccttg gggtgaagcc agacatccac tggcagagca actcctccgc tctaacccga 60
cagctaacct cgacggcgac aaatgagagg aaaacttttc atg gga cgt cac tcc 115
Met Gly Arg His Ser
1 5
act aag act agc tcc gcg ttc acc aag ctc gca gct tcc acc atc gct 163
Thr Lys Thr Ser Ser Ala Phe Thr Lys Leu Ala Ala Ser Thr Ile Ala
10 15 20
ttc ggt gct gct gca acc atc atg gct cct tct gca tct gct gca cct 211
Phe Gly Ala Ala Ala Thr Ile Met Ala Pro Ser Ala Ser Ala Ala Pro
25 30 35
gat tcc gac tgg gat cgc ctc gca cag tgc gag tcc ggt ggt aac tgg 259
Asp Ser Asp Trp Asp Arg Leu Ala Gln Cys Glu Ser Gly Gly Asn Trp
40 45 50
gca atc aac acc ggt aac ggc tac cac ggt ggt ctg cag ttc tcc gct 307
Ala Ile Asn Thr Gly Asn Gly Tyr His Gly Gly Leu Gln Phe Ser Ala
55 60 65
agc acc tgg gct gct tac ggc ggc cag gag ttc gct acc tac gca tac 355
Ser Thr Trp Ala Ala Tyr Gly Gly Gln Glu Phe Ala Thr Tyr Ala Tyr
70 75 80 85
cag gca acc cgt gag cag cag atc gct gtt gca gag cgc acc ttg gct 403
Gln Ala Thr Arg Glu Gln Gln Ile Ala Val Ala Glu Arg Thr Leu Ala
90 95 100
ggt cag ggc tgg ggc gca tgg cct gct tgc tcc gct tcc ctt gga ctg 451
Gly Gln Gly Trp Gly Ala Trp Pro Ala Cys Ser Ala Ser Leu Gly Leu
105 110 115
aac tcc gct cca acc cag cgt gac ctc tcc gct acc acc tcc acc cca 499
Asn Ser Ala Pro Thr Gln Arg Asp Leu Ser Ala Thr Thr Ser Thr Pro
120 125 130
gag cca gct gca gct gca cca gct gtt gct gag tac aac gct cct gca 547
Glu Pro Ala Ala Ala Ala Pro Ala Val Ala Glu Tyr Asn Ala Pro Ala
135 140 145
gcc aac atc gca gtt ggc tcc acc gac ttg aac acc atc aag tcc acc 595
Ala Asn Ile Ala Val Gly Ser Thr Asp Leu Asn Thr Ile Lys Ser Thr
150 155 160 165
tac ggc gct gtc acc ggc acc ctc gct cag tac ggc atc acc gtt cca 643
Tyr Gly Ala Val Thr Gly Thr Leu Ala Gln Tyr Gly Ile Thr Val Pro
170 175 180
gct gag gtt gag tct tac tac aac gct ttc gtc ggc taaatctagc 689
Ala Glu Val Glu Ser Tyr Tyr Asn Ala Phe Val Gly
185 190
tgcacttttt aaaagggagg 709
<210>254
<211>193
<212>PRT
<213>谷氨酸棒状杆菌
<400>254
Met Gly Arg His Ser Thr Lys Thr Ser Ser Ala Phe Thr Lys Leu Ala
1 5 10 15
Ala Ser Thr Ile Ala Phe Gly Ala Ala Ala Thr Ile Met Ala Pro Ser
20 25 30
Ala Ser Ala Ala Pro Asp Ser Asp Trp Asp Arg Leu Ala Gln Cys Glu
35 40 45
Ser Gly Gly Asn Trp Ala Ile Asn Thr Gly Asn Gly Tyr His Gly Gly
50 55 60
Leu Gln Phe Ser Ala Ser Thr Trp Ala Ala Tyr Gly Gly Gln Glu Phe
65 70 75 80
Ala Thr Tyr Ala Tyr Gln Ala Thr Arg Glu Gln Gln Ile Ala Val Ala
85 90 95
Glu Arg Thr Leu Ala Gly Gln Gly Trp Gly Ala Trp Pro Ala Cys Ser
100 105 110
Ala Ser Leu Gly Leu Asn Ser Ala Pro Thr Gln Arg Asp Leu Ser Ala
115 120 125
Thr Thr Ser Thr Pro Glu Pro Ala Ala Ala Ala Pro Ala Val Ala Glu
130 135 140
Tyr Asn Ala Pro Ala Ala Asn Ile Ala Val Gly Ser Thr Asp Leu Asn
145 150 155 160
Thr Ile Lys Ser Thr Tyr Gly Ala Val Thr Gly Thr Leu Ala Gln Tyr
165 170 175
Gly Ile Thr Val Pro Ala Glu Val Glu Ser Tyr Tyr Asn Ala Phe Val
180 185 190
Gly
<210>255
<211>328
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(298)
<223>RXA02186
<400>255
tccaaccacc ccttgagggt ggggatgggg gagtcttttt tcatgatgtt gttaagttta 60
agccttgttg aggtgacttt tgttcccaga gtttgaaaga atg tat gac atg gca 115
Met Tyr Asp Met Ala
1 5
aac gta gag aag aag cac ttc gtc gat ccg gca tgg ccg gag cac aat 163
Asn Val Glu Lys Lys His Phe Val Asp Pro Ala Trp Pro Glu His Asn
10 15 20
cca gct gac gga cac gtc gtt act gaa ctc atc tcc aag gtc gca ggc 211
Pro Ala Asp Gly His Val Val Thr Glu Leu Ile Ser Lys Val Ala Gly
25 30 35
gcg tcc agc cca tgg ggc gat gac aag gaa ttc cca gtt tct gca gaa 259
Ala Ser Ser Pro Trp Gly Asp Asp Lys Glu Phe Pro Val Ser Ala Glu
40 45 50
gag acc gga tac gtt cac ccg tac acc cgg atc aac cgc taagaaactt 308
Glu Thr Gly Tyr Val His Pro Tyr Thr Arg Ile Asn Arg
55 60 65
aaaaaagagg caacccgttt 328
<210>256
<211>66
<212>PRT
<213>谷氨酸棒状杆菌
<400>256
Met Tyr Asp Met Ala Asn Val Glu Lys Lys His Phe Val Asp Pro Ala
1 5 10 15
Trp Pro Glu His Asn Pro Ala Asp Gly His Val Val Thr Glu Leu Ile
20 25 30
Ser Lys Val Ala Gly Ala Ser Ser Pro Trp Gly Asp Asp Lys Glu Phe
35 40 45
Pro Val Ser Ala Glu Glu Thr Gly Tyr Val His Pro Tyr Thr Arg Ile
50 55 60
Asn Arg
65
<210>257
<211>508
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(478)
<223>RXA02203
<400>257
cgattcaaaa gagttcaccg aggtaggaaa catcgtggtc gcagccattg atgccctcct 60
gggcgtgcag ctctaggaaa tttcaggaac aatacatctc atg acc att cgc gcc 115
Met Thr Ile Arg Ala
1 5
act ttc cag cct tcc gta gat gaa ttc atc tcc act ctc gaa gag ttt 163
Thr Phe Gln Pro Ser Val Asp Glu Phe Ile Ser Thr Leu Glu Glu Phe
10 15 20
gca aca ggt tca tac ctt aaa gag gac gaa aag gaa ttc tgg gac gaa 211
Ala Thr Gly Ser Tyr Leu Lys Glu Asp Glu Lys Glu Phe Trp Asp Glu
25 30 35
cct ttc gat gtc aag gca ctt cca gat ctt cgt ttc atc ttg gaa aac 259
Pro Phe Asp Val Lys Ala Leu Pro Asp Leu Arg Phe Ile Leu Glu Asn
40 45 50
tac ctt gat tcc cta gat aag ctc ggt gaa gca cca gac ctt gat gct 307
Tyr Leu Asp Ser Leu Asp Lys Leu Gly Glu Ala Pro Asp Leu Asp Ala
55 60 65
gtt aac gcc tcc gct cag tcc acc ctt gat gag ctg gag aag ttc aac 355
Val Asn Ala Ser Ala Gln Ser Thr Leu Asp Glu Leu Glu Lys Phe Asn
70 75 80 85
aca aag cac cac ggt gct gtt gtt gag cct gaa gaa aaa gaa gag atc 403
Thr Lys His His Gly Ala Val Val Glu Pro Glu Glu Lys Glu Glu Ile
90 95 100
act aaa ttg atg ttt gat gct gca aag cag act ggt gca gat gat ctt 451
Thr Lys Leu Met Phe Asp Ala Ala Lys Gln Thr Gly Ala Asp Asp Leu
105 110 115
tct gca gag gct ttc cct gag ttt gag taaatttcag tagaagtttt 498
Ser Ala Glu Ala Phe Pro Glu Phe Glu
120 125
ttagcgccat 508
<210>258
<211>126
<212>PRT
<213>谷氨酸棒状杆菌
<400>258
Met Thr Ile Arg Ala Thr Phe Gln Pro Ser Val Asp Glu Phe Ile Ser
1 5 10 15
Thr Leu Glu Glu Phe Ala Thr Gly Ser Tyr Leu Lys Glu Asp Glu Lys
20 25 30
Glu Phe Trp Asp Glu Pro Phe Asp Val Lys Ala Leu Pro Asp Leu Arg
35 40 45
Phe Ile Leu Glu Asn Tyr Leu Asp Ser Leu Asp Lys Leu Gly Glu Ala
50 55 60
Pro Asp Leu Asp Ala Val Asn Ala Ser Ala Gln Ser Thr Leu Asp Glu
65 70 75 80
Leu Glu Lys Phe Asn Thr Lys His His Gly Ala Val Val Glu Pro Glu
85 90 95
Glu Lys Glu Glu Ile Thr Lys Leu Met Phe Asp Ala Ala Lys Gln Thr
100 105 110
Gly Ala Asp Asp Leu Ser Ala Glu Ala Phe Pro Glu Phe Glu
115 120 125
<210>259
<211>1198
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1168)
<223>RXA02206
<400>259
gccggcatcg gcaccccaga acagctcaga gatgattcgg aggtgtccgc tggagctgat 60
cggcaggaac tctgtgaggc cttgaacgat ggagagcaca atg gtt tgt acc cag 115
Met Val Cys Thr Gln
1 5
cca ata ttt tca gtt gcg gca gga tct gct gct gcg gct agg agg gtt 163
Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala Ala Ala Arg Arg Val
10 15 20
atc tct tca ttc acc cga tct acc gta cta cct tat gac ctc agt agt 211
Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro Tyr Asp Leu Ser Ser
25 30 35
gtg gtg ggc gtg aaa cag cga atg gtc ggt tca agt ggt ttg cgg gta 259
Val Val Gly Val Lys Gln Arg Met Val Gly Ser Ser Gly Leu Arg Val
40 45 50
tcc agg ctc ggt ttg ggc acc tca aca tgg ggc tcg ggc acc gag ctg 307
Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly Ser Gly Thr Glu Leu
55 60 65
gct gag gca ggc gat atc ttt aag gcg ttc atc aat tct ggt ggc acg 355
Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile Asn Ser Gly Gly Thr
70 75 80 85
ctt atc gac gtc tcc ccc aac tac acc acc ggc gtc gcg gaa gaa atg 403
Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly Val Ala Glu Glu Met
90 95 100
ctc ggc acg atg ttg gat gcg gaa gtc tct cgt tcg gct gtc gtc att 451
Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg Ser Ala Val Val Ile
105 110 115
tcc tcc agc gca ggt gtc aac ccc gct ctg ccg ctc ggc cga cgt gtg 499
Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro Leu Gly Arg Arg Val
120 125 130
gat tgc tcc cgc cgc aat ttg att gcc caa tta gat gtc acc ctg cgg 547
Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu Asp Val Thr Leu Arg
135 140 145
gca tta aac act gac tat ttg gat ttg tgg tct gtg ggc tat tgg gat 595
Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser Val Gly Tyr Trp Asp
150 155 160 165
gag ggc acc cca ccg cat gag gtg gcc gat act ttg gat tac gcc gtg 643
Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr Leu Asp Tyr Ala Val
170 175 180
cgc acc ggc cga gtc cga tat gcc ggt gtc cga gga tat tcc ggt tgg 691
Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg Gly Tyr Ser Gly Trp
185 190 195
cag tta gcg gtc acc cac gct gca tcc aat cat gca gcg gcc tcc gcc 739
Gln Leu Ala Val Thr His Ala Ala Ser Asn His Ala Ala Ala Ser Ala
200 205 210
cgc ccc gtg gtc gtt gca caa aat gaa tac agc ctg ctg gaa cgc cgc 787
Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser Leu Leu Glu Arg Arg
215 220 225
gca gaa caa gaa ctc ctc cct gcc acc caa cac cta ggt gtc gga ttc 835
Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His Leu Gly Val Gly Phe
230 235 240 245
ttt gct ggc gct ccg ctg ggg caa ggc gtg ctg act gct aaa tac cgc 883
Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu Thr Ala Lys Tyr Arg
250 255 260
tcc gaa att ccc cat gat tcc aga gct gca tcc aca gga cgc gac gca 931
Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser Thr Gly Arg Asp Ala
265 270 275
gaa gtc caa agc tac cta gat aat cga ggc cgc atc att gtc gat gct 979
Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg Ile Ile Val Asp Ala
280 285 290
ctt gat act gca gcc aaa gga tta ggc att agc ccc gct gtc aca gcc 1027
Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser Pro Ala Val Thr Ala
295 300 305
acc acc tgg gtg cgt gat cgt ccc gga gtg aca gct gtc atc gtg ggc 1075
Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr Ala Val Ile Val Gly
310 315 320 325
gct cgc aca cat gaa cag ctg tca cat ctt ctc aag gcg gaa tcg gtg 1123
Ala Arg Thr His Glu Gln Leu Ser His Leu Leu Lys Ala Glu Ser Val
330 335 340
act ttg cca aca cca atc aca caa gcc ctt gat gat gtc tcc ctg 1168
Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp Asp Val Ser Leu
345 350 355
tgacttggtc caattacatt cactggtaat 1198
<210>260
<211>356
<212>PRT
<213>谷氨酸棒状杆菌
<400>260
Met Val Cys Thr Gln Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala
1 5 10 15
Ala Ala Arg Arg Val Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro
20 25 30
Tyr Asp Leu Ser Ser Val Val Gly Val Lys Gln Arg Met Val Gly Ser
35 40 45
Ser Gly Leu Arg Val Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly
50 55 60
Ser Gly Thr Glu Leu Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile
65 70 75 80
Asn Ser Gly Gly Thr Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly
85 90 95
Val Ala Glu Glu Met Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg
100 105 110
Ser Ala Val Val Ile Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro
115 120 125
Leu Gly Arg Arg Val Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu
130 135 140
Asp Val Thr Leu Arg Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser
145 150 155 160
Val Gly Tyr Trp Asp Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr
165 170 175
Leu Asp Tyr Ala Val Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg
180 185 190
Gly Tyr Ser Gly Trp Gln Leu Ala Val Thr His Ala Ala Ser Asn His
195 200 205
Ala Ala Ala Ser Ala Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser
210 215 220
Leu Leu Glu Arg Arg Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His
225 230 235 240
Leu Gly Val Gly Phe Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu
245 250 255
Thr Ala Lys Tyr Arg Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser
260 265 270
Thr Gly Arg Asp Ala Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg
275 280 285
Ile Ile Val Asp Ala Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser
290 295 300
Pro Ala Val Thr Ala Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr
305 310 315 320
Ala Val Ile Val Gly Ala Arg Thr His Glu Gln Leu Ser His Leu Leu
325 330 335
Lys Ala Glu Ser Val Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp
340 345 350
Asp Val Ser Leu
355
<210>261
<211>802
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(772)
<223>RXA02217
<400>261
tccgcggccc caaatgggta tgggcgcttt tgaccatcgt caatggtgtt ggccccgctg 60
cctactgggc tttcggcagg aaaaactaag agttgttagg gtg gcg ctc atg act 115
Val Ala Leu Met Thr
1 5
aat aaa aca cga gct cta ctc att ggt ggc cac ggc aag gtg gcc ctc 163
Asn Lys Thr Arg Ala Leu Leu Ile Gly Gly His Gly Lys Val Ala Leu
10 15 20
cta gca acc ccc atg ctt atc gac gcc tcg gtg cag gtc act tcc atg 211
Leu Ala Thr Pro Met Leu Ile Asp Ala Ser Val Gln Val Thr Ser Met
25 30 35
tac cgc aat ccg gac cac agg tcc gaa att gag gcg ctg ggc gcc aca 259
Tyr Arg Asn Pro Asp His Arg Ser Glu Ile Glu Ala Leu Gly Ala Thr
40 45 50
act tta gag cgt gac gtc acc aca ctc agc gtg gag gat tgg gca gat 307
Thr Leu Glu Arg Asp Val Thr Thr Leu Ser Val Glu Asp Trp Ala Asp
55 60 65
ctg ctc aag gac ttc gac gta gtg gtg tgg agc gcc gga aac ggt ggc 355
Leu Leu Lys Asp Phe Asp Val Val Val Trp Ser Ala Gly Asn Gly Gly
70 75 80 85
aag aac ggc gcg gat gca act tat gcc att gat cgt gat gcc gcg atc 403
Lys Asn Gly Ala Asp Ala Thr Tyr Ala Ile Asp Arg Asp Ala Ala Ile
90 95 100
gca tcc att gat ggt gca gct agc cta ggg gag aag gca cct cgc tac 451
Ala Ser Ile Asp Gly Ala Ala Ser Leu Gly Glu Lys Ala Pro Arg Tyr
105 110 115
atc atg gtg agc tac att gga tcc tcc acg cac acc att gat cct tca 499
Ile Met Val Ser Tyr Ile Gly Ser Ser Thr His Thr Ile Asp Pro Ser
120 125 130
gca tcc ttc tac cca tat gca gaa tcc aaa aag gcc gct gat gag cac 547
Ala Ser Phe Tyr Pro Tyr Ala Glu Ser Lys Lys Ala Ala Asp Glu His
135 140 145
cta agc tcc acc aac ctg gat tac ctt atc ctc gca cca gca gcc tta 595
Leu Ser Ser Thr Asn Leu Asp Tyr Leu Ile Leu Ala Pro Ala Ala Leu
150 155 160 165
act ctg gat gaa gtc aat ggc gtt gag gtg atc gcc gat acc tac gaa 643
Thr Leu Asp Glu Val Asn Gly Val Glu Val Ile Ala Asp Thr Tyr Glu
170 175 180
gca gcc gca ggc cgc acc aca tca aga gtc ctc gtt gcg gaa gtt atc 691
Ala Ala Ala Gly Arg Thr Thr Ser Arg Val Leu Val Ala Glu Val Ile
185 190 195
acc gag ttg gtg gtt cgc gac ttc cca caa acc cgt gtg ctg cct ttc 739
Thr Glu Leu Val Val Arg Asp Phe Pro Gln Thr Arg Val Leu Pro Phe
200 205 210
gtg gat ggc gaa tca cca gtc tcc tcg att agt taagctttgg gagttggtgc 792
Val Asp Gly Glu Ser Pro Val Ser Ser Ile Ser
215 220
tcagcaccat 802
<2l0>262
<211>224
<212>PRT
<213>谷氨酸棒状杆菌
<400>262
Val Ala Leu Met Thr Asn Lys Thr Arg Ala Leu Leu Ile Gly Gly His
1 5 10 15
Gly Lys Val Ala Leu Leu Ala Thr Pro Met Leu Ile Asp Ala Ser Val
20 25 30
Gln Val Thr Ser Met Tyr Arg Asn Pro Asp His Arg Ser Glu Ile Glu
35 40 45
Ala Leu Gly Ala Thr Thr Leu Glu Arg Asp Val Thr Thr Leu Ser Val
50 55 60
Glu Asp Trp Ala Asp Leu Leu Lys Asp Phe Asp Val Val Val Trp Ser
65 70 75 80
Ala Gly Asn Gly Gly Lys Asn Gly Ala Asp Ala Thr Tyr Ala Ile Asp
85 90 95
Arg Asp Ala Ala Ile Ala Ser Ile Asp Gly Ala Ala Ser Leu Gly Glu
100 105 110
Lys Ala Pro Arg Tyr Ile Met Val Ser Tyr Ile Gly Ser Ser Thr His
115 120 125
Thr Ile Asp Pro Ser Ala Ser Phe Tyr Pro Tyr Ala G1u Ser Lys Lys
130 135 140
Ala Ala Asp Glu His Leu Ser Ser Thr Asn Leu Asp Tyr Leu Ile Leu
145 150 155 160
Ala Pro Ala Ala Leu Thr Leu Asp Glu Val Asn Gly Val Glu Val Ile
165 170 175
Ala Asp Thr Tyr Glu Ala Ala Ala Gly Arg Thr Thr Ser Arg Val Leu
180 185 190
Val Ala Glu Val Ile Thr Glu Leu Val Val Arg Asp Phe Pro Gln Thr
195 200 205
Arg Val Leu Pro Phe Val Asp Gly Glu Ser Pro Val Ser Ser Ile Ser
210 215 220
<210>263
<211>1516
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1486)
<223>RXA02219
<400>263
tcaggaacgc atgaagccag tggaaaagga acagggcttg gttattcgta tccagtctga 60
agccttgttc aacgcggtta atgagatcta gggggtgcac atg gat gat ttt tct 115
Met Asp Asp Phe Ser
1 5
gca tcg cta ggt ttt act gac cgt tcc tcg agg gga att ctg gac acc 163
Ala Ser Leu Gly Phe Thr Asp Arg Ser Ser Arg Gly Ile Leu Asp Thr
10 15 20
atc gag atg att gag aag tat cgt ctt gat att cgc acc gtg acc atg 211
Ile Glu Met Ile Glu Lys Tyr Arg Leu Asp Ile Arg Thr Val Thr Met
25 30 35
gga atc agc ctg ttg gaa tgc gcg cgt ggc tcg atg gaa gag acc gcg 259
Gly Ile Ser Leu Leu Glu Cys Ala Arg Gly Ser Met Glu Glu Thr Ala
40 45 50
acg gct gtt tat gat cgc gtg acg tca cag gct gca cgt ctg gtt gag 307
Thr Ala Val Tyr Asp Arg Val Thr Ser Gln Ala Ala Arg Leu Val Glu
55 60 65
gtt tgt gaa ggc att gag cgt gaa ttg ggc att ccc att gtg aat aag 355
Val Cys Glu Gly Ile Glu Arg Glu Leu Gly Ile Pro Ile Val Asn Lys
70 75 80 85
cgc atc tcg gtg act cca att gcc ctg gtg act gct ggt tgc agt ggc 403
Arg Ile Ser Val Thr Pro Ile Ala Leu Val Thr Ala Gly Cys Ser Gly
90 95 100
gat ccc gtt gac gtt gct cga gcc tta gat aag gca gca aaa gat gtc 451
Asp Pro Val Asp Val Ala Arg Ala Leu Asp Lys Ala Ala Lys Asp Val
105 110 115
gga gtg aac ttc atc ggc ggg tat tca gca ctg gtt gaa aaa ggt gga 499
Gly Val Asn Phe Ile Gly Gly Tyr Ser Ala Leu Val Glu Lys Gly Gly
120 125 130
acc acc tcc gat att cgg ctg atc cgc tcc att ccc gag gca ttg agc 547
Thr Thr Ser Asp Ile Arg Leu Ile Arg Ser Ile Pro Glu Ala Leu Ser
135 140 145
aca acc gat gtg gtg tgt gga tcc gtc aat gtg gcg tcg tct cgc gcg 595
Thr Thr Asp Val Val Cys Gly Ser Val Asn Val Ala Ser Ser Arg Ala
150 155 160 165
ggt atc aac atg aac gca gtc aat gag atg ggc aag gtg gtc aag caa 643
Gly Ile Asn Met Asn Ala Val Asn Glu Met Gly Lys Val Val Lys Gln
170 175 180
gca gct gaa ttg acc aaa gat cgc tcc gcc atc gca tgt gca aag cta 691
Ala Ala Glu Leu Thr Lys Asp Arg Ser Ala Ile Ala Cys Ala Lys Leu
185 190 195
gtg gtc ttt gcc aat tct gtg ggc gat aat cca ttc atg gct ggc gct 739
Val Val Phe Ala Asn Ser Val Gly Asp Asn Pro Phe Met Ala Gly Ala
200 205 210
ttc cac ggc att gag gag ccg gat tgt gtg gtc agc gtt ggt gtc tca 787
Phe His Gly Ile Glu Glu Pro Asp Cys Val Val Ser Val Gly Val Ser
215 220 225
gga cca ggc gtg gtt agt cgc gcg ctg ggc aat ctt caa ggt gcc acc 835
Gly Pro Gly Val Val Ser Arg Ala Leu Gly Asn Leu Gln Gly Ala Thr
230 235 240 245
ctt gat caa gtc gca gaa gag atc aag aaa gcg gcc ttc aag atc act 883
Leu Asp Gln Val Ala Glu Glu Ile Lys Lys Ala Ala Phe Lys Ile Thr
250 255 260
cgt act gga caa ctg gtt ggt gcg atg gcc tca gaa cgc cta gga gtt 931
Arg Thr Gly Gln Leu Val Gly Ala Met Ala Ser Glu Arg Leu Gly Val
265 270 275
cct ttc ggc atc gtt gat ctt tcg ctc gct ccc act gct gaa gtg ggc 979
Pro Phe Gly Ile Val Asp Leu Ser Leu Ala Pro Thr Ala Glu Val Gly
280 285 290
gat tcc gtg gca aat atc ctt gag gtc atg ggt ctt gac cag gtg ggc 1027
Asp Ser Val Ala Asn Ile Leu Glu Val Met Gly Leu Asp Gln Val Gly
295 300 305
aca cat ggc acc acc gcg gca ctc gca ctg ctc aac gac gcg gtg aaa 1075
Thr His Gly Thr Thr Ala Ala Leu Ala Leu Leu Asn Asp Ala Val Lys
310 315 320 325
aag ggt ggc atg atg gcc tgc tca cgt gtc ggc ggc ttg tcc gga tcc 1123
Lys Gly Gly Met Met Ala Cys Ser Arg Val Gly Gly Leu Ser Gly Ser
330 335 340
ttc att cca gtt tct gaa gat aaa ggc atg atc gat gcg gtt cgc aca 1171
Phe Ile Pro Val Ser Glu Asp Lys Gly Met Ile Asp Ala Val Arg Thr
345 350 355
gga gca att tct att gac aag ctc gaa gcg atg acc gcg atc tgc tca 1219
Gly Ala Ile Ser Ile Asp Lys Leu Glu Ala Met Thr Ala Ile Cys Ser
360 365 370
gtt ggt ttg gat atg att gcg atc cct ggc gac acc ccg gcg gaa act 1267
Val Gly Leu Asp Met Ile Ala Ile Pro Gly Asp Thr Pro Ala Glu Thr
375 380 385
att tcg ggc atg atc gcg gac gaa gca gcc atc ggt gtg atg aat cat 1315
Ile Ser Gly Met Ile Ala Asp Glu Ala Ala Ile Gly Val Met Asn His
390 395 400 405
aag act aca gct gtg cgt gtt att cct gtt cca gga act gtc cct ggc 1363
Lys Thr Thr Ala Val Arg Val Ile Pro Val Pro Gly Thr Val Pro Gly
410 415 420
gat gag gta gat ttc ggt ggc ttg ctg ggt tac gcg ccg gtc att cca 1411
Asp Glu Val Asp Phe Gly Gly Leu Leu Gly Tyr Ala Pro Val Ile Pro
425 430 435
gta aat acc gtg gga aat agt gag ttt att cac cgc ggc ggc ttc att 1459
Val Asn Thr Val Gly Asn Ser Glu Phe Ile His Arg Gly Gly Phe Ile
440 445 450
cca gca ccc gtt cac ggg ttt aga aac taggagatcc ggggccttta 1506
Pro Ala Pro Val His Gly Phe Arg Asn
455 460
cctgccattt 1516
<210>264
<211>462
<212>PRT
<213>谷氨酸棒状杆菌
<400>264
Met Asp Asp Phe Ser Ala Ser Leu Gly Phe Thr Asp Arg Ser Ser Arg
1 5 10 15
Gly Ile Leu Asp Thr Ile Glu Met Ile Glu Lys Tyr Arg Leu Asp Ile
20 25 30
Arg Thr Val Thr Met Gly Ile Ser Leu Leu Glu Cys Ala Arg Gly Ser
35 40 45
Met Glu Glu Thr Ala Thr Ala Val Tyr Asp Arg Val Thr Ser Gln Ala
50 55 60
Ala Arg Leu Val Glu Val Cys Glu Gly Ile Glu Arg Glu Leu Gly Ile
65 70 75 80
Pro Ile Val Asn Lys Arg Ile Ser Val Thr Pro Ile Ala Leu Val Thr
85 90 95
Ala Gly Cys Ser Gly Asp Pro Val Asp Val Ala Arg Ala Leu Asp Lys
100 105 110
Ala Ala Lys Asp Val Gly Val Asn Phe Ile Gly Gly Tyr Ser Ala Leu
115 120 125
Val Glu Lys Gly Gly Thr Thr Ser Asp Ile Arg Leu Ile Arg Ser Ile
130 135 140
Pro Glu Ala Leu Ser Thr Thr Asp Val Val Cys Gly Ser Val Asn Val
145 150 155 160
Ala Ser Ser Arg Ala Gly Ile Asn Met Asn Ala Val Asn Glu Met Gly
165 170 175
Lys Val Val Lys Gln Ala Ala Glu Leu Thr Lys Asp Arg Ser Ala Ile
180 185 190
Ala Cys Ala Lys Leu Val Val Phe Ala Asn Ser Val Gly Asp Asn Pro
195 200 205
Phe Met Ala Gly Ala Phe His Gly Ile Glu Glu Pro Asp Cys Val Val
210 215 220
Ser Val Gly Val Ser Gly Pro Gly Val Val Ser Arg Ala Leu Gly Asn
225 230 235 240
Leu Gln Gly Ala Thr Leu Asp Gln Val Ala Glu Glu Ile Lys Lys Ala
245 250 255
Ala Phe Lys Ile Thr Arg Thr Gly Gln Leu Val Gly Ala Met Ala Ser
260 265 270
Glu Arg Leu Gly Val Pro Phe Gly Ile Val Asp Leu Ser Leu Ala Pro
275 280 285
Thr Ala Glu Val Gly Asp Ser Val Ala Asn Ile Leu Glu Val Met Gly
290 295 300
Leu Asp Gln Val Gly Thr His Gly Thr Thr Ala Ala Leu Ala Leu Leu
305 310 315 320
Asn Asp Ala Val Lys Lys Gly Gly Met Met Ala Cys Ser Arg Val Gly
325 330 335
Gly Leu Ser Gly Ser Phe Ile Pro Val Ser Glu Asp Lys Gly Met Ile
340 345 350
Asp Ala Val Arg Thr Gly Ala Ile Ser Ile Asp Lys Leu Glu Ala Met
355 360 365
Thr Ala Ile Cys Ser Val Gly Leu Asp Met Ile Ala Ile Pro Gly Asp
370 375 380
Thr Pro Ala Glu Thr Ile Ser Gly Met Ile Ala Asp Glu Ala Ala Ile
385 390 395 400
Gly Val Met Asn His Lys Thr Thr Ala Val Arg Val Ile Pro Val Pro
405 410 415
Gly Thr Val Pro Gly Asp Glu Val Asp Phe Gly Gly Leu Leu Gly Tyr
420 425 430
Ala Pro Val Ile Pro Val Asn Thr Val Gly Asn Ser Glu Phe Ile His
435 440 445
Arg Gly Gly Phe Ile Pro Ala Pro Val His Gly Phe Arg Asn
450 455 460
<210>265
<211>1492
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1462)
<223>RXA02221
<400>265
ttcaatgata tttgctctga tttttggttc tcgtctaatt tcattatttt gtcacgccag 60
ccatcacgac ttcataccca ccaattgaaa gtaaagtcca atg ttt acc gca tcc 115
Met Phe Thr Ala Ser
1 5
att cct act tat gaa tta ggg tct ttc agc aag ggc tcc tac gtt gag 163
Ile Pro Thr Tyr Glu Leu Gly Ser Phe Ser Lys Gly Ser Tyr Val Glu
10 15 20
atc aaa gga act tac ttc tac cct gac acg cta aat act tta ttt aga 211
Ile Lys Gly Thr Tyr Phe Tyr Pro Asp Thr Leu Asn Thr Leu Phe Arg
25 30 35
aat tct ggg gca aaa gac aac gaa gag ttc ttt gta gac gta act ctt 259
Asn Ser Gly Ala Lys Asp Asn Glu Glu Phe Phe Val Asp Val Thr Leu
40 45 50
gtt cca gaa cca gat aat ccg cat agc act cga ggc cat gcc att tca 307
Val Pro Glu Pro Asp Asn Pro His Ser Thr Arg Gly His Ala Ile Ser
55 60 65
gtc cgc tgg aat gat ctc gta ata gga cat att gcc tcg gat atg act 355
Val Arg Trp Asn Asp Leu Val Ile Gly His Ile Ala Ser Asp Met Thr
70 75 80 85
gaa aaa ttc caa caa gtg cga cga gtt gca gct agt ggc tac gat gct 403
Glu Lys Phe Gln Gln Val Arg Arg Val Ala Ala Ser Gly Tyr Asp Ala
90 95 100
cga gtc tct gct cgg att tgg acg aat aca aac tat aag aat gaa cga 451
Arg Val Ser Ala Arg Ile Trp Thr Asn Thr Asn Tyr Lys Asn Glu Arg
105 110 115
gat ttt tgg gta tct gtt aag ctc ccc gat cca gat ttc ctt gtt cct 499
Asp Phe Trp Val Ser Val Lys Leu Pro Asp Pro Asp Phe Leu Val Pro
120 125 130
ctt aat gat cct ccc cat gac ggg ttt acc ctc ctc cct tat gga aca 547
Leu Asn Asp Pro Pro His Asp Gly Phe Thr Leu Leu Pro Tyr Gly Thr
135 140 145
gca atc caa gtc acc aag gaa tct gat cac atg gac gtg ctc tca gaa 595
Ala Ile Gln Val Thr Lys Glu Ser Asp His Met Asp Val Leu Ser Glu
150 155 160 165
ttc gtg cct cca tct gga caa ggt cag att ctc gta tcg cta cac atc 643
Phe Val Pro Pro Ser Gly Gln Gly Gln Ile Leu Val Ser Leu His Ile
170 175 180
att aac gca ggc gtg aga aaa gaa tgg gat ggc att gaa gtt cgg ctc 691
Ile Asn Ala Gly Val Arg Lys Glu Trp Asp Gly Ile Glu Val Arg Leu
185 190 195
gat aat caa cgg atc gga gag ctt aca aag gcc agt tca gaa aaa ttc 739
Asp Asn Gln Arg Ile Gly Glu Leu Thr Lys Ala Ser Ser Glu Lys Phe
200 205 210
gca ccc gta gtg cga cat ttc gat gac cta gga ctt agt aca cta tgt 787
Ala Pro Val Val Arg His Phe Asp Asp Leu Gly Leu Ser Thr Leu Cys
215 220 225
cgt gcg ctc atc aag ggg tca tca cta gct gct gaa gta act cta tat 835
Arg Ala Leu Ile Lys Gly Ser Ser Leu Ala Ala Glu Val Thr Leu Tyr
230 235 240 245
gga gct cgt gca cat gag ctt tcc gaa gaa gac tta gaa cct aaa agc 883
Gly Ala Arg Ala His Glu Leu Ser Glu Glu Asp Leu Glu Pro Lys Ser
250 255 260
tct tcc cca ttg ccc agg ttg gtt gaa tac cag tca aat ccg ttt aat 931
Ser Ser Pro Leu Pro Arg Leu Val Glu Tyr Gln Ser Asn Pro Phe Asn
265 270 275
tat caa gtc ccc aac agg tgg ccc ggc gag cag aat caa cgt gct cct 979
Tyr Gln Val Pro Asn Arg Trp Pro Gly Glu Gln Asn Gln Arg Ala Pro
280 285 290
aag tcg caa aag tct tct tca tca cct ttc gca gcg tat cgc ggg cca 1027
Lys Ser Gln Lys Ser Ser Ser Ser Pro Phe Ala Ala Tyr Arg Gly Pro
295 300 305
tcg caa cct agt aaa tca ctc aca ccg ttt gta gcg cag aca cct gcg 1075
Ser Gln Pro Ser Lys Ser Leu Thr Pro Phe Val Ala Gln Thr Pro Ala
310 315 320 325
cct cag cct agt gat cga ttt att gat tgg gat tca ttg ctt caa ccg 1123
Pro Gln Pro Ser Asp Arg Phe Ile Asp Trp Asp Ser Leu Leu Gln Pro
330 335 340
gac gga act cca aga gct acg cca ttc caa aga gga gta acc cgc ggg 1171
Asp Gly Thr Pro Arg Ala Thr Pro Phe Gln Arg Gly Val Thr Arg Gly
345 350 355
tta att ggc aag cat ttt tcg aat caa aaa tct cca aga att gac ttt 1219
Leu Ile Gly Lys His Phe Ser Asn Gln Lys Ser Pro Arg Ile Asp Phe
360 365 370
gca acg gtg ggg caa tgt gat gca att ctc cga act ttc gga gag ccg 1267
Ala Thr Val Gly Gln Cys Asp Ala Ile Leu Arg Thr Phe Gly Glu Pro
375 380 385
acc gac aag ctt tat aaa gac ggc agg act tct tgg cct ttg tgg tgg 1315
Thr Asp Lys Leu Tyr Lys Asp Gly Arg Thr Ser Trp Pro Leu Trp Trp
390 395 400 405
gcg ctc atg gcg ata gtc acc ctt ctc gtc atg ttg ctg aac ttt att 1363
Ala Leu Met Ala Ile Val Thr Leu Leu Val Met Leu Leu Asn Phe Ile
410 415 420
cca gga att ggt ccg atc ttc cca ctt atc gga ttg atc gta ctt ggt 1411
Pro Gly Ile Gly Pro Ile Phe Pro Leu Ile Gly Leu Ile Val Leu Gly
425 430 435
cat cac ttt tgg act cgc cga aaa ctt aac cca cca ttc ggt aga tca 1459
His His Phe Trp Thr Arg Arg Lys Leu Asn Pro Pro Phe Gly Arg Ser
440 445 450
aaa tagatattct catcaaatat cgctgatgca 1492
Lys
<210>266
<211>454
<212>PRT
<213>谷氨酸棒状杆菌
<400>266
Met Phe Thr Ala Ser Ile Pro Thr Tyr Glu Leu Gly Ser Phe Ser Lys
1 5 10 15
Gly Ser Tyr Val Glu Ile Lys Gly Thr Tyr Phe Tyr Pro Asp Thr Leu
20 25 30
Asn Thr Leu Phe Arg Asn Ser Gly Ala Lys Asp Asn Glu Glu Phe Phe
35 40 45
Val Asp Val Thr Leu Val Pro Glu Pro Asp Asn Pro His Ser Thr Arg
50 55 60
Gly His Ala Ile Ser Val Arg Trp Asn Asp Leu Val Ile Gly His Ile
65 70 75 80
Ala Ser Asp Met Thr Glu Lys Phe Gln Gln Val Arg Arg Val Ala Ala
85 90 95
Ser Gly Tyr Asp Ala Arg Val Ser Ala Arg Ile Trp Thr Asn Thr Asn
100 105 110
Tyr Lys Asn Glu Arg Asp Phe Trp Val Ser Val Lys Leu Pro Asp Pro
115 120 125
Asp Phe Leu Val Pro Leu Asn Asp Pro Pro His Asp Gly Phe Thr Leu
130 135 140
Leu Pro Tyr Gly Thr Ala Ile Gln Val Thr Lys Glu Ser Asp His Met
145 150 155 160
Asp Val Leu Ser Glu Phe Val Pro Pro Ser Gly Gln Gly Gln Ile Leu
165 170 175
Val Ser Leu His Ile Ile Asn Ala Gly Val Arg Lys Glu Trp Asp Gly
180 185 190
Ile Glu Val Arg Leu Asp Asn Gln Arg Ile Gly Glu Leu Thr Lys Ala
195 200 205
Ser Ser Glu Lys Phe Ala Pro Val Val Arg His Phe Asp Asp Leu Gly
210 215 220
Leu Ser Thr Leu Cys Arg Ala Leu Ile Lys Gly Ser Ser Leu Ala Ala
225 230 235 240
Glu Val Thr Leu Tyr Gly Ala Arg Ala His Glu Leu Ser Glu Glu Asp
245 250 255
Leu Glu Pro Lys Ser Ser Ser Pro Leu Pro Arg Leu Val Glu Tyr Gln
260 265 270
Ser Asn Pro Phe Asn Tyr Gln Val Pro Asn Arg Trp Pro Gly Glu Gln
275 280 285
Asn Gln Arg Ala Pro Lys Ser Gln Lys Ser Ser Ser Ser Pro Phe Ala
290 295 300
Ala Tyr Arg Gly Pro Ser Gln Pro Ser Lys Ser Leu Thr Pro Phe Val
305 310 315 320
Ala Gln Thr Pro Ala Pro Gln Pro Ser Asp Arg Phe Ile Asp Trp Asp
325 330 335
Ser Leu Leu Gln Pro Asp Gly Thr Pro Arg Ala Thr Pro Phe Gln Arg
340 345 350
Gly Val Thr Arg Gly Leu Ile Gly Lys His Phe Ser Asn Gln Lys Ser
355 360 365
Pro Arg Ile Asp Phe Ala Thr Val Gly Gln Cys Asp Ala Ile Leu Arg
370 375 380
Thr Phe Gly Glu Pro Thr Asp Lys Leu Tyr Lys Asp Gly Arg Thr Ser
385 390 395 400
Trp Pro Leu Trp Trp Ala Leu Met Ala Ile Val Thr Leu Leu Val Met
405 410 415
Leu Leu Asn Phe Ile Pro Gly Ile Gly Pro Ile Phe Pro Leu Ile Gly
420 425 430
Leu Ile Val Leu Gly His His Phe Trp Thr Arg Arg Lys Leu Asn Pro
435 440 445
Pro Phe Gly Arg Ser Lys
450
<210>267
<211>1003
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(973)
<223>RXA02267
<400>267
tgcgctcggc aagtgttttg cttatcgacg tctccccaca taacaatccc aactcgaagc 60
accaacgatt caagccttat cagttttgta caggaaaata gtg caa aaa tgg ggt 115
Val Gln Lys Trp Gly
1 5
tta agc ttc gtg gag agg att gtc atc atg aac aac gtg caa cag ttt 163
Leu Ser Phe Val Glu Arg Ile Val Ile Met Asn Asn Val Gln Gln Phe
10 15 20
cat cga ttt ttt gat gat tcc gca gtc tat tat ccc tgc ttc gtc ccg 211
His Arg Phe Phe Asp Asp Ser Ala Val Tyr Tyr Pro Cys Phe Val Pro
25 30 35
ctt gac cga gcc atc ggc gaa cac ttt gat cgt cag aac aaa ccg atg 259
Leu Asp Arg Ala Ile Gly Glu His Phe Asp Arg Gln Asn Lys Pro Met
40 45 50
tcc aga ttc atc gga acg ctc att ctg ccg tta gcc aaa ctg gaa gaa 307
Ser Arg Phe Ile Gly Thr Leu Ile Leu Pro Leu Ala Lys Leu Glu Glu
55 60 65
gcc gcc caa tac acc ggc gat gaa gtc ctt cgc gtg tcg gca gta atc 355
Ala Ala Gln Tyr Thr Gly Asp Glu Val Leu Arg Val Ser Ala Val Ile
70 75 80 85
agt act gat ggg ctc gct gat ctg cga agg gat ttt tac gaa ctc ccc 403
Ser Thr Asp Gly Leu Ala Asp Leu Arg Arg Asp Phe Tyr Glu Leu Pro
90 95 100
aac atc gac atc gcc tcg gtg gaa atc aag ctg gtc ggc gca gcc ctc 451
Asn Ile Asp Ile Ala Ser Val Glu Ile Lys Leu Val Gly Ala Ala Leu
105 110 115
acc aac acc gct tgg ttg gga gat gtg gaa aaa ctc atc caa caa cat 499
Thr Asn Thr Ala Trp Leu Gly Asp Val Glu Lys Leu Ile Gln Gln His
120 125 130
cgc aac act ttc gta tgg gtt gag att ccg aca gcc ctg gtc acc gca 547
Arg Asn Thr Phe Val Trp Val Glu Ile Pro Thr Ala Leu Val Thr Ala
135 140 145
gat att gtc cga aaa ctc cgc cac atg gga gct ggc ctg aaa tac aga 595
Asp Ile Val Arg Lys Leu Arg His Met Gly Ala Gly Leu Lys Tyr Arg
150 155 160 165
act gga ggt gat agg gaa gag ctc ttc ccc tca ccg cag gac ttg gtc 643
Thr Gly Gly Asp Arg Glu Glu Leu Phe Pro Ser Pro Gln Asp Leu Val
170 175 180
act gtg ctg cgc acc gcc atc gat gct gca ttg ccg ttt aaa ctc act 691
Thr Val Leu Arg Thr Ala Ile Asp Ala Ala Leu Pro Phe Lys Leu Thr
185 190 195
gca ggc ctg cat cgt gct ctc agg tat cgt gac gag aaa acc ggc cga 739
Ala Gly Leu His Arg Ala Leu Arg Tyr Arg Asp Glu Lys Thr Gly Arg
200 205 210
ctt cac ttc gga ttc ctc aac att gca gcc gcc gtg gcg aca ctt cgt 787
Leu His Phe Gly Phe Leu Asn Ile Ala Ala Ala Val Ala Thr Leu Arg
215 220 225
gct gga aaa ggc gag gca gag gca ctg aag atc ctt gaa ggc gat gat 835
Ala Gly Lys Gly Glu Ala Glu Ala Leu Lys Ile Leu Glu Gly Asp Asp
230 235 240 245
gcc gct ccg ctt att cac gca cta caa agc ggc gaa aac tgg cgg gat 883
Ala Ala Pro Leu Ile His Ala Leu Gln Ser Gly Glu Asn Trp Arg Asp
250 255 260
tcc ttc cgc agc ttc agt acc tgc aat gtt gtt gaa cca ctc aac act 931
Ser Phe Arg Ser Phe Ser Thr Cys Asn Val Val Glu Pro Leu Asn Thr
265 270 275
ctg att gat ctt gat gtg ttg gcg gaa gga gac gta cat ccc 973
Leu Ile Asp Leu Asp Val Leu Ala Glu Gly Asp Val His Pro
280 285 290
taaggatcga cgctagttag atcggttttt 1003
<210>268
<211>291
<212>PRT
<213>谷氨酸棒状杆菌
<400>268
Val Gln Lys Trp Gly Leu Ser Phe Val Glu Arg Ile Val Ile Met Asn
1 5 10 15
Asn Val Gln Gln Phe His Arg Phe Phe Asp Asp Ser Ala Val Tyr Tyr
20 25 30
Pro Cys Phe Val Pro Leu Asp Arg Ala Ile Gly Glu His Phe Asp Arg
35 40 45
Gln Asn Lys Pro Met Ser Arg Phe Ile Gly Thr Leu Ile Leu Pro Leu
50 55 60
Ala Lys Leu Glu Glu Ala Ala Gln Tyr Thr Gly Asp Glu Val Leu Arg
65 70 75 80
Val Ser Ala Val Ile Ser Thr Asp Gly Leu Ala Asp Leu Arg Arg Asp
85 90 95
Phe Tyr Glu Leu Pro Asn Ile Asp Ile Ala Ser Val Glu Ile Lys Leu
100 105 110
Val Gly Ala Ala Leu Thr Asn Thr Ala Trp Leu Gly Asp Val Glu Lys
115 120 125
Leu Ile Gln Gln His Arg Asn Thr Phe Val Trp Val Glu Ile Pro Thr
130 135 140
Ala Leu Val Thr Ala Asp Ile Val Arg Lys Leu Arg His Met Gly Ala
145 150 155 160
Gly Leu Lys Tyr Arg Thr Gly Gly Asp Arg Glu Glu Leu Phe Pro Ser
165 170 175
Pro Gln Asp Leu Val Thr Val Leu Arg Thr Ala Ile Asp Ala Ala Leu
180 185 190
Pro Phe Lys Leu Thr Ala Gly Leu His Arg Ala Leu Arg Tyr Arg Asp
195 200 205
Glu Lys Thr Gly Arg Leu His Phe Gly Phe Leu Asn Ile Ala Ala Ala
210 215 220
Val Ala Thr Leu Arg Ala Gly Lys Gly Glu Ala Glu Ala Leu Lys Ile
225 230 235 240
Leu Glu Gly Asp Asp Ala Ala Pro Leu Ile His Ala Leu Gln Ser Gly
245 250 255
Glu Asn Trp Arg Asp Ser Phe Arg Ser Phe Ser Thr Cys Asn Val Val
260 265 270
Glu Pro Leu Asn Thr Leu Ile Asp Leu Asp Val Leu Ala Glu Gly Asp
275 280 285
Val His Pro
290
<210>269
<211>688
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(658)
<223>RXA02271
<400>269
cactccccta tcctaagcca catctcggtt tattaaactg ttagtgaatt ctcacccgca 60
acactggtgt atttcagtta catccgtatg gttattggtt atg agc ttt ctt aac 115
Met Ser Phe Leu Asn
1 5
tct gca aaa acc aag acc gta gcc ctc acc gca acc ttc gtt ggt gca 163
Ser Ala Lys Thr Lys Thr Val Ala Leu Thr Ala Thr Phe Val Gly Ala
10 15 20
gca acc ctt gca act cct gca atc gca tcc gct gac atc gtc gac aac 211
Ala Thr Leu Ala Thr Pro Ala Ile Ala Ser Ala Asp Ile Val Asp Asn
25 30 35
gcc ctc gca gcc ctc cca tcc ggt gag atc agc tgc tcc cag gct gaa 259
Ala Leu Ala Ala Leu Pro Ser Gly Glu Ile Ser Cys Ser Gln Ala Glu
40 45 50
aag tac tgg acc acc gaa gct gat tac aac agc aag gtt gca cag gcc 307
Lys Tyr Trp Thr Thr Glu Ala Asp Tyr Asn Ser Lys Val Ala Gln Ala
55 60 65
aac gcc ttg gca atg ttt gac tcc cgc ggc cca cag atc cag gca gct 355
Asn Ala Leu Ala Met Phe Asp Ser Arg Gly Pro Gln Ile Gln Ala Ala
70 75 80 85
ctc gca cgc gtt gac gaa gca gca aac cgc tgc gga ctc aag ggc ggc 403
Leu Ala Arg Val Asp Glu Ala Ala Asn Arg Cys Gly Leu Lys Gly Gly
90 95 100
acc gta gct gcg cag gct gag gca act gag gct gcg cct gcc gct cca 451
Thr Val Ala Ala Gln Ala Glu Ala Thr Glu Ala Ala Pro Ala Ala Pro
105 110 115
gca cct gca ccg cag gat aac acc ggc act tct cag act gcc cct gcc 499
Ala Pro Ala Pro Gln Asp Asn Thr Gly Thr Ser Gln Thr Ala Pro Ala
120 125 130
cca gca gca cca gca gca cca gca gct acc cct gtg gtt aac ctt gca 547
Pro Ala Ala Pro Ala Ala Pro Ala Ala Thr Pro Val Val Asn Leu Ala
135 140 145
cct gca gga tca cca act ttc acc att gaa gtt cca gga gtt ggc ggg 595
Pro Ala Gly Ser Pro Thr Phe Thr Ile Glu Val Pro Gly Val Gly Gly
150 155 160 165
gtt cag ctg cca gat cta tac caa atc gtc caa cag ttc ttg gca cag 643
Val Gln Leu Pro Asp Leu Tyr Gln Ile Val Gln Gln Phe Leu Ala Gln
170 175 180
ttc gga atc aag atc taaatctatt cacatccctt aaccctcacg 688
Phe Gly Ile Lys Ile
185
<2l0>270
<211>186
<212>PRT
<213>谷氨酸棒状杆菌
<400>270
Met Ser Phe Leu Asn Ser Ala Lys Thr Lys Thr Val Ala Leu Thr Ala
1 5 10 15
Thr Phe Val Gly Ala Ala Thr Leu Ala Thr Pro Ala Ile Ala Ser Ala
20 25 30
Asp Ile Val Asp Asn Ala Leu Ala Ala Leu Pro Ser Gly Glu Ile Ser
35 40 45
Cys Ser Gln Ala Glu Lys Tyr Trp Thr Thr Glu Ala Asp Tyr Asn Ser
50 55 60
Lys Val Ala Gln Ala Asn Ala Leu Ala Met Phe Asp Ser Arg Gly Pro
65 70 75 80
Gln Ile Gln Ala Ala Leu Ala Arg Val Asp Glu Ala Ala Asn Arg Cys
85 90 95
Gly Leu Lys Gly Gly Thr Val Ala Ala Gln Ala Glu Ala Thr Glu Ala
100 105 110
Ala Pro Ala Ala Pro Ala Pro Ala Pro Gln Asp Asn Thr Gly Thr Ser
115 120 125
Gln Thr Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Ala Thr Pro
130 135 140
Val Val Asn Leu Ala Pro Ala Gly Ser Pro Thr Phe Thr Ile Glu Val
145 150 155 160
Pro Gly Val Gly Gly Val Gln Leu Pro Asp Leu Tyr Gln Ile Val Gln
165 170 175
Gln Phe Leu Ala Gln Phe Gly Ile Lys Ile
180 185
<210>271
<211>1984
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1954)
<223>RXA02280
<400>271
ggtcgaggtg tcgtagatgt caatgagctt cgcgattgcg tcatcgatcg ttgttgcttc 60
catgcgcacc acactatctt tctgcacgcc ctgatgccct gtg gat tca aaa ctg 115
Val Asp Ser Lys Leu
1 5
tgc ttt tat agg cgt atg caa gaa tcc tca cgt gat aat ttc caa gtt 163
Cys Phe Tyr Arg Arg Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val
10 15 20
gac ctc ggc ggc gtt gtt gat ctt ttg agt cgc cac att tat tcc ggt 211
Asp Leu Gly Gly Val Val Asp Leu Leu Ser Arg His Ile Tyr Ser Gly
25 30 35
ccg agg gtg tat gtg cgt gag ttg ctg cag aat gcg gtt gat gct tgt 259
Pro Arg Val Tyr Val Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys
40 45 50
act gca cgt tct gaa cag ggt gag gag ggc tac gag ccg agt att cgt 307
Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly Tyr Glu Pro Ser Ile Arg
55 60 65
att cgg ccg gtg acc aag gat cgt gcc acg ttt tca ctg gtt gat aat 355
Ile Arg Pro Val Thr Lys Asp Arg Ala Thr Phe Ser Leu Val Asp Asn
70 75 80 85
ggt acg ggc ctg acc gcg cag gag gcg cgg gaa ttg ctg gcg acg gtg 403
Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg Glu Leu Leu Ala Thr Val
90 95 100
ggg cgg acg tcg aaa cgc gat gaa ttc ggt ctg cag cgg gaa ggt cgc 451
Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg
105 110 115
ctg ggg caa ttt ggc atc ggg ctg ctt agt tgt ttc atg gtg gcg gat 499
Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser Cys Phe Met Val Ala Asp
120 125 130
gag atc acc atg gtg tcg cat gcg gag ggt gcg tcg gcg att cgg tgg 547
Glu Ile Thr Met Val Ser His Ala Glu Gly Ala Ser Ala Ile Arg Trp
135 140 145
act ggt cat gcg gat ggc acc ttt aac ctg gag att ctt ggg gat gac 595
Thr Gly His Ala Asp Gly Thr Phe Asn Leu Glu Ile Leu Gly Asp Asp
150 155 160 165
gca acg gat gtc att ccg gtg ggc acg act gtg cac ctg act ccg cgc 643
Ala Thr Asp Val Ile Pro Val Gly Thr Thr Val His Leu Thr Pro Arg
170 175 180
cct gat gag cgc acg ttg ctg acg gaa aat tcc gtg gtc acc att gct 691
Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn Ser Val Val Thr Ile Ala
185 190 195
agt aat tat ggc cgc tac ctg ccg att cct att gtg gtg cag ggt gag 739
Ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro Ile Val Val Gln Gly Glu
200 205 210
aaa aac acc acc atc act aca tcg ccg gtg ttt gca aag gat act gat 787
Lys Asn Thr Thr Ile Thr Thr Ser Pro Val Phe Ala Lys Asp Thr Asp
215 220 225
cag cag cac agg ctg tat gcc ggc cgg gag cgc ctt ggt aaa act cct 835
Gln Gln His Arg Leu Tyr Ala Gly Arg Glu Arg Leu Gly Lys Thr Pro
230 235 240 245
ttt gat gtc atc gat ctc acc ggt cct ggc atc gag ggt gtg gct tat 883
Phe Asp Val Ile Asp Leu Thr Gly Pro Gly Ile Glu Gly Val Ala Tyr
250 255 260
gta ttg ccg gag gcc cag gct ccg cat atg tcc agg cgt cac agt att 931
Val Leu Pro Glu Ala Gln Ala Pro His Met Ser Arg Arg His Ser Ile
265 270 275
tat gtc aac cgc atg ttg gtc tct gat ggg cct tcc acg gtg ctg ccc 979
Tyr Val Asn Arg Met Leu Val Ser Asp Gly Pro Ser Thr Val Leu Pro
280 285 290
aac tgg gcg ttc ttt gtg gaa tgt gaa atc aat tca acc gat ttg gaa 1027
Asn Trp Ala Phe Phe Val Glu Cys Glu Ile Asn Ser Thr Asp Leu Glu
295 300 305
ccc acc gca tcg cgt gaa gcg ctc atg gat gac acc gcg ttc gcg gca 1075
Pro Thr Ala Ser Arg Glu Ala Leu Met Asp Asp Thr Ala Phe Ala Ala
310 315 320 325
acc agg gaa cat atc ggt gag tgc att aaa tcg tgg ctg att aat ctc 1123
Thr Arg Glu His Ile Gly Glu Cys Ile Lys Ser Trp Leu Ile Asn Leu
330 335 340
gcc atg acc aag cct cac cgc gtg cgg gaa ttt act gcg att cat gat 1171
Ala Met Thr Lys Pro His Arg Val Arg Glu Phe Thr Ala Ile His Asp
345 350 355
ctt gcc ctg cgc gag ctg tgc caa tcg gac gcg gac ctg gct gaa acc 1219
Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp Ala Asp Leu Ala Glu Thr
360 365 370
atg ttg ggt ctt ctc acc ttg gag acc tcc cgt ggt cgc atc tcg atc 1267
Met Leu Gly Leu Leu Thr Leu Glu Thr Ser Arg Gly Arg Ile Ser Ile
375 380 385
ggt gag atc acc acg ttg tcc atc acc gag gat gtg tcg ctg cag ctg 1315
Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu Asp Val Ser Leu Gln Leu
390 395 400 405
gct acc acg ttg gat gat ttc agg cag ctc aac acc att gcg cgc ccg 1363
Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu Asn Thr Ile Ala Arg Pro
410 415 420
gac acc ttg att att aat ggc ggc tac att cac gac agc gat ctg gct 1411
Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile His Asp Ser Asp Leu Ala
425 430 435
cgg ctc att ccc gtt cac tac cca ccg ctt acg gta tct act gct gac 1459
Arg Leu Ile Pro Val His Tyr Pro Pro Leu Thr Val Ser Thr Ala Asp
440 445 450
ctg cgc gaa tcc atg gat ctg atg gag ctt ccg ccg ctg cag gac att 1507
Leu Arg Glu Ser Met Asp Leu Met Glu Leu Pro Pro Leu Gln Asp Ile
455 460 465
gag aaa gcc aag gca ctg gat gcg cag gtc acg gaa tca ttg aag gat 1555
Glu Lys Ala Lys Ala Leu Asp Ala Gln Val Thr Glu Ser Leu Lys Asp
470 475 480 485
ttt cag atc aag ggc gca acg agg gtt ttt gaa ccc gca gat gtt cct 1603
Phe Gln Ile Lys Gly Ala Thr Arg Val Phe Glu Pro Ala Asp Val Pro
490 495 500
gcc gtg gtg atc att gat tcc aag gcg cag gcc tca cgg gat cgc aat 1651
Ala Val Val Ile Ile Asp Ser Lys Ala Gln Ala Ser Arg Asp Arg Asn
505 510 515
gaa aca caa agc gca acc act gat cgt tgg gct gac att ttg gca acg 1699
Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp Ala Asp Ile Leu Ala Thr
520 525 530
gtg gat aac acg ttg agc cgt caa aca gcc aac att cca cag gat cag 1747
Val Asp Asn Thr Leu Ser Arg Gln Thr Ala Asn Ile Pro Gln Asp Gln
535 540 545
gga ctg tcg gcg ttg tgc ttg aat tgg aac aat tcg ctg gtc agg aaa 1795
Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn Asn Ser Leu Val Arg Lys
550 555 560 565
ttg gcg tcc act gat gac acc gcc gtg gtg tcg cgc acg gtg cgt ttg 1843
Leu Ala Ser Thr Asp Asp Thr Ala Val Val Ser Arg Thr Val Arg Leu
570 575 580
ctc tac gtt cag gca ttg ttg tcc agc aag agg cca ctg cgg gtg aag 1891
Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys Arg Pro Leu Arg Val Lys
585 590 595
gaa cgc gcg ctg ctt aat gat tcg ctg gca gat ctg gtt tct ttg tct 1939
Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala Asp Leu Val Ser Leu Ser
600 605 610
ttg tca tcc gat atc taagacaatc ctccgctaat ctcgagggca 1984
Leu Ser Ser Asp Ile
615
<210>272
<211>618
<212>PRT
<213>谷氨酸棒状杆菌
<400>272
Val Asp Ser Lys Leu Cys Phe Tyr Arg Arg Met Gln Glu Ser Ser Arg
1 5 10 15
Asp Asn Phe Gln Val Asp Leu Gly Gly Val Val Asp Leu Leu Ser Arg
20 25 30
His Ile Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln Asn
35 40 45
Ala Val Asp Ala Cys Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly Tyr
50 55 60
Glu Pro Ser Ile Arg Ile Arg Pro Val Thr Lys Asp Arg Ala Thr Phe
65 70 75 80
Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg Glu
85 90 95
Leu Leu Ala Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly Leu
100 105 110
Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser Cys
115 120 125
Phe Met Val Ala Asp Glu Ile Thr Met Val Ser His Ala Glu Gly Ala
130 135 140
Ser Ala Ile Arg Trp Thr Gly His Ala Asp Gly Thr Phe Asn Leu Glu
145 150 155 160
Ile Leu Gly Asp Asp Ala Thr Asp Val Ile Pro Val Gly Thr Thr Val
165 170 175
His Leu Thr Pro Arg Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn Ser
180 185 190
Val Val Thr Ile Ala Ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro Ile
195 200 205
Val Val Gln Gly Glu Lys Asn Thr Thr Ile Thr Thr Ser Pro Val Phe
210 215 220
Ala Lys Asp Thr Asp Gln Gln His Arg Leu Tyr Ala Gly Arg Glu Arg
225 230 235 240
Leu Gly Lys Thr Pro Phe Asp Val Ile Asp Leu Thr Gly Pro Gly Ile
245 250 255
Glu Gly Val Ala Tyr Val Leu Pro Glu Ala Gln Ala Pro His Met Ser
260 265 270
Arg Arg His Ser Ile Tyr Val Asn Arg Met Leu Val Ser Asp Gly Pro
275 280 285
Ser Thr Val Leu Pro Asn Trp Ala Phe Phe Val Glu Cys Glu Ile Asn
290 295 300
Ser Thr Asp Leu Glu Pro Thr Ala Ser Arg Glu Ala Leu Met Asp Asp
305 310 315 320
Thr Ala Phe Ala Ala Thr Arg Glu His Ile Gly Glu Cys Ile Lys Ser
325 330 335
Trp Leu Ile Asn Leu Ala Met Thr Lys Pro His Arg Val Arg Glu Phe
340 345 350
Thr Ala Ile His Asp Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp Ala
355 360 365
Asp Leu Ala Glu Thr Met Leu Gly Leu Leu Thr Leu Glu Thr Ser Arg
370 375 380
Gly Arg Ile Ser Ile Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu Asp
385 390 395 400
Val Ser Leu Gln Leu Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu Asn
405 410 415
Thr Ile Ala Arg Pro Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile His
420 425 430
Asp Ser Asp Leu Ala Arg Leu Ile Pro Val His Tyr Pro Pro Leu Thr
435 440 445
Val Ser Thr Ala Asp Leu Arg Glu Ser Met Asp Leu Met Glu Leu Pro
450 455 460
Pro Leu Gln Asp Ile Glu Lys Ala Lys Ala Leu Asp Ala Gln Val Thr
465 470 475 480
Glu Ser Leu Lys Asp Phe Gln Ile Lys Gly Ala Thr Arg Val Phe Glu
485 490 495
Pro Ala Asp Val Pro Ala Val Val Ile Ile Asp Ser Lys Ala Gln Ala
500 505 510
Ser Arg Asp Arg Asn Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp Ala
515 520 525
Asp Ile Leu Ala Thr Val Asp Asn Thr Leu Ser Arg Gln Thr Ala Asn
530 535 540
Ile Pro Gln Asp Gln Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn Asn
545 550 555 560
Ser Leu Val Arg Lys Leu Ala Ser Thr Asp Asp Thr Ala Val Val Ser
565 570 575
Arg Thr Val Arg Leu Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys Arg
580 585 590
Pro Leu Arg Val Lys Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala Asp
595 600 605
Leu Val Ser Leu Ser Leu Ser Ser Asp Ile
610 615
<210>273
<211>514
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(484)
<223>RXA02294
<400>273
atgagcgtct acctcacctg gcaacaaatc caaggaatgt agataagtcg gggcaaactc 60
taagaaatca tcctggtttg tggcagggaa cattcctagg atg gga ggc atg gca 115
Met Gly Gly Met Ala
1 5
aag aca aaa cta gaa agc atc tcc gag cgc aac tcg gat gat ccg ctt 163
Lys Thr Lys Leu Glu Ser Ile Ser Glu Arg Asn Ser Asp Asp Pro Leu
10 15 20
agt tac ccc gac ctg act ctt gca cca ata tcc aca aca gtc aca gtc 211
Ser TVr Pro Asp Leu Thr Leu Ala Pro Ile Ser Thr Thr Val Thr Val
25 30 35
gtg gtc gcc gaa gac acc cca aac gat aac ttc tca gaa ctt ctc ctc 259
Val Val Ala Glu Asp Thr Pro Asn Asp Asn Phe Ser Glu Leu Leu Leu
40 45 50
ggc gca gca gaa tca acc cgt ctt tta gga ctc gac gag ctc cac atc 307
Gly Ala Ala Glu Ser Thr Arg Leu Leu Gly Leu Asp Glu Leu His Ile
55 60 65
atc gct cca tca gtc cac cta ccc gct ctc gca gta gca gcc gcc gac 355
Ile Ala Pro Ser Val His Leu Pro Ala Leu Ala Val Ala Ala Ala Asp
70 75 80 85
atc gca cac cat ctg cca gag aaa ttc caa ttc tgt gaa gcc gaa acc 403
Ile Ala His His Leu Pro Glu Lys Phe Gln Phe Cys Glu Ala Glu Thr
90 95 100
tgc act cat ctc cac ccc gac gat gac acc tat ctc acg gct gaa tcc 451
Cys Thr His Leu His Pro Asp Asp Asp Thr Tyr Leu Thr Ala Glu Ser
105 110 115
gtt gct caa ttg ggt acg aag ctg aag tct gcg tagttgttag gagccaccac 504
Val Ala Gln Leu Gly Thr Lys Leu Lys Ser Ala
120 125
agacccgtag 514
<210>274
<211>128
<212>PRT
<213>谷氨酸棒状杆菌
<400>274
Met Gly Gly Met Ala Lys Thr Lys Leu Glu Ser Ile Ser Glu Arg Asn
1 5 10 15
Ser Asp Asp Pro Leu Ser Tyr Pro Asp Leu Thr Leu Ala Pro Ile Ser
20 25 30
Thr Thr Val Thr Val Val Val Ala Glu Asp Thr Pro Asn Asp Asn Phe
35 40 45
Ser Glu Leu Leu Leu Gly Ala Ala Glu Ser Thr Arg Leu Leu Gly Leu
50 55 60
Asp Glu Leu His Ile Ile Ala Pro Ser Val His Leu Pro Ala Leu Ala
65 70 75 80
Val Ala Ala Ala Asp Ile Ala His His Leu Pro Glu Lys Phe Gln Phe
85 90 95
Cys Glu Ala Glu Thr Cys Thr His Leu His Pro Asp Asp Asp Thr Tyr
100 105 110
Leu Thr Ala Glu Ser Val Ala Gln Leu Gly Thr Lys Leu Lys Ser Ala
115 120 125
<210>275
<211>910
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(880)
<223>RXA02295
<400>275
ttcccatgct aatccgcgaa aatggcactt caacagccat atactttaaa caacacaaat 60
gatgcaagac tgataccggg atgtgatagg agcgcaccac atg ggg ttg gaa tta 115
Met Gly Leu Glu Leu
1 5
gca gct agc ggg tgg ggc atc ctc atc gca ggc gcc gcc gta gcc gga 163
Ala Ala Ser Gly Trp Gly Ile Leu Ile Ala Gly Ala Ala Val Ala Gly
10 15 20
tgg atc gac gca gta atc ggc ggt ggc gga ctc gtc ctc atc ccg ctg 211
Trp Ile Asp Ala Val Ile Gly Gly Gly Gly Leu Val Leu Ile Pro Leu
25 30 35
atc ctc gcg gtc atg ccg caa ctc gca cct gtg aca gcg ctg gcc tcc 259
Ile Leu Ala Val Met Pro Gln Leu Ala Pro Val Thr Ala Leu Ala Ser
40 45 50
aac aaa ctg gca gcc gtc acc ggc acg gca tcg gcg gca ttc acc ctg 307
Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser Ala Ala Phe Thr Leu
55 60 65
gtc agg cgc gtc aaa ccc gac aaa aaa ctg ctt gcg ctc tac gtt ctg 355
Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu Ala Leu Tyr Val Leu
70 75 80 85
gtg gca gct gtg tgc tcc ggt gca ggc gcc ctg gct gcg agt ctc att 403
Val Ala Ala Val Cys Ser Gly Ala Gly Ala Leu Ala Ala Ser Leu Ile
90 95 100
gac aaa caa atc atg cga ccg ctg atc atc gtg ttg atg ctg gtc gtt 451
Asp Lys Gln Ile Met Arg Pro Leu Ile Ile Val Leu Met Leu Val Val
105 110 115
ggc ctg atc gtg gtg ttc aaa cca aac ttc gga acc ggc gaa agc aaa 499
Gly Leu Ile Val Val Phe Lys Pro Asn Phe Gly Thr Gly Glu Ser Lys
120 125 130
gcc ctg ccc acc gga tgg aaa cgc tgg gcc gcc atc gtt gca gtc gga 547
Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala Ile Val Ala Val Gly
135 140 145
ctc atc gca gcc tac gac ggc atc ttc gga ccc gga acc ggc atg ttc 595
Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro Gly Thr Gly Met Phe
150 155 160 165
ctc atc atg gcg ttc acc gca ctg ctc tcc caa aat ttc ctg tcc tcc 643
Leu Ile Met Ala Phe Thr Ala Leu Leu Ser Gln Asn Phe Leu Ser Ser
170 175 180
gca gcc atg gcg aag gtc gta aac acc gca aca aac ctg ggt gcg cta 691
Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr Asn Leu Gly Ala Leu
185 190 195
att gta ttc atc atc ggc ggc cac atg tgg tgg acc cta gga ctc gtg 739
Ile Val Phe Ile Ile Gly Gly His Met Trp Trp Thr Leu Gly Leu Val
200 205 210
ctg gca gtc gcc aat gtc gca ggc gca caa ctc ggt gcc cga acg gtg 787
Leu Ala Val Ala Asn Val Ala Gly Ala Gln Leu Gly Ala Arg Thr Val
215 220 225
ctt ggt ggc ggt acc agg cta att aga tac gca cta cta acc ctg gtt 835
Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala Leu Leu Thr Leu Val
230 235 240 245
gtc gtc atg agc gtc tac ctc acc tgg caa caa atc caa gga atg 880
Val Val Met Ser Val Tyr Leu Thr Trp Gln Gln Ile Gln Gly Met
250 255 260
tagataagtc ggggcaaact ctaagaaatc 910
<210>276
<211>260
<212>PRT
<213>谷氨酸棒状杆菌
<400>276
Met Gly Leu Glu Leu Ala Ala Ser Gly Trp Gly Ile Leu Ile Ala Gly
1 5 10 15
Ala Ala Val Ala Gly Trp Ile Asp Ala Val Ile Gly Gly Gly Gly Leu
20 25 30
Val Leu Ile Pro Leu Ile Leu Ala Val Met Pro Gln Leu Ala Pro Val
35 40 45
Thr Ala Leu Ala Ser Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser
50 55 60
Ala Ala Phe Thr Leu Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu
65 70 75 80
Ala Leu Tyr Val Leu Val Ala Ala Val Cys Ser Gly Ala Gly Ala Leu
85 90 95
Ala Ala Ser Leu Ile Asp Lys Gln Ile Met Arg Pro Leu Ile Ile Val
100 105 110
Leu Met Leu Val Val Gly Leu Ile Val Val Phe Lys Pro Asn Phe Gly
115 120 125
Thr Gly Glu Ser Lys Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala
130 135 140
Ile Val Ala Val Gly Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro
145 150 155 160
Gly Thr Gly Met Phe Leu Ile Met Ala Phe Thr A1a Leu Leu Ser Gln
165 170 175
Asn Phe Leu Ser Ser Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr
180 185 190
Asn Leu Gly Ala Leu Ile Val Phe Ile Ile Gly Gly His Met Trp Trp
195 200 205
Thr Leu Gly Leu Val Leu Ala Val Ala Asn Val Ala Gly Ala Gln Leu
210 215 220
Gly Ala Arg Thr Val Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala
225 230 235 240
Leu Leu Thr Leu Val Val Val Met Ser Val Tyr Leu Thr Trp Gln Gln
245 250 255
Ile Gln Gly Met
260
<210>277
<211>619
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(589)
<223>RXA02296
<400>277
gcatcatttg tgttgtttaa agtatatggc tgttgaagtg ccattttcgc ggattagcat 60
gggaatcacc agtatttctg gacggttaag gatgattcat atg cgt aat caa aca 115
Met Arg Asn Gln Thr
1 5
atc gct gcg gtc gca gct ttg gtc ctg ctc acc gcc gcc acg ccc gcg 163
Ile Ala Ala Val Ala Ala Leu Val Leu Leu Thr Ala Ala Thr Pro Ala
10 15 20
atc gct gcc acc ccg gcg aca gct gga aac gga etc tat tcc att gac 211
Ile Ala Ala Thr Pro Ala Thr Ala Gly Asn Gly Leu Tyr Ser Ile Asp
25 30 35
atg ggc gac gag caa aag ctt acc tgc gtg ctt ttc gat gag ccc tcc 259
Met Gly Asp Glu Gln Lys Leu Thr Cys Val Leu Phe Asp Glu Pro Ser
40 45 50
acc gaa gcg cac gtc gtc gcc agc tgt gct gcg act ttc ccg gtg acc 307
Thr Glu Ala His Val Val Ala Ser Cys Ala Ala Thr Phe Pro Val Thr
55 60 65
tgg aag ctt ctc gac ggc gct cac gaa caa gcc gcg aaa ctt gaa atc 355
Trp Lys Leu Leu Asp Gly Ala His Glu Gln Ala Ala Lys Leu Glu Ile
70 75 80 85
acc cag gct caa gac ggt gaa ctc tcg gtg aca gcc agc aag cag ccg 403
Thr Gln Ala Gln Asp Gly Glu Leu Ser Val Thr Ala Ser Lys Gln Pro
90 95 100
ttg atc acc acg atg att gcg ccc acc agc atc act aag ccc atc act 451
Leu Ile Thr Thr Met Ile Ala Pro Thr Ser Ile Thr Lys Pro Ile Thr
105 1l0 115
gtc aat agg ctt gtg gtt gtt cct ggt gag aat gag gtt cgc ttt tat 499
Val Asn Arg Leu Val Val Val Pro Gly Glu Asn Glu Val Arg Phe Tyr
120 125 130
gct acc gat cct gat gtt tta cca gtg ctg atc acg cct gac tcc tat 547
Ala Thr Asp Pro Asp Val Leu Pro Val Leu Ile Thr Pro Asp Ser Tyr
135 140 145
gaa gtg ttg acc gat tcc gct gct aaa gtg aaa gcg aca tta 589
Glu Val Leu Thr Asp Ser Ala Ala Lys Val Lys Ala Thr Leu
150 155 160
tgaaataaac gtggatcaag gagtccggtg 619
<210>278
<211>163
<212>PRT
<213>谷氨酸棒状杆菌
<400>278
Met Arg Asn Gln Thr Ile Ala Ala Val Ala Ala Leu Val Leu Leu Thr
1 5 10 15
Ala Ala Thr Pro Ala Ile Ala Ala Thr Pro Ala Thr Ala Gly Asn Gly
20 25 30
Leu Tyr Ser Ile Asp Met Gly Asp Glu Gln Lys Leu Thr Cys Val Leu
35 40 45
Phe Asp Glu Pro Ser Thr Glu Ala His Val Val Ala Ser Cys Ala Ala
50 55 60
Thr Phe Pro Val Thr Trp Lys Leu Leu Asp Gly Ala His Glu Gln Ala
65 70 75 80
Ala Lys Leu Glu Ile Thr Gln Ala Gln Asp Gly Glu Leu Ser Val Thr
85 90 95
Ala Ser Lys Gln Pro Leu Ile Thr Thr Met Ile Ala Pro Thr Ser Ile
100 105 110
Thr Lys Pro Ile Thr Val Asn Arg Leu Val Val Val Pro Gly Glu Asn
115 120 125
Glu Val Arg Phe Tyr Ala Thr Asp Pro Asp Val Leu Pro Val Leu Ile
130 135 140
Thr Pro Asp Ser Tyr Glu Val Leu Thr Asp Ser Ala Ala Lys Val Lys
145 150 155 160
Ala Thr Leu
<210>279
<211>1789
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1759)
<223>RXA02298
<400>279
aaatcatagc gtgagatgac aaggaaagga atatttcgat ccggcaacaa cccccgcgtc 60
aggtgggatt atgccgaaaa aatcgctacg atagccactt atg act ctt cag agt 115
Met Thr Leu Gln Ser
1 5
tct gat aaa cgt tct cct tca ctg tta gac gcc tcc tcc gag gtc ttt 163
Ser Asp Lys Arg Ser Pro Ser Leu Leu Asp Ala Ser Ser Glu Val Phe
10 15 20
gtc cat gat ttg gca gct ttg tct ccc act gat gcc act gca tgg ggt 211
Val His Asp Leu Ala Ala Leu Ser Pro Thr Asp Ala Thr Ala Trp Gly
25 30 35
atc tca ggc ttc gaa ggt gat ctt cag gat ttc tct cct gac tat tgg 259
Ile Ser Gly Phe Glu Gly Asp Leu Gln Asp Phe Ser Pro Asp Tyr Trp
40 45 50
aat gcc atc gcc gaa cgc aac cgc gac atg gtt gcc gat gtt gat gct 307
Asn Ala Ile Ala Glu Arg Asn Arg Asp Met Val Ala Asp Val Asp Ala
55 60 65
ttt gat gac ggc act gat gac aac gat gac gaa gaa gat ttc gat gac 355
Phe Asp Asp Gly Thr Asp Asp Asn Asp Asp Glu Glu Asp Phe Asp Asp
70 75 80 85
gtt gac cgc gtc acc gct gat gtt ctc cgt gat cgc gta tgc ctg gat 403
Val Asp Arg Val Thr Ala Asp Val Leu Arg Asp Arg Val Cys Leu Asp
90 95 100
ctg gcc ctg cac cac cag ggc gaa acc tta gcg aat ctc aac aac att 451
Leu Ala Leu His His Gln Gly Glu Thr Leu Ala Asn Leu Asn Asn Ile
105 110 115
gat tcc cca gtc cag acc atc cgc gat acc ttc ctc atc atg ccc cgc 499
Asp Ser Pro Val Gln Thr Ile Arg Asp Thr Phe Leu Ile Met Pro Arg
120 125 130
gag acg gac gat gat gtg gaa aat ctc cgc gaa cgt ctc tcc cgc gtc 547
Glu Thr Asp Asp Asp Val Glu Asn Leu Arg Glu Arg Leu Ser Arg Val
135 140 145
ccc gac gct ctc cac gga tac tgt gaa tca ctc gct gag gct gcc agc 595
Pro Asp Ala Leu His Gly Tyr Cys Glu Ser Leu Ala Glu Ala Ala Ser
150 155 160 165
caa ggc cat gtt gca gcg gtc cgc cag gtg gaa gaa gtg gtg tcg caa 643
Gln Gly His Val Ala Ala Val Arg Gln Val Glu Glu Val Val Ser Gln
170 175 180
tgt gag gac ctc gct gat gag gac tcc gtt ttg cag cac ctt ggc ttg 691
Cys Glu Asp Leu Ala Asp Glu Asp Ser Val Leu Gln His Leu Gly Leu
185 190 195
gat gaa aat gat cca gta gtt gta gag gcc cag gaa gct ttt gca cga 739
Asp Glu Asn Asp Pro Val Val Val Glu Ala Gln Glu Ala Phe Ala Arg
200 205 210
gtt gcc ggt tgg tta gca gag caa ctg gct ccg cat gct cct cat gtg 787
Val Ala Gly Trp Leu Ala Glu Gln Leu Ala Pro His Ala Pro His Val
215 220 225
gat gcg gtt ggc cgg gat cgc tat gaa atg ttc tcc cac ctg cac gtc 835
Asp Ala Val Gly Arg Asp Arg Tyr Glu Met Phe Ser His Leu His Val
230 235 240 245
ggc gag ttt gta gat ctg gat gag gct tac caa tgg tcc ctg gaa cag 883
Gly Glu Phe Val Asp Leu Asp Glu Ala Tyr Gln Trp Ser Leu Glu Gln
250 255 260
ctg cgt gac att gat gct cag cag ctt cag gta gcc cag cag ctg tac 931
Leu Arg Asp Ile Asp Ala Gln Gln Leu Gln Val Ala Gln Gln Leu Tyr
265 270 275
ggc cct ggc acc acg atc cgt gag gcc atg aag aag ctc aat gct gat 979
Gly Pro Gly Thr Thr Ile Arg Glu Ala Met Lys Lys Leu Asn Ala Asp
280 285 290
gag cgc tac ctc atc cga ggc act gat gct ctg cag gag tgg atg caa 1027
Glu Arg Tyr Leu Ile Arg Gly Thr Asp Ala Leu Gln Glu Trp Met Gln
295 300 305
aag acc gct gat cag gca atc gct gat cta gat ggc gtt tcc ttc aac 1075
Lys Thr Ala Asp Gln Ala Ile Ala Asp Leu Asp Gly Val Ser Phe Asn
310 315 320 325
att cca gag cag gct cga cag gtt gag tgc ctc atc gat cct gct gga 1123
Ile Pro Glu Gln Ala Arg Gln Val Glu Cys Leu Ile Asp Pro Ala Gly
330 335 340
act ggc ggc att ttc tac acc cca cca agc gat gat ttc tcc agg cct 1171
Thr Gly Gly Ile Phe Tyr Thr Pro Pro Ser Asp Asp Phe Ser Arg Pro
345 350 355
ggc cgc atg tgg tgg tct gtg cct aaa acc cag gaa gtc ttc cac acc 1219
Gly Arg Met Trp Trp Ser Val Pro Lys Thr Gln Glu Val Phe His Thr
360 365 370
tgg caa gag ctc acc acc gtg ttc cac gag ggt gtt cct ggc cac cac 1267
Trp Gln Glu Leu Thr Thr Val Phe His Glu Gly Val Pro Gly His His
375 380 385
ctg cag atc tcc caa act ttg gtg gaa aag gat ctg aac ctg tgg cgt 1315
Leu Gln Ile Ser Gln Thr Leu Val Glu Lys Asp Leu Asn Leu Trp Arg
390 395 400 405
cga gtc gct tgc tgg aac tct ggc cac ggt gaa ggt tgg gcg ctg tac 1363
Arg Val Ala Cys Trp Asn Ser Gly His Gly Glu Gly Trp Ala Leu Tyr
410 415 420
gca gaa tct ctc atg aag gaa ttg gga tac cac gaa gat cca ggc aac 1411
Ala Glu Ser Leu Met Lys Glu Leu Gly Tyr His Glu Asp Pro Gly Asn
425 430 435
ctc atg ggc tac ctt gat gct cag cga ctc cgt gca gcc cgc gtg gcc 1459
Leu Met Gly Tyr Leu Asp Ala Gln Arg Leu Arg Ala Ala Arg Val Ala
440 445 450
att gac atc gga att cac ctg aac aag agg aac cca gag tgc acc ggc 1507
Ile Asp Ile Gly Ile His Leu Asn Lys Arg Asn Pro Glu Cys Thr Gly
455 460 465
ctg tgg gat gcg tct tat gcg cgc agc ttc ctg cgt gaa aat acg tcc 1555
Leu Trp Asp Ala Ser Tyr Ala Arg Ser Phe Leu Arg Glu Asn Thr Ser
470 475 480 485
atg aat gaa gat gcg ctg tac ttt gag ctc aac cgt tac ctt ggt tgg 1603
Met Asn Glu Asp Ala Leu Tyr Phe Glu Leu Asn Arg Tyr Leu Gly Trp
490 495 500
cca ggt cag gcc gca tcg tat gcc att ggt cag cga ctc tgg ttg aac 1651
Pro Gly Gln Ala Ala Ser Tyr Ala Ile Gly Gln Arg Leu Trp Leu Asn
505 510 515
ctg cgc gat gag gcc att agc cag ggg caa acc ttg gcg caa ttc cac 1699
Leu Arg Asp Glu Ala Ile Ser Gln Gly Gln Thr Leu Ala Gln Phe His
520 525 530
agc aag gca ttg tcc tat ggc agc atc ccg atg ggc att ttg cgc gat 1747
Ser Lys Ala Leu Ser Tyr Gly Ser Ile Pro Met Gly Ile Leu Arg Asp
535 540 545
cag gtg ctg aac tagaaggcct ttaacgatcc ctacgcaaaa 1789
Gln Val Leu Asn
550
<210>280
<211>553
<212>PRT
<213>谷氨酸棒状杆菌
<400>280
Met Thr Leu Gln Ser Ser Asp Lys Arg Ser Pro Ser Leu Leu Asp Ala
1 5 10 15
Ser Ser Glu Val Phe Val His Asp Leu Ala Ala Leu Ser Pro Thr Asp
20 25 30
Ala Thr Ala Trp Gly Ile Ser Gly Phe Glu Gly Asp Leu Gln Asp Phe
35 40 45
Ser Pro Asp Tyr Trp Asn Ala Ile Ala Glu Arg Asn Arg Asp Met Val
50 55 60
Ala Asp Val Asp Ala Phe Asp Asp Gly Thr Asp Asp Asn Asp Asp Glu
65 70 75 80
Glu Asp Phe Asp Asp Val Asp Arg Val Thr Ala Asp Val Leu Arg Asp
85 90 95
Arg Val Cys Leu Asp Leu Ala Leu His His Gln Gly Glu Thr Leu Ala
100 105 110
Asn Leu Asn Asn Ile Asp Ser Pro Val Gln Thr Ile Arg Asp Thr Phe
115 120 125
Leu Ile Met Pro Arg Glu Thr Asp Asp Asp Val Glu Asn Leu Arg Glu
130 135 140
Arg Leu Ser Arg Val Pro Asp Ala Leu His Gly Tyr Cys Glu Ser Leu
145 150 155 160
Ala Glu Ala Ala Ser Gln Gly His Val Ala Ala Val Arg Gln Val Glu
165 170 175
Glu Val Val Ser Gln Cys Glu Asp Leu Ala Asp Glu Asp Ser Val Leu
180 185 190
Gln His Leu Gly Leu Asp Glu Asn Asp Pro Val Val Val Glu Ala Gln
195 200 205
Glu Ala Phe Ala Arg Val Ala Gly Trp Leu Ala Glu Gln Leu Ala Pro
210 215 220
His Ala Pro His Val Asp Ala Val Gly Arg Asp Arg Tyr Glu Met Phe
225 230 235 240
Ser His Leu His Val Gly Glu Phe Val Asp Leu Asp Glu Ala Tyr Gln
245 250 255
Trp Ser Leu Glu Gln Leu Arg Asp Ile Asp Ala Gln Gln Leu Gln Val
260 265 270
Ala Gln Gln Leu Tyr Gly Pro Gly Thr Thr Ile Arg Glu Ala Met Lys
275 280 285
Lys Leu Asn Ala Asp Glu Arg Tyr Leu Ile Arg Gly Thr Asp Ala Leu
290 295 300
Gln Glu Trp Met Gln Lys Thr Ala Asp Gln Ala Ile Ala Asp Leu Asp
305 310 315 320
Gly Val Ser Phe Asn Ile Pro Glu Gln Ala Arg Gln Val Glu Cys Leu
325 330 335
Ile Asp Pro Ala Gly Thr Gly Gly Ile Phe Tyr Thr Pro Pro Ser Asp
340 345 350
Asp Phe Ser Arg Pro Gly Arg Met Trp Trp Ser Val Pro Lys Thr Gln
355 360 365
Glu Val Phe His Thr Trp Gln Glu Leu Thr Thr Val Phe His Glu Gly
370 375 380
Val Pro Gly His His Leu Gln Ile Ser Gln Thr Leu Val Glu Lys Asp
385 390 395 400
Leu Asn Leu Trp Arg Arg Val Ala Cys Trp Asn Ser Gly His Gly Glu
405 410 415
Gly Trp Ala Leu Tyr Ala Glu Ser Leu Met Lys Glu Leu Gly Tyr His
420 425 430
Glu Asp Pro Gly Asn Leu Met Gly Tyr Leu Asp Ala Gln Arg Leu Arg
435 440 445
Ala Ala Arg Val Ala Ile Asp Ile Gly Ile His Leu Asn Lys Arg Asn
450 455 460
Pro Glu Cys Thr Gly Leu Trp Asp Ala Ser Tyr Ala Arg Ser Phe Leu
465 470 475 480
Arg Glu Asn Thr Ser Met Asn Glu Asp Ala Leu Tyr Phe Glu Leu Asn
485 490 495
Arg Tyr Leu Gly Trp Pro Gly Gln Ala Ala Ser Tyr Ala Ile Gly Gln
500 505 510
Arg Leu Trp Leu Asn Leu Arg Asp Glu Ala Ile Ser Gln Gly Gln Thr
515 520 525
Leu Ala Gln Phe His Ser Lys Ala Leu Ser Tyr Gly Ser Ile Pro Met
530 535 540
Gly Ile Leu Arg Asp Gln Val Leu Asn
545 550
<210>281
<211>1009
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(979)
<223>RXA02302
<400>281
ggccaggttc ggttagtgcg cgatctccct gatctctgac tcatacatcc aataagatcc 60
agacatatgc gaagctaatg aaggaaacga ggagctgata gtg gca aca aat cgt 115
Val Ala Thr Asn Arg
1 5
aca tct tcc gct ggt gtc atc acg tct gtt ctc gca tcc gct ttg ttc 163
Thr Ser Ser Ala Gly Val Ile Thr Ser Val Leu Ala Ser Ala Leu Phe
10 15 20
gga gcg atc ttt ttc atc tct ggg gcg att gag gca aag gcg gaa aca 211
Gly Ala Ile Phe Phe Ile Ser Gly Ala Ile Glu Ala Lys Ala Glu Thr
25 30 35
ctt gtt gct tgg cgt gtc ctt cta aca gcg gca tgc tat ttg ctt gcc 259
Leu Val Ala Trp Arg Val Leu Leu Thr Ala Ala Cys Tyr Leu Leu Ala
40 45 50
ctt ctg cac cct gct ggt cgg aag gtg ttt aag gaa ttt tgg gac aca 307
Leu Leu His Pro Ala Gly Arg Lys Val Phe Lys Glu Phe Trp Asp Thr
55 60 65
cta agg tcc caa cct cgt caa att ctg tac ttt atc ttc ctc gtt gta 355
Leu Arg Ser Gln Pro Arg Gln Ile Leu Tyr Phe Ile Phe Leu Val Val
70 75 80 85
ctc atc acg ctt cag ctg tgg ctg ttt tct tgg tcg cct aag agt cat 403
Leu Ile Thr Leu Gln Leu Trp Leu Phe Ser Trp Ser Pro Lys Ser His
90 95 100
gct tta gat gcc tca ctt ggt tac ctg tta ctg ccc att ttc cta gta 451
Ala Leu Asp Ala Ser Leu Gly Tyr Leu Leu Leu Pro Ile Phe Leu Val
105 110 115
att gtg ggg cgg ttt ttc ttc gct gat tac atc acc agg ctg cag tgg 499
Ile Val Gly Arg Phe Phe Phe Ala Asp Tyr Ile Thr Arg Leu Gln Trp
120 125 130
att gcg gtg gga atc gcg ctg atc gca gta acc ttg aaa ttt gtc att 547
Ile Ala Val Gly Ile Ala Leu Ile Ala Val Thr Leu Lys Phe Val Ile
135 140 145
agt gcg caa tta tcg tgg gta act ttt gcc atc gct gcg ggt tat gca 595
Ser Ala Gln Leu Ser Trp Val Thr Phe Ala Ile Ala Ala Gly Tyr Ala
150 155 160 165
ctg tat ttt gcg ctt cgt aaa tac tcg ggg ctg aac aac gct ttt gct 643
Leu Tyr Phe Ala Leu Arg Lys Tyr Ser Gly Leu Asn Asn Ala Phe Ala
170 175 180
tac ggc gca gaa gtt tta gcg tta agt ccc ctg gca ttt ttc atg ctc 691
Tyr Gly Ala Glu Val Leu Ala Leu Ser Pro Leu Ala Phe Phe Met Leu
185 190 195
gcc act gtc gag gat ccg ctg tct aac gcc atg ttg tca atg gtt att 739
Ala Thr Val Glu Asp Pro Leu Ser Asn Ala Met Leu Ser Met Val Ile
200 205 210
ctg gcg ggc ctc gct ggc gca tta gct atg gcg ttg tat cta gcc gcc 787
Leu Ala Gly Leu Ala Gly Ala Leu Ala Met Ala Leu Tyr Leu Ala Ala
215 220 225
tca act ctg ctt agt atg ccg atg ttt ggc ctg ctc agt tat ggt gaa 835
Ser Thr Leu Leu Ser Met Pro Met Phe Gly Leu Leu Ser Tyr Gly Glu
230 235 240 245
cca atc ttg ctg ttt gtt gca gca cta tta ctt ggt gaa acg ttg aat 883
Pro Ile Leu Leu Phe Val Ala Ala Leu Leu Leu Gly Glu Thr Leu Asn
250 255 260
ctc agc gac gcc att gtc tac tca cta ctg gcg tgc gcc ctg gcg tta 931
Leu Ser Asp Ala Ile Val Tyr Ser Leu Leu Ala Cys Ala Leu Ala Leu
265 270 275
ctg gga ttt gat ggg ata cga agg tct cga aaa aac ctc gag acc gat 979
Leu Gly Phe Asp Gly Ile Arg Arg Ser Arg Lys Asn Leu Glu Thr Asp
280 285 290
taacttctgc tcaacttccc accaattaag 1009
<210>282
<211>293
<212>PRT
<213>谷氨酸棒状杆菌
<400>282
Val Ala Thr Asn Arg Thr Ser Ser Ala Gly Val Ile Thr Ser Val Leu
1 5 10 15
Ala Ser Ala Leu Phe Gly Ala Ile Phe Phe Ile Ser Gly Ala Ile Glu
20 25 30
Ala Lys Ala Glu Thr Leu Val Ala Trp Arg Val Leu Leu Thr Ala Ala
35 40 45
Cys Tyr Leu Leu Ala Leu Leu His Pro Ala Gly Arg Lys Val Phe Lys
50 55 60
Glu Phe Trp Asp Thr Leu Arg Ser Gln Pro Arg Gln Ile Leu Tyr Phe
65 70 75 80
Ile Phe Leu Val Val Leu Ile Thr Leu Gln Leu Trp Leu Phe Ser Trp
85 90 95
Ser Pro Lys Ser His Ala Leu Asp Ala Ser Leu Gly Tyr Leu Leu Leu
100 105 110
Pro Ile Phe Leu Val Ile Val Gly Arg Phe Phe Phe Ala Asp Tyr Ile
115 120 125
Thr Arg Leu Gln Trp Ile Ala Val Gly Ile Ala Leu Ile Ala Val Thr
130 135 140
Leu Lys Phe Val Ile Ser Ala Gln Leu Ser Trp Val Thr Phe Ala Ile
145 150 155 160
Ala Ala Gly Tyr Ala Leu Tyr Phe Ala Leu Arg Lys Tyr Ser Gly Leu
165 170 175
Asn Asn Ala Phe Ala Tyr Gly Ala Glu Val Leu Ala Leu Ser Pro Leu
180 185 190
Ala Phe Phe Met Leu Ala Thr Val Glu Asp Pro Leu Ser Asn Ala Met
195 200 205
Leu Ser Met Val Ile Leu Ala Gly Leu Ala Gly Ala Leu Ala Met Ala
210 215 220
Leu Tyr Leu Ala Ala Ser Thr Leu Leu Ser Met Pro Met Phe Gly Leu
225 230 235 240
Leu Ser Tyr Gly Glu Pro Ile Leu Leu Phe Val Ala Ala Leu Leu Leu
245 250 255
Gly Glu Thr Leu Asn Leu Ser Asp Ala Ile Val Tyr Ser Leu Leu Ala
260 265 270
Cys Ala Leu Ala Leu Leu Gly Phe Asp Gly Ile Arg Arg Ser Arg Lys
275 280 285
Asn Leu Glu Thr Asp
290
<210>283
<211>1036
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1006)
<223>RXA02303
<400>283
gcccgggccg aaatcattcc aatctcaccc ccacccaaaa cactgttgat attttcactt 60
tccaaccagc gacgtgagcg cacgcactaa ggttgtgccc atg gct gaa aac aac 115
Met Ala Glu Asn Asn
1 5
acc cca caa gaa act gaa caa gat cag gaa gca cgc ttc cgt gag gag 163
Thr Pro Gln Glu Thr Glu Gln Asp Gln Glu Ala Arg Phe Arg Glu Glu
10 15 20
ttc gag gtt ggc ggt aag gat cgt cag ctt tct gaa gaa gaa cag ctg 211
Phe Glu Val Gly Gly Lys Asp Arg Gln Leu Ser Glu Glu Glu Gln Leu
25 30 35
gag cag ctc gga tcg tat att gat gcg cac tac cct gtt cca gat ttc 259
Glu Gln Leu Gly Ser Tyr Ile Asp Ala His Tyr Pro Val Pro Asp Phe
40 45 50
acg ccc cct tgg gca ggt ggc gcc ggc gat cct gat cca gca gac cgc 307
Thr Pro Pro Trp Ala Gly Gly Ala Gly Asp Pro Asp Pro Ala Asp Arg
55 60 65
tat att gca cat ctt ccc gat cgc acc acg cat act gcg atg atc atg 355
Tyr Ile Ala His Leu Pro Asp Arg Thr Thr His Thr Ala Met Ile Met
70 75 80 85
ttg ggt tct ggc ctt gat cat tcc atg ccg ggt gtc gca ttt atc ggt 403
Leu Gly Ser Gly Leu Asp His Ser Met Pro Gly Val Ala Phe Ile Gly
90 95 100
ggt gtg agc gtc gat gac gtc ccg gag gtc gga ggc gcg att ttc cac 451
Gly Val Ser Val Asp Asp Val Pro Glu Val Gly Gly Ala Ile Phe His
105 110 115
ccg tcg aat ccg acg ggc cgc tgg gct gta tcg ttc cac agc ggt ggt 499
Pro Ser Asn Pro Thr Gly Arg Trp Ala Val Ser Phe His Ser Gly Gly
120 125 130
tgg tgg cgc ggt tcc ggt gat gcg ttg gag ttc cag tgg cgt cca gag 547
Trp Trp Arg Gly Ser Gly Asp Ala Leu Glu Phe Gln Trp Arg Pro Glu
135 140 145
gtc gcg gca gct gcg gaa ttg tcc ggc aca acc atc ctt gac ctg gac 595
Val Ala Ala Ala Ala Glu Leu Ser Gly Thr Thr Ile Leu Asp Leu Asp
150 155 160 165
tat ccg ctg gcc cca ggc cat aac ctc cac gat atg aat gag gtt gtg 643
Tyr Pro Leu Ala Pro Gly His Asn Leu His Asp Met Asn Glu Val Val
170 175 180
ggc aag gcg gtc ggt tac gcc cgc cac cat aat ccg gtg tcg atc acg 691
Gly Lys Ala Val Gly Tyr Ala Arg His His Asn Pro Val Ser Ile Thr
185 190 195
ggt tgg ggt tat tcc tcg ggc gcg gcg tta gcc gcg att aat gca tcg 739
Gly Trp Gly Tyr Ser Ser Gly Ala Ala Leu Ala Ala Ile Asn Ala Ser
200 205 210
ctt ttc gac gcc ctg gtc ctc acc ttt ccg gac ctg ggt agc gtc gag 787
Leu Phe Asp Ala Leu Val Leu Thr Phe Pro Asp Leu Gly Ser Val Glu
215 220 225
aag ctc cct gcc gaa att cgt gga gac gct gta gtg ccc gcg gcg gct 835
Lys Leu Pro Ala Glu Ile Arg Gly Asp Ala Val Val Pro Ala Ala Ala
230 235 240 245
gcg tgg ccg acg acc ttt gtg cag atc gcg gcg cag gat gag atc gcg 883
Ala Trp Pro Thr Thr Phe Val Gln Ile Ala Ala Gln Asp Glu Ile Ala
250 255 260
gag cgg cca gga gag ctt ggc gac gcg acc gtg aag gaa tac gtt tcc 931
Glu Arg Pro Gly Glu Leu Gly Asp Ala Thr Val Lys Glu Tyr Val Ser
265 270 275
cgc cac cgt att tcc aca ccg aag gtc gcg cgc gaa aaa atc acc gac 979
Arg His Arg Ile Ser Thr Pro Lys Val Ala Arg Glu Lys Ile Thr Asp
280 285 290
gtg gcg gag ttc ctt aaa act gtt tgc taatgtgcca gcaggttgta 1026
Val Ala Glu Phe Leu Lys Thr Val Cys
295 300
aataaacgct 1036
<210>284
<211>302
<212>PRT
<213>谷氨酸棒状杆菌
<400>284
Met Ala Glu Asn Asn Thr Pro Gln Glu Thr Glu Gln Asp Gln Glu Ala
1 5 10 15
Arg Phe Arg Glu Glu Phe Glu Val Gly Gly Lys Asp Arg Gln Leu Ser
20 25 30
Glu Glu Glu Gln Leu Glu Gln Leu Gly Ser Tyr Ile Asp Ala His Tyr
35 40 45
Pro Val Pro Asp Phe Thr Pro Pro Trp Ala Gly Gly Ala Gly Asp Pro
50 55 60
Asp Pro Ala Asp Arg Tyr Ile Ala His Leu Pro Asp Arg Thr Thr His
65 70 75 80
Thr Ala Met Ile Met Leu Gly Ser Gly Leu Asp His Ser Met Pro Gly
85 90 95
Val Ala Phe Ile Gly Gly Val Ser Val Asp Asp Val Pro Glu Val Gly
100 105 110
Gly Ala Ile Phe His Pro Ser Asn Pro Thr Gly Arg Trp Ala Val Ser
115 120 125
Phe His Ser Gly Gly Trp Trp Arg Gly Ser Gly Asp Ala Leu Glu Phe
130 135 140
Gln Trp Arg Pro Glu Val Ala Ala Ala Ala Glu Leu Ser Gly Thr Thr
145 150 155 160
Ile Leu Asp Leu Asp Tyr Pro Leu Ala Pro Gly His Asn Leu His Asp
165 170 175
Met Asn Glu Val Val Gly Lys Ala Val Gly Tyr Ala Arg His His Asn
180 185 190
Pro Val Ser Ile Thr Gly Trp Gly Tyr Ser Ser Gly Ala Ala Leu Ala
195 200 205
Ala Ile Asn Ala Ser Leu Phe Asp Ala Leu Val Leu Thr Phe Pro Asp
210 215 220
Leu Gly Ser Val Glu Lys Leu Pro Ala Glu Ile Arg Gly Asp Ala Val
225 230 235 240
Val Pro Ala Ala Ala Ala Trp Pro Thr Thr Phe Val Gln Ile Ala Ala
245 250 255
Gln Asp Glu Ile Ala Glu Arg Pro Gly Glu Leu Gly Asp Ala Thr Val
260 265 270
Lys Glu Tyr Val Ser Arg His Arg Ile Ser Thr Pro Lys Val Ala Arg
275 280 285
Glu Lys Ile Thr Asp Val Ala Glu Phe Leu Lys Thr Val Cys
290 295 300
<210>285
<211>544
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(514)
<223>RXA02339
<400>285
ttgagaaagc cctctcgccg ttcccaccga caacctcttt tcatatcctg cacaacgcta 60
tgatcttctc acacttgcct ttgaagttag gattggggac atg gtt caa att aat 115
Met Val Gln Ile Asn
1 5
gac atg ctt gcc cct cct cca gta aaa ctt ccg gaa gat cct gcc ctc 163
Asp Met Leu Ala Pro Pro Pro Val Lys Leu Pro Glu Asp Pro Ala Leu
10 15 20
ggc gcc gat cca act ttg acc tcg aca gcg att gcg cat cct gac agc 211
Gly Ala Asp Pro Thr Leu Thr Ser Thr Ala Ile Ala His Pro Asp Ser
25 30 35
cca ttg gtg tgg gcg tac cga gct gaa aat ctt atc aaa tct gca tca 259
Pro Leu Val Trp Ala Tyr Arg Ala Glu Asn Leu Ile Lys Ser Ala Ser
40 45 50
aat gat gaa gag aag att cag gcc tac gct ttt gcg cgc acg ggt tac 307
Asn Asp Glu Glu Lys Ile Gln Ala Tyr Ala Phe Ala Arg Thr Gly Tyr
55 60 65
cac cgc agt ctc gat cgt ctg cgt gcc aat ggt tgg aag ggt tgg ggt 355
His Arg Ser Leu Asp Arg Leu Arg Ala Asn Gly Trp Lys Gly Trp Gly
70 75 80 85
cct gtc ccc ttc tct cat gag cca aac cag gga gtg ttg cgg gct atc 403
Pro Val Pro Phe Ser His Glu Pro Asn Gln Gly Val Leu Arg Ala Ile
90 95 100
gct tcc cta gct ctt gct gcg aag ctg att ggt gag gac aac gaa tac 451
Ala Ser Leu Ala Leu Ala Ala Lys Leu Ile Gly Glu Asp Asn Glu Tyr
105 110 115
gat cgt tgc cgc cag atg ctc tct gat gcg gat cca gag tcc gtc gca 499
Asp Arg Cys Arg Gln Met Leu Ser Asp Ala Asp Pro Glu Ser Val Ala
120 125 130
gtc cta ctc gac aaa taaaccacca aaaataaaac aatctccctc 544
Val Leu Leu Asp Lys
135
<210>286
<211>138
<212>PRT
<213>谷氨酸棒状杆菌
<400>286
Met Val Gln Ile Asn Asp Met Leu Ala Pro Pro Pro Val Lys Leu Pro
1 5 10 15
Glu Asp Pro Ala Leu Gly Ala Asp Pro Thr Leu Thr Ser Thr Ala Ile
20 25 30
Ala His Pro Asp Ser Pro Leu Val Trp Ala Tyr Arg Ala Glu Asn Leu
35 40 45
Ile Lys Ser Ala Ser Asn Asp Glu Glu Lys Ile Gln Ala Tyr Ala Phe
50 55 60
Ala Arg Thr Gly Tyr His Arg Ser Leu Asp Arg Leu Arg Ala Asn Gly
65 70 75 80
Trp Lys Gly Trp Gly Pro Val Pro Phe Ser His Glu Pro Asn Gln Gly
85 90 95
Val Leu Arg Ala Ile Ala Ser Leu Ala Leu Ala Ala Lys Leu Ile Gly
100 105 110
Glu Asp Asu Glu Tyr Asp Arg Cys Arg Gln Met Leu Ser Asp Ala Asp
115 120 125
Pro Glu Ser Val Ala Val Leu Leu Asp Lys
130 135
<210>287
<211>2563
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2533)
<223>RXA02360
<400>287
aagtaactaa agttcgaaag tatttccgaa cggtgtcggc ctctgcgcat acactgtatt 60
tttaaagaaa attcttctca attctaaggg tgaatatcca atg cgt ggt gac gtt 1l5
Met Arg Gly Asp Val
1 5
caa aaa ata acc aag gtt tat gac ggc aag cac cga acc ttg gaa atc 163
Gln Lys Ile Thr Lys Val Tyr Asp Gly Lys His Arg Thr Leu Glu Ile
10 15 20
ccg gtt tat caa cgc aat tac gac tgg act gaa aag caa tgt tca cgc 211
Pro Val Tyr Gln Arg Asn Tyr Asp Trp Thr Glu Lys Gln Cys Ser Arg
25 30 35
ctc ttt gat gac ctc agc gaa gtg gtc aaa gaa aac cac cgt caa cat 259
Leu Phe Asp Asp Leu Ser Glu Val Val Lys Glu Asn His Arg Gln His
40 45 50
ttt ttt gga gct gtg gtt ggt aag cct caa ggc agc tgg acc tgg gtt 307
Phe Phe Gly Ala Val Val Gly Lys Pro Gln Gly Ser Trp Thr Trp Val
55 60 65
gtc atc gat gga cag cag cgt ctt aca aca atc agc ttg ttc atg ctt 355
Val Ile Asp Gly Gln Gln Arg Leu Thr Thr Ile Ser Leu Phe Met Leu
70 75 80 85
gcg ctg gta cat tcc ttg cgc gca tct gaa gtt gaa gaa ggt gag ttt 403
Ala Leu Val His Ser Leu Arg Ala Ser Glu Val Glu Glu Gly Glu Phe
90 95 100
aat gct ggc tac aac att gat cta gcc aca ttg att gag gat gac tac 451
Asn Ala Gly Tyr Asn Ile Asp Leu Ala Thr Leu Ile Glu Asp Asp Tyr
105 110 115
ctc cga tcc ggc aat gag gga aac ctc aag ttc aag ctc aag cca gtt 499
Leu Arg Ser Gly Asn Glu Gly Asn Leu Lys Phe Lys Leu Lys Pro Val
120 125 130
aaa aac gat aat gag gct tat caa aag ctc ttc ggc cca gaa tct gaa 547
Lys Asn Asp Asn Glu Ala Tyr Gln Lys Leu Phe Gly Pro Glu Ser Glu
135 140 145
ttc ata gag tcc tca aac ctc act gct aat tat cgc tac ttc cgc aat 595
Phe Ile Glu Ser Ser Asn Leu Thr Ala Asn Tyr Arg Tyr Phe Arg Asn
150 155 160 165
gtc ctt aag gca acc gat ctc acc gca gct caa cta tgg gaa gct atc 643
Val Leu Lys Ala Thr Asp Leu Thr Ala Ala Gln Leu Trp Glu Ala Ile
170 175 180
gag aaa ctc agg gtc atg tat ctt gac ctt gag gaa ttt gat gat ccg 691
Glu Lys Leu Arg Val Met Tyr Leu Asp Leu Glu Glu Phe Asp Asp Pro
185 190 195
cag cga atc ttt gaa agt ctc aac tcc acg gga tta gag ctc agt gaa 739
Gln Arg Ile Phe Glu Ser Leu Asn Ser Thr Gly Leu Glu Leu Ser Glu
200 205 210
gct gat aag gta cgc aac ttg gtt ctt atg gat cag gag ctc aaa acc 787
Ala Asp Lys Val Arg Asn Leu Val Leu Met Asp Gln Glu Leu Lys Thr
215 220 225
caa gaa aag ctc tat gag cag cgt tgg aat ccc att gaa gtc tgc gtg 835
Gln Glu Lys Leu Tyr Glu Gln Arg Trp Asn Pro Ile Glu Val Cys Val
230 235 240 245
aaa ttt gat acc gac aat ttc att cgc tgg tac tta act ctc aaa acc 883
Lys Phe Asp Thr Asp Asn Phe Ile Arg Trp Tyr Leu Thr Leu Lys Thr
250 255 260
gcg cgc acc cca aga aag caa gat gtt tat gag gaa ttc aag aaa ttc 931
Ala Arg Thr Pro Arg Lys Gln Asp Val Tyr Glu Glu Phe Lys Lys Phe
265 270 275
atc cgt aac tcc aag ctc ccg gtc gaa ttc atc ctc gac gat atg tac 979
Ile Arg Asn Ser Lys Leu Pro Val Glu Phe Ile Leu Asp Asp Met Tyr
280 285 290
gag tac gcg aag ctg tac cga gat ctc tta ggt gcc act act ggg ttt 1027
Glu Tyr Ala Lys Leu Tyr Arg Asp Leu Leu Gly Ala Thr Thr Gly Phe
295 300 305
ata gct gcc gat agg tgt ctt aag cgt ttt gtc cct gtc atg ggc gat 1075
Ile Ala Ala Asp Arg Cys Leu Lys Arg Phe Val Pro Val Met Gly Asp
310 315 320 325
gtg gtt ttg cct ttt ctg ctg cct gtt ctc aaa gat gct aaa gac ggc 1123
Val Val Leu Pro Phe Leu Leu Pro Val Leu Lys Asp Ala Lys Asp Gly
330 335 340
atc atc acc gaa tca gac ttc ctc gga gtt cta aaa gtt ctg gaa tcc 1171
Ile Ile Thr Glu Ser Asp Phe Leu Gly Val Leu Lys Val Leu Glu Ser
345 350 355
tat tta ttc cgc cgt ttt gca gtc ggg gtt gct agt aac gcc tta agc 1219
Tyr Leu Phe Arg Arg Phe Ala Val Gly Val Ala Ser Asn Ala Leu Ser
360 365 370
aag atc ttc tcc act gct tat agc gac atc aag aaa ttc tgg act cca 1267
Lys Ile Phe Ser Thr Ala Tyr Ser Asp Ile Lys Lys Phe Trp Thr Pro
375 380 385
ggg caa agt tat agc tca ctg ctg gcc tac att ctg aaa cgt cgc gat 1315
Gly Gln Ser Tyr Ser Ser Leu Leu Ala Tyr Ile Leu Lys Arg Arg Asp
390 395 400 405
ggt tcc ggg cgt ttt ccc tcc gac agt gaa ttc cgt gag aac ttc gca 1363
Gly Ser Gly Arg Phe Pro Ser Asp Ser Glu Phe Arg Glu Asn Phe Ala
410 415 420
acc aaa aat ttc tgg aat att cat aac gaa aac agg cgt tac tta ttt 1411
Thr Lys Asn Phe Trp Asn Ile His Asn Glu Asn Arg Arg Tyr Leu Phe
425 430 435
gat tgt cta gaa aat gcc gat tcc aat gat gtt cgt gac atc caa aca 1459
Asp Cys Leu Glu Asn Ala Asp Ser Asn Asp Val Arg Asp Ile Gln Thr
440 445 450
agt ctg gat gaa gga tct ctc tcc att gaa cac atc atg cca cgt agc 1507
Ser Leu Asp Glu Gly Ser Leu Ser Ile Glu His Ile Met Pro Arg Ser
455 460 465
cta aac gat cag tgg aga gct gaa ctc ggc cct gaa tat gct cga att 1555
Leu Asn Asp Gln Trp Arg Ala Glu Leu Gly Pro Glu Tyr Ala Arg Ile
470 475 480 485
cat gag act tgg att aat aga att ggt aac ctc acc atc acc ggc tac 1603
His Glu Thr Trp Ile Asn Arg Ile Gly Asn Leu Thr Ile Thr Gly Tyr
490 495 500
aac tcc gcc tat tca aat tct tcc tat gag cgc aag cgg acg atg gaa 1651
Asn Ser Ala Tyr Ser Asn Ser Ser Tyr Glu Arg Lys Arg Thr Met Glu
505 510 515
aat gga ttt ttg gta tcc ccc tat cgc atc aat aat ttc atc aaa aag 1699
Asn Gly Phe Leu Val Ser Pro Tyr Arg Ile Asn Asn Phe Ile Lys Lys
520 525 530
cag aag cat tgg tct gaa gaa cag cta att gag cga acc gag ctg ctc 1747
Gln Lys His Trp Ser Glu Glu Gln Leu Ile Glu Arg Thr Glu Leu Leu
535 540 545
act caa gca gcg ctg gac tac tgg ccc ctt cca aaa gaa acc ttc cag 1795
Thr Gln Ala Ala Leu Asp Tyr Trp Pro Leu Pro Lys Glu Thr Phe Gln
550 555 560 565
cca cca caa gct gtg ctt cct aca gaa tcc tta gat agt gat ctt tct 1843
Pro Pro Gln Ala Val Leu Pro Thr Glu Ser Leu Asp Ser Asp Leu Ser
570 575 580
ttc cgc gga cgc gaa att gtc gcc ttt gaa tat gag gac tac aag gaa 1891
Phe Arg Gly Arg Glu Ile Val Ala Phe Glu Tyr Glu Asp Tyr Lys Glu
585 590 595
acg gta acg tcc tgg gca gac atg ctc cag agc gtt cta aaa gtc ttg 1939
Thr Val Thr Ser Trp Ala Asp Met Leu Gln Ser Val Leu Lys Val Leu
600 605 610
aac caa tcc ttc cgc cag gaa ctc ata gct ttg acc aat gaa gaa atc 1987
Asn Gln Ser Phe Arg Gln Glu Leu Ile Ala Leu Thr Asn Glu Glu Ile
615 620 625
tgc ctg gca act tca aac aac agt aac agc agt ctc cgt gaa att gac 2035
Cys Leu Ala Thr Ser Asn Asn Ser Asn Ser Ser Leu Arg Glu Ile Asp
630 635 640 645
cac ggg ctt ttt gta gac act ggc tct agt acg agt gta aag att ggc 2083
His Gly Leu Phe Val Asp Thr Gly Ser Ser Thr Ser Val Lys Ile Gly
650 655 660
ttc ctc cgc aga gtt ttt act caa ctg ggg cta gag cag gaa gca ttg 2131
Phe Leu Arg Arg Val Phe Thr Gln Leu Gly Leu Glu Gln Glu Ala Leu
665 670 675
gtt ttc acc ctt cga cca ctg gca aat gat gta gaa ccc cga gat gat 2179
Val Phe Thr Leu Arg Pro Leu Ala Asn Asp Val Glu Pro Arg Asp Asp
680 685 690
gaa ctc gag gta gag gta gaa aag aaa tat tcc gat ttg act aaa ttc 2227
Glu Leu Glu Val Glu Val Glu Lys Lys Tyr Ser Asp Leu Thr Lys Phe
695 700 705
atc cca cag tta gaa gaa gct gag aat ctc gag ggg gcg gat aca gag 2275
Ile Pro Gln Leu Glu Glu Ala Glu Asn Leu Glu Gly Ala Asp Thr Glu
710 715 720 725
gtt att cct ctc ctc tca aag ctc aag gaa caa ctg aca gcc ttt agc 2323
Val Ile Pro Leu Leu Ser Lys Leu Lys Glu Gln Leu Thr Ala Phe Ser
730 735 740
ccg gag aat cct caa gca gcc cta ggg ggc cta cct gtt cca gaa ttc 2371
Pro Glu Asn Pro Gln Ala Ala Leu Gly Gly Leu Pro Val Pro Glu Phe
745 750 755
ctc aaa caa aat gtg att gaa caa cta agc gca gag cac atc ctt gct 2419
Leu Lys Gln Asn Val Ile Glu Gln Leu Ser Ala Glu His Ile Leu Ala
760 765 770
gta ctc act caa cac ttt aat atc gca tcc atg atg ggt gat gac tat 2467
Val Leu Thr Gln His Phe Asn Ile Ala Ser Met Met Gly Asp Asp Tyr
775 780 785
ttg ctc gaa gag ctc agg tca ggc cgc tta agg gaa tta ctg cag cgt 2515
Leu Leu Glu Glu Leu Arg Ser Gly Arg Leu Arg Glu Leu Leu Gln Arg
790 795 800 805
tta gag gaa ttg gat agc taatagccgt agtcggtggt caaactttga 2563
Leu Glu Glu Leu Asp Ser
810
<210>288
<211>811
<212>PRT
<213>谷氨酸棒状杆菌
<400>288
Met Arg Gly Asp Val Gln Lys Ile Thr Lys Val Tyr Asp Gly Lys His
1 5 10 15
Arg Thr Leu Glu Ile Pro Val Tyr Gln Arg Asn Tyr Asp Trp Thr Glu
20 25 30
Lys Gln Cys Ser Arg Leu Phe Asp Asp Leu Ser Glu Val Val Lys Glu
35 40 45
Asn His Arg Gln His Phe Phe Gly Ala Val Val Gly Lys Pro Gln Gly
50 55 60
Ser Trp Thr Trp Val Val Ile Asp Gly Gln Gln Arg Leu Thr Thr Ile
65 70 75 80
Ser Leu Phe Met Leu Ala Leu Val His Ser Leu Arg Ala Ser Glu Val
85 90 95
Glu Glu Gly Glu Phe Asn Ala Gly Tyr Asn Ile Asp Leu Ala Thr Leu
100 105 110
Ile Glu Asp Asp Tyr Leu Arg Ser Gly Asn Glu Gly Asn Leu Lys Phe
115 120 125
Lys Leu Lys Pro Val Lys Asn Asp Asn Glu Ala Tyr Gln Lys Leu Phe
130 135 140
Gly Pro Glu Ser Glu Phe Ile Glu Ser Ser Asn Leu Thr Ala Asn Tyr
145 150 155 160
Arg Tyr Phe Arg Asn Val Leu Lys Ala Thr Asp Leu Thr Ala Ala Gln
165 170 175
Leu Trp Glu Ala Ile Glu Lys Leu Arg Val Met Tyr Leu Asp Leu Glu
180 185 190
Glu Phe Asp Asp Pro Gln Arg Ile Phe Glu Ser Leu Asn Ser Thr Gly
195 200 205
Leu Glu Leu Ser Glu Ala Asp Lys Val Arg Asn Leu Val Leu Met Asp
210 215 220
Gln Glu Leu Lys Thr Gln Glu Lys Leu Tyr Glu Gln Arg Trp Asn Pro
225 230 235 240
Ile Glu Val Cys Val Lys Phe Asp Thr Asp Asn Phe Ile Arg Trp Tyr
245 250 255
Leu Thr Leu Lys Thr Ala Arg Thr Pro Arg Lys Gln Asp Val Tyr Glu
260 265 270
Glu Phe Lys Lys Phe Ile Arg Asn Ser Lys Leu Pro Val Glu Phe Ile
275 280 285
Leu Asp Asp Met Tyr Glu Tyr Ala Lys Leu Tyr Arg Asp Leu Leu Gly
290 295 300
Ala Thr Thr Gly Phe Ile Ala Ala Asp Arg Cys Leu Lys Arg Phe Val
305 310 315 320
Pro Val Met Gly Asp Val Val Leu Pro Phe Leu Leu Pro Val Leu Lys
325 330 335
Asp Ala Lys Asp Gly Ile Ile Thr Glu Ser Asp Phe Leu Gly Val Leu
340 345 350
Lys Val Leu Glu Ser Tyr Leu Phe Arg Arg Phe Ala Val Gly Val Ala
355 360 365
Ser Asn Ala Leu Ser Lys Ile Phe Ser Thr Ala Tyr Ser Asp Ile Lys
370 375 380
Lys Phe Trp Thr Pro Gly Gln Ser Tyr Ser Ser Leu Leu Ala Tyr Ile
385 390 395 400
Leu Lys Arg Arg Asp Gly Ser Gly Arg Phe Pro Ser Asp Ser Glu Phe
405 410 415
Arg Glu Asn Phe Ala Thr Lys Asn Phe Trp Asn Ile His Asn Glu Asn
420 425 430
Arg Arg Tyr Leu Phe Asp Cys Leu Glu Asn Ala Asp Ser Asn Asp Val
435 440 445
Arg Asp Ile Gln Thr Ser Leu Asp Glu Gly Ser Leu Ser Ile Glu His
450 455 460
Ile Met Pro Arg Ser Leu Asn Asp Gln Trp Arg Ala Glu Leu Gly Pro
465 470 475 480
Glu Tyr Ala Arg Ile His Glu Thr Trp Ile Asn Arg Ile Gly Asn Leu
485 490 495
Thr Ile Thr Gly Tyr Asn Ser Ala Tyr Ser Asn Ser Ser Tyr Glu Arg
500 505 510
Lys Arg Thr Met Glu Asn Gly Phe Leu Val Ser Pro Tyr Arg Ile Asn
515 520 525
Asn Phe Ile Lys Lys Gln Lys His Trp Ser Glu Glu Gln Leu Ile Glu
530 535 540
Arg Thr Glu Leu Leu Thr Gln Ala Ala Leu Asp Tyr Trp Pro Leu Pro
545 550 555 560
Lys Glu Thr Phe Gln Pro Pro Gln Ala Val Leu Pro Thr Glu Ser Leu
565 570 575
Asp Ser Asp Leu Ser Phe Arg Gly Arg Glu Ile Val Ala Phe Glu Tyr
580 585 590
Glu Asp Tyr Lys Glu Thr Val Thr Ser Trp Ala Asp Met Leu Gln Ser
595 600 605
Val Leu Lys Val Leu Asn Gln Ser Phe Arg Gln Glu Leu Ile Ala Leu
610 615 620
Thr Asn Glu Glu Ile Cys Leu Ala Thr Ser Asn Asn Ser Asn Ser Ser
625 630 635 640
Leu Arg Glu Ile Asp His Gly Leu Phe Val Asp Thr Gly Ser Ser Thr
645 650 655
Ser Val Lys Ile Gly Phe Leu Arg Arg Val Phe Thr Gln Leu Gly Leu
660 665 670
Glu Gln Glu Ala Leu Val Phe Thr Leu Arg Pro Leu Ala Asn Asp Val
675 680 685
Glu Pro Arg Asp Asp Glu Leu Glu Val Glu Val Glu Lys Lys Tyr Ser
690 695 700
Asp Leu Thr Lys Phe Ile Pro Gln Leu Glu Glu Ala Glu Asn Leu Glu
705 710 715 720
Gly Ala Asp Thr Glu Val Ile Pro Leu Leu Ser Lys Leu Lys Glu Gln
725 730 735
Leu Thr Ala Phe Ser Pro Glu Asn Pro Gln Ala Ala Leu Gly Gly Leu
740 745 750
Pro Val Pro Glu Phe Leu Lys Gln Asn Val Ile Glu Gln Leu Ser Ala
755 760 765
Glu His Ile Leu Ala Val Leu Thr Gln His Phe Asn Ile Ala Ser Met
770 775 780
Met Gly Asp Asp Tyr Leu Leu Glu Glu Leu Arg Ser Gly Arg Leu Arg
785 790 795 800
Glu Leu Leu Gln Arg Leu Glu Glu Leu Asp Ser
805 810
<210>289
<211>3829
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3799)
<223>RXA02362
<400>289
acaattgttc ccattcgcat atctcgtgtc caccagagtc gctacagtaa cgagaaactt 60
aattatttga tccgattctt ccgttcaaag gcctcattcc gtg act atc tct cgc 115
Val Thr Ile Ser Arg
1 5
cga ctc aaa caa gag cgc agt ttc gct gac gat ctt caa gat ctc aaa 163
Arg Leu Lys Gln Glu Arg Ser Phe Ala Asp Asp Leu Gln Asp Leu Lys
10 15 20
act ctc aat gat caa ctg cgg ttt aca aac gcc aaa ttg caa gct cgc 211
Thr Leu Asn Asp Gln Leu Arg Phe Thr Asn Ala Lys Leu Gln Ala Arg
25 30 35
atc agt ggt att ggc aat gat gga aag aaa atc acg cgc cct acc cca 259
Ile Ser Gly Ile Gly Asn Asp Gly Lys Lys Ile Thr Arg Pro Thr Pro
40 45 50
ctc ctt gcg ctg gat ttt cag ctg acc gtt gaa gaa tac gaa acg atc 307
Leu Leu Ala Leu Asp Phe Gln Leu Thr Val Glu Glu Tyr Glu Thr Ile
55 60 65
att gca atc ttg gtg gaa gca gtt ggc gga aat caa tcc aag cca gcg 355
Ile Ala Ile Leu Val Glu Ala Val Gly Gly Asn Gln Ser Lys Pro Ala
70 75 80 85
att ctt aaa gat ctg ttt ata gaa tat cca ctc gtc ttc ctg gca gcg 403
Ile Leu Lys Asp Leu Phe Ile Glu Tyr Pro Leu Val Phe Leu Ala Ala
90 95 100
ctt tct gga acc gcc atg ctc gat gct caa gaa ggt ttc tgg cct gcg 451
Leu Ser Gly Thr Ala Met Leu Asp Ala Gln Glu Gly Phe Trp Pro Ala
105 110 115
ttc tgg aaa cgc act cag gtg tca gtt cca gag cat gta tac gac gcg 499
Phe Trp Lys Arg Thr Gln Val Ser Val Pro Glu His Val Tyr Asp Ala
120 125 130
atc cgt aaa gaa cta gtt aat agc atc cgc aaa aat ggc cta gaa act 547
Ile Arg Lys Glu Leu Val Asn Ser Ile Arg Lys Asn Gly Leu Glu Thr
135 140 145
ttt tct ctc gct gac ctc aat cga cgc gaa tat gtc gga ctc atc caa 595
Phe Ser Leu Ala Asp Leu Asn Arg Arg Glu Tyr Val Gly Leu Ile Gln
150 155 160 165
ctt cac agt ggc ctt tct gca aaa gac atg ctc gcc ttg gtc aaa ttt 643
Leu His Ser Gly Leu Ser Ala Lys Asp Met Leu Ala Leu Val Lys Phe
170 175 180
atc gat cac act cga gca gaa aac caa gga tgg gat tct ggt gag gac 691
Ile Asp His Thr Arg Ala Glu Asn Gln Gly Trp Asp Ser Gly Glu Asp
185 190 195
ttt gca tca tat gcg aag agt gtc ttc tcc tcc ggg gac aac cta tta 739
Phe Ala Ser Tyr Ala Lys Ser Val Phe Ser Ser Gly Asp Asn Leu Leu
200 205 210
acc acg gag tcg ctc aag caa tta gtc acc cac atc cct gcg cgt tcc 787
Thr Thr Glu Ser Leu Lys Gln Leu Val Thr His Ile Pro Ala Arg Ser
215 220 225
gtc gac ttc atc gcc aga gtc tat gaa cta acc aat tgg tac cgc gac 835
Val Asp Phe Ile Ala Arg Val Tyr Glu Leu Thr Asn Trp Tyr Arg Asp
230 235 240 245
ctc aaa gac ctc aat gaa gta gaa gcc ttc gta ggt act cat ggg ctg 883
Leu Lys Asp Leu Asn Glu Val Glu Ala Phe Val Gly Thr His Gly Leu
250 255 260
ccg gaa ttg tct ttc aaa ttt ctt ctg gag tgt ctg agc ggc gaa gct 931
Pro Glu Leu Ser Phe Lys Phe Leu Leu Glu Cys Leu Ser Gly Glu Ala
265 270 275
gaa caa att gcc gaa aag acg aaa gca gca cca gca agc ctg gaa aac 979
Glu Gln Ile Ala Glu Lys Thr Lys Ala Ala Pro Ala Ser Leu Glu Asn
280 285 290
ctg gaa cct ccg cat ctc tat ctg gat cca cag agt ttt gaa ctc agt 1027
Leu Glu Pro Pro His Leu Tyr Leu Asp Pro Gln Ser Phe Glu Leu Ser
295 300 305
ctt gtt ttc cca gcg atc tct aaa act gca gca ctt cag att cca gca 1075
Leu Val Phe Pro Ala Ile Ser Lys Thr Ala Ala Leu Gln Ile Pro Ala
310 315 320 325
cca gaa tgg aca gtg att tat gac gga aac tcc att aaa gtt cgt ccc 1123
Pro Glu Trp Thr Val Ile Tyr Asp Gly Asn Ser Ile Lys Val Arg Pro
330 335 340
gaa cag gac tgg tcc tac gga ggt ttc gcc gaa tac cgt ttg cct tta 1171
Glu Gln Asp Trp Ser Tyr Gly Gly Phe Ala Glu Tyr Arg Leu Pro Leu
345 350 355
gac aaa ccg ctc tcc agc ttg aga gtc atc act cca aca gag aaa tcc 1219
Asp Lys Pro Leu Ser Ser Leu Arg Val Ile Thr Pro Thr Glu Lys Ser
360 365 370
cta att ctg att gaa gga ttt ggc cac aag aat ccc att atg ttc ttt 1267
Leu Ile Leu Ile Glu Gly Phe Gly His Lys Asn Pro Ile Met Phe Phe
375 380 385
aag aac aac ggt cag cca tat gca aac caa gaa atg ctc agt ggc aac 1315
Lys Asn Asn Gly Gln Pro Tyr Ala Asn Gln Glu Met Leu Ser Gly Asn
390 395 400 405
gct gtc aca gct ata gtc cca gct gca gca atc att cgt gca cgt atg 1363
Ala Val Thr Ala Ile Val Pro Ala Ala Ala Ile Ile Arg Ala Arg Met
410 415 420
cga gct tcc aag act ttc aac tat caa gac ttg ggt ccc ttg tcc gga 1411
Arg Ala Ser Lys Thr Phe Asn Tyr Gln Asp Leu Gly Pro Leu Ser Gly
425 430 435
tgg aac aag tgg gtc att cgt tcg atc cca ctc aaa cga gct gaa tcg 1459
Trp Asn Lys Trp Val Ile Arg Ser Ile Pro Leu Lys Arg Ala Glu Ser
440 445 450
atc aca gtc tcc cac ggt ggc ttc aga aaa gaa ctc cca gtt cga cgc 1507
Ile Thr Val Ser His Gly Gly Phe Arg Lys Glu Leu Pro Val Arg Arg
455 460 465
aaa gtt gat gtt caa tgg att act gag gat ctc acg atc gag aat ctt 1555
Lys Val Asp Val Gln Trp Ile Thr Glu Asp Leu Thr Ile Glu Asn Leu
470 475 480 485
caa ggt ctc gat cat gag ccc gtt ttc cac acg agt ccc cgc atc gaa 1603
Gln Gly Leu Asp His Glu Pro Val Phe His Thr Ser Pro Arg Ile Glu
490 495 500
ttc ccc acc tct gga tca aac tgg gta att cag tat tca cag att ctt 1651
Phe Pro Thr Ser Gly Ser Asn Trp Val Ile Gln Tyr Ser Gln Ile Leu
505 510 515
cca gat ggc agc ctc atc gaa atg gaa gat tac cca gtc gaa cct gaa 1699
Pro Asp Gly Ser Leu Ile Glu Met Glu Asp Tyr Pro Val Glu Pro Glu
520 525 530
aac ttc gga tac gaa cta gac ctc ttc gaa gaa tcc gac gac cct tgg 1747
Asn Phe Gly Tyr Glu Leu Asp Leu Phe Glu Glu Ser Asp Asp Pro Trp
535 540 545
gtc ggg caa ttt tta gta act ctg ctc aag gat gaa aaa gtc tat gaa 1795
Val Gly Gln Phe Leu Val Thr Leu Leu Lys Asp Glu Lys Val Tyr Glu
550 555 560 565
acc cgc aaa ttc aat ctc gcg gaa ggc ctc gat ctt tcc cta aca ttc 1843
Thr Arg Lys Phe Asn Leu Ala Glu Gly Leu Asp Leu Ser Leu Thr Phe
570 575 580
agc gga ggc gga cct gaa aat cga ttt agg tac ccc agc atc aat cag 1891
Ser Gly Gly Gly Pro Glu Asn Arg Phe Arg Tyr Pro Ser Ile Asn Gln
585 590 595
gga caa act ggc tta aca aag act ttc gcc cgt ttt agt tcc aat tct 1939
Gly Gln Thr Gly Leu Thr Lys Thr Phe Ala Arg Phe Ser Ser Asn Ser
600 605 610
gaa aag cac atc agg ttc cca gat gag atc atc ggg ctt gat gca ttc 1987
Glu Lys His Ile Arg Phe Pro Asp Glu Ile Ile Gly Leu Asp Ala Phe
615 620 625
acc tct caa aaa gcg ttt aac atc gca agc ggt gat ttc cct gag gac 2035
Thr Ser Gln Lys Ala Phe Asn Ile Ala Ser Gly Asp Phe Pro Glu Asp
630 635 640 645
tac aac ctc gac gtt ttc atc acg cct ccg caa ctt cac tac caa gta 2083
Tyr Asn Leu Asp Val Phe Ile Thr Pro Pro Gln Leu His Tyr Gln Val
650 655 660
cct gtc aca cac agc caa aca aag tgg gaa agc aca aag acg aca cta 2131
Pro Val Thr His Ser Gln Thr Lys Trp Glu Ser Thr Lys Thr Thr Leu
665 670 675
gat ttc aat gac ttt gcc gat gga aac ctc cag atc aga ttc cct aat 2179
Asp Phe Asn Asp Phe Ala Asp Gly Asn Leu Gln Ile Arg Phe Pro Asn
680 685 690
gaa gtc tat gat cca aac ttg aaa atc att aaa atg gtg gca tac aag 2227
Glu Val Tyr Asp Pro Asn Leu Lys Ile Ile Lys Met Val Ala Tyr Lys
695 700 705
aaa cct gag tcc agt gag cct aaa tac tta agc aaa att ggt tca agc 2275
Lys Pro Glu Ser Ser Glu Pro Lys Tyr Leu Ser Lys Ile Gly Ser Ser
710 715 720 725
aaa gtg tgg tct atc cct atg gat cgc atc aag gaa ctc atg gat gat 2323
Lys Val Trp Ser Ile Pro Met Asp Arg Ile Lys Glu Leu Met Asp Asp
730 735 740
gat gcc caa ttc ctt ttg atc gcg gag tgg ttc gct gaa agt aaa gac 2371
Asp Ala Gln Phe Leu Leu Ile Ala Glu Trp Phe Ala Glu Ser Lys Asp
745 750 755
cag cac cga gag aag atc att agc gaa gct aag cga act gga aaa atc 2419
Gln His Arg Glu Lys Ile Ile Ser Glu Ala Lys Arg Thr Gly Lys Ile
760 765 770
tcc aat gca gcg ctt aag agt gct cgt cct caa cct caa gca agt tcc 2467
Ser Asn Ala Ala Leu Lys Ser Ala Arg Pro Gln Pro Gln Ala Ser Ser
775 780 785
cac att gca aca att gag aaa aag ccc cta cta gct gcg gct gaa att 2515
His Ile Ala Thr Ile Glu Lys Lys Pro Leu Leu Ala Ala Ala Glu Ile
790 795 800 805
aag ctt tct acc gtg gag ttg gaa ctt ggt cgg cac act tct aag aga 2563
Lys Leu Ser Thr Val Glu Leu Glu Leu Gly Arg His Thr Ser Lys Arg
810 815 820
ctg gaa ggc tgg gca tgg tct gcg ctc aac ccg ctt gat cca cca atc 2611
Leu Glu Gly Trp Ala Trp Ser Ala Leu Asn Pro Leu Asp Pro Pro Ile
825 830 835
aaa gtc gat ttc caa gga acc tca ggc tca ctt cca gac acc cac ttc 2659
Lys Val Asp Phe Gln Gly Thr Ser Gly Ser Leu Pro Asp Thr His Phe
840 845 850
gtc gtt ggc cct tta atc gtg gaa gtg aga gaa aaa gag ttt ctc tcc 2707
Val Val Gly Pro Leu Ile Val Glu Val Arg Glu Lys Glu Phe Leu Ser
855 860 865
caa tgg cag cca aaa gtt ccc tca gtt aaa gcc gtg gtt gca aat gat 2755
Gln Trp Gln Pro Lys Val Pro Ser Val Lys Ala Val Val Ala Asn Asp
870 875 880 885
ccc tca ttt gaa ttg gac cct caa ttt gat cct ttc ctc aca cac cga 2803
Pro Ser Phe Glu Leu Asp Pro Gln Phe Asp Pro Phe Leu Thr His Arg
890 895 900
tgg atg ttc gct cca cga agt ggg aag gtc tta ctc cca caa gaa atc 2851
Trp Met Phe Ala Pro Arg Ser Gly Lys Val Leu Leu Pro Gln Glu Ile
905 910 915
cgc aca gtg tgg gac gcc cga ttc aat atg cgc cat gtc tta gcg cag 2899
Arg Thr Val Trp Asp Ala Arg Phe Asn Met Arg His Val Leu Ala Gln
920 925 930
cgt gaa aac ctt cat gtg aaa tcg att caa gat ttt gac gat gcc acc 2947
Arg Glu Asn Leu His Val Lys Ser Ile Gln Asp Phe Asp Asp Ala Thr
935 940 945
agt acc tat ctc acc agt gat cct cgg gtg gca tta gat gaa ttg gat 2995
Ser Thr Tyr Leu Thr Ser Asp Pro Arg Val Ala Leu Asp Glu Leu Asp
950 955 960 965
aag agc tca att ccg tct aat tcc cac ttt gaa tca ttc atc cga tcc 3043
Lys Ser Ser Ile Pro Ser Asn Ser His Phe Glu Ser Phe Ile Arg Ser
970 975 980
gga tta gct gag ctt tct ttc gaa gtt gac gac aca gcc gga gat atc 3091
Gly Leu Ala Glu Leu Ser Phe Glu Val Asp Asp Thr Ala Gly Asp Ile
985 990 995
cat cgc gtt ccc tgg atc ggc ctg atc cag gaa atg aac gac ctc aga 3139
His Arg Val Pro Trp Ile Gly Leu Ile Gln Glu Met Asn Asp Leu Arg
1000 1005 1010
att ctg cag ata caa ggc tat gaa aca gaa gaa cga gcc atc gaa cgc 3187
Ile Leu Gln Ile Gln Gly Tyr Glu Thr Glu Glu Arg Ala Ile Glu Arg
1015 1020 1025
cga aat tcg cag agc tac atc cgt gag ata gga ggc agt gaa ttg tgg 3235
Arg Asn Ser Gln Ser Tyr Ile Arg Glu Ile Gly Gly Ser Glu Leu Trp
1030 1035 1040 1045
aat atc cta aaa gga aat tca gag gga ttg tct ctt gct caa aaa tgc 3283
Asn Ile Leu Lys Gly Asn Ser Glu Gly Leu Ser Leu Ala Gln Lys Cys
1050 1055 1060
gca cca caa gcc act gag att aat gtg att cgt aat tca ggc ttg gaa 3331
Ala Pro Gln Ala Thr Glu Ile Asn Val Ile Arg Asn Ser Gly Leu Glu
1065 1070 1075
gct atg cgc aat ggg ctg ggc gcc gat cag ttc agc gcc gag ttt att 3379
Ala Met Arg Asn Gly Leu Gly Ala Asp Gln Phe Ser Ala Glu Phe Ile
1080 1085 1090
tca gca gac tca cgc cta cga gct cag ctt gaa tgg ttg gaa aac cgc 3427
Ser Ala Asp Ser Arg Leu Arg Ala Gln Leu Glu Trp Leu Glu Asn Arg
1095 1100 1105
cga gag ctc aat gat ctc ggc cag ctc cca acg ctc ttc gat ttc gcc 3475
Arg Glu Leu Asn Asp Leu Gly Gln Leu Pro Thr Leu Phe Asp Phe Ala
1110 1115 1120 1125
gag aaa tac gag tac ctc atc gat cac tta ggt gat gat cgc atc aag 3523
Glu Lys Tyr Glu Tyr Leu Ile Asp His Leu Gly Asp Asp Arg Ile Lys
1130 1135 1140
gtc act gca cgt gag ctg tct act ctt gcg tcg gaa cac cgt cgc ggc 3571
Val Thr Ala Arg Glu Leu Ser Thr Leu Ala Ser Glu His Arg Arg Gly
1145 1150 1155
aac gct gaa aac tgg ctt tat gca cca tat gtg tca ttc att tac agc 3619
Asn Ala Glu Asn Trp Leu Tyr Ala Pro Tyr Val Ser Phe Ile Tyr Ser
1160 1165 1170
ttg ctt aac cga atg atc gct cat gaa gta ata cgt ccg atc gct cag 3667
Leu Leu Asn Arg Met Ile Ala His Glu Val Ile Arg Pro Ile Ala Gln
1175 1180 1185
atc aat tac tca cgg cac gat tgg gca aac gct gct cgg ctg att cct 3715
Ile Asn Tyr Ser Arg His Asp Trp Ala Asn Ala Ala Arg Leu Ile Pro
1190 1195 1200 1205
cgt ctc aca gga ttt gac ctg gtg agt gcc gaa gcg aaa gtg ctc agc 3763
Arg Leu Thr Gly Phe Asp Leu Val Ser Ala Glu Ala Lys Val Leu Ser
1210 1215 1220
gca ata aac aac aac aat ata atc cca act gca att taaggatcac 3809
Ala Ile Asn Asn Asn Asn Ile Ile Pro Thr Ala Ile
1225 1230
tatgtccaac gcacctaaaa 3829
<210>290
<211>1233
<212>PRT
<213>谷氨酸棒状杆菌
<400>290
Val Thr Ile Ser Arg Arg Leu Lys Gln Glu Arg Ser Phe Ala Asp Asp
1 5 10 15
Leu Gln Asp Leu Lys Thr Leu Asn Asp Gln Leu Arg Phe Thr Asn Ala
20 25 30
Lys Leu Gln Ala Arg Ile Ser Gly Ile Gly Asn Asp Gly Lys Lys Ile
35 40 45
Thr Arg Pro Thr Pro Leu Leu Ala Leu Asp Phe Gln Leu Thr Val Glu
50 55 60
Glu Tyr Glu Thr Ile Ile Ala Ile Leu Val Glu Ala Val Gly Gly Asn
65 70 75 80
Gln Ser Lys Pro Ala Ile Leu Lys Asp Leu Phe Ile Glu Tyr Pro Leu
85 90 95
Val Phe Leu Ala Ala Leu Ser Gly Thr Ala Met Leu Asp Ala Gln Glu
100 105 110
Gly Phe Trp Pro Ala Phe Trp Lys Arg Thr Gln Val Ser Val Pro Glu
115 120 125
His Val Tyr Asp Ala Ile Arg Lys Glu Leu Val Asn Ser Ile Arg Lys
130 135 140
Asn Gly Leu Glu Thr Phe Ser Leu Ala Asp Leu Asn Arg Arg Glu Tyr
145 150 155 160
Val Gly Leu Ile Gln Leu His Ser Gly Leu Ser Ala Lys Asp Met Leu
165 170 175
Ala Leu Val Lys Phe Ile Asp His Thr Arg Ala Glu Asn Gln Gly Trp
180 185 190
Asp Ser Gly Glu Asp Phe Ala Ser Tyr Ala Lys Ser Val Phe Ser Ser
195 200 205
Gly Asp Asn Leu Leu Thr Thr Glu Ser Leu Lys Gln Leu Val Thr His
210 215 220
Ile Pro Ala Arg Ser Val Asp Phe Ile Ala Arg Val Tyr Glu Leu Thr
225 230 235 240
Asn Trp Tyr Arg Asp Leu Lys Asp Leu Asn Glu Val Glu Ala Phe Val
245 250 255
Gly Thr His Gly Leu Pro Glu Leu Ser Phe Lys Phe Leu Leu Glu Cys
260 265 270
Leu Ser Gly Glu Ala Glu Gln Ile Ala Glu Lys Thr Lys Ala Ala Pro
275 280 285
Ala Ser Leu Glu Asn Leu Glu Pro Pro His Leu Tyr Leu Asp Pro Gln
290 295 300
Ser Phe Glu Leu Ser Leu Val Phe Pro Ala Ile Ser Lys Thr Ala Ala
305 310 315 320
Leu Gln Ile Pro Ala Pro Glu Trp Thr Val Ile Tyr Asp Gly Asn Ser
325 330 335
Ile Lys Val Arg Pro Glu Gln Asp Trp Ser Tyr Gly Gly Phe Ala Glu
340 345 350
Tyr Arg Leu Pro Leu Asp Lys Pro Leu Ser Ser Leu Arg Val Ile Thr
355 360 365
Pro Thr Glu Lys Ser Leu Ile Leu Ile Glu Gly Phe Gly His Lys Asn
370 375 380
Pro Ile Met Phe Phe Lys Asn Asn Gly Gln Pro Tyr Ala Asn Gln Glu
385 390 395 400
Met Leu Ser Gly Asn Ala Val Thr Ala Ile Val Pro Ala Ala Ala Ile
405 410 415
Ile Arg Ala Arg Met Arg Ala Ser Lys Thr Phe Asn Tyr Gln Asp Leu
420 425 430
Gly Pro Leu Ser Gly Trp Asn Lys Trp Val Ile Arg Ser Ile Pro Leu
435 440 445
Lys Arg Ala Glu Ser Ile Thr Val Ser His Gly Gly Phe Arg Lys Glu
450 455 460
Leu Pro Val Arg Arg Lys Val Asp Val Gln Trp Ile Thr Glu Asp Leu
465 470 475 480
Thr Ile Glu Asn Leu Gln Gly Leu Asp His Glu Pro Val Phe His Thr
485 490 495
Ser Pro Arg Ile Glu Phe Pro Thr Ser Gly Ser Asn Trp Val Ile Gln
500 505 510
Tyr Ser Gln Ile Leu Pro Asp Gly Ser Leu Ile Glu Met Glu Asp Tyr
515 520 525
Pro Val Glu Pro Glu Asn Phe Gly Tyr Glu Leu Asp Leu Phe Glu Glu
530 535 540
Ser Asp Asp Pro Trp Val Gly Gln Phe Leu Val Thr Leu Leu Lys Asp
545 550 555 560
Glu Lys Val Tyr Glu Thr Arg Lys Phe Asn Leu Ala Glu Gly Leu Asp
565 570 575
Leu Ser Leu Thr Phe Ser Gly Gly Gly Pro Glu Asn Arg Phe Arg Tyr
580 585 590
Pro Ser Ile Asn Gln Gly Gln Thr Gly Leu Thr Lys Thr Phe Ala Arg
595 600 605
Phe Ser Ser Asn Ser Glu Lys His Ile Arg Phe Pro Asp Glu Ile Ile
610 615 620
Gly Leu Asp Ala Phe Thr Ser Gln Lys Ala Phe Asn Ile Ala Ser Gly
625 630 635 640
Asp Phe Pro Glu Asp Tyr Asn Leu Asp Val Phe Ile Thr Pro Pro Gln
645 650 655
Leu His Tyr Gln Val Pro Val Thr His Ser Gln Thr Lys Trp Glu Ser
660 665 670
Thr Lys Thr Thr Leu Asp Phe Asn Asp Phe Ala Asp Gly Asn Leu Gln
675 680 685
Ile Arg Phe Pro Asn Glu Val Tyr Asp Pro Asn Leu Lys Ile Ile Lys
690 695 700
Met Val Ala Tyr Lys Lys Pro Glu Ser Ser Glu Pro Lys Tyr Leu Ser
705 710 715 720
Lys Ile Gly Ser Ser Lys Val Trp Ser Ile Pro Met Asp Arg Ile Lys
725 730 735
Glu Leu Met Asp Asp Asp Ala Gln Phe Leu Leu Ile Ala Glu Trp Phe
740 745 750
Ala Glu Ser Lys Asp Gln His Arg Glu Lys Ile Ile Ser Glu Ala Lys
755 760 765
Arg Thr Gly Lys Ile Ser Asn Ala Ala Leu Lys Ser Ala Arg Pro Gln
770 775 780
Pro Gln Ala Ser Ser His Ile Ala Thr Ile Glu Lys Lys Pro Leu Leu
785 790 795 800
Ala Ala Ala Glu Ile Lys Leu Ser Thr Val Glu Leu Glu Leu Gly Arg
805 810 815
His Thr Ser Lys Arg Leu Glu Gly Trp Ala Trp Ser Ala Leu Asn Pro
820 825 830
Leu Asp Pro Pro Ile Lys Val Asp Phe Gln Gly Thr Ser Gly Ser Leu
835 840 845
Pro Asp Thr His Phe Val Val Gly Pro Leu Ile Val Glu Val Arg Glu
850 855 860
Lys Glu Phe Leu Ser Gln Trp Gln Pro Lys Val Pro Ser Val Lys Ala
865 870 875 880
Val Val Ala Asn Asp Pro Ser Phe Glu Leu Asp Pro Gln Phe Asp Pro
885 890 895
Phe Leu Thr His Arg Trp Met Phe Ala Pro Arg Ser Gly Lys Val Leu
900 905 910
Leu Pro Gln Glu Ile Arg Thr Val Trp Asp Ala Arg Phe Asn Met Arg
915 920 925
His Val Leu Ala Gln Arg Glu Asn Leu His Val Lys Ser Ile Gln Asp
930 935 940
Phe Asp Asp Ala Thr Ser Thr Tyr Leu Thr Ser Asp Pro Arg Val Ala
945 950 955 960
Leu Asp Glu Leu Asp Lys Ser Ser Ile Pro Ser Asn Ser His Phe Glu
965 970 975
Ser Phe Ile Arg Ser Gly Leu Ala Glu Leu Ser Phe Glu Val Asp Asp
980 985 990
Thr Ala Gly Asp Ile His Arg Val Pro Trp Ile Gly Leu Ile Gln Glu
995 1000 1005
Met Asn Asp Leu Arg Ile Leu Gln Ile Gln Gly Tyr Glu Thr Glu Glu
1010 1015 1020
Arg Ala Ile Glu Arg Arg Asn Ser Gln Ser Tyr Ile Arg Glu Ile Gly
1025 1030 1035 1040
Gly Ser Glu Leu Trp Asn Ile Leu Lys Gly Asn Ser Glu Gly Leu Ser
1045 1050 1055
Leu Ala Gln Lys Cys Ala Pro Gln Ala Thr Glu Ile Asn Val Ile Arg
1060 1065 1070
Asn Ser Gly Leu Glu Ala Met Arg Asn Gly Leu Gly Ala Asp Gln Phe
1075 1080 1085
Ser Ala Glu Phe Ile Ser Ala Asp Ser Arg Leu Arg Ala Gln Leu Glu
1090 1095 1100
Trp Leu Glu Asn Arg Arg Glu Leu Asn Asp Leu Gly Gln Leu Pro Thr
1105 1110 1115 1120
Leu Phe Asp Phe Ala Glu Lys Tyr Glu Tyr Leu Ile Asp His Leu Gly
1125 1130 1135
Asp Asp Arg Ile Lys Val Thr Ala Arg Glu Leu Ser Thr Leu Ala Ser
1140 1145 1150
Glu His Arg Arg Gly Asn Ala Glu Asn Trp Leu Tyr Ala Pro Tyr Val
1155 1160 1165
Ser Phe Ile Tyr Ser Leu Leu Asn Arg Met Ile Ala His Glu Val Ile
1170 1175 1180
Arg Pro Ile Ala Gln Ile Asn Tyr Ser Arg His Asp Trp Ala Asn Ala
1185 1190 1195 1200
Ala Arg Leu Ile Pro Arg Leu Thr Gly Phe Asp Leu Val Ser Ala Glu
1205 1210 1215
Ala Lys Val Leu Ser Ala Ile Asn Asn Asn Asn Ile Ile Pro Thr Ala
1220 1225 1230
Ile
<210>291
<211>793
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(763)
<223>RXA02367
<400>291
ggatccagga aaagtattca tcgaagtttc cctgaaaggg atctcagcca tcaaggcact 60
tgaatccgga cgctaagttg ttgatacatc aacttatttc ttg gct ata ctt gag 115
Leu Ala Ile Leu Glu
1 5
gtc acg ttt cct cag ctc agg aga agt tcg gag aag tcc atg tca aac 163
Val Thr Phe Pro Gln Leu Arg Arg Ser Ser Glu Lys Ser Met Ser Asn
10 15 20
gca gaa att aat ccc gtt gaa tat gaa atc aac aac cac gcc cct ggt 211
Ala Glu Ile Asn Pro Val Glu Tyr Glu Ile Asn Asn His Ala Pro Gly
25 30 35
acc gcg cta aac cca cag tgc gaa gac ggc gcc aac gta gaa atc atc 259
Thr Ala Leu Asn Pro Gln Cys Glu Asp Gly Ala Asn Val Glu Ile Ile
40 45 50
acc tcc cgt gaa gtc ccc ctc ggc gga cca cgc gcc atg acc gtg cac 307
Thr Ser Arg Glu Val Pro Leu Gly Gly Pro Arg Ala Met Thr Val His
55 60 65
cgc aca ctc ccc caa cgc cag cgc tcc ctc atc ggt gcc tgg tgt ttt 355
Arg Thr Leu Pro Gln Arg Gln Arg Ser Leu Ile Gly Ala Trp Cys Phe
70 75 80 85
gtg gat cat tac ggc ccc gat gat gtc tca cta acc ggt ggc atg gat 403
Val Asp His Tyr Gly Pro Asp Asp Val Ser Leu Thr Gly Gly Met Asp
90 95 100
atg gcc tcc cac ccg cac acc gga ttg caa aca gtc acg tgg ctt ttt 451
Met Ala Ser His Pro His Thr Gly Leu Gln Thr Val Thr Trp Leu Phe
105 110 115
gaa ggc gaa gtc acc cac cat gat tcc ggc gga aat cac gca gtc gtg 499
Glu Gly Glu Val Thr His His Asp Ser Gly Gly Asn His Ala Val Val
120 125 130
ctt cct ggc gaa gtc aac ctc atg acc gcc ggc gca ggc atc tgt cac 547
Leu Pro Gly Glu Val Asn Leu Met Thr Ala Gly Ala Gly Ile Cys His
135 140 145
acc gaa gtt tcc gcc acc tcc acc acg att ctg cat ggt ctg cag ttg 595
Thr Glu Val Ser Ala Thr Ser Thr Thr Ile Leu His Gly Leu Gln Leu
150 155 160 165
tgg aca gtt ctt ccc gac aaa gac cgc gaa gga cca cgc cgc ttt gat 643
Trp Thr Val Leu Pro Asp Lys Asp Arg Glu Gly Pro Arg Arg Phe Asp
170 175 180
cac tac gct cca gaa gaa atc aca ctc gaa ggc gga agt gca cgc gtt 691
His Tyr Ala Pro Glu Glu Ile Thr Leu Glu Gly Gly Ser Ala Arg Val
185 190 195
ttc cta ggt tcg cta ttt ggt caa acc tcc cct gtt cat acg ttt act 739
Phe Leu Gly Ser Leu Phe Gly Gln Thr Ser Pro Val His Thr Phe Thr
200 205 210
ggc tct tcc tgt ttt aga gtg cat tgatcttatg gaccaactgc cctgaatgga 793
Gly Ser Ser Cys Phe Arg Val His
215 220
<210>292
<211>221
<212>PRT
<213>谷氨酸棒状杆菌
<400>292
Leu Ala Ile Leu Glu Val Thr Phe Pro Gln Leu Arg Arg Ser Ser Glu
1 5 10 15
Lys Ser Met Ser Asn Ala Glu Ile Asn Pro Val Glu Tyr Glu Ile Asn
20 25 30
Asn His Ala Pro Gly Thr Ala Leu Asn Pro Gln Cys Glu Asp Gly Ala
35 40 45
Asn Val Glu Ile Ile Thr Ser Arg Glu Val Pro Leu Gly Gly Pro Arg
50 55 60
Ala Met Thr Val His Arg Thr Leu Pro Gln Arg Gln Arg Ser Leu Ile
65 70 75 80
Gly Ala Trp Cys Phe Val Asp His Tyr Gly Pro Asp Asp Val Ser Leu
85 90 95
Thr Gly Gly Met Asp Met Ala Ser His Pro His Thr Gly Leu Gln Thr
100 105 110
Val Thr Trp Leu Phe Glu Gly Glu Val Thr His His Asp Ser Gly Gly
115 120 125
Asn His Ala Val Val Leu Pro Gly Glu Val Asn Leu Met Thr Ala Gly
130 135 140
Ala Gly Ile Cys His Thr Glu Val Ser Ala Thr Ser Thr Thr Ile Leu
145 150 155 160
His Gly Leu Gln Leu Trp Thr Val Leu Pro Asp Lys Asp Arg Glu Gly
165 170 175
Pro Arg Arg Phe Asp His Tyr Ala Pro Glu Glu Ile Thr Leu Glu Gly
180 185 190
Gly Ser Ala Arg Val Phe Leu Gly Ser Leu Phe Gly Gln Thr Ser Pro
195 200 205
Val His Thr Phe Thr Gly Ser Ser Cys Phe Arg Val His
210 215 220
<210>293
<211>1123
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1093)
<223>RXA02387
<400>293
cagagaagga atccctgtgc tgtatccata ccgcgatggg gaggtcagcc ttcatattga 60
tggcacccag catgcagcgg acgggagatt ttaacgacac ttg tgg cac gat ggt 115
Leu Trp His Asp Gly
1 5
cac gtg gct tca gca cct att tca gtt caa cca gcg gtc cat ctt gtt 163
His Val Ala Ser Ala Pro Ile Ser Val Gln Pro Ala Val His Leu Val
10 15 20
ctc ggc gag gat gag ttc ctt gcc gaa cgc gcg cgt atc aac atc gtg 211
Leu Gly Glu Asp Glu Phe Leu Ala Glu Arg Ala Arg Ile Asn Ile Val
25 30 35
cat gac atc cgt tca tcg atg gct agc ccc gat tcg ctt cag gtc acc 259
His Asp Ile Arg Ser Ser Met Ala Ser Pro Asp Ser Leu Gln Val Thr
40 45 50
acc ttg aag gcc agc gaa att act cag ggt gaa ttg ttg gat gcg tta 307
Thr Leu Lys Ala Ser Glu Ile Thr Gln Gly Glu Leu Leu Asp Ala Leu
55 60 65
agc cca tcc ttg ttt gct gaa gac cgt gtg gtg gtg ctt acc aac atg 355
Ser Pro Ser Leu Phe Ala Glu Asp Arg Val Val Val Leu Thr Asn Met
70 75 80 85
gat caa gca ggc caa gat gct gta gat cta gcc tta tcc gca gca gtt 403
Asp Gln Ala Gly Gln Asp Ala Val Asp Leu Ala Leu Ser Ala Ala Val
90 95 100
gat ccc agc cct ggt acc tac ttg atc gtc atg cac tct ggc ggt gga 451
Asp Pro Ser Pro Gly Thr Tyr Leu Ile Val Met His Ser Gly Gly Gly
105 110 115
cgc tct aag tcg atg gtg aag aag ctg gaa aaa gtc gcg gtg gtg cac 499
Arg Ser Lys Ser Met Val Lys Lys Leu Glu Lys Val Ala Val Val His
120 125 130
gat gcc gca aag ctg aaa gac cgg gat cgt cca ggt tgg gta aaa caa 547
Asp Ala Ala Lys Leu Lys Asp Arg Asp Arg Pro Gly Trp Val Lys Gln
135 140 145
gag ttc aaa aac cac aaa gtc cag gtc acc cca gat gtc att cat gct 595
Glu Phe Lys Asn His Lys Val Gln Val Thr Pro Asp Val Ile His Ala
150 155 160 165
ctt tta gag ggc gtg ggt tca gat ctt aga gag ctg gcg tcc gcc gta 643
Leu Leu Glu Gly Val Gly Ser Asp Leu Arg Glu Leu Ala Ser Ala Val
170 175 180
tcc caa ttg gtt gag gac acc caa ggc aac gtg acg gtg gaa aaa gtc 691
Ser Gln Leu Val Glu Asp Thr Gln Gly Asn Val Thr Val Glu Lys Val
185 190 195
cgt gcc tat tac gtg ggt gtt gct gag gta tcg ggt ttc gac atc gcc 739
Arg Ala Tyr Tyr Val Gly Val Ala Glu Val Ser Gly Phe Asp Ile Ala
200 205 210
gat tct gca tgc gcc ggt caa atg tca aag gcc gtg gcc agc acc aga 787
Asp Ser Ala Cys Ala Gly Gln Met Ser Lys Ala Val Ala Ser Thr Arg
215 220 225
cgt gcc ctt caa ttg ggt acc agc ccg gtt gca ttg gca gct gcg ttg 835
Arg Ala Leu Gln Leu Gly Thr Ser Pro Val Ala Leu Ala Ala Ala Leu
230 235 240 245
agt atg aaa gtt ggc cag atc gcc agg ctg tat tcc acc agg gga cgc 883
Ser Met Lys Val Gly Gln Ile Ala Arg Leu Tyr Ser Thr Arg Gly Arg
250 255 260
atc aac ggt ttt gag ctg gcc aaa gaa ttg ggc atg ccg ccg ttc gtg 931
Ile Asn Gly Phe Glu Leu Ala Lys Glu Leu Gly Met Pro Pro Phe Val
265 270 275
gtg gag aag act gcg aaa gtg gcc cga aac tgg tcg gga gat gcg gtc 979
Val Glu Lys Thr Ala Lys Val Ala Arg Asn Trp Ser Gly Asp Ala Val
280 285 290
agc gag gcc gtg att ttg atg gcc gat ctg gat gcc gct gta aaa gga 1027
Ser Glu Ala Val Ile Leu Met Ala Asp Leu Asp Ala Ala Val Lys Gly
295 300 305
caa agt ggc gat cct gaa ttt gcc atc gaa tct gcc gtg aga aga gtt 1075
Gln Ser Gly Asp Pro Glu Phe Ala Ile Glu Ser Ala Val Arg Arg Val
310 315 320 325
gca gag ctg gcg agg cgg taacgctgaa cggcggcggg taagatattt 1123
Ala Glu Leu Ala Arg Arg
330
<210>294
<211>331
<212>PRT
<213>谷氨酸棒状杆菌
<400>294
Leu Trp His Asp Gly His Val Ala Ser Ala Pro Ile Ser Val Gln Pro
l 5 10 15
Ala Val His Leu Val Leu Gly Glu Asp Glu Phe Leu Ala Glu Arg Ala
20 25 30
Arg Ile Asn Ile Val His Asp Ile Arg Ser Ser Met Ala Ser Pro Asp
35 40 45
Ser Leu Gln Val Thr Thr Leu Lys Ala Ser Glu Ile Thr Gln Gly Glu
50 55 60
Leu Leu Asp Ala Leu Ser Pro Ser Leu Phe Ala Glu Asp Arg Val Val
65 70 75 80
Val Leu Thr Asn Met Asp Gln Ala Gly Gln Asp Ala Val Asp Leu Ala
85 90 95
Leu Ser Ala Ala Val Asp Pro Ser Pro Gly Thr Tyr Leu Ile Val Met
100 105 110
His Ser Gly Gly Gly Arg Ser Lys Ser Met Val Lys Lys Leu Glu Lys
115 120 125
Val Ala Val Val His Asp Ala Ala Lys Leu Lys Asp Arg Asp Arg Pro
130 135 140
Gly Trp Val Lys Gln Glu Phe Lys Asn His Lys Val Gln Val Thr Pro
145 150 155 160
Asp Val Ile His Ala Leu Leu Glu Gly Val Gly Ser Asp Leu Arg Glu
165 170 175
Leu Ala Ser Ala Val Ser Gln Leu Val Glu Asp Thr Gln Gly Asn Val
180 185 190
Thr Val Glu Lys Val Arg Ala Tyr Tyr Val Gly Val Ala Glu Val Ser
195 200 205
Gly Phe Asp Ile Ala Asp Ser Ala Cys Ala Gly Gln Met Ser Lys Ala
210 215 220
Val Ala Ser Thr Arg Arg Ala Leu Gln Leu Gly Thr Ser Pro Val Ala
225 230 235 240
Leu Ala Ala Ala Leu Ser Met Lys Val Gly Gln Ile Ala Arg Leu Tyr
245 250 255
Ser Thr Arg Gly Arg Ile Asn Gly Phe Glu Leu Ala Lys Glu Leu Gly
260 265 270
Met Pro Pro Phe Val Val Glu Lys Thr Ala Lys Val Ala Arg Asn Trp
275 280 285
Ser Gly Asp Ala Val Ser Glu Ala Val Ile Leu Met Ala Asp Leu Asp
290 295 300
Ala Ala Val Lys Gly Gln Ser Gly Asp Pro Glu Phe Ala Ile Glu Ser
305 310 315 320
Ala Val Arg Arg Val Ala Glu Leu Ala Arg Arg
325 330
<210>295
<211>799
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(769)
<223>RXA02390
<400>295
gctggtggtg ctgacccata cgctggaact ccaactgctg ttgataccgc caagatgttt 60
ggccgcgagg atctcgtagc tcgcttcgag tcataggccg gtg gag tgg acc gct 115
Val Glu Trp Thr Ala
1 5
ttt ggc acc ctg att ctg ctc aat ttg gtg ggc agt tta tcc ccg ggg 163
Phe Gly Thr Leu Ile Leu Leu Asn Leu Val Gly Ser Leu Ser Pro Gly
10 15 20
cct gat acc ttt ttc ctc ctc cgc tta gcc acc cgc tcc aga gcg cac 211
Pro Asp Thr Phe Phe Leu Leu Arg Leu Ala Thr Arg Ser Arg Ala His
25 30 35
gcg atc gct ggc gtc gcc ggc atc gtc acc gga ctc acg gtg tgg gtg 259
Ala Ile Ala Gly Val Ala Gly Ile Val Thr Gly Leu Thr Val Trp Val
40 45 50
acg ctg acg gtc gtg gga gca gcg gcg ctg ctc acc act tat ccg tcg 307
Thr Leu Thr Val Val Gly Ala Ala Ala Leu Leu Thr Thr Tyr Pro Ser
55 60 65
att ctc gga atc atc cag ctc gtc ggc ggc acg tac cta agc ttc att 355
Ile Leu Gly Ile Ile Gln Leu Val Gly Gly Thr Tyr Leu Ser Phe Ile
70 75 80 85
ggg tac aag ttg ctg cgc tcg gcg tcg aga gag ctt atc gac gcc cgc 403
Gly Tyr Lys Leu Leu Arg Ser Ala Ser Arg Glu Leu Ile Asp Ala Arg
90 95 100
cag ttc cgt ttc aac gcc gat gcc cga cct atc ccg gat gcg gta gaa 451
Gln Phe Arg Phe Asn Ala Asp Ala Arg Pro Ile Pro Asp Ala Val Glu
105 110 115
gca ctg gga acc cgc act cag gta tat cga caa ggt ttg gcc acc aac 499
Ala Leu Gly Thr Arg Thr Gln Val Tyr Arg Gln Gly Leu Ala Thr Asn
120 125 130
ctg tca aac cct aaa gtt gtc atg tac ttc gcg gca att ctg gct ccg 547
Leu Ser Asn Pro Lys Val Val Met Tyr Phe Ala Ala Ile Leu Ala Pro
135 140 145
ttg atg cca gcg cac cca tca ccg gtg ctg gcg ttc tct atc atc gtg 595
Leu Met Pro Ala His Pro Ser Pro Val Leu Ala Phe Ser Ile Ile Val
150 155 160 165
gcg att tta gtg cag acc ttt gtt acc ttc tct gct gtg tgc ctc att 643
Ala Ile Leu Val Gln Thr Phe Val Thr Phe Ser Ala Val Cys Leu Ile
170 175 180
gtc tct acg gag cgt gtg cgc aaa gca atg ctg cgt gca ggt ccc tgg 691
Val Ser Thr Glu Arg Val Arg Lys Ala Met Leu Arg Ala Gly Pro Trp
185 190 195
ttt gac ctg ctt gct ggc gtt gtc ttc ctc gtt gtg ggt gtg act ctg 739
Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val Val Gly Val Thr Leu
200 205 210
ctg tat gaa ggc ctg acc ggt tta ctc ggg taaaggcata aaaaatggct 789
Leu Tyr Glu Gly Leu Thr Gly Leu Leu Gly
215 220
tccaatcaaa 799
<210>296
<211>223
<212>PRT
<213>谷氨酸棒状杆菌
<400>296
Val Glu Trp Thr Ala Phe Gly Thr Leu Ile Leu Leu Asn Leu Val Gly
1 5 10 15
Ser Leu Ser Pro Gly Pro Asp Thr Phe Phe Leu Leu Arg Leu Ala Thr
20 25 30
Arg Ser Arg Ala His Ala Ile Ala Gly Val Ala Gly Ile Val Thr Gly
35 40 45
Leu Thr Val Trp Val Thr Leu Thr Val Val Gly Ala Ala Ala Leu Leu
50 55 60
Thr Thr Tyr Pro Ser Ile Leu Gly Ile Ile Gln Leu Val Gly Gly Thr
65 70 75 80
Tyr Leu Ser Phe Ile Gly Tyr Lys Leu Leu Arg Ser Ala Ser Arg Glu
85 90 95
Leu Ile Asp Ala Arg Gln Phe Arg Phe Asn Ala Asp Ala Arg Pro Ile
100 105 110
Pro Asp Ala Val Glu Ala Leu Gly Thr Arg Thr Gln Val Tyr Arg Gln
115 120 125
Gly Leu Ala Thr Asn Leu Ser Asn Pro Lys Val Val Met Tyr Phe Ala
130 135 140
Ala Ile Leu Ala Pro Leu Met Pro Ala His Pro Ser Pro Val Leu Ala
145 150 155 160
Phe Ser Ile Ile Val Ala Ile Leu Val Gln Thr Phe Val Thr Phe Ser
165 170 175
Ala Val Cys Leu Ile Val Ser Thr Glu Arg Val Arg Lys Ala Met Leu
180 185 190
Arg Ala Gly Pro Trp Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val
195 200 205
Val Gly Val Thr Leu Leu Tyr Glu Gly Leu Thr Gly Leu Leu Gly
210 215 220
<210>297
<2ll>2035
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2005)
<223>RXA02395
<400>297
ttgcttataa tacaaaaaca ggaatattta actcgacttc ttaaaaaagc tctatacgta 60
aatatcttgc ccatccagca ccacctcatt gtggtgcaca atg gag agg aca cac 115
Met Glu Arg Thr His
1 5
atg tcc acc aac tct ggc aat aac ttg cct gaa tcc caa gag tca ccc 163
Met Ser Thr Asn Ser Gly Asn Asn Leu Pro Glu Ser Gln Glu Ser Pro
10 15 20
gag gaa cct cat tat cct cac gac acc cac cca ggc cta gtt cct ggc 211
Glu Glu Pro His Tyr Pro His Asp Thr His Pro Gly Leu Val Pro Gly
25 30 35
att tcg gtc gat gct caa cgc aac aag ttc gga ctc gac aaa acc gtt 259
Ile Ser Val Asp Ala Gln Arg Asn Lys Phe Gly Leu Asp Lys Thr Val
40 45 50
ttc ggc gtc acc gca gca tta att ctg gcg ttc atc gcc tgg ggc att 307
Phe Gly Val Thr Ala Ala Leu Ile Leu Ala Phe Ile Ala Trp Gly Ile
55 60 65
tcc agc cct gat tcg gtt tct tca gta tcg tcc acg atg ttc agt tgg 355
Ser Ser Pro Asp Ser Val Ser Ser Val Ser Ser Thr Met Phe Ser Trp
70 75 80 85
gct atg acg aat act ggt tgg ctc ctc aac ttt gtc atg ctg atc ggc 403
Ala Met Thr Asn Thr Gly Trp Leu Leu Asn Phe Val Met Leu Ile Gly
90 95 100
att ggc acc atg ctc tac atc gct ttt tcg cgc tac ggc cgc atc aag 451
Ile Gly Thr Met Leu Tyr Ile Ala Phe Ser Arg Tyr Gly Arg Ile Lys
105 110 115
ctg ggc acg gac gaa gac gaa cca gaa ttc tct cgc ttt tcc tgg att 499
Leu Gly Thr Asp Glu Asp Glu Pro Glu Phe Ser Arg Phe Ser Trp Ile
120 125 130
gcg atg atg ttc ggc gcc ggc atc ggt gtc ggt atc ttc ttc ttc ggt 547
Ala Met Met Phe Gly Ala Gly Ile Gly Val Gly Ile Phe Phe Phe Gly
135 140 145
cct tcc gaa ccg ctg tgg cat tat ctc agt ccc cca cct cac acc gtg 595
Pro Ser Glu Pro Leu Trp His Tyr Leu Ser Pro Pro Pro His Thr Val
150 155 160 165
gaa gga tct aca cct gag tcc ttg cac caa gca ctt gcg cag tcc cac 643
Glu Gly Ser Thr Pro Glu Ser Leu His Gln Ala Leu Ala Gln Ser His
170 175 180
ttc cac tgg ggc tta tcc gcc tgg ggg ctg tat gcc ttg gtc ggc ggc 691
Phe His Trp Gly Leu Ser Ala Trp Gly Leu Tyr Ala Leu Val Gly Gly
185 190 195
gcg ttg gcg tac tcc agt tac cga cgc ggc cgc gta acc ttg atc agc 739
Ala Leu Ala Tyr Ser Ser Tyr Arg Arg Gly Arg Val Thr Leu Ile Ser
200 205 210
tcc acc ttc cgg tca ctt ttc ggt gag aaa acc gaa ggt atc gcg ggt 787
Ser Thr Phe Arg Ser Leu Phe Gly Glu Lys Thr Glu Gly Ile Ala Gly
215 220 225
cgc ctc atc gac atg atg gcg atc atc gcc acg ctg ttt gga acg gct 835
Arg Leu Ile Asp Met Met Ala Ile Ile Ala Thr Leu Phe Gly Thr Ala
230 235 240 245
gca acc ttg ggc ctt tca gca att cag gtt ggt cag ggc gtg cag att 883
Ala Thr Leu Gly Leu Ser Ala Ile Gln Val Gly Gln Gly Val Gln Ile
250 255 260
att tct ggc gct tcg gaa atc acc aac aac atc ttg atc gcc atc atc 931
Ile Ser Gly Ala Ser Glu Ile Thr Asn Asn Ile Leu Ile Ala Ile Ile
265 270 275
gcg atc ttg acc att ggc ttc atc atc tcc tca gta tct ggc gtg tcc 979
Ala Ile Leu Thr Ile Gly Phe Ile Ile Ser Ser Val Ser Gly Val Ser
280 285 290
aag ggc att cgt tat ctt tcc aac ctc aat att tcc ttg acg ctt gga 1027
Lys Gly Ile Arg Tyr Leu Ser Asn Leu Asn Ile Ser Leu Thr Leu Gly
295 300 305
ttg gtc ctg ttt gtg ttc atc acc ggc ccc acc ttg ttc ctg ctc aac 1075
Leu Val Leu Phe Val Phe Ile Thr Gly Pro Thr Leu Phe Leu Leu Asn
310 315 320 325
ttg atc cca tcg agt gtg ttg gaa tat ggc agt gag ttc ttg tca atg 1123
Leu Ile Pro Ser Ser Val Leu Glu Tyr Gly Ser Glu Phe Leu Ser Met
330 335 340
gct ggc aag tct ttg tcg tgg ggt gag gaa acc att gaa ttc caa gct 1171
Ala Gly Lys Ser Leu Ser Trp Gly Glu Glu Thr Ile Glu Phe Gln Ala
345 350 355
ggt tgg acg gcg ttc tac tgg gca tgg tgg atc gca tgg act cca ttc 1219
Gly Trp Thr Ala Phe Tyr Trp Ala Trp Trp Ile Ala Trp Thr Pro Phe
360 365 370
gtg ggt atg ttc atc gcg cgt att tct cgc ggc cgc acc ttg cgt gaa 1267
Val Gly Met Phe Ile Ala Arg Ile Ser Arg Gly Arg Thr Leu Arg Glu
375 380 385
ttc gcg ctc atc acc atg gct atc ccc tcc ttc att ttg atc ctg gcg 1315
Phe Ala Leu Ile Thr Met Ala Ile Pro Ser Phe Ile Leu Ile Leu Ala
390 395 400 405
ttc acc att ttc ggt gga act gcc atc acg atg aac cgc gag aac gta 1363
Phe Thr Ile Phe Gly Gly Thr Ala Ile Thr Met Asn Arg Glu Asn Val
410 415 420
gat ggt ttt gac ggc agt tca tcc aag gaa cag gtg ctg ttt gat atg 1411
Asp Gly Phe Asp Gly Ser Ser Ser Lys Glu Gln Val Leu Phe Asp Met
425 430 435
ttc agc aac ctt ccg ctg tac tcg atc aca ccg ttc att ttg atc ttt 1459
Phe Ser Asn Leu Pro Leu Tyr Ser Ile Thr Pro Phe Ile Leu Ile Phe
440 445 450
gtg ctg gca gta ttc ttt gtt acc tct gcc gat tcc gcc tcc gtg gtg 1507
Val Leu Ala Val Phe Phe Val Thr Ser Ala Asp Ser Ala Ser Val Val
455 460 465
atg gga acg atg agc tcc caa ggt aac cct gca cca aac aaa tta atc 1555
Met Gly Thr Met Ser Ser Gln Gly Asn Pro Ala Pro Asn Lys Leu Ile
470 475 480 485
gtg gtg ttc tgg gga ctg tgc atg atg ggc atc gcg gtg gtc atg ctg 1603
Val Val Phe Trp Gly Leu Cys Met Met Gly Ile Ala Val Val Met Leu
490 495 500
ctt act ggt ggc gaa tcc gcg ctg act ggt ctg cag aac ctc acc att 1651
Leu Thr Gly Gly Glu Ser Ala Leu Thr Gly Leu Gln Asn Leu Thr Ile
505 510 515
ttg atc gcc att ccg ttt gcg ctg gtg ttg atc gtg atg gct att gcc 1699
Leu Ile Ala Ile Pro Phe Ala Leu Val Leu Ile Val Met Ala Ile Ala
520 525 530
ttt att aag gac tta tcc aca gac cca gcc gct att cga caa cgc tat 1747
Phe Ile Lys Asp Leu Ser Thr Asp Pro Ala Ala Ile Arg Gln Arg Tyr
535 540 545
gca aag gca gcc atc tct aac gcg gtg gtt cgt ggc ttg gaa gaa cac 1795
Ala Lys Ala Ala Ile Ser Asn Ala Val Val Arg Gly Leu Glu Glu His
550 555 560 565
ggc gac gac ttc gag ctc tcc atc gaa cct gca gag gaa ggt cgt gga 1843
Gly Asp Asp Phe Glu Leu Ser Ile Glu Pro Ala Glu Glu Gly Arg Gly
570 575 580
gcg ggt gct acc ttc gat tcc acc gct gat cac atc acc gac tgg tat 1891
Ala Gly Ala Thr Phe Asp Ser Thr Ala Asp His Ile Thr Asp Trp Tyr
585 590 595
cag cgc acc gac gaa gaa ggc aat gat gtt gat tat gac ttc acc acc 1939
Gln Arg Thr Asp Glu Glu Gly Asn Asp Val Asp Tyr Asp Phe Thr Thr
600 605 610
ggc aaa tgg gcc gat ggt tgg aca ccg gaa tcg acc gaa gaa ggc gaa 1987
Gly Lys Trp Ala Asp Gly Trp Thr Pro Glu Ser Thr Glu Glu Gly Glu
615 620 625
gtg gac gcg aaa aag gat taaaaaataa cgactggctg ggattcacac 2035
Val Asp Ala Lys Lys Asp
630 635
<210>298
<211>635
<212>PRT
<213>谷氨酸棒状杆菌
<400>298
Met Glu Arg Thr His Met Ser Thr Asn Ser Gly Asn Asn Leu Pro Glu
1 5 10 15
Ser Gln Glu Ser Pro Glu Glu Pro His Tyr Pro His Asp Thr His Pro
20 25 30
Gly Leu Val Pro Gly Ile Ser Val Asp Ala Gln Arg Asn Lys Phe Gly
35 40 45
Leu Asp Lys Thr Val Phe Gly Val Thr Ala Ala Leu Ile Leu Ala Phe
50 55 60
Ile Ala Trp Gly Ile Ser Ser Pro Asp Ser Val Ser Ser Val Ser Ser
65 70 75 80
Thr Met Phe Ser Trp Ala Met Thr Asn Thr Gly Trp Leu Leu Asn Phe
85 90 95
Val Met Leu Ile Gly Ile Gly Thr Met Leu Tyr Ile Ala Phe Ser Arg
100 105 110
Tyr Gly Arg Ile Lys Leu Gly Thr Asp Glu Asp Glu Pro Glu Phe Ser
115 120 125
Arg Phe Ser Trp Ile Ala Met Met Phe Gly Ala Gly Ile Gly Val Gly
130 135 140
Ile Phe Phe Phe Gly Pro Ser Glu Pro Leu Trp His Tyr Leu Ser Pro
145 150 155 160
Pro Pro His Thr Val Glu Gly Ser Thr Pro Glu Ser Leu His Gln Ala
165 170 175
Leu Ala Gln Ser His Phe His Trp Gly Leu Ser Ala Trp Gly Leu Tyr
180 185 190
Ala Leu Val Gly Gly Ala Leu Ala Tyr Ser Ser Tyr Arg Arg Gly Arg
195 200 205
Val Thr Leu Ile Ser Ser Thr Phe Arg Ser Leu Phe Gly Glu Lys Thr
210 215 220
Glu Gly Ile Ala Gly Arg Leu Ile Asp Met Met Ala Ile Ile Ala Thr
225 230 235 240
Leu Phe Gly Thr Ala Ala Thr Leu Gly Leu Ser Ala Ile Gln Val Gly
245 250 255
Gln Gly Val Gln Ile Ile Ser Gly Ala Ser Glu Ile Thr Asn Asn Ile
260 265 270
Leu Ile Ala Ile Ile Ala Ile Leu Thr Ile Gly Phe Ile Ile Ser Ser
275 280 285
Val Ser Gly Val Ser Lys Gly Ile Arg Tyr Leu Ser Asn Leu Asn Ile
290 295 300
Ser Leu Thr Leu Gly Leu Val Leu Phe Val Phe Ile Thr Gly Pro Thr
305 310 315 320
Leu Phe Leu Leu Asn Leu Ile Pro Ser Ser Val Leu Glu Tyr Gly Ser
325 330 335
Glu Phe Leu Ser Met Ala Gly Lys Ser Leu Ser Trp Gly Glu Glu Thr
340 345 350
Ile Glu Phe Gln Ala Gly Trp Thr Ala Phe Tyr Trp Ala Trp Trp Ile
355 360 365
Ala Trp Thr Pro Phe Val Gly Met Phe Ile Ala Arg Ile Ser Arg Gly
370 375 380
Arg Thr Leu Arg Glu Phe Ala Leu Ile Thr Met Ala Ile Pro Ser Phe
385 390 395 400
Ile Leu Ile Leu Ala Phe Thr Ile Phe Gly Gly Thr Ala Ile Thr Met
405 410 415
Asn Arg Glu Asn Val Asp Gly Phe Asp Gly Ser Ser Ser Lys Glu Gln
420 425 430
Val Leu Phe Asp Met Phe Ser Asn Leu Pro Leu Tyr Ser Ile Thr Pro
435 440 445
Phe Ile Leu Ile Phe Val Leu Ala Val Phe Phe Val Thr Ser Ala Asp
450 455 460
Ser Ala Ser Val Val Met Gly Thr Met Ser Ser Gln Gly Asn Pro Ala
465 470 475 480
Pro Asn Lys Leu Ile Val Val Phe Trp Gly Leu Cys Met Met Gly Ile
485 490 495
Ala Val Val Met Leu Leu Thr Gly Gly Glu Ser Ala Leu Thr Gly Leu
500 505 510
Gln Asn Leu Thr Ile Leu Ile Ala Ile Pro Phe Ala Leu Val Leu Ile
515 520 525
Val Met Ala Ile Ala Phe Ile Lys Asp Leu Ser Thr Asp Pro Ala Ala
530 535 540
Ile Arg Gln Arg Tyr Ala Lys Ala Ala Ile Ser Asn Ala Val Val Arg
545 550 555 560
Gly Leu Glu Glu His Gly Asp Asp Phe Glu Leu Ser Ile Glu Pro Ala
565 570 575
Glu Glu Gly Arg Gly Ala Gly Ala Thr Phe Asp Ser Thr Ala Asp His
580 585 590
Ile Thr Asp Trp Tyr Gln Arg Thr Asp Glu Glu Gly Asn Asp Val Asp
595 600 605
Tyr Asp Phe Thr Thr Gly Lys Trp Ala Asp Gly Trp Thr Pro Glu Ser
610 615 620
Thr Glu Glu Gly Glu Val Asp Ala Lys Lys Asp
625 630 635
<210>299
<211>916
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(886)
<223>RXA02425
<400>299
gtatttatca gcgtctgtgg actgcgtgga gtgtcggaag atagttgact gttcaatgcg 60
ttgaacatta gtattcatcc ctatggatac ctcgactgtg ttg ttc gca ttt gga 115
Leu Phe Ala Phe Gly
1 5
ctc acc cta ttt gcc ggt ctt gcc acc ggc atc ggc ggt ctc atc gcc 163
Leu Thr Leu Phe Ala Gly Leu Ala Thr Gly Ile Gly Gly Leu Ile Ala
10 15 20
gta gcg cgc aaa acc gtc acc gaa gga ttc ctc gca gga tca ctc ggc 211
Val Ala Arg Lys Thr Val Thr Glu Gly Phe Leu Ala Gly Ser Leu Gly
25 30 35
ttc tct gtc ggc gtg atg ctg tac gta tcc ttt gtg gaa att cta ccc 259
Phe Ser Val Gly Val Met Leu Tyr Val Ser Phe Val Glu Ile Leu Pro
40 45 50
ggc gcc ttt gat gaa tta acc agt gtt tgg gga gaa aaa ggc gga agt 307
Gly Ala Phe Asp Glu Leu Thr Ser Val Trp Gly Glu Lys Gly Gly Ser
55 60 65
tgg gcc gct gtt atc ggc ttc ttc ggt ggc atc gcg ctg atc gcc atc 355
Trp Ala Ala Val Ile Gly Phe Phe Gly Gly Ile Ala Leu Ile Ala Ile
70 75 80 85
atc gac cgc tta gtc ccc acg gcg att aac ccc cac gag ccc tcc acc 403
Ile Asp Arg Leu Val Pro Thr Ala Ile Asn Pro His Glu Pro Ser Thr
90 95 100
gtg gga ggc gcc gtt gaa gga ttc gag cgc cgc aac cgc atg atg aaa 451
Val Gly Gly Ala Val Glu Gly Phe Glu Arg Arg Asn Arg Met Met Lys
105 110 115
atg ggt gtg ctc act gcg ctg gcc atc gcg atc cac aac ttt ccc gaa 499
Met Gly Val Leu Thr Ala Leu Ala Ile Ala Ile His Asn Phe Pro Glu
120 125 130
ggt ttc gct aca ttc ttg gcc gga ttg tcc gat cca atg atc gcg atc 547
Gly Phe Ala Thr Phe Leu Ala Gly Leu Ser Asp Pro Met Ile Ala Ile
135 140 145
cct gtg gcc gtg gca att gcc att cac aat att cca gag ggc att gcg 595
Pro Val Ala Val Ala Ile Ala Ile His Asn Ile Pro Glu Gly Ile Ala
150 155 160 165
gtg gcg gtg cca ctg agg gaa gct aca ggg tca cgt cga aaa gca tta 643
Val Ala Val Pro Leu Arg Glu Ala Thr Gly Ser Arg Arg Lys Ala Leu
170 175 180
ggt tgg gcg acc ctc tcc ggc ctt gct gag ccc gcc ggc gcc ctc atc 691
Gly Trp Ala Thr Leu Ser Gly Leu Ala Glu Pro Ala Gly Ala Leu Ile
185 190 195
gga ttc ctg ctg ctc atg ccc ttt atc gga cca gaa gca ctc ggc ctg 739
Gly Phe Leu Leu Leu Met Pro Phe Ile Gly Pro Glu Ala Leu Gly Leu
200 205 210
tgc ttc gcc gca gtt gcc ggc gtg atg gtg ttc atc agc gtc gat gaa 787
Cys Phe Ala Ala Val Ala Gly Val Met Val Phe Ile Ser Val Asp Glu
215 220 225
cta ctg ccc act gcc atc tcc agc ggc aaa cac cac acc gcc atc tac 835
Leu Leu Pro Thr Ala Ile Ser Ser Gly Lys His His Thr Ala Ile Tyr
230 235 240 245
gga ctc atc gct ggc atg gca gtc atg gcg atc agc ctg ctg ctg ttt 883
Gly Leu Ile Ala Gly Met Ala Val Met Ala Ile Ser Leu Leu Leu Phe
250 255 260
atc tagcgttcag ccgcaacaag catccggtca 916
Ile
<210>300
<211>262
<212>PRT
<213>谷氨酸棒状杆菌
<400>300
Leu Phe Ala Phe Gly Leu Thr Leu Phe Ala Gly Leu Ala Thr Gly Ile
1 5 10 15
Gly Gly Leu Ile Ala Val Ala Arg Lys Thr Val Thr Glu Gly Phe Leu
20 25 30
Ala Gly Ser Leu Gly Phe Ser Val Gly Val Met Leu Tyr Val Ser Phe
35 40 45
Val Glu Ile Leu Pro Gly Ala Phe Asp Glu Leu Thr Ser Val Trp Gly
50 55 60
Glu Lys Gly Gly Ser Trp Ala Ala Val Ile Gly Phe Phe Gly Gly Ile
65 70 75 80
Ala Leu Ile Ala Ile Ile Asp Arg Leu Val Pro Thr Ala Ile Asn Pro
85 90 95
His Glu Pro Ser Thr Val Gly Gly Ala Val Glu Gly Phe Glu Arg Arg
100 105 110
Asn Arg Met Met Lys Met Gly Val Leu Thr Ala Leu Ala Ile Ala Ile
115 120 125
His Asn Phe Pro Glu Gly Phe Ala Thr Phe Leu Ala Gly Leu Ser Asp
130 135 140
Pro Met Ile Ala Ile Pro Val Ala Val Ala Ile Ala Ile His Asn Ile
145 150 155 160
Pro Glu Gly Ile Ala Val Ala Val Pro Leu Arg Glu Ala Thr Gly Ser
165 170 175
Arg Arg Lys Ala Leu Gly Trp Ala Thr Leu Ser Gly Leu Ala Glu Pro
180 185 190
Ala Gly Ala Leu Ile Gly Phe Leu Leu Leu Met Pro Phe Ile Gly Pro
195 200 205
Glu Ala Leu Gly Leu Cys Phe Ala Ala Val Ala Gly Val Met Val Phe
210 215 220
Ile Ser Val Asp Glu Leu Leu Pro Thr Ala Ile Ser Ser Gly Lys His
225 230 235 240
His Thr Ala Ile Tyr Gly Leu Ile Ala Gly Met Ala Val Met Ala Ile
245 250 255
Ser Leu Leu Leu Phe Ile
260
<210>301
<211>682
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(652)
<223>RXA02433
<400>301
atgctgatag atatttggtg accagagatt agagcaaagg atcattttca tcgtgaagct 60
tttcaaggca accgcagtta cttttactgt tgcagcagca ttg gcg ctc agc gcg 115
Leu Ala Leu Ser Ala
1 5
tgc tcc agc agt gat gat tcc tct tcg gag tca agc acc tct tct tcc 163
Cys Ser Ser Ser Asp Asp Ser Ser Ser Glu Ser Ser Thr Ser Ser Ser
10 15 20
acc tct tcg gct gcg tct gat gct gcg act cag tac cca act gct gag 211
Thr Ser Ser Ala Ala Ser Asp Ala Ala Thr Gln Tyr Pro Thr Ala Glu
25 30 35
gaa ctg aat gca att ttg gct gtg gca acc gac cct gag gca cca atc 259
Glu Leu Asn Ala Ile Leu Ala Val Ala Thr Asp Pro Glu Ala Pro Ile
40 45 50
gag gag aag gtg aag act gtt cag ggt tct gag aac gct cct gag ctg 307
Glu Glu Lys Val Lys Thr Val Gln Gly Ser Glu Asn Ala Pro Glu Leu
55 60 65
ttt gag acc atg act cag gca aag gtg gag tcg ggt gct gag ttc cag 355
Phe Glu Thr Met Thr Gln Ala Lys Val Glu Ser Gly Ala Glu Phe Gln
70 75 80 85
gtt gtt ggt tct gtg ctt cct ggc tat gac cca acc tct gag gtt ctg 403
Val Val Gly Ser Val Leu Pro Gly Tyr Asp Pro Thr Ser Glu Val Leu
90 95 100
acc acc gtc atg ttc cag ctt ccg gac cgc gct gag cag gaa gct gaa 451
Thr Thr Val Met Phe Gln Leu Pro Asp Arg Ala Glu Gln Glu Ala Glu
105 110 115
ggc gtg gag ttt gtg aac act gat ggt aat tgg cag ctg tcc cag gat 499
Gly Val Glu Phe Val Asn Thr Asp Gly Asn Trp Gln Leu Ser Gln Asp
120 125 130
tgg gct tgt atc ttg atc acc aat act gtt gct cct gag cag gtt cct 547
Trp Ala Cys Ile Leu Ile Thr Asn Thr Val Ala Pro Glu Gln Val Pro
135 140 145
gca atg tgt gtt gga act gat gct tct tcc gct ggt att gaa gag gcc 595
Ala Met Cys Val Gly Thr Asp Ala Ser Ser Ala Gly Ile Glu Glu Ala
150 155 160 165
cca gct gaa gag ggt gct gtt gtt gag gaa gcc cct gtt gaa gag gtt 643
Pro Ala Glu Glu Gly Ala Val Val Glu Glu Ala Pro Val Glu Glu Val
170 175 180
cct gtt cag tagggtttaa cggctcaggc tgattttctt 682
Pro Val Gln
<210>302
<211>184
<212>PRT
<213>谷氨酸棒状杆菌
<400>302
Leu Ala Leu Ser Ala Cys Ser Ser Ser Asp Asp Ser Ser Ser Glu Ser
1 5 10 15
Ser Thr Ser Ser Ser Thr Ser Ser Ala Ala Ser Asp Ala Ala Thr Gln
20 25 30
Tyr Pro Thr Ala Glu Glu Leu Asn Ala Ile Leu Ala Val Ala Thr Asp
35 40 45
Pro Glu Ala Pro Ile Glu Glu Lys Val Lys Thr Val Gln Gly Ser Glu
50 55 60
Asn Ala Pro Glu Leu Phe Glu Thr Met Thr Gln Ala Lys Val Glu Ser
65 70 75 80
Gly Ala Glu Phe Gln Val Val Gly Ser Val Leu Pro Gly Tyr Asp Pro
85 90 95
Thr Ser Glu Val Leu Thr Thr Val Met Phe Gln Leu Pro Asp Arg Ala
100 105 110
Glu Gln Glu Ala Glu Gly Val Glu Phe Val Asn Thr Asp Gly Asn Trp
115 120 125
Gln Leu Ser Gln Asp Trp Ala Cys Ile Leu Ile Thr Asn Thr Val Ala
130 135 140
Pro Glu Gln Val Pro Ala Met Cys Val Gly Thr Asp Ala Ser Ser Ala
145 150 155 160
Gly Ile Glu Glu Ala Pro Ala Glu Glu Gly Ala Val Val Glu Glu Ala
165 170 175
Pro Val Glu Glu Val Pro Val Gln
180
<210>303
<211>766
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(736)
<223>RXA02486
<400>303
tccgacctcg tcgcattagc caaagaaatc cgcgacggag tcctcgaaac cttcggcgtc 60
accctcgtcc cagaacccgt ctggattgga atcagcatcg atg act gaa ttt tcc 115
Met Thr Glu Phe Ser
1 5
gac gtc cct ggc acc gcc gcc cca ctg cac agg gcg ttg gaa aat gcc 163
Asp Val Pro Gly Thr Ala Ala Pro Leu His Arg Ala Leu Glu Asn Ala
10 15 20
gga tac tcc act tta gaa tcc ctc gac ggt gtt ccc tac aag acg ttg 211
Gly Tyr Ser Thr Leu Glu Ser Leu Asp Gly Val Pro Tyr Lys Thr Leu
25 30 35
att gcc ctg cat ggt gtc gga aaa act ggc ctc ggc agg atc cag gca 259
Ile Ala Leu His Gly Val Gly Lys Thr Gly Leu Gly Arg Ile Gln Ala
40 45 50
gca ttg ttg gaa cgt ggc ctt tcc ctg ggg gaa gaa aca aaa ggc gcc 307
Ala Leu Leu Glu Arg Gly Leu Ser Leu Gly Glu Glu Thr Lys Gly Ala
55 60 65
acc atc acc cca ggt cac acc ggt aaa gtg gcc tca gat atc aaa act 355
Thr Ile Thr Pro Gly His Thr Gly Lys Val Ala Ser Asp Ile Lys Thr
70 75 80 85
cac atc act tcc gtg gat ccc gtc gca tac gtc gat ggt cta gag ggg 403
His Ile Thr Ser Val Asp Pro Val Ala Tyr Val Asp Gly Leu Glu Gly
90 95 100
cgt cgg gtt gct cac ggg cac caa ttg cta tcg atc ttc ggt cgc gtc 451
Arg Arg Val Ala His Gly His Gln Leu Leu Ser Ile Phe Gly Arg Val
105 110 115
acc ggc gcg gaa ccc aaa atg tgg gga ccc tcc atg atc gga tac ggc 499
Thr Gly Ala Glu Pro Lys Met Trp Gly Pro Ser Met Ile Gly Tyr Gly
120 125 130
tca gtc cac tac gtt tcc cac acc gga agg gaa ggc gat tgg ttt cag 547
Ser Val His Tyr Val Ser His Thr Gly Arg Glu Gly Asp Trp Phe Gln
135 140 145
tgt ggt ttc agc ccg gcg aag tcc aaa atc tgc ctg tat ggc ctg aag 595
Cys Gly Phe Ser Pro Ala Lys Ser Lys Ile Cys Leu Tyr Gly Leu Lys
150 155 160 165
gat tcg cct cgc ggt gag gaa ttg ctg cag aaa ctt gga aaa tac acc 643
Asp Ser Pro Arg Gly Glu Glu Leu Leu Gln Lys Leu Gly Lys Tyr Thr
170 175 180
gaa ggc cgc gga tgc gtg tac atc aat aaa ccg gaa gac atc gat ttg 691
Glu Gly Arg Gly Cys Val Tyr Ile Asn Lys Pro Glu Asp Ile Asp Leu
185 190 195
gat gtt tta gag gcc atg atc agc gag tca tgg gcc ggc caa ggc 736
Asp Val Leu Glu Ala Met Ile Ser Glu Ser Trp Ala Gly Gln Gly
200 205 210
taggttgcaa atccccacca caagttgctc 766
<210>304
<211>212
<212>PRT
<213>谷氨酸棒状杆菌
<400>304
Met Thr Glu Phe Ser Asp Val Pro Gly Thr Ala Ala Pro Leu His Arg
1 5 10 15
Ala Leu Glu Asn Ala Gly Tyr Ser Thr Leu Glu Ser Leu Asp Gly Val
20 25 30
Pro Tyr Lys Thr Leu Ile Ala Leu His Gly Val Gly Lys Thr Gly Leu
35 40 45
Gly Arg Ile Gln Ala Ala Leu Leu Glu Arg Gly Leu Ser Leu Gly Glu
50 55 60
Glu Thr Lys Gly Ala Thr Ile Thr Pro Gly His Thr Gly Lys Val Ala
65 70 75 80
Ser Asp Ile Lys Thr His Ile Thr Ser Val Asp Pro Val Ala Tyr Val
85 90 95
Asp Gly Leu Glu Gly Arg Arg Val Ala His Gly His Gln Leu Leu Ser
100 105 110
Ile Phe Gly Arg Val Thr Gly Ala Glu Pro Lys Met Trp Gly Pro Ser
115 120 125
Met Ile Gly Tyr Gly Ser Val His Tyr Val Ser His Thr Gly Arg Glu
130 135 140
Gly Asp Trp Phe Gln Cys Gly Phe Ser Pro Ala Lys Ser Lys Ile Cys
145 150 155 160
Leu Tyr Gly Leu Lys Asp Ser Pro Arg Gly Glu Glu Leu Leu Gln Lys
165 170 175
Leu Gly Lys Tyr Thr Glu Gly Arg Gly Cys Val Tyr Ile Asn Lys Pro
180 185 190
Glu Asp Ile Asp Leu Asp Val Leu Glu Ala Met Ile Ser Glu Ser Trp
195 200 205
Ala Gly Gln Gly
210
<210>305
<211>1087
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1057)
<223>RXA024 88
<400>305
taaggttacc ctaccctctt tggggtaggg ctcttccatt tttgccaggt taggttaagc 60
taatctagtt tttattgcgt tatcgaacag gagaacaaaa atg aac aag ctt gcc 115
Met Asn Lys Leu Ala
1 5
acc cgt gcg ctc gtt gct ctc acg gga tcc gcc atc gcc atg acc ggc 163
Thr Arg Ala Leu Val Ala Leu Thr Gly Ser Ala Ile Ala Met Thr Gly
10 15 20
ctc acc gta gtc tcg gct aat gca gcc gag aaa acc ggc aaa tgc cgc 211
Leu Thr Val Val Ser Ala Asn Ala Ala Glu Lys Thr Gly Lys Cys Arg
25 30 35
gtt gtc acc acc aca gga acc gcc gac tgg tca gtg cgt gaa tca ttc 259
Val Val Thr Thr Thr Gly Thr Ala Asp Trp Ser Val Arg Glu Ser Phe
40 45 50
aac aac tac ctt gaa ggt cca att gca aac ggt gca gca tac aaa tac 307
Asn Asn Tyr Leu Glu Gly Pro Ile Ala Asn Gly Ala Ala Tyr Lys Tyr
55 60 65
cac ggt ggc atc gag gtc cgt gac ggt gta gaa acc acc ggc acg aag 355
His Gly Gly Ile Glu Val Arg Asp Gly Val Glu Thr Thr Gly Thr Lys
70 75 80 85
tca gct cgc gag ttc acc tgg cca gta ctt ggc agc gaa gaa gga gcc 403
Ser Ala Arg Glu Phe Thr Trp Pro Val Leu Gly Ser Glu Glu Gly Ala
90 95 100
gtc aaa ctt ggc ggt ggc gtt cac tgg acc gga cac aat cac tac tcc 451
Val Lys Leu Gly Gly Gly Val His Trp Thr Gly His Asn His Tyr Ser
105 110 115
ggc gac gac gag tcc cag gcg cca gac aac ttc atc ctt gat ctc gac 499
Gly Asp Asp Glu Ser Gln Ala Pro Asp Asn Phe Ile Leu Asp Leu Asp
120 125 130
ttc tcc aac cca acc gtc aaa ttc gat ggc aac gag ggc acc ctg ctt 547
Phe Ser Asn Pro Thr Val Lys Phe Asp Gly Asn Glu Gly Thr Leu Leu
135 140 145
gtc gac ttc aag tcc cgt gag ttc gtt gat acc aag acc gtc gct gac 595
Val Asp Phe Lys Ser Arg Glu Phe Val Asp Thr Lys Thr Val Ala Asp
150 155 160 165
ttc ctc acc gga act cag gca gag ttg gct acc atc act ttc gat gag 643
Phe Leu Thr Gly Thr Gln Ala Glu Leu Ala Thr Ile Thr Phe Asp Glu
170 175 180
cca atc gac ctg acc cag gag aac gtc acc gtc acc ggc cag acc aag 691
Pro Ile Asp Leu Thr Gln Glu Asn Val Thr Val Thr Gly Gln Thr Lys
185 190 195
ctg act gca act ggt gtc gat gtc atg ggc acc ttc tac cca gaa ggt 739
Leu Thr Ala Thr Gly Val Asp Val Met Gly Thr Phe Tyr Pro Glu Gly
200 205 210
gaa gca ctt gcg cca atc act ttg aac ctc acc aac gaa gtt gta tgc 787
Glu Ala Leu Ala Pro Ile Thr Leu Asn Leu Thr Asn Glu Val Val Cys
215 220 225
gac gag cct gaa acc ccc gtt gag cct gag gtt cct gta gag cct gag 835
Asp Glu Pro Glu Thr Pro Val Glu Pro Glu Val Pro Val Glu Pro Glu
230 235 240 245
act cca gta gat ccg gaa acc tcg gtc gac cca gag act cca gta gat 883
Thr Pro Val Asp Pro Glu Thr Ser Val Asp Pro Glu Thr Pro Val Asp
250 255 260
ccg gaa acc tcg gtc gac cca gaa aag cct ggc gat gac aac aag gat 931
Pro Glu Thr Ser Val Asp Pro Glu Lys Pro Gly Asp Asp Asn Lys Asp
265 270 275
gac gga agc aac agc agc agc aac ggc gat atc ctc gga atc ttg ggc 979
Asp Gly Ser Asn Ser Ser Ser Asn Gly Asp Ile Leu Gly Ile Leu Gly
280 285 290
atc ctc gca gcg ctt ggt ggc gtt ggt gca ctt gtg tac aac ttc ctg 1027
Ile Leu Ala Ala Leu Gly Gly Val Gly Ala Leu Val Tyr Asn Phe Leu
295 300 305
gta gct agc gga ttc ctt gca gcg ttt aag taaatgaatc gttttcctag 1077
Val Ala Ser Gly Phe Leu Ala Ala Phe Lys
310 315
tgccttatta 1087
<210>306
<211>319
<212>PRT
<213>谷氨酸棒状杆菌
<400>306
Met Asn Lys Leu Ala Thr Arg Ala Leu Val Ala Leu Thr Gly Ser Ala
1 5 10 15
Ile Ala Met Thr Gly Leu Thr Val Val Ser Ala Asn Ala Ala Glu Lys
20 25 30
Thr Gly Lys Cys Arg Val Val Thr Thr Thr Gly Thr Ala Asp Trp Ser
35 40 45
Val Arg Glu Ser Phe Asn Asn Tyr Leu Glu Gly Pro Ile Ala Asn Gly
50 55 60
Ala Ala Tyr Lys Tyr His Gly Gly Ile Glu Val Arg Asp Gly Val Glu
65 70 75 80
Thr Thr Gly Thr Lys Ser Ala Arg Glu Phe Thr Trp Pro Val Leu Gly
85 90 95
Ser Glu Glu Gly Ala Val Lys Leu Gly Gly Gly Val His Trp Thr Gly
100 105 110
His Asn His Tyr Ser Gly Asp Asp Glu Ser Gln Ala Pro Asp Asn Phe
115 120 125
Ile Leu Asp Leu Asp Phe Ser Asn Pro Thr Val Lys Phe Asp Gly Asn
130 135 140
Glu Gly Thr Leu Leu Val Asp Phe Lys Ser Arg Glu Phe Val Asp Thr
145 150 155 160
Lys Thr Val Ala Asp Phe Leu Thr Gly Thr Gln Ala Glu Leu Ala Thr
165 170 175
Ile Thr Phe Asp Glu Pro Ile Asp Leu Thr Gln Glu Asn Val Thr Val
180 185 190
Thr Gly Gln Thr Lys Leu Thr Ala Thr Gly Val Asp Val Met Gly Thr
195 200 205
Phe Tyr Pro Glu Gly Glu Ala Leu Ala Pro Ile Thr Leu Asn Leu Thr
210 215 220
Asn Glu Val Val Cys Asp Glu Pro Glu Thr Pro Val Glu Pro Glu Val
225 230 235 240
Pro Val Glu Pro Glu Thr Pro Val Asp Pro Glu Thr Ser Val Asp Pro
245 250 255
Glu Thr Pro Val Asp Pro Glu Thr Ser Val Asp Pro Glu Lys Pro Gly
260 265 270
Asp Asp Asn Lys Asp Asp Gly Ser Asn Ser Ser Ser Asn Gly Asp Ile
275 280 285
Leu Gly Ile Leu Gly Ile Leu Ala Ala Leu Gly Gly Val Gly Ala Leu
290 295 300
Val Tyr Asn Phe Leu Val Ala Ser Gly Phe Leu Ala Ala Phe Lys
305 310 315
<210>307
<211>2716
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2686)
<223>RXA02495
<400>307
aactttaagg agttcaccaa ggaggaaaat tatcaccgtc ttaaaaacgg ctaaggcttt 60
tctaaattcg ccgctgcgca ccatcacgtg gcgcagcgtt gtg gcg aat aaa ctc 115
Val Ala Asn Lys Leu
1 5
cgc atg gca tta aca gtg ctt tcg gtt gtg cta gga acc gca ttt ttg 163
Arg Met Ala Leu Thr Val Leu Ser Val Val Leu Gly Thr Ala Phe Leu
10 15 20
tgt ggc tcc ctc ctc ctc aca cat tcc ctt gaa aga aca ttt tcc tcc 211
Cys Gly Ser Leu Leu Leu Thr His Ser Leu Glu Arg Thr Phe Ser Ser
25 30 35
atc gtt gat gcc ggc gtg gag ggc gtt gac gtg ggc gtt atc gcg caa 259
Ile Val Asp Ala Gly Val Glu Gly Val Asp Val Gly Val Ile Ala Gln
40 45 50
caa aac aac ccc gac ggc gta cct ttc tcc gtc atc gcc gaa att gag 307
Gln Asn Asn Pro Asp Gly Val Pro Phe Ser Val Ile Ala Glu Ile Glu
55 60 65
cag tat ccg gaa gtc cgc gcc gtc aac atc atc ggc gac ggc ccc ggc 355
Gln Tyr Pro Glu Val Arg Ala Val Asn Ile Ile Gly Asp Gly Pro Gly
70 75 80 85
atg ccc tcc ggc acc acc atg acc ggc caa tcc gcg ctg atc ctc acc 403
Met Pro Ser Gly Thr Thr Met Thr Gly Gln Ser Ala Leu Ile Leu Thr
90 95 100
gac tcc gac ggc aat ccc ctg caa gca ggc agc tcc ggc acg cac ccg 451
Asp Ser Asp Gly Asn Pro Leu Gln Ala Gly Ser Ser Gly Thr His Pro
105 110 115
cta gcc att tat ccg cag ggc gag tgg gtc tcg ccc gag cca acg ctt 499
Leu Ala Ile Tyr Pro Gln Gly Glu Trp Val Ser Pro Glu Pro Thr Leu
120 125 130
atc gac ggc cac ttc ccc aca aaa ccc gac gag gta gta gtc aac gct 547
Ile Asp Gly His Phe Pro Thr Lys Pro Asp Glu Val Val Val Asn Ala
135 140 145
tcg gcc gcc aaa cgt ggc ggg ctc tcc ctc ggt gat cac cta acg atc 595
Ser Ala Ala Lys Arg Gly Gly Leu Ser Leu Gly Asp His Leu Thr Ile
150 155 160 165
gtc aca ccc acc gaa cgc atc gac gcc acc ctg tct gga acc ttc gaa 643
Val Thr Pro Thr Glu Arg Ile Asp Ala Thr Leu Ser Gly Thr Phe Glu
170 175 180
tca aac acc gac gtc gca ggc tgg gtc gga gtc gga ttc acc cca cag 691
Ser Asn Thr Asp Val Ala Gly Trp Val Gly Val Gly Phe Thr Pro Gln
185 190 195
cgc tac gtc gaa ctg ttc acc aac ggc acc gac gcc agc caa atc acc 739
Arg Tyr Val Glu Leu Phe Thr Asn Gly Thr Asp Ala Ser Gln Ile Thr
200 205 210
atc gca gtc aac gac ggc gcc gac ccc atg gca gtc cgc aac cgc atc 787
Ile Ala Val Asn Asp Gly Ala Asp Pro Met Ala Val Arg Asn Arg Ile
215 220 225
ggc aaa aac cac cgc gac ctc ctg ccc ctg ctg ccc gag caa atc atc 835
Gly Lys Asn His Arg Asp Leu Leu Pro Leu Leu Pro Glu Gln Ile Ile
230 235 240 245
gac caa acc acc ggc gac acc gcc cgc caa ctg gag ttc atg acc tac 883
Asp Gln Thr Thr Gly Asp Thr Ala Arg Gln Leu Glu Phe Met Thr Tyr
250 255 260
gtg ctc ctg gca ttc gcc gcg atc gca ctg att gtt ggc tcg ttc atc 931
Val Leu Leu Ala Phe Ala Ala Ile Ala Leu Ile Val Gly Ser Phe Ile
265 270 275
att gcc aac acc ttc gcg atg atc gtc gcc caa cgc acc ggc gaa ttc 979
Ile Ala Asn Thr Phe Ala Met Ile Val Ala Gln Arg Thr Gly Glu Phe
280 285 290
gcc ctc ctc cgt tcc att ggt gtc tcc acg ttc caa atc gga ttc tcc 1027
Ala Leu Leu Arg Ser Ile Gly Val Ser Thr Phe Gln Ile Gly Phe Ser
295 300 305
gtg att atg gaa gca gta ttc gtc gga ctt atc ggc ggt ttc atc ggc 1075
Val Ile Met Glu Ala Val Phe Val Gly Leu Ile Gly Gly Phe Ile Gly
310 315 320 325
atc gca gtt ggc ttc gga gtg gtc aat gca ctt gtc caa gtg ctc aac 1123
Ile Ala Val Gly Phe Gly Val Val Asn Ala Leu Val Gln Val Leu Asn
330 335 340
caa ttc ggc gac acc ctc tcc tcc atc gac atc acc tac aac gcc gga 1171
Gln Phe Gly Asp Thr Leu Ser Ser Ile Asp Ile Thr Tyr Asn Ala Gly
345 350 355
tcc ttc atc ttc cct gtc ctc ttc gcc gtc act gcc acc gta cta agc 1219
Ser Phe Ile Phe Pro Val Leu Phe Ala Val Thr Ala Thr Val Leu Ser
360 365 370
tcc ata tcg cct gct cac cgc gcc ggt aac ctc cca cca gtc caa gca 1267
Ser Ile Ser Pro Ala His Arg Ala Gly Asn Leu Pro Pro Val Gln Ala
375 380 385
ttc gaa tcc tcc gat gca cgc agc gac gcc ctc gga agg atc cgc ttc 1315
Phe Glu Ser Ser Asp Ala Arg Ser Asp Ala Leu Gly Arg Ile Arg Phe
390 395 400 405
ctc gtc gcc gcc gtc atg ctc acc ctt gga atc agc ctg acg atc gca 1363
Leu Val Ala Ala Val Met Leu Thr Leu Gly Ile Ser Leu Thr Ile Ala
410 415 420
ggt gcc gtg gta tcc gcc atc aac gga gat gaa ttc aaa aca gaa aca 1411
Gly Ala Val Val Ser Ala Ile Asn Gly Asp Glu Phe Lys Thr Glu Thr
425 430 435
cgc ctc gca ttc atc ggc gct gga ctt ctc tta gtg ttc ttc tca ctc 1459
Arg Leu Ala Phe Ile Gly Ala Gly Leu Leu Leu Val Phe Phe Ser Leu
440 445 450
tcc ctg tcc ggt ccc gca ctg atg gtg gcg acc tct cag act ctt ggc 1507
Ser Leu Ser Gly Pro Ala Leu Met Val Ala Thr Ser Gln Thr Leu Gly
455 460 465
gtg gcg ata atg tcg ccg ttc cga gcc gtc ggc aag ctc gca caa cgc 1555
yal Ala Ile Met Ser Pro Phe Arg Ala Val Gly Lys Leu Ala Gln Arg
470 475 480 485
aac acc ctg cgc aat ccg cga cgc tcc gca acc acc gca ctg gcc gtc 1603
Asn Thr Leu Arg Asn Pro Arg Arg Ser Ala Thr Thr Ala Leu Ala Val
490 495 500
act ttg agc gtt ggc ctc gtc gcc tgc gtt gga gtc atc ggc gcc acc 1651
Thr Leu Ser Val Gly Leu Val Ala Cys Val Gly Val Ile Gly Ala Thr
505 510 515
acc cgc gcc agc gtc ttc ggg tcc atg gaa tcc acc atc aaa tcc cca 1699
Thr Arg Ala Ser Val Phe Gly Ser Met Glu Ser Thr Ile Lys Ser Pro
520 525 530
tat gtc ctc gac agc atc ggt ggc acc atg atc cct gga caa ccc gcc 1747
Tyr Val Leu Asp Ser Ile Gly Gly Thr Met Ile Pro Gly Gln Pro Ala
535 540 545
ggc ggt tca agg tca ctg tcc atg tcc gca gcc gtc gca cag gaa atc 1795
Gly Gly Ser Arg Ser Leu Ser Met Ser Ala Ala Val Ala Gln Glu Ile
550 555 560 565
gca caa acc cgc gga gtc ggc aaa gtg ggc acc ctc atg acc ggc agc 1843
Ala Gln Thr Arg Gly Val Gly Lys Val Gly Thr Leu Met Thr Gly Ser
570 575 580
gtc caa gtc aac gga tgg gac aac gaa aac acc aca att ttc gac ggc 1891
Val Gln Val Asn Gly Trp Asp Asn Glu Asn Thr Thr Ile Phe Asp Gly
585 590 595
gac ctc tct cag ttc ctc gac ctc gca gtc cgc tcc ggc gac gcc ttc 1939
Asp Leu Ser Gln Phe Leu Asp Leu Ala Val Arg Ser Gly Asp Ala Phe
600 605 610
gac gac gaa acc ccc ggc gtc atg atc tcc acc acc tac gcc gac caa 1987
Asp Asp Glu Thr Pro Gly Val Met Ile Ser Thr Thr Tyr Ala Asp Gln
615 620 625
tcc gac ctc gaa gta ggc gac acc gtc acc gtc aac ccc tac gga tcc 2035
Ser Asp Leu Glu Val Gly Asp Thr Val Thr Val Asn Pro Tyr Gly Ser
630 635 640 645
gac gac ggc atc cgc gtc ccc atc acc ggc atc tac gca gaa acc aac 2083
Asp Asp Gly Ile Arg Val Pro Ile Thr Gly Ile Tyr Ala Glu Thr Asn
650 655 660
ctc gtc gga cac ctc atg gtc aac gca gcc gcc acc aac cga gtc ctc 2131
Leu Val Gly His Leu Met Val Asn Ala Ala Ala Thr Asn Arg Val Leu
665 670 675
acc tcc gcg gac acc tac cac cgg tcc caa atc ttc gtc aac ggc gac 2179
Thr Ser Ala Asp Thr Tyr His Arg Ser Gln Ile Phe Val Asn Gly Asp
680 685 690
ggc tcc acc acc aac gaa gaa ctc cgc gac atc ctc gtc gac gcc gta 2227
Gly Ser Thr Thr Asn Glu Glu Leu Arg Asp Ile Leu Val Asp Ala Val
695 700 705
gcc ccc ttc ctc atc gtc caa gtg aaa tcc aaa gat gaa ttc cgc gga 2275
Ala Pro Phe Leu Ile Val Gln Val Lys Ser Lys Asp Glu Phe Arg Gly
710 715 720 725
agc ctc ggc acc caa atc aac caa ttg ctc ggc atc atc tac gga ctg 2323
Ser Leu Gly Thr Gln Ile Asn Gln Leu Leu Gly Ile Ile Tyr Gly Leu
730 735 740
ctc gcc ctg gca gtg atc atc gca gtc ctg gga atc gtc aac aca ctg 2371
Leu Ala Leu Ala Val Ile Ile Ala Val Leu Gly Ile Val Asn Thr Leu
745 750 755
ttc ctc tcc atc agc gaa cgc acc cgc gaa atc gga atc ctc cgc gcc 2419
Phe Leu Ser Ile Ser Glu Arg Thr Arg Glu Ile Gly Ile Leu Arg Ala
760 765 770
acc ggc gtc caa cga gga caa atc cgc cgc atg att acc ctc gaa tcc 2467
Thr Gly Val Gln Arg Gly Gln Ile Arg Arg Met Ile Thr Leu Glu Ser
775 780 785
gtc atc ctc tcc atc cac ggc gca atc cac gga ctc ctc cta ggt aca 2515
Val Ile Leu Ser Ile His Gly Ala Ile His Gly Leu Leu Leu Gly Thr
790 795 800 805
ttt atc ggc tgg gca atc gtc agt tgt ctc cgc acc cga ggc atg gca 2563
Phe Ile Gly Trp Ala Ile Val Ser Cys Leu Arg Thr Arg Gly Met Ala
810 815 820
ccc gtc gaa ttc ccc tgg acc caa atc gga tta atg ctc atc tcc gca 2611
Pro Val Glu Phe Pro Trp Thr Gln Ile Gly Leu Met Leu Ile Ser Ala
825 830 835
atc atc atc gga ggc ata gca gcc ctc atc cca gcc aac cga gcc tcc 2659
Ile Ile Ile Gly Gly Ile Ala Ala Leu Ile Pro Ala Asn Arg Ala Ser
840 845 850
cga atc tcc cct ttg gaa gca atc aac taacagtctg catcggcaac 2706
Arg Ile Ser Pro Leu Glu Ala Ile Asn
855 860
ctcctcttac 2716
<210>308
<211>862
<212>PRT
<213>谷氨酸棒状杆菌
<400>308
Val Ala Asn Lys Leu Arg Met Ala Leu Thr Val Leu Ser Val Val Leu
1 5 10 15
Gly Thr Ala Phe Leu Cys Gly Ser Leu Leu Leu Thr His Ser Leu Glu
20 25 30
Arg Thr Phe Ser Ser Ile Val Asp Ala Gly Val Glu Gly Val Asp Val
35 40 45
Gly Val Ile Ala Gln Gln Asn Asn Pro Asp Gly Val Pro Phe Ser Val
50 55 60
Ile Ala Glu Ile Glu Gln Tyr Pro Glu Val Arg Ala Val Asn Ile Ile
65 70 75 80
Gly Asp Gly Pro Gly Met Pro Ser Gly Thr Thr Met Thr Gly Gln Ser
85 90 95
Ala Leu Ile Leu Thr Asp Ser Asp Gly Asn Pro Leu Gln Ala Gly Ser
100 105 110
Ser Gly Thr His Pro Leu Ala Ile Tyr Pro Gln Gly Glu Trp Val Ser
115 120 125
Pro Glu Pro Thr Leu Ile Asp Gly His Phe Pro Thr Lys Pro Asp Glu
130 135 140
Val Val Val Asn Ala Ser Ala Ala Lys Arg Gly Gly Leu Ser Leu Gly
145 150 155 160
Asp His Leu Thr Ile Val Thr Pro Thr Glu Arg Ile Asp Ala Thr Leu
165 170 175
Ser Gly Thr Phe Glu Ser Asn Thr Asp Val Ala Gly Trp Val Gly Val
180 185 190
Gly Phe Thr Pro Gln Arg Tyr Val Glu Leu Phe Thr Asn Gly Thr Asp
195 200 205
Ala Ser Gln Ile Thr Ile Ala Val Asn Asp Gly Ala Asp Pro Met Ala
210 215 220
Val Arg Asn Arg Ile Gly Lys Asn His Arg Asp Leu Leu Pro Leu Leu
225 230 235 240
Pro Glu Gln Ile Ile Asp Gln Thr Thr Gly Asp Thr Ala Arg Gln Leu
245 250 255
Glu Phe Met Thr Tyr Val Leu Leu Ala Phe Ala Ala Ile Ala Leu Ile
260 265 270
Val Gly Ser Phe Ile Ile Ala Asn Thr Phe Ala Met Ile Val Ala Gln
275 280 285
Arg Thr Gly Glu Phe Ala Leu Leu Arg Ser Ile Gly Val Ser Thr Phe
290 295 300
Gln Ile Gly Phe Ser Val Ile Met Glu Ala Val Phe Val Gly Leu Ile
305 310 315 320
Gly Gly Phe Ile Gly Ile Ala Val Gly Phe Gly Val Val Asn Ala Leu
325 330 335
Val Gln Val Leu Asn Gln Phe Gly Asp Thr Leu Ser Ser Ile Asp Ile
340 345 350
Thr Tyr Asn Ala Gly Ser Phe Ile Phe Pro Val Leu Phe Ala Val Thr
355 360 365
Ala Thr Val Leu Ser Ser Ile Ser Pro Ala His Arg Ala Gly Asn Leu
370 375 380
Pro Pro Val Gln Ala Phe Glu Ser Ser Asp Ala Arg Ser Asp Ala Leu
385 390 395 400
Gly Arg Ile Arg Phe Leu Val Ala Ala Val Met Leu Thr Leu Gly Ile
405 410 415
Ser Leu Thr Ile Ala Gly Ala Val Val Ser Ala Ile Asn Gly Asp Glu
420 425 430
Phe Lys Thr Glu Thr Arg Leu Ala Phe Ile Gly Ala Gly Leu Leu Leu
435 440 445
Val Phe Phe Ser Leu Ser Leu Ser Gly Pro Ala Leu Met Val Ala Thr
450 455 460
Ser Gln Thr Leu Gly Val Ala Ile Met Ser Pro Phe Arg Ala Val Gly
465 470 475 480
Lys Leu Ala Gln Arg Asn Thr Leu Arg Asn Pro Arg Arg Ser Ala Thr
485 490 495
Thr Ala Leu Ala Val Thr Leu Ser Val Gly Leu Val Ala Cys Val Gly
500 505 510
Val Ile Gly Ala Thr Thr Arg Ala Ser Val Phe Gly Ser Met Glu Ser
515 520 525
Thr Ile Lys Ser Pro Tyr Val Leu Asp Ser Ile Gly Gly Thr Met Ile
530 535 540
Pro Gly Gln Pro Ala Gly Gly Ser Arg Ser Leu Ser Met Ser Ala Ala
545 550 555 560
Val Ala Gln Glu Ile Ala Gln Thr Arg Gly Val Gly Lys Val Gly Thr
565 570 575
Leu Met Thr Gly Ser Val Gln Val Asn Gly Trp Asp Asn Glu Asn Thr
580 585 590
Thr Ile Phe Asp Gly Asp Leu Ser Gln Phe Leu Asp Leu Ala Val Arg
595 600 605
Ser Gly Asp Ala Phe Asp Asp Glu Thr Pro Gly Val Met Ile Ser Thr
610 615 620
Thr Tyr Ala Asp Gln Ser Asp Leu Glu Val Gly Asp Thr Val Thr Val
625 630 635 640
Asn Pro Tyr Gly Ser Asp Asp Gly Ile Arg Val Pro Ile Thr Gly Ile
645 650 655
Tyr Ala Glu Thr Asn Leu Val Gly His Leu Met Val Asn Ala Ala Ala
660 665 670
Thr Asn Arg Val Leu Thr Ser Ala Asp Thr Tyr His Arg Ser Gln Ile
675 680 685
Phe Val Asn Gly Asp Gly Ser Thr Thr Asn Glu Glu Leu Arg Asp Ile
690 695 700
Leu Val Asp Ala Val Ala Pro Phe Leu Ile Val Gln Val Lys Ser Lys
705 710 715 720
Asp Glu Phe Arg Gly Ser Leu Gly Thr Gln Ile Asn Gln Leu Leu Gly
725 730 735
Ile Ile Tyr Gly Leu Leu Ala Leu Ala Val Ile Ile Ala Val Leu Gly
740 745 750
Ile Val Asn Thr Leu Phe Leu Ser Ile Ser Glu Arg Thr Arg Glu Ile
755 760 765
Gly Ile Leu Arg Ala Thr Gly Val Gln Arg Gly Gln Ile Arg Arg Met
770 775 780
Ile Thr Leu Glu Ser Val Ile Leu Ser Ile His Gly Ala Ile His Gly
785 790 795 800
Leu Leu Leu Gly Thr Phe Ile Gly Trp Ala Ile Val Ser Cys Leu Arg
805 810 815
Thr Arg Gly Met Ala Pro Val Glu Phe Pro Trp Thr Gln Ile Gly Leu
820 825 830
Met Leu Ile Ser Ala Ile Ile Ile Gly Gly Ile Ala Ala Leu Ile Pro
835 840 845
Ala Asn Arg Ala Ser Arg Ile Ser Pro Leu Glu Ala Ile Asn
850 855 860
<210>309
<211>772
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(742)
<223>RXA02570
<400>309
ccattgttat gctcattgtg tttgtggtgg tcaagtcgct acccaagcgc accactaggg 60
cattggttcc gcagcgggtt ccggaggacg tcgcttaaac atg aat cct ttg aca 115
Met Asn Pro Leu Thr
1 5
tgg atc att ggc gca ttc agc atg tgg atc gtg gtg ctg ggc gtt aat 163
Trp Ile Ile Gly Ala Phe Ser Met Trp Ile Val Val Leu Gly Val Asn
10 15 20
aag ctt ggt tta agc atc gca gtg atc atc atc gcg cag gtc gtg gcg 211
Lys Leu Gly Leu Ser Ile Ala Val Ile Ile Ile Ala Gln Val Val Ala
25 30 35
atg att cgg gtg cgc aat gta tct gtg ttg gct tca aca gca ttg tta 259
Met Ile Arg Val Arg Asn Val Ser Val Leu Ala Ser Thr Ala Leu Leu
40 45 50
tcg gtt cct gca ttg gcc tcg atg gcg ctg att cac atg ccg tat tct 307
Ser Val Pro Ala Leu Ala Ser Met Ala Leu Ile His Met Pro Tyr Ser
55 60 65
tcc gac ggc tgg ttg att gct ctt acc ttg acg gct cgt ttt agt gcg 355
Ser Asp Gly Trp Leu Ile Ala Leu Thr Leu Thr Ala Arg Phe Ser Ala
70 75 80 85
ttg atg tct att ttc ctc ctt gca gca aca gcg att act att cct gag 403
Leu Met Ser Ile Phe Leu Leu Ala Ala Thr Ala Ile Thr Ile Pro Glu
90 95 100
ctg gtg aaa tcc cta tat cgt tgg ccc aag ctg gcg tat atc gtg ggt 451
Leu Val Lys Ser Leu Tyr Arg Trp Pro Lys Leu Ala Tyr Ile Val Gly
105 110 115
tct gca ttg cag atg att ccg cag ggt aaa cag acc ttg gcg ttg gtt 499
Ser Ala Leu Gln Met Ile Pro Gln Gly Lys Gln Thr Leu Ala Leu Val
120 125 130
cgt gat gcc aat gct ttg cgc ggg cgc agc gtt aaa ggt ccc gtg cgc 547
Arg Asp Ala Asn Ala Leu Arg Gly Arg Ser Val Lys Gly Pro Val Arg
135 140 145
gcg gtg aaa tat gtg ggt ttg ccc ctg att aca cat tta ctt agt gca 595
Ala Val Lys Tyr Val Gly Leu Pro Leu Ile Thr His Leu Leu Ser Ala
150 155 160 165
ggt gcc gcg cga gcg att ccc ttg gag gtc gca ggc ctg gac agg ccg 643
Gly Ala Ala Arg Ala Ile Pro Leu Glu Val Ala Gly Leu Asp Arg Pro
170 175 180
ggg ccg cgt acg gtg ttg gtt gag gtg gtg gag ggg cgc gtc gaa aag 691
Gly Pro Arg Thr Val Leu Val Glu Val Val Glu Gly Arg Val Glu Lys
185 190 195
cat tgt cgc tgg ttg ttg ccg ctt ttg gca gtc ggg atg gcg tgg tgg 739
His Cys Arg Trp Leu Leu Pro Leu Leu Ala Val Gly Met Ala Trp Trp
200 205 210
ctc taactcaaat cgtcggaccg tccggctccg 772
Leu
<210>310
<211>214
<212>PRT
<213>谷氨酸棒状杆菌
<400>310
Met Asn Pro Leu Thr Trp Ile Ile Gly Ala Phe Ser Met Trp Ile Val
1 5 10 15
Val Leu Gly Val Asn Lys Leu Gly Leu Ser Ile Ala Val Ile Ile Ile
20 25 30
Ala Gln Val Val Ala MetIle Arg Val Arg Asn Val Ser Val Leu Ala
35 40 45
Ser Thr Ala Leu Leu Ser Val Pro Ala Leu Ala Ser Met Ala Leu Ile
50 55 60
His Met Pro Tyr Ser Ser Asp Gly Trp Leu Ile Ala Leu Thr Leu Thr
65 70 75 80
Ala Arg Phe Ser Ala Leu Met Ser Ile Phe Leu Leu Ala Ala Thr Ala
85 90 95
Ile Thr Ile Pro Glu Leu Val Lys Ser Leu Tyr Arg Trp Pro Lys Leu
100 105 110
Ala Tyr Ile Val Gly Ser Ala Leu Gln Met Ile Pro Gln Gly Lys Gln
115 120 125
Thr Leu Ala Leu Val Arg Asp Ala Asn Ala Leu Arg Gly Arg Ser Val
130 135 140
Lys Gly Pro Val Arg Ala Val Lys Tyr Val Gly Leu Pro Leu Ile Thr
145 150 155 160
His Leu Leu Ser Ala Gly Ala Ala Arg Ala Ile Pro Leu Glu Val Ala
165 170 175
Gly Leu Asp Arg Pro Gly Pro Arg Thr Val Leu Val Glu Val Val Glu
180 185 190
Gly Arg Val Glu Lys His Cys Arg Trp Leu Leu Pro Leu Leu Ala Val
195 200 205
Gly Met Ala Trp Trp Leu
210
<210>311
<211>1960
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1930)
<223>RXA02591
<400>311
atgtgtccgt tgtctcacct aaagttttaa ctagttctgt atctgaaagc tacgctaggg 60
ggcgagaact ctgtcgaatg acacaaaatc tggagaagta atg act act gct gca 115
Met Thr Thr Ala Ala
1 5
atc agg ggc ctt cag ggc gag gcg ccg acc aag aat aag gaa ctg ctg 163
Ile Arg Gly Leu Gln Gly Glu Ala Pro Thr Lys Asn Lys Glu Leu Leu
10 15 20
aac tgg atc gca gac gcc gtc gag ctc ttc cag cct gag gct gtt gtg 211
Asn Trp Ile Ala Asp Ala Val Glu Leu Phe Gln Pro Glu Ala Val Val
25 30 35
ttc gtt gat gga tcc cag gct gag tgg gat cgc atg gcg gag gat ctt 259
Phe Val Asp Gly Ser Gln Ala Glu Trp Asp Arg Met Ala Glu Asp Leu
40 45 50
gtt gaa gcc ggt acc ctc atc aag ctc aac gag gaa aag cgt ccg aac 307
Val Glu Ala Gly Thr Leu Ile Lys Leu Asn Glu Glu Lys Arg Pro Asn
55 60 65
agc tac cta gct cgt tcc aac cca tct gac gtt gcg cgc gtt gag tcc 355
Ser Tyr Leu Ala Arg Ser Asn Pro Ser Asp Val Ala Arg Val Glu Ser
70 75 80 85
cgc acc ttc atc tgc tcc gag aag gaa gaa gat gct ggc cca acc aac 403
Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala Gly Pro Thr Asn
90 95 100
aac tgg gct cca cca cag gca atg aag gac gaa atg tcc aag cat tac 451
Asn Trp Ala Pro Pro Gln Ala Met Lys Asp Glu Met Ser Lys His Tyr
105 110 115
gct ggt tcc atg aag ggg cgc acc atg tac gtc gtg cct ttc tgc atg 499
Ala Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val Pro Phe Cys Met
120 125 130
ggt cca atc agc gat ccg gac cct aag ctt ggt gtg cag ctc act gac 547
Gly Pro Ile Ser Asp Pro Asp Pro Lys Leu Gly Val Gln Leu Thr Asp
135 140 145
tcc gag tac gtt gtc atg tcc atg cgc atc atg acc cgc atg ggt att 595
Ser Glu Tyr Val Val Met Ser Met Arg Ile Met Thr Arg Met Gly Ile
150 155 160 165
gaa gcg ctg gac aag atc ggc gcg aac ggc agc ttc gtc agg tgc ctc 643
Glu Ala Leu Asp Lys Ile Gly Ala Asn Gly Ser Phe Val Arg Cys Leu
170 175 180
cac tcc gtt ggt gct cct ttg gag cca ggc cag gaa gac gtt gca tgg 691
His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu Asp Val Ala Trp
185 190 195
cct tgc aac gac acc aag tac atc acc cag ttc cca gag acc aag gaa 739
Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro Glu Thr Lys Glu
200 205 210
att tgg tcc tac ggt tcc ggc tac ggc gga aac gca atc ctg gca aag 787
Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala Ile Leu Ala Lys
215 220 225
aag tgc tac gca ctg cgt atc gca tct gtc atg gct cgc gaa gaa gga 835
Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala Arg Glu Glu Gly
230 235 240 245
tgg atg gct gag cac atg ctc atc ctg aag ctg atc aac cca gag ggc 883
Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Ile Asn Pro Glu Gly
250 255 260
aag gcg tac cac atc gca gca gca ttc cca tct gct tgt ggc aag acc 931
Lys Ala Tyr His Ile Ala Ala Ala Phe Pro Ser Ala Cys Gly Lys Thr
265 270 275
aac ctc gcc atg atc act cca acc atc cca ggc tgg acc gct cag gtt 979
Asn Leu Ala Met Ile Thr Pro Thr Ile Pro Gly Trp Thr Ala Gln Val
280 285 290
gtt ggc gac gac atc gct tgg ctg aag ctg cgc gag gac ggc ctc tac 1027
Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu Asp Gly Leu Tyr
295 300 305
gca gtt aac cca gaa aat ggt ttc ttc ggt gtt gct cca ggc acc aac 1075
Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala Pro Gly Thr Asn
310 315 320 325
tac gca tcc aac cca atc gcg atg aag acc atg gaa cca ggc aac acc 1123
Tyr Ala Ser Asn Pro Ile Ala Met Lys Thr Met Glu Pro Gly Asn Thr
330 335 340
ctg ttc acc aac gtg gca ctc acc gac gac ggc gac atc tgg tgg gaa 1171
Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp Ile Trp Trp Glu
345 350 355
ggc atg gac ggc gac gcc cca gct cac ctc att gac tgg atg ggc aac 1219
Gly Met Asp Gly Asp Ala Pro Ala His Leu Ile Asp Trp Met Gly Asn
360 365 370
gac tgg acc cca gag tcc gac gaa aac gct gct cac cct aac tcc cgt 1267
Asp Trp Thr Pro Glu Ser Asp Glu Asn Ala Ala His Pro Asn Ser Arg
375 380 385
tac tgc gta gca atc gac cag tcc cca gca gca gca cct gag ttc aac 1315
Tyr Cys Val Ala Ile Asp Gln Ser Pro Ala Ala Ala Pro Glu Phe Asn
390 395 400 405
gac tgg gaa ggc gtc aag atc gac gca atc ctc ttc ggt gga cgt cgc 1363
Asp Trp Glu Gly Val Lys Ile Asp Ala Ile Leu Phe Gly Gly Arg Arg
410 415 420
gca gac acc gtc cca ctg gtt acc cag acc tac gac tgg gag cac ggc 1411
Ala Asp Thr Val Pro Leu Val Thr Gln Thr Tyr Asp Trp Glu His Gly
425 430 435
acc atg gtt ggt gca ctg ctc gca tcc ggt cag acc gca gct tcc gca 1459
Thr Met Val Gly Ala Leu Leu Ala Ser Gly Gln Thr Ala Ala Ser Ala
440 445 450
gaa gca aag gtc ggc aca ctc cgc cac gac cca atg gca atg ctc cca 1507
Glu Ala Lys Val Gly Thr Leu Arg His Asp Pro Met Ala Met Leu Pro
455 460 465
ttc att ggc tac aac gct ggt gaa tac ctg cag aac tgg att gac atg 1555
Phe Ile Gly Tyr Asn Ala Gly Glu Tyr Leu Gln Asn Trp Ile Asp Met
470 475 480 485
ggt aac aag ggt ggc gac aag atg cca tcc atc ttc ctg gtc aac tgg 1603
Gly Asn Lys Gly Gly Asp Lys Met Pro Ser Ile Phe Leu Val Asn Trp
490 495 500
ttc cgc cgt ggc gaa gat gga cgc ttc ctg tgg cct ggc ttc ggc gac 1651
Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro Gly Phe Gly Asp
505 510 515
aac tct cgc gtt ctg aag tgg gtc atc gac cgc atc gaa ggc cac gtt 1699
Asn Ser Arg Val Leu Lys Trp Val Ile Asp Arg Ile Glu Gly His Val
520 525 530
ggc gca gac gag acc gtt gtt gga cac acc gct aag gcc gaa gac ctc 1747
Gly Ala Asp Glu Thr Val Val Gly His Thr Ala Lys Ala Glu Asp Leu
535 540 545
gac ctc gac ggc ctc gac acc cca att gag gat gtc aag gaa gca ctg 1795
Asp Leu Asp Gly Leu Asp Thr Pro Ile Glu Asp Val Lys Glu Ala Leu
550 555 560 565
acc gct cct gca gag cag tgg gca aac gac gtt gaa gac aac gcc gag 1843
Thr Ala Pro Ala Glu Gln Trp Ala Asn Asp Val Glu Asp Asn Ala Glu
570 575 580
tac ctc act ttc ctc gga cca cgt gtt cct gca gag gtt cac agc cag 1891
Tyr Leu Thr Phe Leu Gly Pro Arg Val Pro Ala Glu Val His Ser Gln
585 590 595
ttc gat gct ctg aag gcc cgc att tca gca gct cac gct taaagttcac 1940
Phe Asp Ala Leu Lys Ala Arg Ile Ser Ala Ala His Ala
600 605 610
gcttaagaac tgctaaataa 1960
<210>312
<211>610
<212>PRT
<213>谷氨酸棒状杆菌
<400>312
Met Thr Thr Ala Ala Ile Arg Gly Leu Gln Gly Glu Ala Pro Thr Lys
1 5 10 15
Asn Lys Glu Leu Leu Asn Trp Ile Ala Asp Ala Val Glu Leu Phe Gln
20 25 30
Pro Glu Ala Val Val Phe Val Asp Gly Ser Gln Ala Glu Trp Asp Arg
35 40 45
Met Ala Glu Asp Leu Val Glu Ala Gly Thr Leu Ile Lys Leu Asn Glu
50 55 60
Glu Lys Arg Pro Asn Ser Tyr Leu Ala Arg Ser Asn Pro Ser Asp Val
65 70 75 80
Ala Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp
85 90 95
Ala Gly Pro Thr Asn Asr Trp Ala Pro Pro Gln Ala Met Lys Asp Glu
100 105 110
Met Ser Lys His Tyr Ala Gly Ser Met Lys Gly Arg Thr Met Tyr Val
115 120 125
Val Pro Phe Cys Met Gly Pro Ile Ser Asp Pro Asp Pro Lys Leu Gly
130 135 140
Val Gln Leu Thr Asp Ser Glu Tyr Val Val Met Ser Met Arg Ile Met
145 150 155 160
Thr Arg Met Gly Ile Glu Ala Leu Asp Lys Ile Gly Ala Asn Gly Ser
165 170 175
Phe Val Arg Cys Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln
180 185 190
Glu Asp Val Ala Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe
195 200 205
Pro Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn
210 215 220
Ala Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met
225 230 235 240
Ala Arg Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu
245 250 255
Ile Asn Pro Glu Gly Lys Ala Tyr His Ile Ala Ala Ala Phe Pro Ser
260 265 270
Ala Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Pro Gly
275 280 285
Trp Thr Ala Gln Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg
290 295 300
Glu Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val
305 310 315 320
Ala Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Lys Thr Met
325 330 335
Glu Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly
340 345 350
Asp Ile Trp Trp Glu Gly Met Asp Gly Asp Ala Pro Ala His Leu Ile
355 360 365
Asp Trp Met Gly Asn Asp Trp Thr Pro Glu Ser Asp Glu Asn Ala Ala
370 375 380
His Pro Asn Ser Arg Tyr Cys Val Ala Ile Asp Gln Ser Pro Ala Ala
385 390 395 400
Ala Pro Glu Phe Asn Asp Trp Glu Gly Val Lys Ile Asp Ala Ile Leu
405 410 415
Phe Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Thr Tyr
420 425 430
Asp Trp Glu His Gly Thr Met Val Gly Ala Leu Leu Ala Ser Gly Gln
435 440 445
Thr Ala Ala Ser Ala Glu Ala Lys Val Gly Thr Leu Arg His Asp Pro
450 455 460
Met Ala Met Leu Pro Phe Ile Gly Tyr Asn Ala Gly Glu Tyr Leu Gln
465 470 475 480
Asn Trp Ile Asp Met Gly Asn Lys Gly Gly Asp Lys Met Pro Ser Ile
485 490 495
Phe Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp
500 505 510
Pro Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Val Ile Asp Arg
515 520 525
Ile Glu Gly His Val Gly Ala Asp Glu Thr Val Val Gly His Thr Ala
530 535 540
Lys Ala Glu Asp Leu Asp Leu Asp Gly Leu Asp Thr Pro Ile Glu Asp
545 550 555 560
Val Lys Glu Ala Leu Thr Ala Pro Ala Glu Gln Trp Ala Asn Asp Val
565 570 575
Glu Asp Asn Ala Glu Tyr Leu Thr Phe Leu Gly Pro Arg Val Pro Ala
580 585 590
Glu Val His Ser Gln Phe Asp Ala Leu Lys Ala Arg Ile Ser Ala Ala
595 600 605
His Ala
610
<210>313
<211>2206
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2176)
<223>RXA02604
<400>313
tctgcatcgt gatggctgtg tacatcatgc cgatgttttg aatatttacc aatgaacatg 60
ctctgaccag catgtccatt aacgtttgag gccacatgcg gtg cta gca tgt ggc 115
Val Leu Ala Cys Gly
1 5
ctc atg acg ttt agc ccc cag cgt ccg gag ttt gag acc ggt aag cag 163
Leu Met Thr Phe Ser Pro Gln Arg Pro Glu Phe Glu Thr Gly Lys Gln
10 15 20
cca gat cca gaa act gaa cac gca ggt gac ttc ttt gaa gaa acc tca 211
Pro Asp Pro Glu Thr Glu His Ala Gly Asp Phe Phe Glu Glu Thr Ser
25 30 35
agc agt gct cct cgc gcc gca tct aac ggt tct tct ggt ccg aac tac 259
Ser Ser Ala Pro Arg Ala Ala Ser Asn Gly Ser Ser Gly Pro Asn Tyr
40 45 50
acc ctc att aca acg ttt cta gcg gcc ctt act gct ggc atc ttt gct 307
Thr Leu Ile Thr Thr Phe Leu Ala Ala Leu Thr Ala Gly Ile Phe Ala
55 60 65
ttc tgg gca ggc tgg acc cgc aaa tgg atc agc gac gac gga ctg atc 355
Phe Trp Ala Gly Trp Thr Arg Lys Trp Ile Ser Asp Asp Gly Leu Ile
70 75 80 85
gtc cta cgc acc gtc cga aac ctc ctg gct gga aac ggg cca gta ttc 403
Val Leu Arg Thr Val Arg Asn Leu Leu Ala Gly Asn Gly Pro Val Phe
90 95 100
aac gct ggc gaa cgc gtc gaa gcc aac aca tct act ctg tgg caa tac 451
Asn Ala Gly Glu Arg Val Glu Ala Asn Thr Ser Thr Leu Trp Gln Tyr
105 110 115
tgc atc tat ctg gtt gcc tta gta act gac tat cgc ctc gaa gat att 499
Cys Ile Tyr Leu Val Ala Leu Val Thr Asp Tyr Arg Leu Glu Asp Ile
120 125 130
gct ctg tgg ctt gcg ctg ctg ttc acc acc gca gcg tcc atc atc ggt 547
Ala Leu Trp Leu Ala Leu Leu Phe Thr Thr Ala Ala Ser Ile Ile Gly
135 140 145
gtc ctg ggt acc gcg cat ctc cac cgc aaa cgc att gcc gta ttg ctt 595
Val Leu Gly Thr Ala His Leu His Arg Lys Arg Ile Ala Val Leu Leu
150 155 160 165
cct gca ggc gtg atc ggc tac ttc agc ctt tcc ccg gcg cga gac ttt 643
Pro Ala Gly Val Ile Gly Tyr Phe Ser Leu Ser Pro Ala Arg Asp Phe
170 175 180
gcc act tcc gga ttg gag tgg ggc cta tct ttg atg tgg att tcc atc 691
Ala Thr Ser Gly Leu Glu Trp Gly Leu Ser Leu Met Trp Ile Ser Ile
185 190 195
caa tgg ctg ctg ctg gtg ttg tgg gcg act tcg ggc aag acc tcg ggc 739
Gln Trp Leu Leu Leu Val Leu Trp Ala Thr Ser Gly Lys Thr Ser Gly
200 205 210
aag aag gct tcg ggc gca aaa act tca aat cct atc gtt aat gcc ggt 787
Lys Lys Ala Ser Gly Ala Lys Thr Ser Asn Pro Ile Val Asn Ala Gly
215 220 225
gca ata acc tat gct ttg gcc ttt tgg tca ggc ttg agc tgg ctg gtt 835
Ala Ile Thr Tyr Ala Leu Ala Phe Trp Ser Gly Leu Ser Trp Leu Val
230 235 240 245
cgc cca gaa ctg gcg atg tat ggc ggt ttg act gga gtg ttg ctg ctg 883
Arg Pro Glu Leu Ala Met Tyr Gly Gly Leu Thr Gly Val Leu Leu Leu
250 255 260
ctt act gcg cca cga tgg cgg gta gtt ttg ggg atc ctg gtg gcg gct 931
Leu Thr Ala Pro Arg Trp Arg Val Val Leu Gly Ile Leu Val Ala Ala
265 270 275
ttg cct ctt cca gct gcg tac caa atc ttc cgc atg ggt tat tac ggg 979
Leu Pro Leu Pro Ala Ala Tyr Gln Ile Phe Arg Met Gly Tyr Tyr Gly
280 285 290
ctg atg gtg ccg cac acg gct gta gcg aaa tca gcc tca gat gcg gtg 1027
Leu Met Val Pro His Thr Ala Val Ala Lys Ser Ala Ser Asp Ala Val
295 300 305
tgg ggg act ggt tgg gaa tat gtt gag gat ttc acg ggg cct tac aac 1075
Trp Gly Thr Gly Trp Glu Tyr Val Glu Asp Phe Thr Gly Pro Tyr Asn
310 315 320 325
ctg tgg ctc ggt ttg gcc ttg ctg ttg gcc gca ggc gcg ttg aca gtg 1123
Leu Trp Leu Gly Leu Ala Leu Leu Leu Ala Ala Gly Ala Leu Thr Val
330 335 340
tgg aaa act gac aag cac tta gcg ata ccg aag ggg cgg ctg ggg ctg 1171
Trp Lys Thr Asp Lys His Leu Ala Ile Pro Lys Gly Arg Leu Gly Leu
345 350 355
cgc act ccg ggt atg gct ata gcg ttg ctg gtt atc tgt gcg ctc gtc 1219
Arg Thr Pro Gly Met Ala Ile Ala Leu Leu Val Ile Cys Ala Leu Val
360 365 370
cac ttc ctt tac gtt atc cgt gtt ggt ggc gac ttc atg cat gga cgc 1267
His Phe Leu Tyr Val Ile Arg Val Gly Gly Asp Phe Met His Gly Arg
375 380 385
atg ctg ctc ctt cca ctt ttt gcc att ctg ctg cct gtt tct gtc att 1315
Met Leu Leu Leu Pro Leu Phe Ala Ile Leu Leu Pro Val Ser Val Ile
390 395 400 405
ccg gtc aat gtt gtt gat cga ggt tgg cag gat ttg gtt gcg ctg gtt 1363
Pro Val Asn Val Val Asp Arg Gly Trp Gln Asp Leu Val Ala Leu Val
410 415 420
ctc gtt ttc tct acg tgg gtg tgg tcc act gtg gtt ttt gtg cag ggg 1411
Leu Val Phe Ser Thr Trp Val Trp Ser Thr Val Val Phe Val Gln Gly
425 430 435
cac caa tgg gaa aat acc ggc cag cat gtg gtt gat gag cgt gat ttt 1459
His Gln Trp Glu Asn Thr Gly Gln His Val Val Asp Glu Arg Asp Phe
440 445 450
tgg att gat ttc acc aac cga gat gaa gat cat cct ccg ctt tat gca 1507
Trp Ile Asp Phe Thr Asn Arg Asp Glu Asp His Pro Pro Leu Tyr Ala
455 460 465
gag gat ttc ctc act gtt gat tcc atg aat gat tac gca gag gtt atg 1555
Glu Asp Phe Leu Thr Val Asp Ser Met Asn Asp Tyr Ala Glu Val Met
470 475 480 485
cgc gat cag acg ttg gtt aat cca aca ggc cag caa ctc aat att ctg 1603
Arg Asp Gln Thr Leu Val Asn Pro Thr Gly Gln Gln Leu Asn Ile Leu
490 495 500
gcc agc agt gac ccg acc act tat tcg tgg atc acc aca cct cgc gtg 1651
Ala Ser Ser Asp Pro Thr Thr Tyr Ser Trp Ile Thr Thr Pro Arg Val
505 510 515
gag ggg gtt gaa gcc ggt gat ttg gct aac ctc tcg cca act gtt ttc 1699
Glu Gly Val Glu Ala Gly Asp Leu Ala Asn Leu Ser Pro Thr Val Phe
520 525 530
cat gtg aac ctc ggc atg acc tcc atg aac gca ccg ctc aac gtg cgt 1747
His Val Asn Leu Gly Met Thr Ser Met Asn Ala Pro Leu Asn Val Arg
535 540 545
gtg aca gac ctg att ggt ttg gca acg cca ctg gca gcc cgc cag cca 1795
Val Thr Asp Leu Ile Gly Leu Ala Thr Pro Leu Ala Ala Arg Gln Pro
550 555 560 565
cgc att gaa ggt ggt cga atc ggc cac gat aaa ttg atg gac ttg gaa 1843
Arg Ile Glu Gly Gly Arg Ile Gly His Asp Lys Leu Met Asp Leu Glu
570 575 580
tgg cag gtc gcg gaa tcc gcc act ccg ctg gcg tac aca ccg ggt tgg 1891
Trp Gln Val Ala Glu Ser Ala Thr Pro Leu Ala Tyr Thr Pro Gly Trp
585 590 595
ttg gat act caa aag act tat gag gcc cgc cag gcg cta cgc cac cca 1939
Leu Asp Thr Gln Lys Thr Tyr Glu Ala Arg Gln Ala Leu Arg His Pro
600 605 610
gaa ttg gtt cac ctt ttc cag act tac cgt gag cca atg tcc tac cac 1987
Glu Leu Val His Leu Phe Gln Thr Tyr Arg Glu Pro Met Ser Tyr His
615 620 625
cgg ttt gtg gac aat att aaa tac gca ctc act acc gga aga aca ctg 2035
Arg Phe Val Asp Asn Ile Lys Tyr Ala Leu Thr Thr Gly Arg Thr Leu
630 635 640 645
gaa att tca gat aat cct gaa gat ctt ttg aaa gaa ttt aac ccg acc 2083
Glu Ile Ser Asp Asn Pro Glu Asp Leu Leu Lys Glu Phe Asn Pro Thr
650 655 660
cct gca gag att cag gac ggg tta gaa act att gct tgg cct ggg gaa 2131
Pro Ala Glu Ile Gln Asp Gly Leu Glu Thr Ile Ala Trp Pro Gly Glu
665 670 675
att aaa ctt gat gaa cct cgc gga gaa cct tta tat agc tct cag 2176
Ile Lys Leu Asp Glu Pro Arg Gly Glu Pro Leu Tyr Ser Ser Gln
680 685 690
taaacactta ttcgcaaatc attgggctgt 2206
<210>314
<211>692
<212>PRT
<213>谷氨酸棒状杆菌
<400>314
Val Leu Ala Cys Gly Leu Met Thr Phe Ser Pro Gln Arg Pro Glu Phe
1 5 10 15
Glu Thr Gly Lys Gln Pro Asp Pro Glu Thr Glu His Ala Gly Asp Phe
20 25 30
Phe Glu Glu Thr Ser Ser Ser Ala Pro Arg Ala Ala Ser Asn Gly Ser
35 40 45
Ser Gly Pro Asn Tyr Thr Leu Ile Thr Thr Phe Leu Ala Ala Leu Thr
50 55 60
Ala Gly Ile Phe Ala Phe Trp Ala Gly Trp Thr Arg Lys Trp Ile Ser
65 70 75 80
Asp Asp Gly Leu Ile Val Leu Arg Thr Val Arg Asn Leu Leu Ala Gly
85 90 95
Asn Gly Pro Val Phe Asn Ala Gly Glu Arg Val Glu Ala Asn Thr Ser
100 105 110
Thr Leu Trp Gln Tyr Cys Ile Tyr Leu Val Ala Leu Val Thr Asp Tyr
115 120 125
Arg Leu Glu Asp Ile Ala Leu Trp Leu Ala Leu Leu Phe Thr Thr Ala
130 135 140
Ala Ser Ile Ile Gly Val Leu Gly Thr Ala His Leu His Arg Lys Arg
145 150 155 160
Ile Ala Val Leu Leu Pro Ala Gly Val Ile Gly Tyr Phe Ser Leu Ser
165 170 175
Pro Ala Arg Asp Phe Ala Thr Ser Gly Leu Glu Trp Gly Leu Ser Leu
180 185 190
Met Trp Ile Ser Ile Gln Trp Leu Leu Leu Val Leu Trp Ala Thr Ser
195 200 205
Gly Lys Thr Ser Gly Lys Lys Ala Ser Gly Ala Lys Thr Ser Asn Pro
210 215 220
Ile Val Asn Ala Gly Ala Ile Thr Tyr Ala Leu Ala Phe Trp Ser Gly
225 230 235 240
Leu Ser Trp Leu Val Arg Pro Glu Leu Ala Met Tyr Gly Gly Leu Thr
245 250 255
Gly Val Leu Leu Leu Leu Thr Ala Pro Arg Trp Arg Val Val Leu Gly
260 265 270
Ile Leu Val Ala Ala Leu Pro Leu Pro Ala Ala Tyr Gln Ile Phe Arg
275 280 285
Met Gly Tyr Tyr Gly Leu Met Val Pro His Thr Ala Val Ala Lys Ser
290 295 300
Ala Ser Asp Ala Val Trp Gly Thr Gly Trp Glu Tyr Val Glu Asp Phe
305 310 315 320
Thr Gly Pro Tyr Asn Leu Trp Leu Gly Leu Ala Leu Leu Leu Ala Ala
325 330 335
Gly Ala Leu Thr Val Trp Lys Thr Asp Lys His Leu Ala Ile Pro Lys
340 345 350
Gly Arg Leu Gly Leu Arg Thr Pro Gly Met Ala Ile Ala Leu Leu Val
355 360 365
Ile Cys Ala Leu Val His Phe Leu Tyr Val Ile Arg Val Gly Gly Asp
370 375 380
Phe Met His Gly Arg Met Leu Leu Leu Pro Leu Phe Ala Ile Leu Leu
385 390 395 400
Pro Val Ser Val Ile Pro Val Asn Val Val Asp Arg Gly Trp Gln Asp
405 410 415
Leu Val Ala Leu Val Leu Val Phe Ser Thr Trp Val Trp Ser Thr Val
420 425 430
Val Phe Val Gln Gly His Gln Trp Glu Asn Thr Gly Gln His Val Val
435 440 445
Asp Glu Arg Asp Phe Trp Ile Asp Phe Thr Asn Arg Asp Glu Asp His
450 455 460
Pro Pro Leu Tyr Ala Glu Asp Phe Leu Thr Val Asp Ser Met Asn Asp
465 470 475 480
Tyr Ala Glu Val Met Arg Asp Gln Thr Leu Val Asn Pro Thr Gly Gln
485 490 495
Gln Leu Asn Ile Leu Ala Ser Ser Asp Pro Thr Thr Tyr Ser Trp Ile
500 505 510
Thr Thr Pro Arg Val Glu Gly Val Glu Ala Gly Asp Leu Ala Asn Leu
515 520 525
Ser Pro Thr Val Phe His Val Asn Leu Gly Met Thr Ser Met Asn Ala
530 535 540
Pro Leu Asn Val Arg Val Thr Asp Leu Ile Gly Leu Ala Thr Pro Leu
545 550 555 560
Ala Ala Arg Gln Pro Arg Ile Glu Gly Gly Arg Ile Gly His Asp Lys
565 570 575
Leu Met Asp Leu Glu Trp Gln Val Ala Glu Ser Ala Thr Pro Leu Ala
580 585 590
Tyr Thr Pro Gly Trp Leu Asp Thr Gln Lys Thr Tyr Glu Ala Arg Gln
595 600 605
Ala Leu Arg His Pro Glu Leu Val His Leu Phe Gln Thr Tyr Arg Glu
610 615 620
Pro Met Ser Tyr His Arg Phe Val Asp Asn Ile Lys Tyr Ala Leu Thr
625 630 635 640
Thr Gly Arg Thr Leu Glu Ile Ser Asp Asn Pro Glu Asp Leu Leu Lys
645 650 655
Glu Phe Asn Pro Thr Pro Ala Glu Ile Gln Asp Gly Leu Glu Thr Ile
660 665 670
Ala Trp Pro Gly Glu Ile Lys Leu Asp Glu Pro Arg Gly Glu Pro Leu
675 680 685
Tyr Ser Ser Gln
690
<210>315
<211>1048
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1018)
<223>RXA02678
<400>315
ctgtgggcgc gcgaacaggg cgtcgacatg ctcgccacca attatccgga ccgtgcggcg 60
gagcttttga acgcacatcc caagcccgcc atgtacgcta atg cgc atg gca aag 115
Met Arg Met Ala Lys
1 5
aag act aag aag aat gaa cag ctg ccg gag ggc atg agc cgt cgt cag 163
Lys Thr Lys Lys Asn Glu Gln Leu Pro Glu Gly Met Ser Arg Arg Gln
10 15 20
gca aaa ctt gca gcc cgc gcg gct gaa cgt gca gca ctt gag cgt gaa 211
Ala Lys Leu Ala Ala Arg Ala Ala Glu Arg Ala Ala Leu Glu Arg Glu
25 30 35
cct cgc cca ttc gag ggc ctg gca atg gag tca cag ctc gtt gca ctg 259
Pro Arg Pro Phe Glu Gly Leu Ala Met Glu Ser Gln Leu Val Ala Leu
40 45 50
cag gaa ttc att cca tct gca acc gca ccc atc acc gtt gca ggc acc 307
Gln Glu Phe Ile Pro Ser Ala Thr Ala Pro Ile Thr Val Ala Gly Thr
55 60 65
gat cgc aag atc acc ctc tgt acc gtg ctt cca ggc gca gct gcg gca 355
Asp Arg Lys Ile Thr Leu Cys Thr Val Leu Pro Gly Ala Ala Ala Ala
70 75 80 85
ctt gtc cgc gaa gaa gca ttc ggc ggc gaa gcg ttt gtt gca atg cag 403
Leu Val Arg Glu Glu Ala Phe Gly Gly Glu Ala Phe Val Ala Met Gln
90 95 100
cag gcc atc cgc tcc aac aac cca agc aag gac ctc gcg ttc gcg ctg 451
Gln Ala Ile Arg Ser Asn Asn Pro Ser Lys Asp Leu Ala Phe Ala Leu
105 110 115
aac tgg gtc atc aac gca aaa gcc ggc gag tca ctg gcc acc gcc aca 499
Asn Trp Val Ile Asn Ala Lys Ala Gly Glu Ser Leu Ala Thr Ala Thr
120 125 130
gcg gac ggc acc cag cca gag cta aag agc ttg ctt aac gac gcc gac 547
Ala Asp Gly Thr Gln Pro Glu Leu Lys Ser Leu Leu Asn Asp Ala Asp
135 140 145
acc ctc gaa atc acc act cac cag gac ttc aac tgg tgg ctg gcg gaa 595
Thr Leu Glu Ile Thr Thr His Gln Asp Phe Asn Trp Trp Leu Ala Glu
150 155 160 165
aac gac aac ctc tcc cca gaa gtt gcg cag cac atg cag gca gcc aat 643
Asn Asp Asn Leu Ser Pro Glu Val Ala Gln His Met Gln Ala Ala Asn
170 175 180
gac tcc atc ctg cct tca cac gaa gtc gaa gca gac gtc cca ggc gct 691
Asp Ser Ile Leu Pro Ser His Glu Val Glu Ala Asp Val Pro Gly Ala
185 190 195
gtt tgg tgg gtc aac cca ggc gga aaa gcg cac atc cgt tgg gtc cgc 739
Val Trp Trp Val Asn Pro Gly Gly Lys Ala His Ile Arg Trp Val Arg
200 205 210
acc gaa aac gaa acc gca ctg ttc aac gct ttg gca cgc atc gca gca 787
Thr Glu Asn Glu Thr Ala Leu Phe Asn Ala Leu Ala Arg Ile Ala Ala
215 220 225
cgt ggc gag ctg aat ctc ggc gaa gaa act aag ttc gct ggt gct ttc 835
Arg Gly Glu Leu Asn Leu Gly Glu Glu Thr Lys Phe Ala Gly Ala Phe
230 235 240 245
cgc acc cac ggc atc gtc gtc cca gtg tgg gat ctc gat cctgag cgt 883
Arg Thr His Gly Ile Val Val Pro Val Trp Asp Leu Asp Pro Glu Arg
250 255 260
cca tcc acc gat tac gcc gat gtt ttg gtt gca ctc aac gag aag atc 931
Pro Ser Thr Asp Tyr Ala Asp Val Leu Val Ala Leu Asn Glu Lys Ile
265 270 275
gtc gct gaa ctg gat aac gat gca cag ctc aac gcc gac gag cgc cgt 979
Val Ala Glu Leu Asp Asn Asp Ala Gln Leu Asn Ala Asp Glu Arg Arg
280 285 290
cag ctg gag aac atc aag tcc cgc cag gtg acc atc cgc taaaatctct 1028
Gln Leu Glu Asn Ile Lys Ser Arg Gln Val Thr Ile Arg
295 300 305
taaaaacact tcaagcgcgt 1048
<210>316
<211>306
<212>PRT
<213>谷氨酸棒状杆菌
<400>316
Met Arg Met Ala Lys Lys Thr Lys Lys Asn Glu Gln Leu Pro Glu Gly
1 5 10 15
Met Ser Arg Arg Gln Ala Lys Leu Ala Ala Arg Ala Ala Glu Arg Ala
20 25 30
Ala Leu Glu Arg Glu Pro Arg Pro Phe Glu Gly Leu Ala Met Glu Ser
35 40 45
Gln Leu Val Ala Leu Gln Glu Phe Ile Pro Ser Ala Thr Ala Pro Ile
50 55 60
Thr Val Ala Gly Thr Asp Arg Lys Ile Thr Leu Cys Thr Val Leu Pro
65 70 75 80
Gly Ala Ala Ala Ala Leu Val Arg Glu Glu Ala Phe Gly Gly Glu Ala
85 90 95
Phe Val Ala Met Gln Gln Ala Ile Arg Ser Asn Asn Pro Ser Lys Asp
100 105 110
Leu Ala Phe Ala Leu Asn Trp Val Ile Asn Ala Lys Ala Gly Glu Ser
115 120 125
Leu Ala Thr Ala Thr Ala Asp Gly Thr Gln Pro Glu Leu Lys Ser Leu
130 135 140
Leu Asn Asp Ala Asp Thr Leu Glu Ile Thr Thr His Gln Asp Phe Asn
145 150 155 160
Trp Trp Leu Ala Glu Asn Asp Asn Leu Ser Pro Glu Val Ala Gln His
165 170 175
Met Gln Ala Ala Asn Asp Ser Ile Leu Pro Ser His Glu Val Glu Ala
180 185 190
Asp Val Pro Gly Ala Val Trp Trp Val Asn Pro Gly Gly Lys Ala His
195 200 205
Ile Arg Trp Val Arg Thr Glu Asn Glu Thr Ala Leu Phe Asn Ala Leu
210 215 220
Ala Arg Ile Ala Ala Arg Gly Glu Leu Asn Leu Gly Glu Glu Thr Lys
225 230 235 240
Phe Ala Gly Ala Phe Arg Thr His Gly Ile Val Val Pro Val Trp Asp
245 250 255
Leu Asp Pro Glu Arg Pro Ser Thr Asp Tyr Ala Asp Val Leu Val Ala
260 265 270
Leu Asn Glu Lys Ile Val Ala Glu Leu Asp Asn Asp Ala Gln Leu Asn
275 280 285
Ala Asp Glu Arg Arg Gln Leu Glu Asn Ile Lys Ser Arg Gln Val Thr
290 295 300
Ile Arg
305
<210>317
<211>1000
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(970)
<223>RXA02683
<400>317
gaagtgtcca gtcaactttt tggttaattg attatcttac ctagagactt ggatacttcc 60
ctccacggat tgctaacgat ccagttttat cgttggactc atg aaa aag atc cac 115
Met Lys Lys Ile His
1 5
atg att cca gcg gcc ctc ctc atc ggt tcc ctt gcg ctt gtt agc tgc 163
Met Ile Pro Ala Ala Leu Leu Ile Gly Ser Leu Ala Leu Val Ser Cys
10 15 20
gcg cct tca caa cct acc cag aac gcg cag acg gtt tct tct gtc atg 211
Ala Pro Ser Gln Pro Thr Gln Asn Ala Gln Thr Val Ser Ser Val Met
25 30 35
acg gaa act cct act tat acc tct tca tct agg tcg gtg gaa tct acc 259
Thr Glu Thr Pro Thr Tyr Thr Ser Ser Ser Arg Ser Val Glu Ser Thr
40 45 50
gcg ggg acg atc gct gcc tcg gag gaa gcc acg atg acg cta ctg gag 307
Ala Gly Thr Ile Ala Ala Ser Glu Glu Ala Thr Met Thr Leu Leu Glu
55 60 65
aat agt tcg ggg gat ccg gga tct gat tct ggg ggt gca cgc tat agc 355
Asn Ser Ser Gly Asp Pro Gly Ser Asp Ser Gly Gly Ala Arg Tyr Ser
70 75 80 85
ctc aat tcg ctc aaa gtt tct gag cag gcc gcg gct aac gcc gtc tta 403
Leu Asn Ser Leu Lys Val Ser Glu Gln Ala Ala Ala Asn Ala Val Leu
90 95 100
aag gcc gtg ctt aac gac gtc tcc tac caa gag ttc gca gac tcc tcc 451
Lys Ala Val Leu Asn Asp Val Ser Tyr Gln Glu Phe Ala Asp Ser Ser
105 110 115
tac tta gaa atc aca gga acg ccc tcc gcc gac gga aca tgg ggc atc 499
Tyr Leu Glu Ile Thr Gly Thr Pro Ser Ala Asp Gly Thr Trp Gly Ile
120 125 130
tca ttt ggc gga cct tcc gaa tcg gcc tcc gtg gaa ttc tcc gac ggc 547
Ser Phe Gly Gly Pro Ser Glu Ser Ala Ser Val Glu Phe Ser Asp Gly
135 140 145
agc atc agc ttc tcc cct gtt gac atg aca gtg ccc gca aca cga ttg 595
Ser Ile Ser Phe Ser Pro Val Asp Met Thr Val Pro Ala Thr Arg Leu
150 155 160 165
cct cag atg ggc gca ttt tat gaa acc ctg acc gaa gaa caa tta ggc 643
Pro Gln Met Gly Ala Phe Tyr Glu Thr Leu Thr Glu Glu Gln Leu Gly
170 175 180
atg ctg gag act ggc ctc gcg gtt tcc acc gtg gat tcg agc caa cag 691
Met Leu Glu Thr Gly Leu Ala Val Ser Thr Val Asp Ser Ser Gln Gln
185 190 195
gaa atg ctt ctg gac ttg gtc tcc aac tcg atc ggc ctg gca gat act 739
Glu Met Leu Leu Asp Leu Val Ser Asn Ser Ile Gly Leu Ala Asp Thr
200 205 210
gaa acc act gcg act gca att agt aaa atc cga gca acg ctt tcc gag 787
Glu Thr Thr Ala Thr Ala Ile Ser Lys Ile Arg Ala Thr Leu Ser Glu
215 220 225
acc tac ctg ttc gga acc ccc gac gga tta acc ttg gcg ctt agt ggc 835
Thr Tyr Leu Phe Gly Thr Pro Asp Gly Leu Thr Leu Ala Leu Ser Gly
230 235 240 245
cca cac gtt gat ttc gaa gtc tcc cac cag ggc agc acc gcg aaa att 883
Pro His Val Asp Phe Glu Val Ser His Gln Gly Ser Thr Ala Lys Ile
250 255 260
act tac cgc gat cca agc acc gac acc tta acc gcc gaa gat cgg gta 931
Thr Tyr Arg Asp Pro Ser Thr Asp Thr Leu Thr Ala Glu Asp Arg Val
265 270 275
gat acc gca agt gtg gct gct gct ccg ccg gag gtt gtt taggcgtttg 980
Asp Thr Ala Ser Val Ala Ala Ala Pro Pro Glu Val Val
280 285 290
ccgagaaatt ttcattgggc 1000
<210>318
<211>290
<212>PRT
<213>谷氨酸棒状杆菌
<400>318
Met Lys Lys Ile His Met Ile Pro Ala Ala Leu Leu Ile Gly Ser Leu
1 5 10 15
Ala Leu Val Ser Cys Ala Pro Ser Gln Pro Thr Gln Asn Ala Gln Thr
20 25 30
Val Ser Ser Val Met Thr Glu Thr Pro Thr Tyr Thr Ser Ser Ser Arg
35 40 45
Ser Val Glu Ser Thr Ala Gly Thr Ile Ala Ala Ser Glu Glu Ala Thr
50 55 60
Met Thr Leu Leu Glu Asn Ser Ser Gly Asp Pro Gly Ser Asp Ser Gly
65 70 75 80
Gly Ala Arg Tyr Ser Leu Asn Ser Leu Lys Val Ser Glu Gln Ala Ala
85 90 95
Ala Asn Ala Val Leu Lys Ala Val Leu Asn Asp Val Ser Tyr Gln Glu
100 105 110
Phe Ala Asp Ser Ser Tyr Leu Glu Ile Thr Gly Thr Pro Ser Ala Asp
115 120 125
Gly Thr Trp Gly Ile Ser Phe Gly Gly Pro Ser Glu Ser Ala Ser Val
130 135 140
Glu Phe Ser Asp Gly Ser Ile Ser Phe Ser Pro Val Asp Met Thr Val
145 150 155 160
Pro Ala Thr Arg Leu Pro Gln Met Gly Ala Phe Tyr Glu Thr Leu Thr
165 170 175
Glu Glu Gln Leu Gly Met Leu Glu Thr Gly Leu Ala Val Ser Thr Val
180 185 190
Asp Ser Ser Gln Gln Glu Met Leu Leu Asp Leu Val Ser Asn Ser Ile
195 200 205
Gly Leu Ala Asp Thr Glu Thr Thr Ala Thr Ala Ile Ser Lys Ile Arg
210 215 220
Ala Thr Leu Ser Glu Thr Tyr Leu Phe Gly Thr Pro Asp Gly Leu Thr
225 230 235 240
Leu Ala Leu Ser Gly Pro His Val Asp Phe Glu Val Ser His Gln Gly
245 250 255
Ser Thr Ala Lys Ile Thr Tyr Arg Asp Pro Ser Thr Asp Thr Leu Thr
260 265 270
Ala Glu Asp Arg Val Asp Thr Ala Ser Val Ala Ala Ala Pro Pro Glu
275 280 285
Val Val
290
<210>319
<211>868
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(838)
<223>RXA02721
<400>319
ccgtgatgat cgtcgtgacg atcgccgcga tgaccgcgga gacgacctgg atgtacccag 60
cttcctccag taattaagaa ggagaataga cttatccact atg gat agt ctt gac 115
Met Asp Ser Leu Asp
1 5
ccc cgc aac cgc ccc gtc cgc aag gtc ttc acg acc cgt gcc ggc ggg 163
Pro Arg Asn Arg Pro Val Arg Lys Val Phe Thr Thr Arg Ala Gly Gly
10 15 20
gtt tcg cag tcc ccg tat gct tcc ttc aac ctt ggt gat cac gtc ggc 211
Val Ser Gln Ser Pro Tyr Ala Ser Phe Asn Leu Gly Asp His Val Gly
25 30 35
gac gat cct caa gct gtg gca tcc aac cgc aat cgc ttg gct gac atc 259
Asp Asp Pro Gln Ala Val Ala Ser Asn Arg Asn Arg Leu Ala Asp Ile
40 45 50
atc ggt ttg tcc cca gac aag gtg gtg tac atg gag caa att cat tcc 307
Ile Gly Leu Ser Pro Asp Lys Val Val Tyr Met Glu Gln Ile His Ser
55 60 65
aat acc gtc aca gtc att gat gaa gcc cca gcg gat ggc cag gct gta 355
Asn Thr Val Thr Val Ile Asp Glu Ala Pro Ala Asp Gly Gln Ala Val
70 75 80 85
gag gcc acc gat gcg cta gtg acc acg caa cga ggg cta gcg ctg gcc 403
Glu Ala Thr Asp Ala Leu Val Thr Thr Gln Arg Gly Leu Ala Leu Ala
90 95 100
gtt ttg gtt gct gat tgc gtg cca gtg ctg ctg tca gac acc gac gct 451
Val Leu Val Ala Asp Cys Val Pro Val Leu Leu Ser Asp Thr Asp Ala
105 110 115
ggc gtg att gcg gca gtg cat gca ggc cgc atg gga gcc cgc aat ggc 499
Gly Val Ile Ala Ala Val His Ala Gly Arg Met Gly Ala Arg Asn Gly
120 125 130
atc gta gct aaa acc att gcg aag atg gag gag ctc ggc gcg aaa ccc 547
Ile Val Ala Lys Thr Ile Ala Lys Met Glu Glu Leu Gly Ala Lys Pro
135 140 145
agc cgc att cat gcg ctc atg ggt gca gcg gca tcg ggt gcg aac tac 595
Ser Arg Ile His Ala Leu Met Gly Ala Ala Ala Ser Gly Ala Asn Tyr
150 155 160 165
gag gtc cca gag gcc atg gcg cgc gat gtg gaa gcc aaa ctt cca ggt 643
Glu Val Pro Glu Ala Met Ala Arg Asp Val Glu Ala Lys Leu Pro Gly
170 175 180
tcc att gcg cgt acg aca aaa ggc acc aca gga ctg gac atc cgc gca 691
Ser Ile Ala Arg Thr Thr Lys Gly Thr Thr Gly Leu Asp Ile Arg Ala
185 190 195
ggg ctg ctg cgt caa atg ctc agc ctg ggt gtg caa atg att gat tct 739
Gly Leu Leu Arg Gln Met Leu Ser Leu Gly Val Gln Met Ile Asp Ser
200 205 210
gat cca cgc tgc acc atc gaa gac gaa gat ctg ttt tct tac cgc cgc 787
Asp Pro Arg Cys Thr Ile Glu Asp Glu Asp Leu Phe Ser Tyr Arg Arg
215 220 225
gaa ggc acg aca ggc cgc cag gcc ggc gtg gtg tgg ctg cca aag gag 835
Glu Gly Thr Thr Gly Arg Gln Ala Gly Val Val Trp Leu Pro Lys Glu
230 235 240 245
gca taaattggag cgccgcgaag agctgcaggt 868
Ala
<210>320
<211>246
<212>PRT
<213>谷氨酸棒状杆菌
<400>320
Met Asp Ser Leu Asp Pro Arg Asn Arg Pro Val Arg Lys Val Phe Thr
1 5 10 15
Thr Arg Ala Gly Gly Val Ser Gln Ser Pro Tyr Ala Ser Phe Asn Leu
20 25 30
Gly Asp His Val Gly Asp Asp Pro Gln Ala Val Ala Ser Asn Arg Asn
35 40 45
Arg Leu Ala Asp Ile Ile Gly Leu Ser Pro Asp Lys Val Val Tyr Met
50 55 60
Glu Gln Ile His Ser Asn Thr Val Thr Val Ile Asp Glu Ala Pro Ala
65 70 75 80
Asp Gly Gln Ala Val Glu Ala Thr Asp Ala Leu Val Thr Thr Gln Arg
85 90 95
Gly Leu Ala Leu Ala Val Leu Val Ala Asp Cys Val Pro Val Leu Leu
100 105 110
Ser Asp Thr Asp Ala Gly Val Ile Ala Ala Val His Ala Gly Arg Met
115 120 125
Gly Ala Arg Asn Gly Ile Val Ala Lys Thr Ile Ala Lys Met Glu Glu
130 135 140
Leu Gly Ala Lys Pro Ser Arg Ile His Ala Leu Met Gly Ala Ala Ala
145 150 155 160
Ser Gly Ala Asn Tyr Glu Val Pro Glu Ala Met Ala Arg Asp Val Glu
165 170 175
Ala Lys Leu Pro Gly Ser Ile Ala Arg Thr Thr Lys Gly Thr Thr Gly
180 185 190
Leu Asp Ile Arg Ala Gly Leu Leu Arg Gln Met Leu Ser Leu Gly Val
195 200 205
Gln Met Ile Asp Ser Asp Pro Arg Cys Thr Ile Glu Asp Glu Asp Leu
210 215 220
Phe Ser Tyr Arg Arg Glu Gly Thr Thr Gly Arg Gln Ala Gly Val Val
225 230 235 240
Trp Leu Pro Lys Glu Ala
245
<210>321
<211>757
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(727)
<223>RXA02725
<400>321
gagttgtttt gttgagaaag cccgctgcac atcggtgtgg cgggttttct catgcccaga 60
tattgacctt gtcactgtgt caaggcattg aataagtgcc atg aac ata agt gcc 115
Met Asn Ile Ser Ala
1 5
atg gac aac ttc gaa cat tca aat gca tct atc cgt tta caa gca gcg 163
Met Asp Asn Phe Glu His Ser Asn Ala Ser Ile Arg Leu Gln Ala Ala
10 15 20
ttg gcc gca gga act gct gag gac acc act gcg atc gat att ctt ttg 211
Leu Ala Ala Gly Thr Ala Glu Asp Thr Thr Ala Ile Asp Ile Leu Leu
25 30 35
tac cgc agt ggg gta gaa gaa gac ttc ttt gtg cgc gac atg atc acg 259
Tyr Arg Ser Gly Val Glu Glu Asp Phe Phe Val Arg Asp Met Ile Thr
40 45 50
tgg gcg ctc acc cgc atg cca gtt gat gac gtg ttt acc tca ctg gtc 307
Trp Ala Leu Thr Arg Met Pro Val Asp Asp Val Phe Thr Ser Leu Val
55 60 65
gct gat ttg gat tct gag gta cct gat cga cca ttc ggt gca agt caa 355
Ala Asp Leu Asp Ser Glu Val Pro Asp Arg Pro Phe Gly Ala Ser Gln
70 75 80 85
gca ctc cac aca ctc tcc aag tta agg gtc gcg cag tcg tgg cag gaa 403
Ala Leu His Thr Leu Ser Lys Leu Arg Val Ala Gln Ser Trp Gln Glu
90 95 100
ttg caa gca cgg cct tgg ctt ttg cac cgg gag gac acc gcc caa act 451
Leu Gln Ala Arg Pro Trp Leu Leu His Arg Glu Asp Thr Ala Gln Thr
105 110 115
gcg tgg cga acc ttt gtg gga ctg gtc cca gat gat cag acc gca tgg 499
Ala Trp Arg Thr Phe Val Gly Leu Val Pro Asp Asp Gln Thr Ala Trp
120 125 130
ttg gcg cac caa ttg ctc caa gaa ttg gat aaa gga acg cct gaa att 547
Leu Ala His Gln Leu Leu Gln Glu Leu Asp Lys Gly Thr Pro Glu Ile
135 140 145
cag cgg agt tta agt cgg gcg atg gcg gaa ttg gag ggg agg gag gcg 595
Gln Arg Ser Leu Ser Arg Ala Met Ala Glu Leu Glu Gly Arg Glu Ala
150 155 160 165
tcg ata agc gtg ctt cta ggc gct tta acg agc gcg cac gcg gtg gcc 643
Ser Ile Ser Val Leu Leu Gly Ala Leu Thr Ser Ala His Ala Val Ala
170 175 180
acc gcc aag ctc att gcg gat ccg gac tcc gat ttc atg gct gat ctg 691
Thr Ala Lys Leu Ile Ala Asp Pro Asp Ser Asp Phe Met Ala Asp Leu
185 190 195
gag gaa gcg cgg cgc gtg gat aat atg ggt gca tgc tgatcggtga 737
Glu Glu Ala Arg Arg Val Asp Asn Met Gly Ala Cys
200 205
ggtgtccaag ctcagtgggg 757
<210>322
<211>209
<212>PRT
<213>谷氨酸棒状杆菌
<400>322
Met Asn Ile Ser Ala Met Asp Asn Phe Glu His Ser Asn Ala Ser Ile
1 5 10 15
Arg Leu Gln Ala Ala Leu Ala Ala Gly Thr Ala Glu Asp Thr Thr Ala
20 25 30
Ile Asp Ile Leu Leu Tyr Arg Ser Gly Val Glu Glu Asp Phe Phe Val
35 40 45
Arg Asp Met Ile Thr Trp Ala Leu Thr Arg Met Pro Val Asp Asp Val
50 55 60
Phe Thr Ser Leu Val Ala Asp Leu Asp Ser Glu Val Pro Asp Arg Pro
65 70 75 80
Phe Gly Ala Ser Gln Ala Leu His Thr Leu Ser Lys Leu Arg Val Ala
85 90 95
Gln Ser Trp Gln Glu Leu Gln Ala Arg Pro Trp Leu Leu His Arg Glu
100 105 110
Asp Thr Ala Gln Thr Ala Trp Arg Thr Phe Val Gly Leu Val Pro Asp
115 120 125
Asp Gln Thr Ala Trp Leu Ala His Gln Leu Leu Gln Glu Leu Asp Lys
130 135 140
Gly Thr Pro Glu Ile Gln Arg Ser Leu Ser Arg Ala Met Ala Glu Leu
145 150 155 160
Glu Gly Arg Glu Ala Ser Ile Ser Val Leu Leu Gly Ala Leu Thr Ser
165 170 175
Ala His Ala Val Ala Thr Ala Lys Leu Ile Ala Asp Pro Asp Ser Asp
180 185 190
Phe Met Ala Asp Leu Glu Glu Ala Arg Arg Val Asp Asn Met Gly Ala
195 200 205
Cys
<210>323
<211>955
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(925)
<223>RXA02735
<400>323
accggctcga tcatcatcac catctatgac gctcataccc ttcaggtaga gatgccggaa 60
tccggcaatg ccccatcgct ggtggctatt ggtcgtcgaa gtg agt ccg act gct 115
Val Ser Pro Thr Ala
1 5
tgt ctg agg agc ttc gcc aca tgg atc cag att tgg gct acc agc acg 163
Cys Leu Arg Ser Phe Ala Thr Trp Ile Gln Ile Trp Ala Thr Ser Thr
10 15 20
cac tat ccg gct tgt cca gcg tca agc tgg aaa ccg tct aag gag aaa 211
His Tyr Pro Ala Cys Pro Ala Ser Ser Trp Lys Pro Ser Lys Glu Lys
25 30 35
tac aac act atg gtt gat gta gta cgc gca cgc gat act gaa gat ttg 259
Tyr Asn Thr Met Val Asp Val Val Arg Ala Arg Asp Thr Glu Asp Leu
40 45 50
gtt gca cag gct gcc tcc aaa ttc att gag gtt gtt gaa gca gca act 307
Val Ala Gln Ala Ala Ser Lys Phe Ile Glu Val Val Glu Ala Ala Thr
55 60 65
gcc aat aat ggc acc gca cag gta gtg ctc acc ggt ggt ggc gcc ggc 355
Ala Asn Asn Gly Thr Ala Gln Val Val Leu Thr Gly Gly Gly Ala Gly
70 75 80 85
atc aag ttg ctg gaa aag ctc agc gtt gat gcg gct gac ctt gcc tgg 403
Ile Lys Leu Leu Glu Lys Leu Ser Val Asp Ala Ala Asp Leu Ala Trp
90 95 100
gat cgc att cat gtg ttc ttc ggc gat gag cgc aat gtc cct gtc agt 451
Asp Arg Ile His Val Phe Phe Gly Asp Glu Arg Asn Val Pro Val Ser
105 110 115
gat tct gag tcc aat gag ggc cag gct cgt gag gca ctg ttg tcc aag 499
Asp Ser Glu Ser Asn Glu Gly Gln Ala Arg Glu Ala Leu Leu Ser Lys
120 125 130
gtt tct atc cct gaa gcc aac att cac gga tat ggt ctc ggc gac gta 547
Val Ser Ile Pro Glu Ala Asn Ile His Gly Tyr Gly Leu Gly Asp Val
135 140 145
gat ctt gca gag gca gcc cgc gct tac gaa gct gtg ttg gat gaa ttc 595
Asp Leu Ala Glu Ala Ala Arg Ala Tyr Glu Ala Val Leu Asp Glu Phe
150 155 160 165
gca cca aac ggc ttt gat ctt cac ctg ctc ggc atg ggt ggc gaa ggc 643
Ala Pro Asn Gly Phe Asp Leu His Leu Leu Gly Met Gly Gly Glu Gly
170 175 180
cat atc aac tcc ctg ttc cct cac acc gat gca gtc aag gaa tcc tcc 691
His Ile Asn Ser Leu Phe Pro His Thr Asp Ala Val Lys Glu Ser Ser
185 190 195
gca aag gtc atc gcg gtg ttt gat tcc cct aag cct cct tca gag cgt 739
Ala Lys Val Ile Ala Val Phe Asp Ser Pro Lys Pro Pro Ser Glu Arg
200 205 210
gca act cta acc ctt cct gcg gtt cac tcc gca aag cgc gtg tgg ttg 787
Ala Thr Leu Thr Leu Pro Ala Val His Ser Ala Lys Arg Val Trp Leu
215 220 225
ctg gtt tct ggt gcg gag aag gct gag gca gct gcg gcg atc gtc aac 835
Leu Val Ser Gly Ala Glu Lys Ala Glu Ala Ala Ala Ala Ile Val Asn
230 235 240 245
ggt gag cct gct gtt gag tgg cct gct gct gga gct acc gga tct gag 883
Gly Glu Pro Ala Val Glu Trp Pro Ala Ala Gly Ala Thr Gly Ser Glu
250 255 260
gaa acg gta ttg ttc ttg gct gat gat gct gca gga aat ctc 925
Glu Thr Val Leu Phe Leu Ala Asp Asp Ala Ala Gly Asn Leu
265 270 275
taagcagcgc cagctctaac aagaagcttt 955
<210>324
<211>275
<212>PRT
<213>谷氨酸棒状杆菌
<400>324
Val Ser Pro Thr Ala Cys Leu Arg Ser Phe Ala Thr Trp Ile Gln Ile
1 5 10 15
Trp Ala Thr Ser Thr His Tyr Pro Ala Cys Pro Ala Ser Ser Trp Lys
20 25 30
Pro Ser Lys Glu Lys Tyr Asn Thr Met Val Asp Val Val Arg Ala Arg
35 40 45
Asp Thr Glu Asp Leu Val Ala Gln Ala Ala Ser Lys Phe Ile Glu Val
50 55 60
Val Glu Ala Ala Thr Ala Asn Asn Gly Thr Ala Gln Val Val Leu Thr
65 70 75 80
Gly Gly Gly Ala Gly Ile Lys Leu Leu Glu Lys Leu Ser Val Asp Ala
85 90 95
Ala Asp Leu Ala Trp Asp Arg Ile His Val Phe Phe Gly Asp Glu Arg
100 105 110
Asn Val Pro Val Ser Asp Ser Glu Ser Asn Glu Gly Gln Ala Arg Glu
115 120 125
Ala Leu Leu Ser Lys Val Ser Ile Pro Glu Ala Asn Ile His Gly Tyr
130 135 140
Gly Leu Gly Asp Val Asp Leu Ala Glu Ala Ala Arg Ala Tyr Glu Ala
145 150 155 160
Val Leu Asp Glu Phe Ala Pro Asn Gly Phe Asp Leu His Leu Leu Gly
165 170 175
Met Gly Gly Glu Gly His Ile Asn Ser Leu Phe Pro His Thr Asp Ala
180 185 190
Val Lys Glu Ser Ser Ala Lys Val Ile Ala Val Phe Asp Ser Pro Lys
195 200 205
Pro Pro Ser Glu Arg Ala Thr Leu Thr Leu Pro Ala Val His Ser Ala
210 215 220
Lys Arg Val Trp Leu Leu Val Ser Gly Ala Glu Lys Ala Glu Ala Ala
225 230 235 240
Ala Ala Ile Val Asn Gly Glu Pro Ala Val Glu Trp Pro Ala Ala Gly
245 250 255
Ala Thr Gly Ser Glu Glu Thr Val Leu Phe Leu Ala Asp Asp Ala Ala
260 265 270
Gly Asn Leu
275
<210>325
<211>1087
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1057)
<223>RXA02736
<400>325
cagaggatta cccagcgggt acgtggggtc caaagagcgc tgatgaaatg ctttcccgca 60
acggtcacac ctggcgcagg ccataattta ggggcaaaaa atg atc ttt gaa ctt 115
Met Ile Phe Glu Leu
1 5
ccg gat acc acc acc cag caa att tcc aag acc cta act cga ctg cgt 163
Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr Leu Thr Arg Leu Arg
10 15 20
gaa tcg ggc acc cag gtc acc acc ggc cga gtg ctc acc ctc atc gtg 211
Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val Leu Thr Leu Ile Val
25 30 35
gtc act gac tcc gaa agc gat gtc gct gca gtt acc gag tcc acc aat 259
Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val Thr Glu Ser Thr Asn
40 45 50
gaa gcc tcg cgc gag cac cca tct cgc gtg atc att ttg gtg gtt ggc 307
Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile Ile Leu Val Val Gly
55 60 65
gat aaa act gca gaa aac aaa gtt gac gca gaa gtc cgt atc ggt ggc 355
Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu Val Arg Ile Gly Gly
70 75 80 85
gac gct ggt gct tcc gag atg atc atc atg cat ctc aac gga cct gtc 403
Asp Ala Gly Ala Ser Glu Met Ile Ile Met His Leu Asn Gly Pro Val
90 95 100
gct gac aag ctc cag tat gtc gtc aca cca ctg ttg ctt cct gac acc 451
Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu Leu Leu Pro Asp Thr
105 110 115
ccc atc gtt gct tgg tgg cca ggt gaa tca cca aag aat cct tcc cag 499
Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro Lys Asn Pro Ser Gln
120 125 130
gac cca att gga cgc atc gca caa cga cgc atc act gat gct ttg tac 547
Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile Thr Asp Ala Leu Tyr
135 140 145
gac cgt gat gac gca cta gaa gat cgt gtt gag aac tat cac cca ggt 595
Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu Asn Tyr His Pro Gly
150 155 160 165
gat acc gac atg acg tgg gcg cgc ctt acc cag tgg cgg gga ctt gtt 643
Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln Trp Arg Gly Leu Val
170 175 180
gcc tcc tca ttg gat cac cca cca cac agc gaa atc act tcc gtg agg 691
Ala Ser Ser Leu Asp His Pro Pro His Ser Glu Ile Thr Ser Val Arg
185 190 195
ctg acc ggt gca agc ggc agt acc tcg gtg gat ttg gct gca ggc tgg 739
Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp Leu Ala Ala Gly Trp
200 205 210
ttg gcg cgg agg ctg aaa gtg cct gtg atc cgc gag gtg aca gat gct 787
Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg Glu Val Thr Asp Ala
215 220 225
ccc acc gtg cca acc gat gag ttt ggt act cca ctg ctg gct atc cag 835
Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro Leu Leu Ala Ile Gln
230 235 240 245
cgc ctg gag atc gtt cgc acc acc ggc tcg atc atc atc acc atc tat 883
Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile Ile Ile Thr Ile Tyr
250 255 260
gac gct cat acc ctt cag gta gag atg ccg gaa tcc ggc aat gcc cca 931
Asp Ala His Thr Leu Gln Val Glu Met Pro Glu Ser Gly Asn Ala Pro
265 270 275
tcg ctg gtg gct att ggt cgt cga agt gag tcc gac tgc ttg tct gag 979
Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser Asp Cys Leu Ser Glu
280 285 290
gag ctt cgc cac atg gat cca gat ttg ggc tac cag cac gca cta tcc 1027
Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr Gln His Ala Leu Ser
295 300 305
ggc ttg tcc agc gtc aag ctg gaa acc gtc taaggagaaa tacaacacta 1077
Gly Leu Ser Ser Val Lys Leu Glu Thr Val
310 315
tggttgatgt 1087
<210>326
<211>319
<212>PRT
<213>谷氨酸棒状杆菌
<400>326
Met Ile Phe Glu Leu Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr
1 5 10 15
Leu Thr Arg Leu Arg Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val
20 25 30
Leu Thr Leu Ile Val Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val
35 40 45
Thr Glu Ser Thr Asn Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile
50 55 60
Ile Leu Val Val Gly Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu
65 70 75 80
Val Arg Ile Gly Gly Asp Ala Gly Ala Ser Glu Met Ile Ile Met His
85 90 95
Leu Asn Gly Pro Val Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu
100 105 110
Leu Leu Pro Asp Thr Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro
115 120 125
Lys Asn Pro Ser Gln Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile
130 135 140
Thr Asp Ala Leu Tyr Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu
145 150 155 160
Asn Tyr His Pro Gly Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln
165 170 175
Trp Arg Gly Leu Val Ala Ser Ser Leu Asp His Pro Pro His Ser Glu
180 185 190
Ile Thr Ser Val Arg Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp
195 200 205
Leu Ala Ala Gly Trp Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg
210 215 220
Glu Val Thr Asp Ala Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro
225 230 235 240
Leu Leu Ala Ile Gln Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile
245 250 255
Ile Ile Thr Ile Tyr Asp Ala His Thr Leu Gln Val Glu Met Pro Glu
260 265 270
Ser Gly Asn Ala Pro Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser
275 280 285
Asp Cys Leu Ser Glu Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr
290 295 300
Gln His Ala Leu Ser Gly Leu Ser Ser Val Lys Leu Glu Thr Val
305 310 315
<210>327
<21l>934
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(904)
<223>RXA02744
<400>327
gccaaaacgt catgattcgc tctttggata cgggacaccg ctcattggaa gatgtcttcc 60
tggacatcac cggaaaagaa ctgaggagtt aacgcacacc atg tct aaa cct ttt 115
Met Ser Lys Pro Phe
1 5
gaa aac tct gcg ctc cgc ggt tct tct cga ttc cca gct gga acg ttc 163
Glu Asn Ser Ala Leu Arg Gly Ser Ser Arg Phe Pro Ala Gly Thr Phe
10 15 20
acc cct gct ccc aaa cga gcc acc ccg gca aaa atg ttg gct gct cag 211
Thr Pro Ala Pro Lys Arg Ala Thr Pro Ala Lys Met Leu Ala Ala Gln
25 30 35
ggc aag atg gaa tcc ctg ctg ttt ctt cgc cac ggc gaa cag caa ctg 259
Gly Lys Met Glu Ser Leu Leu Phe Leu Arg His Gly Glu Gln Gln Leu
40 45 50
ctc agc atc atc att ccc ttg gtc gcg ctc atc gca cta gcg aat ttt 307
Leu Ser Ile Ile Ile Pro Leu Val Ala Leu Ile Ala Leu Ala Asn Phe
55 60 65
gat ttc atc cct ggt gag aac tcc ctc gac aag act ttc ccc ttc gcg 355
Asp Phe Ile Pro Gly Glu Asn Ser Leu Asp Lys Thr Phe Pro Phe Ala
70 75 80 85
ctg gcc aca gca gcc atg agc gct ggt ttt aca ggt caa gcc atc agc 403
Leu Ala Thr Ala Ala Met Ser Ala Gly Phe Thr Gly Gln Ala Ile Ser
90 95 100
cta gct ttt gac cgc cgc tat ggt gcc ctc aag cgc acc ggc gcc agc 451
Leu Ala Phe Asp Arg Arg Tyr Gly Ala Leu Lys Arg Thr Gly Ala Ser
105 110 115
ggt gtt ccc gcc tgg acg att att ttt ggc aaa gtc atc gca gtc att 499
Gly Val Pro Ala Trp Thr Ile Ile Phe Gly Lys Val Ile Ala Val Ile
120 125 130
gca gtc acc att gtg cag atc atc ttt ctc ggt gtg act gca ctg ctg 547
Ala Val Thr Ile Val Gln Ile Ile Phe Leu Gly Val Thr Ala Leu Leu
135 140 145
ttg ggc tgg tcc gca cct gtc ggt ggt gtg ctc ttt ggc atc gtg acc 595
Leu Gly Trp Ser Ala Pro Val Gly Gly Val Leu Phe Gly Ile Val Thr
150 155 160 165
cta ttt gtg ggt gtt tcc agc ttc acc gcg ctc ggc atg ctg atg ggc 643
Leu Phe Val Gly Val Ser Ser Phe Thr Ala Leu Gly Met Leu Met Gly
170 175 180
gga acg ttg tcc tcc gaa ttg gta ttg gca ctg gct aac ttg att tgg 691
Gly Thr Leu Ser Ser Glu Leu Val Leu Ala Leu Ala Asn Leu Ile Trp
185 190 195
att gta ctg tcc ggc ctt gca gca tgg gcg gtc ttt tcc cct tcc gtc 739
Ile Val Leu Ser Gly Leu Ala Ala Trp Ala Val Phe Ser Pro Ser Val
200 205 210
aac gct gaa gga gtg ctg tcc atc atc cca tcc gtt gcg ctg tcc caa 787
Asn Ala Glu Gly Val Leu Ser Ile Ile Pro Ser Val Ala Leu Ser Gln
215 220 225
ggt atg gtt gac gca ttc aac ggc gaa ctt ccg tgg ctc cag cta gga 835
Gly Met Val Asp Ala Phe Asn Gly Glu Leu Pro Trp Leu Gln Leu Gly
230 235 240 245
att ttg gtg ggc tgg cta att atc acc ggc gtg gcc gca aac aag cta 883
Ile Leu Val Gly Trp Leu Ile Ile Thr Gly Val Ala Ala Asn Lys Leu
250 255 260
ttt aac ttc tct gcg agc cgc tagatatacc cttagtcgga aaatatcctt 934
Phe Asn Phe Ser Ala Ser Arg
265
<210>328
<211>268
<212>PRT
<213>谷氨酸棒状杆菌
<400>328
Met Ser Lys Pro Phe Glu Asn Ser Ala Leu Arg Gly Ser Ser Arg Phe
1 5 10 15
Pro Ala Gly Thr Phe Thr Pro Ala Pro Lys Arg Ala Thr Pro Ala Lys
20 25 30
Met Leu Ala Ala Gln Gly Lys Met Glu Ser Leu Leu Phe Leu Arg His
35 40 45
Gly Glu Gln Gln Leu Leu Ser Ile Ile Ile Pro Leu Val Ala Leu Ile
50 55 60
Ala Leu Ala Asn Phe Asp Phe Ile Pro Gly Glu Asn Ser Leu Asp Lys
65 70 75 80
Thr Phe Pro Phe Ala Leu Ala Thr Ala Ala Met Ser Ala Gly Phe Thr
85 90 95
Gly Gln Ala Ile Ser Leu Ala Phe Asp Arg Arg Tyr Gly Ala Leu Lys
100 105 110
Arg Thr Gly Ala Ser Gly Val Pro Ala Trp Thr Ile Ile Phe Gly Lys
115 120 125
Val Ile Ala Val Ile Ala Val Thr Ile Val Gln Ile Ile Phe Leu Gly
130 135 140
Val Thr Ala Leu Leu Leu Gly Trp Ser Ala Pro Val Gly Gly Val Leu
145 150 155 160
Phe Gly Ile Val Thr Leu Phe Val Gly Val Ser Ser Phe Thr Ala Leu
165 170 175
Gly Met Leu Met Gly Gly Thr Leu Ser Ser Glu Leu Val Leu Ala Leu
180 185 190
Ala Asn Leu Ile Trp Ile Val Leu Ser Gly Leu Ala Ala Trp Ala Val
195 200 205
Phe Ser Pro Ser Val Asn Ala Glu Gly Val Leu Ser Ile Ile Pro Ser
210 215 220
Val Ala Leu Ser Gln Gly Met Val Asp Ala Phe Asn Gly Glu Leu Pro
225 230 235 240
Trp Leu Gln Leu Gly Ile Leu Val Gly Trp Leu Ile Ile Thr Gly Val
245 250 255
Ala Ala Asn Lys Leu Phe Asn Phe Ser Ala Ser Arg
260 265
<210>329
<211>853
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(823)
<223>RXA02757
<400>329
ccattcctcg acgaggtttt gcacatcatg ggcatttccc gcgacgagat cgatctggcg 60
gatcaggaag acggcacttt ccaccgcgtt ccattgacca atg cct aaa gat tcg 115
Met Pro Lys Asp Ser
1 5
ctt ctc gac gcc cac ctc ctc ctc aag gcc cgg gct agc gac ggg cca 163
Leu Leu Asp Ala His Leu Leu Leu Lys Ala Arg Ala Ser Asp Gly Pro
10 15 20
cat agc gag gat cct tcg gat cct tcg acc gtt cag gca atg cag atc 211
His Ser Glu Asp Pro Ser Asp Pro Ser Thr Val Gln Ala Met Gln Ile
25 30 35
gcg ttg cac att ccg aaa cag aat ccg ccc cgg cgg aca gat gtg ttg 259
Ala Leu His Ile Pro Lys Gln Asn Pro Pro Arg Arg Thr Asp Val Leu
40 45 50
gaa gcg gcg gcg agg agt gtg gtc aag ctt tgc ctc gac gaa cga gta 307
Glu Ala Ala Ala Arg Ser Val Val Lys Leu Cys Leu Asp Glu Arg Val
55 60 65
tcc acc gat cct gat ttt cgg gcg gcc ttg gaa cgt tgg tac gga cac 355
Ser Thr Asp Pro Asp Phe Arg Ala Ala Leu Glu Arg Trp Tyr Gly His
70 75 80 85
ttg att cgg aag gtg tca cgt cgc gct cgt aat gcg gcg tgg gat cgg 403
Leu Ile Arg Lys Val Ser Arg Arg Ala Arg Asn Ala Ala Trp Asp Arg
90 95 100
gtg caa gat tta ccc ggc gtg act gtg gaa gat gat gct gca aag gtt 451
Val Gln Asp Leu Pro Gly Val Thr Val Glu Asp Asp Ala Ala Lys Val
105 110 115
cgg gca ttt ttg ccc agc gca gtg gtg gat gtg cct gca gat atc aaa 499
Arg Ala Phe Leu Pro Ser Ala Val Val Asp Val Pro Ala Asp Ile Lys
120 125 130
aag ctg cag att tcc ggt acg gaa ttg cct ctc gat gag ccg aat ccg 547
Lys Leu Gln Ile Ser Gly Thr Glu Leu Pro Leu Asp Glu Pro Asn Pro
135 140 145
atc aac gat gaa tac ccc gtt atc tac att gat gaa tcg ttg aag atg 595
Ile Asn Asp Glu Tyr Pro Val Ile Tyr Ile Asp Glu Ser Leu Lys Met
150 155 160 165
acg ctg gga aag gcc gct gcg cag gta gga cac gca tcc atg ttg ctt 643
Thr Leu Gly Lys Ala Ala Ala Gln Val Gly His Ala Ser Met Leu Leu
170 175 180
gcc gcg cac caa ccg ttt gag tgg gtg gag cag tgg gaa gca gcg gat 691
Ala Ala His Gln Pro Phe Glu Trp Val Glu Gln Trp Glu Ala Ala Asp
185 190 195
ttt gcg ctg cac gtc agg gag att ccc tcg gag gag ttc ctg cgc ctt 739
Phe Ala Leu His Val Arg Glu Ile Pro Ser Glu Glu Phe Leu Arg Leu
200 205 210
atc gag tct cct ggt gct gtt cca gtt cgc gat ggc ggc ttt act gag 787
Ile Glu Ser Pro Gly Ala Val Pro Val Arg Asp Gly Gly Phe Thr Glu
215 220 225
gtt gcc ccc aac acc gtg acg gtt gtt gca att cca taactaaacg 833
Val Ala Pro Asn Thr Val Thr Val Val Ala Ile Pro
230 235 240
agctttgatt aacaagtcct 853
<210>330
<211>241
<212>PRT
<213>谷氨酸棒状杆菌
<400>330
Met Pro Lys Asp Ser Leu Leu Asp Ala His Leu Leu Leu Lys Ala Arg
1 5 10 15
Ala Ser Asp Gly Pro His Ser Glu Asp Pro Ser Asp Pro Ser Thr Val
20 25 30
Gln Ala Met Gln Ile Ala Leu His Ile Pro Lys Gln Asn Pro Pro Arg
35 40 45
Arg Thr Asp Val Leu Glu Ala Ala Ala Arg Ser Val Val Lys Leu Cys
50 55 60
Leu Asp Glu Arg Val Ser Thr Asp Pro Asp Phe Arg Ala Ala Leu Glu
65 70 75 80
Arg Trp Tyr Gly His Leu Ile Arg Lys Val Ser Arg Arg Ala Arg Asn
85 90 95
Ala Ala Trp Asp Arg Val Gln Asp Leu Pro Gly Val Thr Val Glu Asp
100 105 110
Asp Ala Ala Lys Val Arg Ala Phe Leu Pro Ser Ala Val Val Asp Val
115 120 125
Pro Ala Asp Ile Lys Lys Leu Gln Ile Ser Gly Thr Glu Leu Pro Leu
130 135 140
Asp Glu Pro Asn Pro Ile Asn Asp Glu Tyr Pro Val Ile Tyr Ile Asp
145 150 155 160
Glu Ser Leu Lys Met Thr Leu Gly Lys Ala Ala Ala Gln Val Gly His
165 170 175
Ala Ser Met Leu Leu Ala Ala His Gln Pro Phe Glu Trp Val Glu Gln
180 185 190
Trp Glu Ala Ala Asp Phe Ala Leu His Val Arg Glu Ile Pro Ser Glu
195 200 205
Glu Phe Leu Arg Leu Ile Glu Ser Pro Gly Ala Val Pro Val Arg Asp
210 215 220
Gly Gly Phe Thr Glu Val Ala Pro Asn Thr Val Thr Val Val Ala Ile
225 230 235 240
Pro
<210>331
<211>1237
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1207)
<223>RXA02777
<400>331
ggtcgcacca caccaactac tgtttcttct gtaatttgca ctgaaactat cgcctacact 60
gtcactgcta ccatcacaat cggttgctag taggctacaa atg aac cgc ccc ctt 115
Met Asn Arg Pro Leu
1 5
caa agt caa aat ttt tca ata acc agc gaa tgg gca aat gcc acg ttc 163
Gln Ser Gln Asn Phe Ser Ile Thr Ser Glu Trp Ala Asn Ala Thr Phe
10 15 20
aac gat atc gaa gac tct ttg gag ggg tct ttt gac atc gaa cca ggc 211
Asn Asp Ile Glu Asp Ser Leu Glu Gly Ser Phe Asp Ile Glu Pro Gly
25 30 35
caa tac att gga cgc gga gaa tac ttc gta gct act tct gga acg ggt 259
Gln Tyr Ile Gly Arg Gly Glu Tyr Phe Val Ala Thr Ser Gly Thr Gly
40 45 50
tcc aaa aag ttt atc aag gtg gtt ttg ggt gac ggt atc gat agc gaa 307
Ser Lys Lys Phe Ile Lys Val Val Leu Gly Asp Gly Ile Asp Ser Glu
55 60 65
cta aat cgt cgc atc gaa ggt gaa att ctt gca agt agt ctc cat ctt 355
Leu Asn Arg Arg Ile Glu Gly Glu Ile Leu Ala Ser Ser Leu His Leu
70 75 80 85
cca tcc aca gac cta atc tct cct gtc gta att cag tat gtg aaa gag 403
Pro Ser Thr Asp Leu Ile Ser Pro Val Val Ile Gln Tyr Val Lys Glu
90 95 100
tct tct aat ttt ccc ggt gac ttc tac atc gtg tcc gac ttc aaa gac 451
Ser Ser Asn Phe Pro Gly Asp Phe Tyr Ile Val Ser Asp Phe Lys Asp
105 110 115
gac aca gaa act tta gag cta tct aag gct tct cta ccc ctc act gac 499
Asp Thr Glu Thr Leu Glu Leu Ser Lys Ala Ser Let Pro Leu Thr Asp
120 125 130
tta gcc aag atc gga gcg ttt ctc ttt cgt ttg gaa tct gta aca gtt 547
Leu Ala Lys Ile Gly Ala Phe Leu Phe Arg Leu Glu Ser Val Thr Val
135 140 145
ccc cct caa aat cct gca agg cga gca atg gat gtt ttg gat gga ctt 595
Pro Pro Gln Asn Pro Ala Arg Arg Ala Met Asp Val Leu Asp Gly Leu
150 155 160 165
tct ctt ctt gca aat aaa ggc aac att gaa aca ggc gca caa tta aca 643
Ser Leu Leu Ala Asn Lys Gly Asn Ile Glu Thr Gly Ala Gln Leu Thr
170 175 180
aaa gga act ata caa gct ctt gaa atc att aat caa gaa gcg acc aac 691
Lys Gly Thr Ile Gln Ala Leu Glu Ile Ile Asn Gln Glu Ala Thr Asn
185 190 195
atc gag aat gca ctt gaa aaa gta act aaa gta gga tca cgg aag ttt 739
Ile Glu Asn Ala Leu Glu Lys Val Thr Lys Val Gly Ser Arg Lys Phe
200 205 210
ttc tcg cat gga gat ctt aaa ttt tct cag ttc tta att aat ggt gaa 787
Phe Ser His Gly Asp Leu Lys Phe Ser Gln Phe Leu Ile Asn Gly Glu
215 220 225
acc gga aag att ttc cta tgt gac tgg gaa gaa tgc ggt agt gca cat 835
Thr Gly Lys Ile Phe Leu Cys Asp Trp Glu Glu Cys Gly Ser Ala His
230 235 240 245
ttc gca aat gac cta tgt ttt ctc gca ggt gat ttg ttc tat agc aca 883
Phe Ala Asn Asp Leu Cys Phe Leu Ala Gly Asp Leu Phe Tyr Ser Thr
250 255 260
att cgg gaa ctg gtg gat acc aac atc aaa aac att aaa ggc tcc tcg 931
Ile Arg Glu Leu Val Asp Thr Asn Ile Lys Asn Ile Lys Gly Ser Ser
265 270 275
atc att cag gaa gcc tac gat aat gca acg ctt gag gct gta tca aaa 979
Ile Ile Gln Glu Ala Tyr Asp Asn Ala Thr Leu Glu Ala Val Ser Lys
280 285 290
gta aat gca ata tta act ggc tat tcc tca gag agg ggt tac tct ctc 1027
Val Asn Ala Ile Leu Thr Gly Tyr Ser Ser Glu Arg Gly Tyr Ser Leu
295 300 305
acc tcg gac gaa aaa cga atc att agt atc aga atc gga ttg gct ggt 1075
Thr Ser Asp Glu Lys Arg Ile Ile Ser Ile Arg Ile Gly Leu Ala Gly
310 315 320 325
cta ttt cga ctt tac act gtg agc gct aaa tct aat gag tta cgc ccg 1123
Leu Phe Arg Leu Tyr Thr Val Ser Ala Lys Ser Asn Glu Leu Arg Pro
330 335 340
cgc gaa tta gcc tta gcc tcc atc gga atg caa att act tta ggt caa 1171
Arg Glu Leu Ala Leu Ala Ser Ile Gly Met Gln Ile Thr Leu Gly Gln
345 350 355
gca ccc aat ctg gtg ttt aca gat agc aag gtg gtt taagtgagga 1217
Ala Pro Asn Leu Val Phe Thr Asp Ser Lys Val Val
360 365
atttgcacga aacgatagac 1237
<210>332
<211>369
<212>PRT
<213>谷氨酸棒状杆菌
<400>332
Met Asn Arg Pro Leu Gln Ser Gln Asn Phe Ser Ile Thr Ser Glu Trp
1 5 10 15
Ala Asn Ala Thr Phe Asn Asp Ile Glu Asp Ser Leu Glu Gly Ser Phe
20 25 30
Asp Ile Glu Pro Gly Gln Tyr Ile Gly Arg Gly Glu Tyr Phe Val Ala
35 40 45
Thr Ser Gly Thr Gly Ser Lys Lys Phe Ile Lys Val Val Leu Gly Asp
50 55 60
Gly Ile Asp Ser Glu Leu Asn Arg Arg Ile Glu Gly Glu Ile Leu Ala
65 70 75 80
Ser Ser Leu His Leu Pro Ser Thr Asp Leu Ile Ser Pro Val Val Ile
85 90 95
Gln Tyr Val Lys Glu Ser Ser Asn Phe Pro Gly Asp Phe Tyr Ile Val
100 105 110
Ser Asp Phe Lys Asp Asp Thr Glu Thr Leu Glu Leu Ser Lys Ala Ser
115 120 125
Leu Pro Leu Thr Asp Leu Ala Lys Ile Gly Ala Phe Leu Phe Arg Leu
130 135 140
Glu Ser Val Thr Val Pro Pro Gln Asn Pro Ala Arg Arg Ala Met Asp
145 150 155 160
Val Leu Asp Gly Leu Ser Leu Leu Ala Asn Lys Gly Asn Ile Glu Thr
165 170 175
Gly Ala Gln Leu Thr Lys Gly Thr Ile Gln Ala Leu Glu Ile Ile Asn
180 185 190
Gln Glu Ala Thr Asn Ile Glu Asn Ala Leu Glu Lys Val Thr Lys Val
195 200 205
Gly Ser Arg Lys Phe Phe Ser His Gly Asp Leu Lys Phe Ser Gln Phe
210 215 220
Leu Ile Asn Gly Glu Thr Gly Lys Ile Phe Leu Cys Asp Trp Glu Glu
225 230 235 240
Cys Gly Ser Ala His Phe Ala Asn Asp Leu Cys Phe Leu Ala Gly Asp
245 250 255
Leu Phe Tyr Ser Thr Ile Arg Glu Leu Val Asp Thr Asn Ile Lys Asn
260 265 270
Ile Lys Gly Ser Ser Ile Ile Gln Glu Ala Tyr Asp Asn Ala Thr Leu
275 280 285
Glu Ala Val Ser Lys Val Asn Ala Ile Leu Thr Gly Tyr Ser Ser Glu
290 295 300
Arg Gly Tyr Ser Leu Thr Ser Asp Glu Lys Arg Ile Ile Ser Ile Arg
305 310 315 320
Ile Gly Leu Ala Gly Leu Phe Arg Leu Tyr Thr Val Ser Ala Lys Ser
325 330 335
Asn Glu Leu Arg Pro Arg Glu Leu Ala Leu Ala Ser Ile Gly Met Gln
340 345 350
Ile Thr Leu Gly Gln Ala Pro Asn Leu Val Phe Thr Asp Ser Lys Val
355 360 365
Val
<210>333
<211>316
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(286)
<223>RXA02784
<400>333
agctgcaact caggtagaag aaacaacagt cgcccgagtt gaacacgata cttacgacac 60
tattgctaac cagctacaag atgccggtgt ctatgacgat gtg gcc ttt gcc gaa 115
Val Ala Phe Ala Glu
1 5
ggc tac tac cag gaa atg gct gag gtt gta tgt gaa gca cgc gaa gag 163
Gly Tyr Tyr Gln Glu Met Ala Glu Val Val Cys Glu Ala Arg Glu Glu
10 15 20
ggt atc acc cac gag gac acc ctg cat atg ctc aat gac atg atg gca 211
Gly Ile Thr His Glu Asp Thr Leu His Met Leu Asn Asp Met Met Ala
25 30 35
atg aaa cga tct ggc gaa gta ctc aat acg ctc gct gat gtt ctc tat 259
Met Lys Arg Ser Gly Glu Val Leu Asn Thr Leu Ala Asp Val Leu Tyr
40 45 50
gca aat gaa tgc cct gaa tat cga atc taaattgttg gttcactctg 306
Ala Asn Glu Cys Pro Glu Tyr Arg Ile
55 60
catagacaca 316
<2l0>334
<211>62
<212>PRT
<213>谷氨酸棒状杆菌
<400>334
Val Ala Phe Ala Glu Gly Tyr Tyr Gln Glu Met Ala Glu Val Val Cys
1 5 10 15
Glu Ala Arg Glu Glu Gly Ile Thr His Glu Asp Thr Leu His Met Leu
20 25 30
Asn Asp Met Met Ala Met Lys Arg Ser Gly Glu Val Leu Asn Thr Leu
35 40 45
Ala Asp Val Leu Tyr Ala Asn Glu Cys Pro Glu Tyr Arg Ile
50 55 60
<210>335
<211>1354
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1324)
<223>RXA02786
<400>335
atagaatgag gaaatatgcg cttccgcgcc gatcaaattc attgattgtt aatcggaagt 60
tttttgaaca ggtaaagcta ggggacctgt tcagtgcctg ttg gcg gat gta tta 115
Leu Ala Asp Val Leu
1 5
agg aga atg ccc atg ctg agc cac gaa gaa att gtt gcg atc gca gaa 163
Arg Arg Met Pro Met Leu Ser His Glu Glu Ile Val Ala Ile Ala Glu
10 15 20
gat ttg ctg tct aaa cgc tac ggc ggt gta caa act ctt tct gac gtg 211
Asp Leu Leu Ser Lys Arg Tyr Gly Gly Val Gln Thr Leu Ser Asp Val
25 30 35
gag cag ctc aac ggt tcc ggc acc tcc gcg gtg ctg cgt gcc agg gtg 259
Glu Gln Leu Asn Gly Ser Gly Thr Ser Ala Val Leu Arg Ala Arg Val
40 45 50
gct aac tcc cca ttc ctc caa cag cgc tcc gtg gtg ctg aag tac gtg 307
Ala Asn Ser Pro Phe Leu Gln Gln Arg Ser Val Val Leu Lys Tyr Val
55 60 65
ccc agc acc gga gac gtc ttt gat gat tct gcg ctg gtg cgt gaa atc 355
Pro Ser Thr Gly Asp Val Phe Asp Asp Ser Ala Leu Val Arg Glu Ile
70 75 80 85
gtc tcc tac cag ttc acc acc tcc ttg tcg gaa gat gtc cgc cca ggg 403
Val Ser Tyr Gln Phe Thr Thr Ser Leu Ser Glu Asp Val Arg Pro Gly
90 95 100
cca gtc att ttg gcc tat gac att gac aag cgc atc ctg gtc att tca 451
Pro Val Ile Leu Ala Tyr Asp Ile Asp Lys Arg Ile Leu Val Ile Ser
105 110 115
gat tcc ggc aac ggc gac acc ttt gcg gat ctg atc gat cag cgt acc 499
Asp Ser Gly Asn Gly Asp Thr Phe Ala Asp Leu Ile Asp Gln Arg Thr
120 125 130
ggt gat cag cgc atg cag atc ctg cgc aac ctc ggc caa tcg ctg ggc 547
Gly Asp Gln Arg Met Gln Ile Leu Arg Asn Leu Gly Gln Ser Leu Gly
135 140 145
agc ctg cac gcc gcc acc gca gac cgc gaa gaa gac ttc aac att ctg 595
Ser Leu His Ala Ala Thr Ala Asp Arg Glu Glu Asp Phe Asn Ile Leu
150 155 160 165
ctc aac cgt atg ctg gcc aaa tac cca gcc aca gct gag atg cag aaa 643
Leu Asn Arg Met Leu Ala Lys Tyr Pro Ala Thr Ala Glu Met Gln Lys
170 175 180
aac cgc gat agg ttg ctg ccc gca gca att gag gtg ggt aaa aag ata 691
Asn Arg Asp Arg Leu Leu Pro Ala Ala Ile Glu Val Gly Lys Lys Ile
185 190 195
ctt gtc gac gcc ggg gtc acc gtt cca gaa gtc gtc gag gag ttt gcc 739
Leu Val Asp Ala Gly Val Thr Val Pro Glu Val Val Glu Glu Phe Ala
200 205 210
cgc gta gct cgg cga cgc ctg att tcc gga cgc cac cgt gcc ttc aca 787
Arg Val Ala Arg Arg Arg Leu Ile Ser Gly Arg His Arg Ala Phe Thr
215 220 225
cct ttt gac ttg tca ccc gac aac atc atc gtg gct gaa cgc acc cac 835
Pro Phe Asp Leu Ser Pro Asp Asn Ile Ile Val Ala Glu Arg Thr His
230 235 240 245
ttc ctg gac tac gaa gtt gcc ggt ttc agg gat gcc acc ttt gat gtt 883
Phe Leu Asp Tyr Glu Val Ala Gly Phe Arg Asp Ala Thr Phe Asp Val
250 255 260
gct tgt gtg atc gca ggt ttc ccg caa ttc gtg ttc agc cgc ccc atc 931
Ala Cys Val Ile Ala Gly Phe Pro Gln Phe Val Phe Ser Arg Pro Ile
265 270 275
tct gat gat gag gta gac gaa ctc att gaa tcc tgg gtc caa gaa gtc 979
Ser Asp Asp Glu Val Asp Glu Leu Ile Glu Ser Trp Val Gln Glu Val
280 285 290
cgt gga atc tgg ccc aat gtg aac aat gaa gag cgc ctc caa gca cgt 1027
Arg Gly Ile Trp Pro Asn Val Asn Asn Glu Glu Arg Leu Gln Ala Arg
295 300 305
atc gtg acc gct ctc atc ggc tgg gca cta tcg agt gtt gcc ttc atg 1075
Ile Val Thr Ala Leu Ile Gly Trp Ala Leu Ser Ser Val Ala Phe Met
310 315 320 325
aag ctc ggc tcc atc agc ggc atg ctt aac ctg ctg cac gtc acc gaa 1123
Lys Leu Gly Ser Ile Ser Gly Met Leu Asn Leu Leu His Val Thr Glu
330 335 340
gat gga aac acc act ttg gat gtc tcc aac ctt gat gaa ctg ttg att 1171
Asp Gly Asn Thr Thr Leu Asp Val Ser Asn Leu Asp Glu Leu Leu Ile
345 350 355
cca cga tct gcc gaa gat gac gag ctg gtc cag caa gac ctc cac gac 1219
Pro Arg Ser Ala Glu Asp Asp Glu Leu Val Gln Gln Asp Leu His Asp
360 365 370
acg ttc tcc gcg ctt cag cga ttt gca gcg cgc ggg ctg gat tcc agg 1267
Thr Phe Ser Ala Leu Gln Arg Phe Ala Ala Arg Gly Leu Asp Ser Arg
375 380 385
ttc cct gag gtg gca cga ttc gca gac gac gtg gtg cgt ttg ttc ctc 1315
Phe Pro Glu Val Ala Arg Phe Ala Asp Asp Val Val Arg Leu Phe Leu
390 395 400 405
aaa aat gac tagctgaaga gcaccggttg cttggttgtt 1354
Lys Asn Asp
<210>336
<211>408
<212>PRT
<213>谷氨酸棒状杆菌
<400>336
Leu Ala Asp Val Leu Arg Arg Met Pro Met Leu Ser His Glu Glu Ile
1 5 10 15
Val Ala Ile Ala Glu Asp Leu Leu Ser Lys Arg Tyr Gly Gly Val Gln
20 25 30
Thr Leu Ser Asp Val Glu Gln Leu Asn Gly Ser Gly Thr Ser Ala Val
35 40 45
Leu Arg Ala Arg Val Ala Asn Ser Pro Phe Leu Gln Gln Arg Ser Val
50 55 60
Val Leu Lys Tyr Val Pro Ser Thr Gly Asp Val Phe Asp Asp Ser Ala
65 70 75 80
Leu Val Arg Glu Ile Val Ser Tyr Gln Phe Thr Thr Ser Leu Ser Glu
85 90 95
Asp Val Arg Pro Gly Pro Val Ile Leu Ala Tyr Asp Ile Asp Lys Arg
100 105 110
Ile Leu Val Ile Ser Asp Ser Gly Asn Gly Asp Thr Phe Ala Asp Leu
115 120 125
Ile Asp Gln Arg Thr Gly Asp Gln Arg Met Gln Ile Leu Arg Asn Leu
130 135 140
Gly Gln Ser Leu Gly Ser Leu His Ala Ala Thr Ala Asp Arg Glu Glu
145 150 155 160
Asp Phe Asn Ile Leu Leu Asn Arg Met Leu Ala Lys Tyr Pro Ala Thr
165 170 175
Ala Glu Met Gln Lys Asn Arg Asp Arg Leu Leu Pro Ala Ala Ile Glu
180 185 190
Val Gly Lys Lys Ile Leu Val Asp Ala Gly Val Thr Val Pro Glu Val
195 200 205
Val Glu Glu Phe Ala Arg Val Ala Arg Arg Arg Leu Ile Ser Gly Arg
210 215 220
His Arg Ala Phe Thr Pro Phe Asp Leu Ser Pro Asp Asn Ile Ile Val
225 230 235 240
Ala Glu Arg Thr His Phe Leu Asp Tyr Glu Val Ala Gly Phe Arg Asp
245 250 255
Ala Thr Phe Asp Val Ala Cys Val Ile Ala Gly Phe Pro Gln Phe Val
260 265 270
Phe Ser Arg Pro Ile Ser Asp Asp Glu Val Asp Glu Leu Ile Glu Ser
275 280 285
Trp Val Gln Glu Val Arg Gly Ile Trp Pro Asn Val Asn Asn Glu Glu
290 295 300
Arg Leu Gln Ala Arg Ile Val Thr Ala Leu Ile Gly Trp Ala Leu Ser
305 310 315 320
Ser Val Ala Phe Met Lys Leu Gly Ser Ile Ser Gly Met Leu Asn Leu
325 330 335
Leu His Val Thr Glu Asp Gly Asn Thr Thr Leu Asp Val Ser Asn Leu
340 345 350
Asp Glu Leu Leu Ile Pro Arg Ser Ala Glu Asp Asp Glu Leu Val Gln
355 360 365
Gln Asp Leu His Asp Thr Phe Ser Ala Leu Gln Arg Phe Ala Ala Arg
370 375 380
Gly Leu Asp Ser Arg Phe Pro Glu Val Ala Arg Phe Ala Asp Asp Val
385 390 395 400
Val Arg Leu Phe Leu Lys Asn Asp
405
<210>337
<211>1555
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1525)
<223>RXA02798
<400>337
gcatcaaaat catgtgcatt catcattgct gatcatcata gacctgttgg caaatgaatt 60
caccaattct ttaaagctct caccctcttt agggaactga atg cgg tct gta ctc 115
Met Arg Ser Val Leu
1 5
gac tac tgg atc atg act ctt gca cct tcc acc aat gcg gtt tcg cag 163
Asp Tyr Trp Ile Met Thr Leu Ala Pro Ser Thr Asn Ala Val Ser Gln
10 15 20
caa aca aga agg aac cct tcc caa gcg ccg cga ctt ctt cat cga ctt 211
Gln Thr Arg Arg Asn Pro Ser Gln Ala Pro Arg Leu Leu His Arg Leu
25 30 35
cac ttc ttt gcc ggc att atc tgc gca ccg ttg att ttc atc gct gcg 259
His Phe Phe Ala Gly Ile Ile Cys Ala Pro Leu Ile Phe Ile Ala Ala
40 45 50
ctc acc gga ctg gtg tat gcg ttc tcc ccc acc att gaa agc atc tct 307
Leu Thr Gly Leu Val Tyr Ala Phe Ser Pro Thr Ile Glu Ser Ile Ser
55 60 65
aat cag gaa atg ctc acg gtc tcc aag tcg gct agc gac aca gct ctt 355
Asn Gln Glu Met Leu Thr Val Ser Lys Ser Ala Ser Asp Thr Ala Leu
70 75 80 85
ccg gtg cgt gaa caa gtg agc atc gcc caa gaa ctc cac ccc gat tta 403
Pro Val Arg Glu Gln Val Ser Ile Ala Gln Glu Leu His Pro Asp Leu
90 95 100
gat ctc tca ggt gtc cgg ctt ggc gat gat tcc tcc acc acc cgc gtc 451
Asp Leu Ser Gly Val Arg Leu Gly Asp Asp Ser Ser Thr Thr Arg Val
105 110 115
ctc ttc gcc gac gag acg ctt gct gaa tcc aca gtc cgc gcc gtg ttt 499
Leu Phe Ala Asp Glu Thr Leu Ala Glu Ser Thr Val Arg Ala Val Phe
120 125 130
gtt gat ccc tac acc ggc gag atc acc ggt gat acc acc caa tac gga 547
Val Asp Pro Tyr Thr Gly Glu Ile Thr Gly Asp Thr Thr Gln Tyr Gly
135 140 145
agc tca gct gca ctg ccg ttt cgg caa tgg gtc tcg caa ggt cac cgc 595
Ser Ser Ala Ala Leu Pro Phe Arg Gln Trp Val Ser Gln Gly His Arg
150 155 160 165
atg ttg tgg ctt ggt gaa cct gga cgt att tat tcc gaa ctt gcg gca 643
Met Leu Trp Leu Gly Glu Pro Gly Arg Ile Tyr Ser Glu Leu Ala Ala
170 175 180
agt tgg ctc gga gtt tta gcc gta ggc ggt ttc gca tta ttg tgg ttg 691
Ser Trp Leu Gly Val Leu Ala Val Gly Gly Phe Ala Leu Leu Trp Leu
185 190 195
cgc aac aaa aag ccc ggc cgc ttg aga aag atg gtg cga act ggt ggc 739
Arg Asn Lys Lys Pro Gly Arg Leu Arg Lys Met Val Arg Thr Gly Gly
200 205 210
cgt ggt cga gtg aaa acg tat cgc cga cat gca gca ttg ggc acc gta 787
Arg Gly Arg Val Lys Thr Tyr Arg Arg His Ala Ala Leu Gly Thr Val
215 220 225
gcg ggc ttg ggt ttt gtg ttc ctc acc ttt act ggc ctg acc tgg tca 835
Ala Gly Leu Gly Phe Val Phe Leu Thr Phe Thr Gly Leu Thr Trp Ser
230 235 240 245
act tat gcg gga tcc aac atc acg gat tta cgc acc caa ctc aat tgg 883
Thr Tyr Ala Gly Ser Asn Ile Thr Asp Leu Arg Thr Gln Leu Asn Trp
250 255 260
acg cag cct tcc gtt aac gct tct cta aca gct gcc cca caa gtg gac 931
Thr Gln Pro Ser Val Asn Ala Ser Leu Thr Ala Ala Pro Gln Val Asp
265 270 275
atg cat gat gaa cac gca ggt cat cac atg cat atg gag tcg gca acc 979
Met His Asp Glu His Ala Gly His His Met His Met Glu Ser Ala Thr
280 285 290
tca ggc tca ggt tct ata gat ctg gta gcg gcc acg gct atc tcc gaa 1027
Ser Gly Ser Gly Ser Ile Asp Leu Val Ala Ala Thr Ala Ile Ser Glu
295 300 305
tta cgc act ccc ctc acc att act ccc cca gca caa gat ggc ctc gcg 1075
Leu Arg Thr Pro Leu Thr Ile Thr Pro Pro Ala Gln Asp Gly Leu Ala
310 315 320 325
tgg act gcc aca gaa aac cgc gac gcc tac cga ttc acc act gac acc 1123
Trp Thr Ala Thr Glu Asn Arg Asp Ala Tyr Arg Phe Thr Thr Asp Thr
330 335 340
atc gct gtt gat ggc gac acc gga atg ctg acc aac cgt ttg aat tcc 1171
Ile Ala Val Asp Gly Asp Thr Gly Met Leu Thr Asn Arg Leu Asn Ser
345 350 355
act gat tgg cca tta gct gct caa gcg agt gca tgg ctc atc cag ctt 1219
Thr Asp Trp Pro Leu Ala Ala Gln Ala Ser Ala Trp Leu Ile Gln Leu
360 365 370
cac atg ggc acc ctg ttt ggc ctt ccc aac cag gtt gta ctt ggg ctg 1267
His Met Gly Thr Leu Phe Gly Leu Pro Asn Gln Val Val Leu Gly Leu
375 380 385
ttg gct gcc tca ata atc gtg atg atc ggc ctt ggg tat tgg atg ctg 1315
Leu Ala Ala Ser Ile Ile Val Met Ile Gly Leu Gly Tyr Trp Met Leu
390 395 400 405
tgg cag cat cgg cca cgg gaa ggt tgg cct agc gct ccc aag cgt gca 1363
Trp Gln His Arg Pro Arg Glu Gly Trp Pro Ser Ala Pro Lys Arg Ala
410 415 420
gga ttt gaa aaa ccc acc tgg gga act atc gcg ttg ggt gtt gtg gtg 1411
Gly Phe Glu Lys Pro Thr Trp Gly Thr Ile Ala Leu Gly Val Val Val
425 430 435
atc gcg tat ggg ctt ctc gcg cca ctt ttt gca gtt tca ctg ctc gtg 1459
Ile Ala Tyr Gly Leu Leu Ala Pro Leu Phe Ala Val Ser Leu Leu Val
440 445 450
ttt att gga tta agc ctt gga gtg cgg ttt att tct cgt atg tcc ggg 1507
Phe Ile Gly Leu Ser Leu Gly Val Arg Phe Ile Ser Arg Met Ser Gly
455 460 465
cgc gcg gac acg tcc aac taagaacacc aaaccacacc gcccacgaat 1555
Arg Ala Asp Thr Ser Asn
470 475
<210>338
<211>475
<212>PRT
<213>谷氨酸棒状杆菌
<400>338
Met Arg Ser Val Leu Asp Tyr Trp Ile Met Thr Leu Ala Pro Ser Thr
1 5 10 15
Asn Ala Val Ser Gln Gln Thr Arg Arg Asn Pro Ser Gln Ala Pro Arg
20 25 30
Leu Leu His Arg Leu His Phe Phe Ala Gly Ile Ile Cys Ala Pro Leu
35 40 45
Ile Phe Ile Ala Ala Leu Thr Gly Leu Val Tyr Ala Phe Ser Pro Thr
50 55 60
Ile Glu Ser Ile Ser Asn Gln Glu Met Leu Thr Val Ser Lys Ser Ala
65 70 75 80
Ser Asp Thr Ala Leu Pro Val Arg Glu Gln Val Ser Ile Ala Gln Glu
85 90 95
Leu His Pro Asp Leu Asp Leu Ser Gly Val Arg Leu Gly Asp Asp Ser
100 105 110
Ser Thr Thr Arg Val Leu Phe Ala Asp Glu Thr Leu Ala Glu Ser Thr
115 120 125
Val Arg Ala Val Phe Val Asp Pro Tyr Thr Gly Glu Ile Thr Gly Asp
130 135 140
Thr Thr Gln Tyr Gly Ser Ser Ala Ala Leu Pro Phe Arg Gln Trp Val
145 150 155 160
Ser Gln Gly His Arg Met Leu Trp Leu Gly Glu Pro Gly Arg Ile Tyr
165 170 175
Ser Glu Leu Ala Ala Ser Trp Leu Gly Val Leu Ala Val Gly Gly Phe
180 185 190
Ala Leu Leu Trp Leu Arg Asn Lys Lys Pro Gly Arg Leu Arg Lys Met
195 200 205
Val Arg Thr Gly Gly Arg Gly Arg Val Lys Thr Tyr Arg Arg His Ala
210 215 220
Ala Leu Gly Thr Val Ala Gly Leu Gly Phe Val Phe Leu Thr Phe Thr
225 230 235 240
Gly Leu Thr Trp Ser Thr Tyr Ala Gly Ser Asn Ile Thr Asp Leu Arg
245 250 255
Thr Gln Leu Asn Trp Thr Gln Pro Ser Val Asn Ala Ser Leu Thr Ala
260 265 270
Ala Pro Gln Val Asp Met His Asp Glu His Ala Gly His His Met His
275 280 285
Met Glu Ser Ala Thr Ser Gly Ser Gly Ser Ile Asp Leu Val Ala Ala
290 295 300
Thr Ala Ile Ser Glu Leu Arg Thr Pro Leu Thr Ile Thr Pro Pro Ala
305 310 315 320
Gln Asp Gly Leu Ala Trp Thr Ala Thr Glu Asn Arg Asp Ala Tyr Arg
325 330 335
Phe Thr Thr Asp Thr Ile Ala Val Asp Gly Asp Thr Gly Met Leu Thr
340 345 350
Asn Arg Leu Asn Ser Thr Asp Trp Pro Leu Ala Ala Gln Ala Ser Ala
355 360 365
Trp Leu Ile Gln Leu His Met Gly Thr Leu Phe Gly Leu Pro Asn Gln
370 375 380
Val Val Leu Gly Leu Leu Ala Ala Ser Ile Ile Val Met Ile Gly Leu
385 390 395 400
Gly Tyr Trp Met Leu Trp Gln His Arg Pro Arg Glu Gly Trp Pro Ser
405 410 415
Ala Pro Lys Arg Ala Gly Phe Glu Lys Pro Thr Trp Gly Thr Ile Ala
420 425 430
Leu Gly Val Val Val Ile Ala Tyr Gly Leu Leu Ala Pro Leu Phe Ala
435 440 445
Val Ser Leu Leu Val Phe Ile Gly Leu Ser Leu Gly Val Arg Phe Ile
450 455 460
Ser Arg Met Ser Gly Arg Ala Asp Thr Ser Asn
465 470 475
<210>339
<211>1729
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<22l>CDS
<222>(101)..(1699)
<223>RXA02817
<400>339
aaaattccca cccccaaaac tcccccactt cggttaagga atcaggattc tcacaaagtt 60
caggcaggct cccgctactt ttcagcgcta atcttggctc atg att tta ggc gta 115
Met Ile Leu Gly Val
1 5
ccc att caa tat ttg ctc tat tca ttg tgg aat tgg att gtc gat acc 163
Pro Ile Gln Tyr Leu Leu Tyr Ser Leu Trp Asn Trp Ile Val Asp Thr
10 15 20
ggt ttt gat gta gca att atc ctg gtc ttg gcg ttt ttg att cca cgt 211
Gly Phe Asp Val Ala Ile Ile Leu Val Leu Ala Phe Leu Ile Pro Arg
25 30 35
atc ggc cga ctg gcc atg cgt att atc aag cgc cga gtg gag tct gca 259
Ile Gly Arg Leu Ala Met Arg Ile Ile Lys Arg Arg Val Glu Ser Ala
40 45 50
gcc gat gcg gac acc act aag aac cag ctc gcg ttc gcc ggc gtt ggc 307
Ala Asp Ala Asp Thr Thr Lys Asn Gln Leu Ala Phe Ala Gly Val Gly
55 60 65
gtt tat atc gcg caa att gtg gcg ttt ttc atg ctt gcc gtc tcc gcg 355
Val Tyr Ile Ala Gln Ile Val Ala Phe Phe Met Leu Ala Val Ser Ala
70 75 80 85
atg cag gct ttt ggt ttc tct ctc gcg ggc gct gcg att ccg gca acc 403
Met Gln Ala Phe Gly Phe Ser Leu Ala Gly Ala Ala Ile Pro Ala Thr
90 95 100
att gcg tca gct gcc att ggc ctt ggt gcg cag tcg att gtt gcg gac 451
Ile Ala Ser Ala Ala Ile Gly Leu Gly Ala Gln Ser Ile Val Ala Asp
105 110 115
ttc ttg gcc gga ttt ttc atc ctg acg gaa aag caa ttc ggc gtg ggt 499
Phe Leu Ala Gly Phe Phe Ile Leu Thr Glu Lys Gln Phe Gly Val Gly
120 125 130
gac tgg gtg cgt ttt gag ggc aac ggc atc gtt gtc gaa ggc acc gtc 547
Asp Trp Val Arg Phe Glu Gly Asn Gly Ile Val Val Glu Gly Thr Val
135 140 145
att gag atc acc atg cgc gcg acc aaa att cgc acg att gca caa gag 595
Ile Glu Ile Thr Met Arg Ala Thr Lys Ile Arg Thr Ile Ala Gln Glu
150 155 160 165
acc gtg atc atc ccc aac tcc acg gcg aaa gtg tgc atc aac aat tct 643
Thr Val Ile Ile Pro Asn Ser Thr Ala Lys Val Cys Ile Asn Asn Ser
170 175 180
aat aac tgg tcg cgt gcg gtt gtc gtt att ccg atc ccc atg ttg ggt 691
ASn Asn Trp Ser Arg Ala Val Val Val Ile Pro Ile Pro Met Leu Gly
185 190 195
tct gaa aac atc aca gat gtc atc gcg cgc tct gaa gct gcg act cgt 739
Ser Glu Asn Ile Thr Asp Val Ile Ala Arg Ser Glu Ala Ala Thr Arg
200 205 210
cgc gca ctt ggc cag gag aaa atc gca ccg gaa atc ctc ggt gaa ctc 787
Arg Ala Leu Gly Gln Glu Lys Ile Ala Pro Glu Ile Leu Gly Glu Leu
215 220 225
gat gtg cac cca gcc acg gaa gtc acg ccg cca acg gtg gtc ggc atg 835
Asp Val His Pro Ala Thr Glu Val Thr Pro Pro Thr Val Val Gly Met
230 235 240 245
ccg tgg atg gtc acc atg cgt ttc ctc gtg caa gtc acc gcc ggc aat 883
Pro Trp Met Val Thr Met Arg Phe Leu Val Gln Val Thr Ala Gly Asn
250 255 260
caa tgg ctg gtc gaa cgc gcc atc cgc aca gaa atc atc agc gaa ttc 931
Gln Trp Leu Val Glu Arg Ala Ile Arg Thr Glu Ile Ile Ser Glu Phe
265 270 275
tgg gaa gaa tac ggc agc gca acc act aca tcg gga acc ctc att gat 979
Trp Glu Glu Tyr Gly Ser Ala Thr Thr Thr Ser Gly Thr Leu Ile Asp
280 285 290
tcc tta cac gtt gag cat gaa gag cca aag acc tcg ctt atc gac gcc 1027
Ser Leu His Val Glu His Glu Glu Pro Lys Thr Ser Leu Ile Asp Ala
295 300 305
tcc ccc cag gct ctt aag gaa ccg aag ccg gag gct gcg gcg acg gtt 1075
Ser Pro Gln Ala Leu Lys Glu Pro Lys Pro Glu Ala Ala Ala Thr Val
310 315 320 325
gca tcg cta gct gca tcc tct aac gac gat gca gac aat gca gac gcc 1123
Ala Ser Leu Ala Ala Ser Ser Asn Asp Asp Ala Asp Asn Ala Asp Ala
330 335 340
tcg gtg atc aat gca ggc aat cca gag aag gaa ctt gat tcc gat gtg 1171
Ser Val Ile Asn Ala Gly Asn Pro Glu Lys Glu Leu Asp Ser Asp Val
345 350 355
ctg gaa caa gaa ctc tcc agc gaa gaa ccg gaa gaa aca gca aaa cca 1219
Leu Glu Gln Glu Leu Ser Ser Glu Glu Pro Glu Glu Thr Ala Lys Pro
360 365 370
gat cac tct ctc cga ggc ttc ttc cgc act gat tac tac cca aat cgg 1267
Asp His Ser Leu Arg Gly Phe Phe Arg Thr Asp Tyr Tyr Pro Asn Arg
375 380 385
tgg cag aag atc ctg tcg ttt ggc gga cgt gtc cgc atg agc acg tcc 1315
Trp Gln Lys Ile Leu Ser Phe Gly Gly Arg Val Arg Met Ser Thr Ser
390 395 400 405
ctg ttg ttg ggt gcg ctg ctc ttg ctg tca cta ttt aag gtc atg act 1363
Leu Leu Leu Gly Ala Leu Leu Leu Leu Ser Leu Phe Lys Val Met Thr
410 415 420
gtg gaa cca agt gag aat tgg caa aac tcc agt gga tgg ctg tca cca 1411
Val Glu Pro Ser Glu Asn Trp Gln Asn Ser Ser Gly Trp Leu Ser Pro
425 430 435
agc act gcc acc tca act gcg gtg acc acc tcc gaa act tcc gcg cca 1459
Ser Thr Ala Thr Ser Thr Ala Val Thr Thr Ser Glu Thr Ser Ala Pro
440 445 450
gta agc acg cct tcg atg aca gtg ccc act acg gtg gag gag acc cca 1507
Val Ser Thr Pro Ser Met Thr Val Pro Thr Thr Val Glu Glu Thr Pro
455 460 465
acg atg gaa tct aac gtc gaa acg cag cag gaa acc tca acc cct gca 1555
Thr Met Glu Ser Asn Val Glu Thr Gln Gln Glu Thr Ser Thr Pro Ala
470 475 480 485
acc gca acg ccc cag cga gcc gac acc atc gaa ccg acc gag gaa gcc 1603
Thr Ala Thr Pro Gln Arg Ala Asp Thr Ile Glu Pro Thr Glu Glu Ala
490 495 500
acg tcg cag gag gaa acg act gcg tcg cag acg cag tct cca gca gtg 1651
Thr Ser Gln Glu Glu Thr Thr Ala Ser Gln Thr Gln Ser Pro Ala Val
505 510 515
gaa gca cca acc gcg gtc caa gag aca gtt gcg ccg acg tcc acc cct 1699
Glu Ala Pro Thr Ala Val Gln Glu Thr Val Ala Pro Thr Ser Thr Pro
520 525 530
taggacgctg attacagacg tgtcccattt 1729
<210>340
<211>533
<212>PRT
<213>谷氨酸棒状杆菌
<400>340
Met Ile Leu Gly Val Pro Ile Gln Tyr Leu Leu Tyr Ser Leu Trp Asn
1 5 10 15
Trp Ile Val Asp Thr Gly Phe Asp Val Ala Ile Ile Leu Val Leu Ala
20 25 30
Phe Leu Ile Pro Arg Ile Gly Arg Leu Ala Met Arg Ile Ile Lys Arg
35 40 45
Arg Val Glu Ser Ala Ala Asp Ala Asp Thr Thr Lys Asn Gln Leu Ala
50 55 60
Phe Ala Gly Val Gly Val Tyr Ile Ala Gln Ile Val Ala Phe Phe Met
65 70 75 80
Leu Ala Val Ser Ala Met Gln Ala Phe Gly Phe Ser Leu Ala Gly Ala
85 90 95
Ala Ile Pro Ala Thr Ile Ala Ser Ala Ala Ile Gly Leu Gly Ala Gln
100 105 110
Ser Ile Val Ala Asp Phe Leu Ala Gly Phe Phe Ile Leu Thr Glu Lys
115 120 125
Gln Phe Gly Val Gly Asp Trp Val Arg Phe Glu Gly Asn Gly Ile Val
130 135 140
Val Glu Gly Thr Val Ile Glu Ile Thr Met Arg Ala Thr Lys Ile Arg
145 150 155 160
Thr Ile Ala Gln Glu Thr Val Ile Ile Pro Asn Ser Thr Ala Lys Val
165 170 175
Cys Ile Asn Asn Ser Asn Asn Trp Ser Arg Ala Val Val Val Ile Pro
180 185 190
Ile Pro Met Leu Gly Ser Glu Asn Ile Thr Asp Val Ile Ala Arg Ser
195 200 205
Glu Ala Ala Thr Arg Arg Ala Leu Gly Gln Glu Lys Ile Ala Pro Glu
210 215 220
Ile Leu Gly Glu Leu Asp Val His Pro Ala Thr Glu Val Thr Pro Pro
225 230 235 240
Thr Val Val Gly Met Pro Trp Met Val Thr Met Arg Phe Leu Val Gln
245 250 255
Val Thr Ala Gly Asn Gln Trp Leu Val Glu Arg Ala Ile Arg Thr Glu
260 265 270
Ile Ile Ser Glu Phe Trp Glu Glu Tyr Gly Ser Ala Thr Thr Thr Ser
275 280 285
Gly Thr Leu Ile Asp Ser Leu His Val Glu His Glu Glu Pro Lys Thr
290 295 300
Ser Leu Ile Asp Ala Ser Pro Gln Ala Leu Lys Glu Pro Lys Pro Glu
305 310 315 320
Ala Ala Ala Thr Val Ala Ser Leu Ala Ala Ser Ser Asn Asp Asp Ala
325 330 335
Asp Asn Ala Asp Ala Ser Val Ile Asn Ala Gly Asn Pro Glu Lys Glu
340 345 350
Leu Asp Ser Asp Val Leu Glu Gln Glu Leu Ser Ser Glu Glu Pro Glu
355 360 365
Glu Thr Ala Lys Pro Asp His Ser Leu Arg Gly Phe Phe Arg Thr Asp
370 375 380
Tyr Tyr Pro Asn Arg Trp Gln Lys Ile Leu Ser Phe Gly Gly Arg Val
385 390 395 400
Arg Met Ser Thr Ser Leu Leu Leu Gly Ala Leu Leu Leu Leu Ser Leu
405 410 415
Phe Lys Val Met Thr Val Glu Pro Ser Glu Asn Trp Gln Asn Ser Ser
420 425 430
Gly Trp Leu Ser Pro Ser Thr Ala Thr Ser Thr Ala Val Thr Thr Ser
435 440 445
Glu Thr Ser Ala Pro Val Ser Thr Pro Ser Met Thr Val Pro Thr Thr
450 455 460
Val Glu Glu Thr Pro Thr Met Glu Ser Asn Val Glu Thr Gln Gln Glu
465 470 475 480
Thr Ser Thr Pro Ala Thr Ala Thr Pro Gln Arg Ala Asp Thr Ile Glu
485 490 495
Pro Thr Glu Glu Ala Thr Ser Gln Glu Glu Thr Thr Ala Ser Gln Thr
500 505 510
Gln Ser Pro Ala Val Glu Ala Pro Thr Ala Val Gln Glu Thr Val Ala
515 520 525
Pro Thr Ser Thr Pro
530
<210>341
<211>1969
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1939)
<223>RXA02825
<400>341
tggcctgccc ctgaaacttt tacggctttc agagcgcagg gcatcatttt cttgtgtcgc 60
caacacttga gaaaaattgc ggaaaaggac actgctgttc atg aaa ctt gca cct 115
Met Lys Leu Ala Pro
1 5
cgt atg cgg atg agg agc ccc aaa act ttc gcg gcc ctc gcc tca ctt 163
Arg Met Arg Met Arg Ser Pro Lys Thr Phe Ala Ala Leu Ala Ser Leu
10 15 20
gct tta gtc ata ggt ctc ggc cag gta ccg atc gcc caa gct caa acc 211
Ala Leu Val Ile Gly Leu Gly Gln Val Pro Ile Ala Gln Ala Gln Thr
25 30 35
gag tat cga acc gcc tcc gac ggt tcc ctg aac tgg gga ttt agg caa 259
Glu Tyr Arg Thr Ala Ser Asp Gly Ser Leu Asn Trp Gly Phe Arg Gln
40 45 50
tcg ttc cgc aat tac atc caa acc ggc gtg gcc aaa ggt tcc atc acg 307
Ser Phe Arg Asn Tyr Ile Gln Thr Gly Val Ala Lys Gly Ser Ile Thr
55 60 65
ctt ggc gac ggc gca tcc gac aac ggt ggc aac ttc gca ttc acc cca 355
Leu Gly Asp Gly Ala Ser Asp Asn Gly Gly Asn Phe Ala Phe Thr Pro
70 75 80 85
cgc acc aac ggc acc acc gtg acc agc gat tcc caa ggc acc gtg gaa 403
Arg Thr Asn Gly Thr Thr Val Thr Ser Asp Ser Gln Gly Thr Val Glu
90 95 100
ttc aac ggc tcc gtg cac ttc ctc gga cac cag gca gag gac aaa tgg 451
Phe Asn Gly Ser Val His Phe Leu Gly His Gln Ala Glu Asp Lys Trp
105 110 115
atc ctg gac acc acc atg tct gac atc aaa atg gtg ttc aac gga tcc 499
Ile Leu Asp Thr Thr Met Ser Asp Ile Lys Met Val Phe Asn Gly Ser
120 125 130
tcc gcg cag cta gtt gtg gat ttg gtt gcc cgc gaa ttc aag ggc acc 547
Ser Ala Gln Leu Val Val Asp Leu Val Ala Arg Glu Phe Lys Gly Thr
135 140 145
acc tac gat gac atc ggc gaa tac atc atc tcc gac gac atc gtg ctt 595
Thr Tyr Asp Asp Ile Gly Glu Tyr Ile Ile Ser Asp Asp Ile Val Leu
150 155 160 165
gcc gac gtc tcc ctc aac tcc gcc gcc gac ttc tcc caa gat tcc atc 643
Ala Asp Val Ser Leu Asn Ser Ala Ala Asp Phe Ser Gln Asp Ser Ile
170 175 180
gac ctg tcc ggc acc acc gac ctc acc gca gct ggc gcc caa gct ttc 691
Asp Leu Ser Gly Thr Thr Asp Leu Thr Ala Ala Gly Ala Gln Ala Phe
185 190 195
gga gga ttc tac gaa acc ggc gaa gcc ctc gac ccg acc ggc ggc agc 739
Gly Gly Phe Tyr Glu Thr Gly Glu Ala Leu Asp Pro Thr Gly Gly Ser
200 205 210
ctg acc att tcc tcc acc acc acc gcg cca tcg acc agc acg acc tcc 787
Leu Thr Ile Ser Ser Thr Thr Thr Ala Pro Ser Thr Ser Thr Thr Ser
215 220 225
acc tct gcc tca act tcc ggt gga acc gcc gac tgt tcc tcc ggc gca 835
Thr Ser Ala Ser Thr Ser Gly Gly Thr Ala Asp Cys Ser Ser Gly Ala
230 235 240 245
ttg ggt gtt gtc acc acc gga acc aac gac ggc atg ctg ggc acc atc 883
Leu Gly Val Val Thr Thr Gly Thr Asn Asp Gly Met Leu Gly Thr Ile
250 255 260
cag gaa gta aac aac acc ttc gcg att tgg aac aac ctc atc gtc aac 931
Gln Glu Val Asn Asn Thr Phe Ala Ile Trp Asn Asn Leu Ile Val Asn
265 270 275
acc gag cgc atg ttc tgc aac att gat acc ctc aag gcg cgc ttc gac 979
Thr Glu Arg Met Phe Cys Asn Ile Asp Thr Leu Lys Ala Arg Phe Asp
280 285 290
acg gat gat tcc agc gat tca gcg acc tct gcg act tct ggg act act 1027
Thr Asp Asp Ser Ser Asp Ser Ala Thr Ser Ala Thr Ser Gly Thr Thr
295 300 305
gcg tcc acc ggc acc acc gct gca act acc gcg gga acc acg ggt acc 1075
Ala Ser Thr Gly Thr Thr Ala Ala Thr Thr Ala Gly Thr Thr Gly Thr
310 315 320 325
act gga act gcc agc acc gct tcc gga act tcc gga act tcc gga acc 1123
Thr Gly Thr Ala Ser Thr Ala Ser Gly Thr Ser Gly Thr Ser Gly Thr
330 335 340
tcc ggc acc gca gca act gtc gct ggc acc acc cca act gac aat ggc 1171
Ser Gly Thr Ala Ala Thr Val Ala Gly Thr Thr Pro Thr Asp Asn Gly
345 350 355
gtt tgc acc gct tcc gga tct ttg ggc gtg acc caa gca tct gcg cag 1219
Val Cys Thr Ala Ser Gly Ser Leu Gly Val Thr Gln Ala Ser Ala Gln
360 365 370
tgg ggt gtg aag gcg tcc ttc cag aac tac att cgc gga tcg atc gcc 1267
Trp Gly Val Lys Ala Ser Phe Gln Asn Tyr Ile Arg Gly Ser Ile Ala
375 380 385
aac ggt agc tgg act ctc aac ggc gtt ggt ttt gat aat cag cag ttc 1315
Asn Gly Ser Trp Thr Leu Asn Gly Val Gly Phe Asp Asn Gln Gln Phe
390 395 400 405
caa ttc tct gga aat tcc gga gca gtc gac gcg gaa aac aag acc ggc 1363
Gln Phe Ser Gly Asn Ser Gly Ala Val Asp Ala Glu Asn Lys Thr Gly
410 415 420
agc atc aat ttc cct ggt tcc atc cac ttc acg ggt cac ggc gga atc 1411
Ser Ile Asn Phe Pro Gly Ser Ile His Phe Thr Gly His Gly Gly Ile
425 430 435
ttg gac atg cag atc gca aac att gag atc agc ttc aac ggc aac tcc 1459
Leu Asp Met Gln Ile Ala Asn Ile Glu Ile Ser Phe Asn Gly Asn Ser
440 445 450
ggc gag ctg att gcg gat gtc gtt tcc tct gac atg gat gga aat tcc 1507
Gly Glu Leu Ile Ala Asp Val Val Ser Ser Asp Met Asp Gly Asn Ser
455 460 465
acc aac tac ggt cgc act gtc gtg ggc acc ctg aac ttc tct gcg ttg 1555
Thr Asn Tyr Gly Arg Thr Val Val Gly Thr Leu Asn Phe Ser Ala Leu
470 475 480 485
aat gtt tct gca acg gaa gct tcc ggt tcc gct tcg gtg tcc ctg tca 1603
Asn Val Ser Ala Thr Glu Ala Ser Gly Ser Ala Ser Val Ser Leu Ser
490 495 500
cag tcg ggt tcg cag gcg ttc gct gat ttc tac acc cca ggc acc cag 1651
Gln Ser Gly Ser Gln Ala Phe Ala Asp Phe Tyr Thr Pro Gly Thr Gln
505 510 515
ttg gat ccg atc agt ttc agc gca act ttg ggc ggc gac gcc agc tgc 1699
Leu Asp Pro Ile Ser Phe Ser Ala Thr Leu Gly Gly Asp Ala Ser Cys
520 525 530
gcc acc gga tcc acc tcg acc aca ggc gct gct gcc acc gcg aac act 1747
Ala Thr Gly Ser Thr Ser Thr Thr Gly Ala Ala Ala Thr Ala Asn Thr
535 540 545
gac aac acc gaa ggt gtt gcc ggc gag gaa tcc acc acc ccc gct aac 1795
Asp Asn Thr Glu Gly Val Ala Gly Glu Glu Ser Thr Thr Pro Ala Asn
550 555 560 565
caa aac agc cag ttc caa atc cgc cag gcc gct gca gat tcc acc gga 1843
Gln Asn Ser Gln Phe Gln Ile Arg Gln Ala Ala Ala Asp Ser Thr Gly
570 575 580
ctg gat acc acc acc aca atg ttg ctc atc ctc gcg gcg ttc gtt gtc 1891
Leu Asp Thr Thr Thr Thr Met Leu Leu Ile Leu Ala Ala Phe Val Val
585 590 595
gca ggt ggc tcc atg act cgc ttc acc gtc ggc aac ccg act gga aaa 1939
Ala Gly Gly Ser Met Thr Arg Phe Thr Val Gly Asn Pro Thr Gly Lys
600 605 610
taaggcttca catgaataac gcttttcgac 1969
<210>342
<211>613
<212>PRT
<213>谷氨酸棒状杆菌
<400>342
Met Lys Leu Ala Pro Arg Met Arg Met Arg Ser Pro Lys Thr Phe Ala
1 5 10 15
Ala Leu Ala Ser Leu Ala Leu Val Ile Gly Leu Gly Gln Val Pro Ile
20 25 30
Ala Gln Ala Gln Thr Glu Tyr Arg Thr Ala Ser Asp Gly Ser Leu Asn
35 40 45
Trp Gly Phe Arg Gln Ser Phe Arg Asn Tyr Ile Gln Thr Gly Val Ala
50 55 60
Lys Gly Ser Ile Thr Leu Gly Asp Gly Ala Ser Asp Asn Gly Gly Asn
65 70 75 80
Phe Ala Phe Thr Pro Arg Thr Asn Gly Thr Thr Val Thr Ser Asp Ser
85 90 95
Gln Gly Thr Val Glu Phe Asn Gly Ser Val His Phe Leu Gly His Gln
100 105 110
Ala Glu Asp Lys Trp Ile Leu Asp Thr Thr Met Ser Asp Ile Lys Met
115 120 125
Val Phe Asn Gly Ser Ser Ala Gln Leu Val Val Asp Leu Val Ala Arg
130 135 140
Glu Phe Lys Gly Thr Thr Tyr Asp Asp Ile Gly Glu Tyr Ile Ile Ser
145 150 155 160
Asp Asp Ile Val Leu Ala Asp Val Ser Leu Asn Ser Ala Ala Asp Phe
165 170 175
Ser Gln Asp Ser Ile Asp Leu Ser Gly Thr Thr Asp Leu Thr Ala Ala
180 185 190
Gly Ala Gln Ala Phe Gly Gly Phe Tyr Glu Thr Gly Glu Ala Leu Asp
195 200 205
Pro Thr Gly Gly Ser Leu Thr Ile Ser Ser Thr Thr Thr Ala Pro Ser
210 215 220
Thr Ser Thr Thr Ser Thr Ser Ala Ser Thr Ser Gly Gly Thr Ala Asp
225 230 235 240
Cys Ser Ser Gly Ala Leu Gly Val Val Thr Thr Gly Thr Asn Asp Gly
245 250 255
Met Leu Gly Thr Ile Gln Glu Val Asn Asn Thr Phe Ala Ile Trp Asn
260 265 270
Asn Leu Ile Val Asn Thr Glu Arg Met Phe Cys Asn Ile Asp Thr Leu
275 280 285
Lys Ala Arg Phe Asp Thr Asp Asp Ser Ser Asp Ser Ala Thr Ser Ala
290 295 300
Thr Ser Gly Thr Thr Ala Ser Thr Gly Thr Thr Ala Ala Thr Thr Ala
305 310 315 320
Gly Thr Thr Gly Thr Thr Gly Thr Ala Ser Thr Ala Ser Gly Thr Ser
325 330 335
Gly Thr Ser Gly Thr Ser Gly Thr Ala Ala Thr Val Ala Gly Thr Thr
340 345 350
Pro Thr Asp Asn Gly Val Cys Thr Ala Ser Gly Ser Leu Gly Val Thr
355 360 365
Gln Ala Ser Ala Gln Trp Gly Val Lys Ala Ser Phe Gln Asn Tyr Ile
370 375 380
Arg Gly Ser Ile Ala Asn Gly Ser Trp Thr Leu Asn Gly Val Gly Phe
385 390 395 400
Asp Asn Gln Gln Phe Gln Phe Ser Gly Asn Ser Gly Ala Val Asp Ala
405 410 415
Glu Asn Lys Thr Gly Ser Ile Asn Phe Pro Gly Ser Ile His Phe Thr
420 425 430
Gly His Gly Gly Ile Leu Asp Met Gln Ile Ala Asn Ile Glu Ile Ser
435 440 445
Phe Asn Gly Asn Ser Gly Glu Leu Ile Ala Asp Val Val Ser Ser Asp
450 455 460
Met Asp Gly Asn Ser Thr Asn Tyr Gly Arg Thr Val Val Gly Thr Leu
465 470 475 480
Asn Phe Ser Ala Leu Asn Val Ser Ala Thr Glu Ala Ser Gly Ser Ala
485 490 495
Ser Val Ser Leu Ser Gln Ser Gly Ser Gln Ala Phe Ala Asp Phe Tyr
500 505 510
Thr Pro Gly Thr Gln Leu Asp Pro Ile Ser Phe Ser Ala Thr Leu Gly
515 520 525
Gly Asp Ala Ser Cys Ala Thr Gly Ser Thr Ser Thr Thr Gly Ala Ala
530 535 540
Ala Thr Ala Asn Thr Asp Asn Thr Glu Gly Val Ala Gly Glu Glu Ser
545 550 555 560
Thr Thr Pro Ala Asn Gln Asn Ser Gln Phe Gln Ile Arg Gln Ala Ala
565 570 575
Ala Asp Ser Thr Gly Leu Asp Thr Thr Thr Thr Met Leu Leu Ile Leu
580 585 590
Ala Ala Phe Val Val Ala Gly Gly Ser Met Thr Arg Phe Thr Val Gly
595 600 605
Asn Pro Thr Gly Lys
610
<210>343
<211>739
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(709)
<223>RXA02951
<400>343
gataatgagt cagaaaatga aggtagcact acttgctgga ttaattttca aggttttctt 60
tcagaaatta atccatagcc gatatttaag gtgagaacac atg agc tcc acg ctc 115
Met Ser Ser Thr Leu
1 5
gca cga aaa gct tca ggc gac ggt tcc gaa aag aag aag cct ggc ttg 163
Ala Arg Lys Ala Ser Gly Asp Gly Ser Glu Lys Lys Lys Pro Gly Leu
10 15 20
ttt aga cgt gcg ctt cgt ttc ctc acc cac cca cct cac aaa cag gta 211
Phe Arg Arg Ala Leu Arg Phe Leu Thr His Pro Pro His Lys Gln Val
25 30 35
gac aac agc ctt gcg gat atc cgt gaa gga tta ggc ctc ggg gtg gac 259
Asp Asn Ser Leu Ala Asp Ile Arg Glu Gly Leu Gly Leu Gly Val Asp
40 45 50
gat tcc cac gaa gga tcc aaa ccc agc aac gat cat tct ttg ctg cat 307
Asp Ser His Glu Gly Ser Lys Pro Ser Asn Asp His Ser Leu Leu His
55 60 65
gaa aag cct gaa att tcc gtc atg ccg acc gaa tca atg gca cgg ctg 355
Glu Lys Pro Glu Ile Ser Val Met Pro Thr Glu Ser Met Ala Arg Leu
70 75 80 85
atc ttt tat gcg cct gat atg gat ggg caa aca gac ccc ggt gaa gta 403
Ile Phe Tyr Ala Pro Asp Met Asp Gly Gln Thr Asp Pro Gly Glu Val
90 95 100
gtg tgg att tgg gca cca gcc gat ggc ccc cag caa cca ccc cgt aaa 451
Val Trp Ile Trp Ala Pro Ala Asp Gly Pro Gln Gln Pro Pro Arg Lys
105 110 115
cgt gcc atc gtt gtt gtt gga aga aac cga aac gcc att tta ggt ctg 499
Arg Ala Ile Val Val Val Gly Arg Asn Arg Asn Ala Ile Leu Gly Leu
120 125 130
ctc att tcc tgc aac ccc gag cac cgc act gat gaa gac tgg atc gac 547
Leu Ile Ser Cys Asn Pro Glu His Arg Thr Asp Glu Asp Trp Ile Asp
135 140 145
att gga tct ggc agc tgg gac cct cga ggt cgc caa agt tgg gta cga 595
Ile Gly Ser Gly Ser Trp Asp Pro Arg Gly Arg Gln Ser Trp Val Arg
150 155 160 165
ctc gac cgc gtt ctg gag gta ccc gaa ttg ggt atc cgg cgc caa gga 643
Leu Asp Arg Val Leu Glu Val Pro Glu Leu Gly Ile Arg Arg Gln Gly
170 175 180
aca gtc gtc ccc ccg ggg cgc ttt gag cgt atc gcc aac cgc ctc cgc 691
Thr Val Val Pro Pro Gly Arg Phe Glu Arg Ile Ala Asn Arg Leu Arg
185 190 195
aac gat ttc aac tgg gtc taacatttat ttggtaattg ggcaacatca 739
Asn Asp Phe Asn Trp Val
200
<210>344
<211>203
<212>PRT
<213>谷氨酸棒状杆菌
<400>344
Met Ser Ser Thr Leu Ala Arg Lys Ala Ser Gly Asp Gly Ser Glu Lys
1 5 10 15
Lys Lys Pro Gly Leu Phe Arg Arg Ala Leu Arg Phe Leu Thr His Pro
20 25 30
Pro His Lys Gln Val Asp Asn Ser Leu Ala Asp Ile Arg Glu Gly Leu
35 40 45
Gly Leu Gly Val Asp Asp Ser His Glu Gly Ser Lys Pro Ser Asn Asp
50 55 60
His Ser Leu Leu His Glu Lys Pro Glu Ile Ser Val Met Pro Thr Glu
65 70 75 80
Ser Met Ala Arg Leu Ile Phe Tyr Ala Pro Asp Met Asp Gly Gln Thr
85 90 95
Asp Pro Gly Glu Val Val Trp Ile Trp Ala Pro Ala Asp Gly Pro Gln
100 105 110
Gln Pro Pro Arg Lys Arg Ala Ile Val Val Val Gly Arg Asn Arg Asn
115 120 125
Ala Ile Leu Gly Leu Leu Ile Ser Cys Asn Pro Glu His Arg Thr Asp
130 135 140
Glu Asp Trp Ile Asp Ile Gly Ser Gly Ser Trp Asp Pro Arg Gly Arg
145 150 155 160
Gln Ser Trp Val Arg Leu Asp Arg Val Leu Glu Val Pro Glu Leu Gly
165 170 175
Ile Arg Arg Gln Gly Thr Val Val Pro Pro Gly Arg Phe Glu Arg Ile
180 185 190
Ala Asn Arg Leu Arg Asn Asp Phe Asn Trp Val
195 200
<210>345
<211>742
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(712)
<223>RXA03010
<400>345
ttatattaat caaacaggaa aatgtcagag aagaaggcat atctcaagga aacttggcag 60
ctttagagtt tcgatcaatt attcacgtgc ggagtagctg atg tca caa gga ttt 115
Met Ser Gln Gly Phe
1 5
acc caa cca ccc ggg gga acg ttt ggt tct tcc tct ggc gga ttt tca 163
Thr Gln Pro Pro Gly Gly Thr Phe Gly Ser Ser Ser Gly Gly Phe Ser
10 15 20
ggc ggg caa tca ggt ttc ggt gga ggc tcc caa aac agt ggt ttt ggt 211
Gly Gly Gln Ser Gly Phe Gly Gly Gly Ser Gln Asn Ser Gly Phe Gly
25 30 35
ggc tct tca ggc ggt ttc tct ggg ggt tca tcg aat ggc tcg ttc ggc 259
Gly Ser Ser Gly Gly Phe Ser Gly Gly Ser Ser Asn Gly Ser Phe Gly
40 45 50
ggt tca act ggc ggg ttt acc ccg gcc gga gca acg tca aac cca tcg 307
Gly Ser Thr Gly Gly Phe Thr Pro Ala Gly Ala Thr Ser Asn Pro Ser
55 60 65
ggc ttt tcg acg cca aag gtt gca gaa agc cca gcg ccg ggg cgc tca 355
Gly Phe Ser Thr Pro Lys Val Ala Glu Ser Pro Ala Pro Gly Arg Ser
70 75 80 85
tcg ggg cga aaa aga gct ggt ggg cgc ctt caa tcg gca ccc act gaa 403
Ser Gly Arg Lys Arg Ala Gly Gly Arg Leu Gln Ser Ala Pro Thr Glu
90 95 100
tgg att ctt ccg ggc cta gtt tta ggg ctg atc agc ctg gga ctc aat 451
Trp Ile Leu Pro Gly Leu Val Leu Gly Leu Ile Ser Leu Gly Leu Asn
105 110 115
gcg tgg att acc ttc ggt gcg cca gtg gcc acc gat aat ctt ttt ctc 499
Ala Trp Ile Thr Phe Gly Ala Pro Val Ala Thr Asp Asn Leu Phe Leu
120 125 130
atc gtt gct gga att gcg tgg gtg ttg gca gga att ttg agc gtg ctg 547
Ile Val Ala Gly Ile Ala Trp Val Leu Ala Gly Ile Leu Ser Val Leu
135 140 145
tcc atc ggc aag tac atg tca gca gta aat gag cga aag tcg aca ggc 595
Ser Ile Gly Lys Tyr Met Ser Ala Val Asn Glu Arg Lys Ser Thr Gly
150 155 160 165
ttt tat aca gaa gtg ccg tgg aag aaa gcg ctc ttt act gcg aca gcc 643
Phe Tyr Thr Glu Val Pro Trp Lys Lys Ala Leu Phe Thr Ala Thr Ala
170 175 180
gtc cta ttg gtg ttt gct gtt gtg tgg tcg gcc ttg gat att gcc ctg 691
Val Leu Leu Val Phe Ala Val Val Trp Ser Ala Leu Asp Ile Ala Leu
185 190 195
tgg gta gga aag cag tca tgg tgaacacgtt gaactctaaa accgtgaatg 742
Trp Val Gly Lys Gln Ser Trp
200
<210>346
<211>204
<212>PRT
<213>谷氨酸棒状杆菌
<400>346
Met Ser Gln Gly Phe Thr Gln Pro Pro Gly Gly Thr Phe Gly Ser Ser
1 5 10 15
Ser Gly Gly Phe Ser Gly Gly Gln Ser Gly Phe Gly Gly Gly Ser Gln
20 25 30
Asn Ser Gly Phe Gly Gly Ser Ser Gly Gly Phe Ser Gly Gly Ser Ser
35 40 45
Asn Gly Ser Phe Gly Gly Ser Thr Gly Gly Phe Thr Pro Ala Gly Ala
50 55 60
Thr Ser Asn Pro Ser Gly Phe Ser Thr Pro Lys Val Ala Glu Ser Pro
65 70 75 80
Ala Pro Gly Arg Ser Ser Gly Arg Lys Arg Ala Gly Gly Arg Leu Gln
85 90 95
Ser Ala Pro Thr Glu Trp Ile Leu Pro Gly Leu Val Leu Gly Leu Ile
100 105 110
Ser Leu Gly Leu Asn Ala Trp Ile Thr Phe Gly Ala Pro Val Ala Thr
115 120 125
Asp Asn Leu Phe Leu Ile Val Ala Gly Ile Ala Trp Val Leu Ala Gly
130 135 140
Ile Leu Ser Val Leu Ser Ile Gly Lys Tyr Met Ser Ala Val Asn Glu
145 150 155 160
Arg Lys Ser Thr Gly Phe Tyr Thr Glu Val Pro Trp Lys Lys Ala Leu
165 170 175
Phe Thr Ala Thr Ala Val Leu Leu Val Phe Ala Val Val Trp Ser Ala
180 185 190
Leu Asp Ile Ala Leu Trp Val Gly Lys Gln Ser Trp
195 200
<210>347
<211>1060
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1030)
<223>RXA03029
<400>347
aacacgcctg aggaacgccg tcccatcgcg gaggccgtga gcatgccgca cgaagaggtc 60
cgcgatttca tttacgcccg ccaaaactac ttcgatgccc ttg acc gcc gcg ccg 115
Leu Thr Ala Ala Pro
1 5
aag cca tgc gcc gcg caa ctg ggc tgg cag ccg tac gat tcc cgc gcc 163
Lys Pro Cys Ala Ala Gln Leu Gly Trp Gln Pro Tyr Asp Ser Arg Ala
10 15 20
atg gaa gat tcg atc gcc cgc cgc ctg caa atg gat cac gat gtc acc 211
Met Glu Asp Ser Ile Ala Arg Arg Leu Gln Met Asp His Asp Val Thr
25 30 35
atc acc tcc tcc aaa gag gaa tcc ggc acg ctg cac cac ttc gac ccc 259
Ile Thr Ser Ser Lys Glu Glu Ser Gly Thr Leu His His Phe Asp Pro
40 45 50
gag acg cgt ctg ctg aca atc cac gca cgc ctc aac ccc ggg caa cgc 307
Glu Thr Arg Leu Leu Thr Ile His Ala Arg Leu Asn Pro Gly Gln Arg
55 60 65
gcc ttc cgc atg gcc acc gaa ctc ggc tac cta gaa gcc aac gac ctc 355
Ala Phe Arg Met Ala Thr Glu Leu Gly Tyr Leu Glu Ala Asn Asp Leu
70 75 80 85
atc gaa ggt atc gtt gac gac ggc atc tgg tcc acc ccc gaa gcc cgc 403
Ile Glu Gly Ile Val Asp Asp Gly Ile Trp Ser Thr Pro Glu Ala Arg
90 95 100
acc cta gcc atc cgc ggt gtg gcc tcc tac ttc gcc gcc gcc gtg atg 451
Thr Leu Ala Ile Arg Gly Val Ala Ser Tyr Phe Ala Ala Ala Val Met
105 110 115
ctg ccc tac aaa atc ttc cac tcc gag gcc gaa aaa tcc ggc tac gac 499
Leu Pro Tyr Lys Ile Phe His Ser Glu Ala Glu Lys Ser Gly Tyr Asp
120 125 130
atc gag tac cta ggc caa ctc ttt ggc gtg ggc tat gag aca acc gcc 547
Ile Glu Tyr Leu Gly Gln Leu Phe Gly Val Gly Tyr Glu Thr Thr Ala
135 140 145
cac cgc ttg tcc acc ctg cag cgc ccc aac ctg cgc ggc atc ccc ttt 595
His Arg Leu Ser Thr Leu Gln Arg Pro Asn Leu Arg Gly Ile Pro Phe
150 155 160 165
acc ttc gtg cgc gtc gac cgc gcc ggc aac atg tcc aaa cgc caa tcc 643
Thr Phe Val Arg Val Asp Arg Ala Gly Asn Met Ser Lys Arg Gln Ser
170 175 180
gcc acc ggc ttc cac ttc acc cac tac ggc ggc acc tgc ccc ctg tgg 691
Ala Thr Gly Phe His Phe Thr His Tyr Gly Gly Thr Cys Pro Leu Trp
185 190 195
aac gtg ttt gaa acc ttc acc aac ccc ggc caa gtg ctc cgc caa ttc 739
Asn Val Phe Glu Thr Phe Thr Asn Pro Gly Gln Val Leu Arg Gln Phe
200 205 210
gcg caa atg ccc gac gga cgc aac tac ctg tgg atc tca cgc acc gtg 787
Ala Gln Met Pro Asp Gly Arg Asn Tyr Leu Trp Ile Ser Arg Thr Val
215 220 225
cga cac cac gaa gcc cgg ttc ggc gaa gta gac aaa atg ttc gcc atc 835
Arg His His Glu Ala Arg Phe Gly Glu Val Asp Lys Met Phe Ala Ile
230 235 240 245
ggc ctg ggc tgc gaa gcg cgc cac gcc gac cgc act gtg tac tcc cgc 883
Gly Leu Gly Cys Glu Ala Arg His Ala Asp Arg Thr Val Tyr Ser Arg
250 255 260
ggt ttc aac ctc cag gac ctc tcc acc gcc acc ccc atc ggg tcc ggc 931
Gly Phe Asn Leu Gln Asp Leu Ser Thr Ala Thr Pro Ile Gly Ser Gly
265 270 275
tgc cga gtg tgc acc cgc gag aac tgc gcg cag cgc gca ttc cca tcc 979
Cys Arg Val Cys Thr Arg Glu Asn Cys Ala Gln Arg Ala Phe Pro Ser
280 285 290
gtc cac ggc cgc atc aac atc gac gcg cac gaa tcc act atc gcg ccg 1027
Val His Gly Arg Ile Asn Ile Asp Ala His Glu Ser Thr Ile Ala Pro
295 300 305
tac taagaaaagg agcttgcttt acgacgcacc 1060
Tyr
310
<210>348
<211>310
<212>PRT
<213>谷氨酸棒状杆菌
<400>348
Leu Thr Ala Ala Pro Lys Pro Cys Ala Ala Gln Leu Gly Trp Gln Pro
1 5 10 15
Tyr Asp Ser Arg Ala Met Glu Asp Ser Ile Ala Arg Arg Leu Gln Met
20 25 30
Asp His Asp Val Thr Ile Thr Ser Ser Lys Glu Glu Ser Gly Thr Leu
35 40 45
His His Phe Asp Pro Glu Thr Arg Leu Leu Thr Ile His Ala Arg Leu
50 55 60
Asn Pro Gly Gln Arg Ala Phe Arg Met Ala Thr Glu Leu Gly Tyr Leu
65 70 75 80
Glu Ala Asn Asp Leu Ile Glu Gly Ile Val Asp Asp Gly Ile Trp Ser
85 90 95
Thr Pro Glu Ala Arg Thr Leu Ala Ile Arg Gly Val Ala Ser Tyr Phe
100 105 110
Ala Ala Ala Val Met Leu Pro Tyr Lys Ile Phe His Ser Glu Ala Glu
115 120 125
Lys Ser Gly Tyr Asp Ile Glu Tyr Leu Gly Gln Leu Phe Gly Val Gly
130 135 140
Tyr Glu Thr Thr Ala His Arg Leu Ser Thr Leu Gln Arg Pro Asn Leu
145 150 155 160
Arg Gly Ile Pro Phe Thr Phe Val Arg Val Asp Arg Ala Gly Asn Met
165 170 175
Ser Lys Arg Gln Ser Ala Thr Gly Phe His Phe Thr His Tyr Gly Gly
180 185 190
Thr Cys Pro Leu Trp Asn Val Phe Glu Thr Phe Thr Asn Pro Gly Gln
195 200 205
Val Leu Arg Gln Phe Ala Gln Met Pro Asp Gly Arg Asn Tyr Leu Trp
210 215 220
Ile Ser Arg Thr Val Arg His His Glu Ala Arg Phe Gly Glu Val Asp
225 230 235 240
Lys Met Phe Ala Ile Gly Leu Gly Cys Glu Ala Arg His Ala Asp Arg
245 250 255
Thr Val Tyr Ser Arg Gly Phe Asn Leu Gln Asp Leu Ser Thr Ala Thr
260 265 270
Pro Ile Gly Ser Gly Cys Arg Val Cys Thr Arg Glu Asn Cys Ala Gln
275 280 285
Arg Ala Phe Pro Ser Val His Gly Arg Ile Asn Ile Asp Ala His Glu
290 295 300
Ser Thr Ile Ala Pro Tyr
305 310
<210>349
<211>2032
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2002)
<223>RXA03053
<400>349
tattttgtcg tttttccaca atgaggatgc caaggatgaa ttcggtgctt tctggggcgt 60
caaccgcatt ccacgcaatc tgcctgaggg tgtctcagct gtg ctt ttt gag caa 115
Val Leu Phe Glu Gln
1 5
gtg gag cgg atg aag cag tca tgg att gat gac aat ctg aaa aat act 163
Val Glu Arg Met Lys Gln Ser Trp Ile Asp Asp Asn Leu Lys Asn Thr
l0 15 20
gac cga cta gct aag aca gaa gaa tca gac ttg gct gat tct tcc act 211
Asp Arg Leu Ala Lys Thr Glu Glu Ser Asp Leu Ala Asp Ser Ser Thr
25 30 35
aag tcc aac cgc cgc aag act gtt aag cgt gga ctt gat ctt gat gag 259
Lys Ser Asn Arg Arg Lys Thr Val Lys Arg Gly Leu Asp Leu Asp Glu
40 45 50
gtg atc gcc gag ctt gat gat ggt gca tca tat ttg act gtg cac aac 307
Val Ile Ala Glu Leu Asp Asp Gly Ala Ser Tyr Leu Thr Val His Asn
55 60 65
cgt ctg ctg ctg cgt gga cca aac ctt gag atc ttg gat tcg act gtt 355
Arg Leu Leu Leu Arg Gly Pro Asn Leu Glu Ile Leu Asp Ser Thr Val
70 75 80 85
gag aaa att cga cgc agt ttt atc gag cac tta tcg cga atg gat att 403
Glu Lys Ile Arg Arg Ser Phe Ile Glu His Leu Ser Arg Met Asp Ile
90 95 100
gct gca cac cac ggt gag caa cgt caa gaa ctg tca cag att ttt ggc 451
Ala Ala His His Gly Glu Gln Arg Gln Glu Leu Ser Gln Ile Phe Gly
105 110 115
aaa aat agc aaa aag cga ggt aaa gga ttc cac ttc acc tcg act gag 499
Lys Asn Ser Lys Lys Arg Gly Lys Gly Phe His Phe Thr Ser Thr Glu
120 125 130
ttt gcc ggt tct tat tct ttg gtc acc aat ggt ctt aac gat gtt gct 547
Phe Ala Gly Ser Tyr Ser Leu Val Thr Asn Gly Leu Asn Asp Val Ala
135 140 145
ggc gaa tac gtc ggt gtc atg cgt ggc gat gtc aac aac tcc gct gtg 595
Gly Glu Tyr Val Gly Val Met Arg Gly Asp Val Asn Asn Ser Ala Val
150 155 160 165
ctt ttt gat gtg gat cgc tgg tcg gga cat gtc gta tgc gca gat agc 643
Leu Phe Asp Val Asp Arg Trp Ser Gly His Val Val Cys Ala Asp Ser
170 175 180
tcg att aat aca tca cca cta ctt aag cgt ccc cgt gtc gtt gac atg 691
Ser Ile Asn Thr Ser Pro Leu Leu Lys Arg Pro Arg Val Val Asp Met
185 190 195
tgg ggg tcg aag att tct cag gca gcg cta ctg aat aac cgt cgc gta 739
Trp Gly Ser Lys Ile Ser Gln Ala Ala Leu Leu Asn Asn Arg Arg Val
200 205 210
gtg cac ctg gtg ctc aat ggt gcc aag ctc gat gaa atg gga cca aag 787
Val His Leu Val Leu Asn Gly Ala Lys Leu Asp Glu Met Gly Pro Lys
215 220 225
cta gaa tcc atg acc gct cat gtg gat atg act agc gga gat ctc aac 835
Leu Glu Ser Met Thr Ala His Val Asp Met Thr Ser Gly Asp Leu Asn
230 235 240 245
atg ttc gag ctg ttt ggt aag caa gaa gat gag ctg tcg ctg tat tcc 883
Met Phe Glu Leu Phe Gly Lys Gln Glu Asp Glu Leu Ser Leu Tyr Ser
250 255 260
aca cac atg gac aaa atc gtg ttt atg acc gag cag gca ttg ggc gat 931
Thr His Met Asp Lys Ile Val Phe Met Thr Glu Gln Ala Leu Gly Asp
265 270 275
aag acc tct gag ctg gcg cta ttg cgc gga aag ctc aaa gaa att att 979
Lys Thr Ser Glu Leu Ala Leu Leu Arg Gly Lys Leu Lys Glu Ile Ile
280 285 290
aca gcc ttt tat gtg gat atg aaa atg tgg gct ctt aat gcc ggt gaa 1027
Thr Ala Phe Tyr Val Asp Met Lys Met Trp Ala Leu Asn Ala Gly Glu
295 300 305
aac cgt gac aag ctg cgt ctt gtt ggt gtg cct cat gag cag atc ccg 1075
Asn Arg Asp Lys Leu Arg Leu Val Gly Val Pro His Glu Gln Ile Pro
310 315 320 325
ttg ctc tct gtc ttc gtg tcc tat ttg gat caa gaa tat gag cgc cag 1123
Leu Leu Ser Val Phe Val Ser Tyr Leu Asp Gln Glu Tyr Glu Arg Gln
330 335 340
aaa tat gag gga acc aaa gac cct gaa atg ttt cgt gcc att tct gtg 1171
Lys Tyr Glu Gly Thr Lys Asp Pro Glu Met Phe Arg Ala Ile Ser Val
345 350 355
ctg cga ctg acc tat aag gat ttg ctc gat act cat ggc gat ctg ttt 1219
Leu Arg Leu Thr Tyr Lys Asp Leu Leu Asp Thr His Gly Asp Leu Phe
360 365 370
aat cag cac acg gca gat ggc att gat tct gtg aac tct gca cgc cgc 1267
Asn Gln His Thr Ala Asp Gly Ile Asp Ser Val Asn Ser Ala Arg Arg
375 380 385
gtg atc tat gat ttt tct gga gtg ctg cga cgt ggc gca ggt gtc gcg 1315
Val Ile Tyr Asp Phe Ser Gly Val Leu Arg Arg Gly Ala Gly Val Ala
390 395 400 405
atg gca cag ctg gtt aac gtg att ggt ttt gct gtg gag acg ctt ggt 1363
Met Ala Gln Leu Val Asn Val Ile Gly Phe Ala Val Glu Thr Leu Gly
410 415 420
caa ggc gat gtg gtc atc att cac ggt gct gat ggc att gtc gat gtt 1411
Gln Gly Asp Val Val Ile Ile His Gly Ala Asp Gly Ile Val Asp Val
425 430 435
gat gtt cag gat tat ttg gct aat cag ttc gca tat atg gct gag cgt 1459
Asp Val Gln Asp Tyr Leu Ala Asn Gln Phe Ala Tyr Met Ala Glu Arg
440 445 450
ggt ggg cga gtg gcg tat ctc tac agc tcg atg gac gcg atg ctt ggc 1507
Gly Gly Arg Val Ala Tyr Leu Tyr Ser Ser Met Asp Ala Met Leu Gly
455 460 465
act gtt ggc ttc aat caa ttc caa cgt gct gcg tac acc att ttg ggc 1555
Thr Val Gly Phe Asn Gln Phe Gln Arg Ala Ala Tyr Thr Ile Leu Gly
470 475 480 485
ccg atg aac gtt gac tcg gtg gat act tat caa tct ctg att aat agt 1603
Pro Met Asn Val Asp Ser Val Asp Thr Tyr Gln Ser Leu Ile Asn Ser
490 495 500
cag atc ccg atg gac ttg gca cgg ttg gtg aca acg cag aat tct ggt 1651
Gln Ile Pro Met Asp Leu Ala Arg Leu Val Thr Thr Gln Asn Ser Gly
505 510 515
gcg agc tat ttg cgc cga gga tct acc aat gtg gtg ttt gaa acc aac 1699
Ala Ser Tyr Leu Arg Arg Gly Ser Thr Asn Val Val Phe Glu Thr Asn
520 525 530
ctt gcg ttg ggc gtt aat cct tat atg gag cag cgt cgc aag att gaa 1747
Leu Ala Leu Gly Val Asn Pro Tyr Met Glu Gln Arg Arg Lys Ile Glu
535 540 545
gca cag cga ggt cag cga cga tcc aag cga gac aag cac tac ggt ggt 1795
Ala Gln Arg Gly Gln Arg Arg Ser Lys Arg Asp Lys His Tyr Gly Gly
550 555 560 565
ggc acg acg atg gtg ggc aca gcg gat ctt gat gtt gtc gca att cag 1843
Gly Thr Thr Met Val Gly Thr Ala Asp Leu Asp Val Val Ala Ile Gln
570 575 580
gcg aag gcc gag cac cgc gaa gag cgc ttt gat gag aaa tct gcg cgc 1891
Ala Lys Ala Glu His Arg Glu Glu Arg Phe Asp Glu Lys Ser Ala Arg
585 590 595
aag atg aag gag ctt gat gat gta gaa gcg aaa gaa cta gct ttg aaa 1939
Lys Met Lys Glu Leu Asp Asp Val Glu Ala Lys Glu Leu Ala Leu Lys
600 605 610
act ggg ccg aag aat ctt gat gat gcg ctt gca cag gtg gag aag aag 1987
Thr Gly Pro Lys Asn Leu Asp Asp Ala Leu Ala Gln Val Glu Lys Lys
615 620 625
cgc ctg gcg aag cgg tagttgttgg ctgtggtgaa taaataagca 2032
Arg Leu Ala Lys Arg
630
<210>350
<211>634
<212>PRT
<213>谷氨酸棒状杆菌
<400>350
Val Leu Phe Glu Gln Val Glu Arg Met Lys Gln Ser Trp Ile Asp Asp
1 5 10 15
Asn Leu Lys Asn Thr Asp Arg Leu Ala Lys Thr Glu Glu Ser Asp Leu
20 25 30
Ala Asp Ser Ser Thr Lys Ser Asn Arg Arg Lys Thr Val Lys Arg Gly
35 40 45
Leu Asp Leu Asp Glu Val Ile Ala Glu Leu Asp Asp Gly Ala Ser Tyr
50 55 60
Leu Thr Val His Asn Arg Leu Leu Leu Arg Gly Pro Asn Leu Glu Ile
65 70 75 80
Leu Asp Ser Thr Val Glu Lys Ile Arg Arg Ser Phe Ile Glu His Leu
85 90 95
Ser Arg Met Asp Ile Ala Ala His His Gly Glu Gln Arg Gln Glu Leu
100 105 110
Ser Gln Ile Phe Gly Lys Asn Ser Lys Lys Arg Gly Lys Gly Phe His
115 120 125
Phe Thr Ser Thr Glu Phe Ala Gly Ser Tyr Ser Leu Val Thr Asn Gly
130 135 140
Leu Asn Asp Val Ala Gly Glu Tyr Val Gly Val Met Arg Gly Asp Val
145 150 155 160
Asn Asn Ser Ala Val Leu Phe Asp Val Asp Arg Trp Ser Gly His Val
165 170 175
Val Cys Ala Asp Ser Ser Ile Asn Thr Ser Pro Leu Leu Lys Arg Pro
180 185 190
Arg Val Val Asp Met Trp Gly Ser Lys Ile Ser Gln Ala Ala Leu Leu
195 200 205
Asn Asn Arg Arg Val Val His Leu Val Leu Asn Gly Ala Lys Leu Asp
210 215 220
Glu Met Gly Pro Lys Leu Glu Ser Met Thr Ala His Val Asp Met Thr
225 230 235 240
Ser Gly Asp Leu Asn Met Phe Glu Leu Phe Gly Lys Gln Glu Asp Glu
245 250 255
Leu Ser Leu Tyr Ser Thr His Met Asp Lys Ile Val Phe Met Thr Glu
260 265 270
Gln Ala Leu Gly Asp Lys Thr Ser Glu Leu Ala Leu Leu Arg Gly Lys
275 280 285
Leu Lys Glu Ile Ile Thr Ala Phe Tyr Val Asp Met Lys Met Trp Ala
290 295 300
Leu Asn Ala Gly Glu Asn Arg Asp Lys Leu Arg Leu Val Gly Val Pro
305 310 315 320
His Glu Gln Ile Pro Leu Leu Ser Val Phe Val Ser Tyr Leu Asp Gln
325 330 335
Glu Tyr Glu Arg Gln Lys Tyr Glu Gly Thr Lys Asp Pro Glu Met Phe
340 345 350
Arg Ala Ile Ser Val Leu Arg Leu Thr Tyr Lys Asp Leu Leu Asp Thr
355 360 365
His Gly Asp Leu Phe Asn Gln His Thr Ala Asp Gly Ile Asp Ser Val
370 375 380
Asn Ser Ala Arg Arg Val Ile Tyr Asp Phe Ser Gly Val Leu Arg Arg
385 390 395 400
Gly Ala Gly Val Ala Met Ala Gln Leu Val Asn Val Ile Gly Phe Ala
405 410 415
Val Glu Thr Leu Gly Gln Gly Asp Val Val Ile Ile His Gly Ala Asp
420 425 430
Gly Ile Val Asp Val Asp Val Gln Asp Tyr Leu Ala Asn Gln Phe Ala
435 440 445
Tyr Met Ala Glu Arg Gly Gly Arg Val Ala Tyr Leu Tyr Ser Ser Met
450 455 460
Asp Ala Met Leu Gly Thr Val Gly Phe Asn Gln Phe Gln Arg Ala Ala
465 470 475 480
Tyr Thr Ile Leu Gly Pro Met Asn Val Asp Ser Val Asp Thr Tyr Gln
485 490 495
Ser Leu Ile Asn Ser Gln Ile Pro Met Asp Leu Ala Arg Leu Val Thr
500 505 510
Thr Gln Asn Ser Gly Ala Ser Tyr Leu Arg Arg Gly Ser Thr Asn Val
515 520 525
Val Phe Glu Thr Asn Leu Ala Leu Gly Val Asn Pro Tyr Met Glu Gln
530 535 540
Arg Arg Lys Ile Glu Ala Gln Arg Gly Gln Arg Arg Ser Lys Arg Asp
545 550 555 560
Lys His Tyr Gly Gly Gly Thr Thr Met Val Gly Thr Ala Asp Leu Asp
565 570 575
Val Val Ala Ile Gln Ala Lys Ala Glu His Arg Glu Glu Arg Phe Asp
580 585 590
Glu Lys Ser Ala Arg Lys Met Lys Glu Leu Asp Asp Val Glu Ala Lys
595 600 605
Glu Leu Ala Leu Lys Thr Gly Pro Lys Asn Leu Asp Asp Ala Leu Ala
610 615 620
Gln Val Glu Lys Lys Arg Leu Ala Lys Arg
625 630
<210>351
<211>754
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(724)
<223>RXA03 098
<400>351
gaccgttttg tcgatcgcac cgctgctggc tcgcaccatc aacgagatct tcgaaaacgg 60
ttccgtcacc accctcttcg agggcgaggc ctaaacaccc atg ccc acc acg gac 115
Met Pro Thr Thr Asp
1 5
gtc ttc aac cgc gtc cgg ttg gca ttg gaa cct cta gct gat ccc gca 163
Val Phe Asn Arg Val Arg Leu Ala Leu Glu Pro Leu Ala Asp Pro Ala
10 15 20
cgt gcc acc gga atg gca agc tac atg cgg gat cag ttt tct ttt ctc 211
Arg Ala Thr Gly Met Ala Ser Tyr Met Arg Asp Gln Phe Ser Phe Leu
25 30 35
ggc atc cca tcc acc ccc aga aaa gaa gcc tgc aaa ccc gtg ctg tcc 259
Gly Ile Pro Ser Thr Pro Arg Lys Glu Ala Cys Lys Pro Val Leu Ser
40 45 50
gcg cta aaa gag ttg gac act gac ttt gtc tca gac tgc ttt ggc gca 307
Ala Leu Lys Glu Leu Asp Thr Asp Phe Val Ser Asp Cys Phe Gly Ala
55 60 65
gct gaa cgg gaa tac cag tat gtc gcc tgc gat cac atc aat cgc gtc 355
Ala Glu Arg Glu Tyr Gln Tyr Val Ala Cys Asp His Ile Asn Arg Val
70 75 80 85
ggc atc acc gat cta ggt ttt gcc aaa gca tta gtg cag acc aaa tcc 403
Gly Ile Thr Asp Leu Gly Phe Ala Lys Ala Leu Val Gln Thr Lys Ser
90 95 100
tgg tgg gac acc gtc gat tcc cta gca aaa ccg atc ggc gcc aaa cac 451
Trp Trp Asp Thr Val Asp Ser Leu Ala Lys Pro Ile Gly Ala Lys His
105 110 115
gat gat gat ctg atg aaa acg tgg gcg ctt gat gag gac ttc tgg gtg 499
Asp Asp Asp Leu Met Lys Thr Trp Ala Leu Asp Glu Asp Phe Trp Val
120 125 130
cgc cgc atc gcg atc atc cac caa ctg ggc cgc aag aaa aac acc gac 547
Arg Arg Ile Ala Ile Ile His Gln Leu Gly Arg Lys Lys Asn Thr Asp
135 140 145
gct gcc ctg ctg gcc tgg atc atc gag cag aac ctc ggc tcc agc gag 595
Ala Ala Leu Leu Ala Trp Ile Ile Glu Gln Asn Leu Gly Ser Ser Glu
150 155 160 165
ttc ttc atc aac aaa gcg atc ggc tgg gca ctg cgg gat ttc gcc cgc 643
Phe Phe Ile Asn Lys Ala Ile Gly Trp Ala Leu Arg Asp Phe Ala Arg
170 175 180
cac gac ccc agc tgg gtc cgg gct ttt gtc gac gcc acg gac ctt tcc 691
His Asp Pro Ser Trp Val Arg Ala Phe Val Asp Ala Thr Asp Leu Ser
185 190 195
cca ctg agc cgg cga gaa gcc ctg aag aat att tagccctcag gcatcatctg 744
Pro Leu Ser Arg Arg Glu Ala Leu Lys Asn Ile
200 205
agcgagtgcc 754
<210>352
<211>208
<212>PRT
<213>谷氨酸棒状杆菌
<400>352
Met Pro Thr Thr Asp Val Phe Asn Arg Val Arg Leu Ala Leu Glu Pro
1 5 10 15
Leu Ala Asp Pro Ala Arg Ala Thr Gly Met Ala Ser Tyr Met Arg Asp
20 25 30
Gln Phe Ser Phe Leu Gly Ile Pro Ser Thr Pro Arg Lys Glu Ala Cys
35 40 45
Lys Pro Val Leu Ser Ala Leu Lys Glu Leu Asp Thr Asp Phe Val Ser
50 55 60
Asp Cys Phe Gly Ala Ala Glu Arg Glu Tyr Gln Tyr Val Ala Cys Asp
65 70 75 80
His Ile Asn Arg Val Gly Ile Thr Asp Leu Gly Phe Ala Lys Ala Leu
85 90 95
Val Gln Thr Lys Ser Trp Trp Asp Thr Val Asp Ser Leu Ala Lys Pro
100 105 110
Ile Gly Ala Lys His Asp Asp Asp Leu Met Lys Thr Trp Ala Leu Asp
115 120 125
Glu Asp Phe Trp Val Arg Arg Ile Ala Ile Ile His Gln Leu Gly Arg
130 135 140
Lys Lys Asn Thr Asp Ala Ala Leu Leu Ala Trp Ile Ile Glu Gln Asn
145 150 155 160
Leu Gly Ser Ser Glu Phe Phe Ile Asn Lys Ala Ile Gly Trp Ala Leu
165 170 175
Arg Asp Phe Ala Arg His Asp Pro Ser Trp Val Arg Ala Phe Val Asp
180 185 190
Ala Thr Asp Leu Ser Pro Leu Ser Arg Arg Glu Ala Leu Lys Asn Ile
195 200 205
<210>353
<211>1729
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1699)
<223>RXA03113
<400>353
gttggatctt gcattgaaaa aatgaaaacc tcgtcgggaa tgcaacttgg gatcacgtct 60
cgggcaagaa acgtccttaa aaaaagggga gtgattgtga gtg ctt gat ttc tta 115
Val Leu Asp Phe Leu
1 5
gct gcg aac ccg ctg att gcg ctg gtg gtt att ttg gcc gtt ggt tta 163
Ala Ala Asn Pro Leu Ile Ala Leu Val Val Ile Leu Ala Val Gly Leu
10 15 20
gca att ggt cag att agg gtc ttt ggc ctt tct tta ggt gcc gcc gcg 211
Ala Ile Gly Gln Ile Arg Val Phe Gly Leu Ser Leu Gly Ala Ala Ala
25 30 35
gtg ctg ttt gtg gcc ctg gtg gtt tca act gca aat acc gac atc gtc 259
Val Leu Phe Val Ala Leu Val Val Ser Thr Ala Asn Thr Asp Ile Val
40 45 50
atc ccc atg att gtt tat cag ctg ggc ttg gcg atg ttc gtt tat gtc 307
Ile Pro Met Ile Val Tyr Gln Leu Gly Leu Ala Met Phe Val Tyr Val
55 60 65
atc ggt ttg tcc gcc gga cca gca ttt ttc agt gag ttc gct aaa aag 355
Ile Gly Leu Ser Ala Gly Pro Ala Phe Phe Ser Glu Phe Ala Lys Lys
70 75 80 85
ggc tgg aag ctc acc atc ttt atg ctc ctg ctg ctg gca aca ctg att 403
Gly Trp Lys Leu Thr Ile Phe Met Leu Leu Leu Leu Ala Thr Leu Ile
90 95 100
ggt ttg gcg tgg gtg ctt att aag tca ctg ggg ctt gat gca gcg atc 451
Gly Leu Ala Trp Val Leu Ile Lys Ser Leu Gly Leu Asp Ala Ala Ile
105 110 115
ggt acc ggt atg ttc acc ggc gcg ctg acc tcg act ccc ggt atg gca 499
Gly Thr Gly Met Phe Thr Gly Ala Leu Thr Ser Thr Pro Gly Met Ala
120 125 130
gcg gtc gtg gaa ttg att gaa gga atc gat cca agc ctt gcc agt gaa 547
Ala Val Val Glu Leu Ile Glu Gly Ile Asp Pro Ser Leu Ala Ser Glu
135 140 145
cct gtt att ggt tat tcc ttg gca tat ccg gga gcc gtg ctg gga tcc 595
Pro Val Ile Gly Tyr Ser Leu Ala Tyr Pro Gly Ala Val Leu Gly Ser
150 155 160 165
att gtg gtg gcc gcg gtt gga gcg aaa ctg ctc aaa gta aat cac cgg 643
Ile Val Val Ala Ala Val Gly Ala Lys Leu Leu Lys Val Asn His Arg
170 175 180
gaa gat gct cga aaa gaa ggc atg atc acc gca ccg ctg gtg tgg aag 691
Glu Asp Ala Arg Lys Glu Gly Met Ile Thr Ala Pro Leu Val Trp Lys
185 190 195
ggt gtg cag ctc aaa cct gga atc aca ggc agg gtg gga gat ctt cca 739
Gly Val Gln Leu Lys Pro Gly Ile Thr Gly Arg Val Gly Asp Leu Pro
200 205 210
cgc ctt gca ggt gaa agt atc atc gca acc cgc att gtg gat gat cca 787
Arg Leu Ala Gly Glu Ser Ile Ile Ala Thr Arg Ile Val Asp Asp Pro
215 220 225
cat aca cac cgc ctc gcg gat cca gat ctg ccg att act gaa ggc atg 835
His Thr His Arg Leu Ala Asp Pro Asp Leu Pro Ile Thr Glu Gly Met
230 235 240 245
gaa ctg ttg atc aac ggc act gaa gaa gcc gtg gat cgg gca att aag 883
Glu Leu Leu Ile Asn Gly Thr Glu Glu Ala Val Asp Arg Ala Ile Lys
250 255 260
gcg ttg ggt gaa gaa cgc gaa acc aaa att gag gac aca gag ctg atc 931
Ala Leu Gly Glu Glu Arg Glu Thr Lys Ile Glu Asp Thr Glu Leu Ile
265 270 275
tac acc cgc ctg acg gta tct agc cct gag gtt gca ggt aga acc gtt 979
Tyr Thr Arg Leu Thr Val Ser Ser Pro Glu Val Ala Gly Arg Thr Val
280 285 290
gct gag ctt gat act gta gct cac gga ttc atg att gcc cgt atc cgc 1027
Ala Glu Leu Asp Thr Val Ala His Gly Phe Met Ile Ala Arg Ile Arg
295 300 305
cag ggc gat tct gag gta gtg cct aaa cct gac acc gtg atc aac tac 1075
Gln Gly Asp Ser Glu Val Val Pro Lys Pro Asp Thr Val Ile Asn Tyr
310 315 320 325
tct gac cgc atc cgc gtg gtg gtt gct cct ggt cgt gtg gct gaa gtg 1123
Ser Asp Arg Ile Arg Val Val Val Ala Pro Gly Arg Val Ala Glu Val
330 335 340
cga cga ttc tta ggg gac tct gaa aag tcc ctt gct gat gtt aat ctg 1171
Arg Arg Phe Leu Gly Asp Ser Glu Lys Ser Leu Ala Asp Val Asn Leu
345 350 355
ctg cct tta gcc atc gga tta tct ctt ggc ctg ttg ttg ggc gcg atc 1219
Leu Pro Leu Ala Ile Gly Leu Ser Leu Gly Leu Leu Leu Gly Ala Ile
360 365 370
ccg att cct ctt cca ggc ggc acc acg atg tcc ctt ggc ttt ggt ggc 1267
Pro Ile Pro Leu Pro Gly Gly Thr Thr Met Ser Leu Gly Phe Gly Gly
375 380 385
ggc ccg att att gcc ggc ctg att ttg gga gca ctc aag cac aca gga 1315
Gly Pro Ile Ile Ala Gly Leu Ile Leu Gly Ala Leu Lys His Thr Gly
390 395 400 405
ccg ctg acg tgg cag atg ccg ttc cac gcc aac cgc acg atc tcc acc 1363
Pro Leu Thr Trp Gln Met Pro Phe His Ala Asn Arg Thr Ile Ser Thr
410 415 420
ttg ggc ctg gcg ctg ttt ttg gct ggt gtg ggt acc tct gca ggt gca 1411
Leu Gly Leu Ala Leu Phe Leu Ala Gly Val Gly Thr Ser Ala Gly Ala
425 430 435
gga ttt aga gct gcg ctt acc gat tcc tcc tcc ttg atc tac atg gcc 1459
Gly Phe Arg Ala Ala Leu Thr Asp Ser Ser Ser Leu Ile Tyr Met Ala
440 445 450
ggt ggc ttg gtg atc acc ttg gcc tct gcg ctg ctg tgt gct gtg atc 1507
Gly Gly Leu Val Ile Thr Leu Ala Ser Ala Leu Leu Cys Ala Val Ile
455 460 465
ggc atg tgg gta ctc agg ttg agg tgg gat gaa gcc atg ggt gtt gcc 1555
Gly Met Trp Val Leu Arg Leu Arg Trp Asp Glu Ala Met Gly Val Ala
470 475 480 485
gct ggc acc acc aca aat cct gca att att tcc tat ctg aat ggg caa 1603
Ala Gly Thr Thr Thr Asn Pro Ala Ile Ile Ser Tyr Leu Asn Gly Gln
490 495 500
acc gga acg gat ctt gcc aac agg gga tat gcc act gtg tac ccc acg 1651
Thr Gly Thr Asp Leu Ala Asn Arg Gly Tyr Ala Thr Val Tyr Pro Thr
505 510 515
gcg atg atc ggt aaa atc ctc ggc gcg cag ata ttg ttc ttg ctg ctc 1699
Ala Met Ile Gly Lys Ile Leu Gly Ala Gln Ile Leu Phe Leu Leu Leu
520 525 530
taaggtgatt tttgggcagt ggtgggggtg 1729
<210>354
<211>533
<212>PRT
<213>谷氨酸棒状杆菌
<400>354
Val Leu Asp Phe Leu Ala Ala Asn Pro Leu Ile Ala Leu Val Val Ile
1 5 10 15
Leu Ala Val Gly Leu Ala Ile Gly Gln Ile Arg Val Phe Gly Leu Ser
20 25 30
Leu Gly Ala Ala Ala Val Leu Phe Val Ala Leu Val Val Ser Thr Ala
35 40 45
Asn Thr Asp Ile Val Ile Pro Met Ile Val Tyr Gln Leu Gly Leu Ala
50 55 60
Met Phe Val Tyr Val Ile Gly Leu Ser Ala Gly Pro Ala Phe Phe Ser
65 70 75 80
Glu Phe Ala Lys Lys Gly Trp Lys Leu Thr Ile Phe Met Leu Leu Leu
85 90 95
Leu Ala Thr Leu Ile Gly Leu Ala Trp Val Leu Ile Lys Ser Leu Gly
100 105 110
Leu Asp Ala Ala Ile Gly Thr Gly Met Phe Thr Gly Ala Leu Thr Ser
115 120 125
Thr Pro Gly Met Ala Ala Val Val Glu Leu Ile Glu Gly Ile Asp Pro
130 135 140
Ser Leu Ala Ser Glu Pro Val Ile Gly Tyr Ser Leu Ala Tyr Pro Gly
145 150 155 160
Ala Val Leu Gly Ser Ile Val Val Ala Ala Val Gly Ala Lys Leu Leu
165 170 175
Lys Val Asn His Arg Glu Asp Ala Arg Lys Glu Gly Met Ile Thr Ala
180 185 190
Pro Leu Val Trp Lys Gly Val Gln Leu Lys Pro Gly Ile Thr Gly Arg
195 200 205
Val Gly Asp Leu Pro Arg Leu Ala Gly Glu Ser Ile Ile Ala Thr Arg
210 215 220
Ile Val Asp Asp Pro His Thr His Arg Leu Ala Asp Pro Asp Leu Pro
225 230 235 240
Ile Thr Glu Gly Met Glu Leu Leu Ile Asn Gly Thr Glu Glu Ala Val
245 250 255
Asp Arg Ala Ile Lys Ala Leu Gly Glu Glu Arg Glu Thr Lys Ile Glu
260 265 270
Asp Thr Glu Leu Ile Tyr Thr Arg Leu Thr Val Ser Ser Pro Glu Val
275 280 285
Ala Gly Arg Thr Val Ala Glu Leu Asp Thr Val Ala His Gly Phe Met
290 295 300
Ile Ala Arg Ile Arg Gln Gly Asp Ser Glu Val Val Pro Lys Pro Asp
305 310 315 320
Thr Val Ile Asn Tyr Ser Asp Arg Ile Arg Val Val Val Ala Pro Gly
325 330 335
Arg Val Ala Glu Val Arg Arg Phe Leu Gly Asp Ser Glu Lys Ser Leu
340 345 350
Ala Asp Val Asn Leu Leu Pro Leu Ala Ile Gly Leu Ser Leu Gly Leu
355 360 365
Leu Leu Gly Ala Ile Pro Ile Pro Leu Pro Gly Gly Thr Thr Met Ser
370 375 380
Leu Gly Phe Gly Gly Gly Pro Ile Ile Ala Gly Leu Ile Leu Gly Ala
385 390 395 400
Leu Lys His Thr Gly Pro Leu Thr Trp Gln Met Pro Phe His Ala Asn
405 410 415
Arg Thr Ile Ser Thr Leu Gly Leu Ala Leu Phe Leu Ala Gly Val Gly
420 425 430
Thr Ser Ala Gly Ala Gly Phe Arg Ala Ala Leu Thr Asp Ser Ser Ser
435 440 445
Leu Ile Tyr Met Ala Gly Gly Leu Val Ile Thr Leu Ala Ser Ala Leu
450 455 460
Leu Cys Ala Val Ile Gly Met Trp Val Leu Arg Leu Arg Trp Asp Glu
465 470 475 480
Ala Met Gly Val Ala Ala Gly Thr Thr Thr Asn Pro Ala Ile Ile Ser
485 490 495
Tyr Leu Asn Gly Gln Thr Gly Thr Asp Leu Ala Asn Arg Gly Tyr Ala
500 505 510
Thr Val Tyr Pro Thr Ala Met Ile Gly Lys Ile Leu Gly Ala Gln Ile
515 520 525
Leu Phe Leu Leu Leu
530
<210>355
<211>811
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(781)
<223>RXA03184
<400>355
taccaaaaca atctgccgat taagtaggaa gtccccaatg accgccgttt tgctcctcac 60
tctttttcta tctcttttta ttggcatttc gctaggactg ttg ggc ggt ggt ggt 115
Leu Gly Gly Gly Gly
1 5
tcg atc ctc act gtt cca att ctg acg tat gtg gcg ggg ttg gag cct 163
Ser Ile Leu Thr Val Pro Ile Leu Thr Tyr Val Ala Gly Leu Glu Pro
10 15 20
cgt gaa gct att gcc gcc tcg ctg ttt att gtg ggc aca acg tcg ctg 211
Arg Glu Ala Ile Ala Ala Ser Leu Phe Ile Val Gly Thr Thr Ser Leu
25 30 35
gtc agtgcc att agt cac gca aga aat ggg cgg gtc cgg tgg aag act 259
Val Ser Ala Ile Ser His Ala Arg Asn Gly Arg Val Arg Trp Lys Thr
40 45 50
ggc ctg ctg ttt ggt gcc gcg ggc atg gtt ggc gcg ttt ggc ggt ggc 307
Gly Leu Leu Phe Gly Ala Ala Gly Met Val Gly Ala Phe Gly Gly Gly
55 60 65
gtt ctc ggg ggc tac att cct ggc acg att ttg atg atc gcg ttt gcg 355
Val Leu Gly Gly Tyr Ile Pro Gly Thr Ile Leu Met Ile Ala Phe Ala
70 75 80 85
ctc atg atg atc gcc acg tcg act gcg atg ttg cgg ggg cgt aag cag 403
Leu Met Met Ile Ala Thr Ser Thr Ala Met Leu Arg Gly Arg Lys Gln
90 95 100
aag aag ggg gcg tcg aaa agc tct ctt tgg cgc gtc ctt gtt gat ggc 451
Lys Lys Gly Ala Ser Lys Ser Ser Leu Trp Arg Val Leu Val Asp Gly
105 110 115
ctg gtg gtc ggc gcg gtg acc ggg ctt gtt ggt gcg ggc ggc ggc ttt 499
Leu Val Val Gly Ala Val Thr Gly Leu Val Gly Ala Gly Gly Gly Phe
120 125 130
ttg gtg gtg ccg gcg ctg gcg ctg ctc ggc ggg ctg tcg atg ccg gtg 547
Leu Val Val Pro Ala Leu Ala Leu Leu Gly Gly Leu Ser Met Pro Val
135 140 145
gct gtg ggc acg tcg ttg gtg gtg atc acg atg aag tcg ttt gcg ggg 595
Ala Val Gly Thr Ser Leu Val Val Ile Thr Met Lys Ser Phe Ala Gly
150 155 160 165
ctt gcc ggg tat ctg acc agc gtg cag ctg gat tgg ggg ctg gtg ctg 643
Leu Ala Gly Tyr Leu Thr Ser Val Gln Leu Asp Trp Gly Leu Val Leu
170 175 180
atg gtg act gcg gcc gcc atc gtc ggt tcg ctt gcc ggt tcg cgc ctt 691
Met Val Thr Ala Ala Ala Ile Val Gly Ser Leu Ala Gly Ser Arg Leu
185 190 195
gcg ggg cgc gtg cct gag act ttg ctc cgc aaa ggg ttc ggg gtg ttc 739
Ala Gly Arg Val Pro Glu Thr Leu Leu Arg Lys Gly Phe Gly Val Phe
200 205 210
gtg ctg gtc atg ggc gtg ttc gtg ctc ggc ttg gag ctt tta 781
Val Leu Val Met Gly Val Phe Val Leu GlV Leu Glu Leu Leu
215 220 225
taagcttttc gacgtctccc tccggcgaaa 811
<210>356
<211>227
<212>PRT
<213>谷氨酸棒状杆菌
<400>356
Leu Gly Gly Gly Gly Ser Ile Leu Thr Val Pro Ile Leu Thr Tyr Val
1 5 10 15
Ala Gly Leu Glu Pro Arg Glu Ala Ile Ala Ala Ser Leu Phe Ile Val
20 25 30
Gly Thr Thr Ser Leu Val Ser Ala Ile Ser His Ala Arg Asn Gly Arg
35 40 45
Val Arg Trp Lys Thr Gly Leu Leu Phe Gly Ala Ala Gly Met Val Gly
50 55 60
Ala Phe Gly Gly Gly Val Leu Gly Gly Tyr Ile Pro Gly Thr Ile Leu
65 70 75 80
Met Ile Ala Phe Ala Leu Met Met Ile Ala Thr Ser Thr Ala Met Leu
85 90 95
Arg Gly Arg Lys Gln Lys Lys Gly Ala Ser Lys Ser Ser Leu Trp Arg
100 105 110
Val Leu Val Asp Gly Leu Val Val Gly Ala Val Thr Gly Leu Val Gly
115 120 125
Ala Gly Gly Gly Phe Leu Val Val Pro Ala Leu Ala Leu Leu Gly Gly
130 135 140
Leu Ser Met Pro Val Ala Val Gly Thr Ser Leu Val Val Ile Thr Met
145 150 155 160
Lys Ser Phe Ala Gly Leu Ala Gly Tyr Leu Thr Ser Val Gln Leu Asp
165 170 175
Trp Gly Leu Val Leu Met Val Thr Ala Ala Ala Ile Val Gly Ser Leu
180 185 190
Ala Gly Ser Arg Leu Ala Gly Arg Val Pro Glu Thr Leu Leu Arg Lys
195 200 205
Gly Phe Gly Val Phe Val Leu Val Met Gly Val Phe Val Leu Gly Leu
210 215 220
Glu Leu Leu
225
<210>357
<211>601
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(571)
<223>RXA03316
<400>357
tctgcgttaa gttcgacttc ttaacaccga cttcagtgag aaaacctgac atcgccgtat 60
ttcccaaacg caactgtgtg ctgagataag atcagtaatc atg act tac aaa gca 115
Met Thr Tyr Lys Ala
1 5
gac tat gat ctt gac ttt gaa aaa gta gtc ttc cgc atg tca cag ctg 163
Asp Tyr Asp Leu Asp Phe Glu Lys Val Val Phe Arg Met Ser Gln Leu
10 15 20
ttt gaa gat ctc atc ccg tca gaa aac gcc tac gac cca tac aaa aaa 211
Phe Glu Asp Leu Ile Pro Ser Glu Asn Ala Tyr Asp Pro Tyr Lys Lys
25 30 35
tca acg tgg ccg act ctg acc atg gct gca aca tgg gta cag gac gac 259
Ser Thr Trp Pro Thr Leu Thr Met Ala Ala Thr Trp Val Gln Asp Asp
40 45 50
tgt ctc ata gtt gta ttt agg gct gat cca aga aga gtt gcg cca atg 307
Cys Leu Ile Val Val Phe Arg Ala Asp Pro Arg Arg Val Ala Pro Met
55 60 65
gat ggt gcg act ttc gtg ggc tac ttt tgc aag att tac ccc caa tac 355
Asp Gly Ala Thr Phe Val Gly Tyr Phe Cys Lys Ile Tyr Pro Gln Tyr
70 75 80 85
att cct gac aat atc gaa ttt ggg gca gta gag cta atg tat gcc gac 403
Ile Pro Asp Asn Ile Glu Phe Gly Ala Val Glu Leu Met Tyr Ala Asp
90 95 100
tgg atg gca ggt gat ggg ttt att tgg aag atc gat ctc aag gct ccg 451
Trp Met Ala Gly Asp Gly Phe Ile Trp Lys Ile Asp Leu Lys Ala Pro
105 110 115
caa aag ata cat tgg aga aat gca ttc gat ggt cta aaa gct ccg gca 499
Gln Lys Ile His Trp Arg Asn Ala Phe Asp Gly Leu Lys Ala Pro Ala
120 125 130
gat tta gag gaa tta ttg gac tac gcc gcg gag att ccg atg tgg tta 547
Asp Leu Glu Glu Leu Leu Asp Tyr Ala Ala Glu Ile Pro Met Trp Leu
135 140 145
aac ccg gaa att gaa aag tgg atg tagccagctt caaatttgaa taccccttga 601
Asn Pro Glu Ile Glu Lys Trp Met
150 155
<210>358
<211>157
<212>PRT
<213>谷氨酸棒状杆菌
<400>358
Met Thr Tyr Lys Ala Asp Tyr Asp Leu Asp Phe Glu Lys Val Val Phe
1 5 10 15
Arg Met Ser Gln Leu Phe Glu Asp Leu Ile Pro Ser Glu Asn Ala Tyr
20 25 30
Asp Pro Tyr Lys Lys Ser Thr Trp Pro Thr Leu Thr Met Ala Ala Thr
35 40 45
Trp Val Gln Asp Asp Cys Leu Ile Val Val Phe Arg Ala Asp Pro Arg
50 55 60
Arg Val Ala Pro Met Asp Gly Ala Thr Phe Val Gly Tyr Phe Cys Lys
65 70 75 80
Ile Tyr Pro Gln Tyr Ile Pro Asp Asn Ile Glu Phe Gly Ala Val Glu
85 90 95
Leu Met Tyr Ala Asp Trp Met Ala Gly Asp Gly Phe Ile Trp Lys Ile
100 105 110
Asp Leu Lys Ala Pro Gln Lys Ile His Trp Arg Asn Ala Phe Asp Gly
115 120 125
Leu Lys Ala Pro Ala Asp Leu Glu Glu Leu Leu Asp Tyr Ala Ala Glu
130 135 140
Ile Pro Met Trp Leu Asn Pro Glu Ile Glu Lys Trp Met
145 150 155
<2l0>359
<211>1213
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1183)
<223>RXA03343
<400>359
ccgcgctctg atctggtact tctgagatag tcagcaaatc tgatatagga aaactcctta 60
tcagaggaag tatagattgt gaattttgaa ggattacgcg gtg cat act cgt ggg 115
Val His Thr Arg Gly
1 5
tta gtt tct ctc tta agt ctt att ttt ctt ttc gtt ctt tca ggg tgt 163
Leu Val Ser Leu Leu Ser Leu Ile Phe Leu Phe Val Leu Ser Gly Cys
10 15 20
gga ggg aat gcc act acc gct gat gaa gct gct gaa agt gac gtt gtt 211
Gly Gly Asn Ala Thr Thr Ala Asp Glu Ala Ala Glu Ser Asp Val Val
25 30 35
act tca agt agc gca cct cca agt aaa cga gct cta gat gtt gga gaa 259
Thr Ser Ser Ser Ala Pro Pro Ser Lys Arg Ala Leu Asp Val Gly Glu
40 45 50
gca gta gag atc cca ggg gtc gtg ctc acg gta aac agc gtc aca cag 307
Ala Val Glu Ile Pro Gly Val Val Leu Thr Val Asn Ser Val Thr Gln
55 60 65
tcc gat cag ctc atg ttg tac tca gaa ggc agt gcg cgt gga tct gag 355
Ser Asp Gln Leu Met Leu Tyr Ser Glu Gly Ser Ala Arg Gly Ser Glu
70 75 80 85
ccg aga gaa cag cgg aat gct gcg tcc gga gaa aag ttt gtt tcg gtg 403
Pro Arg Glu Gln Arg Asn Ala Ala Ser Gly Glu Lys Phe Val Ser Val
90 95 100
gac act acc gtg aag aat tcg agc tct gat cct tgg gat ctt tca tgt 451
Asp Thr Thr Val Lys Asn Ser Ser Ser Asp Pro Trp Asp Leu Ser Cys
105 110 115
ggt cac gta tta cag aca tgg ctc ctc gag gac gaa cta gat gat caa 499
Gly His Val Leu Gln Thr Trp Leu Leu Glu Asp Glu Leu Asp Asp Gln
120 125 130
caa ggt gac caa gaa aag aaa ttt agc cct atc gac aat ctt gat cag 547
Gln Gly Asp Gln Glu Lys Lys Phe Ser Pro Ile Asp Asn Leu Asp Gln
135 140 145
att tct ggt aat ccg gag tgt ggt gtg ctt tta gaa gtg ggt aca gaa 595
Ile Ser Gly Asn Pro Glu Cys Gly Val Leu Leu Glu Val Gly Thr Glu
150 155 160 165
att gaa atg acg tgg tct ttt act att cca gat gac ata gaa atc act 643
Ile Glu Met Thr Trp Ser Phe Thr Ile Pro Asp Asp Ile Glu Ile Thr
170 175 180
cac ttt ggt ttt tct ctt tcg gat agc act tcc aat gac ttg gcc att 691
His Phe Gly Phe Ser Leu Ser Asp Ser Thr Ser Asn Asp Leu Ala Ile
185 190 195
atc agc ctt ggg ggt gca ata gag aca tcg tcg gcc atc aca aca act 739
Ile Ser Leu Gly Gly Ala Ile Glu Thr Ser Ser Ala Ile Thr Thr Thr
200 205 210
gaa gtt ata gca ccg gaa aat gac acg gaa acg ctg cta gag ata act 787
Glu Val Ile Ala Pro Glu Asn Asp Thr Glu Thr Leu Leu Glu Ile Thr
215 220 225
ccg gtt gac tgt cag gtc ggc ctt ggc ccc atc gtt act tct tgg tcg 835
Pro Val Asp Cys Gln Val Gly Leu Gly Pro Ile Val Thr Ser Trp Ser
230 235 240 245
gat gga acg gtc gga ggt tgg tct cag cac tgt cag gac gtg cat gat 883
Asp Gly Thr Val Gly Gly Trp Ser Gln His Cys Gln Asp Val His Asp
250 255 260
gag gta ctt gcc ggt gaa gtc gca gcg aat acc cca gta tgt gac gga 931
Glu Val Leu Ala Gly Glu Val Ala Ala Asn Thr Pro Val Cys Asp Gly
265 270 275
gtt gta tgc act tac ccc agt ggc gcc aca atg ccg gat cca aat gca 979
Val Val Cys Thr Tyr Pro Ser Gly Ala Thr Met Pro Asp Pro Asn Ala
280 285 290
cct caa atc cct tca gat acg agt ggt gca gtg tgc gat gaa aat cag 1027
Pro Gln Ile Pro Ser Asp Thr Ser Gly Ala Val Cys Asp Glu Asn Gln
295 300 305
tgt gtt tac cct aac ggt tat atc gcc cga att ggg gat ccg aat gtt 1075
Cys Val Tyr Pro Asn Gly Tyr Ile Ala Arg Ile Gly Asp Pro Asn Val
310 315 320 325
ccg aac tat ctc aag cct ggc aac tct ccg tgg gta caa gga cag att 1123
Pro Asn Tyr Leu Lys Pro Gly Asn Ser Pro Trp Val Gln Gly Gln Ile
330 335 340
gat ttt cag aat tgc cta gac agt ggg aaa act att gag caa tgc cgt 1171
Asp Phe Gln Asn Cys Leu Asp Ser Gly Lys Thr Ile Glu Gln Cys Arg
345 350 355
gaa gaa ctg aac taggtcagtc ttaagaaatc acccagcccg 1213
Glu Glu Leu Asn
360
<210>360
<211>361
<212>PRT
<213>谷氨酸棒状杆菌
<400>360
Val His Thr Arg Gly Leu Val Ser Leu Leu Ser Leu Ile Phe Leu Phe
1 5 10 15
Val Leu Ser Gly Cys Gly Gly Asn Ala Thr Thr Ala Asp Glu Ala Ala
20 25 30
Glu Ser Asp Val Val Thr Ser Ser Ser Ala Pro Pro Ser Lys Arg Ala
35 40 45
Leu Asp Val Gly Glu Ala Val Glu Ile Pro Gly Val Val Leu Thr Val
50 55 60
Asn Ser Val Thr Gln Ser Asp Gln Leu Met Leu Tyr Ser Glu Gly Ser
65 70 75 80
Ala Arg Gly Ser Glu Pro Arg Glu Gln Arg Asn Ala Ala Ser Gly Glu
85 90 95
Lys Phe Val Ser Val Asp Thr Thr Val Lys Asn Ser Ser Ser Asp Pro
100 105 110
Trp Asp Leu Ser Cys Gly His Val Leu Gln Thr Trp Leu Leu Glu Asp
115 120 125
Glu Leu Asp Asp Gln Gln Gly Asp Gln Glu Lys Lys Phe Ser Pro Ile
130 135 140
Asp Asn Leu Asp Gln Ile Ser Gly Asn Pro Glu Cys Gly Val Leu Leu
145 150 155 160
Glu Val Gly Thr Glu Ile Glu Met Thr Trp Ser Phe Thr Ile Pro Asp
165 170 175
Asp Ile Glu Ile Thr His Phe Gly Phe Ser Leu Ser Asp Ser Thr Ser
180 185 190
Asn Asp Leu Ala Ile Ile Ser Leu Gly Gly Ala Ile Glu Thr Ser Ser
195 200 205
Ala Ile Thr Thr Thr Glu Val Ile Ala Pro Glu Asn Asp Thr Glu Thr
210 215 220
Leu Leu Glu Ile Thr Pro Val Asp Cys Gln Val Gly Leu Gly Pro Ile
225 230 235 240
Val Thr Ser Trp Ser Asp Gly Thr Val Gly Gly Trp Ser Gln His Cys
245 250 255
Gln Asp Val His Asp Glu Val Leu Ala Gly Glu Val Ala Ala Asn Thr
260 265 270
Pro Val Cys Asp Gly Val Val Cys Thr Tyr Pro Ser Gly Ala Thr Met
275 280 285
Pro Asp Pro Asn Ala Pro Gln Ile Pro Ser Asp Thr Ser Gly Ala Val
290 295 300
Cys Asp Glu Asn Gln Cys Val Tyr Pro Asn Gly Tyr Ile Ala Arg Ile
305 310 315 320
Gly Asp Pro Asn Val Pro Asn Tyr Leu Lys Pro Gly Asn Ser Pro Trp
325 330 335
Val Gln Gly Gln Ile Asp Phe Gln Asn Cys Leu Asp Ser Gly Lys Thr
340 345 350
Ile Glu Gln Cys Arg Glu Glu Leu Asn
355 360
<210>361
<211>556
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(526)
<223>RXA03377
<400>361
ttcctctcca gggcacaatt tacccccatt gaactgcatc aatgggggtt catcatttcc 60
tccaaagtgc aaaaatacac gcttacgtac actgtagatc atg cta cag agc ccg 115
Met Leu Gln Ser Pro
1 5
aag aga gcc atc ggc ctg tcc gcc gca ttg ctc acg ctc acc gcg tgt l63
Lys Arg Ala Ile Gly Leu Ser Ala Ala Leu Leu Thr Leu Thr Ala Cys
10 15 20
ggg gca aac gac gag ctt tcc agt tat ctc acc tcc att gag aca gct 211
Gly Ala Asn Asp Glu Leu Ser Ser Tyr Leu Thr Ser Ile Glu Thr Ala
25 30 35
aca gct caa gtt tcc ctc aac gac att tac aac gat caa tgg tcc gaa 259
Thr Ala Gln Val Ser Leu Asn Asp Ile Tyr Asn Asp Gln Trp Ser Glu
40 45 50
ttt gca gtc gtt tgc ccc tac acg cca agt gac tat gcg aag gcg gaa 307
Phe Ala Val Val Cys Pro Tyr Thr Pro Ser Asp Tyr Ala Lys Ala Glu
55 60 65
ctt gac gtc gga gat acc ccc tgg cca gat aag tgg cca gac gaa caa 355
Leu Asp Val Gly Asp Thr Pro Trp Pro Asp Lys Trp Pro Asp Glu Gln
70 75 80 85
acc aat ttt ctt ctc cta aaa agt gac acg ggc gaa tac aaa tgg gtg 403
Thr Asn Phe Leu Leu Leu Lys Ser Asp Thr Gly Glu Tyr Lys Trp Val
90 95 100
cga tat cgc agg tca aac ctc gac ttt tgc tct gaa cca agg att gac 451
Arg Tyr Arg Arg Ser Asn Leu Asp Phe Cys Ser Glu Pro Arg Ile Asp
105 110 115
ttc acg ctc ttc ccc act gct gca acg ctg gaa ttc acc gct aac gat 499
Phe Thr Leu Phe Pro Thr Ala Ala Thr Leu Glu Phe Thr Ala Asn Asp
120 125 130
ggc tgg att ctc gag tct gta cac cct tgatcaaagc ccgagtgttc 546
Gly Trp Ile Leu Glu Ser Val His Pro
135 140
cgtagattaa 556
<210>362
<211>142
<212>PRT
<213>谷氨酸棒状杆菌
<400>362
Met Leu Gln Ser Pro Lys Arg Ala Ile Gly Leu Ser Ala Ala Leu Leu
1 5 10 15
Thr Leu Thr Ala Cys Gly Ala Asn Asp Glu Leu Ser Ser Tyr Leu Thr
20 25 30
Ser Ile Glu Thr Ala Thr Ala Gln Val Ser Leu Asn Asp Ile Tyr Asn
35 40 45
Asp Gln Trp Ser Glu Phe Ala Val Val Cys Pro Tyr Thr Pro Ser Asp
50 55 60
Tyr Ala Lys Ala Glu Leu Asp Val Gly Asp Thr Pro Trp Pro Asp Lys
65 70 75 80
Trp Pro Asp Glu Gln Thr Asn Phe Leu Leu Leu Lys Ser Asp Thr Gly
85 90 95
Glu Tyr Lys Trp Val Arg Tyr Arg Arg Ser Asn Leu Asp Phe Cys Ser
100 105 110
Glu Pro Arg Ile Asp Phe Thr Leu Phe Pro Thr Ala Ala Thr Leu Glu
115 120 125
Phe Thr Ala Asn Asp Gly Trp Ile Leu Glu Ser Val His Pro
130 135 140
<210>363
<211>304
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(274)
<223>RXA03463
<400>363
atagttcgcg ctgttttaag aggttgagat tctcaaacgg gtgtttgtat agatcacatt 60
gcgatcatgc cttagacgcg attctgagag ggcaagtttt ttg agt tcc ggt tcc 115
Leu Ser Ser Gly Ser
1 5
aat ttt aag ctc gac caa acc aca gtg ccc acg aac tgg aaa ttc gac 163
Asn Phe Lys Leu Asp Gln Thr Thr Val Pro Thr Asn Trp Lys Phe Asp
10 15 20
aac tct ggg cat ttg tgt ctg gtt tca agc cgt cga aac cag acc acg 211
Asn Ser Gly His Leu Cys Leu Val Ser Ser Arg Arg Asn Gln Thr Thr
25 30 35
ctg ccc atg aac cgg gaa aac ctg agc cgt ggg cag tgt ggt ttg gtc 259
Leu Pro Met Asn Arg Glu Asn Leu Ser Arg Gly Gln Cys Gly Leu Val
40 45 50
atg gcg ttc gaa tgc tgattcgggc cagacaggcg tgacaagatt 304
Met Ala Phe Glu Cys
55
<210>364
<211>58
<212>PRT
<213>谷氨酸棒状杆菌
<400>364
Leu Ser Ser Gly Ser Asn Phe Lys Leu Asp Gln Thr Thr Val Pro Thr
1 5 10 15
Asn Trp Lys Phe Asp Asn Ser Gly His Leu Cys Leu Val Ser Ser Arg
20 25 30
Arg Asn Gln Thr Thr Leu Pro Met Asn Arg Glu Asn Leu Ser Arg Gly
35 40 45
Gln Cys Gly Leu Val Met Ala Phe Glu Cys
50 55
<210>365
<211>496
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(466)
<223>RXA03506
<400>365
cgcacaaagt gggtgtgaaa actccgcttc atgaccttat ttatgctggt cttaagctga 60
aagaagagga aaattcactt tagggataga atcaagatcc atg atc gtt aca tcc 115
Met Ile Val Thr Ser
1 5
acc gcg cgc gtt tct ata gat cgt ccc tcg cgc tat gcc aaa cag tta 163
Thr Ala Arg Val Ser Ile Asp Arg Pro Ser Arg Tyr Ala Lys Gln Leu
10 15 20
act agc cat ttg ggt gaa aaa ctg agc aca agc tgg gac ccg gag gca 211
Thr Ser His Leu Gly Glu Lys Leu Ser Thr Ser Trp Asp Pro Glu Ala
25 30 35
gaa cgg ggg tct gtt gtc ttg gtg gga aag ggc cca gac cac gaa gac 259
Glu Arg Gly Ser Val Val Leu Val Gly Lys Gly Pro Asp His Glu Asp
40 45 50
cag cgg ttt gct ttt gat gga tct gct acc tgt gat ctg gtt gcc ggc 307
Gln Arg Phe Ala Phe Asp Gly Ser Ala Thr Cys Asp Leu Val Ala Gly
55 60 65
gac ggc gtg ctg ttg ctc cac att gaa tcg ccc gcc agc ctg gtt gag 355
Asp Gly Val Leu Leu Leu His Ile Glu Ser Pro Ala Ser Leu Val Glu
70 75 80 85
cgt ttt gaa aat gtc atc ggc ggc cat ctg gtc ggc ttc gct cgg cgc 403
Arg Phe Glu Asn Val Ile Gly Gly His Leu Val Gly Phe Ala Arg Arg
90 95 100
gac gag atg aag att gct ttt cga cgt ggt gat gga tca gaa ggg ctg 451
Asp Glu Met Lys Ile Ala Phe Arg Arg Gly Asp Gly Ser Glu Gly Leu
105 110 115
tct ttt gag tcc gtc taacataatc cgcatcgcag ctgttctaat 496
Ser Phe Glu Ser Val
120
<210>366
<211>122
<212>PRT
<213>谷氨酸棒状杆菌
<400>366
Met Ile Val Thr Ser Thr Ala Arg Val Ser Ile Asp Arg Pro Ser Arg
1 5 10 15
Tyr Ala Lys Gln Leu Thr Ser His Leu Gly Glu Lys Leu Ser Thr Ser
20 25 30
Trp Asp Pro Glu Ala Glu Arg Gly Ser Val Val Leu Val Gly Lys Gly
35 40 45
Pro Asp His Glu Asp Gln Arg Phe Ala Phe Asp Gly Ser Ala Thr Cys
50 55 60
Asp Leu Val Ala Gly Asp Gly Val Leu Leu Leu His Ile Glu Ser Pro
65 70 75 80
Ala Ser Leu Val Glu Arg Phe Glu Asn Val Ile Gly Gly His Leu Val
85 90 95
Gly Phe Ala Arg Arg Asp Glu Met Lys Ile Ala Phe Arg Arg Gly Asp
100 105 110
Gly Ser Glu Gly Leu Ser Phe Glu Ser Val
115 120
<210>367
<211>757
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(727)
<223>RXA03523
<400>367
tctgtaattg cggtaattcg cttagttgaa gctgttgaaa ttacgagcac ttctaaatac 60
ctggtagatg gattatttag ccccggaagg ggctattttt gtg tct aaa ccc gag 115
Val Ser Lys Pro Glu
1 5
ggt aat ttg gga gga gcg tta agt act act gac agg tta aac aaa ggt 163
Gly Asn Leu Gly Gly Ala Leu Ser Thr Thr Asp Arg Leu Asn Lys Gly
10 15 20
ttt gcc agg tgt cgg gac ccg ctc gaa gct gaa aat gtc tac tgg gaa 211
Phe Ala Arg Cys Arg Asp Pro Leu Glu Ala Glu Asn Val Tyr Trp Glu
25 30 35
tac cgc aac aat gga gtc tgg atc agg att aac acc gac aac aac ttc 259
Tyr Arg Asn Asn Gly Val Trp Ile Arg Ile Asn Thr Asp Asn Asn Phe
40 45 50
cag gtc act acg gca gat gga ctc acc tcc tta acc gtg act gat acg 307
Gln Val Thr Thr Ala Asp Gly Leu Thr Ser Leu Thr Val Thr Asp Thr
55 60 65
gca gtt gat cag tct gga gca agg tta tgt gcc gtt gct act aat gct 355
Ala Val Asp Gln Ser Gly Ala Arg Leu Cys Ala Val Ala Thr Asn Ala
70 75 80 85
tct ggc ccc acg atg tcg agg gaa gct gtt ctc acg gtg cgt tct gca 403
Ser Gly Pro Thr Met Ser Arg Glu Ala Val Leu Thr Val Arg Ser Ala
90 95 100
acc gag atc cct gat gat ggt ctg agc att gat gat gtt gta ctg cgt 451
Thr Glu Ile Pro Asp Asp Gly Leu Ser Ile Asp Asp Val Val Leu Arg
105 110 115
tgg ggt att agt gag gaa gta caa tca cga cca cta ttt ggt gac tcc 499
Trp Gly Ile Ser Glu Glu Val Gln Ser Arg Pro Leu Phe Gly Asp Ser
120 125 130
aac tac cta tcc gct ggt gtg tct gat gga tct gca ttg acc tac gag 547
Asn Tyr Leu Ser Ala Gly Val Ser Asp Gly Ser Ala Leu Thr Tyr Glu
135 140 145
tca act gat agg gat gta cgc atc atc cac agg gtg ctg ttg caa agt 595
Ser Thr Asp Arg Asp Val Arg Ile Ile His Arg Val Leu Leu Gln Ser
150 155 160 165
tgg gcc agg gtc gaa aag ccc aac ctc aag aca tta gtt gtc ctt gct 643
Trp Ala Arg Val Glu Lys Pro Asn Leu Lys Thr Leu Val Val Leu Ala
170 175 180
ccg ggg tgc tta agc tgt ctc gaa tat ccg gct gac gat cac cgc gtc 691
Pro Gly Cys Leu Ser Cys Leu Glu Tyr Pro Ala Asp Asp His Arg Val
185 190 195
tgg gtt cgg gtg ccc gtg agg tca aac atc gtt ccc tgaccctttc 737
Trp Val Arg Val Pro Val Arg Ser Asn Ile Val Pro
200 205
cgcaatgaat gcatcgcctc 757
<210>368
<211>209
<212>PRT
<213>谷氨酸棒状杆菌
<400>368
Val Ser Lys Pro Glu Gly Asn Leu Gly Gly Ala Leu Ser Thr Thr Asp
1 5 10 15
Arg Leu Asn Lys Gly Phe Ala Arg Cys Arg Asp Pro Leu Glu Ala Glu
20 25 30
Asn Val Tyr Trp Glu Tyr Arg Asn Asn Gly Val Trp Ile Arg Ile Asn
35 40 45
Thr Asp Asn Asn Phe Gln Val Thr Thr Ala Asp Gly Leu Thr Ser Leu
50 55 60
Thr Val Thr Asp Thr Ala Val Asp Gln Ser Gly Ala Arg Leu Cys Ala
65 70 75 80
Val Ala Thr Asn Ala Ser Gly Pro Thr Met Ser Arg Glu Ala Val Leu
85 90 95
Thr Val Arg Ser Ala Thr Glu Ile Pro Asp Asp Gly Leu Ser Ile Asp
100 105 110
Asp Val Val Leu Arg Trp Gly Ile Ser Glu Glu Val Gln Ser Arg Pro
115 120 125
Leu Phe Gly Asp Ser Asn Tyr Leu Ser Ala Gly Val Ser Asp Gly Ser
130 135 140
Ala Leu Thr Tyr Glu Ser Thr Asp Arg Asp Val Arg Ile Ile His Arg
145 150 155 160
Val Leu Leu Gln Ser Trp Ala Arg Val Glu Lys Pro Asn Leu Lys Thr
165 170 175
Leu Val Val Leu Ala Pro Gly Cys Leu Ser Cys Leu Glu Tyr Pro Ala
180 185 190
Asp Asp His Arg Val Trp Val Arg Val Pro Val Arg Ser Asn Ile Val
195 200 205
Pro
<210>369
<211>559
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(529)
<223>RXA03572
<400>369
accttggtgc actgcagggg ttgttttcta tgtgccaacg agttcttaag aaacacaacg 60
acagcaaagc tgcgttcggg agcatgtgtg aaccctcgct gtg ccc gca ctt gcc 115
Val Pro Ala Leu Ala
1 5
tgc ata cag ctc act caa caa cac aga aag aac ctt tta cca tgc tca 163
Cys Ile Gln Leu Thr Gln Gln His Arg Lys Asn Leu Leu Pro Cys Ser
10 15 20
atg cac tca acg ctg tcc ttg atg ttg tcg aat ctg ctc aag ggc tgg 211
Met His Ser Thr Leu Ser Leu Met Leu Ser Asn Leu Leu Lys Gly Trp
25 30 35
tct ttg tcg tca tca ctg cta gcc tgc tca ctc ttg ctg tca tct aca 259
Ser Leu Ser Ser Ser Leu Leu Ala Cys Ser Leu Leu Leu Ser Ser Thr
40 45 50
tca aga act atc ccc acg cgc cac agc ctg cta ccc aac aca cca ctg 307
Ser Arg Thr Ile Pro Thr Arg His Ser Leu Leu Pro Asn Thr Pro Leu
55 60 65
acc aac caa ctg tgc cag agc cgc tgc aga ttc ttg aca acc ctg ctc 355
Thr Asn Gln Leu Cys Gln Ser Arg Cys Arg Phe Leu Thr Thr Leu Leu
70 75 80 85
aga caa ctg ctg ctg gtc tac gcc atg acc ttc ata gcg atc tct acg 403
Arg Gln Leu Leu Leu Val Tyr Ala Met Thr Phe Ile Ala Ile Ser Thr
90 95 100
ccg aag aaa aga gcg cat aat ctg tgt ctc gat gtt gcc tgg gat gct 451
Pro Lys Lys Arg Ala His Asn Leu Cys Leu Asp Val Ala Trp Asp Ala
105 110 115
gat gaa aac ctc cct gtt gtc gtt gaa gtc aat cct gta gcg atg cct 499
Asp Glu Asn Leu Pro Val Val Val Glu Val Asn Pro Val Ala Met Pro
120 125 130
ggg tgt tgc gcc tcg ggc gca gct ggg tgc taacttagca ccgcatcaac 549
Gly Cys Cys Ala Ser Gly Ala Ala Gly Cys
135 140
gcagcgaagc 559
<210>370
<211>143
<212>PRT
<213>谷氨酸棒状杆菌
<400>370
Val Pro Ala Leu Ala Cys Ile Gln Leu Thr Gln Gln His Arg Lys Asn
1 5 10 15
Leu Leu Pro Cys Ser Met His Ser Thr Leu Ser Leu Met Leu Ser Asn
20 25 30
Leu Leu Lys Gly Trp Ser Leu Ser Ser Ser Leu Leu Ala Cys Ser Leu
35 40 45
Leu Leu Ser Ser Thr Ser Arg Thr Ile Pro Thr Arg His Ser Leu Leu
50 55 60
Pro Asn Thr Pro Leu Thr Asn Gln Leu Cys Gln Ser Arg Cys Arg Phe
65 70 75 80
Leu Thr Thr Leu Leu Arg Gln Leu Leu Leu Val Tyr Ala Met Thr Phe
85 90 95
Ile Ala Ile Ser Thr Pro Lys Lys Arg Ala His Asn Leu Cys Leu Asp
100 105 110
Val Ala Trp Asp Ala Asp Glu Asn Leu Pro Val Val Val Glu Val Asn
115 120 125
Pro Val Ala Met Pro Gly Cys Cys Ala Ser Gly Ala Ala Gly Cys
130 135 140
<210>371
<211>505
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(475)
<223>RXA03 577
<400>371
aattttgcaa gcgttccgag gcggccttgc aacgtcttac cccaaatata atcaaattag 60
agacctagtc gactcaagta gttaagagga aacaacaata atg tta cga aaa gta 115
Met Leu Arg Lys Val
1 5
tcc att gca ttg gtc gca tcc gcc gct atc ttg gta tct gta cct ttc 163
Ser Ile Ala Leu Val Ala Ser Ala Ala Ile Leu Val Ser Val Pro Phe
10 15 20
gca cag gca tcg acc tca gtg gca ctg tcg tca gaa agc tca gat tct 211
Ala Gln Ala Ser Thr Ser Val Ala Leu Ser Ser Glu Ser Ser Asp Ser
25 30 35
ctg gga agt att cca aac gat tta att ggg ggt cta gcc ggt tac ttt 259
Leu Gly Ser Ile Pro Asn Asp Leu Ile Gly Gly Leu Ala Gly Tyr Phe
40 45 50
aat aaa gat ctt acg gag gaa cag cgc gat gct att cgc ttc ggc ccc 307
Asn Lys Asp Leu Thr Glu Glu Gln Arg Asp Ala Ile Arg Phe Gly Pro
55 60 65
acc aca gca caa gaa gca gaa cag tgc tta gca aaa tac gga ctt ggc 355
Thr Thr Ala Gln Glu Ala Glu Gln Cys Leu Ala Lys Tyr Gly Leu Gly
70 75 80 85
ggt gaa ggc ccg tgg cct act atc gcg gtg tac cct aac tgc tct ttc 403
Gly Glu Gly Pro Trp Pro Thr Ile Ala Val Tyr Pro Asn Cys Ser Phe
90 95 100
gag aca att gaa caa gat ttc tat gat caa gcg gta gcc gag act ctc 451
Glu Thr Ile Glu Gln Asp Phe Tyr Asp Gln Ala Val Ala Glu Thr Leu
l05 110 115
gca gca ata ggc atc ggg agc tct taaacaatca aaggtacaac ccaaaaaaca 505
Ala Ala Ile Gly Ile Gly Ser Ser
120 125
<210>372
<211>125
<212>PRT
<213>谷氨酸棒状杆菌
<400>372
Met Leu Arg Lys Val Ser Ile Ala Leu Val Ala Ser Ala Ala Ile Leu
1 5 10 15
Val Ser Val Pro Phe Ala Gln Ala Ser Thr Ser Val Ala Leu Ser Ser
20 25 30
Glu Ser Ser Asp Ser Leu Gly Ser Ile Pro Asn Asp Leu Ile Gly Gly
35 40 45
Leu Ala Gly Tyr Phe Asn Lys Asp Leu Thr Glu Glu Gln Arg Asp Ala
50 55 60
Ile Arg Phe Gly Pro Thr Thr Ala Gln Glu Ala Glu Gln Cys Leu Ala
65 70 75 80
Lys Tyr Gly Leu Gly Gly Glu Gly Pro Trp Pro Thr Ile Ala Val Tyr
85 90 95
Pro Asn Cys Ser Phe Glu Thr Ile Glu Gln Asp Phe Tyr Asp Gln Ala
100 105 110
Val Ala Glu Thr Leu Ala Ala Ile Gly Ile Gly Ser Ser
115 120 125
<210>373
<211>379
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(349)
<223>RXA03584
<400>373
gcacatcatt tagacctgat ctgtctcact tgtggtcact ccccgtaggg tgggtagctc 60
cggggcttga gggtttcgtg gagcgtaatt cagaggcagc ttg aac cac cgc tct 115
Leu Asn His Arg Ser
1 5
ttg agc cgg aat ctc cac tct tta ggg tgg aga gga tat caa aag atg 163
Leu Ser Arg Asn Leu His Ser Leu Gly Trp Arg Gly Tyr Gln Lys Met
10 15 20
gga gat cat ggt cga aaa agt gag ttc gca gga atc tcc cgg agg aag 211
Gly Asp His Gly Arg Lys Ser Glu Phe Ala Gly Ile Ser Arg Arg Lys
25 30 35
tcg cac cgg ctt atc acg tcg att tat ggg gct tgt tgc cgg cag gag 259
Ser His Arg Leu Ile Thr Ser Ile Tyr Gly Ala Cys Cys Arg Gln Glu
40 45 50
tca ctg tat ggg ttt gca tgt aaa agt aaa aaa ata acc ccc gca gca 307
Ser Leu Tyr Gly Phe Ala Cys Lys Ser Lys Lys Ile Thr Pro Ala Ala
55 60 65
gct tgt tta agc tac tac gag ggt tat ttt ttg tgg atg tgt 349
Ala Cys Leu Ser Tyr Tyr Glu Gly Tyr Phe Leu Trp Met Cys
70 75 80
tagtcaaatg gcgcgagttc ccatactgca 379
<210>374
<211>83
<212>PRT
<213>谷氨酸棒状杆菌
<400>374
Leu Asn His Arg Ser Leu Ser Arg Asn Leu His Ser Leu Gly Trp Arg
1 5 10 15
Gly Tyr Gln Lys Met Gly Asp His Gly Arg Lys Ser Glu Phe Ala Gly
20 25 30
Ile Ser Arg Arg Lys Ser His Arg Leu Ile Thr Ser Ile Tyr Gly Ala
35 40 45
Cys Cys Arg Gln Glu Ser Leu Tyr Gly Phe Ala Cys Lys Ser Lys Lys
50 55 60
Ile Thr Pro Ala Ala Ala Cys Leu Ser Tyr Tyr Glu Gly Tyr Phe Leu
65 70 75 80
Trp Met Cys
<210>375
<211>3919
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3889)
<223>RXA03591
<400>375
aggactgctc cgcaaacctc taacaacacc tgctactccc cctgcagcgc cgctcaaccc 60
cacaccaccc accccttaac aaccacaaga aagatttatc atg tcc gct cca acc 115
Met Ser Ala Pro Thr
1 5
atc tac ccc ggc acc aaa aca tct att gat ccg atc acc atg gat gac 163
Ile Tyr Pro Gly Thr Lys Thr Ser Ile Asp Pro Ile Thr Met Asp Asp
10 15 20
gct cgc atc atc ttt ttc gat att gag tcg ctc cac aat att ttc acg 211
Ala Arg Ile Ile Phe Phe Asp Ile Glu Ser Leu His Asn Ile Phe Thr
25 30 35
gta gca acc tac gat tct ctg tcc cac cac gtc gat gtc ttt tac ctg 259
Val Ala Thr Tyr Asp Ser Leu Ser His His Val Asp Val Phe Tyr Leu
40 45 50
ctc gat cac aca acc agc cct cag atc acg gtg ctg ccg cac tca atg 307
Leu Asp His Thr Thr Ser Pro Gln Ile Thr Val Leu Pro His Ser Met
55 60 65
gat tat ttc gat caa acg cgc agc gat gct gtt atg gct gcc atc att 355
Asp Tyr Phe Asp Gln Thr Arg Ser Asp Ala Val Met Ala Ala Ile Ile
70 75 80 85
gag caa aac cct gcg ttc gca gaa att aaa ggc tca ccc att aca acc 403
Glu Gln Asn Pro Ala Phe Ala Glu Ile Lys Gly Ser Pro Ile Thr Thr
90 95 100
gca gat gta gcc ctc cac aat ctc ggt gac acc aac gcc aac cga cgc 451
Ala Asp Val Ala Leu His Asn Leu Gly Asp Thr Asn Ala Asn Arg Arg
105 110 115
tgg cag tct aac gtg ctg ctt gcc cgg cta ctc ggg ggt att agt gtg 499
Trp Gln Ser Asn Val Leu Leu Ala Arg Leu Leu Gly Gly Ile Ser Val
120 125 130
cgc gga gag gta cct gag cac cag agc cac aac cat ctc gcc aag cag 547
Arg Gly Glu Val Pro Glu His Gln Ser His Asn His Leu Ala Lys Gln
135 140 145
ttt gcc gag gca acc ttg gtc acc agg gac ttc gat gtg aat tat gat 595
Phe Ala Glu Ala Thr Leu Val Thr Arg Asp Phe Asp Val Asn Tyr Asp
150 155 160 165
cca aca agc gct cac cct ttt act gct ggc ttc aac tcg atc aac tat 643
Pro Thr Ser Ala His Pro Phe Thr Ala Gly Phe Asn Ser Ile Asn Tyr
170 175 180
gac acc acc ttg ctc agc ctg tac ttc gca atg ttg acc tca aat atc 691
Asp Thr Thr Leu Leu Ser Leu Tyr Phe Ala Met Leu Thr Ser Asn Ile
185 190 195
gga agt aca ccg acg tat ttc ccg gtg atc acc gca cag gaa ctt cgt 739
Gly Ser Thr Pro Thr Tyr Phe Pro Val Ile Thr Ala Gln Glu Leu Arg
200 205 210
gcg cat aac gac aag ctc ttt agc cct gag ttc atc aaa aac atg cca 787
Ala His Asn Asp Lys Leu Phe Ser Pro Glu Phe Ile Lys Asn Met Pro
215 220 225
aag tat ctc tgg gat cgc gac agc ggt gct gga ctc aga gct gca tcg 835
Lys Tyr Leu Trp Asp Arg Asp Ser Gly Ala Gly Leu Arg Ala Ala Ser
230 235 240 245
ggt ttc cgc aac gcc atg ctc aaa tcg ggt cgc cac att gat att caa 883
Gly Phe Arg Asn Ala Met Leu Lys Ser Gly Arg His Ile Asp Ile Gln
250 255 260
cgc ctc aat gaa aaa cag ctc ttt gtt gga ctc aag cgc ctg ctt ggt 931
Arg Leu Asn Glu Lys Gln Leu Phe Val Gly Leu Lys Arg Leu Leu Gly
265 270 275
ctc ctc ggt cac cag att ctc gaa tct gat cgt ctc tct ggt gat gat 979
Leu Leu Gly His Gln Ile Leu Glu Ser Asp Arg Leu Ser Gly Asp Asp
280 285 290
gcc cat gtt gat act aac gag gat gta ctt gat ctc att gcc tac aac 1027
Ala His Val Asp Thr Asn Glu Asp Val Leu Asp Leu Ile Ala Tyr Asn
295 300 305
gtc tca gac gtg gtg ggc acc aga ctg ctc gct gag gac ccg gtg tac 1075
Val Ser Asp Val Val Gly Thr Arg Leu Leu Ala Glu Asp Pro Val Tyr
310 315 320 325
tcc ggc tct ttc gat ctg cgg gca ggt cta ctg agc acc tac cca gag 1123
Ser Gly Ser Phe Asp Leu Arg Ala Gly Leu Leu Ser Thr Tyr Pro Glu
330 335 340
act gtt ttt gat cat gat ggt act ttc cgt cag cca tcc acg cag atg 1171
Thr Val Phe Asp His Asp Gly Thr Phe Arg Gln Pro Ser Thr Gln Met
345 350 355
cgt aaa gat cgc cta acg att aat acc tca tca gct cag ttc gca gcg 1219
Arg Lys Asp Arg Leu Thr Ile Asn Thr Ser Ser Ala Gln Phe Ala Ala
360 365 370
cgt att ttg gcg cca tat cgc cca ctc cgc gat gtc cct gat gcg att 1267
Arg Ile Leu Ala Pro Tyr Arg Pro Leu Arg Asp Val Pro Asp Ala Ile
375 380 385
ggc gac atg ccg gtg gtg tct tac ttg tac ccg gat gca gca gtc gcc 1315
Gly Asp Met Pro Val Val Ser Tyr Leu Tyr Pro Asp Ala Ala Val Ala
390 395 400 405
gaa gca aca ggt caa aaa caa gtc aac gtg ctt gat gag tca aag aag 1363
Glu Ala Thr Gly Gln Lys Gln Val Asn Val Leu Asp Glu Ser Lys Lys
410 415 420
ttc ttc tat gac aac atc acc gac ccg gaa gca cgt gct gcc ttt gat 1411
Phe Phe Tyr Asp Asn Ile Thr Asp Pro Glu Ala Arg Ala Ala Phe Asp
425 430 435
gag gtc ttt gct ttt tac gct gat att gag ggt cgc aac ttc aac agt 1459
Glu Val Phe Ala Phe Tyr Ala Asp Ile Glu Gly Arg Asn Phe Asn Ser
440 445 450
cac aat gag gct att gat acc cag att aac caa tta cgt gct tat ctc 1507
His Asn Glu Ala Ile Asp Thr Gln Ile Asn Gln Leu Arg Ala Tyr Leu
455 460 465
aac cag gtt gtc gca ttc gat gca gct ggg tat gcg ctc tat gat gta 1555
Asn Gln Val Val Ala Phe Asp Ala Ala Gly Tyr Ala Leu Tyr Asp Val
470 475 480 485
cgt aca cgt ttt gag cag atc ttc ccc aag gat cgc agc tac atc aac 1603
Arg Thr Arg Phe Glu Gln Ile Phe Pro Lys Asp Arg Ser Tyr Ile Asn
490 495 500
gat gct acg gat atg acc cct cgc gca gta tcg agc ttt gac gat ctg 1651
Asp Ala Thr Asp Met Thr Pro Arg Ala Val Ser Ser Phe Asp Asp Leu
505 510 515
gtt gca ctc tgt gat gat att cgc ggt gta ctt gat cga ggt tta gag 1699
Val Ala Leu Cys Asp Asp Ile Arg Gly Val Leu Asp Arg Gly Leu Glu
520 525 530
atc tca tct ccg aat cat cat gag atg gtg gat gct atg cgc aag cag 1747
Ile Ser Ser Pro Asn His His Glu Met Val Asp Ala Met Arg Lys Gln
535 540 545
ctg cac tat att cag gca ttt tac cgt gcc tgg gga ccc att caa cgc 1795
Leu His Tyr Ile Gln Ala Phe Tyr Arg Ala Trp Gly Pro Ile Gln Arg
550 555 560 565
cgc ttc aat gac gct gac cca gcg gtg acc cat ccg cat ctc aca gtg 1843
Arg Phe Asn Asp Ala Asp Pro Ala Val Thr His Pro His Leu Thr Val
570 575 580
atc tac cca ccg ctc acc cct gca tcc gca gag aaa ttc aac aag atc 1891
Ile Tyr Pro Pro Leu Thr Pro Ala Ser Ala Glu Lys Phe Asn Lys Ile
585 590 595
acc tca gtc gct gct gtg agc aag cgc cca acc acc ctg ccg tat ttc 1939
Thr Ser Val Ala Ala Val Ser Lys Arg Pro Thr Thr Leu Pro Tyr Phe
600 605 610
cgt gca gat ggt tca cct act cgt ggc ttt gct aac ttc tcc aca gga 1987
Arg Ala Asp Gly Ser Pro Thr Arg Gly Phe Ala Asn Phe Ser Thr Gly
615 620 625
ggt att cac ggt gcg gaa tac aac gaa gat cgt ttt gac cat gat caa 2035
Gly Ile His Gly Ala Glu Tyr Asn Glu Asp Arg Phe Asp His Asp Gln
630 635 640 645
gat cta cac aca gtg gca acc cgt gaa ttc ttc gcc att ctt gat gcg 2083
Asp Leu His Thr Val Ala Thr Arg Glu Phe Phe Ala Ile Leu Asp Ala
650 655 660
aca tta gcg gct ctc tac gca gcc cac caa gcc gaa cct gaa tcg gcg 2131
Thr Leu Ala Ala Leu Tyr Ala Ala His Gln Ala Glu Pro Glu Ser Ala
665 670 675
gac tac cag att gca caa gac gca ctc gcc tgg gca aag aat gta ctt 2179
Asp Tyr Gln Ile Ala Gln Asp Ala Leu Ala Trp Ala Lys Asn Val Leu
680 685 690
agc gat caa aaa ctc atc gct aag tcg cct cag tta tac aac ccc gaa 2227
Ser Asp Gln Lys Leu Ile Ala Lys Ser Pro Gln Leu Tyr Asn Pro Glu
695 700 705
acc ggg gtc acc tat gag tgg gag ttt gtc gct caa gca gca tgg tgg 2275
Thr Gly Val Thr Tyr Glu Trp Glu Phe Val Ala Gln Ala Ala Trp Trp
710 715 720 725
att cgc aac aaa ccg gtt gag gtt att tta cca aca ggt gaa tca atg 2323
Ile Arg Asn Lys Pro Val Glu Val Ile Leu Pro Thr Gly Glu Ser Met
730 735 740
acc gta aaa cac aaa tct gta ttg gca tct gcg tcc tac cca ctc cga 2371
Thr Val Lys His Lys Ser Val Leu Ala Ser Ala Ser Tyr Pro Leu Arg
745 750 755
gac aac gtg gcg tac tgg cgt agc gaa ccc aaa aca cca cag ctg ttt 2419
Asp Asn Val Ala Tyr Trp Arg Ser Glu Pro Lys Thr Pro Gln Leu Phe
760 765 770
ccc gta gca aaa tct ggt ggt tcc tcg ctt gag aag aag tac aac tac 2467
Pro Val Ala Lys Ser Gly Gly Ser Ser Leu Glu Lys Lys Tyr Asn Tyr
775 780 785
acc tct gtg ggt act gct att cac gag gac ttt agc agt tac tat ccg 2515
Thr Ser Val Gly Thr Ala Ile His Glu Asp Phe Ser Ser Tyr Tyr Pro
790 795 800 805
ctc ttg ctc acc aat atg gct gca ttt acc aat gct gac tta ggt att 2563
Leu Leu Leu Thr Asn Met Ala Ala Phe Thr Asn Ala Asp Leu Gly Ile
810 815 820
gat gag aaa acc ggg cgc cct cgt gac cgc tac cgt gaa atc tac gaa 2611
Asp Glu Lys Thr Gly Arg Pro Arg Asp Arg Tyr Arg Glu Ile Tyr Glu
825 830 835
caa aaa gag atc tat ggc gca cag cgc aaa gac ccc tcc att gat gaa 2659
Gln Lys Glu Ile Tyr Gly Ala Gln Arg Lys Asp Pro Ser Ile Asp Glu
840 845 850
gaa aca aag caa cgt cta ggg att ttg cgc gaa ggt acc aag ttg att 2707
Glu Thr Lys Gln Arg Leu Gly Ile Leu Arg Glu Gly Thr Lys Leu Ile
855 860 865
ctt aac tcg gcc acc ggt gct gct gat gct ggt cac gac acc ccg atc 2755
Leu Asn Ser Ala Thr Gly Ala Ala Asp Ala Gly His Asp Thr Pro Ile
870 875 880 885
ctc atg aac aat cgc gtt att gcc atg agg att att ggg cag cta ttt 2803
Leu Met Asn Asn Arg Val Ile Ala Met Arg Ile Ile Gly Gln Leu Phe
890 895 900
tct tgg aga att ggg caa gct caa tca ctg gct ggt gca acg att att 2851
Ser Trp Arg Ile Gly Gln Ala Gln Ser Leu Ala Gly Ala Thr Ile Ile
905 910 915
tcc act aac act gat ggc ctg tat tcc gta ctc gat atg ggg act aac 2899
Ser Thr Asn Thr Asp Gly Leu Tyr Ser Val Leu Asp Met Gly Thr Asn
920 925 930
cag cgc gtt ctt gat gaa cac gct aca gcc atc ggt gtg caa att gag 2947
Gln Arg Val Leu Asp Glu His Ala Thr Ala Ile Gly Val Gln Ile Glu
935 940 945
cct gaa gaa ctg gat att gtc tcc aag gat tcc aac tca cgt gcc gaa 2995
Pro Glu Glu Leu Asp Ile Val Ser Lys Asp Ser Asn Ser Arg Ala Glu
950 955 960 965
ttt ctc ggc aat ggc tac atc aat gcg gcc ggt gac ttg gcg tgt tgg 3043
Phe Leu Gly Asn Gly Tyr Ile Asn Ala Ala Gly Asp Leu Ala Cys Trp
970 975 980
gat gga ccc aac tct cgg aac tca cta gat cac ccg gcc ttt gtt gac 3091
Asp Gly Pro Asn Ser Arg Asn Ser Leu Asp His Pro Ala Phe Val Asp
985 990 995
cat gtg ctg gtg aag tat ttc cag ctc gtc gtc aac aac act gtg cca 3139
His Val Leu Val Lys Tyr Phe Gln Leu Val Val Asn Asn Thr Val Pro
1000 1005 1010
gag att cca gaa aca cct gag ctt gag ggt gta ccg ctg gct ctt gat 3187
Glu Ile Pro Glu Thr Pro Glu Leu Glu Gly Val Pro Leu Ala Leu Asp
1015 1020 1025
caa ccc atg aac cgc cac gaa gtc tcg aag att gtc gcc act atg cac 3235
Gln Pro Met Asn Arg His Glu Val Ser Lys Ile Val Ala Thr Met His
1030 1035 1040 1045
aag gaa ttc gag ccg aaa aag ctg ctg tcg ttt tac caa aac att ttg 3283
Lys Glu Phe Glu Pro Lys Lys Leu Leu Ser Phe Tyr Gln Asn Ile Leu
1050 1055 1060
gcc tct tca cgc ggt tct aat acc ttc cta ttc tct gtc ccc tat atc 3331
Ala Ser Ser Arg Gly Ser Asn Thr Phe Leu Phe Ser Val Pro Tyr Ile
1065 1070 1075
cct gca aca gaa gga gaa gaa acc cac cct gca aca gat acc agc act 3379
Pro Ala Thr Glu Gly Glu Glu Thr His Pro Ala Thr Asp Thr Ser Thr
1080 1085 1090
att gcg aca cca acc ttg agc ttt gat gct tat ggt aat aag gcc gag 3427
Ile Ala Thr Pro Thr Leu Ser Phe Asp Ala Tyr Gly Asn Lys Ala Glu
1095 1100 1105
gtc atg ccg act caa tcc act gtg gat aag cgc gtg ccc tct ttg ctg 3475
Val Met Pro Thr Gln Ser Thr Val Asp Lys Arg Val Pro Ser Leu Leu
1110 1115 1120 1125
cag tat tac acg cgt aca ttc cat gtg cga caa gat acg cag cag gct 3523
Gln Tyr Tyr Thr Arg Thr Phe His Val Arg Gln Asp Thr Gln Gln Ala
1130 1135 1140
gtc ttt gat gtt att ggc gcc aac ccc gtg ctc atc gcc gca gca aaa 3571
Val Phe Asp Val Ile Gly Ala Asn Pro Val Leu Ile Ala Ala Ala Lys
1145 1150 1155
gct gca tcc ata tca ccg gct tct gct gat tca cgt aga aag aag ggc 3619
Ala Ala Ser Ile Ser Pro Ala Ser Ala Asp Ser Arg Arg Lys Lys Gly
1160 1165 1170
ctg gcc tcc acc aac gct gac cca gta gcc aag cat gta cta gaa att 3667
Leu Ala Ser Thr Asn Ala Asp Pro Val Ala Lys His Val Leu Glu Ile
1175 1180 1185
gca gga gct gat gtg gaa tcg ctg cgc cat gaa aag gat ctc aag gtc 3715
Ala Gly Ala Asp Val Glu Ser Leu Arg His Glu Lys Asp Leu Lys Val
1190 1195 1200 1205
acc aag cac act ggc caa gac cca gca ctg cct gtc gtg gtc ttt aac 3763
Thr Lys His Thr Gly Gln Asp Pro Ala Leu Pro Val Val Val Phe Asn
1210 1215 1220
caa acc att tgg cat aac ccc aac gac gat gtg att aat gca tta ctg 3811
Gln Thr Ile Trp His Asn Pro Asn Asp Asp Val Ile Asn Ala Leu Leu
1225 1230 1235
ggc gct att gac cag gat gcc tac att gac atg gcg atc tcg tcc tac 3859
Gly Ala Ile Asp Gln Asp Ala Tyr Ile Asp Met Ala Ile Ser Ser Tyr
1240 1245 1250
aac aag tcc tgg cac aac att atc ccg gct taactaccgc tgggtagacc 3909
Asn Lys Ser Trp His Asn Ile Ile Pro Ala
1255 1260
ccttgatgac 3919
<210>376
<211>1263
<212>PRT
<213>谷氨酸棒状杆菌
<400>376
Met Ser Ala Pro Thr Ile Tyr Pro Gly Thr Lys Thr Ser Ile Asp Pro
1 5 10 15
Ile Thr Met Asp Asp Ala Arg Ile Ile Phe Phe Asp Ile Glu Ser Leu
20 25 30
His Asn Ile Phe Thr Val Ala Thr Tyr Asp Ser Leu Ser His His Val
35 40 45
Asp Val Phe Tyr Leu Leu Asp His Thr Thr Ser Pro Gln Ile Thr Val
50 55 60
Leu Pro His Ser Met Asp Tyr Phe Asp Gln Thr Arg Ser Asp Ala Val
65 70 75 80
Met Ala Ala Ile Ile Glu Gln Asn Pro Ala Phe Ala Glu Ile Lys Gly
85 90 95
Ser Pro Ile Thr Thr Ala Asp Val Ala Leu His Asn Leu Gly Asp Thr
100 105 110
Asn Ala Asn Arg Arg Trp Gln Ser Asn Val Leu Leu Ala Arg Leu Leu
115 120 125
Gly Gly Ile Ser Val Arg Gly Glu Val Pro Glu His Gln Ser His Asn
130 135 140
His Leu Ala Lys Gln Phe Ala Glu Ala Thr Leu Val Thr Arg Asp Phe
145 150 155 160
Asp Val Asn Tyr Asp Pro Thr Ser Ala His Pro Phe Thr Ala Gly Phe
165 170 175
Asn Ser Ile Asn Tyr Asp Thr Thr Leu Leu Ser Leu Tyr Phe Ala Met
180 185 190
Leu Thr Ser Asn Ile Gly Ser Thr Pro Thr Tyr Phe Pro Val Ile Thr
195 200 205
Ala Gln Glu Leu Arg Ala His Asn Asp Lys Leu Phe Ser Pro Glu Phe
210 215 220
Ile Lys Asn Met Pro Lys Tyr Leu Trp Asp Arg Asp Ser Gly Ala Gly
225 230 235 240
Leu Arg Ala Ala Ser Gly Phe Arg Asn Ala Met Leu Lys Ser Gly Arg
245 250 255
His Ile Asp Ile Gln Arg Leu Asn Glu Lys Gln Leu Phe Val Gly Leu
260 265 270
Lys Arg Leu Leu Gly Leu Leu Gly His Gln Ile Leu Glu Ser Asp Arg
275 280 285
Leu Ser Gly Asp Asp Ala His Val Asp Thr Asn Glu Asp Val Leu Asp
290 295 300
Leu Ile Ala Tyr Asn Val Ser Asp Val Val Gly Thr Arg Leu Leu Ala
305 310 315 320
Glu Asp Pro Val Tyr Ser Gly Ser Phe Asp Leu Arg Ala Gly Leu Leu
325 330 335
Ser Thr Tyr Pro Glu Thr Val Phe Asp His Asp Gly Thr Phe Arg Gln
340 345 350
Pro Ser Thr Gln Met Arg Lys Asp Arg Leu Thr Ile Asn Thr Ser Ser
355 360 365
Ala Gln Phe Ala Ala Arg Ile Leu Ala Pro Tyr Arg Pro Leu Arg Asp
370 375 380
Val Pro Asp Ala Ile Gly Asp Met Pro Val Val Ser Tyr Leu Tyr Pro
385 390 395 400
Asp Ala Ala Val Ala Glu Ala Thr Gly Gln Lys Gln Val Asn Val Leu
405 410 415
Asp Glu Ser Lys Lys Phe Phe Tyr Asp Asn Ile Thr Asp Pro Glu Ala
420 425 430
Arg Ala Ala Phe Asp Glu Val Phe Ala Phe Tyr Ala Asp Ile Glu Gly
435 440 445
Arg Asn Phe Asn Ser His Asn Glu Ala Ile Asp Thr Gln Ile Asn Gln
450 455 460
Leu Arg Ala Tyr Leu Asn Gln Val Val Ala Phe Asp Ala Ala Gly Tyr
465 470 475 480
Ala Leu Tyr Asp Val Arg Thr Arg Phe Glu Gln Ile Phe Pro Lys Asp
485 490 495
Arg Ser Tyr Ile Asn Asp Ala Thr Asp Met Thr Pro Arg Ala Val Ser
500 505 510
Ser Phe Asp Asp Leu Val Ala Leu Cys Asp Asp Ile Arg Gly Val Leu
515 520 525
Asp Arg Gly Leu Glu Ile Ser Ser Pro Asn His His Glu Met Val Asp
530 535 540
Ala Met Arg Lys Gln Leu His Tyr Ile Gln Ala Phe Tyr Arg Ala Trp
545 550 555 560
Gly Pro Ile Gln Arg Arg Phe Asn Asp Ala Asp Pro Ala Val Thr His
565 570 575
Pro His Leu Thr Val Ile Tyr Pro Pro Leu Thr Pro Ala Ser Ala Glu
580 585 590
Lys Phe Asn Lys Ile Thr Ser Val Ala Ala Val Ser Lys Arg Pro Thr
595 600 605
Thr Leu Pro Tyr Phe Arg Ala Asp Gly Ser Pro Thr Arg Gly Phe Ala
610 615 620
Asn Phe Ser Thr Gly Gly Ile His Gly Ala Glu Tyr Asn Glu Asp Arg
625 630 635 640
Phe Asp His Asp Gln Asp Leu His Thr Val Ala Thr Arg Glu Phe Phe
645 650 655
Ala Ile Leu Asp Ala Thr Leu Ala Ala Leu Tyr Ala Ala His Gln Ala
660 665 670
Glu Pro Glu Ser Ala Asp Tyr Gln Ile Ala Gln Asp Ala Leu Ala Trp
675 680 685
Ala Lys Asn Val Leu Ser Asp Gln Lys Leu Ile Ala Lys Ser Pro Gln
690 695 700
Leu Tyr Asn Pro Glu Thr Gly Val Thr Tyr Glu Trp Glu Phe Val Ala
705 710 715 720
Gln Ala Ala Trp Trp Ile Arg Asn Lys Pro Val Glu Val Ile Leu Pro
725 730 735
Thr Gly Glu Ser Met Thr Val Lys His Lys Ser Val Leu Ala Ser Ala
740 745 750
Ser Tyr Pro Leu Arg Asp Asn Val Ala Tyr Trp Arg Ser Glu Pro Lys
755 760 765
Thr Pro Gln Leu Phe Pro Val Ala Lys Ser Gly Gly Ser Ser Leu Glu
770 775 780
Lys Lys Tyr Asn Tyr Thr Ser Val Gly Thr Ala Ile His Glu Asp Phe
785 790 795 800
Ser Ser Tyr Tyr Pro Leu Leu Leu Thr Asn Met Ala Ala Phe Thr Asn
805 810 815
Ala Asp Leu Gly Ile Asp Glu Lys Thr Gly Arg Pro Arg Asp Arg Tyr
820 825 830
Arg Glu Ile Tyr Glu Gln Lys Glu Ile Tyr Gly Ala Gln Arg Lys Asp
835 840 845
Pro Ser Ile Asp Glu Glu Thr Lys Gln Arg Leu Gly Ile Leu Arg Glu
850 855 860
Gly Thr Lys Leu Ile Leu Asn Ser Ala Thr Gly Ala Ala Asp Ala Gly
865 870 875 880
His Asp Thr Pro Ile Leu Met Asn Asn Arg Val Ile Ala Met Arg Ile
885 890 895
Ile Gly Gln Leu Phe Ser Trp Arg Ile Gly Gln Ala Gln Ser Leu Ala
900 905 910
Gly Ala Thr Ile Ile Ser Thr Asn Thr Asp Gly Leu Tyr Ser Val Leu
915 920 925
Asp Met Gly Thr Asn Gln Arg Val Leu Asp Glu His Ala Thr Ala Ile
930 935 940
Gly Val Gln Ile Glu Pro Glu Glu Leu Asp Ile Val Ser Lys Asp Ser
945 950 955 960
Asn Ser Arg Ala Glu Phe Leu Gly Asn Gly Tyr Ile Asn Ala Ala Gly
965 970 975
Asp Leu Ala Cys Trp Asp Gly Pro Asn Ser Arg Asn Ser Leu Asp His
980 985 990
Pro Ala Phe Val Asp His Val Leu Val Lys Tyr Phe Gln Leu Val Val
995 1000 1005
Asn Asn Thr Val Pro Glu Ile Pro Glu Thr Pro Glu Leu Glu Gly Val
1010 1015 1020
Pro Leu Ala Leu Asp Gln Pro Met Asn Arg His Glu Val Ser Lys Ile
1025 1030 1035 1040
Val Ala Thr Met His Lys Glu Phe Glu Pro Lys Lys Leu Leu Ser Phe
1045 1050 1055
Tyr Gln Asn Ile Leu Ala Ser Ser Arg Gly Ser Asn Thr Phe Leu Phe
1060 1065 1070
Ser Val Pro Tyr Ile Pro Ala Thr Glu Gly Glu Glu Thr His Pro Ala
1075 1080 1085
Thr Asp Thr Ser Thr Ile Ala Thr Pro Thr Leu Ser Phe Asp Ala Tyr
1090 1095 1100
Gly Asn Lys Ala Glu Val Met Pro Thr Gln Ser Thr Val Asp Lys Arg
1105 1110 1115 1120
Val Pro Ser Leu Leu Gln Tyr Tyr Thr Arg Thr Phe His Val Arg Gln
1125 1130 1135
Asp Thr Gln Gln Ala Val Phe Asp Val Ile Gly Ala Asn Pro Val Leu
1140 1145 1150
Ile Ala Ala Ala Lys Ala Ala Ser Ile Ser Pro Ala Ser Ala Asp Ser
1155 1160 1165
Arg Arg Lys Lys Gly Leu Ala Ser Thr Asn Ala Asp Pro Val Ala Lys
1170 1175 1180
His Val Leu Glu Ile Ala Gly Ala Asp Val Glu Ser Leu Arg His Glu
1185 1190 1195 1200
Lys Asp Leu Lys Val Thr Lys His Thr Gly Gln Asp Pro Ala Leu Pro
1205 1210 1215
Val Val Val Phe Asn Gln Thr Ile Trp His Asn Pro Asn Asp Asp Val
1220 1225 1230
Ile Asn Ala Leu Leu Gly Ala Ile Asp Gln Asp Ala Tyr Ile Asp Met
1235 1240 1245
Ala Ile Ser Ser Tyr Asn Lys Ser Trp His Asn Ile Ile Pro Ala
1250 1255 1260
<210>377
<211>487
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(457)
<223>RXA03613
<400>377
gtatttagga attgaaaccc actaaaaaac agactaagtt actaggtgat aacaaaagtt 60
gtttattact tatgggaata gtgtggaaag tagggtgaat atg tca att cct gga 115
Met Ser Ile Pro Gly
1 5
aat gtt agt tat gtt gca cag ggt ggc acc ggt tgg gat ggt gct gat 163
Asn Val Ser Tyr Val Ala Gln Gly Gly Thr Gly Trp Asp Gly Ala Asp
10 15 20
cag tac acc acg ggt gag agt tgg gat cta cag tcg ttc ctt gaa aac 211
Gln Tyr Thr Thr Gly Glu Ser Trp Asp Leu Gln Ser Phe Leu Glu Asn
25 30 35
tcg act gac tac ctc atg att att ggt ggt tcg ctg ctg gca ctt gtt 259
Ser Thr Asp Tyr Leu Met Ile Ile Gly Gly Ser Leu Leu Ala Leu Val
40 45 50
ggt ggt gct gct gtg att tgg ggc ttt gtc aac gtg atg cgc aag ctc 307
Gly Gly Ala Ala Val Ile Trp Gly Phe Val Asn Val Met Arg Lys Leu
55 60 65
ttc ggt ggt cag agt ggt cag cag att cag tgg ttc acc act att ttg 355
Phe Gly Gly Gln Ser Gly Gln Gln Ile Gln Trp Phe Thr Thr Ile Leu
70 75 80 85
Ctc atc att gtc ggt ggt gcg ttc cta gtt ggt ggt atc aca ctt gcg 403
Leu Ile Ile Val Gly Gly Ala Phe Leu Val Gly Gly Ile Thr Leu Ala
90 95 100
ctg aac ttt gcg tct ggt gca ggc gat acg gtt acc ggt ttc ggt aca 451
Leu Asn Phe Ala Ser Gly Ala Gly Asp Thr Val Thr Gly Phe Gly Thr
105 110 115
ggt ggc taagattcag caccgcacaa aaagcacatt 487
Gly Gly
<210>378
<211>119
<212>PRT
<213>谷氨酸棒状杆菌
<400>378
Met Ser Ile Pro Gly Asn Val Ser Tyr Val Ala Gln Gly Gly Thr Gly
1 5 10 15
Trp Asp Gly Ala Asp Gln Tyr Thr Thr Gly Glu Ser Trp Asp Leu Gln
20 25 30
Ser Phe Leu Glu Asn Ser Thr Asp Tyr Leu Met Ile Ile Gly Gly Ser
35 40 45
Leu Leu Ala Leu Val Gly Gly Ala Ala Val Ile Trp Gly Phe Val Asn
50 55 60
Val Met Arg Lys Leu Phe Gly Gly Gln Ser Gly Gln Gln Ile Gln Trp
65 70 75 80
Phe Thr Thr Ile Leu Leu Ile Ile Val Gly Gly Ala Phe Leu Val Gly
85 90 95
Gly Ile Thr Leu Ala Leu Asn Phe Ala Ser Gly Ala Gly Asp Thr Val
100 105 110
Thr Gly Phe Gly Thr Gly Gly
115
<210>379
<211>445
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(415)
<223>RXA03682
<400>379
gtaacggtat accatagaaa caatctgtaa tcctctaagg agcgcgatga aagattcgtc 60
taagtttgcg acaaaaaatt attctgctcg actgttctta ttg ctt tat gtc tta 115
Leu Leu Tyr Val Leu
1 5
acg gtc gta gtc ggg tgg ata ttt gcc ctt ggg aat ccc tat tca gta 163
Thr Val Val Val Gly Trp Ile Phe Ala Leu Gly Asn Pro Tyr Ser Val
10 15 20
gta acc gct ttt tca att ttt gtt gga gtt ggt ttt gct gta gct gca 211
Val Thr Ala Phe Ser Ile Phe Val Gly Val Gly Phe Ala Val Ala Ala
25 30 35
gtt gct tgt gcc cta aca aga aga gct ccc ttg tgg ctt ttg ggg ttc 259
Val Ala Cys Ala Leu Thr Arg Arg Ala Pro Leu Trp Leu Leu Gly Phe
40 45 50
tta ggt ggt gta gga atc gtt ctt gtc ttg atg gga atc tat ttc agg 307
Leu Gly Gly Val Gly Ile Val Leu Val Leu Met Gly Ile Tyr Phe Arg
55 60 65
gta act cag cct gat gga atc cta act gga tat ttg ata ccg att ggt 355
Val Thr Gln Pro Asp Gly Ile Leu Thr Gly Tyr Leu Ile Pro Ile Gly
70 75 80 85
ttt gga ttt ttt ctc gca gaa gca gct gtg gca ata gag cgt tat tgg 403
Phe Gly Phe Phe Leu Ala Glu Ala Ala Val Ala Ile Glu Arg Tyr Trp
90 95 100
cat cgt cgt cac taagcactgc gttcttaaag gaagagcttt 445
His Arg Arg His
105
<210>380
<211>105
<212>PRT
<213>谷氨酸棒状杆菌
<400>380
Leu Leu Tyr Val Leu Thr Val Val Val Gly Trp Ile Phe Ala Leu Gly
1 5 10 15
Asn Pro Tyr Ser Val Val Thr Ala Phe Ser Ile Phe Val Gly Val Gly
20 25 30
Phe Ala Val Ala Ala Val Ala Cys Ala Leu Thr Arg Arg Ala Pro Leu
35 40 45
Trp Leu Leu Gly Phe Leu Gly Gly Val Gly Ile Val Leu Val Leu Met
50 55 60
Gly Ile Tyr Phe Arg Val Thr Gln Pro Asp Gly Ile Leu Thr Gly Tyr
65 70 75 80
Leu Ile Pro Ile Gly Phe Gly Phe Phe Leu Ala Glu Ala Ala Val Ala
85 90 95
Ile Glu Arg Tyr Trp His Arg Arg His
100 105
<210>381
<211>1003
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(973)
<223>RXA03700
<400>381
agcgaatcgg attcagtgat ttgcttgcgg gcgcagaaat gatttttcaa ttagacacac 60
ttagacacac gtaactaaaa cctcagggaa gtgactgata atg gct aat ccg ctc 115
Met Ala Asn Pro Leu
1 5
agc aag ggc tgg aag tat crc atg gca tcg ttc gac aac aag atc gat 163
Ser Lys Gly Trp Lys Tyr Leu Met Ala Ser Phe Asp Asn Lys Ile Asp
10 15 20
gag aat gca gat cca aag atc caa att cag caa gct aca gaa gct gcc 211
Glu Asn Ala Asp Pro Lys Ile Gln Ile Gln Gln Ala Thr Glu Ala Ala
25 30 35
cag aag cag cac cag cag att atg cag cac gct tct cag att atc ggt 259
Gln Lys Gln His Gln Gln Ile Met Gln His Ala Ser Gln Ile Ile Gly
40 45 50
cag cag aag cag ctt gag atg aaa ttg aac cgc ttg gtt act gac cgc 307
Gln Gln Lys Gln Leu Glu Met Lys Leu Asn Arg Leu Val Thr Asp Arg
55 60 65
gat aag ttg cag gaa cag gct cgt cag gca att cag ttg gca gat aag 355
Asp Lys Leu Gln Glu Gln Ala Arg Gln Ala Ile Gln Leu Ala Asp Lys
70 75 80 85
tcc gca aac gaa ggc gac agt gtt aag gct cag gag ttc aac aac act 403
Ser Ala Asn Glu Gly Asp Ser Val Lys Ala Gln Glu Phe Asn Asn Thr
90 95 100
gct gag gtt ttt gct tcc cag ttg gta gct gtg gaa cag cag ttg gag 451
Ala Glu Val Phe Ala Ser Gln Leu Val Ala Val Glu Gln Gln Leu Glu
105 110 115
cag act act gcg ctt cat cag cag gct gag gtt gcg gcg aag gat gct 499
Gln Thr Thr Ala Leu His Gln Gln Ala Glu Val Ala Ala Lys Asp Ala
120 125 130
gtt gcg aag tct aaa gag tct gag atg cgc ctg aaa gag cag atg tct 547
Val Ala Lys Ser Lys Glu Ser Glu Met Arg Leu Lys Glu Gln Met Ser
135 140 145
cag att gat gct cta cgt gcg cag gct gat cag gcg aag atg cag gaa 595
Gln Ile Asp Ala Leu Arg Ala Gln Ala Asp Gln Ala Lys Met Gln Glu
150 155 160 165
agt gtc act aag tct atg gat tct ttg aat cag ttt ggc act cag gat 643
Ser Val Thr Lys Ser Met Asp Ser Leu Asn Gln Phe Gly Thr Gln Asp
170 175 180
tct tct gtt cct acc ctt gat gcg gtg cgt gag aag atc gag cgt cga 691
Ser Ser Val Pro Thr Leu Asp Ala Val Arg Glu Lys Ile Glu Arg Arg
185 190 195
tac gca gat gct ttg ggc gcg cag gaa ctt acc cag aac act gtt agt 739
Tyr Ala Asp Ala Leu Gly Ala Gln Glu Leu Thr Gln Asn Thr Val Ser
200 205 210
gat cgc atg gct gag att gcg cag tcc ggc acc gat atg cgg gcg tca 787
Asp Arg Met Ala Glu Ile Ala Gln Ser Gly Thr Asp Met Arg Ala Ser
215 220 225
gct cgt ttg gct gaa ctt cgc gcg gag gcg ctg ggc acg tcc gca acg 835
Ala Arg Leu Ala Glu Leu Arg Ala Glu Ala Leu Gly Thr Ser Ala Thr
230 235 240 245
cct aag ggc cag cta gag gca ggt gtc gag gat gcg gaa gaa ctt atc 883
Pro Lys Gly Gln Leu Glu Ala Gly Val Glu Asp Ala Glu Glu Leu Ile
250 255 260
gac gaa acc tcc acc cct tca gct acc cca gaa acc gca agc cca gaa 931
Asp Glu Thr Ser Thr Pro Ser Ala Thr Pro Glu Thr Ala Ser Pro Glu
265 270 275
gct gat gct cca gaa gca tcc gca gac gag tcc gag aag aaa 973
Ala Asp Ala Pro Glu Ala Ser Ala Asp Glu Ser Glu Lys Lys
280 285 290
taacctaaac acaaaaaact ggggctaact 1003
<210>382
<211>291
<212>PRT
<213>谷氨酸棒状杆菌
<400>382
Met Ala Asn Pro Leu Ser Lys Gly Trp Lys Tyr Leu Met Ala Ser Phe
1 5 10 15
Asp Asn Lys Ile Asp Glu Asn Ala Asp Pro Lys Ile Gln Ile Gln Gln
20 25 30
Ala Thr Glu Ala Ala Gln Lys Gln His Gln Gln Ile Met Gln His Ala
35 40 45
Ser Gln Ile Ile Gly Gln Gln Lys Gln Leu Glu Met Lys Leu Asn Arg
50 55 60
Leu Val Thr Asp Arg Asp Lys Leu Gln Glu Gln Ala Arg Gln Ala Ile
65 70 75 80
Gln Leu Ala Asp Lys Ser Ala Asn Glu Gly Asp Ser Val Lys Ala Gln
85 90 95
Glu Phe Asn Asn Thr Ala Glu Val Phe Ala Ser Gln Leu Val Ala Val
100 105 110
Glu Gln Gln Leu Glu Gln Thr Thr Ala Leu His Gln Gln Ala Glu Val
115 120 125
Ala Ala Lys Asp Ala Val Ala Lys Ser Lys Glu Ser Glu Met Arg Leu
130 135 140
Lys Glu Gln Met Ser Gln Ile Asp Ala Leu Arg Ala Gln Ala Asp Gln
145 150 155 160
Ala Lys Met Gln Glu Ser Val Thr Lys Ser Met Asp Ser Leu Asn Gln
165 170 175
Phe Gly Thr Gln Asp Ser Ser Val Pro Thr Leu Asp Ala Val Arg Glu
180 185 190
Lys Ile Glu Arg Arg Tyr Ala Asp Ala Leu Gly Ala Gln Glu Leu Thr
195 200 205
Gln Asn Thr Val Ser Asp Arg Met Ala Glu Ile Ala Gln Ser Gly Thr
210 215 220
Asp Met Arg Ala Ser Ala Arg Leu Ala Glu Leu Arg Ala Glu Ala Leu
225 230 235 240
Gly Thr Ser Ala Thr Pro Lys Gly Gln Leu Glu Ala Gly Val Glu Asp
245 250 255
Ala Glu Glu Leu Ile Asp Glu Thr Ser Thr Pro Ser Ala Thr Pro Glu
260 265 270
Thr Ala Ser Pro Glu Ala Asp Ala Pro Glu Ala Ser Ala Asp Glu Ser
275 280 285
Glu Lys Lys
290
<210>383
<211>406
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(376)
<223>RXA03803
<400>383
cggcaaaatg atggccaaaa ccaggaaatc ttggtgtcga aagtacaacg aggttaaggg 60
aattaacccg agggcccaga tctaacggtg agctgccaac atg agg ggt ggt cga 115
Met Arg Gly Gly Arg
1 5
agc act tcg acc ctg gag act ggg caa aac cag ccc aca ctg ccc acg 163
Ser Thr Ser Thr Leu Glu Thr Gly Gln Asn Gln Pro Thr Leu Pro Thr
10 15 20
gac ctc gaa aag agg acc cgt ggg cat tcc tgt ctg gtt tac agg cct 211
Asp Leu Glu Lys Arg Thr Arg Gly His Ser Cys Leu Val Tyr Arg Pro
25 30 35
caa aac cgg tca cag ctg ccc acg aaa ctc gat ttc gag atc cgt ggg 259
Gln Asn Arg Ser Gln Leu Pro Thr Lys Leu Asp Phe Glu Ile Arg Gly
40 45 50
cac tct cgt ttg gtt gct gta gca acc tgc ctc aaa gcc cgg aaa ttt 307
His Ser Arg Leu Val Ala Val Ala Thr Cys Leu Lys Ala Arg Lys Phe
55 60 65
agc ccg ctc aga atc gct tct cag gcc ttg aat gca cga atc cct cac 355
Ser Pro Leu Arg Ile Ala Ser Gln Ala Leu Asn Ala Arg Ile Pro His
70 75 80 85
atg tta ccc atc ggg gat ttc taggcccgca aatcggcaca cagagcatca 406
Met Leu Pro Ile Gly Asp Phe
90
<210>384
<211>92
<212>PRT
<213>谷氨酸棒状杆菌
<400>384
Met Arg Gly Gly Arg Ser Thr Ser Thr Leu Glu Thr Gly Gln Asn Gln
1 5 10 15
Pro Thr Leu Pro Thr Asp Leu Glu Lys Arg Thr Arg Gly His Ser Cys
20 25 30
Leu Val Tyr Arg Pro Gln Asn Arg Ser Gln Leu Pro Thr Lys Leu Asp
35 40 45
Phe Glu Ile Arg Gly His Ser Arg Leu Val Ala Val Ala Thr Cys Leu
50 55 60
Lys Ala Arg Lys Phe Ser Pro Leu Arg Ile Ala Ser Gln Ala Leu Asn
65 70 75 80
Ala Arg Ile Pro His Met Leu Pro Ile Gly Asp Phe
85 90
<210>385
<211>337
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(307)
<223>RXA03823
<400>385
aagcctggtt acgctgatgg tactgcactc gggagggtgt gggagagtag gttaccgccg 60
accaaaaact taaaatagta tggataagcc cagaaacact gtg tgt ttc tgg gtt 115
Val Cys Phe Trp Val
1 5
tat tcg tgt ttg agg ggg tgt ggt gga tac aca tgg ttg tgt gtt cac 163
Tyr Ser Cys Leu Arg Gly Cys Gly Gly Tyr Thr Trp Leu Cys Val His
10 15 20
tac acc ctc tct ttt ttg tgt ttg tgg ggg tct tgg gcc cct tgt gca 211
Tyr Thr Leu Ser Phe Leu Cys Leu Trp Gly Ser Trp Ala Pro Cys Ala
25 30 35
cgt ggc acc tgc gcc tgg ggg caa gcg tcg aca agc aaa aag ctc gta 259
Arg Gly Thr Cys Ala Trp Gly Gln Ala Ser Thr Ser Lys Lys Leu Val
40 45 50
cct tct tta ttt gag gag gta cga gct tta tct gtc ctg gtg aag ttt 307
Pro Ser Leu Phe Glu Glu Val Arg Ala Leu Ser Val Leu Val Lys Phe
55 60 65
tagattttta cagcgatagc tggctcagga 337
<210>386
<211>69
<212>PRT
<213>谷氨酸棒状杆菌
<400>386
Val Cys Phe Trp Val Tyr Ser Cys Leu Arg Gly Cys Gly Gly Tyr Thr
1 5 10 15
Trp Leu Cys Val His Tyr Thr Leu Ser Phe Leu Cys Leu Trp Gly Ser
20 25 30
Trp Ala Pro Cys Ala Arg Gly Thr Cys Ala Trp Gly Gln Ala Ser Thr
35 40 45
Ser Lys Lys Leu Val Pro Ser Leu Phe Glu Glu Val Arg Ala Leu Ser
50 55 60
Val Leu Val Lys Phe
65
<210>387
<211>1564
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1534)
<223>RXA03886
<400>387
caccatccag atcgctgagc tgctggttaa gtaaaaaccc gccattaaaa actccgcttg 60
agtgctacac tttaagcggg gttttaatgt ttgaggggcg atg ggg gtc gag ctt 115
Met Gly Val Glu Leu
1 5
gtg aag tgg aat ttt cca caa gtt tta agt ttc ttt agc agg gga aac 163
Val Lys Trp Asn Phe Pro Gln Val Leu Ser Phe Phe Ser Arg Gly Asn
10 15 20
act gct gat agc act agc gat aaa gaa cat gaa aat gca acg gag cta 211
Thr Ala Asp Ser Thr Ser Asp Lys Glu His Glu Asn Ala Thr Glu Leu
25 30 35
gcg gcc gaa gct tta gcg gat gtc att ttt cag tgg aaa aac tgg gtc 259
Ala Ala Glu Ala Leu Ala Asp Val Ile Phe Gln Trp Lys Asn Trp Val
40 45 50
tac cga cgc gtt gat agt gtg cat cca tcc gaa gga gct cga ggg aga 307
Tyr Arg Arg Val Asp Ser Val His Pro Ser Glu Gly Ala Arg Gly Arg
55 60 65
ctc aga cac tcc atg gat tgc gaa cct cca cct gac cca cgg ctt gcg 355
Leu Arg His Ser Met Asp Cys Glu Pro Pro Pro Asp Pro Arg Leu Ala
70 75 80 85
tac aac gaa gtt gaa cgc cgt tat aac gat att aat gac gta aag gga 403
Tyr Asn Glu Val Glu Arg Arg Tyr Asn Asp Ile Asn Asp Val Lys Gly
90 95 100
caa cta atg gtc ccg cta gcg ttc att gaa aaa ggc ccg atg cgt cat 451
Gln Leu Met Val Pro Leu Ala Phe Ile Glu Lys Gly Pro Met Arg His
105 110 115
ctt gat gtc tgg aga aat gat ggg acg agc att cct gtc ctt ggt tcg 499
Leu Asp Val Trp Arg Asn Asp Gly Thr Ser Ile Pro Val Leu Gly Ser
120 125 130
gaa gaa act ggt gaa cta atg gtg gct gtc ctc atg ctg ttt ttg aag 547
Glu Glu Thr Gly Glu Leu Met Val Ala Val Leu Met Leu Phe Leu Lys
135 140 145
aaa aat ggc atc gag cga aca tct gaa ctt gaa gat gcg atg cgt gcc 595
Lys Asn Gly Ile Glu Arg Thr Ser Glu Leu Glu Asp Ala Met Arg Ala
150 155 160 165
ctt gtg ata tca aga gaa aag gga aac cta gca aaa gca acg aac cta 643
Leu Val Ile Ser Arg Glu Lys Gly Asn Leu Ala Lys Ala Thr Asn Leu
170 175 180
att gaa agt ggg gtg tgg gag ggc aaa aca tta tgg gat agt aat gat 691
Ile Glu Ser Gly Val Trp Glu Gly Lys Thr Leu Trp Asp Ser Asn Asp
185 190 195
cct gca gtg aga ttt gat gtg gga aca aaa gac ttg ctt ttg aat cta 739
Pro Ala Val Arg Phe Asp Val Gly Thr Lys Asp Leu Leu Leu Asn Leu
200 205 210
tct act gat ttt ttg ttg atc ggt ttg ttg ccc gcg gaa act tct gga 787
Ser Thr Asp Phe Leu Leu Ile Gly Leu Leu Pro Ala Glu Thr Ser Gly
215 220 225
atc agg caa gtt tta aaa ttt tca tat cac tgg gtg gtt gaa tta cct 835
Ile Arg Gln Val Leu Lys Phe Ser Tyr His Trp Val Val Glu Leu Pro
230 235 240 245
gac aga ggg ctt ctt aga tca ttg tgg ggt agc gtg tgt gtc gca ttt 883
Asp Arg Gly Leu Leu Arg Ser Leu Trp Gly Ser Val Cys Val Ala Phe
250 255 260
cga gca gct cca atg gaa att gaa tta cca aca cat atg ccc acg gat 931
Arg Ala Ala Pro Met Glu Ile Glu Leu Pro Thr His Met Pro Thr Asp
265 270 275
gcg aaa tcg tac cat ctt gaa ttc cac aca ccg cct gag tta gat act 979
Ala Lys Ser Tyr His Leu Glu Phe His Thr Pro Pro Glu Leu Asp Thr
280 285 290
caa gag tta tcg ctt cca gtt gat tcc aag aat aag ggc cat cgg gtc 1027
Gln Glu Leu Ser Leu Pro Val Asp Ser Lys Asn Lys Gly His Arg Val
295 300 305
ggc tat cca act gac tct agt agg aca cct atc gcc cat gtg cac tcg 1075
Gly Tyr Pro Thr Asp Ser Ser Arg Thr Pro Ile Ala His Val His Ser
310 315 320 325
cat ttt tcc gag ata atg acc aca aat gca aaa gta aaa ttg gag gtt 1123
His Phe Ser Glu Ile Met Thr Thr Asn Ala Lys Val Lys Leu Glu Val
330 335 340
ccc aaa agg gga att tgg gtt act gca ctt tta tcg aat gca cta aca 1171
Pro Lys Arg Gly Ile Trp Val Thr Ala Leu Leu Ser Asn Ala Leu Thr
345 350 355
gcc gga att ttt atc cta gcc ttg ttg cta ccc aat gca act gat gct 1219
Ala Gly Ile Phe Ile Leu Ala Leu Leu Leu Pro Asn Ala Thr Asp Ala
360 365 370
ttg cgt agt gtt gga gga aat gca gcg gcc ttg atg ctt gca gcg ccg 1267
Leu Arg Ser Val Gly Gly Asn Ala Ala Ala Leu Met Leu Ala Ala Pro
375 380 385
gca gta ttt gta agc ttt ctc atc ttt cct aaa gaa cat att ttt gta 1315
Ala Val Phe Val Ser Phe Leu Ile Phe Pro Lys Glu His Ile Phe Val
390 395 400 405
tct tcc atg ctt aat ccg ctt cgt atc gtc gta gga ata tgc gca cta 1363
Ser Ser Met Leu Asn Pro Leu Arg Ile Val Val Gly Ile Cys Ala Leu
410 415 420
ctg ctc ttt atg atg gca gga agt ctt gtg ggt ggg ttg gag cga cca 1411
Leu Leu Phe Met Met Ala Gly Ser Leu Val Gly Gly Leu Glu Arg Pro
425 430 435
ttt ctt att ctt ctg tgg tca ttt gga gct ggg ttt tca ttg cta gtt 1459
Phe Leu Ile Leu Leu Trp Ser Phe Gly Ala Gly Phe Ser Leu Leu Val
440 445 450
gtt agc gca cta gta cta ggt cca ctc cta gga aag ttt ttt gag agg 1507
Val Ser Ala Leu Val Leu Gly Pro Leu Leu Gly Lys Phe Phe Glu Arg
455 460 465
ttg cac ctg cgc cct agt act aag aac tgatgtagag tttgtggaaa 1554
Leu His Leu Arg Pro Ser Thr Lys Asn
470 475
gatgagttag 1564
<210>388
<211>478
<212>PRT
<213>谷氨酸棒状杆菌
<400>388
Met Gly Val Glu Leu Val Lys Trp Asn Phe Pro Gln Val Leu Ser Phe
1 5 10 15
Phe Ser Arg Gly Asn Thr Ala Asp Ser Thr Ser Asp Lys Glu His Glu
20 25 30
Asn Ala Thr Glu Leu Ala Ala Glu Ala Leu Ala Asp Val Ile Phe Gln
35 40 45
Trp Lys Asn Trp Val Tyr Arg Arg Val Asp Ser Val His Pro Ser Glu
50 55 60
Gly Ala Arg Gly Arg Leu Arg His Ser Met Asp Cys Glu Pro Pro Pro
65 70 75 80
Asp Pro Arg Leu Ala Tyr Asn Glu Val Glu Arg Arg Tyr Asn Asp Ile
85 90 95
Asn Asp Val Lys Gly Gln Leu Met Val Pro Leu Ala Phe Ile Glu Lys
100 105 110
Gly Pro Met Arg His Leu Asp Val Trp Arg Asn Asp Gly Thr Ser Ile
115 120 125
Pro Val Leu Gly Ser Glu Glu Thr Gly Glu Leu Met Val Ala Val Leu
130 135 140
Met Leu Phe Leu Lys Lys Asn Gly Ile Glu Arg Thr Ser Glu Leu Glu
145 150 155 160
Asp Ala Met Arg Ala Leu Val Ile Ser Arg Glu Lys Gly Asn Leu Ala
165 170 175
Lys Ala Thr Asn Leu Ile Glu Ser Gly Val Trp Glu Gly Lys Thr Leu
180 185 190
Trp Asp Ser Asn Asp Pro Ala Val Arg Phe Asp Val Gly Thr Lys Asp
195 200 205
Leu Leu Leu Asn Leu Ser Thr Asp Phe Leu Leu Ile Gly Leu Leu Pro
210 215 220
Ala Glu Thr Ser Gly Ile Arg Gln Val Leu Lys Phe Ser Tyr His Trp
225 230 235 240
Val Val Glu Leu Pro Asp Arg Gly Leu Leu Arg Ser Leu Trp Gly Ser
245 250 255
Val Cys Val Ala Phe Arg Ala Ala Pro Met Glu Ile Glu Leu Pro Thr
260 265 270
His Met Pro Thr Asp Ala Lys Ser Tyr His Leu Glu Phe His Thr Pro
275 280 285
Pro Glu Leu Asp Thr Gln Glu Leu Ser Leu Pro Val Asp Ser Lys Asn
290 295 300
Lys Gly His Arg Val Gly Tyr Pro Thr Asp Ser Ser Arg Thr Pro Ile
305 310 315 320
Ala His Val His Ser His Phe Ser Glu Ile Met Thr Thr Asn Ala Lys
325 330 335
Val Lys Leu Glu Val Pro Lys Arg Gly Ile Trp Val Thr Ala Leu Leu
340 345 350
Ser Asn Ala Leu Thr Ala Gly Ile Phe Ile Leu Ala Leu Leu Leu Pro
355 360 365
Asn Ala Thr Asp Ala Leu Arg Ser Val Gly Gly Asn Ala Ala Ala Leu
370 375 380
Met Leu Ala Ala Pro Ala Val Phe Val Ser Phe Leu Ile Phe Pro Lys
385 390 395 400
Glu His Ile Phe Val Ser Ser Met Leu Asn Pro Leu Arg Ile Val Val
405 410 415
Gly Ile Cys Ala Leu Leu Leu Phe Met Met Ala Gly Ser Leu Val Gly
420 425 430
Gly Leu Glu Arg Pro Phe Leu Ile Leu Leu Trp Ser Phe Gly Ala Gly
435 440 445
Phe Ser Leu Leu Val Val Ser Ala Leu Val Leu Gly Pro Leu Leu Gly
450 455 460
Lys Phe Phe Glu Arg Leu His Leu Arg Pro Ser Thr Lys Asn
465 470 475
<210>389
<211>484
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(454)
<223>RXA03940
<400>389
aactcgaccg cttaaacggg cttctagagt gttgatgctt taaggggccg taaatgtagg 60
gttctttgga tgggagctaa ggccagggct cacttttgct ttg atc ggg agg ctc 115
Leu Ile Gly Arg Leu
1 5
caa gcc agc aaa cca gac aca cgt gcc cgc ggt tcg ggt ttt tcg agt 163
Gln Ala Ser Lys Pro Asp Thr Arg Ala Arg Gly Ser Gly Phe Ser Ser
10 15 20
tgg tgg gca ctc tgg ttt ggt tta tcg ctt tcg aaa ccg gtc cca gtt 211
Trp Trp Ala Leu Trp Phe Gly Leu Ser Leu Ser Lys Pro Val Pro Val
25 30 35
gcc caa gtg ttt cga ttt gag gat tcg tgg gca cgc ctg tct ggt tgg 259
Ala Gln Val Phe Arg Phe Glu Asp Ser Trp Ala Arg Leu Ser Gly Trp
40 45 50
cca gaa aaa ctg gcc tta gac cca aaa aac cag act cgc tgt atg gct 307
Pro Glu Lys Leu Ala Leu Asp Pro Lys Asn Gln Thr Arg Cys Met Ala
55 60 65
gtt aga cgc gct tcc gtg act cac ttt agg gga acg ttc ctt cga aaa 355
Val Arg Arg Ala Ser Val Thr His Phe Arg Gly Thr Phe Leu Arg Lys
70 75 80 85
act cga ccc ttt aaa cgg gct tct aga gcc ttg gaa cca ttt gga ctt 403
Thr Arg Pro Phe Lys Arg Ala Ser Arg Ala Leu Glu Pro Phe Gly Leu
90 95 100
ctt cgg agt cta acc aga gta tgg aca aca ata att cct acc tct ggc 451
Leu Arg Ser Leu Thr Arg Val Trp Thr Thr Ile Ile Pro Thr Ser Gly
105 110 115
cag taacctacga cgaagtctcc catgaattaa 484
Gln
<210>390
<211>118
<212>PRT
<213>谷氨酸棒状杆菌
<400>390
Leu Ile Gly Arg Leu Gln Ala Ser Lys Pro Asp Thr Arg Ala Arg Gly
1 5 10 15
Ser Gly Phe Ser Ser Trp Trp Ala Leu Trp Phe Gly Leu Ser Leu Ser
20 25 30
Lys Pro Val Pro Val Ala Gln Val Phe Arg Phe Glu Asp Ser Trp Ala
35 40 45
Arg Leu Ser Gly Trp Pro Glu Lys Leu Ala Leu Asp Pro Lys Asn Gln
50 55 60
Thr Arg Cys Met Ala Val Arg Arg Ala Ser Val Thr His Phe Arg Gly
65 70 75 80
Thr Phe Leu Arg Lys Thr Arg Pro Phe Lys Arg Ala Ser Arg Ala Leu
85 90 95
Glu Pro Phe Gly Leu Leu Arg Ser Leu Thr Arg Val Trp Thr Thr Ile
100 105 110
Ile Pro Thr Ser Gly Gln
115
<210>391
<211>418
<212>DNA
<213>谷酸棒状杆菌
<220>
<221>CDS
<222>(101)..(388)
<223>RXA03947
<400>391
acgcccccaa atacctgcga gtcacataag tattcccaaa caattcactt taaggggtca 60
acatccccaa aataggtcca catatggatg aggcccgttt atg gcc tta aaa acg 115
Met Ala Leu Lys Thr
1 5
att ctg agg cac cta cgg ttt gtt gcg aat gag aca ggt gtg att cct 163
Ile Leu Arg His Leu Arg Phe Val Ala Asn Glu Thr Gly Val Ile Pro
10 15 20
gca ggt tcc ctg atc aag gat tca cca caa tcc tgt ggc cag acc tta 211
Ala Gly Ser Leu Ile Lys Asp Ser Pro Gln Ser Cys Gly Gln Thr Leu
25 30 35
aaa tcg aac aga ttt ttg cac cac agg ccg tgg aag ttg caa aaa tcc 259
Lys Ser Asn Arg Phe Leu His His Arg Pro Trp Lys Leu Gln Lys Ser
40 45 50
acc aca aac cgc ccc cta atc agc cat ttg tgg tgg ata ttc gca cat 307
Thr Thr Asn Arg Pro Leu Ile Ser His Leu Trp Trp Ile Phe Ala His
55 60 65
tct ttc gct acg ccc tca ata tgg aaa ttt cgg gcc cta att agc tgc 355
Ser Phe Ala Thr Pro Ser Ile Trp Lys Phe Arg Ala Leu Ile Ser Cys
70 75 80 85
ggt cgc cga gca aat tgg cgg ctc cgg cgt cga taagctgttg tgcaacgagc 408
Gly Arg Arg Ala Asn Trp Arg Leu Arg Arg Arg
90 95
tcgccgagct 418
<210>392
<211>96
<212>PRT
<213>谷氨酸棒状杆菌
<400>392
Met Ala Leu Lys Thr Ile Leu Arg His Leu Arg Phe Val Ala Asn Glu
1 5 10 15
Thr Gly Val Ile Pro Ala Gly Ser Leu Ile Lys Asp Ser Pro Gln Ser
20 25 30
Cys Gly Gln Thr Leu Lys Ser Asn Arg Phe Leu His His Arg Pro Trp
35 40 45
Lys Leu Gln Lys Ser Thr Thr Asn Arg Pro Leu Ile Ser His Leu Trp
50 55 60
Trp Ile Phe Ala His Ser Phe Ala Thr Pro Ser Ile Trp Lys Phe Arg
65 70 75 80
Ala Leu Ile Ser Cys Gly Arg Arg Ala Asn Trp Arg Leu Arg Arg Arg
85 90 95
<210>393
<211>712
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(682)
<223>RXA03955
<400>393
agcgtgccgg tgcaaaccaa atcctcacgt actttgccgt tgacgctgcc cgcgcactgc 60
gaaacgctta acaagaacta actgattagt aggactcccc atg agc atg ctt ccc 115
Met Ser Met Leu Pro
1 5
aat ctc aca ccg aag aaa tct gcc ccg cct tca tct tct gct tct gcg 163
Asn Leu Thr Pro Lys Lys Ser Ala Pro Pro Ser Ser Ser Ala Ser Ala
10 15 20
aaa aat gct gga gaa aag aag gaa gac acc gtc aag ctc ctg att cag 211
Lys Asn Ala Gly Glu Lys Lys Glu Asp Thr Val Lys Leu Leu Ile Gln
25 30 35
atg tgg gct gtg atg atc ggt ttg gag ctg ctg cac cag atc tta aac 259
Met Trp Ala Val Met Ile Gly Leu Glu Leu Leu His Gln Ile Leu Asn
40 45 50
gtg gtg atg agc ttt acg gac ccg act gcg ctg cgg act gtt gcc aaa 307
Val Val Met Ser Phe Thr Asp Pro Thr Ala Leu Arg Thr Val Ala Lys
55 60 65
gaa caa gca gca gca cag ggg ctt tcc gat ggc att gtt aac gcc aca 355
Glu Gln Ala Ala Ala Gln Gly Leu Ser Asp Gly Ile Val Asn Ala Thr
70 75 80 85
gtc atc gca gca atc ttg gtc atg gga ttc ttc aac ctc att gtg att 403
Val Ile Ala Ala Ile Leu Val Met Gly Phe Phe Asn Leu Ile Val Ile
90 95 100
gcc att ttg tcc tgg atg gtg gtg atg gtg aaa aae cgt tcc aaa cgc 451
Ala Ile Leu Ser Trp Met Val Val Met Val Lys Asn Arg Ser Lys Arg
105 110 115
cta ccg acg gca ttc ttg ctg ctc acc atc ttc agt ttc ttc ttt atc 499
Leu Pro Thr Ala Phe Leu Leu Leu Thr Ile Phe Ser Phe Phe Phe Ile
120 125 130
cta aga gtc ttg atg ctg ttt ttc gcc tca cct ggt ggg act gat gtt 547
Leu Arg Val Leu Met Leu Phe Phe Ala Ser Pro Gly Gly Thr Asp Val
135 140 145
cca atg gca cta ttt gct atc gac ggt tgt gta caa atc ctc gtc gga 595
Pro Met Ala Leu Phe Ala Ile Asp Gly Cys Val Gln Ile Leu Val Gly
150 155 160 165
gtg agc gcc ggt gtg gca tat gcg ctc tcg cgc act gag cag cct gtc 643
Val Ser Ala Gly Val Ala Tyr Ala Leu Ser Arg Thr Glu Gln Pro Val
170 175 180
aaa atg ctc acc gac tac gca cca gaa gac gct aaa cgg taagtactga 692
Lys Met Leu Thr Asp Tyr Ala Pro Glu Asp Ala Lys Arg
185 190
agaattttta aagacgacgg 712
<210>394
<211>194
<212>PRT
<213>谷氨酸棒状杆菌
<400>394
Met Ser Met Leu Pro Asn Leu Thr Pro Lys Lys Ser Ala Pro Pro Ser
1 5 10 15
Ser Ser Ala Ser Ala Lys Asn Ala Gly Glu Lys Lys Glu Asp Thr Val
20 25 30
Lys Leu Leu Ile Gln Met Trp Ala Val Met Ile Gly Leu Glu Leu Leu
35 40 45
His Gln Ile Leu Asn Val Val Met Ser Phe Thr Asp Pro Thr Ala Leu
50 55 60
Arg Thr Val Ala Lys Glu Gln Ala Ala Ala Gln Gly Leu Ser Asp Gly
65 70 75 80
Ile Val Asn Ala Thr Val Ile Ala Ala Ile Leu Val Met Gly Phe Phe
85 90 95
Asn Leu Ile Val Ile Ala Ile Leu Ser Trp Met Val Val Met Val Lys
100 105 110
Asn Arg Ser Lys Arg Leu Pro Thr Ala Phe Leu Leu Leu Thr Ile Phe
115 120 125
Ser Phe Phe Phe Ile Leu Arg Val Leu Met Leu Phe Phe Ala Ser Pro
130 135 140
Gly Gly Thr Asp Val Pro Met Ala Leu Phe Ala Ile Asp Gly Cys Val
145 150 155 160
Gln Ile Leu Val Gly Val Ser Ala Gly Val Ala Tyr Ala Leu Ser Arg
165 170 175
Thr Glu Gln Pro Val Lys Met Leu Thr Asp Tyr Ala Pro Glu Asp Ala
180 185 190
Lys Arg
<210>395
<211>322
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(292)
<223>RXA03975
<400>395
agacgcatca atcgcttcgt ggtcgtaggc cttgagccta atgcggatct tttgtcccgc 60
cacgcttatc ctcttcctcg ctcgtcccac atcttccatt ttg gaa aag cgg tgg 115
Leu Glu Lys Arg Trp
1 5
gaa ttc gct tca ttc ttt ctc tat aac gtt gtc cgg gct tgg ggc tcg 163
Glu Phe Ala Ser Phe Phe Leu Tyr Asn Val Val Arg Ala Trp Gly Ser
10 15 20
ggc gca acg cca gtg ttt aac ctg acc tgt caa atg ctt ctg aca ttg 211
Gly Ala Thr Pro Val Phe Asn Leu Thr Cys Gln Met Leu Leu Thr Leu
25 30 35
gat cac ggg cgc tgt aca aat aaa acg ctc cac gat gaa ccc aac aat 259
Asp His Gly Arg Cys Thr Asn Lys Thr Leu His Asp Glu Pro Asn Asn
40 45 50
gaa gtc agg gaa atc gga agg gac caa aat gcg tgatctaggc cctaacgtaa 312
Glu Val Arg Glu Ile Gly Arg Asp Gln Asn Ala
55 60
ctgccgctgt 322
<210>396
<211>64
<212>PRT
<213>谷氨酸棒状杆菌
<400>396
Leu Glu Lys Arg Trp Glu Phe Ala Ser Phe Phe Leu Tyr Asn Val Val
1 5 10 15
Arg Ala Trp Gly Ser Gly Ala Thr Pro Val Phe Asn Leu Thr Cys Gln
20 25 30
Met Leu Leu Thr Leu Asp His Gly Arg Cys Thr Asn Lys Thr Leu His
35 40 45
Asp Glu Pro Asn Asn Glu Val Arg Glu Ile Gly Arg Asp Gln Asn Ala
50 55 60
<210>397
<211>604
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(574)
<223>RXA04007
<400>397
ggccggtggg caagatgctt ctggaaatat aatgatgtgc tcaggcggac aatgggtcta 60
tgggccataa atctaaaatc aaagagtgca ctcctgcagc atg ggt ttt ggg aag 115
Met Gly Phe Gly Lys
1 5
gcg ata ctc tta gaa att atg gat ccg atg aac agc tac aac acc atg 163
Ala Ile Leu Leu Glu Ile Met Asp Pro Met Asn Ser Tyr Asn Thr Met
10 15 20
aag tcc cac gtc acc cca att cca acg ccg gtg agc gtg cgg ctg agt 211
Lys Ser His Val Thr Pro Ile Pro Thr Pro Val Ser Val Arg Leu Ser
25 30 35
agc tcg gta tta gta ggt gct gcc atc gct tca tta acc act gat cta 259
Ser Ser Val Leu Val Gly Ala Ala Ile Ala Ser Leu Thr Thr Asp Leu
40 45 50
gcc ccg gcc gta aag ttt tct gtc act gga gtt ggt ctg gca ctc gcg 307
Ala Pro Ala Val Lys Phe Ser Val Thr Gly Val Gly Leu Ala Leu Ala
55 60 65
ctg ctc atc gct ttt gcg cat cct tac cgt ggg gag atg agg atg tac 355
Leu Leu Ile Ala Phe Ala His Pro Tyr Arg Gly Glu Met Arg Met Tyr
70 75 80 85
cgc ttc caa aac aat att tca cca gtc ccc acc att ggc cag gtg atg 403
Arg Phe Gln Asn Asn Ile Ser Pro Val Pro Thr Ile Gly Gln Val Met
90 95 100
cca cta ttt ttc aca tgg ctg gcg ctg atg ctt gca ccg att att tca 451
Pro Leu Phe Phe Thr Trp Leu Ala Leu Met Leu Ala Pro Ile Ile Ser
105 110 115
gga gcg cca ttg tgg gca acg att cta gtg ttc ctg gca gct acc ggc 499
Gly Ala Pro Leu Trp Ala Thr Ile Leu Val Phe Leu Ala Ala Thr Gly
120 125 130
tgg atg tat tta acg ttc ccc cac gtg gat ggc agc cgg aag ctg gcg 547
Trp Met Tyr Leu Thr Phe Pro His Val Asp Gly Ser Arg Lys Leu Ala
135 140 145
ttc gct gaa ggc ccc cgc cga aac acc taaacggacc acccaggaaa 594
Phe Ala Glu Gly Pro Arg Arg Asn Thr
150 155
tcgacctaag 604
<210>398
<211>158
<212>PRT
<213>谷氨酸棒状杆菌
<400>398
Met Gly Phe Gly Lys Ala Ile Leu Leu Glu Ile Met Asp Pro Met Asn
l 5 10 15
Ser Tyr Asn Thr Met Lys Ser His Val Thr Pro Ile Pro Thr Pro Val
20 25 30
Ser Val Arg Leu Ser Ser Ser Val Leu Val Gly Ala Ala Ile Ala Ser
35 40 45
Leu Thr Thr Asp Leu Ala Pro Ala Val Lys Phe Ser Val Thr Gly Val
50 55 60
Gly Leu Ala Leu Ala Leu Leu Ile Ala Phe Ala His Pro Tyr Arg Gly
65 70 75 80
Glu Met Arg Met Tyr Arg Phe Gln Asn Asn Ile Ser Pro Val Pro Thr
85 90 95
Ile Gly Gln Val Met Pro Leu Phe Phe Thr Trp Leu Ala Leu Met Leu
100 105 110
Ala Pro Ile Ile Ser Gly Ala Pro Leu Trp Ala Thr Ile Leu Val Phe
115 120 125
Leu Ala Ala Thr Gly Trp Met Tyr Leu Thr Phe Pro His Val Asp Gly
130 135 140
Ser Arg Lys Leu Ala Phe Ala Glu Gly Pro Arg Arg Asn Thr
145 150 155
<210>399
<211>718
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(688)
<223>RXA04075
<400>399
taagaagtcg agttaaatat tcctgttttt gtattataag caattggact ccctagtatt 60
cacggatggg gagtcaatcg caaaagctag actggcgtct atg act gac atg tgg 115
Met Thr Asp Met Trp
1 5
ttt agc tct gac ctt cat tta ggt cac aaa ttc gtg gca tcg atg cgg 163
Phe Ser Ser Asp Leu His Leu Gly His Lys Phe Val Ala Ser Met Arg
10 15 20
ggt ttc gac gat cca gat gaa cac gat gaa gta ata ctg agc aat ttt 211
Gly Phe Asp Asp Pro Asp Glu His Asp Glu Val Ile Leu Ser Asn Phe
25 30 35
gag aac acg atc ggc cca gat gat gtg ctg tgg att ctg gga gat ctt 259
Glu Asn Thr Ile Gly Pro Asp Asp Val Leu Trp Ile Leu Gly Asp Leu
40 45 50
tcc tct ggt gcc cat cgc gct gaa gag cgc gcg ctg ggc tta att gct 307
Ser Ser Gly Ala His Arg Ala Glu Glu Arg Ala Leu Gly Leu Ile Ala
55 60 65
gag cgg ctg ggc ggc gtc gtc aag cat ctt gtt cct gga aac cac gat 355
Glu Arg Leu Gly Gly Val Val Lys His Leu Val Pro Gly Asn His Asp
70 75 80 85
tct tgc cac ccg atg tac cgg cat gcg tac aag cga cag cgg cgg ttt 403
Ser Cys His Pro Met Tyr Arg His Ala Tyr Lys Arg Gln Arg Arg Phe
90 95 100
ttg gag gtt ttt gat tcg gtt cag gcg ttt cag cgg atg aaa tgg gac 451
Leu Glu Val Phe Asp Ser Val Gln Ala Phe Gln Arg Met Lys Trp Asp
105 110 115
gac gag gat gtc tac ttg tcg cat ttc cct cgg ccg ggc caa gat cat 499
Asp Glu Asp Val Tyr Leu Ser His Phe Pro Arg Pro Gly Gln Asp His
120 125 130
ccg ggg atg gaa tcg cgg ttt gat gat ctc cgc ctg cgt gtg cct ttg 547
Pro Gly Met Glu Ser Arg Phe Asp Asp Leu Arg Leu Arg Val Pro Leu
135 140 145
ctg att cat ggg cat ttg cat tcc cag ttc ccc atg acg ggg ccg ggg 595
Leu Ile His GlV His Leu His Ser Gln Phe Pro Met Thr Gly Pro Gly
150 155 160 165
cag gtt gat gtt ggg gta gag gcg tgg ggt ttg aag ccg gca cct cgt 643
Gln Val Asp Val Gly Val Glu Ala Trp Gly Leu Lys Pro Ala Pro Arg
170 175 180
gaa tta gtg cag ctc aag ctg tgg gaa tcg ctg agc gag aag att 688
Glu Leu Val Gln Leu Lys Leu Trp Glu Ser Leu Ser Glu Lys Ile
185 190 195
taaaagacaa agaaggcgca tcctaaggaa 718
<210>400
<211>196
<212>PRT
<213>谷氨酸棒状杆菌
<400>400
Met Thr Asp Met Trp Phe Ser Ser Asp Leu His Leu Gly His Lys Phe
1 5 10 15
Val Ala Ser Met Arg Gly Phe Asp Asp Pro Asp Glu His Asp Glu Val
20 25 30
Ile Leu Ser Asn Phe Glu Asn Thr Ile Gly Pro Asp Asp Val Leu Trp
35 40 45
Ile Leu Gly Asp Leu Ser Ser Gly Ala His Arg Ala Glu Glu Arg Ala
50 55 60
Leu Gly Leu Ile Ala Glu Arg Leu Gly Gly Val Val Lys His Leu Val
65 70 75 80
Pro Gly Asn His Asp Ser Cys His Pro Met Tyr Arg His Ala Tyr Lys
85 90 95
Arg Gln Arg Arg Phe Leu Glu Val Phe Asp Ser Val Gln Ala Phe Gln
100 105 110
Arg Met Lys Trp Asp Asp Glu Asp Val Tyr Leu Ser His Phe Pro Arg
115 120 125
Pro Gly Gln Asp His Pro Gly Met Glu Ser Arg Phe Asp Asp Leu Arg
130 135 140
Leu Arg Val Pro Leu Leu Ile His Gly His Leu His Ser Gln Phe Pro
145 150 155 160
Met Thr Gly Pro Gly Gln Val Asp Val Gly Val Glu Ala Trp Gly Leu
165 170 175
Lys Pro Ala Pro Arg Glu Leu Val Gln Leu Lys Leu Trp Glu Ser Leu
180 185 190
Ser Glu Lys Ile
195
<210>401
<211>472
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(442)
<223>RXA04085
<400>401
gatctggacg gactcggcgc cgcagtcgtg ggcgagtatt tacgacaccg ctactgagca 60
ttggacgttt tcgccttagc caggccagca agaaccatga gtg att tgt ggg tat 115
Val Ile Cys Gly Tyr
1 5
tgc tgc ctg gct tac cag tcc aca caa caa cac tct gct cag aat cat 163
Cys Cys Leu Ala Tyr Gln Ser Thr Gln Gln His Ser Ala Gln Asn His
10 15 20
agg ggt cta aca aag tgt ccc aat caa cgg tta aac ctg gca ata ttt 211
Arg Gly Leu Thr Lys Cys Pro Asn Gln Arg Leu Asn Leu Ala Ile Phe
25 30 35
gga gtc cat ctc atc ctc ata ggc gtg ctg ctg tgc cta tcc ctt aag 259
Gly Val His Leu Ile Leu Ile Gly Val Leu Leu Cys Leu Ser Leu Lys
40 45 50
gtt cct acg atc atc ggc acc cta gcg gtc atc aca ggc ata gga tat 307
Val Pro Thr Ile Ile Gly Thr Leu Ala Val Ile Thr Gly Ile Gly Tyr
55 60 65
tta gtc gat gca gtg gca gtg ctc cta ggc tcc cca aat cct ggc att 355
Leu Val Asp Ala Val Ala Val Leu Leu Gly Ser Pro Asn Pro Gly Ile
70 75 80 85
tct gag ttc aca ttc gtg ggc gaa gtg gtt ctg ttt atc tgg ctg atc 403
Ser Glu Phe Thr Phe Val Gly Glu Val Val Leu Phe Ile Trp Leu Ile
90 95 100
tac tcc gga gtt acc gtt ccg aag gag gaa agg cca tgc taacgagact 452
Tyr Ser Gly Val Thr Val Pro Lys Glu Glu Arg Pro Cys
105 110
ttaaccgtta ctatgtagga 472
<210>402
<211>114
<212>PRT
<213>谷氨酸棒状杆菌
<400>402
Val Ile Cys Gly Tyr Cys Cys Leu Ala Tyr Gln Ser Thr Gln Gln His
1 5 10 15
Ser Ala Gln Asn His Arg Gly Leu Thr Lys Cys Pro Asn Gln Arg Leu
20 25 30
Asn Leu Ala Ile Phe Gly Val His Leu Ile Leu Ile Gly Val Leu Leu
35 40 45
Cys Leu Ser Leu Lys Val Pro Thr Ile Ile Gly Thr Leu Ala Val Ile
50 55 60
Thr Gly Ile Gly Tyr Leu Val Asp Ala Val Ala Val Leu Leu Gly Ser
65 70 75 80
Pro Asn Pro Gly Ile Ser Glu Phe Thr Phe Val Gly Glu Val Val Leu
85 90 95
Phe Ile Trp Leu Ile Tyr Ser Gly Val Thr Val Pro Lys Glu Glu Arg
100 105 110
Pro Cys
<210>403
<211>349
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(319)
<223>RXA04092
<400>403
ttctattggc caagcaagct cactggggtg agcatggtga caagacaatg caggtagccg 60
atgcagctgc cgaaattaac gatgaagggg aggaacgccg atg atc ttt tca gaa 115
Met Ile Phe Ser Glu
1 5
gag att ctc cat acc gac tgg ttc gca gca ctt gct gct ttc gtc gcg 163
Glu Ile Leu His Thr Asp Trp Phe Ala Ala Leu Ala Ala Phe Val Ala
10 15 20
att aac aca aca atc tat gtg gtt ctt gct att gcc aag acg ctg ccg 211
Ile Asn Thr Thr Ile Tyr Val Val Leu Ala Ile Ala Lys Thr Leu Pro
25 30 35
aag att tat gtc act gac tac tta ccg cgg aat tat gag cgt gct gag 259
Lys Ile Tyr Val Thr Asp Tyr Leu Pro Arg Asn Tyr Glu Arg Ala Glu
40 45 50
aca cgc agc att tat cca gac gtt gaa gaa ccg aaa agg aag aag tct 307
Thr Arg Ser Ile Tyr Pro Asp Val Glu Glu Pro Lys Arg Lys Lys Ser
55 60 65
gag aag aag gat tagggcggat aacgaatgcg cacggcttca 349
Glu Lys Lys Asp
70
<210>404
<211>73
<212>PRT
<213>谷氨酸棒状杆菌
<400>404
Met Ile Phe Ser Glu Glu Ile Leu His Thr Asp Trp Phe Ala Ala Leu
1 5 10 15
Ala Ala Phe Val Ala Ile Asn Thr Thr Ile Tyr Val Val Leu Ala Ile
20 25 30
Ala Lys Thr Leu Pro Lys Ile Tyr Val Thr Asp Tyr Leu Pro Arg Asn
35 40 45
Tyr Glu Arg Ala Glu Thr Arg Ser Ile Tyr Pro Asp Val Glu Glu Pro
50 55 60
Lys Arg Lys Lys Ser Glu Lys Lys Asp
65 70
<210>405
<211>385
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(355)
<223>RXA04095
<400>405
ctgtgcctcc tagcgatgag tagggagttg gattatttac gcttcatcgc tggggtggtc 60
ggacatactt gtgctggcat ccacattact gtgagacctc gtg cct cct ggg ccg 115
Val Pro Pro Gly Pro
1 5
ggt cag gtt cct att agc agt ttg agt atg tca ctg tct tcg gcg gca 163
Gly Gln Val Pro Ile Ser Ser Leu Ser Met Ser Leu Ser Ser Ala Ala
10 15 20
tca ccc ccc gga tca ttt tca gac agg gac agg aat aaa cca tac cga 211
Ser Pro Pro Gly Ser Phe Ser Asp Arg Asp Arg Asn Lys Pro Tyr Arg
25 30 35
agc cag cga cta gtt cca ctg tcc ttt cgg acg gcg gga acg gac ata 259
Ser Gln Arg Leu Val Pro Leu Ser Phe Arg Thr Ala Gly Thr Asp Ile
40 45 50
aac ata gtc cgc cct ctc ggg tgg acg gac tat gaa tct tcg gtg ccc 307
Asn Ile Val Arg Pro Leu Gly Trp Thr Asp Tyr Glu Ser Ser Val Pro
55 60 65
tgg ggt gga act gct aac aac gta atg ggc agg ccc aaa aga ata ctt 355
Trp Gly Gly Thr Ala Asn Asn Val Met Gly Arg Pro Lys Arg Ile Leu
70 75 80 85
taaaaggata agatttatat gacagcctga 385
<210>406
<211>85
<212>PRT
<213>谷氨酸棒状杆菌
<400>406
Val Pro Pro Gly Pro Gly Gln Val Pro Ile Ser Ser Leu Ser Met Ser
1 5 10 15
Leu Ser Ser Ala Ala Ser Pro Pro Gly Ser Phe Ser Asp Arg Asp Arg
20 25 30
Asn Lys Pro Tyr Arg Ser Gln Arg Leu Val Pro Leu Ser Phe Arg Thr
35 40 45
Ala Gly Thr Asp Ile Asn Ile Val Arg Pro Leu Gly Trp Thr Asp Tyr
50 55 60
Glu Ser Ser Val Pro Trp Gly Gly Thr Ala Asn Asn Val Met Gly Arg
65 70 75 80
Pro Lys Arg Ile Leu
85
<210>407
<211>310
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(280)
<223>RXA04101
<400>407
ggcgtccaat gacccgatca gtccttttga tgccaatgag gatgcatcac gcttccctac 60
cgtggcaatc accaatcctg aagctaagga gtcctaaatc atg gga act act gca 115
Met Gly Thr Thr Ala
1 5
atc atc atg atg gtg ttg ttc atg gtc atc att tgg ggt ggc ctg gtt 163
Ile Ile Met Met Val Leu Phe Met Val Ile Ile Trp Gly Gly Leu Val
10 15 20
tat gcc acc atc gca ctt cga cgc gaa cca gat gag aag gtt gga ctg 211
Tyr Ala Thr Ile Ala Leu Arg Arg Glu Pro Asp Glu Lys Val Gly Leu
25 30 35
ttt gga acc tct cca tat gcc acc gac tct gtt ctt att gag caa gag 259
Phe Gly Thr Ser Pro Tyr Ala Thr Asp Ser Val Leu Ile Glu Gln Glu
40 45 50
tct gaa cga cct gca acg gcc taaagatctc tgtaggaaac actcccgcca 310
Ser Glu Arg Pro Ala Thr Ala
55 60
<210>408
<211>60
<212>PRT
<213>谷氨酸棒状杆菌
<400>408
Met Gly Thr Thr Ala Ile Ile Met Met Val Leu Phe Met Val Ile Ile
1 5 10 15
Trp Gly Gly Leu Val Tyr Ala Thr Ile Ala Leu Arg Arg Glu Pro Asp
20 25 30
Glu Lys Val Gly Leu Phe Gly Thr Ser Pro Tyr Ala Thr Asp Ser Val
35 40 45
Leu Ile Glu Gln Glu Ser Glu Arg Pro Ala Thr Ala
50 55 60
<210>409
<211>382
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(352)
<223>RXA04169
<400>409
tcccagggtg gttgaccact caagcatgca agcgtcacaa atattccgga cattctgact 60
aatttcccta gagctgtaga cagcagtcct gatgaatcaa atg aaa cgc ctt atc 115
Met Lys Arg Leu Ile
1 5
tct gtc gtt ggg act aca ttt atc ttt gca act age tgc tca gca acc 163
Ser Val Val Gly Thr Thr Phe Ile Phe Ala Thr Ser Cys Ser Ala Thr
10 15 20
gat tcc gag gtt gat tcg cca gct ccg aca agt gga gtt aca gtc tct 211
Asp Ser Glu Val Asp Ser Pro Ala Pro Thr Ser Gly Val Thr Val Ser
25 30 35
tca att atc aga tct ttt gaa ccc aat aga aaa gaa gaa ctc ctt aac 259
Ser Ile Ile Arg Ser Phe Glu Pro Asn Arg Lys Glu Glu Leu Leu Asn
40 45 50
aag ctc gta agg ttg gct cct gta aac caa gtc aat cca cgc aga gct 307
Lys Leu Val Arg Leu Ala Pro Val Asn Gln Val Asn Pro Arg Arg Ala
55 60 65
tcc cgt atc agg aaa tta cga ttt ccc aac tcc agt cga cct agg 352
Ser Arg Ile Arg Lys Leu Arg Phe Pro Asn Ser Ser Arg Pro Arg
70 75 80
tgagccagat tccgctgaga tctacacaga 382
<210>410
<211>84
<212>PRT
<213>谷氨酸棒状杆菌
<400>410
Met Lys Arg Leu Ile Ser Val Val Gly Thr Thr Phe Ile Phe Ala Thr
1 5 10 15
Ser Cys Ser Ala Thr Asp Ser Glu Val Asp Ser Pro Ala Pro Thr Ser
20 25 30
Gly Val Thr Val Ser Ser Ile Ile Arg Ser Phe Glu Pro Asn Arg Lys
35 40 45
Glu Glu Leu Leu Asn Lys Leu Val Arg Leu Ala Pro Val Asn Gln Val
50 55 60
Asn Pro Arg Arg Ala Ser Arg Ile Arg Lys Leu Arg Phe Pro Asn Ser
65 70 75 80
Ser Arg Pro Arg
<210>411
<211>451
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(421)
<223>RXA04172
<400>411
catgatggtt tgggaaacgt tgtgaatatt gacatctagc tcagccaacg cggtggaaac 60
tgcggcgata attccggtgt ggtcctgccc tgtgacagtc atg att gca aac atg 115
Met Ile Ala Asn Met
1 5
ggt tca tgc tac aac gag cgg tgg aca cga cac ttt ggg tct atc att 163
Gly Ser Cys Tyr Asn Glu Arg Trp Thr Arg His Phe Gly Ser Ile Ile
10 15 20
tgg ggt atg cca act cta ttt aat cgc tcg tcc aag aca tat aaa tca 211
Trp Gly Met Pro Thr Leu Phe Asn Arg Ser Ser Lys Thr Tyr Lys Ser
25 30 35
ttg cac gct gat tgg ttg gat ttg ccc act gaa ctg cgt ttt cca gtc 259
Leu His Ala Asp Trp Leu Asp Leu Pro Thr Glu Leu Arg Phe Pro Val
40 45 50
att gct gtc gcg gtc gca gaa ttt gcc acc aaa gtg tcc gtg tgg gtg 307
Ile Ala Val Ala Val Ala Glu Phe Ala Thr Lys Val Ser Val Trp Val
55 60 65
tcg ctg agt cgg cgc gca cct gaa aaa gtc cgc ggc ccc aaa tgg gta 355
Ser Leu Ser Arg Arg Ala Pro Glu Lys Val Arg Gly Pro Lys Trp Val
70 75 80 85
tgg gcg ctt ttg acc atc gtc aat ggt gtt ggc ccc gct gcc tac tgg 403
Trp Ala Leu Leu Thr Ile Val Asn Gly Val Gly Pro Ala Ala Tyr Trp
90 95 100
gct ttc ggc agg aaa aac taagagttgt tagggtggcg ctcatgacta 451
Ala Phe Gly Arg Lys Asn
105
<210>412
<211>107
<212>PRT
<213>谷氨酸棒状杆菌
<400>412
Met Ile Ala Asn Met Gly Ser Cys Tyr Asn Glu Arg Trp Thr Arg His
1 5 10 15
Phe Gly Ser Ile Ile Trp Gly Met Pro Thr Leu Phe Asn Arg Ser Ser
20 25 30
Lys Thr Tyr Lys Ser Leu His Ala Asp Trp Leu Asp Leu Pro Thr Glu
35 40 45
Leu Arg Phe Pro Val Ile Ala Val Ala Val Ala Glu Phe Ala Thr Lys
50 55 60
Val Ser Val Trp Val Ser Leu Ser Arg Arg Ala Pro Glu Lys Val Arg
65 70 75 80
Gly Pro Lys Trp Val Trp Ala Leu Leu Thr Ile Val Asn Gly Val Gly
85 90 95
Pro Ala Ala Tyr Trp Ala Phe Gly Arg Lys Asn
100 105
<210>413
<211>481
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(451)
<223>RXA04181
<400>413
tggtttcgtc gatgaatact gttgttactg cgtgagctgg tgatgagcca gaatcaggtg 60
actggaatgc ctgtggttca cgcttagggg atgattcaca atg aag aag cca act 115
Met Lys Lys Pro Thr
1 5
cta tat tta aat ctc gaa tat gga atc cga aac tcg ctg tgg gcg gac 163
Leu Tyr Leu Asn Leu Glu Tyr Gly Ile Arg Asn Ser Leu Trp Ala Asp
10 15 20
aag gtg cca ttt ccg agg aaa ggg gta cgg ttt gtg acg tat cct gat 211
Lys Val Pro Phe Pro Arg Lys Gly Val Arg Phe Val Thr Tyr Pro Asp
25 30 35
gaa tta ggt tta tcg aac gag ttg agt gaa aaa atc cag ctg tgg act 259
Glu Leu Gly Leu Ser Asn Glu Leu Ser Glu Lys Ile Gln Leu Trp Thr
40 45 50
cgc tac tgg tta gca aat ttt gtt gat gtc gaa gat gct ccc aaa ggg 307
Arg Tyr Trp Leu Ala Asn Phe Val Asp Val Glu Asp Ala Pro Lys Gly
55 60 65
cgt ccg cag tgg aaa gct gga tct gat gtt gag tcg tgg gta gcg caa 355
Arg Pro Gln Trp Lys Ala Gly Ser Asp Val Glu Ser Trp Val Ala Gln
70 75 80 85
ggt gac att att gaa agc gcg ctc cgt gca gaa ttg cct gat ttt gcg 403
Gly Asp Ile Ile Glu Ser Ala Leu Arg Ala Glu Leu Pro Asp Phe Ala
90 95 100
gtg ttc tca aaa tgg aga ttt tat gga ctc aat gtt cgt ttt gtg caa 451
Val Phe Ser Lys Trp Arg Phe Tyr Gly Leu Asn Val Arg Phe Val Gln
105 110 115
taaagtacgt ctttgaagtt gtaagtccaa 481
<210>414
<211>117
<212>PRT
<213>谷氨酸棒状杆菌
<400>414
Met Lys Lys Pro Thr Leu Tyr Leu Asn Leu Glu Tyr Gly Ile Arg Asn
1 5 10 15
Ser Leu Trp Ala Asp Lys Val Pro Phe Pro Arg Lys Gly Val Arg Phe
20 25 30
Val Thr Tyr Pro Asp Glu Leu Gly Leu Ser Asn Glu Leu Ser Glu Lys
35 40 45
Ile Gln Leu Trp Thr Arg Tyr Trp Leu Ala Asn Phe Val Asp Val Glu
50 55 60
Asp Ala Pro Lys Gly Arg Pro Gln Trp Lys Ala Gly Ser Asp Val Glu
65 70 75 80
Ser Trp Val Ala Gln Gly Asp Ile Ile Glu Ser Ala Leu Arg Ala Glu
85 90 95
Leu Pro Asp Phe Ala Val Phe Ser Lys Trp Arg Phe Tyr Gly Leu Asn
100 105 110
Val Arg Phe Val Gln
115
<210>415
<211>307
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(277)
<223>RXA04319
<400>415
tgtcggataa ccaggctcct gcggagttga agccgaagaa gtcttgagtt ttctgaaggc 60
tttctggagg ttttagagag aatcagcgcc cgggagctgt ttg gga ctc tcc tgg 115
Leu Gly Leu Ser Trp
1 5
gcg ctg att ttt gtt att tca ggg tgg ttt tcg ggt tta ttg tgg cct 163
Ala Leu Ile Phe Val Ile Ser Gly Trp Phe Ser Gly Leu Leu Trp Pro
10 15 20
ggg tat gcc ccg agt agg ccg atg tgc aag ggc gac tcc gca gaa ccg 211
Gly Tyr Ala Pro Ser Arg Pro Met Cys Lys Gly Asp Ser Ala Glu Pro
25 30 35
gac agg cgt gct aaa atg tcg aat ttt cag cac atc ttg gca cat gtg 259
Asp Arg Arg Ala Lys Met Ser Asn Phe Gln His Ile Leu Ala His Val
40 45 50
tct ggt ttc aag ctc tca taaccggaca cacgtgccaa aacctacgga 307
Ser Gly Phe Lys Leu Ser
55
<210>416
<211>59
<212>PRT
<213>谷氨酸棒状杆菌
<400>416
Leu Gly Leu Ser Trp Ala Leu Ile Phe Val Ile Ser Gly Trp Phe Ser
1 5 10 15
Gly Leu Leu Trp Pro Gly Tyr Ala Pro Ser Arg Pro Met Cys Lys Gly
20 25 30
Asp Ser Ala Glu Pro Asp Arg Arg Ala Lys Met Ser Asn Phe Gln His
35 40 45
Ile Leu Ala His Val Ser Gly Phe Lys Leu Ser
50 55
<210>417
<211>349
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(319)
<223>RXA04337
<400>417
ggcatctgtg actggtttcg aggtcttgaa accaaaccac gatgcccacg aatcatgaaa 60
atggatttgg tgggcaccgt ggtttgatcg cggcactcga atg ctg aat cag gcg 115
Met Leu Asn Gln Ala
1 5
ggg ctc ggg cca gac cag tgg gac aaa gaa tct gaa aat ccg aca tct 163
Gly Leu Gly Pro Asp Gln Trp Asp Lys Glu Ser Glu Asn Pro Thr Ser
10 15 20
ttg tca cat gtg cca ggt ttt gcg gag ttg aag agc cgc gga cac ccc 211
Leu Ser His Val Pro Gly Phe Ala Glu Leu Lys Ser Arg Gly His Pro
25 30 35
tcc cag ccc aag cga gca tgc aaa aag ctg cgc acc cca cct aaa caa 259
Ser Gln Pro Lys Arg Ala Cys Lys Lys Leu Arg Thr Pro Pro Lys Gln
40 45 50
acc gga aaa ccg gaa tcg cag gga tgc gca gct ctt aat tta caa aag 307
Thr Gly Lys Pro Glu Ser Gln Gly Cys Ala Ala Leu Asn Leu Gln Lys
55 60 65
act tat gcc ttg tagcggtcga ttgcctcagc gtggatcttt 349
Thr Tyr Ala Leu
70
<210>418
<211>73
<212>PRT
<213>谷氨酸棒状杆菌
<400>418
Met Leu Asn Gln Ala Gly Leu Gly Pro Asp Gln Trp Asp Lys Glu Ser
1 5 10 15
Glu Asn Pro Thr Ser Leu Ser His Val Pro Gly Phe Ala Glu Leu Lys
20 25 30
Ser Arg Gly His Pro Ser Gln Pro Lys Arg Ala Cys Lys Lys Leu Arg
35 40 45
Thr Pro Pro Lys Gln Thr Gly Lys Pro Glu Ser Gln Gly Cys Ala Ala
50 55 60
Leu Asn Leu Gln Lys Thr Tyr Ala Leu
65 70
<210>419
<211>313
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(283)
<223>RXA04352
<400>419
gcgctagaaa cttgtcccga ggggctgggt tggtgtgcac actgctgtgg ctttgtgtgt 60
gcacaaccta gtgggatagg gcgtgcacaa ggatatgaca ttg ggt gtg cac aac 115
Leu Gly Val His Asn
1 5
gtg ctg aca ctt tac aag ggg aaa cca gac agg cgt gac aaa aat cta 163
Val Leu Thr Leu Tyr Lys Gly Lys Pro Asp Arg Arg Asp Lys Asn Leu
10 15 20
gat ttc cgc cag gtt ttg gca cgc ctg tcc ggt tta ggg gat tgg aaa 211
Asp Phe Arg Gln Val Leu Ala Arg Leu Ser Gly Leu Gly Asp Trp Lys
25 30 35
ccg gac aca cgt gac aga act tcg agt ttt tcg cca atc ttg tca cgc 259
Pro Asp Thr Arg Asp Arg Thr Ser Ser Phe Ser Pro Ile Leu Ser Arg
40 45 50
ctg tct ggt ttg cct cga gaa ggg tgattcacgg ccaagacttc taaaagttcg 313
Leu Ser Gly Leu Pro Arg Glu Gly
55 60
<210>420
<211>61
<212>PRT
<213>谷氨酸棒状杆菌
<400>420
Leu Gly Val His Asn Val Leu Thr Leu Tyr Lys Gly Lys Pro Asp Arg
1 5 10 15
Arg Asp Lys Asn Leu Asp Phe Arg Gln Val Leu Ala Arg Leu Ser Gly
20 25 30
Leu Gly Asp Trp Lys Pro Asp Thr Arg Asp Arg Thr Ser Ser Phe Ser
35 40 45
Pro Ile Leu Ser Arg Leu Ser Gly Leu Pro Arg Glu Gly
50 55 60
<210>421
<211>322
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(292)
<223>RXA04354
<400>421
gtaaccgatg ttctgggagg gctgcggttg gaggtaaagc ctcatcgtgg caagtctagc 60
gataccttaa gcttttttaa accttttaca acagacgttc ttg ctt ctt tac aac 115
Leu Leu Leu Tyr Asn
1 5
aat cac ttt aag gag atc act atg ttt gat cag ttc cag cag ttc acc 163
Asn His Phe Lys Glu Ile Thr Met Phe Asp Gln Phe Gln Gln Phe Thr
10 15 20
gac att gca cag aac ttc cag ggc gca cag tgg ggt gtc cag att cag 211
Asp Ile Ala Gln Asn Phe Gln Gly Ala Gln Trp Gly Val Gln Ile Gln
25 30 35
gag cag ttc cag cag cac ttc cag aat tgg ttt aac acc gaa act gta 259
Glu Gln Phe Gln Gln His Phe Gln Asn Trp Phe Asn Thr Glu Thr Val
40 45 50
gag cca gcc aag agc act ttc tcc ctc agc tcc tagtcgctct ttaaggtcaa 312
Glu Pro Ala Lys Ser Thr Phe Ser Leu Ser Ser
55 60
tgtttcacgt 322
<210>422
<211>64
<212>PRT
<213>谷氨酸棒状杆菌
<400>422
Leu Leu Leu Tyr Asn Asn His Phe Lys Glu Ile Thr Met Phe Asp Gln
1 5 10 15
Phe Gln Gln Phe Thr Asp Ile Ala Gln Asn Phe Gln Gly Ala Gln Trp
20 25 30
Gly Val Gln Ile Gln Glu Gln Phe Gln Gln His Phe Gln Asn Trp Phe
35 40 45
Asn Thr Glu Thr Val Glu Pro Ala Lys Ser Thr Phe Ser Leu Ser Ser
50 55 60
<210>423
<211>436
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(406)
<223>RXA04370
<400>423
cgtcaccctt gtccttgcgg acaacctcga ggaatttatc cagggacggg gcaacagtgc 60
gctcatcctg agaaggatcg agaatgatca taagttcgta ttg acg cac gga cct 115
Leu Thr His Gly Pro
1 5
cat cac ctc cta tgg tct agt aat tgt ttt ttc ggc cat gcc gtc gtt 163
His His Leu Leu Trp Ser Ser Asn Cys Phe Phe Gly His Ala Val Val
10 15 20
ggc ata gca gga ggg tcg ttg cgt cag caa cct att caa ggt aca cca 211
Gly Ile Ala Gly Gly Ser Leu Arg Gln Gln Pro Ile Gln Gly Thr Pro
25 30 35
aac gcg cgg aaa tgt cga ttc ctg att tat gcg ttt tcg ttc aca gtt 259
Asn Ala Arg Lys Cys Arg Phe Leu Ile Tyr Ala Phe Ser Phe Thr Val
40 45 50
ccc gca tgg aag tta tgc tct gct tcc agc ggc ctc gct tca gcg gtg 307
Pro Ala Trp Lys Leu Cys Ser Ala Ser Ser Gly Leu Ala Ser Ala Val
55 60 65
gag atg ctt gcc aac gtc aaa gct gaa gcc gcg agc atg cct aca aag 355
Glu Met Leu Ala Asn Val Lys Ala Glu Ala Ala Ser Met Pro Thr Lys
70 75 80 85
gtg gtt gaa gtc tta gat ttt ttc atc tgt tac ctg cct ttc tac ctt 403
Val Val Glu Val Leu Asp Phe Phe Ile Cys Tyr Leu Pro Phe Tyr Leu
90 95 100
cct taacgcactt agttagagga aagttcccac 436
Pro
<210>424
<211>102
<212>PRT
<213>谷氨酸棒状杆菌
<400>424
Leu Thr His Gly Pro His His Leu Leu Trp Ser Ser Asn Cys Phe Phe
1 5 10 15
Gly His Ala Val Val Gly Ile Ala Gly Gly Ser Leu Arg Gln Gln Pro
20 25 30
Ile Gln Gly Thr Pro Asn Ala Arg Lys Cys Arg Phe Leu Ile Tyr Ala
35 40 45
Phe Ser Phe Thr Val Pro Ala Trp Lys Leu Cys Ser Ala Ser Ser Gly
50 55 60
Leu Ala Ser Ala Val Glu Met Leu Ala Asn Val Lys Ala Glu Ala Ala
65 70 75 80
Ser Met Pro Thr Lys Val Val Glu Val Leu Asp Phe Phe Ile Cys Tyr
85 90 95
Leu Pro Phe Tyr Leu Pro
100
<210>425
<211>1321
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1291)
<223>RXA04546
<400>425
aaactacgtt ggtagcaccg cggcgaagat aacttgcacc ggaattatgc gtggttacaa 60
gacgtgccaa atccatggga atcgtgctgt tcagtagctg ttg gta cac acc gac 115
Leu Val His Thr Asp
1 5
cgc gtc agc atc cat agg gcc aag aat ggt gta cga cgc acg cgg aaa 163
Arg Val Ser Ile His Arg Ala Lys Asn Gly Val Arg Arg Thr Arg Lys
10 15 20
cat att aaa acc atc tgt att aag cat ccc atc ggc aga tcc gta cag 211
His Ile Lys Thr Ile Cys Ile Lys His Pro Ile Gly Arg Ser Val Gln
25 30 35
ata agc cac tcg ccc acc gcg tta tct gat gcg gaa aac aat gct gaa 259
Ile Ser His Ser Pro Thr Ala Leu Ser Asp Ala Glu Asn Asn Ala Glu
40 45 50
ctc acc act gca cag att ctg cac att gac ccc gtc acc ggt gtg gcc 307
Leu Thr Thr Ala Gln Ile Leu His Ile Asp Pro Val Thr Gly Val Ala
55 60 65
atc cca cgt caa aac att ctg ctg ggt gag gat ttt cag act atg gcc 355
Ile Pro Arg Gln Asn Ile Leu Leu Gly Glu Asp Phe Gln Thr Met Ala
70 75 80 85
agc tct ttt agt caa atc cgc cat gga tgg ggt atc acc cag ctc atc 403
Ser Ser Phe Ser Gln Ile Arg His Gly Trp Gly Ile Thr Gln Leu Ile
90 95 100
gga caa gac ccc aac aga aac caa cca atc cgc tct gct gat acg tgg 451
Gly Gln Asp Pro Asn Arg Asn Gln Pro Ile Arg Ser Ala Asp Thr Trp
105 110 115
aca gtg act gga tcg act gga ctc acc ggc ttt aat act gct acg cat 499
Thr Val Thr Gly Ser Thr Gly Leu Thr Gly Phe Asn Thr Ala Thr His
120 125 130
aat aca gat gac aca gac gcc act gcc gca tat acc atg ccg gca agc 547
Asn Thr Asp Asp Thr Asp Ala Thr Ala Ala Tyr Thr Met Pro Ala Ser
135 140 145
gat cct gcc gta gga cta tgc gca ctg gaa tcc aac aaa gac gca ccg 595
Asp Pro Ala Val Gly Leu Cys Ala Leu Glu Ser Asn Lys Asp Ala Pro
150 155 160 165
gtg gat gaa ttt cgc gat ctc agc ctc agt gct tta cgt acc gct acg 643
Val Asp Glu Phe Arg Asp Leu Ser Leu Ser Ala Leu Arg Thr Ala Thr
170 175 180
gtc atg tca agc tct ggc tcc gcc gtg att act atg cat gat cct atg 691
Val Met Ser Ser Ser Gly Ser Ala Val Ile Thr Met His Asp Pro Met
185 190 195
gtc atg agc acc act ggc gca ttg gaa gca cgt gca tac gtt gac ggc 739
Val Met Ser Thr Thr Gly Ala Leu Glu Ala Arg Ala Tyr Val Asp Gly
200 205 210
gaa gtc atc aac cag cac gat ctt gac tca ctg cgt gac cag cta ggt 787
Glu Val Ile Asn Gln His Asp Leu Asp Ser Leu Arg Asp Gln Leu Gly
215 220 225
att act aca gac agc gca gat acc acc cct gcc cta cct gct gat ccc 835
Ile Thr Thr Asp Ser Ala Asp Thr Thr Pro Ala Leu Pro Ala Asp Pro
230 235 240 245
ttg gca gca ttg ggc ttg agc aca cca act aca tca gct ctt gtc cca 883
Leu Ala Ala Leu Gly Leu Ser Thr Pro Thr Thr Ser Ala Leu Val Pro
250 255 260
ggt ctt gcc gag ctg gac tgc ctc aac act gat caa gct cgc acc tgg 931
Gly Leu Ala Glu Leu Asp Cys Leu Asn Thr Asp Gln Ala Arg Thr Trp
265 270 275
cat gac cgt gac aac agc att ggc act ggt aaa cca gct att ctt gcc 979
His Asp Arg Asp Asn Ser Ile Gly Thr Gly Lys Pro Ala Ile Leu Ala
280 285 290
gtg att aac gcc gag ctc gct gat gat tac acc ctg cag atc ctc aaa 1027
Val Ile Asn Ala Glu Leu Ala Asp Asp Tyr Thr Leu Gln Ile Leu Lys
295 300 305
aat agc act gcg acc ccc acc gac tcc acc gac tcc agc gat act gct 1075
Asn Ser Thr Ala Thr Pro Thr Asp Ser Thr Asp Ser Ser Asp Thr Ala
310 315 320 325
gaa cgc ttc gta gcg cag cta cct gcg gaa aca gcc ttt gtg ctt att 1123
Glu Arg Phe Val Ala Gln Leu Pro Ala Glu Thr Ala Phe Val Leu Ile
330 335 340
gat ccg gac atc ggc gct gtg acc gat ctg ttc ttt atc aac agc ata 1171
Asp Pro Asp Ile Gly Ala Val Thr Asp Leu Phe Phe Ile Asn Ser Ile
345 350 355
aat cag gat ctc ccc gca ccg aca acc cag atc aac tca gta gcg gta 1219
Asn Gln Asp Leu Pro Ala Pro Thr Thr Gln Ile Asn Ser Val Ala Val
360 365 370
gat caa cgt gac ccc aac atc atc tac gcg acc ttt gcc aat gat gac 1267
Asp Gln Arg Asp Pro Asn Ile Ile Tyr Ala Thr Phe Ala Asn Asp Asp
375 380 385
cgc gtg tac cag ctc atg ctg ggt taaccgcgac ccgtttttat ctgtgctgat 1321
Arg Val Tyr Gln Leu Met Leu Gly
390 395
<210>426
<211>397
<212>PRT
<213>谷氨酸棒状杆菌
<400>426
Leu Val His Thr Asp Arg Val Ser Ile His Arg Ala Lys Asn Gly Val
1 5 10 15
Arg Arg Thr Arg Lys His Ile Lys Thr Ile Cys Ile Lys His Pro Ile
20 25 30
Gly Arg Ser Val Gln Ile Ser His Ser Pro Thr Ala Leu Ser Asp Ala
35 40 45
Glu Asn Asn Ala Glu Leu Thr Thr Ala Gln Ile Leu His Ile Asp Pro
50 55 60
Val Thr Gly Val Ala Ile Pro Arg Gln Asn Ile Leu Leu Gly Glu Asp
65 70 75 80
Phe Gln Thr Met Ala Ser Ser Phe Ser Gln Ile Arg His Gly Trp Gly
85 90 95
Ile Thr Gln Leu Ile Gly Gln Asp Pro Asn Arg Asn Gln Pro Ile Arg
100 105 110
Ser Ala Asp Thr Trp Thr Val Thr Gly Ser Thr Gly Leu Thr Gly Phe
115 120 125
Asn Thr Ala Thr His Asn Thr Asp Asp Thr Asp Ala Thr Ala Ala Tyr
130 135 140
Thr Met Pro Ala Ser Asp Pro Ala Val Gly Leu Cys Ala Leu Glu Ser
145 150 155 160
Asn Lys Asp Ala Pro Val ASp Glu Phe Arg Asp Leu Ser Leu Ser Ala
165 170 175
Leu Arg Thr Ala Thr Val Met Ser Ser Ser Gly Ser Ala Val Ile Thr
180 185 190
Met His Asp Pro Met Val Met Ser Thr Thr Gly Ala Leu Glu Ala Arg
195 200 205
Ala Tyr Val Asp Gly Glu Val Ile Asn Gln His Asp Leu Asp Ser Leu
210 215 220
Arg Asp Gln Leu Gly Ile Thr Thr Asp Ser Ala Asp Thr Thr Pro Ala
225 230 235 240
Leu Pro Ala Asp Pro Leu Ala Ala Leu Gly Leu Ser Thr Pro Thr Thr
245 250 255
Ser Ala Leu Val Pro Gly Leu Ala Glu Leu Asp Cys Leu Asn Thr Asp
260 265 270
Gln Ala Arg Thr Trp His Asp Arg Asp Asn Ser Ile Gly Thr Gly Lys
275 280 285
Pro Ala Ile Leu Ala Val Ile Asn Ala Glu Leu Ala Asp Asp Tyr Thr
290 295 300
Leu Gln Ile Leu Lys Asn Ser Thr Ala Thr Pro Thr Asp Ser Thr Asp
305 310 315 320
Ser Ser Asp Thr Ala Glu Arg Phe Val Ala Gln Leu Pro Ala Glu Thr
325 330 335
Ala Phe Val Leu Ile Asp Pro Asp Ile Gly Ala Val Thr Asp Leu Phe
340 345 350
Phe Ile Asn Ser Ile Asn Gln Asp Leu Pro Ala Pro Thr Thr Gln Ile
355 360 365
Asn Ser Val Ala Val Asp Gln Arg Asp Pro Asn Ile Ile Tyr Ala Thr
370 375 380
Phe Ala Asn Asp Asp Arg Val Tyr Gln Leu Met Leu Gly
385 390 395
<210>427
<211>829
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(799)
<223>RXA04549
<400>427
cgtttcgatc atttgtaaca ttcttattac aagactggaa ataaaggtga gtcaatcata 60
cagtcagatg tggtcatcgg caaaacagaa aggaatcccg atg tta cga aaa aca 115
Met Leu Arg Lys Thr
1 5
gtt acc ggt gga att gtt gct ctt att gcg act gcc act ctc atg aat 163
Val Thr Gly Gly Ile Val Ala Leu Ile Ala Thr Ala Thr Leu Met Asn
10 15 20
tct gtc tct tct gct gaa gag gta tcc gga gat gtc tca gca gta gat 211
Ser Val Ser Ser Ala Glu Glu Val Ser Gly Asp Val Ser Ala Val Asp
25 30 35
ctc gca ttt gca gaa ttc gaa cgt aca act gca gag gac gtg gcc aat 259
Leu Ala Phe Ala Glu Phe Glu Arg Thr Thr Ala Glu Asp Val Ala Asn
40 45 50
gga gtg cca gag gct ata gct aat gca gaa aaa att tcc aat ttc act 307
Gly Val Pro Glu Ala Ile Ala Asn Ala Glu Lys Ile Ser Asn Phe Thr
55 60 65
ccg agc gaa cta gag ggc ttc aag agt ctt tta gct agt gat gca gtg 355
Pro Ser Glu Leu Glu Gly Phe Lys Ser Leu Leu Ala Ser Asp Ala Val
70 75 80 85
ctg agg tcg gtc tct gga gaa gaa att ctc cca gat aat gtt gaa gtg 403
Leu Arg Ser Val Ser Gly Glu Glu Ile Leu Pro Asp Asn Val Glu Val
90 95 100
gtt cag acg gac aac gta ggc gac gaa gat cca aat gtc att tct cca 451
Val Gln Thr Asp Asn Val Gly Asp Glu Asp Pro Asn Val Ile Ser Pro
105 110 115
gca gcc tgg cag ggt tct gat tat att gag ggg tgt tta acg aat act 499
Ala Ala Trp Gln Gly Ser Asp Tyr Ile Glu Gly Cys Leu Thr Asn Thr
120 125 130
ctc tac gga att gaa gtc cta aag gtt tgt act ggt gga acc tac tac 547
Leu Tyr Gly Ile Glu Val Leu Lys Val Cys Thr Gly Gly Thr Tyr Tyr
135 140 145
tcc aat gta gga atc gct act tct gtc agc aat cct cgc agt tac gta 595
Ser Asn Val Gly Ile Ala Thr Ser Val Ser Asn Pro Arg Ser Tyr Val
150 155 160 165
aag tat aac agt gct cca gga ttg gca gtg acg aca agc aat cca cgt 643
Lys Tyr Asn Ser Ala Pro Gly Leu Ala Val Thr Thr Ser Asn Pro Arg
170 175 180
gga ggt ata gag ggt ggt ctt gcg gcc ttc tat ggt gat gtt aac ctt 691
Gly Gly Ile Glu Gly Gly Leu Ala Ala Phe Tyr Gly Asp Val Asn Leu
185 190 195
gtt gct ttt cca aat att cca tgg gtg gga cca att agt tct tct gca 739
Val Ala Phe Pro Asn Ile Pro Trp Val Gly Pro Ile Ser Ser Ser Ala
200 205 210
gga act cac cgc gtt gtc gct aga tca ttt ccg aat tca gtg gtg ctt 787
Gly Thr His Arg Val Val Ala Arg Ser Phe Pro Asn Ser Val Val Leu
215 220 225
aac gtc tac tac taatcgtttt ttagtttgaa acactgttgt 829
Asn Val Tyr Tyr
230
<210>428
<211>233
<212>PRT
<213>谷氨酸棒状杆菌
<400>428
Met Leu Arg Lys Thr Val Thr Gly Gly Ile Val Ala Leu Ile Ala Thr
1 5 10 15
Ala Thr Leu Met Asn Ser Val Ser Ser Ala Glu Glu Val Ser Gly Asp
20 25 30
Val Ser Ala Val Asp Leu Ala Phe Ala Glu Phe Glu Arg Thr Thr Ala
35 40 45
Glu Asp Val Ala Asn Gly Val Pro Glu Ala Ile Ala Asn Ala Glu Lys
50 55 60
Ile Ser Asn Phe Thr Pro Ser Glu Leu Glu Gly Phe Lys Ser Leu Leu
65 70 75 80
Ala Ser Asp Ala Val Leu Arg Ser Val Ser Gly Glu Glu Ile Leu Pro
85 90 95
Asp Asn Val Glu Val Val Gln Thr Asp Asn Val Gly Asp Glu Asp Pro
100 105 110
Asn Val Ile Ser Pro Ala Ala Trp Gln Gly Ser Asp Tyr Ile Glu Gly
115 120 125
Cys Leu Thr Asn Thr Leu Tyr Gly Ile Glu Val Leu Lys Val Cys Thr
130 135 140
Gly Gly Thr Tyr Tyr Ser Asn Val Gly Ile Ala Thr Ser Val Ser Asn
145 150 155 160
Pro Arg Ser Tyr Val Lys Tyr Asn Ser Ala Pro Gly Leu Ala Val Thr
165 170 175
Thr Ser Asn Pro Arg Gly Gly Ile Glu Gly Gly Leu Ala Ala Phe Tyr
180 185 190
Gly Asp Val Asn Leu Val Ala Phe Pro Asn Ile Pro Trp Val Gly Pro
195 200 205
Ile Ser Ser Ser Ala Gly Thr His Arg Val Val Ala Arg Ser Phe Pro
210 215 220
Asn Ser Val Val Leu Asn Val Tyr Tyr
225 230
<210>429
<211>484
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(454)
<223>RXA04642
<400>429
tatttagtgg aaatttcctg ttggtgttca tcgtgacggt cttgttgacg atcccagttg 60
ccgccatcag ctacaccttc atcgaagagc ccatcatgcg gtg gac ccg gcg cgc 115
Val Asp Pro Ala Arg
1 5
cat tca ggc tgg ggg tcg tta gga ttc acc att ttt ctg ggg gta ggt 163
His Ser Gly Trp Gly Ser Leu Gly Phe Thr Ile Phe Leu Gly Val Gly
10 15 20
ctg gaa aat gat gaa ttg gca cca cgt caa gac ggg gac ggg gca tgg 211
Leu Glu Asn Asp Glu Leu Ala Pro Arg Gln Asp Gly Asp Gly Ala Trp
25 30 35
caa gcc ggt aag gga act aat ttg cta ata ctc acc aca gga tcc gga 259
Gln Ala Gly Lys Gly Thr Asn Leu Leu Ile Leu Thr Thr Gly Ser Gly
40 45 50
ttt tca gcc gtt tgt ggt gga ttt ttg caa ctc gcc ggc ggt ttt atg 307
Phe Ser Ala Val Cys Gly Gly Phe Leu Gln Leu Ala Gly Gly Phe Met
55 60 65
caa aaa tcc gtt cga ttt gaa ggt ctg gcc aca gga ttg tgg tgg atc 355
Gln Lys Ser Val Arg Phe Glu Gly Leu Ala Thr Gly Let Trp Trp Ile
70 75 80 85
ctt gat caa caa gct gac agg aat cac agg agc ccc aat ggt cac aaa 403
Leu Asp Gln Gln Ala Asp Arg Asn His Arg Ser Pro Asn Gly His Lys
90 95 100
ttc tac cct ctc tac aat cgc ctc caa ggg cct aga atc gtc aga atg 451
Phe Tyr Pro Leu Tyr Asn Arg Leu Gln Gly Pro Arg Ile Val Arg Met
105 110 115
aac tgacacccat ttagcgcttt ggaggcctta 484
Asn
<210>430
<211>118
<212>PRT
<213>谷氨酸棒状杆菌
<400>430
Val Asp Pro Ala Arg His Ser Gly Trp Gly Ser Leu Gly Phe Thr Ile
1 5 10 15
Phe Leu Gly Val Gly Leu Glu Asn Asp Glu Leu Ala Pro Arg Gln Asp
20 25 30
Gly Asp Gly Ala Trp Gln Ala Gly Lys Gly Thr Asn Leu Leu Ile Leu
35 40 45
Thr Thr Gly Ser Gly Phe Ser Ala Val Cys Gly Gly Phe Leu Gln Leu
50 55 60
Ala Gly Gly Phe Met Gln Lys Ser Val Arg Phe Glu Gly Leu Ala Thr
65 70 75 80
Gly Leu Trp Trp Ile Leu Asp Gln Gln Ala Asp Arg Asn His Arg Ser
85 90 95
Pro Asn Gly His Lys Phe Tyr Pro Leu Tyr Asn Arg Leu Gln Gly Pro
100 105 110
Arg Ile Val Arg Met Asn
115
<210>431
<211>790
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(760)
<223>RXA06004
<400>431
agctgcctgc aagatggcgg cggagaagat tcccgctgga tccaaggtgt atgttttggg 60
ttcagaatcc ttccgcgagc tagctactga agctggtttt gtg gtg gtt gat tcg 115
Val Val Val Asp Ser
1 5
gct gat gat aaa cct gtg gct gtg ctt cac ggc cac aac cct gag acc 163
Ala Asp Asp Lys Pro Val Ala Val Leu His Gly His Asn Pro Glu Thr
10 15 20
ggt tgg gct cag ttg agc gag gct gcg ctg tca att aat gct ggc gcg 211
Gly Trp Ala Gln Let Ser Glu Ala Ala Leu Ser Ile Asn Ala Gly Ala
25 30 35
cag tat ttt gca tca aat ttg gat tcc acc ctt ccc atg gaa cgc ggt 259
Gln Tyr Phe Ala Ser Asn Leu Asp Ser Thr Leu Pro Met Glu Arg Gly
40 45 50
cgt cac att ggc aac ggt tcc atg gtg gct gcc gtg gtc aac gcg act 307
Arg His Ile Gly Asn Gly Ser Met Val Ala Ala Val Val Asn Ala Thr
55 60 65
ggc gta aag cct ctt tcc gca ggt aag cca ggc ccc gcg atg ttc tat 355
Gly Val Lys Pro Leu Ser Ala Gly Lys Pro Gly Pro Ala Met Phe Tyr
70 75 80 85
gcg ggg gct aaa act ctt aat tct tca aag cct ttg gct gtc ggc gat 403
Ala Gly Ala Lys Thr Leu Asn Ser Ser Lys Pro Leu Ala Val Gly Asp
90 95 100
cgt ctc gat act gat att gca ggc gga aac gct gca ggc atg gac aca 451
Arg Leu Asp Thr Asp Ile Ala Gly Gly Asn Ala Ala Gly Met Asp Thr
105 110 115
ttc cag gtc ctg acc ggc gtc agc ggc tac tac gat ttg gtg cgc gcc 499
Phe Gln Val Leu Thr Gly Val Ser Gly Tyr Tyr Asp Leu Val Arg Ala
120 125 130
att cca gag cag cgc ccc acc tat atc gcc acc tcg atg cag gat ctc 547
Ile Pro Glu Gln Arg Pro Thr Tyr Ile Ala Thr Ser Met Gln Asp Leu
135 140 145
tac agc gat ccg ggc gag ctc aag cca ggt gcc cag ggc ggt ttt tca 595
Tyr Ser Asp Pro Gly Glu Leu Lys Pro Gly Ala Gln Gly Gly Phe Ser
150 155 160 165
gcg ctt atc gac ggc gac acc ctg gtc att tcc ggc ggc gat gcc ggc 643
Ala Leu Ile Asp Gly Asp Thr Leu Val Ile Ser Gly Gly Asp Ala Gly
170 175 180
gca act ccg gtt gca gca ctc cgc act gcg ttg gat gtg gcc tgg gcg 691
Ala Thr Pro Val Ala Ala Leu Arg Thr Ala Leu Asp Val Ala Trp Ala
185 190 195
gcc aca gag cca gtc acc gag gta cgc gct gat tca gag gta gct gct 739
Ala Thr Glu Pro Val Thr Glu Val Arg Ala Asp Ser Glu Val Ala Ala
200 205 210
act gca ttg cag agc tgg tgg taaacggtga attcaccaaa gcctcatgat 790
Thr Ala Leu Gln Ser Trp Trp
215 220
<210>432
<211>220
<212>PRT
<213>谷氨酸棒状杆菌
<400>432
Val Val Val Asp Ser Ala Asp Asp Lys Pro Val Ala Val Leu His Gly
1 5 10 15
His Asn Pro Glu Thr Gly Trp Ala Gln Leu Ser Glu Ala Ala Leu Ser
20 25 30
Ile Asn Ala Gly Ala Gln Tyr Phe Ala Ser Asn Leu Asp Ser Thr Leu
35 40 45
Pro Met Glu Arg Gly Arg His Ile Gly Asn Gly Ser Met Val Ala Ala
50 55 60
Val Val Asn Ala Thr Gly Val Lys Pro Leu Ser Ala Gly Lys Pro Gly
65 70 75 80
Pro Ala Met Phe Tyr Ala Gly Ala Lys Thr Leu Asn Ser Ser Lys Pro
85 90 95
Leu Ala Val Gly Asp Arg Leu Asp Thr Asp Ile Ala Gly Gly Asn Ala
100 105 110
Ala Gly Met Asp Thr Phe Gln Val Leu Thr Gly Val Ser Gly Tyr Tyr
115 120 125
Asp Leu Val Arg Ala Ile Pro Glu Gln Arg Pro Thr Tyr Ile Ala Thr
130 135 140
Ser Met Gln Asp Leu Tyr Ser Asp Pro Gly Glu Leu Lys Pro Gly Ala
145 150 155 160
Gln Gly Gly Phe Ser Ala Leu Ile Asp Gly Asp Thr Leu Val Ile Ser
165 170 175
Gly Gly Asp Ala Gly Ala Thr Pro Val Ala Ala Leu Arg Thr Ala Leu
180 185 190
Asp Val Ala Trp Ala Ala Thr Glu Pro Val Thr Glu Val Arg Ala Asp
195 200 205
Ser Glu Val Ala Ala Thr Ala Leu Gln Ser Trp Trp
210 215 220
<210>433
<211>430
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(400)
<223>RXA06040
<400>433
cccgagtcta cgcaaaactg cactctgctg aacgctcatt tgaaaaaatg ggggatggtt 60
cggggtcttc atcaggggtg aaggtggtgc gcccttaggt ttg gag gga ctt cgt 115
Leu Glu Gly Leu Arg
1 5
aag att ccg gct tta ctt cgg gcg tca ctt cag gcc tgt atc gcc agt 163
Lys Ile Pro Ala Leu Leu Arg Ala Ser Leu Gln Ala Cys Ile Ala Ser
10 15 20
ttt ggc ctt ggc gcc cgc gat ttc gcc cca gac gac ctc aat tca agg 211
Phe Gly Leu Gly Ala Arg Asp Phe Ala Pro Asp Asp Leu Asn Ser Arg
25 30 35
ggg tcc aat cct aaa acc gga cta aaa cca gac tgt aaa gtg tca gca 259
Gly Ser Asn Pro Lys Thr Gly Leu Lys Pro Asp Cys Lys Val Ser Ala
40 45 50
cgt tgt gca cac tca ata tca tat cct tgc gca cgc ccg atc cca cta 307
Arg Cys Ala His Ser Ile Ser Tyr Pro Cys Ala Arg Pro Ile Pro Leu
55 60 65
ggt tgt gca cac aca aag cca cag cag tgt gca cac caa ccc tca ccc 355
Gly Cys Ala His Thr Lys Pro Gln Gln Cys Ala His Gln Pro Ser Pro
70 75 80 85
tcg gga caa gtt tct agc gca gta gat ccc ccg gat cta tcg act 400
Ser Gly Gln Val Ser Ser Ala Val Asp Pro Pro Asp Leu Ser Thr
90 95 100
taaatcgctt ctcagcctca tcacgtttgc 430
<210>434
<211>100
<212>PRT
<213>谷氨酸棒状杆菌
<400>434
Leu Glu Gly Leu Arg Lys Ile Pro Ala Leu Leu Arg Ala Ser Leu Gln
1 5 10 15
Ala Cys Ile Ala Ser Phe Gly Leu Gly Ala Arg Asp Phe Ala Pro Asp
20 25 30
Asp Leu Asn Ser Arg Gly Ser Asn Pro Lys Thr Gly Leu Lys Pro Asp
35 40 45
Cys Lys Val Ser Ala Arg Cys Ala His Ser Ile Ser Tyr Pro Cys Ala
50 55 60
Arg Pro Ile Pro Leu Gly Cys Ala His Thr Lys Pro Gln Gln Cys Ala
65 70 75 80
His Gln Pro Ser Pro Ser Gly Gln Val Ser Ser Ala Val Asp Pro Pro
85 90 95
Asp Leu Ser Thr
100
Claims (8)
1.分离的核酸分子,其编码具有在每种情况下表1/第2列中所示的氨基酸序列的多肽,其中该核酸分子在表1/第4列所示氨基酸位置处编码与表1/第5列相同行中所示的特定氨基酸不同的蛋白原性氨基酸。
2.如权利要求1的分离的核酸分子,其中该核酸分子在表1/第4列中所示的氨基酸位置处编码表1/第6列相同行中所示的氨基酸。
3.载体,其含有至少一种如权利要求1的核酸序列。
4.宿主细胞,其被至少一种如权利要求3的载体转染。
5.如权利要求4的宿主细胞,其中所述核酸分子的表达调节所述细胞中精细化学品的生产。
6.一种生产精细化学品的方法,其包括培养用至少一种权利要求3的载体转染的细胞从而生产该精细化学品。
7.如权利要求6的方法,其中精细化学品是氨基酸。
8.如权利要求7的方法,其中所述氨基酸是赖氨酸。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10154177.5 | 2001-11-05 | ||
DE10154177A DE10154177A1 (de) | 2001-11-05 | 2001-11-05 | Gene die für neue Proteine codieren |
Publications (1)
Publication Number | Publication Date |
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CN1582300A true CN1582300A (zh) | 2005-02-16 |
Family
ID=7704612
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNA028241207A Pending CN1582300A (zh) | 2001-11-05 | 2002-10-31 | 编码新蛋白的基因 |
Country Status (9)
Country | Link |
---|---|
US (1) | US20050003494A1 (zh) |
EP (1) | EP1446421A2 (zh) |
KR (1) | KR100868692B1 (zh) |
CN (1) | CN1582300A (zh) |
AU (1) | AU2002365499A1 (zh) |
BR (1) | BR0213779A (zh) |
DE (1) | DE10154177A1 (zh) |
WO (1) | WO2003046123A2 (zh) |
ZA (1) | ZA200404418B (zh) |
Cited By (9)
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CN109694841A (zh) * | 2019-02-02 | 2019-04-30 | 江南大学 | 一种谷氨酸棒状杆菌重组菌、制备方法和应用 |
CN109929887A (zh) * | 2017-12-15 | 2019-06-25 | 宁夏伊品生物科技股份有限公司 | 改进的发酵生产l-赖氨酸的方法 |
CN111979164A (zh) * | 2020-08-07 | 2020-11-24 | 宁夏伊品生物科技股份有限公司 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
WO2021143727A1 (zh) * | 2020-01-15 | 2021-07-22 | 中国科学院天津工业生物技术研究所 | 生产赖氨酸的微生物以及赖氨酸的生产方法 |
CN113430182A (zh) * | 2021-08-09 | 2021-09-24 | 云南师范大学 | 一种来源于亚洲象肠道毛螺菌科的细菌漆酶及其基因 |
WO2022027924A1 (zh) * | 2020-08-07 | 2022-02-10 | 宁夏伊品生物科技股份有限公司 | 一种产l-氨基酸的重组菌株及其构建方法与应用 |
CN115052883A (zh) * | 2021-01-28 | 2022-09-13 | Cj第一制糖株式会社 | 新蛋白变体及使用其生产l-缬氨酸的方法 |
CN115322990A (zh) * | 2021-05-10 | 2022-11-11 | 中国科学院天津工业生物技术研究所 | 具有启动子活性的多核苷酸及其在生产目标化合物中的用途 |
CN115925829A (zh) * | 2022-07-22 | 2023-04-07 | 东北农业大学 | rHtaA-c蛋白在制备用于预防化脓隐秘杆菌的疫苗中的应用 |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10359661A1 (de) | 2003-12-18 | 2005-07-28 | Basf Ag | Genvarianten die für Proteine aus dem Stoffwechselweg von Feinchemikalien codieren |
AU2005320486B2 (en) * | 2004-12-28 | 2009-08-13 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
US7794989B2 (en) | 2004-12-28 | 2010-09-14 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
JP2019519243A (ja) * | 2016-07-05 | 2019-07-11 | ザイマージェン インコーポレイテッド | Rnaデグラドソームタンパク質複合体の遺伝的攪乱 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5925427A (en) * | 1995-02-06 | 1999-07-20 | Minnesota Mining And Manufacturing Company | Support core ribbon for cold-shrink tube |
JP4623825B2 (ja) * | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
-
2001
- 2001-11-05 DE DE10154177A patent/DE10154177A1/de not_active Withdrawn
-
2002
- 2002-10-31 EP EP02803765A patent/EP1446421A2/de not_active Withdrawn
- 2002-10-31 AU AU2002365499A patent/AU2002365499A1/en not_active Abandoned
- 2002-10-31 US US10/494,672 patent/US20050003494A1/en not_active Abandoned
- 2002-10-31 WO PCT/EP2002/012134 patent/WO2003046123A2/de not_active Application Discontinuation
- 2002-10-31 KR KR1020047006758A patent/KR100868692B1/ko not_active IP Right Cessation
- 2002-10-31 CN CNA028241207A patent/CN1582300A/zh active Pending
- 2002-10-31 BR BR0213779-8A patent/BR0213779A/pt not_active IP Right Cessation
-
2004
- 2004-06-04 ZA ZA200404418A patent/ZA200404418B/xx unknown
Cited By (11)
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CN109929887A (zh) * | 2017-12-15 | 2019-06-25 | 宁夏伊品生物科技股份有限公司 | 改进的发酵生产l-赖氨酸的方法 |
CN109694841A (zh) * | 2019-02-02 | 2019-04-30 | 江南大学 | 一种谷氨酸棒状杆菌重组菌、制备方法和应用 |
WO2021143727A1 (zh) * | 2020-01-15 | 2021-07-22 | 中国科学院天津工业生物技术研究所 | 生产赖氨酸的微生物以及赖氨酸的生产方法 |
CN111979164A (zh) * | 2020-08-07 | 2020-11-24 | 宁夏伊品生物科技股份有限公司 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
CN111979164B (zh) * | 2020-08-07 | 2021-11-12 | 宁夏伊品生物科技股份有限公司 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
WO2022027924A1 (zh) * | 2020-08-07 | 2022-02-10 | 宁夏伊品生物科技股份有限公司 | 一种产l-氨基酸的重组菌株及其构建方法与应用 |
CN115052883A (zh) * | 2021-01-28 | 2022-09-13 | Cj第一制糖株式会社 | 新蛋白变体及使用其生产l-缬氨酸的方法 |
CN115322990A (zh) * | 2021-05-10 | 2022-11-11 | 中国科学院天津工业生物技术研究所 | 具有启动子活性的多核苷酸及其在生产目标化合物中的用途 |
CN113430182A (zh) * | 2021-08-09 | 2021-09-24 | 云南师范大学 | 一种来源于亚洲象肠道毛螺菌科的细菌漆酶及其基因 |
CN115925829A (zh) * | 2022-07-22 | 2023-04-07 | 东北农业大学 | rHtaA-c蛋白在制备用于预防化脓隐秘杆菌的疫苗中的应用 |
CN115925829B (zh) * | 2022-07-22 | 2024-04-23 | 东北农业大学 | rHtaA-c蛋白在制备用于预防化脓隐秘杆菌的疫苗中的应用 |
Also Published As
Publication number | Publication date |
---|---|
EP1446421A2 (de) | 2004-08-18 |
KR100868692B1 (ko) | 2008-11-13 |
AU2002365499A1 (en) | 2003-06-10 |
WO2003046123A3 (de) | 2004-02-12 |
DE10154177A1 (de) | 2003-05-08 |
US20050003494A1 (en) | 2005-01-06 |
ZA200404418B (en) | 2006-07-26 |
BR0213779A (pt) | 2004-10-13 |
KR20040053275A (ko) | 2004-06-23 |
WO2003046123A2 (de) | 2003-06-05 |
AU2002365499A8 (en) | 2003-06-10 |
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