CN1582299A

CN1582299A - 编码与遗传稳定性、基因表达和折叠相关的蛋白质的基因

Info

Publication number: CN1582299A
Application number: CNA028233409A
Authority: CN
Inventors: O·策尔德尔; M·蓬佩尤斯; H·施罗德; B·克勒格尔; C·克洛普罗格; G·哈伯豪尔
Original assignee: BASF SE
Current assignee: Paik Kwang Industrial Co Ltd
Priority date: 2001-11-05
Filing date: 2002-10-31
Publication date: 2005-02-16
Anticipated expiration: 2022-10-31
Also published as: EP1693380A2; DE10154180A1; US20070054381A1; KR100861746B1; US20070072273A1; ATE429443T1; US7323559B2; US20050009152A1; EP1693380A3; US7339048B2; US7355028B2; WO2003040180A3; BR0213771A; KR20050042246A; WO2003040180A2; EP1693380B1; CN1323087C; EP1444258A2; DE50213492D1; ZA200404424B

Abstract

本发明涉及新核酸分子，涉及其构建遗传改良的微生物的用途，还涉及在所述遗传改良微生物的帮助下制备精细化学品，尤其是氨基酸的方法。

Description

编码与遗传稳定性、基因表达和折叠相关的蛋白质的基因

发明背景

细胞中天然进行的代谢过程所产生的特定产物和副产物可以用于工业的许多分支中，包括食品工业、动物饲料工业、化妆品工业及制药工业。这些分子合起来称为“精细化学品”，其包括有机酸、蛋白原性(protainogenic)和非蛋白原性氨基酸、核苷酸和核苷、脂类和脂肪酸、二醇、碳水化合物、芳香化合物、维生素和辅因子、以及酶。它们的最好的产生途径是大规模培养细菌，针对不同的特定情况，这些细菌被开发出来以产生并分泌大量的期望分子。一种对此目的尤其适用的生物体是谷氨酸棒状杆菌(Corynebacterium glutamicum)，其为革兰氏阳性的非致病细菌。通过菌株选择，已开发了许多突变菌株，它们产生各种期望化合物。但是，对具有提高的特定分子产量的菌株的筛选是一种费时且困难的方法。

发明简述

本发明提供了新的可用于谷氨酸棒状杆菌或相关菌种的鉴定或分类的核酸分子。谷氨酸棒状杆菌为革兰氏阳性需氧细菌，其通常广泛地用在工业上用于大规模产生多种精细化学品，也用于降解碳氢化合物(如在原油泄漏物中)，以及用于氧化萜类化合物。本发明的核酸分子因此可用于鉴定能如通过发酵法用来产生精细化学品的微生物。虽然谷氨酸棒状杆菌自身为非致病的，但它与作为人类的主要病原体的其它谷氨酸棒状杆菌菌种，如白喉棒状杆菌(Corynebacterium diphtheriae)(白喉的致病因子)有亲缘关系。所以能够鉴定棒状杆菌的存在也具有显著的临床重要性，例如诊断应用。而且，所述核酸分子可作为谷氨酸棒状杆菌或相关生物基因组作图的参考点。

这些新核酸分子编码的蛋白质文中称为遗传稳定性、基因表达或蛋白质分泌/蛋白质折叠相关蛋白(SES蛋白)。这些SES蛋白可以例如发挥与谷氨酸棒状杆菌DNA修复或重组、遗传物质转座、基因表达(即参与转录或翻译)、蛋白质折叠或蛋白质分泌相关的功能。由于可得到用于谷氨酸棒状杆菌中的克隆载体(如在Sinskey等人，美国专利号4,649,119中所公开的)和用于遗传操作谷氨酸棒状杆菌和相关短杆菌种(如乳发酵短杆菌)的技术(Yoshihama等人，细菌学杂志(J.Bacteriol.)162：591-597(1985)；Katsumata等人，细菌学杂志(J.Bacteriol.)159：306-311(1984)；和Santamaria等，J.Gen.Microbiol.130：2237-2246(1984))，本发明的核酸分子可用于该生物体的遗传操作，使其成为一种或多种精细化学品的更有效的生产者。通过操作本发明的基因可以直接或间接地导致精细化学品产量和/或生产效率的提高。

存在许多机制，通过这些机制本发明的SES蛋白的修饰能够直接影响含有该修饰蛋白的谷氨酸棒状杆菌菌株生产精细化学品的产率、产量和/或生产效率。例如，对直接参与转录或翻译的蛋白(例如聚合酶或核糖体)进行调节以增加它们的数量或活性应能总体上增加细胞的转录或翻译(或这些过程的速率)。该增加的细胞基因表达将包括那些参与精细化学品生物合成的蛋白，从而使一种或多种目标化合物的产率、产量和/或生产效率能得以增加。通过修饰谷氨酸棒状杆菌的转录/翻译蛋白机器从而改变这些蛋白的调节作用也可以增加参与精细化学品生产的基因的表达。对许多参与肽折叠的蛋白的活性的调节可以增加细胞中正确折叠的分子的总体生产，从而增加目标蛋白(例如生物合成精细化学品的蛋白)正确发挥功能的可能性。此外，可通过突变参与谷氨酸棒状杆菌分泌的蛋白，从而增加它们的数量或活性，以便增加精细化学品(例如酶)从细胞向发酵培养物中的分泌，而从发酵培养物中可容易地得到所述精细化学品。

本发明SES分子的遗传修饰也可以间接调节一种或多种精细化学品的生产。例如，可能通过增加本发明的DNA修复或DNA重组蛋白的数量或活性来提高细胞检测并修复DNA损伤的能力。这将有效增加细胞将突变基因保留在基因组中的能力，从而增加通过遗传方法导入谷氨酸棒状杆菌中的转基因(其编码例如可以增加精细化学品的生物合成的蛋白)在微生物的培养中不发生丢失的可能性。相反，可以通过减少一种或多种DNA修复或DNA重组蛋白的数量或活性来增加生物体的遗传不稳定性。此操作将增加所述生物体被诱变而发生修饰且不纠正导入的突变的能力。对于参与谷氨酸棒状杆菌中遗传元件转座或重排的蛋白质(例如转座子)，这也是一样的。通过诱变这些蛋白以增加或降低蛋白数量或活性使得可能同时增加或降低微生物的遗传稳定性。这关键性地影响向谷氨酸棒状杆菌中导入另一个突变和保留所导入的突变的可能性。转座子同样提供了一种可以诱变谷氨酸棒状杆菌的合适的机制，通过转座子诱变可以容易地进行目标基因(例如生物合成精细化学品的基因)的复制和不想要的基因(例如参与目标精细化学品的降解的基因)的破坏。

可能可以通过调节一种或多种参与反应特定环境条件而调节转录或翻译的蛋白(例如σ因子)，以防止细胞在不利环境条件(如大规模发酵培养中存在的不利条件)下减慢或停止蛋白的合成。这将增加基因表达，而这反过来可增加在所述条件下目标精细化学品的生物合成。与分泌系统相关的蛋白的突变可导致分泌速率受到调节。许多这些分泌蛋白(例如细胞表面蛋白酶或细胞表面受体)具有对细胞存活重要的功能。当改变分泌途径从而这些蛋白更容易运输到它们的胞外地点时可能增加细胞总的存活力，从而导致更多数量的谷氨酸棒状杆菌细胞能够在大规模培养中生产精细化学品。还知道分泌器(例如sec系统)也参与整合膜蛋白(例如孔、通道或转运蛋白)向膜的插入。从而，对参与谷氨酸棒状杆菌中蛋白分泌的蛋白的活性的调节可以影响细胞分泌废产物或输入必需代谢物的能力。如果这些分泌蛋白的活性增加，那么细胞产生精细化学品的能力同样会增加。如果所述分泌蛋白的活性降低，则可能没有足够的营养来支持目标化合物的过量生产或者废产物可能干扰该生物合成。

本发明提供了新的核酸分子，它们编码本文称作SES蛋白的蛋白，这些蛋白可参与谷氨酸棒状杆菌中例如DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌。编码SES蛋白的核酸分子在本文中称作SES核酸分子。在一个优选的实施方案中，SES蛋白参与提高或降低谷氨酸棒状杆菌中的遗传稳定性，参与基因表达(例如参与转录或翻译)或参与该生物中蛋白质折叠或参与谷氨酸棒状杆菌的蛋白质分泌。这些蛋白质的实例为表1中所列基因编码的蛋白。

因此，本发明的一个方面涉及含有编码SES蛋白或其生物学活性部分的核苷酸序列的分离的核酸分子(例如cDNA)和适合作为检测或扩增SES编码核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在尤其优选的实施方案中，该分离的核酸分子含有集合A所列出的任一核酸序列或这些核酸序列之任一的编码区或互补序列。在其他优选的实施方案中，该分离的核酸分子编码集合B中列出的任一氨基酸序列。本发明优选的SES蛋白也优选具有至少一种本文所描述的SES活性。

以下将序列表中的核酸序列以及下表1中描述的在相关位置处的序列修饰一起定义为集合A。

以下将序列表中的多肽序列以及下表1中描述的在相关位置处的序列修饰一起定义为集合B。

在另一个实施方案中，分离的核酸分子长度至少15个核苷酸并且在严紧条件下与含有集合A的核苷酸序列的核酸分子杂交。分离的核酸分子优选与天然发生的核酸分子对应。分离的核酸更优选编码天然发生的谷氨酸棒状杆菌SES蛋白或其具生物活性的部分。

本发明的另一个方面涉及载体(例如重组表达载体)，其含有本发明的核酸分子，还涉及已导入所述载体的宿主细胞。在一个实施方案中，通过使用在合适的培养基中培养的宿主细胞制备SES蛋白。可以从培养基或宿主细胞中分离该SES蛋白。

本发明的另一个方面涉及其中导入了SES基因或其SES基因被修饰的遗传修饰的微生物。在一个实施方案中，通过导入至少一种编码突变SES序列的本发明核酸分子作为转基因，修饰了所述微生物的基因组。在另一个实施方案中，通过与修饰的SES基因的同源重组，修饰(例如破坏功能)了所述微生物基因组中的内源性SES基因。在一个优选的实施方案中，微生物属于棒状杆菌或短杆菌(Brevibacterium)属，尤其优选谷氨酸棒状杆菌。在一个优选的实施方案中，该微生物也被用于制备目标化合物，如氨基酸、尤其优选赖氨酸。

本发明的另一方面涉及分离的SES蛋白或其一部分，例如生物学活性部分。在一个优选的实施方案中，分离的SES蛋白或其部分可参与谷氨酸棒状杆菌中DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌。在另一个优选的实施方案中，分离的SES蛋白或其部分与集合B的氨基酸序列充分同源从而保持例如参与谷氨酸棒状杆菌中DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌的能力。

另一个优选的实施方案是具有一种以上的集合A中描述的核酸分子的宿主细胞。可用各种技术人员已知的方法制备这些宿主细胞。例如，可通过携带几种本发明核酸分子的载体转染这些细胞。然而，也可以使用载体每一种情况下向宿主细胞中导入一种本发明的核酸分子并从而同时或相继使用多个载体。从而可能构建携带许多乃至高达数百的本发明核酸序列的宿主细胞。这种积累常能够对宿主细胞的精细化学品的生产力产生超加性效应。

此外，本发明提供分离的SES蛋白制品。在优选的实施方案中，该SES蛋白含有集合B的氨基酸序列。在另一个优选的实施方案中，本发明涉及分离的完整长度的蛋白，其和集合B的完整氨基酸序列(其由集合A中的可读框编码)基本同源。

可以将SES多肽或其生物学活性部分与非SES多肽功能性地连接以产生融合蛋白。在优选的实施方案中，该融合蛋白和单独的SES蛋白具有不同的活性，在其它优选的实施方案中，所述融合蛋白参与谷氨酸棒状杆菌中DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌。在尤其优选的实施方案中，所述融合蛋白整合在宿主细胞中调节细胞对目标化合物的生产。

本发明的另一个方面涉及制备精细化学品的制备方法。该方法涉及含有可导致本发明SES核酸分子表达的载体的细胞的培养，从而生产精细化学品。在优选的实施方案中，该方法还包括得到含有此载体的细胞的步骤，其中所述细胞被可以导致SES核酸表达的载体转染。在另一个优选的实施方案中，所述方法还包括从培养物中得到精细化学品的步骤。在一个尤其优选的实施方案中，该细胞属于棒状杆菌属或短杆菌属。

本发明的另一个方面涉及调节微生物生产分子的方法。这些方法包括将细胞和调节SES蛋白活性或SES核酸表达的物质接触使得和没有所述物质存在时相比细胞的相关活性被改变。在一个优选的实施方案中，针对一个或多个参与谷氨酸棒状杆菌中遗传稳定性、基因表达、蛋白质折叠或蛋白质分泌的过程对细胞进行调节，以提高该微生物生产目标精细化学品的产率、产量或生产效率。调节SES蛋白活性的物质可以是刺激SES蛋白活性或SES核酸表达的物质。刺激SES蛋白活性或SES核酸表达的物质的例子包括小分子、活性SES蛋白和已经被导入细胞中的编码SES蛋白的核酸分子。抑制SES活性或SES表达的物质的例子包括小分子和反义SES核酸分子。

本发明的另一个方面涉及调节细胞中目的化合物的产率的方法，包括向细胞中导入SES野生型基因或HA突变基因，该基因或者保留在分开的质粒上或者整合到宿主细胞的基因组中。向基因组的整合可随机发生或者通过同源重组发生，使得天然基因被整合的拷贝所代替，导致细胞中目标化合物的生产被调节。在另一个优选的实施方案中，所述产率增加了。在另一个优选的实施方案中，化学品是精细化学品，其在一个特别优选的实施方案中是氨基酸。在一个特别优选的实施方案中，该氨基酸是L-赖氨酸。

发明详述

本发明提供了SES核酸和SES蛋白分子，它们参与谷氨酸棒状杆菌中DNA的修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌。本发明的分子可用于调节微生物如谷氨酸棒状杆菌的精细化学品的生产，这种调节可以为直接调节(例如，如果参与精细化学品(例如酶)的分泌的蛋白被过表达或者其活性被优化，则可以直接影响修饰的谷氨酸棒状杆菌细胞的精细化学品生产的产率、产量和/或效率)或者为间接调节但仍然导致目标化合物的产率、产量和/或效率增加(例如，如果调节谷氨酸棒状杆菌DNA修复蛋白的活性或拷贝数从而改变该微生物保持导入的突变的能力，则反过来可以影响所述菌株的一种或多种精细化学品的生产)。下面进一步阐述本发明的各方面。

I.精细化学品

术语“精细化学品”为本领域所认知，其包括已用于多种工业的由生物体产生的分子，其中所述工业例如但不限于制药工业、农业工业和化妆品工业。此类化合物包括有机酸如酒石酸、衣康酸和二氨基庚二酸、蛋白原的和非蛋白原的氨基酸、嘌呤和嘧啶碱基、核苷和核苷酸(如在Rehm等人编辑的生物技术(Biotechnology)第6卷中Kuninaka，A.(1996)核苷酸和相关化合物(Nucleotides and related compounds)，561-612页，VCH：Weinheim及其中所含参考文献中所述)、脂类、饱和和不饱和脂肪酸(如花生四烯酸)、二醇(如丙二醇和丁二醇)、碳水化合物(如透明质酸和海藻糖)、芳香化合物(如芳香胺、香草醛和靛蓝)、维生素和辅因子(参见如Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)，A27卷，“维生素”(“Vitamins”)，443-613页(1996)VCH：Weinheim及其中的参考文献；以及Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition，Lipids，Health，and Disease”Proceedings ofthe UNESCO/Confederation of Scientific and Technological Associationsin Malaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press，(1995))、酶以及在Gutcho(1983)《通过发酵的化学品》(Chemicals by Fermentation)，Noyes DataCorporation，ISBN：0818805086和其中的参考文献中所述的所有其它化学品。这些精细化学品中的某些化学品的代谢和用途进一步阐述如下。

A.氨基酸代谢和用途

氨基酸包含所有蛋白质的基本结构单元，由此为所有生物体中正常细胞功能所必需。术语“氨基酸”为本领域所认知。蛋白原性氨基酸有20种，为蛋白质的结构单元，在蛋白质中它们通过肽键连接，非蛋白原性氨基酸(已知成百种非蛋白原的氨基酸)在蛋白质中通常不存在(参见Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry))，A2卷，57-97页，VCH：Weinheim(1985))。氨基酸可为光学D-或L-构型，尽管在天然存在的蛋白质中发现的唯一类型通常为L-氨基酸。已充分说明了20种蛋白原性氨基酸中每一种在原核和真核细胞中的生物合成和降解途径(参见例如，Stryer，L.生物化学(Biochemistry)，第三版，578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)，这样称呼是因为由于它们生物合成的复杂性，通常必须从食物中摄取它们，它们通过简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物能够合成这些氨基酸中的一部分氨基酸，但必需氨基酸必须从食物中摄取，以进行正常的蛋白质合成。

除了它们在蛋白质生物合成中的功能外，这些氨基酸本身还是令人感兴趣的化学品，并且许多已发现在人类食品、动物饲料、化学品、化妆品、农业和药学工业中具有多种应用。赖氨酸不仅对人的营养而且对单胃家畜如禽和猪都是一种重要的氨基酸。谷氨酸盐是最常用的调味添加剂(谷氨酸单钠盐，MSG)，并且还用于食品工业，天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸均用于药学工业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸用于药学工业和化妆品工业。苏氨酸、色氨酸和D/L-甲硫氨酸是广泛使用的动物饲料添加剂。(Rehm等人(编辑)，生物技术(Biotechnology)，第6卷，第14a章中的Leuchtenberger，W.(1996)Amino aids-technicalproduction and use，466-502页，VCH：Weinheim)。还发现这些氨基酸适用作前体，用于合成合成的氨基酸和蛋白质，如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟色氨以及在Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)，A2卷，57-97页，VCH：Weinheim，1985中所述的其它物质。

在可产生这些天然氨基酸的生物体如细菌中已充分描述了它们的生物合成(细菌氨基酸生物合成及其调节的综述，见Umbarger，H.E.(1978)Ann.Rev.Biochem.47：533-606)。谷氨酸通过还原胺化α-酮戊二酸(柠檬酸循环中的中间体)而合成。谷氨酰胺、脯氨酸和精氨酸接下来由谷氨酸产生。丝氨酸的生物合成为三步，由3-磷酸甘油酸(糖酵解中间体)开始，在氧化、氨基转移和水解步骤后产生该氨基酸。半胱氨酸和甘氨酸两者均产生自丝氨酸；具体地，前者通过高半胱氨酸与丝氨酸缩合，后者在丝氨酸转羟甲基酶催化的反应中通过侧链β-碳原子转移至四氢叶酸上合成。苯丙氨酸和酪氨酸在9步的生物合成途径中合成自糖酵解和磷酸戊糖途径的前体赤藓糖4-磷酸和磷酸烯醇丙酮酸，它们的不同仅在预苯酸合成后的最后两步。色氨酸也从这两种起始分子产生，但其合成为11步的途径。酪氨酸也可在苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸均为来自丙酮酸盐(糖酵解的终产物)的生物合成产物。天冬氨酸形成自草酰乙酸(柠檬酸循环的中间体)。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸均通过转化天冬氨酸而形成。异亮氨酸从苏氨酸形成。组氨酸在一个复杂的9步途径中从一种活化糖——5-磷酸核糖-1-焦磷酸形成。

超过蛋白质生物合成所需的量的氨基酸不能被贮藏，并被降解以提供用于细胞主要代谢途径的中间体(综述参见Stryer，L.生物化学(Biochemistry)第三版，21章，“氨基酸降解和尿素循环”495-516页(1988))。虽然细胞可将不想要的氨基酸转化为有用的代谢中间体，但从所需用于合成氨基酸的能量、前体分子和酶来说，氨基酸的产生是昂贵的。因此对于氨基酸的生物合成受到反馈抑制调节并不让人吃惊，其中特定氨基酸的存在起到减缓或完全终止其自身产生的作用(关于氨基酸生物合成途径中的反馈机制的综述参见Stryer，L.生物化学(Biochemistry)第三版，24章：”氨基酸和血红素的生物合成”575-600页(1988))。因此，特定氨基酸的输出受到细胞中存在的该氨基酸的量的限制。

B.维生素、辅因子和营养成分的代谢和用途

维生素、辅因子和营养成分包含高等动物不能合成且必须摄取的另一类分子，虽然它们易于被其它生物体如细菌合成。这些分子自身为生物活性物质或为在多种代谢途径中作为电子载体或中间体的生物活性物质的前体。除了它们的营养价值外，这些化合物作为着色剂、抗氧化剂和催化剂或其它操作辅助剂也具有重要的工业价值(这些化合物的结构、活性和工业应用的概述参见例如，Ullmann工业化学百科全书(Ullmann’sEncyclopedia of Industrial Chemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996)。术语”维生素”为本领域认知，包括生物体行使正常功能所需的营养素，且该生物体自身不能合成它们。维生素组可以包括辅因子和营养性化合物。术语“辅因子”包括进行正常酶促活性所需的非蛋白质性化合物。此类化合物可为有机的或无机的，本发明的辅因子优选有机的。术语“营养成分”包括促进植物和动物尤其是人的健康的食物添加剂。此类分子的实例为维生素、抗氧化剂和某些脂类(如多不饱和脂肪酸)。

已大量描述了这些分子在可产生它们的生物体如细菌中的生物合成(Ullmann工业化学百科全书(Ullmann’s Encyclopedia of IndustrialChemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wiley& Sons；Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition，Lipids，Health，and Disease”Proceedings of theUNESCO/Confederation of Scientific and Technological Associations inMalaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press：Champaign，IL X，374S)。

硫胺素(维生素B₁)由嘧啶和噻唑部分通过化学偶联产生。核黄素(维生素B₂)由鸟苷-5’-三磷酸(GTP)和核糖-5’磷酸合成。核黄素接下来用于合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。合起来称为‘维生素B₆’的化合物类(例如，吡哆素、吡哆胺、5’-磷酸吡哆醛和商业上使用的维生素B₆盐酸盐)均为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸(R)-(+)-N-(2，4-二羟基-3，3-二甲基-1-氧代丁基)-β-丙氨酸)可通过化学合成或发酵产生。泛酸生物合成的最后步骤由ATP-驱动的β-丙氨酸和泛解酸的缩合组成。负责泛解酸和β-丙氨酸转化及泛酸缩合生物合成步骤的酶是已知的。泛酸盐的代谢活性形式为辅酶A，其生物合成由5步酶促步骤进行。泛酸盐、5’-磷酸吡哆醛、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸盐的形成，而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(维生素B₅原)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。

已详细研究了微生物中生物素从前体分子庚二酰辅酶A的生物合成，并鉴定了几种所涉及的基因。发现许多相应的蛋白质也参与Fe-簇合成，属于蛋白质nifS类。硫辛酸衍生自辛酸，并在能量代谢中作为辅酶，此时其成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸盐为一组这样的物质，其均为叶酸衍生物，而叶酸来自于L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已详细研究了某些微生物中始于鸟苷-5’-三磷酸(GTP)的生物转化的代谢中间体、L-谷氨酸和对氨基苯甲酸的叶酸及其衍生物的生物合成。

类可啉(如钴胺素且尤其是维生素B₁₂)和卟啉属于一组特征在于四吡咯环体系的化学品。维生素B₁₂的生物合成太复杂，目前尚未彻底解释清楚，但许多涉及的酶和底物现已知。烟酸(烟酸盐)和烟酰胺为吡啶衍生物，也称为‘烟酸(niacin)’。烟酸为重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)和它们的还原形式的前体。

这些化合物的工业规模的生产基本上依赖于无细胞的化学合成，虽然这些化学品中的一些如核黄素、维生素B₆、泛酸盐和生物素也已通过微生物的大规模培养物产生。只有维生素B₁₂因为其合成的复杂性是仅通过发酵产生的。体外方法需要支出相当大量的材料和时间，并常常代价很高。

C.嘌呤、嘧啶、核苷和核苷酸的代谢和用途

嘌呤和嘧啶代谢基因及它们相应的蛋白质为治疗肿瘤和病毒感染的重要靶标。术语“嘌呤”或“嘧啶”包括构成核酸、辅酶和核苷酸的含氮碱基。术语“核苷酸”包括核酸分子的基本结构单元，其包括含氮碱基、戊糖(对RNA而言，该糖为核糖，对DNA而言，该糖为D-脱氧核糖)和磷酸。术语“核苷”包括作为核苷酸前体的分子，但其不含核苷酸所具有的磷酸单元。通过抑制这些分子的生物合成，或抑制它们形成核酸分子的动员，可抑制RNA和DNA合成；通过以靶向癌细胞的方式抑制这种活性，可抑制肿瘤细胞分裂和复制的能力。

此外，有不形成核酸分子但作为能量贮存体(即AMP)或作为辅酶(即FAD和NAD)的核苷酸。

一些出版物已描述了这些化学品通过影响嘌呤和/或嘧啶代谢在这些医学适应症中的用途(例如Christopherson，R.I.和Lyons，S.D.(1990)”作为化学治疗剂的嘧啶和嘌呤从头生物合成的强力抑制剂”医学研究述评(Med.Res.Reviews)10：505-548)。对参与嘌呤和嘧啶代谢的酶的研究集中在研发可用作如免疫抑制剂或抗增生剂的新药上(Smith，J.L.，(1995)”核苷酸合成中的酶”，结构生物学最新观点(Curr.Opin.Struct.Biol.)5：752-757；(1995)Biochem Soc.Transact.23：877-902)。但是，嘌呤和嘧啶碱基、核苷和核苷酸具有其它可能应用：用作多种精细化学品(例如，硫胺素、S-腺苷-甲硫氨酸、叶酸盐或核黄素)生物合成的中间体，作为细胞的能量载体(例如，ATP或GTP)，和本身作为化学品，通常用作增味剂(例如，IMP或GMP)或用于一些医药用途(参见例如，Kuninaka，A.(1996)生物技术中的核苷酸和相关化合物(Nucleotides and Related Compounds inBiotechnology)第6卷，Rehm等人编辑.VCH：Weinheim，561-612页)。同样，参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作靶，研发用于作物保护的抗这些酶的化学品，包括杀真菌剂、除草剂和杀虫剂。

已阐释了这些化合物在细菌中的代谢(综述参见例如，核酸研究和分子生物学进展(Progress in Nucleic Acid Research and Molecular Biology)，第42卷，Academic Press中的Zalkin，H.和Dixon，J.E.(1992)”嘌呤核苷酸的从头生物合成”，259-287页；和生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：纽约一书中的Michal，G.(1999)”核苷酸和核苷”，第8章)。嘌呤代谢已是深入研究的主题，其对细胞的正常功能是必需的。高等动物中受损的嘌呤代谢可引起严重疾病，如痛风。嘌呤核苷酸从核糖-5-磷酸开始，经过一系列步骤，通过中间体化合物次黄苷-5’-磷酸(IMP)，产生鸟苷-5’-单磷酸(GMP)或腺苷-5’-单磷酸(AMP)，由此可以容易地形成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存体，这样它们的降解可为细胞内许多不同生物化学过程提供能量。嘧啶的生物合成由核糖-5-磷酸形成尿苷-5’-单磷酸(UMP)而进行。UMP接下来转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式通过一步还原步骤从核苷酸的二磷酸核糖形式产生为核苷酸的二磷酸脱氧核糖形式。通过磷酸化后，这些分子可参与DNA合成。

D.海藻糖的代谢和用途

海藻糖由两个葡萄糖分子通过α、α-1，1键连接组成。它通常作为甜味剂用于食品工业，或作为干的或冷冻食品和饮料中的添加剂。但它也用于药学、化妆品和生物技术工业(参见例如，Nishimoto等人(1998)美国专利号5,759,610；Singer，M.A.和Lindquist，S.生物技术趋势(TrendsBiotech.)16(1998)：460-467；Paiva，C.L.A.和Panek，A.D.Biotech.Ann.Rev.2(1996)：293-314；和Shiosaka，M.J.Japan 172：(1997)97-102)。海藻糖可以通过许多微生物的酶产生，并天然释放入环境介质中，从中可用本领域公知的方法分离海藻糖。

II.谷氨酸棒状杆菌中遗传稳定性、蛋白质合成和蛋白质分泌

细胞如谷氨酸棒状杆菌中目标化合物的生产是许多分开的但是仍然相互关联的过程的终点，这些过程的每一个都对所述化合物总的生产和从细胞中的释放至关重要。当修饰细胞使其过量生产一种或多种化学品时，必须考虑这些过程的每一个以保证细胞的生物化学机器与该遗传操作相容。尤其重要的细胞机制包括导入细胞中的修饰基因的稳定性、突变基因被正确转录和翻译的能力(包括密码子使用)和突变蛋白产物被正确折叠和/或分泌的能力。

A.细菌修复和重组系统

细胞不断暴露于核酸损伤物质如紫外辐射、氧自由基和烷基化中。此外，甚至DNA聚合酶的作用也会出错。细胞必须在遗传稳定性(这保证细胞功能所需的基因在正常生长和代谢中不受损伤)和遗传变异(这使得细胞可以适应不断变化的环境)间保持平衡。所以，大多数细胞含有分开的DNA修复和DNA重组途径，然而这两个途径又互相联系。DNA修复途径通过直接逆转损伤或者通过切除被损伤的区域并用正确的序列代替来严格地纠正DNA分子中的错误。DNA重组系统也修复核酸分子，但是只是修复那些在两条DNA链中都受损而不可能用任何一条作为模板修复另一条的损伤。重组修复和SOS反应可容易地导致在受损区内或附近产生倒位、缺失或其他遗传重排，这反过来促进某种程度的基因组不稳定性，这可有助于细胞适应不断改变的环境或胁迫。

高保真性的修复机制包括直接逆转DNA损伤和切除所述损伤并用互补链中编码的信息重新合成。所述损伤的直接逆转需要一种具有可导致与原来损伤DNA的活性相反的活性的酶。例如，DNA修复甲基转移酶能够纠正错误的DNA甲基化，脱氧核糖二嘧啶光解酶能够修复紫外辐射产生的核苷酸二聚体，该酶在光的存在下将所述二聚体切割成相应的核苷酸完成修复(见Michal，G.(1999)生物化学途径：生物化学和分子生物学地图集(Biochemistry Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：纽约，及其参考文献)。

准确修复大的损伤需要专门的修复机制。这些机制包括错配修复和切除修复系统。单个碱基的损伤可以通过多个切割反应纠正：糖键首先被切开，然后在损伤位点切开DNA主链和除去受损的碱基。最后DNA聚合酶和DNA连接酶用第二DNA链作为模板补平并关闭缺口。更为实质性的导致双螺旋构象改变的DNA损伤通过ABC系统纠正，在ABC系统中，解旋酶II、DNA聚合酶I、UvrA、UvrB和UvrC蛋白一起在受损位点切割双螺旋的单链，以依赖ATP的方式解开受损区，切除受损区并用另一条链作为模板填补丢失的区域。最后，DNA连接酶关闭该单链断裂。对G-T错配(Vsr蛋白参与该修复系统)和由于两条链的错误修复造成的小的缺失/插入错误(甲基化控制的途径参与该修复系统)也有专门的修复系统。

还有低保真性修复系统，它们通常用于纠正细菌中非常长的DNA损伤。对于影响两条DNA链的损伤进行双链修复和重组。在该情况下，不可能通过使用另一链作为模板修复该损伤。从而，该修复系统包括损伤区域和同源DNA分子上该区域的另一拷贝间的双交换事件。这是可能的，因为细菌分裂得如此之快，在细胞分裂实际发生之前，基因组DNA的第二份拷贝通常是可以得到的。所述交换事件可容易地导致倒位、复制、缺失、插入和其他遗传重排，从而总体上增加了该生物的遗传不稳定性。

如果DNA中的损伤足够使DNA聚合酶III停止而不能继续复制，那么SOS反应就被激活。在这些情况下存在单链DNA。RecA蛋白通过结合单链DNA而被活化，该活化的形式导致LexA阻遏物的激活，从而解除20多个基因(包括UvrA、UvrB、UvrC、解旋酶II、DNA pol III、UmuC、UmuD)的转录阻滞。这些酶的组合活性可充分填补缺口区以致DNA polIII继续复制。然而，这些缺口会被填充上本不存在的碱基，从而这种类型的修复导致易于出错的修复，这总体上促进了细胞的遗传不稳定性。

B.转座子

上面提高的高或低保真性系统可以修复DNA损伤。但在某些情况下，该修复可包括额外的基因重排。而且，许多细菌细胞具有专门导致这种基因重排的机制。这些机制中尤其熟知的例子就是转座子。

转座子是能从一个位点迁移到另一个位点的遗传元件，它可以在染色体内或者染色体外DNA(例如质粒)和染色体之间迁移。可以以几种途径进行转座；例如，转座因子可以从供体位点切除并整合到靶位点(非复制型转座)，或者备选地，可从供体位点复制到靶位点，导致所述元件的两个拷贝(复制型转座)。供体位点和靶位点的序列通常不相关。

所述转座事件具有多种可能的结果。转座因子在一个基因中的整合可以破坏所述基因，这通常完全去除了所述基因的功能。在所述基因的周围DNA中发生的整合不会干扰编码序列自身，但是可以对所述基因的调节及其表达具有重要影响。位于基因组不同部分的两个转座因子拷贝间的重组事件可导致基因组片段的缺失、复制、倒位、转座或扩增。各种复制子也可能融合。

最简单的类似转座子的遗传元件称作插入(IS)元件。IS元件含有一个可变长度(但是通常小于1500个碱基)的核苷酸区，其不含有编码区但在每个末端被反向重复序列包围。由于IS元件不编码任何可检测到活性的蛋白，通常观测到IS元件的存在只是因为IS元件插入的一个或多个基因丧失了功能。

转座子是可移动的遗传元件，和IS元件相比，转座子含有与重复序列为边界的核苷酸序列，并且该序列可以编码一种或多种蛋白。这些重复区常含有IS元件。转座子编码的蛋白通常是转座酶(催化转座子从一个位点迁移到另一个位点的蛋白)和抗生素抗性基因。转座因子的机制和调节是本领域公知的并且描述于(至少通过例子)：Lengeler等(1999)原生生物生物学(Biology of Prokaryotes)，Thieme Verlag：Stuttgart，375-361页；Neidhardt等(1996)大肠杆菌和沙门氏菌(Escherichia coli andSalmonella)，ASM出版社：华盛顿特区；Sonenshein，Al.L.，等，编辑，(1993)枯草芽孢杆菌(Bacillus subtilis)，ASM出版社，华盛顿特区；Voet，D.，和Voet，J.G(1992)Biochemie，VCH：Weinheim，985-990页；Brock，T.D.，和Madigan，M.T.(1991)微生物生物学(Biology ofMicroorganisms)，第6版，Prentice Hall：纽约，267-269页；和Klechner，N.(1990)“细菌中转座的调节”，Annu.Rev.Biochem.61：297-327。

C.转录

细菌中基因的表达主要在转录水平被调节。转录装置包括许多蛋白，可将它们分成两组：RNA聚合酶(操纵DNA转录的酶)和σ因子(通过将RNA聚合酶指向识别所述因子的特定启动子DNA序列来调节基因转录)。RNA聚合酶和σ因子的组合产生了RNA聚合酶全酶——一种活化的复合体。革兰氏阳性细菌如棒状杆菌只含有一种类型的RNA聚合酶但是合有许多不同σ因子，它们对不同启动子、生长期、环境条件、底物、氧气水平、运输过程等特异，结果，该生物能够适应各种环境和代谢条件。

启动子是作为RNA聚合酶全酶停靠位点的特定DNA序列。许多启动子元件具有保守序列元件，它们可以通过同源搜索检测；备选地，可以用标准技术如引物延伸来鉴定特定基因的启动子区域。公知许多革兰氏阳性细菌的启动子区(见，例如Sonenshein，A.L.，Hoch，J.A.，和Losick，R.，编辑，(1993)枯草芽孢杆菌，ASM出版社：华盛顿特区)。

许多阻遏或活化机制影响启动子的转录控制。结合启动子的特定调节蛋白能够阻止(阻遏物)或支持(激活物)RNA全酶的结合，从而调节转录。这些阻遏物或激活物分子的结合也受到它们与其他分子如蛋白或其他代谢化合物的相互作用的调节。备选地，转录可以受到影响如延伸或终止等过程的因子的调节(见，例如Sonenshein，A.L.，Hoch，J.A.，和Losick，R.，编辑，(1993)枯草芽孢杆菌，ASM出版社：华盛顿特区)。

对各种环境或代谢信号作出反应调节基因的转录的能力使得细胞能够准确控制什么时候表达基因以及某时刻细胞中能存在多少基因产物。这反过来防止了不必要的能量浪费或者不必要的可能是稀有的中间物或辅因子的使用。

D.翻译和氨酰tRNA合成酶

翻译是根据RNA分子中所含信息从氨基酸合成多肽的过程。该过程的主要组分是核糖体与特异起始或延伸因子，如IF1-3、INVENTIVE-G和EFTu(见，例如Sonenshein，A.L.，Hoch，J.A.，和Losick，R.，编辑，(1993)枯草芽孢杆菌，ASM出版社：华盛顿特区)。

mRNA分子的每个密码子编码一个特定氨基酸。通过转移-RNA(tRNA)将mRNA转变成氨基酸。这些tRNA分子由一条RNA单链(60到100个碱基之间)组成，该单链以L形三维结构存在，具有延伸区或“手臂”。这些手臂中的一个与mRNA分子上的特定密码子序列形成碱基对。另一条手臂特异性地与特定氨基酸(由密码子编码)相互作用。其他tRNA手臂包括可变手臂、TψC手臂(其携带胸苷酸和假尿苷酸修饰)和D手臂(其携带二氢尿苷修饰)。还不清楚所述其他tRNA结构的功能，但是它们在tRNA分子中的保守性暗示它们在蛋白合成中有作用。

为了基于核酸的tRNA分子与正确氨基酸配对，称作氨酰-tRNA合成酶的一类酶必须起作用。存在多种多样的这种酶，它们中的每一个都对特定tRNA和特定氨基酸特异。所述酶在一两步反应中将末端tRNA-腺苷-核糖单位的3’-羟基结合到氨基酸。首先，该酶通过与ATP和氨基酸反应而活化，形成氨酰-tRNA-合成酶-氨酰-腺苷酸复合体。其次，氨酰基基团从酶转移到目标tRNA上，氨酰基在目标tRNA上保持高能状态。然后tRNA分子与其在mRNA分子上的识别密码子的结合使tRNA-结合的高能氨基酸与核糖体接触。在核糖体中，装载氨基酸的tRNA(氨酰基tRNA)占据结合位点(A位点)，其旁边的另一个位点(P位点)携带氨基酸与新生多肽链结合的tRNA分子(肽酰tRNA)。氨酰基tRNA上的活化氨基酸具有充分反应性，使得在该氨基酸和新生多肽链上的邻近氨基酸之间自发形成肽键。GTP水解为转移tRNA提供能量，tRNA此时装载多肽链从核糖体的A位点转移到P位点，该过程重复进行直到遇到终止密码子。

翻译可以在许多不同步骤上被调节。这些步骤包括核糖体与mRNA的结合、mRNA二级结构的存在、密码子使用或特定tRNA的频率。特殊调节机制如衰减作用也可以在翻译水平起作用。可以在例如Vellanoweth，R.L.(1993)《枯草芽孢杆菌和其他革兰氏阳性细菌》中的“翻译及其调节”，Sonenshein，A.L.，等，编辑，ASM出版社：华盛顿特区，699-711及其中所引用的参考文献中发现许多这些机制的深入讲解。

E.蛋白质折叠和蛋白质分泌

蛋白质的核糖体合成形成的多肽链必须采用三维形式才能够正常发挥功能。通过折叠过程形成三维结构。多肽链具有柔性并且(原则上)容易在溶液中自由移动，直到它们采用一种导致更稳定的三维结构的构象。然而，有时蛋白质折叠是困难的，这或者是因为环境条件(例如，高温下系统中的动力学能量使得蛋白更难达到能量最小化的稳定结构)或者是因为蛋白质本身的类型(例如蛋白质中彼此紧靠的疏水区易于聚集，从而使它们从水性溶液中沉淀下来)。

已经鉴定了能够催化、陪伴或者支持蛋白质折叠的类似蛋白质的因子，它们被共翻译或翻译后合成。这些蛋白质折叠分子包括脯氨酰-肽基异构酶(例如引发因子、cyclophilin和FKBP同系物)以及热休克蛋白(例如DnaK、DnaJ、GroEL、小热休克蛋白、HtpG和Clp家族成员(例如ClpA、ClpB、ClpW、ClpP和ClpX)。这些蛋白中的许多对细胞的存活重要：除了它们在蛋白质折叠、蛋白质运输和蛋白质加工中的作用，它们还常常作为蛋白质合成总体调节的靶标(见，例如Bukau，B.(1993)分子微生物学(Molecular Microbiology)9(4)：671-680；Bukau，B.，和Horwich，A.L.(1998)细胞(Cell)92(3)：351-366；Hesterkamp，T.，Bukau，C.(1996)FEBS Lett.389(1)：32-34；Yaron，A.，Naider，F.(1993)生物化学和分子生物学的关键评论(Critical Reviews in Biochemistry and MolecularBiology)28(1)：31；Scheibel，R.，Buchner，J.(1998)生物化学药理学(Biochemical pharmacology)56(6)：675-682；Ellis，R.J.，Hartl，F.U.t1996)FASEB Journal 10(1)：20-26；Wawrzynow，A.，等(1996)分子微生物学(Molecular Microbiology)21(5)：895-899；Ewalt，K.I.，等(1997)细胞90(3)：491-500)。

前面鉴定的伴侣蛋白以两种方式起作用：它们或者结合并稳定多肽或者提供折叠可发生而不被破坏的环境。前者包括例如DnaK、DnaJ和热休克蛋白，它们直接结合新生或错误折叠的多肽，并常伴随着ATP水解。伴侣蛋白的结合防止了多肽与其他多肽的聚集，并且能够迫使已经形成的团聚体解体。与另一伴侣蛋白GrpE相互作用(这使得可能发生ADP-ATP互换)后，多肽在熔球态被释放出来并能够折叠。如果折叠错误，伴侣蛋白再次结合错误折叠的蛋白并且迫使其返回到未折叠的状态。该循环可以反复发生直到蛋白正确折叠。与简单结合多肽的第一组伴侣蛋白相比，第二组(例如GroEL/ES)不仅结合多肽，而且完全将其围住使多肽免受环境影响。GroEL/ES复合体由具有疏水内表面的两个堆叠的14元环和一个7元环构成的“盖子”组成。在依赖ATP的反应中，多肽被牵引到该复合体的中心通道中，在该通道中多肽能够折叠而不受其他多肽的破坏。错误折叠的蛋白不会从该复合体释放。

蛋白质折叠中一个重要的步骤是二硫键的形成。这些键存在于亚基中或者蛋白质的亚基之间，它们对蛋白质的稳定性是重要的。在水性溶液中易于形成二硫键，在没有还原环境的帮助下较难逆转错误的二硫桥形成。为了支持正确的二硫桥形成过程，大多数细胞的胞质溶胶含有含巯基的分子，如谷胱甘肽或硫氧还蛋白和它们相应的氧化/还原系统(Loferer，H.，Hennecke，H.(1994)生物化学科学的趋势(Trends in BlochemicalSciences)19(4)：169-171)。

然而，在某些时候并不需要新生多肽折叠，例如如果这些蛋白将要被分泌的时候。折叠过程通常导致蛋白的疏水区域位与所述蛋白的中心，从水性溶液中脱离出来，并且亲水区域被呈现在所述蛋白的外部表面。

尽管这种构象排列产生了稳定性较高的蛋白，但是它使得该蛋白跨膜运输更困难，因为膜的疏水核心本身与蛋白的亲水外层不相容。从而，由细胞合成的必须被分泌到细胞外面的蛋白(例如细胞表面酶和膜受体)或者必须插入到膜自身中的蛋白(例如转运蛋白和通道蛋白)通常在折叠前分泌或插入。防止新生多肽链聚集的相同伴侣蛋白也防止多肽的折叠，直到不再需要它们。因此，这些蛋白能够“护卫”新生多肽链到细胞中的合适位置，在此它们或者被除去以便蛋白能够折叠或者将蛋白运送到转运系统，该系统或者将多肽分泌出去或者支持其插入到膜中。

在进化过程中形成了一种专门的蛋白机器，其识别、结合、运输和加工具有特定前序列(它们后来通过切割从该蛋白中除去)的蛋白。所述机器包含总称为sec(II型分泌)系统的许多蛋白(回顾见Gilbert，M.，等(1995)生物技术关键回顾(Critical Reviews in Biotechnology)15(1)：13-39及其参考文献；Freudl，R.(1992)生物技术杂志(Journal of Biotechnology)23(3)：231-240及其参考文献；Neidhardt，F.C.，等(1996)大肠杆菌和沙门氏菌(E.coli and Salmonella)，ASM出版社：华盛顿特区，967-978页；Binet，R.，等(1997)Gene 192(1)：7-11 und Rapoport，T.A.(1986)生物技术关键回顾(Critical Reviews in Biochemistry)20(1)：73-137及其参考文献)。Sec系统包含伴侣蛋白(例如SecA和SecB)、整合膜蛋白(也称作移位酶(例如SecY、SecE和SecG))信号肽酶(例如LepB)。具有导致分泌的前序列的新生多肽与SecB结合，SecB将其转移到细胞膜内表面上的SecA。SecA结合前序列，ATP水解后，插入到膜中并迫使部分多肽穿过膜。多肽剩余部分通过移位酶(如SecY、SecE和SecG)复合物被引导穿过膜。最后，信号肽酶通过切割除去前序列，多肽游离定位在膜的胞外面并在那儿自发折叠。

独立于Sec的分泌机制也是公知的。例如，依赖信号识别颗粒的途径包括在新生多肽合成中信号识别颗粒(SRP)蛋白结合该新生多肽，从而使核糖体停止。然后，膜内表面的SRP受体结合核糖体-多肽-SRP复合体。GTP水解提供了将此复合体转移到sec-移位酶复合体所需的能量，在sec-移位酶复合体上，该多肽在其被核糖体合成的过程中被引导穿过膜。公知存在其他分泌机制，这些机制只是对少许蛋白特异。

III.本发明元素和方法

本发明至少部分是基于新分子的检测，这些分子在本文中称作SES核酸和SES蛋白分子，它们参与谷氨酸棒状杆菌中DNA的修复或重组、谷氨酸棒状杆菌DNA的转座或其它重排、谷氨酸棒状杆菌中基因的表达(即转录或翻译过程)以及该微生物的蛋白质折叠或蛋白质分泌。在一个实施方案中，SES分子参与谷氨酸棒状杆菌中DNA的修复或重组、遗传物质的转座、基因表达(即，转录或翻译过程)、蛋白质拆叠或蛋白质分泌。在一个优选的实施方案中，本发明的SES分子的活性通过影响DNA的修复或重组、DNA的转座、基因表达、蛋白质折叠或蛋白质分泌而影响所述微生物的目标精细化学品的生产。在一个尤其优选的实施方案中，本发明SES分子的活性受到调节，使得本发明SES参与的谷氨酸棒状杆菌的细胞过程(例如DNA的修复或重组、DNA转座、基因表达、蛋白质折叠或蛋白质分泌)的活性也被改变，这直接或间接地导致谷氨酸棒状杆菌生产目标精细化学品的产率、产量和/或效率受到调节。

术语“SES蛋白”或“SES多肽”包括参与许多与谷氨酸棒状杆菌中的遗传稳定性、基因表达、蛋白质折叠或蛋白质分泌相关的细胞过程的蛋白质。例如，SES蛋白可参与谷氨酸棒状杆菌中DNA修复或重组机制，参与谷氨酸棒状杆菌的遗传物质的重排(如那些受转座子介导的重排)，参与该生物中基因的转录或翻译，参与介导谷氨酸棒状杆菌中蛋白质的折叠(如伴侣蛋白活性)或者参与谷氨酸棒状杆菌细胞的蛋白质的分泌(例如在sec系统上)。SES蛋白的例子包括那些由表1和集合A中列出的SES基因编码的蛋白。术语“SES基因”或“SES核酸序列”包括编码SES蛋白的核酸序列，其包含编码区和相应的非翻译的5’和3’序列区。SES基因的实例在表1中列出。

术语“产量”和“生产力”是本领域中已知的并且包括发酵产物的浓度(例如，在预定的时间段和预定的发酵体积内生产的目标精细化学品的浓度(例如kg产物/h/l))。术语“生产效率”包括细胞为得到特定产物量所需的时间(例如，细胞为达到精细化学品的特定产出率所需的时间)。术语“产率”或“产物/碳产率”是本领域已知的并且包括将碳源转化成产物(即精细化学品)的效率。这例如通常表达为kg产物/kg碳源。增加化合物的产率或产量可在预定时间内在特定培养体积中增加所得分子或该化合物的适宜得到的分子的量。术语“生物合成”和“生物合成途径”是本领域中已知的并且包括细胞在例如多步过程或高度调节的过程中从中间物合成化合物，优选有机化合物。术语“降解”和“降解途径”是本领域中已知的并且包括细胞在例如多步过程或高度调节的过程中将化合物，优选有机化合物切割成降解产物(用更通俗的术语：更小或更简单的分子)。术语“代谢”是本领域中已知的并且包括生物体中发生的生物化学反应的整体。这样，特定化合物的代谢(例如氨基酸如甘氨酸的代谢)包括细胞中与该化合物有关的所有生物合成、修饰和降解途径。

术语“DNA修复”是本领域公知的，包括将DNA中的错误(或者由于损伤如，但不限于，紫外辐射、甲基化酶、低保真性复制或诱变剂)切除和纠正的细胞机制。术语“重组”或“DNA重组”是本领域公知的，包括这样的细胞机制，该机制可以通过与同一细胞中另一份未损伤的DNA分子拷贝同源重组来纠正影响DNA分子的两条链的大的DNA损伤。这些修复通常是低保真性的，并且可以导致基因重排。术语“转座子”是本领域公知的，包括一种DNA元件，其能够随机插入生物体基因组并且可以导致基因或它们的调节区被破坏或者导致复制、倒位、缺失和其他基因排列。术语“蛋白质折叠”是本领域公知的，包括多肽链在几种三维构型间变动直到达到稳定的活性三维构型。二硫键的形成和疏水区从周围水性溶液的分离为该蛋白的折叠过程提供了一定的动力，并且伴侣蛋白的活性可以加强正确折叠。术语“分泌”或者“蛋白质分泌”是本领域中公知的，包括蛋白质以某种机制从细胞内部移动到细胞外部，在该机制中分泌蛋白系统使得蛋白穿过细胞膜到达细胞外面。

在另一个实施方案中，本发明的SES分子能够调节目标分子，如精细化学品在微生物如谷氨酸棒状杆菌中的生产。存在多种机制，通过这些机制本发明的SES蛋白的修饰能够直接影响含有该修饰蛋白的谷氨酸棒状杆菌菌株的精细化学品生产的产率、产量和/或效率。例如，调节直接参与转录或翻译的蛋白(例如聚合酶或核糖体)使它们的数量或者活性增加将总体上增加细胞的转录或翻译(或者这些过程的速率)。这种增加的细胞基因表达将包括那些参与精细化学品生物合成的蛋白，使得能够增加一种或多种目标化合物生产的产率、产量或效率。修饰谷氨酸棒状杆菌转录/翻译蛋白机器以改变这些蛋白的调节也能增加参与精细化学品生产的基因的表达。对许多参与肽折叠的蛋白活性的修饰可以增加细胞中正确折叠分子的总体生产，从而增加目标蛋白(例如精细化学品生物合成蛋白)正确发挥功能的可能性。此外，可能可以通过突变参与谷氨酸棒状杆菌分泌的蛋白以增加它们的数量或活性，使精细化学品(例如酶)从细胞向发酵培养基中的分泌增加，而从发酵培养基中可以容易地得到所述精细化学品。

本发明的SES分子的遗传修饰也能够间接调节一种或多种精细化学品的生产。例如，可以通过增加本发明的DNA修复蛋白或DNA重组蛋白的数量或活性来增加细胞检测并修复DNA损伤的能力。这将有效增加细胞将突变基因保留在其基因组中的能力，从而增加通过遗传方法导入谷氨酸棒状杆菌中的转基因(其编码例如可以增加精细化学品生物合成的蛋白)在微生物的培养中不被丢失的可能性。相反，可以通过减少一种或多种DNA修复蛋白或DNA重组蛋白的数量或活性来增加生物的遗传不稳定性。这些操作将提高所述生物通过诱变被修饰而不纠正导入的突变的能力。对于参与谷氨酸棒状杆菌中遗传元件的转座或重排的蛋白(例如转座子)，这也是一样的。增加或减少这些蛋白的数量或活性的突变发生使得可能同时增加和减少微生物的遗传稳定性。这关键性地影响向谷氨酸棒状杆菌中导入另一个突变和保留导入的突变的可能性。转座子同样提供了一种诱变谷氨酸棒状杆菌的合适的机制；通过转座子诱变可以容易地进行目标基因(例如精细化学品的生物合成基因)的复制以及不想要的基因(例如参与目标精细化学品的降解的基因)的破坏。

可以通过调节一种或多种参与对特定环境条件应答而调节转录或翻译的蛋白(例如，σ因子)来防止细胞在存在于大规模发酵培养中的不利的环境条件下减慢或者停止蛋白质合成。这将增加基因表达，并且这反过来可以使目标精细化学品在所述条件下的生物合成增加。许多这样的分泌蛋白具有对细胞存活重要的功能(例如细胞表面蛋白酶或细胞表面受体)。通过改变分泌途径使所述蛋白更容易被运输到它们的胞外位置，可以增加细胞总体存活力从而导致在大规模发酵时存在更多能够生产精细化学品的谷氨酸棒状杆菌细胞。因为还知道特定细菌蛋白分泌途径(例如sec系统)也参与整合膜蛋白(例如受体、通道、孔或转运蛋白)向膜的插入，故调节参与谷氨酸棒状杆菌蛋白质分泌的蛋白的活性可影响细胞清除废产物或者输入必需代谢物的能力。如果这些分泌性蛋白的活性增加，那么细胞生产精细化学品的能力(由于膜中存在增多的转运蛋白/通道，它们能够输入营养物或清除废产物)同样也可以增加。如果所述分泌蛋白的活性降低了，可能将没有足够营养物来支持目标化合物的过量生产或者废产物将干扰该生物合成。

制备本发明的核酸序列的一个适宜的起点是谷氨酸棒状杆菌菌株的基因组，该菌株可以从美国典型培养物保藏中心获得，其名称是ATCC13032。

可以使用常用方法通过表1中所示的修饰从这些核酸序列制备本发明的核酸序列。

本发明的SES蛋白或其生物学活性部分或片段可以参与谷氨酸棒状杆菌中DNA修复或重组、遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌或者具有表1中描述的一种或多种活性。

下面的小章节更详细地描述了本发明的各个方面：

A.分离的核酸分子

本发明的一个方面涉及编码SES多肽或其生物活性部分的分离核酸分子及核酸分子片段，其中所述核酸分子片段足以用作杂交探针或引物，用来鉴定或扩增编码SES的核酸(例如SES DNA)。如此处所用，术语“核酸分子”是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)和用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’和5’端的非翻译区：基因编码区5’端上游的至少约100个核苷酸的序列和基因编码区3’端下游的至少约20个核苷酸的序列。核酸分子可为单链或双链，但优选地为双链DNA。“分离的”核酸分子为从存在于此核酸的天然源中的其它核酸分子中取出的核酸分子。优选地，“分离的”核酸不具有在该核酸所来自的生物体基因组DNA中天然存在于该核酸侧翼的序列(例如，位于该核酸5’或3’端的序列)。例如，在不同实施方案中，该分离的SES核酸分子可包含少于约5kb，4kb，3kb，2kb，1kb，0.5kb或0.1kb在该核酸所来自的细胞(如谷氨酸棒状杆菌细胞)基因组DNA中天然存在于该核酸分子侧翼的核苷酸序列。而且，“分离的”核酸分子如cDNA分子当通过重组技术产生时可基本上不含有其它细胞物质或培养基，当通过化学合成产生时，可基本上不含有化学前体或其它化学品。

本发明的核酸分子，如具有集合A的核苷酸序列的核酸分子或其部分可用标准的分子生物学技术和本文提供的序列信息制备。例如，使用集合A的完整或部分序列作为杂交探针和标准的杂交技术(例如，在Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，冷泉港实验室，冷泉港实验室出版社，冷泉港，纽约，1989中所述)，可自谷氨酸棒状杆菌文库分离谷氨酸棒状杆菌SES cDNA。而且，包含集合A的完整序列或其部分的核酸分子可使用根据该序列设计的寡核苷酸引物，应用聚合酶链反应分离(例如，包含集合A的完整或部分序列的核酸分子可使用根据该同样序列设计的寡核苷酸引物，应用聚合酶链反应分离)。例如，可从正常的内皮细胞分离mRNA(例如，通过Chirgwin等人(1979)生物化学(Biochemistry)18：5294-5299的硫氰酸胍提取法)并用反转录酶(例如，莫洛尼鼠类白血病病毒反转录酶，可从Gibco/BRL，Bethesda，MD获得；或AMV反转录酶，可从SeikagakuAmerica，Inc.，St.Petersburg，FL获得)制备cDNA。用于聚合酶链反应扩增的合成寡核苷酸引物可根据集合A所述的核苷酸序列之一设计。本发明的核酸可用cDNA或用基因组DNA作为模板、使用合适的寡核苷酸引物按照标准的PCR扩增技术来扩增。这样扩增的核酸可克隆入合适的载体并通过DNA序列分析鉴定。对应于SES核苷酸序列的寡核苷酸还可以通过标准的合成技术制备，例如用DNA自动合成仪制备。

在一个优选的实施方案中，本发明的分离核酸分子包含集合A中所示的核苷酸序列之一。

在另一个优选的实施方案中，本发明的分离的核酸分子含有与集合A中所示核酸序列之任一互补的核酸分子或其部分，所述核酸分子与集合A中所示核酸序列之任一足够互补以致其与集合A中所示序列之一能杂交而得到稳定双链体。

在一个实施方案中，本发明的核酸分子编码这样的蛋白质或其部分，其包括充分同源于集合B的氨基酸序列的氨基酸序列，以至于该蛋白质或其部分仍然能够参与谷氨酸棒状杆菌中DNA的修复或重组，遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌。如此处所用，术语“充分同源”是指蛋白质或其部分所具有的氨基酸序列包括最小数目的与集合B的氨基酸序列相同或等同的氨基酸残基(例如，氨基酸残基具有与集合B中序列之一的氨基酸残基相似的侧链)，以至于该蛋白质或其部分能够参与谷氨酸棒状杆菌中DNA的修复或重组，遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌。如此处所述的谷氨酸棒状杆菌中参与遗传稳定性、基因表达、蛋白质折叠或蛋白质分泌的蛋白可能在一种或多种精细化学品的生产和分泌中起作用。此类活性的实例也在此进行了描述。因此，“SES蛋白的功能”对一种或多种精细化学品的产率、产量和/或生产效率起直接或间接的作用。SES蛋白的实例在表1中给出。

本发明SES核酸分子编码的蛋白的部分优选是任何SES蛋白的具生物学活性的部分。文中所用术语“SES蛋白的生物学活性部分”旨在包括SES蛋白的一部分，例如结构域或基元，其能够参与谷氨酸棒状杆菌中DNA的修复或重组，遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌或者具有表1中所示任一活性。为了确定SES蛋白或者其生物学活性部分能否参与谷氨酸棒状杆菌中DNA的修复或重组，遗传物质的转座、基因表达(即转录或翻译过程)、蛋白质折叠或蛋白质分泌，可以进行酶活性分析。这些分析方法在实施例部分的实施例8中详细描述，技术人员熟知这些方法。

除了群体中可能存在的SES序列的天然变体外，技术人员还理解可通过突变向集合A的核苷酸序列中导入变化，由此导致所编码的SES蛋白的氨基酸序列改变，但不损害SES蛋白的功能。例如，可在集合A的核苷酸序列中进行在“非必需”氨基酸残基处导致氨基酸替换的核苷酸替换。“非必需”氨基酸残基是任一野生型SES蛋白序列(集合B)中可以被修饰而不改变所述SES蛋白的活性的残基，但“必需”氨基酸残基为SES蛋白活性所需。然而，其它氨基酸残基(例如，在具有SES活性的结构域中不保守或仅为半保守的那些氨基酸残基)对活性可能为非必需的，并因此可能被改变而不导致SES活性的改变。

编码同源于集合B的蛋白质序列的SES蛋白的分离核酸分子可通过将一个或多个核苷酸替换、添加或缺失导入集合A的核苷酸序列中以使一个或多个氨基酸替换、添加或缺失导入所编码的蛋白质中而产生。可通过标准技术将突变导入集合A的核苷酸序列之一，如定点诱变和PCR-介导的诱变。优选地，在一处或多处预测的非必需氨基酸残基处进行保守氨基酸替换。“保守氨基酸替换”为将氨基酸残基用具有相似侧链的氨基酸残基替换。具有相似侧链的氨基酸残基家族已在本领域定义过。这些家族包括具有碱性侧链的氨基酸(例如，赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如，天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如，甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如，丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如，苏氨酸、缬氨酸、异亮氨酸)和具有芳族侧链的氨基酸(例如，酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此，优选地在SES蛋白中预测的非必需氨基酸残基用来自同一侧链家族的另一氨基酸残基替换。或者，在另一实施方案中，可通过如饱和诱变沿着全部或部分SES编码序列随机导入突变，并根据文中所述的SES活性对所得突变体进行筛选，以鉴定保留SES活性的突变体。对集合A序列之任一诱变后，可重组表达编码蛋白，并用例如文中所述的试验(参见实施例部分的实施例8)确定所述蛋白质的活性。

B.重组表达载体和宿主细胞

本发明的另一方面涉及包含编码SES蛋白(或其部分)的核酸的载体，优选表达载体。如此处所用，术语”载体”是指这样的核酸分子，其可转运与其连接的另一核酸。一种类型的载体为”质粒”，是指其中可连接额外DNA片段的环状双链DNA环。另一种类型的载体为病毒载体，其中可将额外DNA片段连接入病毒基因组。某些载体可在它们所导入的宿主细胞中自主复制(例如，具有细菌复制起点的细菌载体和游离型哺乳动物载体)。其它载体(例如，非游离型哺乳动物载体)在导入宿主细胞后整合入宿主细胞的基因组，并因此随着宿主基因组而复制。而且，某些载体可指导与它们功能性连接的基因的表达。此类载体此处称为”表达载体”。一般，可以用于重组DNA技术中的表达载体为质粒形式。在本说明书中，”质粒”和”载体”可互换使用，因为质粒是最经常使用的载体形式。但是，本发明旨在包括其它形式的表达载体，如病毒载体(例如，复制缺陷型反转录病毒、腺病毒和腺相关病毒)，它们起到类似的作用。

本发明的重组表达载体包含以适于在宿主细胞中表达的形式存在的本发明的核酸，这就是说重组表达载体包括一个或多个调节序列，这些调节序列基于表达所用的宿主细胞进行选择并功能性地与待表达的核酸序列连接。在重组表达载体中，术语“功能性连接”是指目的核苷酸序列与调节序列以允许该核苷酸序列表达(例如，在体外转录/翻译系统中，或当载体导入宿主细胞时在宿主细胞中表达)的方式连接。术语“调节序列”旨在包括启动子、增强子和其它表达调控元件(例如，多聚腺苷酸化信号)。此类调节序列如在Goeddel；基因表达技术：酶学方法(GeneExpression Technology：Methods in Enzymology)185，Academic Press，SanDiego，CA(1990)中描述。调节序列包括控制核苷酸序列在许多类型宿主细胞中组成型表达的调节序列和控制核苷酸序列仅在特定宿主细胞中表达的调节序列。本领域普通技术人员了解，表达载体的设计可取决于如对欲转化宿主细胞的选择、所需的蛋白质表达程度等因素。本发明的表达载体可导入宿主细胞，以产生由此处所述核酸所编码的蛋白质或肽，包括融合蛋白或融合肽(例如，SES蛋白、SES蛋白的突变形式、融合蛋白等)。

本发明的重组表达载体可设计用于在原核或真核细胞中表达SES蛋白。例如，SES基因可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母细胞和其它真菌细胞(参见Romanos，M.A.等人(1992)“在酵母中外源基因的表达：综述”(”Foreign gene Expression in yeast：areview”)，酵母(Yeast)8：423-488；J.W.Bennet & L.L.Lasure编辑的《更多的用于真菌的基因操作》(More Gene Manipulations in Fungi)，Academic Press：San Diego一书中的van den Hondel，C.A.M.J.J.等人(1991)“丝状真菌中的异源基因表达”(”Heterologous gene Expression infilamentous fungi”)，396-428页；以及Peberdy，J.F.等人编辑的《真菌的应用分子遗传学》(Applied Molecular Genetics of Fungi)，CambridgeUniversity Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfersystems and vector development for filamentous fungi)，1-28页)、藻类和单细胞植物细胞(参见Schmidt，R.和Willmitzer，L.(1988)，根癌农杆菌介导的拟南芥叶和子叶外植体的高效转化，植物细胞报道(Plant Cell Rep.)：583-586)、或哺乳动物细胞中表达。合适的宿主细胞在Goeddel，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，CA(1990)中进一步进行了讨论。另外，可体外转录并翻译重组表达载体，如使用T7启动子调节序列和T7聚合酶。

在原核生物中蛋白质的表达主要用含有组成型或诱导型启动子的载体进行，以控制融合或非融合蛋白的表达。融合载体将一些氨基酸添加至其中编码的蛋白质上，通常添加至重组蛋白的氨基末端。此类融合载体一般具有三个任务：1)用于增强重组蛋白的表达；2)用于增加重组蛋白的溶解性；和3)在亲和层析中作为配体而利于重组蛋白的纯化。通常，于融合单元和重组蛋白的连接处导入蛋白酶剪切位点到融合表达载体中，这使得在该融合蛋白纯化后，可将重组蛋白与融合单元分离。这些酶和它们的对应识别序列包括Xa因子、凝血酶和肠激酶。

通常的融合表达载体包括pGEX(Pharmacia Biotech Inc；Smith，D.B.和Johnson，K.S.(1988)基因(Gene)67：31-40)、pMAL(New EnglandBiolabs，Beverly，MA)和pRIT5(Pharmacia，Piscataway，NJ)，它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白A融合至重组靶蛋白。在一个实施方案中，将SES蛋白的编码序列克隆入pGEX表达载体，以产生编码融合蛋白的载体，该蛋白从N-末端至C-末端包含GST-凝血酶切割位点-X蛋白。该融合蛋白可以使用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。通过将该融合蛋白用凝血酶切割可得到不与GST融合的重组SES蛋白。

合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等人(1988)基因(Gene)69：301-315)和pET 11d(Studier等人，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)60-89)。pTrc载体中目标基因的表达依赖于宿主RNA聚合酶从杂合的trp-lac融合启动子的转录。pET 11d载体中目标基因的表达依赖于共表达的病毒RNA聚合酶(T7 gn1)介导的从T7-gn10-lac融合启动子的转录。该病毒聚合酶可以由宿主菌株BL21(DE3)或HMS174(DE3)中居住的、具有位于lacUV 5启动子转录调控下的T7 gn1基因的λ原噬菌体提供。

最大化重组蛋白表达的一个策略是将该蛋白质在对所述重组蛋白进行蛋白酶切割的能力被破坏的宿主细菌中表达(Gottesman，S.，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)119-128)。另一策略是改变欲插入表达载体的核酸的核酸序列，以使编码每一氨基酸的各密码子为选择用于表达的细菌如谷氨酸棒状杆菌所优选使用的密码子(Wada等人(1992)核酸研究(Nucleic Acids Res.)20：2111-2118)。本发明核酸序列的此改变可应用标准的DNA合成技术实施。

在另一实施方案中，SES蛋白表达载体为酵母表达载体。用于在酿酒酵母中表达的载体包括pYepSec1(Baldari，等人(1987)Embo J.6：229-234)，pMFa(Kurjan和Herskowitz，(1982)细胞(Cell)30：933-943)，pJRY88(Schultz等人(1987)基因(Gene)54：113-123)，和pYES2(InvitrogenCorporation，San Diego，CA)。适用于其它真菌如丝状真菌的载体和用于构建载体的方法包括在：J.F.Peberdy等人编辑的《真菌的应用分子遗传学》(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfer systems and vectordevelopment for filamentous fungi)，1-28页中详述的那些。

作为另一可选择方案，本发明的SES蛋白可使用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf 9细胞)中表达蛋白质的杆状病毒载体包括pAc系列(Smith等人(1983)Mol.Cell Biol.3：2156-2165)和pVL系列(Lucklow和Summers(1989)病毒学(Virology)170：31-39)。

在另一实施方案中，本发明的SES蛋白可在单细胞植物细胞(如藻类)或在来自高等植物(如种子植物，诸如作物)的植物细胞中表达。植物表达载体的实例包括在：Becker，D.，KemPer，E.，Schell，J.和Masterson，R.(1992)“具有靠近左边界的选择性标记的新植物双元载体(”New plantbinary vectors with selectable markers located proximal to the leftborder”)，植物分子生物学(Plant Mol.Biol.)20：1195-1197；和Bevan，M.W.(1984)“用于植物转化的双元农杆菌载体”(”Binary Agrobacteriumvectors for plant transformation”)，核酸研究(Nucl.Acid.Res.)12：8711-8721中详述的那些。

又在另一实施方案中，本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed，B.(1987)自然(Nature)329：840)和pMT2PC(Kaufman等人(1987)EMBO J.6：187-195)。当用于哺乳动物细胞时，表达载体的调控功能通常由病毒调控元件提供。例如，常用的启动子来自多瘤病毒、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它合适表达系统参见Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：ALaboratory Manual)，第二版，Cold Spring Harbor Laboratory，ColdSpring Harbor Laboratory Press，Cold Spring Harbor，NY，1989中的第16和17章。

在另一实施方案中，重组哺乳动物表达载体优选地可以指导核酸在特定细胞类型中表达(例如，使用组织-特异性调控元件来表达核酸)。组织-特异性调控元件为本领域公知。合适的组织-特异性启动子的非限制性实例包括白蛋白启动子(肝特异性；Pinkert等人(1987)基因进展(Genes Dev.)1：268-277)、淋巴特异性启动子(Calame and Eaton(1988)Adv.Immunol.43：235-275)，特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8：729-733)和免疫球蛋白启动子(Banerji等人(1983)细胞(Cell)33：729-740；Queen和Baltimore(1983)细胞(Cell)33：741-748)、神经元特异性启动子(例如，神经丝启动子；Byrne和Ruddle(1989)PNAS86：5473-5477)、胰特异性启动子(Edlund等人(1985)科学(Science)230：912-916)、以及乳腺特异性启动子(例如，milk serum启动子；美国专利号4,873,316和欧洲专利申请文件号264,166)。此外，还包括发育调节的启动子，如鼠hox启动子(Kessel和Gruss(1990)科学(Science)249：374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)基因进展(Genes Dev.)3：537-546)。

本发明还提供了含本发明的DNA分子的重组表达载体，其中该DNA分子以反义方向克隆在表达载体中。也就是，DNA分子功能性连接到调控序列上，该连接方式使得可以表达(通过转录该DNA分子)产生与SESmRNA反义的RNA分子。可以对和反义方向克隆的核酸功能性连接的调控序列进行选择以控制反义RNA分子在多种细胞类型中持续表达，例如，可选择病毒启动子和/或增强子或调控序列以控制反义RNA实现组成型组织特异性或细胞类型特异性表达。反义表达载体的形式可为重组质粒、噬菌粒或减毒病毒，其中在高效调控区域的控制下产生反义核酸，而所述调控区域的活性由载体所导入的细胞的类型决定。关于利用反义基因实施基因表达调控的讨论见Weintraub，H.等人，反义RNA作为遗传分析的分子工具，Reviews-Trends in Genetics，Vol.1(1)1986。

本发明的另一方面涉及已导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此处可互换使用。当然，该术语不仅指特定的靶细胞，也指该细胞的后代或潜在后代。由于突变或环境因素细胞在传代过程中可发生某些改变，这样，后代实际上可能并不一定与亲代细胞相同，但其仍包括在此处所用术语的范围之内。

宿主细胞可为原核或真核细胞。例如，SES蛋白可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢(CHO)细胞或COS细胞)中表达。其他合适的宿主细胞为技术人员公知。与谷氨酸棒状杆菌有关的、可以以适宜方式用作本发明核酸和蛋白质分子的宿主细胞的微生物如表3所示。

载体DNA可通过常规转化或转染技术导入原核或真核细胞中。如此处所用，术语“转化”和“转染”，“接合”和“转导”意指本领域内公知的各种将外源核酸(例如DNA)导入宿主细胞的技术，包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖-介导的转染、脂转染、天然感受态、化学介导的转移或电穿孔。转化或转染宿主细胞的合适方法可在Sambrook等人(分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，ColdSpring Harbor Laboratory，Cold Spring Harbor Laboratory Press，ColdSpring Harbor，NY，1989)和其它的实验室手册中找到。

对于哺乳动物细胞的稳定转染，已知根据所用的表达载体和所用的转染技术，仅一小部分的细胞可将外源DNA整合到基因组中。一般将编码选择标记的基因(如抗生素抗性基因)与目的基因一同导入宿主细胞以鉴定和筛选整合体。优选的选择标记包括那些可赋予药物抗性的基因，所述药物如G418、潮霉素和氨甲蝶呤。编码选择标记的核酸可与编码SES蛋白质的基因位于同一载体上被导入宿主细胞或可以位于不同的载体上导入。被导入的核酸稳定转染的细胞可以，例如，通过药物筛选进行鉴定(例如，整合了选择标志基因的细胞将存活，而其他细胞死亡)。

通过制备出含至少部分SES基因的载体——所述部分SES基因中已经导入缺失、添加或替代因而可以改变SES基因(如功能性破坏)——可以生成同源重组微生物。优选地该SES基因为谷氨酸棒状杆菌SES基因，但也可为来自相关细菌或甚至来自哺乳动物、酵母或昆虫的同系物。在一个优选的实施方案中，载体设计为在同源重组后可功能性破坏内源SES基因(即，该基因不再编码功能蛋白质；也称为“敲除”载体)。另外，载体可设计为在同源重组后使内源SES基因发生突变或改变但仍编码功能蛋白质(例如，可改变上游调控区域由此改变内源SES蛋白的表达)。在此同源重组载体中，改变的SES基因部分在其5′和3′端侧翼为SES基因的其它核酸，从而使得载体所携带的外源SES基因和微生物中的内源SES基因之间可以发生同源重组。此额外的两翼SES核酸的长度应足以使与内源基因的同源重组成功进行。通常，载体内包括几千碱基的侧翼DNA(5′及3′端)(参见例如，Thomas，K.R.，和Capecchi，M.R.(1987)细胞(Cell)51：503中对同源重组载体的描述)。使用本领域公知的方法，将该载体导入微生物(例如，通过电穿孔)，并筛选内源SES基因已与导入的SES基因同源重组的细胞。

在另一实施方案中，产生的重组微生物含有可调控导入的基因的表达的选定系统。例如，将SES基因插入载体中使其处于乳糖操纵子的控制下，可以使SES基因仅在存在IPTG时表达。此类调控系统为本领域熟知。

本发明的宿主细胞如培养的原核或真核宿主细胞可用于产生(即表达)SES蛋白。因此，本发明还提供了使用本发明的宿主细胞来产生SES蛋白的方法。在一个实施方案中，该方法包括在合适的培养基中培养本发明的宿主细胞(其中已导入了编码SES蛋白的重组表达载体，或其基因组中已导入了编码野生型或改变的SES蛋白的基因)，直至产生SES蛋白。在另一实施方案中，该方法还包括从培养基或宿主细胞分离SES蛋白。

C.本发明的用途和方法

文中所述的核酸分子、蛋白质、蛋白质同系物、融合蛋白、引物、载体和宿主细胞可用于下述一种或多种方法中：鉴定谷氨酸棒状杆菌及相关生物体；绘制与谷氨酸棒状杆菌相关的生物体的基因组图谱；鉴定并定位目标谷氨酸棒状杆菌序列；进化研究；确定SES蛋白中功能所需的蛋白区域；调节SES蛋白活性；调节一种或多种细胞膜组分的代谢；调节一种或多种化合物的跨膜运输以及调节目标化合物如精细化学品的细胞生产。本发明的SES核酸分子具有多种用途。首先，它们可用于鉴定生物体是否为谷氨酸棒状杆菌或其近亲。它们也可用于在混合的微生物群体中鉴定是否存在谷氨酸棒状杆菌或其相关菌。本发明提供了许多谷氨酸棒状杆菌基因的核酸序列。通过将从微生物单一群体或混合群体的培养物提取的基因组DNA在严格条件下用包含对谷氨酸棒状杆菌而言独特的谷氨酸棒状杆菌基因区域的探针来探测，可确定该生物体是否存在。虽然谷氨酸棒状杆菌自身为非致病的，但它与致病种如白喉棒状杆菌有亲缘关系。这种生物体的检测具有实质上的临床重要性。

本发明的核酸和蛋白质分子也可用作基因组特定区域的标记。这不仅适用于绘制基因组图谱，也适用于谷氨酸棒状杆菌蛋白质的功能研究。例如，可切割谷氨酸棒状杆菌基因组，并将片段与DNA-结合蛋白孵育来确定与特定的谷氨酸棒状杆菌DNA-结合蛋白结合的基因组区域。另外，那些结合蛋白的片段可用本发明的核酸分子探测，优选地用易于检测的标记物实施探测；此核酸分子与基因组片段的结合可将片段定位于谷氨酸棒状杆菌的基因组图谱中，并且当用不同的酶重复此过程几次时，将有利于快速确定蛋白质结合的核酸序列。而且，本发明的核酸分子可以充分同源于亲缘种的序列，这样这些核酸分子可用作在亲缘菌如乳糖发酵短杆菌(Brevibacterium Lactofermentum)中构建基因组图谱的标记。

本发明的SES核酸分子也适用于进化研究和蛋白质结构研究。多种原核和真核细胞应用本发明分子参与的代谢和运输过程；通过将本发明核酸分子的序列与那些来自其它生物体的编码类似酶的序列比较，可确定所述生物体的进化关系。由此，此比较使得可确定哪些区域保守和哪些区域不保守，这有利于确定那些对酶功能所必需的蛋白质区域。这种确定对蛋白质工程研究有价值，并可指出蛋白质可耐受多大的诱变而不丢失其功能。

对本发明SES核酸分子的操作可导致产生与野生型SES蛋白功能不同的SES蛋白。这些蛋白质可在功效或活性上提高，可以在细胞内以比通常多的量存在，或可以在功效或活性上降低。

这种对参与谷氨酸棒状杆菌中DNA修复、重组或者转座的蛋白质的活性的调节将影响细胞的遗传稳定性。例如，可以通过减少参与DNA修复机制的蛋白质的数量或活性来降低细胞纠正遗传错误的能力，这将使得目标突变(如编码参与精细化学品生产的蛋白的那些突变)能更为容易地被导入基因组中。转座子活性或数量的增加将同样导致基因组中突变速率的增加并且可以简单地使目的基因(例如编码参与精细化学品生产的蛋白的目的基因)加倍或者破坏不想要的基因(例如编码降解精细化学品的蛋白的基因)。相反，降低转座子的数量或活性或者增加DNA修复蛋白的数量或活性可以增加谷氨酸棒状杆菌的遗传稳定性，这反过来将导致微生物能在几次培养传代后更好地保持已导入这些微生物的突变。理想的是，在诱变和菌株构建过程中，降低一个或多个DNA修复系统的活性并且增加一个或多个转座子的活性，但是如果在菌株中已经获得目标突变则可以实施相反的事情。通过将一个或多个DNA修复基因或转座子置于可诱导的阻遏物的控制下，该操作是可能的。

对参与谷氨酸棒状杆菌中转录和翻译的蛋白质的调节可以直接和间接影响这些微生物的精细化学品的生产。例如，对直接翻译基因的蛋白(例如聚合酶)或者直接调节转录的蛋白(阻遏物或者激活蛋白)的操作可以直接影响靶基因的表达。对于编码参与精细化学品的生物合成或降解的蛋白质的基因，这种类型的遗传操作将对所述精细化学品的生产具有直接的影响。突变阻遏蛋白使得其不再能够抑制其靶基因或者突变激活蛋白从而优化其活性将导致靶基因的转录增加。如果靶基因是例如精细化学品生物合成基因，结果可能是由于可得到的该基因的转录物的总体量增大，该化学品的生产增加，而且这同样将增加蛋白质的量。靶序列的阻遏蛋白的量或活性的增加或者靶序列的激活蛋白的量或活性的降低在所述序列是例如降解精细化学品的蛋白时将导致所述精细化学品的生产的类似的增加。

对参与转录和翻译的蛋白质的操作也可以间接影响精细化学品的生产。对应答环境或代谢因素而整体调节谷氨酸棒状杆菌中转录的转录因子(例如σ因子)或翻译阻遏物/活化物的活性或数量的调节应可以使细胞转录与环境或代谢调节解偶联。而这将使转录能够在通常应该减慢或停止基因表达的条件，如大规模发酵培养中存在的不利条件(例如高温、低氧含量、高水平废产物)下继续。在这些条件下增加基因(例如精细化学品生物合成基因)的表达速率也可以至少由于细胞中存在相对较大数量的精细化学品生物合成蛋白而增加精细化学品生产的总速率。修饰转录和翻译调节的原理和例子在例如Lewin，B.(1990)基因(Gene)IV，第3部分：“通过转录控制原核生物基因”，牛津大学出版社：牛津，213-301页中描述。

对参与多肽折叠的蛋白质(例如伴侣蛋白)的活性和数量的调节使得可以总体上增加细胞中正确折叠的分子的生产。这具有两种效果：首先，由于更少的蛋白被错误折叠和降解，细胞中蛋白的数量总体增加，其次，正确折叠从而具有活性的给定蛋白的量增加(见例如，Thomas，J.G.，Baneyx，F.(1997)蛋白质表达和纯化11(3)：289-296；Luo，Z.H.，和Hua，Z.C.(1998)国际生物化学和分子生物学46(3)：471-477；Dale，G.E.，等(1994)蛋白质工程7(7)：925-931；Amrein，K.E.等(1995)Proc.Natl.Acad.Sci.U.S.A.92(4)：1048-1052和Caspers，P.，等(1994)Cell.Mol.Biol.40(5)：635-644)。虽然这些突变可以增加任何种类的活性蛋白质的数目，但是当它们与其他能增加例如精细化学品生物合成蛋白的活性或量的突变偶联时，它们可以对正确折叠的活性目标蛋白的量产生加性效应。

操作参与谷氨酸棒状杆菌中多肽分泌的蛋白质从而增加它们的活性或数目能够直接提高该微生物的蛋白质性精细化学品(例如酶)的分泌。收获和纯化被分泌到大规模培养物的培养基中的精细化学品比收获和纯化保留在细胞中的精细化学品要容易得多，因此对该分泌系统的修饰应可以导致精细化学品的产率和产量的增加。对这些分泌蛋白的遗传操作也可以导致一种或多种精细化学品的产量的直接提高。首先，增加或降低一种或多种谷氨酸棒状杆菌分泌系统的活性(通过突变一种或多种参与这些途径的SES蛋白实现)可以导致细胞的分泌速率总体上增加或降低。这些分泌蛋白中的许多(例如，细胞表面蛋白酶或细胞表面受体)具有对细胞存活重要的功能。改变分泌途径使这些蛋白更容易地被运输到它们的细胞外位置将可以增加细胞的总体存活力从而导致在大规模培养中有更多数量的能够产生精细化学品的谷氨酸棒状杆菌细胞。其次，公知特定细菌分泌途径(例如sec系统)也在膜整合蛋白(例如通道、孔或转运蛋白)插入到细胞膜的过程中起着重要的作用。如果一种或多种分泌途径蛋白的活性增加了，由于细胞内营养物的量增加或者细胞内废物的量减少，该细胞生产精细化学品的能力也同样会增加，如果一种或多种此类分泌途径蛋白的活性降低，则可能会没有足够可用于支持目标化合物过量生产的营养物或者可能废产物会干扰该生物合成。

这些上面提到的突变SES蛋白以增加谷氨酸棒状杆菌中精细化学品的产率的策略并不旨在限制；这些突变策略的变通方案对技术人员是相当明显的。使用这些策略(包括本文公开的机制)使得可能使用本发明的核酸和蛋白质分子产生表达突变的SES核酸和蛋白质分子的谷氨酸棒状杆菌或者相关细菌菌株，从而提高目标化合物的产率、产量和/或生产效率。目标化合物可以是用谷氨酸棒状杆菌制备的任何产物，包括生物合成途径的终产物和天然代谢途径的中间产物以及非天然存在于谷氨酸棒状杆菌代谢中的但是被本发明谷氨酸棒状杆菌菌株生产的分子。

下面的实施例对本发明作进一步阐述，但这些实施例不应理解为限制性的。特此将本专利申请中引用的所有参考文献、专利申请、专利、公开的专利申请的全部内容并入作为参考。

实施例

实施例1：谷氨酸棒状杆菌ATCC 13032的总基因组DNA的制备

谷氨酸棒状杆菌(ATCC 13032)的培养物在30℃于BHI培养基(Difco)中剧烈振摇过夜。离心收获细胞，弃上清并将细胞重悬于5ml缓冲液-I(培养物原初体积的5％—所有显示的体积为按100ml培养物体积计算所得)。缓冲液-I的组成：140.34g/l蔗糖，2.46g/l MgSO₄·7H₂O，10ml/l KH₂PO₄溶液(100g/l，用KOH调节至pH 6.7)，50ml/l M12浓缩物(10g/l(NH₄)₂SO₄，1g/lNaCl，2g/l MgSO₄·7H₂O，0.2g/l CaCl₂，0.5g/l酵母提取物(Difco)，10ml/l痕量元素混合物(200mg/l FeSO₄·H₂O，10mg/l ZnSO₄·7H₂O，3mg/lMnCl₂·4H₂O，30mg/l H₃BO₃，20mg/l CoCl₂·6H₂O，1mg/l NiCl₂·6H₂O，3mg/l Na₂MoO₄·2H₂O，500mg/l络合剂(EDTA或柠檬酸)，100ml/l维生素-混合物(0.2mg/l生物素，0.2mg/l叶酸，20mg/l对氨基苯甲酸，20mg/l核黄素，40mg/l泛酸钙，140mg/l烟酸，40mg/l维生素B6盐酸盐，200mg/l肌醇)。向悬液中加入溶菌酶至终浓度2.5mg/ml。在37℃孵育约4小时后，细胞壁降解，离心收获所得的原生质体。沉淀用5ml缓冲液-I洗一次并用5ml TE-缓冲液(10mM Tris-HCl，1mM EDTA，pH 8)洗一次。将沉淀重悬于4mlTE-缓冲液，并加入0.5ml SDS溶液(10％)和0.5ml NaCl溶液(5M)。加入蛋白酶K至终浓度200μg/ml后，将悬液在37℃孵育约18小时。使用标准方法用酚、酚/氯仿/异戊醇和氯仿/异戊醇提取而纯化DNA。然后，加入1/50体积的3M醋酸钠和2体积的乙醇，于-20℃孵育30分钟，并在高速离心机上使用SS34转子(Sorvall)于12,000转/分离心30分钟，沉淀DNA。将该DNA溶于含20μg/ml RNaseA的1ml TE-缓冲液中，并用1000ml TE-缓冲液于4℃透析至少3小时。在此期间，更换缓冲液3次。向分装的0.4ml透析过的DNA等份溶液中加入0.4ml 2M LiCl和0.8ml乙醇。于-20℃孵育30分钟后，离心(13,000转/分，Biofuge Fresco，Heraeus，Hanau，德国)收集DNA。将该DNA沉淀溶于TE-缓冲液。用此方法制备的DNA可用于所有目的，包括southern印迹和构建基因组文库。

实施例2：在大肠杆菌中构建谷氨酸棒状杆菌(ATCC13032)的基因组文库

使用如实施例1所述制备的DNA，按照公知的且已建立的方法(参见例如，Sambrook，J.等人(1989)“分子克隆：实验室手册”，Cold Spring HarborLaboratory Press，或AUSUbel，F.M.等人(1994) “分子生物学最新方法”，John Wiley & Sons.)构建粘粒和质粒文库。

可以使用任何质粒或粘粒。特别优选使用的是质粒pBR322(Sutcliffe，J.G.(1979)美国国家科学院院报(Proc.Natl.Acad.Sci.USA)75：3737-3741)；pACYC177(Change & Cohen(1978)细菌学杂志(J.Bacteriol)134：1141-1156)、pBS系列的质粒(pBSSK+，pBSSK-及其它；Stratagene，LaJolla，USA)、或粘粒如SuperCos1(Stratagene，LaJolla，USA)或Lorist6(Gibson，T.J.，Rosenthal A.和Waterson，R.H.(1987)基因(Gene)53：283-286。

实施例3：DNA序列测定和计算机功能分析

如实施例2所述的基因组文库按照标准方法用于DNA序列测定，尤其是使用ABI377序列测定仪通过链终止法测定(参见例如，Fleischman，R.D.等人(1995)“流感嗜血菌(Haemophilus Influenzae)Rd的全基因组随机序列测定和组装”，科学(Science)，269：496-512)。使用具有以下核苷酸序列的测序引物：5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。

实施例4：体内诱变

谷氨酸棒状杆菌的体内诱变可通过将质粒(或其它载体)DNA在不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如芽孢杆菌属种或酵母如酿酒酵母)中传代而实施。通常的增变菌株在用于DNA修复系统的基因中有突变(例如mutHLS，mutD，mutT等；参考文献见Rupp，W.D.(1996)，《大肠杆菌和沙门氏菌(Salmonella)》中的“DNA修复机制”，ASM：Washington，2277-2294页)。此类菌株为技术人员熟知。此类菌株的用途在例如Greener，A.和Callahan，M.(1994)Strategies 7：32-34中进行了阐述。

实施例5：在大肠杆菌和谷氨酸棒状杆菌之间的DNA转移

几种棒状杆菌和短杆菌物种含有自主复制的内源质粒(例如pHM1519或pBL1)(综述参见例如，Martin，J.F.等人(1987)生物技术(Biotechnology)，5：137-146)。使用标准的大肠杆菌载体可以容易地构建用于大肠杆菌和谷氨酸棒状杆菌的穿梭载体(Sambrook，J.等人(1989)，“分子克隆：实验室手册”，Cold Spring Harbor Laboratory Press，或Ausubel，F.M.等人(1994)“分子生物学最新方法”(”Current Protocols in Molecular Biology”)，JohnWiley & Sons)，其中加入用于谷氨酸棒状杆菌中的复制起点和来自谷氨酸棒状杆菌的适当标记。此类复制起点优选取自从棒状杆菌和短杆菌分离的内源质粒。对于这些物种，作为转化标记尤其有用的是卡那霉素抗性基因(如源自Tn5或Tn903转座子的基因)或氯霉素抗性基因(Winnacker，E.L.(1987)”From Genes to Clones-Introduction to Gene Technology，VCH，Weinheim)。在文献中有许多在大肠杆菌和谷氨酸棒状杆菌中均复制的多种穿梭载体的构建例子，这些载体可用于多种目的，包括基因过表达(参见例如，Yoshihama，M.等人(1985)细菌学杂志(J.Bacteriol.)162：591-597，Martin J.F.等人(1987)生物技术(Biotechnology)，5：137-146和Eikmanns，B.J.等人(1991)基因(Gene)，102：93-98)。

使用标准技术，可将目的基因克隆入上述的穿梭载体之一中并可将此杂合载体导入谷氨酸棒状杆菌菌株。谷氨酸棒状杆菌的转化可通过原生质体转化(Kastsumata，R.等人(1984)细菌学杂志(J.Bacteriol.)159，306-311)、电穿孔(Liebl，E.等人(1989)FEMS Microbiol.Letters，53：399-303)以及当使用特别的载体时，也可通过接合(如在Sch_fer，A等人(1990)细菌学杂志(J.Bacteriol.)172：1663-1666中所述)来完成。也可通过制备谷氨酸棒状杆菌的质粒DNA(使用本领域熟知的标准方法)并将其转化至大肠杆菌，将谷氨酸棒状杆菌的穿梭载体转移至大肠杆菌中。该转化步骤可使用标准方法进行，但有利的是使用Mcr-缺陷的大肠杆菌菌株，如NM522(Gough & Murray(1983)分子生物学杂志(J.Mol.Biol.)166：1-19)。

实施例6：测定突变蛋白质的表达

在转化的宿主细胞中对突变蛋白质活性的观察依赖于突变蛋白质与野生型蛋白质以相似的方式和相似的量表达的事实。确定突变基因的转录水平(指示可用于翻译为基因产物的mRNA量)的适宜方法是进行Northern印迹(参考文献见例如，Ausubel等人(1988)分子生物学最新方法(CurrentProtocols in Molecular Biology)，Wiley：New York)，其中设计为与目的基因结合的引物用可检测标志(通常为放射性的或化学发光的)标记，这样当提取生物体培养物的总RNA、在凝胶上分级分离、转移至稳定的基质上并与该探针孵育后，探针的结合和结合量将指示该基因的mRN的存在和存在量。该信息指示了突变基因的转录程度。通过多种方法可从谷氨酸棒状杆菌中制备总细胞RNA，这些方法均为本领域熟知，如在Bormann，E.R.等人(1992)分子微生物学(Mol.Microbiol.)6：317-326中所描述的。

为了测定从该mRNA翻译的蛋白质的存在或相对量，可使用标准技术如Western印迹(参见例如，Ausubel等人(1988)分子生物学最新方法(Current Protocols in Molecular Biology)，Wiley：New York)。在该方法中，提取细胞总蛋白质、通过凝胶电泳分离、转移至基质如硝化纤维素上并与能和目标蛋白质特异性结合的探针一起孵育，其中所述探针如抗体。该探针通常用易于检测到的化学发光或显色标记物标记。所观察到的标记物的存在和量将指示在细胞中目标突变蛋白质的存在和量。

实施例7：遗传改变的谷氨酸棒状杆菌的生长—培养基和培养条件

遗传修饰的棒状杆菌培养在合成或天然的生长培养基中。用于棒状杆菌的许多种不同生长培养基为人们熟知并易于获得(Lieb等人(1989)Appl.Microbiol.Biotechnol.，32：205-210；von der Osten等人(1998)Biotechnology Letters，11：11-16；Patent DE 4,120,867；在《原核生物》(The Procaryotes)，Volume II，Balows，A.等人编辑.Springer-Verlag一书中的Liebl(1992)“棒状杆菌属”(“The Genus Corynebacteria”))。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源是糖类，如单糖、二糖或多糖。非常好的碳源的实例是葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可通过复杂化合物如糖蜜或其它来自糖加工的副产品向培养基中提供糖。加入不同碳源的混合物也可能是有利的。其它可能的碳源为醇类和有机酸，如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物，或为包含这些化合物的物质。示例性氮源包括氨气和胺盐如NH₄Cl或(NH₄)₂SO₄、NH₄OH、硝酸盐、尿素、氨基酸和复杂氮源如玉米浆、大豆粉、大豆蛋白质、酵母提取物、肉膏等。

可包括在培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、磷盐或硫酸盐。可向培养基中加入螯合物以维持溶液中的金属离子。尤其有用的螯合物包括二羟基酚，如儿茶酚或原儿茶酸盐，和有机酸如柠檬酸。培养基一般也包括其它生长因子如维生素或生长促进剂，其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和维生素B₆。生长因子和盐常来自复杂的培养基组分，如酵母提取物、糖蜜、玉米浆等。培养基的确切组成强烈取决于具体实验并在每一具体情况下单独确定。有关培养基优化的信息在教科书“Applied Microbiol.Physiology，A Practical Approach”(P.M.Rhodes，P.F.Stanbury编辑，IRL Press(1997)53-73页，ISBN 0 19 963577 3)中可找到。也可从供应商处得到生长培养基，如Standard 1(Merck)或BHI(Brain heart infusion，DIFCO)等。

通过加热(1.5巴，121℃加热20分钟)或过滤除菌对所有的培养基组成成份进行灭菌。组成成份可一起灭菌，或需要的话分开灭菌。所有的培养基组成成份可在培养开始时存在，或它们可按需要连续加入或分批加入。

对每一实验分别确定培养条件。温度应在15℃和45℃的范围之间。在实验中温度可保持恒定或可改变。培养基的pH应在5至8.5的范围之间，优选地在7.0附近，可通过向培养基中添加缓冲液而维持。用于此目的的示例性缓冲液为磷酸钾缓冲液。可备选地或同时地使用合成的缓冲液如MOPS、HEPES、ACES和其它。在培养过程中通过加入NaOH或NH₄OH也可维持恒定的培养pH。如果使用复杂的培养基组分如酵母提取物，可减轻对额外的缓冲液的需要，因为许多复杂化合物具有高缓冲容量。如果使用发酵罐培养微生物，用气态氨也可调节pH。

培养时间通常在几小时到几天之间。选择培养时间以允许肉汤中累积最大量的产物。可在多种容器如不同规格的微滴定板、玻璃管、玻璃烧瓶或玻璃或金属发酵罐中实施公开的生长实验。对于筛选大量的克隆，应在微滴定板、玻璃管或在有或没有挡板的摇瓶中培养微生物。优选地使用100ml摇瓶，其中装有10％(体积)的所需生长培养基。该烧瓶应在旋转振荡器(振幅25mm)上以100-300转/分摇动。可通过维持潮湿环境减少蒸发损失；备选地，对蒸发损失应进行数学上的校正。

如果研究经遗传修饰后的克隆，也应分析未修饰的对照克隆或含有无任何插入的基本质粒的对照克隆。使用已于30℃孵育的在琼脂板上生长的细胞接种培养基至OD₆₀₀ 0.5-1.5，其中所述琼脂板如CM板(10g/l葡萄糖，2.5g/l NaCl，2g/l尿素，10g/l聚胨，5g/l酵母抽取物，5g/l肉膏，22g/l琼脂，用2M NaOH调节至pH 6.8)。培养基的接种通过导入来自CM板的谷氨酸棒状杆菌细胞的盐水溶液或添加该细菌的液体预培养物而完成。

实施例8：突变蛋白质功能的体外分析

本领域已建立了对酶活性和动力学参数的确定方法。为了确定特定的修饰的酶的活性，实验应加以修改以适应野生型酶的比活性，这在本领域普通技术人员的能力范围内。例如可在下列参考文献中找到对酶的一般性总结以及有关结构、动力学、原理、方法、应用和多种酶活性的测定例子的具体细节：Dixon，M.和Webb，E.C.，(1979)酶(Enzymes).Longmans：伦敦；Fersht，(1985)酶结构和机制(Enzyme Structure and Mechanism).Freeman：纽约；Walsh，(1979)酶促反应机制(Enzymatic ReactionMechanisms).Freeman：San Francisco；Price，N.C.，Stevens，L.(1982)酶学基础(Fundamentals of Enzymology).Oxford Univ.Press：Oxford；Boyer，P.D.编辑(1983)酶(The Enzymes)，第三版，Academic Press：纽约；Bisswanger，H.，(1994)酶动力学(Enzymkinetik)，第二版.VCH：Weinheim(ISBN 3527300325)；Bergmeyer，H.U.，Bergmeyer，J.，Graβl，M.编辑(1983-1986)酶分析方法(Methods of Enzymatic Analysis)，第三版，I-XII卷，Verlag Chemie：Weinheim；以及Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)(1987)A9卷，”酶”.VCH：Weinheim，352-363页。

可通过许多已建立的方法测定结合DNA的蛋白质的活性，如DNA条带移位分析(也称为凝胶阻滞试验)。此类蛋白质对其它分子表达的影响可使用报告基因试验测定(如在Kolmar，H.等人(1995) EMBO J.14：3895-3904及其中所引用的参考文献中所述)。报告基因测试系统为人们所熟知，并被建立以应用于原核和真核细胞，其中使用酶如β-半乳糖苷酶、绿色荧光蛋白和几种其它的酶。

可根据如在《生物膜，分子结构和功能》(Biomembranes，MolecularStructure and Function)，Springer：Heidelberg一书中的Gennis，R.B.(1989)”孔、通道和转运蛋白”，85-137；199-234和270-322页中所述的技术进行膜转运蛋白活性的测定。

实施例9：分析突变蛋白质对目标产物产生的影响

谷氨酸棒状杆菌中的遗传修饰对目标化合物(如氨基酸)产生的影响可通过如下方式评估：将经修饰的微生物在合适的条件(如上文中所描述的那些条件)下生长，并针对目标产物(即氨基酸)产生的增加来分析培养基和/或细胞成分。此类分析技术为本领域普通技术人员熟知，包括光谱学、薄层色谱法、多种显色法、酶学和微生物学方法、以及分析色谱法如高效液相色谱法(参见例如，Ullman工业化学百科全书(Ullman，Encyclopediaof Industrial Chemistry)，A2卷，89-90和443-613页，VCH：Weinheim(1985)；Fallon，A.等人(1987)《生物化学和分子生物学实验室技术》(LaboratoryTechniques in Biochemistry and Molecular Biology)中的“HPLC在生物化学中的应用”，17卷；Rehm等人(1993)生物技术(Biotechnology)，3卷，III章“产物回收和纯化”，469-714页，VCH：Weinheim；Belter，P.A.等人(1988)生物分离：生物技术中的下游加工(Bioseparations：downstreamprocessing for Biotechnotogy)，John Wiley and Sons；Kennedy，J.F.和Cabral，J.M.S.(1992)生物物质的回收方法(Recovery processes forbiological materials)，John Wiley and Sons；在《Ulmann工业化学百科全书》一书中的Shaeiwitz，J.A.和Henry，J.D.(1988)生物化学分离，B3卷，11章，1-27页，VCH：Weinheim；以及Dechow，F.J.(1989)生物技术中的分离和纯化技术(Separation and purification techniques in biotechnology)，Noyes Publications.)。

除了测定发酵终产物外，还可分析用于产生目标化合物的代谢途径的其它成分如中间体和副产物来确定化合物的总体生产效率。分析方法包括测定培养基中的营养素水平(例如，糖类、碳氢化合物、氮源、磷酸盐和其它离子)、测定生物量组成和生长、分析生物合成途径中共同代谢物的产生及测定发酵过程中产生的气体。这些测定的标准方法在《应用微生物生理学，实用方法》(Applied Microbial Physiology，A Practical Approach)，P.M.Rhodes和P.F.Stanbury编辑，IRL Press，103-129；131-163和165-192页(ISBN：0199635773)及其引用的参考文献中描述。

实施例10：从谷氨酸棒状杆菌培养物中纯化目标产物

可通过多种本领域熟知的方法从谷氨酸棒状杆菌细胞或上述培养物上清回收目标产物。如果目标产物不从细胞向外分泌，可低速离心从培养物中收获细胞、用标准技术如机械力或超声裂解细胞。离心去除细胞碎片，保留含有可溶蛋白质的上清级分用于进一步纯化目标化合物。如果产物从谷氨酸棒状杆菌细胞分泌，则低速离心从培养物中去除细胞，并保留上清级分用于进一步纯化。

将来自任一种纯化方法的上清级分用合适的树脂进行层析，其中目标分子保留在层析树脂上而样本中的许多杂质不保留在层析树脂上，或者杂质保留在层析树脂上而样本不保留。此层析步骤必需时可使用相同或不同的层析树脂重复。本领域普通技术人员在选择合适的层析树脂以及将它们最有效地用于特定的待纯化分子方面是很精通的。可通过过滤或超滤浓缩纯化产物，并贮存在产物稳定性最大的温度下。

有大量的纯化方法为本领域公知，上述纯化方法为非限制性的。此类纯化技术例如在Bailey，J.E.和Ollis，D.F.生物化学工程基础(BiochemicalEngineering Fundamentals)，McGraw-Hill：纽约(1986)中描述。

分离的化合物的身份和纯度可通过本领域的现有技术评定。这些技术包括高效液相色谱(HPLC)、光谱法、显色法、薄层色谱法、NIRS、酶学试验或微生物学方法。此类分析方法编纂于：Patek等人(1994)Appl.Environ.Microbiol.60：133-140；Malakhova等人(1996)生物技术(Biotekhnologiya)11：27-32；和Schmidt等人(1998)生物加工工程(Bioprocess Engineer)19：67-70；Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)(1996)A27卷，VCH：Weinheim，89-90页，521-540页，540-547页，559-566页，575-581页和581-587页；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wileyand Sons；《生物化学和分子生物学中的实验室技术》17卷中的Fallon，A.等人(1987)HPLC在生物化学中的应用。

等价方案

技术人员知道，或可以通过使用简单常规方法确定本发明具体实施方案的大量等价方案。此类等价方案旨在包括在下面的权利要求书中。

表1中信息的理解如下：

在第1列“DNA ID”中，每种情况下相关数字指所附序列表的SEQID NO。所以，“DNA ID”列中的“5”指SEQ ID NO：5。

在第2列“AA ID”中，每种情况下相关数字指所附序列表的SEQ IDNO。因此，“AA ID”列中的“6”指SEQ ID NO：6。

在第3列“标识”中，列出了每个序列的明确的内部名称。

在第4列“AA pos”中，每种情况下相关数字指相同行中多肽序列“AAID”的氨基酸位置。所以，“AA pos”列中“26”是相应所示的多肽序列的氨基酸位置26。位置计算从N末端为+1开始。

在第5列“AA野生型”中，每种情况下相关字母指相应野生型菌株中位于第4列中所示的位置处的氨基酸(用单字母码表示)。

在第6列“AA突变体”中，每种情况下相关字母指相应突变菌株中位于第4列中所示的位置处的氨基酸(用单字母码表示)。

在第7列“功能”中，列出了相应多肽序列的生理学功能。

蛋白原性氨基酸的单字母码：

A丙氨酸

C半胱氨酸

D天冬氨酸

E谷氨酸

F苯丙氨酸

G甘氨酸

H组氨酸

I异亮氨酸

K赖氨酸

L亮氨酸

M甲硫氨酸

N天冬酰胺

P脯氨酸

Q谷氨酰胺

R精氨酸

S丝氨酸

T苏氨酸

V缬氨酸

W色氨酸

Y酪氨酸

表1：

编码与遗传稳定性、基因表达和折叠相关的蛋白质的基因

DNA ID：AA ID：标识 AA pos： AA野生型 AA突变体功能：

1 2 RXA00019 337 P S 单链DNA特异性外切核酸酶RECJ(EC 3.1.-.-)

405 T I 单链DNA特异性外切核酸酶RECJ(EC 3.1.-.-)

504 P S 单链DNA特异性外切核酸酶RECJ(EC 3.1.-.-)

3 4 RXA00061 754 S N DNA聚合酶I(EC 2.7.7.7)

5 6 RXA00209 414 V A 谷氨酰-TRNA(GLN)酰胺转移酶亚基A(EC 6.3.5.-)

454 L F 谷氨酰-TRNA(GLN)酰胺转移酶亚基A(EC 6.3.5.-)

7 8 RXA00211 44 V I 谷氨酰-TRNA(GLN)酰胺转移酶亚基B(EC 6.3.5.-)

9 10 RXA00314 319 E K 半胱氨酰-TRNA合成酶(EC 6.1.1.16)

11 12 RXA00458 170 L F 谷氨酰-TRNA合成酶(EC 6.1.1.17)

13 14 RXA00493 363 A T 60KD陪伴蛋白GROEL

15 16 RXA00588 23 A V 转录延伸因子GREA

17 18 RXA00669 68 A T tRNA假尿苷合酶A(EC 4.2.1.70)

19 20 RXA01061 686 P S 亮氨酰-tRNA合成酶(EC 6.1.1.4)

21 22 RXA01277 704 G S 脯氨酰内肽酶(EC 3.4.21.26)

23 24 RXA01278 543 T I 蛋白翻译延伸因子G(EF-G)

25 26 RXA01284 164 D N 细菌蛋白翻译延伸因子Tu(EF-TU)

362 S F 细菌蛋白翻译延伸因子Tu(EF-TU)

27 28 RXA01344 5 P L DNA指导的RNA聚合酶β链(EC 2.7.7.6)

429 D V DNA指导的RNA聚合酶β链(EC 2.7.7.6)

29 30 RXA01345 308 A V DNAK蛋白

31 32 RXA01404 108 T I 转录阻遏物

33 34 RXA01431 46 A T 硫氧还蛋白

35 36 RXA01438 182 A T 铁氧化还原蛋白NADP还原酶(EC 1.18.1.2)

37 38 RXA01490 277 A V tRNA假尿苷合成酶B(EC 4.2.1.70)

39 40 RXA01493 32 A V Na+驱动的多种药物流出泵

41 42 RXA01559 400 T A 蛋白质移位酶亚基SECD

43 44 RXA01596 334 R C DNA修复蛋白RECN

493 G D DNA修复蛋白RECN

45 46 RXA01651 7 S F 转座酶

33 L F 转座酶

47 48 RXA01710 69 P S 转录调节剂

49 50 RXA01852 120 P S 组氨酰-tRNA合成酶(EC 6.1.1.21)

51 52 RXA01913 61 L F 蛋白翻译延伸因子Ts(EF-Ts)

53 54 RXA02145 321 P L Menaquinol-细胞色素C还原酶细胞色素B亚基

55 56 RXA02236 87 L F 整合宿主因子

57 58 RXA02267 65 A T DNA(胞嘧啶-5)-甲基转移酶(EC 2.1.1.37)

59 60 RXA02280 502 A V 热休克蛋白HTPG

61 62 RXA02388 401 E K CoME操纵子蛋白3

451 V M COME操纵子蛋白3

63 64 RXA02416 484 G D 外切核酸酶ABC亚基A

65 66 RXA02418 45 V I 细菌蛋白翻译起始因子3(IF-3)

67 68 RXA02429 670 M I 蛋白移位酶亚基SECA

69 70 RXA02431 73 A V DNA聚合酶IV

71 72 RXA02445 17 G E 依赖ATP的DNA解旋酶RECG

73 74 RXA02476 167 S F A/G-特异性腺嘌呤糖基化酶(EC 3.2.2.-)

75 76 RXA02726 286 A V 异亮氨酰-tRNA合成酶(EC 6.1.1.5)

77 78 RXA02731 374 E K 外切核酸酶ABC亚基B

398 M L 外切核酸酶ABC亚基B

410 R L 外切核酸酶ABC亚基B

79 80 RXA02736 312 S F 推测的OXPPCYCLE蛋白OPCA

81 82 RXA02742 179 G S DNA/RNA解旋酶(DEAD/DEAH盒家族)

83 84 RXA02748 100 P S 信号识别颗粒，亚基FFH/SRP54

164 G D 信号识别颗粒，亚基FFH/SRP54

85 86 RXA03070 249 A V 转座酶

87 88 RXA03098 164 S N DNA烷基化修复酶

89 90 RXA03206 98 G D D-Tyr-tRNATyr脱酰基酶

91 92 RXA03260 56 S F 转座酶

93 94 RXA03394 11 S F 甲硫氨酰-tRNA合成酶(EC 6.1.1.10)

95 96 RXA03674 342 V I 依赖ATP的解旋酶HEPA

97 98 RXA03793 414 A V RNA聚合酶σ因子RPOD

99 100 RXA06048 3 L F PS1蛋白前体

4 L P PS1蛋白前体

5 T S PS1蛋白前体

9 A T PS1蛋白前体

26 I V PS1蛋白前体

31 S T PS1蛋白前体

66 S N PS1蛋白前体

158 N D PS1蛋白前体

101 102 RXA07005 339 P S 可能的依赖ATP的解旋酶LHR(EC 3.6.1.-)

103 104 RXA07006 239 P L 外切脱氧核糖核酸酶VII大亚基(EC 3.1.11.6)

序列表

<110>巴斯福股份公司(BAsF Aktiengesellschaft)

<120>编码与遗传稳定性、基因表达和折叠相关的蛋白质的基因

<130>O.Z.0050/52974

<160>104

<210>1

<211>1990

<212>DNA

<213>谷氨酸棒状杆菌(Corynebacterium glutamicum)

<220>

<221>CDS

<222>(101)..(1960)

<223>RXA00019

<400>1

accggatacc ttatgaaaca cctggtgagc ggtgtgtttc accccaacaa ccgagtaaaa 60

tatatctagt actattttac gattgaaagt agatttttct atg acc gtt acc tca 115

Met Thr Val Thr Ser

1 5

cca gca gcg ctc gca ctc agc gac atg tcc tat gtg gac atc att aag 163

Pro Ala Ala Leu Ala Leu Ser Asp Met Ser Tyr Val Asp Ile Ile Lys

10 15 20

aag aag cgc gga tgg aca acc gag ttt ttc cac agc acc atc aac acc 211

Lys Lys Arg Gly Trp Thr Thr Glu Phe Phe His Ser Thr Ile Asn Thr

25 30 35

ggt gaa acc acc aca ccg cta cca gac agc gac cgt gcc aca gca cta 259

Gly Glu Thr Thr Thr Pro Leu Pro Asp Ser Asp Arg Ala Thr Ala Leu

40 45 50

atc cat gac cac atc acc aag gct caa gag ata acc atc atc acc gac 307

Ile His Asp His Ile Thr Lys Ala Gln Glu Ile Thr Ile Ile Thr Asp

55 60 65

ttt gat atg gac ggt att tca gcc ggt gtc att gcc tat gca ggt ctt 355

Phe Asp Met Asp Gly Ile Ser Ala Gly Val Ile Ala Tyr Ala Gly Leu

70 75 80 85

gcc gaa ctg ggc gca cag gtc aat at9 gtg gtg ccc gac tat cgt ggc 403

Ala Glu Leu Gly Ala Gln Val Asn Met Val Val Pro Asp Tyr Arg Gly

90 95 100

gaa cga aat gtc aca gcc agc gat att gat cgt gcg cta gag ctc tac 451

Glu Arg Asn Val Thr Ala Ser Asp Ile Asp Arg Ala Leu Glu Leu Tyr

105 110 115

cct gca acc tca ctc atc atc acc tgc gat gtc ggc atc ggc tcc cat 499

Pro Ala Thr Ser Leu Ile Ile Thr Cys Asp Val Gly Ile Gly Ser His

120 125 130

gaa ggt att gcc cgt gct cac gaa cgc agt atc gcc gtc ctg gtc aca 547

Glu Gly Ile Ala Arg Ala His Glu Arg Ser Ile Ala Val Leu Val Thr

135 140 145

gat cac cac atg gag gtc gaa ccc tgc cag gcc gat gtg gtt ctt aac 595

Asp His His Met Glu Val Glu Pro Cys Gln Ala Asp Val Val Leu Asn

150 155 160 165

ccc aac aga att gac tct gac tac ccc aac aaa gat att tgc ggt gcg 643

Pro Asn Arg Ile Asp Ser Asp Tyr Pro Asn Lys Asp Ile Cys Gly Ala

170 175 180

cag gtc att ttc gcc aca ttg agt gac tat gca cgt cgt tat cgg gcg 691

Gln Val Ile Phe Ala Thr Leu Ser Asp Tyr Ala Arg Arg Tyr Arg Ala

185 190 195

gac aag att atc gac att aat ttg ttg gct gtt ttc tca ggc att ggt 739

Asp Lys Ile Ile Asp Ile Asn Leu Leu Ala Val Phe Ser Gly Ile Gly

200 205 210

gca ctc gcc gat gtc atg cct ctc acc cgt gac act cga cca aca gtg 787

Ala Leu Ala Asp Val Met Pro Leu Thr Arg Asp Thr Arg Pro Thr Val

215 220 225

aag cag gct att gcg ttg ctt cgg ctt gct atc cca caa gta agt aaa 835

Lys Gln Ala Ile Ala Leu Leu Arg Leu Ala Ile Pro Gln Val Ser Lys

230 235 240 245

aac cgt ttc ggc ggt tgg gat acc tat gct gca cgc tct gtt aat cct 883

Asn Arg Phe Gly Gly Trp Asp Thr Tyr Ala Ala Arg Ser Val Asn Pro

250 255 260

gat acg tcc aca ctc atg cat att gtc aat gcc agc cag cat gat cac 931

Asp Thr Ser Thr Leu Met His Ile Val Asn Ala Ser Gln His Asp His

265 270 275

cgc ttc att gca gcc ttc caa ggc atc tca att ctt ctt ggt gaa ctg 979

Arg Phe Ile Ala Ala Phe Gln Gly Ile Ser Ile Leu Leu Gly Glu Leu

280 285 290

att gcg caa aag aag cta gta aac atc gac aat att tct gag tca ttc 1027

Ile Ala Gln Lys Lys Leu Val Asn Ile Asp Asn Ile Ser Glu Ser Phe

295 300 305

att ggc ttc act ctt ggt ccg atg ttt aac gct act cgt cgt gtt ggt 1075

Ile Gly Phe Thr Leu Gly Pro Met Phe Asn Ala Thr Arg Arg Val Gly

310 315 320 325

ggc gac atg cac gat tca ttt ctc gtg ttt gcg ccc cat gcc gca cta 1123

Gly Asp Met His Asp Ser Phe Leu Val Phe Ala Pro His Ala Ala Leu

330 335 340

gca tca cag ccg tcg atg aat cca aat cga cat gct gcg atc tct cgc 1171

Ala Ser Gln Pro Ser Met Asn Pro Asn Arg His Ala Ala Ile Ser Arg

345 350 355

atc att gat aac aac gaa cgt cgc aaa gag ctc tcc aag tcc tct tat 1219

Ile Ile Asp Asn Asn Glu Arg Arg Lys Glu Leu Ser Lys Ser Ser Tyr

360 365 370

gct gcc gta cac agc tca gat cag ccc tac gcg ccc ttt gtg tgg ctc 1267

Ala Ala Val His Ser Ser Asp Gln Pro Tyr Ala Pro Phe Val Trp Leu

375 380 385

tct gag gca cca agc ggc att ctt ggt ctc att gcc tca cag ctc act 1315

Ser Glu Ala Pro Ser Gly Ile Leu Gly Leu Ile Ala Ser Gln Leu Thr

390 395 400 405

cgt gag tct gac gtg cct gcc att gtc att aat cca gat acc ttg tcc 1363

Arg Glu Ser Asp Val Pro Ala Ile Val Ile Asn Pro Asp Thr Leu Ser

410 415 420

ggt tca gct cgc tca cct gag tgg gca ccg atc atc acc caa gta aac 1411

Gly Ser Ala Arg Ser Pro Glu Trp Ala Pro Ile Ile Thr Gln Val Asn

425 430 435

acc ctc agc gca caa ggt cac ggc ggt att cat gct gca ggc cat gag 1459

Thr Leu Ser Ala Gln Gly His Gly Gly Ile His Ala Ala Gly His Glu

440 445 450

tac gcc tgt ggt atg cgt ttt gat aac cat gat gac att gtg acc ttt 1507

Tyr Ala Cys Gly Met Arg Phe Asp Asn His Asp Asp Ile Val Thr Phe

455 460 465

gtt gca aca ctc gac gca ctc gat aaa aac acg cca cgg gaa gca cag 1555

Val Ala Thr Leu Asp Ala Leu Asp Lys Asn Thr Pro Arg Glu Ala Gln

470 475 480 485

ccg gca gat ctg cat ttg gtt gac att gac cac gcg cgt cct gtg ctt 1603

Pro Ala Asp Leu His Leu Val Asp Ile Asp His Ala Arg Pro Val Leu

490 495 500

gat aac ccc tca ctc acc caa gag ctc agt acg gtc gat gct gca gtg 1651

Asp Asn Pro Ser Leu Thr Gln Glu Leu Ser Thr Val Asp Ala Ala Val

505 510 515

gat gct gca cag ttg ctt gtt ctc att gat cag ctt gat caa ctg cag 1699

Asp Ala Ala Gln Leu Leu Val Leu Ile Asp Gln Leu Asp Gln Leu Gln

520 525 530

cca ttt gga cat ggt ttt acc tat ccg cgc atc gac gtg acg ttc agg 1747

Pro Phe Gly His Gly Phe Thr Tyr Pro Arg Ile Asp Val Thr Phe Arg

535 540 545

ccg gca gaa aca gaa ttc aag gtt atg ggt cag cac cat caa cat ctc 1795

Pro Ala Glu Thr Glu Phe Lys Val Met Gly Gln His His Gln His Leu

550 555 560 565

aag gtg atc act cac tca ggg ttg acc tta ttg tgg tgg aat aag gct 1843

Lys Val Ile Thr His Ser Gly Leu Thr Leu Leu Trp Trp Asn Lys Ala

570 575 580

cag cag ctc gat gag atc gca cag tct gaa tta gtc acc atg tct gtg 1891

Gln Gln Leu Asp Glu Ile Ala Gln Ser Glu Leu Val Thr Met Ser Val

585 590 595

gag ctc gat gtc aat atg ttc cgt ggg ttt att tcc ccg caa ggc att 1939

Glu Leu Asp Val Asn Met Phe Arg Gly Phe Ile Ser Pro Gln Gly Ile

600 605 610

gtc tct gcg tgc aca gtt atc tagcttggtt gcataagcac caaaaacaac 1990

Val Ser Ala Cys Thr Val Ile

615 620

<210>2

<211>620

<212>PRT

<213>谷氨酸棒状杆菌

<400>2

Met Thr Val Thr Ser Pro Ala Ala Leu Ala Leu Ser Asp Met Ser Tyr

1 5 10 15

Val Asp Ile Ile Lys Lys Lys Arg Gly Trp Thr Thr Glu Phe Phe His

20 25 30

Ser Thr Ile Asn Thr Gly Glu Thr Thr Thr Pro Leu Pro Asp Ser Asp

35 40 45

Arg Ala Thr Ala Leu Ile His Asp His Ile Thr Lys Ala Gln Glu Ile

50 55 60

Thr Ile Ile Thr Asp Phe Asp Met Asp Gly Ile Ser Ala Gly Val Ile

65 70 75 80

Ala Tyr Ala Gly Leu Ala Glu Leu Gly Ala Gln Val Asn Met Val Val

85 90 95

Pro Asp Tyr Arg Gly Glu Arg Asn Val Thr Ala Ser Asp Ile Asp Arg

100 105 110

Ala Leu Glu Leu Tyr Pro Ala Thr Ser Leu Ile Ile Thr Cys Asp Val

115 120 125

Gly Ile Gly Ser His Glu Gly Ile Ala Arg Ala His Glu Arg Ser Ile

130 135 140

Ala Val Leu Val Thr Asp His His Met Glu Val Glu Pro Cys Gln Ala

145 150 155 160

Asp Val Val Leu Asn Pro Asn Arg Ile Asp Ser Asp Tyr Pro Asn Lys

165 170 175

Asp Ile Cys Gly Ala Gln Val Ile Phe Ala Thr Leu Ser Asp Tyr Ala

180 185 190

Arg Arg Tyr Arg Ala Asp Lys Ile Ile Asp Ile Asn Leu Leu Ala Val

195 200 205

Phe Ser Gly Ile Gly Ala Leu Ala Asp Val Met Pro Leu Thr Arg Asp

210 215 220

Thr Arg Pro Thr Val Lys Gln Ala Ile Ala Leu Leu Arg Leu Ala Ile

225 230 235 240

Pro Gln Val Ser Lys Asn Arg Phe Gly Gly Trp Asp Thr Tyr Ala Ala

245 250 255

Arg Ser Val Asn Pro Asp Thr Ser Thr Leu Met His Ile Val Asn Ala

260 265 270

Ser Gln His Asp His Arg Phe Ile Ala Ala Phe Gln Gly Ile Ser Ile

275 280 285

Leu Leu Gly Glu Leu Ile Ala Gln Lys Lys Leu Val Asn Ile Asp Asn

290 295 300

Ile Ser Glu Ser Phe Ile Gly Phe Thr Leu Gly Pro Met Phe Asn Ala

305 310 315 320

Thr Arg Arg Val Gly Gly Asp Met His Asp Ser Phe Leu Val Phe Ala

325 330 335

Pro His Ala Ala Leu Ala Ser Gln Pro Ser Met Asn Pro Asn Arg His

340 345 350

Ala Ala Ile Ser Arg Ile Ile Asp Asn Asn Glu Arg Arg Lys Glu Leu

355 360 365

Ser Lys Ser Ser Tyr Ala Ala Val His Ser Ser Asp Gln Pro Tyr Ala

370 375 380

Pro Phe Val Trp Leu Ser Glu Ala Pro Ser Gly Ile Leu Gly Leu Ile

385 390 395 400

Ala Ser Gln Leu Thr Arg Glu Ser Asp Val Pro Ala Ile Val Ile Asn

405 410 415

Pro Asp Thr Leu Ser Gly Ser Ala Arg Ser Pro Glu Trp Ala Pro Ile

420 425 430

Ile Thr Gln Val Asn Thr Leu Ser Ala Gln Gly His Gly Gly Ile His

435 440 445

Ala Ala Gly His Glu Tyr Ala Cys Gly Met Arg Phe Asp Asn His Asp

450 455 460

Asp Ile Val Thr Phe Val Ala Thr Leu Asp Ala Leu Asp Lys Asn Thr

465 470 475 480

Pro Arg Glu Ala Gln Pro Ala Asp Leu His Leu Val Asp Ile Asp His

485 490 495

Ala Arg Pro Val Leu Asp Asn Pro Ser Leu Thr Gln Glu Leu Ser Thr

500 505 510

Val Asp Ala Ala Val Asp Ala Ala Gln Leu Leu Val Leu Ile Asp Gln

515 520 525

Leu Asp Gln Leu Gln Pro Phe Gly His Gly Phe Thr Tyr Pro Arg Ile

530 535 540

Asp Val Thr Phe Arg Pro Ala Glu Thr Glu Phe Lys Val Met Gly Gln

545 550 555 560

His His Gln His Leu Lys Val Ile Thr His Ser Gly Leu Thr Leu Leu

565 570 575

Trp Trp Asn Lys Ala Gln Gln Leu Asp Glu Ile Ala Gln Ser Glu Leu

580 585 590

Val Thr Met Ser Val Glu Leu Asp Val Asn Met Phe Arg Gly Phe Ile

595 600 605

Ser Pro Gln Gly Ile Val Ser Ala Cys Thr Val Ile

610 615 620

<210>3

<211>2845

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2815)

<223>RXA00061

<400>3

aatcaattgc agaactaacc cggttgtttc cgagccagtc tgaatgactg aaagcaatat 60

tagaccatca atgattagga atggaaatta ggggtctggt ttg ggt gaa tgt gtc 115

Leu Gly Glu Cys Val

1 5

gct aat ttt tcc act cgc cta cac tcg gga ggc gtg act gag aag act 163

Ala Asn Phe Ser Thr Arg Leu His Ser Gly Gly Val Thr Glu Lys Thr

10 15 20

gac cag acc tta atg ctt atc gac ggc cac tcg atg gct ttc cgc gca 211

Asp Gln Thr Leu Met Leu Ile Asp Gly His Ser Met Ala Phe Arg Ala

25 30 35

ttc ttt gct ttg ccg gct gag aat ttc tcc acg tcg ggc ggg cag gcc 259

Phe Phe Ala Leu Pro Ala Glu Asn Phe Ser Thr Ser Gly Gly Gln Ala

40 45 50

acc aat gct gtc tat ggc ttt ctc tcg atg ctg tcc acg ttg ttg aag 307

Thr Asn Ala Val Tyr Gly Phe Leu Ser Met Leu Ser Thr Leu Leu Lys

55 60 65

gat gag cag cct act cat gtg gcg gtg gct ttc gat gtg ggg cgt aag 355

Asp Glu Gln Pro Thr His Val Ala Val Ala Phe Asp Val Gly Arg Lys

70 75 80 85

acg ttc cgt acc gat atg ttc ccg gcg tat aag gcg cag cgt gaa gca 403

Thr Phe Arg Thr Asp Met Phe Pro Ala Tyr Lys Ala Gln Arg Glu Ala

90 95 100

acg cca cct gag ttt aag ggc cag gtg gaa atc ctc aag gag gtg ttg 451

Thr Pro Pro Glu Phe Lys Gly Gln Val Glu Ile Leu Lys Glu Val Leu

105 110 115

tcc act ttg gga att acg act att gag aaa atc gat ttt gag gct gat 499

Ser Thr Leu Gly Ile Thr Thr Ile Glu Lys Ile Asp Phe Glu Ala Asp

120 125 130

gat gtg atc gcc acg ttg tct gtg gcg gcg aaa cct tta ggc ttt aag 547

Asp Val Ile Ala Thr Leu Ser Val Ala Ala Lys Pro Leu Gly Phe Lys

135 140 145

acg ctg att gtt acg ggt gac cgt gat tcc ttc cag ttg gtc aat gac 595

Thr Leu Ile Val Thr Gly Asp Arg Asp Ser Phe Gln Leu Val Asn Asp

150 155 160 165

acc acc acg gtg ttg tat ccg atg aag ggc gtg tct gtg ctg cac cgt 643

Thr Thr Thr Val Leu Tyr Pro Met Lys Gly Val Ser Val Leu His Arg

170 175 180

ttc acg ccg gaa gca gtg gag gag aag tat gga ctg aca ccg agg cag 691

Phe Thr Pro Glu Ala Val Glu Glu Lys Tyr Gly Leu Thr Pro Arg Gln

185 190 195

tat ccg gag ttt gca gcg ctg cgt ggt gat cct tcc gat aac ttg cct 739

Tyr Pro Glu Phe Ala Ala Leu Arg Gly Asp Pro Ser Asp Asn Leu Pro

200 205 210

aat att cct ggc gtg ggc gag aag act gct acc aag tgg att gcc cag 787

Asr Ile Pro Gly Val Gly Glu Lys Thr Ala Thr Lys Trp Ile Ala Gln

215 220 225

tat gaa act ttg gat aat ttg ctt gat cac gct gat gag atc aag ggc 835

Tyr Glu Thr Leu Asp Asn Leu Leu Asp His Ala Asp Glu Ile Lys Gly

230 235 240 245

aag gtt ggc gcc agc ctg cgt gag cgc att gag cag gtc cgg atg aac 883

Lys Val Gly Ala Ser Leu Arg Glu Arg Ile Glu Gln Val Arg Met Asn

250 255 260

cgc aag ctc acg gag atg gtg aag gat ctg gag ctg ccg ctt ggt ccg 931

Arg Lys Leu Thr Glu Met Val Lys Asp Leu Glu Leu Pro Leu Gly Pro

265 270 275

gac gat ttt gag atg aag cct gtg cag gtt gcg gag gtt gcg gcg aag 979

Asp Asp Phe Glu Met Lys Pro Val Gln Val Ala Glu Val Ala Ala Lys

280 285 290

ttt gac gat ctg gag ttt ggt acc aat ttg cgt gag cgg gtg ctg gcg 1027

Phe Asp Asp Leu Glu Phe Gly Thr Asn Leu Arg Glu Arg Val Leu Ala

295 300 305

gtg gtg aag gcc gag ggt tcc gct gcc ccc gtg gag gaa gtg gaa gcg 1075

Val Val Lys Ala Glu Gly Ser Ala Ala Pro Val Glu Glu Val Glu Ala

310 315 320 325

gaa cag gtt gtc gtc gat acg caa tct ttg gcg caa tgg ctg cct gct 1123

Glu Gln Val Val Val Asp Thr Gln Ser Leu Ala Gln Trp Leu Pro Ala

330 335 340

agg gct ggc cag gcg ctt gct tta gcg ctg gct gga gtg gct aaa cct 1171

Arg Ala Gly Gln Ala Leu Ala Leu Ala Leu Ala Gly Val Ala Lys Pro

345 350 355

gct gct ggc gac acg tat gcg cta gcg att gcg gat acc aag cgc cat 1219

Ala Ala Gly Asp Thr Tyr Ala Leu Ala Ile Ala Asp Thr Lys Arg His

360 365 370

gcg gtg ttg gtt gat gtg gct gat att tca gcg gag gat gaa aag gcg 1267

Ala Val Leu Val Asp Val Ala Asp Ile Ser Ala Glu Asp Glu Lys Ala

375 380 385

ctg gcc acg tgg ttg gcg tcg gaa gat cca aag atg ctg cac ggc gct 1315

Leu Ala Thr Trp Leu Ala Ser Glu Asp Pro Lys Met Leu His Gly Ala

390 395 400 405

aag gcc gcc tat cat atg ctc gct ggg cgc ggt ttt gag ctg cac ggc 1363

Lys Ala Ala Tyr His Met Leu Ala Gly Arg Gly Phe Glu Leu His Gly

410 415 420

gtg gtg cat gac acg gcg atc gcg gca tac ttg ctg cgt ccg ggc caa 1411

Val Val His Asp Thr Ala Ile Ala Ala Tyr Leu Leu Arg Pro Gly Gln

425 430 435

cgc acc tat gag ctt gcc gac gtc tac cag cgg cat ctt caa cga cag 1459

Arg Thr Tyr Glu Leu Ala Asp Val Tyr Gln Arg His Leu Gln Arg Gln

440 445 450

ttg tct aca aac gac aat ggc ggc cag ctc acg ctg ctc gac gca gct 1507

Leu Ser Thr Asn Asp Asn Gly Gly Gln Leu Thr Leu Leu Asp Ala Ala

455 460 465

gat gac caa tcg ctt gtt gat gat gtc att gca atc ctt gag ctg tct 1555

Asp Asp Gln Ser Leu Val Asp Asp Val Ile Ala Ile Leu Glu Leu Ser

470 475 480 485

gaa gaa ttg acc aaa cag ctt cag gag att caa gct ttt gag ctt tac 1603

Glu Glu Leu Thr Lys Gln Leu Gln Glu Ile Gln Ala Phe Glu Leu Tyr

490 495 500

cat gac ctg gaa att ccg ctg tcg gga att ctg gcg cgc atg gag gcc 1651

His Asp Leu Glu Ile Pro Leu Ser Gly Ile Leu Ala Arg Met Glu Ala

505 510 515

atc ggt atc gct gtt gat gtt gcc act ttg gaa gag cag ttg aag act 1699

Ile Gly Ile Ala Val Asp Val Ala Thr Leu Glu Glu Gln Leu Lys Thr

520 525 530

ttc att ggt cag gtt gct cag gaa gag gaa gca gct cgc gag ctc gct 1747

Phe Ile Gly Gln Val Ala Gln Glu Glu Glu Ala Ala Arg Glu Leu Ala

535 540 545

gag gat cca acc ctg aat ctc tcg agc ccg aag cag ctg caa gtg gtg 1795

Glu Asp Pro Thr Leu Asn Leu Ser Ser Pro Lys Gln Leu Gln Val Val

550 555 560 565

ctt ttt gag acg ttc gga atg ccg aaa acc aag aaa acc aag acc ggc 1843

Leu Phe Glu Thr Phe Gly Met Pro Lys Thr Lys Lys Thr Lys Thr Gly

570 575 580

tac tct acg gct gcc gcg gaa att gaa gcc cta gcg atc aag aat ccg 1891

Tyr Ser Thr Ala Ala Ala Glu Ile Glu Ala Leu Ala Ile Lys Asn Pro

585 590 595

cac cca ttc cta gat cac ctg ttg gca cac cgt cag tac caa aag atg 1939

His Pro Phe Leu Asp His Leu Leu Ala His Arg Gln Tyr Gln Lys Met

600 605 610

aag acc act ctg gaa ggt ctc atc cgt gag gtg gct cct gat ggc cgt 1987

Lys Thr Thr Leu Glu Gly Leu Ile Arg Glu Val Ala Pro Asp Gly Arg

615 620 625

att cac acc acc ttc aac cag acg gtg gcg tct acg gga cgt ttg tca 2035

Ile His Thr Thr Phe Asn Gln Thr Val Ala Ser Thr Gly Arg Leu Ser

630 635 640 645

tcc act gat ccc aac ctg caa aac att cct gtg cgc act gag gct ggc 2083

Ser Thr Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Glu Ala Gly

650 655 660

cga aag att cgt tcg gga ttc gtc gta ggc gag ggg tat gaa acc ttg 2131

Arg Lys Ile Arg Ser Gly Phe Val Val Gly Glu Gly Tyr Glu Thr Leu

665 670 675

ctg act gcc gac tat tcg cag att gaa atg cgc gtg atg gct cac ctt 2179

Leu Thr Ala Asp Tyr Ser Gln Ile Glu Met Arg Val Met Ala His Leu

680 685 690

tcc cag gac cca ggc ttg att gag gcg tac cgc gaa ggc gaa gac ctg 2227

Ser Gln Asp Pro Gly Leu Ile Glu Ala Tyr Arg Glu Gly Glu Asp Leu

695 700 705

cac aat tac gtg ggt tcc aag gtg ttt aat gtg ccc atc gat ggc gtg 2275

His Asn Tyr Val Gly Ser Lys Val Phe Asn Val pro Ile Asp Gly Val

710 715 720 725

acc cct gag ctg cgt cgc cag gtc aag gcc atg tct tac ggt ctg gtg 2323

Thr Pro Glu Leu Arg Arg Gln Val Lys Ala Met Ser Tyr Gly Leu Val

730 735 740

tac ggc ttg tcc gcg ttt ggt ttg tct cag cag ctg agc att cct gct 2371

Tyr Gly Leu Ser Ala Phe Gly Leu Ser Gln Gln Leu Ser Ile Pro Ala

745 750 755

ggc gaa gcg aag cag atc atg gag tcc tac ttc gag cgc ttc ggc gga 2419

Gly Glu Ala Lys Gln Ile Met Glu Ser Tyr Phe Glu Arg Phe Gly Gly

760 765 770

gta cag cgc tac ctc cgg gag atc gtg gag gag gct cga aaa gct ggc 2467

Val Gln Arg Tyr Leu Arg Glu Ile Val Glu Glu Ala Arg Lys Ala Gly

775 780 785

tac acg gaa acg ctg ttt ggg cgt cgt cgc tac ctg ccg gaa ctg acc 2515

Tyr Thr Glu Thr Leu Phe Gly Arg Arg Arg Tyr Leu Pro Glu Leu Thr

790 795 800 805

tcg gat aac cgt gtc gct cgt gaa aac gct gaa cgt gcc gca ctg aac 2563

Ser Asp Asn Arg Val Ala Arg Glu Asn Ala Glu Arg Ala Ala Leu Asn

810 815 820

gcc ccg att cag gga act gcc gca gac atc atc aag gtg gcc atg atc 2611

Ala Pro Ile Gln Gly Thr Ala Ala Asp Ile Ile Lys Val Ala Met Ile

825 830 835

cgg gtg gac cgt tca ctc aag gaa gct gcc gtg aaa tct cgc gtg ctg 2659

Arg Val Asp Arg Ser Leu Lys Glu Ala Ala Val Lys Ser Arg Val Leu

840 845 850

ctt cag gtg cat gat gaa ttg gtc gtg gaa gta gcg gcc ggt gag ttg 2707

Leu Gln Val His Asp Glu Leu Val Val Glu Val Ala Ala Gly Glu Leu

855 860 865

gaa caa gtc cgt gag att ctg gaa cgc gaa atg gat aac gcc atc aag 2755

Glu Gln Val Arg Glu Ile Leu Glu Arg Glu Met Asp Asn Ala Ile Lys

870 875 880 885

ctg tcc gtt cct ttg gaa gtt tca gct ggt gat ggc gtt aac tgg gat 2803

Leu Ser Val Pro Leu Glu Val Ser Ala Gly Asp Gly Val Asn Trp Asp

890 895 900

gct gca gcg cac taagaggtaa ctgccttttc gtcgacgagc 2845

Ala Ala Ala His

905

<210>4

<211>905

<212>PRT

<213>谷氨酸棒状杆菌

<400>4

Leu Gly Glu Cys Val Ala Asn Phe Ser Thr Arg Leu His Ser Gly Gly

1 5 10 15

Val Thr Glu Lys Thr Asp Gln Thr Leu Met Leu Ile Asp Gly His Ser

20 25 30

Met Ala Phe Arg Ala Phe Phe Ala Leu Pro Ala Glu Asn Phe Ser Thr

35 40 45

Ser Gly Gly Gln Ala Thr Asn Ala Val Tyr Gly Phe Leu Ser Met Leu

50 55 60

Ser Thr Leu Leu Lys Asp Glu Gln Pro Thr His Val Ala Val Ala Phe

65 70 75 80

Asp Val Gly Arg Lys Thr Phe Arg Thr Asp Met Phe Pro Ala Tyr Lys

85 90 95

Ala Gln Arg Glu Ala Thr Pro Pro Glu Phe Lys Gly Gln Val Glu Ile

100 105 110

Leu Lys Glu Val Leu Ser Thr Leu Gly Ile Thr Thr Ile Glu Lys Ile

115 120 125

Asp Phe Glu Ala Asp Asp Val Ile Ala Thr Leu Ser Val Ala Ala Lys

130 135 140

Pro Leu Gly phe Lys Thr Leu Ile Val Thr Gly Asp Arg Asp Ser Phe

145 150 155 160

Gln Leu Val Asn Asp Thr Thr Thr Val Leu Tyr Pro Met Lys Gly Val

165 170 175

Ser Val Leu His Arg Phe Thr Pro Glu Ala Val Glu Glu Lys Tyr Gly

180 185 190

Leu Thr Pro Arg Gln Tyr Pro Glu Phe Ala Ala Leu Arg Gly Asp Pro

195 200 205

Ser Asp Asn Leu Pro Asn Ile Pro Gly Val Gly Glu Lys Thr Ala Thr

210 215 220

Lys Trp 1le Ala Gln Tyr Glu Thr Leu Asp Asn Leu Leu Asp His Ala

225 230 235 240

Asp Glu Ile Lys Gly Lys Val Gly Ala Ser Leu Arg Glu Arg Ile Glu

245 250 255

Gln Val Arg Met Asn Arg Lys Leu Thr Glu Met Val Lys Asp Leu Glu

260 265 270

Leu Pro Leu Gly Pro Asp Asp Phe Glu Met Lys Pro Val Gln Val Ala

275 280 285

Glu Val Ala Ala Lys Phe Asp Asp Leu Glu Phe Gly Thr Asn Leu Arg

290 295 300

Glu Arg Val Leu Ala Val Val Lys Ala Glu Gly Ser Ala Ala Pro Val

305 310 315 320

Glu Glu Val Glu Ala Glu Gln Val Val Val Asp Thr Gln Ser Leu Ala

325 330 335

Gln Trp Leu Pro Ala Arg Ala Gly Gln Ala Leu Ala Leu Ala Leu Ala

340 345 350

Gly Val Ala Lys Pro Ala Ala Gly Asp Thr Tyr Ala Leu Ala Ile Ala

355 360 365

Asp Thr Lys Arg His Ala Val Leu Val Asp Val Ala Asp Ile Ser Ala

370 375 380

Glu Asp Glu Lys Ala Leu Ala Thr Trp Leu Ala Ser Glu Asp Pro Lys

385 390 395 400

Met Leu His Gly Ala Lys Ala Ala Tyr His Met Leu Ala Gly Arg Gly

405 410 415

Phe Glu Leu His Gly Val Val His Asp Thr Ala Ile Ala Ala Tyr Leu

420 425 430

Leu Arg Pro Gly Gln Arg Thr Tyr Glu Leu Ala Asp Val Tyr Gln Arg

435 440 445

His Leu Gln Arg Gln Leu Ser Thr Asn Asp Asn Gly Gly Gln Leu Thr

450 455 460

Leu Leu Asp Ala Ala Asp Asp Gln Ser Leu Val Asp Asp Val Ile Ala

465 470 475 480

Ile Leu Glu Leu Ser Glu Glu Leu Thr Lys Gln Leu Gln Glu Ile Gln

485 490 495

Ala Phe Glu Leu Tyr His Asp Leu Glu Ile Pro Leu Ser Gly Ile Leu

500 505 510

Ala Arg Met Glu Ala Ile Gly Ile Ala Val Asp Val Ala Thr Leu Glu

515 520 525

Glu Gln Leu Lys Thr Phe Ile Gly Gln Val Ala Gln Glu Glu Glu Ala

530 535 540

Ala Arg Glu Leu Ala Glu Asp Pro Thr Leu Asn Leu Ser Ser Pro Lys

545 550 555 560

Gln Leu Gln Val Val Leu Phe Glu Thr Phe Gly Met Pro Lys Thr Lys

565 570 575

Lys Thr Lys Thr Gly Tyr Ser Thr Ala Ala Ala Glu Ile Glu Ala Leu

580 585 590

Ala Ile Lys Asn Pro His Pro Phe Leu Asp His Leu Leu Ala His Arg

595 600 605

Gln Tyr Gln Lys Met Lys Thr Thr Leu Glu Gly Leu Ile Arg Glu Val

610 615 620

Ala Pro Asp Gly Arg Ile His Thr Thr Phe Asn Gln Thr Val Ala Ser

625 630 635 640

Thr Gly Arg Leu Ser Ser Thr Asp Pro Asn Leu Gln Asn Ile Pro Val

645 650 655

Arg Thr Glu Ala Gly Arg Lys Ile Arg Ser Gly Phe Val Val Gly Glu

660 665 670

Gly Tyr Glu Thr Leu Leu Thr Ala Asp Tyr Ser Gln Ile Glu Met Arg

675 680 685

Val Met Ala His Leu Ser Gln Asp Pro Gly Leu Ile Glu Ala Tyr Arg

690 695 700

Glu Gly Glu Asp Leu His Asn Tyr Val Gly Ser Lys Val Phe Asn Val

705 710 715 720

Pro Ile Asp Gly Val Thr Pro Glu Leu Arg Arg Gln Val Lys Ala Met

725 730 735

Ser Tyr Gly Leu Val Tyr Gly Leu Ser Ala Phe Gly Leu Ser Gln Gln

740 745 750

Leu Ser Ile Pro Ala Gly Glu Ala Lys Gln Ile Met Glu Ser Tyr Phe

755 760 765

Glu Arg Phe Gly Gly Val Gln Arg Tyr Leu Arg Glu Ile Val Glu Glu

770 775 780

Ala Arg Lys Ala Gly Tyr Thr Glu Thr Leu Phe Gly Arg Arg Arg Tyr

785 790 795 800

Leu Pro Glu Leu Thr Ser Asp Asn Arg Val Ala Arg Glu Asn Ala Glu

805 810 815

Arg Ala Ala Leu Asn Ala Pro Ile Gln Gly Thr Ala Ala Asp Ile Ile

820 825 830

Lys Val Ala Met Ile Arg Val Asp Arg Ser Leu Lys Glu Ala Ala Val

835 840 845

Lys Ser Arg Val Leu Leu Gln Val His Asp Glu Leu Val Val Glu Val

850 855 860

Ala Ala Gly Glu Leu Glu Gln Val Arg Glu Ile Leu Glu Arg Glu Met

865 870 875 880

Asp Asn Ala Ile Lys Leu Ser Val Pro Leu Glu Val Ser Ala Gly Asp

885 890 895

Gly Val Asn Trp Asp Ala Ala Ala His

900 905

<210>5

<211>1621

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1591)

<223>RXA00209

<400>5

acaagaccct cgatgctgcg gctgcgttgg accaagcgcc cgctgtcgag gatggacgtt 60

ttatggttcc gcagattctg ggtgagggcg actaataatt atg acc aac aag tac 115

Met Thr Asn Lys Tyr

1 5

ctg gtt gaa ggc tct gaa aac gag ctg acc aca aag acc gca gca gag 163

Leu Val Glu Gly Ser Glu Asn Glu Leu Thr Thr Lys Thr Ala Ala Glu

10 15 20

ctg gca ggt ctt att cat tcc cgc gag gta act tcc cgc gag gtt act 211

Leu Ala Gly Leu Ile His Ser Arg Glu Val Thr Ser Arg Glu Val Thr

25 30 35

caa gcg cac cta gat cgc att gct gcg gtt gac ggc gat att cat gca 259

Gln Ala His Leu Asp Arg Ile Ala Ala Val Asp Gly Asp Ile His Ala

40 45 50

ttt ctc cac gtt ggc cag gag gag gcc ctg aac gcg gcg gat gac gtc 307

Phe Leu His Val Gly Gln Glu Glu Ala Leu Asn Ala Ala Asp Asp Val

55 60 65

gat aag cgt cta gac gct gga gag gca cct gcc tcg gct ttg gct ggc 355

Asp Lys Arg Leu Asp Ala Gly Glu Ala Pro Ala Ser Ala Leu Ala Gly

70 75 80 85

gtg ccg ctt gcg ctg aag gat gtc ttt acc acc act gat gcg ccg acc 403

Val Pro Leu Ala Leu Lys Asp Val Phe Thr Thr Thr Asp Ala Pro Thr

90 95 100

acg gcg gca tcg aag atg ctt gag ggc tac atg agc cct tat gac gcg 451

Thr Ala Ala Ser Lys Met Leu Glu Gly Tyr Met Ser Pro Tyr Asp Ala

105 110 115

act gtg acc cgc aag atc cgt gag gct ggc atc cca att ttg ggt aag 499

Thr Val Thr Arg Lys Ile Arg Glu Ala Gly Ile Pro Ile Leu Gly Lys

120 125 130

acc aac atg gat gag ttt gcg atg ggt tcc tcc act gag aac tcc gca 547

Thr Asn Met Asp Glu Phe Ala Met Gly Ser Ser Thr Glu Asn Ser Ala

135 140 145

tac ggc cca acc cac aat ccg tgg gat ctg gag cgc acc gca ggt ggt 595

Tyr Gly Pro Thr His Asn Pro Trp Asp Leu Glu Arg Thr Ala Gly Gly

150 155 160 165

tct ggt ggt ggc tct tca gct gct ctt gct gca ggt cag gcg cca ctt 643

Ser Gly Gly Gly Ser Ser Ala Ala Leu Ala Ala Gly Gln Ala Pro Leu

170 175 180

gcg att ggt act gac act ggt gga tcc atc cgt cag cca gct gcg ctg 691

Ala Ile Gly Thr Asp Thr Gly Gly Ser Ile Arg Gln Pro Ala Ala Leu

185 190 195

acc aac act gtc ggt gtg aag cca acc tac gga acc gta tcc cgt tac 739

Thr Asn Thr Val Gly Val Lys Pro Thr Tyr Gly Thr Val Ser Arg Tyr

200 205 210

ggt ctg att gcg tgt gcg tcc tcc ctg gat cag ggt ggc cca acc gct 787

Gly Leu Ile Ala Cys Ala Ser Ser Leu Asp Gln Gly Gly Pro Thr Ala

215 220 225

cgt act gtt ctg gat acc gcg ctt ttg cac gag gtt atc gca ggc cac 835

Arg Thr Val Leu Asp Thr Ala Leu Leu His Glu Val Ile Ala Gly His

230 235 240 245

gac gct ttt gat gcg acc tcc gtg aat cgt ccg gtt gct cct gtt gtg 883

Asp Ala Phe Asp Ala Thr Ser Val Asn Arg Pro Val Ala Pro Val Val

250 255 260

cag gct gcc cgt gaa ggc gcg aac ggt gac ctg aaa ggc gtg aag gtc 931

Gln Ala Ala Arg Glu Gly Ala Asn Gly Asp Leu Lys Gly Val Lys Val

265 270 275

ggt gtg gtc aag cag ttc gac cgc gac ggc tac cag cct ggc gtg ctt 979

Gly Val Val Lys Gln Phe Asp Arg Asp Gly Tyr Gln Pro Gly Val Leu

280 285 290

gag gca ttc cac gct tct gtt gag cag atg cgc tcc cag ggt gcg gaa 1027

Glu Ala Phe His Ala Ser Val Glu Gln Met Arg Ser Gln Gly Ala Glu

295 300 305

atc gtc gag gtt gat tgc cct cac ttt gat gac gct ctt ggc gcg tac 1075

Ile Val Glu Val Asp Cys Pro His Phe Asp Asp Ala Leu Gly Ala Tyr

310 315 320 325

tac ctg att ctt cct tgt gaa gtt tcc tcc aac ctc gcg cgt ttt gac 1123

Tyr Leu Ile Leu Pro Cys Glu Val Ser Ser Asn Leu Ala Arg Phe Asp

330 335 340

ggc atg cgt tac ggt ttg cgc gct ggt gat gac gga act cgt tcc gcc 1171

Gly Met Arg Tyr Gly Leu Arg Ala Gly Asp Asp Gly Thr Arg Ser Ala

345 350 355

gat gag gtc atg gcg tac acc cgt gcg cag gga ttc ggc cct gag gtt 1219

Asp Glu Val Met Ala Tyr Thr Arg Ala Gln Gly Phe Gly pro Glu Val

360 365 370

aag cgc cgt atc atc ctc ggc act tac gcg ttg tct gtt ggt tac tac 1267

Lys Arg Arg Ile Ile Leu Gly Thr Tyr Ala Leu Ser Val Gly Tyr Tyr

375 380 385

gac gcg tac tac ctg cag gct cag cgc gtt cgt acc ctc att gca cag 1315

Asp Ala Tyr Tyr Leu Gln Ala Gln Arg Val Arg Thr Leu Ile Ala Gln

390 395 400 405

gac ttc gcc aag gct tac gag cag gtc gac atc ttg gtg tcc cca acc 1363

Asp Phe Ala Lys Ala Tyr Glu Gln Val Asp Ile Leu Val Ser Pro Thr

410 415 420

act cca acc acc gcg ttc aag ctg ggg gag aag gtc acc gat ccg ctg 1411

Thr Pro Thr Thr Ala Phe Lys Leu Gly Glu Lys Val Thr Asp Pro Leu

425 430 435

gag atg tac aac ttc gac ttg tgc acc ctg cca ctg aac ctg gct ggt 1459

Glu Met Tyr Asn Phe Asp Leu Cys Thr Leu Pro Leu Asn Leu Ala Gly

440 445 450

ctc gcg ggc atg tcc ctg cct tcc ggc ttg gca tca gat act ggt ctg 1507

Leu Ala Gly Met Ser Leu Pro Ser Gly Leu Ala Ser Asp Thr Gly Leu

455 460 465

cct gtt ggt ttg cag ctg atg gct cct gct ttc cag gac gat cgt ctc 1555

Pro Val Gly Leu Gln Leu Met Ala Pro Ala Phe Gln Asp Asp Arg Leu

470 475 480 485

tac cgc gtc ggc gct gct ttt gaa gct gga cgc aag taggttctaa 1601

Tyr Arg Val Gly Ala Ala Phe Glu Ala Gly Arg Lys

490 495

acccttttta agaaattggc 1621

<210>6

<211>497

<212>PRT

<213>谷氨酸棒状杆菌

<400>6

Met Thr Asn Lys Tyr Leu Val Glu Gly Ser Glu Asn Glu Leu Thr Thr

1 5 10 15

Lys Thr Ala Ala Glu Leu Ala Gly Leu Ile His Ser Arg Glu Val Thr

20 25 30

Ser Arg Glu Val Thr Gln Ala His Leu Asp Arg Ile Ala Ala Val Asp

35 40 45

Gly Asp Ile His Ala Phe Leu His Val Gly Gln Glu Glu Ala Leu Asn

50 55 60

Ala Ala Asp Asp Val Asp Lys Arg Leu Asp Ala Gly Glu Ala Pro Ala

65 70 75 80

Ser Ala Leu Ala Gly Val Pro Leu Ala Leu Lys Asp Val Phe Thr Thr

85 90 95

Thr Asp Ala Pro Thr Thr Ala Ala Ser Lys Met Leu Glu Gly Tyr Met

100 105 110

Ser Pro Tyr Asp Ala Thr Val Thr Arg Lys Ile Arg Glu Ala Gly Ile

115 120 125

Pro Ile Leu Gly Lys Thr Asn Met Asp Glu Phe Ala Met Gly Ser Ser

130 135 140

Thr Glu Asn Ser Ala Tyr Gly Pro Thr His Asn Pro Trp Asp Leu Glu

145 150 155 160

Arg Thr Ala Gly Gly Ser Gly Gly Gly Ser Ser Ala Ala Leu Ala Ala

165 170 175

Gly Gln Ala Pro Leu Ala Ile Gly Thr Asp Thr Gly Gly Ser Ile Arg

180 185 190

Gln Pro Ala Ala Leu Thr Asn Thr Val Gly Val Lys Pro Thr Tyr Gly

195 200 205

Thr Val Ser Arg Tyr Gly Leu Ile Ala Cys Ala Ser Ser Leu Asp Gln

210 215 220

Gly Gly Pro Thr Ala Arg Thr Val Leu Asp Thr Ala Leu Leu His Glu

225 230 235 240

Val Ile Ala Gly His Asp Ala Phe Asp Ala Thr Ser Val Asn Arg Pro

245 250 255

Val Ala Pro Val Val Gln Ala Ala Arg Glu Gly Ala Asn Gly Asp Leu

260 265 270

Lys Gly Val Lys Val Gly Val Val Lys Gln Phe Asp Arg Asp Gly Tyr

275 280 285

Gln Pro Gly Val Leu Glu Ala Phe His Ala Ser Val Glu Gln Met Arg

290 295 300

Ser Gln Gly Ala Glu Ile Val Glu Val Asp Cys Pro His Phe Asp Asp

305 310 315 320

Ala Leu Gly Ala Tyr Tyr Leu Ile Leu Pro Cys Glu Val Ser Ser Asn

325 330 335

Leu Ala Arg Phe Asp Gly Met Arg Tyr Gly Leu Arg Ala Gly Asp Asp

340 345 350

Gly Thr Arg Ser Ala Asp Glu Val Met Ala Tyr Thr Arg Ala Gln Gly

355 360 365

Phe Gly Pro Glu Val Lys Arg Arg Ile Ile Leu Gly Thr Tyr Ala Leu

370 375 380

Ser Val Gly Tyr Tyr Asp Ala Tyr Tyr Leu Gln Ala Gln Arg Val Arg

385 390 395 400

Thr Leu Ile Ala Gln Asp Phe Ala Lys Ala Tyr Glu Gln Val Asp Ile

405 410 415

Leu Val Ser Pro Thr Thr Pro Thr Thr Ala Phe Lys Leu Gly Glu Lys

420 425 430

Val Thr Asp Pro Leu Glu Met Tyr Asn Phe Asp Leu Cys Thr Leu Pro

435 440 445

Leu Asn Leu Ala Gly Leu Ala Gly Met Ser Leu Pro Ser Gly Leu Ala

450 455 460

Ser Asp Thr Gly Leu Pro Val Gly Leu Gln Leu Met Ala Pro Ala Phe

465 470 475 480

Gln Asp Asp Arg Leu Tyr Arg Val Gly Ala Ala Phe Glu Ala Gly Arg

485 490 495

Lys

<210>7

<211>793

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(763)

<223>RXA00211

<400>7

tgagccaaaa tcaataaggt gtttttcagc ctgaggtaaa aatacggtgg tactgtcgaa 60

accaatcatc ccctagtttt gaaaagaagg aagcgagcca atg tca ttc ctg atc 115

Met Ser Phe Leu Ile

1 5

cgc gtc ctg ttg tcc gac acc cca ggc agc ctc gcg tta ctc gct gaa 163

Arg Val Leu Leu Ser Asp Thr Pro Gly Ser Leu Ala Leu Leu Ala Glu

10 15 20

gcc ctt ggg att gta gag gcc aat att caa tcc gtg gac gtg gtg gaa 211

Ala Leu Gly Ile Val Glu Ala Asn Ile Gln Ser Val Asp Val Val Glu

25 30 35

cgc ttc ccc aat ggc acg gtc atg gac gat ctg gtg atc tcc atc cct 259

Arg Phe Pro Asn Gly Thr Val Met Asp Asp Leu Val Ile Ser Ile Pro

40 45 50

cgc gat gtc atg gca gac acc atc atc acc gca gct gaa gaa gtc gac 307

Arg Asp Val Met Ala Asp Thr Ile Ile Thr Ala Ala Glu Glu Val Asp

55 60 65

ggc gtg gag att gat tcc atc cgc cca ttc tcc ggg act gtt gac cgc 355

Gly Val Glu Ile Asp Ser Ile Arg Pro Phe Ser Gly Thr Val Asp Arg

70 75 80 85

cgc gga cag atc caa atg ctg gct gct gtt gct cac caa cgc cgc gat 403

Arg Gly Gln Ile Gln Met Leu Ala Ala Val Ala His Gln Arg Arg Asp

90 95 100

atc acc gca gcg atg gaa gaa atg gtc gat gtc atc ccc cgc acc atg 451

Ile Thr Ala Ala Met Glu Glu Met Val Asp Val Ile Pro Arg Thr Met

105 110 115

acc tct ggt tgg gct ttg gtc att gat cta aaa gga ccc atc act cgc 499

Thr Ser Gly Trp Ala Leu Val Ile Asp Leu Lys Gly Pro Ile Thr Arg

120 125 130

atc gct ggt tcc cta gca gcg ccc gaa gat gac ggc acc gtt ccg gag 547

Ile Ala Gly Ser Leu Ala Ala Pro Glu Asp Asp Gly Thr Val Pro Glu

135 140 145

aac atc gtt ctc aaa gaa gct cgc atg ctc aac ccg gaa aac gat ccg 595

Asn Ile Val Leu Lys Glu Ala Arg Met Leu Asn Pro Glu Asn Asp Pro

150 155 160 165

tgg att cca gag tcc tgg aca ctg ctt gat tct tcc ctt gcc atc gct 643

Trp Ile Pro Glu Ser Trp Thr Leu Leu Asp Ser Ser Leu Ala Ile Ala

170 175 180

ccg atc ggc aag cac ggc ctg gct ctg att atc ggt cgc cct ggt ggc 691

Pro Ile Gly Lys His Gly Leu Ala Leu Ile Ile Gly Arg Pro Gly Gly

185 190 195

cct gat ttc ttg gcc agc gaa gtg gag cac tta ggc caa gtc ggt gac 739

Pro Asp Phe Leu Ala Ser Glu Val Glu His Leu Gly Gln Val Gly Asp

200 205 210

att atc gga gca atg ctt caa aaa taatctgagc tgtttaaaaa atgccccaag 793

Ile Ile Gly Ala Met Leu Gln Lys

215 220

<210>8

<211>221

<212>PRT

<213>谷氨酸棒状杆菌

<400>8

Met Ser Phe Leu Ile Arg Val Leu Leu Ser Asp Thr Pro Gly Ser Leu

1 5 10 15

Ala Leu Leu Ala Glu Ala Leu Gly Ile Val Glu Ala Asn Ile Gln Ser

20 25 30

Val Asp Val Val Glu Arg Phe Pro Asn Gly Thr Val Met Asp Asp Leu

35 40 45

Val Ile Ser Ile Pro Arg Asp Val Met Ala Asp Thr Ile Ile Thr Ala

50 55 60

Ala Glu Glu Val Asp Gly Val Glu Ile Asp Ser Ile Arg Pro Phe Ser

65 70 75 80

Gly Thr Val Asp Arg Arg Gly Gln Ile Gln Met Leu Ala Ala Val Ala

85 90 95

His Gln Arg Arg Asp Ile Thr Ala Ala Met Glu Glu Met Val Asp Val

100 105 110

Ile Pro Arg Thr Met Thr Ser Gly Trp Ala Leu Val Ile Asp Leu Lys

115 120 125

Gly Pro Ile Thr Arg Ile Ala Gly Ser Leu Ala Ala Pro Glu Asp Asp

130 135 140

Gly Thr Val Pro Glu Asn Ile Val Leu Lys Glu Ala Arg Met Leu Asn

145 150 155 160

Pro Glu Asn Asp Pro Trp Ile Pro Glu Ser Trp Thr Leu Leu Asp Ser

165 170 175

Ser Leu Ala Ile Ala Pro Ile Gly Lys His Gly Leu Ala Leu Ile Ile

180 185 190

Gly Arg Pro Gly Gly Pro Asp Phe Leu Ala Ser Glu Val Glu His Leu

195 200 205

Gly Gln Val Gly Asp Ile Ile Gly Ala Met Leu Gln Lys

210 215 220

<210>9

<211>1543

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1513)

<223>RXA00314

<400>9

acctgtaaac acttacggtt tgggcgaaat tgaagcggga gccaacctgc tcaacgtcgc 60

aaagaaagaa gcggtgccag caacaccata agttgaaacc ttg agt gtt cgc aca 115

Leu Ser Val Arg Thr

1 5

cag gtt aga cta ggg gac gtg act cta cgc atc ttt gac acc ggt acc 163

Gln Val Arg Leu Gly Asp Val Thr Leu Arg Ile Phe Asp Thr Gly Thr

10 15 20

cgt acg ctt cga gat ttt aaa cct gtt caa cca ggt cat gcc tcg gtg 211

Arg Thr Leu Arg Asp Phe Lys Pro Val Gln Pro Gly His Ala Ser Val

25 30 35

tac ctg tgt ggt gcc acc ccg caa tct tca ccc cac att gga cat gtt 259

Tyr Leu Cys Gly Ala Thr Pro Gln Ser Ser Pro His Ile Gly His Val

40 45 50

cgt tca gca gta gcg ttt gat att ttg cgc cgc tgg ctc atg gct aag 307

Arg Ser Ala Val Ala Phe Asp Ile Leu Arg Arg Trp Leu Met Ala Lys

55 60 65

gga ctt gat gtg gca ttt gtt cgc aat gtc act gat atc gat gac aag 355

Gly Leu Asp Val Ala Phe Val Arg Asn Val Thr Asp Ile Asp Asp Lys

70 75 80 85

att ctc acc aag gca tct gaa aat ggt cgc cct tgg tgg gaa tgg gtg 403

Ile Leu Thr Lys Ala Ser Glu Asn Gly Arg Pro Trp Trp Glu Trp Val

90 95 100

tcc acc tat gaa cgt gaa ttc acc tgg acg tac aac acg ttg ggt gtg 451

Ser Thr Tyr Glu Arg Glu Phe Thr Trp Thr Tyr Asn Thr Leu Gly Val

105 110 115

ctt cct cca tca acg gag cct cgt gca aca ggc cac gtc act cag atg 499

Leu Pro Pro Ser Thr Glu Pro Arg Ala Thr Gly His Val Thr Gln Met

120 125 130

att aag tac atg cag cgc ttg att gat aac ggc ttt gct tac gcc gtt 547

Ile Lys Tyr Met Gln Arg Leu Ile Asp Asn Gly Phe Ala Tyr Ala Val

135 140 145

gat ggc tct gtg tac ttt gat gtc gca gcg tgg tcc aag gct gaa gga 595

Asp Gly Ser Val Tyr Phe Asp Val Ala Ala Trp Ser Lys Ala Glu Gly

150 155 160 165

tct gac tat ggt tct ttg tcc gga aac cgt gtt gaa gat atg gag cag 643

Ser Asp Tyr Gly Ser Leu Ser Gly Agn Arg Val Glu Asp Met Glu Gln

170 175 180

ggc gag ccc gat aac ttt ggt aag cgg ggg cca cag gac ttt gct ctg 691

Gly Glu Pro Asp Asn Phe Gly Lys Arg Gly Pro Gln Asp Phe Ala Leu

185 190 195

tgg aag gct gcc aaa ccg ggt gag ccg tca tgg cca acc cct tgg gga 739

Trp Lys Ala Ala Lys Pro Gly Glu Pro Ser Trp Pro Thr Pro Trp Gly

200 205 210

gac ggc cgg ccg ggt tgg cat ttg gaa tgc tct gcc atg gcc acc tac 787

Asp Gly Arg Pro Gly Trp His Leu Glu Cys Ser Ala Met Ala Thr Tyr

215 220 225

tat ttg ggt gag caa ttt gat att cac tgt ggt ggt ttg gat ctg caa 835

Tyr Leu Gly Glu Gln Phe Asp Ile His cys Gly Gly Leu Asp Leu Gln

230 235 240 245

ttt cca cac cat gaa aat gaa att gcc cag gca cat gcg gct ggc gat 883

Phe Pro His His Glu Asn Glu Ile Ala Gln Ala His Ala Ala Gly Asp

250 255 260

aaa ttt gcc aac tac tgg atg cac aat cac tgg gta aca atg gcc ggc 931

Lys Phe Ala Asn Tyr Trp Met His Asn His Trp Val Thr Met Ala Gly

265 270 275

gag aaa atg tcc aag tct ttg ggc aat gtt ttg gct gtg ccg gaa atg 979

Glu Lys Met Ser Lys Ser Leu Gly Asn Val Leu Ala Val Pro Glu Met

280 285 290

cta aag cag gtt cgt cct gtc gag ctt cgt tat tac ctt ggg tct gcc 1027

Leu Lys Gln Val Arg Pro Val Glu Leu Arg Tyr Tyr Leu Gly Ser Ala

295 300 305

cat tac cgt tcc gtc ctt gag tat tcc gag agc gct ttg agt gaa gct 1075

His Tyr Arg Ser Val Leu Glu Tyr Ser Glu Ser Ala Leu Ser Glu Ala

310 315 320 325

gcg gtg ggt tac cgt cgc att gag tct ttc ctt gag cgt gtg ggg gat 1123

Ala Val Gly Tyr Arg Arg Ile Glu Ser Phe Leu Glu Arg Val Gly Asp

330 335 340

gtt gag gta ggc gag tgg acg cca ggt ttt gaa gtt gcg atg gat gag 1171

Val Glu Val Gly Glu Trp Thr Pro Gly Phe Glu Val Ala Met Asp Glu

345 350 355

gat att gca gtt cct aag gct ttg gct gaa atc cat aac gct gtc cgc 1219

Asp Ile Ala Val Pro Lys Ala Leu Ala Glu Ile His Asn Ala Val Arg

360 365 370

gag ggc aat gct gcc ttg gat aag ggt gat cgt gag gca gcg gag aag 1267

Glu Gly Asn Ala Ala Leu Asp Lys Gly Asp Arg Glu Ala Ala Glu Lys

375 380 385

ctt gct tcc tcg gtt cgt gcg atg act ggc gtt ttg ggc ttc gac ccc 1315

Leu Ala Ser Ser Val Arg Ala Met Thr Gly Val Leu Gly Phe Asp Pro

390 395 400 405

gtt gaa tgg ggt tca gat gca ggc gct gat ggc aag gca gat aag gcg 1363

Val Glu Trp Gly Ser Asp Ala Gly Ala Asp Gly Lys Ala Asp Lys Ala

410 415 420

ctt gat gtg ctg att tct tcg gag ctt gag cgt cgt gca act gct cgt 1411

Leu Asp Val Leu Ile Ser Ser Glu Leu Glu Arg Arg Ala Thr Ala Arg

425 430 435

gct gag aag aat tgg gcg gtt gct gat gag gtt cga gat cgt ctt gcc 1459

Ala Glu Lys Asn Trp Ala Val Ala Asp Glu Val Arg Asp Arg Leu Ala

440 445 450

gat gct ggt att gag gtt gtg gat acc gca gat ggc gct aca tgg aaa 1507

Asp Ala Gly Ile Glu Val Val Asp Thr Ala Asp Gly Ala Thr Trp Lys

455 460 465

ttg cag taattacaga cacttttaag gagataattt 1543

Leu Gln

470

<210>10

<211>471

<212>PRT

<213>谷氨酸棒状杆菌

<400>10

Leu Ser Val Arg Thr Gln Val Arg Leu Gly Asp Val Thr Leu Arg Ile

1 5 10 15

Phe Asp Thr Gly Thr Arg Thr Leu Arg Asp Phe Lys Pro Val Gln Pro

20 25 30

Gly His Ala Ser Val Tyr Leu Cys Gly Ala Thr Pro Gln Ser Ser Pro

35 40 45

His Ile Gly His Val Arg Ser Ala Val Ala Phe Asp Ile Leu Arg Arg

50 55 60

Trp Leu Met Ala Lys Gly Leu Asp Val Ala Phe Val Arg Asn Val Thr

65 70 75 80

Asp Ile Asp Asp Lys Ile Leu Thr Lys Ala Ser Glu Asn Gly Arg Pro

85 90 95

Trp Trp Glu Trp Val Ser Thr Tyr Glu Arg Glu Phe Thr Trp Thr Tyr

100 105 110

Asn Thr Leu Gly Val Leu Pro Pro Ser Thr Glu Pro Arg Ala Thr Gly

115 120 125

His Val Thr Gln Met Ile Lys Tyr Met Gln Arg Leu Ile Asp Asn Gly

130 135 140

Phe Ala Tyr Ala Val Asp Gly Ser Val Tyr Phe Asp Val Ala Ala Trp

145 150 155 160

Ser Lys Ala Glu Gly Ser Asp Tyr Gly Ser Leu Ser Gly Asn Arg Val

165 170 175

Glu Asp Met Glu Gln Gly Glu Pro Asp Asn Phe Gly Lys Arg Gly Pro

180 185 190

Gln Asp Phe Ala Leu Trp Lys Ala Ala Lys Pro Gly Glu Pro Ser Trp

195 200 205

Pro Thr Pro Trp Gly Asp Gly Arg Pro Gly Trp His Leu Glu Cys Ser

210 215 220

Ala Met Ala Thr Tyr Tyr Leu Gly Glu Gln Phe Asp Ile His Cys Gly

225 230 235 240

Gly Leu Asp Leu Gln Phe Pro His His Glu Asn Glu Ile Ala Gln Ala

245 250 255

His Ala Ala Gly Asp Lys Phe Ala Asn Tyr Trp Met His Asn His Trp

260 265 270

Val Thr Met Ala Gly Glu Lys Met Ser Lys Ser Leu Gly Asn Val Leu

275 280 285

Ala Val Pro Glu Met Leu Lys Gln Val Arg Pro Val Glu Leu Arg Tyr

290 295 300

Tyr Leu Gly Ser Ala His Tyr Arg Ser Val Leu Glu Tyr Ser Glu Ser

305 310 315 320

Ala Leu Ser Glu Ala Ala Val Gly Tyr Arg Arg Ile Glu Ser Phe Leu

325 330 335

Glu Arg Val Gly Asp Val Glu Val Gly Glu Trp Thr Pro Gly Phe Glu

340 345 350

Val Ala Met Asp Glu Asp Ile Ala Val Pro Lys Ala Leu Ala Glu Ile

355 360 365

His Asn Ala Val Arg Glu Gly Asn Ala Ala Leu Asp Lys Gly Asp Arg

370 375 380

Glu Ala Ala Glu Lys Leu Ala Ser Ser Val Arg Ala Met Thr Gly Val

385 390 395 400

Leu Gly Phe Asp Pro Val Glu Trp Gly Ser Asp Ala Gly Ala Asp Gly

405 410 415

Lys Ala Asp Lys Ala Leu Asp Val Leu Ile Ser Ser Glu Leu Glu Arg

420 425 430

Arg Ala Thr Ala Arg Ala Glu Lys Asn Trp Ala Val Ala Asp Glu Val

435 440 445

Arg Asp Arg Leu Ala Asp Ala Gly Ile Glu Val Val Asp Thr Ala Asp

450 455 460

Gly Ala Thr Trp Lys Leu Gln

465 470

<210>11

<211>1009

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(979)

<223>RXA00458

<400>11

cacccctgaa aacctcctca actatcccgg agtgatcatc tccaccgttc aggagaaccc 60

atccgaaaca tggcggcaag tgaacatcta atctagaaac atg gca gga cga tac 115

Met Ala Gly Arg Tyr

1 5

gca cca tca cca agc ggc gac ctt cac ttt ggc aac ctc cgc aca gca 163

Ala Pro Ser Pro Ser Gly Asp Leu His Phe Gly Asn Leu Arg Thr Ala

10 15 20

ctg ctg gcc tgg ctg ttc gcg cgc tcc gaa gga aaa aaa ttc ctc atg 211

Leu Leu Ala Trp Leu Phe Ala Arg Ser Glu Gly Lys Lys Phe Leu Met

25 30 35

cgg gtc gaa gac atc gat gaa caa cgc tca tcc aag gaa tcc gcc gaa 259

Arg Val Glu Asp Ile Asp Glu Gln Arg Ser Ser Lys Glu Ser Ala Glu

40 45 50

agc caa ctc gca gac cta tcc gcc ctg ggt ctc gat tgg gat ggc gac 307

Ser Gln Leu Ala Asp Leu Ser Ala Leu Gly Leu Asp Trp Asp Gly Asp

55 60 65

gtc ctc tac caa tcc aca cgc tac gac gcc tac cgc gca gcc ctt gaa 355

Val Leu Tyr Gln Ser Thr Arg Tyr Asp Ala Tyr Arg Ala Ala Leu Glu

70 75 80 85

aaa cta gac acc tac gaa tgt tat tgc tcg cgc cgg gac atc caa gaa 403

Lys Leu Asp Thr Tyr Glu Cys Tyr Cys Ser Arg Arg Asp Ile Gln Glu

90 95 100

gcc tcg cgg gca ccc cat gtg gct ccg gga gtg tat ccg gga acg tgt 451

Ala Ser Arg Ala Pro His Val Ala Pro Gly Val Tyr Pro Gly Thr Cys

105 110 115

agg gga ttg aag gag gag gaa cgc gtc gaa aag cgt gca acc ttg gct 499

Arg Gly Leu Lys Glu Glu Glu Arg Val Glu Lys Arg Ala Thr Leu Ala

120 125 130

gcg caa aac cgg cac ccc gcc atc cgc ctg cgc gcg cag gta acc tcg 547

Ala Gln Asn Arg His Pro Ala Ile Arg Leu Arg Ala Gln Val Thr Ser

135 140 145

ttt gat ttt cac gac cga ctt cgc ggc cca caa act ggc ccc gta gac 595

Phe Asp Phe His Asp Arg Leu Arg Gly Pro Gln Thr Gly Pro Val Asp

150 155 160 165

gat ttc att ctg ctc cgc ggc ggg cag gaa ccc gga tgg gca tac aac 643

Asp Phe Ile Leu Leu Arg Gly Gly Gln Glu Pro Gly Trp Ala Tyr Asn

170 175 180

tta gct gtc gtc gtc gac gat gcc tac caa ggc gtt gac cag gta gtc 691

Leu Ala Val Val Val Asp Asp Ala Tyr Gln Gly Val Asp Gln Val Val

185 190 195

cgc ggc gac gac cta ctc gat tcc gcc gcg cgc caa gcc tac ctc ggc 739

Arg Gly Asp Asp Leu Leu Asp Ser Ala Ala Arg Gln Ala Tyr Leu Gly

200 205 210

tcg ctg ctg ggc acc ccc gcg ccc gaa tac att cac gtg ccg ctc gtg 787

Ser Leu Leu Gly Thr Pro Ala Pro Glu Tyr Ile His Val Pro Leu Val

215 220 225

ctc aac gcc cac ggc cag cgc ctc gcc aaa cgc gac ggg gca gtg acg 835

Leu Asn Ala His Gly Gln Arg Leu Ala Lys Arg Asp Gly Ala Val Thr

230 235 240 245

ctt aaa gaa atg ctt atc gac gcc ccc ctc cac acc att ttc tcc cgc 883

Leu Lys Glu Met Leu Ile Asp Ala Pro Leu His Thr Ile Phe Ser Arg

250 255 260

ctc gca tca tcg ctc ggc tac gaa ggg gta aat tcc gca ccc caa ttg 931

Leu Ala Ser Ser Leu Gly Tyr Glu Gly Val Asn Ser Ala Pro Gln Leu

265 270 275

ttg gaa att ttc gac ccc aca acc ctc agc cgg gag ccg ttt att tac 979

Leu Glu Ile Phe Asp Pro Thr Thr Leu Ser Arg Glu Pro Phe Ile Tyr

280 285 290

tgaggctcag agggaggggt cattccatct 1009

<210>12

<211>293

<212>PRT

<213>谷氨酸棒状杆菌

<400>12

Met Ala Gly Arg Tyr Ala Pro Ser Pro Ser Gly Asp Leu His Phe Gly

1 5 10 15

Asn Leu Arg Thr Ala Leu Leu Ala Trp Leu Phe Ala Arg Ser Glu Gly

20 25 30

Lys Lys Phe Leu Met Arg Val Glu Asp Ile Asp Glu Gln Arg Ser Ser

35 40 45

Lys Glu Ser Ala Glu Ser Gln Leu Ala Asp Leu Ser Ala Leu Gly Leu

50 55 60

Asp Trp Asp Gly Asp Val Leu Tyr Gln Ser Thr Arg Tyr Asp Ala Tyr

65 70 75 80

Arg Ala Ala Leu Glu Lys Leu Asp Thr Tyr Glu Cys Tyr Cys Ser Arg

85 90 95

Arg Asp Ile Gln Glu Ala Ser Arg Ala Pro His Val Ala Pro Gly Val

100 105 110

Tyr Pro Gly Thr Cys Arg Gly Leu Lys Glu Glu Glu Arg Val Glu Lys

115 120 125

Arg Ala Thr Leu Ala Ala Gln Asn Arg His Pro Ala Ile Arg Leu Arg

130 135 140

Ala Gln Val Thr Ser Phe Asp Phe His Asp Arg Leu Arg Gly Pro Gln

145 150 155 160

Thr Gly Pro Val Asp Asp Phe Ile Leu Leu Arg Gly Gly Gln Glu Pro

165 170 175

Gly Trp Ala Tyr Asn Leu Ala Val Val Val Asp Asp Ala Tyr Gln Gly

180 185 190

Val Asp Gln Val Val Arg Gly Asp Asp Leu Leu Asp Ser Ala Ala Arg

195 200 205

Gln Ala Tyr Leu Gly Ser Leu Leu Gly Thr Pro Ala Pro Glu Tyr Ile

210 215 220

His Val Pro Leu Val Leu Asn Ala His Gly Gln Arg Leu Ala Lys Arg

225 230 235 240

Asp Gly Ala Val Thr Leu Lys Glu Met Leu Ile Asp Ala Pro Leu His

245 250 255

Thr Ile Phe Ser Arg Leu Ala Ser Ser Leu Gly Tyr Glu Gly Val Asn

260 265 270

Ser Ala Pro Gln Leu Leu Glu Ile Phe Asp Pro Thr Thr Leu Ser Arg

275 280 285

Glu Pro Phe Ile Tyr

290

<210>13

<211>1744

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1714)

<223>RXA00493

<400>13

cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60

gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115

Met Ala Lys Leu Ile

1 5

gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163

Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu Arg Gly Val Asp Ala

10 15 20

ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211

Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val

25 30 35

gtt ctt gat aag gca ttc ggc gga cct ctg gtc acc aac gac ggt gtc 259

Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val

40 45 50

acc att gcc cgc gac atc gac ctt gag gat cct ttt gag aac ctc ggt 307

Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly

55 60 65

gcg cag ctg gtg aag tcc gtt gct gtt aag acc aac gac atc gct ggt 355

Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr Asn Asp Ile Ala Gly

70 75 80 85

gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403

Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln Ala Leu Ile Ala Glu

90 95 100

ggc ctg cgc aac gtt gct gct ggc gca aac cca atg gag ctc aac aag 451

Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro Met Glu Leu Asn Lys

105 110 115

ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499

Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu Glu Leu Lys Ala Arg

120 125 130

gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547

Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala Asn Val Ala Thr Val

135 140 145

tca tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gca gcg atg gaa 595

Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val Ala Ala Ala Met Glu

150 155 160 165

aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643

Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser Ile

170 175 180

gag act gct ctc gag gtc acc gaa ggt att tct ttc gac aag ggc tac 691

Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser Phe Asp Lys Gly Tyr

185 190 195

ctt tcc cct tat ttc atc aac gac aac gac act cag cag gct gtc ctg 739

Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr Gln Gln Ala Val Leu

200 205 210

gac aac cct gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787

Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys Ile Ser Ser Leu Pro

215 220 225

gac ttc ctc cca ttg ctg gag aag gtt gtg gag tcc aac cgt cct ttg 835

Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu

230 235 240 245

ctg atc atc gca gaa gac gtc gag ggc gag cct ttg cag acc ctg gtt 883

Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val

250 255 260

gtg aac tcc atc cgc aag acc atc aag gtc gtt gca gtg aag tcc cct 931

Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val Ala Val Lys Ser Pro

265 270 275

tac ttc ggt gac cga cgc aag gcg ttc atg gat gac ctg gct att gtc 979

Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp Asp Leu Ala Ile Val

280 285 290

acc aag gca act gtc gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027

Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly Ile Asn Leu Asn Glu

295 300 305

gct ggc gaa gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075

Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg Ile Thr Val Ser Lys

310 315 320 325

gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123

Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser Ala Glu Asp Val Glu

330 335 340

gca cgt cgc ggc cag atc cgt cgc gaa atc gcc aac acc gat tcc acc 1171

Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala Asn Thr Asp Ser Thr

345 350 355

tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219

Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala Lys Leu Ser Gly Gly

360 365 370

att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267

Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu Thr Glu Val Asn Asp

375 380 385

cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315

Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala Ala Arg Ala Ala Ala

390 395 400 405

caa gaa ggc gtt atc gct ggt ggc ggt tcc gct ttg gtt cag atc gct 1363

Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala Leu Val Gln Ile Ala

410 415 420

gag act ctg aag gct tac gcc gaa gag ttc gaa ggc gac cag aag gtc 1411

Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu Gly Asp Gln Lys Val

425 430 435

ggc gtt cgc gca ctg gct act gct ttg ggc aag cca gcg tac tgg atc 1459

Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys Pro Ala Tyr Trp Ile

440 445 450

gcc tcc aac gca ggt ctt gac ggc tct gtt gtt gtt gca cgc act gct 1507

Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val Val Ala Arg Thr Ala

455 460 465

gct ctg cca aac ggc gag ggc ttc aac gct gca act ttg gaa tac gga 1555

Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala Thr Leu Glu Tyr Gly

470 475 480 485

aac ctg atc aac gac ggt gtc atc gac cca gtc aag gtc acc cat tcc 1603

Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val Lys Val Thr His Ser

490 495 500

gca gta gtg aat gca acc tct gtt gca cgc atg gtt ctg acc act gag 1651

Ala Val Val Asn Ala Thr Ser Val Ala Arg Met Val Leu Thr Thr Glu

505 510 515

gct tct gtt gtt gag aag cct gca gaa gaa gca gcc gat gca cat gca 1699

Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala Ala Asp Ala His Ala

520 525 530

gga cat cat cac cac taaagttctg tgaaaaacac cgtggggcag 1744

Gly His His His His

535

<210>14

<211>538

<212>PRT

<213>谷氨酸棒状杆菌

<400>14

Met Ala Lys Leu Ile Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu

1 5 10 15

Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro

20 25 30

Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val

35 40 45

Thr Asn Asp Gly Val Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro

50 55 60

Phe Glu Asn Leu Gly Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr

65 70 75 80

Asn Asp Ile Ala Gly Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln

85 90 95

Ala Leu Ile Ala Glu Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro

100 105 110

Met Glu Leu Asn Lys Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu

115 120 125

Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala

130 135 140

Asn Val Ala Thr Val Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val

145 150 155 160

Ala Ala Ala Met Glu Lys Val Gly Lys Asp Gly Val Val Thr Val Glu

165 170 175

Glu Ser Gln Ser Ile Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser

180 185 190

Phe Asp Lys Gly Iyr Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr

195 200 205

Gln Gln Ala Val Leu Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys

210 215 220

Ile Ser Ser Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu

225 230 235 240

Ser Asn Arg Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro

245 250 255

Leu Gln Thr Leu Val Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val

260 265 270

Ala Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp

275 280 285

Asp Leu Ala Ile Val Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly

290 295 300

Ile Asn Leu Asn Glu Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg

305 310 315 320

Ile Thr Val Ser Lys Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser

325 330 335

Ala Glu Asp Val Glu Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala

340 345 350

Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala

355 360 365

Lys Leu Ser Gly Gly Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu

370 375 380

Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala

385 390 395 400

Ala Arg Ala Ala Ala Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala

405 410 415

Leu Val Gln Ile Ala Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu

420 425 430

Gly Asp Gln Lys Val Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys

435 440 445

Pro Ala Tyr Trp Ile Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val

450 455 460

Val Ala Arg Thr Ala Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala

465 470 475 480

Thr Leu Glu Tyr Gly Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val

485 490 495

Lys Val Thr His Ser Ala Val Val Asn Ala Thr Ser Val Ala Arg Met

500 505 510

Val Leu Thr Thr Glu Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala

515 520 525

Ala Asp Ala His Ala Gly His His His His

530 535

<210>15

<211>652

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(622)

<223>RXA00588

<400>15

tcatacatct tggccccgga aaaccggggc caatcttatg gctcaagtcg ctagttagcc 60

gatgatccac ctctactgtt ccccaggagg gtaagtaatt atg gca agt gta gat 115

Met Ala Ser Val Asp

1 5

aag caa tac atc acc cca gaa acc aag gcc aag ctg gag gaa gag ctc 163

Lys Gln Tyr Ile Thr Pro Glu Thr Lys Ala Lys Leu Glu Glu Glu Leu

10 15 20

aac gcc ctc atc gca cac cgc cct gca gtt gct gcg gaa atc aat gag 211

Asn Ala Leu Ile Ala His Arg Pro Ala Val Ala Ala Glu Ile Asn Glu

25 30 35

cgc cgt gaa gaa ggc gac ctc aag gaa aac gct ggc tat gac gcc gct 259

Arg Arg Glu Glu Gly Asp Leu Lys Glu Asn Ala Gly Tyr Asp Ala Ala

40 45 50

cgt gaa atg cag gac cag gaa gag gcc cgc atc aag cag atc tct gag 307

Arg Glu Met Gln Asp Gln Glu Glu Ala Arg Ile Lys Gln Ile Ser Glu

55 60 65

ctg ctg gcc aac tcc acc act gag cgc gaa ggc atc atc gaa ggt gtc 355

Leu Leu Ala Asn Ser Thr Thr Glu Arg Glu Gly Ile Ile Glu Gly Val

70 75 80 85

gca aac gtt ggc tcc gtt gtt cac gtc tac tac gac ggc gac gag aac 403

Ala Asn Val Gly Ser Val Val His Val Tyr Tyr Asp Gly Asp Glu Asn

90 95 100

gac aag gaa acc ttc ctc atc ggt acc cgt gct ggc gct tcc gag aac 451

Asp Lys Glu Thr Phe Leu Ile Gly Thr Arg Ala Gly Ala Ser Glu Asn

105 110 115

cca gat ctt gag acc tac tct gag cag tcc cca ctc ggc gct gca att 499

Pro Asp Leu Glu Thr Tyr Ser Glu Gln Ser Pro Leu Gly Ala Ala Ile

120 125 130

ctc gga gct cag gaa ggc gac acc cgt cag tac acc gct cca aat ggt 547

Leu Gly Ala Gln Glu Gly Asp Thr Arg Gln Tyr Thr Ala Pro Asn Gly

135 140 145

tcc gtt atc tcc gta act gtt gtt tct gca gaa cca tac aac tca gca 595

Ser Val Ile Ser Val Thr Val Val Ser Ala Glu Pro Tyr Asn Ser Ala

150 155 160 165

aaa gcc gcg aca ctc cgc ggc aaa aac taaccaagga tttaaaagtc 642

Lys Ala Ala Thr Leu Arg Gly Lys Asn

170

ttcaaaatga 652

<210>16

<211>174

<212>PRT

<213>谷氨酸棒状杆菌

<400>16

Met Ala Ser Val Asp Lys Gln Tyr Ile Thr Pro Glu Thr Lys Ala Lys

1 5 10 15

Leu Glu Glu Glu Leu Asn Ala Leu Ile Ala His Arg Pro Ala Val Ala

20 25 30

Ala Glu Ile Asn Glu Arg Arg Glu Glu Gly Asp Leu Lys Glu Asn Ala

35 40 45

Gly Tyr Asp Ala Ala Arg Glu Met Gln Asp Gln Glu Glu Ala Arg Ile

50 55 60

Lys Gln Ile Ser Glu Leu Leu Ala Asn Ser Thr Thr Glu Arg Glu Gly

65 70 75 80

Ile Ile Glu Gly Val Ala Asn Val Gly Ser Val Val His Val Tyr Tyr

85 90 95

Asp Gly Asp Glu Asn Asp Lys Glu Thr Phe Leu Ile Gly Thr Arg Ala

100 105 110

Gly Ala Ser Glu Asn Pro Asp Leu Glu Thr Tyr Ser Glu Gln Ser Pro

115 120 125

Leu Gly Ala Ala Ile Leu Gly Ala Gln Glu Gly Asp Thr Arg Gln Tyr

130 135 140

Thr Ala Pro Asn Gly Ser Val Ile Ser Val Thr Val Val Ser Ala Glu

145 150 155 160

Pro Tyr Asn Ser Ala Lys Ala Ala Thr Leu Arg Gly Lys Asn

165 170

<210>17

<211>1012

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(982)

<223>RXA00669

<400>17

tttactgcgg gcattttacg tatctgcacc ccgcccggct gcgctgagca gccgtaaagc 60

gtggggcgtg acgtcgaaaa gcaaaaaatg aaaggcagac atg gac aat tca acg 115

Met Asp Asn Ser Thr

1 5

gtg cga atc cgg ctg gat cta gcg tat gac ggc acg gat ttt cat ggc 163

Val Arg Ile Arg Leu Asp Leu Ala Tyr Asp Gly Thr Asp Phe His Gly

10 15 20

tgg gcg aag cag ggg acc agc gat cta cgc acc gtg caa aaa gtg ttg 211

Trp Ala Lys Gln Gly Thr Ser Asp Leu Arg Thr Val Gln Lys Val Leu

25 30 35

gaa gac aat ttg agc atg gtg ctg cgt gag act gtt gaa ttg act gtg 259

Glu Asp Asn Leu Ser Met Val Leu Arg Glu Thr Val Glu Leu Thr Val

40 45 50

gcc ggg cga acc gat gcg ggg gtg cat gcg gcg ggc cag gtg gcg cac 307

Ala Gly Arg Thr Asp Ala Gly Val His Ala Ala Gly Gln Val Ala His

55 60 65

ttt gat att ccg gca cac gct tta gag cag cgc agt att gat ggc gat 355

Phe Asp Ile Pro Ala His Ala Leu Glu Gln Arg Ser Ile Asp Gly Asp

70 75 80 85

cca agc aag ttg gtt cgg cgc ttg ggt cgg ttg ctg ccc gat gat att 403

Pro Ser Lys Leu Val Arg Arg Leu Gly Arg Leu Leu Pro Asp Asp Ile

90 95 100

cgg gtg cat ggc gta cgt ttt gcc gag ccc ggg ttt gat gcg cga ttt 451

Arg Val His Gly Val Arg Phe Ala Glu Pro Gly Phe Asp Ala Arg Phe

105 110 115

tcc gcg atg cgc agg cac tac gtt tat cgc att acg acg cat ccc gcc 499

Ser Ala Met Arg Arg His Tyr Val Tyr Arg Ile Thr Thr His Pro Ala

120 125 130

ggc gcg ctg cct acg cgc cgc cac gac acg gcg cag tgg cca aaa cct 547

Gly Ala Leu Pro Thr Arg Arg His Asp Thr Ala Gln Trp Pro Lys Pro

135 140 145

gtc gaa cta gag cgg atg caa tta gcc gcc gat gca ctg ctg ggg ctg 595

Val Glu Leu Glu Arg Met Gln Leu Ala Ala Asp Ala Leu Leu Gly Leu

150 155 160 165

cat gat ttt gtg gcg ttt tgc aaa gct aag cca cat gcg acg acg gtg 643

His Asp Phe Val Ala Phe Cys Lys Ala Lys Pro His Ala Thr Thr Val

170 175 180

cgt gaa cta caa aaa ttt gcg tgg aaa gac gtc tcc act gac atc gaa 691

Arg Glu Leu Gln Lys Phe Ala Trp Lys Asp Val Ser Thr Asp Ile Glu

185 190 195

ccg cag gtg tat gaa gca cac gtg gtg gcc gat gct ttt tgc tgg tcg 739

Pro Gln Val Tyr Glu Ala His Val Val Ala Asp Ala Phe Cys Trp Ser

200 205 210

atg gtg cgc tcg ctg gtc ggc tcc tgc atg gcc gtg ggc gaa gga cgc 787

Met Val Arg Ser Leu Val Gly Ser Cys Met Ala Val Gly Glu Gly Arg

215 220 225

cgc gga tca ggg ttt act gca gaa ttg ctt gat gca agc gaa cgc agc 835

Arg Gly Ser Gly Phe Thr Ala Glu Leu Leu Asp Ala Ser Glu Arg Ser

230 235 240 245

ccc atg gtt cca gta gca cct gcg aaa ggt ttg agc ttg gtt ggc gtg 883

Pro Met Val Pro Val Ala Pro Ala Lys Gly Leu Ser Leu Val Gly Val

250 255 260

gat tat cct tcc gct gat aag tta cag gaa aga gcg ctg gaa acc cga 931

Asp Tyr Pro Ser Ala Asp Lys Leu Gln Glu Arg Ala Leu Glu Thr Arg

265 270 275

gct gtt cgc gag ttt ccg gac gcg tcc gcg agc cta aaa cta gat gat 979

Ala Val Arg Glu Phe Pro Asp Ala Ser Ala Ser Leu Lys Leu Asp Asp

280 285 290

gag taaaagggac taaactcgtc tctcgtatct 1012

Glu

<210>18

<211>294

<212>PRT

<213>谷氨酸棒状杆菌

<400>18

Met Asp Asn Ser Thr Val Arg Ile Arg Leu Asp Leu Ala Tyr Asp Gly

1 5 10 15

Thr Asp Phe His Gly Trp Ala Lys Gln Gly Thr Ser Asp Leu Arg Thr

20 25 30

Val Gln Lys Val Leu Glu Asp Asn Leu Ser Met Val Leu Arg Glu Thr

35 40 45

Val Glu Leu Thr Val Ala Gly Arg Thr Asp Ala Gly Val His Ala Ala

50 55 60

Gly Gln Val Ala His Phe Asp Ile Pro Ala His Ala Leu Glu Gln Arg

65 70 75 80

Ser Ile Asp Gly Asp Pro Ser Lys Leu Val Arg Arg Leu Gly Arg Leu

85 90 95

Leu Pro Asp Asp Ile Arg Val His Gly Val Arg Phe Ala Glu Pro Gly

100 105 110

Phe Asp Ala Arg Phe Ser Ala Met Arg Arg His Tyr Val Tyr Arg Ile

115 120 125

Thr Thr His Pro Ala Gly Ala Leu Pro Thr Arg Arg His Asp Thr Ala

130 135 140

Gln Trp Pro Lys Pro Val Glu Leu Glu Arg Met Gln Leu Ala Ala Asp

145 150 155 160

Ala Leu Leu Gly Leu His Asp Phe Val Ala Phe Cys Lys Ala Lys Pro

165 170 175

His Ala Thr Thr Val Arg Glu Leu Gln Lys Phe Ala Trp Lys Asp Val

180 185 190

Ser Thr Asp Ile Glu Pro Gln Val Tyr Glu Ala His Val Val Ala Asp

195 200 205

Ala Phe Cys Trp Ser Met Val Arg Ser Leu Val Gly Ser Cys Met Ala

210 215 220

Val Gly Glu Gly Arg Arg Gly Ser Gly Phe Thr Ala Glu Leu Leu Asp

225 230 235 240

Ala Ser Glu Arg Ser Pro Met Val Pro Val Ala Pro Ala Lys Gly Leu

245 250 255

Ser Leu Val Gly Val Asp Tyr Pro Ser Ala Asp Lys Leu Gln Glu Arg

260 265 270

Ala Leu Glu Thr Arg Ala Val Arg Glu Phe Pro Asp Ala Ser Ala Ser

275 280 285

Leu Lys Leu Asp Asp Glu

290

<210>19

<211>3022

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2992)

<223>RXA01061

<400>19

gcagacaaga ctgagcagtc cgacggcgat aagcagtggt ttccacataa ttcttcaagt 60

ctatctactt attgagggga ggaagaattg ccctccacac atg aga tgt ccc gtg 115

Met Arg Cys Pro Val

1 5

tac cta cta cac tgt tta acc atg act aac ccg agc gaa ggc acc act 163

Tyr Leu Leu His Cys Leu Thr Met Thr Asn Pro Ser Glu Gly Thr Thr

10 15 20

ccc ctg gcg ttc cgt tat acc ccg gaa ctc gcc aac aag atc gaa ggt 211

Pro Leu Ala Phe Arg Tyr Thr Pro Glu Leu Ala Asn Lys Ile Glu Gly

25 30 35

gag tgg cag aat tac tgg act gac aac ggc aca ttc aac gca ccc aac 259

Glu Trp Gln Asn Tyr Trp Thr Asp Asn Gly Thr Phe Asn Ala Pro Asn

40 45 50

cca gtg ggt gat tta gcg cct gcg gac ggt aaa gca ctt cct gag gac 307

Pro Val Gly Asp Leu Ala Pro Ala Asp Gly Lys Ala Leu Pro Glu Asp

55 60 65

aag ctc ttt gtc cag gat atg ttc ccg tac cca tcc gga gct ggc ctg 355

Lys Leu Phe Val Gln Asp Met Phe Pro Tyr Pro Ser Gly Ala Gly Leu

70 75 80 85

cac gta ggc cac cca ctc ggt tac atc gca acg gat gtt ttc gcc cgc 403

His Val Gly His Pro Leu Gly Tyr Ile Ala Thr Asp Val Phe Ala Arg

90 95 100

tac aac cgc atg ctg ggc aag aac gtt ctg cac acc ttg ggc tat gac 451

Tyr Asn Arg Met Leu Gly Lys Asn Val Leu His Thr Leu Gly Tyr Asp

105 110 115

gcc ttc gga ctg cca gca gag cag tac gcg atc caa acc ggt aca cac 499

Ala Phe Gly Leu Pro Ala Glu Gln Tyr Ala Ile Gln Thr Gly Thr His

120 125 130

cca cgc acc acc acc atg gcc aac att gag aac atg aag cgc cag ctc 547

Pro Arg Thr Thr Thr Met Ala Asn Ile Glu Asn Met Lys Arg Gln Leu

135 140 145

ggt gcg ctg ggt ctt ggc cat gat tcc cgt cgt gcg gtg gcc acc acg 595

Gly Ala Leu Gly Leu Gly His Asp Ser Arg Arg Ala Val Ala Thr Thr

150 155 160 165

gat cct gag ttc tac aag tgg act cag tgg atc ttc ctg cag att ttc 643

Asp Pro Glu Phe Tyr Lys Trp Thr Gln Trp Ile Phe Leu Gln Ile Phe

170 175 180

aat tcg tgg ttc gat gca gag cag cag aag gca cgt ccc atc agt gag 691

Asn Ser Trp Phe Asp Ala Glu Gln Gln Lys Ala Arg Pro Ile Ser Glu

185 190 195

ctg att ccg ttg ctg gag tcc ggc gag ctg aag act aag gac ggg gcg 739

Leu Ile Pro Leu Leu Glu Ser Gly Glu Leu Lys Thr Lys Asp Gly Ala

200 205 210

gat tac aac gcg ctg gga gac gtc gaa aag caa aaa gcg gtg gat gac 787

Asp Tyr Asn Ala Leu Gly Asp Val Glu Lys Gln Lys Ala Val Asp Asp

215 220 225

tac cgc ctt gtt tat cgc tcg aac tcc acc gtg aac tgg tgc cca ggc 835

Tyr Arg Leu Val Tyr Arg Ser Asn Ser Thr Val Asn Trp Cys Pro Gly

230 235 240 245

ttg ggc acc gtg ttg gca aac gag gaa gtg acc gcg gac ggc cgt tcc 883

Leu Gly Thr Val Leu Ala Asn Glu Glu Val Thr Ala Asp Gly Arg Ser

250 255 260

gag cgt ggc aat ttc cct gtt ttc cgt aag aat ttg tcc cag tgg atg 931

Glu Arg Gly Asn Phe Pro Val Phe Arg Lys Asn Leu Ser Gln Trp Met

265 270 275

atg cgc att acc gcg tac tcg gat cgt ctg atc gat gat ctg gag ctg 979

Met Arg Ile Thr Ala Tyr Ser Asp Arg Leu Ile Asp Asp Leu Glu Leu

280 285 290

ctc gat tgg act gag aag gtc aag tcc atg cag cgt aac tgg att ggc 1027

Leu Asp Trp Thr Glu Lys Val Lys Ser Met Gln Arg Asn Trp Ile Gly

295 300 305

cgt tcc cgc ggc gct gaa gtt gat ttc agt gca gag ggc gaa acc gtc 1075

Arg Ser Arg Gly Ala Glu Val Asp Phe Ser Ala Glu Gly Glu Thr Val

310 315 320 325

acc gtg ttt acc acc cgc cca gat act ctg ttc ggc gcg acc tac atg 1123

Thr Val Phe Thr Thr Arg Pro Asp Thr Leu Phe Gly Ala Thr Tyr Met

330 335 340

gtt ctt gca cct gag cat gag ctg gtc gac gtg ctg ctg gag aag gct 1171

Val Leu Ala Pro Glu His Glu Leu Val Asp Val Leu Leu Glu Lys Ala

345 350 355

ggt tcc tac gag ggc gtt gat gcc cgt tgg acc aat ggc cag gcg agc 1219

Gly Ser Tyr Glu Gly Val Asp Ala Arg Trp Thr Asn Gly Gln Ala Ser

360 365 370

cct gcg gaa gct gtc gct gca tac cgc gcc tcc atc gcc gcg aag tcc 1267

Pro Ala Glu Ala Val Ala Ala Tyr Arg Ala Ser Ile Ala Ala Lys Ser

375 380 385

gac ctg gag cgt cag gaa aac aag gaa aag acc ggc gtc ttc ctg ggc 1315

Asp Leu Glu Arg Gln Glu Asn Lys Glu Lys Thr Gly Val Phe Leu Gly

390 395 400 405

gtt tac gcg acc aac cca gtc aac ggc gat cag atc aca gtg ttc atc 1363

Val Tyr Ala Thr Asn Pro Val Asn Gly Asp Gln Ile Thr Val Phe Ile

410 415 420

gct gac tac gtt ctg acc ggc tac ggc acc ggc gcc atc atg gcg gtt 1411

Ala Asp Tyr Val Leu Thr Gly Tyr Gly Thr Gly Ala Ile Met Ala Val

425 430 435

cct gct cac gac gag cgc gac tac gaa ttc gcc acc gtt ttg ggt ctg 1459

Pro Ala His Asp Glu Arg Asp Tyr Glu Phe Ala Thr Val Leu Gly Leu

440 445 450

cct atc aag gaa gtt gtc gca ggt ggc aac atc gaa gag gct gct ttc 1507

Pro Ile Lys Glu Val Val Ala Gly Gly Asn Ile Glu Glu Ala Ala Phe

455 460 465

acc gaa tct ggc gaa gca gtc aac tct gcg aac gac aac ggc ctg gat 1555

Thr Glu Ser Gly Glu Ala Val Asn Ser Ala Asn Asp Asn Gly Leu Asp

470 475 480 485

atc aac ggc ctt gcc aag gat gag gct att gcc aag acc atc gaa tgg 1603

Ile Asn Gly Leu Ala Lys Asp Glu Ala Ile Ala Lys Thr Ile Glu Trp

490 495 500

ttg gaa gaa aag gaa ctt ggc cgc ggc acc atc cag tac aag ctg cgc 1651

Leu Glu Glu Lys Glu Leu Gly Arg Gly Thr Ile Gln Tyr Lys Leu Arg

505 510 515

gac tgg ctg ttc gct cgc cag cgt tac tgg ggc gag cct ttc cca atc 1699

Asp Trp Leu Phe Ala Arg Gln Arg Tyr Trp Gly Glu Pro Phe Pro Ile

520 525 530

gtc tac gsc gaa aac ggc caa gca cat gct ctg cca gac tcc atg ctt 1747

Val Tyr Asp Glu Asn Gly Gln Ala His Ala Leu Pro Asp Ser Met Leu

535 540 545

cca gtc gag ctg cca gag gta gag gac tac aag cct gtc tcc ttc gac 1795

Pro Val Glu Leu Pro Glu Val Glu Asp Tyr Lys Pro Val Ser Phe Asp

550 555 560 565

cct gaa gac gca gac tcc gag cct tcc cca cca ctg gct aag gcc cgc 1843

Pro Glu Asp Ala Asp Ser Glu Pro Ser Pro Pro Leu Ala Lys Ala Arg

570 575 580

gaa tgg gtt gag gtg gaa ctc gat ctc ggc gat ggc aag aag aag tac 1891

Glu Trp Val Glu Val Glu Leu Asp Leu Gly Asp Gly Lys Lys Lys Tyr

585 590 595

acc cgc gac acc aac gtc atg cca cag tgg gca ggt tcc tcc tgg tac 1939

Thr Arg Asp Thr Asn Val Met Pro Gln Trp Ala Gly Ser Ser Trp Tyr

600 605 610

cag ctg cgc tac gtc gat cca agc aac gat gag cag ttc tgc aac atc 1987

Gln Leu Arg Tyr Val Asp Pro Ser Asn Asp Glu Gln Phe Cys Asn Ile

615 620 625

gaa aat gaa cgc tac tgg acc ggc cca cgc cca gaa acc cac gga cca 2035

Glu Asn Glu Arg Tyr Trp Thr Gly Pro Arg Pro Glu Thr His Gly Pro

630 635 640 645

aac gat cca ggc ggc gta gac ctc tac gtc ggt ggc gtc gag cac gca 2083

Asn Asp Pro Gly Gly Val Asp Leu Tyr Val Gly Gly Val Glu His Ala

650 655 660

gtt ctc cac ctg ctc tac gca cgt ttc tgg cac aag gtc ctc ttc gac 2131

Val Leu His Leu Leu Tyr Ala Arg Phe Trp His Lys Val Leu Phe Asp

665 670 675

ctg ggc cac gtc tcc tcc aag gag cca tac cgt cgc ctg tac aac cag 2179

Leu Gly His Val Ser Ser Lys Glu Pro Tyr Arg Arg Leu Tyr Asn Gln

680 685 690

ggc tac atc cag gcc ttc gcc tac acc gat tcc cgt ggc gtc tac gtg 2227

Gly Tyr Ile Gln Ala Phe Ala Tyr Thr Asp Ser Arg Gly Val Tyr Val

695 700 705

cct gcc gat gat gtc gaa gag aag gac gga aag ttc ttc tac cag ggc 2275

Pro Ala Asp Asp Val Glu Glu Lys Asp Gly Lys Phe Phe Tyr Gln Gly

710 715 720 725

gaa gaa gtc aac cag gaa tac gga aag atg ggc aag tcc ctg aag aac 2323

Glu Glu Val Asn Gln Glu Tyr Gly Lys Met Gly Lys Ser Leu Lys Asn

730 735 740

gcc gtt gcc cca gac gat atc tgc aac aac ttc ggt gct gac acc ctg 2371

Ala Val Ala Pro Asp Asp Ile Cys Asn Asn Phe Gly Ala Asp Thr Leu

745 750 755

cgc gtt tac gag atg gcc atg gga cct ttg gac acc tcc cgt cca tgg 2419

Arg Val Tyr Glu Met Ala Met Gly Pro Leu Asp Thr Ser Arg Pro Trp

760 765 770

gca acc aag gac gtc gtc ggt gcg cag cgc ttc ctc cag cgt ctg tgg 2467

Ala Thr Lys Asp Val Val Gly Ala Gln Arg Phe Leu Gln Arg Leu Trp

775 780 785

cgt ctc gtc gtc gat gaa aac acc ggc gaa gtg ctc act cgc gat gaa 2515

Arg Leu Val Val Asp Glu Asn Thr Gly Glu Val Leu Thr Arg Asp Glu

790 795 800 805

gtc ctc acc gac gat gac aac aag caa ctg cac cgc acc atc gca ggc 2563

Val Leu Thr Asp Asp Asp Asn Lys Gln Leu His Arg Thr Ile Ala Gly

810 815 820

gtc cgc gac gac tac acc aac ttg cgc gtt aac acc gtg gtt gcc aag 2611

Val Arg Asp Asp Tyr Thr Asn Leu Arg Val Asn Thr Val Val Ala Lys

825 830 835

ctc atc gaa tac gtc aac tac ctg acc aaa aca tac cca gac acc atc 2659

Leu Ile Glu Tyr Val Asn Tyr Leu Thr Lys Thr Tyr Pro Asp Thr Ile

840 845 850

cca gct ggc gca gtc ctg cca ctg atc gtc atg gtc tcc cct atc gca 2707

Pro Ala Gly Ala Val Leu Pro Leu Ile Val Met Val Ser Pro Ile Ala

855 860 865

cca cac atc gcg gag gaa ctc tgg aag aag ctc ggc cac gac gac acc 2755

Pro His Ile Ala Glu Glu Leu Trp Lys Lys Leu Gly His Asp Asp Thr

870 875 880 885

gtc acc tac gaa cca ttc ccc acc ttt gag gaa aaa tgg ctc acc gac 2803

Val Thr Tyr Glu Pro Phe Pro Thr Phe Glu Glu Lys Trp Leu Thr Asp

890 895 900

gat gaa atc gaa ctg cca gtc cag gtc aac ggc aag gtc cgc ggt cgc 2851

Asp Glu Ile Glu Leu Pro Val Gln Val Asn Gly Lys Val Arg Gly Arg

905 910 915

atc acc gtt gca gcc gac gcc agc cag gag cag gtc atc gag gca gcg 2899

Ile Thr Val Ala Ala Asp Ala Ser Gln Glu Gln Val Ile Glu Ala Ala

920 925 930

ctt gcc gac gag aag gtg cag gag caa atc tcc ggc aag aac ctg atc 2947

Leu Ala Asp Glu Lys Val Gln Glu Gln Ile Ser Gly Lys Asn Leu Ile

935 940 945

aag cag atc gtt gtt cca gga cgc atg gtt aac ctt gtg gtg aag 2992

Lys Gln Ile Val Val Pro Gly Arg Met Val Asn Leu Val Val Lys

950 955 960

taatccccct cggtttagat tcccctagaa 3022

<210>20

<211>964

<212>PRT

<213>谷氨酸棒状杆菌

<400>20

Met Arg Cys Pro Val Tyr Leu Leu His Cys Leu Thr Met Thr Asn Pro

1 5 10 15

Ser Glu Gly Thr Thr Pro Leu Ala Phe Arg Tyr Thr Pro Glu Leu Ala

20 25 30

Asn Lys Ile Glu Gly Glu Trp Gln Asn Tyr Trp Thr Asp Asn Gly Thr

35 40 45

Phe Asn Ala Pro Asn Pro Val Gly Asp Leu Ala Pro Ala Asp Gly Lys

50 55 60

Ala Leu Pro Glu Asp Lys Leu Phe Val Gln Asp Met Phe Pro Tyr Pro

65 70 75 80

Ser Gly Ala Gly Leu His Val Gly His Pro Leu Gly Tyr Ile Ala Thr

85 90 95

Asp Val Phe Ala Arg Tyr Asn Arg Met Leu Gly Lys Asn Val Leu His

100 105 110

Thr Leu Gly Tyr Asp Ala Phe Gly Leu Pro Ala Glu Gln Tyr Ala Ile

115 120 125

Gln Thr Gly Thr His Pro Arg Thr Thr Thr Met Ala Asn Ile Glu Asn

130 135 140

Met Lys Arg Gln Leu Gly Ala Leu Gly Leu Gly His Asp Ser Arg Arg

145 150 155 160

Ala Val Ala Thr Thr Asp Pro Glu Phe Tyr Lys Trp Thr Gln Trp Ile

165 170 175

Phe Leu Gln Ile Phe Asn Ser Trp Phe Asp Ala Glu Gln Gln Lys Ala

180 185 190

Arg Pro Ile Ser Glu Leu Ile Pro Leu Leu Glu Ser Gly Glu Leu Lys

195 200 205

Thr Lys Asp Gly Ala Asp Tyr Asn Ala Leu Gly Asp Val Glu Lys Gln

210 215 220

Lys Ala Val Asp Asp Tyr Arg Leu Val Tyr Arg Ser Asn Ser Thr Val

225 230 235 240

Asn Trp Cys Pro Gly Leu Gly Thr Val Leu Ala Asn Glu Glu Val Thr

245 250 255

Ala Asp Gly Arg Ser Glu Arg Gly Asn Phe Pro Val Phe Arg Lys Asn

260 265 270

Leu Ser Gln Trp Met Met Arg Ile Thr Ala Tyr Ser Asp Arg Leu Ile

275 280 285

Asp Asp Leu Glu Leu Leu Asp Trp Thr Glu Lys Val Lys Ser Met Gln

290 295 300

Arg Asn Trp Ile Gly Arg Ser Arg Gly Ala Glu Val Asp Phe Ser Ala

305 310 315 320

Glu Gly Glu Thr Val Thr Val Phe Thr Thr Arg Pro Asp Thr Leu Phe

325 330 335

Gly Ala Thr Tyr Met Val Leu Ala Pro Glu His Glu Leu Val Asp Val

340 345 350

Leu Leu Glu Lys Ala Gly Ser Tyr Glu Gly Val Asp Ala Arg Trp Thr

355 360 365

Asn Gly Gln Ala Ser Pro Ala Glu Ala Val Ala Ala Tyr Arg Ala Ser

370 375 380

Ile Ala Ala Lys Ser Asp Leu Glu Arg Gln Glu Asn Lys Glu Lys Thr

385 390 395 400

Gly Val Phe Leu Gly Val Tyr Ala Thr Asn Pro Val Asn Gly Asp Gln

405 410 415

Ile Thr Val Phe Ile Ala Asp Tyr Val Leu Thr Gly Tyr Gly Thr Gly

420 425 430

Ala Ile Met Ala Val Pro Ala His Asp Glu Arg Asp Tyr Glu Phe Ala

435 440 445

Thr Val Leu Gly Leu Pro Ile Lys Glu Val Val Ala Gly Gly Asn Ile

450 455 460

Glu Glu Ala Ala Phe Thr Glu Ser Gly Glu Ala Val Asn Ser Ala Asn

465 470 475 480

Asp Asn Gly Leu Asp Ile Asn Gly Leu Ala Lys Asp Glu Ala Ile Ala

485 490 495

Lys Thr Ile Glu Trp Leu Glu Glu Lys Glu Leu Gly Arg Gly Thr Ile

500 505 510

Gln Tyr Lys Leu Arg Asp Trp Leu Phe Ala Arg Gln Arg Tyr Trp Gly

515 520 525

Glu Pro Phe Pro Ile Val Tyr Asp Glu Asn Gly Gln Ala His Ala Leu

530 535 540

Pro Asp Ser Met Leu Pro Val Glu Leu Pro Glu Val Glu Asp Tyr Lys

545 550 555 560

Pro Val Ser Phe Asp Pro Glu Asp Ala Asp Ser Glu Pro Ser Pro Pro

565 570 575

Leu Ala Lys Ala Arg Glu Trp Val Glu Val Glu Leu Asp Leu Gly Asp

580 585 590

Gly Lys Lys Lys Tyr Thr Arg Asp Thr Asn Val Met Pro Gln Trp Ala

595 600 605

Gly Ser Ser Trp Tyr Gln Leu Arg Tyr Val Asp Pro Ser Asn Asp Glu

610 615 620

Gln Phe Cys Asn Ile Glu Asn Glu Arg Tyr Trp Thr Gly Pro Arg Pro

625 630 635 640

Glu Thr His Gly Pro Asn Asp Pro Gly Gly Val Asp Leu Tyr Val Gly

645 650 655

Gly Val Glu His Ala Val Leu His Leu Leu Tyr Ala Arg Phe Trp His

660 665 670

Lys Val Leu Phe Asp Leu Gly His Val Ser Ser Lys Glu Pro Tyr Arg

675 680 685

Arg Leu Tyr Asn Gln Gly Tyr Ile Gln Ala Phe Ala Tyr Thr Asp Ser

690 695 700

Arg Gly Val Tyr Val Pro Ala Asp Asp Val Glu Glu Lys Asp Gly Lys

705 710 715 720

Phe Phe Tyr Gln Gly Glu Glu Val Asn Gln Glu Tyr Gly Lys Met Gly

725 730 735

Lys Ser Leu Lys Asn Ala Val Ala Pro Asp Asp Ile Cys Asn Asn Phe

740 745 750

Gly Ala Asp Thr Leu Arg Val Tyr Glu Met Ala Met Gly Pro Leu Asp

755 760 765

Thr Ser Arg Pro Trp Ala Thr Lys Asp Val Val Gly Ala Gln Arg Phe

770 775 780

Leu Gln Arg Leu Trp Arg Leu Val Val Asp Glu Asn Thr Gly Glu Val

785 790 795 800

Leu Thr Arg Asp Glu Val Leu Thr Asp Asp Asp Asn Lys Gln Leu His

805 810 815

Arg Thr Ile Ala Gly Val Arg Asp Asp Tyr Thr Asn Leu Arg Val Asn

820 825 830

Thr Val Val Ala Lys Leu Ile Glu Tyr Val Asn Tyr Leu Thr Lys Thr

835 840 845

Tyr Pro Asp Thr Ile Pro Ala Gly Ala Val Leu Pro Leu Ile Val Met

850 855 860

Val Ser Pro Ile Ala Pro His Ile Ala Glu Glu Leu Trp Lys Lys Leu

865 870 875 880

Gly His Asp Asp Thr Val Thr Tyr Glu Pro Phe Pro Thr Phe Glu Glu

885 890 895

Lys Trp Leu Thr Asp Asp Glu Ile Glu Leu Pro Val Gln Val Asn Gly

900 905 910

Lys Val Arg Gly Arg Ile Thr Val Ala Ala Asp Ala Ser Gln Glu Gln

915 920 925

Val Ile Glu Ala Ala Leu Ala Asp Glu Lys Val Gln Glu Gln Ile Ser

930 935 940

Gly Lys Asn Leu Ile Lys Gln Ile Val Val Pro Gly Arg Met Val Asn

945 950 955 960

Leu Val Val Lys

<210>21

<211>2248

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2218)

<223>RXA01277

<400>21

gaccagccga atctacattc cttattctgc tggcgttaca attcagggcc aaacccgtat 60

gatgaaaaag acaccgggga aatcggagtg cgcgtagatt ttg aaa acg gcc ggt 115

Leu Lys Thr Ala Gly

1 5

act act cgg ttc acg ttt acg tcg gct gat cca att gga ggc gcc ctc 163

Thr Thr Arg Phe Thr Phe Thr Ser Ala Asp Pro Ile Gly Gly Ala Leu

10 15 20

gga agc cgc ctt aaa aaa cct gcc ggt caa aag atc act aac ctg aac 211

Gly Ser Arg Leu Lys Lys Pro Ala Gly Gln Lys Ile Thr Asn Leu Asn

25 30 35

ttc atg act gat tac acg ttc ctc gaa gac att gac acc ccg gaa gcg 259

Phe Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala

40 45 50

ctc gcg tgg gcg gaa aaa tgg tcg ggg gaa agc gtc gaa aag cta aaa 307

Leu Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser Val Glu Lys Leu Lys

55 60 65

agc cca gcc aag gac gcc ctg gaa gcc agg ctg ctg gct gcg ttg gac 355

Ser Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp

70 75 80 85

acc gat gat cgc att gcc tac gtg agc cgg cgc ggt gag aag ctg tac 403

Thr Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr

90 95 100

aac ttt tgg cgg gac gcg cag cat ccg cgt gga gtg tgg cgc acg acc 451

Asn Phe Trp Arg Asp Ala Gln His Pro Arg Gly Val Trp Arg Thr Thr

105 110 115

acg ttg gag tcg tat gaa agt gac cag ccg gag tgg gac gtg ctc att 499

Thr Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu Trp Asp Val Leu Ile

120 125 130

gat gtg gat gcg ttg gcg gag gat gag ggc gaa aac tgg gta tgg aag 547

Asp Val Asp Ala Leu Ala Glu Asp Glu Gly Glu Asn Trp Val Trp Lys

135 140 145

ggc gcg gtt gtg cgc tcg ccg gag ttt gat cgg gcg ttg gtg aag ttc 595

Gly Ala Val Val Arg Ser Pro Glu Phe Asp Arg Ala Leu Val Lys Phe

150 155 160 165

tcg cgg ggc ggg gct gat gcg acg gtg att agg gag ttt gat ctg gcc 643

Ser Arg Gly Gly Ala Asp Ala Thr Val Ile Arg Glu Phe Asp Leu Ala

170 175 180

acg gct gct ttc gtg gat gat tcg ccg ttt gaa ttg gag gag gcg aag 691

Thr Ala Ala Phe Val Asp Asp Ser Pro Phe Glu Leu Glu Glu Ala Lys

185 190 195

tcc gat gtc acg tgg gtt gat ctg gat acg ttg ctg gtg ggc acg gat 739

Ser Asp Val Thr Trp Val Asp Leu Asp Thr Leu Leu Val Gly Thr Asp

200 205 210

acc ggc gag ggg tca ctg acg gat tct ggg tac ccg gcg cgg gtg ctc 787

Thr Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu

215 220 225

acg tgg aag cgt ggg act ccg ctt gag cag gcg gag ttg ttc ttt gag 835

Thr Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu

230 235 240 245

ggg tcg cgt cag gat gtg gcg act cat gcg tgg cgg gat tca aca cct 883

Gly Ser Arg Gln Asp Val Ala Thr His Ala Trp Arg Asp Ser Thr Pro

250 255 260

ggt ttt gag cgg acg ttt gtg tca agg tcg ttg gat ttc tat aat tcg 931

Gly Phe Glu Arg Thr Phe Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser

265 270 275

gag acg tcg ctg gaa acc gag ggt ggc ctg gtc aag ctt gat gtg ccg 979

Glu Thr Ser Leu Glu Thr Glu Gly Gly Leu Val Lys Leu Asp Val Pro

280 285 290

acc gat tgc gat gtc att gtg aag aag cag tgg att ttt gtg agt cct 1027

Thr Asp Cys Asp Val Ile Val Lys Lys Gln Trp Ile Phe Val Ser Pro

295 300 305

cgg acg gat ttc gct ggg att cca gca ggt ggc ttg gga gtg ctg ctg 1075

Arg Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly Leu Gly Val Leu Leu

310 315 320 325

tta aag gag ttc ctt gag ggc ggg cgc gat ttt cag cct gtg ttt acg 1123

Leu Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe Gln Pro Val Phe Thr

330 335 340

cct act gag tcg acg tcg ctg cag gga ttg gcc acg aca aag aat ttc 1171

Pro Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe

345 350 355

ctg gtt tta acg ctc ctt aat aat gtc tcc aca gaa atc gtc aca gtg 1219

Leu Val Leu Thr Leu Leu Asn Asn Val Ser Thr Glu Ile Val Thr Val

360 365 370

ccg ctc aat gat ccg aca acg gag cat gaa cac att gac ctc cca gag 1267

Pro Leu Asn Asp Pro Thr Thr Glu His Glu His Ile Asp Leu Pro Glu

375 380 385

cat gtc acc gcg cat gtg gtt gct acc tcc ccg ttg gat ggc gat gaa 1315

His Val Thr Ala His Val Val Ala Thr Ser Pro Leu Asp Gly Asp Glu

390 395 400 405

att tgg gtg cag gca gcg agt ttc acc gaa gcg cca acg ttg ctg cgt 1363

Ile Trp Val Gln Ala Ala Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg

410 415 420

gcg gag ctg cct ggt gcg ctt gag gct gtg aag aag gcg ccg ttg cag 1411

Ala Glu Leu Pro Gly Ala Leu Glu Ala Val Lys Lys Ala Pro Leu Gln

425 430 435

ttt gaa aat gct ggt cag gag act cgt cag cat tgg gca acc tcg gcg 1459

Phe Glu Asn Ala Gly Gln Glu Thr Arg Gln His Trp Ala Thr Ser Ala

440 445 450

gat gga acg aag att ccg tac ttt att aca gga gcc ttc gag gag gaa 1507

Asp Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly Ala Phe Glu Glu Glu

455 460 465

cca caa aac acc ctg gtc cac gcc tac ggc ggc ttc gag gtt tcc ctt 1555

Pro Gln Asn Thr Leu Val His Ala Tyr Gly Gly Phe Glu Val Ser Leu

470 475 480 485

acc cca agc cac tcc ccg acc cgc ggc atc gca tgg ttg gaa aag ggc 1603

Thr Pro Ser His Ser Pro Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly

490 495 500

tac tac ttt gtg gaa gcc aac ctg cgt ggt ggc ggt gaa ttc ggt ccg 1651

Tyr Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro

505 510 515

gaa tgg cat tcg cag gca acc aag ctg aac cgc atg aag gtg tgg gag 1699

Glu Trp His Ser Gln Ala Thr Lys Leu Asn Arg Met Lys Val Trp Glu

520 525 530

gat cac cgc gcg gtg ctc gcc gac ctt gtg gag cgc ggc tac gca acg 1747

Asp His Arg Ala Val Leu Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr

535 540 545

ccg gag cag att gcg att cgt ggc gga tcc aac ggt ggt ttg ctg aca 1795

Pro Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr

550 555 560 565

agt ggc gcg tta act cag tac cca gaa gca ttc ggt gcg gca gtt gtg 1843

Ser Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe Gly Ala Ala Val Val

570 575 580

cag gtg ccg ttg gct gat atg ttg cgc tat cac acc tgg tca gcg ggt 1891

Gln Val Pro Leu Ala Asp Met Leu Arg Tyr His Thr Trp Ser Ala Gly

585 590 595

gct tcg tgg atg gcg gag tac ggc aac cct gac gat ccg gag gaa cgg 1939

Ala Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp Asp Pro Glu Glu Arg

600 605 610

gcg gtg att gag cag tac tcg ccg gtg cag gcg gtg gtg ggc gtc gag 1987

Ala Val Ile Glu Gln Tyr Ser Pro Val Gln Ala Val Val Gly Val Glu

615 620 625

aag cga att tat cca ccc gca ttg gtg acg acc tca acc cgg gac gac 2035

Lys Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr Ser Thr Arg Asp Asp

630 635 640 645

cgc gtc cac ccc gcg cac gcg cgc ctt ttt gct caa gct ttg ctt gat 2083

Arg Val His Pro Ala His Ala Arg Leu Phe Ala Gln Ala Leu Leu Asp

650 655 660

gcg ggc cag gcc gtg gat tac tac gaa aac acc gag ggc ggc cat gcc 2131

Ala Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr Glu Gly Gly His Ala

665 670 675

ggc gcg gcg gat aac aag cag acc gcg ttt gtg gaa tcg ctg atc tac 2179

Gly Ala Ala Asp Asn Lys Gln Thr Ala Phe Val Glu Ser Leu Ile Tyr

680 685 690

acc tgg atc gag aag act ttg gat cag cag ggt agc att taatacctat 2228

Thr Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly Ser Ile

695 700 705

gattatgcga aggctgcgct 2248

<210>22

<211>706

<212>PRT

<213>谷氨酸棒状杆菌

<400>22

Leu Lys Thr Ala Gly Thr Thr Arg Phe Thr Phe Thr Ser Ala Asp Pro

1 5 10 15

Ile Gly Gly Ala Leu Gly Ser Arg Leu Lys Lys Pro Ala Gly Gln Lys

20 25 30

Ile Thr Asn Leu Asn Phe Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile

35 40 45

Asp Thr Pro Glu Ala Leu Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser

50 55 60

Val Glu Lys Leu Lys Ser Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu

65 70 75 80

Leu Ala Ala Leu Asp Thr Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg

85 90 95

Gly Glu Lys Leu Tyr Asn Phe Trp Arg Asp Ala Gln His Pro Arg Gly

100 105 110

Val Trp Arg Thr Thr Thr Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu

115 120 125

Trp Asp Val Leu Ile Asp Val Asp Ala Leu Ala Glu Asp Glu Gly Glu

130 135 140

Asn Trp Val Trp Lys Gly Ala Val Val Arg Ser Pro Glu Phe Asp Arg

145 150 155 160

Ala Leu Val Lys Phe Ser Arg Gly Gly Ala Asp Ala Thr Val Ile Arg

165 170 175

Glu Phe Asp Leu Ala Thr Ala Ala Phe Val Asp Asp Ser Pro Phe Glu

180 185 190

Leu Glu Glu Ala Lys Ser Asp Val Thr Trp Val Asp Leu Asp Thr Leu

195 200 205

Leu Val Gly Thr Asp Thr Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr

210 215 220

Pro Ala Arg Val Leu Thr Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala

225 230 235 240

Glu Leu Phe Phe Glu Gly Ser Arg Gln Asp Val Ala Thr His Ala Trp

245 250 255

Arg Asp Ser Thr Pro Gly Phe Glu Arg Thr Phe Val Ser Arg Ser Leu

260 265 270

Asp Phe Tyr Asn Ser Glu Thr Ser Leu Glu Thr Glu Gly Gly Leu Val

275 280 285

Lys Leu Asp Val Pro Thr Asp Cys Asp Val Ile Val Lys Lys Gln Trp

290 295 300

Ile Phe Val Ser Pro Arg Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly

305 310 315 320

Leu Gly Val Leu Leu Leu Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe

325 330 335

Gln Pro Val Phe Thr Pro Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala

340 345 350

Thr Thr Lys Asn Phe Leu Val Leu Thr Leu Leu Asn Asn Val Ser Thr

355 360 365

Glu Ile Val Thr Val Pro Leu Asn Asp Pro Thr Thr Glu His Glu His

370 375 380

Ile Asp Leu Pro Glu His Val Thr Ala His Val Val Ala Thr Ser Pro

385 390 395 400

Leu Asp Gly Asp Glu Ile Trp Val Gln Ala Ala Ser Phe Thr Glu Ala

405 410 415

Pro Thr Leu Leu Arg Ala Glu Leu Pro Gly Ala Leu Glu Ala Val Lys

420 425 430

Lys Ala Pro Leu Gln Phe Glu Asn Ala Gly Gln Glu Thr Arg Gln His

435 440 445

Trp Ala Thr Ser Ala Asp Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly

450 455 460

Ala Phe Glu Glu Glu Pro Gln Asn Thr Leu Val His Ala Tyr Gly Gly

465 470 475 480

Phe Glu Val Ser Leu Thr Pro Ser His Ser Pro Thr Arg Gly Ile Ala

485 490 495

Trp Leu Glu Lys Gly Tyr Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly

500 505 510

Gly Glu Phe Gly Pro Glu Trp His Ser Gln Ala Thr Lys Leu Asn Arg

515 520 525

Met Lys Val Trp Glu Asp His Arg Ala Val Leu Ala Asp Leu Val Glu

530 535 540

Arg Gly Tyr Ala Thr Pro Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn

545 550 555 560

Gly Gly Leu Leu Thr Ser Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe

565 570 575

Gly Ala Ala Val Val Gln Val Pro Leu Ala Asp Met Leu Arg Tyr His

580 585 590

Thr Trp Ser Ala Gly Ala Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp

595 600 605

Asp Pro Glu Glu Arg Ala Val Ile Glu Gln Tyr Ser Pro Val Gln Ala

610 615 620

Val Val Gly Val Glu Lys Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr

625 630 635 640

Ser Thr Arg Asp Asp Arg Val His Pro Ala His Ala Arg Leu Phe Ala

645 650 655

Gln Ala Leu Leu Asp Ala Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr

660 665 670

Glu Gly Gly His Ala Gly Ala Ala Asp Asn Lys Gln Thr Ala Phe Val

675 680 685

Glu Ser Leu Ile Tyr Thr Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly

690 695 700

Ser Ile

705

<210>23

<211>2257

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2227)

<223>RXA01278

<400>23

ttatccgtag gtgacaaact ttttaatact tgggtatctg tcatggatac cccggtaata 60

aataagtgaa ttaccgtaac caacaagttg gggtaccact gtg gca caa gaa gtg 115

Val Ala Gln Glu Val

1 5

ctt aag gat cta aac aag gtc cgc aac atc ggc atc atg gcg cac atc 163

Leu Lys Asp Leu Asn Lys Val Arg Asn Ile Gly Ile Met Ala His Ile

10 15 20

gat gct ggt aag acc acg acc acc gaa cgc atc ctc ttc tac acc ggc 211

Asp Ala Gly Lys Thr Thr Thr Thr Glu Arg Ile Leu Phe Tyr Thr Gly

25 30 35

atc aac cgt aag gtc ggt gag acc cac gac ggt ggc gca acc acc gac 259

Ile Asn Arg Lys Val Gly Glu Thr His Asp Gly Gly Ala Thr Thr Asp

40 45 50

tgg atg gag cag gag aag gaa cgc ggc atc acc att acc tcc gcc gcg 307

Trp Met Glu Gln Glu Lys Glu Arg Gly Ile Thr Ile Thr Ser Ala Ala

55 60 65

gtt acc tgt ttc tgg gat aac aac cag gtc aac atc att gac acc cct 355

Val Thr Cys Phe Trp Asp Asn Asn Gln Val Asn Ile Ile Asp Thr Pro

70 75 80 85

ggc cac gtt gac ttc acc gtt gag gtt gag cgt tcc ctc cgc gtg ctt 403

Gly His Val Asp Phe Thr Val Glu Val Glu Arg Ser Leu Arg Val Leu

90 95 100

gac ggc gca gtt gct gtg ttc gac ggc aag gaa ggc gtt gag cca cag 451

Asp Gly Ala Val Ala Val Phe Asp Gly Lys Glu Gly Val Glu Pro Gln

105 110 115

tct gag cag gtt tgg cgt cag gct acc aag tac gac gtt cca cgt atc 499

Ser Glu Gln Val Trp Arg Gln Ala Thr Lys Tyr Asp Val Pro Arg Ile

120 125 130

tgc ttc gtg aac aag atg gac aag ctc ggt gct gac ttc tac ttc acc 547

Cys Phe Val Asn Lys Met Asp Lys Leu Gly Ala Asp Phe Tyr Phe Thr

135 140 145

gtt ggc acc atc gag gac cgc ctg ggt gca aag cca ttg gtt atg cag 595

Val Gly Thr Ile Glu Asp Arg Leu Gly Ala Lys Pro Leu Val Met Gln

150 155 160 165

ctc cca atc ggt gct gag gac aac ttc gac ggc gtc atc gac ctt ctt 643

Leu Pro Ile Gly Ala Glu Asp Asn Phe Asp Gly Val Ile Asp Leu Leu

170 175 180

gaa atg aag gca ctg acc tgg cgt gga gtt acc cca att ggt acc gaa 691

Glu Met Lys Ala Leu Thr Trp Arg Gly Val Thr Pro Ile Gly Thr Glu

185 190 195

gct acc gtt gag gag atc cca gca gag ctc gca gac cgc gca gct gag 739

Ala Thr Val Glu Glu Ile Pro Ala Glu Leu Ala Asp Arg Ala Ala Glu

200 205 210

tac cgt gag aag ctt ctc gag acc gtt gca gag tcc gac gaa gag ctc 787

Tyr Arg Glu Lys Leu Leu Glu Thr Val Ala Glu Ser Asp Glu Glu Lau

215 220 225

atg gag aag tac ttc ggt ggc gaa gag ctc agc atc gct gag atc aag 835

Met Glu Lys Tyr Phe Gly Gly Glu Glu Leu Ser Ile Ala Glu Ile Lys

230 235 240 245

gca gct atc cgt aag atg gtt gtt aac tct gag atc tac cct gtt tac 883

Ala Ala Ile Arg Lys Met Val Val Asn Ser Glu Ile Tyr Pro Val Tyr

250 255 260

tgt ggc acc gcc tac aag aac aag ggc atc cag cca ctg ctc gac gca 931

Cys Gly Thr Ala Tyr Lys Asn Lys Gly Ile Gln Pro Leu Leu Asp Ala

265 270 275

gtc gtt gac ttc ctg cct tcc cca ctg gat ctc ggc gag acc aag ggc 979

Val Val Asp Phe Leu Pro Ser Pro Leu Asp Leu Gly Glu Thr Lys Gly

280 285 290

act gac gtt aag gat cct gag aag gtt ctg acc cgt aag cct tcc gac 1027

Thr Asp Val Lys Asp Pro Glu Lys Val Leu Thr Arg Lys Pro Ser Asp

295 300 305

gaa gag cca ctg tct gca ctt gca ttc aag att gca gct cac cca ttc 1075

Glu Glu Pro Leu Ser Ala Leu Ala Phe Lys Ile Ala Ala His Pro Phe

310 315 320 325

ttc ggt aag ctg acc ttc gtt cgt ctg tac tcc ggc aag gtt gag cca 1123

Phe Gly Lys Leu Thr Phe Val Arg Leu Tyr Ser Gly Lys Val Glu Pro

330 335 340

ggc gag cag gtt ctt aac tcc acc aag aac aag aag gaa cgc att ggt 1171

Gly Glu Gln Val Leu Asn Ser Thr Lys Asn Lys Lys Glu Arg Ile Gly

345 350 355

aag ctg ttc cag atg cac gcc aac aag gaa aac cct gtt gag gtt gca 1219

Lys Leu Phe Gln Met His Ala Asn Lys Glu Asn Pro Val Glu Val Ala

360 365 370

cac gct ggt aac atc tac gcg ttc atc ggc ctg aag gac acc acc acc 1267

His Ala Gly Asn Ile Tyr Ala Phe Ile Gly Leu Lys Asp Thr Thr Thr

375 380 385

ggt gac acc ctc tgt gac gca aac gct cca atc att ctt gag tcc atg 1315

Gly Asp Thr Leu Cys Asp Ala Asn Ala Pro Ile Ile Leu Glu Ser Met

390 395 400 405

gac ttc ccg gat cca gtt atc cag gtt gct att gag cct aag acc aag 1363

Asp Phe Pro Asp Pro Val Ile Gln Val Ala Ile Glu Pro Lys Thr Lys

410 415 420

tct gac cag gag aag ctc ggc gta gct atc cag aag ctt gct gaa gaa 1411

Ser Asp Gln Glu Lys Leu Gly Val Ala Ile Gln Lys Leu Ala Glu Glu

425 430 435

gac cca acc ttc acc gtt cac ttg gac gat gag tcc ggc cag acc gtc 1459

Asp Pro Thr Phe Thr Val His Leu Asp Asp Glu Ser Gly Gln Thr Val

440 445 450

att ggc ggc atg ggc gag ctg cac ctc gat gtt ctt gtt gac cgc atg 1507

Ile Gly Gly Met Gly Glu Leu His Leu Asp Val Leu Val Asp Arg Met

455 460 465

aag cgc gag ttc aag gtt gag gca aac atc ggt gac cca cag gtt gct 1555

Lys Arg Glu Phe Lys Val Glu Ala Asn Ile Gly Asp Pro Gln Val Ala

470 475 480 485

tac cgt gag acc atc cgt aag cct gtt gag tcc ctc agc tac acc cac 1603

Tyr Arg Glu Thr Ile Arg Lys Pro Val Glu Ser Leu Ser Tyr Thr His

490 495 500

aag aag cag act ggt ggt tcc ggt cag ttc gct aag gtc atc atc acc 1651

Lys Lys Gln Thr Gly Gly Ser Gly Gln Phe Ala Lys Val Ile Ile Thr

505 510 515

att gag cct tac gca cct gag gca gac gag ctt gaa gag ggc gag tcc 1699

Ile Glu Pro Tyr Ala Pro Glu Ala Asp Glu Leu Glu Glu Gly Glu Ser

520 525 530

gca atc tac aag ttc gag aac gct gtc acc ggt ggt cgt gtt cca cgt 1747

Ala Ile Tyr Lys Phe Glu Asn Ala Val Thr Gly Gly Arg Val Pro Arg

535 540 545

gaa tac atc cca tcc gtt gac gct ggt atc cag gac gca atg cag tac 1795

Glu Tyr Ile Pro Ser Val Asp Ala Gly Ile Gln Asp Ala Met Gln Tyr

550 555 560 565

ggc ttc ctg gct ggc tac cca ctg gtt aac gtc aag gca acc ctt gaa 1843

Gly Phe Leu Ala Gly Tyr Pro Leu Val Asn Val Lys Ala Thr Leu Glu

570 575 580

gat ggc gct tac cac gac gtt gac tcc tct gaa atg gcc ttc aag ctc 1891

Asp Gly Ala Tyr His Asp Val Asp Ser Ser Glu Met Ala Phe Lys Leu

585 590 595

gcc ggt tcc cag gcg ttc aag gaa gct gtt gca aag gca aag cca gtc 1939

Ala Gly Ser Gln Ala Phe Lys Glu Ala Val Ala Lys Ala Lys Pro Val

600 605 610

ctc ctc gag cca atc atg tcc gtt gaa atc acc act cct gag gag tac 1987

Leu Leu Glu Pro Ile Met Ser Val Glu Ile Thr Thr Pro Glu Glu Tyr

615 620 625

atg ggt gaa gtc atc ggt gac gtg aac tcc cgc cgt ggc cag atc gct 2035

Met Gly Glu Val Ile Gly Asp Val Asn Ser Arg Arg Gly Gln Ile Ala

630 635 640 645

tcc atg gat gac cgt gca ggc gcc aag ctg gtt aag gct aag gtt cca 2083

Ser Met Asp Asp Arg Ala Gly Ala Lys Leu Val Lys Ala Lys Val Pro

650 655 660

ctg tct cag atg ttc ggt tac gtc ggt gac ctt cgc tct aag acc cag 2131

Leu Ser Gln Met Phe Gly Tyr Val Gly Asp Leu Arg Ser Lys Thr Gln

665 670 675

ggt cgt gca aac tac tcc atg gtc ttc gat tcc tac gct gag gtc cca 2179

Gly Arg Ala Asn Tyr Ser Met Val Phe Asp Ser Tyr Ala Glu Val Pro

680 685 690

gcc aac gtt gcc gca gat gtt att gct gag cgc aac ggc acc gct tcc 2227

Ala Asn Val Ala Ala Asp Val Ile Ala Glu Arg Asn Gly Thr Ala Ser

695 700 705

taaagatcgt ttagatccga aggaaaacgt 2257

<210>24

<211>709

<212>PRT

<213>谷氨酸棒状杆菌

<400>24

Val Ala Gln Glu Val Leu Lys Asp Leu Asn Lys Val Arg Asn Ile Gly

1 5 10 15

Ile Met Ala His Ile Asp Ala Gly Lys Thr Thr Thr Thr Glu Arg Ile

20 25 30

Leu Phe Tyr Thr Gly Ile Asn Arg Lys Val Gly Glu Thr His Asp Gly

35 40 45

Gly Ala Thr Thr Asp Trp Met Glu Gln Glu Lys Glu Arg Gly Ile Thr

50 55 60

Ile Thr Ser Ala Ala Val Thr Cys Phe Trp Asp Asn Asn Gln Val Asn

65 70 75 80

Ile Ile Asp Thr Pro Gly His Val Asp Phe Thr Val Glu Val Glu Arg

85 90 95

Ser Leu Arg Val Leu Asp Gly Ala Val Ala Val Phe Asp Gly Lys Glu

100 105 110

Gly Val Glu Pro Gln Ser Glu Gln Val Trp Arg Gln Ala Thr Lys Tyr

115 120 125

Asp Val Pro Arg Ile Cys Phe Val Asn Lys Met Asp Lys Leu Gly Ala

130 135 140

Asp Phe Tyr Phe Thr Val Gly Thr Ile Glu Asp Arg Leu Gly Ala Lys

145 150 155 160

Pro Leu Val Met Gln Leu Pro Ile Gly Ala Glu Asp Asn Phe Asp Gly

165 170 175

Val Ile Asp Leu Leu Glu Met Lys Ala Leu Thr Trp Arg Gly Val Thr

180 185 190

Pro Ile Gly Thr Glu Ala Thr Val Glu Glu Ile Pro Ala Glu Leu Ala

195 200 205

Asp Arg Ala Ala Glu Tyr Arg Glu Lys Leu Leu Glu Thr Val Ala Glu

210 215 220

Ser Asp Glu Glu Leu Met Glu Lys Tyr Phe Gly Gly Glu Glu Leu Ser

225 230 235 240

Ile Ala Glu Ile Lys Ala Ala Ile Arg Lys Met Val Val Asn Ser Glu

245 250 255

Ile Tyr Pro Val Tyr Cys Gly Thr Ala Tyr Lys Asn Lys Gly Ile Gln

260 265 270

Pro Leu Leu Asp Ala Val Val Asp Phe Leu Pro Ser Pro Leu Asp Leu

275 280 285

Gly Glu Thr Lys Gly Thr Asp Val Lys Asp Pro Glu Lys Val Leu Thr

290 295 300

Arg Lys Pro Ser Asp Glu Glu Pro Leu Ser Ala Leu Ala Phe Lys Ile

305 310 315 320

Ala Ala His Pro Phe Phe Gly Lys Leu Thr Phe Val Arg Leu Tyr Ser

325 330 335

Gly Lys Val Glu Pro Gly Glu Gln Val Leu Asn Ser Thr Lys Asn Lys

340 345 350

Lys Glu Arg Ile Gly Lys Leu Phe Gln Met His Ala Asn Lys Glu Asn

355 360 365

Pro Val Glu Val Ala His Ala Gly Asn Ile Tyr Ala Phe Ile Gly Leu

370 375 380

Lys Asp Thr Thr Thr Gly Asp Thr Leu Cys Asp Ala Asn Ala Pro Ile

385 390 395 400

Ile Leu Glu Ser Met Asp Phe Pro Asp Pro Val Ile Gln Val Ala Ile

405 410 415

Glu Pro Lys Thr Lys Ser Asp Gln Glu Lys Leu Gly Val Ala Ile Gln

420 425 430

Lys Leu Ala Glu Glu Asp Pro Thr Phe Thr Val His Leu Asp Asp Glu

435 440 445

Ser Gly Gln Thr Val Ile Gly Gly Met Gly Glu Leu His Leu Asp Val

450 455 460

Leu Val Asp Arg Met Lys Arg Glu Phe Lys Val Glu Ala Asn Ile Gly

465 470 475 480

Asp Pro Gln Val Ala Tyr Arg Glu Thr Ile Arg Lys Pro Val Glu Ser

485 490 495

Leu Ser Tyr Thr His Lys Lys Gln Thr Gly Gly Ser Gly Gln Phe Ala

500 505 510

Lys Val Ile Ile Thr Ile Glu Pro Tyr Ala Pro Glu Ala Asp Glu Leu

515 520 525

Glu Glu Gly Glu Ser Ala Ile Tyr Lys Phe Glu Asn Ala Val Thr Gly

530 535 540

Gly Arg Val Pro Arg Glu Tyr Ile Pro Ser Val Asp Ala Gly Ile Gln

545 550 555 560

Asp Ala Met Gln Tyr Gly Phe Leu Ala Gly Tyr Pro Leu Val Asn Val

565 570 575

Lys Ala Thr Leu Glu Asp Gly Ala Tyr His Asp Val Asp Ser Ser Glu

580 585 590

Met Ala Phe Lys Leu Ala Gly Ser Gln Ala Phe Lys Glu Ala Val Ala

595 600 605

Lys Ala Lys Pro Val Leu Leu Glu Pro Ile Met Ser Val Glu Ile Thr

610 615 620

Thr Pro Glu Glu Tyr Met Gly Glu Val Ile Gly Asp Val Asn Ser Arg

625 630 635 640

Arg Gly Gln Ile Ala Ser Met Asp Asp Arg Ala Gly Ala Lys Leu Val

645 650 655

Lys Ala Lys Val Pro Leu Ser Gln Met Phe Gly Tyr Val Gly Asp Leu

660 665 670

Arg Ser Lys Thr Gln Gly Arg Ala Asn Tyr Ser Met Val Phe Asp Ser

675 680 685

Tyr Ala Glu Val Pro Ala Asn Val Ala Ala Asp Val Ile Ala Glu Arg

690 695 700

Asn Gly Thr Ala Ser

705

<210>25

<211>1318

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1288)

<223>RXA01284

<400>25

atctgtgtgc tcagtcttcc aggctgctta tcacagtgaa agcaaaacca attcgtggct 60

gcgaaagtcg tagccaccac gaagtccagg aggacataca gtg gca aag gcg aag 115

Val Ala Lys Ala Lys

1 5

ttc gag cgt acc aag ccc cac gta aac atc ggc acc atc ggt cac gtt 163

Phe Glu Arg Thr Lys Pro His Val Asn Ile Gly Thr Ile Gly His Val

10 15 20

gac cac ggt aag acc acc acc acc gcg gct atc acc aag gtt ctg gct 211

Asp His Gly Lys Thr Thr Thr Thr Ala Ala Ile Thr Lys Val Leu Ala

25 30 35

gac act tac cct gag ctc aac gag gct ttc gcc ttc gac tcc atc gat 259

Asp Thr Tyr Pro Glu Leu Asn Glu Ala Phe Ala Phe Asp Ser Ile Asp

40 45 50

aag gct cct gag gag aag gag cgt ggc atc acg atc aac atc tcc cac 307

Lys Ala Pro Glu Glu Lys Glu Arg Gly Ile Thr Ile Asn Ile Ser His

55 60 65

gtt gag tac cag act gaa aag cgc cac tac gca cac gtt gac gct cca 355

Val Glu Tyr Gln Thr Glu Lys Arg His Tyr Ala His Val Asp Ala Pro

70 75 80 85

ggc cac gcc gac tac atc aag aac atg att acc ggc gct gct cag atg 403

Gly His Ala Asp Tyr Ile Lys Asn Met Ile Thr Gly Ala Ala Gln Met

90 95 100

gac ggc gca atc ctc gtt gtt gct gct acc gac ggc cca atg cct cag 451

Asp Gly Ala Ile Leu Val Val Ala Ala Thr Asp Gly Pro Met Pro Gln

105 110 115

acc cgt gag cac gtt ctt ctt gct cgc cag gtt ggc gtt cct tac atc 499

Thr Arg Glu His Val Leu Leu Ala Arg Gln Val Gly Val Pro Tyr Ile

120 125 130

ctc gtt gct ctt aac aag tgc gac atg gtt gag gat gag gaa atc atc 547

Leu Val Ala Leu Asn Lys Cys Asp Met Val Glu Asp Glu Glu Ile Ile

135 140 145

gag ctc gtc gag atg gaa gtt cgt gaa ctt ctt gct gag cag gac tac 595

Glu Leu Val Glu Met Glu Val Arg Glu Leu Leu Ala Glu Gln Asp Tyr

150 155 160 165

gac gaa gag gct cca att gtt cac atc tcc gct ctg aag gct ctt gag 643

Asp Glu Glu Ala Pro Ile Val His Ile Ser Ala Leu Lys Ala Leu Glu

170 175 180

ggc gac gag aag tgg ggc aag cag atc ctt gag ctc atg cag gct tgc 691

Gly Asp Glu Lys Trp Gly Lys Gln Ile Leu Glu Leu Met Gln Ala Cys

185 190 195

gat gac aac atc cct gac cca gtt cgt gag acc gac aag cca ttc ctc 739

Asp Asp Asn Ile Pro Asp Pro Val Arg Glu Thr Asp Lys Pro Phe Leu

200 205 210

atg cct atc gag gac atc ttc acc atc acc ggt cgt ggc acc gtt gtt 787

Met Pro Ile Glu Asp Ile Phe Thr Ile Thr Gly Arg Gly Thr Val Val

215 220 225

acc ggt cgt gtt gag cgc ggt acc ctg aac gtg aac gat gat gtt gac 835

Thr Gly Arg Val Glu Arg Gly Thr Leu Asn Val Asn Asp Asp Val Asp

230 235 240 245

atc atc ggc atc aag gag aag tcc acc tcc acc acc gtt acc ggt atc 883

Ile Ile Gly Ile Lys Glu Lys Ser Thr Ser Thr Thr Val Thr Gly Ile

250 255 260

gag atg ttc cgt aag ctt ctt gac tcc gct gag gct ggc gac aac tgt 931

Glu Met Phe Arg Lys Leu Leu Asp Ser Ala Glu Ala Gly Asp Asn Cys

265 270 275

ggt ctg ctt ctc cgt ggt atc aag cgc gaa gat gtt gag cgt ggc cag 979

Gly Leu Leu Leu Arg Gly Ile Lys Arg Glu Asp Val Glu Arg Gly Gln

280 285 290

gtt atc gtt aag cca ggc gct tac acc cct cac acc gag ttc gag ggc 1027

Val Ile Val Lys Pro Gly Ala Tyr Thr Pro His Thr Glu Phe Glu Gly

295 300 305

tct gtc tac gtt ctg tcc aag gat gaa ggt ggc cgc cac acc cca ttc 1075

Ser Val Tyr Val Leu Ser Lys Asp Glu Gly Gly Arg His Thr Pro Phe

310 315 320 325

ttc gac aac tac cgt cct cag ttc tac ttc cgc acc acc gac gtt acc 1123

Phe Asp Asn Tyr Arg Pro Gln Phe Tyr Phe Arg Thr Thr Asp Val Thr

330 335 340

ggt gtt gtg aag ctt cca gag ggc acc gag atg gtc atg cct ggc gac 1171

Gly Val Val Lys Leu Pro Glu Gly Thr Glu Met Val Met Pro Gly Asp

345 350 355

aac gtc gac atg tcc gtc acc ctg atc cag cct gtc gct atg gac gag 1219

Asn Val Asp Met Ser Val Thr Leu Ile Gln Pro Val Ala Met Asp Glu

360 365 370

ggc ctg cgt ttc gct atc cgc gaa ggc tcc cgc acc gtt ggc gct ggt 1267

Gly Leu Arg Phe Ala Ile Arg Glu Gly Ser Arg Thr Val Gly Ala Gly

375 380 385

cgt gtc acc aag atc atc aag taatttgatg ctctaactgt tgaggtcttt 1318

Arg Val Thr Lys Ile Ile Lys

390 395

<210>26

<211>396

<212>PRT

<213>谷氨酸棒状杆菌

<400>26

Val Ala Lys Ala Lys Phe Glu Arg Thr Lys Pro His Val Asn Ile Gly

1 5 10 15

Thr Ile Gly His Val Asp His Gly Lys Thr Thr Thr Thr Ala Ala Ile

20 25 30

Thr Lys Val Leu Ala Asp Thr Tyr Pro Glu Leu Asn Glu Ala Phe Ala

35 40 45

Phe Asp Ser Ile Asp Lys Ala Pro Glu Glu Lys Glu Arg Gly Ile Thr

50 55 60

Ile Asn Ile Ser His Val Glu Tyr Gln Thr Glu Lys Arg His Tyr Ala

65 70 75 80

His Val Asp Ala Pro Gly His Ala Asp Tyr Ile Lys Asn Met Ile Thr

85 90 95

Gly Ala Ala Gln Met Asp Gly Ala Ile Leu Val Val Ala Ala Thr Asp

100 105 110

Gly Pro Met Pro Gln Thr Arg Glu His Val Leu Leu Ala Arg Gln Val

115 120 125

Gly Val Pro Tyr Ile Leu Val Ala Leu Asn Lys Cys Asp Met Val Glu

130 135 140

Asp Glu Glu Ile Ile Glu Leu Val Glu Met Glu Val Arg Glu Leu Leu

145 150 155 160

Ala Glu Gln Asp Tyr Asp Glu Glu Ala Pro Ile Val His Ile Ser Ala

165 170 175

Leu Lys Ala Leu Glu Gly Asp Glu Lys Trp Gly Lys Gln Ile Leu Glu

180 185 190

Leu Met Gln Ala Cys Asp Asp Asn Ile Pro Asp Pro Val Arg Glu Thr

195 200 205

Asp Lys Pro Phe Leu Met Pro Ile Glu Asp Ile Phe Thr Ile Thr Gly

210 215 220

Arg Gly Thr Val Val Thr Gly Arg Val Glu Arg Gly Thr Leu Asn Val

225 230 235 240

Asn Asp Asp Val Asp Ile Ile Gly Ile Lys Glu Lys Ser Thr Ser Thr

245 250 255

Thr Val Thr Gly Ile Glu Met Phe Arg Lys Leu Leu Asp Ser Ala Glu

260 265 270

Ala Gly Asp Asn Cys Gly Leu Leu Leu Arg Gly Ile Lys Arg Glu Asp

275 280 285

Val Glu Arg Gly Gln Val Ile Val Lys Pro Gly Ala Tyr Thr Pro His

290 295 300

Thr Glu Phe Glu Gly Ser Val Tyr Val Leu Ser Lys Asp Glu Gly Gly

305 310 315 320

Arg His Thr Pro Phe Phe Asp Asn Tyr Arg Pro Gln Phe Tyr Phe Arg

325 330 335

Thr Thr Asp Val Thr Gly Val Val Lys Leu Pro Glu Gly Thr Glu Met

340 345 350

Val Met Pro Gly Asp Asn Val Asp Met Ser Val Thr Leu Ile Gln Pro

355 360 365

Val Ala Met Asp Glu Gly Leu Arg Phe Ala Ile Arg Glu Gly Ser Arg

370 375 380

Thr Val Gly Ala Gly Arg Val Thr Lys Ile Ile Lys

385 390 395

<210>27

<211>3625

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3595)

<223>RXA01344

<400>27

gggggatcgg gttcctcagc agaccaattg ctcaaaaata ccagcggtgt tgatctgcac 60

ttaatggcct tgaccagcca ggtgcaatta cccgcgtgag gtg ctg gaa gga ccc 115

Val Leu Glu Gly Pro

1 5

atc ttg gca gtc tcc cgc cag acc aag tca gtc gtc gat att ccc ggt 163

Ile Leu Ala Val Ser Arg Gln Thr Lys Ser Val Val Asp Ile Pro Gly

10 15 20

gca ccg cag cgt tat tct ttc gcg aag gtg tcc gca ccc att gag gtg 211

Ala Pro Gln Arg Tyr Ser Phe Ala Lys Val Ser Ala Pro Ile Glu Val

25 30 35

ccc ggg cta cta gat ctt caa ctg gat tct tac tcc tgg ctg att ggt 259

Pro Gly Leu Leu Asp Leu Gln Leu Asp Ser Tyr Ser Trp Leu Ile Gly

40 45 50

acg cct gag tgg cgt gct cgt cag aag gaa gaa ttc ggc gag gga gcc 307

Thr Pro Glu Trp Arg Ala Arg Gln Lys Glu Glu Phe Gly Glu Gly Ala

55 60 65

cgc gta acc agc ggc ctt gag aac att ctc gag gag ctc tcc cca atc 355

Arg Val Thr Ser Gly Leu Glu Asn Ile Leu Glu Glu Leu Ser Pro Ile

70 75 80 85

cag gat tac tct gga aac atg tcc ctg agc ctt tcg gag cca cgc ttc 403

Gln Asp Tyr Ser Gly Asn Met Ser Leu Ser Leu Ser Glu Pro Arg Phe

90 95 100

gaa gac gtc aag aac acc att gac gag gcg aaa gaa aag gac atc aac 451

Glu Asp Val Lys Asn Thr Ile Asp Glu Ala Lys Glu Lys Asp Ile Asn

105 110 115

tac gcg gcg cca ctg tat gtg acc gcg gag ttc gtc aac aac acc acc 499

Tyr Ala Ala Pro Leu Tyr Val Thr Ala Glu Phe Val Asn Asn Thr Thr

120 125 130

ggt gaa atc aag tct cag act gtc ttc atc ggc gat ttc cca atg atg 547

Gly Glu Ile Lys Ser Gln Thr Val Phe Ile Gly Asp Phe Pro Met Met

135 140 145

acg gac aag gga acg ttc atc atc aac gga acc gaa cgc gtt gtg gtc 595

Thr Asp Lys Gly Thr Phe Ile Ile Asn Gly Thr Glu Arg Val Val Val

150 155 160 165

agc cag ctc gtc cgc tcc ccg ggc gtg tac ttt gac cag acc atc gat 643

Ser Gln Leu Val Arg Ser Pro Gly Val Tyr Phe Asp Gln Thr Ile Asp

170 175 180

aag tca act gag cgt cca ctg cac gcc gtg aag gtt att cct tcc cgt 691

Lys Ser Thr Glu Arg Pro Leu His Ala Val Lys Val Ile Pro Ser Arg

185 190 195

ggt gct tgg ctt gag ttt gac gtc gat aag cgc gat tcg gtt ggt gtt 739

Gly Ala Trp Leu Glu Phe Asp Val Asp Lys Arg Asp Ser Val Gly Val

200 205 210

cgt att gac cgc aag cgt cgc cag cca gtc acc gta ctg ctg aag gct 787

Arg Ile Asp Arg Lys Arg Arg Gln Pro Val Thr Val Leu Leu Lys Ala

215 220 225

ctt ggc tgg acc act gag cag atc acc gag cgt ttc ggt ttc tct gaa 835

Leu Gly Trp Thr Thr Glu Gln Ile Thr Glu Arg Phe Gly Phe Ser Glu

230 235 240 245

atc atg atg tcc acc ctc gag tcc gat ggt gta gca aac acc gat gag 883

Ile Met Met Ser Thr Leu Glu Ser Asp Gly Val Ala Asn Thr Asp Glu

250 255 260

gca ttg ctg gag atc tac cgc aag cag cgt cca ggc gag cag cct acc 931

Ala Leu Leu Glu Ile Tyr Arg Lys Gln Arg Pro Gly Glu Gln Pro Thr

265 270 275

cgc gac ctt gcg cag tcc ctc ctg gac aac agc ttc ttc cgt gca aag 979

Arg Asp Leu Ala Gln Ser Leu Leu Asp Asn Ser Phe Phe Arg Ala Lys

280 285 290

cgc tac gac ctg gct cgc gtt ggt cgt tac aag atc aac cgc aag ctc 1027

Arg Tyr Asp Leu Ala Arg Val Gly Arg Tyr Lys Ile Asn Arg Lys Leu

295 300 305

ggc ctt ggt ggc gac cac gat ggt ttg atg act ctt act gaa gag gac 1075

Gly Leu Gly Gly Asp His Asp Gly Leu Met Thr Leu Thr Glu Glu Asp

310 315 320 325

atc gca acc acc atc gag tac ctg gtg cgt ctg cac gca ggt gag cgc 1123

Ile Ala Thr Thr Ile Glu Tyr Leu Val Arg Leu His Ala Gly Glu Arg

330 335 340

gtc atg act tct cca aat ggt gaa gag atc cca gtc gag acc gat gac 1171

Val Met Thr Ser Pro Asn Gly Glu Glu Ile Pro Val Glu Thr Asp Asp

345 350 355

atc gac cac ttt ggt aac cgt cgt ctg cgt acc gtt ggc gaa ctg atc 1219

Ile Asp His Phe Gly Asn Arg Arg Leu Arg Thr Val Gly Glu Leu Ile

360 365 370

cag aac cag gtc cgt gtc ggc ctg tcc cgc atg gag cgc gtt gtt cgt 1267

Gln Asn Gln Val Arg Val Gly Leu Ser Arg Met Glu Arg Val Val Arg

375 380 385

gag cgt atg acc acc cag gat gcg gag tcc att act cct act tcc ttg 1315

Glu Arg Met Thr Thr Gln Asp Ala Glu Ser Ile Thr Pro Thr Ser Leu

390 395 400 405

atc aac gtt cgt cct gtc tct gca gct atc cgt gag ttc ttc gga act 1363

Ile Asn Val Arg Pro Val Ser Ala Ala Ile Arg Glu Phe Phe Gly Thr

410 415 420

tcc cag ctg tct cag ttc atg gac cag aac aac tcc ctg tct ggt ttg 1411

Ser Gln Leu Ser Gln Phe Met Asp Gln Asn Asn Ser Leu Ser Gly Leu

425 430 435

act cac aag cgt cgt ctg tcg gct ctg ggc ccg ggt ggt ctg tcc cgt 1459

Thr His Lys Arg Arg Leu Ser Ala Leu Gly Pro Gly Gly Leu Ser Arg

440 445 450

gag cgc gcc ggc atc gag gtt cga gac gtt cac cca tct cac tac ggc 1507

Glu Arg Ala Gly Ile Glu Val Arg Asp Val His Pro Ser His Tyr Gly

455 460 465

cgt atg tgc cca att gag act ccg gaa ggt cca aac att ggc ctg atc 1555

Arg Met Cys Pro Ile Glu Thr Pro Glu Gly Pro Asn Ile Gly Leu Ile

470 475 480 485

ggt tcc ttg gct tcc tat gct cga gtg aac cca ttc ggt ttc att gag 1603

Gly Ser Leu Ala Ser Tyr Ala Arg Val Asn Pro Phe Gly Phe Ile Glu

490 495 500

acc cca tac cgt cgc atc atc gac ggc aag ctg acc gac cag att gac 1651

Thr Pro Tyr Arg Arg Ile Ile Asp Gly Lys Leu Thr Asp Gln Ile Asp

505 510 515

tac ctt acc gct gat gag gaa gac cgc ttc gtt gtt gcg cag gca aac 1699

Tyr Leu Thr Ala Asp Glu Glu Asp Arg Phe Val Val Ala Gln Ala Asn

520 525 530

acg cac tac gac gaa gag ggc aac atc acc gat gag acc gtc act gtt 1747

Thr His Tyr Asp Glu Glu Gly Asn Ile Thr Asp Glu Thr Val Thr Val

535 540 545

cgt ctg aag gac ggc gac atc gcc atg gtt ggc cgc aac gcg gtt gat 1795

Arg Leu Lys Asp Gly Asp Ile Ala Met Val Gly Arg Asn Ala Val Asp

550 555 560 565

tac atg gac gtt tcc cct cgt cag atg gtt tct gtt ggt acc gcg atg 1843

Tyr Met Asp Val Ser Pro Arg Gln Met Val Ser Val Gly Thr Ala Met

570 575 580

att cca ttc ctg gag cac gac gat gct aac cgt gca ctg atg ggc gcg 1891

Ile Pro Phe Leu Glu His Asp Asp Ala Asn Arg Ala Leu Met Gly Ala

585 590 595

aac atg cag aag cag gct gtg cca ctg att cgt gcc gag gct cct ttc 1939

Asn Met Gln Lys Gln Ala Val Pro Leu Ile Arg Ala Glu Ala Pro Phe

600 605 610

gtg ggc acc ggt atg gag cag cgc gca gca tac gac gcc ggc gac ctg 1987

Val Gly Thr Gly Met Glu Gln Arg Ala Ala Tyr Asp Ala Gly Asp Leu

615 620 625

gtt att acc cca gtc gca ggt gtg gtg gaa aac gtt tca gct gac ttc 2035

Val Ile Thr Pro Val Ala Gly Val Val Glu Asn Val Ser Ala Asp Phe

630 635 640 645

atc acc atc atg gct gat gac ggc aag cgc gaa acc tac ctg ctg cgt 2083

Ile Thr Ile Met Ala Asp Asp Gly Lys Arg Glu Thr Tyr Leu Leu Arg

650 655 660

aag ttc cag cgc acc aac cag ggc acc agc tac aac cag aag cct ttg 2131

Lys Phe Gln Arg Thr Asn Gln Gly Thr Ser Tyr Asn Gln Lys Pro Leu

665 670 675

gtt aac ttg ggc gag cgc gtt gaa gct ggc cag gtt att gct gat ggt 2179

Val Asn Leu Gly Glu Arg Val Glu Ala Gly Gln Val Ile Ala Asp Gly

680 685 690

cca ggt acc ttc aat ggt gaa atg tcc ctt ggc cgt aac ctt ctg gtt 2227

Pro Gly Thr Phe Asn Gly Glu Met Ser Leu Gly Arg Asn Leu Leu Val

695 700 705

gcg ttc atg cct tgg gaa ggc cac aac tac gag gat gcg atc atc ctc 2275

Ala Phe Met Pro Trp Glu Gly His Asn Tyr Glu Asp Ala Ile Ile Leu

710 715 720 725

aac cag aac atc gtt gag cag gac atc ttg acc tcg atc cac atc gag 2323

Asn Gln Asn Ile Val Glu Gln Asp Ile Leu Thr Ser Ile His Ile Glu

730 735 740

gag cac gag atc gat gcc cgc gac act aag ctt ggc gcc gaa gaa atc 2371

Glu His Glu Ile Asp Ala Arg Asp Thr Lys Leu Gly Ala Glu Glu Ile

745 750 755

acc cgc gac atc cct aat gtg tct gaa gaa gtc ctc aag gac ctc gac 2419

Thr Arg Asp Ile Pro Asn Val Ser Glu Glu Val Leu Lys Asp Leu Asp

760 765 770

gac cgc ggt att gtc cgc atc ggt gct gat gtt cgt gac ggc gac atc 2467

Asp Arg Gly Ile Val Arg Ile Gly Ala Asp Val Arg Asp Gly Asp Ile

775 780 785

ctg gtc ggt aag gtc acc cct aag ggc gag acc gag ctc acc ccg gaa 2515

Leu Val Gly Lys Val Thr Pro Lys Gly Glu Thr Glu Leu Thr Pro Glu

790 795 800 805

gag cgc ttg ctg cgc gca atc ttc ggt gag aag gcc cgc gaa gtt cgc 2563

Glu Arg Leu Leu Arg Ala Ile Phe Gly Glu Lys Ala Arg Glu Val Arg

810 815 820

gat acc tcc atg aag gtg cct cac ggt gag acc ggc aag gtc atc ggc 2611

Asp Thr Ser Met Lys Val Pro His Gly Glu Thr Gly Lys Val Ile Gly

825 830 835

gtg cgt cac ttc tcc cgc gag gac gac gac gat ctg gct cct ggc gtc 2659

Val Arg His Phe Ser Arg Glu Asp Asp Asp Asp Leu Ala Pro Gly Val

840 845 850

aac gag atg atc cgt atc tac gtt gct cag aag cgt aag atc cag gac 2707

Asn Glu Met Ile Arg Ile Tyr Val Ala Gln Lys Arg Lys Ile Gln Asp

855 860 865

ggc gat aag ctc gct ggc cgc cac ggt aac aag ggt gtt gtc ggt aaa 2755

Gly Asp Lys Leu Ala Gly Arg His Gly Asn Lys Gly Val Val Gly Lys

870 875 880 885

att ttg cct cag gaa gat atg cca ttc ctt cca gac ggc act cct gtt 2803

Ile Leu Pro Gln Glu Asp Met Pro Phe Leu Pro Asp Gly Thr Pro Val

890 895 900

gac atc atc ttg aac acc cac ggt gtt cca cgt cgt atg aac att ggt 2851

Asp Ile Ile Leu Asn Thr His Gly Val Pro Arg Arg Met Asn Ile Gly

905 910 915

cag gtt ctt gag acc cac ctt ggc tgg ctg gca tct gct ggt tgg tcc 2899

Gln Val Leu Glu Thr His Leu Gly Trp Leu Ala Ser Ala Gly Trp Ser

920 925 930

gtg gat cct gaa gat cct gag aac gct gag ctc gtc aag act ctg cct 2947

Val Asp Pro Glu Asp Pro Glu Asn Ala Glu Leu Val Lys Thr Leu Pro

935 940 945

gca gac ctc ctc gag gtt cct gct ggt tcc ttg act gca act cct gtg 2995

Ala Asp Leu Leu Glu Val Pro Ala Gly Ser Leu Thr Ala Thr Pro Val

950 955 960 965

ttc gac ggt gcg tca aac gaa gag ctc gca ggc ctg ctc gct aat tca 3043

Phe Asp Gly Ala Ser Asn Glu Glu Leu Ala Gly Leu Leu Ala Asn Ser

970 975 980

cgt cca aac cgc gac ggc gac gtc atg gtt aac gcg gat ggt aaa gca 3091

Arg Pro Asn Arg Asp Gly Asp Val Met Val Asn Ala Asp Gly Lys Ala

985 990 995

acg ctt atc gac ggt cgc tcc ggt gag cct tac ccg tac ccg gtt tcc 3139

Thr Leu Ile Asp Gly Arg Ser Gly Glu Pro Tyr Pro Tyr Pro Val Ser

1000 1005 1010

atc ggc tac atg tac atg ctg aag ctg cac cac ctc gtt gac gag aag 3187

Ile Gly Tyr Met Tyr Met Leu Lys Leu His His Leu Val Asp Glu Lys

1015 1020 1025

atc cac gca cgt tcc act ggt cct tac tcc atg att acc cag cag cca 3235

Ile His Ala Arg Ser Thr Gly Pro Tyr Ser Met Ile Thr Gln Gln Pro

1030 1035 1040 1045

ctg ggt ggt aaa gca cag ttc ggt gga cag cgt ttc ggc gaa atg gag 3283

Leu Gly Gly Lys Ala Gln Phe Gly Gly Gln Arg Phe Gly Glu Met Glu

1050 1055 1060

gtg tgg gca atg cag gca tac ggc gct gcc tac aca ctt cag gag ctg 3331

Val Trp Ala Met Gln Ala Tyr Gly Ala Ala Tyr Thr Leu Gln Glu Leu

1065 1070 1075

ctg acc atc aag tct gat gac gtg gtt ggc cgt gtc aag gtc tac gaa 3379

Leu Thr Ile Lys Ser Asp Asp Val Val Gly Arg Val Lys Val Tyr Glu

1080 1085 1090

gca att gtg aag ggc gag aac atc ccg gat cca ggt att cct gag tcc 3427

Ala Ile Val Lys Gly Glu Asn Ile Pro Asp Pro Gly Ile Pro Glu Ser

1095 1100 1105

ttc aag gtt ctc ctc aag gag ctc cag tcc ttg tgc ctg aac gtg gag 3475

Phe Lys Val Leu Leu Lys Glu Leu Gln Ser Leu Cys Leu Asn Val Glu

1110 1115 1120 1125

gtt ctc tcc gca gac ggc act cca atg gag ctc gcg ggt gac gac gac 3523

Val Leu Ser Ala Asp Gly Thr Pro Met Glu Leu Ala Gly Asp Asp Asp

1130 1135 1140

gac ttc gat cag gca ggc gcc tca ctt ggc atc aac ctg tcc cgt gac 3571

Asp Phe Asp Gln Ala Gly Ala Ser Leu Gly Ile Asn Leu Ser Arg Asp

1145 1150 1155

gag cgt tcc gac gcc gac acc gca tagcagatca gaaaacaacc gctagaaatc 3625

Glu Arg Ser Asp Ala Asp Thr Ala

1160 1165

<210>28

<211>1165

<212>PRT

<213>谷氨酸棒状杆菌

<400>28

Val Leu Glu Gly Pro Ile Leu Ala Val Ser Arg Gln Thr Lys Ser Val

1 5 10 15

Val Asp Ile Pro Gly Ala Pro Gln Arg Tyr Ser Phe Ala Lys Val Ser

20 25 30

Ala Pro Ile Glu Val Pro Gly Leu Leu Asp Leu Gln Leu Asp Ser Tyr

35 40 45

Ser Trp Leu Ile Gly Thr Pro Glu Trp Arg Ala Arg Gln Lys Glu Glu

50 55 60

Phe Gly Glu Gly Ala Arg Val Thr Ser Gly Leu Glu Asn Ile Leu Glu

65 70 75 80

Glu Leu Ser Pro Ile Gln Asp Tyr Ser Gly Asn Met Ser Leu Ser Leu

85 90 95

Ser Glu Pro Arg Phe Glu Asp Val Lys Asn Thr Ile Asp Glu Ala Lys

100 105 110

Glu Lys Asp Ile Asn Tyr Ala Ala Pro Leu Tyr Val Thr Ala Glu Phe

115 120 125

Val Asn Asn Thr Thr Gly Glu Ile Lys Ser Gln Thr Val Phe Ile Gly

130 135 140

Asp Phe Pro Met Met Thr Asp Lys Gly Thr Phe Ile Ile Asn Gly Thr

145 150 155 160

Glu Arg Val Val Val Ser Gln Leu Val Arg Ser Pro Gly Val Tyr Phe

165 170 175

Asp Gln Thr Ile Asp Lys Ser Thr Glu Arg Pro Leu His Ala Val Lys

180 185 190

Val Ile Pro Ser Arg Gly Ala Trp Leu Glu Phe Asp Val Asp Lys Arg

195 200 205

Asp Ser Val Gly Val Arg Ile Asp Arg Lys Arg Arg Gln Pro Val Thr

210 215 220

Val Leu Leu Lys Ala Leu Gly Trp Thr Thr Glu Gln Ile Thr Glu Arg

225 230 235 240

Phe Gly Phe Ser Glu Ile Met Met Ser Thr Leu Glu Ser Asp Gly Val

245 250 255

Ala Asn Thr Asp Glu Ala Leu Leu Glu Ile Tyr Arg Lys Gln Arg Pro

260 265 270

Gly Glu Gln Pro Thr Arg Asp Leu Ala Gln Ser Leu Leu Asp Asn Ser

275 280 285

Phe Phe Arg Ala Lys Arg Tyr Asp Leu Ala Arg Val Gly Arg Tyr Lys

290 295 300

Ile Asn Arg Lys Leu Gly Leu Gly Gly Asp His Asp Gly Leu Met Thr

305 310 315 320

Leu Thr Glu Glu Asp Ile Ala Thr Thr Ile Glu Tyr Leu Val Arg Leu

325 330 335

His Ala Gly Glu Arg Val Met Thr Ser Pro Asn Gly Glu Glu Ile Pro

340 345 350

Val Glu Thr Asp Asp Ile Asp His Phe Gly Asn Arg Arg Leu Arg Thr

355 360 365

Val Gly Glu Leu Ile Gln Asn Gln Val Arg Val Gly Leu Ser Arg Met

370 375 380

Glu Arg Val Val Arg Glu Arg Met Thr Thr Gln Asp Ala Glu Ser Ile

385 390 395 400

Thr Pro Thr Ser Leu Ile Asn Val Arg Pro Val Ser Ala Ala Ile Arg

405 410 415

Glu Phe Phe Gly Thr Ser Gln Leu Ser Gln Phe Met Asp Gln Asn Asn

420 425 430

Ser Leu Ser Gly Leu Thr His Lys Arg Arg Leu Ser Ala Leu Gly Pro

435 440 445

Gly Gly Leu Ser Arg Glu Arg Ala Gly Ile Glu Val Arg Asp Val His

450 455 460

Pro Ser His Tyr Gly Arg Met Cys Pro Ile Glu Thr Pro Glu Gly Pro

465 470 475 480

Asn Ile Gly Leu Ile Gly Ser Leu Ala Ser Tyr Ala Arg Val Asn Pro

485 490 495

Phe Gly Phe Ile Glu Thr Pro Tyr Arg Arg Ile Ile Asp Gly Lys Leu

500 505 510

Thr Asp Gln Ile Asp Tyr Leu Thr Ala Asp Glu Glu Asp Arg Phe Val

515 520 525

Val Ala Gln Ala Asn Thr His Tyr Asp Glu Glu Gly Asn Ile Thr Asp

530 535 540

Glu Thr Val Thr Val Arg Leu Lys Asp Gly Asp Ile Ala Met Val Gly

545 550 555 560

Arg Asn Ala Val Asp Tyr Met Asp Val Ser Pro Arg Gln Met Val Ser

565 570 575

Val Gly Thr Ala Met Ile Pro Phe Lau Glu His Asp Asp Ala Asn Arg

580 585 590

Ala Leu Met Gly Ala Asn Met Gln Lys Gln Ala Val Pro Leu Ile Arg

595 600 605

Ala Glu Ala Pro Phe Val Gly Thr Gly Met Glu Gln Arg Ala Ala Tyr

610 615 620

Asp Ala Gly Asp Leu Val Ile Thr Pro Val Ala Gly Val Val Glu Asn

625 630 635 640

Val Ser Ala Asp Phe Ile Thr Ile Met Ala Asp Asp Gly Lys Arg Glu

645 650 655

Thr Tyr Leu Leu Arg Lys Phe Gln Arg Thr Asn Gln Gly Thr Ser Tyr

660 665 670

Asn Gln Lys Pro Leu Val Asn Leu Gly Glu Arg Val Glu Ala Gly Gln

675 680 685

Val Ile Ala Asp Gly Pro Gly Thr Phe Asn Gly Glu Met Ser Leu Gly

690 695 700

Arg Asn Leu Leu Val Ala Phe Met Pro Trp Glu Gly His Asn Tyr Glu

705 710 715 720

Asp Ala Ile Ile Leu Asn Gln Asn Ile Val Glu Gln Asp Ile Leu Thr

725 730 735

Ser Ile His Ile Glu Glu His Glu Ile Asp Ala Arg Asp Thr Lys Leu

740 745 750

Gly Ala Glu Glu Ile Thr Arg Asp Ile Pro Asn Val Ser Glu Glu Val

755 760 765

Leu Lys Asp Leu Asp Asp Arg Gly Ile Val Arg Ile Gly Ala Asp Val

770 775 780

Arg Asp Gly Asp Ile Leu Val Gly Lys Val Thr Pro Lys Gly Glu Thr

785 790 795 800

Glu Leu Thr Pro Glu Glu Arg Leu Leu Arg Ala Ile Phe Gly Glu Lys

805 810 815

Ala Arg Glu Val Arg Asp Thr Ser Met Lys Val Pro His Gly Glu Thr

820 825 830

Gly Lys Val Ile Gly Val Arg His Phe Ser Arg Glu Asp Asp Asp Asp

835 840 845

Leu Ala Pro Gly Val Asn Glu Met Ile Arg Ile Tyr Val Ala Gln Lys

850 855 860

Arg Lys Ile Gln Asp Gly Asp Lys Leu Ala Gly Arg His Gly Asn Lys

865 870 875 880

Gly Val Val Gly Lys Ile Leu Pro Gln Glu Asp Met Pro Phe Leu Pro

885 890 895

Asp Gly Thr Pro Val Asp Ile Ile Leu Asn Thr His Gly Val Pro Arg

900 905 910

Arg Met Asn Ile Gly Gln Val Leu Glu Thr His Leu Gly Trp Leu Ala

915 920 925

Ser Ala Gly Trp Ser Val Asp Pro Glu Asp Pro Glu Asn Ala Glu Leu

930 935 940

Val Lys Thr Leu Pro Ala Asp Leu Leu Glu Val Pro Ala Gly Ser Leu

945 950 955 960

Thr Ala Thr Pro Val Phe Asp Gly Ala Ser Asn Glu Glu Leu Ala Gly

965 970 975

Leu Leu Ala Asn Ser Arg Pro Asn Arg Asp Gly Asp Val Met Val Asn

980 985 990

Ala Asp Gly Lys Ala Thr Leu Ile Asp Gly Arg Ser Gly Glu Pro Tyr

995 1000 1005

Pro Tyr Pro Val Ser Ile Gly Tyr Met Tyr Met Leu Lys Leu His His

1010 1015 1020

Leu Val Asp Glu Lys Ile His Ala Arg Ser Thr Gly Pro Tyr Ser Met

1025 1030 1035 1040

Ile Thr Gln Gln Pro Leu Gly Gly Lys Ala Gln Phe Gly Gly Gln Arg

1045 1050 1055

Phe Gly Glu Met Glu Val Trp Ala Met Gln Ala Tyr Gly Ala Ala Tyr

1060 1065 1070

Thr Leu Gln Glu Leu Leu Thr Ile Lys Ser Asp Asp Val Val Gly Arg

1075 1080 1085

Val Lys Val Tyr Glu Ala Ile Val Lys Gly Glu Asn Ile Pro Asp Pro

1090 1095 1100

Gly Ile Pro Glu Ser Phe Lys Val Leu Leu Lys Glu Leu Gln Ser Leu

1105 1110 1115 1120

Cys Leu Asn Val Glu Val Leu Ser Ala Asp Gly Thr Pro Met Glu Leu

1125 1130 1135

Ala Gly Asp Asp Asp Asp Phe Asp Gln Ala Gly Ala Ser Leu Gly Ile

1140 1145 1150

Asn Leu Ser Arg Asp Glu Arg Ser Asp Ala Asp Thr Ala

1155 1160 1165

<210>29

<211>1582

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1552)

<223>RXA01345

<400>29

cataacctca ttgaacatgc aaaactaatg cttttggggg gtatgcataa attcgtttcg 60

ttccactgca cagcccgaaa atgctgctag ggtcaagttc atg cgt ttt gga ctt 115

Met Arg Phe Gly Leu

1 5

gac ttg gga act acc cgc aca atc gcg gcc gcc gtg gac cgc gga aac 163

Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala Val Asp Arg Gly Asn

10 15 20

tat ccc atc gtc act gtg gaa gat tct tta ggc gac acc cac gat ttc 211

Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly Asp Thr His Asp Phe

25 30 35

att cca tct gtg gtg gcc ctc aag gca gat agg att gtc gcg ggt tgg 259

Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg Ile Val Ala Gly Trp

40 45 50

gat gct att gag gtt ggg cag gac cac cct tcc ttc gta cgt tct ttc 307

Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser Phe Val Arg Ser Phe

55 60 65

aaa cgc cta ctc tct gaa ccc aat gtc acg gaa gcc acc ccg gtc tac 355

Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu Ala Thr Pro Val Tyr

70 75 80 85

ttg ggc gat cat gta cac cct ttg ggc gcc gtc ctg gag gct ttt gcg 403

Leu Gly Asp His Val His Pro Leu Gly Ala Val Leu Glu Ala Phe Ala

90 95 100

gaa aac gtg gtc act gcg ctg cgt gca ttt cag acg caa ttg gga gat 451

Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln Thr Gln Leu Gly Asp

105 110 115

acc tcc ccg atc gaa gta gtc att ggt gtg ccc gcc aac tcc cac agc 499

Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro Ala Asn Ser His Ser

120 125 130

gcc cag cga ctg ctc acc atg tcc gcc ttc agc gcc aca ggc atc acc 547

Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser Ala Thr Gly Ile Thr

135 140 145

gtt gtc ggt ttg gtc aat gag ccc agc gcc gca gct ttc gag tac acc 595

Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala Ala Phe Glu Tyr Thr

150 155 160 165

cac cgc cac gcc cgc acc tta aac tcc aag cgc caa gcc atc gtg gtt 643

His Arg His Ala Arg Thr Leu Asn Ser Lys Arg Gln Ala Ile Val Val

170 175 180

tat gat ttg gga ggc gga aca ttc gac tcc tcg ctc atc cgc atc gac 691

Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser Leu Ile Arg Ile Asp

185 190 195

ggc acc cac cac gag gtt gtg tcc tcc att ggc att tca cgc ctt ggt 739

Gly Thr His His Glu Val Val Ser Ser Ile Gly Ile Ser Arg Leu Gly

200 205 210

ggc gat gat ttc gat gaa atc ctc ctc caa tgc gcg ctc aag gcc gca 787

Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys Ala Leu Lys Ala Ala

215 220 225

ggc aga cag cac gat gcg ttt ggc aag cgt gct aaa aac acg ctt ctc 835

Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala Lys Asn Thr Leu Leu

230 235 240 245

gac gaa tcc cgc aac gcg aag gaa gct ctt gtt ccg caa tcc cgt cgc 883

Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val Pro Gln Ser Arg Arg

250 255 260

ttg gtt cta gaa att ggc gac gac gac atc acc gtt cca gtg aac aag 931

Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr Val Pro Val Asn Lys

265 270 275

ttc tac gag gct gcc act ccc ctg gtg gaa aaa tcc ttg tcc atc atg 979

Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys Ser Leu Ser Ile Met

280 285 290

gaa ccc ctc atc ggc gtc gat gat ctt aaa gat tcc gac atc gca ggc 1027

Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp Ser Asp Ile Ala Gly

295 300 305

atc tac ctt gtt ggt gga gga tcc tcg ctc cca ctc gtt tcc agg ttg 1075

Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro Leu Val Ser Arg Leu

310 315 320 325

ctc cgc gag cgt ttc ggc cgc cgt gtc cac cgc tcc cca ttc ccc tca 1123

Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg Ser Pro Phe Pro Ser

330 335 340

ggt tcc act gcg gtg ggt ctg gcc atc gcg gct gac cct tcc tct ggt 1171

Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala Asp Pro Ser Ser Gly

345 350 355

ttc cac cta agg gac cgc gtt gcg cga ggc atc ggt gtg ttc cgt gag 1219

Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile Gly Val Phe Arg Glu

360 365 370

cac gat tct ggt cgt gcc gtg agc ttt gac ccg ctg atc gcc ccg gac 1267

His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro Leu Ile Ala Pro Asp

375 380 385

acc gat tct gcg acc gtg gcg aaa cga tgc tac aag gcg gtg cac aac 1315

Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr Lys Ala Val His Asn

390 395 400 405

att ggt tgg ttc agg ttc gtg gaa tac tcc acc gtg tcc gag gat ggc 1363

Ile Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr Val Ser Glu Asp Gly

410 415 420

agc ccc gga gat att tcc ctg ctc agt gaa atc aag att cct ttt gat 1411

Ser Pro Gly Asp Ile Ser Leu Leu Ser Glu Ile Lys Ile Pro Phe Asp

425 430 435

agc tcc atc acc gat gtg gat gct acc gag att tca cgt ttc gat ggc 1459

Ser Ser Ile Thr Asp Val Asp Ala Thr Glu Ile Ser Arg Phe Asp Gly

440 445 450

cca gaa gta gaa gaa acc atc aca gtc aat gac aac ggc gtg gct tcc 1507

Pro Glu Val Glu Glu Thr Ile Thr Val Asn Asp Asn Gly Val Ala Ser

455 460 465

att tcc atc aag ata ctc ggc ggc gtt acc gtc gag cac aca att 1552

Ile Ser Ile Lys Ile Leu Gly Gly Val Thr Val Glu His Thr Ile

470 475 480

tagttaccat tttggtgctg gtggagtcca 1582

<210>30

<211>484

<212>PRT

<213>谷氨酸棒状杆菌

<400>30

Met Arg Phe Gly Leu Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala

1 5 10 15

Val Asp Arg Gly Asn Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly

20 25 30

Asp Thr His Asp Phe Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg

35 40 45

Ile Val Ala Gly Trp Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser

50 55 60

Phe Val Arg Ser Phe Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu

65 70 75 80

Ala Thr Pro Val Tyr Leu Gly Asp His Val His Pro Leu Gly Ala Val

85 90 95

Leu Glu Ala Phe Ala Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln

100 105 110

Thr Gln Leu Gly Asp Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro

115 120 125

Ala Asn Ser His Ser Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser

130 135 140

Ala Thr Gly Ile Thr Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala

145 150 155 160

Ala Phe Glu Tyr Thr His Arg His Ala Arg Thr Leu Asn Ser Lys Arg

165 170 175

Gln Ala Ile Val Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser

180 185 190

Leu Ile Arg Ile Asp Gly Thr His His Glu Val Val Ser Ser Ile Gly

195 200 205

Ile Ser Arg Leu Gly Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys

210 215 220

Ala Leu Lys Ala Ala Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala

225 230 235 240

Lys Asn Thr Leu Leu Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val

245 250 255

Pro Gln Ser Arg Arg Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr

260 265 270

Val Pro Val Asn Lys Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys

275 280 285

Ser Leu Ser Ile Met Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp

290 295 300

Ser Asp Ile Ala Gly Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro

305 310 315 320

Leu Val Ser Arg Leu Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg

325 330 335

Ser Pro Phe Pro Ser Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala

340 345 350

Asp Pro Ser Ser Gly Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile

355 360 365

Gly Val Phe Arg Glu His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro

370 375 380

Leu Ile Ala Pro Asp Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr

385 390 395 400

Lys Ala Val His Asn Ile Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr

405 410 415

Val Ser Glu Asp Gly Ser Pro Gly Asp Ile Ser Leu Leu Ser Glu Ile

420 425 430

Lys Ile Pro Phe Asp Ser Ser Ile Thr Asp Val Asp Ala Thr Glu Ile

435 440 445

Ser Arg Phe Asp Gly Pro Glu Val Glu Glu Thr Ile Thr Val Asn Asp

450 455 460

Asn Gly Val Ala Ser Ile Ser Ile Lys Ile Leu Gly Gly Val Thr Val

465 470 475 480

Glu His Thr Ile

<210>31

<211>1123

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1093)

<223>RXA01404

<220>

<221>unsure

<222>12..12

<223>所有n均表示任何核苷酸

<400>31

gtggatccgg tnttgtgatc cactacgcaa ttggagcgct ccaacacaag ctatatttgt 60

ttaaatgtcc tgtcaatagt tcaagagaaa atcacagaag atg agc aca tcc cgc 115

Met Ser Thr Ser Arg

1 5

ccc aca att tat gac gtc gcc aaa gcc gca ggc gtc tcc aaa tcc ttg 163

Pro Thr Ile Tyr Asp Val Ala Lys Ala Ala Gly Val Ser Lys Ser Leu

10 15 20

gtt tct ctc gtg ctt cgc ggc tcc ccc aac gtg agc aaa gaa tcc gaa 211

Val Ser Leu Val Leu Arg Gly Ser Pro Asn Val Ser Lys Glu Ser Glu

25 30 35

gcc gcg gtc aag acc gcg ata aaa aag ctc aac tac cag cca aat cgc 259

Ala Ala Val Lys Thr Ala Ile Lys Lys Leu Asn Tyr Gln Pro Asn Arg

40 45 50

gcc gca tca gac ctt gcg gcc aag cgc acg cag ctc att gca gtg ctt 307

Ala Ala Ser Asp Leu Ala Ala Lys Arg Thr Gln Leu Ile Ala Val Leu

55 60 65

atc gac gac tac tcc aac ccg tgg ttc atc gac ctg att caa agc ctc 355

Ile Asp Asp Tyr Ser Asn Pro Trp Phe Ile Asp Leu Ile Gln Ser Leu

70 75 80 85

agc gat gtg ctc acc ccc aag ggg tac cga ctg tcc gtc att gac tca 403

Ser Asp Val Leu Thr Pro Lys Gly Tyr Arg Leu Ser Val Ile Asp Ser

90 95 100

tta acc tct caa gcc ggc acc gat ccc att acc agt gca cta tca atg 451

Leu Thr Ser Gln Ala Gly Thr Asp Pro Ile Thr Ser Ala Leu Ser Met

105 110 115

cgc ccc gat gga atc atc atc gcc caa gac atc ccc gat ttc act gtc 499

Arg Pro Asp Gly Ile Ile Ile Ala Gln Asp Ile Pro Asp Phe Thr Val

120 125 130

ccc gat tcc cta ccc cca ttt gtc atc gca ggc acc aga atc acc caa 547

Pro Asp Ser Leu Pro Pro Phe Val Ile Ala Gly Thr Arg Ile Thr Gln

135 140 145

gcc agc acc cat gat tca gtg gcc aac gat gac ttc cgg ggc gca gaa 595

Ala Ser Thr His Asp Ser Val Ala Asn Asp Asp Phe Arg Gly Ala Glu

150 155 160 165

ata gcc aca aaa cac ctc atc gat ctt gga cac acc cac atc gcc cac 643

Ile Ala Thr Lys His Leu Ile Asp Leu Gly His Thr His Ile Ala His

170 175 180

cta cgc gtg gga agc ggc gct ggc tta cga cgc ttc gaa agc ttt gag 691

Leu Arg Val Gly Ser Gly Ala Gly Leu Arg Arg Phe Glu Ser Phe Glu

185 190 195

gca acc atg cgt gca cat ggc ctg gag ccg ctt tcc aac gat tac ctc 739

Ala Thr Met Arg Ala His Gly Leu Glu Pro Leu Ser Asn Asp Tyr Leu

200 205 210

ggc ccc gcc gtt gag cac gcc ggg tac acc gaa acc ctc gca cta ctc 787

Gly Pro Ala Val Glu His Ala Gly Tyr Thr Glu Thr Leu Ala Leu Leu

215 220 225

aaa gag cac ccg gag gtc acc gcc att ttc tcc tca aac gac atc acc 835

Lys Glu His Pro Glu Val Thr Ala Ile Phe Ser Ser Asn Asp Ile Thr

230 235 240 245

gcc atc gga gca ctc ggt gcc gcc cgt gaa cta ggt tta cgc gta cct 883

Ala Ile Gly Ala Leu Gly Ala Ala Arg Glu Leu Gly Leu Arg Val Pro

250 255 260

gaa gat cta tca ata atc gga tat gac aac act ccc ctc gcc caa acc 931

Glu Asp Leu Ser Ile Ile Gly Tyr Asp Asn Thr Pro Leu Ala Gln Thr

265 270 275

cga ctg atc aac ctc acc acc atc gac gac aac agc atc ggc gtc ggc 979

Arg Leu Ile Asn Leu Thr Thr Ile Asp Asp Asn Ser Ile Gly Val Gly

280 285 290

tac aac gcc gct ctc ttg ttg ctg agc atg ctt gat ccc gag gca ccc 1027

Tyr Asn Ala Ala Leu Leu Leu Leu Ser Met Leu Asp Pro Glu Ala Pro

295 300 305

cac ccg gag atc atg cat acg ttg cag ccc tcg ctg att gaa cgg ggc 1075

His Pro Glu Ile Met His Thr Leu Gln Pro Ser Leu Ile Glu Arg Gly

310 315 320 325

acg tgc gcg cca cgt gga tagctacccc aaatacttgg acttcctaat 1123

Thr Cys Ala Pro Arg Gly

330

<210>32

<211>331

<212>PRT

<213>谷氨酸棒状杆菌

<400>32

Met Ser Thr Ser Arg Pro Thr Ile Tyr Asp Val Ala Lys Ala Ala Gly

1 5 10 15

Val Ser Lys Ser Leu Val Ser Leu Val Leu Arg Gly Ser Pro Asn Val

20 25 30

Ser Lys Glu Ser Glu Ala Ala Val Lys Thr Ala Ile Lys Lys Leu Asn

35 40 45

Tyr Gln Pro Asn Arg Ala Ala Ser Asp Leu Ala Ala Lys Arg Thr Gln

50 55 60

Leu Ile Ala Val Leu Ile Asp Asp Tyr Ser Asn Pro Trp Phe Ile Asp

65 70 75 80

Leu Ile Gln Ser Leu Ser Asp Val Leu Thr Pro Lys Gly Tyr Arg Leu

85 90 95

Ser Val Ile Asp Ser Leu Thr Ser Gln Ala Gly Thr Asp Pro Ile Thr

100 105 110

Ser Ala Leu Ser Met Arg Pro Asp Gly Ile Ile Ile Ala Gln Asp Ile

115 120 125

Pro Asp Phe Thr Val Pro Asp Ser Leu Pro Pro Phe Val Ile Ala Gly

130 135 140

Thr Arg Ile Thr Gln Ala Ser Thr His Asp Ser Val Ala Asn Asp Asp

145 150 155 160

Phe Arg Gly Ala Glu Ile Ala Thr Lys His Leu Ile Asp Leu Gly His

165 170 175

Thr His Ile Ala His Leu Arg Val Gly Ser Gly Ala Gly Leu Arg Arg

180 185 190

Phe Glu Ser Phe Glu Ala Thr Met Arg Ala His Gly Leu Glu Pro Leu

195 200 205

Ser Asn Asp Tyr Leu Gly Pro Ala Val Glu His Ala Gly Tyr Thr Glu

210 215 220

Thr Leu Ala Leu Leu Lys Glu His Pro Glu Val Thr Ala Ile Phe Ser

225 230 235 240

Ser Asn Asp Ile Thr Ala Ile Gly Ala Leu Gly Ala Ala Arg Glu Leu

245 250 255

Gly Leu Arg Val Pro Glu Asp Leu Ser Ile Ile Gly Tyr Asp Asn Thr

260 265 270

Pro Leu Ala Gln Thr Arg Leu Ile Asn Leu Thr Thr Ile Asp Asp Asn

275 280 285

Ser Ile Gly Val Gly Tyr Asn Ala Ala Leu Leu Leu Leu Ser Met Leu

290 295 300

Asp Pro Glu Ala Pro His Pro Glu Ile Met His Thr Leu Gln Pro Ser

305 310 315 320

Leu Ile Glu Arg Gly Thr Cys Ala Pro Arg Gly

325 330

<210>33

<211>502

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(472)

<223>RXA01431

<400>33

caccgcagct ggttccggtt gccgcgcagc gatcgatgca gagcattacc tagcttctct 60

ggcctaattc acagttagcc ttaaaccaaa ccatgtacca atg aat gtc gga ttc 115

Met Asn Val Gly Phe

1 5

ccc agg agt ccc gtc att gtt aat tta gga gaa acc atg agc aat gtt 163

Pro Arg Ser Pro Val Ile Val Asn Leu Gly Glu Thr Met Ser Asn Val

10 15 20

gtt gca gta acc gag cag acc ttc aag tcc acc gtc atc gat tcc gac 211

Val Ala Val Thr Glu Gln Thr Phe Lys Ser Thr Val Ile Asp Ser Asp

25 30 35

aag cca gtc atc gtt gac ttc tgg gca gaa tgg tgt ggc ccc tgc aag 259

Lys Pro Val Ile Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Cys Lys

40 45 50

aag ctc agc ccc atc att gag gaa atc gca ggc gag tac ggc gac aag 307

Lys Leu Ser Pro Ile Ile Glu Glu Ile Ala Gly Glu Tyr Gly Asp Lys

55 60 65

gca gtc gtt gcc agc gtc gac gtc gat gca gag cgt acc ttg ggt gcc 355

Ala Val Val Ala Ser Val Asp Val Asp Ala Glu Arg Thr Leu Gly Ala

70 75 80 85

atg ttc cag att atg tcg att cct tct gtt ctc att ttc aaa aat ggt 403

Met Phe Gln Ile Met Ser Ile Pro Ser Val Leu Ile Phe Lys Asn Gly

90 95 100

gca aaa gtc gag gaa ttt gtc ggt ctg cgc ccc aag aac gaa att gtg 451

Ala Lys Val Glu Glu Phe Val Gly Leu Arg Pro Lys Asn Glu Ile Val

105 110 115

gaa aaa cta gag aag cac ctc tagctggtat tcttactgca gtcacgtgga 502

Glu Lys Leu Glu Lys His Leu

120

<210>34

<211>124

<212>PRT

<213>谷氨酸棒状杆菌

<400>34

Met Asn Val Gly Phe Pro Arg Ser Pro Val Ile Val Asn Leu Gly Glu

1 5 10 15

Thr Met Ser Asn Val Val Ala Val Thr Glu Gln Thr Phe Lys Ser Thr

20 25 30

Val Ile Asp Ser Asp Lys Pro Val Ile Val Asp Phe Trp Ala Glu Trp

35 40 45

Cys Gly Pro Cys Lys Lys Leu Ser Pro Ile Ile Glu Glu Ile Ala Gly

50 55 60

Glu Tyr Gly Asp Lys Ala Val Val Ala Ser Val Asp Val Asp Ala Glu

65 70 75 80

Arg Thr Leu Gly Ala Met Phe Gln Ile Met Ser Ile Pro Ser Val Leu

85 90 95

Ile Phe Lys Asn Gly Ala Lys Val Glu Glu Phe Val Gly Leu Arg Pro

100 105 110

Lys Asn Glu Ile Val Glu Lys Leu Glu Lys His Leu

115 120

<210>35

<211>1495

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1465)

<223>RXA01438

<400>35

ccattagcag tcgcaccccg ataggagtcg aatctacaag tggaaccccc gctcacatac 60

tccacatttt ttagaacccc tttaaggaat cgaactttat atg tct cgc cct ttg 115

Met Ser Arg Pro Leu

1 5

cgt gtt gcc gtt gtc ggt gca ggt cca gca gga atc tac gcg tct gat 163

Arg Val Ala Val Val Gly Ala Gly Pro Ala Gly Ile Tyr Ala Ser Asp

10 15 20

ttg ttg atg aaa tcc gac acg gac gtg cag att gat ctt ttt gaa cgt 211

Leu Leu Met Lys Ser Asp Thr Asp Val Gln Ile Asp Leu Phe Glu Arg

25 30 35

atg cca gcg cct ttc ggt ttg atc cgt tat ggt gtt gcg cct gat cac 259

Met Pro Ala Pro Phe Gly Leu Ile Arg Tyr Gly Val Ala Pro Asp His

40 45 50

cct cgc atc aag ggc atc gtg aag tcc ctg cac aat gtg atg gac aag 307

Pro Arg Ile Lys Gly Ile Val Lys Ser Leu His Asn Val Met Asp Lys

55 60 65

gag cag ctg cgt ttc ttg ggc aac att gag gtc ggc aag gac atc act 355

Glu Gln Leu Arg Phe Leu Gly Asn Ile Glu Val Gly Lys Asp Ile Thr

70 75 80 85

gtt gag gag ttg cgt gag ttt tat gac gcg atc gtg ttc tcc act ggc 403

Val Glu Glu Leu Arg Glu Phe Tyr Asp Ala Ile Val Phe Ser Thr Gly

90 95 100

gct act ggc gac cag gat ctt cgg gtt cca ggt tct gat ctg gaa ggt 451

Ala Thr Gly Asp Gln Asp Leu Arg Val Pro Gly Ser Asp Leu Glu Gly

105 110 115

tcg tgg ggc gct ggc gag ttc gtt ggt ttc tat gat ggc aac ccg aac 499

Ser Trp Gly Ala Gly Glu Phe Val Gly Phe Tyr Asp Gly Asn Pro Asn

120 125 130

ttt gaa cgc aac tgg gat ctt tct gct gag aag gta gcg gtt gtt ggt 547

Phe Glu Arg Asn Trp Asp Leu Ser Ala Glu Lys Val Ala Val Val Gly

135 140 145

gtc ggt aac gtg gcg ttg gac gtt gct cgt att ttg gcg aag act ggc 595

Val Gly Asn Val Ala Leu Asp Val Ala Arg Ile Leu Ala Lys Thr Gly

150 155 160 165

gat gag ctg cta gtt act gaa atc cct gac aat gtc tat gag agc ttg 643

Asp Glu Leu Leu Val Thr Glu Ile Pro Asp Asn Val Tyr Glu Ser Leu

170 175 180

gct aag aat cag gct aag gaa gtg cac gtt ttt ggt cgt cgt gga cct 691

Ala Lys Asn Gln Ala Lys Glu Val His Val Phe Gly Arg Arg Gly Pro

185 190 195

gct cag gcg aag ttc act ccg ttg gag ctg aag gaa ctt gac cat tcc 739

Ala Gln Ala Lys Phe Thr Pro Leu Glu Leu Lys Glu Leu Asp His Ser

200 205 210

gac acc atc gag gtg atc gtg aac cct gag gac att gat tac gat gca 787

Asp Thr Ile Glu Val Ile Val Asn Pro Glu Asp Ile Asp Tyr Asp Ala

215 220 225

gct tcg gag cag gct cgt cgt gat tcc aag tct cag gac ctc gtg tgc 835

Ala Ser Glu Gln Ala Arg Arg Asp Ser Lys Ser Gln Asp Leu Val Cys

230 235 240 245

cag act ttg gaa agc tac gcg atg cgc gat cct aag ggc gct cct cac 883

Gln Thr Leu Glu Ser Tyr Ala Met Arg Asp Pro Lys Gly Ala Pro His

250 255 260

aag ctg ttc att cac ttc ttt gag tcc cca gtg gag atc ctc ggt gag 931

Lys Leu Phe Ile His Phe Phe Glu Ser Pro Val Glu Ile Leu Gly Glu

265 270 275

gac ggc aag gtt gtt ggc ctc aag act gag cgt act cag ctg gac ggc 979

Asp Gly Lys Val Val Gly Leu Lys Thr Glu Arg Thr Gln Leu Asp Gly

280 285 290

aac ggt ggc gtg act ggc acc ggc gag ttc aag acc tgg gat atg cag 1027

Asn Gly Gly Val Thr Gly Thr Gly Glu Phe Lys Thr Trp Asp Met Gln

295 300 305

tca gtt tac cgc gcg gta ggt tac cgt tct gat gcg atc gag ggt gtt 1075

Ser Val Tyr Arg Ala Val Gly Tyr Arg Ser Asp Ala Ile Glu Gly Val

310 315 320 325

cct ttt gac gat gag cgc gcg gtt gtc ccc aac gac ggc ggc cac atc 1123

Pro Phe Asp Asp Glu Arg Ala Val Val Pro Asn Asp Gly Gly His Ile

330 335 340

atc gat cct gag gtc ggc tcc ccc atc act ggc ctg tac gcc act ggc 1171

Ile Asp Pro Glu Val Gly Ser Pro Ile Thr Gly Leu Tyr Ala Thr Gly

345 350 355

tgg atc aag cgt ggc cca att gga ctg atc ggc aac acc aag tcc gac 1219

Trp Ile Lys Arg Gly Pro Ile Gly Leu Ile Gly Asn Thr Lys Ser Asp

360 365 370

gcc aag gaa acc act gag atg ctg ctt gct gat cac gct gct ggt tct 1267

Ala Lys Glu Thr Thr Glu Met Leu Leu Ala Asp His Ala Ala Gly Ser

375 380 385

ttg cct gcg cct gca aag cct gag ttg gag tcc atc att gag ttc ctc 1315

Leu Pro Ala Pro Ala Lys Pro Glu Leu Glu Ser Ile Ile Glu Phe Leu

390 395 400 405

gat gag cgc aag gtt gcg ttc acc aca tgg gat ggc tgg cac ctg ctg 1363

Asp Glu Arg Lys Val Ala Phe Thr Thr Trp Asp Gly Trp His Leu Leu

410 415 420

gat gct gcg gag cgc gcg ctg ggt gag cct gag ggc cgc gag cgc aag 1411

Asp Ala Ala Glu Arg Ala Leu Gly Glu Pro Glu Gly Arg Glu Arg Lys

425 430 435

aag atc gtt gag tgg aat gac atg gtg cgc cat gct cgt cca gaa tac 1459

Lys Ile Val Glu Trp Asn Asp Met Val Arg His Ala Arg Pro Glu Tyr

440 445 450

gac atc taaagtcgct taaagcctca aaaaagggcg 1495

Asp Ile

455

<210>36

<211>455

<212>PRT

<213>谷氨酸棒状杆菌

<400>36

Met Ser Arg Pro Leu Arg Val Ala Val Val Gly Ala Gly Pro Ala Gly

1 5 10 15

Ile Tyr Ala Ser Asp Leu Leu Met Lys Ser Asp Thr Asp Val Gln Ile

20 25 30

Asp Leu Phe Glu Arg Met Pro Ala Pro Phe Gly Leu Ile Arg Tyr Gly

35 40 45

Val Ala Pro Asp His Pro Arg Ile Lys Gly Ile Val Lys Ser Leu His

50 55 60

Asn Val Met Asp Lys Glu Gln Leu Arg Phe Leu Gly Asn Ile Glu Val

65 70 75 80

Gly Lys Asp Ile Thr Val Glu Glu Leu Arg Glu Phe Tyr Asp Ala Ile

85 90 95

Val Phe Ser Thr Gly Ala Thr Gly Asp Gln Asp Leu Arg Val Pro Gly

100 105 110

Ser Asp Leu Glu Gly Ser Trp Gly Ala Gly Glu Phe Val Gly Phe Tyr

115 120 125

Asp Gly Asn Pro Asn Phe Glu Arg Asn Trp Asp Leu Ser Ala Glu Lys

130 135 140

Val Ala Val Val Gly Val Gly Asn Val Ala Leu Asp Val Ala Arg Ile

145 150 155 160

Leu Ala Lys Thr Gly Asp Glu Leu Leu Val Thr Glu Ile Pro Asp Asn

165 170 175

Val Tyr Glu Ser Leu Ala Lys Asn Gln Ala Lys Glu Val His Val Phe

180 185 190

Gly Arg Arg Gly Pro Ala Gln Ala Lys Phe Thr Pro Leu Glu Leu Lys

195 200 205

Glu Leu Asp His Ser Asp Thr Ile Glu Val Ile Val Asn Pro Glu Asp

210 215 220

Ile Asp Tyr Asp Ala Ala Ser Glu Gln Ala Arg Arg Asp Ser Lys Ser

225 230 235 240

Gln Asp Leu Val Cys Gln Thr Leu Glu Ser Tyr Ala Met Arg Asp Pro

245 250 255

Lys Gly Ala Pro His Lys Leu Phe Ile His Phe Phe Glu Ser Pro Val

260 265 270

Glu Ile Leu Gly Glu Asp Gly Lys Val Val Gly Leu Lys Thr Glu Arg

275 280 285

Thr Gln Leu Asp Gly Asn Gly Gly Val Thr Gly Thr Gly Glu Phe Lys

290 295 300

Thr Trp Asp Met Gln Ser Val Tyr Arg Ala Val Gly Tyr Arg Ser Asp

305 310 315 320

Ala Ile Glu Gly Val Pro Phe Asp Asp Glu Arg Ala Val Val Pro Asn

325 330 335

Asp Gly Gly His Ile Ile Asp Pro Glu Val Gly Ser Pro Ile Thr Gly

340 345 350

Leu Tyr Ala Thr Gly Trp Ile Lys Arg Gly Pro Ile Gly Leu Ile Gly

355 360 365

Asn Thr Lys Ser Asp Ala Lys Glu Thr Thr Glu Met Leu Leu Ala Asp

370 375 380

His Ala Ala Gly Ser Leu Pro Ala Pro Ala Lys Pro Glu Leu Glu Ser

385 390 395 400

Ile Ile Glu Phe Leu Asp Glu Arg Lys Val Ala Phe Thr Thr Trp Asp

405 410 415

Gly Trp His Leu Leu Asp Ala Ala Glu Arg Ala Leu Gly Glu Pro Glu

420 425 430

Gly Arg Glu Arg Lys Lys Ile Val Glu Trp Asn Asp Met Val Arg His

435 440 445

Ala Arg Pro Glu Tyr Asp Ile

450 455

<210>37

<211>1021

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(991)

<223>RXA01490

<400>37

cacaccaatg gtgactactg atccttgaag atcagccgga acgctgtcta gtccactcca 60

aatatccact gttttagact acggcataga ctcaacagac atg aat gct cct gcc 115

Met Asn Ala Pro Ala

1 5

cct aaa cct gga ctc gtg atc gtc gac aag ccc gcc gga atg aca tcc 163

Pro Lys Pro Gly Leu Val Ile Val Asp Lys Pro Ala Gly Met Thr Ser

10 15 20

cat gac gtg gtg tcc aaa ttg cgc cgc gca ttt tcc acc cgc aaa gta 211

His Asp Val Val Ser Lys Leu Arg Arg Ala Phe Ser Thr Arg Lys Val

25 30 35

ggc cac gca ggc acc ctc gac ccc atg gca acc ggc gtg tta gtc gtc 259

Gly His Ala Gly Thr Leu Asp Pro Met Ala Thr Gly Val Leu Val Val

40 45 50

gga att gag cgc gga acc cgc ttc ctg gca cac atg gtg gcc tcc acc 307

Gly Ile Glu Arg Gly Thr Arg Phe Leu Ala His Met Val Ala Ser Thr

55 60 65

aaa gcc tac gac gcc acc att cga ctc ggc gcc gcc acc agc acc gat 355

Lys Ala Tyr Asp Ala Thr Ile Arg Leu Gly Ala Ala Thr Ser Thr Asp

70 75 80 85

gat gca gaa ggc gag gtt atc tcc aca aca gac gca tcc ggc ctc gac 403

Asp Ala Glu Gly Glu Val Ile Ser Thr Thr Asp Ala Ser Gly Leu Asp

90 95 100

cac agc acc atc ctt gct gaa atc gtc aac ctc acc ggc gac atc atg 451

His Ser Thr Ile Leu Ala Glu Ile Val Asn Leu Thr Gly Asp Ile Met

105 110 115

caa aaa ccc acc aaa gtc tcc gcc atc aaa atc gac ggc aaa cgc gcc 499

Gln Lys Pro Thr Lys Val Ser Ala Ile Lys Ile Asp Gly Lys Arg Ala

120 125 130

cac gaa cgc gtc cgc gac ggc gaa gaa gta gac att ccc gca cgt ccc 547

His Glu Arg Val Arg Asp Gly Glu Glu Val Asp Ile Pro Ala Arg Pro

135 140 145

gtc acc gtc agc gtc ttt gac gtg ctc gac tac cac gtc gac ggt gaa 595

Val Thr Val Ser Val Phe Asp Val Leu Asp Tyr His Val Asp Gly Glu

150 155 160 165

ttt tat gac tta gat gtg cgc gtc cac tgc tcc tcc ggc acc tac atc 643

Phe Tyr Asp Leu Asp Val Arg Val His Cys Ser Ser Gly Thr Tyr Ile

170 175 180

cgc gcg ctc gcc cgc gac ctc ggc aac gct ttg cag gtc ggc ggc cac 691

Arg Ala Leu Ala Arg Asp Leu Gly Asn Ala Leu Gln Val Gly Gly His

185 190 195

ctg acc gcg ctt agg cgc aca gag gtc ggc cct ttt acg ctt aac gac 739

Leu Thr Ala Leu Arg Arg Thr Glu Val Gly Pro Phe Thr Leu Asn Asp

200 205 210

gcg acc ccc ctc tcc aaa ctc caa gag aat cca gaa ctc tcc ctc aac 787

Ala Thr Pro Leu Ser Lys Leu Gln Glu Asn Pro Glu Leu Ser Leu Asn

215 220 225

ctc gac cag gca ctc acc cgc agt tac cca gtc ctt gac atc acc gaa 835

Leu Asp Gln Ala Leu Thr Arg Ser Tyr Pro Val Leu Asp Ile Thr Glu

230 235 240 245

gac gaa ggc gtt gac ctg tcc atg ggc aaa tgg ttg gaa cct cgc gga 883

Asp Glu Gly Val Asp Leu Ser Met Gly Lys Trp Leu Glu Pro Arg Gly

250 255 260

ctg aaa ggc gtc cac gct gca gta aca cca tca gga aaa gcc gtg gcg 931

Leu Lys Gly Val His Ala Ala Val Thr Pro Ser Gly Lys Ala Val Ala

265 270 275

ctc atc gaa gaa aag ggc aaa cgc ctg gcc acc gtg ttt gtt gct cac 979

Leu Ile Glu Glu Lys Gly Lys Arg Leu Ala Thr Val Phe Val Ala His

280 285 290

ccc aac act ctt tagttggtct gccagaagcc gatttaagag 1021

Pro Asn Thr Leu

295

<210>38

<211>297

<212>PRT

<213>谷氨酸棒状杆菌

<400>38

Met Asn Ala Pro Ala Pro Lys Pro Gly Leu Val Ile Val Asp Lys Pro

1 5 10 15

Ala Gly Met Thr Ser His Asp Val Val Ser Lys Leu Arg Arg Ala Phe

20 25 30

Ser Thr Arg Lys Val Gly His Ala Gly Thr Leu Asp Pro Met Ala Thr

35 40 45

Gly Val Leu Val Val Gly Ile Glu Arg Gly Thr Arg Phe Leu Ala His

50 55 60

Met Val Ala Ser Thr Lys Ala Tyr Asp Ala Thr Ile Arg Leu Gly Ala

65 70 75 80

Ala Thr Ser Thr Asp Asp Ala Glu Gly Glu Val Ile Ser Thr Thr Asp

85 90 95

Ala Ser Gly Leu Asp His Ser Thr Ile Leu Ala Glu Ile Val Asn Leu

100 105 110

Thr Gly Asp Ile Met Gln Lys Pro Thr Lys Val Ser Ala Ile Lys Ile

115 120 125

Asp Gly Lys Arg Ala His Glu Arg Val Arg Asp Gly Glu Glu Val Asp

130 135 140

Ile Pro Ala Arg Pro Val Thr Val Ser Val Phe Asp Val Leu Asp Tyr

145 150 155 160

His Val Asp Gly Glu Phe Tyr Asp Leu Asp Val Arg Val His Cys Ser

165 170 175

Ser Gly Thr Tyr Ile Arg Ala Leu Ala Arg Asp Leu Gly Asn Ala Leu

180 185 190

Gln Val Gly Gly His Leu Thr Ala Leu Arg Arg Thr Glu Val Gly Pro

195 200 205

Phe Thr Leu Asn Asp Ala Thr Pro Leu Ser Lys Leu Gln Glu Asn Pro

210 215 220

Glu Leu Ser Leu Asn Leu Asp Gln Ala Leu Thr Arg Ser Tyr Pro Val

225 230 235 240

Leu Asp Ile Thr Glu Asp Glu Gly Val Asp Leu Ser Met Gly Lys Trp

245 250 255

Leu Glu Pro Arg Gly Leu Lys Gly Val His Ala Ala Val Thr Pro Ser

260 265 270

Gly Lys Ala Val Ala Leu Ile Glu Glu Lys Gly Lys Arg Leu Ala Thr

275 280 285

Val Phe Val Ala His Pro Asn Thr Leu

290 295

<210>39

<211>1441

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1411)

<223>RXA01493

<400>39

cctgctgcag gctataccgc tcgtggtacg gaaatcgaag ccctcgatac gttgattgaa 60

gcaaccgtta ccttggggga gtctttgcga agctcggcgc atg tcg atg tct aac 115

Met Ser Met Ser Asn

1 5

aac gac ttt gag cat gag tcc cat gat gtt tct gca aag cag atc ttc 163

Asn Asp Phe Glu His Glu Ser His Asp Val Ser Ala Lys Gln Ile Phe

10 15 20

ggg ctc gcg ttc ccc gca ctg ggt gtt cta gct gcg atg ccg ctg tat 211

Gly Leu Ala Phe Pro Ala Leu Gly Val Lau Ala Ala Met Pro Leu Tyr

25 30 35

ctc ttg ttg gat aca gcg gtt gtt ggc act ttg ggt ggc ttc gaa ttg 259

Leu Leu Leu Asp Thr Ala Val Val Gly Thr Leu Gly Gly Phe Glu Leu

40 45 50

gct gcg ttg ggc gca gca aca aca att caa gct caa gtg aca aca cag 307

Ala Ala Leu Gly Ala Ala Thr Thr Ile Gln Ala Gln Val Thr Thr Gln

55 60 65

ctg aca ttc ttg tcc tat gga act acc gcg aga tca tcg aga att ttc 355

Leu Thr Phe Leu Ser Tyr Gly Thr Thr Ala Arg Ser Ser Arg Ile Phe

70 75 80 85

gga atg ggt gat cgc cgg gga gca att gcc gaa ggt gtg caa gca acc 403

Gly Met Gly Asp Arg Arg Gly Ala Ile Ala Glu Gly Val Gln Ala Thr

90 95 100

tgg gtg gca ctc ttt gta ggc ttg ggc atc tta acg ctg atg ctc att 451

Trp Val Ala Leu Phe Val Gly Leu Gly Ile Leu Thr Leu Met Leu Ile

105 110 115

gga gcc ccg act ttc gcg ttg tgg ctc agt ggt gat gaa gct cta gcc 499

Gly Ala Pro Thr Phe Ala Leu Trp Leu Ser Gly Asp Glu Ala Leu Ala

120 125 130

caa gaa gca ggg cat tgg ctc cgg gtc gct gct ttt gcg gtg cca cta 547

Gln Glu Ala Gly His Trp Leu Arg Val Ala Ala Phe Ala Val Pro Leu

135 140 145

att ctc atg atc atg gct ggc aac ggt tgg tta aga ggt att caa aac 595

Ile Leu Met Ile Met Ala Gly Asn Gly Trp Leu Arg Gly Ile Gln Asn

150 155 160 165

acc aag ctg cca ctc tat ttc acc ttg gcg gga gtc atc ccc ggc gcg 643

Thr Lys Leu Pro Leu Tyr Phe Thr Leu Ala Gly Val Ile Pro Gly Ala

170 175 180

atc ttg att ccg ata ttc gtg gct aag ttt gga ctt gtg ggc tct gcc 691

Ile Leu Ile Pro Ile Phe Val Ala Lys Phe Gly Leu Val Gly Ser Ala

185 190 195

tgg gca aac ctc att gca gaa gca att act gct tcg ctg ttt ttg ggt 739

Trp Ala Asn Leu Ile Ala Glu Ala Ile Thr Ala Ser Leu Phe Leu Gly

200 205 210

gca ttg atc aag cac cac gaa ggt tcg tgg aag ccg agc tgg acg gtg 787

Ala Leu Ile Lys His His Glu Gly Ser Trp Lys Pro Ser Trp Thr Val

215 220 225

atg aaa aat cag ttg gtt ctt gga cgt gat ttg atc atg cgg tca atg 835

Met Lys Asn Gln Leu Val Leu Gly Arg Asp Leu Ile Met Arg Ser Met

230 235 240 245

tcg ttc cag gtt gct ttt ctt tcc gcg gcc gct gtg gct gca cga ttt 883

Ser Phe Gln Val Ala Phe Leu Ser Ala Ala Ala Val Ala Ala Arg Phe

250 255 260

ggc acg gca tcc ttg gcg gcc cac cag gtg ttg ctt cag ctg tgg aat 931

Gly Thr Ala Ser Leu Ala Ala His Gln Val Leu Leu Gln Leu Trp Asn

265 270 275

ttc atc aca ttg gtg ctg gat tct cta gct atc gcg gcg cag acc tta 979

Phe Ile Thr Leu Val Leu Asp Ser Leu Ala Ile Ala Ala Gln Thr Leu

280 285 290

act ggt gca gcc ctg ggc gct gga act gcg aag gtc gcc cgc agg gtg 1027

Thr Gly Ala Ala Leu Gly Ala Gly Thr Ala Lys Val Ala Arg Arg Val

295 300 305

ggt aat cag gtg att aag tac tct ctg att ttc gct ggt ggc tta ggt 1075

Gly Asn Gln Val Ile Lys Tyr Ser Leu Ile Phe Ala Gly Gly Leu Gly

310 315 320 325

ttg gtg ttc gtg gtc tta cac tcg tgg att ccg cgt att ttc act cag 1123

Leu Val Phe Val Val Leu His Ser Trp Ile Pro Arg Ile Phe Thr Gln

330 335 340

gac gcc gac gtt tta gat gcg att gct tcc ccg tgg tgg atc atg gtc 1171

Asp Ala Asp Val Leu Asp Ala Ile Ala Ser Pro Trp Trp Ile Met Val

345 350 355

gcg atg atc att ttg ggt ggc att gtc ttt gct att gat ggt gtg ctg 1219

Ala Met Ile Ile Leu Gly Gly Ile Val Phe Ala Ile Asp Gly Val Leu

360 365 370

ttg ggt gct gct gac gcg gtg ttc ctc cga aat gcc tct atc ttg gcg 1267

Leu Gly Ala Ala Asp Ala Val Phe Leu Arg Asn Ala Ser Ile Leu Ala

375 380 385

gtt gtg gtc gga ttc tta cca ggc gtc tgg att tcc tat gca tta gat 1315

Val Val Val Gly Phe Leu Pro Gly Val Trp Ile Ser Tyr Ala Leu Agp

390 395 400 405

gca ggg ctg aca ggc gtg tgg tgt ggt ttg ctg gcg ttt att ctg atc 1363

Ala Gly Leu Thr Gly Val Trp Cys Gly Leu Leu Ala Phe Ile Leu Ile

410 415 420

cga cta ttt gcg gtg att tgg cgg ttt aag tct atg aag tgg gcg cgt 1411

Arg Leu Phe Ala Val Ile Trp Arg Phe Lys Ser Met Lys Trp Ala Arg

425 430 435

tagcttcggc gcgtggcaaa ccacatttgc 1441

<210>40

<211>437

<212>PRT

<213>谷氨酸棒状杆菌

<400>40

Met Ser Met Ser Asn Asn Asp Phe Glu His Glu Ser His Asp Val Ser

1 5 10 15

Ala Lys Gln Ile Phe Gly Leu Ala Phe Pro Ala Leu Gly Val Leu Ala

20 25 30

Ala Met Pro Leu Tyr Leu Leu Leu Asp Thr Ala Val Val Gly Thr Leu

35 40 45

Gly Gly Phe Glu Leu Ala Ala Leu Gly Ala Ala Thr Thr Ile Gln Ala

50 55 60

Gln Val Thr Thr Gln Leu Thr Phe Leu Ser Tyr Gly Thr Thr Ala Arg

65 70 75 80

Ser Ser Arg Ile Phe Gly Met Gly Asp Arg Arg Gly Ala Ile Ala Glu

85 90 95

Gly Val Gln Ala Thr Trp Val Ala Leu Phe Val Gly Leu Gly Ile Leu

100 105 110

Thr Leu Met Leu Ile Gly Ala Pro Thr Phe Ala Leu Trp Leu Ser Gly

115 120 125

Asp Glu Ala Leu Ala Gln Glu Ala Gly His Trp Leu Arg Val Ala Ala

130 135 140

Phe Ala Val Pro Leu Ile Leu Met Ile Met Ala Gly Asn Gly Trp Leu

145 150 155 160

Arg Gly Ile Gln Asn Thr Lys Leu Pro Leu Tyr Phe Thr Leu Ala Gly

165 170 175

Val Ile Pro Gly Ala Ile Leu Ile Pro Ile Phe Val Ala Lys Phe Gly

180 185 190

Leu Val Gly Ser Ala Trp Ala Asn Leu Ile Ala Glu Ala Ile Thr Ala

195 200 205

Ser Leu Phe Leu Gly Ala Leu Ile Lys His His Glu Gly Ser Trp Lys

210 215 220

Pro Ser Trp Thr Val Met Lys Asn Gln Leu Val Leu Gly Arg Asp Leu

225 230 235 240

Ile Met Arg Ser Met Ser Phe Gln Val Ala Phe Leu Ser Ala Ala Ala

245 250 255

Val Ala Ala Arg Phe Gly Thr Ala Ser Leu Ala Ala His Gln Val Leu

260 265 270

Leu Gln Leu Trp Asn Phe Ile Thr Leu Val Leu Asp Ser Leu Ala Ile

275 280 285

Ala Ala Gln Thr Leu Thr Gly Ala Ala Leu Gly Ala Gly Thr Ala Lys

290 295 300

Val Ala Arg Arg Val Gly Asn Gln Val Ile Lys Tyr Ser Leu Ile Phe

305 310 315 320

Ala Gly Gly Leu Gly Leu Val Phe Val Val Leu His Ser Trp Ile Pro

325 330 335

Arg Ile Phe Thr Gln Asp Ala Asp Val Leu Asp Ala Ile Ala Ser Pro

340 345 350

Trp Trp Ile Met Val Ala Met Ile Ile Leu Gly Gly Ile Val Phe Ala

355 360 365

Ile Asp Gly Val Leu Leu Gly Ala Ala Asp Ala Val Phe Leu Arg Asn

370 375 380

Ala Ser Ile Leu Ala Val Val Val Gly Phe Leu Pro Gly Val Trp Ile

385 390 395 400

Ser Tyr Ala Leu Asp Ala Gly Leu Thr Gly Val Trp Cys Gly Leu Leu

405 410 415

Ala Phe Ile Leu Ile Arg Leu Phe Ala Val Ile Trp Arg Phe Lys Ser

420 425 430

Met Lys Trp Ala Arg

435

<210>41

<211>2056

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2026)

<223>RXA01559

<400>41

ctttctcgct cgtgtcgcac tcacccacgc cacctggcgt gggtgagtgg cgcatggagt 60

gggtgggcgt cgacaagcgt ggttgtctgg ttgattggaa ttg aag gag act ttc 115

Leu Lys Glu Thr Phe

1 5

ttg gct cgg caa aaa aag agt gcc gct agc gcc tgg gaa cga tgg cca 163

Leu Ala Arg Gln Lys Lys Ser Ala Ala Ser Ala Trp Glu Arg Trp Pro

10 15 20

aaa cgc gca ata gcg ttg ttt gtg ctc atc gtc gtt ggt gtt tat gcg 211

Lys Arg Ala Ile Ala Leu Phe Val Leu Ile Val Val Gly Val Tyr Ala

25 30 35

ttg gtg ctg ttg aca ggc gat cgt tct gcc aca cca aaa ttg ggt att 259

Leu Val Leu Leu Thr Gly Asp Arg Ser Ala Thr Pro Lys Leu Gly Ile

40 45 50

gat ctg caa ggc gga acc cga gtg acc ctc gtg ccg cag ggg cag gat 307

Asp Leu Gln Gly Gly Thr Arg Val Thr Leu Val Pro Gln Gly Gln Asp

55 60 65

cca act cag gac cag ctg aat cag gca cgc acc att ctg gaa aac cgt 355

Pro Thr Gln Asp Gln Leu Asn Gln Ala Arg Thr Ile Leu Glu Asn Arg

70 75 80 85

gtg aac ggc atg ggc gtt tca ggt gca agc gtg gtc gct gac ggt aac 403

Val Asn Gly Met Gly Val Ser Gly Ala Ser Val Val Ala Asp Gly Asn

90 95 100

acg ctg gtg atc act gtt ccc ggg gaa aat acc gca cag gcg caa tcc 451

Thr Leu Val Ile Thr Val Pro Gly Glu Asn Thr Ala Gln Ala Gln Ser

105 110 115

cta gga cag acc tcc cag ctg ctg ttc cgt ccc gtt ggt cag gca gga 499

Leu Gly Gln Thr Ser Gln Leu Leu Phe Arg Pro Val Gly Gln Ala Gly

120 125 130

atg ccc gat atg acc acg ttg atg cca gag ctg gaa gag atg gcc aac 547

Met Pro Asp Met Thr Thr Leu Met Pro Glu Leu Glu Glu Met Ala Asn

135 140 145

agg tgg gtt gaa tac ggc gtc atc acc gaa gag cag gca aat gcc tcc 595

Arg Trp Val Glu Tyr Gly Val Ile Thr Glu Glu Gln Ala Asn Ala Ser

150 155 160 165

ttg gag gaa atg aac acc gct gtt gca tcg acc act gcg gtg gaa ggc 643

Leu Glu Glu Met Asn Thr Ala Val Ala Ser Thr Thr Ala Val Glu Gly

170 175 180

gaa gaa gca act gag cca gaa ccc gtc acc gtg tcg gcg acc cct atg 691

Glu Glu Ala Thr Glu Pro Glu Pro Val Thr Val Ser Ala Thr Pro Met

185 190 195

gat gag cca gcc aac tcc att gag gca aca cag cga cgc cag gaa atc 739

Asp Glu Pro Ala Asn Ser Ile Glu Ala Thr Gln Arg Arg Gln Glu Ile

200 205 210

acg gac atg ctg cgc acc gac cgc cag tcc acc gat ccc act gtc cag 787

Thr Asp Met Leu Arg Thr Asp Arg Gln Ser Thr Asp Pro Thr Val Gln

215 220 225

atc gct gca agt tct ttg atg cag tgc acc act gat gag atg gat cct 835

Ile Ala Ala Ser Ser Leu Met Gln Cys Thr Thr Asp Glu Met Asp Pro

230 235 240 245

ttg gcc ggc acc gat gat cca cgc ctg cca ttg gtg gca tgt gat cca 883

Leu Ala Gly Thr Asp Asp Pro Arg Leu Pro Leu Val Ala Cys Asp Pro

250 255 260

gct gta ggt ggc gtg tat gta ctt gat cct gca cct ttg ctc aac ggc 931

Ala Val Gly Gly Val Tyr Val Leu Asp Pro Ala Pro Leu Leu Asn Gly

265 270 275

gaa acc gat gag gaa aat ggt gcg cgc cta acc ggt aat gag atc gat 979

Glu Thr Asp Glu Glu Asn Gly Ala Arg Leu Thr Gly Asn Glu Ile Asp

280 285 290

acc aac cgt ccc atc acc ggt gga ttc aac gcc cag tcc ggc cag atg 1027

Thr Asn Arg Pro Ile Thr Gly Gly Phe Asn Ala Gln Ser Gly Gln Met

295 300 305

gaa atc agc ttt gcc ttc aaa tcc ggc gat ggg gaa gaa ggc tct gca 1075

Glu Ile Ser Phe Ala Phe Lys Ser Gly Asp Gly Glu Glu Gly Ser Ala

310 315 320 325

act tgg tcc tct ctg acc agc cag tac ctg cag cag cag atc gcc atc 1123

Thr Trp Ser Ser Leu Thr Ser Gln Tyr Leu Gln Gln Gln Ile Ala Ile

330 335 340

acc ctg gac tct cag gtg att tct gca ccc gtg att cag tca gca acc 1171

Thr Leu Asp Ser Gln Val Ile Ser Ala Pro Val Ile Gln Ser Ala Thr

345 350 355

cct gtg ggt tct gca aca tcc atc acc ggt gac ttc act caa act gaa 1219

Pro Val Gly Ser Ala Thr Ser Ile Thr Gly Asp Phe Thr Gln Thr Glu

360 365 370

gcc caa gat ctg gcg aac aac ctg cgc tac ggt gca ttg ccc ctg agc 1267

Ala Gln Asp Leu Ala Asn Asn Leu Arg Tyr Gly Ala Leu Pro Leu Ser

375 380 385

ttc gca ggt gaa aac ggc gag cgc ggc gga act acc acc acc gtt ccg 1315

Phe Ala Gly Glu Asn Gly Glu Arg Gly Gly Thr Thr Thr Thr Val Pro

390 395 400 405

cca tca cta ggc gca gca tcc ttg aag gcc gga ctg atc gca ggc atc 1363

Pro Ser Leu Gly Ala Ala Ser Leu Lys Ala Gly Leu Ile Ala Gly Ile

410 415 420

gtc ggc atc gcg ctg gtc gcc atc ttc gtg ttc gcc tac tac cgc gtc 1411

Val Gly Ile Ala Leu Val Ala Ile Phe Val Phe Ala Tyr Tyr Arg Val

425 430 435

ttc gga ttc gtt tcc ctg ttc acc ctg ttt gcc gca ggc gtg ttg gtc 1459

Phe Gly Phe Val Ser Leu Phe Thr Leu Phe Ala Ala Gly Val Leu Val

440 445 450

tac ggc ctt ctg gta ctg ctg gga cgc tgg atc gga tat tcc cta gac 1507

Tyr Gly Leu Leu Val Leu Leu Gly Arg Trp Ile Gly Tyr Ser Leu Asp

455 460 465

ctt gct ggt atc gcc ggt ttg atc atc ggt atc ggt acc acc gcc gac 1555

Leu Ala Gly Ile Ala Gly Leu Ile Ile Gly Ile Gly Thr Thr Ala Asp

470 475 480 485

tcc ttc gtg gtg ttc tat gag cgc atc aag gat gag atc cgt gaa gga 1603

Ser Phe Val Val Phe Tyr Glu Arg Ile Lys Asp Glu Ile Arg Glu Gly

490 495 500

aga tcc ttt aga tct gca gta cct cgt gca tgg gaa agc gcc aag cgc 1651

Arg Ser Phe Arg Ser Ala Val Pro Arg Ala Trp Glu Ser Ala Lys Arg

505 510 515

acc atc gtc aca ggc aac atg gtc act ttg ctc ggc gct atc gtg att 1699

Thr Ile Val Thr Gly Asn Met Val Thr Leu Leu Gly Ala Ile Val Ile

520 525 530

tac ttg ctc gcg gtc ggc gaa gtc aag ggc ttt gcc ttc acc ctg ggt 1747

Tyr Leu Leu Ala Val Gly Glu Val nys Gly Phe Ala Phe Thr Leu Gly

535 540 545

ctg acc acc gta ttc gat ctc gtt gtc acc ttc ctg atc acg gca cca 1795

Leu Thr Thr Val Phe Asp Leu Val Val Thr Phe Leu Ile Thr Ala Pro

550 555 560 565

ctg gtt atc ctg gca tca cgc aac cca ttc ttt gcc aag tca tcg gtc 1843

Leu Val Ile Leu Ala Ser Arg Asn Pro Phe Phe Ala Lys Ser Ser Val

570 575 580

aac ggc atg gga cga gtg atg aag ctc gtt gaa gaa cgc cgc gcc aac 1891

Asn Gly Met Gly Arg Val Met Lys Leu Val Glu Glu Arg Arg Ala Asn

585 590 595

ggt gaa ttg gat gag cct gag tac ctg aaa aag atc cat gcc aag aat 1939

Gly Glu Leu Asp Glu Pro Glu Tyr Leu Lys Lys Ile His Ala Lys Asn

600 605 610

gcg gca gct gat aag gct tcc act gac aat tct tcc act gac aat tct 1987

Ala Ala Ala Asp Lys Ala Ser Thr Asp Asn Ser Ser Thr Asp Asn Ser

615 620 625

gaa gca cct ggc acc gat acg aac caa gag gag gag aag tagccatgac 2036

Glu Ala Pro Gly Thr Asp Thr Asn Gln Glu Glu Glu Lys

630 635 640

tgattcccag actgaatcac 2056

<210>42

<211>642

<212>PRT

<213>谷氨酸棒状杆菌

<400>42

Leu Lys Glu Thr Phe Leu Ala Arg Gln Lys Lys Ser Ala Ala Ser Ala

1 5 10 15

Trp Glu Arg Trp Pro Lys Arg Ala Ile Ala Leu Phe Val Leu Ile Val

20 25 30

Val Gly Val Tyr Ala Leu Val Leu Leu Thr Gly Asp Arg Ser Ala Thr

35 40 45

Pro Lys Leu Gly Ile Asp Leu Gln Gly Gly Thr Arg Val Thr Leu Val

50 55 60

Pro Gln Gly Gln Asp Pro Thr Gln Asp Gln Leu Asn Gln Ala Arg Thr

65 70 75 80

Ile Leu Glu Asn Arg Val Asn Gly Met Gly Val Ser Gly Ala Ser Val

85 90 95

Val Ala Asp Gly Asn Thr Leu Val Ile Thr Val Pro Gly Glu Asn Thr

100 105 110

Ala Gln Ala Gln Ser Leu Gly Gln Thr Ser Gln Leu Leu Phe Arg Pro

115 120 125

Val Gly Gln Ala Gly Met Pro Asp Met Thr Thr Leu Met Pro Glu Leu

130 135 140

Glu Glu Met Ala Asn Arg Trp Val Glu Tyr Gly Val Ile Thr Glu Glu

145 150 155 160

Gln Ala Asn Ala Ser Leu Glu Glu Met Asn Thr Ala Val Ala Ser Thr

165 170 175

Thr Ala Val Glu Gly Glu Glu Ala Thr Glu Pro Glu Pro Val Thr Val

180 185 190

Ser Ala Thr Pro Met Asp Glu Pro Ala Asn Ser Ile Glu Ala Thr Gln

195 200 205

Arg Arg Gln Glu Ile Thr Asp Met Leu Arg Thr Asp Arg Gln Ser Thr

210 215 220

Asp Pro Thr Val Gln Ile Ala Ala Ser Ser Leu Met Gln Cys Thr Thr

225 230 235 240

Asp Glu Met Asp Pro Leu Ala Gly Thr Asp Asp Pro Arg Leu Pro Leu

245 250 255

Val Ala Cys Asp Pro Ala Val Gly Gly Val Tyr Val Leu Asp Pro Ala

260 265 270

Pro Leu Leu Asn Gly Glu Thr Asp Glu Glu Asn Gly Ala Arg Leu Thr

275 280 285

Gly Asn Glu Ile Asp Thr Asn Arg Pro Ile Thr Gly Gly Phe Asn Ala

290 295 300

Gln Ser Gly Gln Met Glu Ile Ser Phe Ala Phe Lys Ser Gly Asp Gly

305 310 315 320

Glu Glu Gly Ser Ala Thr Trp Ser Ser Leu Thr Ser Gln Tyr Leu Gln

325 330 335

Gln Gln Ile Ala Ile Thr Leu Asp Ser Gln Val Ile Ser Ala Pro Val

340 345 350

Ile Gln Ser Ala Thr Pro Val Gly Ser Ala Thr Ser Ile Thr Gly Asp

355 360 365

Phe Thr Gln Thr Glu Ala Gln Asp Leu Ala Asn Asn Leu Arg Tyr Gly

370 375 380

Ala Leu Pro Leu Ser Phe Ala Gly Glu Asn Gly Glu Arg Gly Gly Thr

385 390 395 400

Thr Thr Thr Val Pro Pro Ser Leu Gly Ala Ala Ser Leu Lys Ala Gly

405 410 415

Leu Ile Ala Gly Ile Val Gly Ile Ala Leu Val Ala Ile Phe Val Phe

420 425 430

Ala Tyr Tyr Arg Val Phe Gly Phe Val Ser Leu Phe Thr Leu Phe Ala

435 440 445

Ala Gly Val Leu Val Tyr Gly Leu Leu Val Leu Leu Gly Arg Trp Ile

450 455 460

Gly Tyr Ser Leu Asp Leu Ala Gly Ile Ala Gly Leu Ile Ile Gly Ile

465 470 475 480

Gly Thr Thr Ala Asp Ser Phe Val Val Phe Tyr Glu Arg Ile Lys Asp

485 490 495

Glu Ile Arg Glu Gly Arg Ser Phe Arg Ser Ala Val Pro Arg Ala Trp

500 505 510

Glu Ser Ala Lys Arg Thr Ile Val Thr Gly Asn Met Val Thr Leu Leu

515 520 525

Gly Ala Ile Val Ile Tyr Leu Leu Ala Val Gly Glu Val Lys Gly Phe

530 535 540

Ala Phe Thr Leu Gly Leu Thr Thr Val Phe Asp Leu Val Val Thr Phe

545 550 555 560

Leu Ile Thr Ala Pro Leu Val Ile Leu Ala Ser Arg Asn Pro Phe Phe

565 570 575

Ala Lys Ser Ser Val Asn Gly Met Gly Arg Val Met Lys Leu Val Glu

580 585 590

Glu Arg Arg Ala Asn Gly Glu Leu Asp Glu Pro Glu Tyr Leu Lys Lys

595 600 605

Ile His Ala Lys Asn Ala Ala Ala Asp Lys Ala Ser Thr Asp Asn Ser

610 615 620

Ser Thr Asp Asn Ser Glu Ala Pro Gly Thr Asp Thr Asn Gln Glu Glu

625 630 635 640

Glu Lys

<210>43

<211>1909

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1879)

<223>RXA01596

<400>43

tcccaggtca gcggggtaat tcgaaaacca ttcgaacaat tttcgaggat ttagaaaaaa 60

cgttcgcata aattgttaga actgatgtac actttgaggc atg ctc gta gac att 115

Met Leu Val Asp Ile

1 5

gct att gag aac ctc gga gtt att cca gcg gcc tca gct gag ttc agc 163

Ala Ile Glu Asn Leu Gly Val Ile Pro Ala Ala Ser Ala Glu Phe Ser

10 15 20

tca ggt tta aca gtg ctc acc ggt gag acc ggc gcc gga aag acc atg 211

Ser Gly Leu Thr Val Leu Thr Gly Glu Thr Gly Ala Gly Lys Thr Met

25 30 35

gta gtg aca ggt tta cgc ctg tta tcc ggc ggt cgc gcc gac gct tca 259

Val Val Thr Gly Leu Arg Leu Leu Ser Gly Gly Arg Ala Asp Ala Ser

40 45 50

cgc gtg cgc aca gga tcc cct caa gct gtt gtg gag ggg cgc ttt gtt 307

Arg Val Arg Thr Gly Ser Pro Gln Ala Val Val Glu Gly Arg Phe Val

55 60 65

acg caa ggc gtg ccc tgc gac att gtc gaa cgt gca acc gga atc gtt 355

Thr Gln Gly Val Pro Cys Asp Ile Val Glu Arg Ala Thr Gly Ile Val

70 75 80 85

tcg aac gcc gga ggt gcc gca gat gaa aat gga gag ttt tta gct gtc 403

Ser Asr Ala Gly Gly Ala Ala Asp Glu Asn Gly Glu Phe Leu Ala Val

90 95 100

cgt tcc gtc ggc gcc aac ggc cgt tca aaa gct cat ctc ggt ggt cgc 451

Arg Ser Val Gly Ala Asn Gly Arg Ser Lys Ala His Leu Gly Gly Arg

105 110 115

tcc gta cct gcg gca acg ctg tcc gag ttc tct gat gag ctg ttg acc 499

Ser Val Pro Ala Ala Thr Leu Ser Glu Phe Ser Asp Glu Leu Leu Thr

120 125 130

atc cac ggt caa aat gac caa ctc cgg ttg ctc tcc cca gaa cgc caa 547

Ile His Gly Gln Asn Asp Gln Leu Arg Leu Leu Ser Pro Glu Arg Gln

135 140 145

cta gag gcg ctt gat cgt ttt gat cca gag ctg gcc caa ctg cgc aaa 595

Leu Glu Ala Leu Asp Arg Phe Asp Pro Glu Leu Ala Gln Leu Arg Lys

150 155 160 165

aac tac aac gcc aag tac ctc act tgg aag tcc ttg gat aaa gat ctg 643

Asn Tyr Asn Ala Lys Tyr Leu Thr Trp Lys Ser Leu Asp Lys Asp Leu

170 175 180

cag aag cgc ctg agt agt agg cga gag ctg gct caa gaa gtc gat cgc 691

Gln Lys Arg Leu Ser Ser Arg Arg Glu Leu Ala Gln Glu Val Asp Arg

185 190 195

ctg caa ttc gcg att aat gag atc gag gaa gtc tcg cca cag cca ggc 739

Leu Gln Phe Ala Ile Asn Glu Ile Glu Glu Val Ser Pro Gln Pro Gly

200 205 210

gaa gac gcc gaa ctg gtt gag cag atc cgc agg ctc cag gac gtg gac 787

Glu Asp Ala Glu Leu Val Glu Gln Ile Arg Arg Leu Gln Asp Val Asp

215 220 225

acc ctg cgg gag caa gct gca acc gca ttg gct gcg att gat ggt gcc 835

Thr Leu Arg Glu Gln Ala Ala Thr Ala Leu Ala Ala Ile Asp Gly Ala

230 235 240 245

ggc tct ctc agc gac gcc atg ggt ggt tcc ggc ggc ttt gat gaa tcc 883

Gly Ser Leu Ser Asp Ala Met Gly Gly Ser Gly Gly Phe Asp Glu Ser

250 255 260

cag gag tca gcc tct gac cag ctc ggc cag gcg gag tcc gcg ctg gca 931

Gln Glu Ser Ala Ser Asp Gln Leu Gly Gln Ala Glu Ser Ala Leu Ala

265 270 275

ggc agt gat gac tca aag ctg aaa gat att gcc gtt cag ctt gcg gaa 979

Gly Ser Asp Asp Ser Lys Leu Lys Asp Ile Ala Val Gln Leu Ala Glu

280 285 290

atc acc agc cag ctc agc caa gtg tcc atg gaa ttg ggc ggg ttc ctc 1027

Ile Thr Ser Gln Leu Ser Gln Val Ser Met Glu Leu Gly Gly Phe Leu

295 300 305

tct gat ctc ccc gca gac ccc caa gca ctc gat gac atg ctc acc cgc 1075

Ser Asp Leu Pro Ala Asp Pro Gln Ala Leu Asp Asp Met Leu Thr Arg

310 315 320 325

caa cag caa ttg aaa ctg ctc acg cgt aaa tac gct gca gat att gac 1123

Gln Gln Gln Leu Lys Leu Leu Thr Arg Lys Tyr Ala Ala Asp Ile Asp

330 335 340

ggc gtg att gag tgg cag cgg aaa gcc caa atc cgc cta gac agc att 1171

Gly Val Ile Glu Trp Gln Arg Lys Ala Gln Ile Arg Leu Asp Ser Ile

345 350 355

gac att tcc tcc gaa gcg ctt gac aag ctg aaa gaa gac gcg aaa aag 1219

Asp Ile Ser Ser Glu Ala Leu Asp Lys Leu Lys Glu Asp Ala Lys Lys

360 365 370

gcg cag gcc tcc atg atg cgt gcc gct aag aag ctt tca gct gtc cgt 1267

Ala Gln Ala Ser Met Met Arg Ala Ala Lys Lys Leu Ser Ala Val Arg

375 380 385

gca aag gca gca acc aag ttg ggg aca act gtc acc gag gag ctt cag 1315

Ala Lys Ala Ala Thr Lys Leu Gly Thr Thr Val Thr Glu Glu Leu Gln

390 395 400 405

ggc ctg gcc atg caa aaa gcc cgc ttt gag gtt gct ttg acc tcc att 1363

Gly Leu Ala Met Gln Lys Ala Arg Phe Glu Val Ala Leu Thr Ser Ile

410 415 420

gag gcg tgc gcc agc ggt atc gac cag gtg gaa ttc cag ctc gca gca 1411

Glu Ala Cys Ala Ser Gly Ile Asp Gln Val Glu Phe Gln Leu Ala Ala

425 430 435

aat gcc ttt gca cag cct cgt cca ctt gca tcc tct gcg tct ggt ggt 1459

Asn Ala Phe Ala Gln Pro Arg Pro Leu Ala Ser Ser Ala Ser Gly Gly

440 445 450

gaa ctt tcc cgc gtt atg ttg gcg ctc gag gtg atc ttg gct gct gga 1507

Glu Leu Ser Arg Val Met Leu Ala Leu Glu Val Ile Leu Ala Ala Gly

455 460 465

acc acg ggc acc acc ttg gtg ttc gac gag gtt gat gca ggt gtg ggc 1555

Thr Thr Gly Thr Thr Leu Val Phe Asp Glu Val Asp Ala Gly Val Gly

470 475 480 485

gga cgc gca gcg gtg gaa atc ggt cgc cgc ctg gcc cgc ctt gcc acc 1603

Gly Arg Ala Ala Val Glu Ile Gly Arg Arg Leu Ala Arg Leu Ala Thr

490 495 500

aaa aac caa gtc atc gtg gtc acc cat ctc cca cag gtc gct gct tac 1651

Lys Asn Gln Val Ile Val Val Thr His Leu Pro Gln Val Ala Ala Tyr

505 510 515

gcc gac acg cac ctg cac gtt gcc aag aat gta gga gaa gcc tcc gtg 1699

Ala Asp Thr His Leu His Val Ala Lys Asn Val Gly Glu Ala Ser Val

520 525 530

acc tca gga gtg gag tca ctg acc ttc gac cga cgc gtg gaa gag ctc 1747

Thr Ser Gly Val Glu Ser Leu Thr Phe Asp Arg Arg Val Glu Glu Leu

535 540 545

tcc cgc atg ctc gct ggc ctc gac gac acc gcc acc ggc cga gcc cac 1795

Ser Arg Met Leu Ala Gly Leu Asp Asp Thr Ala Thr Gly Arg Ala His

550 555 560 565

gca acg gag ctg ctc gag cgt gca cag cgt gaa aag gaa gat att aac 1843

Ala Thr Glu Leu Leu Glu Arg Ala Gln Arg Glu Lys Glu Asp Ile Asn

570 575 580

gag gag cga gta gaa cca ctt ctc gcc gcc agt gca taagagtttt 1889

Glu Glu Arg Val Glu Pro Leu Leu Ala Ala Ser Ala

585 590

cttggaattt tttaggcgcg 1909

<210>44

<211>593

<212>PRT

<213>谷氨酸棒状杆菌

<400>44

Met Leu Val Asp Ile Ala Ile Glu Asn Leu Gly Val Ile Pro Ala Ala

1 5 10 15

Ser Ala Glu Phe Ser Ser Gly Leu Thr Val Leu Thr Gly Glu Thr Gly

20 25 30

Ala Gly Lys Thr Met Val Val Thr Gly Leu Arg Leu Leu Ser Gly Gly

35 40 45

Arg Ala Asp Ala Ser Arg Val Arg Thr Gly Ser Pro Gln Ala Val Val

50 55 60

Glu Gly Arg Phe Val Thr Gln Gly Val Pro Cys Asp Ile Val Glu Arg

65 70 75 80

Ala Thr Gly Ile Val Ser Asn Ala Gly Gly Ala Ala Asp Glu Asn Gly

85 90 95

Glu Phe Leu Ala Val Arg Ser Val Gly Ala Asn Gly Arg Ser Lys Ala

100 105 110

His Leu Gly Gly Arg Ser Val Pro Ala Ala Thr Leu Ser Glu Phe Ser

115 120 125

Asp Glu Leu Leu Thr Ile His Gly Gln Asn Asp Gln Leu Arg Leu Leu

130 135 140

Ser Pro Glu Arg Gln Leu Glu Ala Leu Asp Arg Phe Asp Pro Glu Leu

145 150 155 160

Ala Gln Leu Arg Lys Asn Tyr Asn Ala Lys Tyr Leu Thr Trp Lys Ser

165 170 175

Leu Asp Lys Asp Leu Gln Lys Arg Leu Ser Ser Arg Arg Glu Leu Ala

180 185 190

Gln Glu Val Asp Arg Leu Gln Phe Ala Ile Asn Glu Ile Glu Glu Val

195 200 205

Ser Pro Gln Pro Gly Glu Asp Ala Glu Leu Val Glu Gln Ile Arg Arg

210 215 220

Leu Gln Asp Val Asp Thr Leu Arg Glu Gln Ala Ala Thr Ala Leu Ala

225 230 235 240

Ala Ile Asp Gly Ala Gly Ser Leu Ser Asp Ala Met Gly Gly Ser Gly

245 250 255

Gly Phe Asp Glu Ser Gln Glu Ser Ala Ser Asp Gln Leu Gly Gln Ala

260 265 270

Glu Ser Ala Leu Ala Gly Ser Asp Asp Ser Lys Leu Lys Asp Ile Ala

275 280 285

Val Gln Leu Ala Glu Ile Thr Ser Gln Leu Ser Gln Val Ser Met Glu

290 295 300

Leu Gly Gly Phe Leu Ser Asp Leu Pro Ala Asp Pro Gln Ala Leu Asp

305 310 315 320

Asp Met Leu Thr Arg Gln Gln Gln Leu Lys Leu Leu Thr Arg Lys Tyr

325 330 335

Ala Ala Asp Ile Asp Gly Val Ile Glu Trp Gln Arg Lys Ala Gln Ile

340 345 350

Arg Leu Asp Ser Ile Asp Ile Ser Ser Glu Ala Leu Asp Lys Leu Lys

355 360 365

Glu Asp Ala Lys Lys Ala Gln Ala Ser Met Met Arg Ala Ala Lys Lys

370 375 380

Leu Ser Ala Val Arg Ala Lys Ala Ala Thr Lys Leu Gly Thr Thr Val

385 390 395 400

Thr Glu Glu Leu Gln Gly Leu Ala Met Gln Lys Ala Arg Phe Glu Val

405 410 415

Ala Leu Thr Ser Ile Glu Ala Cys Ala Ser Gly Ile Asp Gln Val Glu

420 425 430

Phe Gln Leu Ala Ala Asn Ala Phe Ala Gln Pro Arg Pro Leu Ala Ser

435 440 445

Ser Ala Ser Gly Gly Glu Leu Ser Arg Val Met Leu Ala Leu Glu Val

450 455 460

Ile Leu Ala Ala Gly Thr Thr Gly Thr Thr Leu Val Phe Asp Glu Val

465 470 475 480

Asp Ala Gly Val Gly Gly Arg Ala Ala Val Glu Ile Gly Arg Arg Leu

485 490 495

Ala Arg Leu Ala Thr Lys Asn Gln Val Ile Val Val Thr His Leu Pro

500 505 510

Gln Val Ala Ala Tyr Ala Asp Thr His Leu His Val Ala Lys Asn Val

515 520 525

Gly Glu Ala Ser Val Thr Ser Gly Val Glu Ser Leu Thr Phe Asp Arg

530 535 540

Arg Val Glu Glu Leu Ser Arg Met Leu Ala Gly Leu Asp Asp Thr Ala

545 550 555 560

Thr Gly Arg Ala His Ala Thr Glu Leu Leu Glu Arg Ala Gln Arg Glu

565 570 575

Lys Glu Asp Ile Asn Glu Glu Arg Val Glu Pro Leu Leu Ala Ala Ser

580 585 590

Ala

<210>45

<211>265

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(235)

<223>RXA01651

<400>45

caatctctaa ggagaaagtt tatgacaaat aggacctgac ccctgtttgg tagacaccta 60

acatcccaac attctgggac agaaaggtaa cctacctatc atg cca acc aag acc 115

Met Pro Thr Lys Thr

1 5

tac tcc gag gag ttc aaa cgc gac gcc gtt gct ttg tac gag aac tcc 163

Tyr Ser Glu Glu Phe Lys Arg Asp Ala Val Ala Leu Tyr Glu Asn Ser

10 15 20

gat ggg gcc tca ctc caa cag atc gcc aac gat ctc ggc atc aac cga 211

Asp Gly Ala Ser Leu Gln Gln Ile Ala Asn Asp Leu Gly Ile Asn Arg

25 30 35

gta acc ctg aaa aac ttc gat caa taaatacggt gcgcatgcct caaccaacac 265

Val Thr Leu Lys Asn Phe Asp Gln

40 45

<210>46

<211>45

<212>PRT

<213>谷氨酸棒状杆菌

<400>46

Met Pro Thr Lys Thr Tyr Ser Glu Glu Phe Lys Arg Asp Ala Val Ala

1 5 10 15

Leu Tyr Glu Asn Ser Asp Gly Ala Ser Leu Gln Gln Ile Ala Asn Asp

20 25 30

Leu Gly Ile Asn Arg Val Thr Leu Lys Asn Phe Asp Gln

35 40 45

<210>47

<211>538

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(508)

<223>RXA01710

<400>47

tctcggcgct aatctggttt attggtgata tccgagccaa gggaactccg agctcaccca 60

ttaccactga tccaacgcac gaccatcttg agaggacagc atg aca gac ttc aaa 115

Met Thr Asp Phe Lys

1 5

ctc atc agc gat acc gag tgg cgc gaa cgc ctc acc ccg cag gaa ttc 163

Leu Ile Ser Asp Thr Glu Trp Arg Glu Arg Leu Thr Pro Gln Glu Phe

10 15 20

cat gtc ctc cgc gaa gcc ggc acc gaa cca cct cac gtc ggt gaa tac 211

His Val Leu Arg Glu Ala Gly Thr Glu Pro Pro His Val Gly Glu Tyr

25 30 35

acc aac acc acc acc gaa ggt gtg tac tcc tgt cgc gcc tgt ggt gaa 259

Thr Asn Thr Thr Thr Glu Gly Val Tyr Ser Cys Arg Ala Cys Gly Glu

40 45 50

gag tta ttc cgc tcc acc gag aag ttt gaa tcc cac tgc ggt tgg cct 307

Glu Leu Phe Arg Ser Thr Glu Lys Phe Glu Ser His Cys Gly Trp Pro

55 60 65

tcc ttc ttc tcc cca ctt gct ggc gac aaa atc att gag aag gaa gat 355

Ser Phe Phe Ser Pro Leu Ala Gly Asp Lys Ile Ile Glu Lys Glu Asp

70 75 80 85

ctt tcc ctc ggt atg cgt cgc gtt gag att ctg tgc gct aac tgc ggc 403

Leu Ser Leu Gly Met Arg Arg Val Glu Ile Leu Cys Ala Asn Cys Gly

90 95 100

tct cac atg ggt cac gtc ttc gaa ggc gaa ggc tac gac acc ccc acc 451

Ser His Met Gly His Val Phe Glu Gly Glu Gly Tyr Asp Thr Pro Thr

105 110 115

gat ctt cgt tac tgc att aac tcc atc agc ttg aag ctg gaa gaa aag 499

Asp Leu Arg Tyr Cys Ile Asn Ser Ile Ser Leu Lys Leu Glu Glu Lys

120 125 130

cca gtt tcc taagcttccg agcacgaaac gagccttggc 538

Pro Val Ser

135

<210>48

<211>136

<212>PRT

<213>谷氨酸棒状杆菌

<400>48

Met Thr Asp Phe Lys Leu Ile Ser Asp Thr Glu Trp Arg Glu Arg Leu

1 5 10 15

Thr Pro Gln Glu Phe His Val Leu Arg Glu Ala Gly Thr Glu Pro Pro

20 25 30

His Val Gly Glu Tyr Thr Asn Thr Thr Thr Glu Gly Val Tyr Ser Cys

35 40 45

Arg Ala Cys Gly Glu Glu Leu Phe Arg Ser Thr Glu Lys Phe Glu Ser

50 55 60

His Cys Gly Trp Pro Ser Phe Phe Ser Pro Leu Ala Gly Asp Lys Ile

65 70 75 80

Ile Glu Lys Glu Asp Leu Ser Leu Gly Met Arg Arg Val Glu Ile Leu

85 90 95

Cys Ala Asn Cys Gly Ser His Met Gly His Val Phe Glu Gly Glu Gly

100 105 110

Tyr Asp Thr Pro Thr Asp Leu Arg Tyr Cys Ile Asn Ser Ile Ser Leu

115 120 125

Lys Leu Glu Glu Lys Pro Val Ser

130 135

<210>49

<211>1417

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1387)

<223>RXA01852

<400>49

aaccaccacc atgcgtgcag aacgcactgg taaccctttc ttgctggcac tgtagggcta 60

agttccgtac tacttcttcg aataggtatc gttaataatc gtg agt caa aac aag 115

Val Ser Gln Asn Lys

1 5

tcc aag tct gaa aag ctt cag tca ttt gct gca ccc aag ggt gtt cct 163

Ser Lys Ser Glu Lys Leu Gln Ser Phe Ala Ala Pro Lys Gly Val Pro

10 15 20

gat tac gcc cca cca aaa tct gca gcg ttt tta gca gtc cgt gat gcc 211

Asp Tyr Ala Pro Pro Lys Ser Ala Ala Phe Leu Ala Val Arg Asp Ala

25 30 35

ttt gtt aat caa gca cat aag gcc ggg ttt gag cat att gag ctg ccg 259

Phe Val Asn Gln Ala His Lys Ala Gly Phe Glu His Ile Glu Leu Pro

40 45 50

atc ttt gaa gac acc ggc ttg ttt gcg cgt ggt gtt ggt gag tcc act 307

Ile Phe Glu Asp Thr Gly Leu Phe Ala Arg Gly Val Gly Glu Ser Thr

55 60 65

gac gta gtg agc aag gaa atg tac acc ttc gct gat cgt ggc gag cgc 355

Asp Val Val Ser Lys Glu Met Tyr Thr Phe Ala Asp Arg Gly Glu Arg

70 75 80 85

tct gtc acg ctg cgc cca gaa ggc act gca ggc gtg atg cgt gca gtt 403

Ser Val Thr Leu Arg Pro Glu Gly Thr Ala Gly Val Met Arg Ala Val

90 95 100

att gaa cac agc ctg gac cgt gga cag ctt ccc gta aag ctg aac tac 451

Ile Glu His Ser Leu Asp Arg Gly Gln Leu Pro Val Lys Leu Asn Tyr

105 110 115

gcc gga cca ttc ttc cgt tat gag cgt cct cag gca ggg cgt tac cgt 499

Ala Gly Pro Phe Phe Arg Tyr Glu Arg Pro Gln Ala Gly Arg Tyr Arg

120 125 130

cag ctt cag caa gta ggc gta gag gca att ggt gtg gat gat cca gcg 547

Gln Leu Gln Gln Val Gly Val Glu Ala Ile Gly Val Asp Asp Pro Ala

135 140 145

ctt gat gcg gag atc att gcg ctt gct gat cgt tct tac cgc agc ttg 595

Leu Asp Ala Glu Ile Ile Ala Leu Ala Asp Arg Ser Tyr Arg Ser Leu

150 155 160 165

ggg ctg cag gat ttc cgt ctg gag ctc acc agc ttg ggt gat cgt cac 643

Gly Leu Gln Asp Phe Arg Leu Glu Leu Thr Ser Leu Gly Asp Arg His

170 175 180

tgc cgt ccc gag tat cgt cag aag ctg cag gat ttc ttg ttt gca ctt 691

Cys Arg Pro Glu Tyr Arg Gln Lys Leu Gln Asp Phe Leu Phe Ala Leu

185 190 195

cct ttg gat gag gaa acc cgc aag cgc gca gag atc aac cca ctt cgg 739

Pro Leu Asp Glu Glu Thr Arg Lys Arg Ala Glu Ile Asn Pro Leu Arg

200 205 210

gtg ttg gat gat aag cgt cct gaa gtc caa gag atg act gcg gat gca 787

Val Leu Asp Asp Lys Arg Pro Glu Val Gln Glu Met Thr Ala Asp Ala

215 220 225

cca ttg atg ctg gat cac ctt gat gca gag tgc cgt gag cac ttt gaa 835

Pro Leu Met Leu Asp His Leu Asp Ala Glu Cys Arg Glu His Phe Glu

230 235 240 245

aca gtg act ggt ttg ctc gat gac atg ggt gtt cca tat gtg att aac 883

Thr Val Thr Gly Leu Leu Asp Asp Met Gly Val Pro Tyr Val Ile Asn

250 255 260

cca cgc atg gtt cgt ggt ttg gat tac tac acc aag act tgt ttt gag 931

Pro Arg Met Val Arg Gly Leu Asp Tyr Tyr Thr Lys Thr Cys Phe Glu

265 270 275

ttc gtt cac gat ggc ctg ggc gca cag tct ggc att ggt ggc ggc gga 979

Phe Val His Asp Gly Leu Gly Ala Gln Ser Gly Ile Gly Gly Gly Gly

280 285 290

cgc tac gac ggt ctg atg gca cag ctt ggc gga cag gat ctg tct ggc 1027

Arg Tyr Asp Gly Leu Met Ala Gln Leu Gly Gly Gln Asp Leu Ser Gly

295 300 305

atc ggc tat ggc ctg ggt gtg gat cgc acc atg ttg gct ctg gaa gct 1075

Ile Gly Tyr Gly Leu Gly Val Asp Arg Thr Met Leu Ala Leu Glu Ala

310 315 320 325

gaa ggt gtg act gtt ggt gct gag cgt cgc gtt gat gtg tac ggc gtt 1123

Glu Gly Val Thr Val Gly Ala Glu Arg Arg Val Asp Val Tyr Gly Val

330 335 340

cca ctg ggc aag gat gct aag aag gct ctt gct gga atc gtg aac acg 1171

Pro Leu Gly Lys Asp Ala Lys Lys Ala Leu Ala Gly Ile Val Asn Thr

345 350 355

ctg cgc gct gcg ggt att tcc acc gat atg tct tac ggc gac cgt ggc 1219

Leu Arg Ala Ala Gly Ile Ser Thr Asp Met Ser Tyr Gly Asp Arg Gly

360 365 370

ctg aag ggt gcc atg aag ggc gct gac cgc tcc aac gcg ttg tac acc 1267

Leu Lys Gly Ala Met Lys Gly Ala Asp Arg Ser Asn Ala Leu Tyr Thr

375 380 385

ttg gtg ctg ggc gag cag gag ctg gag aac aac acc atc gcg gtg aag 1315

Leu Val Leu Gly Glu Gln Glu Leu Glu Asn Asn Thr Ile Ala Val Lys

390 395 400 405

gat atg cgt gcg cat gag cag cac gat gtc gca ttg gac gag gtt gtg 1363

Asp Met Arg Ala His Glu Gln His Asp Val Ala Leu Asp Glu Val Val

410 415 420

gcc ttt ttg cag ggg aaa ctt att taaataattc ataagtaaaa aaccgtcaat 1417

Ala Phe Leu Gln Gly Lys Leu Ile

425

<210>50

<211>429

<212>PRT

<213>谷氨酸棒状杆菌

<400>50

Val Ser Gln Asn Lys Ser Lys Ser Glu Lys Leu Gln Ser Phe Ala Ala

1 5 10 15

Pro Lys Gly Val Pro Asp Tyr Ala Pro Pro Lys Ser Ala Ala Phe Leu

20 25 30

Ala Val Arg Asp Ala Phe Val Asn Gln Ala His Lys Ala Gly Phe Glu

35 40 45

His Ile Glu Leu Pro Ile Phe Glu Asp Thr Gly Leu Phe Ala Arg Gly

50 55 60

Val Gly Glu Ser Thr Asp Val Val Ser Lys Glu Met Tyr Thr Phe Ala

65 70 75 80

Asp Arg Gly Glu Arg Ser Val Thr Leu Arg Pro Glu Gly Thr Ala Gly

85 90 95

Val Met Arg Ala Val Ile Glu His Ser Leu Asp Arg Gly Gln Leu Pro

100 105 110

Val Lys Leu Asn Tyr Ala Gly Pro Phe Phe Arg Tyr Glu Arg Pro Gln

115 120 125

Ala Gly Arg Tyr Arg Gln Leu Gln Gln Val Gly Val Glu Ala Ile Gly

130 135 140

Val Asp Asp Pro Ala Leu Asp Ala Glu Ile Ile Ala Leu Ala Asp Arg

145 150 155 160

Ser Tyr Arg Ser Leu Gly Leu Gln Asp Phe Arg Leu Glu Leu Thr Ser

165 170 175

Leu Gly Asp Arg His Cys Arg Pro Glu Tyr Arg Gln Lys Leu Gln Asp

180 185 190

Phe Leu Phe Ala Leu Pro Leu Asp Glu Glu Thr Arg Lys Arg Ala Glu

195 200 205

Ile Asn Pro Leu Arg Val Leu Asp Asp Lys Arg Pro Glu Val Gln Glu

210 215 220

Met Thr Ala Asp Ala Pro Leu Met Leu Asp His Leu Asp Ala Glu Cys

225 230 235 240

Arg Glu His Phe Glu Thr Val Thr Gly Leu Leu Asp Asp Met Gly Val

245 250 255

Pro Tyr Val Ile Asn Pro Arg Met Val Arg Gly Leu Asp Tyr Tyr Thr

260 265 270

Lys Thr Cys Phe Glu Phe Val His Asp Gly Leu Gly Ala Gln Ser Gly

275 280 285

Ile Gly Gly Gly Gly Arg Tyr Asp Gly Leu Met Ala Gln Leu Gly Gly

290 295 300

Gln Asp Leu Ser Gly Ile Gly Tyr Gly Leu Gly Val Asp Arg Thr Met

305 310 315 320

Leu Ala Leu Glu Ala Glu Gly Val Thr Val Gly Ala Glu Arg Arg Val

325 330 335

Asp Val Tyr Gly Val Pro Leu Gly Lys Asp Ala Lys Lys Ala Leu Ala

340 345 350

Gly Ile Val Asn Thr Leu Arg Ala Ala Gly Ile Ser Thr Asp Met Ser

355 360 365

Tyr Gly Asp Arg Gly Leu Lys Gly Ala Met Lys Gly Ala Asp Arg Ser

370 375 380

Asn Ala Leu Tyr Thr Leu Val Leu Gly Glu Gln Glu Leu Glu Asn Asn

385 390 395 400

Thr Ile Ala Val Lys Asp Met Arg Ala His Glu Gln His Asp Val Ala

405 410 415

Leu Asp Glu Val Val Ala Phe Leu Gln Gly Lys Leu Ile

420 425

<210>51

<211>955

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(925)

<223>RXA01913

<400>51

acctgtacga tcacttttta gacgggcggg tagggctact gtgccctaac ctaagcttgt 60

aaagcattaa ttatccatac ataaggagga tcgccccgta atg gcg aac tac acc 115

Met Ala Asn Tyr Thr

1 5

gct gcg gat gtt aag aag ctc cgc gaa ctc acc ggt tcc ggc atg ctc 163

Ala Ala Asp Val Lys Lys Leu Arg Glu Leu Thr Gly Ser Gly Met Leu

10 15 20

gat tgc aag aag gct ctg gag gag tcc gct ggc gac ttc gac aag gct 211

Asp Cys Lys Lys Ala Leu Glu Glu Ser Ala Gly Asp phe Asp Lys Ala

25 30 35

gtt gag atc ctg cgc gtc aag ggc gca aag gac gtc gga aag cgt gca 259

Val Glu Ile Leu Arg Val Lys Gly Ala Lys Asp Val Gly Lys Arg Ala

40 45 50

gag cgt aac gct acc gaa ggt ctc gtt gca gtt tct ggc aac acc atg 307

Glu Arg Asn Ala Thr Glu Gly Leu Val Ala Val Ser Gly Asn Thr Met

55 60 65

gtc gag gtc aac tct gag acc gac ttc gtt gca aag aac tct gac ttc 355

Val Glu Val Asn Ser Glu Thr Asp Phe Val Ala Lys Asn Ser Asp Phe

70 75 80 85

aag gaa ttc gct gca aag gtt gca gac gca gca gca gct gca aag gct 403

Lys Glu Phe Ala Ala Lys Val Ala Asp Ala Ala Ala Ala Ala Lys Ala

90 95 100

aac tcc cag gaa gag ctc gca gca gtt gac gtg gac gga cag acc gca 451

Asn Ser Gln Glu Glu Leu Ala Ala Val Asp Val Asp Gly Gln Thr Ala

105 110 115

gac gca gct ctg cag gag ttc tcc gca aag atc ggc gag aag ctt gag 499

Asp Ala Ala Leu Gln Glu Phe Ser Ala Lys Ile Gly Glu Lys Leu Glu

120 125 130

ctt cgt cgc gca gta acc ctc gag ggc gac aag acc gct gtt tac ctc 547

Leu Arg Arg Ala Val Thr Leu Glu Gly Asp Lys Thr Ala Val Tyr Leu

135 140 145

cac cag cgt tcc gct gac ctg cca cca gca gtt ggc gtt ttg gtt gct 595

His Gln Arg Ser Ala Asp Leu Pro Pro Ala Val Gly Val Leu Val Ala

150 155 160 165

ttc acc ggt gaa ggt gaa gca gct gag gca gct gca cgt cag gct gca 643

Phe Thr Gly Glu Gly Glu Ala Ala Glu Ala Ala Ala Arg Gln Ala Ala

170 175 180

atg cag att gct gct ctg aag gct tct tac ctc acc cgt gag gac gtt 691

Met Gln Ile Ala Ala Leu Lys Ala Ser Tyr Leu Thr Arg Glu Asp Val

185 190 195

cct gca gag atc atc gag aag gag cgc tcc atc gct gag cag atc act 739

Pro Ala Glu Ile Ile Glu Lys Glu Arg Ser Ile Ala Glu Gln Ile Thr

200 205 210

cgc gaa gag ggc aag cca gag cag gct atc cct aag atc gtt gag ggt 787

Arg Glu Glu Gly Lys Pro Glu Gln Ala Ile Pro Lys Ile Val Glu Gly

215 220 225

cgt ttg aat ggc ttc tac aag gag aac gta ctt ctt gag cag tcc tcg 835

Arg Leu Asn Gly Phe Tyr Lys Glu Asn Val Leu Leu Glu Gln Ser Ser

230 235 240 245

gta gct gac agc aag aag acc gtt aag gct ctt ctg gac gag gct ggc 883

Val Ala Asp Ser Lys Lys Thr Val Lys Ala Leu Leu Asp Glu Ala Gly

250 255 260

gtt acc gtc acc tcc ttc gct cgc ttc gag gtc ggc cag gct 925

Val Thr Val Thr Ser Phe Ala Arg Phe Glu Val Gly Gln Ala

265 270 275

taaggccact tgaaggttgt gggtgggtgt 955

<210>52

<211>275

<212>PRT

<213>谷氨酸棒状杆菌

<400>52

Met Ala Asn Tyr Thr Ala Ala Asp Val Lys Lys Leu Arg Glu Leu Thr

1 5 10 15

Gly Ser Gly Met Leu Asp Cys Lys Lys Ala Leu Glu Glu Ser Ala Gly

20 25 30

Asp Phe Asp Lys Ala Val Glu Ile Leu Arg Val Lys Gly Ala Lys Asp

35 40 45

Val Gly Lys Arg Ala Glu Arg Asn Ala Thr Glu Gly Leu Val Ala Val

50 55 60

Ser Gly Asn Thr Met Val Glu Val Asn Ser Glu Thr Asp Phe Val Ala

65 70 75 80

Lys Asn Ser Asp Phe Lys Glu Phe Ala Ala Lys Val Ala Asp Ala Ala

85 90 95

Ala Ala Ala Lys Ala Asn Ser Gln Glu Glu Leu Ala Ala Val Asp Val

100 105 110

Asp Gly Gln Thr Ala Asp Ala Ala Leu Gln Glu Phe Ser Ala Lys Ile

115 120 125

Gly Glu Lys Leu Glu Leu Arg Arg Ala Val Thr Leu Glu Gly Asp Lys

130 135 140

Thr Ala Val Tyr Leu His Gln Arg Ser Ala Asp Leu Pro Pro Ala Val

145 150 155 160

Gly Val Leu Val Ala Phe Thr Gly Glu Gly Glu Ala Ala Glu Ala Ala

165 170 175

Ala Arg Gln Ala Ala Met Gln Ile Ala Ala Leu Lys Ala Ser Tyr Leu

180 185 190

Thr Arg Glu Asp Val Pro Ala Glu Ile Ile Glu Lys Glu Arg Ser Ile

195 200 205

Ala Glu Gln Ile Thr Arg Glu Glu Gly Lys Pro Glu Gln Ala Ile Pro

210 215 220

Lys Ile Val Glu Gly Arg Leu Asn Gly Phe Tyr Lys Glu Asn Val Leu

225 230 235 240

Leu Glu Gln Ser Ser Val Ala Asp Ser Lys Lys Thr Val Lys Ala Leu

245 250 255

Leu Asp Glu Ala Gly Val Thr Val Thr Ser Phe Ala Arg Phe Glu Val

260 265 270

Gly Gln Ala

275

<210>53

<211>1747

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1717)

<223>RXA02145

<400>53

cactgccaca gctgccaatt accgttgatg aagagggcta cctcatcgcc gctggtaact 60

tcattgagcc actcggccct gcattctggg agcgtaagtc atg agt cta gct acc 115

Met Ser Leu Ala Thr

1 5

gtg gga aac aat ctt gat tcc cgt tac acc atg gcg tcg ggt atc cgt 163

Val Gly Asn Asn Leu Asp Ser Arg Tyr Thr Met Ala Ser Gly Ile Arg

10 15 20

cgc cag atc aac aag gtc ttc cca act cac tgg tcc ttc atg ctc ggc 211

Arg Gln Ile Asn Lys Val Phe Pro Thr His Trp Ser Phe Met Leu Gly

25 30 35

gag att gcg ctt tac agc ttc atc gtc ttg ctg ctg act ggt gtc tac 259

Glu Ile Ala Leu Tyr Ser Phe Ile Val Leu Leu Leu Thr Gly Val Tyr

40 45 50

ctg acc ctg ttc ttc gac cca tca atc acc aag gtc att tat gac ggc 307

Leu Thr Leu Phe Phe Asp Pro Ser Ile Thr Lys Val Ile Tyr Asp Gly

55 60 65

ggc tac ctc cca ctg aac ggt gtg gag atg tcc cgt gca tac gca act 355

Gly Tyr Leu Pro Leu Asn Gly Val Glu Met Ser Arg Ala Tyr Ala Thr

70 75 80 85

gcg ttg gat att tcc ttc gag gtt cgc ggt ggt ctg ttc atc cgc cag 403

Ala Leu Asp Ile Ser Phe Glu Val Arg Gly Gly Leu Phe Ile Arg Gln

90 95 100

atg cac cac tgg gca gcc ctg ctg ttc gtt gta tcc atg ctg gtt cac 451

Met His His Trp Ala Ala Leu Leu Phe Val Val Ser Met Leu Val His

105 110 115

atg ctc cgt att ttc ttc acc ggt gcg ttc cgt cgc cca cgt gaa gca 499

Met Leu Arg Ile Phe Phe Thr Gly Ala Phe Arg Arg Pro Arg Glu Ala

120 125 130

aac tgg atc atc ggt gtt gtt ctg atc atc ctg ggt atg gct gaa ggc 547

Asn Trp Ile Ile Gly Val Val Leu Ile Ile Leu Gly Met Ala Glu Gly

135 140 145

ttc atg ggt tac tcc ctg cct gat gac ctg ctc tct ggt gtt ggt ctt 595

Phe Met Gly Tyr Ser Leu Pro Asp Asp Leu Leu Ser Gly Val Gly Leu

150 155 160 165

cga atc atg tcc gcc atc atc gtt ggt ctt ccg atc ata ggt acc tgg 643

Arg Ile Met Ser Ala Ile Ile Val Gly Leu Pro Ile Ile Gly Thr Trp

170 175 180

atg cac tgg ctg atc ttc ggt gga gac ttc cca tcc gat ctg atg ctg 691

Met His Trp Leu Ile Phe Gly Gly Asp Phe Pro Ser Asp Leu Met Leu

185 190 195

gac cgc ttc tac atc gca cac gtt cta atc atc cca gct atc ctg ctt 739

Asp Arg Phe Tyr Ile Ala His Val Leu Ile Ile Pro Ala Ile Leu Leu

200 205 210

ggc ttg atc gca gct cac ctg gca ctt gtt tgg tac cag aag cac acc 787

Gly Leu Ile Ala Ala His Leu Ala Leu Val Trp Tyr Gln Lys His Thr

215 220 225

cag ttc cca ggc gct ggc cgc act gag aac aac gtg atc ggt atc cga 835

Gln Phe Pro Gly Ala Gly Arg Thr Glu Asn Asn Val Ile Gly Ile Arg

230 235 240 245

atc atg cct ctg ttc gca gtt aag gct gtt gct ttc ggc ctc atc gtc 883

Ile Met Pro Leu Phe Ala Val Lys Ala Val Ala Phe Gly Leu Ile Val

250 255 260

ttc ggt ttc ctc gca ctg ctt gct ggt gtc acc acc att aac gca att 931

Phe Gly Phe Leu Ala Leu Leu Ala Gly Val Thr Thr Ile Asn Ala Ile

265 270 275

tgg aat ctt gga ccg tac aac cct tca cag gtg tct gct ggt tcc cag 979

Trp Asn Leu Gly Pro Tyr Asn Pro Ser Gln Val Ser Ala Gly Ser Gln

280 285 290

cct gac gtt tac atg ctg tgg aca gat ggt gct gct cgt gtc atg ccg 1027

Pro Asp Val Tyr Met Leu Trp Thr Asp Gly Ala Ala Arg Val Met Pro

295 300 305

gca tgg gag ctc tac ctc ggt aac tac act att cca gca gtc ttc tgg 1075

Ala Trp Glu Leu Tyr Leu Gly Asn Tyr Thr Ile Pro Ala Val Phe Trp

310 315 320 325

gtt gct gtg atg ctg ggt atc ctc gtg gtt ctg ctt gtg act tac cca 1123

Val Ala Val Met Leu Gly Ile Leu Val Val Leu Leu Val Thr Tyr Pro

330 335 340

ttc att gag cgt aag ttc acc ggc gac gat gca cac cac aac ttg ctg 1171

Phe Ile Glu Arg Lys Phe Thr Gly Asp Asp Ala His His Asn Leu Leu

345 350 355

cag cgt cct cgc gat gtt cca gtc cgc acc tca ctc ggt gtc atg gcg 1219

Gln Arg Pro Arg Asp Val Pro Val Arg Thr Ser Leu Gly Val Met Ala

360 365 370

ctt gtc ttc tac atc ctg ctt acc gtt tct ggt ggt aac gat gtt tac 1267

Leu Val Phe Tyr Ile Leu Leu Thr Val Ser Gly Gly Asn Asp Val Tyr

375 380 385

gca atg cag ttc cat gtt tca ctg aac gcg atg acc tgg atc ggt cgt 1315

Ala Met Gln Phe His Val Ser Leu Asn Ala Met Thr Trp Ile Gly Arg

390 395 400 405

atc ggc ctc atc gtt gga cca gct att gca tac ttc atc act tac cga 1363

Ile Gly Leu Ile Val Gly Pro Ala Ile Ala Tyr Phe Ile Thr Tyr Arg

410 415 420

ctg tgc atc ggc ttg cag cgc tct gac cgc gag gtc ctg gag cac ggc 1411

Leu Cys Ile Gly Leu Gln Arg Ser Asp Arg Glu Val Leu Glu His Gly

425 430 435

atc gag acc ggt atc atc aag cag atg cca aat ggt gcc ttc att gaa 1459

Ile Glu Thr Gly Ile Ile Lys Gln Met Pro Asn Gly Ala Phe Ile Glu

440 445 450

gtt cac cag cca ctt ggc cca gtt gat gac cat ggt cac cca atc cca 1507

Val His Gln Pro Leu Gly Pro Val Asp Asp His Gly His Pro Ile Pro

455 460 465

ctg cca tac gct ggc gct gcg gtt cca aag cag atg aac cag ctt ggt 1555

Leu Pro Tyr Ala Gly Ala Ala Val Pro Lys Gln Met Asn Gln Leu Gly

470 475 480 485

tac gct gag gtt gaa acc cgc ggt gga ttc ttc gga cct gat cca gaa 1603

Tyr Ala Glu Val Glu Thr Arg Gly Gly Phe Phe Gly Pro Asp Pro Glu

490 495 500

gac atc cgt gcg aag gct aag gaa att gag cac gca aac cac att gag 1651

Asp Ile Arg Ala Lys Ala Lys Glu Ile Glu His Ala Asn His Ile Glu

505 510 515

gaa gcg aac act ctt cgt gca ctc aac gag gca aac att gag cgt gac 1699

Glu Ala Asn Thr Leu Arg Ala Leu Asn Glu Ala Asn Ile Glu Arg Asp

520 525 530

aag aat gag ggc aag aac tagtttctag gacttcatct ctgaaactcc 1747

Lys Asn Glu Gly Lys Asn

535

<210>54

<211>539

<212>PRT

<213>谷氨酸棒状杆菌

<400>54

Met Ser Leu Ala Thr Val Gly Asn Asn Leu Asp Ser Arg Tyr Thr Met

1 5 10 15

Ala Ser Gly Ile Arg Arg Gln Ile Asn Lys Val Phe Pro Thr His Trp

20 25 30

Ser Phe Met Leu Gly Glu Ile Ala Leu Tyr Ser Phe Ile Val Leu Leu

35 40 45

Leu Thr Gly Val Tyr Leu Thr Leu Phe Phe Asp Pro Ser Ile Thr Lys

50 55 60

Val Ile Tyr Asp Gly Gly Tyr Leu Pro Leu Asn Gly Val Glu Met Ser

65 70 75 80

Arg Ala Tyr Ala Thr Ala Leu Asp Ile Ser Phe Glu Val Arg Gly Gly

85 90 95

Leu Phe Ile Arg Gln Met His His Trp Ala Ala Leu Leu Phe Val Val

100 105 110

Ser Met Leu Val His Met Leu Arg Ile Phe Phe Thr Gly Ala Phe Arg

115 120 125

Arg Pro Arg Glu Ala Asn Trp Ile Ile Gly Val Val Leu Ile Ile Leu

130 135 140

Gly Met Ala Glu Gly Phe Met Gly Tyr Ser Leu Pro Asp Asp Leu Leu

145 150 155 160

Ser Gly Val Gly Leu Arg Ile Met Ser Ala Ile Ile Val Gly Leu Pro

165 170 175

Ile Ile Gly Thr Trp Met His Trp Leu Ile Phe Gly Gly Asp Phe Pro

180 185 190

Ser Asp Leu Met Leu Asp Arg Phe Tyr Ile Ala His Val Leu Ile Ile

195 200 205

Pro Ala Ile Leu Leu Gly Leu Ile Ala Ala His Leu Ala Leu Val Trp

210 215 220

Tyr Gln Lys His Thr Gln Phe Pro Gly Ala Gly Arg Thr Glu Asn Asn

225 230 235 240

Val Ile Gly Ile Arg Ile Met Pro Leu Phe Ala Val Lys Ala Val Ala

245 250 255

Phe Gly Leu Ile Val Phe Gly Phe Leu Ala Leu Leu Ala Gly Val Thr

260 265 270

Thr Ile Asn Ala Ile Trp Asn Leu Gly Pro Tyr Asn Pro Ser Gln Val

275 280 285

Ser Ala Gly Ser Gln Pro Asp Val Tyr Met Leu Trp Thr Asp Gly Ala

290 295 300

Ala Arg Val Met Pro Ala Trp Glu Leu Tyr Leu Gly Asn Tyr Thr Ile

305 310 315 320

Pro Ala Val Phe Trp Val Ala Val Met Leu Gly Ile Leu Val Val Leu

325 330 335

Leu Val Thr Tyr Pro Phe Ile Glu Arg Lys Phe Thr Gly Asp Asp Ala

340 345 350

His His Asn Leu Leu Gln Arg Pro Arg Asp Val Pro Val Arg Thr Ser

355 360 365

Leu Gly Val Met Ala Leu Val Phe Tyr Ile Leu Leu Thr Val Ser Gly

370 375 380

Gly Asn Asp Val Tyr Ala Met Gln Phe His Val Ser Leu Asn Ala Met

385 390 395 400

Thr Trp Ile Gly Arg Ile Gly Leu Ile Val Gly Pro Ala Ile Ala Tyr

405 410 415

Phe Ile Thr Tyr Arg Leu Cys Ile Gly Leu Gln Arg Ser Asp Arg Glu

420 425 430

Val Leu Glu His Gly Ile Glu Thr Gly Ile Ile Lys Gln Met Pro Asn

435 440 445

Gly Ala Phe Ile Glu Val His Gln Pro Leu Gly Pro Val Asp Asp His

450 455 460

Gly His Pro Ile Pro Leu Pro Tyr Ala Gly Ala Ala Val Pro Lys Gln

465 470 475 480

Met Asn Gln Leu Gly Tyr Ala Glu Val Glu Thr Arg Gly Gly Phe Phe

485 490 495

Gly Pro Asp Pro Glu Asp Ile Arg Ala Lys Ala Lys Glu Ile Glu His

500 505 510

Ala Asn His Ile Glu Glu Ala Asn Thr Leu Arg Ala Leu Asn Glu Ala

515 520 525

Asn Ile Glu Arg Asp Lys Asn Glu Gly Lys Asn

530 535

<210>55

<211>448

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(418)

<223>RXA02236

<400>55

gcaggctgac atccttggta ttaaccaggt gtaccctcga tttctggata ctttggtatt 60

ccttttgtca ctaaaaacca cacgataacg gaggaacccc gtg gcc ctt cca cag 115

Val Ala Leu Pro Gln

1 5

ttg act gat gag cag cgc aag gca gcg ctt gct aag gca gca gag gca 163

Leu Thr Asp Glu Gln Arg Lys Ala Ala Leu Ala Lys Ala Ala Glu Ala

10 15 20

cgc aag gca cgc gca gag ctc aaa gag aac ctg aag cgc ggc aac act 211

Arg Lys Ala Arg Ala Glu Leu Lys Glu Asn Leu Lys Arg Gly Asn Thr

25 30 35

aac ctc agg gaa gtt ctg gac aag gct gag tct gac gag atc atc ggc 259

Asn Leu Arg Glu Val Leu Asp Lys Ala Glu Ser Asp Glu Ile Ile Gly

40 45 50

aag acc aag gtc tcc gct ctc ctc gag gct ctc cct aag gtt ggc aag 307

Lys Thr Lys Val Ser Ala Leu Leu Glu Ala Leu Pro Lys Val Gly Lys

55 60 65

gtc aag gca aag gag att atg gac gag ctg ggc att gct cag acc cgt 355

Val Lys Ala Lys Glu Ile Met Asp Glu Leu Gly Ile Ala Gln Thr Arg

70 75 80 85

cgt ctt cgt gga ctg ggt gac cgt cag cgt cgc gca ctt ctc gag cgt 403

Arg Leu Arg Gly Leu Gly Asp Arg Gln Arg Arg Ala Leu Leu Glu Arg

90 95 100

ttc ggc ttc gag gat taattcttca gtgtcgggcg ataaccaact 448

Phe Gly Phe Glu Asp

105

<210>56

<211>106

<212>PRT

<213>谷氨酸棒状杆菌

<400>56

Val Ala Leu Pro Gln Leu Thr Asp Glu Gln Arg Lys Ala Ala Leu Ala

1 5 10 15

Lys Ala Ala Glu Ala Arg Lys Ala Arg Ala Glu Leu Lys Glu Asn Leu

20 25 30

Lys Arg Gly Asn Thr Asn Leu Arg Glu Val Leu Asp Lys Ala Glu Ser

35 40 45

Asp Glu Ile Ile Gly Lys Thr Lys Val Ser Ala Leu Leu Glu Ala Leu

50 55 60

Pro Lys Val Gly Lys Val Lys Ala Lys Glu Ile Met Asp Glu Leu Gly

65 70 75 80

Ile Ala Gln Thr Arg Arg Leu Arg Gly Leu Gly Asp Arg Gln Arg Arg

85 90 95

Ala Leu Leu Glu Arg Phe Gly Phe Glu Asp

100 105

<210>57

<211>1003

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(973)

<223>RXA02267

<400>57

tgcgctcggc aagtgttttg cttatcgacg tctccccaca taacaatccc aactcgaagc 60

accaacgatt caagccttat cagttttgta caggaaaata gtg caa aaa tgg ggt 115

Val Gln Lys Trp Gly

1 5

tta agc ttc gtg gag agg att gtc atc atg aac aac gtg caa cag ttt 163

Leu Ser Phe Val Glu Arg Ile Val Ile Met Asn Asn Val Gln Gln Phe

10 15 20

cat cga ttt ttt gat gat tcc gca gtc tat tat ccc tgc ttc gtc ccg 211

His Arg Phe Phe Asp Asp Ser Ala Val Tyr Tyr Pro Cys Phe Val Pro

25 30 35

ctt gac cga gcc atc ggc gaa cac ttt gat cgt cag aac aaa ccg atg 259

Leu Asp Arg Ala Ile Gly Glu His Phe Asp Arg Gln Asn Lys Pro Met

40 45 50

tcc aga ttc atc gga acg ctc att ctg ccg tta gcc aaa ctg gaa gaa 307

Ser Arg Phe Ile Gly Thr Leu Ile Leu Pro Leu Ala Lys Leu Glu Glu

55 60 65

gcc gcc caa tac acc ggc gat gaa gtc ctt cgc gtg tcg gca gta atc 355

Ala Ala Gln Tyr Thr Gly Asp Glu Val Leu Arg Val Ser Ala Val Ile

70 75 80 85

agt act gat ggg ctc gct gat ctg cga agg gat ttt tac gaa ctc ccc 403

Ser Thr Asp Gly Leu Ala Asp Leu Arg Arg Asp Phe Tyr Glu Leu Pro

90 95 100

aac atc gac atc gcc tcg gtg gaa atc aag ctg gtc ggc gca gcc ctc 451

Asn Ile Asp Ile Ala Ser Val Glu Ile Lys Leu Val Gly Ala Ala Leu

105 110 115

acc aac acc gct tgg ttg gga gat gtg gaa aaa ctc atc caa caa cat 499

Thr Asn Thr Ala Trp Leu Gly Asp Val Glu Lys Leu Ile Gln Gln His

120 125 130

cgc aac act ttc gta tgg gtt gag att ccg aca gcc ctg gtc acc gca 547

Arg Asn Thr Phe Val Trp Val Glu Ile Pro Thr Ala Leu Val Thr Ala

135 140 145

gat att gtc cga aaa ctc cgc cac atg gga gct ggc ctg aaa tac aga 595

Asp Ile Val Arg Lys Leu Arg His Met Gly Ala Gly Leu Lys Tyr Arg

150 155 160 165

act gga ggt gat agg gaa gag ctc ttc ccc tca ccg cag gac ttg gtc 643

Thr Gly Gly Asp Arg Glu Glu Leu Phe Pro Ser Pro Gln Asp Leu Val

170 175 180

act gtg ctg cgc acc gcc atc gat gct gca ttg ccg ttt aaa ctc act 691

Thr Val Leu Arg Thr Ala Ile Asp Ala Ala Leu Pro Phe Lys Leu Thr

185 190 195

gca ggc ctg cat cgt gct ctc agg tat cgt gac gag aaa acc ggc cga 739

Ala Gly Leu His Arg Ala Leu Arg Tyr Arg Asp Glu Lys Thr Gly Arg

200 205 210

ctt cac ttc gga ttc ctc aac att gca gcc gcc gtg gcg aca ctt cgt 787

Leu His Phe Gly Phe Leu Asn Ile Ala Ala Ala Val Ala Thr Leu Arg

215 220 225

gct gga aaa ggc gag gca gag gca ctg aag atc ctt gaa ggc gat gat 835

Ala Gly Lys Gly Glu Ala Glu Ala Leu Lys Ile Leu Glu Gly Asp Asp

230 235 240 245

gcc gct ccg ctt att cac gca cta caa agc ggc gaa aac tgg cgg gat 883

Ala Ala Pro Leu Ile His Ala Leu Gln Ser Gly Glu Asn Trp Arg Asp

250 255 260

tcc ttc cgc agc ttc agt acc tgc aat gtt gtt gaa cca ctc aac act 931

Ser Phe Arg Ser Phe Ser Thr Cys Asn Val Val Glu Pro Leu Asn Thr

265 270 275

ctg att gat ctt gat gtg ttg gcg gaa gga gac gta cat ccc 973

Leu Ile Asp Leu Asp Val Leu Ala Glu Gly Asp Val His Pro

280 285 290

taaggatcga cgctagttag atcggttttt 1003

<210>58

<211>291

<212>PRT

<213>谷氨酸棒状杆菌

<400>58

Val Gln Lys Trp Gly Leu Ser Phe Val Glu Arg Ile Val Ile Met Asn

1 5 10 15

Asn Val Gln Gln Phe His Arg Phe Phe Asp Asp Ser Ala Val Tyr Tyr

20 25 30

Pro Cys Phe Val Pro Leu Asp Arg Ala Ile Gly Glu His Phe Asp Arg

35 40 45

Gln Asr Lys Pro Met Ser Arg Phe Ile Gly Thr Leu Ile Leu Pro Leu

50 55 60

Ala Lys Leu Glu Glu Ala Ala Gln Tyr Thr Gly Asp Glu Val Leu Arg

65 70 75 80

Val Ser Ala Val Ile Ser Thr Asp Gly Leu Ala Asp Leu Arg Arg Asp

85 90 95

Phe Tyr Glu Leu Pro Asn Ile Asp Ile Ala Ser Val Glu Ile Lys Leu

100 105 110

Val Gly Ala Ala Leu Thr Asn Thr Ala Trp Leu Gly Asp Val Glu Lys

115 120 125

Leu Ile Gln Gln His Arg Asn Thr Phe Val Trp Val Glu Ile Pro Thr

130 135 140

Ala Leu Val Thr Ala Asp Ile Val Arg Lys Leu Arg His Met Gly Ala

145 150 155 160

Gly Leu Lys Tyr Arg Thr Gly Gly Asp Arg Glu Glu Leu Phe Pro Ser

165 170 175

Pro Gln Asp Leu Val Thr Val Leu Arg Thr Ala Ile Asp Ala Ala Leu

180 185 190

Pro Phe Lys Leu Thr Ala Gly Leu His Arg Ala Leu Arg Tyr Arg Asp

195 200 205

Glu Lys Thr Gly Arg Leu His Phe Gly Phe Leu Asn Ile Ala Ala Ala

210 215 220

Val Ala Thr Leu Arg Ala Gly Lys Gly Glu Ala Glu Ala Leu Lys Ile

225 230 235 240

Leu Glu Gly Asp Asp Ala Ala Pro Leu Ile His Ala Leu Gln Ser Gly

245 250 255

Glu Asn Trp Arg Asp Ser Phe Arg Ser Phe Ser Thr Cys Asn Val Val

260 265 270

Glu Pro Leu Asn Thr Leu Ile Asp Leu Asp Val Leu Ala Glu Gly Asp

275 280 285

Val His Pro

290

<210>59

<211>1984

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1954)

<223>RXA02280

<400>59

ggtcgaggtg tcgtagatgt caatgagctt cgcgattgcg tcatcgatcg ttgttgcttc 60

catgcgcacc acactatctt tctgcacgcc ctgatgccct gtg gat tca aaa ctg 115

Val Asp Ser Lys Leu

1 5

tgc ttt tat agg cgt atg caa gaa tcc tca cgt gat aat ttc caa gtt 163

Cys Phe Tyr Arg Arg Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val

10 15 20

gac ctc ggc ggc gtt gtt gat ctt ttg agt cgc cac att tat tcc ggt 211

Asp Leu Gly Gly Val Val Asp Leu Leu Ser Arg His Ile Tyr Ser Gly

25 30 35

ccg agg gtg tat gtg cgt gag ttg ctg cag aat gcg gtt gat gct tgt 259

Pro Arg Val Tyr Val Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys

40 45 50

act gca cgt tct gaa cag ggt gag gag ggc tac gag ccg agt att cgt 307

Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly Tyr Glu Pro Ser Ile Arg

55 60 65

att cgg ccg gtg acc aag gat cgt gcc acg ttt tca ctg gtt gat aat 355

Ile Arg Pro Val Thr Lys Asp Arg Ala Thr Phe Ser Leu Val Asp Asn

70 75 80 85

ggt acg ggc ctg acc gcg cag gag gcg cgg gaa ttg ctg gcg acg gtg 403

Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg Glu Leu Leu Ala Thr Val

90 95 100

ggg cgg acg tcg aaa cgc gat gaa ttc ggt ctg cag cgg gaa ggt cgc 451

Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg

105 110 115

ctg ggg caa ttt ggc atc ggg ctg ctt agt tgt ttc atg gtg gcg gat 499

Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser Cys Phe Met Val Ala Asp

120 125 130

gag atc acc atg gtg tcg cat gcg gag ggt gcg tcg gcg att cgg tgg 547

Glu Ile Thr Met Val Ser His Ala Glu Gly Ala Ser Ala Ile Arg Trp

135 140 145

act ggt cat gcg gat ggc acc ttt aac ctg gag att ctt ggg gat gac 595

Thr Gly His Ala Asp Gly Thr Phe Asn Leu Glu Ile Leu Gly Asp Asp

150 155 160 165

gca acg gat gtc att ccg gtg ggc acg act gtg cac ctg act ccg cgc 643

Ala Thr Asp Val Ile Pro Val Gly Thr Thr Val His Leu Thr Pro Arg

170 175 180

cct gat gag cgc acg ttg ctg acg gaa aat tcc gtg gtc acc att gct 691

Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn Ser Val Val Thr Ile Ala

185 190 195

agt aat tat ggc cgc tac ctg ccg att cct att gtg gtg cag ggt gag 739

Ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro Ile Val Val Gln Gly Glu

200 205 210

aaa aac acc acc atc act aca tcg ccg gtg ttt gca aag gat act gat 787

Lys Asn Thr Thr Ile Thr Thr Ser Pro Val Phe Ala Lys Asp Thr Asp

215 220 225

cag cag cac agg ctg tat gcc ggc cgg gag cgc ctt ggt aaa act cct 835

Gln Gln His Arg Leu Tyr Ala Gly Arg Glu Arg Leu Gly Lys Thr Pro

230 235 240 245

ttt gat gtc atc gat ctc acc ggt cct ggc atc gag ggt gtg gct tat 883

Phe Asp Val Ile Asp Leu Thr Gly Pro Gly Ile Glu Gly Val Ala Tyr

250 255 260

gta ttg ccg gag gcc cag gct ccg cat atg tcc agg cgt cac agt att 931

Val Leu Pro Glu Ala Gln Ala Pro His Met Ser Arg Arg His Ser Ile

265 270 275

tat gtc aac cgc atg ttg gtc tct gat ggg cct tcc acg gtg ctg ccc 979

Tyr Val Asn Arg Met Leu Val Ser Asp Gly Pro Ser Thr Val Leu Pro

280 285 290

aac tgg gcg ttc ttt gtg gaa tgt gaa atc aat tca acc gat ttg gaa 1027

Asn Trp Ala Phe Phe Val Glu Cys Glu Ile Asn Ser Thr Asp Leu Glu

295 300 305

ccc acc gca tcg cgt gaa gcg ctc atg gat gac acc gcg ttc gcg gca 1075

Pro Thr Ala Ser Arg Glu Ala Leu Met Asp Asp Thr Ala Phe Ala Ala

310 315 320 325

acc agg gaa cat atc ggt gag tgc att aaa tcg tgg ctg att aat ctc 1123

Thr Arg Glu His Ile Gly Glu Cys Ile Lys Ser Trp Leu Ile Asn Leu

330 335 340

gcc atg acc aag cct cac cgc gtg cgg gaa ttt act gcg att cat gat 1171

Ala Met Thr Lys Pro His Arg Val Arg Glu Phe Thr Ala Ile His Asp

345 350 355

ctt gcc ctg cgc gag ctg tgc caa tcg gac gcg gac ctg gct gaa acc 1219

Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp Ala Asp Leu Ala Glu Thr

360 365 370

atg ttg ggt ctt ctc acc ttg gag acc tcc cgt ggt cgc atc tcg atc 1267

Met Leu Gly Leu Leu Thr Leu Glu Thr Ser Arg Gly Arg Ile Ser Ile

375 380 385

ggt gag atc acc acg ttg tcc atc acc gag gat gtg tcg ctg cag ctg 1315

Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu Asp Val Ser Leu Gln Leu

390 395 400 405

gct acc acg ttg gat gat ttc agg cag ctc aac acc att gcg cgc ccg 1363

Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu Asn Thr Ile Ala Arg Pro

410 415 420

gac acc ttg att att aat ggc ggc tac att cac gac agc gat ctg gct 1411

Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile His Asp Ser Asp Leu Ala

425 430 435

cgg ctc att ccc gtt cac tac cca ccg ctt acg gta tct act gct gac 1459

Arg Leu Ile Pro Val His Tyr Pro Pro Leu Thr Val Ser Thr Ala Asp

440 445 450

ctg cgc gaa tcc atg gat ctg atg gag ctt ccg ccg ctg cag gac att 1507

Leu Arg Glu Ser Met Asp Leu Met Glu Leu Pro Pro Leu Gln Asp Ile

455 460 465

gag aaa gcc aag gca ctg gat gcg cag gtc acg gaa tca ttg aag gat 1555

Glu Lys Ala Lys Ala Leu Asp Ala Gln Val Thr Glu Ser Leu Lys Asp

470 475 480 485

ttt cag atc aag ggc gca acg agg gtt ttt gaa ccc gca gat gtt cct 1603

Phe Gln Ile Lys Gly Ala Thr Arg Val Phe Glu Pro Ala Asp Val Pro

490 495 500

gcc gtg gtg atc att gat tcc aag gcg cag gcc tca cgg gat cgc aat 1651

Ala Val Val Ile Ile Asp Ser Lys Ala Gln Ala Ser Arg Asp Arg Asn

505 510 515

gaa aca caa agc gca acc act gat cgt tgg gct gac att ttg gca acg 1699

Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp Ala Asp Ile Leu Ala Thr

520 525 530

gtg gat aac acg ttg agc cgt caa aca gcc aac att cca cag gat cag 1747

Val Asp Asn Thr Leu Ser Arg Gln Thr Ala Asn Ile Pro Gln Asp Gln

535 540 545

gga ctg tcg gcg ttg tgc ttg aat tgg aac aat tcg ctg gtc agg aaa 1795

Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn Asn Ser Leu Val Arg Lys

550 555 560 565

ttg gcg tcc act gat gac acc gcc gtg gtg tcg cgc acg gtg cgt ttg 1843

Leu Ala Ser Thr Asp Asp Thr Ala Val Val Ser Arg Thr Val Arg Leu

570 575 580

ctc tac gtt cag gca ttg ttg tcc agc aag agg cca ctg cgg gtg aag 1891

Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys Arg Pro Leu Arg Val Lys

585 590 595

gaa cgc gcg ctg ctt aat gat tcg ctg gca gat ctg gtt tct ttg tct 1939

Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala Asp Leu Val Ser Leu Ser

600 605 610

ttg tca tcc gat atc taagacaatc ctccgctaat ctcgagggca 1984

Leu Ser Ser Asp Ile

615

<210>60

<211>618

<212>PRT

<213>谷氨酸棒状杆菌

<400>60

Val Asp Ser Lys Leu Cys Phe Tyr Arg Arg Met Gln Glu Ser Ser Arg

1 5 10 15

Asp Asn Phe Gln Val Asp Leu Gly Gly Val Val Asp Leu Leu Ser Arg

20 25 30

His Ile Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln Asn

35 40 45

Ala Val Asp Ala Cys Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly Tyr

50 55 60

Glu Pro Ser Ile Arg Ile Arg Pro Val Thr Lys Asp Arg Ala Thr Phe

65 70 75 80

Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg Glu

85 90 95

Leu Leu Ala Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly Leu

100 105 110

Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser Cys

115 120 125

Phe Met Val Ala Asp Glu Ile Thr Met Val Ser His Ala Glu Gly Ala

130 135 140

Ser Ala Ile Arg Trp Thr Gly His Ala Asp Gly Thr Phe Asn Leu Glu

145 150 155 160

Ile Leu Gly Asp Asp Ala Thr Asp Val Ile Pro Val Gly Thr Thr Val

165 170 175

His Leu Thr Pro Arg Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn Ser

180 185 190

Val Val Thr Ile Ala ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro Ile

195 200 205

Val Val Gln Gly Glu Lys Asn Thr Thr Ile Thr Thr Ser Pro Val Phe

210 215 220

Ala Lys Asp Thr Asp Gln Gln His Arg Leu Tyr Ala Gly Arg Glu Arg

225 230 235 240

Leu Gly Lys Thr Pro Phe Asp Val Ile Asp Leu Thr Gly Pro Gly Ile

245 250 255

Glu Gly Val Ala Tyr Val Leu Pro Glu Ala Gln Ala Pro His Met Ser

260 265 270

Arg Arg His Ser Ile Tyr Val Asn Arg Met Leu Val Ser Asp Gly Pro

275 280 285

Ser Thr Val Leu Pro Asn Trp Ala Phe Phe Val Glu Cys Glu Ile Asn

290 295 300

Ser Thr Asp Leu Glu Pro Thr Ala Ser Arg Glu Ala Leu Met Asp Asp

305 310 315 320

Thr Ala Phe Ala Ala Thr Arg Glu His Ile Gly Glu Cys Ile Lys Ser

325 330 335

Trp Leu Ile Asn Leu Ala Met Thr Lys Pro His Arg Val Arg Glu Phe

340 345 350

Thr Ala Ile His Asp Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp Ala

355 360 365

Asp Leu Ala Glu Thr Met Leu Gly Leu Leu Thr Leu Glu Thr Ser Arg

370 375 380

Gly Arg Ile Ser Ile Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu Asp

385 390 395 400

Val Ser Leu Gln Leu Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu Asn

405 410 415

Thr Ile Ala Arg Pro Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile His

420 425 430

Asp Ser Asp Leu Ala Arg Leu Ile Pro Val His Tyr Pro Pro Leu Thr

435 440 445

Val Ser Thr Ala Asp Leu Arg Glu Ser Met Asp Leu Met Glu Leu Pro

450 455 460

Pro Leu Gln Asp Ile Glu Lys Ala Lys Ala Leu Asp Ala Gln Val Thr

465 470 475 480

Glu Ser Leu Lys Asp Phe Gln Ile Lys Gly Ala Thr Arg Val Phe Glu

485 490 495

Pro Ala Asp Val Pro Ala Val Val Ile Ile Asp Ser Lys Ala Gln Ala

500 505 510

Ser Arg Asp Arg Asn Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp Ala

515 520 525

Asp Ile Leu Ala Thr Val Asp Asn Thr Leu Ser Arg Gln Thr Ala Asn

530 535 540

Ile Pro Gln Asp Gln Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn Asn

545 550 555 560

Ser Leu Val Arg Lys Leu Ala Ser Thr Asp Asp Thr Ala Val Val Ser

565 570 575

Arg Thr Val Arg Leu Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys Arg

580 585 590

Pro Leu Arg Val Lys Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala Asp

595 600 605

Leu Val Ser Leu Ser Leu Ser Ser Asp Ile

610 615

<210>61

<211>1792

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1762)

<223>RXA02388

<400>61

ttcgggagag caacggtggg tttagcaccg tggaggattt actgcaggtc aaggggattg 60

ggccctcaaa gtttgagcag atctctggat tggtgtcccc atg att gag gtg cgt 115

Met Ile Glu Val Arg

1 5

ttg gtt ccg gtg gcg gct gtg atg tgg atg gct gtc gct gcg ttg att 163

Leu Val Pro Val Ala Ala Val Met Trp Met Ala Val Ala Ala Leu Ile

10 15 20

atc agt ggt tcg tgg gtg ttg tcg gtg ggg att gtt ggc atc gcg atc 211

Ile Ser Gly Ser Trp Val Leu Ser Val Gly Ile Val Gly Ile Ala Ile

25 30 35

att gct gct tgt gtg ttt aaa cac tgg ggt caa gct gtg gtg ata gct 259

Ile Ala Ala Cys Val Phe Lys His Trp Gly Gln Ala Val Val Ile Ala

40 45 50

gca ctg ggc gtt ggt gcc gta gtg atg gct gcg ttg aga atc agc agc 307

Ala Leu Gly Val Gly Ala Val Val Met Ala Ala Leu Arg Ile Ser Ser

55 60 65

gcg aag gca ttt gaa gca ccg caa acc tgg gtg ggt acc gca gaa acc 355

Ala Lys Ala Phe Glu Ala Pro Gln Thr Trp Val Gly Thr Ala Glu Thr

70 75 80 85

atc aag ttt tta gac agc ggt gat caa cta atc ggt ttg aga gta gaa 403

Ile Lys Phe Leu Asp Ser Gly Asp Gln Leu Ile Gly Leu Arg Val Glu

90 95 100

ggc tat cca gcg ccg att cca gtg ttt tac tct ggt agc gac acc att 451

Gly Tyr Pro Ala Pro Ile Pro Val Phe Tyr Ser Gly Ser Asp Thr Ile

105 110 115

gag aaa gcc tct ctc att gca gtg tcc ggt cgg att aaa cca gat agt 499

Glu Lys Ala Ser Leu Ile Ala Val Ser Gly Arg Ile Lys Pro Asp Ser

120 125 130

ttc cct ggg gtg ggt gat ctg acc att tcc act gaa gac att gat cag 547

Phe Pro Gly Val Gly Asp Leu Thr Ile Ser Thr Glu Asp Ile Asp Gln

135 140 145

ttg gaa ccg acc act ggt tat agc gca tgg gtg aac cag gtg cgt gac 595

Leu Glu Pro Thr Thr Gly Tyr Ser Ala Trp Val Asn Gln Val Arg Asp

150 155 160 165

ggg ttt tcc caa gcc gtg gaa gaa acc gtg ggg gag tct tcc cgt gga 643

Gly Phe Ser Gln Ala Val Glu Glu Thr Val Gly Glu Ser Ser Arg Gly

170 175 180

ctg att cca ggc atg gtg ttg ggg gat acg cgg ttg cag ggg tca att 691

Leu Ile Pro Gly Met Val Leu Gly Asp Thr Arg Leu Gln Gly Ser Ile

185 190 195

gaa gcc caa acc tat att gat acg ggg ttg tct cac ctg tca gct gtt 739

Glu Ala Gln Thr Tyr Ile Asp Thr Gly Leu Ser His Leu Ser Ala Val

200 205 210

agt gga agc aat gta gcc att gtg gtg tcc tct gtg gtg gtg ttg tcg 787

Ser Gly Ser Asn Val Ala Ile Val Val Ser Ser Val Val Val Leu Ser

215 220 225

tat ttt ctc acc gct ggg cca cgc atc agg gtg gtg gcg tca ttg ctg 835

Tyr Phe Leu Thr Ala Gly Pro Arg Ile Arg Val Val Ala Ser Leu Leu

230 235 240 245

tcc tta gtt att ttt gtc tcc ctc gtg ggg ttt gaa cca agt gtg ctt 883

Ser Leu Val Ile Phe Val Ser Leu Val Gly Phe Glu Pro Ser Val Leu

250 255 260

cgt gct tcg gtc aca ggc atc gtg ggg ctt ctg gca atc atc aac tct 931

Arg Ala Ser Val Thr Gly Ile Val Gly Leu Leu Ala Ile Ile Asn Ser

265 270 275

tct cgg atg gag ccg atg cat ggg ttg agt ctt tcg gtg att tgc tta 979

Ser Arg Met Glu Pro Met His Gly Leu Ser Leu Ser Val Ile Cys Leu

280 285 290

ctg ttt tat gat tcc aac ctg gcg gtg cat tac gga ttc tta ctc tcg 1027

Leu Phe Tyr Asp Ser Asn Leu Ala Val His Tyr Gly Phe Leu Leu Ser

295 300 305

tgt gca gca act gct ggc att gtg atg ctt caa cca ctg ctg tac cgt 1075

Cys Ala Ala Thr Ala Gly Ile Val Met Leu Gln Pro Leu Leu Tyr Arg

310 315 320 325

gcc atc ggt cca cca ctg gcg gtg tgg aaa gta cca gac atc gtg gtg 1123

Ala Ile Gly Pro Pro Leu Ala Val Trp Lys Val Pro Asp Ile Val Val

330 335 340

cgc gct ttc gcg gtg tcc att gcc gct gat ctg gtg acc atc ccg att 1171

Arg Ala Phe Ala Val Ser Ile Ala Ala Asp Leu Val Thr Ile Pro Ile

345 350 355

atc gct ctg atg gct cgc caa ata tcc ctc gtg gca gtg ctg gcc aac 1219

Ile Ala Leu Met Ala Arg Gln Ile Ser Leu Val Ala Val Leu Ala Asn

360 365 370

gtg ttg gtt gaa tta gct gtt cca ccc atc acg ttg ctt ggg ttg att 1267

Val Leu Val Glu Leu Ala Val Pro Pro Ile Thr Leu Leu Gly Leu Ile

375 380 385

gcc gtg ctg gca agc ctt ctt ccc tgg cca gtg gaa tac cca ctc ttg 1315

Ala Val Leu Ala Ser Leu Leu Pro Trp Pro Val Glu Tyr Pro Leu Leu

390 395 400 405

aaa atc att gag ccc ttc acc tgg tgg att cat cac gtg gcc aag tgg 1363

Lys Ile Ile Glu Pro Phe Thr Trp Trp Ile His His Val Ala Lys Trp

410 415 420

tgc caa caa tta ccc aat tcg acg ctg gaa ata agt gct ggt tgg gca 1411

Cys Gln Gln Leu Pro Asn Ser Thr Leu Glu Ile Ser Ala Gly Trp Ala

425 430 435

ggg att gcc tgg gcg tgt atg gca gcg gtg tgg gtg gtg gtg att atc 1459

Gly Ile Ala Trp Ala Cys Met Ala Ala Val Trp Val Val Val Ile Ile

440 445 450

tac aaa gga tat gtg cgc acc ctt gca gtg tgt tgt gtc tgc ttc ttt 1507

Tyr Lys Gly Tyr Val Arg Thr Leu Ala Val Cys Cys Val Cys Phe Phe

455 460 465

ctt ttc ggc gcg tgg aat aac aga ctg cca gcc caa ata gat ccg aca 1555

Leu Phe Gly Ala Trp Asn Asn Arg Leu Pro Ala Gln Ile Asp Pro Thr

470 475 480 485

gag ctg cgg ttt gtc atc atc gcc gat gat tct gag ctc act gat gtg 1603

Glu Leu Arg Phe Val Ile Ile Ala Asp Asp Ser Glu Leu Thr Asp Val

490 495 500

ccc gaa cat gca gaa ttg atc atc gtg gaa gac ccc cac ggc agc atg 1651

Pro Glu His Ala Glu Leu Ile Ile Val Glu Asp Pro His Gly Ser Met

505 510 515

tcc gat cgc ccc atc gtc acc aga gaa gga atc cct gtg ctg tat cca 1699

Ser Asp Arg Pro Ile Val Thr Arg Glu Gly Ile Pro Val Leu Tyr Pro

520 525 530

tac cgc gat ggg gag gtc agc ctt cat att gat ggc acc cag cat gca 1747

Tyr Arg Asp Gly Glu Val Ser Leu His Ile Asp Gly Thr Gln His Ala

535 540 545

gcg gac ggg aga ttt taacgacact tgtggcacga tggtcacgtg 1792

Ala Asp Gly Arg Phe

550

<210>62

<211>554

<212>PRT

<213>谷氨酸棒状杆菌

<400>62

Met Ile Glu Val Arg Leu Val Pro Val Ala Ala Val Met Trp Met Ala

1 5 10 15

Val Ala Ala Leu Ile Ile Ser Gly Ser Trp Val Leu Ser Val Gly Ile

20 25 30

Val Gly Ile Ala Ile Ile Ala Ala Cys Val Phe Lys His Trp Gly Gln

35 40 45

Ala Val Val Ile Ala Ala Leu Gly Val Gly Ala Val Val Met Ala Ala

50 55 60

Leu Arg Ile Ser Ser Ala Lys Ala Phe Glu Ala Pro Gln Thr Trp Val

65 70 75 80

Gly Thr Ala Glu Thr Ile Lys Phe Leu Asp Ser Gly Asp Gln Leu Ile

85 90 95

Gly Leu Arg Val Glu Gly Tyr Pro Ala Pro Ile Pro Val Phe Tyr Ser

100 105 110

Gly Ser Asp Thr Ile Glu Lys Ala Ser Leu Ile Ala Val Ser Gly Arg

115 120 125

Ile Lys Pro Asp Ser Phe Pro Gly Val Gly Asp Leu Thr Ile Ser Thr

130 135 140

Glu Asp Ile Asp Gln Leu Glu Pro Thr Thr Gly Tyr Ser Ala Trp Val

145 150 155 160

Asn Gln Val Arg Asp Gly Phe Ser Gln Ala Val Glu Glu Thr Val Gly

165 170 175

Glu Ser Ser Arg Gly Leu Ile Pro Gly Met Val Leu Gly Asp Thr Arg

180 185 190

Leu Gln Gly Ser Ile Glu Ala Gln Thr Tyr Ile Asp Thr Gly Leu Ser

195 200 205

His Leu Ser Ala Val Ser Gly Ser Asn Val Ala Ile Val Val Ser Ser

210 215 220

Val Val Val Leu Ser Tyr Phe Leu Thr Ala Gly Pro Arg Ile Arg Val

225 230 235 240

Val Ala Ser Leu Leu Ser Leu Val Ile Phe Val Ser Leu Val Gly Phe

245 250 255

Glu Pro Ser Val Leu Arg Ala Ser Val Thr Gly Ile Val Gly Leu Leu

260 265 270

Ala Ile Ile Asn Ser Ser Arg Met Glu Pro Met His Gly Leu Ser Leu

275 280 285

Ser Val Ile Cys Leu Leu Phe Tyr Asp Ser Asn Leu Ala Val His Tyr

290 295 300

Gly Phe Leu Leu Ser Cys Ala Ala Thr Ala Gly Ile Val Met Leu Gln

305 310 315 320

Pro Leu Leu Tyr Arg Ala Ile Gly Pro Pro Leu Ala Val Trp Lys Val

325 330 335

Pro Asp Ile Val Val Arg Ala Phe Ala Val Ser Ile Ala Ala Asp Leu

340 345 350

Val Thr Ile Pro Ile Ile Ala Leu Met Ala Arg Gln Ile Ser Leu Val

355 360 365

Ala Val Leu Ala Asn Val Leu Val Glu Leu Ala Val Pro Pro Ile Thr

370 375 380

Leu Leu Gly Leu Ile Ala Val Leu Ala Ser Leu Leu Pro Trp Pro Val

385 390 395 400

Glu Tyr Pro Leu Leu Lys Ile Ile Glu Pro Phe Thr Trp Trp Ile His

405 410 415

His Val Ala Lys Trp Cys Gln Gln Leu Pro Asn Ser Thr Leu Glu Ile

420 425 430

Ser Ala Gly Trp Ala Gly Ile Ala Trp Ala Cys Met Ala Ala Val Trp

435 440 445

Val Val Val Ile Ile Tyr Lys Gly Tyr Val Arg Thr Leu Ala Val Cys

450 455 460

Cys Val Cys Phe Phe Leu Phe Gly Ala Trp Asn Asn Arg Leu Pro Ala

465 470 475 480

Gln Ile Asp Pro Thr Glu Leu Arg Phe Val Ile Ile Ala Asp Asp Ser

485 490 495

Glu Leu Thr Asp Val Pro Glu His Ala Glu Leu Ile Ile Val Glu Asp

500 505 510

Pro His Gly Ser Met Ser Asp Arg Pro Ile Val Thr Arg Glu Gly Ile

515 520 525

Pro Val Leu Tyr Pro Tyr Arg Asp Gly Glu Val Ser Leu His Ile Asp

530 535 540

Gly Thr Gln His Ala Ala Asp Gly Arg Phe

545 550

<210>63

<211>2977

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2947)

<223>RXA02416

<400>63

agtcatgtgg ttcagactag cggaaacacc ttgttcgatg ctatgttcga aggtgtattt 60

tttgaagcga caaaaatcga ttttgaaggg cagttgagca ttg gct gat cgc ctc 115

Leu Ala Asp Arg Leu

1 5

gta gtg cgc gga gcg cgt gaa cat aac cta aaa ggc gtg gat att gat 163

Val Val Arg Gly Ala Arg Glu His Asn Leu Lys Gly Val Asp Ile Asp

10 15 20

ttg cca cgc gac tcg atg gtg gtg ttc acc ggc ctg tca ggt tcc ggt 211

Leu Pro Arg Asp Ser Met Val Val Phe Thr Gly Leu Ser Gly Ser Gly

25 30 35

aaa tca tca ctg gcc ttt gac acc atc ttt gcg gaa ggc cag cgc cgt 259

Lys Ser Ser Leu Ala Phe Asp Thr Ile Phe Ala Glu Gly Gln Arg Arg

40 45 50

tac gtg gag tcg ttg tcc agt tac gcc cgc atg ttc ttg ggg cag atg 307

Tyr Val Glu Ser Leu Ser Ser Tyr Ala Arg Met Phe Leu Gly Gln Met

55 60 65

gac aag ccg gac gtg gat ttg att gat gga tta tcc cea gcg gtc tcc 355

Asp Lys Pro Asp Val Asp Leu Ile Asp Gly Leu Ser Pro Ala Val Ser

70 75 80 85

att gac caa aaa tcc acc aac cgc aac cct cgg tcc aca gtc ggt acc 403

Ile Asp Gln Lys Ser Thr Asn Arg Asn Pro Arg Ser Thr Val Gly Thr

90 95 100

atc acg gaa gtc tat gac tac ctg cgt ctt ctg tac gcc cgc gct ggt 451

Ile Thr Glu Val Tyr Asp Tyr Leu Arg Leu Leu Tyr Ala Arg Ala Gly

105 110 115

acc gca cac tgc cca gtg tgt gat gcc cgc gtg gag cgt caa acc ccc 499

Thr Ala His Cys Pro Val Cys Asp Ala Arg Val Glu Arg Gln Thr Pro

120 125 130

cag cag atg gtg gac caa atc ctt ggc atg gag gag gga ctg aag ttc 547

Gln Gln Met Val Asp Gln Ile Leu Gly Met Glu Glu Gly Leu Lys Phe

135 140 145

caa atc ctt gcg cct gtg gtg cgt acc cgt aaa ggt gag ttc gtt gat 595

Gln Ile Leu Ala Pro Val Val Arg Thr Arg Lys Gly Glu Phe Val Asp

150 155 160 165

ctt ttc gca gat ctt gca tcc caa ggt tat tcc cgc gtg cgg gtt gat 643

Leu Phe Ala Asp Leu Ala Ser Gln Gly Tyr Ser Arg Val Arg Val Asp

170 175 180

ggg gaa gtg cac cag ctc tcg gat cct cca aag cta gaa aag cag atc 691

Gly Glu Val His Gln Leu Ser Asp Pro Pro Lys Leu Glu Lys Gln Ile

185 190 195

aag cac gat att gat gtt gtg gtt gac cgt ctg cag gta aaa gcc agc 739

Lys His Asp Ile Asp Val Val Val Asp Arg Leu Gln Val Lys Ala Ser

200 205 210

caa aag cag cgc ctg aca gac tct atg gaa acc gca ctt cgc ctg gcc 787

Gln Lys Gln Arg Leu Thr Asp Ser Met Glu Thr Ala Leu Arg Leu Ala

215 220 225

gat ggc gtg gct gtg ctg gag ttc gtt ggc ctg gag gaa gat gat ccg 835

Asp Gly Val Ala Val Leu Glu Phe Val Gly Leu Glu Glu Asp Asp Pro

230 235 240 245

aat agg ctt cgt cga ttc tct gaa aag atg agc tgc cct aac ggt cac 883

Asn Arg Leu Arg Arg Phe Ser Glu Lys Met Ser Cys Pro Asn Gly His

250 255 260

gcg ttg acg gtt gat gag ctg gag cct cgt gct ttt tcc ttc aac tct 931

Ala Leu Thr Val Asp Glu Leu Glu Pro Arg Ala Phe Ser Phe Asn Ser

265 270 275

cct tat ggc gcg tgt cct gcc tgt gat ggc ttg ggt gtg cgc acc gaa 979

Pro Tyr Gly Ala Cys Pro Ala Cys Asp Gly Leu Gly Val Arg Thr Glu

280 285 290

gtt gat att gat ctg atc atc cca gat cca gat gca cct gca act aaa 1027

Val Asp Ile Asp Leu Ile Ile Pro Asp Pro Asp Ala Pro Ala Thr Lys

295 300 305

gcg gtt cag ccc tgg aac tcc agc cca aac cac tct tac ttt gaa aag 1075

Ala Val Gln Pro Trp Asn Ser Ser Pro Asn His Ser Tyr Phe Glu Lys

310 315 320 325

ctc att gaa ggc ctg gcg aaa gcc ctc gga ttt gat ccg gaa act ccg 1123

Leu Ile Glu Gly Leu Ala Lys Ala Leu Gly Phe Asp Pro Glu Thr Pro

330 335 340

tac agt gag ctc acc gca gct caa aag aag gct ctg gtc tat gga tcg 1171

Tyr Ser Glu Leu Thr Ala Ala Gln Lys Lys Ala Leu Val Tyr Gly Ser

345 350 355

aag gaa gaa gta agc gtt cga tac aag aac cgc tac gga cgc gtg cgt 1219

Lys Glu Glu Val Ser Val Arg Tyr Lys Asn Arg Tyr Gly Arg Val Arg

360 365 370

tct tgg act gcg cct ttt gaa ggt gtc atg ggc tac ttt gat cgc aag 1267

Ser Trp Thr Ala Pro Phe Glu Gly Val Met Gly Tyr Phe Asp Arg Lys

375 380 385

ttg gag cag act gat tcc gaa acc caa aaa gac cga ctg ttg ggc tac 1315

Leu Glu Gln Thr Asp Ser Glu Thr Gln Lys Asp Arg Leu Leu Gly Tyr

390 395 400 405

acc cgt gaa gtg ccc tgc cca acc tgt aaa ggc gca cgc ctc aag ccg 1363

Thr Arg Glu Val Pro Cys Pro Thr Cys Lys Gly Ala Arg Leu Lys Pro

410 415 420

gaa atc ttg gcc gtt cgc cta gac tcc gga agc cat gga gcg ttg tcc 1411

Glu Ile Leu Ala Val Arg Leu Asp Ser Gly Ser His Gly Ala Leu Ser

425 430 435

att gct gga cta acc gcg ctg tcg gtg cat gaa gca ttc gag ttt ttg 1459

Ile Ala Gly Leu Thr Ala Leu Ser Val His Glu Ala Phe Glu Phe Leu

440 445 450

gat aac ctc aca ctg ggc aag cgc gag gaa atg atc gcg gga gct gtg 1507

Asp Asn Leu Thr Leu Gly Lys Arg Glu Glu Met Ile Ala Gly Ala Val

455 460 465

ctg aag gaa att cac gcc cgc ctg aaa ttc ctg ctt gac gtg ggc ctt 1555

Leu Lys Glu Ile His Ala Arg Leu Lys Phe Leu Leu Asp Val Gly Leu

470 475 480 485

tcc tac ctc acc ctt gat cgc gcc gca ggc acc ctg tct ggt ggt gaa 1603

Ser Tyr Leu Thr Leu Asp Arg Ala Ala Gly Thr Leu Ser Gly Gly Glu

490 495 500

gcg cag cgt atc cgc ctg gct act caa att ggt tcc ggt ctg gct ggt 1651

Ala Gln Arg Ile Arg Leu Ala Thr Gln Ile Gly Ser Gly Leu Ala Gly

505 510 515

gtg ctc tac gtc ttg gat gag cca tcc att ggt ctg cac caa cgt gac 1699

Val Leu Tyr Val Leu Asp Glu Pro Ser Ile Gly Leu His Gln Arg Asp

520 525 530

aac cag cgc ttg atc act acc ctt gag cat ctc cga gat atc gga aac 1747

Asn Gln Arg Leu Ile Thr Thr Leu Glu His Leu Arg Asp Ile Gly Asn

535 540 545

acg ctc att gtt gtg gaa cac gat gaa gac acc atc agg cgc gca gat 1795

Thr Leu Ile Val Val Glu His Asp Glu Asp Thr Ile Arg Arg Ala Asp

550 555 560 565

tgg ctc gtg gat att ggt cct cga gct ggt gaa ttt ggt ggc gaa gtg 1843

Trp Leu Val Asp Ile Gly Pro Arg Ala Gly Glu Phe Gly Gly Glu Val

570 575 580

gtc tac caa ggt gag ccg aag ggc att ttg gac tgc gaa gaa tcc ctc 1891

Val Tyr Gln Gly Glu Pro Lys Gly Ile Leu Asp Cys Glu Glu Ser Leu

585 590 595

aca ggt gct tac ttg tct ggt cgt cga acc ctg ggt gtt cct gat act 1939

Thr Gly Ala Tyr Leu Ser Gly Arg Arg Thr Leu Gly Val Pro Asp Thr

600 605 610

cgc cgt gag atc gac aaa gag cga cag ctc aag gtg gtt ggt gct agg 1987

Arg Arg Glu Ile Asp Lys Glu Arg Gln Leu Lys Val Val Gly Ala Arg

615 620 625

gaa aat aac ctg cag ggc atc gat gtg aaa atc cca ctg ggt gtg ctg 2035

Glu Asn Asn Leu Gln Gly Ile Asp Val Lys Ile Pro Leu Gly Val Leu

630 635 640 645

tgc tgc atc act ggt gtg tcg gga tct ggt aaa tcc acg ctg gtc aat 2083

Cys Cys Ile Thr Gly Val Ser Gly Ser Gly Lys Ser Thr Leu Val Asn

650 655 660

cag att ttg gcc aag gtt ctg gcc aac aaa ctc aac cgc gca cgc caa 2131

Gln Ile Leu Ala Lys Val Leu Ala Asn Lys Leu Asn Arg Ala Arg Gln

665 670 675

gtg cct ggt cgc gca aag cgg gtg gaa ggc ctc gag cac ttg gat aag 2179

Val Pro Gly Arg Ala Lys Arg Val Glu Gly Leu Glu His Leu Asp Lys

680 685 690

ttg gtc caa gtg gat cag tcg cca att ggt cgt act cca cgt tca aac 2227

Leu Val Gln Val Asp Gln Ser Pro Ile Gly Arg Thr Pro Arg Ser Asn

695 700 705

cca gcg acg tac acg ggt gtg ttt gat aaa gtc cgt aac ctt ttt gcc 2275

Pro Ala Thr Tyr Thr Gly Val Phe Asp Lys Val Arg Asn Leu Phe Ala

710 715 720 725

gag acc act gaa gcg aag gtc cgc ggt tac aag cct ggc cgc ttc tcc 2323

Glu Thr Thr Glu Ala Lys Val Arg Gly Tyr Lys Pro Gly Arg Phe Ser

730 735 740

ttc aat att aag ggt gga cgc tgc gaa gca tgt cag ggc gat ggc acg 2371

Phe Asn Ile Lys Gly Gly Arg Cys Glu Ala Cys Gln Gly Asp Gly Thr

745 750 755

ctg aag atc gaa atg aac ttc ctg ccc gac gtg tat gtt ccg tgt gaa 2419

Leu Lys Ile Glu Met Asn Phe Leu Pro Asp Val Tyr Val Pro Cys Glu

760 765 770

gtc tgt gat ggt cag cgc tac aac cgc gag acc ctc gag gtg aag tac 2467

Val Cys Asp Gly Gln Arg Tyr Asn Arg Glu Thr Leu Glu Val Lys Tyr

775 780 785

aag ggc aaa aac atc gct gaa gta ttg ggc atg ccg atc tct gag gct 2515

Lys Gly Lys Asn Ile Ala Glu Val Leu Gly Met Pro Ile Ser Glu Ala

790 795 800 805

gcg gac ttc ttt gag ccc atc acc tca att cac cga tac cta gca acg 2563

Ala Asp Phe Phe Glu Pro Ile Thr Ser Ile His Arg Tyr Leu Ala Thr

810 815 820

ctg gtt gat gtc ggc ctt ggc tat gtc cgt ttg ggc cag gca gca aca 2611

Leu Val Asp Val Gly Leu Gly Tyr Val Arg Leu Gly Gln Ala Ala Thr

825 830 835

acc ttg tct ggt ggt gaa gcc cag cgt gtg aaa ctt gcc gct gag ctg 2659

Thr Leu Ser Gly Gly Glu Ala Gln Arg Val Lys Leu Ala Ala Glu Leu

840 845 850

cag aag cgt tcc aac ggt cgc acc gtt tac atc ctc gat gag cca act 2707

Gln Lys Arg Ser Asn Gly Arg Thr Val Tyr Ile Leu Asp Glu Pro Thr

855 860 865

act ggt ttg cac ttt gaa gat att cgc aaa ctc atg atg gtg atc gaa 2755

Thr Gly Leu His Phe Glu Asp Ile Arg Lys Leu Met Met Val Ile Glu

870 875 880 885

ggc ctg gtg gac aag ggt aac tcc gtg atc atc atc gag cac aac ctc 2803

Gly Leu Val Asp Lys Gly Asn Ser Val Ile Ile Ile Glu His Asn Leu

890 895 900

gac gtg atc aag gct gcc gac tgg atc gtg gac atg ggt cca gag ggc 2851

Asp Val Ile Lys Ala Ala Asp Trp Ile Val Asp Met Gly Pro Glu Gly

905 910 915

gga agc ggc ggt gga acc gtg gtc gct gaa gga acc cca gag caa gtt 2899

Gly Ser Gly Gly Gly Thr Val Val Ala Glu Gly Thr Pro Glu Gln Val

920 925 930

gct gaa gtt gcg ggt tcc tac acc ggc caa ttc ctt aaa gag ttg ttg 2947

Ala Glu Val Ala Gly Ser Tyr Thr Gly Gln Phe Leu Lys Glu Leu Leu

935 940 945

taggagaaga tgaggggctt tcatgggaag 2977

<210>64

<211>949

<212>PRT

<213>谷氨酸棒状杆菌

<400>64

Leu Ala Asp Arg Leu Val Val Arg Gly Ala Arg Glu His Asn Leu Lys

1 5 10 15

Gly Val Asp Ile Asp Leu Pro Arg Asp Ser Met Val Val Phe Thr Gly

20 25 30

Leu Ser Gly Ser Gly Lys Ser Ser Leu Ala Phe Asp Thr Ile Phe Ala

35 40 45

Glu Gly Gln Arg Arg Tyr Val Glu Ser Leu Ser Ser Tyr Ala Arg Met

50 55 60

Phe Leu Gly Gln Met Asp Lys Pro Asp Val Asp Leu Ile Asp Gly Leu

65 70 75 80

Ser Pro Ala Val Ser Ile Asp Gln Lys Ser Thr Asn Arg Asn Pro Arg

85 90 95

Ser Thr Val Gly Thr Ile Thr Glu Val Tyr Asp Tyr Leu Arg Leu Leu

100 105 110

Tyr Ala Arg Ala Gly Thr Ala His Cys Pro Val Cys Asp Ala Arg Val

115 120 125

Glu Arg Gln Thr Pro Gln Gln Met Val Asp Gln Ile Leu Gly Met Glu

130 135 140

Glu Gly Leu Lys Phe Gln Ile Leu Ala Pro Val Val Arg Thr Arg Lys

145 150 155 160

Gly Glu Phe Val Asp Leu Phe Ala Asp Leu Ala Ser Gln Gly Tyr Ser

165 170 175

Arg Val Arg Val Asp Gly Glu Val His Gln Leu Ser Asp Pro Pro Lys

180 185 190

Leu Glu Lys Gln Ile Lys His Asp Ile Asp Val Val Val Asp Arg Leu

195 200 205

Gln Val Lys Ala Ser Gln Lys Gln Arg Leu Thr Asp Ser Met Glu Thr

210 215 220

Ala Leu Arg Leu Ala Asp Gly Val Ala Val Leu Glu Phe Val Gly Leu

225 230 235 240

Glu Glu Asp Asp Pro Asn Arg Leu Arg Arg Phe Ser Glu Lys Met Ser

245 250 255

Cys Pro Asn Gly His Ala Leu Thr Val Asp Glu Leu Glu Pro Arg Ala

260 265 270

Phe Ser Phe Asn Ser Pro Tyr Gly Ala Cys Pro Ala Cys Asp Gly Leu

275 280 285

Gly Val Arg Thr Glu Val Asp Ile Asp Leu Ile Ile Pro Asp Pro Asp

290 295 300

Ala Pro Ala Thr Lys Ala Val Gln Pro Trp Asn Ser Ser Pro Asn His

305 310 315 320

Ser Tyr Phe Glu Lys Leu Ile Glu Gly Leu Ala Lys Ala Leu Gly Phe

325 330 335

Asp Pro Glu Thr Pro Tyr Ser Glu Leu Thr Ala Ala Gln Lys Lys Ala

340 345 350

Leu Val Tyr Gly Ser Lys Glu Glu Val Ser Val Arg Tyr Lys Asn Arg

355 360 365

Tyr Gly Arg Val Arg Ser Trp Thr Ala Pro Phe Glu Gly Val Met Gly

370 375 380

Tyr Phe Asp Arg Lys Leu Glu Gln Thr Asp Ser Glu Thr Gln Lys Asp

385 390 395 400

Arg Leu Leu Gly Tyr Thr Arg Glu Val Pro Cys Pro Thr Cys Lys Gly

405 410 415

Ala Arg Leu Lys Pro Glu Ile Leu Ala Val Arg Leu Asp Ser Gly Ser

420 425 430

His Gly Ala Leu Ser Ile Ala Gly Leu Thr Ala Leu Ser Val His Glu

435 440 445

Ala Phe Glu Phe Leu Asp Asn Leu Thr Leu Gly Lys Arg Glu Glu Met

450 455 460

Ile Ala Gly Ala Val Leu Lys Glu Ile His Ala Arg Leu Lys Phe Leu

465 470 475 480

Leu Asp Val Gly Leu Ser Tyr Leu Thr Leu Asp Arg Ala Ala Gly Thr

485 490 495

Leu Ser Gly Gly Glu Ala Gln Arg Ile Arg Leu Ala Thr Gln Ile Gly

500 505 510

Ser Gly Leu Ala Gly Val Leu Tyr Val Leu Asp Glu Pro Ser Ile Gly

515 520 525

Leu His Gln Arg Asp Asn Gln Arg Leu Ile Thr Thr Leu Glu His Leu

530 535 540

Arg Asp Ile Gly Asn Thr Leu Ile Val Val Glu His Asp Glu Asp Thr

545 550 555 560

Ile Arg Arg Ala Asp Trp Leu Val Asp Ile Gly Pro Arg Ala Gly Glu

565 570 575

Phe Gly Gly Glu Val Val Tyr Gln Gly Glu Pro Lys Gly Ile Leu Asp

580 585 590

Cys Glu Glu Ser Leu Thr Gly Ala Tyr Leu Ser Gly Arg Arg Thr Leu

595 600 605

Gly Val Pro Asp Thr Arg Arg Glu Ile Asp Lys Glu Arg Gln Leu Lys

610 615 620

Val Val Gly Ala Arg Glu Asn Asn Leu Gln Gly Ile Asp Val Lys Ile

625 630 635 640

Pro Leu Gly Val Leu Cys Cys Ile Thr Gly Val Ser Gly Ser Gly Lys

645 650 655

Ser Thr Leu Val Asn Gln Ile Leu Ala Lys Val Leu Ala Asn Lys Leu

660 665 670

Asn Arg Ala Arg Gln Val Pro Gly Arg Ala Lys Arg Val Glu Gly Leu

675 680 685

Glu His Leu Asp Lys Leu Val Gln Val Asp Gln Ser Pro Ile Gly Arg

690 695 700

Thr Pro Arg Ser Asn Pro Ala Thr Tyr Thr Gly Val Phe Asp Lys Val

705 710 715 720

Arg Asn Leu Phe Ala Glu Thr Thr Glu Ala Lys Val Arg Gly Tyr Lys

725 730 735

Pro Gly Arg Phe Ser Phe Asn Ile Lys Gly Gly Arg Cys Glu Ala Cys

740 745 750

Gln Gly Asp Gly Thr Leu Lys Ile Glu Met Asn Phe Leu Pro Asp Val

755 760 765

Tyr Val Pro Cys Glu Val Cys Asp Gly Gln Arg Tyr Asn Arg Glu Thr

770 775 780

Leu Glu Val Lys Tyr Lys Gly Lys Asn Ile Ala Glu Val Leu Gly Met

785 790 795 800

Pro Ile Ser Glu Ala Ala Asp Phe Phe Glu Pro Ile Thr Ser Ile His

805 810 815

Arg Tyr Leu Ala Thr Leu Val Asp Val Gly Leu Gly Tyr Val Arg Leu

820 825 830

Gly Gln Ala Ala Thr Thr Leu Ser Gly Gly Glu Ala Gln Arg Val Lys

835 840 845

Leu Ala Ala Glu Leu Gln Lys Arg Ser Asn Gly Arg Thr Val Tyr Ile

850 855 860

Leu Asp Glu Pro Thr Thr Gly Leu His Phe Glu Asp Ile Arg Lys Leu

865 870 875 880

Met Met Val Ile Glu Gly Leu Val Asp Lys Gly Asn Ser Val Ile Ile

885 890 895

Ile Glu His Asn Leu Asp Val Ile Lys Ala Ala Asp Trp Ile Val Asp

900 905 910

Met Gly Pro Glu Gly Gly Ser Gly Gly Gly Thr Val Val Ala Glu Gly

915 920 925

Thr Pro Glu Gln Val Ala Glu Val Ala Gly Ser Tyr Thr Gly Gln Phe

930 935 940

Leu Lys Glu Leu Leu

945

<210>65

<211>697

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(667)

<223>RXA02418

<400>65

gacacggact agtgcccgtg tataactctt gaaagtcaga tctcccgttc atcctagatg 60

atgaacgggt ttttggtctg cataggggca atcacctcaa gtg gtt cgg tac gtc 115

Val Val Arg Tyr Val

1 5

aaa ttt tcc cgc act gct aac aga gga gtc cac atc agc gct gaa gct 163

Lys Phe Ser Arg Thr Ala Asn Arg Gly Val His Ile Ser Ala Glu Ala

10 15 20

cgc att aat gag cgc atc cga gtt ccc gaa gtc cgc ctt gtc gga cct 211

Arg Ile Asn Glu Arg Ile Arg Val Pro Glu Val Arg Leu Val Gly Pro

25 30 35

aac ggt gag caa gta ggc atc gtc cgt atc gaa gat gcc cgc aag ctc 259

Asn Gly Glu Gln Val Gly Ile Val Arg Ile Glu Asp Ala Arg Lys Leu

40 45 50

gca ttc gac gca gac cta gac ctg gtc gag gtc gca ccc aac gcc aaa 307

Ala Phe Asp Ala Asp Leu Asp Leu Val Glu Val Ala Pro Asn Ala Lys

55 60 65

cct cca gtc tgc aag atc atg gac tac gga aag ttc aag tac gaa gcg 355

Pro Pro Val Cys Lys Ile Met Asp Tyr Gly Lys Phe Lys Tyr Glu Ala

70 75 80 85

gcc caa aag gct cgt gag tca cgc aag aat cag cag cag acc gtg gtc 403

Ala Gln Lys Ala Arg Glu Ser Arg Lys Asn Gln Gln Gln Thr Val Val

90 95 100

aaa gag caa aag ctt cgt ccc aag atc gat gat cat gat tat gag acg 451

Lys Glu Gln Lys Leu Arg Pro Lys Ile Asp Asp His Asp Tyr Glu Thr

105 110 115

aag aag aac aat gtg atc cga ttc ctt gaa aag gga tca aag gtc aaa 499

Lys Lys Asn Asn Val Ile Arg Phe Leu Glu Lys Gly Ser Lys Val Lys

120 125 130

gtc acg atc atg ttc cgt ggt cgt gag cag gct cgc cca gag ctt ggc 547

Val Thr Ile Met Phe Arg Gly Arg Glu Gln Ala Arg Pro Glu Leu Gly

135 140 145

tac agg ctc ctc gag cga ctg gca aac gat gtc gta gat ttt ggc atc 595

Tyr Arg Leu Leu Glu Arg Leu Ala Asn Asp Val Val Asp Phe Gly Ile

150 155 160 165

gtg gaa acc cgc gca aag cag gac gga cga aac atg aca atg gtt ctc 643

Val Glu Thr Arg Ala Lys Gln Asp Gly Arg Asn Met Thr Met Val Leu

170 175 180

ggt ccg gtg cgc aag ggc aag aaa taatcacgaa tagggtttaa ggacaacttt 697

Gly Pro Val Arg Lys Gly Lys Lys

185

<210>66

<211>189

<212>PRT

<213>谷氨酸棒状杆菌

<400>66

Val Val Arg Tyr Val Lys Phe Ser Arg Thr Ala Asn Arg Gly Val His

1 5 10 15

Ile Ser Ala Glu Ala Arg Ile Asn Glu Arg Ile Arg Val Pro Glu Val

20 25 30

Arg Leu Val Gly Pro Asn Gly Glu Gln Val Gly Ile Val Arg Ile Glu

35 40 45

Asp Ala Arg Lys Leu Ala Phe Asp Ala Asp Leu Asp Leu Val Glu Val

50 55 60

Ala Pro Asn Ala Lys Pro Pro Val Cys Lys Ile Met Asp Tyr Gly Lys

65 70 75 80

Phe Lys Tyr Glu Ala Ala Gln Lys Ala Arg Glu Ser Arg Lys Asn Gln

85 90 95

Gln Gln Thr Val Val Lys Glu Gln Lys Leu Arg Pro Lys Ile Asp Asp

100 105 110

His Asp Tyr Glu Thr Lys Lys Asn Asn Val Ile Arg Phe Leu Glu Lys

115 120 125

Gly Ser Lys Val Lys Val Thr Ile Met Phe Arg Gly Arg Glu Gln Ala

130 135 140

Arg Pro Glu Leu Gly Tyr Arg Leu Leu Glu Arg Leu Ala Asn Asp Val

145 150 155 160

Val Asp Phe Gly Ile Val Glu Thr Arg Ala Lys Gln Asp Gly Arg Asn

165 170 175

Met Thr Met Val Leu Gly Pro Val Arg Lys Gly Lys Lys

180 185

<210>67

<211>2419

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2389)

<223>RXA02429

<400>67

tcttacccac cagtgcaatg taggtcacgt cgtatcacgt ctgagggtga ttgagtaggg 60

ttaaacagat gaattcattt agctcaccgg aggtataacc gtg gcc ggt ttt gat 115

Val Ala Gly Phe Asp

1 5

tgg ttt tgg aag gcc ctt ggc ggc aaa tcg ggc aga aac caa aaa cgt 163

Trp Phe Trp Lys Ala Leu Gly Gly Lys Ser Gly Arg Asn Gln Lys Arg

10 15 20

agc gtg gca att gtc aat cag gta gaa aac cat gca gcg gaa tta gac 211

Ser Val Ala Ile Val Asn Gln Val Glu Asn His Ala Ala Glu Leu Asp

25 30 35

gcg ctg gat gat gtt gca ttg gcg cag cgt gcc aag gat cta gcc agt 259

Ala Leu Asp Asp Val Ala Leu Ala Gln Arg Ala Lys Asp Leu Ala Ser

40 45 50

ggt gga cgc att gac aat cat gcg gaa ttc ctc gcc att ttg ggt gtg 307

Gly Gly Arg Ile Asp Asn His Ala Glu Phe Leu Ala Ile Leu Gly Val

55 60 65

gca tcg cag cgg aca ttg ggg ctg aag ccg tat ccg gtg caa tca cag 355

Ala Ser Gln Arg Thr Leu Gly Leu Lys Pro Tyr Pro Val Gln Ser Gln

70 75 80 85

gcg gtg ttg cgt ctc att gaa ggc gat gtg gtg cac atg gct acc ggt 403

Ala Val Leu Arg Leu Ile Glu Gly Asp Val Val His Met Ala Thr Gly

90 95 100

gag ggc aag act ttg gtg ggc gcg atg gcg gcc acc ggt ctg ggg ttg 451

Glu Gly Lys Thr Leu Val Gly Ala Met Ala Ala Thr Gly Leu Gly Leu

105 110 115

atg ggc aag cga gtc cat tcg att acc gtc aat gat tat ttg gcg gtg 499

Met Gly Lys Arg Val His Ser Ile Thr Val Asn Asp Tyr Leu Ala Val

120 125 130

cgc gat gcc gaa tgg atg cgg cca ttg gtc gaa ttt ttc ggt ctg agc 547

Arg Asp Ala Glu Trp Met Arg Pro Leu Val Glu Phe Phe Gly Leu Ser

135 140 145

gtg gcg agc atc agc gag aag atg gat gca ggg gag cgt cga caa gca 595

Val Ala Ser Ile Ser Glu Lys Met Asp Ala Gly Glu Arg Arg Gln Ala

150 155 160 165

tat aaa gcc gca att gtc tac gga cct gtc aat gaa atc ggc ttt gac 643

Tyr Lys Ala Ala Ile Val Tyr Gly Pro Val Asn Glu Ile Gly Phe Asp

170 175 180

gtg ctg cgt gat cag cta att acc cgg cgc gaa gac gcc gtg cag cat 691

Val Leu Arg Asp Gln Leu Ile Thr Arg Arg Glu Asp Ala Val Gln His

185 190 195

ggc gcc gac gtc gcg att atc gat gag gcc gat tcc gtg ctt gtc gac 739

Gly Ala Asp Val Ala Ile Ile Asp Glu Ala Asp Ser Val Leu Val Asp

200 205 210

gag gcc ctg gtg cca ctc gtc ctc gcc ggc aac cag ccc ggc cat gcg 787

Glu Ala Leu Val Pro Leu Val Leu Ala Gly Asn Gln Pro Gly His Ala

215 220 225

ccg cgc ggc aaa atc acc gat gtg gtg cgc tcg ttg aaa gaa aac gac 835

Pro Arg Gly Lys Ile Thr Asp Val Val Arg Ser Leu Lys Glu Asn Asp

230 235 240 245

gat tac acc atc gac gat gat cgt cgc aac gtc ttc ctc acc gac aag 883

Asp Tyr Thr Ile Asp Asp Asp Arg Arg Asn Val Phe Leu Thr Asp Lys

250 255 260

ggt gcc gcc aaa tta gag cag cag ctg ggc atc agc agc ctc tac gac 931

Gly Ala Ala Lys Leu Glu Gln Gln Leu Gly Ile Ser Ser Leu Tyr Asp

265 270 275

gat gag cac gtc ggc tcg acg ctc gtg cag gtc aac ctc gcc ctc cac 979

Asp Glu His Val Gly Ser Thr Leu Val Gln Val Asn Leu Ala Leu His

280 285 290

gcg cag gca ctg ctc atc cgc gac atc cac tac atc gtc cgc gac agc 1027

Ala Gln Ala Leu Leu Ile Arg Asp Ile His Tyr Ile Val Arg Asp Ser

295 300 305

aag gtc ttg ctt atc gac gcc tcc cgc ggc cgt gtc gcc gac ctg cag 1075

Lys Val Leu Leu Ile Asp Ala Ser Arg Gly Arg Val Ala Asp Leu Gln

310 315 320 325

cgc tgg ccc gac ggc ctg caa gca gca gtg gag gcc aag gaa ggt ctc 1123

Arg Trp Pro Asp Gly Leu Gln Ala Ala Val Glu Ala Lys Glu Gly Leu

330 335 340

gcg gtt tct gaa ggc ggc aag atc ctt gac acc atc aca ctt cag gcg 1171

Ala Val Ser Glu Gly Gly Lys Ile Leu Asp Thr Ile Thr Leu Gln Ala

345 350 355

ttg att ggt cgc tac cca atg gca tgc ggc atg aca ggt acc gcc gtg 1219

Leu Ile Gly Arg Tyr Pro Met Ala Cys Gly Met Thr Gly Thr Ala Val

360 365 370

gag gca acc gat cag cta cgc acc ttc tat gac ttg cat gtt tct gtc 1267

Glu Ala Thr Asp Gln Leu Arg Thr Phe Tyr Asp Leu His Val Ser Val

375 380 385

att gag cgc aat cat ccg ctg aag cgc ttt gat gaa gct gac cgt atc 1315

Ile Glu Arg Asn His Pro Leu Lys Arg Phe Asp Glu Ala Asp Arg Ile

390 395 400 405

tac gcc acc atg gcg gag aaa aac cgc gcc atc atc gat gaa atc gca 1363

Tyr Ala Thr Met Ala Glu Lys Asn Arg Ala Ile Ile Asp Glu Ile Ala

410 415 420

ctc ctt cac agc acg ggg cag cca gtc ctg gtg ggt acc cac gat gtg 1411

Leu Leu His Ser Thr Gly Gln Pro Val Leu Val Gly Thr His Asp Val

425 430 435

gca gag tcg gaa gaa ctc gcc act gca ctg cgt gaa ctc aac atc gaa 1459

Ala Glu Ser Glu Glu Leu Ala Thr Ala Leu Arg Glu Leu Asn Ile Glu

440 445 450

gta agc gtt ctc aac gcc aag aat gat gcc gaa gaa gcc cag atc atc 1507

Val Ser Val Leu Asn Ala Lys Asn Asp Ala Glu Glu Ala Gln Ile Ile

455 460 465

gca gag gct ggc gat att gga cga gtg acc gtt tcc act cag atg gcc 1555

Ala Glu Ala Gly Asp Ile Gly Arg Val Thr Val Ser Thr Gln Met Ala

470 475 480 485

ggc cgc ggt acc gat att cgc ctc ggt ggc gcc gat gaa gcc gac tac 1603

Gly Arg Gly Thr Asp Ile Arg Leu Gly Gly Ala Asp Glu Ala Asp Tyr

490 495 500

gat gaa gtg gtg aaa ctc ggt gga ctc gcc gtt atc ggc acc gcc cgc 1651

Asp Glu Val Val Lys Leu Gly Gly Leu Ala Val Ile Gly Thr Ala Arg

505 510 515

cac cgt tct cag cgc ctg gac aac cag ctg cgc gga cgt gcg gga cga 1699

His Arg Ser Gln Arg Leu Asp Asn Gln Leu Arg Gly Arg Ala Gly Arg

520 525 530

caa gga gat cca ggc ctg agc ctt ttc ttt gtc tcc ctc gat gat gat 1747

Gln Gly Asp Pro Gly Leu Ser Leu Phe Phe Val Ser Leu Asp Asp Asp

535 540 545

gtg gtg gtc tca ggc ggg tca agg gag agc gtg agc gcg caa ccc gat 1795

Val Val Val Ser Gly Gly Ser Arg Glu Ser Val Ser Ala Gln Pro Asp

550 555 560 565

gcc acc ggg ctg att gac tca gat cgc atc cgc gat tgg gtc gga cac 1843

Ala Thr Gly Leu Ile Asp Ser Asp Arg Ile Arg Asp Trp Val Gly His

570 575 580

tgc cag cgc gtc acc gaa gga cag ctg ctg gaa atc cac tcc cag agc 1891

Cys Gln Arg Val Thr Glu Gly Gln Leu Leu Glu Ile His Ser Gln Ser

585 590 595

tgg aat tac aac aag ctc ctt gcc gat caa cgc gtg atc att gac gag 1939

Trp Asn Tyr Asn Lys Leu Leu Ala Asp Gln Arg Val Ile Ile Asp Glu

600 605 610

cgc cgc gaa cgc ctc ctc gac acc gcc tta gcg tgg gag gaa ctg gca 1987

Arg Arg Glu Arg Leu Leu Asp Thr Ala Leu Ala Trp Glu Glu Leu Ala

615 620 625

cag cat gca cca gcg cgg gct gca gag ctt gaa gac ctt gat cag tcc 2035

Gln His Ala Pro Ala Arg Ala Ala Glu Leu Glu Asp Leu Asp Gln Ser

630 635 640 645

gtg agg gaa cag gca gca cga gac atc atg ctg tac cac ctc gat tac 2083

Val Arg Glu Gln Ala Ala Arg Asp Ile Met Leu Tyr His Leu Asp Tyr

650 655 660

aac tgg tca gag cac ctc gcg ttg atg gat gat gtc cgc gaa tcc att 2131

Asn Trp Ser Glu His Leu Ala Leu Met Asp Asp Val Arg Glu Ser Ile

665 670 675

cac ctg cgc gcc atc gcc agg gaa acc ccc ctt gat gaa tac cac cgc 2179

His Leu Arg Ala Ile Ala Arg Glu Thr Pro Leu Asp Glu Tyr His Arg

680 685 690

atc gct gtg cgt gaa ttc aag gat ttg gca caa cgc gct gtc gat gat 2227

Ile Ala Val Arg Glu Phe Lys Asp Leu Ala Gln Arg Ala Val Asp Asp

695 700 705

gcg gtg tcc acg ttc aag tct gtg acc atc gat cac gag ggt gcc cat 2275

Ala Val Ser Thr Phe Lys Ser Val Thr Ile Asp His Glu Gly Ala His

710 715 720 725

ttg gat gat gag ggc ttg gcg cgt cca tca gca acg tgg acc tac atg 2323

Leu Asp Asp Glu Gly Leu Ala Arg Pro Ser Ala Thr Trp Thr Tyr Met

730 735 740

gtc tct gac aac cca ctt gcg ggt agt ggt aac tca gtg atc agt ggc 2371

Val Ser Asp Asn Pro Leu Ala Gly Ser Gly Asn Ser Val Ile Ser Gly

745 750 755

ata gga aat atc ttt aga taacctgaga actatgaaat tccagctcac 2419

Ile Gly Asn Ile Phe Arg

760

<210>68

<211>763

<212>PRT

<213>谷氨酸棒状杆菌

<400>68

Val Ala Gly Phe Asp Trp Phe Trp Lys Ala Leu Gly Gly Lys Ser Gly

1 5 10 15

Arg Asn Gln Lys Arg Ser Val Ala Ile Val Asn Gln Val Glu Asn His

20 25 30

Ala Ala Glu Leu Asp Ala Leu Asp Asp Val Ala Leu Ala Gln Arg Ala

35 40 45

Lys Asp Leu Ala Ser Gly Gly Arg Ile Asp Asn His Ala Glu Phe Leu

50 55 60

Ala Ile Leu Gly Val Ala Ser Gln Arg Thr Leu Gly Leu Lys Pro Tyr

65 70 75 80

Pro Val Gln Ser Gln Ala Val Leu Arg Leu Ile Glu Gly Asp Val Val

85 90 95

His Met Ala Thr Gly Glu Gly Lys Thr Leu Val Gly Ala Met Ala Ala

100 105 110

Thr Gly Leu Gly Leu Met Gly Lys Arg Val His Ser Ile Thr Val Asn

115 120 125

Asp Tyr Leu Ala Val Arg Asp Ala Glu Trp Met Arg Pro Leu Val Glu

130 135 140

Phe Phe Gly Leu Ser Val Ala Ser Ile Ser Glu Lys Met Asp Ala Gly

145 150 155 160

Glu Arg Arg Gln Ala Tyr Lys Ala Ala Ile Val Tyr Gly Pro Val Asn

165 170 175

Glu Ile Gly Phe Asp Val Leu Arg Asp Gln Leu Ile Thr Arg Arg Glu

180 185 190

Asp Ala Val Gln His Gly Ala Asp Val Ala Ile Ile Asp Glu Ala Asp

195 200 205

Ser Val Leu Val Asp Glu Ala Leu Val Pro Leu Val Leu Ala Gly Asn

210 215 220

Gln Pro Gly His Ala Pro Arg Gly Lys Ile Thr Asp Val Val Arg Ser

225 230 235 240

Leu Lys Glu Asn Asp Asp Tyr Thr Ile Asp Asp Asp Arg Arg Asn Val

245 250 255

Phe Leu Thr Asp Lys Gly Ala Ala Lys Leu Glu Gln Gln Leu Gly Ile

260 265 270

Ser Ser Leu Tyr Asp Asp Glu His Val Gly Ser Thr Leu Val Gln Val

275 280 285

Asn Leu Ala Leu His Ala Gln Ala Leu Leu Ile Arg Asp Ile His Tyr

290 295 300

Ile Val Arg Asp Ser Lys Val Leu Leu Ile Asp Ala Ser Arg Gly Arg

305 310 315 320

Val Ala Asp Leu Gln Arg Trp Pro Asp Gly Leu Gln Ala Ala Val Glu

325 330 335

Ala Lys Glu Gly Leu Ala Val Ser Glu Gly Gly Lys Ile Leu Asp Thr

340 345 350

Ile Thr Leu Gln Ala Leu Ile Gly Arg Tyr Pro Met Ala Cys Gly Met

355 360 365

Thr Gly Thr Ala Val Glu Ala Thr Asp Gln Leu Arg Thr Phe Tyr Asp

370 375 380

Leu His Val Ser Val Ile Glu Arg Asn His Pro Leu Lys Arg Phe Asp

385 390 395 400

Glu Ala Asp Arg Ile Tyr Ala Thr Met Ala Glu Lys Asn Arg Ala Ile

405 410 415

Ile Asp Glu Ile Ala Leu Leu His Ser Thr Gly Gln Pro Val Leu Val

420 425 430

Gly Thr His Asp Val Ala Glu Ser Glu Glu Leu Ala Thr Ala Leu Arg

435 440 445

Glu Leu Asn Ile Glu Val Ser Val Leu Asn Ala Lys Asn Asp Ala Glu

450 455 460

Glu Ala Gln Ile Ile Ala Glu Ala Gly Asp Ile Gly Arg Val Thr Val

465 470 475 480

Ser Thr Gln Met Ala Gly Arg Gly Thr Asp Ile Arg Leu Gly Gly Ala

485 490 495

Asp Glu Ala Asp Tyr Asp Glu Val Val Lys Leu Gly Gly Leu Ala Val

500 505 510

Ile Gly Thr Ala Arg His Arg Ser Gln Arg Leu Asp Asn Gln Leu Arg

515 520 525

Gly Arg Ala Gly Arg Gln Gly Asp Pro Gly Leu Ser Leu Phe Phe Val

530 535 540

Ser Leu Asp Asp Asp Val Val Val Ser Gly Gly Ser Arg Glu Ser Val

545 550 555 560

Ser Ala Gln Pro Asp Ala Thr Gly Leu Ile Asp Ser Asp Arg Ile Arg

565 570 575

Asp Trp Val Gly His Cys Gln Arg Val Thr Glu Gly Gln Leu Leu Glu

580 585 590

Ile His Ser Gln Ser Trp Asn Tyr Asn Lys Leu Leu Ala Asp Gln Arg

595 600 605

Val Ile Ile Asp Glu Arg Arg Glu Arg Leu Leu Asp Thr Ala Leu Ala

610 615 620

Trp Glu Glu Leu Ala Gln His Ala Pro Ala Arg Ala Ala Glu Leu Glu

625 630 635 640

Asp Leu Asp Gln Ser Val Arg Glu Gln Ala Ala Arg Asp Ile Met Leu

645 650 655

Tyr His Leu Asp Tyr Asn Trp Ser Glu His Leu Ala Leu Met Asp Asp

660 665 670

Val Arg Glu Ser Ile His Leu Arg Ala Ile Ala Arg Glu Thr Pro Leu

675 680 685

Asp Glu Tyr His Arg Ile Ala Val Arg Glu Phe Lys Asp Leu Ala Gln

690 695 700

Arg Ala Val Asp Asp Ala Val Ser Thr Phe Lys Ser Val Thr Ile Asp

705 710 715 720

His Glu Gly Ala His Leu Asp Asp Glu Gly Leu Ala Arg Pro Ser Ala

725 730 735

Thr Trp Thr Tyr Met Val Ser Asp Asn Pro Leu Ala Gly Ser Gly Asn

740 745 750

Ser Val Ile Ser Gly Ile Gly Asn Ile Phe Arg

755 760

<210>69

<211>1582

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1552)

<223>RXA02431

<400>69

ggtggctgag cttgtcggca ttgtgctggt tgtcatcgca gttgctttgc gacgcccctc 60

ctagcggttt cccacaccgc cagtcttctc aaactaatcg ttg acc tgt tcg att 115

Leu Thr Cys Ser Ile

1 5

aac cta att ttc ggc tgg tca act acc ata aaa agc atg caa cgc tgg 163

Asn Leu Ile Phe Gly Trp Ser Thr Thr Ile Lys Ser Met Gln Arg Trp

10 15 20

gtg ctt cac atc gat atg gat gcc ttc ttc gca tcc tgc gaa caa ctg 211

Val Leu His Ile Asp Met Asp Ala Phe Phe Ala Ser Cys Glu Gln Leu

25 30 35

acc cgg ccc act tta aga ggc cgc ccc gtc ttg gtc ggt gga gtc tcc 259

Thr Arg Pro Thr Leu Arg Gly Arg Pro Val Lau Val Gly Gly Val Ser

40 45 50

ggt agg gga gtt gtc gcc gga gca tcc tat gaa gcc aga aaa ttt ggc 307

Gly Arg Gly Val Val Ala Gly Ala Ser Tyr Glu Ala Arg Lys Phe Gly

55 60 65

gcc cgc tca gcg atg ccc atg cac caa gcc aaa gcc cga gta ggt ttt 355

Ala Arg Ser Ala Met Pro Met His Gln Ala Lys Ala Arg Val Gly Phe

70 75 80 85

ggg gca gtg gtg gtg aca ccc cgt cat atc gtt tac tcc gca gcc tcg 403

Gly Ala Val Val Val Thr Pro Arg His Ile Val Tyr Ser Ala Ala Ser

90 95 100

cgc cgg gtg ttc caa atc gtg gaa aaa cgc gcc gga att gtc gaa cgc 451

Arg Arg Val Phe Gln Ile Val Glu Lys Arg Ala Gly Ile Val Glu Arg

105 110 115

ctc agc atc gat gaa ggc ttc atg gaa cca gag gct ctc gtt gga gcc 499

Leu Ser Ile Asp Glu Gly Phe Met Glu Pro Glu Ala Leu Val Gly Ala

120 125 130

acc cca gaa gag gtg aaa cag tgg gcg gaa gaa tta cgc gcg gaa att 547

Thr Pro Glu Glu Val Lys Gln Trp Ala Glu Glu Leu Arg Ala Glu Ile

135 140 145

aaa gaa gtt act ggc tta ccc tcc tcg gtt ggt gct ggc tcc ggt aag 595

Lys Glu Val Thr Gly Leu Pro Ser Ser Val Gly Ala Gly Ser Gly Lys

150 155 160 165

cag atc gcc aaa att ggt tca ggc gaa gca aag cca gat ggt gtg ttt 643

Gln Ile Ala Lys Ile Gly Ser Gly Glu Ala Lys Pro Asp Gly Val Phe

170 175 180

gtc gtg cca gta gac aag caa cat gac ttg ctt gat cca ctt cct gtg 691

Val Val Pro Val Asp Lys Gln His Asp Leu Leu Asp Pro Leu Pro Val

185 190 195

ggc gca ctt tgg gga gtg ggt cct gtg aca ggc tcc aag ctt gcc tca 739

Gly Ala Leu Trp Gly Val Gly Pro Val Thr Gly Ser Lys Leu Ala Ser

200 205 210

atg ggg gtg gaa aca att ggt gat cta gca gcg cta acc caa aaa gaa 787

Met Gly Val Glu Thr Ile Gly Asp Leu Ala Ala Leu Thr Gln Lys Glu

215 220 225

gta gaa atc agc ctc ggt gca acc atc gga ata tca ctg tgg aac ctt 835

Val Glu Ile Ser Leu Gly Ala Thr Ile Gly Ile Ser Leu Trp Asn Leu

230 235 240 245

gcc cga gga atc gac gac cgc cct gtg gaa ccc cgc gcc gaa gca aaa 883

Ala Arg Gly Ile Asp Asp Arg Pro Val Glu Pro Arg Ala Glu Ala Lys

250 255 260

cag atc tcc caa gag cac acc tat gaa aaa gac ctc ctc acc agg caa 931

Gln Ile Ser Gln Glu His Thr Tyr Glu Lys Asp Leu Leu Thr Arg Gln

265 270 275

caa gta gat gct gcc atc att cga tca gcc gaa ggc gca cac cga cgg 979

Gln Val Asp Ala Ala Ile Ile Arg Ser Ala Glu Gly Ala His Arg Arg

280 285 290

ctc ctc aaa gac gga cgc ggt gcc aga act gtc agc gtg aaa ctg cgg 1027

Leu Leu Lys Asp Gly Arg Gly Ala Arg Thr Val Ser Val Lys Leu Arg

295 300 305

atg gcc gac ttt cgt att gag tct cgt tcc tac acc ttg tcc tat gcc 1075

Met Ala Asp Phe Arg Ile Glu Ser Arg Ser Tyr Thr Leu Ser Tyr Ala

310 315 320 325

acc gat gat tac gca act ctt gag gca aca gca ttc cga ctt gcc cgc 1123

Thr Asp Asp Tyr Ala Thr Leu Glu Ala Thr Ala Phe Arg Leu Ala Arg

330 335 340

tac ccc gga gaa gta ggc ccc atc cgc ctt gtc gga gta agt ttt tct 1171

Tyr Pro Gly Glu Val Gly Pro Ile Arg Leu Val Gly Val Ser Phe Ser

345 350 355

ggt ttg gaa gaa tcc cgc caa gac atc ctc ttc ccg gaa ctt gac caa 1219

Gly Leu Glu Glu Ser Arg Gln Asp Ile Leu Phe Pro Glu Leu Asp Gln

360 365 370

caa atc atc gta cca cca gca ccc gac acc gat tat gag gta ggc gtg 1267

Gln Ile Ile Val Pro Pro Ala Pro Asp Thr Asp Tyr Glu Val Gly Val

375 380 385

caa tcc tct tct agt tcc gaa agt act caa gtt gaa gcg ccg caa gat 1315

Gln Ser Ser Ser Ser Ser Glu Ser Thr Gln Val Glu Ala Pro Gln Asp

390 395 400 405

gtc gcg ttg agt atg tgg tgc gca acg caa gat gtc tac cac cca gaa 1363

Val Ala Leu Ser Met Trp Cys Ala Thr Gln Asp Val Tyr His Pro Glu

410 415 420

tat ggc cac ggt tgg gta caa ggt gcc ggt cac ggt gtt gta tca gta 1411

Tyr Gly His Gly Trp Val Gln Gly Ala Gly His Gly Val Val Ser Val

425 430 435

cgt ttt gaa acc cgc agc acc aca aaa ggg cga act aaa agt ttt tcc 1459

Arg Phe Glu Thr Arg Ser Thr Thr Lys Gly Arg Thr Lys Ser Phe Ser

440 445 450

atg gat gac ccg gac ctc acc ccg gca gac cct cta gat agt ttg gat 1507

Met Asp Asp Pro Asp Leu Thr Pro Ala Asp Pro Leu Asp Ser Leu Asp

455 460 465

tgg gct gac tgg ttt gct gaa aat ggt gaa acg ggg gat gac gaa 1552

Trp Ala Asp Trp Phe Ala Glu Asn Gly Glu Thr Gly Asp Asp Glu

470 475 480

tagggtttca tcgggtttcg gggtgctttt 1582

<210>70

<211>484

<212>PRT

<213>谷氨酸棒状杆菌

<400>70

Leu Thr Cys Ser Ile Asn Leu Ile Phe Gly Trp Ser Thr Thr Ile Lys

1 5 10 15

Ser Met Gln Arg Trp Val Leu His Ile Asp Met Asp Ala Phe Phe Ala

20 25 30

Ser Cys Glu Gln Leu Thr Arg Pro Thr Leu Arg Gly Arg Pro Val Leu

35 40 45

Val Gly Gly Val Ser Gly Arg Gly Val Val Ala Gly Ala Ser Tyr Glu

50 55 60

Ala Arg Lys Phe Gly Ala Arg Ser Ala Met Pro Met His Gln Ala Lys

65 70 75 80

Ala Arg Val Gly Phe Gly Ala Val Val Val Thr Pro Arg His Ile Val

85 90 95

Tyr Ser Ala Ala Ser Arg Arg Val Phe Gln Ile Val Glu Lys Arg Ala

100 105 110

Gly Ile Val Glu Arg Leu Ser Ile Asp Glu Gly Phe Met Glu Pro Glu

115 120 125

Ala Leu Val Gly Ala Thr Pro Glu Glu Val Lys Gln Trp Ala Glu Glu

130 135 140

Leu Arg Ala Glu Ile Lys Glu Val Thr Gly Leu Pro Ser Ser Val Gly

145 150 155 160

Ala Gly Ser Gly Lys Gln Ile Ala Lys Ile Gly Ser Gly Glu Ala Lys

165 170 175

Pro Asp Gly Val Phe Val Val Pro Val Asp Lys Gln His Asp Leu Leu

180 185 190

Asp Pro Leu Pro Val Gly Ala Leu Trp Gly Val Gly Pro Val Thr Gly

195 200 205

Ser Lys Leu Ala Ser Met Gly Val Glu Thr Ile Gly Asp Leu Ala Ala

210 215 220

Leu Thr Gln Lys Glu Val Glu Ile Ser Leu Gly Ala Thr Ile Gly Ile

225 230 235 240

Ser Leu Trp Asn Leu Ala Arg Gly Ile Asp Asp Arg Pro Val Glu Pro

245 250 255

Arg Ala Glu Ala Lys Gln Ile Ser Gln Glu His Thr Tyr Glu Lys Asp

260 265 270

Leu Leu Thr Arg Gln Gln Val Asp Ala Ala Ile Ile Arg Ser Ala Glu

275 280 285

Gly Ala His Arg Arg Leu Leu Lys Asp Gly Arg Gly Ala Arg Thr Val

290 295 300

Ser Val Lys Leu Arg Met Ala Asp Phe Arg Ile Glu Ser Arg Ser Tyr

305 310 315 320

Thr Leu Ser Tyr Ala Thr Asp Asp Tyr Ala Thr Leu Glu Ala Thr Ala

325 330 335

Phe Arg Leu Ala Arg Tyr Pro Gly Glu Val Gly Pro Ile Arg Leu Val

340 345 350

Gly Val Ser Phe Ser Gly Leu Glu Glu Ser Arg Gln Asp Ile Leu Phe

355 360 365

Pro Glu Leu Asp Gln Gln Ile Ile Val Pro Pro Ala Pro Asp Thr Asp

370 375 380

Tyr Glu Val Gly Val Gln Ser Ser Ser Ser Ser Glu Ser Thr Gln Val

385 390 395 400

Glu Ala Pro Gln Asp Val Ala Leu Ser Met Trp Cys Ala Thr Gln Asp

405 410 415

Val Tyr His Pro Glu Tyr Gly His Gly Trp Val Gln Gly Ala Gly His

420 425 430

Gly Val Val Ser Val Arg Phe Glu Thr Arg Ser Thr Thr Lys Gly Arg

435 440 445

Thr Lys Ser Phe Ser Met Asp Asp Pro Asp Leu Thr Pro Ala Asp Pro

450 455 460

Leu Asp Ser Leu Asp Trp Ala Asp Trp Phe Ala Glu Asn Gly Glu Thr

465 470 475 480

Gly Asp Asp Glu

<210>71

<211>1819

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1789)

<223>RXA02445

<400>71

tgtataaagt gacgctctca gtgtagaaac tgagggtctc agtgaagtag actgacaggg 60

atacctaata cgtttaatag attgtggggc actcgccacc gtg gaa cca ttc gaa 115

Val Glu Pro Phe Glu

1 5

tta gag aaa gac ctt gag cgt ctt agg aaa aac gga aaa gac gat gaa 163

Leu Glu Lys Asp Leu Glu Arg Leu Arg Lys Asn Gly Lys Asp Asp Glu

10 15 20

acc gta gaa gtg aaa tct tgg ggt cgg tta cct tta agc aaa gga tca 211

Thr Val Glu Val Lys Ser Trp Gly Arg Leu Pro Leu Ser Lys Gly Ser

25 30 35

aaa agc ttc tgg gaa tca tta agc gca ttc gca aac acc aac ggt gga 259

Lys Ser Phe Trp Glu Ser Leu Ser Ala Phe Ala Asn Thr Asn Gly Gly

40 45 50

tac atc cta ttg ggg cta agc gaa cca gat ttc act cca gtt gaa gga 307

Tyr Ile Leu Leu Gly Leu Ser Glu Pro Asp Phe Thr Pro Val Glu Gly

55 60 65

ttt gat tca cag gcg agt atc cag ttc att cgt gca ggt tta aat cca 355

Phe Asp Ser Gln Ala Ser Ile Gln Phe Ile Arg Ala Gly Leu Asn Pro

70 75 80 85

caa gat cgc gac gcc caa aaa gtg gaa cca gtg ccc cat cat gaa att 403

Gln Asp Arg Asp Ala Gln Lys Val Glu Pro Val Pro His His Glu Ile

90 95 100

cat gaa atg act gtt gat ggt gct gaa gtt gtt tta gtt tca gtc tca 451

His Glu Met Thr Val Asp Gly Ala Glu Val Val Leu Val Ser Val Ser

105 110 115

ccg ttg tca gtg aac ggg ccc tgt tat tat ctt ccc gtc gga atc act 499

Pro Leu Ser Val Asn Gly Pro Cys Tyr Tyr Leu Pro Val Gly Ile Thr

120 125 130

aat ggc agc ttc aaa cgc gtt ggc gat gaa gac cgg aag ctc agt cat 547

Asn Gly Ser Phe Lys Arg Val Gly Asp Glu Asp Arg Lys Leu Ser His

135 140 145

ctt gaa att tac gag ctc caa aat agg ttt gtt caa acc aaa aca gat 595

Leu Glu Ile Tyr Glu Leu Gln Asn Arg Phe Val Gln Thr Lys Thr Asp

150 155 160 165

aga aat cca gtt cca gat tca agc atc gac gat ctc aac aat cag ctc 643

Arg Asn Pro Val Pro Asp Ser Ser Ile Asp Asp Leu Asn Asn Gln Leu

170 175 180

gcg gcg tca ttt aag cag cgc cta att gag tca aat agt cgc tcc ctt 691

Ala Ala Ser Phe Lys Gln Arg Leu Ile Glu Ser Asn Ser Arg Ser Leu

185 190 195

gga aca gac gat aac tgg tta ctg cgc aaa aat atc act aca tca aag 739

Gly Thr Asp Asp Asn Trp Leu Leu Arg Lys Asn Ile Thr Thr Ser Lys

200 205 210

gga gaa ctg acg att gct ggc tta ctg gct ctc gga agc tat cct caa 787

Gly Glu Leu Thr Ile Ala Gly Leu Leu Ala Leu Gly Ser Tyr Pro Gln

215 220 225

cag ttt ttc ccc cga gtg atc att gat gtt gcc gta cat cca ggt ctg 835

Gln Phe Phe Pro Arg Val Ile Ile Asp Val Ala Val His Pro Gly Leu

230 235 240 245

cat aag tca cca atc ggt acc tca att cgt ttt gaa gac cga aaa atc 883

His Lys Ser Pro Ile Gly Thr Ser Ile Arg Phe Glu Asp Arg Lys Ile

250 255 260

tgc gag gga aat ctt ctc gag atg gtt caa gag gct atg tct gcc atc 931

Cys Glu Gly Asn Leu Leu Glu Met Val Gln Glu Ala Met Ser Ala Ile

265 270 275

aaa cga aac cta cgt gta cgc cgc gtc gtt gaa gga ctc tca ggt aaa 979

Lys Arg Asn Leu Arg Val Arg Arg Val Val Glu Gly Leu Ser Gly Lys

280 285 290

gat gtt cta gaa atc cca gaa gaa gtt ttg aga gag gct cta gca aac 1027

Asp Val Leu Glu Ile Pro Glu Glu Val Leu Arg Glu Ala Leu Ala Asn

295 300 305

gcc gta ctt cac cgt gat tat tct gag cta gct caa aat gaa gca att 1075

Ala Val Leu His Arg Asp Tyr Ser Glu Leu Ala Gln Asn Glu Ala Ile

310 315 320 325

cat gta gac atc tat aag gat cga gtt gag atc acg agt cca ggt gga 1123

His Val Asp Ile Tyr Lys Asp Arg Val Glu Ile Thr Ser Pro Gly Gly

330 335 340

tta ccc aat ggt aaa cgc cca gag tca ata ctg gac gga tac tct gaa 1171

Leu Pro Asn Gly Lys Arg Pro Glu Ser Ile Leu Asp Gly Tyr Ser Glu

345 350 355

cca aga aat cgt gtg ctt tca aga atc cta atg gat att cca tgg aca 1219

Pro Arg Asn Arg Val Leu Ser Arg Ile Leu Met Asp Ile Pro Trp Thr

360 365 370

cat gaa gta caa gga gta ctt gct gaa agc aac ggt act ggc gtt ccc 1267

His Glu Val Gln Gly Val Leu Ala Glu Ser Asn Gly Thr Gly Val Pro

375 380 385

cga atg ttc aat ttg atg cgt gaa gcg gga ctt ccg gta ccg aat ttt 1315

Arg Met Phe Asn Leu Met Arg Glu Ala Gly Leu Pro Val Pro Asn Phe

390 395 400 405

aag att gat att tct agc gtc act gtc gaa ctc agc cgt cac ggt ctt 1363

Lys Ile Asp Ile Ser Ser Val Thr Val Glu Leu Ser Arg His Gly Leu

410 415 420

cta gat gcc caa aca agt gaa tgg ctt gta gaa aaa ctc gga tca gat 1411

Leu Asp Ala Gln Thr Ser Glu Trp Leu Val Glu Lys Leu Gly Ser Asp

425 430 435

ttt tct aac aca caa ggc att gct ctt gtt ctc gca aaa gaa ctt gga 1459

Phe Ser Asn Thr Gln Gly Ile Ala Leu Val Leu Ala Lys Glu Leu Gly

440 445 450

gcg gta acg tct cga gat ctc cgc aat caa act ggt cat gat tca gaa 1507

Ala Val Thr Ser Arg Asp Leu Arg Asn Gln Thr Gly His Asp Ser Glu

455 460 465

gac atg cgc agc tta ctt gac gct ttg gtt gat cgg ggc gtt cta aac 1555

Asp Met Arg Ser Leu Leu Asp Ala Leu Val Asp Arg Gly Val Leu Asn

470 475 480 485

caa aac tta cag aac caa tat cag ctt gcg aca tcg tct gtg aat gta 1603

Gln Asn Leu Gln Asn Gln Tyr Gln Leu Ala Thr Ser Ser Val Asn Val

490 495 500

act caa agc gaa caa gaa gtc tta gat gca atc aat aaa aca act cct 1651

Thr Gln Ser Glu Gln Glu Val Leu Asp Ala Ile Asn Lys Thr Thr Pro

505 510 515

gtc aca att cga gaa att gcc aca aaa aca ggg aaa act gca tcg tct 1699

Val Thr Ile Arg Glu Ile Ala Thr Lys Thr Gly Lys Thr Ala Ser Ser

520 525 530

ctt cgg ccg ctg ctt cgt ggc ctt gtt gaa gca ggt ctt gtg gtt gca 1747

Leu Arg Pro Leu Leu Arg Gly Leu Val Glu Ala Gly Leu Val Val Ala

535 540 545

act gct cca cca tca agc cgc aac cga gcg tac ttg aag gct 1789

Thr Ala Pro Pro Ser Ser Arg Asn Arg Ala Tyr Leu Lys Ala

550 555 560

tgacccacca acgaactcac cggtgtcagc 1819

<210>72

<211>563

<212>PRT

<213>谷氨酸棒状杆菌

<400>72

Val Glu Pro Phe Glu Leu Glu Lys Asp Leu Glu Arg Leu Arg Lys Asn

1 5 10 15

Gly Lys Asp Asp Glu Thr Val Glu Val Lys Ser Trp Gly Arg Leu Pro

20 25 30

Leu Ser Lys Gly Ser Lys Ser Phe Trp Glu Ser Leu Ser Ala Phe Ala

35 40 45

Asn Thr Asn Gly Gly Tyr Ile Leu Leu Gly Leu Ser Glu Pro Asp Phe

50 55 60

Thr Pro Val Glu Gly Phe Asp Ser Gln Ala Ser Ile Gln Phe Ile Arg

65 70 75 80

Ala Gly Leu Asn Pro Gln Asp Arg Asp Ala Gln Lys Val Glu Pro Val

85 90 95

Pro His His Glu Ile His Glu Met Thr Val Asp Gly Ala Glu Val Val

100 105 110

Leu Val Ser Val Ser Pro Leu Ser Val Asn Gly Pro Cys Tyr Tyr Leu

115 120 125

Pro Val Gly Ile Thr Asn Gly Ser Phe Lys Arg Val Gly Asp Glu Asp

130 135 140

Arg Lys Leu Ser His Leu Glu Ile Tyr Glu Leu Gln Asn Arg Phe Val

145 150 155 160

Gln Thr Lys Thr Asp Arg Asn Pro Val Pro Asp Ser Ser Ile Asp Asp

165 170 175

Leu Asn Asn Gln Leu Ala Ala Ser Phe Lys Gln Arg Leu Ile Glu Ser

180 185 190

Asn Ser Arg Ser Leu Gly Thr Asp Asp Asn Trp Leu Leu Arg Lys Asn

195 200 205

Ile Thr Thr Ser Lys Gly Glu Leu Thr Ile Ala Gly Leu Leu Ala Leu

210 215 220

Gly Ser Tyr Pro Gln Gln Phe Phe Pro Arg Val Ile Ile Asp Val Ala

225 230 235 240

Val His Pro Gly Leu His Lys Ser Pro Ile Gly Thr Ser Ile Arg Phe

245 250 255

Glu Asp Arg Lys Ile Cys Glu Gly Asn Leu Leu Glu Met Val Gln Glu

260 265 270

Ala Met Ser Ala Ile Lys Arg Asn Leu Arg Val Arg Arg Val Val Glu

275 280 285

Gly Leu Ser Gly Lys Asp Val Leu Glu Ile Pro Glu Glu Val Leu Arg

290 295 300

Glu Ala Leu Ala Asn Ala Val Leu His Arg Asp Tyr Ser Glu Leu Ala

305 310 315 320

Gln Asn Glu Ala Ile His Val Asp Ile Tyr Lys Asp Arg Val Glu Ile

325 330 335

Thr Ser Pro Gly Gly Leu Pro Asn Gly Lys Arg Pro Glu Ser Ile Leu

340 345 350

Asp Gly Tyr Ser Glu Pro Arg Asn Arg Val Leu Ser Arg Ile Leu Met

355 360 365

Asp Ile Pro Trp Thr His Glu Val Gln Gly Val Leu Ala Glu Ser Asn

370 375 380

Gly Thr Gly Val Pro Arg Met Phe Asn Leu Met Arg Glu Ala Gly Leu

385 390 395 400

Pro Val Pro Asn Phe Lys Ile Asp Ile Ser Ser Val Thr Val Glu Leu

405 410 415

Ser Arg His Gly Leu Leu Asp Ala Gln Thr Ser Glu Trp Leu Val Glu

420 425 430

Lys Leu Gly Ser Asp Phe Ser Asn Thr Gln Gly Ile Ala Leu Val Leu

435 440 445

Ala Lys Glu Leu Gly Ala Val Thr Ser Arg Asp Leu Arg Asn Gln Thr

450 455 460

Gly His Asp Ser Glu Asp Met Arg Ser Leu Leu Asp Ala Leu Val Asp

465 470 475 480

Arg Gly Val Leu Asn Gln Asn Leu Gln Asn Gln Tyr Gln Leu Ala Thr

485 490 495

Ser Ser Val Asn Val Thr Gln Ser Glu Gln Glu Val Leu Asp Ala Ile

500 505 510

Asn Lys Thr Thr Pro Val Thr Ile Arg Glu Ile Ala Thr Lys Thr Gly

515 520 525

Lys Thr Ala Ser Ser Leu Arg Pro Leu Leu Arg Gly Leu Val Glu Ala

530 535 540

Gly Leu Val Val Ala Thr Ala Pro Pro Ser Ser Arg Asn Arg Ala Tyr

545 550 555 560

Leu Lys Ala

<210>73

<211>1009

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(979)

<223>RXA02476

<400>73

cgggcggagt tctatcaaca ttacgcaaag gcataagctt tattattcca ctcggtgtga 60

catatgacct aaagtgccag tcagtacaat catttaggtc atg tca ttt aca gct 115

Met Ser Phe Thr Ala

1 5

ttt caa aca gcc ctg ctc gtg tgg ttt aga gca aat gcc cgc gat ctt 163

Phe Gln Thr Ala Leu Leu Val Trp Phe Arg Ala Asn Ala Arg Asp Leu

10 15 20

gcg tgg cgt gat ccc aat act tca gca tgg gga att ctc ctt tca gag 211

Ala Trp Arg Asp Pro Asn Thr Ser Ala Trp Gly Ile Leu Leu Ser Glu

25 30 35

gtg atg agc caa caa act ccc gtc gcg cga gtc gag ccg att tgg cgt 259

Val Met Ser Gln Gln Thr Pro Val Ala Arg Val Glu Pro Ile Trp Arg

40 45 50

gag tgg atg gaa aaa tgg ccc act ccg gaa gat ttc gcg aat gcg agc 307

Glu Trp Met Glu Lys Trp Pro Thr Pro Glu Asp Phe Ala Asn Ala Ser

55 60 65

acc gat gag att ttg cgg tcg tgg ggc aag ttg ggc tat cca cgt agg 355

Thr Asp Glu Ile Leu Arg Ser Trp Gly Lys Leu Gly Tyr Pro Arg Arg

70 75 80 85

gcg ctg agg ttg aag gaa tgt gcg gag gtg atc gtc gaa aag cat gcc 403

Ala Leu Arg Leu Lys Glu Cys Ala Glu Val Ile Val Glu Lys His Ala

90 95 100

ggc gag gtg ccg gat acg gtg gag gcg ctg ctc gcg ttg ccg ggg atc 451

Gly Glu Val Pro Asp Thr Val Glu Ala Leu Leu Ala Leu Pro Gly Ile

105 110 115

ggt gat tac acg gcg cgc gcg gtc gcg gcg ttt cat ttt ggg cag cgc 499

Gly Asp Tyr Thr Ala Arg Ala Val Ala Ala Phe His Phe Gly Gln Arg

120 125 130

gtg ccg gtg gtc gat acg aac gtg cgt cgc gtg tac cag cgc gcg gta 547

Val Pro Val Val Asp Thr Asn Val Arg Arg Val Tyr Gln Arg Ala Val

135 140 145

gcc gga cgt tac ctt gcg ggg cct gcg aaa aag caa gag ctt atc gac 595

Ala Gly Arg Tyr Leu Ala Gly Pro Ala Lys Lys Gln Glu Leu Ile Asp

150 155 160 165

gtc tcc ctt ctc ctt ccc aac act cac gcc cca gaa ttc tct gcc gca 643

Val Ser Leu Leu Leu Pro Asn Thr His Ala Pro Glu Phe Ser Ala Ala

170 175 180

ata atg gag ttg ggt gct ctt atc tgc acg gcc act tcc cca aag tgt 691

Ile Met Glu Leu Gly Ala Leu Ile Cys Thr Ala Thr Ser Pro Lys Cys

185 190 195

gac acc tgc cca ctg ctt gac cag tgt caa tgg caa aaa ctt ggc tgt 739

Asp Thr Cys Pro Leu Leu Asp Gln Cys Gln Trp Gln Lys Leu Gly Cys

200 205 210

ccc tcc ccg agt gaa gag gag ctg gct tca gcg aaa aag cgt gtg cag 787

Pro Ser Pro Ser Glu Glu Glu Leu Ala Ser Ala Lys Lys Arg Val Gln

215 220 225

aaa ttt gtg gga acc gac cga caa gtc cgt ggc cta atc atg gac gta 835

Lys Phe Val Gly Thr Asp Arg Gln Val Arg Gly Leu Ile Met Asp Val

230 235 240 245

ctg cgc aat gcc acc gca cct gtg cca cta tcc gcg att gat gtc gtg 883

Leu Arg Asn Ala Thr Ala Pro Val Pro Leu Ser Ala Ile Asp Val Val

250 255 260

tgg cct gac gat gcc caa cgc tcc cgg gcg ctg ttt tcg ctc att gag 931

Trp Pro Asp Asp Ala Gln Arg Ser Arg Ala Leu Phe Ser Leu Ile Glu

265 270 275

gac gga ctc gcg gaa caa aat gag gcg ggt tat ttc cac ctg cca cgg 979

Asp Gly Leu Ala Glu Gln Asn Glu Ala Gly Tyr Phe His Leu Pro Arg

280 285 290

taaaccactg cgcgcctgca aaaaacagta 1009

<210>74

<211>293

<212>PRT

<213>谷氨酸棒状杆菌

<400>74

Met Ser Phe Thr Ala Phe Gln Thr Ala Leu Leu Val Trp Phe Arg Ala

1 5 10 15

Asn Ala Arg Asp Leu Ala Trp Arg Asp Pro Asn Thr Ser Ala Trp Gly

20 25 30

Ile Leu Leu Ser Glu Val Met Ser Gln Gln Thr Pro Val Ala Arg Val

35 40 45

Glu Pro Ile Trp Arg Glu Trp Met Glu Lys Trp Pro Thr Pro Glu Asp

50 55 60

Phe Ala Asn Ala Ser Thr Asp Glu Ile Leu Arg Ser Trp Gly Lys Leu

65 70 75 80

Gly Tyr Pro Arg Arg Ala Leu Arg Leu Lys Glu Cys Ala Glu Val Ile

85 90 95

Val Glu Lys His Ala Gly Glu Val Pro Asp Thr Val Glu Ala Leu Leu

100 105 110

Ala Leu Pro Gly Ile Gly Asp Tyr Thr Ala Arg Ala Val Ala Ala Phe

115 120 125

His Phe Gly Gln Arg Val Pro Val Val Asp Thr Asn Val Arg Arg Val

130 135 140

Tyr Gln Arg Ala Val Ala Gly Arg Tyr Leu Ala Gly Pro Ala Lys Lys

145 150 155 160

Gln Glu Leu Ile Asp Val Ser Leu Leu Leu Pro Asn Thr His Ala Pro

165 170 175

Glu Phe Ser Ala Ala Ile Met Glu Leu Gly Ala Leu Ile Cys Thr Ala

180 185 190

Thr Ser Pro Lys Cys Asp Thr Cys Pro Leu Leu Asp Gln Cys Gln Trp

195 200 205

Gln Lys Leu Gly Cys Pro Ser Pro Ser Glu Glu Glu Leu Ala Ser Ala

210 215 220

Lys Lys Arg Val Gln Lys Phe Val Gly Thr Asp Arg Gln Val Arg Gly

225 230 235 240

Leu Ile Met Asp Val Leu Arg Asn Ala Thr Ala Pro Val Pro Leu Ser

245 250 255

Ala Ile Asp Val Val Trp Pro Asp Asp Ala Gln Arg Ser Arg Ala Leu

260 265 270

Phe Ser Leu Ile Glu Asp Gly Leu Ala Glu Gln Asn Glu Ala Gly Tyr

275 280 285

Phe His Leu Pro Arg

290

<210>75

<211>3319

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3289)

<223>RXA02726

<400>75

ttgtgtcaac gaagtggagc tagttaattt agctcaagct gggtggtacc gcgtccgttt 60

tttagggcgt ccccgcaggt agaacgataa ttattgttac ttg cgt gaa gga tgg 115

Leu Arg Glu Gly Trp

1 5

gac cga aca cac atg tct gaa gcc gtt ggc gga gtt tac cca cag gtt 163

Asp Arg Thr His Met Ser Glu Ala Val Gly Gly Val Tyr Pro Gln Val

10 15 20

gat tta tct ggt ggg tca tcc aga ttt cca gag atg gaa gag aat gta 211

Asp Leu Ser Gly Gly Ser Ser Arg Phe Pro Glu Met Glu Glu Asn Val

25 30 35

ctg agc tac tgg aag aag gat gac acc ttc cag gcc agc atc gat cag 259

Leu Ser Tyr Trp Lys Lys Asp Asp Thr Phe Gln Ala Ser Ile Asp Gln

40 45 50

cgc gat ggt gct gaa gac tac gtc ttt tac gat ggc cct cct ttt gca 307

Arg Asp Gly Ala Glu Asp Tyr Val Phe Tyr Asp Gly Pro Pro Phe Ala

55 60 65

aac ggt ctg cca cac tac ggc cac cta ctg act ggt tac gtc aag gac 355

Asn Gly Leu Pro His Tyr Gly His Leu Leu Thr Gly Tyr Val Lys Asp

70 75 80 85

att gtt cct cgc tac cag acc atg cgt ggc tac cgc gtt cct cgt gtc 403

Ile Val Pro Arg Tyr Gln Thr Met Arg Gly Tyr Arg Val Pro Arg Val

90 95 100

ttc ggt tgg gat acc cac ggt ctg cca gct gaa ctt gag gct gaa aag 451

Phe Gly Trp Asp Thr His Gly Leu Pro Ala Glu Leu Glu Ala Glu Lys

105 110 115

cag ctc ggc atc aag gac aag ggc gag atc gag gcc atg ggt ctt gcc 499

Gln Leu Gly Ile Lys Asp Lys Gly Glu Ile Glu Ala Met Gly Leu Ala

120 125 130

aag ttc aac gag tac tgt gca acc tcc gtg ttg cag tac acc aag gaa 547

Lys Phe Asn Glu Tyr Cys Ala Thr Ser Val Leu Gln Tyr Thr Lys Glu

135 140 145

tgg gaa gag tac gtc acc cgc cag gct cgt tgg gtg gac ttt gaa aac 595

Trp Glu Glu Tyr Val Thr Arg Gln Ala Arg Trp Val Asp Phe Glu Asn

150 155 160 165

ggc tac aag acc atg gac ctt tct ttc atg gag tcc gtg atc tgg gcg 643

Gly Tyr Lys Thr Met Asp Leu Ser Phe Met Glu Ser Val Ile Trp Ala

170 175 180

ttc aag gaa ctc tac gac aag ggc ctg atc tac cag ggt ttc cgc gtt 691

Phe Lys Glu Leu Tyr Asp Lys Gly Leu Ile Tyr Gln Gly Phe Arg Val

185 190 195

ctt cct tac tcc tgg gca gag cac acc cca ctg tcc aac cag gaa acc 739

Leu Pro Tyr Ser Trp Ala Glu His Thr Pro Leu Ser Asn Gln Glu Thr

200 205 210

cga ctg gat gac tcc tac aag ctg cgc cag gat cca acc ctg acc gtc 787

Arg Leu Asp Asp Ser Tyr Lys Leu Arg Gln Asp Pro Thr Leu Thr Val

215 220 225

acg ttc cca gtc acc ggt gtc gtc gaa ggt tct tct gca aac gct ggc 835

Thr Phe Pro Val Thr Gly Val Val Glu Gly Ser Ser Ala Asn Ala Gly

230 235 240 245

ctg gtg gga gcg ttg gct ctt gcg tgg acg act acc ccg tgg acc ctt 883

Leu Val Gly Ala Leu Ala Leu Ala Trp Thr Thr Thr Pro Trp Thr Leu

250 255 260

cca tcc aac ctt gcg ttg gct gtg aac cca gcg gtg acc tac gca ttg 931

Pro Ser Asn Leu Ala Leu Ala Val Asn Pro Ala Val Thr Tyr Ala Leu

265 270 275

gtt gag gtt gct gaa gac ggt gag gca gaa ttc gtc ggc aag cgt gtg 979

Val Glu Val Ala Glu Asp Gly Glu Ala Glu Phe Val Gly Lys Arg Val

280 285 290

ctt ttg gct aag gac ctc gtt ggt tcc tac gcc aag gaa ctc ggt gct 1027

Leu Leu Ala Lys Asp Leu Val Gly Ser Tyr Ala Lys Glu Leu Gly Ala

295 300 305

gag gct gtt atc gtt tct gag cac cca ggc tct gaa ctg gtc gga ctg 1075

Glu Ala Val Ile Val Ser Glu His Pro Gly Ser Glu Leu Val Gly Leu

310 315 320 325

acc tac gag cca atc ttt gga tat ttc cgc gat cac gcg aac gga ttc 1123

Thr Tyr Glu Pro Ile Phe Gly Tyr Phe Arg Asp His Ala Asn Gly Phe

330 335 340

cag atc ctc ggt gca gag tac gtc acc acc gaa gac ggc acc ggt atc 1171

Gln Ile Leu Gly Ala Glu Tyr Val Thr Thr Glu Asp Gly Thr Gly Ile

345 350 355

gtc cac cag gca cca gct ttc ggt gaa gac gat atg aac acc tgt aac 1219

Val His Gln Ala Pro Ala Phe Gly Glu Asp Asp Met Asn Thr Cys Asn

360 365 370

gct gcc ggc att gag cca gtc atc cca gtg gac atc gac ggc aag ttc 1267

Ala Ala Gly Ile Glu Pro Val Ile Pro Val Asp Ile Asp Gly Lys Phe

375 380 385

acc ggt ttg gtt cct gaa tac caa ggt cag ctt gtt ttc gat gcc aac 1315

Thr Gly Leu Val Pro Glu Tyr Gln Gly Gln Leu Val Phe Asp Ala Asn

390 395 400 405

aag gac atc atc aag gac ttg aag gct gca ggt cgc gtg gtt cgc cac 1363

Lys Asp Ile Ile Lys Asp Leu Lys Ala Ala Gly Arg Val Val Arg His

410 415 420

cag acc atc gaa cac tcc tac cca cac tct tgg cgt tcc ggt gag cca 1411

Gln Thr Ile Glu His Ser Tyr Pro His Ser Trp Arg Ser Gly Glu Pro

425 430 435

ctg atc tac atg gct ctg cca tct tgg ttt gtg aat gtc acc gaa atc 1459

Leu Ile Tyr Met Ala Leu Pro Ser Trp Phe Val Asr Val Thr Glu Ile

440 445 450

cgc gac cgc atg gtt gag gtc aac cag gac atc gag tgg atg cca gcg 1507

Arg Asp Arg Met Val Glu Val Asn Gln Asp Ile Glu Trp Met Pro Ala

455 460 465

cac atc cgc gac ggc cag ttc ggc aag tgg cta gaa ggt gcc cgc gac 1555

His Ile Arg Asp Gly Gln Phe Gly Lys Trp Leu Glu Gly Ala Arg Asp

470 475 480 485

tgg aac atc tcc cgt tcc cgt tac tgg ggt tca cca att cca gca tgg 1603

Trp Asr Ile Ser Arg Ser Arg Tyr Trp Gly Ser Pro Ile Pro Ala Trp

490 495 500

gtc tcc gac aac gac gaa tac cca cgc gtt gat gtt tat ggt tcc ctc 1651

Val Ser Asp Asn Asp Glu Tyr Pro Arg Val Asp Val Tyr Gly Ser Leu

505 510 515

gat gag ctt gag gct gac ttt ggc gtg cgt cca aag tcc ctg cac cgt 1699

Asp Glu Leu Glu Ala Asp Phe Gly Val Arg Pro Lys Ser Leu His Arg

520 525 530

cca gac atc gat gaa cta act cgt cca aac cca gac gat cca acc ggc 1747

Pro Asp Ile Asp Glu Leu Thr Arg Pro Asn Pro Asp Asp Pro Thr Gly

535 540 545

aag tcc acc atg cga cgc gtc acc gat gtt ttg gac gtg tgg ttc gac 1795

Lys Ser Thr Met Arg Arg Val Thr Asp Val Leu Asp Val Trp Phe Asp

550 555 560 565

tcc ggt tcc atg ccg ttt gcc cag gtg cac tac cca ttc gag aac aaa 1843

Ser Gly Ser Met Pro Phe Ala Gln Val His Tyr Pro Phe Glu Asn Lys

570 575 580

gaa tgg ttt gat acc cac gca cca gca gac ttc atc gtg gag tac atc 1891

Glu Trp Phe Asp Thr His Ala Pro Ala Asp Phe Ile Val Glu Tyr Ile

585 590 595

ggt cag acc cgc ggt tgg ttc tac ctg ctg cac gtg ctg tcc acc gca 1939

Gly Gln Thr Arg Gly Trp Phe Tyr Leu Leu His Val Leu Ser Thr Ala

600 605 610

ctg ttt gac cgc cca gct ttc aag aag gtt gtc gca cac ggc atc gtc 1987

Leu Phe Asp Arg Pro Ala Phe Lys Lys Val Val Ala His Gly Ile Val

615 620 625

ttg ggt gat gac gga ctg aag atg tcc aag tcc aag ggc aac tac ccg 2035

Leu Gly Asp Asp Gly Leu Lys Met Ser Lys Ser Lys Gly Asn Tyr Pro

630 635 640 645

aac gtc aac gag gtc ttc gac cgc gac ggt tcc gac gcc atg cgt tgg 2083

Asn Val Asn Glu Val Phe Asp Arg Asp Gly Ser Asp Ala Met Arg Trp

650 655 660

ttc ctc atg agt tcc cca atc ctg cgc ggc ggc aac ttg att gtc acc 2131

Phe Leu Met Ser Ser Pro Ile Leu Arg Gly Gly Asn Leu Ile Val Thr

665 670 675

gaa aag ggc atc cgc gaa ggt gtg cgc caa gca cag ctt cca atg tgg 2179

Glu Lys Gly Ile Arg Glu Gly Val Arg Gln Ala Gln Leu Pro Met Trp

680 685 690

aac gca tac tcc ttc ctg cag ctg tac acc tcc aag aac gca acc tgg 2227

Asn Ala Tyr Ser Phe Leu Gln Leu Tyr Thr Ser Lys Asn Ala Thr Trp

695 700 705

tca gtc gac tcc act gac gtg ctg gac cgc tac atc ctg gcg aag ctg 2275

Ser Val Asp Ser Thr Asp Val Leu Asp Arg Tyr Ile Leu Ala Lys Leu

710 715 720 725

cac gat ttg gtg gca gag acc cag gcg gca ctc gac ggc act gac att 2323

His Asp Leu Val Ala Glu Thr Gln Ala Ala Leu Asp Gly Thr Asp Ile

730 735 740

gca aag gct tgc gac ttg gtt cgt aac ttc tgt gat gcg ttg acc aac 2371

Ala Lys Ala Cys Asp Leu Val Arg Asn Phe Cys Asp Ala Leu Thr Asn

745 750 755

tgg tac gtg cgt cgt tcc cgc gac cgt ttc tgg gct ggt gat gaa gca 2419

Trp Tyr Val Arg Arg Ser Arg Asp Arg Phe Trp Ala Gly Asp Glu Ala

760 765 770

cac cca gag gct ttc aac acc ttg tac acc gtg ctg gaa acc ctc acc 2467

His Pro Glu Ala Phe Asn Thr Leu Tyr Thr Val Leu Glu Thr Leu Thr

775 780 785

cgc gtg gca gct cca ctg ctg cca atg acc acc gaa gtg atc tgg cgt 2515

Arg Val Ala Ala Pro Leu Leu Pro Met Thr Thr Glu Val Ile Trp Arg

790 795 800 805

gga ctg acc ggc gag cgt tct gtg cac ctg act gat ttc cca tcc gct 2563

Gly Leu Thr Gly Glu Arg Ser Val His Leu Thr Asp Phe Pro Ser Ala

810 815 820

gag tct ttc cca gca gat gct gat ttg gtt cgc acc atg gat gag atc 2611

Glu Ser Phe Pro Ala Asp Ala Asp Leu Val Arg Thr Met Asp Glu Ile

825 830 835

cgt ggc gtg tgc tct gcg gct tcc tct gtt cgt aag gct cac aag ctg 2659

Arg Gly Val Cys Ser Ala Ala Ser Ser Val Arg Lys Ala His Lys Leu

840 845 850

cgt aac cgt ctg cca ctt cca ggc ctg act gtt gct ctt cca gac tct 2707

Arg Asn Arg Leu Pro Leu Pro Gly Leu Thr Val Ala Leu Pro Asp Ser

855 860 865

gct cgc ctg gca gac ttc gct tcg atc atc cgc gat gag gtc aac gtg 2755

Ala Arg Leu Ala Asp Phe Ala Ser Ile Ile Arg Asp Glu Val Asn Val

870 875 880 885

aag aac gtg gat ctg acc tct gac gtg gat tcc gtg gga acc ttc gag 2803

Lys Asn Val Asp Leu Thr Ser Asp Val Asp Ser Val Gly Thr Phe Glu

890 895 900

gtt gtt gtt aac gct aag gtt gca ggt cct cgc ttg ggc aag gac gtc 2851

Val Val Val Asn Ala Lys Val Ala Gly Pro Arg Leu Gly Lys Asp Val

905 910 915

cag cgc gtg atc aag gct gtg aag gct ggc aac tac acc cgc gaa ggc 2899

Gln Arg Val Ile Lys Ala Val Lys Ala Gly Asn Tyr Thr Arg Glu Gly

920 925 930

gac gtc gtt gtt gcc gat ggc atc gag ctc aac gag ggt gaa ttc acc 2947

Asp Val Val Val Ala Asp Gly Ile Glu Leu Asn Glu Gly Glu Phe Thr

935 940 945

gag cgt ctc gta gca gca aac cct gat tcc acc gcg cag atc gac ggc 2995

Glu Arg Leu Val Ala Ala Asn Pro Asp Ser Thr Ala Gln Ile Asp Gly

950 955 960 965

gtg gat gga ctc gtg gtt ctg gac atg gaa gtc acg gaa gaa ctt gaa 3043

Val Asp Gly Leu Val Val Leu Asp Met Glu Val Thr Glu Glu Leu Glu

970 975 980

gca gaa ggc tgg gca gcg gac gcg atc cgt ggc ctg cag gat gct cga 3091

Ala Glu Gly Trp Ala Ala Asp Ala Ile Arg Gly Leu Gln Asp Ala Arg

985 990 995

aag aac tcc ggc ttt gag gtt tct gac cgc att tct gtt gtc gtc agc 3139

Lys Asn Ser Gly Phe Glu Val Ser Asp Arg Ile Ser Val Val Val Ser

1000 1005 1010

gtt cct gag gac aag aag gaa tgg atc acc act cac gct gat cac atc 3187

Val Pro Glu Asp Lys Lys Glu Trp Ile Thr Thr His Ala Asp His Ile

1015 1020 1025

gca gcg gaa gtt ttg gca acc tcc ttt gag atc gtc act gat gcc ctc 3235

Ala Ala Glu Val Leu Ala Thr Ser Phe Glu Ile Val Thr Asp Ala Leu

1030 1035 1040 1045

gac ggc gaa acc cac gac att gtc gct ggt gtg acc gcg aag gtt act 3283

Asp Gly Glu Thr His Asp Ile Val Ala Gly Val Thr Ala Lys Val Thr

1050 1055 1060

aag aac taagagttgt tttgttgaga aagcccgctg 3319

Lys Asn

<210>76

<211>1063

<212>PRT

<213>谷氨酸棒状杆菌

<400>76

Leu Arg Glu Gly Trp Asp Arg Thr His Met Ser Glu Ala Val Gly Gly

1 5 10 15

Val Tyr Pro Gln Val Asp Leu Ser Gly Gly Ser Ser Arg Phe Pro Glu

20 25 30

Met Glu Glu Asn Val Leu Ser Tyr Trp Lys Lys Asp Asp Thr Phe Gln

35 40 45

Ala Ser Ile Asp Gln Arg Asp Gly Ala Glu Asp Tyr Val Phe Tyr Asp

50 55 60

Gly Pro Pro Phe Ala Asn Gly Leu Pro His Tyr Gly His Leu Leu Thr

65 70 75 80

Gly Tyr Val Lys Asp Ile Val Pro Arg Tyr Gln Thr Met Arg Gly Tyr

85 90 95

Arg Val Pro Arg Val Phe Gly Trp Asp Thr His Gly Leu Pro Ala Glu

100 105 110

Leu Glu Ala Glu Lys Gln Leu Gly Ile Lys Asp Lys Gly Glu Ile Glu

115 120 125

Ala Met Gly Leu Ala Lys Phe Asn Glu Tyr Cys Ala Thr Ser Val Leu

130 135 140

Gln Tyr Thr Lys Glu Trp Glu Glu Tyr Val Thr Arg Gln Ala Arg Trp

145 150 155 160

Val Asp Phe Glu Asn Gly Tyr Lys Thr Met Asp Leu Ser Phe Met Glu

165 170 175

Ser Val Ile Trp Ala Phe Lys Glu Leu Tyr Asp Lys Gly Leu Ile Tyr

180 185 190

Gln Gly Phe Arg Val Leu Pro Tyr Ser Trp Ala Glu His Thr Pro Leu

195 200 205

Ser Asn Gln Glu Thr Arg Leu Asp Asp Ser Tyr Lys Leu Arg Gln Asp

210 215 220

Pro Thr Leu Thr Val Thr Phe Pro Val Thr Gly Val Val Glu Gly Ser

225 230 235 240

Ser Ala Asn Ala Gly Leu Val Gly Ala Leu Ala Leu Ala Trp Thr Thr

245 250 255

Thr Pro Trp Thr Leu Pro Ser Asn Leu Ala Leu Ala Val Asn Pro Ala

260 265 270

Val Thr Tyr Ala Leu Val Glu Val Ala Glu Asp Gly Glu Ala Glu Phe

275 280 285

Val Gly Lys Arg Val Leu Leu Ala Lys Asp Leu Val Gly Ser Tyr Ala

290 295 300

Lys Glu Leu Gly Ala Glu Ala Val Ile Val Ser Glu His Pro Gly Ser

305 310 315 320

Glu Leu Val Gly Leu Thr Tyr Glu Pro Ile Phe Gly Tyr Phe Arg Asp

325 330 335

His Ala Asn Gly Phe Gln Ile Leu Gly Ala Glu Tyr Val Thr Thr Glu

340 345 350

Asp Gly Thr Gly Ile Val His Gln Ala Pro Ala Phe Gly Glu Asp Asp

355 360 365

Met Asn Thr Cys Asn Ala Ala Gly Ile Glu Pro Val Ile Pro Val Asp

370 375 380

Ile Asp Gly Lys Phe Thr Gly Leu Val Pro Glu Tyr Gln Gly Gln Leu

385 390 395 400

Val Phe Asp Ala Asn Lys Asp Ile Ile Lys Asp Leu Lys Ala Ala Gly

405 410 415

Arg Val Val Arg His Gln Thr Ile Glu His Ser Tyr Pro His Ser Trp

420 425 430

Arg Ser Gly Glu Pro Leu Ile Tyr Met Ala Leu Pro Ser Trp Phe Val

435 440 445

Asn Val Thr Glu Ile Arg Asp Arg Met Val Glu Val Asn Gln Asp Ile

450 455 460

Glu Trp Met Pro Ala His Ile Arg Asp Gly Gln Phe Gly Lys Trp Leu

465 470 475 480

Glu Gly Ala Arg Asp Trp Asn Ile Ser Arg Ser Arg Tyr Trp Gly Ser

485 490 495

Pro Ile Pro Ala Trp Val Ser Asp Asn Asp Glu Tyr Pro Arg Val Asp

500 505 510

Val Tyr Gly Ser Leu Asp Glu Leu Glu Ala Asp Phe Gly Val Arg Pro

515 520 525

Lys Ser Leu His Arg Pro Asp Ile Asp Glu Leu Thr Arg Pro Asn Pro

530 535 540

Asp Asp Pro Thr Gly Lys Ser Thr Met Arg Arg Val Thr Asp Val Leu

545 550 555 560

Asp Val Trp Phe Asp Ser Gly Ser Met Pro Phe Ala Gln Val His Tyr

565 570 575

Pro Phe Glu Asn Lys Glu Trp Phe Asp Thr His Ala Pro Ala Asp Phe

580 585 590

Ile Val Glu Tyr Ile Gly Gln Thr Arg Gly Trp Phe Tyr Leu Leu His

595 600 605

Val Leu Ser Thr Ala Leu Phe Asp Arg Pro Ala Phe Lys Lys Val Val

610 615 620

Ala His Gly Ile Val Leu Gly Asp Asp Gly Leu Lys Met Ser Lys Ser

625 630 635 640

Lys Gly Asn Tyr Pro Asn Val Asn Glu Val Phe Asp Arg Asp Gly Ser

645 650 655

Asp Ala Met Arg Trp Phe Leu Met Ser Ser Pro Ile Leu Arg Gly Gly

660 665 670

Asn Leu Ile Val Thr Glu Lys Gly Ile Arg Glu Gly Val Arg Gln Ala

675 680 685

Gln Leu Pro Met Trp Asn Ala Tyr Ser Phe Leu Gln Leu Tyr Thr Ser

690 695 700

Lys Asn Ala Thr Trp Ser Val Asp Ser Thr Asp Val Leu Asp Arg Tyr

705 710 715 720

Ile Leu Ala Lys Leu His Asp Leu Val Ala Glu Thr Gln Ala Ala Leu

725 730 735

Asp Gly Thr Asp Ile Ala Lys Ala Cys Asp Leu Val Arg Asn Phe Cys

740 745 750

Asp Ala Leu Thr Asn Trp Tyr Val Arg Arg Ser Arg Asp Arg Phe Trp

755 760 765

Ala Gly Asp Glu Ala His Pro Glu Ala Phe Asn Thr Leu Tyr Thr Val

770 775 780

Leu Glu Thr Leu Thr Arg Val Ala Ala Pro Leu Leu Pro Met Thr Thr

785 790 795 800

Glu Val Ile Trp Arg Gly Leu Thr Gly Glu Arg Ser Val His Leu Thr

805 810 815

Asp Phe Pro Ser Ala Glu Ser Phe Pro Ala Asp Ala Asp Leu Val Arg

820 825 830

Thr Met Asp Glu Ile Arg Gly Val Cys Ser Ala Ala Ser Ser Val Arg

835 840 845

Lys Ala His Lys Leu Arg Asn Arg Leu Pro Leu Pro Gly Leu Thr Val

850 855 860

Ala Leu Pro Asp Ser Ala Arg Leu Ala Asp Phe Ala Ser Ile Ile Arg

865 870 875 880

Asp Glu Val Asn Val Lys Asn Val Asp Leu Thr Ser Asp Val Asp Ser

885 890 895

Val Gly Thr Phe Glu Val Val Val Asn Ala Lys Val Ala Gly Pro Arg

900 905 910

Leu Gly Lys Asp Val Gln Arg Val Ile Lys Ala Val Lys Ala Gly Asn

915 920 925

Tyr Thr Arg Glu Gly Asp Val Val Val Ala Asp Gly Ile Glu Leu Asn

930 935 940

Glu Gly Glu Phe Thr Glu Arg Leu Val Ala Ala Asn Pro Asp Ser Thr

945 950 955 960

Ala Gln Ile Asp Gly Val Asp Gly Leu Val Val Leu Asp Met Glu Val

965 970 975

Thr Glu Glu Leu Glu Ala Glu Gly Trp Ala Ala Asp Ala Ile Arg Gly

980 985 990

Leu Gln Asp Ala Arg Lys Asn Ser Gly Phe Glu Val Ser Asp Arg Ile

995 1000 1005

Ser Val Val Val Ser Val Pro Glu Asp Lys Lys Glu Trp Ile Thr Thr

1010 1015 1020

His Ala Asp His Ile Ala Ala Glu Val Leu Ala Thr Ser Phe Glu Ile

1025 1030 1035 1040

Val Thr Asp Ala Leu Asp Gly Glu Thr His Asp Ile Val Ala Gly Val

1045 1050 1055

Thr Ala Lys Val Thr Lys Asn

1060

<210>77

<211>2290

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2260)

<223>RXA02731

<400>77

gttgcatcga gtccgcgggt ttcgcacgct tgatctaaat tcttgagggt tttccggccg 60

ttgtttgcgc taaacgtagg ggtcaagcgt cgaaaagcgc ttg ctt gca cgc tgt 115

Leu Leu Ala Arg Cys

1 5

ttt gct gcg ggc cgc aac gtg tcc acc ctg tgg cgt atc cta gaa tgc 163

Phe Ala Ala Gly Arg Asn Val Ser Thr Leu Trp Arg Ile Leu Glu Cys

10 15 20

atg gct ttt gct gct gaa cat cct gtc ctg tcc cac tct gag cac cgc 211

Met Ala Phe Ala Ala Glu His Pro Val Leu Ser His Ser Glu His Arg

25 30 35

ccg gtt ggt gaa atc gag cgt agc gat gac aaa ttt gtt gtc gtt agt 259

Pro Val Gly Glu Ile Glu Arg Ser Asp Asp Lys Phe Val Val Val Ser

40 45 50

gaa ttt gag cct gcg ggt gac cag cct gcg gct att aaa gag ctc gat 307

Glu Phe Glu Pro Ala Gly Asp Gln Pro Ala Ala Ile Lys Glu Leu Asp

55 60 65

gag cgc ttg gat cgc ggt gag cgg gac gtc gtt ttg atg ggt gct act 355

Glu Arg Leu Asp Arg Gly Glu Arg Asp Val Val Leu Met Gly Ala Thr

70 75 80 85

ggt acg ggt aag tcc gcg act gcg gcg tgg ttg atc gaa aag cag cag 403

Gly Thr Gly Lys Ser Ala Thr Ala Ala Trp Leu Ile Glu Lys Gln Gln

90 95 100

cgc ccc gct ttg gtg atg gcg ccg aat aag acg ctg gct gcg cag ttg 451

Arg Pro Ala Leu Val Met Ala Pro Asn Lys Thr Leu Ala Ala Gln Leu

105 110 115

gct aat gaa ttg cgg cag ctg ttg ccc aat aac gcg gtg gag tat ttc 499

Ala Asn Glu Leu Arg Gln Leu Leu Pro Asn Asn Ala Val Glu Tyr Phe

120 125 130

gtg tct tat tac gat tac tac cag cca gaa gcg tat atc gcg cag act 547

Val Ser Tyr Tyr Asp Tyr Tyr Gln Pro Glu Ala Tyr Ile Ala Gln Thr

135 140 145

gat acc tat att gaa aag gac tcc tcg att aat gag gat gtg gag cgt 595

Asp Thr Tyr Ile Glu Lys Asp Ser Ser Ile Asn Glu Asp Val Glu Arg

150 155 160 165

ctg cgt cac tcg gcg acg tcg tct ttg ctg agt agg cga gac gtc gtg 643

Leu Arg His Ser Ala Thr Ser Ser Leu Leu Ser Arg Arg Asp Val Val

170 175 180

gtt gtt agt tcg gtg tcg tgt att tat ggc ttg ggc act cca cag tct 691

Val Val Ser Ser Val Ser Cys Ile Tyr Gly Leu Gly Thr Pro Gln Ser

185 190 195

tat ctt gac cgt tcc gtt gtg ttg aac gtg ggg gag gag atc gac cgc 739

Tyr Leu Asp Arg Ser Val Val Leu Asn Val Gly Glu Glu Ile Asp Arg

200 205 210

gat cgc ttt ttg cgc cta ttg gta gat att caa tac gaa cgc aat gat 787

Asp Arg Phe Leu Arg Leu Leu Val Asp Ile Gln Tyr Glu Arg Asn Asp

215 220 225

gtg ggc ttt act cgt ggt gct ttc cgc gtg aag ggc gat acc gtg gac 835

Val Gly Phe Thr Arg Gly Ala Phe Arg Val Lys Gly Asp Thr Val Asp

230 235 240 245

atc atc ccg gcc tat gag gaa ttg gcg gtg cgc att gag ttt ttc ggt 883

Ile Ile Pro Ala Tyr Glu Glu Leu Ala Val Arg Ile Glu Phe Phe Gly

250 255 260

gat gaa att gat gcg ttg tac tac atc cat ccc ctg act ggt gac acc 931

Asp Glu Ile Asp Ala Leu Tyr Tyr Ile His Pro Leu Thr Gly Asp Thr

265 270 275

atc cgg cag gtg aat gag atc cgt att ttc cca gct acg cac tat gtt 979

Ile Arg Gln Val Asn Glu Ile Arg Ile Phe Pro Ala Thr His Tyr Val

280 285 290

gcg gga cct gag cgg atg gaa aag gca gtc gct gat att aag gcg gag 1027

Ala Gly Pro Glu Arg Met Glu Lys Ala Val Ala Asp Ile Lys Ala Glu

295 300 305

ttg gaa gtg cgc ctg gct gat ttg gag aac cgt ggc aag tta ttg gaa 1075

Leu Glu Val Arg Leu Ala Asp Leu Glu Asn Arg Gly Lys Leu Leu Glu

310 315 320 325

gcg cag cgt ctt agg atg cgt act gaa tat gac tta gaa atg atc gag 1123

Ala Gln Arg Leu Arg Met Arg Thr Glu Tyr Asp Leu Glu Met Ile Glu

330 335 340

cag gtt ggt ttc tgt tcg ggc att gag aac tat tct cgc cac att gat 1171

Gln Val Gly Phe cys Ser Gly Ile Glu Asn Tyr Ser Arg His Ile Asp

345 350 355

gga cgt ggg gag gga acc gca ccg gcc acg ctg att gac tat ttc cca 1219

Gly Arg Gly Glu Gly Thr Ala Pro Ala Thr Leu Ile Asp Tyr Phe Pro

360 365 370

gag gat ttc ctc acc atc atc gat gag tct cac gtg aca gtc ccg cag 1267

Glu Asp Phe Leu Thr Ile Ile Asp Glu Ser His Val Thr Val Pro Gln

375 380 385

atc ggc ggc atg ttt gag ggc gat atg tcc cgt aaa cgt aac ctc gta 1315

Ile Gly Gly Met Phe Glu Gly Asp Met Ser Arg Lys Arg Asn Leu Val

390 395 400 405

gaa ttc ggt ttc cgc ctg cca tcc gcg atg gat aac cgc cca ttg acc 1363

Glu Phe Gly Phe Arg Leu Pro Ser Ala Met Asp Asn Arg Pro Leu Thr

410 415 420

tgg gag gag ttc gat gaa cgc cgt ggc caa acg gtg ttc atg tct gca 1411

Trp Glu Glu Phe Asp Glu Arg Arg Gly Gln Thr Val Phe Met Ser Ala

425 430 435

act cca ggc aag ttt gag atc gct gct gct gat ggt gag ttt gtg gag 1459

Thr Pro Gly Lys Phe Glu Ile Ala Ala Ala Asp Gly Glu Phe Val Glu

440 445 450

cag gtc att cgc cca aca ggt ctg gtg gat cca aag gtc acc gtc aag 1507

Gln Val Ile Arg Pro Thr Gly Leu Val Asp Pro Lys Val Thr Val Lys

455 460 465

cca acg aag ggg cag att gat gat ctg atc cat gaa att cgc caa cgc 1555

Pro Thr Lys Gly Gln Ile Asp Asp Leu Ile His Glu Ile Arg Gln Arg

470 475 480 485

acc gat aaa gat gag cgc gtt ttg gtc acc aca ttg acc aag aaa atg 1603

Thr Asp Lys Asp Glu Arg Val Leu Val Thr Thr Leu Thr Lys Lys Met

490 495 500

gct gag gat ctt act gat tac ctg ctg gaa aac ggc atc cgc gtg cgc 1651

Ala Glu Asp Leu Thr Asp Tyr Leu Leu Glu Asn Gly Ile Arg Val Arg

505 510 515

tac ctg cac tca gat att gat acc ttg cag cgt gtg gaa ttg ctg cgt 1699

Tyr Leu His Ser Asp Ile Asp Thr Leu Gln Arg Val Glu Leu Leu Arg

520 525 530

cag ctt cgc ctg ggc gaa tac gat gtg ttg gta ggt att aac ctg ctg 1747

Gln Leu Arg Leu Gly Glu Tyr Asp Val Leu Val Gly Ile Asn Leu Leu

535 540 545

cgt gag ggc ctt gac ctg cca gaa gtc tct ctg gtt gcg att ctc gac 1795

Arg Glu Gly Leu Asp Leu Pro Glu Val Ser Leu Val Ala Ile Leu Asp

550 555 560 565

gcc gac aag gaa ggc ttc ctg cgc tcc acc acc tca ctg att cag acc 1843

Ala Asp Lys Glu Gly Phe Leu Arg Ser Thr Thr Ser Leu Ile Gln Thr

570 575 580

att ggc cgc gcc gcc cga aat gtg tcc ggc gag gtc atc atg tac gcc 1891

Ile Gly Arg Ala Ala Arg Asn Val Ser Gly Glu Val Ile Met Tyr Ala

585 590 595

gac aag atc act gat tcg atg cag tat gcc atc gag gaa acc gat cga 1939

Asp Lys Ile Thr Asp Ser Met Gln Tyr Ala Ile Glu Glu Thr Asp Arg

600 605 610

cgc cgt gaa aag cag gtc gct tat aac aag gaa cac ggc atc gat ccg 1987

Arg Arg Glu Lys Gln Val Ala Tyr Asn Lys Glu His Gly Ile Asp Pro

615 620 625

cag ccg ctt cga aag aaa atc gcg gac atc ctc gac cag gtc tat gac 2035

Gln Pro Leu Arg Lys Lys Ile Ala Asp Ile Leu Asp Gln Val Tyr Asp

630 635 640 645

aat tcc gct gat gga gca gga cct tct gcc tct ggc gat gcg gca gtc 2083

Asn Ser Ala Asp Gly Ala Gly Pro Ser Ala Ser Gly Asp Ala Ala Val

650 655 660

gtg gct aaa cct gac gtg tct agc atg ccc gcc aaa gaa gtg caa aag 2131

Val Ala Lys Pro Asp Val Ser Ser Met Pro Ala Lys Glu Val Gln Lys

665 670 675

ctt atc gac gac ctc agc gct cag atg gct gcg gcc gcg cgg gag ctc 2179

Leu Ile Asp Asp Leu Ser Ala Gln Met Ala Ala Ala Ala Arg Glu Leu

680 685 690

aag ttc gag ctg gca ggg cgt ctg cga gat gag atc ttc gag ctc aag 2227

Lys Phe Glu Leu Ala Gly Arg Leu Arg Asp Glu Ile Phe Glu Leu Lys

695 700 705

aag gaa ctg aga ggt atc aag gat gcc ggc atc taagtcagct tgctcactta 2280

Lys Glu Leu Arg Gly Ile Lys Asp Ala Gly Ile

710 715 720

aagcttcgaa 2290

<210>78

<211>720

<212>PRT

<213>谷氨酸棒状杆菌

<400>78

Leu Leu Ala Arg Cys Phe Ala Ala Gly Arg Asn Val Ser Thr Leu Trp

1 5 10 15

Arg Ile Leu Glu Cys Met Ala Phe Ala Ala Glu His Pro Val Leu Ser

20 25 30

His Ser Glu His Arg Pro Val Gly Glu Ile Glu Arg Ser Asp Asp Lys

35 40 45

Phe Val Val Val Ser Glu Phe Glu Pro Ala Gly Asp Gln Pro Ala Ala

50 55 60

Ile Lys Glu Leu Asp Glu Arg Leu Asp Arg Gly Glu Arg Asp Val Val

65 70 75 80

Leu Met Gly Ala Thr Gly Thr Gly Lys Ser Ala Thr Ala Ala Trp Leu

85 90 95

Ile Glu Lys Gln Gln Arg Pro Ala Leu Val Met Ala Pro Asn Lys Thr

100 105 110

Leu Ala Ala Gln Leu Ala Asn Glu Leu Arg Gln Leu Leu Pro Asn Asn

115 120 125

Ala Val Glu Tyr Phe Val Ser Tyr Tyr Asp Tyr Tyr Gln Pro Glu Ala

130 135 140

Tyr Ile Ala Gln Thr Asp Thr Tyr Ile Glu Lys Asp Ser Ser Ile Asn

145 150 155 160

Glu Asp Val Glu Arg Leu Arg His Ser Ala Thr Ser Ser Leu Leu Ser

165 170 175

Arg Arg Asp Val Val Val Val Ser Ser Val Ser Cys Ile Tyr Gly Leu

180 185 190

Gly Thr Pro Gln Ser Tyr Leu Asp Arg Ser Val Val Leu Asn Val Gly

195 200 205

Glu Glu Ile Asp Arg Asp Arg Phe Leu Arg Leu Leu Val Asp Ile Gln

210 215 220

Tyr Glu Arg Asn Asp Val Gly Phe Thr Arg Gly Ala Phe Arg Val Lys

225 230 235 240

Gly Asp Thr Val Asp Ile Ile Pro Ala Tyr Glu Glu Leu Ala Val Arg

245 250 255

Ile Glu Phe Phe Gly Asp Glu Ile Asp Ala Leu Tyr Tyr Ile His Pro

260 265 270

Leu Thr Gly Asp Thr Ile Arg Gln Val Asn Glu Ile Arg Ile Phe Pro

275 280 285

Ala Thr His Tyr Val Ala Gly Pro Glu Arg Met Glu Lys Ala Val Ala

290 295 300

Asp Ile Lys Ala Glu Leu Glu Val Arg Leu Ala Asp Leu Glu Asn Arg

305 310 315 320

Gly Lys Leu Leu Glu Ala Gln Arg Leu Arg Met Arg Thr Glu Tyr Asp

325 330 335

Leu Glu Met Ile Glu Gln Val Gly Phe Cys Ser Gly Ile Glu Asn Tyr

340 345 350

Ser Arg His Ile Asp Gly Arg Gly Glu Gly Thr Ala Pro Ala Thr Leu

355 360 365

Ile Asp Tyr Phe Pro Glu Asp Phe Leu Thr Ile Ile Asp Glu Ser His

370 375 380

Val Thr Val Pro Gln Ile Gly Gly Met Phe Glu Gly Asp Met Ser Arg

385 390 395 400

Lys Arg Asn Leu Val Glu Phe Gly Phe Arg Leu Pro Ser Ala Met Asp

405 410 415

Asn Arg Pro Leu Thr Trp Glu Glu Phe Asp Glu Arg Arg Gly Gln Thr

420 425 430

Val Phe Met Ser Ala Thr Pro Gly Lys Phe Glu Ile Ala Ala Ala Asp

435 440 445

Gly Glu Phe Val Glu Gln Val Ile Arg Pro Thr Gly Leu Val Asp Pro

450 455 460

Lys Val Thr Val Lys Pro Thr Lys Gly Gln Ile Asp Asp Leu Ile His

465 470 475 480

Glu Ile Arg Gln Arg Thr Asp Lys Asp Glu Arg Val Leu Val Thr Thr

485 490 495

Leu Thr Lys Lys Met Ala Glu Asp Leu Thr Asp Tyr Leu Leu Glu Asn

500 505 510

Gly Ile Arg Val Arg Tyr Leu His Ser Asp Ile Asp Thr Leu Gln Arg

515 520 525

Val Glu Leu Leu Arg Gln Leu Arg Leu Gly Glu Tyr Asp Val Leu Val

530 535 540

Gly Ile Asn Leu Leu Arg Glu Gly Leu Asp Leu Pro Glu Val Ser Leu

545 550 555 560

Val Ala Ile Leu Asp Ala Asp Lys Glu Gly Phe Leu Arg Ser Thr Thr

565 570 575

Ser Leu Ile Gln Thr Ile Gly Arg Ala Ala Arg Asn Val Ser Gly Glu

580 585 590

Val Ile Met Tyr Ala Asp Lys Ile Thr Asp Ser Met Gln Tyr Ala Ile

595 600 605

Glu Glu Thr Asp Arg Arg Arg Glu Lys Gln Val Ala Tyr Asn Lys Glu

610 615 620

His Gly Ile Asp Pro Gln Pro Leu Arg Lys Lys Ile Ala Asp Ile Leu

625 630 635 640

Asp Gln Val Tyr Asp Asn Ser Ala Asp Gly Ala Gly Pro Ser Ala Ser

645 650 655

Gly Asp Ala Ala Val Val Ala Lys Pro Asp Val Ser Ser Met Pro Ala

660 665 670

Lys Glu Val Gln Lys Leu Ile Asp Asp Leu Ser Ala Gln Met Ala Ala

675 680 685

Ala Ala Arg Glu Leu Lys Phe Glu Leu Ala Gly Arg Leu Arg Asp Glu

690 695 700

Ile Phe Glu Leu Lys Lys Glu Leu Arg Gly Ile Lys Asp Ala Gly Ile

705 710 715 720

<210>79

<211>1087

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1057)

<223>RXA02736

<400>79

cagaggatta cccagcgggt acgtggggtc caaagagcgc tgatgaaatg ctttcccgca 60

acggtcacac ctggcgcagg ccataattta ggggcaaaaa atg atc ttt gaa ctt 115

Met Ile Phe Glu Leu

1 5

ccg gat acc acc acc cag caa att tcc aag acc cta act cga ctg cgt 163

Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr Leu Thr Arg Leu Arg

10 15 20

gaa tcg ggc acc cag gtc acc acc ggc cga gtg ctc acc ctc atc gtg 211

Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val Leu Thr Leu Ile Val

25 30 35

gtc act gac tcc gaa agc gat gtc gct gca gtt acc gag tcc acc aat 259

Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val Thr Glu Ser Thr Asn

40 45 50

gaa gcc tcg cgc gag cac cca tct cgc gtg atc att ttg gtg gtt ggc 307

Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile Ile Leu Val Val Gly

55 60 65

gat aaa act gca gaa aac aaa gtt gac gca gaa gtc cgt atc ggt ggc 355

Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu Val Arg Ile Gly Gly

70 75 80 85

gac gct ggt gct tcc gag atg atc atc atg cat ctc aac gga cct gtc 403

Asp Ala Gly Ala Ser Glu Met Ile Ile Met His Leu Asn Gly Pro Val

90 95 100

gct gac aag ctc cag tat gtc gtc aca cca ctg ttg ctt cct gac acc 451

Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu Leu Leu Pro Asp Thr

105 110 115

ccc atc gtt gct tgg tgg cca ggt gaa tca cca aag aat cct tcc cag 499

Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro Lys Asn Pro Ser Gln

120 125 130

gac cca att gga cgc atc gca caa cga cgc atc act gat gct ttg tac 547

Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile Thr Asp Ala Leu Tyr

135 140 145

gac cgt gat gac gca cta gaa gat cgt gtt gag aac tat cac cca ggt 595

Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu Asn Tyr His Pro Gly

150 155 160 165

gat acc gac atg acg tgg gcg cgc ctt acc cag tgg cgg gga ctt gtt 643

Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln Trp Arg Gly Leu Val

170 175 180

gcc tcc tca ttg gat cac cca cca cac agc gaa atc act tcc gtg agg 691

Ala Ser Ser Leu Agp Hig Pro Pro His Ser Glu Ile Thr Ser Val Arg

185 190 195

ctg acc ggt gca agc ggc agt acc tcg gtg gat ttg gct gca ggc tgg 739

Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp Leu Ala Ala Gly Trp

200 205 210

ttg gcg cgg agg ctg aaa gtg cct gtg atc cgc gag gtg aca gat gct 787

Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg Glu Val Thr Asp Ala

215 220 225

ccc acc gtg cca acc gat gag ttt ggt act cca ctg ctg gct atc cag 835

Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro Leu Leu Ala Ile Gln

230 235 240 245

cgc ctg gag atc gtt cgc acc acc ggc tcg atc atc atc acc atc tat 883

Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile Ile Ile Thr Ile Tyr

250 255 260

gac gct cat acc ctt cag gta gag atg ccg gaa tcc ggc aat gcc cca 931

Asp Ala His Thr Leu Gln Val Glu Met Pro Glu Ser Gly Asn Ala Pro

265 270 275

tcg ctg gtg gct att ggt cgt cga agt gag tcc gac tgc ttg tct gag 979

Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser Asp Cys Leu Ser Glu

280 285 290

gag ctt cgc cac atg gat cca gat ttg ggc tac cag cac gca cta tcc 1027

Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr Gln His Ala Leu Ser

295 300 305

ggc ttg tcc agc gtc aag ctg gaa acc gtc taaggagaaa tacaacacta 1077

Gly Leu Ser Ser Val Lys Leu Glu Thr Val

310 315

tggttgatgt 1087

<210>80

<211>319

<212>PRT

<213>谷氨酸棒状杆菌

<400>80

Met Ile Phe Glu Leu Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr

1 5 10 15

Leu Thr Arg Leu Arg Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val

20 25 30

Leu Thr Leu Ile Val Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val

35 40 45

Thr Glu Ser Thr Asn Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile

50 55 60

Ile Leu Val Val Gly Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu

65 70 75 80

Val Arg Ile Gly Gly Asp Ala Gly Ala Ser Glu Met Ile Ile Met His

85 90 95

Leu Asn Gly Pro Val Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu

100 105 110

Leu Leu Pro Asp Thr Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro

115 120 125

Lys Asn Pro Ser Gln Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile

130 135 140

Thr Asp Ala Leu Tyr Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu

145 150 155 160

Asn Tyr His Pro Gly Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln

165 170 175

Trp Arg Gly Leu Val Ala Ser Ser Leu Asp His Pro Pro His Ser Glu

180 185 190

Ile Thr Ser Val Arg Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp

195 200 205

Leu Ala Ala Gly Trp Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg

210 215 220

Glu Val Thr Asp Ala Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro

225 230 235 240

Leu Leu Ala Ile Gln Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile

245 250 255

Ile Ile Thr Ile Tyr Asp Ala His Thr Leu Gln Val Glu Met Pro Glu

260 265 270

Ser Gly Asn Ala Pro Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser

275 280 285

Asp Cys Leu Ser Glu Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr

290 295 300

Gln His Ala Leu Ser Gly Leu Ser Ser Val Lys Leu Glu Thr Val

305 310 315

<210>81

<211>2479

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2449)

<223>RXA02742

<400>81

gtatcggtaa aaggcccaat caaggattct tggttgggtc tttttccatg cttttcatgc 60

ttcgaacagt ccagaatagc cctgacctag acttagagct atg tcc gaa caa aga 115

Met Ser Glu Gln Arg

1 5

ctc gat cag ctt gag cga cgg ctt tct gaa ctg gaa cgg gag atc gcc 163

Leu Asp Gln Leu Glu Arg Arg Leu Ser Glu Leu Glu Arg Glu Ile Ala

10 15 20

gcg att cgt cag gag atc cgc cag gaa cgc cta gtg ctt ccg gaa ccg 211

Ala Ile Arg Gln Glu Ile Arg Gln Glu Arg Leu Val Leu Pro Glu Pro

25 30 35

gaa cct gtg aaa gtt gat aca gtc atc gcc acc gaa gcg acc gga gtc 259

Glu Pro Val Lys Val Asp Thr Val Ile Ala Thr Glu Ala Thr Gly Val

40 45 50

aat gca tcg tcg ggt ccg gag gcg aag atc gct ttg ttc atg gag agg 307

Asn Ala Ser Ser Gly Pro Glu Ala Lys Ile Ala Leu Phe Met Glu Arg

55 60 65

ttt agt ggt cgc cac gat gtg tat gcg cgg cgc tgg acc agc aga aaa 355

Phe Ser Gly Arg His Asp Val Tyr Ala Arg Arg Trp Thr Ser Arg Lys

70 75 80 85

acg ggc aaa agt gga tgg tcg ccg gct act cgc cag ggt ttt tac tca 403

Thr Gly Lys Ser Gly Trp Ser Pro Ala Thr Arg Gln Gly Phe Tyr Ser

90 95 100

aaa gac acc aca ccg aag gac tat ctc ccc ttc acc gtt gac acc gtc 451

Lys Asp Thr Thr Pro Lys Asp Tyr Leu Pro Phe Thr Val Asp Thr Val

105 110 115

aat gcg cat ctg cgc cgg ggc ggc gac cat atc ggt ctc tat gtg atg 499

Asn Ala His Leu Arg Arg Gly Gly Asp His Ile Gly Leu Tyr Val Met

120 125 130

gtc ccc atc gac acg tgc aaa ctt ctc gcc tgc gat ttc gac gat ggc 547

Val Pro Ile Asp Thr Cys Lys Leu Leu Ala cys Asp Phe Asp Asp Gly

135 140 145

acc tgg aag caa gat gcg gcc gct ttc gtg tca gcc tgc acc gac cac 595

Thr Trp Lys Gln Asp Ala Ala Ala Phe Val Ser Ala Cys Thr Asp His

150 155 160 165

gga atc gat gcg ttg gct gaa att tct cga tcc gac gac ggc gcc ccc 643

Gly Ile Asp Ala Leu Ala Glu Ile Ser Arg Ser Asp Asp Gly Ala Pro

170 175 180

gtg tgg ata ttt ttc gat acc cca atc tcc gcg atg ctg gct cgg cgc 691

Val Trp Ile Phe Phe Asp Thr Pro Ile Ser Ala Met Leu Ala Arg Arg

185 190 195

cta ggt ttt gcc atg ctc cgc caa gcc atg aac tcc cgc cct gac atg 739

Leu Gly Phe Ala Met Leu Arg Gln Ala Met Asn Ser Arg Pro Asp Met

200 205 210

gat atg tct tct tat gat cgc ttc ttc cct gct caa gac acc atc gca 787

Asp Met Ser Ser Tyr Asp Arg Phe Phe Pro Ala Gln Asp Thr Ile Ala

215 220 225

acg cgc gca aac gga agc gca cgg ctg gga aat ttg atc gcg ctg ccc 835

Thr Arg Ala Asn Gly Ser Ala Arg Leu Gly Asn Leu Ile Ala Leu Pro

230 235 240 245

ctc aac ggc gac tgt cga gcc cgc aac acc gcc gtc ttc gcc gat tcg 883

Leu Asn Gly Asp Cys Arg Ala Arg Asn Thr Ala Val Phe Ala Asp Ser

250 255 260

gaa acg tgg gtt ccc ttc gaa gat cct ttc gca gcg ctc gcg gcc atc 931

Glu Thr Trp Val Pro Phe Glu Asp Pro Phe Ala Ala Leu Ala Ala Ile

265 270 275

acg cca cta gcc acc gaa aaa atc gag cag atc ctt gcc acc acg cag 979

Thr Pro Leu Ala Thr Glu Lys Ile Glu Gln Ile Leu Ala Thr Thr Gln

280 285 290

gaa aaa ttt ggc ccc gaa ccc gaa cac atc aaa cgc ccc acc cgc gcc 1027

Glu Lys Phe Gly Pro Glu Pro Glu His Ile Lys Arg Pro Thr Arg Ala

295 300 305

gaa ctc aaa cag gtt aaa gcc aac ggc gaa acc atc aaa ctc acc atc 1075

Glu Leu Lys Gln Val Lys Ala Asn Gly Glu Thr Ile Lys Leu Thr Ile

310 315 320 325

acc aac gag ctg agc gtc ccc acc gaa agg tta ccc gcg gcc gtc atc 1123

Thr Asn Glu Leu Ser Val Pro Thr Glu Arg Leu Pro Ala Ala Val Ile

330 335 340

gcg gag att aaa cac cgg gcg gta atc cca aac cct gag ttt tat cgt 1171

Ala Glu Ile Lys His Arg Ala Val Ile Pro Asn Pro Glu Phe Tyr Arg

345 350 355

cga caa gcg caa aga ttt tcg acc ttc ggc gtg ccg cgc atc gtc atc 1219

Arg Gln Ala Gln Arg Phe Ser Thr Phe Gly Val Pro Arg Ile Val Ile

360 365 370

cgc ttc gcc cag gcc gag cag cgc ttg ctg ctc cca cgc ggg ctt gtc 1267

Arg Phe Ala Gln Ala Glu Gln Arg Leu Leu Leu Pro Arg Gly Leu Val

375 380 385

gac gac acc ctc cgg atc ctc acc ctc gcc ggg tac aaa gtc agc gtc 1315

Asp Asp Thr Leu Arg Ile Leu Thr Leu Ala Gly Tyr Lys Val Ser Val

390 395 400 405

atc tgg cct cgg caa act cgg aaa acc atc gac gcg tct ttc gag ggc 1363

Ile Trp Pro Arg Gln Thr Arg Lys Thr Ile Asp Ala Ser Phe Glu Gly

410 415 420

gaa ttg cga tcc atg caa caa gag gga atc gac tcg ctc aaa ggc caa 1411

Glu Leu Arg Ser Met Gln Gln Glu Gly Ile Asp Ser Leu Lys Gly Gln

425 430 435

cgc acc ggc gta ttg gta gca ccg ccg ggc gct gga aaa aca gtg atg 1459

Arg Thr Gly Val Leu Val Ala Pro Pro Gly Ala Gly Lys Thr Val Met

440 445 450

gcc tgt gca ctc atc gcg aac aga aaa atc ccc acc gca gtg ata gtc 1507

Ala Cys Ala Leu Ile Ala Asn Arg Lys Ile Pro Thr Ala Val Ile Val

455 460 465

aac cgt gca gaa ttg att tcc caa tgg cgg gat cgt ctc gcg caa tac 1555

Asn Arg Ala Glu Leu Ile Ser Gln Trp Arg Asp Arg Leu Ala Gln Tyr

470 475 480 485

ctg agc atc gac gca gac tcc atc gga cag atc ggc gcg ggc cga cgc 1603

Leu Ser Ile Asp Ala Asp Ser Ile Gly Gln Ile Gly Ala Gly Arg Arg

490 495 500

aaa acc acc gga att atc gat ctc atc acc gtc caa tcc ttg agc cgt 1651

Lys Thr Thr Gly Ile Ile Asp Leu Ile Thr Val Gln Ser Leu Ser Arg

505 510 515

aaa gat tcc gat ccg aaa att ttg gaa caa tac ggc caa atc atc gtc 1699

Lys Asp Ser Asp Pro Lys Ile Leu Glu Gln Tyr Gly Gln Ile Ile Val

520 525 530

gac gag tgc cac aac atc gca gcc cca ggc gcc gaa gcc gca ttg aac 1747

Asp Glu Cys His Asn Ile Ala Ala Pro Gly Ala Glu Ala Ala Leu Asn

535 540 545

cag gtc aag gcc ccc tac tgg ctg ggt cta acc gcc acg ccg ttt cgt 1795

Gln Val Lys Ala Pro Tyr Trp Leu Gly Leu Thr Ala Thr Pro Phe Arg

550 555 560 565

tca gac cac atg gat gaa atc atc acc atg cag tgc ggt cct gtg cgc 1843

Ser Asp His Met Asp Glu Ile Ile Thr Met Gln Cys Gly Pro Val Arg

570 575 580

cac cgc atg gaa gtg gca aca gac aat gaa cag cgc ttg att cac atc 1891

His Arg Met Glu Val Ala Thr Asp Asn Glu Gln Arg Leu Ile His Ile

585 590 595

cac gaa acc tct ttc gac tct gag gaa acc acc gaa atc cag gat ctc 1939

His Glu Thr Ser Phe Asp Ser Glu Glu Thr Thr Glu Ile Gln Asp Leu

600 605 610

tac aat gag ctc gcg gtc gat tct gcc cga aat gcg caa atc act gcc 1987

Tyr Asn Glu Leu Ala Val Asp Ser Ala Arg Asn Ala Gln Ile Thr Ala

615 620 625

gaa gtg cac aaa gcg ctt gaa gct ggc gac cga tgt cta gtt ttg gtc 2035

Glu Val His Lys Ala Leu Glu Ala Gly Asp Arg Cys Leu Val Leu Val

630 635 640 645

aac cga att gca gcc ctt gaa gca ctg acc agc agt att acc gaa tct 2083

Asn Arg Ile Ala Ala Leu Glu Ala Leu Thr Ser Ser Ile Thr Glu Ser

650 655 660

ggc gat cac act gtc tta gtg atg cat ggc cgc caa acc caa gag gag 2131

Gly Asp His Thr Val Leu Val Met His Gly Arg Gln Thr Gln Glu Glu

665 670 675

cga gtt cac ctt cgt gcg caa ctt gcc tca ttg agt gaa aag cag gat 2179

Arg Val His Leu Arg Ala Gln Leu Ala Ser Leu Ser Glu Lys Gln Asp

680 685 690

ccg ttt gta ctg gtc gcg atg aat aaa gtc gcc ggc gaa ggc ctt gac 2227

Pro Phe Val Leu Val Ala Met Asn Lys Val Ala Gly Glu Gly Leu Asp

695 700 705

atc ccc agc ctc aac acg ctg ttt ttg gca gcg ccg gtg tcc ttc aag 2275

Ile Pro Ser Leu Asn Thr Leu Phe Leu Ala Ala Pro Val Ser Phe Lys

710 715 720 725

ggg ctg gtg att cag caa atc ggc cga gtt act cgc gca acc ggt gat 2323

Gly Leu Val Ile Gln Gln Ile Gly Arg Val Thr Arg Ala Thr Gly Asp

730 735 740

caa aac gct cct ccg gtg act gcc acg gtc cat gat ttt gtt gat tcc 2371

Gln Asn Ala Pro Pro Val Thr Ala Thr Val His Asp Phe Val Asp Ser

745 750 755

aag att ccg aca ctc aaa cgc atg cac ggt cgc cga ttg cgg gct atg 2419

Lys Ile Pro Thr Leu Lys Arg Met His Gly Arg Arg Leu Arg Ala Met

760 765 770

caa aag gaa gga ttc gct gtt tcg gag cct tgaggaggac cagaccaaac 2469

Gln Lys Glu Gly Phe Ala Val Ser Glu Pro

775 780

cagcgtgccc 2479

<210>82

<211>783

<212>PRT

<213>谷氨酸棒状杆菌

<400>82

Met Ser Glu Gln Arg Leu Asp Gln Leu Glu Arg Arg Leu Ser Glu Leu

1 5 10 15

Glu Arg Glu Ile Ala Ala Ile Arg Gln Glu Ile Arg Gln Glu Arg Leu

20 25 30

Val Leu Pro Glu Pro Glu Pro Val Lys Val Asp Thr Val Ile Ala Thr

35 40 45

Glu Ala Thr Gly Val Asn Ala Ser Ser Gly Pro Glu Ala Lys Ile Ala

50 55 60

Leu Phe Met Glu Arg Phe Ser Gly Arg His Asp Val Tyr Ala Arg Arg

65 70 75 80

Trp Thr Ser Arg Lys Thr Gly Lys Ser Gly Trp Ser Pro Ala Thr Arg

85 90 95

Gln Gly Phe Tyr Ser Lys Asp Thr Thr Pro Lys Asp Tyr Leu Pro Phe

100 105 110

Thr Val Asp Thr Val Asn Ala His Leu Arg Arg Gly Gly Asp His Ile

115 120 125

Gly Leu Tyr Val Met Val Pro Ile Asp Thr Cys Lys Leu Leu Ala Cys

130 135 140

Asp Phe Asp Asp Gly Thr Trp Lys Gln Asp Ala Ala Ala Phe Val Ser

145 150 155 160

Ala Cys Thr Asp His Gly Ile Asp Ala Leu Ala Glu Ile Ser Arg Ser

165 170 175

Asp Asp Gly Ala Pro Val Trp Ile Phe Phe Asp Thr Pro Ile Ser Ala

180 185 190

Met Leu Ala Arg Arg Leu Gly Phe Ala Met Leu Arg Gln Ala Met Asn

195 200 205

Ser Arg Pro Asp Met Asp Met Ser Ser Tyr Asp Arg Phe Phe Pro Ala

210 215 220

Gln Asp Thr Ile Ala Thr Arg Ala Asn Gly Ser Ala Arg Leu Gly Asn

225 230 235 240

Leu Ile Ala Leu Pro Leu Asn Gly Asp Cys Arg Ala Arg Asn Thr Ala

245 250 255

Val Phe Ala Asp Ser Glu Thr Trp Val Pro Phe Glu Asp Pro Phe Ala

260 265 270

Ala Leu Ala Ala Ile Thr Pro Leu Ala Thr Glu Lys Ile Glu Gln Ile

275 280 285

Leu Ala Thr Thr Gln Glu Lys Phe Gly Pro Glu Pro Glu His Ile Lys

290 295 300

Arg Pro Thr Arg Ala Glu Leu Lys Gln Val Lys Ala Asn Gly Glu Thr

305 310 315 320

Ile Lys Leu Thr Ile Thr Asn Glu Leu Ser Val Pro Thr Glu Arg Leu

325 330 335

Pro Ala Ala Val Ile Ala Glu Ile Lys His Arg Ala Val Ile Pro Asn

340 345 350

Pro Glu Phe Tyr Arg Arg Gln Ala Gln Arg Phe Ser Thr Phe Gly Val

355 360 365

Pro Arg Ile Val Ile Arg Phe Ala Gln Ala Glu Gln Arg Leu Leu Leu

370 375 380

Pro Arg Gly Leu Val Asp Asp Thr Leu Arg Ile Leu Thr Leu Ala Gly

385 390 395 400

Tyr Lys Val Ser Val Ile Trp Pro Arg Gln Thr Arg Lys Thr Ile Asp

405 410 415

Ala Ser Phe Glu Gly Glu Leu Arg Ser Met Gln Gln Glu Gly Ile Asp

420 425 430

Ser Leu Lys Gly Gln Arg Thr Gly Val Leu Val Ala Pro Pro Gly Ala

435 440 445

Gly Lys Thr Val Met Ala Cys Ala Leu Ile Ala Asn Arg Lys Ile Pro

450 455 460

Thr Ala Val Ile Val Asn Arg Ala Glu Leu Ile Ser Gln Trp Arg Asp

465 470 475 480

Arg Leu Ala Gln Tyr Leu Ser Ile Asp Ala Asp Ser Ile Gly Gln Ile

485 490 495

Gly Ala Gly Arg Arg Lys Thr Thr Gly Ile Ile Asp Leu Ile Thr Val

500 505 510

Gln Ser Leu Ser Arg Lys Asp Ser Asp Pro Lys Ile Leu Glu Gln Tyr

515 520 525

Gly Gln Ile Ile Val Asp Glu Cys His Asn Ile Ala Ala Pro Gly Ala

530 535 540

Glu Ala Ala Leu Asn Gln Val Lys Ala Pro Tyr Trp Leu Gly Leu Thr

545 550 555 560

Ala Thr Pro Phe Arg Ser Asp His Met Asp Glu Ile Ile Thr Met Gln

565 570 575

Cys Gly Pro Val Arg His Arg Met Glu Val Ala Thr Asp Asn Glu Gln

580 585 590

Arg Leu Ile His Ile His Glu Thr Ser Phe Asp Ser Glu Glu Thr Thr

595 600 605

Glu Ile Gln Asp Leu Tyr Asn Glu Leu Ala Val Asp Ser Ala Arg Asn

610 615 620

Ala Gln Ile Thr Ala Glu Val His Lys Ala Leu Glu Ala Gly Asp Arg

625 630 635 640

Cys Leu Val Leu Val Asn Arg Ile Ala Ala Leu Glu Ala Leu Thr Ser

645 650 655

Ser Ile Thr Glu Ser Gly Asp His Thr Val Leu Val Met His Gly Arg

660 665 670

Gln Thr Gln Glu Glu Arg Val His Leu Arg Ala Gln Leu Ala Ser Leu

675 680 685

Ser Glu Lys Gln Asp Pro Phe Val Leu Val Ala Met Asn Lys Val Ala

690 695 700

Gly Glu Gly Leu Asp Ile Pro Ser Leu Asn Thr Leu Phe Leu Ala Ala

705 710 715 720

Pro Val Ser Phe Lys Gly Leu Val Ile Gln Gln Ile Gly Arg Val Thr

725 730 735

Arg Ala Thr Gly Asp Gln Asn Ala Pro Pro Val Thr Ala Thr Val His

740 745 750

Asp Phe Val Asp Ser Lys Ile Pro Thr Leu Lys Arg Met His Gly Arg

755 760 765

Arg Leu Arg Ala Met Gln Lys Glu Gly Phe Ala Val Ser Glu Pro

770 775 780

<210>83

<211>1771

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1741)

<223>RXA02748

<400>83

caggtcgttg gcaggtagct gacgtgacct gagcgggggc aaccggctaa catgtaggga 60

ttagcaaccc cgcactctag aacctactgg gagttcattc gtg ttt gag tca ctg 115

Val Phe Glu Ser Leu

1 5

tcc gat cgg ttg aat agc gcg ctt tcc ggc ctg cgc ggc aaa gga aag 163

Ser Asp Arg Leu Asn Ser Ala Leu Ser Gly Leu Arg Gly Lys Gly Lys

10 15 20

ctc acc gag gca gac atc aat gca acc aca cgc gag atc cgt ctc gcg 211

Leu Thr Glu Ala Asp Ile Asn Ala Thr Thr Arg Glu Ile Arg Leu Ala

25 30 35

ctg ctg gaa gct gac gtt tca tta acg gtt gtt cgt gcc ttc att aac 259

Leu Leu Glu Ala Asp Val Ser Leu Thr Val Val Arg Ala Phe Ile Asn

40 45 50

cga atc aag gaa cgc gcc gct ggt gca gaa gtt tct cag gca ctc aac 307

Arg Ile Lys Glu Arg Ala Ala Gly Ala Glu Val Ser Gln Ala Leu Asn

55 60 65

ccc gcg cag caa gtc atc aag atc gtc aac gag gaa ctg gtt cag atc 355

Pro Ala Gln Gln Val Ile Lys Ile Val Asn Glu Glu Leu Val Gln Ile

70 75 80 85

ctc ggt ggc gaa acc cgc cga ctg tca ctg gcc aaa aac cca ccg acc 403

Leu Gly Gly Glu Thr Arg Arg Leu Ser Leu Ala Lys Asn Pro Pro Thr

90 95 100

gtc atc atg ctg gca ggt ctg cag ggt gca ggt aag acc acc ctc gca 451

Val Ile Met Leu Ala Gly Leu Gln Gly Ala Gly Lys Thr Thr Leu Ala

105 110 115

ggt aaa ctg tcc aag cac ctg gtc aag cag ggt cac act cct atg ctt 499

Gly Lys Leu Ser Lys His Leu Val Lys Gln Gly His Thr Pro Met Leu

120 125 130

gtt gcc tgt gac ctt cag cgt cca ggc gca gtt cag cag ctg caa att 547

Val Ala Cys Asp Leu Gln Arg Pro Gly Ala Val Gln Gln Leu Gln Ile

135 140 145

gtg ggt gaa cgc gca ggc gtt acc act ttc gca ccg gat cca ggc acc 595

Val Gly Glu Arg Ala Gly Val Thr Thr Phe Ala Pro Asp Pro Gly Thr

150 155 160 165

agc atc gac tcc ctc gag cac gaa atg ggc acc tcc cac ggt gat cca 643

Ser Ile Asp Ser Leu Glu His Glu Met Gly Thr Ser His Gly Asp Pro

170 175 180

gtc gag gta gcg cgc gca ggt atc gaa gaa gcc aag cgc acc cag cac 691

Val Glu Val Ala Arg Ala Gly Ile Glu Glu Ala Lys Arg Thr Gln His

185 190 195

gac atc gtg atc gtg gat acc gca ggt cgc ctc ggt atc gat gaa acc 739

Asp Ile Val Ile Val Asp Thr Ala Gly Arg Leu Gly Ile Asp Glu Thr

200 205 210

ctg atg act cag gca cgc aac atc cgc gaa gcc atc aac cct gat gaa 787

Leu Met Thr Gln Ala Arg Asn Ile Arg Glu Ala Ile Asn Pro Asp Glu

215 220 225

gtg ctc ttt gtc att gac tcc atg att ggt caa gac gcc gta gac acc 835

Val Leu Phe Val Ile Asp Ser Met Ile Gly Gln Asp Ala Val Asp Thr

230 235 240 245

gcc gaa gca ttc cgc gac ggc gtc gac ttc acc ggt gtt gtc ctg acc 883

Ala Glu Ala Phe Arg Asp Gly Val Asp Phe Thr Gly Val Val Leu Thr

250 255 260

aag ctt gat ggc gac gcc cgc ggt ggt gct gca cta tcc atc cgt gaa 931

Lys Leu Asp Gly Asp Ala Arg Gly Gly Ala Ala Leu Ser Ile Arg Glu

265 270 275

gtc acc ggc aag ccc atc atg ttt gcc tcc act ggt gaa aaa ctc gac 979

Val Thr Gly Lys Pro Ile Met Phe Ala Ser Thr Gly Glu Lys Leu Asp

280 285 290

gac ttc gac gtc ttc cac cca gag cgc atg gcc agc cga atc ctg ggc 1027

Asp Phe Asp Val Phe His Pro Glu Arg Met Ala Ser Arg Ile Leu Gly

295 300 305

atg ggt gac gta ctg tca ctc atc gag cag gcc gaa gca gtc atg gat 1075

Met Gly Asp Val Leu Ser Leu Ile Glu Gln Ala Glu Ala Val Met Asp

310 315 320 325

cag gaa aag gca gag gtc gct gcc cag aag ttg ggc tcc ggc gag ctc 1123

Gln Glu Lys Ala Glu Val Ala Ala Gln Lys Leu Gly Ser Gly Glu Leu

330 335 340

acc ctg gaa gac ttc ctt gac caa atg ctg atg atc cgc cgc atg gga 1171

Thr Leu Glu Asp Phe Leu Asp Gln Met Leu Met Ile Arg Arg Met Gly

345 350 355

cca atc ggc aac atc ctc aag atg ctg cct ggt ggc aag cag atg tcc 1219

Pro Ile Gly Asn Ile Leu Lys Met Leu Pro Gly Gly Lys Gln Met Ser

360 365 370

caa atg gcg gac atg gtt gat gag aag caa ctc gac cgc atc cag gcg 1267

Gln Met Ala Asp Met Val Asp Glu Lys Gln Leu Asp Arg Ile Gln Ala

375 380 385

att atc cgc ggt atg acc ccg gcc gag cgc gat aat cca aag atc ctc 1315

Ile Ile Arg Gly Met Thr Pro Ala Glu Arg Asp Asn Pro Lys Ile Leu

390 395 400 405

aac gct tcc agg cgc aag cgc atc gcc aac ggt tcc ggt gtg acc gtg 1363

Asn Ala Ser Arg Arg Lys Arg Ile Ala Asn Gly Ser Gly Val Thr Val

410 415 420

tcc gaa gta aac aaa ctt gtt gaa cgc ttc ttc gag gct cgc aag atg 1411

Ser Glu Val Asn Lys Leu Val Glu Arg Phe Phe Glu Ala Arg Lys Met

425 430 435

atg ggt caa atg gct ggc cag ttt ggc atg ggt cct gga tcc cgc agt 1459

Met Gly Gln Met Ala Gly Gln Phe Gly Met Gly Pro Gly Ser Arg Ser

440 445 450

gca acc aag aag caa gcc aag ggc cgc aag ggt aag aac ggc aag cgt 1507

Ala Thr Lys Lys Gln Ala Lys Gly Arg Lys Gly Lys Asn Gly Lys Arg

455 460 465

aaa cca gcc aag aag ggc cca acc cag cca aag atg cca atg ggc ggt 1555

Lys Pro Ala Lys Lys Gly Pro Thr Gln Pro Lys Met Pro Met Gly Gly

470 475 480 485

atg cca gga atg cct ggg atg ccg ggt atg ggt gga gcc gga atg cct 1603

Met Pro Gly Met Pro Gly Met Pro Gly Met Gly Gly Ala Gly Met Pro

490 495 500

gac ctt gct gaa cta cag aag cag ctt ggt gga gca ggt ggc ggt atg 1651

Asp Leu Ala Glu Leu Gln Lys Gln Leu Gly Gly Ala Gly Gly Gly Met

505 510 515

gga ggc ctt ggt ggc gga ctc ccg ggc atg cca aag ccg cct aaa ggc 1699

Gly Gly Leu Gly Gly Gly Leu Pro Gly Met Pro Lys Pro Pro Lys Gly

520 525 530

atg gag aac ata gat ctc aac aac cta gac ttc ggt aag aag 1741

Met Glu Asn Ile Asp Leu Asn Asn Leu Asp Phe Gly Lys Lys

535 540 545

taactttgct ttagttggtc ggcgcatcac 1771

<210>84

<211>547

<212>PRT

<213>谷氨酸棒状杆菌

<400>84

Val Phe Glu Ser Leu Ser Asp Arg Leu Asn Ser Ala Leu Ser Gly Leu

1 5 10 15

Arg Gly Lys Gly Lys Leu Thr Glu Ala Asp Ile Asn Ala Thr Thr Arg

20 25 30

Glu Ile Arg Leu Ala Leu Leu Glu Ala Asp Val Ser Leu Thr Val Val

35 40 45

Arg Ala Phe Ile Asn Arg Ile Lys Glu Arg Ala Ala Gly Ala Glu Val

50 55 60

Ser Gln Ala Leu Asn Pro Ala Gln Gln Val Ile Lys Ile Val Asn Glu

65 70 75 80

Glu Leu Val Gln Ile Leu Gly Gly Glu Thr Arg Arg Leu Ser Leu Ala

85 90 95

Lys Asn Pro Pro Thr Val Ile Met Leu Ala Gly Leu Gln Gly Ala Gly

100 105 110

Lys Thr Thr Leu Ala Gly Lys Leu Ser Lys His Leu Val Lys Gln Gly

115 120 125

His Thr Pro Met Leu Val Ala Cys Asp Leu Gln Arg Pro Gly Ala Val

130 135 140

Gln Gln Leu Gln Ile Val aly Glu Arg Ala Gly Val Thr Thr Phe Ala

145 150 155 160

Pro Asp Pro Gly Thr Ser Ile Asp Ser Leu Glu His Glu Met Gly Thr

165 170 175

Ser His Gly Asp Pro Val Glu Val Ala Arg Ala Gly Ile Glu Glu Ala

180 185 190

Lys Arg Thr Gln His Asp Ile Val Ile Val Asp Thr Ala Gly Arg Leu

195 200 205

Gly Ile Asp Glu Thr Leu Met Thr Gln Ala Arg Asn Ile Arg Glu Ala

210 215 220

Ile Asn Pro Asp Glu Val Leu Phe Val Ile Asp Ser Met Ile Gly Gln

225 230 235 240

Asp Ala Val Asp Thr Ala Glu Ala Phe Arg Asp Gly Val Asp Phe Thr

245 250 255

Gly Val Val Leu Thr Lys Leu Asp Gly Asp Ala Arg Gly Gly Ala Ala

260 265 270

Leu Ser Ile Arg Glu Val Thr Gly Lys Pro Ile Met Phe Ala Ser Thr

275 280 285

Gly Glu Lys Leu Asp Asp Phe Asp Val Phe His Pro Glu Arg Met Ala

290 295 300

Ser Arg Ile Leu Gly Met Gly Asp Val Leu Ser Leu Ile Glu Gln Ala

305 310 315 320

Glu Ala Val Met Asp Gln Glu Lys Ala Glu Val Ala Ala Gln Lys Leu

325 330 335

Gly Ser Gly Glu Leu Thr Leu Glu Asp Phe Leu Asp Gln Met Leu Met

340 345 350

Ile Arg Arg Met Gly Pro Ile Gly Asn Ile Leu Lys Met Leu Pro Gly

355 360 365

Gly Lys Gln Met Ser Gln Met Ala Asp Met Val Asp Glu Lys Gln Leu

370 375 380

Asp Arg Ile Gln Ala Ile Ile Arg Gly Met Thr Pro Ala Glu Arg Asp

385 390 395 400

Asn Pro Lys Ile Leu Asn Ala Ser Arg Arg Lys Arg Ile Ala Asn Gly

405 410 415

Ser Gly Val Thr Val Ser Glu Val Asn Lys Leu Val Glu Arg Phe Phe

420 425 430

Glu Ala Arg Lys Met Met Gly Gln Met Ala Gly Gln Phe Gly Met Gly

435 440 445

Pro Gly Ser Arg Ser Ala Thr Lys Lys Gln Ala Lys Gly Arg Lys Gly

450 455 460

Lys Asn Gly Lys Arg Lys Pro Ala Lys Lys Gly Pro Thr Gln Pro Lys

465 470 475 480

Met Pro Met Gly Gly Met Pro Gly Met Pro Gly Met Pro Gly Met Gly

485 490 495

Gly Ala Gly Met Pro Asp Leu Ala Glu Leu Gln Lys Gln Leu Gly Gly

500 505 510

Ala Gly Gly Gly Met Gly Gly Leu Gly Gly Gly Leu Pro Gly Met Pro

515 520 525

Lys Pro Pro Lys Gly Met Glu Asn Ile Asp Leu Asn Asn Leu Asp Phe

530 535 540

Gly Lys Lys

545

<210>85

<211>958

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(928)

<223>RXA03070

<400>85

gtggataaaa gggaaaacat aggggtcatg aaatagaaca agcacgaggc ctggtaaata 60

cgaattcgac caagaaaacg taaacacccc aggagtactc gtg cct gcc ctt cca 115

Val Pro Ala Leu Pro

1 5

tca tct atc atc gac ccc ctc tgg cgc cag ttc tcc gcc tta atc cca 163

Ser Ser Ile Ile Asp Pro Leu Trp Arg Gln Phe Ser Ala Leu Ile Pro

10 15 20

ccg gtt atc atc acc cac cca cta ggg tgc cac cgt gca cgc att gct 211

Pro Val Ile Ile Thr His Pro Leu Gly Cys His Arg Ala Arg Ile Ala

25 30 35

gac cgg atc atc gtc gac aaa ctc atc gca gtg ctt gtc ctc ggt gtc 259

Asp Arg Ile Ile Val Asp Lys Leu Ile Ala Val Leu Val Leu Gly Val

40 45 50

tcc tat atc aag att tcc gat tcc acc tgc tca gcc acc acg ata cgc 307

Ser Tyr Ile Lys Ile Ser Asp Ser Thr Cys Ser Ala Thr Thr Ile Arg

55 60 65

acc cgc cga gac gag tgg atc act gcc ggg att ttc aag aat tta gaa 355

Thr Arg Arg Asp Glu Trp Ile Thr Ala Gly Ile Phe Lys Asn Leu Glu

70 75 80 85

cag atc tgt ctg gag tcc tac gac cgt ttc atc ggg tta gac cta gaa 403

Gln Ile Cys Leu Glu Ser Tyr Asp Arg Phe Ile Gly Leu Asp Leu Glu

90 95 100

aac tta aat gtt gat ggc tgc att gtt aaa gct ccc tgc ggc gga gag 451

Asn Leu Asn Val Asp Gly Cys Ile Val Lys Ala Pro Cys Gly Gly Glu

105 110 115

gta gcc ggc aga ttc ccg gtt gac cgg gaa aaa ggc acc aaa cgc tcg 499

Val Ala Gly Arg Phe Pro Val Asp Arg Glu Lys Gly Thr Lys Arg Ser

120 125 130

tta atg gtc gat gga cat gga atc ccg atc ggg tgc gtg gtc gcc gga 547

Leu Met Val Asp Gly His Gly Ile Pro Ile Gly Cys Val Val Ala Gly

135 140 145

gcc aat cgg cat gat tta ccg ttg tta gct gca acc ttg gac acg ctc 595

Ala Asn Arg His Asp Leu Pro Leu Leu Ala Ala Thr Leu Asp Thr Leu

150 155 160 165

ggc cgg ttt ggg ggc tct ctt ccc gat cag atc acg gtg cat ctc gat 643

Gly Arg Phe Gly Gly Ser Leu Pro Asp Gln Ile Thr Val His Leu Asp

170 175 180

gct ggg tat gac tcg aag aaa acc cgc agg cta ctc agc gaa ttt ggt 691

Ala Gly Tyr Asp Ser Lys Lys Thr Arg Arg Leu Leu Ser Glu Phe Gly

185 190 195

tat agc tgg gtg atc agc att aaa ggt gag ccg ctg cag gct ggg act 739

Tyr Ser Trp Val Ile Ser Ile Lys Gly Glu Pro Leu Gln Ala Gly Thr

200 205 210

cgg tgg gtg gtg gag cgt act aac tct tgg cat aac cgg ggt ttt aag 787

Arg Trp Val Val Glu Arg Thr Asn Ser Trp His Asn Arg Gly Phe Lys

215 220 225

aaa ctt agt atc tgc acc gaa cgt tgt acc cgg gtt gtg gaa gcg ttt 835

Lys Leu Ser Ile Cys Thr Glu Arg Cys Thr Arg Val Val Glu Ala Phe

230 235 240 245

atc gct tta gcc aac gcg gtg att att ctg cgt cgg ctt atc aaa cag 883

Ile Ala Leu Ala Asn Ala Val Ile Ile Leu Arg Arg Leu Ile Lys Gln

250 255 260

gcc tgg act agt tac cgc tgg gac acc cga ccg ggc cac aga cct 928

Ala Trp Thr Ser Tyr Arg Trp Asp Thr Arg Pro Gly His Arg Pro

265 270 275

taatctatcc gcgcaatctc taaggagaaa 958

<210>86

<211>276

<212>PRT

<213>谷氨酸棒状杆菌

<400>86

Val Pro Ala Leu Pro Ser Ser Ile Ile Asp Pro Leu Trp Arg Gln Phe

1 5 10 15

Ser Ala Leu Ile Pro Pro Val Ile Ile Thr His Pro Leu Gly Cys His

20 25 30

Arg Ala Arg Ile Ala Asp Arg Ile Ile Val Asp Lys Leu Ile Ala Val

35 40 45

Leu Val Leu Gly Val Ser Tyr Ile Lys Ile Ser Asp Ser Thr Cys Ser

50 55 60

Ala Thr Thr Ile Arg Thr Arg Arg Asp Glu Trp Ile Thr Ala Gly Ile

65 70 75 80

Phe Lys Asn Leu Glu Gln Ile Cys Leu Glu Ser Tyr Asp Arg Phe Ile

85 90 95

Gly Leu Asp Leu Glu Asn Leu Asn Val Asp Gly Cys Ile Val Lys Ala

100 105 110

Pro Cys Gly Gly Glu Val Ala Gly Arg Phe Pro Val Asp Arg Glu Lys

115 120 125

Gly Thr Lys Arg Ser Leu Met Val Asp Gly His Gly Ile Pro Ile Gly

130 135 140

Cys Val Val Ala Gly Ala Asn Arg His Asp Leu Pro Leu Leu Ala Ala

145 150 155 160

Thr Leu Asp Thr Leu Gly Arg Phe Gly Gly Ser Leu Pro Asp Gln Ile

165 170 175

Thr Val His Leu Asp Ala Gly Tyr Asp Ser Lys Lys Thr Arg Arg Leu

180 185 190

Leu Ser Glu Phe Gly Tyr Ser Trp Val Ile Ser Ile Lys Gly Glu Pro

195 200 205

Leu Gln Ala Gly Thr Arg Trp Val Val Glu Arg Thr Asn Ser Trp His

210 215 220

Asn Arg Gly Phe Lys Lys Leu Ser Ile Cys Thr Glu Arg Cys Thr Arg

225 230 235 240

Val Val Glu Ala Phe Ile Ala Leu Ala Asn Ala Val Ile Ile Leu Arg

245 250 255

Arg Leu Ile Lys Gln Ala Trp Thr Ser Tyr Arg Trp Asp Thr Arg Pro

260 265 270

Gly His Arg Pro

275

<210>87

<211>754

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(724)

<223>RXA03098

<400>87

gaccgttttg tcgatcgcac cgctgctggc tcgcaccatc aacgagatct tcgaaaacgg 60

ttccgtcacc accctcttcg agggcgaggc ctaaacaccc atg ccc acc acg gac 115

Met Pro Thr Thr Asp

1 5

gtc ttc aac cgc gtc cgg ttg gca ttg gaa cct cta gct gat ccc gca 163

Val Phe Asn Arg Val Arg Leu Ala Leu Glu Pro Leu Ala Asp Pro Ala

10 15 20

cgt gcc acc gga atg gca agc tac atg cgg gat cag ttt tct ttt ctc 211

Arg Ala Thr Gly Met Ala Ser Tyr Met Arg Asp Gln Phe Ser Phe Leu

25 30 35

ggc atc cca tcc acc ccc aga aaa gaa gcc tgc aaa ccc gtg ctg tcc 259

Gly Ile Pro Ser Thr Pro Arg Lys Glu Ala Cys Lys Pro Val Leu Ser

40 45 50

gcg cta aaa gag ttg gac act gac ttt gtc tca gac tgc ttt ggc gca 307

Ala Leu Lys Glu Leu Asp Thr Asp Phe Val Ser Asp Cys Phe Gly Ala

55 60 65

gct gaa cgg gaa tac cag tat gtc gcc tgc gat cac atc aat cgc gtc 355

Ala Glu Arg Glu Tyr Gln Tyr Val Ala Cys Asp His Ile Asn Arg Val

70 75 80 85

ggc atc acc gat cta ggt ttt gcc aaa gca tta gtg cag acc aaa tcc 403

Gly Ile Thr Asp Leu Gly Phe Ala Lys Ala Leu Val Gln Thr Lys Ser

90 95 100

tgg tgg gac acc gtc gat tcc cta gca aaa ccg atc ggc gcc aaa cac 451

Trp Trp Asp Thr Val Asp Ser Leu Ala Lys Pro Ile Gly Ala Lys His

105 110 115

gat gat gat ctg atg aaa acg tgg gcg ctt gat gag gac ttc tgg gtg 499

Asp Asp Asp Leu Met Lys Thr Trp Ala Leu Asp Glu Asp Phe Trp Val

120 125 130

cgc cgc atc gcg atc atc cac caa ctg ggc cgc aag aaa aac acc gac 547

Arg Arg Ile Ala Ile Ile His Gln Leu Gly Arg Lys Lys Asn Thr Asp

135 140 145

gct gcc ctg ctg gcc tgg atc atc gag cag aac ctc ggc tcc agc gag 595

Ala Ala Leu Leu Ala Trp Ile Ile Glu Gln Asn Leu Gly Ser Ser Glu

150 155 160 165

ttc ttc atc aac aaa gcg atc ggc tgg gca ctg cgg gat ttc gcc cgc 643

Phe Phe Ile Asn Lys Ala Ile Gly Trp Ala Leu Arg Asp Phe Ala Arg

170 175 180

cac gac ccc agc tgg gtc cgg gct ttt gtc gac gcc acg gac ctt tcc 691

His Asp Pro Ser Trp Val Arg Ala Phe Val Asp Ala Thr Asp Leu Ser

185 190 195

cca ctg agc cgg cga gaa gcc ctg aag aat att tagccctcag gcatcatctg 744

Pro Leu Ser Arg Arg Glu Ala Leu Lys Asn Ile

200 205

agcgagtgcc 754

<210>88

<211>208

<212>PRT

<213>谷氨酸棒状杆菌

<400>88

Met Pro Thr Thr Asp Val Phe Asn Arg Val Arg Leu Ala Leu Glu Pro

1 5 10 15

Leu Ala Asp Pro Ala Arg Ala Thr Gly Met Ala Ser Tyr Met Arg Asp

20 25 30

Gln Phe Ser Phe Leu Gly Ile Pro Ser Thr Pro Arg Lys Glu Ala Cys

35 40 45

Lys Pro Val Leu Ser Ala Leu Lys Glu Leu Asp Thr Asp Phe Val Ser

50 55 60

Asp Cys Phe Gly Ala Ala Glu Arg Glu Tyr Gln Tyr Val Ala Cys Asp

65 70 75 80

His Ile Asn Arg Val Gly Ile Thr Asp Leu Gly Phe Ala Lys Ala Leu

85 90 95

Val Gln Thr Lys Ser Trp Trp Asp Thr Val Asp Ser Leu Ala Lys Pro

100 105 110

Ile Gly Ala Lys His Asp Asp Asp Leu Met Lys Thr Trp Ala Leu Asp

115 120 125

Glu Asp Phe Trp Val Arg Arg Ile Ala Ile Ile His Gln Leu Gly Arg

130 135 140

Lys Lys Asn Thr Asp Ala Ala Leu Leu Ala Trp Ile Ile Glu Gln Asn

145 150 155 160

Leu Gly Ser Ser Glu Phe Phe Ile Asn Lys Ala Ile Gly Trp Ala Leu

165 170 175

Arg Asp Phe Ala Arg His Asp Pro Ser Trp Val Arg Ala Phe Val Asp

180 185 190

Ala Thr Asp Leu Ser Pro Leu Ser Arg Arg Glu Ala Leu Lys Asn Ile

195 200 205

<210>89

<211>562

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(532)

<223>RXA03206

<400>89

gaatccctgc acaacggcgc cattgcggcg ttggtggatc tcatccgcca cggattggtg 60

ttgcccgctg atcttctcga ttcttaaata aggactgatt gtg aaa gcc gtt tta 115

Val Lys Ala Val Leu

1 5

acc cgt gtg agt tcc gcc agc gtc agc gtg gat gat gaa att gtt gga 163

Thr Arg Val Ser Ser Ala Ser Val Ser Val Asp Asp Glu Ile Val Gly

10 15 20

gcc atc gat tgc ccc gac acc gga ggc att ttg gcg ctg gtt gga gtc 211

Ala Ile Asp Cys Pro Asp Thr Gly Gly Ile Leu Ala Leu Val Gly Val

25 30 35

ggc gct gct gat agc gac gac gcc tgg gaa acc atg gtg cga aaa att 259

Gly Ala Ala Asp Ser Asp Asp Ala Trp Glu Thr Met Val Arg Lys Ile

40 45 50

gct gag ctg cgc atc ttg gat ggc gaa caa tcc gtc agt gat gtc aat 307

Ala Glu Leu Arg Ile Leu Asp Gly Glu Gln Ser Val Ser Asp Val Asn

55 60 65

gct ccc gta ctg ctt gtt agc caa ttc acc ctg cat ggt cgc acc gca 355

Ala Pro Val Leu Leu Val Ser Gln Phe Thr Leu His Gly Arg Thr Ala

70 75 80 85

aaa ggc cgg cgc cca tcg tgg tct gat gca gca cct ggt gag gtg gct 403

Lys Gly Arg Arg Pro Ser Trp Ser Asp Ala Ala Pro Gly Glu Val Ala

90 95 100

gag ccg gtg att gaa aag att gca caa ggt tta cgt gag cgc gga atc 451

Glu Pro Val Ile Glu Lys Ile Ala Gln Gly Leu Arg Glu Arg Gly Ile

105 110 115

acc gtg gaa caa gga cga ttc ggc gca atg atg aag gtc aca tcg gtt 499

Thr Val Glu Gln Gly Arg Phe Gly Ala Met Met Lys Val Thr Ser Val

120 125 130

aac gaa ggc ccc ttc acc gtt ttg gtc gag tgc tagccagtca atcctaagag 552

Asn Glu Gly Pro Phe Thr Val Leu Val Glu cys

135 140

cttgaaacgc 562

<210>90

<211>144

<212>PRT

<213>谷氨酸棒状杆菌

<400>90

Val Lys Ala Val Leu Thr Arg Val Ser Ser Ala Ser Val Ser Val Asp

1 5 10 15

Asp Glu Ile Val Gly Ala Ile Asp Cys Pro Asp Thr Gly Gly Ile Leu

20 25 30

Ala Leu Val Gly Val Gly Ala Ala Asp Ser Asp Asp Ala Trp Glu Thr

35 40 45

Met Val Arg Lys Ile Ala Glu Leu Arg Ile Leu Asp Gly Glu Gln Ser

50 55 60

Val Ser Asp Val Asn Ala Pro Val Leu Leu Val Ser Gln Phe Thr Leu

65 70 75 80

His Gly Arg Thr Ala Lys Gly Arg Arg Pro Ser Trp Ser Asp Ala Ala

85 90 95

Pro Gly Glu Val Ala Glu Pro Val Ile Glu Lys Ile Ala Gln Gly Leu

100 105 110

Arg Glu Arg Gly Ile Thr Val Glu Gln Gly Arg Phe Gly Ala Met Met

115 120 125

Lys Val Thr Ser Val Asn Glu Gly Pro Phe Thr Val Leu Val Glu Cys

130 135 140

<210>91

<211>607

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(577)

<223>RXA03260

<400>91

cagatggctg gcagggatca gctgaccacg ttgctcaacc agcgtggtgt caaagtttct 60

actgggactg tgggatcaat tatgaacgaa tgaggagtgc gtg tca gac gaa tgc 115

Val Ser Asp Glu cys

1 5

ggg cct gga aga aca cca cgg tca gtg acc ctt tct gcc cgg acc gag 163

Gly Pro Gly Arg Thr Pro Arg Ser Val Thr Leu Ser Ala Arg Thr Glu

10 15 20

cat att aaa aat cat atg ctc gat agc cac ggg aaa cga gac ttt acc 211

His Ile Lys Asn His Met Leu Asp Ser His Gly Lys Arg Asp Phe Thr

25 30 35

gct acc gtg cct ggg acc agg ctc gtt ggt gac att acg tac tta aag 259

Ala Thr Val Pro Gly Thr Arg Leu Val Gly Asp Ile Thr Tyr Leu Lys

40 45 50

acg ggt tcc ggg tgg ctg tat gtg gct acc gtg atc gat ttg gct acg 307

Thr Gly Ser Gly Trp Leu Tyr Val Ala Thr Val Ile Asp Leu Ala Thr

55 60 65

cgg atg gtg gtg ggg tgg tct atg gat tct aat atg cgc aca ccg ttg 355

Arg Met Val Val Gly Trp Ser Met Asp Ser Asn Met Arg Thr Pro Leu

70 75 80 85

gtg atc aat gcg ctg gct atg gcg cgt gat cat ggg tgt ctt cat cct 403

Val Ile Asn Ala Leu Ala Met Ala Arg Asp His Gly Cys Leu His Pro

90 95 100

gaa ggc gca att ttt cac tcc gat aga gga tcg caa tac acc tcc gag 451

Glu Gly Ala Ile Phe His Ser Asp Arg Gly Ser Gln Tyr Thr Ser Glu

105 110 115

cag ttc cag aca tgg tgc gcc ggc aac aag atc acc caa tcc atg gga 499

Gln Phe Gln Thr Trp Cys Ala Gly Asn Lys Ile Thr Gln Ser Met Gly

120 125 130

ttg acc ggg gtg tgt tgg gat aac gga agt cgc gga gaa ttt ttt ctc 547

Leu Thr Gly Val Cys Trp Asp Asn Gly Ser Arg Gly Glu Phe Phe Leu

135 140 145

aca ttt gaa gac cga aat gta tca cca cta tgattttgag aatcacctgt 597

Thr Phe Glu Asp Arg Asn Val Ser Pro Leu

150 155

cggaccgaac 607

<210>92

<211>159

<212>PRT

<213>谷氨酸棒状杆菌

<400>92

Val Ser Asp Glu Cys Gly Pro Gly Arg Thr Pro Arg Ser Val Thr Leu

1 5 10 15

Ser Ala Arg Thr Glu His Ile Lys Asn His Met Leu Asp Ser His Gly

20 25 30

Lys Arg Asp Phe Thr Ala Thr Val Pro Gly Thr Arg Leu Val Gly Asp

35 40 45

Ile Thr Tyr Leu Lys Thr Gly Ser Gly Trp Leu Tyr Val Ala Thr Val

50 55 60

Ile Asp Leu Ala Thr Arg Met Val Val Gly Trp Ser Met Asp Ser Asn

65 70 75 80

Met Arg Thr Pro Leu Val Ile Asn Ala Leu Ala Met Ala Arg Asp His

85 90 95

Gly Cys Leu His Pro Glu Gly Ala Ile Phe His Ser Asp Arg Gly Ser

100 105 110

Gln Tyr Thr Ser Glu Gln Phe Gln Thr Trp Cys Ala Gly Asn Lys Ile

115 120 125

Thr Gln Ser Met Gly Leu Thr Gly Val cys Trp Asp Asn Gly Ser Arg

130 135 140

Gly Glu Phe Phe Leu Thr Phe Glu Asp Arg Asn Val Ser Pro Leu

145 150 155

<210>93

<211>1969

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1939)

<223>RXA03394

<400>93

ctgcaaagcg acgcagggag cgtaaggcga gtggcgcggg gaagcgtcga taggcaattt 60

ttaacccctg ataccccttt ccggccgggc ataaattaag gtg gta cgc atg acg 115

Val Val Arg Met Thr

1 5

aag aac gtg ctc gta tct gtt gcc tgg ccg tat gcc aac gga ccc cgt 163

Lys Asn Val Leu Val Ser Val Ala Trp Pro Tyr Ala Asn Gly Pro Arg

10 15 20

cac att gga cat gtg gcg ggg ttt ggt gtc ccc tcc gat gtg ttc gca 211

His Ile Gly His Val Ala Gly Phe Gly Val Pro Ser Asp Val Phe Ala

25 30 35

agg ttc cag cga atg tct ggc aac aac gtg ctc atg gtg tcc ggc acc 259

Arg Phe Gln Arg Met Ser Gly Asn Asn Val Leu Met Val Ser Gly Thr

40 45 50

gat gag cac ggc acg cca ctt ctg gtt caa gca gac aaa gaa ggc gtc 307

Asp Glu His Gly Thr Pro Leu Leu Val Gln Ala Asp Lys Glu Gly Val

55 60 65

acc gtt caa gac cta gcg gat aag tac aac cgc cag atc gtc gaa gac 355

Thr Val Gln Asp Leu Ala Asp Lys Tyr Asn Arg Gln Ile Val Glu Asp

70 75 80 85

ctc acc ggc ctg ggc ctg tcc tat gac ctt ttc acc cgc acc acc acc 403

Leu Thr Gly Leu Gly Leu Ser Tyr Asp Leu Phe Thr Arg Thr Thr Thr

90 95 100

tcc aac cac tac gca gta gtg cag gaa ctg ttc cgt ggt ctg tac gac 451

Ser Asn His Tyr Ala Val Val Gln Glu Leu Phe Arg Gly Leu Tyr Asp

105 110 115

aac ggt tac atg atc aag gaa acc acc ctc ggt gcg att tcc cca tcc 499

Asn Gly Tyr Met Ile Lys Glu Thr Thr Leu Gly Ala Ile Ser Pro Ser

120 125 130

act ggc cgt acc ctg cca gac cgc tac att gaa ggc acc tgc cca atc 547

Thr Gly Arg Thr Leu Pro Asp Arg Tyr Ile Glu Gly Thr Cys Pro Ile

135 140 145

tgt ggc acc gac ggc gct cgt ggc gac cag tgc gac aac tgc gga aac 595

Cys Gly Thr Asp Gly Ala Arg Gly Asp Gln Cys Asp Asn Cys Gly Asn

150 155 160 165

cag ctc gat cca gcg gac ctg atc aac ccg gtg tcc aag atc aac ggc 643

Gln Leu Asp Pro Ala Asp Leu Ile Asn Pro Val Ser Lys Ile Asn Gly

170 175 180

gaa acc cca gag ttc gtt gag acc gaa cac ttc ctg ctc gac ctg cca 691

Glu Thr Pro Glu Phe Val Glu Thr Glu His Phe Leu Leu Asp Leu Pro

185 190 195

gca ctg gct gaa gca cta acc gag tgg ctg aag gga cgc gaa gac tgg 739

Ala Leu Ala Glu Ala Leu Thr Glu Trp Leu Lys Gly Arg Glu Asp Trp

200 205 210

cgt cca aac gtg ttg aag ttc tcg ctc aac ctg ctg gac gat atc cgc 787

Arg Pro Asn Val Leu Lys Phe Ser Leu Asn Leu Leu Asp Asp Ile Arg

215 220 225

cca cgc gca atg tcg cgc gat atc gac tgg ggc atc cca atc cca gtt 835

Pro Arg Ala Met Ser Arg Asp Ile Asp Trp Gly Ile Pro Ile Pro Val

230 235 240 245

gaa gga tgg caa gac aac aac gcc aag aag ctc tac gtc tgg ttc gac 883

Glu Gly Trp Gln Asp Asn Asn Ala Lys Lys Leu Tyr Val Trp Phe Asp

250 255 260

gct gtc gtg ggc tac ttg tcc gca tcc atc gaa tgg gcc tac cgc tcc 931

Ala Val Val Gly Tyr Leu Ser Ala Ser Ile Glu Trp Ala Tyr Arg Ser

265 270 275

ggc gac cca gaa gca tgg cgc acc ttc tgg aat gat cca gaa acc aag 979

Gly Asp Pro Glu Ala Trp Arg Thr Phe Trp Asn Asp Pro Glu Thr Lys

280 285 290

tcc tac tac ttc atg ggc aaa gac aac atc acc ttc cac tcc cag atc 1027

Ser Tyr Tyr Phe Met Gly Lys Asp Asn Ile Thr Phe His Ser Gln Ile

295 300 305

tgg cca gcg gag ctt ctc ggc tac gca ggc aag ggc tcc cgc ggt gga 1075

Trp Pro Ala Glu Leu Leu Gly Tyr Ala Gly Lys Gly Ser Arg Gly Gly

310 315 320 325

gaa atc ggt gac ctg ggt gtt ctg aac ctg cct act gag gtt gtt tcc 1123

Glu Ile Gly Asp Leu Gly Val Leu Asn Leu Pro Thr Glu Val Val Ser

330 335 340

tct gag ttc ctg act atg tct gga tcc aag ttc tcc tca tcc aag ggc 1171

Ser Glu Phe Leu Thr Met Ser Gly Ser Lys Phe Ser Ser Ser Lys Gly

345 350 355

gtt gtc atc tac gtg aag gac ttc ctc aag gag ttc ggc cca gat gcg 1219

Val Val Ile Tyr Val Lys Asp Phe Leu Lys Glu Phe Gly Pro Asp Ala

360 365 370

ctg cga tac ttc atc gct gtc gca ggc cca gaa aac aac gac acc gac 1267

Leu Arg Tyr Phe Ile Ala Val Ala Gly Pro Glu Asn Asn Asp Thr Asp

375 380 385

ttc acc tgg gat gaa ttt gtc cgc cgc gta aat aac gag ctg gca aac 1315

Phe Thr Trp Asp Glu Phe Val Arg Arg Val Asn Asn Glu Leu Ala Asn

390 395 400 405

ggc tgg ggc aac ctg gtc aac cgc act gta tcc atg gcg cac aag aac 1363

Gly Trp Gly Asn Leu Val Asn Arg Thr Val Ser Met Ala His Lys Asn

410 415 420

ttc ggt gaa gta cca gta cct ggc gca ctg gaa gaa tct gac aag aag 1411

Phe Gly Glu Val Pro Val Pro Gly Ala Leu Glu Glu Ser Asp Lys Lys

425 430 435

atc ctt gat ctt gct acc gct gcc ttt gaa tcc gtt gct gcg aac ctg 1459

Ile Leu Asp Leu Ala Thr Ala Ala Phe Glu Ser Val Ala Ala Asn Leu

440 445 450

gat cag tcc aag ttc aag gcc ggt atc tct gaa atc atg cac gtt gtc 1507

Asp Gln Ser Lys Phe Lys Ala Gly Ile Ser Glu Ile Met His Val Val

455 460 465

ggt gag gcc aac gcc tac atc gca gag caa gaa cca tgg aag ctt gcc 1555

Gly Glu Ala Asn Ala Tyr Ile Ala Glu Gln Glu Pro Trp Lys Leu Ala

470 475 480 485

aag gat gac acc aag cgc gag cgt ctt gcc acc gtg ctg tgg act gcg 1603

Lys Asp Asp Thr Lys Arg Glu Arg Leu Ala Thr Val Leu Trp Thr Ala

490 495 500

ctg cag gtt gtt tct gac tgc aac acc atg ctg acc cca tac ctg cca 1651

Leu Gln Val Val Ser Asp Cys Asn Thr Met Leu Thr Pro Tyr Leu Pro

505 510 515

cac acc gcc caa aag gtg cat gag acc ttg ggc cgt gat gga atc tgg 1699

His Thr Ala Gln Lys Val His Glu Thr Leu Gly Arg Asp Gly Ile Trp

520 525 530

gct gca aca cca cag atc gtg gaa gtc acc aac gaa tca cca cgc cag 1747

Ala Ala Thr Pro Gln Ile Val Glu Val Thr Asn Glu Ser Pro Arg Gln

535 540 545

cca atc ggc gtg ggg cta cca gat cca gag cac acc tac cca gta atc 1795

Pro Ile Gly Val Gly Leu Pro Asp Pro Glu His Thr Tyr Pro Val Ile

550 555 560 565

atg ggc gac tac aag acc cag ctg gct aag tgg cag cgc atc gac gtt 1843

Met Gly Asp Tyr Lys Thr Gln Leu Ala Lys Trp Gln Arg Ile Asp Val

570 575 580

gtg cca ggc acc acc ttg gag aag cca gca ccg ctg att gct aag ctc 1891

Val Pro Gly Thr Thr Leu Glu Lys Pro Ala Pro Leu Ile Ala Lys Leu

585 590 595

gat cca gaa ctt ggt gaa acc ggc cca gaa tgg gca cca gtg cag aac 1939

Asp Pro Glu Leu Gly Glu Thr Gly Pro Glu Trp Ala Pro Val Gln Asn

600 605 610

taaagcatct ttagcatgaa ccgagcaggt 1969

<210>94

<211>613

<212>PRT

<213>谷氨酸棒状杆菌

<400>94

Val Val Arg Met Thr Tys Asn Val Leu Val Ser Val Ala Trp Pro Tyr

1 5 10 15

Ala Asn Gly Pro Arg His Ile Gly His Val Ala Gly Phe Gly Val Pro

20 25 30

Ser Asp Val Phe Ala Arg Phe Gln Arg Met Ser Gly Asn Asn Val Leu

35 40 45

Met Val Ser Gly Thr Asp Glu His Gly Thr Pro Leu Leu Val Gln Ala

50 55 60

Asp Lys Glu Gly Val Thr Val Gln Asp Leu Ala Asp Lys Tyr Asn Arg

65 70 75 80

Gln Ile Val Glu Asp Leu Thr Gly Leu Gly Leu Ser Tyr Asp Leu Phe

85 90 95

Thr Arg Thr Thr Thr Ser Asn His Tyr Ala Val Val Gln Glu Leu Phe

100 105 110

Arg Gly Leu Tyr Asp Asn Gly Tyr Met Ile Lys Glu Thr Thr Leu Gly

115 120 125

Ala Ile Ser Pro Ser Thr Gly Arg Thr Leu Pro Asp Arg Tyr Ile Glu

130 135 140

Gly Thr Cys Pro Ile Cys Gly Thr Asp Gly Ala Arg Gly Asp Gln Cys

145 150 155 160

Asp Asn Cys Gly Asn Gln Leu Asp Pro Ala Asp Leu Ile Asn Pro Val

165 170 175

Ser Lys Ile Asn Gly Glu Thr Pro Glu Phe Val Glu Thr Glu His Phe

180 185 190

Leu Leu Asp Leu Pro Ala Leu Ala Glu Ala Leu Thr Glu Trp Leu Lys

195 200 205

Gly Arg Glu Asp Trp Arg Pro Asn Val Leu Lys Phe Ser Leu Asn Leu

210 215 220

Leu Asp Asp Ile Arg Pro Arg Ala Met Ser Arg Asp Ile Asp Trp Gly

225 230 235 240

Ile Pro Ile Pro Val Glu Gly Trp Gln Asp Asn Asn Ala Lys Lys Leu

245 250 255

Tyr Val Trp Phe Asp Ala Val Val Gly Tyr Leu Ser Ala Ser Ile Glu

260 265 270

Trp Ala Tyr Arg Ser Gly Asp Pro Glu Ala Trp Arg Thr Phe Trp Asn

275 280 285

Asp Pro Glu Thr Lys Ser Tyr Tyr Phe Met Gly Lys Asp Asn Ile Thr

290 295 300

Phe His Ser Gln Ile Trp Pro Ala Glu Leu Leu Gly Tyr Ala Gly Lys

305 310 315 320

Gly Ser Arg Gly Gly Glu Ile Gly Asp Leu Gly Val Leu Asn Leu Pro

325 330 335

Thr Glu Val Val Ser Ser Glu Phe Leu Thr Met Ser Gly Ser Lys Phe

340 345 350

Ser Ser Ser Lys Gly Val Val Ile Tyr Val Lys Asp Phe Leu Lys Glu

355 360 365

Phe Gly Pro Asp Ala Leu Arg Tyr Phe Ile Ala Val Ala Gly Pro Glu

370 375 380

Asn Asn Asp Thr Asp Phe Thr Trp Asp Glu Phe Val Arg Arg Val Asn

385 390 395 400

Asn Glu Leu Ala Asn Gly Trp Gly Asn Leu Val Asn Arg Thr Val Ser

405 410 415

Met Ala His Lys Asn Phe Gly Glu Val Pro Val Pro Gly Ala Leu Glu

420 425 430

Glu Ser Asp Lys Lys Ile Leu Asp Leu Ala Thr Ala Ala Phe Glu Ser

435 440 445

Val Ala Ala Asn Leu Asp Gln Ser Lys Phe Lys Ala Gly Ile Ser Glu

450 455 460

Ile Met His Val Val Gly Glu Ala Asn Ala Tyr Ile Ala Glu Gln Glu

465 470 475 480

Pro Trp Lys Leu Ala Lys Asp Asp Thr Lys Arg Glu Arg Leu Ala Thr

485 490 495

Val Leu Trp Thr Ala Leu Gln Val Val Ser Asp Cys Asn Thr Met Leu

500 505 510

Thr Pro Tyr Leu Pro His Thr Ala Gln Lys Val His Glu Thr Leu Gly

515 520 525

Arg Asp Gly Ile Trp Ala Ala Thr Pro Gln Ile Val Glu Val Thr Asn

530 535 540

Glu Ser Pro Arg Gln Pro Ile Gly Val Gly Leu Pro Asp Pro Glu His

545 550 555 560

Thr Tyr Pro Val Ile Met Gly Asp Tyr Lys Thr Gln Leu Ala Lys Trp

565 570 575

Gln Arg Ile Asp Val Val Pro Gly Thr Thr Leu Glu Lys Pro Ala Pro

580 585 590

Leu Ile Ala Lys Leu Asp Pro Glu Leu Gly Glu Thr Gly Pro Glu Trp

595 600 605

Ala Pro Val Gln Asn

610

<210>95

<211>3016

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2986)

<223>RXA03674

<400>95

tccccgcagc ccaccaccgt gggctgcggg gtgtggcgtt tttgccacaa agtggaccgt 60

attcgcaaat actttgttaa gacgcgttaa tctttaacct atg tct gaa tca ggt 115

Met Ser Glu Ser Gly

1 5

gcg cta agt tct act gac tct cta tcc ccg ggt gtc acc att gaa gtc 163

Ala Leu Ser Ser Thr Asp Ser Leu Ser Pro Gly Val Thr Ile Glu Val

10 15 20

cga gat gaa att tgg ctg gtt act cac gtt act cgc tcc aca gat ggt 211

Arg Asp Glu Ile Trp Leu Val Thr His Val Thr Arg Ser Thr Asp Gly

25 30 35

ttt agg gtt aaa gct cgt ggt ctc tct gat tat gtg cgg gac cac gaa 259

Phe Arg Val Lys Ala Arg Gly Leu Ser Asp Tyr Val Arg Asp His Glu

40 45 50

gct acg ttc ttc acc gca ctt gat aaa gat ttg aag gtc att gac cct 307

Ala Thr Phe Phe Thr Ala Leu Asp Lys Asp Leu Lys Val Ile Asp Pro

55 60 65

acc cag gtc acc gtc agt ctt gat gat tcc tcc aat tac cgt cgc acc 355

Thr Gln Val Thr Val Ser Leu Asp Asp Ser Ser Asn Tyr Arg Arg Thr

70 75 80 85

cgc ctg tgg ttg gag gcc acc atg cgt aaa act ccg gta ccg ctc tat 403

Arg Leu Trp Leu Glu Ala Thr Met Arg Lys Thr Pro Val Pro Leu Tyr

90 95 100

caa gag tca ctt tcc gtg gca gat caa atg ctc gcc gat cca ctg gag 451

Gln Glu Ser Leu Ser Val Ala Asp Gln Met Leu Ala Asp Pro Leu Glu

105 110 115

tac caa tta gca gcc gtg cgc aaa acc ctc tct agt gct aac ttg cgc 499

Tyr Gln Leu Ala Ala Val Arg Lys Thr Leu Ser Ser Ala Asn Leu Arg

120 125 130

ccc cgc gtg ctt att gct gat gcc gtg gga ctt ggc aaa acc cta gaa 547

Pro Arg Val Leu Ile Ala Asp Ala Val Gly Leu Gly Lys Thr Leu Glu

135 140 145

atg ggc atg atc ttg gcg gaa ctt atc cgc cgt ggc cgt ggt gag cgc 595

Met Gly Met Ile Leu Ala Glu Leu Ile Arg Arg Gly Arg Gly Glu Arg

150 155 160 165

att ttg gta gtc acc ccg cgc cac att atg gag cag ttc cag cag gaa 643

Ile Leu Val Val Thr Pro Arg His Ile Met Glu Gln Phe Gln Gln Glu

170 175 180

atg tgg acc cgt ttt gcc atc ccg ctc gtt cgt cta gat tcc gtg ggc 691

Met Trp Thr Arg Phe Ala Ile Pro Leu Val Arg Leu Asp Ser Val Gly

185 190 195

atc cag caa gtg cgc caa aaa ttg cca gca tca cgc aac cct ttt act 739

Ile Gln Gln Val Arg Gln Lys Leu Pro Ala Ser Arg Asn Pro Phe Thr

200 205 210

tat ttc ccg cgc gtg att gtc tct atg gat act ttg aaa tct ccg aag 787

Tyr Phe Pro Arg Val Ile Val Ser Met Asp Thr Leu Lys Ser Pro Lys

215 220 225

tac cgc gcg caa cta gaa aag gtg cac tgg gat gcg gtg gtt ata gat 835

Tyr Arg Ala Gln Leu Glu Lys Val His Trp Asp Ala Val Val Ile Asp

230 235 240 245

gaa atc cac aat gca acc aat gct ggc acc caa aat aat gag cta gcc 883

Glu Ile His Asn Ala Thr Asn Ala Gly Thr Gln Asn Asn Glu Leu Ala

250 255 260

cgc aca ctt gcg cct act gcc gag gct ctt att ttg gcc tct gcc acc 931

Arg Thr Leu Ala Pro Thr Ala Glu Ala Leu Ile Leu Ala Ser Ala Thr

265 270 275

ccg cac aat ggt gat cca gaa tcc ttt aag gag atc ttg cgt ttg ctt 979

Pro His Asn Gly Asp Pro Glu Ser Phe Lys Glu Ile Leu Arg Leu Leu

280 285 290

gat ccc acc gct gtg atg cct gat ggc acc att gat gcc gaa gct gca 1027

Asp Pro Thr Ala Val Met Pro Asp Gly Thr Ile Asp Ala Glu Ala Ala

295 300 305

cag cgt ctg atc att cgt cgc cat cgc aat agc cct gag gtt tca ggt 1075

Gln Arg Leu Ile Ile Arg Arg His Arg Asn Ser Pro Glu Val Ser Gly

310 315 320 325

ttt gtg ggc gaa aaa tgg gct cca cgc aat gag cct cag aac ttc ctg 1123

Phe Val Gly Glu Lys Trp Ala Pro Arg Asn Glu Pro Gln Asn Phe Leu

330 335 340

gtc gct gcg tca aaa gaa gaa aac ggc gtt gct gca gaa ctc aac cat 1171

Val Ala Ala Ser Lys Glu Glu Asn Gly Val Ala Ala Glu Leu Asn His

345 350 355

gtg tgg att tca cca ggt gcg agc aat ccg atc aag gat cgc ctc ttc 1219

Val Trp Ile Ser Pro Gly Ala Ser Asn Pro Ile Lys Asp Arg Leu Phe

360 365 370

ccc tgg aca ttg gtg aag gct ttt ctc tcc tcc cct gca gcc ttg ggc 1267

Pro Trp Thr Leu Val Lys Ala Phe Leu Ser Ser Pro Ala Ala Leu Gly

375 380 385

gaa aca gtg tcc aat cgc ctc aaa aag gcc tct gca cca gaa gaa aaa 1315

Glu Thr Val Ser Asn Arg Leu Lys Lys Ala Ser Ala Pro Glu Glu Lys

390 395 400 405

cgc gcc cta gaa acc ctt tca caa ctt aat tct gcg atc acc ccg cag 1363

Arg Ala Leu Glu Thr Leu Ser Gln Leu Asn Ser Ala Ile Thr Pro Gln

410 415 420

acc tca cag aag tac caa tct cta ctg agc tac ctc ggt gac atc gga 1411

Thr Ser Gln Lys Tyr Gln Ser Leu Leu Ser Tyr Leu Gly Asp Ile Gly

425 430 435

gtg aag aag aac tcc gat acc cgc gtg gtg att ttc tct gag cgt gtc 1459

Val Lys Lys Asn Ser Asp Thr Arg Val Val Ile Phe Ser Glu Arg Val

440 445 450

gct act ttg cac tgg ctg cag gaa aac ctc atc cgt gat ctc aag atg 1507

Ala Thr Leu His Trp Leu Gln Glu Asn Leu Ile Arg Asp Leu Lys Met

455 460 465

cca ccc aac tct att gct gtt atg cac ggc ggt ctc ccc gac cag gag 1555

Pro Pro Asn Ser Ile Ala Val Met His Gly Gly Leu Pro Asp Gln Glu

470 475 480 485

cas atg cgc ctg gtg gat gag ttt aaa aag acg gat tct ccc atc cgc 1603

Gln Met Arg Leu Val Asp Glu Phe Lys Lys Thr Asp Ser Pro Ile Arg

490 495 500

atc atg atc acc ggc gat gtt gcc tca gaa ggt gtg aac ctg cat act 1651

Ile Met Ile Thr Gly Asp Val Ala Ser Glu Gly Val Asn Leu His Thr

505 510 515

ctc tgc cac aac ttg gtg cac tat gac atc ccg tgg tca ctg atc cgc 1699

Leu Cys His Asn Leu Val His Tyr Asp Ile Pro Trp Ser Leu Ile Arg

520 525 530

att cag cag cgc aat ggc cgt att gat cgt tat ggt caa acc cac aac 1747

Ile Gln Gln Arg Asn Gly Arg Ile Asp Arg Tyr Gly Gln Thr His Asn

535 540 545

cct tcc atc gtt acc ttc ttg ctc gat ccc gcc gag gat tcc aaa gta 1795

Pro Ser Ile Val Thr Phe Leu Leu Asp Pro Ala Glu Asp Ser Lys Val

550 555 560 565

ggt gaa gtc cat gtg ctg gag agg ctc atg gag cgc gaa cat gag gcg 1843

Gly Glu Val His Val Leu Glu Arg Leu Met Glu Arg Glu His Glu Ala

570 575 580

cac tct ttg ctc ggt gat gcc gca tct ctc atg ggc aag cac tct gag 1891

His Ser Leu Leu Gly Asp Ala Ala Ser Leu Met Gly Lys His Ser Glu

585 590 595

cgt ttg gaa gaa gaa acc atc cgc gaa gtc ctg cgc ggt gcc caa aac 1939

Arg Leu Glu Glu Glu Thr Ile Arg Glu Val Leu Arg Gly Ala Gln Asn

600 605 610

ttt aat gat gca gtg gct gat cca gcg gaa gtc cta gaa aac cca gca 1987

Phe Asn Asp Ala Val Ala Asp Pro Ala Glu Val Leu Glu Asn Pro Ala

615 620 625

ggc cta gat gat att gat tgg ttg cta gcc caa atc gcc caa gcc gat 2035

Gly Leu Asp Asp Ile Asp Trp Leu Leu Ala Gln Ile Ala Gln Ala Asp

630 635 640 645

gcc aag gca gaa aca gaa gca gaa gca gaa aca gaa aac caa aca gca 2083

Ala Lys Ala Glu Thr Glu Ala Glu Ala Glu Thr Glu Asn Gln Thr Ala

650 655 660

cca gat gca gct tcc aat agc acg cag cat gca caa cgc cgg ttg tat 2131

Pro Asp Ala Ala Ser Asn Ser Thr Gln His Ala Gln Arg Arg Leu Tyr

665 670 675

gca cag gaa agc tct ttc ctc tat gac tgc ctc ctc gaa ggt ttc aat 2179

Ala Gln Glu Ser Ser Phe Leu Tyr Asp Cys Leu Leu Glu Gly Phe Asn

680 685 690

aac gta ccg gag gat tcc atc aac cgc ggt ggc gtg ggg ttc aaa aaa 2227

Asn Val Pro Glu Asp Ser Ile Asn Arg Gly Gly Val Gly Phe Lys Lys

695 700 705

cac gat aat gac atc gtg gag ctc acc ccc acc gat gat ctg cgc cgt 2275

His Asp Asn Asp Ile Val Glu Leu Thr Pro Thr Asp Asp Leu Arg Arg

710 715 720 725

cgt cta gat ttc ctc ccg cag gat tat gtg gct gcc cgg aaa gtt aag 2323

Arg Leu Asp Phe Leu Pro Gln Asp Tyr Val Ala Ala Arg Lys Val Lys

730 735 740

gaa gat ctc cta cta gct tcc aca ctg atg cgt ggc caa gaa cgc ctc 2371

Glu Asp Leu Leu Leu Ala Ser Thr Leu Met Arg Gly Gln Glu Arg Leu

745 750 755

aac gct gcg cgc act ggt gaa gat ggc agt acc tgg cca agt gcc cac 2419

Asr Ala Ala Arg Thr Gly Glu Asp Gly Ser Thr Trp Pro Ser Ala His

760 765 770

tat cta ggc ccc ctg cac cca gtc act tcg tgg gca gct gac cgc gcg 2467

Tyr Leu Gly Pro Leu His Pro Val Thr Ser Trp Ala Ala Asp Arg Ala

775 780 785

ctg gca acc atg cca cgt tcg gaa att ccg gcg gct agt ggc aaa gtc 2515

Leu Ala Thr Met Pro Arg Ser Glu Ile Pro Ala Ala Ser Gly Lys Val

790 795 800 805

aca gag cca acg gtg ctg ctt atg tcc aca ttg agc aat cgg cgt ggc 2563

Thr Glu Pro Thr Val Leu Leu Met Ser Thr Leu Ser Asn Arg Arg Gly

810 815 820

caa att gtg tct cgt tct ttt gtg gct tct tct ggc ccc ttt gat act 2611

Gln Ile Val Ser Arg Ser Phe Val Ala Ser Ser Gly Pro Phe Asp Thr

825 830 835

gag gtg ctg tcc gat ccc atc caa tgg tta cat tcc ata ggc ctc gat 2659

Glu Val Leu Ser Asp Pro Ile Gln Trp Leu His Ser Ile Gly Leu Asp

840 845 850

gaa acc gcc att aac cca ggt acc gct gca ctc ccc gac gat att gag 2707

Glu Thr Ala Ile Asn Pro Gly Thr Ala Ala Leu Pro Asp Asp Ile Glu

855 860 865

cag ctt att tcc ctt gct gtt cag gcc gcc cgc ggc gag atc cgt cca 2755

Gln Leu Ile Ser Leu Ala Val Gln Ala Ala Arg Gly Glu Ile Arg Pro

870 875 880 885

tta atg atc gcc gcc cgc gct cag gct caa act cgc gtt gag cat tgg 2803

Leu Met Ile Ala Ala Arg Ala Gln Ala Gln Thr Arg Val Glu His Trp

890 895 900

gct aag cga gcc gaa gcc tgg aat aac aaa cga agt ggc gca gcg tcc 2851

Ala Lys Arg Ala Glu Ala Trp Asn Asn Lys Arg Ser Gly Ala Ala Ser

905 910 915

acg tcc cgt acc gcg cga act gca aaa ttg att gag gag cag cag aaa 2899

Thr Ser Arg Thr Ala Arg Thr Ala Lys Leu Ile Glu Glu Gln Gln Lys

920 925 930

ttg agt aat gct ctc gag cca gac cgt gaa ctt att agg cct ttg gcc 2947

Leu Ser Asn Ala Leu Glu Pro Asp Arg Glu Leu Ile Arg Pro Leu Ala

935 940 945

gtc att ctt ccg cag ccc gca act ttg aac acc gag gtt taacacaatg 2996

Val Ile Leu Pro Gln Pro Ala Thr Leu Asn Thr Glu Val

950 955 960

agtgcatttg attcgatcct 3016

<210>96

<211>962

<212>PRT

<213>谷氨酸棒状杆菌

<400>96

Met Ser Glu Ser Gly Ala Leu Ser Ser Thr Asp Ser Leu Ser Pro Gly

1 5 10 15

Val Thr Ile Glu Val Arg Asp Glu Ile Trp Leu Val Thr His Val Thr

20 25 30

Arg Ser Thr Asp Gly Phe Arg Val Lys Ala Arg Gly Leu Ser Asp Tyr

35 40 45

Val Arg Asp His Glu Ala Thr Phe Phe Thr Ala Leu Asp Lys Asp Leu

50 55 60

Lys Val Ile Asp Pro Thr Gln Val Thr Val Ser Leu Asp Asp Ser Ser

65 70 75 80

Asn Tyr Arg Arg Thr Arg Leu Trp Leu Glu Ala Thr Met Arg Lys Thr

85 90 95

Pro Val Pro Leu Tyr Gln Glu Ser Leu Ser Val Ala Asp Gln Met Leu

100 105 110

Ala Asp Pro Leu Glu Tyr Gln Leu Ala Ala Val Arg Lys Thr Leu Ser

115 120 125

Ser Ala Asn Leu Arg Pro Arg Val Leu Ile Ala Asp Ala Val Gly Leu

130 135 140

Gly Lys Thr Leu Glu Met Gly Met Ile Leu Ala Glu Leu Ile Arg Arg

145 150 155 160

Gly Arg Gly Glu Arg Ile Leu Val Val Thr Pro Arg His Ile Met Glu

165 170 175

Gln Phe Gln Gln Glu Met Trp Thr Arg Phe Ala Ile Pro Leu Val Arg

180 185 190

Leu Asp Ser Val Gly Ile Gln Gln Val Arg Gln Lys Leu Pro Ala Ser

195 200 205

Arg Asn Pro Phe Thr Tyr Phe Pro Arg Val Ile Val Ser Met Asp Thr

210 215 220

Leu Lys Ser Pro Lys Tyr Arg Ala Gln Leu Glu Lys Val His Trp Asp

225 230 235 240

Ala Val Val Ile Asp Glu Ile His Asn Ala Thr Asn Ala Gly Thr Gln

245 250 255

Asn Asn Glu Leu Ala Arg Thr Leu Ala Pro Thr Ala Glu Ala Leu Ile

260 265 270

Leu Ala Ser Ala Thr Pro His Asn Gly Asp Pro Glu Ser Phe Lys Glu

275 280 285

Ile Leu Arg Leu Leu Asp Pro Thr Ala Val Met Pro Asp Gly Thr Ile

290 295 300

Asp Ala Glu Ala Ala Gln Arg Leu Ile Ile Arg Arg His Arg Asn Ser

305 310 315 320

Pro Glu Val Ser Gly Phe Val Gly Glu Lys Trp Ala Pro Arg Asn Glu

325 330 335

Pro Gln Asn Phe Leu Val Ala Ala Ser Lys Glu Glu Asn Gly Val Ala

340 345 350

Ala Glu Leu Asn His Val Trp Ile Ser Pro Gly Ala Ser Asn Pro Ile

355 360 365

Lys Asp Arg Leu Phe Pro Trp Thr Leu Val Lys Ala Phe Leu Ser Ser

370 375 380

Pro Ala Ala Leu Gly Glu Thr Val Ser Asn Arg Leu Lys Lys Ala Ser

385 390 395 400

Ala Pro Glu Glu Lys Arg Ala Leu Glu Thr Leu Ser Gln Leu Asn Ser

405 410 415

Ala Ile Thr Pro Gln Thr Ser Gln Lys Tyr Gln Ser Leu Leu Ser Tyr

420 425 430

Leu Gly Asp Ile Gly Val Lys Lys Asn Ser Asp Thr Arg Val Val Ile

435 440 445

Phe Ser Glu Arg Val Ala Thr Leu His Trp Leu Gln Glu Asn Leu Ile

450 455 460

Arg Asp Leu Lys Met Pro Pro Asn Ser Ile Ala Val Met His Gly Gly

465 470 475 480

Leu Pro Asp Gln Glu Gln Met Arg Leu Val Asp Glu Phe Lys Lys Thr

485 490 495

Asp Ser Pro Ile Arg Ile Met Ile Thr Gly Asp Val Ala Ser Glu Gly

500 505 510

Val Asn Leu His Thr Leu Cys His Asn Leu Val His Tyr Asp Ile Pro

515 520 525

Trp Ser Leu Ile Arg Ile Gln Gln Arg Asn Gly Arg Ile Asp Arg Tyr

530 535 540

Gly Gln Thr His Asn Pro Ser Ile Val Thr Phe Leu Leu Asp Pro Ala

545 550 555 560

Glu Asp Ser Lys Val Gly Glu Val His Val Leu Glu Arg Leu Met Glu

565 570 575

Arg Glu His Glu Ala His Ser Leu Leu Gly Asp Ala Ala Ser Leu Met

580 585 590

Gly Lys His Ser Glu Arg Leu Glu Glu Glu Thr Ile Arg Glu Val Leu

595 600 605

Arg Gly Ala Gln Asn Phe Asn Asp Ala Val Ala Asp Pro Ala Glu Val

610 615 620

Leu Glu Asn Pro Ala Gly Leu Asp Asp Ile Asp Trp Leu Leu Ala Gln

625 630 635 640

Ile Ala Gln Ala Asp Ala Lys Ala Glu Thr Glu Ala Glu Ala Glu Thr

645 650 655

Glu Asn Gln Thr Ala Pro Asp Ala Ala Ser Asn Ser Thr Gln His Ala

660 665 670

Gln Arg Arg Leu Tyr Ala Gln Glu Ser Ser Phe Leu Tyr Asp Cys Leu

675 680 685

Leu Glu Gly Phe Asn Asn Val Pro Glu Asp Ser Ile Asn Arg Gly Gly

690 695 700

Val Gly Phe Lys Lys His Asp Asn Asp Ile Val Glu Leu Thr Pro Thr

705 710 715 720

Asp Asp Leu Arg Arg Arg Leu Asp Phe Leu Pro Gln Asp Tyr Val Ala

725 730 735

Ala Arg Lys Val Lys Glu Asp Leu Leu Leu Ala Ser Thr Leu Met Arg

740 745 750

Gly Gln Glu Arg Leu Asn Ala Ala Arg Thr Gly Glu Asp Gly Ser Thr

755 760 765

Trp Pro Ser Ala His Tyr Leu Gly Pro Leu His Pro Val Thr Ser Trp

770 775 780

Ala Ala Asp Arg Ala Leu Ala Thr Met Pro Arg Ser Glu Ile Pro Ala

785 790 795 800

Ala Ser Gly Lys Val Thr Glu Pro Thr Val Leu Leu Met Ser Thr Leu

805 810 815

Ser Asn Arg Arg Gly Gln Ile Val Ser Arg Ser Phe Val Ala Ser Ser

820 825 830

Gly Pro Phe Asp Thr Glu Val Leu Ser Asp Pro Ile Gln Trp Leu His

835 840 845

Ser Ile Gly Leu Asp Glu Thr Ala Ile Asn Pro Gly Thr Ala Ala Leu

850 855 860

Pro Asp Asp Ile Glu Gln Leu Ile Ser Leu Ala Val Gln Ala Ala Arg

865 870 875 880

Gly Glu Ile Arg Pro Leu Met Ile Ala Ala Arg Ala Gln Ala Gln Thr

885 890 895

Arg Val Glu His Trp Ala Lys Arg Ala Glu Ala Trp Asn Asn Lys Arg

900 905 910

Ser Gly Ala Ala Ser Thr Ser Arg Thr Ala Arg Thr Ala Lys Leu Ile

915 920 925

Glu Glu Gln Gln Lys Leu Ser Asn Ala Leu Glu Pro Asp Arg Glu Leu

930 935 940

Ile Arg Pro Leu Ala Val Ile Leu Pro Gln Pro Ala Thr Leu Asn Thr

945 950 955 960

Glu Val

<210>97

<211>1624

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1594)

<223>RXA03793

<400>97

atggaaagaa gctaggcgga aagggcgtta agtacttgcc atttaatcct cagcatcact 60

cggatcagtc ggagatgtcg atgaaaatgc accaggagcc gtg gag agc agc atg 115

Val Glu Ser Ser Met

1 5

gta gaa aac aac gta gca aaa aag acg gtc gct aaa aag acc gca cgc 163

Val Glu Asn Asn Val Ala Lys Lys Thr Val Ala Lys Lys Thr Ala Arg

10 15 20

aag acc gca cgc aaa gca gcc ccg cgc gtg gca acc cca ttg gga gtc 211

Lys Thr Ala Arg Lys Ala Ala Pro Arg Val Ala Thr Pro Leu Gly Val

25 30 35

gca tct gag tct ccc att tcg gcc acc cct gcg cgc agc atc gat gga 259

Ala Ser Glu Ser Pro Ile Ser Ala Thr Pro Ala Arg Ser Ile Asp Gly

40 45 50

acc tca acc cct gtt gaa gct gct gac acc ata gag acc acc gcc cct 307

Thr Ser Thr Pro Val Glu Ala Ala Asp Thr Ile Glu Thr Thr Ala Pro

55 60 65

gca gcg aag gct cct gcg gcc aag gct ccc gct aaa aag gtt gcc aag 355

Ala Ala Lys Ala Pro Ala Ala Lys Ala Pro Ala Lys Lys Val Ala Lys

70 75 80 85

aag aca gct cgc aag gca cct gcg aaa aag act gtc gcc aag aaa gcc 403

Lys Thr Ala Arg Lys Ala Pro Ala Lys Lys Thr Val Ala Lys Lys Ala

90 95 100

aca acc gcc aag gct gca cct gca act gcc aag gac gaa aac gca cct 451

Thr Thr Ala Lys Ala Ala Pro Ala Thr Ala Lys Asp Glu Asn Ala Pro

105 110 115

gtt gat gac gac gag gag aac ctc gct cag gat gaa cag gac ttc gac 499

Val Asp Asp Asp Glu Glu Asn Leu Ala Gln Asp Glu Gln Asp Phe Asp

120 125 130

ggc gat gac ttc gta gac ggc atc gaa gac gaa gaa gat gaa gac ggc 547

Gly Asp Asp Phe Val Asp Gly Ile Glu Asp Glu Glu Asp Glu Asp Gly

135 140 145

gtc gaa gcc ctc ggt gaa gaa agc gaa gac gac gaa gag gac ggc tca 595

Val Glu Ala Leu Gly Glu Glu Ser Glu Asp Asp Glu Glu Asp Gly Ser

150 155 160 165

tcc gtt tgg gat gaa gac gaa tcc gca acc ctg cgt cag gca cgt aaa 643

Ser Val Trp Asp Glu Asp Glu Ser Ala Thr Leu Arg Gln Ala Arg Lys

170 175 180

gat gcc gag ctc acc gct tcc gcc gac tct gtt cgc gct tac ctg aag 691

Asp Ala Glu Leu Thr Ala Ser Ala Asp Ser Val Arg Ala Tyr Leu Lys

185 190 195

caa atc ggt aaa gtt gcc ctg ctg aac gct gaa cag gaa gtc tcc ctg 739

Gln Ile Gly Lys Val Ala Leu Leu Asn Ala Glu Gln Glu Val Ser Leu

200 205 210

gca aag cgc atc gaa gca ggc ctt tac gcc acc cac cgc atg gag gaa 787

Ala Lys Arg Ile Glu Ala Gly Leu Tyr Ala Thr His Arg Met Glu Glu

215 220 225

atg gaa gaa gct ttc gca gcc ggt gac aag gac gcg aaa ctc acc cca 835

Met Glu Glu Ala Phe Ala Ala Gly Asp Lys Asp Ala Lys Leu Thr Pro

230 235 240 245

gcc gtc aag cgt gac ctc cgc gcc atc gct cgt gac ggc cgc aag gcg 883

Ala Val Lys Arg Asp Leu Arg Ala Ile Ala Arg Asp Gly Arg Lys Ala

250 255 260

aaa aac cac ctc ctg gaa gcc aac ctt cgt ctg gtt gtc tcc ctg gca 931

Lys Asn His Leu Leu Glu Ala Asn Leu Arg Leu Val Val Ser Leu Ala

265 270 275

aag cgc tac acc ggc cgt ggc atg gca ttc ctg gac ctc atc cag gaa 979

Lys Arg Tyr Thr Gly Arg Gly Met Ala Phe Leu Asp Leu Ile Gln Glu

280 285 290

ggc aac ctc ggt ctg att cgt gcc gta gag aag ttc gac tac tcc aag 1027

Gly Asn Leu Gly Leu Ile Arg Ala Val Glu Lys Phe Asp Tyr Ser Lys

295 300 305

ggc tac aag ttc tcc acc tac gca acc tgg tgg atc cgt cag gca atc 1075

Gly Tyr Lys Phe Ser Thr Tyr Ala Thr Trp Trp Ile Arg Gln Ala Ile

310 315 320 325

acc cgc gcc atg gcc gac caa gca cga acc atc cgt atc cca gtc cac 1123

Thr Arg Ala Met Ala Asp Gln Ala Arg Thr Ile Arg Ile Pro Val His

330 335 340

atg gtt gaa gtg atc aac aaa ctt ggt cgc atc caa cgt gaa ctc ctt 1171

Met Val Glu Val Ile Asn Lys Leu Gly Arg Ile Gln Arg Glu Leu Leu

345 350 355

cag gaa ctc ggc cgc gaa cca acc cca cag gaa ctg tcc aaa gaa atg 1219

Gln Glu Leu Gly Arg Glu Pro Thr Pro Gln Glu Leu Ser Lys Glu Met

360 365 370

gac atc tcc gag gaa aag gta ctg gaa atc cag cag tac gcc cgc gaa 1267

Asp Ile Ser Glu Glu Lys Val Leu Glu Ile Gln Gln Tyr Ala Arg Glu

375 380 385

cca atc tcc ctg gac caa acc atc ggc gac gaa ggc gac agc cag ctc 1315

Pro Ile Ser Leu Asp Gln Thr Ile Gly Asp Glu Gly Asp Ser Gln Leu

390 395 400 405

ggc gac ttc atc gaa gac tcc gaa gcc gtc gtc gca gtc gac gcc gtc 1363

Gly Asp Phe Ile Glu Asp Ser Glu Ala Val Val Ala Val Asp Ala Val

410 415 420

tca ttc acc ctg ctg caa gac cag cta cag gac gtc cta gag acc ctc 1411

Ser Phe Thr Leu Leu Gln Asp Gln Leu Gln Asp Val Leu Glu Thr Leu

425 430 435

tcc gaa cgt gaa gcc ggc gtg gtt aaa ctc cgc ttc gga ctc acc gac 1459

Ser Glu Arg Glu Ala Gly Val Val Lys Leu Arg Phe Gly Leu Thr Asp

440 445 450

gga atg cca cgc act tta gac gaa atc ggc caa gtt tac ggt gtc acc 1507

Gly Met Pro Arg Thr Leu Asp Glu Ile Gly Gln Val Tyr Gly Val Thr

455 460 465

cgt gag cgc atc cgc cag att gag tcc aag acc atg tct aag ctg cgc 1555

Arg Glu Arg Ile Arg Gln Ile Glu Ser Lys Thr Met Ser Lys Leu Arg

470 475 480 485

cac cca tca cgc tcc cag gtc ctt cgc gac tac ctg gac taaaacccca 1604

His Pro Ser Arg Ser Gln Val Leu Arg Asp Tyr Leu Asp

490 495

gtcgggctca agaccgggcc 1624

<210>98

<211>498

<212>PRT

<213>谷氨酸棒状杆菌

<400>98

Val Glu Ser Ser Met Val Glu Asn Asn Val Ala Lys Lys Thr Val Ala

1 5 10 15

Lys Lys Thr Ala Arg Lys Thr Ala Arg Lys Ala Ala Pro Arg Val Ala

20 25 30

Thr Pro Leu Gly Val Ala Ser Glu Ser Pro Ile Ser Ala Thr Pro Ala

35 40 45

Arg Ser Ile Asp Gly Thr Ser Thr Pro Val Glu Ala Ala Asp Thr Ile

50 55 60

Glu Thr Thr Ala Pro Ala Ala Lys Ala Pro Ala Ala Lys Ala Pro Ala

65 70 75 80

Lys Lys Val Ala Lys Lys Thr Ala Arg Lys Ala Pro Ala Lys Lys Thr

85 90 95

Val Ala Lys Lys Ala Thr Thr Ala Lys Ala Ala Pro Ala Thr Ala Lys

100 105 110

Asp Glu Asn Ala Pro Val Asp Asp Asp Glu Glu Asn Leu Ala Gln Asp

115 120 125

Glu Gln Asp Phe Asp Gly Asp Asp Phe Val Asp Gly Ile Glu Asp Glu

130 135 140

Glu Asp Glu Asp Gly Val Glu Ala Leu Gly Glu Glu Ser Glu Asp Asp

145 150 155 160

Glu Glu Asp Gly Ser Ser Val Trp Asp Glu Asp Glu Ser Ala Thr Leu

165 170 175

Arg Gln Ala Arg Lys Asp Ala Glu Leu Thr Ala Ser Ala Asp Ser Val

180 185 190

Arg Ala Tyr Leu Lys Gln Ile Gly Lys Val Ala Leu Leu Asn Ala Glu

195 200 205

Gln Glu Val Ser Leu Ala Lys Arg Ile Glu Ala Gly Leu Tyr Ala Thr

210 215 220

His Arg Met Glu Glu Met Glu Glu Ala Phe Ala Ala Gly Asp Lys Asp

225 230 235 240

Ala Lys Leu Thr Pro Ala Val Lys Arg Asp Leu Arg Ala Ile Ala Arg

245 250 255

Asp Gly Arg Lys Ala Lys Asn His Leu Leu Glu Ala Asn Leu Arg Leu

260 265 270

Val Val Ser Leu Ala Lys Arg Tyr Thr Gly Arg Gly Met Ala Phe Leu

275 280 285

Asp Leu Ile Gln Glu Gly Asn Leu Gly Leu Ile Arg Ala Val Glu Lys

290 295 300

Phe Asp Tyr Ser Lys Gly Tyr Lys Phe Ser Thr Tyr Ala Thr Trp Trp

305 310 315 320

Ile Arg Gln Ala Ile Thr Arg Ala Met Ala Asp Gln Ala Arg Thr Ile

325 330 335

Arg Ile Pro Val His Met Val Glu Val Ile Asn Lys Leu Gly Arg Ile

340 345 350

Gln Arg Glu Leu Leu Gln Glu Leu Gly Arg Glu Pro Thr Pro Gln Glu

355 360 365

Leu Ser Lys Glu Met Asp Ile Ser Glu Glu Lys Val Leu Glu Ile Gln

370 375 380

Gln Tyr Ala Arg Glu Pro Ile Ser Leu Asp Gln Thr Ile Gly Asp Glu

385 390 395 400

Gly Asp Ser Gln Leu Gly Asp Phe Ile Glu Asp Ser Glu Ala Val Val

405 410 415

Ala Val Asp Ala Val Ser Phe Thr Leu Leu Gln Asp Gln Leu Gln Asp

420 425 430

Val Leu Glu Thr Leu Ser Glu Arg Glu Ala Gly Val Val Lys Leu Arg

435 440 445

Phe Gly Leu Thr Asp Gly Met Pro Arg Thr Leu Asp Glu Ile Gly Gln

450 455 460

Val Tyr Gly Val Thr Arg Glu Arg Ile Arg Gln Ile Glu Ser Lys Thr

465 470 475 480

Met Ser Lys Leu Arg His Pro Ser Arg Ser Gln Val Leu Arg Asp Tyr

485 490 495

Leu Asp

<210>99

<211>1234

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1204)

<223>RXA06048

<400>99

agccgctgct gcagcgtgtc agacattttg gccgtcacct tatcaagttt gtggtgctat 60

cttagagcgc tatatccagc agggtgccca gtttgggtgg atg ttg ttg ctt act 115

Met Leu Leu Leu Thr

1 5

gaa ggc caa gcg ctt aac cct gat ggt cag gga tat cgt cag cgg ttt 163

Glu Gly Gln Ala Leu Asn Pro Asp Gly Gln Gly Tyr Arg Gln Arg Phe

10 15 20

atg aat ggg ttt atc tat tgg cat cct tct act ggt gcg cac gcg gtt 211

Met Asn Gly Phe Ile Tyr Trp His Pro Ser Thr Gly Ala His Ala Val

25 30 35

aat aat tac agt gca caa gtc tgg gag cgt aac ggt tgg gag tct ggg 259

Asn Asn Tyr Ser Ala Gln Val Trp Glu Arg Asn Gly Trp Glu Ser Gly

40 45 50

tgg atg ggg tat ccc act ggt ggt gaa gtc cct gtg tct ggg tct aat 307

Trp Met Gly Tyr Pro Thr Gly Gly Glu Val Pro Val Ser Gly Ser Asn

55 60 65

ccg att gat ggt gag ttg agt ggg tgg gtg caa acc ttc caa ggt ggg 355

Pro Ile Asp Gly Glu Leu Ser Gly Trp Val Gln Thr Phe Gln Gly Gly

70 75 80 85

cga gtg tat cgc agt ccg gta ttg gac ggt ttc cag gtg gcc agt att 403

Arg Val Tyr Arg Ser Pro Val Leu Asp Gly Phe Gln Val Ala Ser Ile

90 95 100

aat ggg ctg atc ttg gat aaa tgg ctt gaa ttg ggt ggt cct gat agt 451

Asn Gly Leu Ile Leu Asp Lys Trp Leu Glu Leu Gly Gly Pro Asp Ser

105 110 115

gac ctt ggt ttt ccc att gcg gat gag gct gtg aca gct gac ggt gtg 499

Asp Leu Gly Phe Pro Ile Ala Asp Glu Ala Val Thr Ala Asp Gly Val

120 125 130

ggc aga ttt tct gtt ttc cag aac gga gtt gtc tac tgg cat ccg caa 547

Gly Arg Phe Ser Val Phe Gln Asn Gly Val Val Tyr Trp His Pro Gln

135 140 145

cac gga gct cac cct ata tta ggg aat ata tac agc atc tgg aga gaa 595

His Gly Ala His Pro Ile Leu Gly Asn Ile Tyr Ser Ile Trp Arg Glu

150 155 160 165

gaa gga gct gag agt ggg gaa ttc ggt tac cct atc ggc gat cca gaa 643

Glu Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro Ile Gly Asp Pro Glu

170 175 180

aag tat aca gaa aac atg gct aat cag gta ttc gaa aaa ggc gaa ctt 691

Lys Tyr Thr Glu Asn Met Ala Asn Gln Val Phe Glu Lys Gly Glu Leu

185 190 195

gca gct aac cta tac ccc aat cct ctt gag gct ttt att gag ttt tta 739

Ala Ala Asn Leu Tyr Pro Asn Pro Leu Glu Ala Phe Ile Glu Phe Leu

200 205 210

ccc ttt gct aat ctt gag gaa gca ata gag tat ttt gag aac gga ttg 787

Pro Phe Ala Asn Leu Glu Glu Ala Ile Glu Tyr Phe Glu Asn Gly Leu

215 220 225

tca aat tct cgt gta gag gcg aat tca ctt aac gcc aag aaa gat tcg 835

Ser Asn Ser Arg Val Glu Ala Asn Ser Leu Asn Ala Lys Lys Asp Ser

230 235 240 245

att caa tgt caa tcg caa tcc gct aac att cat gtg aga acg aag agt 883

Ile Gln Cys Gln Ser Gln Ser Ala Asn Ile His Val Arg Thr Lys Ser

250 255 260

gac gga gtc ggg att agg gtt cca aag att ggg ttt aag gct agg atg 931

Asp Gly Val Gly Ile Arg Val Pro Lys Ile Gly Phe Lys Ala Arg Met

265 270 275

gat tgc gac ctt cct gga act gtc tca gat gta gtg ggg tat gga tgg 979

Asp Cys Asp Leu Pro Gly Thr Val Ser Asp Val Val Gly Tyr Gly Trp

280 285 290

att tac tac gac tat tgg gga cga tgg gct caa gca gca tat gca caa 1027

Ile Tyr Tyr Asp Tyr Trp Gly Arg Trp Ala Gln Ala Ala Tyr Ala Gln

295 300 305

caa ttc ttc ggt aat agg aat tct gtt gtg caa acc aat tta gag gcg 1075

Gln Phe Phe Gly Asn Arg Asn Ser Val Val Gln Thr Asn Leu Glu Ala

310 315 320 325

ggt tgc agc ggg gag aag aat aca tta ttt tgg ggt act tca tat ttt 1123

Gly Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp Gly Thr Ser Tyr Phe

330 335 340

cag gtg act tat gaa ggt cag ccg tat ttc ggt cag tca gca act aac 1171

Gln Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly Gln Ser Ala Thr Asn

345 350 355

tac gct tat ctt ccg tgt acg ata gac cgt agt taacataagg aatggaatag 1224

Tyr Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser

360 365

gagaattgcg 1234

<210>100

<211>368

<212>PRT

<213>谷氨酸棒状杆菌

<400>100

Met Leu Leu Leu Thr Glu Gly Gln Ala Leu Asn Pro Asp Gly Gln Gly

1 5 10 15

Tyr Arg Gln Arg Phe Met Asn Gly Phe Ile Tyr Trp His Pro Ser Thr

20 25 30

Gly Ala His Ala Val Asn Asn Tyr Ser Ala Gln Val Trp Glu Arg Asn

35 40 45

Gly Trp Glu Ser Gly Trp Met Gly Tyr Pro Thr Gly Gly Glu Val Pro

50 55 60

Val Ser Gly Ser Asn Pro Ile Asp Gly Glu Leu Ser Gly Trp Val Gln

65 70 75 80

Thr Phe Gln Gly Gly Arg Val Tyr Arg Ser Pro Val Leu Asp Gly Phe

85 90 95

Gln Val Ala Ser Ile Asn Gly Leu Ile Leu Asp Lys Trp Leu Glu Leu

100 105 110

Gly Gly Pro Asp Ser Asp Leu Gly Phe Pro Ile Ala Asp Glu Ala Val

115 120 125

Thr Ala Asp Gly Val Gly Arg Phe Ser Val Phe Gln Asn Gly Val Val

130 135 140

Tyr Trp His Pro Gln His Gly Ala His Pro Ile Leu Gly Asn Ile Tyr

145 150 155 160

Ser Ile Trp Arg Glu Glu Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro

165 170 175

Ile Gly Asp Pro Glu Lys Tyr Thr Glu Asn Met Ala Asn Gln Val Phe

180 185 190

Glu Lys Gly Glu Leu Ala Ala Asn Leu Tyr Pro Asn Pro Leu Glu Ala

195 200 205

Phe Ile Glu Phe Leu Pro Phe Ala Asn Leu Glu Glu Ala Ile Glu Tyr

210 215 220

Phe Glu Asn Gly Leu Ser Asn Ser Arg Val Glu Ala Asn Ser Leu Asn

225 230 235 240

Ala Lys Lys Asp Ser Ile Gln Cys Gln Ser Gln Ser Ala Asn Ile His

245 250 255

Val Arg Thr Lys Ser Asp Gly Val Gly Ile Arg Val Pro Lys Ile Gly

260 265 270

Phe Lys Ala Arg Met Asp Cys Asp Leu Pro Gly Thr Val Ser Asp Val

275 280 285

Val Gly Tyr Gly Trp Ile Tyr Tyr Asp Tyr Trp Gly Arg Trp Ala Gln

290 295 300

Ala Ala Tyr Ala Gln Gln Phe Phe Gly Asn Arg Asn Ser Val Val Gln

305 310 315 320

Thr Asn Leu Glu Ala Gly Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp

325 330 335

Gly Thr Ser Tyr Phe Gln Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly

340 345 350

Gln Ser Ala Thr Asn Tyr Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser

355 360 365

<210>101

<211>4560

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(1)..(4560)

<223>RXA07005

<400>101

atg gct aaa agc att ctt tcc cga ttc cga cct caa gta gcg gag tgg 48

Met Ala Lys Ser Ile Leu Ser Arg Phe Arg Pro Gln Val Ala Glu Trp

1 5 10 15

ttc cgg gat gtc ttt gca tct ccg acc cct gtt cag gag gga acg tgg 96

Phe Arg Asp Val Phe Ala Ser Pro Thr Pro Val Gln Glu Gly Thr Trp

20 25 30

gag gcg gta tct aag ggg aag aat gcc ctc gtg gtg gcg ccg acc ggt 144

Glu Ala Val Ser Lys Gly Lys Asn Ala Leu Val Val Ala Pro Thr Gly

35 40 45

agt ggt aaa acc ttg gct gcg ttt ttg tgg gcg tta gat tcc ctc act 192

Ser Gly Lys Thr Leu Ala Ala Phe Leu Trp Ala Leu Asp Ser Leu Thr

50 55 60

gaa caa aca ggt caa cag gtt tta gac acg gga aca ccg gtg cct gtt 240

Glu Gln Thr Gly Gln Gln Val Leu Asp Thr Gly Thr Pro Val Pro Val

65 70 75 80

cgt ggt ggg aaa gtg aaa gtg ctc tac att tcc cca ctc aaa gcg ctt 288

Arg Gly Gly Lys Val Lys Val Leu Tyr Ile Ser Pro Leu Lys Ala Leu

85 90 95

ggc gtg gat gta gaa aat aat ctg cgt gca ccg ttg acc ggt att gcg 336

Gly Val Asp Val Glu Asn Asn Leu Arg Ala Pro Leu Thr Gly Ile Ala

100 105 110

agg act gcc tct cgg atg ggt ttg gat gtg ccc aat atc act gtg gcg 384

Arg Thr Ala Ser Arg Met Gly Leu Asp Val Pro Asn Ile Thr Val Ala

115 120 125

gtt cgt tcg ggt gat acg cca tcg gcg gag cgg gcc cgg cag gtg cgt 432

Val Arg Ser Gly Asp Thr Pro Ser Ala Glu Arg Ala Arg Gln Val Arg

130 135 140

aag cct cca gac att ttg atc acc act ccg gag tcg gcg tat ttg atg 480

Lys Pro Pro Asp Ile Leu Ile Thr Thr Pro Glu Ser Ala Tyr Leu Met

145 150 155 160

ttg acc tca aaa gcg ggg gcg acc ctt tcg gat gtt gat gtg gtg atc 528

Leu Thr Ser Lys Ala Gly Ala Thr Leu Ser Asp Val Asp Val Val Ile

165 170 175

atc gat gaa atc cac gcc atg gcc gga acc aaa cgg gga gtg cat ctg 576

Ile Asp Glu Ile His Ala Met Ala Gly Thr Lys Arg Gly Val His Leu

180 185 190

gcg ttg acg ctg gag cgt ttg gaa aag ctc gtg ggg cgg cct gtg cag 624

Ala Leu Thr Leu Glu Arg Leu Glu Lys Leu Val Gly Arg Pro Val Gln

195 200 205

cga gtt ggt ttg tct gca acg gtg cgt cct ttg gaa acg gtg gcg ggt 672

Arg Val Gly Leu Ser Ala Thr Val Arg Pro Leu Glu Thr Val Ala Gly

210 215 220

ttc ttg ggc ggt ggc aga ccc gtt gag att gtg gct cca cct gcg gag 720

Phe Leu Gly Gly Gly Arg Pro Val Glu Ile Val Ala Pro Pro Ala Glu

225 230 235 240

aaa aag tgg gat ctc act gtc act gtg ccg gtg gaa gac atg tcg gat 768

Lys Lys Trp Asp Leu Thr Val Thr Val Pro Val Glu Asp Met Ser Asp

245 250 255

ttg ccg gtt cag gag ccg gga tca act att ggt gaa cta gtc atg gat 816

Leu Pro Val Gln Glu Pro Gly Ser Thr Ile Gly Glu Leu Val Met Asp

260 265 270

gat ccg ttg ggg att act ggc gaa tca gcg ctg cct act caa ggc tcg 864

Asp Pro Leu Gly Ile Thr Gly Glu Ser Ala Leu pro Thr Gln Gly Ser

275 280 285

att tgg cca cac att gag cag cag gtg tac aac cag gtg atg tcg gcg 912

Ile Trp Pro His Ile Glu Gln Gln Val Tyr Asn Gln Val Met Ser Ala

290 295 300

aaa tcg acc atc gtg ttt gta aat tcc agg cgt tcc gcg gag cgt tta 960

Lys Ser Thr Ile Val Phe Val Asn Ser Arg Arg Ser Ala Glu Arg Leu

305 310 315 320

acc agt cgg ttg aat gaa atc tgg gcg atg gaa cac gat ccg gaa tcg 1008

Thr Ser Arg Leu Asn Glu Ile Trp Ala Met Glu His Asp Pro Glu Ser

325 330 335

ctg tcg ccg cag ctg cga aga gat ccg gcg cag att atg tcg tca gcg 1056

Leu Ser Pro Gln Leu Arg Arg Asp Pro Ala Gln Ile Met Ser Ser Ala

340 345 350

gat gtg gca gga aaa gca cca cag gtg atc gca cgt gcg cac cac gga 1104

Asp Val Ala Gly Lys Ala Pro Gln Val Ile Ala Arg Ala His His Gly

355 360 365

tcc gta tcc aaa gat gaa cgt gcc acc acc gaa acc atg ctg aag gaa 1152

Ser Val Ser Lys Asp Glu Arg Ala Thr Thr Glu Thr Met Leu Lys Glu

370 375 380

ggt cgg ttg cgc gca gtt att tcc acc tcc tcg ctg gag ttg ggc att 1200

Gly Arg Leu Arg Ala Val Ile Ser Thr Ser Ser Leu Glu Leu Gly Ile

385 390 395 400

gat atg ggt gcc gtg gac ctg gtg att cag gtg gaa tcg cca ccg tcc 1248

Asp Met Gly Ala Val Asp Leu Val Ile Gln Val Glu Ser Pro Pro Ser

405 410 415

gtg gca agt ggc ctg cag cgc gtg ggg cgt gcg ggg cac acg gtg ggg 1296

Val Ala Ser Gly Leu Gln Arg Val Gly Arg Ala Gly His Thr Val Gly

420 425 430

gcg acg tcg ata ggc tcc ttt tat ccc aag cac cgc tcc gac ttg gtg 1344

Ala Thr Ser Ile Gly Ser Phe Tyr Pro Lys His Arg Ser Asp Leu Val

435 440 445

caa acc gcg gtg acc gtg cag cgg atg aag gaa ggg ctg atc gaa gag 1392

Gln Thr Ala Val Thr Val Gln Arg Met Lys Glu Gly Leu Ile Glu Glu

450 455 460

atc cac gtg ccc aaa aac gcg ctt gat gta ctg gca cag cag acg gtg 1440

Ile His Val Pro Lys Asn Ala Leu Agp Val Leu Ala Gln Gln Thr Val

465 470 475 480

gcg gct gtc tcg att aaa gat gtg cag gtc gat gag tgg tac gag act 1488

Ala Ala Val Ser Ile Lys Asp Val Gln Val Asp Glu Trp Tyr Glu Thr

485 490 495

att cgc aag gcg tat ccg tac cgg gat ttg gcg cgc gaa gtc ttc gat 1536

Ile Arg Lys Ala Tyr Pro Tyr Arg Asp Leu Ala Arg Glu Val Phe Asp

500 505 510

tcc gtc atc gac ctg gtc agc ggt gtg tat ccc tcc aca gat ttt gcc 1584

Ser Val Ile Asp Leu Val Ser Gly Val Tyr Pro Ser Thr Asp Phe Ala

515 520 525

gag ctg aag cca cgt gtg gtg tac gac cgg gtt tca ggc gtg ctg gag 1632

Glu Leu Lys Pro Arg Val Val Tyr Asp Arg Val Ser Gly Val Leu Glu

530 535 540

ggc cgg cca gga tcc caa cgc gta gca gtg acc agt ggc gga aca att 1680

Gly Arg Pro Gly Ser Gln Arg Val Ala Val Thr Ser Gly Gly Thr Ile

545 550 555 560

ccc gat cga gga atg ttc gga gtc ttc ctc gtc ggc gat ggt ccc cgg 1728

Pro Asp Arg Gly Met Phe Gly Val Phe Leu Val Gly Asp Gly Pro Arg

565 570 575

cgc gtc ggc gag ctc gat gag gaa atg gtc tac gaa tcc cgc gtg ggc 1776

Arg Val Gly Glu Leu Asp Glu Glu Met Val Tyr Glu Ser Arg Val Gly

580 585 590

gat gtg ttt acg ctc ggg gcg tcg agt tgg cgg att gaa gag atc acc 1824

Asp Val Phe Thr Leu Gly Ala Ser Ser Trp Arg Ile Glu Glu Ile Thr

595 600 605

cgc gac cag gta ctg gtc act ccc gcg ccg ggt cac acg ggt cgg ctg 1872

Arg Asp Gln Val Leu Val Thr Pro Ala Pro Gly His Thr Gly Arg Leu

610 615 620

cct ttt tgg acg ggc gat gcc gca ggc cgg ccc gct gag ctg ggt aaa 1920

Pro Phe Trp Thr Gly Asp Ala Ala Gly Arg Pro Ala Glu Leu Gly Lys

625 630 635 640

gct tta ggc gct ttt cga cgc tcg acc ctc acc gat cca tcc agc tcc 1968

Ala Leu Gly Ala Phe Arg Arg Ser Thr Leu Thr Asp Pro Ser Ser Ser

645 650 655

ggc ttg gaa ggc tgg gcg cac gac aac ctg atc gcc ttt tta cag gag 2016

Gly Leu Glu Gly Trp Ala His Asp Asn Leu Ile Ala Phe Leu Gln Glu

660 665 670

cag gaa gaa tcc acc ggt gtg ttg ccg gat gag aag acg ttg gtg ttg 2064

Gln Glu Glu Ser Thr Gly Val Leu Pro Asp Glu Lys Thr Leu Val Leu

675 680 685

gag cgt ttc aaa gat gaa cta ggc gac tgg cgc att gtc ctg cac act 2112

Glu Arg Phe Lys Asp Glu Leu Gly Asp Trp Arg Ile Val Leu His Thr

690 695 700

cct tat gga cga gga gta aac gca gca tgg gct ttg gcc gtc ggg gcg 2160

Pro Tyr Gly Arg Gly Val Asn Ala Ala Trp Ala Leu Ala Val Gly Ala

705 710 715 720

aaa atc gct gaa gag acc ggc atg gat gcg caa gcc gtg gca ggt gat 2208

Lys Ile Ala Glu Glu Thr Gly Met Asp Ala Gln Ala Val Ala Gly Asp

725 730 735

gat ggc att gtg ctt cgg ttg ccg gaa ggg gat gaa gat ccc agc gca 2256

Asp Gly Ile Val Leu Arg Leu Pro Glu Gly Asp Glu Asp Pro Ser Ala

740 745 750

gcg ttg ttt atg ttt gag gcg gaa gag atc gaa acg cta gtg aca gag 2304

Ala Leu Phe Met Phe Glu Ala Glu Glu Ile Glu Thr Leu Val Thr Glu

755 760 765

cag gtg ggt aac tct gcg ctg ttt gcc agc agg ttc cgt gaa tgc gcc 2352

Gln Val Gly Asn Ser Ala Leu Phe Ala Ser Arg Phe Arg Glu Cys Ala

770 775 780

gcg agg gcc cta ttg ctg ccg aga cga aac ccc ggc aag cgc gca ccg 2400

Ala Arg Ala Leu Leu Leu Pro Arg Arg Asn Pro Gly Lys Arg Ala Pro

785 790 795 800

ctg tgg cag caa cga caa cga gca gca cag ctt ctt gat gtg gcc aga 2448

Leu Trp Gln Gln Arg Gln Arg Ala Ala Gln Leu Leu Asp Val Ala Arg

805 810 815

aag tac ccg agt ttc ccg atc att ttg gaa aca gtg cgc gaa tgt ctt 2496

Lys Tyr Pro Ser Phe Pro Ile Ile Leu Glu Thr Val Arg Glu cys Leu

820 825 830

caa gat gtt tac gat ctg ccc gct ctg aag aat ctc att gag gat cta 2544

Gln Asp Val Tyr Asp Leu Pro Ala Leu Lys Asn Leu Ile Glu Asp Leu

835 840 845

cag ctg cgg aag gta aga atc gcg gaa gtc acc acc cag cag ccc agt 2592

Gln Leu Arg Lys Val Arg Ile Ala Glu Val Thr Thr Gln Gln Pro Ser

850 855 860

cct ttt gcc tcc gca ttg ctg ttc aat tac acc ggt gca ttc atg tac 2640

Pro Phe Ala Ser Ala Leu Leu Phe Asn Tyr Thr Gly Ala Phe Met Tyr

865 870 875 880

gaa ggc gac agc ccg ctc gca gag aaa cgt gcc gca gcg ttg gcc ctg 2688

Glu Gly Asp Ser Pro Leu Ala Glu Lys Arg Ala Ala Ala Leu Ala Leu

885 890 895

gat ccg gca ctg ttg gcg aaa ttg ctg ggt gag gtg gag ctt cga caa 2736

Asp Pro Ala Leu Leu Ala Lys Leu Leu Gly Glu Val Glu Leu Arg Gln

900 905 910

tta ctg gat ccc gac atc atc gca gaa gtg cac caa caa ttg cgc agg 2784

Leu Leu Asp Pro Asp Ile Ile Ala Glu Val His Gln Gln Leu Arg Arg

915 920 925

caa ggc gat cgt gcg gcg aga aac aat gaa gaa ctc gca gat tct ttg 2832

Gln Gly Asp Arg Ala Ala Arg Asn Asn Glu Glu Leu Ala Asp Ser Leu

930 935 940

agg att tta gga ccg att cct ttg gat gaa ttg ggc gaa cac atc acc 2880

Arg Ile Leu Gly Pro Ile Pro Leu Asp Glu Leu Gly Glu His Ile Thr

945 950 955 960

ttt gaa aac cca gac ctg gag gat cga gca atg act gtt cgg atc aac 2928

Phe Glu Asn Pro Asp Leu Glu Asp Arg Ala Met Thr Val Arg Ile Asn

965 970 975

ggt cgg gaa cat tta gcg cag gtc ttg gat gca cct ttg ctt cga gat 2976

Gly Arg Glu His Leu Ala Gln Val Leu Asp Ala Pro Leu Leu Arg Asp

980 985 990

gcc tta ggt gtt ccc gta ccg cct ggt gtg cct gcg cag gta gaa acc 3024

Ala Leu Gly Val Pro Val Pro Pro Gly Val Pro Ala Gln Val Glu Thr

995 1000 1005

att acg gat gcg ttg gaa cag tta gtc aac agg tgg gtt cgt acc aga 3072

Ile Thr Asp Ala Leu Glu Gln Leu Val Asn Arg Trp Val Arg Thr Arg

1010 1015 1020

ggg cca ttt act gcg aat gat ttg gca gaa gcc ttt gga ctg ggc atc 3120

Gly Pro Phe Thr Ala Asn Asp Leu Ala Glu Ala Phe Gly Leu Gly Ile

1025 1030 1035 1040

gcc acg gcg atc acc gcc ctt caa agc gca cct gtg att gaa ggc cgc 3168

Ala Thr Ala Ile Thr Ala Leu Gln Ser Ala Pro Val Ile Glu Gly Arg

1045 1050 1055

tac cga caa ggc gtg gac gtg cag gaa tac tgt gcg aca gaa gtg ttg 3216

Tyr Arg Gln Gly Val Asp Val Gln Glu Tyr Cys Ala Thr Glu Val Leu

1060 1065 1070

tcg atc ata agg cga cgc agc ctc gca gca gcg agg aaa caa acc agg 3264

Ser Ile Ile Arg Arg Arg Ser Leu Ala Ala Ala Arg Lys Gln Thr Arg

1075 1080 1085

ccg gta tcg caa tca gcc ttt gcg cga ttc ctg ctt gat tgg caa cag 3312

Pro Val Ser Gln Ser Ala Phe Ala Arg Phe Leu Leu Asp Trp Gln Gln

1090 1095 1100

atc gca ccg gtg ggc gcc aca cct gaa ctc cga ggc gtt gat ggc acc 3360

Ile Ala Pro Val Gly Ala Thr Pro Glu Leu Arg Gly Val Asp Gly Thr

1105 1110 1115 1120

tac aca gtc att gaa caa ctc gcc ggt gta cgt ctt ccc gcc agt gcg 3408

Tyr Thr Val Ile Glu Gln Leu Ala Gly Val Arg Leu Pro Ala Ser Ala

1125 1130 1135

tgg gaa gat ctc gtg ttg ccg cgc cgg gtt gcc gac tat tca ccg atc 3456

Trp Glu Asp Leu Val Leu Pro Arg Arg Val Ala Asp Tyr Ser Pro Ile

1140 1145 1150

cat ctc gat gag ctg acc tcc aat ggg gaa gtc ctc atc gtg gga gcg 3504

His Leu Asp Glu Leu Thr Ser Asn Gly Glu Val Leu Ile Val Gly Ala

1155 1160 1165

ggc caa gcc gga agc cgc gat ccg tgg att agc ttg ctg ccc gtg gat 3552

Gly Gln Ala Gly Ser Arg Asp Pro Trp Ile Ser Leu Leu Pro Val Asp

1170 1175 1180

tat gcg gcg cag ttg gtg ggg gag gcg tcg aca agc atg agc cca ttg 3600

Tyr Ala Ala Gln Leu Val Gly Glu Ala Ser Thr Ser Met Ser Pro Leu

1185 1190 1195 1200

cag gac gcc gtg ctt gac cag ctg cgt gcg gga ggc gcc ttc ctg ttt 3648

Gln Asp Ala Val Leu Asp Gln Leu Arg Ala Gly Gly Ala Phe Leu Phe

1205 1210 1215

tct gac att ctc gaa gag aat ttc ggc tac acc aca gcc cag ctg caa 3696

Ser Asp Ile Leu Glu Glu Asn Phe Gly Tyr Thr Thr Ala Gln Leu Gln

1220 1225 1230

gaa gcg atg tgg ggg ctg gtg gaa gca ggc ctg gtc agc cct gat agc 3744

Glu Ala Met Trp Gly Leu Val Glu Ala Gly Leu Val Ser Pro Asp Ser

1235 1240 1245

ttc gcg ccg atc cgc gcg cgc cta gcg tcg gga acc acg gcg cat cgg 3792

Phe Ala Pro Ile Arg Ala Arg Leu Ala Ser Gly Thr Thr Ala His Arg

1250 1255 1260

gcg aaa cgt cga cca gcg aga tcc cgg ctg cgc acc cgc acc agc ttc 3840

Ala Lys Arg Arg Pro Ala Arg Ser Arg Leu Arg Thr Arg Thr Ser Phe

1265 1270 1275 1280

gcg agc gac gtg ccc cca gac atg cgc gga cga tgg acg ctg tcc gtg 3888

Ala Ser Asp Val Pro Pro Asp Met Arg Gly Arg Trp Thr Leu Ser Val

1285 1290 1295

caa ccc gcc gac gcc acc agc cgc tcc gtc gca cac ggc gaa ggc tgg 3936

Gln Pro Ala Asp Ala Thr Ser Arg Ser Val Ala His Gly Glu Gly Trp

1300 1305 1310

ctc gac cgc tac ggc gtg ctc acc cgc ggg agc gtc gtc gcc gaa gac 3984

Leu Asp Arg Tyr Gly Val Leu Thr Arg Gly Ser Val Val Ala Glu Asp

1315 1320 1325

atc gtc gga ggc ttc gcc ctg gcc tac aaa gtg ctc tcc ggc ttc gaa 4032

Ile Val Gly Gly Phe Ala Leu Ala Tyr Lys Val Leu Ser Gly Phe Glu

1330 1335 1340

gaa agc ggc aaa gcg atg cgc ggc tac ttc atc gaa ggg ctc ggc gcc 4080

Glu Ser Gly Lys Ala Met Arg Gly Tyr Phe Ile Glu Gly Leu Gly Ala

1345 1350 1355 1360

gcg caa ttc tcc acg ccc gcc atc atc gac cgc ctc cgc ggc cac gac 4128

Ala Gln Phe Ser Thr Pro Ala Ile Ile Asp Arg Leu Arg Gly His Asp

1365 1370 1375

gat tcc ccc gac gtc gaa ggc tgg ccc tcc ggc gcc acc gac cca gac 4176

Asp Ser Pro Asp Val Glu Gly Trp Pro Ser Gly Ala Thr Asp Pro Asp

1380 1385 1390

gtc tac ctc ata gcc gcc gcc gac ccc gca aac ccc tac ggc gcc gca 4224

Val Tyr Leu Ile Ala Ala Ala Asp Pro Ala Asn Pro Tyr Gly Ala Ala

1395 1400 1405

ctt ccc tgg cct gag cag ggg ccc agc cgc gcc gcc gga gct atg gtc 4272

Leu Pro Trp Pro Glu Gln Gly Pro Ser Arg Ala Ala Gly Ala Met Val

1410 1415 1420

gtg ctt tgc gac gga ctc ctc ctc gcc cac ctc acc cgc ggc ggg cgc 4320

Val Leu Cys Asp Gly Leu Leu Leu Ala His Leu Thr Arg Gly Gly Arg

1425 1430 1435 1440

acc ctc acc gtg ttc tcc gac aat atc ccc aaa atc gcg aca gcc cta 4368

Thr Leu Thr Val Phe Ser Asp Asn Ile Pro Lys Ile Ala Thr Ala Leu

1445 1450 1455

atc aca tac gaa agg ctc acg gta gaa aaa atc aac ggc gac aac gtc 4416

Ile Thr Tyr Glu Arg Leu Thr Val Glu Lys Ile Asn Gly Asp Asn Val

1460 1465 1470

ttc gac tcc cca ctc ctg gaa caa ttc cgc aaa cac ggc gcc acc atc 4464

Phe Asp Ser Pro Leu Leu Glu Gln Phe Arg Lys His Gly Ala Thr Ile

1475 1480 1485

acc ccg aag gga atg cga ttt cga cca cca gtg gca cgg gaa acc ccc 4512

Thr Pro Lys Gly Met Arg Phe Arg Pro Pro Val Ala Arg Glu Thr Pro

1490 1495 1500

tca gat acg ctt ccc acc agg act ttt cgt gga ggc ttc gga cgg cgc 4560

Ser Asp Thr Leu Pro Thr Arg Thr Phe Arg Gly Gly Phe Gly Arg Arg

1505 1510 1515 1520

<210>102

<211>1520

<212>PRT

<213>谷氨酸棒状杆菌

<400>102

Met Ala Lys Ser Ile Leu Ser Arg Phe Arg Pro Gln Val Ala Glu Trp

1 5 10 15

Phe Arg Asp Val Phe Ala Ser Pro Thr Pro Val Gln Glu Gly Thr Trp

20 25 30

Glu Ala Val Ser Lys Gly Lys Asn Ala Leu Val Val Ala Pro Thr Gly

35 40 45

Ser Gly Lys Thr Leu Ala Ala Phe Leu Trp Ala Leu Asp Ser Leu Thr

50 55 60

Glu Gln Thr Gly Gln Gln Val Leu Asp Thr Gly Thr Pro Val Pro Val

65 70 75 80

Arg Gly Gly Lys Val Lys Val Leu Tyr Ile Ser Pro Leu Lys Ala Leu

85 90 95

Gly Val Asp Val Glu Asn Asn Leu Arg Ala Pro Leu Thr Gly Ile Ala

100 105 110

Arg Thr Ala Ser Arg Met Gly Leu Asp Val Pro Asn Ile Thr Val Ala

115 120 125

Val Arg Ser Gly Asp Thr Pro Ser Ala Glu Arg Ala Arg Gln Val Arg

130 135 140

Lys Pro Pro Asp Ile Leu Ile Thr Thr Pro Glu Ser Ala Tyr LeL Met

145 150 155 160

Leu Thr Ser Lys Ala Gly Ala Thr Leu Ser Asp Val Asp Val Val Ile

165 170 175

Ile Asp Glu Ile His Ala Met Ala Gly Thr Lys Arg Gly Val His Leu

180 185 190

Ala Leu Thr Leu Glu Arg Leu Glu Lys Leu Val Gly Arg Pro Val Gln

195 200 205

Arg Val Gly Leu Ser Ala Thr Val Arg Pro Leu Glu Thr Val Ala Gly

210 215 220

Phe Leu Gly Gly Gly Arg Pro Val Glu Ile Val Ala Pro Pro Ala Glu

225 230 235 240

Lys Lys Trp Asp Leu Thr Val Thr Val Pro Val Glu Asp Met Ser Asp

245 250 255

Leu Pro Val Gln Glu Pro Gly Ser Thr Ile Gly Glu Leu Val Met Asp

260 265 270

Asp Pro Leu Gly Ile Thr Gly Glu Ser Ala Leu Pro Thr Gln Gly Ser

275 280 285

Ile Trp Pro His Ile Glu Gln Gln Val Tyr Asn Gln Val Met Ser Ala

290 295 300

Lys Ser Thr Ile Val Phe Val Asn Ser Arg Arg Ser Ala Glu Arg Leu

305 310 315 320

Thr Ser Arg Leu Asn Glu Ile Trp Ala Met Glu His Asp Pro Glu Ser

325 330 335

Leu Ser Pro Gln Leu Arg Arg Asp Pro Ala Gln Ile Met Ser Ser Ala

340 345 350

Asp Val Ala Gly Lys Ala Pro Gln Val Ile Ala Arg Ala His His Gly

355 360 365

Ser Val Ser Lys Asp Glu Arg Ala Thr Thr Glu Thr Met Leu Lys Glu

370 375 380

Gly Arg Leu Arg Ala Val Ile Ser Thr Ser Ser Leu Glu Leu Gly Ile

385 390 395 400

Asp Met Gly Ala Val Asp Leu Val Ile Gln Val Glu Ser Pro Pro Ser

405 410 415

Val Ala Ser Gly Leu Gln Arg Val Gly Arg Ala Gly His Thr Val Gly

420 425 430

Ala Thr Ser Ile Gly Ser Phe Tyr Pro Lys His Arg Ser Asp Leu Val

435 440 445

Gln Thr Ala Val Thr Val Gln Arg Met Lys Glu Gly Leu Ile Glu Glu

450 455 460

Ile His Val Pro Lys Asn Ala Leu Asp Val Leu Ala Gln Gln Thr Val

465 470 475 480

Ala Ala Val Ser Ile Lys Asp Val Gln Val Asp Glu Trp Tyr Glu Thr

485 490 495

Ile Arg Lys Ala Tyr Pro Tyr Arg Asp Leu Ala Arg Glu Val Phe Asp

500 505 510

Ser Val Ile Asp Leu Val Ser Gly Val Tyr Pro Ser Thr Asp Phe Ala

515 520 525

Glu Leu Lys Pro Arg Val Val Tyr Asp Arg Val Ser Gly Val Leu Glu

530 535 540

Gly Arg Pro Gly Ser Gln Arg Val Ala Val Thr Ser Gly Gly Thr Ile

545 550 555 560

Pro Asp Arg Gly Met Phe Gly Val Phe Leu Val Gly Asp Gly Pro Arg

565 570 575

Arg Val Gly Glu Leu Asp Glu Glu Met Val Tyr Glu Ser Arg Val Gly

580 585 590

Asp Val Phe Thr Leu Gly Ala Ser Ser Trp Arg Ile Glu Glu Ile Thr

595 600 605

Arg Asp Gln Val Leu Val Thr Pro Ala Pro Gly His Thr Gly Arg Leu

610 615 620

Pro Phe Trp Thr Gly Asp Ala Ala Gly Arg Pro Ala Glu Leu Gly Lys

625 630 635 640

Ala Leu Gly Ala Phe Arg Arg Ser Thr Leu Thr Asp Pro Ser Ser Ser

645 650 655

Gly Leu Glu Gly Trp Ala His Asp Asn Leu Ile Ala Phe Leu Gln Glu

660 665 670

Gln Glu Glu Ser Thr Gly Val Leu Pro Asp Glu Lys Thr Leu Val Leu

675 680 685

Glu Arg Phe Lys Asp Glu Leu Gly Asp Trp Arg Ile Val Leu His Thr

690 695 700

Pro Tyr Gly Arg Gly Val Asn Ala Ala Trp Ala Leu Ala Val Gly Ala

705 710 715 720

Lys Ile Ala Glu Glu Thr Gly Met Asp Ala Gln Ala Val Ala Gly Asp

725 730 735

Asp Gly Ile Val Leu Arg Leu Pro Glu Gly Asp Glu Asp Pro Ser Ala

740 745 750

Ala Leu Phe Met Phe Glu Ala Glu Glu Ile Glu Thr Leu Val Thr Glu

755 760 765

Gln Val Gly Asn Ser Ala Leu Phe Ala Ser Arg Phe Arg Glu Cys Ala

770 775 780

Ala Arg Ala Leu Leu Leu Pro Arg Arg Asn Pro Gly Lys Arg Ala Pro

785 790 795 800

Leu Trp Gln Gln Arg Gln Arg Ala Ala Gln Leu Leu Asp Val Ala Arg

805 810 815

Lys Tyr Pro Ser Phe Pro Ile Ile Leu Glu Thr Val Arg Glu Cys Leu

820 825 830

Gln Asp Val Tyr Asp Leu Pro Ala Leu Lys Asn Leu Ile Glu Asp Leu

835 840 845

Gln Leu Arg Lys Val Arg Ile Ala Glu Val Thr Thr Gln Gln Pro Ser

850 855 860

Pro Phe Ala Ser Ala Leu Leu Phe Asn Tyr Thr Gly Ala Phe Met Tyr

865 870 875 880

Glu Gly Asp Ser Pro Leu Ala Glu Lys Arg Ala Ala Ala Leu Ala Leu

885 890 895

Asp Pro Ala Leu Leu Ala Lys Leu Leu Gly Glu Val Glu Leu Arg Gln

900 905 910

Leu Leu Asp Pro Asp Ile Ile Ala Glu Val His Gln Gln Leu Arg Arg

915 920 925

Gln Gly Asp Arg Ala Ala Arg Asn Asn Glu Glu Leu Ala Asp Ser Leu

930 935 940

Arg Ile Leu Gly Pro Ile Pro Leu Asp Glu Leu Gly Glu His Ile Thr

945 950 955 960

Phe Glu Asn Pro Asp Leu Glu Asp Arg Ala Met Thr Val Arg Ile Asn

965 970 975

Gly Arg Glu His Leu Ala Gln Val Leu Asp Ala Pro Leu Leu Arg Asp

980 985 990

Ala Leu Gly Val Pro Val Pro Pro Gly Val Pro Ala Gln Val Glu Thr

995 1000 1005

Ile Thr Asp Ala Leu Glu Gln Leu Val Asn Arg Trp Val Arg Thr Arg

1010 1015 1020

Gly Pro Phe Thr Ala Asn Asp Leu Ala Glu Ala Phe Gly Leu Gly Ile

1025 1030 1035 1040

Ala Thr Ala Ile Thr Ala Leu Gln Ser Ala Pro Val Ile Glu Gly Arg

1045 1050 1055

Tyr Arg Gln Gly Val Asp Val Gln Glu Tyr Cys Ala Thr Glu Val Leu

1060 1065 1070

Ser Ile Ile Arg Arg Arg Ser Leu Ala Ala Ala Arg Lys Gln Thr Arg

1075 1080 1085

Pro Val Ser Gln Ser Ala Phe Ala Arg Phe Leu Leu Asp Trp Gln Gln

1090 1095 1100

Ile Ala Pro Val Gly Ala Thr Pro Glu Leu Arg Gly Val Asp Gly Thr

1105 1110 1115 1120

Tyr Thr Val Ile Glu Gln Leu Ala Gly Val Arg Leu Pro Ala Ser Ala

1125 1130 1135

Trp Glu Asp Leu Val Leu Pro Arg Arg Val Ala Asp Tyr Ser Pro Ile

1140 1145 1150

His Leu Asp Glu Leu Thr Ser Asn Gly Glu Val Leu Ile Val Gly Ala

1155 1160 1165

Gly Gln Ala Gly Ser Arg Asp Pro Trp Ile Ser Leu Leu Pro Val Asp

1170 1175 1180

Tyr Ala Ala Gln Leu Val Gly Glu Ala Ser Thr Ser Met Ser Pro Leu

1185 1190 1195 1200

Gln Asp Ala Val Leu Asp Gln Leu Arg Ala Gly Gly Ala Phe Leu Phe

1205 1210 1215

Ser Asp Ile Leu Glu Glu Asn Phe Gly Tyr Thr Thr Ala Gln Leu Gln

1220 1225 1230

Glu Ala Met Trp Gly Leu Val Glu Ala Gly Leu Val Ser Pro Asp Ser

1235 1240 1245

Phe Ala Pro Ile Arg Ala Arg Leu Ala Ser Gly Thr Thr Ala His Arg

1250 1255 1260

Ala Lys Arg Arg Pro Ala Arg Ser Arg Leu Arg Thr Arg Thr Ser Phe

1265 1270 1275 1280

Ala Ser Asp Val Pro Pro Asp Met Arg Gly Arg Trp Thr Leu Ser Val

1285 1290 1295

Gln Pro Ala Asp Ala Thr Ser Arg Ser Val Ala His Gly Glu Gly Trp

1300 1305 1310

Leu Asp Arg Tyr Gly Val Leu Thr Arg Gly Ser Val Val Ala Glu Asp

1315 1320 1325

Ile Val Gly Gly Phe Ala Leu Ala Tyr Lys Val Leu Ser Gly Phe Glu

1330 1335 1340

Glu Ser Gly Lys Ala Met Arg Gly Tyr Phe Ile Glu Gly Leu Gly Ala

1345 1350 1355 1360

Ala Gln Phe Ser Thr Pro Ala Ile Ile Asp Arg Leu Arg Gly His Asp

1365 1370 1375

Asp Ser Pro Asp Val Glu Gly Trp Pro Ser Gly Ala Thr Asp Pro Asp

1380 1385 1390

Val Tyr Leu Ile Ala Ala Ala Asp Pro Ala Asn Pro Tyr Gly Ala Ala

1395 1400 1405

Leu Pro Trp Pro Glu Gln Gly Pro Ser Arg Ala Ala Gly Ala Met Val

1410 1415 1420

Val Leu Cys Asp Gly Leu Leu Leu Ala His Leu Thr Arg Gly Gly Arg

1425 1430 1435 1440

Thr Leu Thr Val Phe Ser Asp Asn Ile Pro Lys Ile Ala Thr Ala Leu

1445 1450 1455

Ile Thr Tyr Glu Arg Leu Thr Val Glu Lys Ile Asn Gly Asp Asn Val

1460 1465 1470

Phe Asp Ser Pro Leu Leu Glu Gln Phe Arg Lys His Gly Ala Thr Ile

1475 1480 1485

Thr Pro Lys Gly Met Arg Phe Arg Pro Pro Val Ala Arg Glu Thr Pro

1490 1495 1500

Ser Asp Thr Leu Pro Thr Arg Thr Phe Arg Gly Gly Phe Gly Arg Arg

1505 1510 1515 1520

<210>103

<211>1251

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(1)..(1251)

<223>RXA07006

<400>103

gtg tcg tct gag aaa gct tca tca aaa tca acc cct gag gca ccg tgg 48

Val Ser Ser Glu Lys Ala Ser Ser Lys Ser Thr Pro Glu Ala Pro Trp

1 5 10 15

cca gtc cgg gaa gta aat act caa gtc aag cag tgg att gaa cgg ctt 96

Pro Val Arg Glu Val Asn Thr Gln Val Lys Gln Trp Ile Glu Arg Leu

20 25 30

ggc cat ttg tgg gtg gag ggc cag ctc gct cag att aat gtg aag ccc 144

Gly His Leu Trp Val Glu Gly Gln Leu Ala Gln Ile Asn Val Lys Pro

35 40 45

aat tgg aag ctg tcg tat ttg acg ctt cgt gat gtg gag caa gaa atg 192

Asn Trp Lys Leu Ser Tyr Leu Thr Leu Arg Asp Val Glu Gln Glu Met

50 55 60

tct gtg cag ctg acc tgc ccg acg gac att atc cgc aat cgc ccc aca 240

Ser Val Gln Leu Thr Cys Pro Thr Asp Ile Ile Arg Asn Arg Pro Thr

65 70 75 80

ccg ctc aag gat ggc gac cgc gtg att gtg tac ggc aag ccc gcg ttt 288

Pro Leu Lys Asp Gly Asp Arg Val Ile Val Tyr Gly Lys Pro Ala Phe

85 90 95

tat gca ggc cgc ggc act ttt tcg ctg tgg gtg act gat atc cgt ccc 336

Tyr Ala Gly Arg Gly Thr Phe Ser Leu Trp Val Thr Asp Ile Arg Pro

100 105 110

gtg ggt att ggt gag ttg ctg gcg cgc att gag gag ctg cgt aaa agg 384

Val Gly Ile Gly Glu Leu Leu Ala Arg Ile Glu Glu Leu Arg Lys Arg

115 120 125

ctt gcc gcg gag ggt ctt ttt gat cca gct cgg aag aag cga ctg cca 432

Leu Ala Ala Glu Gly Leu Phe Asp Pro Ala Arg Lys Lys Arg Leu Pro

130 135 140

ttt ctg ccc aac cgc gtt ggt ttg atc acg gga cgt ggt tca gcg gct 480

Phe Leu Pro Asn Arg Val Gly Leu Ile Thr Gly Arg Gly Ser Ala Ala

145 150 155 160

gag cgc gat gtg ctg agc gtg gct aag gat cgc tgg ccg gaa gtg cag 528

Glu Arg Asp Val Leu Ser Val Ala Lys Asp Arg Trp Pro Glu Val Gln

165 170 175

ttt gag gtg atc aac acg gca gtt cag ggc gct tca gct gtt cct gaa 576

Phe Glu Val Ile Asn Thr Ala Val Gln Gly Ala Ser Ala Val Pro Glu

180 185 190

atc atc gaa gcg ttg cgg gtt tta gat cag gac cct cgc gtg gat gtc 624

Ile Ile Glu Ala Leu Arg Val Leu Asp Gln Asp Pro Arg Val Asp Val

195 200 205

atc atc att gcc cgc ggc ggc ggt tct gtg gag gat ctg ctc ccc ttc 672

Ile Ile Ile Ala Arg Gly Gly Gly Ser Val Glu Asp Leu Leu Pro Phe

210 215 220

tct gag gag gcc ttg cag cgc gca gtc gcg gca gcg cag acg ccc gtg 720

Ser Glu Glu Ala Leu Gln Arg Ala Val Ala Ala Ala Gln Thr Pro Val

225 230 235 240

gtg tcc gcg att ggc cac gaa cca gat acg ccg gtg ttg gac aat gtc 768

Val Ser Ala Ile Gly His Glu Pro Asp Thr Pro Val Leu Asp Asn Val

245 250 255

gcc gac ctt cgc gcg gcg acc ccg acc gat gca gca aag cgc gtg gtg 816

Ala Asp Leu Arg Ala Ala Thr Pro Thr Asp Ala Ala Lys Arg Val Val

260 265 270

cct gat gtg gca gaa gaa cgc atg ttg atc aat cag ctt cgc agt cgt 864

Pro Asp Val Ala Glu Glu Arg Met Leu Ile Asn Gln Leu Arg Ser Arg

275 280 285

agt gcc gcg gcg ttg cgc ggt tgg gtg cag cgc gag cag cag gcg ttg 912

Ser Ala Ala Ala Leu Arg Gly Trp Val Gln Arg Glu Gln Gln Ala Leu

290 295 300

gca gcg att cgc acc agg ccg gtg ctg gct gat ccg atg acc ccg att 960

Ala Ala Ile Arg Thr Arg Pro Val Leu Ala Asp Pro Met Thr Pro Ile

305 310 315 320

aac cgc cga cgt gat gag att gcc cag gct gtg ggc ttg att agg cgc 1008

Asn Arg Arg Arg Asp Glu Ile Ala Gln Ala Val Gly Leu Ile Arg Arg

325 330 335

gat gtc acc cat ctc gtc cgc acc gag caa gca ctg gtg gcg tcg ttg 1056

Asp Val Thr His Leu Val Arg Thr Glu Gln Ala Leu Val Ala Ser Leu

340 345 350

cgc gca cag gtt tcc gcg ctc ggc ccg tcc gca acc ttg gcg cgc ggt 1104

Arg Ala Gln Val Ser Ala Leu Gly Pro Ser Ala Thr Leu Ala Arg Gly

355 360 365

tat tcc gtg gtg cag gtt att cct cgc gac ggc agc gcc ccg gaa gtg 1152

Tyr Ser Val Val Gln Val Ile Pro Arg Asp Gly Ser Ala Pro Glu Val

370 375 380

gtc acc acc atc gag caa tca ccg ccc ggc agc cag ctg cgc atc cgc 1200

Val Thr Thr Ile Glu Gln Ser Pro Pro Gly Ser Gln Leu Arg Ile Arg

385 390 395 400

gtt gcc gac ggc tcc atc act gcg gca tcc atg ggc acc cag caa gca 1248

Val Ala Asp Gly Ser Ile Thr Ala Ala Ser Met Gly Thr Gln Gln Ala

405 410 415

aac 1251

Asn

<210>104

<211>417

<212>PRT

<213>谷氨酸棒状杆菌

<400>104

Val Ser Ser Glu Lys Ala Ser Ser Lys Ser Thr Pro Glu Ala Pro Trp

1 5 10 15

Pro Val Arg Glu Val Asn Thr Gln Val Lys Gln Trp Ile Glu Arg Leu

20 25 30

Gly His Leu Trp Val Glu Gly Gln Leu Ala Gln Ile Asn Val Lys Pro

35 40 45

Asn Trp Lys Leu Ser Tyr Leu Thr Leu Arg Asp Val Glu Gln Glu Met

50 55 60

Ser Val Gln Leu Thr Cys Pro Thr Asp Ile Ile Arg Asn Arg Pro Thr

65 70 75 80

Pro Leu Lys Asp Gly Asp Arg Val Ile Val Tyr Gly Lys Pro Ala Phe

85 90 95

Tyr Ala Gly Arg Gly Thr Phe Ser Leu Trp Val Thr Asp Ile Arg Pro

100 105 110

Val Gly Ile Gly Glu Leu Leu Ala Arg Ile Glu Glu Leu Arg Lys Arg

115 120 125

Leu Ala Ala Glu Gly Leu Phe Asp Pro Ala Arg Lys Lys Arg Leu Pro

130 135 140

Phe Leu Pro Asn Arg Val Gly Leu Ile Thr Gly Arg Gly Ser Ala Ala

145 150 155 160

Glu Arg Asp Val Leu Ser Val Ala Lys Asp Arg Trp Pro Glu Val Gln

165 170 175

Phe Glu Val Ile Asn Thr Ala Val Gln Gly Ala Ser Ala Val Pro Glu

180 185 190

Ile Ile Glu Ala Leu Arg Val Leu Asp Gln Asp Pro Arg Val Asp Val

195 200 205

Ile Ile Ile Ala Arg Gly Gly Gly Ser Val Glu Asp Leu Leu Pro Phe

210 215 220

Ser Glu Glu Ala Leu Gln Arg Ala Val Ala Ala Ala Gln Thr Pro Val

225 230 235 240

Val Ser Ala Ile Gly His Glu Pro Asp Thr Pro Val Leu Asp Asn Val

245 250 255

Ala Asp Leu Arg Ala Ala Thr Pro Thr Asp Ala Ala Lys Arg Val Val

260 265 270

Pro Asp Val Ala Glu Glu Arg Met Leu Ile Asn Gln Leu Arg Ser Arg

275 280 285

Ser Ala Ala Ala Leu Arg Gly Trp Val Gln Arg Glu Gln Gln Ala Leu

290 295 300

Ala Ala Ile Arg Thr Arg Pro Val Leu Ala Asp Pro Met Thr Pro Ile

305 310 315 320

Asn Arg Arg Arg Asp Glu Ile Ala Gln Ala Val Gly Leu Ile Arg Arg

325 330 335

Asp Val Thr His Leu Val Arg Thr Glu Gln Ala Leu Val Ala Ser Leu

340 345 350

Arg Ala Gln Val Ser Ala Leu Gly Pro Ser Ala Thr Leu Ala Arg Gly

355 360 365

Tyr Ser Val Val Gln Val Ile Pro Arg Asp Gly Ser Ala Pro Glu Val

370 375 380

Val Thr Thr Ile Glu Gln Ser Pro Pro Gly Ser Gln Leu Arg Ile Arg

385 390 395 400

Val Ala Asp Gly Ser Ile Thr Ala Ala Ser Met Gly Thr Gln Gln Ala

405 410 415

Asn

Claims

1.分离的核酸分子，其编码具有在每种情况下表1/第2列中所示的氨基酸序列的多肽，其中该核酸分子在表1/第4列所示氨基酸位置处编码与表1/第5列相同行中所示的特定氨基酸不同的蛋白原性氨基酸。

2.权利要求1的分离的核酸分子，其中该核酸分子在表1/第4列中所示的氨基酸位置处编码表1/第6列相同行中所示的氨基酸。

3.一种载体，其含有至少一种如权利要求1的核酸序列。

4.一种宿主细胞，其被至少一种如权利要求3的载体转染。

5.权利要求4的宿主细胞，其中所述核酸分子的表达调节精细化学品自所述细胞的生产。

6.一种生产精细化学品的方法，其包括培养用至少一种权利要求3的载体转染的细胞从而生产该精细化学品。

7.权利要求6的方法，其中精细化学品是氨基酸。

8.权利要求7的方法，其中所述氨基酸是赖氨酸。