KR20050042246A - 유전자 안정성, 유전자 발현 및 폴딩 단백질을 코딩하는유전자 - Google Patents
유전자 안정성, 유전자 발현 및 폴딩 단백질을 코딩하는유전자 Download PDFInfo
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Abstract
본 발명은 신규 핵산 분자, 이들의 유전적으로 개선된 미생물 생산에 있어서의 용도 및 상기 유전적으로 개선된 미생물에 의한 정밀화학물질 (구체적으로, 아미노산) 제조 방법에 관한 것이다.
Description
세포에서 자연발생적으로 일어나는 대사 과정의 일부 산물 및 부산물은 식품, 사료, 화장품 및 제약 산업을 비롯한 다양한 산업 분야에 사용된다. 총체적으로 "정밀화학물질"로 불리는 이들 분자로는 유기산, 단백질생성 및 비단백질생성 아미노산, 뉴클레오티드 및 뉴클레오시드, 지질 및 지방산, 디올, 탄수화물, 방향족 화합물, 비타민, 보조인자 및 효소가 있다. 이들의 생성은 각각의 특정한 경우에 바람직한 분자를 대량으로 생산하고 분비하도록 개발된 세균의 대규모 배양을 통해 가장 효율적으로 수행된다. 이러한 목적에 특히 적합한 유기체는 그람 양성의 비병원성 세균인 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum)이다. 균주 선별을 이용하여, 다양한 바람직한 화합물을 생산하는 다수의 돌연변이 균주가 개발되어 있다. 그러나, 특정 분자의 생산을 위해 개량된 균주의 선별은 시간 소모적이고 어려운 과정이다.
<발명의 개요>
본 발명은 코리네박테리움 글루타미쿰 또는 관련된 세균 종을 확인하거나 분류하는 데 사용될 수 있는 신규 핵산 분자를 제공한다. 씨. 글루타미쿰 (C. glutamicum)은 다수의 정밀화학물질의 대량 생산, 탄화수소의 분해 (예를 들어, 석유 스필) 및 테르페노이드의 산화를 위해 통상적으로 널리 사용되는 그람 양성 호기성 세균이다. 따라서, 본 발명의 핵산 분자는 예를 들어, 발효 공정에 의해 정밀화학물질을 생산하는 데 이용할 수 있는 미생물을 확인하는 데 사용할 수 있다. 비록 씨. 글루타미쿰 자체가 병원성이 아닐지라도, 코리네박테리움 디프테리아 (Corynebacterium diphteria) (디프테리아의 병원체)와 같은, 인간에게 병원성인 다른 코리네박테리움 종과 관련되어 있다. 따라서, 코리네박테리움 종의 존재를 확인하는 능력은 또한 예를 들어, 진단 시험에서 임상적으로 상당히 중요하다. 또한, 상기 핵산 분자는 씨. 글루타미쿰의 게놈 또는 관련 유기체의 게놈을 맵핑하기 위한 기준점으로 제공될 수 있다.
이들 신규 핵산 분자는 본원에 유전자 안정성, 유전자 발현 또는 단백질 분비/단백질 폴딩 (SES) 단백질로 불리는 단백질을 코딩한다. 예를 들어, 이들 SES 단백질은 씨. 글루타미쿰에서의 DNA 복구 또는 재조합, 유전 물질의 이동, 유전자 발현 (즉, 전사 또는 번역과 관련됨), 단백질 폴딩 또는 단백질 분비와 관련된 기능을 수행할 수 있다. 예를 들어, 미국 특허 제4,649,119호 (Sinskey et al.)에 개시된 클로닝 벡터의 이용가능성; 및 코리네박테리움 글루타미쿰 및 관련 브레비박테리움 종(예를 들어, 락토페르멘툼)의 유전자 조작 기술 (Yoshihama et al, J. Bacteriol. 162: 591-597 (1985); Katsumata et al., J. Bacteriol. 159: 306-311 (1984); 및 Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984))을 고려하면, 본 발명의 핵산 분자는 이 유기체가 1종 이상의 정밀화학물질을 보다 효율적으로 생산하게끔 하기 위한 상기 유기체의 유전자 조작에 사용할 수 있다. 정밀화학물질의 향상된 생산성 또는 생산 효율은 직접적으로는 본 발명의 유전자 조작에 의해, 간접적으로는 그러한 유전자 조작에 의해 유래될 수 있다.
본 발명의 SES 단백질을 변화시켜, 그 변화된 단백질을 함유하는 씨. 글루타미쿰 균주로부터의 정밀화학물질 생산의 수율, 생산성 및(또는) 생산 효율에 직접적인 영향을 미칠 수 있는 메카니즘은 여러가지가 있다. 예를 들어, 전사 또는 번역에 직접 관여하는 단백질 (예를 들어, 중합효소 또는 리보솜)을 조작하여 이 단백질의 또는 활성을 증가시킴으로써, 전체적인 세포 전사 또는 번역 (또는 이들 과정의 속도)을 증가시킬 수 있다. 상기 증가된 세포 유전자 발현은 정밀화학물질의 생합성과 관련된 단백질을 포함하며, 그 결과 1종 이상의 관심있는 화합물 생산의 수율, 생산성 또는 효율성을 증가시킬 수 있다. 또한, 씨. 글루타미쿰의 전사/번역 단백질 기구를 조작하여 이 단백질의 조절을 변화시킴으로써 정밀화학물질 생산과 관련된 유전자의 발현을 증가시킬 수 있다. 펩티드 폴딩에 관여하는 다수의 단백질 활성 조절은 세포에서 정확하게 폴딩된 분자의 전체 생산을 증가시킬 수 있으며, 이에 따라 정확하게 기능하는 관심있는 단백질 (예를 들어, 정밀화학물질 생합성 단백질)의 존재 가능성이 증가된다. 또한, 씨. 글루타미쿰으로부터의 분비에 관여하는 단백질을 돌연변이화시켜 이 단백질의 수 또는 활성을 증가시킴으로써 발효 배양액 중 세포로부터의 정밀화학물질 (예를 들어, 효소) 분비를 증가시킬 수 있고, 이에 의해 상기 정밀화학물질을 용이하게 수득할 수 있다.
본 발명 SES 분자의 유전자 조작은 또한 1종 이상의 정밀화학물질 생산을 간접적으로 조절할 수 있다. 예를 들어, 본 발명의 DNA-복구 또는 DNA-재조합 단백질의 수 또는 활성을 증가시킴으로써 DNA 손상을 탐지하고 복구하는 세포 능력을 증가시킬 수 있다. 이것은 돌연변이화된 유전자를 세포 자신의 게놈 내에 유지하는 세포의 기능을 효과적으로 증가시켜, 씨. 글루타미쿰으로 유전자 도입된 트랜스유전자 (transgene) (예를 들어, 정밀화학물질의 생합성을 증가시키는 단백질을 코딩함)가 미생물을 배양하는 동안 손실되지 않고 존재할 가능성을 증가시킬 것이다. 반대로, 1종 이상의 DNA-복구 또는 DNA-재조합 단백질의 수 또는 활성을 감소시켜 유기체의 유전자 불안정성을 증가시킬 수 있다. 이러한 조작은 도입된 돌연변이를 복구시키지 않고도 상기 유기체가 돌연변이화에 의해 변형되는 능력을 개선시킬 것이다. 동일한 결과가 씨. 글루타미쿰에서의 유전자 성분의 전위 또는 재배열에 관여하는 단백질 (예를 들어, 트랜스포손)의 경우에도 나타날 것이다. 단백질을 돌연변이화하여 이들 단백질의 수 또는 활성을 증가시키거나 감소시킴으로써, 동시에 미생물의 유전자 안정성을 증가시키거나 감소시킬 수 있다. 이것은 씨. 글루타미쿰으로 다른 돌연변이가 도입될 가능성과 도입된 돌연변이가 유지될 가능성에 중대한 영향을 미친다. 이와 마찬가지로 트랜스포손은 트랜스포손 돌연변이화에 의해 용이하에 수행될 수 있는 원치않는 유전자 (예를 들어, 관심있는 정밀화학물질의 분해와 관련된 유전자)의 붕괴 뿐만 아니라 씨. 글루타미쿰의 돌연변이화 및 관심있는 유전자 (예를 들어, 정밀화학물질 생합성 유전자)의 복제를 가능하게 하는 적합한 메카니즘을 제공한다.
특정 환경 조건에 대한 반응에서 전사 또는 번역 조절에 관여하는 1종 이상의 단백질 (예를 들어, 시그마 인자)을 조작함으로써 세포의 대규모 발효 배양시 나타나는 바람직하지 못한 환경 조건하에 세포에서 단백질 생산이 지연되거나 중단되는 것을 방지한다. 이것은 유전자 발현을 증가시킬 것이며, 또한 상기 조건하에서 정밀화학물질의 생합성을 증가시킬 수 있다. 분비 시스템과 관련된 단백질의 돌연변이화는 분비 속도를 조절할 수 있도록 한다. 이들 분비 단백질 중 상당수가 세포 생존율에 중요한 기능을 갖는다 (예를 들어, 세포 표면 프로테아제 또는 세포 표면 수용체). 이들 단백질이 세포 밖으로 보다 용이하게 수송되도록 분비 경로를 변화시켜 전체적인 세포의 생존률을 증가시킬 수 있기 때문에, 대규모로 배양하는 동안 보다 많은 씨. 글루타미쿰 세포에서 정밀화학물질을 생산할 수 있게 된다. 또한, 분비 장치 (예를 들어, sec 시스템)가 내재성 막 단백질 (예를 들어, 포어, 채널 또는 수송자)이 막으로 삽입되는 과정에도 관여한다는 것이 공지되어 있다. 따라서, 씨. 글루타미쿰으로부터의 단백질 분비에 관여하는 단백질의 활성을 조절하여 폐기물을 분비하거나 필요한 대사 산물을 들여오는 세포 능력에 영향을 미칠 수 있다. 상기 분비 단백질의 활성이 증가하면 정밀화학물질을 생산하는 세포 능력도 증가할 수 있다. 상기 분비 단백질의 활성이 감소하면 관심있는 화합물을 과다생산하도록 하는 영양소가 충분히 존재하지 않거나 폐기물이 관심있는 화합물의 생합성을 방해할 수 있다.
본 발명은 본원에서 SES 단백질이라 불리우는 단백질을 코딩하며, 예를 들어 코리네박테리움 글루타미쿰에서 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비에 관여할 수 있는 핵산 분자를 제공한다. SES 단백질을 코딩하는 핵산 분자를 본원에서 SES 핵산 분자로 언급된다. 바람직한 실시양태에서, SES 단백질은 씨. 글루타미쿰에서의 유전자 안정성의 증진 또는 감소, 상기 미생물에서의 유전자 발현 (예를 들어, 전사 또는 번역) 또는 단백질 폴딩, 또는 씨. 글루타미쿰으로부터의 단백질 분비에 관여한다. 상기 단백질의 예는 표 1에 나열된 유전자들에 의해 코딩되는 단백질들이다.
따라서, 본 발명의 한 측면은 SES 단백질 또는 이들의 생물학적 활성 부분을 코딩하는 뉴클레오티드 서열을 포함하는 단리된 핵산 분자 (예를 들어, cDNA) 뿐만 아니라 SES 코딩 핵산 (예를 들어, DNA 또는 mRNA)의 검출 또는 증폭을 위한 프라이머 또는 혼성화 프로브로서 적합한 핵산 단편에 관한 것이다. 특히 바람직한 실시양태에서, 단리된 핵산 분자는 부록 A에 나열된 임의의 뉴클레오티드 서열 또는 이들 뉴클레오티드 서열 중 하나의 코딩 영역 또는 이들의 상보체를 포함한다. 다른 바람직한 실시양태에서, 단리된 핵산 분자는 부록 B에 나열된 아미노산 서열 중 하나를 코딩한다. 또한, 본 발명의 바람직한 SES 단백질은 본원에 기재된 SES 활성 중 적어도 하나를 갖는 것이 바람직하다.
부록 A는 하기 표 1에 기재된, 관련된 위치에서의 서열 변형과 함께 나열한 서열목록의 핵산 서열을 정의한다.
부록 B는 하기 표 1에 기재된, 관련된 위치에서의 서열 변형과 함께 나열한 서열목록의 핵산 서열을 정의한다.
추가의 실시양태에서, 단리된 핵산 분자는 그 길이가 15개의 뉴클레오티드 이상이고 부록 A의 뉴클레오티드 서열을 포함하는 핵산 분자와 엄격한 조건 하에 혼성화된다. 단리된 핵산 분자는 자연 발생 핵산 분자에 상응하는 것이 바람직하다. 단리된 핵산은 자연 발생 씨. 글루타미쿰 SES 단백질 또는 그의 생물학적 활성 부분을 코딩하는 것이 보다 바람직하다.
본 발명의 추가 측면은 본 발명의 핵산 분자를 함유하는 벡터, 예를 들어 재조합 발현 벡터, 및 이러한 백터가 도입된 숙주 세포에 관한 것이다. 한 실시양태에서, SES 단백질은 적합한 배지에서 배양한 상기 숙주 세포를 사용하여 생산된다. 그 후, SES 단백질을 배지 또는 숙주 세포로부터 단리할 수 있다.
본 발명의 추가 측면은 SES 유전자가 도입되거나 변이된 유전자 변이 미생물에 관한 것이다. 한 실시양태에서, 이들 미생물의 게놈은 하나 이상의 돌연변이 SES 서열을 코딩하는 본 발명의 핵산 분자를 트랜스 유전자로서 도입하여 변이시킨다. 다른 실시양태에서, 상기 미생물 게놈 내의 내생성 SES 유전자는 변이된 SES 유전자와의 상동성 재조합에 의해 변이, 예를 들어 기능적으로 붕괴된다. 바람직한 실시양태에서, 상기 미생물은 코리네박테리움 또는 브레비박테리움 (Brevibacterium) 속, 특히 바람직하게는 코리네박테리움 글루타미쿰에 속한다. 바람직한 실시양태에서, 상기 미생물은 아미노산, 특히 바람직하게는 리신과 같은 관심있는 화합물의 생산에도 사용된다.
본 발명의 추가 측면은 단리된 SES 단백질 또는 그의 부분, 예를 들어, 그의 생물학적 활성 부분에 관한 것이다. 바람직한 실시양태에서, 단리된 SES 단백질 또는 그의 부분은 코리네박테리움 글루타미쿰에서 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여할 수 있다. 또다른 바람직한 실시양태에서, 단리된 SES 단백질 또는 그의 부분은, 예를 들어 코리네박테리움 글루타미쿰에서 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여하는 능력을 보유한 단백질 또는 그의 부분에 대한 부록 B의 아미노산 서열과 충분한 상동성이 있다.
또다른 바람직한 실시양태는 부록 A에 기재된 핵산 분자 중 하나 이상을 갖는 숙주 세포이다. 상기 숙주 세포는 당업자에게 공지된 다양한 방법에 의해 생산될 수 있다. 예를 들어, 이들 숙주 세포는 여러 개의 본 발명의 핵산 분자를 운반하는 벡터에 의해 형질전환될 수 있다. 그러나, 본 발명의 핵산 분자 하나를 숙주 세포에 도입하기 위한 벡터를 사용하는 것도 가능하기 때문에, 대다수의 벡터를 동시에 또는 순차적으로 사용할 수 있다. 따라서, 수많은 (수백개 이하) 본 발명의 핵산 서열을 운반하는 숙주 세포를 제조할 수 있다. 이와 같은 축적은 숙주 세포의 정밀화학물질 생산률에 상승 효과를 미칠 수 있다.
또한, 본 발명은 SES 단백질의 단리된 제제를 제공한다. 바람직한 실시양태에서, SES 단백질은 부록 B의 아미노산 서열을 포함한다. 추가의 바람직한 실시양태에서, 본 발명은 부록 B의 전체 아미노산 서열 (부록 A의 오픈 리딩 프레임에 의해 코딩됨)과 실질적으로 상동성이 있는 단리된 전장 단백질에 관한 것이다.
SES 폴리펩티드 또는 이들의 생물학적 활성 부분를 비-SES 폴리펩티드와 기능적으로 연결하여 융합 단백질을 형성할 수 있다. 바람직한 실시양태에서, 이 융합 단백질의 활성은 SES 단백질만의 활성과는 다르다. 다른 바람직한 실시양태에서, 이 융합 단백질은 코리네박테리움 글루타미쿰에서 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여한다. 특히 바람직한 실시양태에서, 이 융합 단백질을 숙주 세포로 통합시켜 이 세포들로부터 관심있는 화합물이 생성되는 것을 조절한다.
본 발명의 추가 측면은 정밀화학물질의 제조 방법에 관한 것이다. 이 방법은 본 발명의 SES 핵산 분자를 발현하는 세포를 배양하여 정밀화학물질을 생산하는 방법을 제공한다. 바람직한 실시양태에서, 이 방법은 SES 핵산을 발현하는 벡터로 세포를 형질감염시켜 상기 벡터를 함유하는 세포를 수득하는 단계도 포함한다. 추가의 바람직한 실시양태에서, 이 방법은 배양물로부터 정밀화학물질을 회수하는 단계도 포함한다. 특히 바람직한 실시양태에서, 세포는 코리네박테리움 또는 브레비박테리움 속에 속한다.
본 발명의 추가 측면은 미생물로부터의 분자 생산을 조절하는 방법에 관한 것이다. 이 방법은 SES 단백질 활성 또는 SES 핵산 발현을 조절하는 물질과 세포를 접촉시켜, 상기 물질의 부재 하에 세포-관련 활성이 동일 활성과 비교하였을 때 변화되는 단계를 포함한다. 바람직한 실시양태에서, 씨. 글루타미쿰은 유전자 안정성, 유전자 발현, 단백질 폴딩 또는 단백질 분비와 관련된 세포 과정 중 하나 이상을 조절하여 이 미생물에 의한 관심있는 정밀화학물질 생산의 수율, 생산성 또는 생산 효율을 개선시킨다. SES 단백질 활성을 조절하는 물질은 SES 단백질 활성 또는 SES 핵산 발현을 자극하는 물질일 수 있다. SES 단백질 활성 또는 SES 핵산 발현을 자극하는 물질의 예로는 소분자, 활성 SES 단백질, 및 세포에 도입된 SES 단백질을 코딩하는 핵산이 있다. SES 활성 또는 발현을 억제하는 물질의 예로는 소분자 및 안티센스 SES 핵산 분자가 있다.
본 발명의 추가 측면은 별개의 플라스미드 상에 유지되어 있거나 숙주 세포의 게놈 내로 통합되어 있도록 야생형 또는 SES 돌연변이 유전자를 세포에 도입하는 것을 포함하는, 세포로부터의 관심있는 화합물의 수율을 조절하는 방법에 관한 것이다. 게놈 내로 통합되면 (이러한 통합은 무작위적일 수 있거나 천연 유전자가 도입된 카피로 대체되도록 하는 상동성 재조합에 의해 일어날 수 있음) 세포로부터의 관심있는 화합물의 생산이 조절된다. 바람직한 실시양태에서, 상기 수율은 증가한다. 추가의 바람직한 실시양태에서, 화학제품은 정밀화학물질이며, 특히 바람직한 실시양태에서는 아미노산이다. 특히 바람직한 실시양태에서, 상기 아미노산은 L-리신이다.
본 발명은 코리네박테리움 글루타미쿰에서 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비에 관여하는 SES 핵산 및 SES 단백질 분자를 제공한다. 본 발명의 분자는 씨. 글루타미쿰과 같은 미생물로부터 직접적으로 정밀화학물질의 생산을 조절 (예를 들어, 정밀화학물질 (예를 들어, 효소)의 분비와 관련된 단백질을 과다발현하거나 그 활성을 최적화시켜 변형된 씨. 글루타미쿰 세포로부터 정밀화학물질의 수율, 생산성 및(또는) 생산 효율에 직접적인 영향을 미침)하는데 이용하거나, 또는 간접적인 영향이지만 관심있는 화합물의 수율, 생산성 및(또는) 생산 효율을 증가시키는 방식으로 정밀화학물질의 생산을 조절 (예를 들어, 씨. 글루타미쿰 DNA-복구 단백질의 활성 또는 카피수를 조절하여 도입된 돌연변이를 유지하는 미생물의 능력을 변화시키며, 이는 후에 상기 균주로부터 1종 이상의 정밀화학물질을 생산하는 데 영향을 미침)하는 데 이용할 수 있다. 본 발명의 측면은 하기에 추가로 예시되어 있다.
Ⅰ. 정밀화학물질
용어 "정밀화학물질"은 당업계에 인지되어 있고, 제약, 농업 및 화장품 산업과 같은, 그러나 이에 제한되지 않는 다양한 산업 분야에 이용되는 유기체에 의해 생산되는 분자를 포함한다. 이러한 화합물로는 타르타르산, 이타콘산 및 디아미노피멜산과 같은 유기산, 단백질생성 및 비단백질생성 아미노산, 퓨린 및 피리미딘 염기, 뉴클레오시드, 및 뉴클레오티드 (예를 들어, 문헌 [Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim] 및 이 문헌에 포함된 참고문헌에 기재되어 있음), 지질, 포화 및 불포화 지방산 (예를 들어, 아라키돈산), 디올 (예를 들어, 프로판디올 및 부탄디올), 탄수화물 (예를 들어, 히알루론산 및 트레할로스), 방향족 화합물 (예를 들어, 방향족 아민, 바닐린 및 인디고), 비타민 및 보조인자 (문헌 [Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim] 및 이 문헌에 포함된 참고문헌; 및 [Ong, A. S., Niki, E. ＆ Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3,1994 at Penang, Malaysia, AOCS Press, (1995)]에 기재되어 있음), 효소, 및 문헌 [Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086] 및 이 문헌에 포함된 참고문헌에 기재된 다른 모든 화학물질이 있다. 특정 정밀화학물질의 대사 및 용도는 아래에서 추가로 설명될 것이다.
A. 아미노산 대사 및 용도
아미노산은 모든 단백질의 기본 구조 단위를 구성하므로 모든 유기체에서 정상적인 세포 기능에 필수적이다. 용어 "아미노산"은 당업계에 인지되어 있다. 아미노산 중 단백질생성 아미노산은 20종이고 이들이 펩티드 결합에 의해 결합되어 있는 단백질에 대한 구조 단위로 작용하는 반면, 비단백질생성 아미노산 (수백 종이 공지되어 있음)은 통상적으로는 단백질에서 발견되지 않는다 (문헌 [Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)] 참조). 비록 L-아미노산이 통상적으로 자연 발생 단백질에서 발견되는 유일한 형태일지라도 아미노산은 D-광학 또는 L-광학 구조로 존재할 수 있다. 20종의 단백질생성 아미노산 각각의 생합성 및 분해 경로의 특징은 원핵세포 및 진핵세포 둘다에서 잘 규명되어 있다 (예를 들어, 문헌 [Stryer, L. Biochemistry, 3rd edition, pages 578-590 (1988)] 참조). 생합성의 복잡성으로 인해 통상적으로 식이 섭취해야 하기 때문에 "필수" 아미노산이라고 불리는 아미노산 (히스티딘, 이소루이신, 루이신, 리신, 메티오닌, 페닐알라닌, 트레오닌, 트립토판 및 발린)은 간단한 생합성 경로에 의해 나머지 11종의 "비필수" 아미노산 (알라닌, 아르기닌, 아스파라긴, 아스파테이트, 시스테인, 글루타메이트, 글루타민, 글리신, 프롤린, 세린 및 티로신)으로 전환된다. 고등동물은 필수 아미노산 중 일부를 합성할 수 있지만, 상기 필수 아미노산은 음식으로 섭취되어야 정상적인 단백질 합성이 일어난다.
단백질 생합성에서의 아미노산의 기능 이외에, 이들 아미노산은 그 자체가 흥미로운 화학물질이고, 다수의 아미노산이 식품, 동물 사료, 화학물질, 화장품, 농업 및 제약 산업에 다양하게 사용될 수 있는 것으로 밝혀져 있다. 리신은 인간 뿐만 아니라 가금 및 돼지와 같은 단위 (monogastric) 동물의 영양에 있어서도 중요한 아미노산이다. 글루타메이트는 향미제 첨가물 (모노-소듐 글루타메이트, MSG)로서 가장 흔히 사용되고, 아스파테이트, 페닐알라닌, 글리신 및 시트테인도 식품 산업 전반에 걸쳐 광범위하게 사용된다. 글리신, L-메티오닌 및 트립토판은 모두 제약 산업에 사용된다. 글루타민, 발린, 루이신, 이소루이신, 히스티딘, 아르기닌, 프롤린, 세린 및 알라닌은 제약 및 화장품 산업에 사용된다. 트레오닌, 트립토판 및 D/L-메티오닌은 널리 사용되는 사료 첨가제이다 (Leuchtenberger, W. (1996) Amino acids technical production and use, p. 466-502 in Rehm et al. (editors) Biotechnology vol. 6, chapter 14a, VCH: Weinheim). 또한, 이들 아미노산은 합성 아미노산 및 단백질, 예를 들어, N-아세틸시스테인, S-카르복시메틸-L-시스테인, (S)-5-히드록시트립토판, 및 문헌 [Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985]에 기재된 다른 물질의 합성을 위한 전구체로 적합하다는 것이 밝혀졌다.
이들 천연 아미노산을 생산할 수 있는 유기체, 예를 들어, 세균에서의 이들 천연 아미노산의 생합성의 특징은 잘 규명되어 있다 (세균 아미노산 생합성 및 그의 조절에 대한 전반적인 내용은 문헌 [Umbarger, H. E. (1978) Ann. Rev. Biochem. 47: 533-606]을 참조함). 글루타메이트는 시트르산 사이클의 중간 산물인 α-케토글루타레이트의 환원성 아민화에 의해 합성된다. 그 다음에 글루타민, 프롤린 및 아르기닌 각각이 글루타메이트로부터 생성된다. 세린의 생합성은 3-포스포글리세레이트 (해당작용의 중간 산물)로 출발하며, 산화, 트랜스아민화 및 가수분해 단계 후 세린을 생성하는 3 단계 과정으로 수행된다. 시스테인 및 글리신은 각각 세린으로부터 생성되는데, 시스테인은 호모시스테인과 세린의 축합에 의해 생성되며, 글리신은 세린 트랜스히드록시메틸라제에 의해 촉매되는 반응에서 측쇄 β-탄소 원자가 테트라히드로폴레이트로 전달되어 생성된다. 페닐알라닌 및 티로신은 프레페네이트의 합성 후 최종 두 단계에서만 분지되는 9 단계 생합성 경로에서 해당작용 및 펜토스 포스페이트 경로 전구체인 에리쓰로스 4-포스페이트 및 포스포에놀피루베이트로부터 합성된다. 트립토판도 이들 두 초기 분자로부터 합성되지만, 그의 합성은 11 단계 경로이다. 티로신은 페닐알라닌 히드록실라제에 의해 촉매되는 반응에서 페닐알라닌으로부터 합성될 수도 있다. 알라닌, 발린 및 루이신 각각은 해당작용의 최종 생성물인 피루베이트로부터 유래된 생합성 산물이다. 아스파테이트는 시트르산 사이클의 중간 산물인 옥살로아세테이트로부터 형성된다. 아스파라긴, 메티오닌, 트레오닌 및 리신 각각은 아스파테이트의 전환에 의해 생성된다. 이소루이신은 트레오닌으로부터 형성된다. 복잡한 9 단계 경로에서 활성화된 당인 5-포스포리보실-1-피로포스페이트로부터 히스티딘이 생성된다.
단백질 생합성에 필요한 양을 초과하는 아미노산은 저장될 수 없고, 그 대신에 분해되어 세포의 주요 대사 경로에 대한 중간 산물로 제공된다 (전반적인 내용은 문헌 [Stryer, L. Biochemistry 3rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)]을 참조함). 세포가 불필요한 아미노산을 적합한 대사 중간 산물로 전환시킬 수 있다 하더라도, 아미노산 생성은 이들 아미노산을 합성하는 데 필요한 에너지, 전구체 분자 및 효소를 고려할 때 손실이 큰 합성이다. 따라서, 특정한 아미노산의 존재가 그 자신의 생성을 서서히 또는 완전히 중지시키는 피드백 억제에 의해 아미노산의 생합성이 조절된다는 것은 놀라운 일이 아니다 (아미노산 생합성 경로의 피드백 메카니즘에 대한 전반적인 내용은 문헌 [Stryer, L. Biochemistry 3rd ed. Ch. 24 "Biosynthesis of Amino Acids and Heme" p. 575-600 (1988)]을 참조함). 따라서, 특정 아미노산의 산출량은 세포에 존재하는 아미노산의 양에 의해 제한된다.
B. 비타민, 보조인자 및 영양제의 대사 및 용도
비타민, 보조인자 및 영양제는 또다른 분자 군을 포함한다. 이들은 세균과 같은 다른 유기체에 의해 용이하게 합성된다 하더라도 고등동물은 이들을 합성하는 능력을 상실하였기 때문에 섭취해야만 한다. 이들 분자는 그 자체가 생활성 물질이거나, 다수의 대사 경로에서 전자 캐리어 또는 중간 산물로 작용할 수 있는 생활성 물질의 전구체이다. 이들의 영양 가치 이외에, 이들 화합물은 색소, 항산화제 및 촉매로서, 또는 다른 과정의 보조제로서 상당한 산업적 가치를 갖는다 (이들 화합물의 구조, 활성 및 산업적인 용도에 대한 전반적인 내용은 예를 들어, 문헌 [Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996]을 참조함). 용어 "비타민"은 당업계에 인지되어 있고, 유기체의 정상적인 기능을 위해서는 필요하지만 유기체가 스스로 합성할 수 없는 영양분을 포함한다. 비타민 군은 보조인자 및 영양제 화합물도 포함할 수 있다. 용어 "보조인자"는 정상적인 효소 활성이 일어나는 데 필요한 비단백질생성 화합물을 포함한다. 이러한 화합물은 유기성 또는 무기성일 수 있고, 본 발명의 보조인자 분자는 유기성인 것이 바람직하다. 용어 "영양제"는 식물 및 동물, 특히 인간에서 건강을 증진시키는 식이성 보충물을 포함한다. 이러한 분자의 예로는 비타민, 항산화제 및 일부 지질 (예를 들어, 다불포화 지방산)이 있다.
이들을 생성할 수 있는 유기체, 예를 들어, 세균에서 일어나는 이들 분자의 생합성의 특징은 이해하기 쉽게 규명되어 있다 (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley ＆ Sons; Ong, A. S., Niki, E. ＆ Packer, L. (1995) "Nutrition, Lipids, Health, and Disease "Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research-Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).
티아민 (비타민 B1)은 피리미딘과 티아졸 잔기의 화학적 커플링에 의해 생성된다. 리보플라빈 (비타민 B2)는 구아노신-5'-트리포스페이트 (GTP) 및 리보스-5'-포스페이트로부터 합성된다. 그 다음, 리보플라빈은 플라빈 모노뉴클레오티드 (FMN) 및 플라빈 아데닌 디뉴클레오티드 (FAD)의 합성에 사용된다. 총칭하여 "비타민 B6"로 불리는 화합물 족 (예를 들어, 피리독신, 피리독사민, 피리독사-5'-포스페이트 및 시판되는 피리독신 히드로클로라이드)은 모두 통상적인 구조 단위인 5-히드록시-6-메틸피리딘의 유도체이다. 판토테네이트 (판토텐산, (R)-(+)-N-(2,4-디히드록시-3,3-디메틸-1-옥소부틸)-β-알라닌)은 화학적인 합성 또는 발효에 의해 생성될 수 있다. 판토테네이트의 생합성의 최종 단계는 β-알라닌과 판토산의 ATP-유도 축합으로 구성된다. 판토산으로 전환하는 생합성 단계, β-알라닌으로 전환하는 생합성 단계 및 판토텐산으로의 축합에 관여하는 효소는 공지되어 있다. 판토테네이트의 대사 활성 형태는 조효소 A인데, 이 조효소 A의 생합성은 5 단계 효소 반응으로 진행된다. 판토테네이트, 피리독살-5'-포스페이트, 시스테인 및 ATP는 조효소 A의 전구체이다. 이들 효소는 판토테네이트의 형성을 촉매할 뿐만 아니라 (R)-판토산, (R)-판토락톤, (R)-판테놀 (프로비타민 B5), 판테테인 (및 그의 유도체) 및 조효소 A의 생성도 촉매한다.
미생물에서 전구체 분자인 피멜로일-CoA로부터 바이오틴이 생합성되는 것은 자세히 연구되어 있고 관련된 여러가지 유전자가 확인되어 있다. 상응하는 다수의 단백질이 Fe-클러스터 합성에도 관여하는 것으로 밝혀져 있고 이들 단백질은 nifS 단백질 군에 속한다. 리포산은 옥탄산으로부터 유도되고, 에너지 대사에서 조효소로 작용하는데, 상기 리포산은 에너지 대사에서 피루베이트 데히드로게나제 복합체 및 α-케토글루타레이트 데히드로게나제 복합체의 일부를 구성한다. 폴레이트는 모두 엽산으로부터 유도된 물질의 군이고, 엽산은 L-글루탐산, p-아미노벤조산 및 6-메틸프테린으로부터 유도된다. 생체형질전환의 대사 중간 산물인 구아노신-5'-트리포스페이트 (GTP), L-글루탐산 및 p-아미노-벤조산으로부터 출발하는, 엽산 및 그의 유도체의 생합성은 일부 미생물에서 상세히 연구되어 있다.
코리노이드 (예를 들어, 코발라민 및 특히 비타민 B12) 및 포피린은 테트라피롤 고리계에 의해 특징지워지는 화학물질의 군에 속한다. 비타민 B12의 생합성은 매우 복잡하여 아직까지 그 특징이 완전히 규명되어 있지는 않지만 관련된 다수의 효소 및 기질이 현재 공지되어 있다. 니코틴산 (니코티네이트) 및 니코틴아미드는 "니아신"으로도 불리는 피리딘 유도체이다. 니아신은 중요한 조효소인 NAD (니코틴아미드 아데닌 디뉴클레오티드), NADP (니코틴아미드 아데닌 디뉴클레오티드 포스페이트) 및 그들의 환원형의 전구체이다.
이들 화합물의 산업 규모의 생산은, 비록 이들 화학물질 중 일부, 예를 들어, 리보플라빈, 비타민 B6, 판토테네이트 및 바이오틴이 미생물의 대규모 배양에 의해서도 생산된다 하더라도 세포와 무관한 화학 합성에 주로 의존한다. 비타민 B12만이 발효에 의해 전적으로 생성되는데, 이는 비타민 B12의 합성의 복잡성 때문이다. 시험관내 방법론은 재료 및 시간, 종종 많은 비용의 상당한 투입을 요구한다.
C. 퓨린, 피리미딘, 뉴클레오시드 및 뉴클레오티드의 대사 및 용도
퓨린 및 피리미딘 대사 유전자 및 이들의 대응하는 단백질은 종양 질환 및 바이러스 감염의 치료에 중요한 표적이다. 용어 "퓨린" 또는 "피리미딘"은 핵산, 조효소 및 뉴클레오티드의 일부를 형성하는 질소 함유 염기를 포함한다. 용어 "뉴클레오티드"는 질소 함유 염기, 오탄당 (RNA의 경우, 상기 당은 리보스이고; DNA의 경우, 상기 당은 D-데옥시리보스임) 및 인산으로 구성된, 핵산 분자의 기본 구조 단위를 포함한다. 용어 "뉴클레오시드"는 뉴클레오티드의 전구체로 작용하지만 뉴클레오티드가 갖는 인산 잔기가 없는 분자를 포함한다. 이들 분자의 생합성을 억제하거나 핵산 분자를 형성하기 위한 이들 분자의 동원을 억제함으로써, RNA 및 DNA 합성을 억제하는 것이 가능하고; 암세포에서 상기 활성을 표적화하여 억제함으로써 종양 세포의 분열 및 복제 능력을 억제할 수 있다. 또한, 핵산 분자를 형성하기 보다는 에너지 저장물 (즉, AMP) 또는 조효소 (즉, FAD 및 NAD)로 작용하는 뉴클레오티드도 있다.
이들 화학물질이 퓨린 및(또는) 피리미딘 대사에 영향을 줌으로써 상기 의학적 증상에 사용될 수 있다는 것은 여러 가지 문헌에 기재되어 있다 (예를 들어, Christopherson, R. I. and Lyons, S. D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents. "Med. Res. Reviews 10: 505-548). 퓨린 및 피리미딘 대사에 관여하는 효소의 연구는 예를 들어, 면역억제제 또는 항증식제로 사용할 수 있는 신약의 개발에 초점을 두고 있다 (Smith, J. L., (1995) "Enzymes in nucleotide synthesis." Curr. Opin. Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). 그러나, 퓨린 및 피리미딘 염기, 뉴클레오시드 및 뉴클레오티드는 여러 가지 정밀화학물질 (예를 들어, 티아민, S-아데노실메티오닌, 폴레이트 또는 리보플라빈)의 생합성에 있어서 중간 산물로서의 용도, 세포를 위한 에너지 캐리어 (예를 들어, ATP 또는 GTP)로서의 용도, 및 통상적으로 향 상승제 (예를 들어, IMP 또는 GMP)로 사용되는 화학물질 자체를 위한 용도 또는 여러 의학적 적용을 위한 용도를 갖는다 (예를 들어, 문헌 [Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561-612] 참조). 또한, 퓨린, 피리미딘, 뉴클레오시드 또는 뉴클레오티드 대사에 관여하는 효소를 표적으로 사용하여 살진균제, 제초제 및 살충제를 비롯한, 농작물 보호용 화학물질을 개발하는 연구가 증가하고 있다.
세균에서 이들 화합물 대사의 특징이 규명되어 있다 (전반적인 내용은 예를 들어, 문헌 [Zalkin, H. and Dixon, J. E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287]; 및 [Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York]을 참조함). 퓨린의 대사는 집중적인 연구의 대상이고, 세포의 정상적인 기능에 필수적이다. 고등 동물의 손상된 퓨린 대사는 심각한 질환 (예를 들어, 통풍)을 초래할 수 있다. 퓨린 뉴클레오티드는 구아노신-5'-모노포스페이트 (GMP) 또는 아데노신-5'-모노포스페이트 (AMP)를 생성시키는 중간체 화합물인 이노신-5'-포스페이트 (IMP)를 통해 일련의 단계에서 리보스-5-포스페이트로부터 합성되고, 이들 GMP 또는 AMP로부터 뉴클레오티드로 사용되는 트리포스페이트 형태가 용이하게 형성될 수 있다. 이들 화합물은 에너지 저장물로도 사용되므로, 이들의 분해는 세포에서 다수의 다양한 생화학적 과정을 위한 에너지를 공급한다. 피리미딘 생합성은 리보스-5-포스페이트로부터의 유리딘-5'-모노포스페이트 (UMP)의 형성에 의해 진행된다. 그 다음, UMP가 시티딘-5'-트리포스페이트 (CTP)로 전환된다. 이들 모든 뉴클레오티드의 데옥시 형태는 뉴클레오티드의 디포스페이트 리보스 형태가 뉴클레오티드의 디포스페이트 데옥시리보스 형태로 전환되는 1 단계 환원 반응에서 생성된다. 인산화 후에, 이들 분자는 DNA 합성에 참여할 수 있다.
D. 트레할로스의 대사 및 용도
트레할로스는 α,α-1,1 결합에 의해 결합된 두 가지 당 분자로 구성된다. 트레할로스는 감미료, 건조 또는 동결 식품용 첨가제로서 식품 산업, 및 음료 산업에 통상적으로 사용된다. 그러나, 제약 산업, 또는 화장품 산업 및 생물공학 산업에도 사용된다 (예를 들어, 문헌 [Nishimoto et al., (1998) 미국 특허 제5,759,610호; Singer, M. A. and Lindquist, S. (1998) Trends Biotech. 16: 460467; Paiva, C. L. A. and Panek, A. D. (1996) Biotech. Ann. Rev. 2: 293-314]; 및 [Shiosaka, M. (1997) J. Japan 172: 97-102] 참조). 트레할로스는 다수의 미생물 효소에 의해 생성되고 주위 배지내로 자연적으로 방출되는데, 트레할로스는 당업계에 공지된 방법에 의해 상기 배지로부터 단리될 수 있다.
II. 씨. 글루타미쿰에서의 유전자 안정성, 단백질 합성 및 단백질 분비
씨. 글루타미쿰과 같은 세포로부터 관심있는 화합물을 생산하는 과정은 다수의 개별 과정이 축적된 것이며, 이들 과정은 서로 관련되어 있고, 이들 각각은 세포로부터의 전체적인 상기 화합물 생산 방출에 중요한 과정이다. 세포를 1종 이상의 화학물질을 과다생성하도록 변형시키는 경우, 이들 각각의 과정은 세포의 생화학적 장치가 상기 유전자 조작과 양립할 수 있다는 것을 보장한다. 특히 중요한 세포 메카니즘은 세포로 도입된 유전자(들)의 안정성, 돌연변이 유전자의 정확하게 전사 및 번역되는 능력 (코돈 이용도 (codon usage) 포함) 및 돌연변이 단백질 생성물의 정확하게 폴딩 및(또는) 분비되는 능력을 포함한다.
A. 세균의 복구 및 재조합 시스템
세포는 UV 조사, 산소-유리 라디칼 및 알킬화와 같은 핵산 손상 제제에 자주 노출된다. 또한, DNA 중합효소의 작용에도 불구하고 에러가 발생하고 있다. 세포는 유전자 안정성 (정상적인 증식 및 대사 과정 중 세포 기능에 필요한 유전자가 손상되지 않도록 보장함)과 유전자 다양성 (변화하는 환경에 세포가 적응할 수 있도록 함) 사이에서 평형을 유지하여야 한다. 따라서, 대부분의 세포는 DNA 복구 및 DNA 재조합에 대해 별개의 경로를 함유하며, 단 이들은 서로 연결되어 있다. DNA 복구는 손상을 직접 복구하거나 손상된 부위를 잘라내어 정확한 서열로 대체함으로써 DNA 분자 내의 에러를 엄격하게 교정한다. DNA 재조합 시스템 또한 핵산 분자를 복구하는데, DNA 양쪽 가닥 모두가 손상되어 어느 한쪽 가닥을 다른쪽 가닥을 교정하기 위한 주형으로 사용할 수 없는 손상만을 복구한다. 재조합 복구 및 SOS 반응은 손상 부위 내 또는 주변에서의 전도, 결실 또는 다른 유전자 재배열을 용이하게 일으킬 수 있으며, 이는 이어서 환경 변화 또는 스트레스에 적응하는 세포 능력에 기여할 수 있는 게놈 불안정성을 어느 정도 촉진한다.
고-충실도 복구 메카니즘은 DNA 손상의 직접 전도 및 상기 손상의 절단 및 상보적인 가닥에 코딩된 정보를 이용한 재합성을 포함한다. 상기 손상의 직접 전도는 본래 손상된 DNA의 반대편에 활성인 효소를 필요로 한다. 예를 들어, DNA-복구 메틸트랜스퍼라제의 작용은 부정확한 DNA 메틸화를 교정하고, 데옥시리보디피리미딘 포토리아제의 활성은 UV 조사에 의해 생성된 뉴클레오티드 다이머를 빛의 존재하에 상응하는 뉴클레오티드로 다시 절단함으로써 상기 다이머를 복구할 수 있다 (문헌 [Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, wiley: New York] 및 이 문헌에 포함된 참고문헌 참조).
광역 손상을 정확하게 복구하는 것은 특수화된 복구 메카니즘을 필요로 한다. 이 메카니즘은 미스매치 (mismatch) 복구 및 엑시젼 (excision) 복구 시스템을 포함한다. 각 염기에서의 손상은 우선 당 결합을 절단한 후 손상된 부위의 DNA 주쇄를 절단하여 손상된 염기 자체를 제거하는 여러 절단 반응에 의해 복구될 수 있다. 마지막으로, DNA 중합효소 및 DNA 리가제가 두번째 DNA 가닥을 주형으로 사용하여 절단된 부위를 채우고 연결시킨다. 이중 나선의 형태를 변형시키는 보다 실질적인 DNA 손상은 헬리카제 II, DNA 중합효소 I, UvrA, UvrB 및 UvrC 단백질들이 함께 손상된 부위에서 이중 나선의 단일 가닥을 절단하고, ATP-의존성 방식으로 손상된 영역을 풀어주고, 손상된 영역을 잘라내고, 다른 가닥을 주형으로 사용하여 손실된 영역을 채우는 ABC 시스템에 의해 교정될 수 있다. 마지막으로, DNA 리가제가 단일-가닥 파손 부위를 연결한다. 또한, G-T 미스매치에 특이적인 시스템 (이 시스템에는 Vsr 단백질이 관련되어 있음) 및 두 가닥의 잘못된 복구에 의한 소수 결실/삽입 에러에 대해 특이적인 복구 시스템 (이 시스템에는 메틸화-조절 경로가 관련되어 있음)이 존재한다.
또한 세균에서 광범위한 DNA 손상을 복구하는데 통상적으로 사용되는 저-충실성 복구 시스템이 존재한다. 이중-가닥 복구 및 재조합은 DNA 양쪽 가닥에 영향을 미치는 손상의 경우에 수행된다. 이러한 상황에서는 다른 가닥을 주형으로 사용하여 손상을 복구하는 것이 가능하다. 따라서, 복구 시스템은 상동성 DNA 분자 상에서 손상된 영역과 다른 복제 영역 사이에 이중-교차결합이 형성되는 사건을 포함한다. 박테리아는 빠르게 분열하여 통상적으로 게놈 DNA의 2차 카피가 사용가능하기 때문에, 상기 사건은 실제로 세포 분열이 일어나기 전에 가능하다. 상기 교차 사건은 용이하게 전도, 복제, 결실, 삽입 및 다른 유전자 재배열을 유도할 수 있기 때문에 유기체의 전체적인 유전자 불안정성을 증가시킨다.
SOS 반응은 DNA 손상이 DNA 중합효소의 진행을 중단시키기 충분하여 복제를 계속할 수 없는 경우에 활성화된다. 이러한 상황 하에서는 단일-가닥 DNA가 존재한다. RecA 단백질은 단일-가닥 DNA에 결합하여 활성화되며, 이 활성화된 형태는 LexA 리프레서 (UvrA, UvrB, UvrC, 헬리카제 II, DNA pol III, UmuC 및 UmuD 포함)를 활성화시켜 20개를 초과하는 유전자의 전사 장벽을 제거한다. 이 효소들의 결합된 활성이 DNA pol III가 복제를 계속하기에 충분하도록 단절 영역을 채운다. 그러나, 이 단절은 존재해서는 안되는 염기로 채워지기 때문에 이런 유형의 복구는 에러-프론 (error-prone) 복구를 유도하여 전반적으로 세포의 유전자 불안정성에 기여한다.
B. 트랜스포손
상기 언급한 고-충실성 또는 저-충실성 시스템은 DNA 손상을 복구해야만 한다. 특정 상황 하에서, 이러한 복구은 부가적인 유전자 재배열을 포함할 수 있다. 또한, 다수의 세균 세포는 특이적으로 상기 유전자 재배열을 유발하는 메카니즘을 갖는다. 상기 메카니즘 중 특히 잘 알려진 예는 트랜스포손이다.
트랜스포손은 염색체 내에서 또는 염색체외 DNA 조각 (예를 들어, 플라스미드)과 염색체 사이에서 한 부위에서 다른 부위로 이동할 수 있는 유전자 요소이다. 전위는 여러 방식으로 수행될 수 있다. 예를 들어, 전위가능한 요소를 도너 부위로부터 잘라내어 표적 부위로 삽입될 수 있거나 (비복제성 전위), 별법으로, 전위가능한 요소가 도너 부위로부터 표적 부위로 복제되어 결과적으로 상기 요소의 두 개 카피가 생성될 수 있다 (복제성 전위). 도너 부위의 서열과 표적 부위의 서열은 통상적으로 관련되어 있지 않다.
상기 전위 사건은 다양한 가능한 결과를 갖는다. 전위가능한 요소가 유전자 내에 삽입되면 상기 유전자가 파괴되고, 이것은 통상적으로 상기 유전자의 기능을 완전히 제거한다. 상기 유전자를 감싸는 DNA에서 일어나는 삽입은 코딩 서열 자체를 손상시킬 수는 없지만 상기 유전자의 조절에 근본적인 영향을 미칠 수 있기 때문에 유전자의 발현에도 영향을 미친다. 게놈의 다른 부분에 위치하는 전위가능한 요소의 두 카피 사이에서 발생하는 재조합 사건은 게놈 단편의 결실, 복제, 전도, 전위 또는 증폭을 유도할 수 있다. 다양한 리플리콘 (replicon)은 융합될 수도 있다.
가장 단순한 트랜스포손-유사 유전자 요소는 삽입 (IS) 요소로 언급된다. IS 요소는 코딩 영역을 함유하지 않으며 역전된 반복 서열로 둘러싸인 각 말단 상에 위치하는 다양한 길이 (단, 통상적으로 1500개 염기 미만)의 뉴클레오티드 영역을 함유한다. IS 요소는 활성이 탐지될 수 있는 어떠한 단백질도 코딩하기 않기 때문에, IS 요소의 존재는 통상적으로 IS 요소가 삽입된 하나 이상의 유전자 기능 손실에 의해서만 관찰된다.
트랜스포손은 IS 요소와는 달리 반복 서열에 의해 분단되며 1종 이상의 단백질을 코딩할 수 있는 이동성 유전자 요소이다. 이 반복 영역이 IS 성분을 포함하는 것이 드문 일은 아니다. 트랜스포손-코딩 단백질은 통상적으로 트랜스포자제 (한 부위로부터 다른 부위로의 트랜스포손 이동을 촉매하는 단백질) 및 항생제 내성 유전자이다. 전위가능한 요소의 메카니즘 및 조절은 당업계에 공지되어 있으며, 예를 들어, 문헌 [Lengeler et al. (1999) Biology of Prokaryotes, Thieme verlag: Stuttgart, pp. 375-361]; [Neidhardt et al. (1996) Escherichia coli and Salmonella, ASM Press: washington, D.C.]; [Sonenshein, Al.L., et al., Editors, (1993) Bacillus subtilis, ASM Press, washington, D.C.]; [Voet, D., and voet, J.G. (1992) Biochemie, VCH: weinheim, pp. 985-990]; [Brock, T.D., and Madigan, M.T. (1991) Biology of Mocroorganisms, 6th edition, Prentice hall: New York, pp. 267-269]; 및 [Kleckner, N. (1990) "Regulation of transposition in bacteria", Annu. Rev. Biochem. 61:297-327]에 기재되어 있다.
C. 전사
세균의 유전자 발현은 주로 전사 수준에서 조절된다. 전사 장치는 두 개의 군, 즉 RNA 중합효소 (DNA-전사 효소를 작동시킴) 및 시그마 인자 (RNA 중합효소를 상기 인자를 인식하는 특이적인 프로모터 DNA 서열에 직접 연결하여 유전자 전사를 조절함)로 분류할 수 있는 다수의 단백질을 포함한다. RNA 중합효소와 시그마 인자의 조합은 활성화된 복합체인 RNA-중합효소 완전효소 (holoenzyme)를 형성한다. 코리네박테리아와 같은 그람-양성 세균은 한가지 유형의 RNA 중합효소만을 함유하지만 여러 프로모터, 증식 단계, 환경 조건, 기질, 산소 수준, 수송 과정 등에 특이적인 다수의 상이한 시그마 인자를 함유하며 그 결과 미생물은 여러 환경 및 대사 조건에 적응할 수 있다.
프로모터는 RNA-중합효소 완전효소에 대해 도킹 부위를 제공하는 특이적인 DNA 서열이다. 다수의 프로모터 성분이 상동성 검색에 의해 검출될 수 있는 서열 성분을 보존하고 있으며, 다르게는, 특정 유전자에 대한 프로모터 영역은 프라이머 신장과 같은 표준 기술을 이용하여 확인할 수 있다. 다수의 그람-양성 박테리아 프로모터 영역이 공지되어 있다 (예를 들어, 문헌 [Sonenshein, A.L., Hoch, J.A., and Losick, R., Editors, (1993) Bacillus subtilis, ASM Press: washington, D.C.] 참조).
대다수의 억제 또는 활성 메카니즘은 프로모터 전사 조절에 영향을 미친다. 프로모터에 결합하는 특이적인 조절 단백질은 RNA 완전효소의 결합을 차단 (리프레서) 또는 지지 (활성인자)하여 전사를 조절할 수 있다. 이 리프레서 및 활성인자 분자의 결합은 이어서 이들의 단백질 또는 다른 대사 화합물과 같은 다른 분자와의 상호작용에 의해 조절된다. 다르게는, 전사는 연장 또는 종결 과정과 같은 과정에 영향을 미치는 인자들의 의해 조절될 수 있다 (예를 들어, 문헌 [Sonenshein, A.L., Hoch, J.A., and Losick, R., Editors, (1993) Bacillus subtilis, ASM Press: Washington, D.C.] 참조). 다수의 환경 또는 대사 신호에 대해 반응하여 유전자 전사를 조절하는 능력은 세포가 유전자가 발현될 수 있는 시기와 특정 시점에 세포에 존재할 수 있는 유전자 생성물의 양을 조절할 수 있도록 한다. 이것은 이어서 불필요한 에너지 낭비 또는 드물게 나타날 수 있는 중간체 또는 보조인자의 불필요한 사용을 방지한다.
D. 번역 및 아미노아실-tRNA 합성효소
번역은 RNA 분자에 함유된 정보에 따라 아미노산으로부터 폴리펩티드를 합성하는 과정이다. 이 과정의 주요 성분은 리보솜 및 특이적인 개시 또는 연장 인자 (예를 들어, IF1-3, INVENTIVE-G 및 EFTu)들이다 (예를 들어, 문헌 [Sonenshein, A.L., Hoch, J.A., and Losick, R., Editors, (1993) Bacillus subtilis, ASM Press: washington, D.C.] 참조).
mRNA 분자의 각각의 코돈은 특정 아미노산을 코딩한다. mRNA는 전이-RNA (tRNA) 분자를 통해 아미노산으로 전환된다. 이 분자들은 L-형 삼차원 구조에서 신장 영역으로 존재하는 RNA 단일 가닥 (60 내지 100 염기) 또는 "암 (arm)"으로 구성되어 있다. 이 암 중 하나는 mRNA 분자 상의 특정 코돈 서열과 염기쌍을 형성한다. 두번째 암은 특정 아미노산 (코돈에 의해 코딩됨)과 특이적으로 상호작용한다. 다른 tRNA 암은 가변성 암 (TψC 암 (티미딜레이트 및 슈도우리딜레이트 변형을 운반함) 및 D 암 (디히드로우리딘 변형을 운반함)을 포함한다. D 암 구조의 기능은 아직까지 알려져 있지 않지만 tRNA 분자들 사이에 이들이 보존되어 있다는 것은 상기 구조가 단백질 합성에서 기능을 수행한다는 것을 제안하다.
아미노아실-tRNA 합성효소로 언급되는 효소 족은 핵산-기재 tRNA 분자에 대해 정확한 아미노산과 쌍을 이루도록 작용해야 한다. 매우 다양한 효소들이 존재하며, 이들 각각은 특정 tRNA 및 특정 아미노산에 대해 특이적이다. 상기 효소는 tRNA-아데노신-리보스 말단의 3'-히드록실 잔기를 아미노산에 2-단계 반응으로 결합시킨다. 첫번째 단계에서, 효소는 ATP 및 아미노산과의 반응을 통해 활성화되어아미노아실-tRNA-합성효소-아미노아실-아데닐레이트 복합체를 형성한다. 두번째 단계에서는, 아미노아실기가 효소로부터 고-에너지 상태를 유지하고 있는 표적 tRNA로 전달된다. tRNA 분자는 mRNA 분자 상에 있는 자신의 인식 코돈에 결합된 후에 tRNA-결합 고 에너지 아미노산을 리보솜과 접촉시킨다. 리보솜 내에서, 아미노산-로딩 tRNA (아미노아실-tRNA)는 자신의 아미노산을 발생기 폴리펩티드 쇄에 결합시키는 tRNA 분자 (펩티딜 tNRA)를 운반하는 2차 부위 (P 부위) 뿐만 아니라 결합 부위 (A 부위)를 차지한다. 아미노아실 tRNA 상의 활성화된 아미노산은 발생기 폴리펩티드 쇄 상에서 이 아미노산과 다음 아미노산 사이에 펩티드 결합을 동시에 형성하기에 충분한 반응성을 갖는다. GTP 가수분해는 리보솜의 A 부위로부터 P 부위로 폴리펩티드 쇄와 함께 로딩된 tRNA를 전달하는 에너지를 제공하며, 이 과정은 중단 코돈에 다다를 때까지 반복된다.
변역을 조절할 수 있는 다른 단계들이 다수 존재한다. 이 단계들은 리보솜의 mRNA로의 결합, mRNA 2차 구조의 존재, 코돈 이용도 또는 특정 tRNA의 빈도를 포함한다. 감쇠 (attenuation)와 같은 특이적인 조절 메카니즘 또한 번역 수준에서 작용할 수 있다. 이 메카니즘 중 다수에 대한 심도 싶은 개요가 예를 들어 문헌 [vellanoweth, R.L. (1993) "Translation and its Regulation", in: Bacillus subtilis and other Gram positive bacteria, Sonenshein, A.L., et al., Editors, ASM Press: washington, D.C., pp. 699-711] (및 이 문헌에 포함된 참고문헌 참조)에서 찾을 수 있다.
E. 단백질 폴딩 및 단백질 분비
단백질의 리보솜 합성은 단백질이 정상적으로 기능할 수 있기 전에 삼차원 형태를 채택해야 하는 폴리펩티드 쇄를 유도한다. 삼차원 구조는 폴딩 과정에 의해 달성된다. 폴리펩티드 쇄는 가요성이고, (이론상) 보다 안정한 삼차원 구조를 유도하는 구성을 채택할 때까지 용액 중에서 용이하고 자유롭게 움직인다. 그러나, 때로는 환경 조건 (예를 들어, 시스템 내에 존재하는 역학 에너지가 고온일 때 단백질이 최소의 에너지를 갖는 안정한 구조를 형성하는 것을 보다 어렵게 함) 또는 단백질 자체의 유형 (예를 들어, 서로 근접하게 위치하는 단백질 내의 소수성 영역이 수용액으로부터 자신들을 침전시켜 참착된 유형) 때문에 단백질을 정확하게 폴딩시키기 어렵게 된다.
단백질 폴딩을 촉매하거나, 동반하거나, 다르게 지지할 수 있으며, 번역과 동시에 또는 번역 후에 합성되는 단백질-유사 인자들이 확인되어 있다. 이러한 단백질 폴딩 분자들로는 프롤릴-펩티딜 이소머라제 (예를 들어, 촉진 인자, 시클로필린 및 FKBP 상동체) 및 또한 열충격 단백질 군의 단백질 (예를 들어, DnaK, DnaJ, GroEL, 열충격 소단백질, HtpG) 및 Clp 족 구성원 (예를 들어, ClpA, ClpB, ClpW, ClpP 및 ClpX)이 있다. 이 단백질 중 다수는 단백질 폴딩, 단백질 전위 및 단백질 과정에서의 기능 할 뿐만 아니라 세포 생존에도 중요하며, 이들은 흔히 전체적인 단백질 합성 조절을 위한 표적으로 제공된다 (예를 들어, 문헌 [Bukau, B. (1993) Molecular Microbiology 9(4):671-680; Bukau, B., and Horwich, A.L. (1998) Cell 92(3):351-366]; [Hesterkamp, T., Bukau, C. (1996) FEBS Lett. 389(1):32-34; Yaron, A., Naider, F. (1993) Critical Reviews in Biochemistry and Molecular Biology 28(1):31-81]; [Scheibel, R., Buchner, J. (1998) Biochemical Pharmacology 56(6):675-682]; [Ellis, R.J., hartl, F.U. (1996) FASEB Journal 10(1):20-26; Wawrzynow, A., et ale (1996) Molecular Microbiology 21(5):895-899]; [Ewalt, K.L., et ale (1997) Cell 90(3):491-500] 참조).
앞서 확인된 샤페론 (chaperone)은 두 가지 방식으로 작용한다. 이들은 폴리펩티드에 결합하여 폴리펩티드를 안정화시키거나, 붕괴되지 않고 폴딩이 일어날 수 있는 환경을 제공한다. 전자의 군 (예를 들어, DnaK, DnaJ 및 열충격 단백질 포함)은 발생기 또는 잘못 폴딩된 폴리펩티드 (ATP 가수분해에 의해 빈번하게 나타남)에 직접 결합한다. 샤페론 결합은 폴리펩티드가 다른 폴리펩티드와 침착되는것을 방지하며, 이미 형성된 경우에 이 침착물의 분리를 추진할 수 있다. 2차 샤페론 GrpE (ADP-ATP 교환을 가능하게 함)과의 상호작용 후에, 폴리펩티드는 용융-구체 (molten-globule) 상태로 방출되어 폴딩될 수 있다. 폴딩이 잘못되는 경우, 샤페론은 잘못 폴딩된 단백질에 재결합하여 폴딩되지 않은 상태로 돌려놓는다. 이러한 주기는 단백질이 정확하게 폴딩될 때까지 반복된다. 폴리펩티드에 단순하게 결합하는 샤페론의 1차 군과는 달리, 2차 군 (예를 들어, GroEL/ES)은 폴리펩티드에 결합할 뿐만 아니라 폴리펩티드를 완전히 감싸서 주변 환경으로부터 보호한다. GroEL/ES 복합체는 내부 표면이 소수성인 이중으로 적층된 14-원 고리와 7-원 고리로 만들어진 "리드 (lid)"로 구성된다. ATP-의존성 반응에서, 폴리펩티드는 다른 폴리펩티드의 의해 붕괴되지 않고 폴딩될 수 있는 상기 복합체 중앙에 있는 채널로 이동시킨다. 잘못 폴딩된 단백질은 복합체로부터 방출되지 않는다.
단백질 폴딩에서 중요한 단계는 이황 결합의 형성이다. 단백질의 서브유니트 내 또는 서브유니트 사이이 이황 결합은 단백질 안정성에 중요하다. 이황 결합은 수용액에서 용이하게 형성되며 환원 환경의 도움 없이 잘못된 이황 브릿지 (bridge) 형성을 되돌리기 어렵다. 정확한 이황 브릿지를 형성하는 상기 과정을 지지하기 위해 대다수 세포의 시토졸은 글루타티온 또는 티오레독신과 같은 티올 함유 분자 및 이들의 상응하는 산화/환원 시스템을 함유한다 (Loferer, H., Hennecke, H. (1994) Trends in Biochemical Sciences 19(4):169-171).
그러나, 특정 시점에서는 발생기 폴리펩티드 쇄의 폴딩이 바람직하지 않다 (예를 들어, 이 단백질이 분비되어야 하는 경우). 폴딩 과정은 통상적으로 상기 단백질의 중앙에 위치하는 단백질의 소수성 영역 (수용액으로부터 제거됨) 및 상기 단백질의 외피에 존재하는 친수성 영역에서 발생한다. 비록 이러한 구성 배열이 단백질의 높은 안정성을 초래한다고 하더라도, 이것은 막의 소수성 코어가 단백질의 소수성 외면으로 적합하지 않기 때문에 막을 통한 단백질의 전위를 보다 어렵게 한다. 따라서, 세포에 의해 합성되어 세포의 외면으로 분비되는 단백질 (예를 들어, 세포 표면 효소 및 막 수용체) 또는 세포에 의해 합성되어 단백질 자체가 막으로 삽입되는 단백질 (예를 들어, 수송 단백질 및 채널 단백질)들은 통상적으로 폴딩되기 전에 분비되거나 삽입된다. 발생기 폴리펩티드 쇄의 침착을 방지하는 동일한 샤페론은 또한 이들을 더 이상 필요로 하지 않을 때까지 폴리펩티드의 폴딩을 방지한다. 따라서, 이러한 단백질들은 발생기 폴리펩티드를 세포 내의 적합한 위치 (여기서, 발생기 폴리펩티드가 제거되어 폴딩 또는 폴리펩티드를 분비하거나 막으로의 삽입을 지지하는 수송 시스템으로의 단백질 이동을 가능하게 함)로 "에스코트"할 수 있다.
진화 과정 동안, 특이적인 전구서열 (나중에 절단되어 단백질로부터 제거됨)로 단백질을 인식하고, 결합하고, 수송하며 진행시키는 특성화된 단백질 기구가 형성되었다. 상기 장치는 sec (유형 II 분비) 시스템으로 총칭되는 수많은 단백질을 포함한다 (전반적인 내용은, 문헌 [Gilbert, M., et al.(1995) Critical Reviews in Biotechnology 15(1):13-39 및 이 문헌에 포함된 참고문헌]; [Freud1, R. (1992) Journal of Biotechnology 23(3):231-240 및 이 문헌에 포함된 참고문헌]; [Neidhardt, F.C., et al.(1996) E. coli and Salmonella, ASM Press: washington, D.C., pp. 967-978]; [Binet, R., et al. (1997) gene 192(1):7-11 und Rapoport, T.A. (1986) Critical Reviews in Biochemistry 20(1):73-137 및 이 문헌에 포함된 참고문헌] 참조). sec 시스템은 샤페론 (예를 들어, SecA 및 SecB), 내재성 막 단백질 (전위효소 (예를 들어, SecY, SecE 및 SecG)라고도 언급됨) 및 신호 펩티다제 (예를 들어, LepB)를 포함한다. 분비를 유도하는 프로서열의 발생기 폴리펩티드는 이를 세포 막 내피 상의 SecA로 전달하는 SecB에 의해 결합된다. SecA는 ATP 가수분해 후에 프로서열에 결합하고, 막으로 삽입되며, 또한 막을 통해 폴리펩티드 단편으로 분리된다. 나머지 폴리펩티드는 SecY, SecE 및 SecG와 같은 전위효소 복합체를 통해 막으로 유도된다. 마지막으로, 신호 펩티다제는 절단에 의해 프로서열을 제거하고, 폴리펩티드는 막의 세포외 측면에서 유리되어 존재하며, 여기에서 자발적으로 폴딩된다.
sec-독립성 분비 메카니즘 또한 공지되어 있다. 예를 들어, 신호 인식 입자-의존성 경로는 리보솜 진행을 중단시켜 합성 과정 동안 발생기 폴리펩티드에 대한 신호를 인식하는 입자 (SRP) 단백질을 함유한다. 이어서, 막의 내피 상의 SRP에 대한 수용체는 리보솜-폴리펩티드-SRP 복합체와 결합한다. GTP 가수분해는 복합체를 합성 과정 동안 리보솜에 의해 막을 따라 유도되는 폴리펩티드 상의 sec-전위효소 복합체로 전달하는데 필요한 에너지를 제공한다. 몇몇 단백질에만 특이적인 분비 메카니즘이 존재한다는 것이 공지되어 있다.
III. 본 발명의 요소 및 방법.
본 발명은 적어도 부분적으로 본원에서 SES 핵산 및 SES-단백질 분자로 언급되며, 씨. 글루타미쿰에서의 DNA 복구 또는 재조합, 씨. 글루타미쿰 DNA의 전위 또는 기타 재배열, 씨. 글루타미쿰에서의 유전자 발현 (즉, 전사 또는 번역 과정), 이 미생물의 단백질 폴딩 또는 단백질 분비에 참여하는 새로운 분자를 검출하는 것을 기초로 하고 있다. 한 실시양태에서, SES 분자는 코리네박테리움 글루타미쿰에서의 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비에 참여한다. 바람직한 실시양태에서, 본 발명 SES 분자의 활성은 상기 미생물에 의한 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현, 단백질 폴딩 또는 단백질 분비와 관련된 관심있는 정밀화학물질의 생산에 영향을 미친다. 특히 바람직한 실시양태에서, 본 발명 SES 분자의 활성은 조절되어 본 발명의 SES 단백질이 관련되어 있는 씨. 글루타미쿰의 세포 과정 (예를 들어, DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현, 단백질 폴딩 또는 단백질 분비) 활성을 또한 변형시켜 씨. 글루타미쿰에 의한 관심있는 정밀화학물질의 생산 수율, 생산성 및(또는) 생산 효율을 직접 또는 간접적으로 조절하게 된다.
용어 "ha 단백질" 또는 "ha 폴리펩티드"는 씨. 글루타미쿰의 유전자 안정성, 유전자 발현, 단백질 폴딩 또는 단백질 분비와 연관된 수많은 세포 과정에 관련된 단백질을 포함한다. 예를 들어, SES 단백질은 씨. 글루타미쿰에서의 DNA 복구 또는 재조합 메카니즘, 씨. 글루타미쿰 유전자 물질의 재배열 (예를 들어, 트랜스포존에 의해 매개되는 것), 상기 미생물에서의 전사 또는 번역, 씨. 글루타미쿰에서의 단백질 폴딩 조절 (예를 들어, 샤페론의 활성) 또는 씨. 글루타미쿰으로부터의 단백질 분비에 관련되어 있다 (예를 들어, sec 시스템). SES 단백질의 예에는 표 1 및 부록 A에 나열된 SES 유전자에 의해 코딩되는 단백질들이 포함된다. 용어 "ha 유전자" 또는"ha 핵산 서열"은 코딩 영역 및 상응하는 비번역된 5' 및 3' 서열을 포함하는 SES 단백질을 코딩하는 핵산 서열을 포함하는 영역을 포함한다. SES 유전자의 예는 표 1에 나열된 유전자들이다. 용어 "생산" 또는 "생산성"은 당업계에 공지되어 있으며, 소정의 시간 및 소정의 발효 용적내에서 생성되는 발효 산물의 농도 (예를 들어, 1시간에 1리터 당 kg 산물)가 포함된다. 용어 "생산 효율"에는 특정 생성물 농도를 달성하는데 요구되는 시간(예를 들어, 세포가 특정 비율의 정밀화학물질 생성물을 얻는데 걸리는 시간)이 포함된다. 용어 "수율" 또는 "산물/탄소 수율"은 당업계에 공지되어 있으며, 탄소 공급원의 생성물 (즉, 정밀화학물질)로의 전환 효율이 포함된다. 이 용어는 일반적으로는, 예를 들어, 탄소 공급원 kg 당 생성물 kg으로 표기된다. 화합물의 수율 또는 생산율을 증대시킴으로써, 소정의 시간 동안 얻어지는 수득량의 배양물 중 화합물의 회수된 분자의 양 또는 적합한 분자의 양은 증가하게 된다. 용어 "생합성" 또는 "생합성 경로"는 당업계에서 공지되어 있고, 세포에 의한 중간체 화합물로부터의 화합물, 바람직하게는 유기 화합물의 합성을 포함하며, 다단계의 고도로 조절되는 과정일 수 있다. 용어 "분해" 또는 "분해 경로"는 당업계에 공지되어 있으며, 세포에 의한 화합물, 바람직하게는 유기 화합물의 분해 생성물 (일반적으로 말해서, 더 작거나 덜 복잡한 구조의 분자)로의 분해를 포함하며, 다단계의 고도로 조절되는 과정일 수 있다. 용어 "대사"는 당업계에 공지되어 있으며, 유기체에서 일어나는 전체적인 생화학 반응을 포함한다. 이어서, 특정 화합물의 대사 (예를 들어, 글리신과 같은 아미노산의 대사)는 상기 화합물과 관련된 세포내의 모든 생합성, 변형 및 분해 경로를 포함한다. 용어 "DNA 복구"는 당업계에 공지되어 있으며, DNA 내의 에러 (자외선 조사, 메틸라제, 저-충실성 복제 또는 뮤타젠 (mutagen) (이에 제한되지는 않음)과 같은 손상에 의한 에러)를 잘라내어 복구하는 세포 메카니즘을 포함한다. 용어 "재조합" 또는 "DNA 재조합"은 당업계에 공지되어 있으며, DNA 분자 양쪽 가닥에 영향을 미치는 확장된 DNA 손상을 동일 세포 내에서 DNA 분자의 다른 손상되지 않은 카피와의 상동성 재조합을 통해 복구하는 세포 메카니즘을 포함한다. 이러한 복구는 통상적으로 저 충실성을 갖고, 유전자 재배열을 유발할 수 있다. 용어 "트랜스포손"은 당업계에 공지되어 있으며, 미생물의 게놈에 무작위적으로 삽입될 수 있으며 유전자 또는 이들의 조절 영역의 붕괴, 또는 복제, 전도, 결실 및 기타 유전자 배열을 유발할 수 있는 DNA 성분을 포함한다. 용어 "단백질 폴딩"은 당업계에 공지되어 있으며, 안정한 활성 삼차원 배열에 도달할 때까지 여러 삼차원 배열을 통한 폴리펩티드 쇄의 이동을 포함한다. 이황 결합 형성 및 주변 수용액으로부터의 소수성 영역의 분리는 이 단백질 폴딩 과정에 대한 추진력 중 일부를 제공하며, 정확한 폴딩은 샤페론의 활성에 의해 향상될 수 있다. 용어 "분비" 또는 "단백질 분비"는 당업계에 공지되어 있으며, 분비 단백질을 세포 막을 통해 세포 외부로 통과할 수 있도록 하는 시스템에서의 세포 내부로부터 세포 외부로의 단백질 이동을 포함한다.
다른 실시양태에서, 본 발명의 SES 분자는 씨. 글루타미쿰과 같은 미생물에서 정밀화학물질과 같은 관심있는 분자의 생산을 조절할 수 있다. 본 발명의 SES 단백질을 변형시켜 이 변형된 단백질을 함유하는 씨. 글루타미쿰 균주로부터 생산되는 정밀화학물질의 수율, 생산성 및(또는) 생산 효율에 직접 영향을 미칠 수 있는 다수의 메카니즘이 존재한다. 예를 들어, 자신의 수 또는 활성을 증가시키기 위한 전사 또는 번역에 직접 관련된 단백질 (예를 들어, 중합효소 또는 리보솜)의 조절은 전체 세포 전사 또는 번역 (또는 이들 과정의 속도)을 증가시킬 것이다. 이 증가된 세포 유전자 발현은 정밀화학물질의 생합성에 관련되어 1종 이상의 관심있는 화합물의 수율, 생산 또는 생산 효율을 증가시킬 수 있는 단백질을 포함해야 한다. 이러한 단백질의 조절을 변형시키기 위한 씨. 글루타미쿰의 전사/번역 단백질 기구의 변형은 또한 정밀화학물질 생산과 관련된 유전자의 발현을 증가시킬 수 있다. 펩티드 폴딩과 관련된 다수의 단백질의 활성 조절은 정확하게 기능하는 관심있는 단백질 (예를 들어, 정밀화학물질 생합성 단백질)의 가능성을 증가시켜 세포에서 정확하게 폴딩된 분자의 전체 생산량을 증가시킨다. 또한, 수 및 활성을 증가시키기 위한 씨. 글루타미쿰으로부터의 분비와 관련된 단백질을 돌연변이화하여 정밀화학물질을 용이하게 수득할 수 있는 발효 배양물로부터의 정밀화학물질 (예를 들어, 효소)의 분비를 증가시키는 것이 가능하다.
본 발명 SES 분자의 유전자 변형은 1종 이상의 정밀화학물질 생산을 간접적으로 조절할 수 있다. 예를 들어, 본 발명의 NA-복구 또는 DNA-재조합 단백질의 수 또는 활성을 증가시켜 DNA 손상을 탐지하여 복구하는 세포의 능력을 증가시킬 수 있다. 이것은 세포가 자신의 게놈 내의 돌연변이 유전자를 유지하는 능력을 증가시켜 미생물 배양 과정 동안 손실되지 않는, 유전적으로 씨. 글루타미쿰으로 도입된 트랜스유전자의 존재 가능성을 증가시킨다 (예를 들어, 정밀화학물질의 생합성을 증가시키는 단백질을 코딩함). 반면, 1종 이상의 DNA-복구 또는 DNA-재조합 단백질의 수 또는 활성을 감소시켜, 유기체의 유전자 불안정성을 증가시킬 수 있다. 이러한 조작은 상기 미생물이 도입된 돌연변이를 교정하지 않고 돌연변이유발에 의해 변형되는 능력을 향상시킬 것이다. 씨. 글루타미쿰에서 유전자 요소의 전위 또는 재배열에 관여하는 단백질 (예를 들어, 트랜스포손)의 경우에도 동일한 결과가 성립된다. 이들 단백질의 돌연변이화는 그들의 수 또는 활성을 증가시키거나 감소시켜 동시에 미생물의 유전자 안정성을 증가시키거나 감소시킬 수 있다. 이것은 씨. 글루타미쿰으로의 다른 돌연변이의 도입 가능성 및 도입된 돌연변이를 유지할 가능성에 중대한 영향을 미친다. 트랜스포손은 또한 씨. 글루타미쿰의 돌연변이유발; 트랜스포손 돌연변이유발에 의해 용이하게 수행될 수 있는 원치않는 유전자 (예를 들어, 관심있는 정밀화학물질의 분해에 관여하는 유전자)의 붕괴 뿐만 아니라 관심있는 유전자 (예를 들어, 정밀화학물질 생합성 유전자)의 복제를 가능하게 하는 적합한 메카니즘을 제공한다.
특정 환경 조건에 대응하여 전사 또는 번역은 조절하는 과정에 관여하는 1종 이상의 단백질 (예를 들어, 시그마 인자)을 조작하여 대규모 발효 배양시 나타나는 바람직하지 못한 환경 조건에서 단백질 합성이 지연되거나 중단되는 것을 방지한다. 이것은 유전자 발현을 증가시켜 상기 조건 하에서 정밀화학물질의 생합성을 증가시킬 수 있다. 이 분비 단백질 중 다수가 세포 생존에 중요한 기능을 갖는다 (예를 들어, 세포 표면 프로테아제 또는 세포 표면 수용체). 분비 경로를 변화시켜 상기 단백질을 세포 외 영역으로 보다 용이하게 수송함으로써 전체적인 세포 생존율을 증가시켜 다수의 씨. 글루타미쿰 세포가 대규모 배양시 정밀화학물질을 생산할 수 있게 된다. 또한 특정 세균 단백질의 분비 경로 (예를 들어, sec 시스템)이 막으로의 내재성 막 단백질 (예를 들어, 수용체, 채널, 포어, 또는 수송 단백질) 삽입 과정에 관여한다고 알려져 있기 때문에, 씨. 글루타미쿰으로부터의 단백질 분비에 관여하는 단백질의 활성을 조절하여 세포의 폐기물 제거 또는 필수적인 대사산물의 도입 능력에 영향을 미칠 수 있다. 이들 분비 단백질의 활성이 증가하면, 세포의 정밀화학물질 생산 능력 또한 증가한다 (영양소를 도입하거나 폐기물을 제거할 수 있는 수송 단백질/채널이 증가하기 때문). 상기 분비 단백질의 활성이 감소되면, 영양소가 관심있는 화합물이 과다생성하기에 충분하지 않게 되거나 폐기물이 상기 생합성을 방해할 수 있다.
본 발명의 핵산 서열을 제조하기에 적합한 시작점은 아메리칸 타입 컬쳐 컬렉션 (American Type Culture Collection)으로부터 얻을 수 있는 ATCC 13032라고 명명되는 코리네박테리움 글루타미쿰 균주의 게놈이다.
본 발명의 핵산 서열은 상기 핵산 서열로부터 통상적인 방법을 이용하여 표 1에서 언급한 변형을 통해 제조될 수 있다.
본 발명의 SES 단백질 또는 그의 생물학적으로 활성인 부분 또는 단편이 코리네박테리움 글루타미쿰에서의 DNA 복구 또는 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비에 참여할 수 있거나, 표 1에 기재된 활성을 하나 이상 가질 수 있다.
본 발명의 다양한 측면은 하기 소단락에 보다 상세히 기재되어 있다.
A. 단리된 핵산 분자
본 발명의 한 측면은 SES 폴리펩티드 또는 그의 생물학적으로 활성인 일부를 코딩하는 단리된 핵산 분자와 SES-코딩 핵산 (예를 들어, SES DNA)을 확인하거나 증폭시키기 위한 혼성화 프로브 또는 프라이머로 사용하기에 충분한 핵산 단편에 관한 것이다. 본원에 사용된 바와 같이, 용어 "핵산 분자"는 DNA 분자 (예를 들어, cDNA 또는 게놈 DNA), RNA 분자 (예를 들어, mRNA), 및 뉴클레오티드 유사체를 사용하여 생성되는 DNA 또는 RNA 유사체를 포함하는 의미이다. 이 용어에는 또한 유전자 코딩 영역의 3' 및 5' 말단에 위치하는 비번역 서열, 코딩 영역의 5' 말단 상류의 약 100개 이상의 뉴클레오티드 서열, 및 이 유전자의 코딩 영역의 3' 말단 하류의 약 20개 이상의 뉴클레오티드 서열이 포함된다. 핵산 분자는 단일 가닥 또는 이중 가닥일 수 있으며, 바람직하게는 이중 가닥 DNA이다. "단리된" 핵산 분자는 핵산의 천연 공급원에 존재하는 다른 핵산 분자로부터 분리되는 것이다. 바람직하게는, "단리된" 핵산은 이 핵산이 유도되는 유기체의 게놈 DNA에서 본래 핵산의 양측면에 있는 임의의 서열 (예를 들어, 핵산의 5' 및 3' 말단에 위치하는 서열)을 가지고 있지 않다. 예를 들어, 다양한 실시양태에서, 단리된 SES 핵산 분자는 이 핵산이 유도되는 세포 (예를 들어, 씨. 글루타미쿰 세포)의 게놈 DNA에서 본래 핵산 분자의 양측면에 있는 약 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb 또는 0.1 kb 보다 작은 크기의 뉴클레오티드 서열을 포함할 수 있다. 게다가, "단리된" 핵산 분자, 예를 들어, cDNA 분자는, 재조합 기술에 의해 생산되는 경우 다른 세포 물질 또는 배양 배지, 또는 화학적으로 합성되는 경우 화학물질 전구체 또는 다른 화학물질을 실질적으로 함유하지 않는다.
표준 분자 생물학 기술 및 본원에 제공되는 서열 정보를 이용하여, 본 발명의 핵산 분자, 예를 들어, 부록 A의 뉴클레오티드 서열을 갖는 핵산 분자 또는 그의 일부를 단리할 수 있다. 예를 들어, 부록 A의 전체 서열 또는 그의 일부를 혼성화 프로브로 사용하고 표준 혼성화 기술 (예를 들어, 문헌 [Sambrook, J., Fritsh, E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring harbor Laboratory, Cold Spring harbor Laboratory Press, Cold Spring harbor, NY, 1989]에 기재된 바와 같은 혼성화 기술)을 이용하여 씨. 글루타미쿰 뱅크 (bank)로부터 씨. 글루타미쿰 SES cDNA를 단리할 수 있다. 또한, 부록 A의 서열 (예를 들어, 이와 동일한 서열을 기초로 하여 제작된 올리고뉴클레오티드 프라이머를 사용하는 중합효소 연쇄반응에 의해 부록 A의 전체 서열 또는 그의 일부를 포함하는 핵산 분자를 단리할 수 있음)을 기초로 하여 제작된 올리고뉴클레오티드 프라이머를 사용하는 중합효소 연쇄반응에 의해 부록 A의 전체 서열 또는 그의 일부를 포함하는 핵산 분자를 단리할 수 있다. 예를 들어, mRNA는 정상 내피세포로부터 (예를 들어, 문헌[Chirgwin et al.(1979) Biochemistry 18:5294-5299]의 구아니디늄-티오시아네이트 추출 방법에 의해) 단리할 수 있고, cDNA는 역전사효소 (예를 들어, Gibco/BRL(Bethesda, MD)로부터 시판되는 몰로네이(Moloney) MLV 역전사효소; 또는 세이까가꾸 아메리카, 인크.(Seikagaku America, Inc., St. Petersburg, FL)로부터 시판되는 AMV 역전사효소)를 사용하여 제조할 수 있다. 부록 A에 기재된 뉴클레오티드 서열 중 어느 하나를 기초로 하여 중합효소 연쇄반응 증폭을 위한 합성 올리고뉴클레오티드 프라이머를 설계할 수 있다. 본 발명의 핵산은 cDNA 또는, 별법으로, 게놈 DNA를 주형으로 사용하고 표준 PCR 증폭 기술에 따라 적합한 올리고뉴클레오티드 프라이머를 사용하여 증폭시킬 수 있다. 이와 같이 증폭된 핵산을 적합한 벡터내에 클로닝하고 DNA 서열 분석에 의해 특성화할 수 있다. 또한, SES 뉴클레오티드 서열에 상응하는 올리고뉴클레오티드는 표준 합성 기술, 예를 들어, 자동 DNA 합성기를 사용하여 제조할 수 있다.
바람직한 실시양태에서, 본 발명의 단리된 핵산 분자는 부록 A에 나열된 뉴클레오티드 서열 중 하나를 포함한다.
추가의 바람직한 실시양태에서, 본 발명의 단리된 핵산은 부록 A에 기재된 뉴클레오티드 서열 중 어느 하나에 상보적인 핵산 분자 또는 그의 일부를 포함하며, 상기 핵산 분자는 부록 A에 기재된 서열 중 어느 하나와 혼성화 (안정한 이중체 (duplex)를 형성)되기에 충분할 정도로 부록 A에 기재된 뉴클레오티드 서열 중 어느 하나에 상보적이다.
한 실시양태에서, 본 발명의 핵산 분자는 부록 B의 아미노산 서열과 충분히 상동성이 있는 아미노산 서열을 포함하는 단백질 또는 그의 부분을 코딩하며, 이 단백질 또는 그의 부분은 씨. 글루타미쿰에서 DNA 복구 및 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여하는 능력을 유지시켜 준다. 본원에 사용된 바와 같이, 용어 "충분히 상동성인"이란 단백질 또는 그의 부분의 아미노산 서열이 부록 B의 아미노산 서열에 비해 동일하거나 동등한 최소 수의 아미노산 잔기(예를 들어, 부록 B의 서열 중 어느 하나의 서열에서의 하나의 아미노산 잔기와 유사한 측쇄를 갖는 아미노산 잔기)를 포함하는 단백질 또는 그의 부분에 관한 것이며, 이 단백질 또는 그의 부분은 씨. 글루타미쿰에서 DNA 복구 및 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여할 수 있다. 씨. 글루타미쿰의 유전자 안정성, 유전자 발현, 단백질 폴딩 또는 단백질 분비에 관여하는 단백질들은, 본원에 기재된 바와 같이 1종 이상의 정밀화학물질의 생산 및 분비에서 작용할 수 있다. 이러한 활성의 예는 또한 본원에 기재되어 있다. 따라서, "ha 단백질의 기능"은 1종 이상의 정밀화학물질의 수율, 생산성 및(또는) 생산 효율에 직접 또는 간접적으로 기여한다. 표 1은 SES 단백질의 예를 나타낸다.
본 발명의 SES 핵산 분자에 의해 코딩되는 단백질의 부분은 바람직하게는 SES 단백질 중 어느 하나의 생물학적으로 활성인 부분이다. 본원에 사용된 바와 같이, 용어 "ha 단백질의 생물학적으로 활성인 부분"은 씨. 글루타미쿰에서 DNA 복구 및 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여하거나 표 1에 기재된 활성 중 어느 하나를 갖는 SES 단백질의 부분, 예를 들어, 그의 도메인/모티프를 포함한다. SES 단백질 또는 그의 생물학적으로 활성인 부분이 씨. 글루타미쿰에서 DNA 복구 및 재조합, 유전 물질의 전위, 유전자 발현 (즉, 전사 또는 번역 과정), 단백질 폴딩 또는 단백질 분비 과정에 참여할 수 있는지를 결정하기 위해, 효소 활성의 분석을 수행할 수 있다. 이러한 분석 방법은, 실시예 8에 상세하게 기재된 바와 같이, 당업자에게 숙달되어 있다.
집단내에 존재할 수 있는 SES 서열의 자연 발생적인 변이체 이외에도, 당업자라면 또한 부록 A의 뉴클레오티드 서열내의 변이에 의해, 상기 SES 단백질의 기능성을 변화시키지 않고, 코딩된 SES 단백질의 아미노산 서열에서 변화를 일으킴으로써 변화를 도입할 수 있음을 알 것이다. 예를 들어, "비필수" 아미노산 잔기에서 아미노산 치환을 일으키는 뉴클레오티드 치환이 부록 A의 뉴클레오티드 서열에서 일어날 수 있다. "비필수 아미노산" 잔기가 상기 SES 단백질의 활성을 변화시키지 않으면서 SES 단백질 (부록 B) 중 어느 하나의 야생형 서열에서 변화될 수 있는 잔기인 반면 "필수" 아미노산 잔기는 SES 단백질 활성에 요구된다. 그러나, 다른 아미노산 잔기 (예를 들어, SES 활성을 갖는 도메인에서 보존되지 않거나 또는 반보존된 잔기)는 활성에 필수적이지 않을 수도 있으며, 따라서 SES 활성을 변화시키지 않으면서도 변화시킬 수 있다.
코딩되는 단백질내에 하나 이상의 아미노산의 치환, 부가 또는 결실이 도입되도록 부록 A의 뉴클레오티드 서열내에 하나 이상의 뉴클레오티드의 치환, 부가 또는 결실을 도입시킴으로써, 부록 A의 단백질 서열과 상동성인 SES 단백질을 코딩하는 단리된 핵산 분자를 생성시킬 수 있다. 부위-지정 돌연변이유발법 및 PCR-매개 돌연변이유발법과 같은 표준 기술에 의해 부록 A의 서열들 중 어느 하나에 변이를 도입할 수 있다. 예상되는 하나 이상의 비필수 아미노산 잔기에서 보존적인 아미노산 치환을 도입하는 것이 바람직하다. "보존된 아미노산 치환"이란 아미노산 잔기가 유사한 측쇄를 갖는 아미노산 잔기로 대체되는 것을 말한다. 유사한 측쇄를 갖는 아미노산 잔기의 군은 당업계에 정의되어 있다. 이러한 군에는 염기성 측쇄를 갖는 아미노산 (예를 들어, 리신, 아르기닌, 히스티딘), 산성 측쇄를 갖는 아미노산 (예를 들어, 아스파트산, 글루탐산), 비하전된 극성 측쇄를 갖는 아미노산(예를 들어, 글리신, 아스파라긴, 글루타민, 세린, 트레오닌, 티로신, 시스테인), 비극성 측쇄를 갖는 아미노산 (예를 들어, 알라닌, 발린, 루이신, 이소루이신, 프롤린, 페닐알라닌, 메티오닌, 트립토판), 베타-분지 측쇄를 갖는 아미노산 (예를 들어, 트레오닌, 발린, 이소루이신) 및 방향족 측쇄를 갖는 아미노산 (예를 들어, 티로신, 페닐알라닌, 트립토판, 히스티딘)이 포함된다. 따라서, SES 단백질에서 예상되는 비필수 아미노산 잔기는 동일한 측쇄 군의 다른 아미노산 잔기로 치환되는 것이 바람직하다. 추가의 실시양태에서, 변이는 별법으로, 예를 들어 포화 돌연변이유발법에 의해 SES 코딩 서열의 전부 또는 일부에 걸쳐서 무작위적으로 도입될 수 있으며, SES 활성을 보유하는 변이체를 확인하기 위해, 생성된 변이체를 본원에 기재된 SES 활성에 대하여 시험할 수 있다. 부록 A의 서열들 중 어느 하나의 서열을 돌연변이 유발시킨 후에 코딩된 단백질을 재조합적으로 발현시킬 수 있으며, 예를 들어 본원에 기재된 분석법을 이용하여 단백질의 활성을 결정할 수 있다 (실시예 8 참조).
B. 재조합 발현 벡터 및 숙주 세포
본 발명의 추가 측면은 벡터, 바람직하게는 SES 단백질 (또는 그의 부분)을 코딩하는 핵산을 함유하는 발현 벡터에 관한 것이다. 본원에서 사용하는 용어 "벡터"는 그에 연결된 다른 핵산을 수송할 수 있는 핵산 분자에 관한 것이다. 벡터의 한 유형인 "플라스미드"는 추가의 DNA 단편이 라이게이션될 수 있는 환형 이중-가닥 DNA 루프를 의미한다. 벡터의 다른 유형인 바이러스 벡터는 추가의 DNA 단편을 바이러스 게놈 내로 라이게이션시킬 수 있다. 특정 벡터는 도입된 숙주 세포 내에서 자가 복제될 수 있다 (예, 세균 복제 개시점을 가진 세균 벡터 및 포유류의 에피솜 벡터). 다른 벡터 (예, 포유류의 비-에피솜 벡터)는 숙주 세포 내로 도입 시에 숙주 게놈 내로 삽입되고, 이렇게 함으로써 숙주 게놈과 함께 복제된다. 또한, 특정 벡터는 기능적으로 연결된 유전자의 발현을 조절할 수 있다. 이러한 벡터는 "발현 벡터"로 불리운다. 통상적으로, DNA 재조합 기술에 사용될 수 있는 발현 벡터는 플라스미드 형태이다. 플라스미드는 가장 통상적으로 사용되는 벡터 형태이기 때문에, 본 명세서에서는 "플라스미드"와 "벡터"를 혼용할 수 있다. 그러나, 본 발명에는 유사한 기능을 제공하는 바이러스 벡터 (예를 들어, 복제 결손 레트로바이러스, 아데노바이러스 및 아데노-관련 바이러스)와 같은 다른 형태의 발현 벡터도 포함된다.
본 발명의 재조합 발현 벡터는 본 발명의 핵산을 숙주 세포 내에서 상기 핵산을 발현하기에 적합한 형태로 포함하며, 적합한 형태란 상기 재조합 발현 벡터가 발현용 숙주 세포를 기준으로 선택된 하나 이상의 조절 서열 (발현되는 핵산 서열에 기능적으로 연결됨)을 포함하는 형태를 의미한다. 재조합 발현 벡터 내에서, 용어 "기능적으로 연결된"은 관심있는 뉴클레오티드 서열이 발현 가능하도록 조절 서열(들)에 연결된 것을 의미한다 (예, 시험관내 전사/번역 시스템 내, 또는 벡터가 숙주 세포 내로 도입되는 경우 상기 숙주 세포 내). 용어 "조절 서열"이란 프로모터, 인핸서 및 기타 발현 조절 요소 (예, 폴리아데닐화 신호)를 포함하는 것이다. 이러한 조절 서열은, 예를 들어 문헌 [Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990)]에 기재되어 있다. 조절 서열은 다수의 숙주 세포 유형 내에서 뉴클레오티드 서열이 구성적으로 발현되도록 조절하는 것과 특정 숙주 세포 내에서만 뉴클레오티드 서열이 발현되도록 조절하는 것이 포함한다. 당업자는 발현 벡터 설계가 형질전환되는 숙주 세포의 선택, 원하는 단백질의 발현 정도 등과 같은 인자들에 따라 달라질 수 있다는 것을 이해한다. 본 발명의 발현 벡터는 숙주세포에 도입됨으로써 본원에 기재된 핵산에 의해 코딩되는 융합 단백질 또는 융합 펩티드를 비롯한 단백질 또는 펩티드를 생산할 수 있다 (예, SES 단백질, SES 단백질의 돌연변이형, 융합 단백질 등).
본 발명의 재조합 발현 벡터는 원핵 또는 진핵 세포에서의 SES 단백질 발현용으로 설계될 수 있다. 예를 들어, SES 유전자는 씨. 글루타미쿰과 같은 세균 세포, 곤충 세포 (배큘로바이러스 발현 벡터 사용), 효모 및 다른 진균류 세포 (문헌 [Romanos, M.A. et al. (1992) "Foreign gene expression in yeast: a review", Yeast 8:423-488; van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet ＆ L.L. Lasure, eds., p.396-428: Academic Press: San Diego; 및 van den Hondel, C.A.M.J.J. ＆ Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge] 참조), 원충류 세포 및 다세포 식물 세포 (문헌 [Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefactiens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep.:583-586] 참조) 또는 포유류 세포 내에서 발현될 수 있다. 적합한 숙주 세포는 문헌 [Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990)]에 추가로 논의되어 있다. 별법으로, 예를 들어 T7 프로모터 조절 서열 및 T7 중합효소를 사용하여 재조합 발현 벡터를 시험관 내에서 전사 및 번역시킬 수 있다.
원핵 세포 내에서는 단백질이 주로 융합 또는 비-융합 단백질 발현을 조절하는 구성적 또는 유도적 프로모터 함유 벡터를 사용하여 발현된다. 융합 벡터는 그의 코딩되는 단백질에서 (통상적으로, 재조합 단백질의 아미노 말단에서) 다수의 아미노산을 조절한다. 이러한 융합 벡터는 통상적으로 세 가지 목적, 즉 1) 재조합 단백질의 발현 증가, 2) 재조합 단백질의 가용성 증가 및 3) 친화도 정제에서 리간드로서 작용함에 의한 재조합 단백질 정제 보조를 위한 것이다. 대개 융합 발현 벡터 내에서는 융합 잔기와 재조합 단백질의 접합부에 단백질 절단 부위가 도입되어 있어서 융합 단백질을 정제한 후 융합 잔기로부터 재조합 단백질을 분리하는 것이 가능하다. 이러한 효소 및 그의 동족 인식 서열에는 인자 Xa, 트롬빈 및 엔테로키나제가 포함된다.
통상적인 융합 발현 벡터에는 pGEX (Pharmacia Biotech Inc; 문헌 [Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40] 참조), pMAL (New England Biolabs, Beverly, MA) 및 pRIT5 (Pharmacia, Piscataway, NJ)이 포함되며, 이들은 각각 글루타티온-S-트랜스퍼라제 (GST), 말토스 E 결합 단백질 또는 프로틴 A (protein A)를 표적 재조합 단백질에 융합시킨다. 한 실시양태에서는 SES 단백질의 코딩 서열을 pGEX 발현 벡터 내로 클로닝하여 N-말단 내지 C-말단에 GST-트롬빈 절단 부위-X 단백질을 포함하는 융합 단백질 코딩 벡터를 생성하였다. 상기 융합 단백질은 글루타티온-아가로스 수지를 사용하는 친화도 크로마토그래피를 통해 정제할 수 있다. GST에 융합되지 않은 재조합 SES 단백질은 융합 단백질을 트롬빈으로 절단하여 수득할 수 있다.
적합한 유도적 비-융합 대장균 발현 벡터의 예로는 pTrc (문헌 [Amann et al.,(1988) Gene 69:301-315] 참조) 및 pET 11d (문헌 [Studier et al., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89] 참조)가 있다. pTrc로부터 표적 유전자가 발현되는 것은 혼성 trp-lac 융합 프로모터로부터 숙주 RNA 중합효소에 의한 전사에 기초한다. pET 11d 벡터로부터 표적 유전자가 발현되는 것은 T7-gn10-lac 융합 프로모터로부터 동시 발현된 바이러스 RNA 중합효소 (T7 gn1)로 매개되는 전사에 기초한다. 이 바이러스 중합효소는 lacUV 5 프로모터의 전사 조절 하에 T7 gn1 유전자를 함유하는 상재 λ프로파지로부터 숙주 균주 BL21(DE3) 또는 HMS174(DE3)에 의해 공급된다. 다양한 세균의 형질전환을 위해 적절한 벡터를 선택할 수 있다.
재조합 단백질의 발현을 극대화하는 전략의 일환으로 재조합 단백질을 단백질 분해에 의해 절단하는 능력을 감소시킨 숙주 세균 내에서 상기 단백질을 발현시키는 것이 있다 (문헌 [Gottesman, S., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128] 참조). 다른 전략으로는 발현 벡터 내로 삽입되는 핵산의 핵산 서열을 변형하여 각각의 아미노산에 대한 개별적인 코돈이 발현을 위해 선택된 세균 (예를 들어, 씨. 글루타미쿰)이 선호하는 코돈이 되도록 하는 것이 있다 (문헌 [Wada et al.(1992) Nucleic Acids Res. 20:2111-2118] 참조). 이처럼 본 발명의 핵산 서열을 변형하는 것은 표준 DNA 합성 기술에 의해 수행될 수 있다.
다른 실시양태에서, SES 단백질 발현 벡터는 효모 발현 벡터이다. 효모 에스. 세레비지애 (S. Cerevisiae)의 발현 벡터의 예로는 pYepSec1 (문헌 [Baldari, et al.,(1987) Embo J.6:229-234), pMFa (문헌 [Kurjan and Herskowitz,(1982) Cell 30:933-943] 참조), pJRY88 (문헌 [Shultz et al.,(1987) Gene 54:113-123] 참조) 및 pYES2 (Invitrogen Corporation, San Diego, CA)가 포함된다. 사상 진균류와 같은 다른 진균류에 사용하기 적합한 벡터 및 벡터의 제조 방법으로는 문헌 [van den Hondel, C.A.M.J.J. ＆ Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi] 및 [Applied Molecular Genetics of Fungi, J.F. Peberdy, et al.,eds., p. 1-28, Cambridge University Press: Cambridge]에 기재된 것들이 포함된다.
별법으로, 배큘로바이러스 발현 벡터를 사용하여 본 발명의 SES 단백질을 곤충 세포 내에서 발현시킬 수 있다. 배양된 곤충 세포 (예, Sf 9 세포)에서의 단백질 발현용 배큘로 바이러스 벡터에는 pAc계 (문헌 [Smith et al. (1983) Mol. Cell Biol. 3:2156-2165] 참조) 및 pVL계 (문헌 [Lucklow and Summers (1989) Virology 170:31-39] 참조)가 포함된다.
추가의 실시양태에서는 단세포 식물 (예를 들어, 원충류) 세포 또는 고등 식물 (예, 작물 식물과 같은 종자 식물) 세포 내에서 본 발명의 SES 단백질을 발현시킬 수 있다. 식물 발현 벡터의 예로는 문헌 [Becker, D., Kemper, E., Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197] 및 [Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acid. Res. 12: 8711-8721]에 상세하게 기재된 것들이 포함된다.
추가의 실시양태에서는 포유류 발현 벡터를 사용하여 포유류 세포 내에서 본 발명의 핵산을 발현시킨다. 포유류 발현 벡터의 예로는 pCDM8 (문헌 [Seed, B. (1987) Nature 329:840] 참조) 및 pMT2PC (문헌 [Kaufman et al. (1987) EMBO J. 6:187-195] 참조)가 포함된다. 포유류 세포를 사용하는 경우, 발현 벡터의 조절 기능은 대개 바이러스 조절 요소에 의해 제공된다. 예를 들어, 통상적으로 사용되는 프로모터는 폴리오마, 아데노바이러스 2, 사이토메갈로바이러스 및 시미안 바이러스 40으로부터 유래된 것이다. 원핵 세포 및 진핵 세포에 적합한 다른 발현계는 문헌 [Chapters 16 and 17 of Sambrook, J., Fritsh, E.F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd. ed. Cold Spring harbor Laboratory, Cold Spring harbor Laboratory Press, Cold Spring harbor, NY, 1989]에서 찾을 수 있다.
다른 실시양태에서, 재조합 포유류 발현 벡터는 특정 세포 유형에서 핵산을 발현시킬 수 있는 것이 바람직하다 (예를 들어, 조직-특이적 조절 요소가 핵산을 발현시키는데 사용됨). 조직-특이적 조절 요소는 당업계에 공지되어 있다. 적합한 조직-특이적 프로모터에 대한 제한되지 않는 예로는 알부민 프로모터 (간-특이적; 문헌 [Pinkert et al. (1987) Genes Dev. 1:268-277] 참조), 림프양-특이적 프로모터 (문헌 [Calame and Eaton (1988) Adv. Immunol. 43:235-275] 참조), 특히 T 세포 수용체의 프로모터 (문헌 [Winoto and Baltimore (1989) EMBO J. 8:729-733] 참조)와 이뮤노글로불린의 프로모터 (문헌 [Banerji et al. (1983) Cell 33:729-740], 문헌 [Queen and Baltimore (1983) Cell 33:741-748] 참조), 뉴런-특이적 프로모터 (예, 뉴로필라멘트 프로모터; 문헌 [Byrne and Ruddle (1989) PNAS 86:5473-5477] 참조), 췌장-특이적 프로모터 (문헌 [Edlund et al. (1985) Science 230:912-916] 참조) 및 유선(乳腺)-특이적 프로모터 (예, 유장(乳漿) 프로모터; 미국 특허 제4,873,316호 및 유럽 출원 번호 제264,166호 참조)가 포함된다. 또한, 발생-조절 프로모터, 예를 들어 쥐의 hox 프로모터 (문헌 [Kessel and Gruss (1990) Science 249:374-379] 참조) 및 α-페토단백질 프로모터 (문헌 [Campes and Tilghman (1989) Genes Dev. 3:537-546] 참조)도 포함된다.
또한, 본 발명은 안티센스 방향으로 발현 벡터 내에서 클로닝된 본 발명의 DNA 분자를 포함하는 재조합 발현 벡터를 제공한다. 이것은 상기 DNA 분자가 SES mRNA에 대해 안티센스인 RNA 분자의 (상기 DNA 분자의 전사를 통해) 발현이 가능하도록 조절 서열에 기능적으로 연결된다는 것을 의미한다. 안티센스 방향으로 클로닝된 핵산에 기능적으로 연결되고, 다양한 세포 유형에서 안티센스 RNA 분자의 지속적인 발현을 조절하는 조절 서열을 선택할 수 있으며, 또는 예를 들어 안티센스 RNA의 구성적이고 조직-특이적인 발현, 또는 세포형 특이적인 발현을 조절하는 바이러스 프로모터 및(또는), 또는 조절 서열을 선택할 수도 있다. 안티센스 발현 벡터는 재조합 플라스미드, 파지미드 또는 약독화 바이러스의 형태일 수 있으며, 활성이 상기 벡터가 도입된 세포 유형에 의해 결정되는 매우 효율적인 조절 영역의 조절 하에서 안티센스 핵산을 생산할 수 있다. 안티센스 유전자를 사용하여 유전자 발현을 조절하는 것이 문헌 [Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews - Trends in Genetics, Vol. 1(1) 1986]에 논의되어 있다.
본 발명의 추가 측면은 본 발명의 재조합 발현 벡터가 도입된 숙주 세포에 관한 것이다. 용어 "숙주 세포" 및 "재조합 숙주 세포"는 본원에서 혼용되고 있다. 이 용어는 특정 표적 세포 뿐만 아니라 이들 세포의 자손 또는 잠재적인 자손에 관한 것이다. 세대를 지나면서 돌연변이나 환경 인자에 의해 특정한 변형이 나타날 수 있기 때문에, 이들 자손은 부모 세포와 동일하지는 않지만, 여전히 본원에서 사용하는 용어의 범위에 포함된다.
숙주 세포는 원핵 세포 또는 진핵 세포일 수 있다. 예를 들어, SES 단백질은 씨. 글루타미쿰과 같은 세균 세포, 곤충 세포, 효모 또는 포유류 세포 (예를 들어, 중국산 큰쥐 난소 (CHO) 세포 또는 COS 세포) 내에서 발현될 수 있다. 다른 적합한 숙주 세포는 당업자에게 숙달되어 있다. 본 발명의 핵산 및 단백질 분자를 위한 숙주세포로서 적합한 방식으로 사용할 수 있으며 코리네박테리움 글루타미쿰과 관련된 미생물이 표 3에 나열되어 있다.
벡터 DNA는 통상적인 형질전환 또는 형질감염 방법을 이용하여 원핵 또는 진핵 세포 내로 도입될 수 있다. 본원에서 사용된 용어 "형질전환", "형질감염", "접합" 및 "형질도입"은 외래 핵산 (예르 들어, DNA)을 숙주 세포 내에 도입하는 당업계에 공지된 다양한 기술을 나타내며, 인산 칼슘 또는 염화 칼슘 동시 침전법, DEAE-덱스트란-매개 형질감염법, 리포펙션법 (lipofection), 천연 수용법 (natural competence), 화학적 매개 전달법 또는 전기영동법이 포함된다. 숙주 세포를 형질전환 또는 형질감염시키기에 적합한 방법은 문헌 [Sambrook et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring harbor Laboratory, Cold Spring harbor Laboratory Press, Cold Spring harbor, NY, 1989)] 및 다른 실험 안내서에서 찾을 수 있다.
포유류 세포의 안정적 형질감염의 경우에는, 사용된 발현 벡터와 이용된 형질감염 기술에 따라, 단지 일부 소수 세포만이 그들의 게놈 내로 외래 DNA를 삽입한다는 것이 알려져 있다. 이들 삽입체는 통상적으로 선별 가능 표지 (예, 항생제 내성)를 코딩하는 유전자를 관심있는 유전자와 함께 숙주 세포 내로 도입시켜 확인하고 선별한다. 바람직한 선별 가능 표지에는 G418, 하이그로마이신 및 메토트렉세이트와 같은 약물 내성을 부여하는 것들이 포함된다. 선별 가능 표지를 코딩하는 핵산은 SES 단백질을 코딩하는 동일 벡터 상에서 숙주 세포 내로 도입될 수 있거나, 별도의 벡터 상에서 도입될 수도 있다. 도입된 핵산이 안정적으로 형질감염된 세포는, 예를 들어 약물 선별에 의해 확인될 수 있다 (예를 들어, 선별 가능 표지 유전자가 혼입된 세포는 생존할 것이나, 그렇지 않은 다른 세포는 사멸함).
상동성 재조합 미생물은 결실, 부가 또는 치환을 도입시킨 SES 유전자를 변형 (예를 들어, 기능적으로 붕괴시킴)시키기 위해 SES 유전자를 적어도 하나 함유하는 벡터를 제조함으로써 생성된다. 바람직하게는 상기 SES 유전자는 코리네박테리움 글루타미쿰 SES 유전자이지만, 관련 세균으로부터의 상동체 뿐만 아니라 포유류, 효모 또는 곤충 기원의 상동체일 수도 있다. 바람직한 실시양태에서, 상기 벡터는 상동성 재조합에 의해 내생성 SES 유전자가 기능적으로 파괴 (즉, 더이상 기능적 단백질을 코딩하지 않음, "녹 아웃 (knock-out)" 벡터로도 지칭함)되도록 설계된다. 별법으로, 상기 벡터는 상동성 재조합에 의해 내생성 SES 유전자가 돌연변이되거나 달리 변형되었지만 여전히 기능적 단백질을 코딩 (예를 들어, 상류 조절 영역이 변형되어 내생성 SES 단백질의 발현이 변형될 수 있음)하도록 설계된다. 상동성 재조합 벡터에는 변형된 SES 유전자 단편의 5' 및 3'의 측면에 SES 유전자의 추가적인 핵산이 자리하여 벡터에 의해 운반된 외래 SES 유전자와 미생물 내의 내생성 SES 유전자 사이에 상동성 재조합이 일어나도록 한다. 추가된 측면 SES 핵산은 내생성 유전자와 성공적으로 상동성 재조합 되기에 충분한 길이를 갖는다. 통상적으로, 수 킬로베이스의 측면 DNA (5' 및 3' 말단 둘 다)가 상기 벡터 내에 포함된다 (예를 들어, 상동성 재조합 벡터에 대해 기술하는 문헌 [Thomas, K.R., and Capecchi, M.R. (1987) Cell 51:503] 참조). 상기 벡터는 (예, 전기영동법에 의해) 미생물 내로 도입되고, 도입된 SES 유전자와 내생성 SES 유전자가 상동적으로 재조합된 세포를 공지된 방법을 이용하여 선별한다.
다른 실시양태에서, 도입된 유전자의 발현 조절이 가능한 선별 시스템을 함유하는 재조합 미생물을 제조할 수 있다. SES 유전자의 벡터 내로의 삽입은, lac 오페론의 조절하에 유도되는 결과로서, 예를 들어 IPTG의 존재하에서만 SES 유전자 발현을 가능하게 한다. 이러한 조절 시스템은 당업계에 공지되어 있다.
배양된 원핵 또는 진핵 숙주 세포와 같은 본 발명의 숙주 세포는 SES 단백질을 생산 (즉, 발현)하는데 사용할 수 있다. 또한, 본 발명은 본 발명의 숙주 세포를 이용한 SES 단백질의 생산 방법을 제공한다. 한 실시양태에서, 상기 방법은 본 발명의 (SES 단백질을 코딩하는 재조합 발현 벡터가 도입되거나, 야생형 또는 변형된 SES 단백질을 코딩하는 유전자가 게놈 내로 도입된) 숙주 세포를 SES 단백질이 생산될 때까지 적합한 배지에서 배양하는 것을 포함한다. 추가의 실시양태에서, 상기 방법은 상기 배지 또는 숙주 세포로부터 SES 단백질을 단리하는 것을 포함한다.
C. 본 발명의 용도 및 방법
본원에 기재된 핵산 분자, 단백질, 단백질 상동체, 융합 단백질, 프라이머, 벡터 및 숙주세포는 코리네박테리움 글루타미쿰 및 관련된 유기체의 동정, 코리네박테리움 글루타미쿰과 관련된 유기체의 게놈 맵핑, 코리네박테리움 글루타미쿰의 관심있는 서열의 동정 및 위치 확인, 진화 연구, 기능에 필요한 SES 단백질 부위의 결정, SES 단백질의 활성 조절, 1종 이상의 세포 막 성분의 대사 조절, 1종 이상의 화합물의 막횡단 수송 조절, 및 정밀 화학 물질과 같은 관심있는 화합물의 세포 생산 조절 등의 방법 중 하나 이상에 사용할 수 있다. 본 발명의 SES 핵산 분자는 다양한 용도를 갖는다. 첫째, 코리네박테리움 글루타미쿰 또는 그의 가까운 관련 미생물과 같은 유기체를 동정하는데 사용할 수 있다. 또한, 혼합된 미생물군에서 코리네박테리움 글루타미쿰 또는 그의 관련 유기체의 존재를 확인하는 데에 사용할 수 있다. 본 발명은 다수의 코리네박테리움 글루타미쿰 유전자의 핵산 서열을 제공한다. 단일 또는 혼합된 미생물군 배양물에서 추출한 게놈 DNA를 엄격한 조건하에서 코리네박테리움 글루타미쿰에 고유한 유전자 영역에 걸친 프로브를 사용하여 조사함으로써 상기 유기체의 존재를 확인할 수 있다. 코리네박테리움 글루타미쿰 자체는 병원성이 아니지만, 코리네박테리움 디프테리아 (Corynebacterium diphtheriae)와 같은 병원성 종과 관련되어 있다. 상기 미생물의 검출은 실질적으로 임상에서 중요하다.
또한, 본 발명의 핵산 및 단백질 분자는 게놈의 특정 영역의 표지로 기능을 한다. 이러한 기능은 게놈 맵핑 뿐만 아니라 씨. 글루타미쿰 단백질의 기능 연구에서도 유용성이 있다. 예를 들어, 씨. 글루타미쿰 게놈을 절단하여 단편을 DNA 결합 단백질과 인큐베이션시켜, 씨. 글루타미쿰의 특정 DNA 결합 단백질이 결합하는 게놈 영역을 확인할 수 있다. 단백질에 결합하는 영역은 또한 본 발명의 핵산 분자, 바람직하게는 용이하게 검출할 수 있는 표지로 조사할 수 있다. 이와 같은 게놈 단편에 대한 핵산 분자의 결합으로 씨. 글루타미쿰의 게놈 맵에 단편의 위치를 정할 수 있으며, 상이한 효소로 여러번 수행하는 경우 단백질이 결합하는 핵산 서열을 신속하고 용이하게 결정할 수 있다. 또한, 본 발명의 핵산 분자는 연관된 종의 서열과 충분한 상동성을 가질 수 있어 브레비박테리움 락토페르멘툼 (Brevibacterium lactofermentum)과 같은 연관된 세균의 게놈 맵을 작성하기 위한 표지로 사용할 수 있다.
또한, 본 발명의 SES 핵산 분자는 진화 연구 및 단백질 구조 연구에 유용하다. 본 발명의 분자가 관여하는 대사 및 수송 과정은 매우 다양한 원핵 및 진핵 세포에 의해 이용된다. 다른 유기체와 유사한 효소를 코딩하는 핵산 분자 서열과 본 발명의 핵산 분자의 서열을 비교함으로써 유기체의 진화 연관성을 평가할 수 있다. 유사하게, 이러한 비교로 서열 영역의 보존 여부를 평가할 수 있으며, 이러한 보존 영역은 효소 기능에 필수적인 단백질 영역을 결정하는데 도움이 될 수 있다. 이러한 결정 유형은 단백질 공학에 유용하며, 단백질 기능의 손실없이 유발된 돌연변이에 내성을 가질 수 있는 지를 나타낼 수 있다.
본 발명의 SES 핵산 분자를 조작하여 야생형 SES 단백질과 다르게 작용하는 SES 단백질 생산을 유도할 수 있다. 이러한 단백질은 효율 또는 활성이 향상될 수 있거나, 정상 세포보다 많은 양으로 세포에 존재할 수 있거나, 효율 또는 활성이 감소될 수도 있다.
씨. 글루타미쿰에서 DNA 복구, 재조합 또는 전위에 관여하는 단백질의 활성을 조절하는 것은 세포의 유전자 안정성에 영향을 미칠 것이다. 예를 들어, DNA 복구 메카니즘에 관여하는 단백질의 수 또는 활성을 감소시켜 유전자 에러를 교정하는 세포 능력을 감소시킬 수 있으며, 이것은 관심있는 돌연변이 (예를 들어, 정밀화학물질 생산에 관여하는 단백질을 코딩하는 유전자)가 게놈 내로 보다 용이하게 도입될 수 있도록 할 것이다. 트랜스포손의 활성 또는 수가 증가하면 게놈 내의 돌연변이 속도도 증가하여 관심있는 유전자 (예를 들어, 정밀화학물질 생산에 관여하는 단백질을 코딩하는 유전자)의 단순 복제 또는 원치않는 유전자 (예를 들어, 정밀화학물질을 분해하는 단백질을 코딩하는 유전자)의 파괴를 가능하게 한다. 반면, 트랜스포손의 수 또는 활성이 감소하거나 DNA-복구 단백질의 수 또는 활성이 증가하면 씨. 글루타미쿰의 유전자 안정성을 증가시켜 여러 세대에 걸쳐 배양하는 동안 상기 미생물에 도입된 돌연변이가 보다 잘 유지될 수 있도록 할 것이다. 이상적으로, 돌연변이를 유발시켜 균주를 제조하는 동안 하나 이상의 DNA 복구 시스템의 활성이 감소하고, 하나 이상의 트랜스포손의 활성이 증가하면 관심있는 돌연변이가 균주 내에 도입된 경우, 반대의 결과가 나타날 것이다. 이러한 조작은 유도성 리프레서의 조절 하에 하나 이상의 DNA-복구 유전자 또는 트랜스포손을 삽입하여 수행될 수 있다
씨. 글루타미쿰에서 전사 및 번역에 관여하는 단백질을 조작하여 이 미생물로부터의 정밀화학물질 생산에 직접 또는 간접적인 영향을 끼칠 수 있다. 예를 들어, 유전자를 직접 번역하거나 (예를 들어, 중합효소) 전사를 직접 조절하는 (예를 들어, 리프레서 또는 활성인자 단백질) 단백질을 조작하여 표적 유전자의 발현에 직접적인 영향을 끼칠 수 있다. 정밀화학물질의 생합성 또는 분해에 관여하는 단백질을 코딩하는 유전자의 경우, 이러한 유형의 유전자 조작은 상기 정밀화학물질의 생산에 직접적인 영향을 끼칠 것이다. 표적 유전자를 더이상 억제하지 못하도록 하는 리프레서 단백질의 돌연변이유발 또는 활성을 최적화시키는 활성인자 단백질의 돌연변이유발은 표적 유전자의 전사를 증가시킬 것이다. 표적 유전자가, 예를 들어, 정밀화학물질 생합성 유전자인 경우, 전체적인 이용가능한 상기 유전자의 전사물 수가 증가하여 상기 화학물질의 생산량도 증가할 수 있으며, 이로 인해 단백질의 양도 증가할 것이다. 표적 서열에 대한 리프레서 단백질의 양 또는 활성의 증가, 또는 표적 서열에 대한 활성인자 단백질의 양 또는 활성의 감소는, 상기 서열이 예를 들어, 정밀화학물질을 분해하는 단백질의 서열인 경우, 상기 정밀화학물질의 생산이 유사하게 증가할 것이다.
전사 및 번역에 관여하는 단백질의 조작은 정밀화학물질 생산에 간접적인 영향을 끼칠 수도 있다. 환경에 반응하여 씨. 글루타미쿰에서 전반적인 전사를 조절하는 전사 인자 (예를 들어, 시그마 인자) 또는 번역 리프레서/활성인자, 또는 대사 인자의 활성 또는 수를 조절하여 환경 또는 전사 조절로부터 전사를 분리시킬 수 있다. 이것은 대규모 발효 배양시 나타나는 바람직하지 못한 조건 (예를 들어, 고온, 낮은 산소 함유량, 높은 폐기물 수준)과 같은, 유전자 발현이 지연되거나 중단되는 조건 하에서 계속 전사를 가능하게 한다. 이러한 상황에서, 유전자 (예를 들어, 정밀화학물질 생합성 유전자)의 발현 속도를 증가시키면 적어도 세포에 비교적 많은 정밀화학물질 생합성 단백질이 존재하게 되기 때문에 전체적인 정밀화학제품의 생산 속도도 증가시킬 수 있다. 전사 및 번역 조절 변형의 원리 및 예들은, 예를 들어 문헌 [Lewin, B. (1990) Genes IV, Part 3: "Controlling procaryotic genes by transcription", Oxford Univ. Press: Oxford, pp. 213-301]에 기재되어 있다.
폴리펩티드 폴딩에 관여하는 단백질 (예를 들어, 샤페론)의 활성 또는 수를 조절하여 세포에서 전체적인 정확하에 폴딩된 분자의 생산을 증가시킬 수 있다. 이것은 두 가지 효과를 갖는다. 첫째, 잘못 폴딩되고 분해되는 단백질이 적어지기 때문에 세포에서 전체 단백질의 수가 증가하고, 둘째, 정확하게 폴딩되어 활성을 나타내는 단백질의 양이 증가한다 (예를 들어, 문헌 [Thomas, J.G., Baneyx, F. (1997) Pretein expression and purification 11(3): 289-296], [Luo, Z.H., and Hua, Z.C. (1998) Biochemistry and Molecular Biology International 46(3):471-477], [Dale, G.E., et al., (1994) Protein Engineering 7(7):925-931], [Amrein, K.E. et al., (1995) Proc. Natl. Acad. Sci. U.S.A. 92(4):1048-1052] 및 [Caspers, P., et ale (1994) Cell. Mol. BioI. 40(5):635-644)] 참조). 상기 돌연변이가 임의의 종류의 활성인 단백질의 수를 증가시킨다고 하더라도, 이들을 예를 들어 정밀화학물질 생합성 단백질의 활성 또는 양을 증가시키는 돌연변이와 추가로 커플링시키면, 이들은 정확하게 폴딩되고 활성인 관심있는 단백질의 양에 부가적인 효과를 제공할 수 있다
씨. 글루타미쿰으로부터의 폴리펩티드 분비에 관여하는 단백질을 조작하여 이 단백질의 활성 또는 수를 향상시키면 상기 미생물로부터의 단백질생성 정밀화학물질 (예를 들어 효소)의 분비를 직접 향상시킬 수 있다. 정밀화학물질이 세포 내에 잔류하는 경우보다 대규모 배양 배지로 분비되는 경우에 이들은 훨씬 쉽게 수확하여 정제할 수 있으며, 이는 분비 시스템의 조절에 의해 정밀화학물질의 생산 수율이 증가하기 때문이다. 이들 분비 단백질의 유전자 조작은 직접 1종 이상의 정밀화학물질의 생산을 향상시킬 수 있다. 첫째로, 하나 이상의 씨. 글루타미쿰 분비 시스템의 활성을 증가시키거나 감소시켜 (이 경로에 관여하는 SES 단백질 중 하나 이상을 돌연변이화시켜 조절함) 전체적인 세포로부터의 분비 속도를 증가시키거나 감소시킬 수 있다. 이들 분지 단백질 중 다수가 세포 생존에 중요한 기능을 갖는다 (예를 들어, 세포 표면 프로테아제 또는 세포 표면 수용체). 분비 경로를 변화시켜 이들 단백질을 보다 용이하게 세포 외로 수송함으로써 전체적인 세포 생존율을 증가시켜 수많은 씨. 글루타미쿰 세포가 대규모 배양 동안 정밀화학물질을 생산할 수 있도록 할 수 있다. 둘째로, 특정 세균 분비 시스템 (예를 들어, sec 시스템)은 내재성 막 단백질 (예를 들어, 체널, 포어 또는 수송 단백질)이 세포막으로 삽입되는 과정에서 실질적인 역할을 수행하는 것으로 알려져 있다. 하나 이상의 분비 경로의 단백질의 활성이 증가하면, 세포 내에 존재하는 영양소의 양이 증가하거나 세포 내 폐물질의 양이 감소하기 때문에 정밀화학물질을 생산하는 세포 능력 또한 증가한다. 하나 이상의 분비 경로의 단백질의 활성이 감소하면, 관심있는 화합물을 과다생산하도록 하는 영양소가 충분히 존재하지 않거나 폐기물이 관심있는 화합물의 생합성을 방해할 수 있다.
씨. 글루타미쿰에서 정밀 화학 물질의 수율을 증가시키기 위한 상기언급한 SES 단백질의 돌연변이 유발 전략은 한정적이지 않으며, 이러한 돌연변이 유발 전략의 변형은 당업자에게 자명할 것이다. 이러한 방법의 이용하고 본원에 개시된 메카니즘을 포함함으로써 본 발명의 핵산 및 단백질 분자를 돌연변이된 SES 핵산 및 단백질 분자를 발현하는 씨. 글루타미쿰 또는 연관 균주를 제조하기 위해 사용하여 관심있는 화합물의 수율, 생산 및(또는) 생산 효율을 향상시킬 수 있다. 상기 관심있는 화합물은 생합성 경로의 최종 산물 및 천연 대사 경로의 중간체 뿐만 아니라 씨. 글루타미쿰 대사에서는 본래 일어나지 않으나 본 발명의 코리네박테리움 글루타미쿰 균주에 의해 생산되는 분자를 포함한 코리네박테리움 글루타미쿰에 의해 생산되는 모든 산물이 될 수 있다.
또한, 본 발명은 하기 실시예에 의해 예시되나, 이에 한정되는 것으로 해석해서는 안된다. 본원에 인용된 모든 참고 문헌, 특허 출원, 특허, 상기 특허 출원에 인용된 공개된 특허 출원은 본원에 참고문헌으로 포함된 것으로 간주한다.
실시예 1: 코리네박테리움 글루타미쿰 ATCC 13032의 전체 게놈 DNA의 프렙
BHI 배지 (디프코; Difco) 중의 코리네박테리움 글루타미쿰 (ATCC 13032) 배양물을 30℃에서 격렬히 진탕시키면서 밤새 배양하였다. 세포를 원심분리하여 수확하고, 상층액을 버리고, 세포를 5 ml 완충액-I (배양물 처음 부피의 5％에 해당함-나타낸 모든 부피는 배양 부피 100 ml에 대해 계산한 값임) 중에 재현탁하였다. 완충액-I의 조성은 다음과 같았다: 140.34 g/L 수크로스, 2.46 g/L MgS04ㆍ7 H2O, 10 ml/L KH2PO4 용액 (100 g/L, KOH를 사용하여 pH 6.7로 조정), 50 ml/L M12 농축물 (10 g/L (NH4)2SO4, 1 g/L NaCl, 2 g/L MgSO4ㆍ7 H2
O, 0.2 g/L CaCl2, 0.5 g/L 효모 추출물 (디프코), 10 ml/L 미량 원소 혼합물 (200 mg/L FeSO4ㆍH2O, 10 mg/L ZnSO4ㆍ7 H2O, 3 mg/L MnCl2ㆍ4 H2O, 30 mg/L H3BO
3, 20 mg/L CoCl2ㆍ6 H2O, 1 mg/L NiCl2ㆍ6 H2O, 3 mg/L Na2MoO4ㆍ2 H2O, 500 mg/L 착화제 (EDTA 또는 시트르산), 100 ml/L 비타민 혼합물 (0.2 mg/L 바이오틴, 0.2 mg/L 엽산, 20 mg/L p-아미노 벤조산, 20 mg/L 리보플라빈, 40 mg/L ca-판토테네이트, 140 mg/L 니코틴산, 40 mg/L 피리독솔 히드로클로라이드, 200 mg/L 미오-이노시톨). 상기 현탁액에 리소자임을 최종 농도 2.5 mg/ml로 첨가하였다. 37℃에서 대략 4 시간 동안 인큐베이션시킨 다음, 세포벽을 파괴하고, 수득한 원형질체를 원심분리에 의해 수확하였다. 펠렛을 5 ml 완충액-I으로 1 회 세척하고, 5 ml TE-완충액 (10 mM 트리스-HCl, 1 mM EDTA, pH 8)으로 1 회 세척하였다. 펠렛을 4 ml TE-완충액 중에 재현탁하고, 0.5 ml SDS 용액 (10％) 및 0.5 ml NaCl 용액 (5 M)을 첨가하였다. 프로테이나제 K를 최종 농도 200 ㎍/ml로 첨가한 다음, 상기 현탁액을 37℃에서 대략 18 시간 동안 인큐베이션하였다. 표준 방법을 이용하여, 페놀, 페놀/클로로포름/이소아밀 알콜 및 클로로포름/이소아밀 알콜로 추출하여 DNA를 정제하였다. 그 다음, 1/50 부피의 3 M 아세트산 나트륨 및 2 배 부피의 에탄올을 첨가하여 DNA를 침전시킨 후, -20℃에서 30 분 동안 인큐베이션시키고, SS34 로터 (소르발; Sorvall)를 이용하는 고속 원심분리기로 12,000 rpm에서 30 분 동안 원심분리하였다. 상기 DNA를 20 ㎍/ml RNaseA를 함유한 1 ml TE-완충액 중에 용해시키고, 4℃에서 3 시간 이상 동안 1000 ml TE-완충액에 대해 투석하였다. 상기 투석 과정 동안, 상기 완충액을 3 회 교환하였다. 투석된 DNA 용액의 0.4 ml 분취액에 2 M LiCl 0.4 ml 및 에탄올 0.8 ml를 첨가하였다. -20℃에서 30 분 동안 인큐베이션시킨 다음, 원심분리 (13,000 rpm, 독일 하나우 헤래우스 소재의 바이오퓨즈 프레스코 (Biofuge Fresco))하여 DNA를 수집하였다. DNA 펠렛을 TE-완충액 중에 용해시켰다. 상기 방법으로 프렙된 DNA를 서던 블럿팅 및 게놈 뱅크 제작을 비롯한 모든 목적에 이용할 수 있었다.
실시예 2: 대장균에서 코리네박테리움 글루타미쿰 ATCC13032의 게놈 뱅크 제작
실시예 1에 기재한 바와 같이 프렙된 DNA로부터 출발하여, 공지되고 수립된 방법 (예를 들면, 문헌 [Sambrook, J. et al. (1989) "Molecular Cloning: A Laboratory Manual", Cold Spring harbor Laboratory Press] 또는 문헌 [Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley ＆ Sons] 참조)에 따라 코스미드 및 플라스미드 뱅크를 제작하였다.
임의의 플라스미드 또는 코스미드를 사용할 수 있었다. 특히, 플라스미드 pBR322 (문헌 [Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741]); pACYC177 (문헌 [Change ＆ Cohen (1978) J. Bacteriol 134:1141-1156]), pBS 계열의 플라스미드 (pBSSK+, pBSSK- 등; 문헌 [Stratagene, LaJolla, USA]), 또는 SuperCos1 (스트라타진 (Stratagene), 라졸라 (LaJolla), USA) 또는 Lorist6 (문헌 [Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286])과 같은 코스미드를 사용하는 것이 바람직하였다.
실시예 3: DNA 서열분석 및 컴퓨터를 이용한 기능 분석
실시예 2에 기재한 바와 같은 게놈 뱅크를, 표준 방법에 따른 DNA 서열분석, 특히 ABI377 서열분석기를 이용하는 쇄 종결 방법 (예를 들면, 문헌 [Fleischman, R.D. et al. (1995) "Whole-genome Random Sequencing and Assembly of haemophilus Influenzae Rd., Science, 269:496-512)에 따른 DNA 서열분석에 사용하였다. 다음의 뉴클레오티드 서열을 갖는 서열분석 프라이머를 사용하였다: 5'-GGAAACAGTATGACCATG-3' 또는 5'-GTAAAACGACGGCCAGT-3'.
실시예 4: 생체내 돌연변이유발법
코리네박테리움 글루타미쿰의 생체내 돌연변이유발법은 완전한 유전 정보를 유지할 수 없는 손상된 대장균 또는 다른 미생물 (예를 들면, 바실러스 종 (Bacillus spp.) 또는 사카로마이세스 세레비지애 (Saccharomyces cerevisiae)와 같은 효모)에 플라스미드 (또는 다른 벡터) DNA를 계대접종하여 수행할 수 있다. 통상적인 돌연변이유발 균주는 DNA 복구 시스템에 관한 유전자 (예를 들면, mutHLS, mutD, mutT 등; 비교를 위해, 문헌 [Rupp, W.D. (1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p.2277-2294, ASM: Washington]을 참조함)에서 돌연변이를 함유한다. 이러한 균주들은 당업자에게 공지되어 있다. 이런 균주의 용도는 예를 들어, 문헌 [Greener, A. and Callahan, M. (1994) Strategies 7:32-34]에 설명되어 있다.
실시예 5: 대장균과 코리네박테리움 글루타미쿰 사이의 DNA 전달
여러 코리네박테리움 및 브레비박테리움 종은 자율적으로 복제되는 내생성 플라스미드 (예를 들어, pHM1519 또는 pBL1)를 함유하고 있다 (이에 관해 살펴보기 위해서는, 예를 들어 문헌 [Martin, J.F. et al. (1987) Biotechnology, 5:137-146]을 참조한다). 대장균 및 코리네박테리움 글루타미쿰에 사용하기 위한 셔틀 벡터는 코리네박테리움 글루타미쿰을 위한 복제 기점 및 코리네박테리움 글루타미쿰으로부터의 적합한 마커를 첨가한, 대장균에 사용하는 표준 벡터 (문헌 [Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring harbor Laboratory Press] 또는 문헌 [Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley ＆ Sons])를 사용하여 용이하게 제작할 수 있다. 상기 복제 기점은 코리네박테리움 및 브레비박테리움 종으로부터 단리한 내생성 플라스미드로부터 얻는 것이 바람직하다. 특히, 상기 종에 관한 형질전환 표지로 사용하는 유전자는 카나마이신 내성 유전자 (예를 들면, Tn5 또는 Tn903 트랜스포손으로부터 유래함) 또는 클로람페니콜 내성 유전자 (문헌 [Winnacker, E.L. (1987) "From Genes to Clones-Introduction to Gene Technology, VCH, Weinheim])이다. 대장균 및 씨. 글루타미쿰에서 복제되고, 유전자 과다발현을 비롯한 여러 목적에 사용할 수 있는 매우 다양한 셔틀 벡터의 제작법에 관한 수많은 문헌이 있다 (예를 들어 문헌 [Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597], 문헌 [Martin J.F. et al. (1987) Biotechnology, 5:137-146] 및 문헌 [Eikmanns, B.J. et al. (1991) Gene, 102:93-98] 참조).
표준 방법을 사용하여, 관심있는 유전자를 상기 기재된 셔틀 벡터들 중 하나에 클로닝하고 이러한 하이브리드 벡터를 코리네박테리움 글루타미쿰 균주에 도입할 수 있다. 코리네박테리움 글루타미쿰의 형질전환은 원형질체 형질전환법 (문헌 [Kastsumata, R. et al. (1984) J. Bacteriol. 159:306-311]), 전기영동법 (문헌 [Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303]) 및 특정 벡터가 사용되는 경우에는 접합법 (예를 들면, 문헌 [Schaefer, A et al. (1990) J. Bacteriol. 172:1663-1666]에 기재된 바와 같음)에 의해서도 달성될 수 있다. 또한, 코리네박테리움 글루타미쿰으로부터 플라스미드 DNA를 프렙 (당업계에 공지된 표준 방법을 사용)하고, 이것을 대장균으로 형질전환시켜 코리네박테리움 글루타미쿰에 사용하기 위한 셔틀 벡터를 대장균에 전달할 수도 있다. 이러한 형질전환 단계는 표준 방법을 사용하여 수행할 수 있지만, NM522 (문헌 [Gough ＆ Murray (1983) J. Mol. Biol. 166:1-19]) 등과 같은 Mcr-결핍 대장균 균주를 사용하는 것이 유리하다.
실시예 6: 돌연변이 단백질의 발현 평가
형질전환된 숙주 세포에서 돌연변이된 단백질의 활성을 관찰한 결과, 상기 돌연변이 단백질이 야생형 단백질과 유사한 방식 및 유사한 양으로 발현된다는 사실을 알아냈다. 돌연변이 유전자의 전사량 (유전자 산물의 번역에 이용가능한 mRNA 양에 관한 지표)을 확인하는 데 적합한 방법은, 노던 블럿팅 (참고를 위해서는, 예를 들어 문헌 [Ausubel et al. (1988) Current Protocols in Molecular Bioloy, Wiley: New York]을 참조한다)을 수행하는 것이며, 이는 생물 배양물의 전체 RNA를 추출하여 겔상에서 전개시키고, 이를 안정한 매트릭스로 전달한 후, 관심 유전자에 결합하도록 고안하여 검출가능한 표지 (통상적으로 방사성 또는 화학발광성)로 표지한 프라이머와 인큐베이션시켜, 상기 프로브의 결합 및 그 결합량이 상기 관심있는 유전자의 mRNA 존재 여부 및 양을 또한 지시하는 방법이다. 이러한 정보는 돌연변이 유전자의 전사 정도의 지표이다. 세포내 전체 RNA는 코리네박테리움 글루타미쿰으로부터 문헌 [Bormann, E.R. et al. (1992) Mol. Microbiol. 6:317-326]에 기재된 것과 같이 당업계에 공지된 여러 방법에 의해 단리될 수 있다.
상기 mRNA로부터 번역된 단백질의 존재 여부 또는 그 상대량은 웨스턴 블럿팅과 같은 표준 기술을 사용하여 평가할 수 있다 (예를 들어, 문헌 [Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York]을 참조). 이 방법에서, 세포내 전체 단백질을 추출하고, 겔 전기영동법으로 분획한 후, 이를 니트로셀룰로스와 같은 매트릭스로 전달하여, 관심있는 단백질에 특이적으로 결합하는 프로브 (예를 들어, 항체)와 인큐베이션하였다. 통상적으로, 이 프로브는 용이하게 검출될 수 있는 화학발광성 또는 비색성 표지로 제공되었다. 표지의 존재 여부 및 관찰된 양은 그 세포 내에서 원하는 돌연변이 단백질의 존재 여부 및 양을 나타내었다.
실시예 7: 유전적으로 변형된 코리네박테리움 글루타미쿰의 성장 - 배지 및 배양 조건
유전적으로 변형된 코리네박테리아를 합성 또는 천연 성장 배지에서 배양하였다. 다수의 상이한 코리네박테리아용 성장 배지는 공지되어 있으며, 쉽게 이용할 수 있었다 (문헌 [Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32:205-210], 문헌 [von der Osten et al. (1998) Biotechnology Letters, 11:11-16], 문헌 [독일 특허 제4,120,867호], 문헌 [Liebl (1992) "The Genus Corynebacterium", in: The Procaryotes, Volume II, Balows, A. et al., eds. Springer-Verlag]). 상기 배지는 1 종 이상의 탄소 공급원, 질소 공급원, 무기염, 비타민 및 미량 원소로 구성된다. 바람직한 탄소 공급원은 단당류, 이당류, 또는 다당류 등의 당이다. 예를 들면, 글루코스, 프룩토스, 만노스, 갈락토스, 리보스, 소르보스, 리불로스, 락토스, 말토스, 수크로스, 라피노스, 전분 또는 셀룰로스가 매우 훌륭한 탄소 공급원으로 기능한다. 또한, 당 정제시 생성되는 당밀 또는 기타 부산물 등과 같은 복합 화합물 등을 통해 당을 배지에 공급할 수도 있다. 또한, 여러 탄소 공급원들의 혼합물을 첨가하는 것도 유리할 수 있다. 다른 가능한 탄소 공급원은 메탄올, 에탄올, 아세트산 또는 락트산과 같은 알콜 및 유기산이다. 질소 공급원은 통상적으로 유기 또는 무기 질소 화합물, 또는 이들 화합물을 함유하는 물질이다. 질소 공급원의 예로는 암모니아 기체 또는 NH4Cl 또는 (NH4)2SO4, NH40H 등의 암모늄염, 질산염, 우레아, 아미노산 또는 옥수수 침유 (steep liquor), 대두 가루, 대두 단백질, 효모 추출물, 육류 추출물과 같은 복합 질소 공급원 등이 있다.
배지에 포함될 수 있는 무기 염 화합물로는 칼슘, 마그네슘, 나트륨, 코발트, 몰리브데늄, 칼륨, 망간, 아연, 구리 및 철의 염산염, 인산염, 또는 황산염이 있다. 금속 이온을 용액 상태로 유지하기 위해 킬레이트제를 배지에 첨가할 수 있다. 특히 적합한 킬레이트제로는 카테콜 또는 프로토카테쿠에이트와 같은 디히드록시페놀, 및 시트르산과 같은 유기산이 있다. 또한, 통상적으로 배지에는 비타민 또는 성장 촉진제 등의 다른 성장 인자가 포함되어 있고, 이들의 예로는 비오틴, 리보플라빈, 티아민, 엽산, 니코틴산, 판토페네이트 및 피리독신 등이 있다. 성장 요소 및 염은 흔히 효모 추출물, 당밀, 옥수수 침유 등과 같은 복합 배지 성분으로부터 유래한다. 배지 화합물의 정확한 조성은 특정 실험에 따라 크게 달라지고, 각각의 특정 경우마다 따로 결정된다. 배지의 최적화에 관한 정보는 문헌 ["Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp.53-73, ISBN 0 19 963577 3)]에서 찾을 수 있다. 또한, 성장 배지는 상업 공급자들로부터 수득할 수 있다 (예를 들어, 스탠다드 1 (머크; Merck) 또는 BHI (곡물 속 침출물, 디프코).
모든 배지 성분들을 열처리 (1.5 bar 및 121℃에서 20 분) 또는 멸균 여과로 멸균시킨다. 이 성분들을 한꺼번에 멸균하거나, 필요하다면 개별적으로 멸균할 수 있다. 모든 배지 성분들은 배양 초기에 존재할 수 있거나, 바람직하다면 연속적으로 또는 배치식으로 (batchwise) 첨가될 수 있다.
배양 조건은 각 실험마다 개별적으로 정의된다. 온도는 15℃와 45℃ 사이의 범위여야 하며, 온도는 일정하게 유지하거나 실험하는 동안 변경할 수 있다. 배지의 pH는 5 내지 8.5의 범위, 바람직하게는 약 7.0이어야 하며, 배지에 완충액을 첨가하여 유지시킬 수 있다. 이러한 목적으로 사용할 수 있는 완충액의 예로는 인산 칼륨 완충액 등이 있다. MOPS, HEPES, ACES 등과 같은 합성 완충액을 대안적으로 사용하거나 동시에 사용할 수 있다. 또한, 배양하는 동안 NaOH 또는 NH4OH를 첨가하여 배양물의 pH를 일정하게 유지시킬 수도 있다. 효모 추출물과 같은 복합 배지 성분이 사용되는 경우, 다수의 복합 화합물들은 높은 완충 성능을 갖기 때문에, 추가의 완충액에 대한 요구를 감소시킬 수 있다. 미생물 배양용 발효기를 사용하는 경우, pH는 암모니아 기체를 사용하여 조절할 수도 있다.
인큐베이션 기간은 통상적으로 수 시간 내지 수 일 범위이다. 이 시간은 브로스 (broth)에 축적되는 생성물의 양이 최대가 되도록 선택하였다. 개시한 증식 실험을 상이한 크기의 마이크로타이터 플레이트, 유리관, 유리 플라스크 또는 유리 또는 금속 발효기와 같은 다양한 용기에서 수행할 수 있다. 수많은 클론을 스크리닝하기 위해서는, 미생물을 배플 (baffle)이 있거나 없는 마이크로타이터 플레이트, 유리관 또는 진탕 플라스크에서 배양해야 한다. 필요한 증식 배지 부피의 10 ％의 양으로 채운 100 ml 진탕 플라스크를 사용하는 것이 바람직하다. 이 플라스크들은 100-300 rpm범위의 속도에서 회전 진탕기 상에서 진탕 (진폭 25 mm)되어야 한다. 증발에 의한 손실량은 대기를 습하게 유지함으로써 감소될 수 있고, 별법으로, 증발에 의한 손실량을 수학적으로 보정해야 한다.
유전적으로 변형된 클론을 조사하는 경우, 비변형된 대조 클론 또는 인서트가 없는 기본 플라스미드를 함유하는 대조 클론도 분석해야 한다. 30℃에서 인큐베이션한 CM 플레이트 (10 g/L 글루코스, 2.5 g/L NaCl, 2 g/L 우레아, 10 g/L 폴리펩톤, 5 g/L 효모 추출물, 5 g/L 육류 추출물, 22 g/L 아가, 2 M NaOH를 사용하여 pH 6.8로 조정)와 같은 아가 플레이트 상에서 증식시킨 세포를 OD600 0.5-1.5이 되도록 배지에 접종하였다. 배지 접종은 CM 플레이트로부터의 씨. 글루타미쿰 세포의 염수 현탁액을 도입하거나, 상기 세균의 액체 예비배양물을 첨가하여 수행하였다.
실시예 8: 돌연변이 단백질의 기능에 관한 시험관내 분석
효소의 활성 및 반응 속도 파라미터의 측정법은 당업계에 공지되어 있다. 특정 변형된 효소의 활성을 측정하는 실험은 야생형 효소의 비활성(比活性)에 맞추어야 하며, 당업자는 이를 잘 수행할 수 있다. 효소의 구조, 반응 속도, 원리, 방법, 적용에 관한 구체적인 세부사항 뿐만 아니라 효소에 관한 일반적인 개요 및 많은 효소들의 활성 측정 방법의 예를 들어 하기의 참고문헌에서 찾을 수 있다: 문헌 [Dixon, M., and Webb, E.C., (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism. Freeman: New York], 문헌 [Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: SanFrancisco], 문헌 [Price, N.C., Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ. Press: Oxford], 문헌 [Boyer, P.D., ed. (1983) The Enzymes, 3rd ed. Academic Press: New York], 문헌 [Bisswanger, H., (1994) Enzymkinetik, 2nd ed. VCH: Weinheim (ISBN 3527300325)], 문헌 [Bergmeyer, H.U., Bergmeyer, J., Grassl, M., eds. (1983-1986) Methods of Enzymatic Analysis, 3rd ed., vol.I-XII, Verlag Chemie: Weinheim], 및 문헌 [Ullmann's Encyclopedia of Industrial Chemistry (1987) vol.A9, "Enzymes". VCH: Weinheim, p.352-363].
DNA에 결합하는 단백질 활성은 DNA 밴드-변위 분석법 (겔 지연 분석법 (gel retardation assays)으로도 지칭됨)과 같은 잘 수립된 여러 방법에 의해 측정될 수 있다. 상기 단백질들의 다른 분자들의 발현에 대한 작용은 리포터 유전자 분석법 (문헌 [Kolmar, H. et al. (1995) EMBO J. 14:3895-3904] 및 이 문헌에서 인용한 참고 문헌에 기재된 바와 같음)을 사용하여 측정할 수 있다. 베타-갈락토시다제와 같은 효소, 녹색 형광 단백질 및 여러 기타 효소를 사용하는 리포터 유전자 시험 시스템은 원핵 세포 및 진핵 세포에의 적용에 대해 공지되어 있으며, 잘 수립되어 있다.
막 수송 단백질의 활성은 문헌 [Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p.85-137, 199-234, 및 270-322]에 기재된 기술 등에 따라 측정될 수 있다.
실시예 9: 관심있는 생성물 생산에 대한 돌연변이 단백질의 영향 분석
코리네박테리움 글루타미쿰에서의 유전적 변형이 원하는 화합물 (예를 들면, 아미노산)의 생산에 미치는 영향은 변형된 미생물을 적당한 조건 (예를 들면, 상기 기재한 바와 같음) 하에 배양하고, 원하는 생성물 (즉, 아미노산)의 생성 증가에 관여한 배지 성분 및(또는) 세포내 성분을 분석하여 평가할 수 있다. 이러한 분석 기술은 당업자에게 공지되어 있으며, 분광분석법, 박층크로마토그래피법, 각종 염색법, 효소적 및 미생물학적 방법, 및 고성능 액체 크로마토그래피법 등과 같은 분석용 크로마토그래피법 등이 있다 (예를 들면, 문헌 [Ullman, Encyclopedia of Industrial Chemistry, vol.A2, p.89-90 및 p.443-613, VCH: Weinheim (1985)], 문헌 [Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and Molecular Biology, vol.17], 문헌 [Rehm et al. (1993) Biotechnology, vol.3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim], 문헌 [Belter, P.A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons], 문헌 [Kennedy, J.F. and Cabral, J.M.S. (1992) Recovery procesha for biological materials, John Wiley and Sons], 문헌 [Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of Industrial Chemistry, vol.B3, Chapter 11, page 1-27, VCH: Weinheim], 및 문헌 [Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications] 참조).
최종 발효 생성물의 측정 뿐만 아니라, 전체적인 화합물 생성 효율의 측정하기 위해 중간체 및 부산물과 같은 관심있는 화합물의 생성에 이용된 대사 경로 중의 다른 성분들의 분석할 수 있다. 분석 방법으로는 배지 내의 영양분 (예를 들면, 당, 탄화수소, 질소 공급원, 인산염 및 기타 이온) 함량 측정, 생물집단 (biomass)의 조성 및 증식량 측정, 생합성 경로의 공통 대사물질의 생성 분석, 및 발효 동안에 생성된 기체 측정 등이 있다. 이러한 측정을 위한 표준 방법은 문헌 [Applied Microbial Physiology, A Practical Approach, P.M.Rhodes and P.F.Stanbury, eds., IRL Press, p.103-129, 131-163, 및 165-192 (ISBN:0199635773) 및 이 문헌에서 인용한 참고 문헌]에 약술되어 있다.
실시예 10: 씨. 글루타미쿰 배양물로부터의 관심있는 생성물 정제
관심있는 생성물은 씨. 글루타미쿰 세포 또는 상기 기재한 배양물의 상층액으로부터 당업계에 공지된 여러 방법을 사용하여 수득할 수 있다. 관심있는 생성물이 세포에 의해 분비되지 않는 경우, 배양물을 저속 원심분리하여 세포를 수거하고, 기계적인 힘 또는 초음파 등의 표준 기술로 세포를 용균시킬 수 있다. 원심분리로 세포 부스러기를 제거하고, 가용성 단백질을 함유하는 상층액 부분은 남겨 원하는 화합물의 추가의 정제에 사용하였다. 코리네박테리움 글루타미쿰 세포로부터 생성물이 분비되는 경우, 배양물을 저속 원심분리하여 세포를 제거하고, 상층액 부분은 남겨 추가의 정제에 사용하였다.
상기 두 가지 정제 방법 중 임의의 방법에 의한 상층액 부분을 적당한 수지를 사용한 크로마토그래피에 적용하여, 원하는 분자는 크로마토그래피 수지 상에 남지만 샘플 중의 많은 불순물은 남지 않게 하거나, 불순물들은 수지에 남지만 원하는 분자는 남지 않게 하였다. 필요에 따라, 동일하거나 상이한 크로마토그래피 수지를 사용하여 이러한 크로마토그래피 단계를 반복할 수 있다. 당업자는 적합한 크로마토그래피 수지를 선택하고 특정 분자의 정제에 대해 가장 효과적으로 이를 적용하는 것에 숙달되어 있을 것이다. 정제된 생성물을 여과법 또는 한외여과법으로 농축시켜, 생성물의 안정성이 최대가 되는 온도에 보관할 수 있다.
당업계에 공지된 정제 방법은 다양하며, 상기 기재한 정제법에 제한되지 않는다. 이러한 정제 기술은 예를 들어 문헌 [Bailey, J.E. ＆ Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986)]에 기재되어 있다.
단리된 화합물의 확인 및 순도는 당업계의 표준 기술로 측정될 수 있다. 이들 기술에는 고성능 액체 크로마토그래피 (HPLC)법, 분광분석법, 염색법, 박층 크로마토그래피법, NIRS, 효소적 분석법, 또는 미생물학적 방법이 있다. 상기 분석 방법은 하기 문헌에서 검토된다: 문헌 [Patek et al. (1994) Appl. Environ. Microbiol. 60:133-140], 문헌 [Malakhova et al. (1996) Biotekhnologiya 11:27-32], 문헌 [Schmidt et al. (1998) Bioprocess Engineer. 19:67-70], 문헌 [Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol.A27, VCH: Weinheim, p.89-90, p.521-540, p.540547, p.559-566, p.575-581 및 p.581-587], 문헌 [Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons], 및 문헌 [Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol.17].
동등물
당업자는 간단하게 통상적인 방법을 이용하여 다수의 본 발명의 구체적인 실시양태 동등물을 인지하거나 확인할 수 있다. 이들 동등물을 하기 특허 청구항에 포함시킬 것이다.
표 1의 정보는 하기와 같이 이해된다:
컬럼 1 "DNA ID"에서, 관련 번호는 각각의 경우에 동봉된 서열 목록의 서열 번호를 나타낸다. 따라서, 컬럼 "DNA ID"에서 "5"는 서열 번호 5를 나타낸다.
컬럼 2 "AA ID"에서, 관련 번호는 각각의 경우에 동봉된 서열 목록의 서열 번호를 나타낸다. 따라서, 컬럼 "AA ID"에서 "6"은 서열 번호 6을 나타낸다.
컬럼 3 "식별 명칭"에는, 각각의 서열에 대한 명백한 내부 명칭이 나열되어 있다.
컬럼 4 "AA pos"에서, 관련 번호는 각각의 경우에 동일한 순서로 폴리펩티드 서열 "AA ID"의 아미노산 위치를 나타낸다. 따라서, 컬럼 "AA pos"에서 "26"은 적절하게 표시한 폴리펩티드 서열 중에서 26번째에 위치하는 아미노산이다.
컬럼 5 "AA 야생형"에서, 관련 문자는 각각의 경우에 컬럼 4에 표시한 상응하는 야생형 균주에서의 위치에서 단일-문자 코드로 표현한 아미노산을 나타낸다.
컬럼 6 "AA 돌연변이"에서, 관련 문자는 각각의 경우에 컬럼 4에 표시한 상응하는 돌연변이 균주에서의 위치에서 단일-문자 코드로 표현한 아미노산을 나타낸다.
컬럼 7 "기능"에는, 상응하는 폴리펩티드 서열의 생리학적 기능이 나열되어 있다.
단백질생성 아미노산의 단일-문자 코드:
A 알라닌
C 시스테인
D 아스파트산
E 글루탐산
F 페닐알라닌
G 글리신
H 히스티딘
I 이소루이신
K 리신
L 루이신
M 메티오닌
N 아스파라긴
P 프롤린
Q 글루타민
R 아기닌
S 세린
T 트레오닌
V 발린
W 트립토판
Y 티로신
SEQUENCE LISTING
<110> BASF Aktiengesellschaft
<120> Genes coding for proteins for genetic stability, gene expression
and folding
<130> O.Z. 0050/52974
<160> 104
<210> 1
<211> 1990
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1960)
<223> RXA00019
<400> 1
accggatacc ttatgaaaca cctggtgagc ggtgtgtttc accccaacaa ccgagtaaaa 60
tatatctagt actattttac gattgaaagt agatttttct atg acc gtt acc tca 115
Met Thr Val Thr Ser
1 5
cca gca gcg ctc gca ctc agc gac atg tcc tat gtg gac atc att aag 163
Pro Ala Ala Leu Ala Leu Ser Asp Met Ser Tyr Val Asp Ile Ile Lys
10 15 20
aag aag cgc gga tgg aca acc gag ttt ttc cac agc acc atc aac acc 211
Lys Lys Arg Gly Trp Thr Thr Glu Phe Phe His Ser Thr Ile Asn Thr
25 30 35
ggt gaa acc acc aca ccg cta cca gac agc gac cgt gcc aca gca cta 259
Gly Glu Thr Thr Thr Pro Leu Pro Asp Ser Asp Arg Ala Thr Ala Leu
40 45 50
atc cat gac cac atc acc aag gct caa gag ata acc atc atc acc gac 307
Ile His Asp His Ile Thr Lys Ala Gln Glu Ile Thr Ile Ile Thr Asp
55 60 65
ttt gat atg gac ggt att tca gcc ggt gtc att gcc tat gca ggt ctt 355
Phe Asp Met Asp Gly Ile Ser Ala Gly Val Ile Ala Tyr Ala Gly Leu
70 75 80 85
gcc gaa ctg ggc gca cag gtc aat atg gtg gtg ccc gac tat cgt ggc 403
Ala Glu Leu Gly Ala Gln Val Asn Met Val Val Pro Asp Tyr Arg Gly
90 95 100
gaa cga aat gtc aca gcc agc gat att gat cgt gcg cta gag ctc tac 451
Glu Arg Asn Val Thr Ala Ser Asp Ile Asp Arg Ala Leu Glu Leu Tyr
105 110 115
cct gca acc tca ctc atc atc acc tgc gat gtc ggc atc ggc tcc cat 499
Pro Ala Thr Ser Leu Ile Ile Thr Cys Asp Val Gly Ile Gly Ser His
120 125 130
gaa ggt att gcc cgt gct cac gaa cgc agt atc gcc gtc ctg gtc aca 547
Glu Gly Ile Ala Arg Ala His Glu Arg Ser Ile Ala Val Leu Val Thr
135 140 145
gat cac cac atg gag gtc gaa ccc tgc cag gcc gat gtg gtt ctt aac 595
Asp His His Met Glu Val Glu Pro Cys Gln Ala Asp Val Val Leu Asn
150 155 160 165
ccc aac aga att gac tct gac tac ccc aac aaa gat att tgc ggt gcg 643
Pro Asn Arg Ile Asp Ser Asp Tyr Pro Asn Lys Asp Ile Cys Gly Ala
170 175 180
cag gtc att ttc gcc aca ttg agt gac tat gca cgt cgt tat cgg gcg 691
Gln Val Ile Phe Ala Thr Leu Ser Asp Tyr Ala Arg Arg Tyr Arg Ala
185 190 195
gac aag att atc gac att aat ttg ttg gct gtt ttc tca ggc att ggt 739
Asp Lys Ile Ile Asp Ile Asn Leu Leu Ala Val Phe Ser Gly Ile Gly
200 205 210
gca ctc gcc gat gtc atg cct ctc acc cgt gac act cga cca aca gtg 787
Ala Leu Ala Asp Val Met Pro Leu Thr Arg Asp Thr Arg Pro Thr Val
215 220 225
aag cag gct att gcg ttg ctt cgg ctt gct atc cca caa gta agt aaa 835
Lys Gln Ala Ile Ala Leu Leu Arg Leu Ala Ile Pro Gln Val Ser Lys
230 235 240 245
aac cgt ttc ggc ggt tgg gat acc tat gct gca cgc tct gtt aat cct 883
Asn Arg Phe Gly Gly Trp Asp Thr Tyr Ala Ala Arg Ser Val Asn Pro
250 255 260
gat acg tcc aca ctc atg cat att gtc aat gcc agc cag cat gat cac 931
Asp Thr Ser Thr Leu Met His Ile Val Asn Ala Ser Gln His Asp His
265 270 275
cgc ttc att gca gcc ttc caa ggc atc tca att ctt ctt ggt gaa ctg 979
Arg Phe Ile Ala Ala Phe Gln Gly Ile Ser Ile Leu Leu Gly Glu Leu
280 285 290
att gcg caa aag aag cta gta aac atc gac aat att tct gag tca ttc 1027
Ile Ala Gln Lys Lys Leu Val Asn Ile Asp Asn Ile Ser Glu Ser Phe
295 300 305
att ggc ttc act ctt ggt ccg atg ttt aac gct act cgt cgt gtt ggt 1075
Ile Gly Phe Thr Leu Gly Pro Met Phe Asn Ala Thr Arg Arg Val Gly
310 315 320 325
ggc gac atg cac gat tca ttt ctc gtg ttt gcg ccc cat gcc gca cta 1123
Gly Asp Met His Asp Ser Phe Leu Val Phe Ala Pro His Ala Ala Leu
330 335 340
gca tca cag ccg tcg atg aat cca aat cga cat gct gcg atc tct cgc 1171
Ala Ser Gln Pro Ser Met Asn Pro Asn Arg His Ala Ala Ile Ser Arg
345 350 355
atc att gat aac aac gaa cgt cgc aaa gag ctc tcc aag tcc tct tat 1219
Ile Ile Asp Asn Asn Glu Arg Arg Lys Glu Leu Ser Lys Ser Ser Tyr
360 365 370
gct gcc gta cac agc tca gat cag ccc tac gcg ccc ttt gtg tgg ctc 1267
Ala Ala Val His Ser Ser Asp Gln Pro Tyr Ala Pro Phe Val Trp Leu
375 380 385
tct gag gca cca agc ggc att ctt ggt ctc att gcc tca cag ctc act 1315
Ser Glu Ala Pro Ser Gly Ile Leu Gly Leu Ile Ala Ser Gln Leu Thr
390 395 400 405
cgt gag tct gac gtg cct gcc att gtc att aat cca gat acc ttg tcc 1363
Arg Glu Ser Asp Val Pro Ala Ile Val Ile Asn Pro Asp Thr Leu Ser
410 415 420
ggt tca gct cgc tca cct gag tgg gca ccg atc atc acc caa gta aac 1411
Gly Ser Ala Arg Ser Pro Glu Trp Ala Pro Ile Ile Thr Gln Val Asn
425 430 435
acc ctc agc gca caa ggt cac ggc ggt att cat gct gca ggc cat gag 1459
Thr Leu Ser Ala Gln Gly His Gly Gly Ile His Ala Ala Gly His Glu
440 445 450
tac gcc tgt ggt atg cgt ttt gat aac cat gat gac att gtg acc ttt 1507
Tyr Ala Cys Gly Met Arg Phe Asp Asn His Asp Asp Ile Val Thr Phe
455 460 465
gtt gca aca ctc gac gca ctc gat aaa aac acg cca cgg gaa gca cag 1555
Val Ala Thr Leu Asp Ala Leu Asp Lys Asn Thr Pro Arg Glu Ala Gln
470 475 480 485
ccg gca gat ctg cat ttg gtt gac att gac cac gcg cgt cct gtg ctt 1603
Pro Ala Asp Leu His Leu Val Asp Ile Asp His Ala Arg Pro Val Leu
490 495 500
gat aac ccc tca ctc acc caa gag ctc agt acg gtc gat gct gca gtg 1651
Asp Asn Pro Ser Leu Thr Gln Glu Leu Ser Thr Val Asp Ala Ala Val
505 510 515
gat gct gca cag ttg ctt gtt ctc att gat cag ctt gat caa ctg cag 1699
Asp Ala Ala Gln Leu Leu Val Leu Ile Asp Gln Leu Asp Gln Leu Gln
520 525 530
cca ttt gga cat ggt ttt acc tat ccg cgc atc gac gtg acg ttc agg 1747
Pro Phe Gly His Gly Phe Thr Tyr Pro Arg Ile Asp Val Thr Phe Arg
535 540 545
ccg gca gaa aca gaa ttc aag gtt atg ggt cag cac cat caa cat ctc 1795
Pro Ala Glu Thr Glu Phe Lys Val Met Gly Gln His His Gln His Leu
550 555 560 565
aag gtg atc act cac tca ggg ttg acc tta ttg tgg tgg aat aag gct 1843
Lys Val Ile Thr His Ser Gly Leu Thr Leu Leu Trp Trp Asn Lys Ala
570 575 580
cag cag ctc gat gag atc gca cag tct gaa tta gtc acc atg tct gtg 1891
Gln Gln Leu Asp Glu Ile Ala Gln Ser Glu Leu Val Thr Met Ser Val
585 590 595
gag ctc gat gtc aat atg ttc cgt ggg ttt att tcc ccg caa ggc att 1939
Glu Leu Asp Val Asn Met Phe Arg Gly Phe Ile Ser Pro Gln Gly Ile
600 605 610
gtc tct gcg tgc aca gtt atc tagcttggtt gcataagcac caaaaacaac 1990
Val Ser Ala Cys Thr Val Ile
615 620
<210> 2
<211> 620
<212> PRT
<213> Corynebacterium glutamicum
<400> 2
Met Thr Val Thr Ser Pro Ala Ala Leu Ala Leu Ser Asp Met Ser Tyr
1 5 10 15
Val Asp Ile Ile Lys Lys Lys Arg Gly Trp Thr Thr Glu Phe Phe His
20 25 30
Ser Thr Ile Asn Thr Gly Glu Thr Thr Thr Pro Leu Pro Asp Ser Asp
35 40 45
Arg Ala Thr Ala Leu Ile His Asp His Ile Thr Lys Ala Gln Glu Ile
50 55 60
Thr Ile Ile Thr Asp Phe Asp Met Asp Gly Ile Ser Ala Gly Val Ile
65 70 75 80
Ala Tyr Ala Gly Leu Ala Glu Leu Gly Ala Gln Val Asn Met Val Val
85 90 95
Pro Asp Tyr Arg Gly Glu Arg Asn Val Thr Ala Ser Asp Ile Asp Arg
100 105 110
Ala Leu Glu Leu Tyr Pro Ala Thr Ser Leu Ile Ile Thr Cys Asp Val
115 120 125
Gly Ile Gly Ser His Glu Gly Ile Ala Arg Ala His Glu Arg Ser Ile
130 135 140
Ala Val Leu Val Thr Asp His His Met Glu Val Glu Pro Cys Gln Ala
145 150 155 160
Asp Val Val Leu Asn Pro Asn Arg Ile Asp Ser Asp Tyr Pro Asn Lys
165 170 175
Asp Ile Cys Gly Ala Gln Val Ile Phe Ala Thr Leu Ser Asp Tyr Ala
180 185 190
Arg Arg Tyr Arg Ala Asp Lys Ile Ile Asp Ile Asn Leu Leu Ala Val
195 200 205
Phe Ser Gly Ile Gly Ala Leu Ala Asp Val Met Pro Leu Thr Arg Asp
210 215 220
Thr Arg Pro Thr Val Lys Gln Ala Ile Ala Leu Leu Arg Leu Ala Ile
225 230 235 240
Pro Gln Val Ser Lys Asn Arg Phe Gly Gly Trp Asp Thr Tyr Ala Ala
245 250 255
Arg Ser Val Asn Pro Asp Thr Ser Thr Leu Met His Ile Val Asn Ala
260 265 270
Ser Gln His Asp His Arg Phe Ile Ala Ala Phe Gln Gly Ile Ser Ile
275 280 285
Leu Leu Gly Glu Leu Ile Ala Gln Lys Lys Leu Val Asn Ile Asp Asn
290 295 300
Ile Ser Glu Ser Phe Ile Gly Phe Thr Leu Gly Pro Met Phe Asn Ala
305 310 315 320
Thr Arg Arg Val Gly Gly Asp Met His Asp Ser Phe Leu Val Phe Ala
325 330 335
Pro His Ala Ala Leu Ala Ser Gln Pro Ser Met Asn Pro Asn Arg His
340 345 350
Ala Ala Ile Ser Arg Ile Ile Asp Asn Asn Glu Arg Arg Lys Glu Leu
355 360 365
Ser Lys Ser Ser Tyr Ala Ala Val His Ser Ser Asp Gln Pro Tyr Ala
370 375 380
Pro Phe Val Trp Leu Ser Glu Ala Pro Ser Gly Ile Leu Gly Leu Ile
385 390 395 400
Ala Ser Gln Leu Thr Arg Glu Ser Asp Val Pro Ala Ile Val Ile Asn
405 410 415
Pro Asp Thr Leu Ser Gly Ser Ala Arg Ser Pro Glu Trp Ala Pro Ile
420 425 430
Ile Thr Gln Val Asn Thr Leu Ser Ala Gln Gly His Gly Gly Ile His
435 440 445
Ala Ala Gly His Glu Tyr Ala Cys Gly Met Arg Phe Asp Asn His Asp
450 455 460
Asp Ile Val Thr Phe Val Ala Thr Leu Asp Ala Leu Asp Lys Asn Thr
465 470 475 480
Pro Arg Glu Ala Gln Pro Ala Asp Leu His Leu Val Asp Ile Asp His
485 490 495
Ala Arg Pro Val Leu Asp Asn Pro Ser Leu Thr Gln Glu Leu Ser Thr
500 505 510
Val Asp Ala Ala Val Asp Ala Ala Gln Leu Leu Val Leu Ile Asp Gln
515 520 525
Leu Asp Gln Leu Gln Pro Phe Gly His Gly Phe Thr Tyr Pro Arg Ile
530 535 540
Asp Val Thr Phe Arg Pro Ala Glu Thr Glu Phe Lys Val Met Gly Gln
545 550 555 560
His His Gln His Leu Lys Val Ile Thr His Ser Gly Leu Thr Leu Leu
565 570 575
Trp Trp Asn Lys Ala Gln Gln Leu Asp Glu Ile Ala Gln Ser Glu Leu
580 585 590
Val Thr Met Ser Val Glu Leu Asp Val Asn Met Phe Arg Gly Phe Ile
595 600 605
Ser Pro Gln Gly Ile Val Ser Ala Cys Thr Val Ile
610 615 620
<210> 3
<211> 2845
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2815)
<223> RXA00061
<400> 3
aatcaattgc agaactaacc cggttgtttc cgagccagtc tgaatgactg aaagcaatat 60
tagaccatca atgattagga atggaaatta ggggtctggt ttg ggt gaa tgt gtc 115
Leu Gly Glu Cys Val
1 5
gct aat ttt tcc act cgc cta cac tcg gga ggc gtg act gag aag act 163
Ala Asn Phe Ser Thr Arg Leu His Ser Gly Gly Val Thr Glu Lys Thr
10 15 20
gac cag acc tta atg ctt atc gac ggc cac tcg atg gct ttc cgc gca 211
Asp Gln Thr Leu Met Leu Ile Asp Gly His Ser Met Ala Phe Arg Ala
25 30 35
ttc ttt gct ttg ccg gct gag aat ttc tcc acg tcg ggc ggg cag gcc 259
Phe Phe Ala Leu Pro Ala Glu Asn Phe Ser Thr Ser Gly Gly Gln Ala
40 45 50
acc aat gct gtc tat ggc ttt ctc tcg atg ctg tcc acg ttg ttg aag 307
Thr Asn Ala Val Tyr Gly Phe Leu Ser Met Leu Ser Thr Leu Leu Lys
55 60 65
gat gag cag cct act cat gtg gcg gtg gct ttc gat gtg ggg cgt aag 355
Asp Glu Gln Pro Thr His Val Ala Val Ala Phe Asp Val Gly Arg Lys
70 75 80 85
acg ttc cgt acc gat atg ttc ccg gcg tat aag gcg cag cgt gaa gca 403
Thr Phe Arg Thr Asp Met Phe Pro Ala Tyr Lys Ala Gln Arg Glu Ala
90 95 100
acg cca cct gag ttt aag ggc cag gtg gaa atc ctc aag gag gtg ttg 451
Thr Pro Pro Glu Phe Lys Gly Gln Val Glu Ile Leu Lys Glu Val Leu
105 110 115
tcc act ttg gga att acg act att gag aaa atc gat ttt gag gct gat 499
Ser Thr Leu Gly Ile Thr Thr Ile Glu Lys Ile Asp Phe Glu Ala Asp
120 125 130
gat gtg atc gcc acg ttg tct gtg gcg gcg aaa cct tta ggc ttt aag 547
Asp Val Ile Ala Thr Leu Ser Val Ala Ala Lys Pro Leu Gly Phe Lys
135 140 145
acg ctg att gtt acg ggt gac cgt gat tcc ttc cag ttg gtc aat gac 595
Thr Leu Ile Val Thr Gly Asp Arg Asp Ser Phe Gln Leu Val Asn Asp
150 155 160 165
acc acc acg gtg ttg tat ccg atg aag ggc gtg tct gtg ctg cac cgt 643
Thr Thr Thr Val Leu Tyr Pro Met Lys Gly Val Ser Val Leu His Arg
170 175 180
ttc acg ccg gaa gca gtg gag gag aag tat gga ctg aca ccg agg cag 691
Phe Thr Pro Glu Ala Val Glu Glu Lys Tyr Gly Leu Thr Pro Arg Gln
185 190 195
tat ccg gag ttt gca gcg ctg cgt ggt gat cct tcc gat aac ttg cct 739
Tyr Pro Glu Phe Ala Ala Leu Arg Gly Asp Pro Ser Asp Asn Leu Pro
200 205 210
aat att cct ggc gtg ggc gag aag act gct acc aag tgg att gcc cag 787
Asn Ile Pro Gly Val Gly Glu Lys Thr Ala Thr Lys Trp Ile Ala Gln
215 220 225
tat gaa act ttg gat aat ttg ctt gat cac gct gat gag atc aag ggc 835
Tyr Glu Thr Leu Asp Asn Leu Leu Asp His Ala Asp Glu Ile Lys Gly
230 235 240 245
aag gtt ggc gcc agc ctg cgt gag cgc att gag cag gtc cgg atg aac 883
Lys Val Gly Ala Ser Leu Arg Glu Arg Ile Glu Gln Val Arg Met Asn
250 255 260
cgc aag ctc acg gag atg gtg aag gat ctg gag ctg ccg ctt ggt ccg 931
Arg Lys Leu Thr Glu Met Val Lys Asp Leu Glu Leu Pro Leu Gly Pro
265 270 275
gac gat ttt gag atg aag cct gtg cag gtt gcg gag gtt gcg gcg aag 979
Asp Asp Phe Glu Met Lys Pro Val Gln Val Ala Glu Val Ala Ala Lys
280 285 290
ttt gac gat ctg gag ttt ggt acc aat ttg cgt gag cgg gtg ctg gcg 1027
Phe Asp Asp Leu Glu Phe Gly Thr Asn Leu Arg Glu Arg Val Leu Ala
295 300 305
gtg gtg aag gcc gag ggt tcc gct gcc ccc gtg gag gaa gtg gaa gcg 1075
Val Val Lys Ala Glu Gly Ser Ala Ala Pro Val Glu Glu Val Glu Ala
310 315 320 325
gaa cag gtt gtc gtc gat acg caa tct ttg gcg caa tgg ctg cct gct 1123
Glu Gln Val Val Val Asp Thr Gln Ser Leu Ala Gln Trp Leu Pro Ala
330 335 340
agg gct ggc cag gcg ctt gct tta gcg ctg gct gga gtg gct aaa cct 1171
Arg Ala Gly Gln Ala Leu Ala Leu Ala Leu Ala Gly Val Ala Lys Pro
345 350 355
gct gct ggc gac acg tat gcg cta gcg att gcg gat acc aag cgc cat 1219
Ala Ala Gly Asp Thr Tyr Ala Leu Ala Ile Ala Asp Thr Lys Arg His
360 365 370
gcg gtg ttg gtt gat gtg gct gat att tca gcg gag gat gaa aag gcg 1267
Ala Val Leu Val Asp Val Ala Asp Ile Ser Ala Glu Asp Glu Lys Ala
375 380 385
ctg gcc acg tgg ttg gcg tcg gaa gat cca aag atg ctg cac ggc gct 1315
Leu Ala Thr Trp Leu Ala Ser Glu Asp Pro Lys Met Leu His Gly Ala
390 395 400 405
aag gcc gcc tat cat atg ctc gct ggg cgc ggt ttt gag ctg cac ggc 1363
Lys Ala Ala Tyr His Met Leu Ala Gly Arg Gly Phe Glu Leu His Gly
410 415 420
gtg gtg cat gac acg gcg atc gcg gca tac ttg ctg cgt ccg ggc caa 1411
Val Val His Asp Thr Ala Ile Ala Ala Tyr Leu Leu Arg Pro Gly Gln
425 430 435
cgc acc tat gag ctt gcc gac gtc tac cag cgg cat ctt caa cga cag 1459
Arg Thr Tyr Glu Leu Ala Asp Val Tyr Gln Arg His Leu Gln Arg Gln
440 445 450
ttg tct aca aac gac aat ggc ggc cag ctc acg ctg ctc gac gca gct 1507
Leu Ser Thr Asn Asp Asn Gly Gly Gln Leu Thr Leu Leu Asp Ala Ala
455 460 465
gat gac caa tcg ctt gtt gat gat gtc att gca atc ctt gag ctg tct 1555
Asp Asp Gln Ser Leu Val Asp Asp Val Ile Ala Ile Leu Glu Leu Ser
470 475 480 485
gaa gaa ttg acc aaa cag ctt cag gag att caa gct ttt gag ctt tac 1603
Glu Glu Leu Thr Lys Gln Leu Gln Glu Ile Gln Ala Phe Glu Leu Tyr
490 495 500
cat gac ctg gaa att ccg ctg tcg gga att ctg gcg cgc atg gag gcc 1651
His Asp Leu Glu Ile Pro Leu Ser Gly Ile Leu Ala Arg Met Glu Ala
505 510 515
atc ggt atc gct gtt gat gtt gcc act ttg gaa gag cag ttg aag act 1699
Ile Gly Ile Ala Val Asp Val Ala Thr Leu Glu Glu Gln Leu Lys Thr
520 525 530
ttc att ggt cag gtt gct cag gaa gag gaa gca gct cgc gag ctc gct 1747
Phe Ile Gly Gln Val Ala Gln Glu Glu Glu Ala Ala Arg Glu Leu Ala
535 540 545
gag gat cca acc ctg aat ctc tcg agc ccg aag cag ctg caa gtg gtg 1795
Glu Asp Pro Thr Leu Asn Leu Ser Ser Pro Lys Gln Leu Gln Val Val
550 555 560 565
ctt ttt gag acg ttc gga atg ccg aaa acc aag aaa acc aag acc ggc 1843
Leu Phe Glu Thr Phe Gly Met Pro Lys Thr Lys Lys Thr Lys Thr Gly
570 575 580
tac tct acg gct gcc gcg gaa att gaa gcc cta gcg atc aag aat ccg 1891
Tyr Ser Thr Ala Ala Ala Glu Ile Glu Ala Leu Ala Ile Lys Asn Pro
585 590 595
cac cca ttc cta gat cac ctg ttg gca cac cgt cag tac caa aag atg 1939
His Pro Phe Leu Asp His Leu Leu Ala His Arg Gln Tyr Gln Lys Met
600 605 610
aag acc act ctg gaa ggt ctc atc cgt gag gtg gct cct gat ggc cgt 1987
Lys Thr Thr Leu Glu Gly Leu Ile Arg Glu Val Ala Pro Asp Gly Arg
615 620 625
att cac acc acc ttc aac cag acg gtg gcg tct acg gga cgt ttg tca 2035
Ile His Thr Thr Phe Asn Gln Thr Val Ala Ser Thr Gly Arg Leu Ser
630 635 640 645
tcc act gat ccc aac ctg caa aac att cct gtg cgc act gag gct ggc 2083
Ser Thr Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Glu Ala Gly
650 655 660
cga aag att cgt tcg gga ttc gtc gta ggc gag ggg tat gaa acc ttg 2131
Arg Lys Ile Arg Ser Gly Phe Val Val Gly Glu Gly Tyr Glu Thr Leu
665 670 675
ctg act gcc gac tat tcg cag att gaa atg cgc gtg atg gct cac ctt 2179
Leu Thr Ala Asp Tyr Ser Gln Ile Glu Met Arg Val Met Ala His Leu
680 685 690
tcc cag gac cca ggc ttg att gag gcg tac cgc gaa ggc gaa gac ctg 2227
Ser Gln Asp Pro Gly Leu Ile Glu Ala Tyr Arg Glu Gly Glu Asp Leu
695 700 705
cac aat tac gtg ggt tcc aag gtg ttt aat gtg ccc atc gat ggc gtg 2275
His Asn Tyr Val Gly Ser Lys Val Phe Asn Val Pro Ile Asp Gly Val
710 715 720 725
acc cct gag ctg cgt cgc cag gtc aag gcc atg tct tac ggt ctg gtg 2323
Thr Pro Glu Leu Arg Arg Gln Val Lys Ala Met Ser Tyr Gly Leu Val
730 735 740
tac ggc ttg tcc gcg ttt ggt ttg tct cag cag ctg agc att cct gct 2371
Tyr Gly Leu Ser Ala Phe Gly Leu Ser Gln Gln Leu Ser Ile Pro Ala
745 750 755
ggc gaa gcg aag cag atc atg gag tcc tac ttc gag cgc ttc ggc gga 2419
Gly Glu Ala Lys Gln Ile Met Glu Ser Tyr Phe Glu Arg Phe Gly Gly
760 765 770
gta cag cgc tac ctc cgg gag atc gtg gag gag gct cga aaa gct ggc 2467
Val Gln Arg Tyr Leu Arg Glu Ile Val Glu Glu Ala Arg Lys Ala Gly
775 780 785
tac acg gaa acg ctg ttt ggg cgt cgt cgc tac ctg ccg gaa ctg acc 2515
Tyr Thr Glu Thr Leu Phe Gly Arg Arg Arg Tyr Leu Pro Glu Leu Thr
790 795 800 805
tcg gat aac cgt gtc gct cgt gaa aac gct gaa cgt gcc gca ctg aac 2563
Ser Asp Asn Arg Val Ala Arg Glu Asn Ala Glu Arg Ala Ala Leu Asn
810 815 820
gcc ccg att cag gga act gcc gca gac atc atc aag gtg gcc atg atc 2611
Ala Pro Ile Gln Gly Thr Ala Ala Asp Ile Ile Lys Val Ala Met Ile
825 830 835
cgg gtg gac cgt tca ctc aag gaa gct gcc gtg aaa tct cgc gtg ctg 2659
Arg Val Asp Arg Ser Leu Lys Glu Ala Ala Val Lys Ser Arg Val Leu
840 845 850
ctt cag gtg cat gat gaa ttg gtc gtg gaa gta gcg gcc ggt gag ttg 2707
Leu Gln Val His Asp Glu Leu Val Val Glu Val Ala Ala Gly Glu Leu
855 860 865
gaa caa gtc cgt gag att ctg gaa cgc gaa atg gat aac gcc atc aag 2755
Glu Gln Val Arg Glu Ile Leu Glu Arg Glu Met Asp Asn Ala Ile Lys
870 875 880 885
ctg tcc gtt cct ttg gaa gtt tca gct ggt gat ggc gtt aac tgg gat 2803
Leu Ser Val Pro Leu Glu Val Ser Ala Gly Asp Gly Val Asn Trp Asp
890 895 900
gct gca gcg cac taagaggtaa ctgccttttc gtcgacgagc 2845
Ala Ala Ala His
905
<210> 4
<211> 905
<212> PRT
<213> Corynebacterium glutamicum
<400> 4
Leu Gly Glu Cys Val Ala Asn Phe Ser Thr Arg Leu His Ser Gly Gly
1 5 10 15
Val Thr Glu Lys Thr Asp Gln Thr Leu Met Leu Ile Asp Gly His Ser
20 25 30
Met Ala Phe Arg Ala Phe Phe Ala Leu Pro Ala Glu Asn Phe Ser Thr
35 40 45
Ser Gly Gly Gln Ala Thr Asn Ala Val Tyr Gly Phe Leu Ser Met Leu
50 55 60
Ser Thr Leu Leu Lys Asp Glu Gln Pro Thr His Val Ala Val Ala Phe
65 70 75 80
Asp Val Gly Arg Lys Thr Phe Arg Thr Asp Met Phe Pro Ala Tyr Lys
85 90 95
Ala Gln Arg Glu Ala Thr Pro Pro Glu Phe Lys Gly Gln Val Glu Ile
100 105 110
Leu Lys Glu Val Leu Ser Thr Leu Gly Ile Thr Thr Ile Glu Lys Ile
115 120 125
Asp Phe Glu Ala Asp Asp Val Ile Ala Thr Leu Ser Val Ala Ala Lys
130 135 140
Pro Leu Gly Phe Lys Thr Leu Ile Val Thr Gly Asp Arg Asp Ser Phe
145 150 155 160
Gln Leu Val Asn Asp Thr Thr Thr Val Leu Tyr Pro Met Lys Gly Val
165 170 175
Ser Val Leu His Arg Phe Thr Pro Glu Ala Val Glu Glu Lys Tyr Gly
180 185 190
Leu Thr Pro Arg Gln Tyr Pro Glu Phe Ala Ala Leu Arg Gly Asp Pro
195 200 205
Ser Asp Asn Leu Pro Asn Ile Pro Gly Val Gly Glu Lys Thr Ala Thr
210 215 220
Lys Trp Ile Ala Gln Tyr Glu Thr Leu Asp Asn Leu Leu Asp His Ala
225 230 235 240
Asp Glu Ile Lys Gly Lys Val Gly Ala Ser Leu Arg Glu Arg Ile Glu
245 250 255
Gln Val Arg Met Asn Arg Lys Leu Thr Glu Met Val Lys Asp Leu Glu
260 265 270
Leu Pro Leu Gly Pro Asp Asp Phe Glu Met Lys Pro Val Gln Val Ala
275 280 285
Glu Val Ala Ala Lys Phe Asp Asp Leu Glu Phe Gly Thr Asn Leu Arg
290 295 300
Glu Arg Val Leu Ala Val Val Lys Ala Glu Gly Ser Ala Ala Pro Val
305 310 315 320
Glu Glu Val Glu Ala Glu Gln Val Val Val Asp Thr Gln Ser Leu Ala
325 330 335
Gln Trp Leu Pro Ala Arg Ala Gly Gln Ala Leu Ala Leu Ala Leu Ala
340 345 350
Gly Val Ala Lys Pro Ala Ala Gly Asp Thr Tyr Ala Leu Ala Ile Ala
355 360 365
Asp Thr Lys Arg His Ala Val Leu Val Asp Val Ala Asp Ile Ser Ala
370 375 380
Glu Asp Glu Lys Ala Leu Ala Thr Trp Leu Ala Ser Glu Asp Pro Lys
385 390 395 400
Met Leu His Gly Ala Lys Ala Ala Tyr His Met Leu Ala Gly Arg Gly
405 410 415
Phe Glu Leu His Gly Val Val His Asp Thr Ala Ile Ala Ala Tyr Leu
420 425 430
Leu Arg Pro Gly Gln Arg Thr Tyr Glu Leu Ala Asp Val Tyr Gln Arg
435 440 445
His Leu Gln Arg Gln Leu Ser Thr Asn Asp Asn Gly Gly Gln Leu Thr
450 455 460
Leu Leu Asp Ala Ala Asp Asp Gln Ser Leu Val Asp Asp Val Ile Ala
465 470 475 480
Ile Leu Glu Leu Ser Glu Glu Leu Thr Lys Gln Leu Gln Glu Ile Gln
485 490 495
Ala Phe Glu Leu Tyr His Asp Leu Glu Ile Pro Leu Ser Gly Ile Leu
500 505 510
Ala Arg Met Glu Ala Ile Gly Ile Ala Val Asp Val Ala Thr Leu Glu
515 520 525
Glu Gln Leu Lys Thr Phe Ile Gly Gln Val Ala Gln Glu Glu Glu Ala
530 535 540
Ala Arg Glu Leu Ala Glu Asp Pro Thr Leu Asn Leu Ser Ser Pro Lys
545 550 555 560
Gln Leu Gln Val Val Leu Phe Glu Thr Phe Gly Met Pro Lys Thr Lys
565 570 575
Lys Thr Lys Thr Gly Tyr Ser Thr Ala Ala Ala Glu Ile Glu Ala Leu
580 585 590
Ala Ile Lys Asn Pro His Pro Phe Leu Asp His Leu Leu Ala His Arg
595 600 605
Gln Tyr Gln Lys Met Lys Thr Thr Leu Glu Gly Leu Ile Arg Glu Val
610 615 620
Ala Pro Asp Gly Arg Ile His Thr Thr Phe Asn Gln Thr Val Ala Ser
625 630 635 640
Thr Gly Arg Leu Ser Ser Thr Asp Pro Asn Leu Gln Asn Ile Pro Val
645 650 655
Arg Thr Glu Ala Gly Arg Lys Ile Arg Ser Gly Phe Val Val Gly Glu
660 665 670
Gly Tyr Glu Thr Leu Leu Thr Ala Asp Tyr Ser Gln Ile Glu Met Arg
675 680 685
Val Met Ala His Leu Ser Gln Asp Pro Gly Leu Ile Glu Ala Tyr Arg
690 695 700
Glu Gly Glu Asp Leu His Asn Tyr Val Gly Ser Lys Val Phe Asn Val
705 710 715 720
Pro Ile Asp Gly Val Thr Pro Glu Leu Arg Arg Gln Val Lys Ala Met
725 730 735
Ser Tyr Gly Leu Val Tyr Gly Leu Ser Ala Phe Gly Leu Ser Gln Gln
740 745 750
Leu Ser Ile Pro Ala Gly Glu Ala Lys Gln Ile Met Glu Ser Tyr Phe
755 760 765
Glu Arg Phe Gly Gly Val Gln Arg Tyr Leu Arg Glu Ile Val Glu Glu
770 775 780
Ala Arg Lys Ala Gly Tyr Thr Glu Thr Leu Phe Gly Arg Arg Arg Tyr
785 790 795 800
Leu Pro Glu Leu Thr Ser Asp Asn Arg Val Ala Arg Glu Asn Ala Glu
805 810 815
Arg Ala Ala Leu Asn Ala Pro Ile Gln Gly Thr Ala Ala Asp Ile Ile
820 825 830
Lys Val Ala Met Ile Arg Val Asp Arg Ser Leu Lys Glu Ala Ala Val
835 840 845
Lys Ser Arg Val Leu Leu Gln Val His Asp Glu Leu Val Val Glu Val
850 855 860
Ala Ala Gly Glu Leu Glu Gln Val Arg Glu Ile Leu Glu Arg Glu Met
865 870 875 880
Asp Asn Ala Ile Lys Leu Ser Val Pro Leu Glu Val Ser Ala Gly Asp
885 890 895
Gly Val Asn Trp Asp Ala Ala Ala His
900 905
<210> 5
<211> 1621
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1591)
<223> RXA00209
<400> 5
acaagaccct cgatgctgcg gctgcgttgg accaagcgcc cgctgtcgag gatggacgtt 60
ttatggttcc gcagattctg ggtgagggcg actaataatt atg acc aac aag tac 115
Met Thr Asn Lys Tyr
1 5
ctg gtt gaa ggc tct gaa aac gag ctg acc aca aag acc gca gca gag 163
Leu Val Glu Gly Ser Glu Asn Glu Leu Thr Thr Lys Thr Ala Ala Glu
10 15 20
ctg gca ggt ctt att cat tcc cgc gag gta act tcc cgc gag gtt act 211
Leu Ala Gly Leu Ile His Ser Arg Glu Val Thr Ser Arg Glu Val Thr
25 30 35
caa gcg cac cta gat cgc att gct gcg gtt gac ggc gat att cat gca 259
Gln Ala His Leu Asp Arg Ile Ala Ala Val Asp Gly Asp Ile His Ala
40 45 50
ttt ctc cac gtt ggc cag gag gag gcc ctg aac gcg gcg gat gac gtc 307
Phe Leu His Val Gly Gln Glu Glu Ala Leu Asn Ala Ala Asp Asp Val
55 60 65
gat aag cgt cta gac gct gga gag gca cct gcc tcg gct ttg gct ggc 355
Asp Lys Arg Leu Asp Ala Gly Glu Ala Pro Ala Ser Ala Leu Ala Gly
70 75 80 85
gtg ccg ctt gcg ctg aag gat gtc ttt acc acc act gat gcg ccg acc 403
Val Pro Leu Ala Leu Lys Asp Val Phe Thr Thr Thr Asp Ala Pro Thr
90 95 100
acg gcg gca tcg aag atg ctt gag ggc tac atg agc cct tat gac gcg 451
Thr Ala Ala Ser Lys Met Leu Glu Gly Tyr Met Ser Pro Tyr Asp Ala
105 110 115
act gtg acc cgc aag atc cgt gag gct ggc atc cca att ttg ggt aag 499
Thr Val Thr Arg Lys Ile Arg Glu Ala Gly Ile Pro Ile Leu Gly Lys
120 125 130
acc aac atg gat gag ttt gcg atg ggt tcc tcc act gag aac tcc gca 547
Thr Asn Met Asp Glu Phe Ala Met Gly Ser Ser Thr Glu Asn Ser Ala
135 140 145
tac ggc cca acc cac aat ccg tgg gat ctg gag cgc acc gca ggt ggt 595
Tyr Gly Pro Thr His Asn Pro Trp Asp Leu Glu Arg Thr Ala Gly Gly
150 155 160 165
tct ggt ggt ggc tct tca gct gct ctt gct gca ggt cag gcg cca ctt 643
Ser Gly Gly Gly Ser Ser Ala Ala Leu Ala Ala Gly Gln Ala Pro Leu
170 175 180
gcg att ggt act gac act ggt gga tcc atc cgt cag cca gct gcg ctg 691
Ala Ile Gly Thr Asp Thr Gly Gly Ser Ile Arg Gln Pro Ala Ala Leu
185 190 195
acc aac act gtc ggt gtg aag cca acc tac gga acc gta tcc cgt tac 739
Thr Asn Thr Val Gly Val Lys Pro Thr Tyr Gly Thr Val Ser Arg Tyr
200 205 210
ggt ctg att gcg tgt gcg tcc tcc ctg gat cag ggt ggc cca acc gct 787
Gly Leu Ile Ala Cys Ala Ser Ser Leu Asp Gln Gly Gly Pro Thr Ala
215 220 225
cgt act gtt ctg gat acc gcg ctt ttg cac gag gtt atc gca ggc cac 835
Arg Thr Val Leu Asp Thr Ala Leu Leu His Glu Val Ile Ala Gly His
230 235 240 245
gac gct ttt gat gcg acc tcc gtg aat cgt ccg gtt gct cct gtt gtg 883
Asp Ala Phe Asp Ala Thr Ser Val Asn Arg Pro Val Ala Pro Val Val
250 255 260
cag gct gcc cgt gaa ggc gcg aac ggt gac ctg aaa ggc gtg aag gtc 931
Gln Ala Ala Arg Glu Gly Ala Asn Gly Asp Leu Lys Gly Val Lys Val
265 270 275
ggt gtg gtc aag cag ttc gac cgc gac ggc tac cag cct ggc gtg ctt 979
Gly Val Val Lys Gln Phe Asp Arg Asp Gly Tyr Gln Pro Gly Val Leu
280 285 290
gag gca ttc cac gct tct gtt gag cag atg cgc tcc cag ggt gcg gaa 1027
Glu Ala Phe His Ala Ser Val Glu Gln Met Arg Ser Gln Gly Ala Glu
295 300 305
atc gtc gag gtt gat tgc cct cac ttt gat gac gct ctt ggc gcg tac 1075
Ile Val Glu Val Asp Cys Pro His Phe Asp Asp Ala Leu Gly Ala Tyr
310 315 320 325
tac ctg att ctt cct tgt gaa gtt tcc tcc aac ctc gcg cgt ttt gac 1123
Tyr Leu Ile Leu Pro Cys Glu Val Ser Ser Asn Leu Ala Arg Phe Asp
330 335 340
ggc atg cgt tac ggt ttg cgc gct ggt gat gac gga act cgt tcc gcc 1171
Gly Met Arg Tyr Gly Leu Arg Ala Gly Asp Asp Gly Thr Arg Ser Ala
345 350 355
gat gag gtc atg gcg tac acc cgt gcg cag gga ttc ggc cct gag gtt 1219
Asp Glu Val Met Ala Tyr Thr Arg Ala Gln Gly Phe Gly Pro Glu Val
360 365 370
aag cgc cgt atc atc ctc ggc act tac gcg ttg tct gtt ggt tac tac 1267
Lys Arg Arg Ile Ile Leu Gly Thr Tyr Ala Leu Ser Val Gly Tyr Tyr
375 380 385
gac gcg tac tac ctg cag gct cag cgc gtt cgt acc ctc att gca cag 1315
Asp Ala Tyr Tyr Leu Gln Ala Gln Arg Val Arg Thr Leu Ile Ala Gln
390 395 400 405
gac ttc gcc aag gct tac gag cag gtc gac atc ttg gtg tcc cca acc 1363
Asp Phe Ala Lys Ala Tyr Glu Gln Val Asp Ile Leu Val Ser Pro Thr
410 415 420
act cca acc acc gcg ttc aag ctg ggg gag aag gtc acc gat ccg ctg 1411
Thr Pro Thr Thr Ala Phe Lys Leu Gly Glu Lys Val Thr Asp Pro Leu
425 430 435
gag atg tac aac ttc gac ttg tgc acc ctg cca ctg aac ctg gct ggt 1459
Glu Met Tyr Asn Phe Asp Leu Cys Thr Leu Pro Leu Asn Leu Ala Gly
440 445 450
ctc gcg ggc atg tcc ctg cct tcc ggc ttg gca tca gat act ggt ctg 1507
Leu Ala Gly Met Ser Leu Pro Ser Gly Leu Ala Ser Asp Thr Gly Leu
455 460 465
cct gtt ggt ttg cag ctg atg gct cct gct ttc cag gac gat cgt ctc 1555
Pro Val Gly Leu Gln Leu Met Ala Pro Ala Phe Gln Asp Asp Arg Leu
470 475 480 485
tac cgc gtc ggc gct gct ttt gaa gct gga cgc aag taggttctaa 1601
Tyr Arg Val Gly Ala Ala Phe Glu Ala Gly Arg Lys
490 495
acccttttta agaaattggc 1621
<210> 6
<211> 497
<212> PRT
<213> Corynebacterium glutamicum
<400> 6
Met Thr Asn Lys Tyr Leu Val Glu Gly Ser Glu Asn Glu Leu Thr Thr
1 5 10 15
Lys Thr Ala Ala Glu Leu Ala Gly Leu Ile His Ser Arg Glu Val Thr
20 25 30
Ser Arg Glu Val Thr Gln Ala His Leu Asp Arg Ile Ala Ala Val Asp
35 40 45
Gly Asp Ile His Ala Phe Leu His Val Gly Gln Glu Glu Ala Leu Asn
50 55 60
Ala Ala Asp Asp Val Asp Lys Arg Leu Asp Ala Gly Glu Ala Pro Ala
65 70 75 80
Ser Ala Leu Ala Gly Val Pro Leu Ala Leu Lys Asp Val Phe Thr Thr
85 90 95
Thr Asp Ala Pro Thr Thr Ala Ala Ser Lys Met Leu Glu Gly Tyr Met
100 105 110
Ser Pro Tyr Asp Ala Thr Val Thr Arg Lys Ile Arg Glu Ala Gly Ile
115 120 125
Pro Ile Leu Gly Lys Thr Asn Met Asp Glu Phe Ala Met Gly Ser Ser
130 135 140
Thr Glu Asn Ser Ala Tyr Gly Pro Thr His Asn Pro Trp Asp Leu Glu
145 150 155 160
Arg Thr Ala Gly Gly Ser Gly Gly Gly Ser Ser Ala Ala Leu Ala Ala
165 170 175
Gly Gln Ala Pro Leu Ala Ile Gly Thr Asp Thr Gly Gly Ser Ile Arg
180 185 190
Gln Pro Ala Ala Leu Thr Asn Thr Val Gly Val Lys Pro Thr Tyr Gly
195 200 205
Thr Val Ser Arg Tyr Gly Leu Ile Ala Cys Ala Ser Ser Leu Asp Gln
210 215 220
Gly Gly Pro Thr Ala Arg Thr Val Leu Asp Thr Ala Leu Leu His Glu
225 230 235 240
Val Ile Ala Gly His Asp Ala Phe Asp Ala Thr Ser Val Asn Arg Pro
245 250 255
Val Ala Pro Val Val Gln Ala Ala Arg Glu Gly Ala Asn Gly Asp Leu
260 265 270
Lys Gly Val Lys Val Gly Val Val Lys Gln Phe Asp Arg Asp Gly Tyr
275 280 285
Gln Pro Gly Val Leu Glu Ala Phe His Ala Ser Val Glu Gln Met Arg
290 295 300
Ser Gln Gly Ala Glu Ile Val Glu Val Asp Cys Pro His Phe Asp Asp
305 310 315 320
Ala Leu Gly Ala Tyr Tyr Leu Ile Leu Pro Cys Glu Val Ser Ser Asn
325 330 335
Leu Ala Arg Phe Asp Gly Met Arg Tyr Gly Leu Arg Ala Gly Asp Asp
340 345 350
Gly Thr Arg Ser Ala Asp Glu Val Met Ala Tyr Thr Arg Ala Gln Gly
355 360 365
Phe Gly Pro Glu Val Lys Arg Arg Ile Ile Leu Gly Thr Tyr Ala Leu
370 375 380
Ser Val Gly Tyr Tyr Asp Ala Tyr Tyr Leu Gln Ala Gln Arg Val Arg
385 390 395 400
Thr Leu Ile Ala Gln Asp Phe Ala Lys Ala Tyr Glu Gln Val Asp Ile
405 410 415
Leu Val Ser Pro Thr Thr Pro Thr Thr Ala Phe Lys Leu Gly Glu Lys
420 425 430
Val Thr Asp Pro Leu Glu Met Tyr Asn Phe Asp Leu Cys Thr Leu Pro
435 440 445
Leu Asn Leu Ala Gly Leu Ala Gly Met Ser Leu Pro Ser Gly Leu Ala
450 455 460
Ser Asp Thr Gly Leu Pro Val Gly Leu Gln Leu Met Ala Pro Ala Phe
465 470 475 480
Gln Asp Asp Arg Leu Tyr Arg Val Gly Ala Ala Phe Glu Ala Gly Arg
485 490 495
Lys
<210> 7
<211> 793
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(763)
<223> RXA00211
<400> 7
tgagccaaaa tcaataaggt gtttttcagc ctgaggtaaa aatacggtgg tactgtcgaa 60
accaatcatc ccctagtttt gaaaagaagg aagcgagcca atg tca ttc ctg atc 115
Met Ser Phe Leu Ile
1 5
cgc gtc ctg ttg tcc gac acc cca ggc agc ctc gcg tta ctc gct gaa 163
Arg Val Leu Leu Ser Asp Thr Pro Gly Ser Leu Ala Leu Leu Ala Glu
10 15 20
gcc ctt ggg att gta gag gcc aat att caa tcc gtg gac gtg gtg gaa 211
Ala Leu Gly Ile Val Glu Ala Asn Ile Gln Ser Val Asp Val Val Glu
25 30 35
cgc ttc ccc aat ggc acg gtc atg gac gat ctg gtg atc tcc atc cct 259
Arg Phe Pro Asn Gly Thr Val Met Asp Asp Leu Val Ile Ser Ile Pro
40 45 50
cgc gat gtc atg gca gac acc atc atc acc gca gct gaa gaa gtc gac 307
Arg Asp Val Met Ala Asp Thr Ile Ile Thr Ala Ala Glu Glu Val Asp
55 60 65
ggc gtg gag att gat tcc atc cgc cca ttc tcc ggg act gtt gac cgc 355
Gly Val Glu Ile Asp Ser Ile Arg Pro Phe Ser Gly Thr Val Asp Arg
70 75 80 85
cgc gga cag atc caa atg ctg gct gct gtt gct cac caa cgc cgc gat 403
Arg Gly Gln Ile Gln Met Leu Ala Ala Val Ala His Gln Arg Arg Asp
90 95 100
atc acc gca gcg atg gaa gaa atg gtc gat gtc atc ccc cgc acc atg 451
Ile Thr Ala Ala Met Glu Glu Met Val Asp Val Ile Pro Arg Thr Met
105 110 115
acc tct ggt tgg gct ttg gtc att gat cta aaa gga ccc atc act cgc 499
Thr Ser Gly Trp Ala Leu Val Ile Asp Leu Lys Gly Pro Ile Thr Arg
120 125 130
atc gct ggt tcc cta gca gcg ccc gaa gat gac ggc acc gtt ccg gag 547
Ile Ala Gly Ser Leu Ala Ala Pro Glu Asp Asp Gly Thr Val Pro Glu
135 140 145
aac atc gtt ctc aaa gaa gct cgc atg ctc aac ccg gaa aac gat ccg 595
Asn Ile Val Leu Lys Glu Ala Arg Met Leu Asn Pro Glu Asn Asp Pro
150 155 160 165
tgg att cca gag tcc tgg aca ctg ctt gat tct tcc ctt gcc atc gct 643
Trp Ile Pro Glu Ser Trp Thr Leu Leu Asp Ser Ser Leu Ala Ile Ala
170 175 180
ccg atc ggc aag cac ggc ctg gct ctg att atc ggt cgc cct ggt ggc 691
Pro Ile Gly Lys His Gly Leu Ala Leu Ile Ile Gly Arg Pro Gly Gly
185 190 195
cct gat ttc ttg gcc agc gaa gtg gag cac tta ggc caa gtc ggt gac 739
Pro Asp Phe Leu Ala Ser Glu Val Glu His Leu Gly Gln Val Gly Asp
200 205 210
att atc gga gca atg ctt caa aaa taatctgagc tgtttaaaaa atgccccaag 793
Ile Ile Gly Ala Met Leu Gln Lys
215 220
<210> 8
<211> 221
<212> PRT
<213> Corynebacterium glutamicum
<400> 8
Met Ser Phe Leu Ile Arg Val Leu Leu Ser Asp Thr Pro Gly Ser Leu
1 5 10 15
Ala Leu Leu Ala Glu Ala Leu Gly Ile Val Glu Ala Asn Ile Gln Ser
20 25 30
Val Asp Val Val Glu Arg Phe Pro Asn Gly Thr Val Met Asp Asp Leu
35 40 45
Val Ile Ser Ile Pro Arg Asp Val Met Ala Asp Thr Ile Ile Thr Ala
50 55 60
Ala Glu Glu Val Asp Gly Val Glu Ile Asp Ser Ile Arg Pro Phe Ser
65 70 75 80
Gly Thr Val Asp Arg Arg Gly Gln Ile Gln Met Leu Ala Ala Val Ala
85 90 95
His Gln Arg Arg Asp Ile Thr Ala Ala Met Glu Glu Met Val Asp Val
100 105 110
Ile Pro Arg Thr Met Thr Ser Gly Trp Ala Leu Val Ile Asp Leu Lys
115 120 125
Gly Pro Ile Thr Arg Ile Ala Gly Ser Leu Ala Ala Pro Glu Asp Asp
130 135 140
Gly Thr Val Pro Glu Asn Ile Val Leu Lys Glu Ala Arg Met Leu Asn
145 150 155 160
Pro Glu Asn Asp Pro Trp Ile Pro Glu Ser Trp Thr Leu Leu Asp Ser
165 170 175
Ser Leu Ala Ile Ala Pro Ile Gly Lys His Gly Leu Ala Leu Ile Ile
180 185 190
Gly Arg Pro Gly Gly Pro Asp Phe Leu Ala Ser Glu Val Glu His Leu
195 200 205
Gly Gln Val Gly Asp Ile Ile Gly Ala Met Leu Gln Lys
210 215 220
<210> 9
<211> 1543
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1513)
<223> RXA00314
<400> 9
acctgtaaac acttacggtt tgggcgaaat tgaagcggga gccaacctgc tcaacgtcgc 60
aaagaaagaa gcggtgccag caacaccata agttgaaacc ttg agt gtt cgc aca 115
Leu Ser Val Arg Thr
1 5
cag gtt aga cta ggg gac gtg act cta cgc atc ttt gac acc ggt acc 163
Gln Val Arg Leu Gly Asp Val Thr Leu Arg Ile Phe Asp Thr Gly Thr
10 15 20
cgt acg ctt cga gat ttt aaa cct gtt caa cca ggt cat gcc tcg gtg 211
Arg Thr Leu Arg Asp Phe Lys Pro Val Gln Pro Gly His Ala Ser Val
25 30 35
tac ctg tgt ggt gcc acc ccg caa tct tca ccc cac att gga cat gtt 259
Tyr Leu Cys Gly Ala Thr Pro Gln Ser Ser Pro His Ile Gly His Val
40 45 50
cgt tca gca gta gcg ttt gat att ttg cgc cgc tgg ctc atg gct aag 307
Arg Ser Ala Val Ala Phe Asp Ile Leu Arg Arg Trp Leu Met Ala Lys
55 60 65
gga ctt gat gtg gca ttt gtt cgc aat gtc act gat atc gat gac aag 355
Gly Leu Asp Val Ala Phe Val Arg Asn Val Thr Asp Ile Asp Asp Lys
70 75 80 85
att ctc acc aag gca tct gaa aat ggt cgc cct tgg tgg gaa tgg gtg 403
Ile Leu Thr Lys Ala Ser Glu Asn Gly Arg Pro Trp Trp Glu Trp Val
90 95 100
tcc acc tat gaa cgt gaa ttc acc tgg acg tac aac acg ttg ggt gtg 451
Ser Thr Tyr Glu Arg Glu Phe Thr Trp Thr Tyr Asn Thr Leu Gly Val
105 110 115
ctt cct cca tca acg gag cct cgt gca aca ggc cac gtc act cag atg 499
Leu Pro Pro Ser Thr Glu Pro Arg Ala Thr Gly His Val Thr Gln Met
120 125 130
att aag tac atg cag cgc ttg att gat aac ggc ttt gct tac gcc gtt 547
Ile Lys Tyr Met Gln Arg Leu Ile Asp Asn Gly Phe Ala Tyr Ala Val
135 140 145
gat ggc tct gtg tac ttt gat gtc gca gcg tgg tcc aag gct gaa gga 595
Asp Gly Ser Val Tyr Phe Asp Val Ala Ala Trp Ser Lys Ala Glu Gly
150 155 160 165
tct gac tat ggt tct ttg tcc gga aac cgt gtt gaa gat atg gag cag 643
Ser Asp Tyr Gly Ser Leu Ser Gly Asn Arg Val Glu Asp Met Glu Gln
170 175 180
ggc gag ccc gat aac ttt ggt aag cgg ggg cca cag gac ttt gct ctg 691
Gly Glu Pro Asp Asn Phe Gly Lys Arg Gly Pro Gln Asp Phe Ala Leu
185 190 195
tgg aag gct gcc aaa ccg ggt gag ccg tca tgg cca acc cct tgg gga 739
Trp Lys Ala Ala Lys Pro Gly Glu Pro Ser Trp Pro Thr Pro Trp Gly
200 205 210
gac ggc cgg ccg ggt tgg cat ttg gaa tgc tct gcc atg gcc acc tac 787
Asp Gly Arg Pro Gly Trp His Leu Glu Cys Ser Ala Met Ala Thr Tyr
215 220 225
tat ttg ggt gag caa ttt gat att cac tgt ggt ggt ttg gat ctg caa 835
Tyr Leu Gly Glu Gln Phe Asp Ile His Cys Gly Gly Leu Asp Leu Gln
230 235 240 245
ttt cca cac cat gaa aat gaa att gcc cag gca cat gcg gct ggc gat 883
Phe Pro His His Glu Asn Glu Ile Ala Gln Ala His Ala Ala Gly Asp
250 255 260
aaa ttt gcc aac tac tgg atg cac aat cac tgg gta aca atg gcc ggc 931
Lys Phe Ala Asn Tyr Trp Met His Asn His Trp Val Thr Met Ala Gly
265 270 275
gag aaa atg tcc aag tct ttg ggc aat gtt ttg gct gtg ccg gaa atg 979
Glu Lys Met Ser Lys Ser Leu Gly Asn Val Leu Ala Val Pro Glu Met
280 285 290
cta aag cag gtt cgt cct gtc gag ctt cgt tat tac ctt ggg tct gcc 1027
Leu Lys Gln Val Arg Pro Val Glu Leu Arg Tyr Tyr Leu Gly Ser Ala
295 300 305
cat tac cgt tcc gtc ctt gag tat tcc gag agc gct ttg agt gaa gct 1075
His Tyr Arg Ser Val Leu Glu Tyr Ser Glu Ser Ala Leu Ser Glu Ala
310 315 320 325
gcg gtg ggt tac cgt cgc att gag tct ttc ctt gag cgt gtg ggg gat 1123
Ala Val Gly Tyr Arg Arg Ile Glu Ser Phe Leu Glu Arg Val Gly Asp
330 335 340
gtt gag gta ggc gag tgg acg cca ggt ttt gaa gtt gcg atg gat gag 1171
Val Glu Val Gly Glu Trp Thr Pro Gly Phe Glu Val Ala Met Asp Glu
345 350 355
gat att gca gtt cct aag gct ttg gct gaa atc cat aac gct gtc cgc 1219
Asp Ile Ala Val Pro Lys Ala Leu Ala Glu Ile His Asn Ala Val Arg
360 365 370
gag ggc aat gct gcc ttg gat aag ggt gat cgt gag gca gcg gag aag 1267
Glu Gly Asn Ala Ala Leu Asp Lys Gly Asp Arg Glu Ala Ala Glu Lys
375 380 385
ctt gct tcc tcg gtt cgt gcg atg act ggc gtt ttg ggc ttc gac ccc 1315
Leu Ala Ser Ser Val Arg Ala Met Thr Gly Val Leu Gly Phe Asp Pro
390 395 400 405
gtt gaa tgg ggt tca gat gca ggc gct gat ggc aag gca gat aag gcg 1363
Val Glu Trp Gly Ser Asp Ala Gly Ala Asp Gly Lys Ala Asp Lys Ala
410 415 420
ctt gat gtg ctg att tct tcg gag ctt gag cgt cgt gca act gct cgt 1411
Leu Asp Val Leu Ile Ser Ser Glu Leu Glu Arg Arg Ala Thr Ala Arg
425 430 435
gct gag aag aat tgg gcg gtt gct gat gag gtt cga gat cgt ctt gcc 1459
Ala Glu Lys Asn Trp Ala Val Ala Asp Glu Val Arg Asp Arg Leu Ala
440 445 450
gat gct ggt att gag gtt gtg gat acc gca gat ggc gct aca tgg aaa 1507
Asp Ala Gly Ile Glu Val Val Asp Thr Ala Asp Gly Ala Thr Trp Lys
455 460 465
ttg cag taattacaga cacttttaag gagataattt 1543
Leu Gln
470
<210> 10
<211> 471
<212> PRT
<213> Corynebacterium glutamicum
<400> 10
Leu Ser Val Arg Thr Gln Val Arg Leu Gly Asp Val Thr Leu Arg Ile
1 5 10 15
Phe Asp Thr Gly Thr Arg Thr Leu Arg Asp Phe Lys Pro Val Gln Pro
20 25 30
Gly His Ala Ser Val Tyr Leu Cys Gly Ala Thr Pro Gln Ser Ser Pro
35 40 45
His Ile Gly His Val Arg Ser Ala Val Ala Phe Asp Ile Leu Arg Arg
50 55 60
Trp Leu Met Ala Lys Gly Leu Asp Val Ala Phe Val Arg Asn Val Thr
65 70 75 80
Asp Ile Asp Asp Lys Ile Leu Thr Lys Ala Ser Glu Asn Gly Arg Pro
85 90 95
Trp Trp Glu Trp Val Ser Thr Tyr Glu Arg Glu Phe Thr Trp Thr Tyr
100 105 110
Asn Thr Leu Gly Val Leu Pro Pro Ser Thr Glu Pro Arg Ala Thr Gly
115 120 125
His Val Thr Gln Met Ile Lys Tyr Met Gln Arg Leu Ile Asp Asn Gly
130 135 140
Phe Ala Tyr Ala Val Asp Gly Ser Val Tyr Phe Asp Val Ala Ala Trp
145 150 155 160
Ser Lys Ala Glu Gly Ser Asp Tyr Gly Ser Leu Ser Gly Asn Arg Val
165 170 175
Glu Asp Met Glu Gln Gly Glu Pro Asp Asn Phe Gly Lys Arg Gly Pro
180 185 190
Gln Asp Phe Ala Leu Trp Lys Ala Ala Lys Pro Gly Glu Pro Ser Trp
195 200 205
Pro Thr Pro Trp Gly Asp Gly Arg Pro Gly Trp His Leu Glu Cys Ser
210 215 220
Ala Met Ala Thr Tyr Tyr Leu Gly Glu Gln Phe Asp Ile His Cys Gly
225 230 235 240
Gly Leu Asp Leu Gln Phe Pro His His Glu Asn Glu Ile Ala Gln Ala
245 250 255
His Ala Ala Gly Asp Lys Phe Ala Asn Tyr Trp Met His Asn His Trp
260 265 270
Val Thr Met Ala Gly Glu Lys Met Ser Lys Ser Leu Gly Asn Val Leu
275 280 285
Ala Val Pro Glu Met Leu Lys Gln Val Arg Pro Val Glu Leu Arg Tyr
290 295 300
Tyr Leu Gly Ser Ala His Tyr Arg Ser Val Leu Glu Tyr Ser Glu Ser
305 310 315 320
Ala Leu Ser Glu Ala Ala Val Gly Tyr Arg Arg Ile Glu Ser Phe Leu
325 330 335
Glu Arg Val Gly Asp Val Glu Val Gly Glu Trp Thr Pro Gly Phe Glu
340 345 350
Val Ala Met Asp Glu Asp Ile Ala Val Pro Lys Ala Leu Ala Glu Ile
355 360 365
His Asn Ala Val Arg Glu Gly Asn Ala Ala Leu Asp Lys Gly Asp Arg
370 375 380
Glu Ala Ala Glu Lys Leu Ala Ser Ser Val Arg Ala Met Thr Gly Val
385 390 395 400
Leu Gly Phe Asp Pro Val Glu Trp Gly Ser Asp Ala Gly Ala Asp Gly
405 410 415
Lys Ala Asp Lys Ala Leu Asp Val Leu Ile Ser Ser Glu Leu Glu Arg
420 425 430
Arg Ala Thr Ala Arg Ala Glu Lys Asn Trp Ala Val Ala Asp Glu Val
435 440 445
Arg Asp Arg Leu Ala Asp Ala Gly Ile Glu Val Val Asp Thr Ala Asp
450 455 460
Gly Ala Thr Trp Lys Leu Gln
465 470
<210> 11
<211> 1009
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(979)
<223> RXA00458
<400> 11
cacccctgaa aacctcctca actatcccgg agtgatcatc tccaccgttc aggagaaccc 60
atccgaaaca tggcggcaag tgaacatcta atctagaaac atg gca gga cga tac 115
Met Ala Gly Arg Tyr
1 5
gca cca tca cca agc ggc gac ctt cac ttt ggc aac ctc cgc aca gca 163
Ala Pro Ser Pro Ser Gly Asp Leu His Phe Gly Asn Leu Arg Thr Ala
10 15 20
ctg ctg gcc tgg ctg ttc gcg cgc tcc gaa gga aaa aaa ttc ctc atg 211
Leu Leu Ala Trp Leu Phe Ala Arg Ser Glu Gly Lys Lys Phe Leu Met
25 30 35
cgg gtc gaa gac atc gat gaa caa cgc tca tcc aag gaa tcc gcc gaa 259
Arg Val Glu Asp Ile Asp Glu Gln Arg Ser Ser Lys Glu Ser Ala Glu
40 45 50
agc caa ctc gca gac cta tcc gcc ctg ggt ctc gat tgg gat ggc gac 307
Ser Gln Leu Ala Asp Leu Ser Ala Leu Gly Leu Asp Trp Asp Gly Asp
55 60 65
gtc ctc tac caa tcc aca cgc tac gac gcc tac cgc gca gcc ctt gaa 355
Val Leu Tyr Gln Ser Thr Arg Tyr Asp Ala Tyr Arg Ala Ala Leu Glu
70 75 80 85
aaa cta gac acc tac gaa tgt tat tgc tcg cgc cgg gac atc caa gaa 403
Lys Leu Asp Thr Tyr Glu Cys Tyr Cys Ser Arg Arg Asp Ile Gln Glu
90 95 100
gcc tcg cgg gca ccc cat gtg gct ccg gga gtg tat ccg gga acg tgt 451
Ala Ser Arg Ala Pro His Val Ala Pro Gly Val Tyr Pro Gly Thr Cys
105 110 115
agg gga ttg aag gag gag gaa cgc gtc gaa aag cgt gca acc ttg gct 499
Arg Gly Leu Lys Glu Glu Glu Arg Val Glu Lys Arg Ala Thr Leu Ala
120 125 130
gcg caa aac cgg cac ccc gcc atc cgc ctg cgc gcg cag gta acc tcg 547
Ala Gln Asn Arg His Pro Ala Ile Arg Leu Arg Ala Gln Val Thr Ser
135 140 145
ttt gat ttt cac gac cga ctt cgc ggc cca caa act ggc ccc gta gac 595
Phe Asp Phe His Asp Arg Leu Arg Gly Pro Gln Thr Gly Pro Val Asp
150 155 160 165
gat ttc att ctg ctc cgc ggc ggg cag gaa ccc gga tgg gca tac aac 643
Asp Phe Ile Leu Leu Arg Gly Gly Gln Glu Pro Gly Trp Ala Tyr Asn
170 175 180
tta gct gtc gtc gtc gac gat gcc tac caa ggc gtt gac cag gta gtc 691
Leu Ala Val Val Val Asp Asp Ala Tyr Gln Gly Val Asp Gln Val Val
185 190 195
cgc ggc gac gac cta ctc gat tcc gcc gcg cgc caa gcc tac ctc ggc 739
Arg Gly Asp Asp Leu Leu Asp Ser Ala Ala Arg Gln Ala Tyr Leu Gly
200 205 210
tcg ctg ctg ggc acc ccc gcg ccc gaa tac att cac gtg ccg ctc gtg 787
Ser Leu Leu Gly Thr Pro Ala Pro Glu Tyr Ile His Val Pro Leu Val
215 220 225
ctc aac gcc cac ggc cag cgc ctc gcc aaa cgc gac ggg gca gtg acg 835
Leu Asn Ala His Gly Gln Arg Leu Ala Lys Arg Asp Gly Ala Val Thr
230 235 240 245
ctt aaa gaa atg ctt atc gac gcc ccc ctc cac acc att ttc tcc cgc 883
Leu Lys Glu Met Leu Ile Asp Ala Pro Leu His Thr Ile Phe Ser Arg
250 255 260
ctc gca tca tcg ctc ggc tac gaa ggg gta aat tcc gca ccc caa ttg 931
Leu Ala Ser Ser Leu Gly Tyr Glu Gly Val Asn Ser Ala Pro Gln Leu
265 270 275
ttg gaa att ttc gac ccc aca acc ctc agc cgg gag ccg ttt att tac 979
Leu Glu Ile Phe Asp Pro Thr Thr Leu Ser Arg Glu Pro Phe Ile Tyr
280 285 290
tgaggctcag agggaggggt cattccatct 1009
<210> 12
<211> 293
<212> PRT
<213> Corynebacterium glutamicum
<400> 12
Met Ala Gly Arg Tyr Ala Pro Ser Pro Ser Gly Asp Leu His Phe Gly
1 5 10 15
Asn Leu Arg Thr Ala Leu Leu Ala Trp Leu Phe Ala Arg Ser Glu Gly
20 25 30
Lys Lys Phe Leu Met Arg Val Glu Asp Ile Asp Glu Gln Arg Ser Ser
35 40 45
Lys Glu Ser Ala Glu Ser Gln Leu Ala Asp Leu Ser Ala Leu Gly Leu
50 55 60
Asp Trp Asp Gly Asp Val Leu Tyr Gln Ser Thr Arg Tyr Asp Ala Tyr
65 70 75 80
Arg Ala Ala Leu Glu Lys Leu Asp Thr Tyr Glu Cys Tyr Cys Ser Arg
85 90 95
Arg Asp Ile Gln Glu Ala Ser Arg Ala Pro His Val Ala Pro Gly Val
100 105 110
Tyr Pro Gly Thr Cys Arg Gly Leu Lys Glu Glu Glu Arg Val Glu Lys
115 120 125
Arg Ala Thr Leu Ala Ala Gln Asn Arg His Pro Ala Ile Arg Leu Arg
130 135 140
Ala Gln Val Thr Ser Phe Asp Phe His Asp Arg Leu Arg Gly Pro Gln
145 150 155 160
Thr Gly Pro Val Asp Asp Phe Ile Leu Leu Arg Gly Gly Gln Glu Pro
165 170 175
Gly Trp Ala Tyr Asn Leu Ala Val Val Val Asp Asp Ala Tyr Gln Gly
180 185 190
Val Asp Gln Val Val Arg Gly Asp Asp Leu Leu Asp Ser Ala Ala Arg
195 200 205
Gln Ala Tyr Leu Gly Ser Leu Leu Gly Thr Pro Ala Pro Glu Tyr Ile
210 215 220
His Val Pro Leu Val Leu Asn Ala His Gly Gln Arg Leu Ala Lys Arg
225 230 235 240
Asp Gly Ala Val Thr Leu Lys Glu Met Leu Ile Asp Ala Pro Leu His
245 250 255
Thr Ile Phe Ser Arg Leu Ala Ser Ser Leu Gly Tyr Glu Gly Val Asn
260 265 270
Ser Ala Pro Gln Leu Leu Glu Ile Phe Asp Pro Thr Thr Leu Ser Arg
275 280 285
Glu Pro Phe Ile Tyr
290
<210> 13
<211> 1744
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1714)
<223> RXA00493
<400> 13
cccgttacgg cggcaccgag atcaagttcg gtggcgtgga gtacttgctt ctctccgctc 60
gtgacatcct cgcaatcgtc gagaagtagg ggataagttc atg gca aag ctc att 115
Met Ala Lys Leu Ile
1 5
gct ttt gac cag gac gcc cgc gaa ggc att ctc cgg ggc gtt gac gct 163
Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu Arg Gly Val Asp Ala
10 15 20
ctg gca aac gct gtc aag gta acc ctc ggc cca cgc ggc cgt aac gtg 211
Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro Arg Gly Arg Asn Val
25 30 35
gtt ctt gat aag gca ttc ggc gga cct ctg gtc acc aac gac ggt gtc 259
Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val Thr Asn Asp Gly Val
40 45 50
acc att gcc cgc gac atc gac ctt gag gat cct ttt gag aac ctc ggt 307
Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro Phe Glu Asn Leu Gly
55 60 65
gcg cag ctg gtg aag tcc gtt gct gtt aag acc aac gac atc gct ggt 355
Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr Asn Asp Ile Ala Gly
70 75 80 85
gac ggc acc acg act gca act ctg ctt gct cag gca ctc att gct gaa 403
Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln Ala Leu Ile Ala Glu
90 95 100
ggc ctg cgc aac gtt gct gct ggc gca aac cca atg gag ctc aac aag 451
Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro Met Glu Leu Asn Lys
105 110 115
ggt att tct gca gct gca gaa aag acc ttg gaa gag ttg aag gca cgc 499
Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu Glu Leu Lys Ala Arg
120 125 130
gca acc gag gtg tct gac acc aag gaa atc gca aac gtc gct acc gtt 547
Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala Asn Val Ala Thr Val
135 140 145
tca tcc cgc gat gaa gtt gtc ggc gag atc gtt gct gca gcg atg gaa 595
Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val Ala Ala Ala Met Glu
150 155 160 165
aag gtt ggc aag gac ggt gtc gtc acc gtt gag gag tcc cag tcc atc 643
Lys Val Gly Lys Asp Gly Val Val Thr Val Glu Glu Ser Gln Ser Ile
170 175 180
gag act gct ctc gag gtc acc gaa ggt att tct ttc gac aag ggc tac 691
Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser Phe Asp Lys Gly Tyr
185 190 195
ctt tcc cct tat ttc atc aac gac aac gac act cag cag gct gtc ctg 739
Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr Gln Gln Ala Val Leu
200 205 210
gac aac cct gca gtg ctg ctt gtt cgc aac aag att tct tcc ctc cca 787
Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys Ile Ser Ser Leu Pro
215 220 225
gac ttc ctc cca ttg ctg gag aag gtt gtg gag tcc aac cgt cct ttg 835
Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu Ser Asn Arg Pro Leu
230 235 240 245
ctg atc atc gca gaa gac gtc gag ggc gag cct ttg cag acc ctg gtt 883
Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro Leu Gln Thr Leu Val
250 255 260
gtg aac tcc atc cgc aag acc atc aag gtc gtt gca gtg aag tcc cct 931
Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val Ala Val Lys Ser Pro
265 270 275
tac ttc ggt gac cga cgc aag gcg ttc atg gat gac ctg gct att gtc 979
Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp Asp Leu Ala Ile Val
280 285 290
acc aag gca act gtc gtg gat cca gaa gtg ggc atc aac ctc aac gaa 1027
Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly Ile Asn Leu Asn Glu
295 300 305
gct ggc gaa gaa gtt ttc ggt acc gca cgc cgc atc acc gtt tcc aag 1075
Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg Ile Thr Val Ser Lys
310 315 320 325
gac gaa acc atc atc gtt gat ggt gca ggt tcc gca gaa gac gtt gaa 1123
Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser Ala Glu Asp Val Glu
330 335 340
gca cgt cgc ggc cag atc cgt cgc gaa atc gcc aac acc gat tcc acc 1171
Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala Asn Thr Asp Ser Thr
345 350 355
tgg gat cgc gaa aag gca gaa gag cgt ttg gct aag ctc tcc ggt ggt 1219
Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala Lys Leu Ser Gly Gly
360 365 370
att gct gtc atc cgc gtt ggt gca gca act gaa acc gaa gtc aac gac 1267
Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu Thr Glu Val Asn Asp
375 380 385
cgc aag ctg cgt gtc gaa gat gcc atc aac gct gct cgc gca gca gca 1315
Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala Ala Arg Ala Ala Ala
390 395 400 405
caa gaa ggc gtt atc gct ggt ggc ggt tcc gct ttg gtt cag atc gct 1363
Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala Leu Val Gln Ile Ala
410 415 420
gag act ctg aag gct tac gcc gaa gag ttc gaa ggc gac cag aag gtc 1411
Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu Gly Asp Gln Lys Val
425 430 435
ggc gtt cgc gca ctg gct act gct ttg ggc aag cca gcg tac tgg atc 1459
Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys Pro Ala Tyr Trp Ile
440 445 450
gcc tcc aac gca ggt ctt gac ggc tct gtt gtt gtt gca cgc act gct 1507
Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val Val Ala Arg Thr Ala
455 460 465
gct ctg cca aac ggc gag ggc ttc aac gct gca act ttg gaa tac gga 1555
Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala Thr Leu Glu Tyr Gly
470 475 480 485
aac ctg atc aac gac ggt gtc atc gac cca gtc aag gtc acc cat tcc 1603
Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val Lys Val Thr His Ser
490 495 500
gca gta gtg aat gca acc tct gtt gca cgc atg gtt ctg acc act gag 1651
Ala Val Val Asn Ala Thr Ser Val Ala Arg Met Val Leu Thr Thr Glu
505 510 515
gct tct gtt gtt gag aag cct gca gaa gaa gca gcc gat gca cat gca 1699
Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala Ala Asp Ala His Ala
520 525 530
gga cat cat cac cac taaagttctg tgaaaaacac cgtggggcag 1744
Gly His His His His
535
<210> 14
<211> 538
<212> PRT
<213> Corynebacterium glutamicum
<400> 14
Met Ala Lys Leu Ile Ala Phe Asp Gln Asp Ala Arg Glu Gly Ile Leu
1 5 10 15
Arg Gly Val Asp Ala Leu Ala Asn Ala Val Lys Val Thr Leu Gly Pro
20 25 30
Arg Gly Arg Asn Val Val Leu Asp Lys Ala Phe Gly Gly Pro Leu Val
35 40 45
Thr Asn Asp Gly Val Thr Ile Ala Arg Asp Ile Asp Leu Glu Asp Pro
50 55 60
Phe Glu Asn Leu Gly Ala Gln Leu Val Lys Ser Val Ala Val Lys Thr
65 70 75 80
Asn Asp Ile Ala Gly Asp Gly Thr Thr Thr Ala Thr Leu Leu Ala Gln
85 90 95
Ala Leu Ile Ala Glu Gly Leu Arg Asn Val Ala Ala Gly Ala Asn Pro
100 105 110
Met Glu Leu Asn Lys Gly Ile Ser Ala Ala Ala Glu Lys Thr Leu Glu
115 120 125
Glu Leu Lys Ala Arg Ala Thr Glu Val Ser Asp Thr Lys Glu Ile Ala
130 135 140
Asn Val Ala Thr Val Ser Ser Arg Asp Glu Val Val Gly Glu Ile Val
145 150 155 160
Ala Ala Ala Met Glu Lys Val Gly Lys Asp Gly Val Val Thr Val Glu
165 170 175
Glu Ser Gln Ser Ile Glu Thr Ala Leu Glu Val Thr Glu Gly Ile Ser
180 185 190
Phe Asp Lys Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Asp Asn Asp Thr
195 200 205
Gln Gln Ala Val Leu Asp Asn Pro Ala Val Leu Leu Val Arg Asn Lys
210 215 220
Ile Ser Ser Leu Pro Asp Phe Leu Pro Leu Leu Glu Lys Val Val Glu
225 230 235 240
Ser Asn Arg Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Pro
245 250 255
Leu Gln Thr Leu Val Val Asn Ser Ile Arg Lys Thr Ile Lys Val Val
260 265 270
Ala Val Lys Ser Pro Tyr Phe Gly Asp Arg Arg Lys Ala Phe Met Asp
275 280 285
Asp Leu Ala Ile Val Thr Lys Ala Thr Val Val Asp Pro Glu Val Gly
290 295 300
Ile Asn Leu Asn Glu Ala Gly Glu Glu Val Phe Gly Thr Ala Arg Arg
305 310 315 320
Ile Thr Val Ser Lys Asp Glu Thr Ile Ile Val Asp Gly Ala Gly Ser
325 330 335
Ala Glu Asp Val Glu Ala Arg Arg Gly Gln Ile Arg Arg Glu Ile Ala
340 345 350
Asn Thr Asp Ser Thr Trp Asp Arg Glu Lys Ala Glu Glu Arg Leu Ala
355 360 365
Lys Leu Ser Gly Gly Ile Ala Val Ile Arg Val Gly Ala Ala Thr Glu
370 375 380
Thr Glu Val Asn Asp Arg Lys Leu Arg Val Glu Asp Ala Ile Asn Ala
385 390 395 400
Ala Arg Ala Ala Ala Gln Glu Gly Val Ile Ala Gly Gly Gly Ser Ala
405 410 415
Leu Val Gln Ile Ala Glu Thr Leu Lys Ala Tyr Ala Glu Glu Phe Glu
420 425 430
Gly Asp Gln Lys Val Gly Val Arg Ala Leu Ala Thr Ala Leu Gly Lys
435 440 445
Pro Ala Tyr Trp Ile Ala Ser Asn Ala Gly Leu Asp Gly Ser Val Val
450 455 460
Val Ala Arg Thr Ala Ala Leu Pro Asn Gly Glu Gly Phe Asn Ala Ala
465 470 475 480
Thr Leu Glu Tyr Gly Asn Leu Ile Asn Asp Gly Val Ile Asp Pro Val
485 490 495
Lys Val Thr His Ser Ala Val Val Asn Ala Thr Ser Val Ala Arg Met
500 505 510
Val Leu Thr Thr Glu Ala Ser Val Val Glu Lys Pro Ala Glu Glu Ala
515 520 525
Ala Asp Ala His Ala Gly His His His His
530 535
<210> 15
<211> 652
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(622)
<223> RXA00588
<400> 15
tcatacatct tggccccgga aaaccggggc caatcttatg gctcaagtcg ctagttagcc 60
gatgatccac ctctactgtt ccccaggagg gtaagtaatt atg gca agt gta gat 115
Met Ala Ser Val Asp
1 5
aag caa tac atc acc cca gaa acc aag gcc aag ctg gag gaa gag ctc 163
Lys Gln Tyr Ile Thr Pro Glu Thr Lys Ala Lys Leu Glu Glu Glu Leu
10 15 20
aac gcc ctc atc gca cac cgc cct gca gtt gct gcg gaa atc aat gag 211
Asn Ala Leu Ile Ala His Arg Pro Ala Val Ala Ala Glu Ile Asn Glu
25 30 35
cgc cgt gaa gaa ggc gac ctc aag gaa aac gct ggc tat gac gcc gct 259
Arg Arg Glu Glu Gly Asp Leu Lys Glu Asn Ala Gly Tyr Asp Ala Ala
40 45 50
cgt gaa atg cag gac cag gaa gag gcc cgc atc aag cag atc tct gag 307
Arg Glu Met Gln Asp Gln Glu Glu Ala Arg Ile Lys Gln Ile Ser Glu
55 60 65
ctg ctg gcc aac tcc acc act gag cgc gaa ggc atc atc gaa ggt gtc 355
Leu Leu Ala Asn Ser Thr Thr Glu Arg Glu Gly Ile Ile Glu Gly Val
70 75 80 85
gca aac gtt ggc tcc gtt gtt cac gtc tac tac gac ggc gac gag aac 403
Ala Asn Val Gly Ser Val Val His Val Tyr Tyr Asp Gly Asp Glu Asn
90 95 100
gac aag gaa acc ttc ctc atc ggt acc cgt gct ggc gct tcc gag aac 451
Asp Lys Glu Thr Phe Leu Ile Gly Thr Arg Ala Gly Ala Ser Glu Asn
105 110 115
cca gat ctt gag acc tac tct gag cag tcc cca ctc ggc gct gca att 499
Pro Asp Leu Glu Thr Tyr Ser Glu Gln Ser Pro Leu Gly Ala Ala Ile
120 125 130
ctc gga gct cag gaa ggc gac acc cgt cag tac acc gct cca aat ggt 547
Leu Gly Ala Gln Glu Gly Asp Thr Arg Gln Tyr Thr Ala Pro Asn Gly
135 140 145
tcc gtt atc tcc gta act gtt gtt tct gca gaa cca tac aac tca gca 595
Ser Val Ile Ser Val Thr Val Val Ser Ala Glu Pro Tyr Asn Ser Ala
150 155 160 165
aaa gcc gcg aca ctc cgc ggc aaa aac taaccaagga tttaaaagtc 642
Lys Ala Ala Thr Leu Arg Gly Lys Asn
170
ttcaaaatga 652
<210> 16
<211> 174
<212> PRT
<213> Corynebacterium glutamicum
<400> 16
Met Ala Ser Val Asp Lys Gln Tyr Ile Thr Pro Glu Thr Lys Ala Lys
1 5 10 15
Leu Glu Glu Glu Leu Asn Ala Leu Ile Ala His Arg Pro Ala Val Ala
20 25 30
Ala Glu Ile Asn Glu Arg Arg Glu Glu Gly Asp Leu Lys Glu Asn Ala
35 40 45
Gly Tyr Asp Ala Ala Arg Glu Met Gln Asp Gln Glu Glu Ala Arg Ile
50 55 60
Lys Gln Ile Ser Glu Leu Leu Ala Asn Ser Thr Thr Glu Arg Glu Gly
65 70 75 80
Ile Ile Glu Gly Val Ala Asn Val Gly Ser Val Val His Val Tyr Tyr
85 90 95
Asp Gly Asp Glu Asn Asp Lys Glu Thr Phe Leu Ile Gly Thr Arg Ala
100 105 110
Gly Ala Ser Glu Asn Pro Asp Leu Glu Thr Tyr Ser Glu Gln Ser Pro
115 120 125
Leu Gly Ala Ala Ile Leu Gly Ala Gln Glu Gly Asp Thr Arg Gln Tyr
130 135 140
Thr Ala Pro Asn Gly Ser Val Ile Ser Val Thr Val Val Ser Ala Glu
145 150 155 160
Pro Tyr Asn Ser Ala Lys Ala Ala Thr Leu Arg Gly Lys Asn
165 170
<210> 17
<211> 1012
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(982)
<223> RXA00669
<400> 17
tttactgcgg gcattttacg tatctgcacc ccgcccggct gcgctgagca gccgtaaagc 60
gtggggcgtg acgtcgaaaa gcaaaaaatg aaaggcagac atg gac aat tca acg 115
Met Asp Asn Ser Thr
1 5
gtg cga atc cgg ctg gat cta gcg tat gac ggc acg gat ttt cat ggc 163
Val Arg Ile Arg Leu Asp Leu Ala Tyr Asp Gly Thr Asp Phe His Gly
10 15 20
tgg gcg aag cag ggg acc agc gat cta cgc acc gtg caa aaa gtg ttg 211
Trp Ala Lys Gln Gly Thr Ser Asp Leu Arg Thr Val Gln Lys Val Leu
25 30 35
gaa gac aat ttg agc atg gtg ctg cgt gag act gtt gaa ttg act gtg 259
Glu Asp Asn Leu Ser Met Val Leu Arg Glu Thr Val Glu Leu Thr Val
40 45 50
gcc ggg cga acc gat gcg ggg gtg cat gcg gcg ggc cag gtg gcg cac 307
Ala Gly Arg Thr Asp Ala Gly Val His Ala Ala Gly Gln Val Ala His
55 60 65
ttt gat att ccg gca cac gct tta gag cag cgc agt att gat ggc gat 355
Phe Asp Ile Pro Ala His Ala Leu Glu Gln Arg Ser Ile Asp Gly Asp
70 75 80 85
cca agc aag ttg gtt cgg cgc ttg ggt cgg ttg ctg ccc gat gat att 403
Pro Ser Lys Leu Val Arg Arg Leu Gly Arg Leu Leu Pro Asp Asp Ile
90 95 100
cgg gtg cat ggc gta cgt ttt gcc gag ccc ggg ttt gat gcg cga ttt 451
Arg Val His Gly Val Arg Phe Ala Glu Pro Gly Phe Asp Ala Arg Phe
105 110 115
tcc gcg atg cgc agg cac tac gtt tat cgc att acg acg cat ccc gcc 499
Ser Ala Met Arg Arg His Tyr Val Tyr Arg Ile Thr Thr His Pro Ala
120 125 130
ggc gcg ctg cct acg cgc cgc cac gac acg gcg cag tgg cca aaa cct 547
Gly Ala Leu Pro Thr Arg Arg His Asp Thr Ala Gln Trp Pro Lys Pro
135 140 145
gtc gaa cta gag cgg atg caa tta gcc gcc gat gca ctg ctg ggg ctg 595
Val Glu Leu Glu Arg Met Gln Leu Ala Ala Asp Ala Leu Leu Gly Leu
150 155 160 165
cat gat ttt gtg gcg ttt tgc aaa gct aag cca cat gcg acg acg gtg 643
His Asp Phe Val Ala Phe Cys Lys Ala Lys Pro His Ala Thr Thr Val
170 175 180
cgt gaa cta caa aaa ttt gcg tgg aaa gac gtc tcc act gac atc gaa 691
Arg Glu Leu Gln Lys Phe Ala Trp Lys Asp Val Ser Thr Asp Ile Glu
185 190 195
ccg cag gtg tat gaa gca cac gtg gtg gcc gat gct ttt tgc tgg tcg 739
Pro Gln Val Tyr Glu Ala His Val Val Ala Asp Ala Phe Cys Trp Ser
200 205 210
atg gtg cgc tcg ctg gtc ggc tcc tgc atg gcc gtg ggc gaa gga cgc 787
Met Val Arg Ser Leu Val Gly Ser Cys Met Ala Val Gly Glu Gly Arg
215 220 225
cgc gga tca ggg ttt act gca gaa ttg ctt gat gca agc gaa cgc agc 835
Arg Gly Ser Gly Phe Thr Ala Glu Leu Leu Asp Ala Ser Glu Arg Ser
230 235 240 245
ccc atg gtt cca gta gca cct gcg aaa ggt ttg agc ttg gtt ggc gtg 883
Pro Met Val Pro Val Ala Pro Ala Lys Gly Leu Ser Leu Val Gly Val
250 255 260
gat tat cct tcc gct gat aag tta cag gaa aga gcg ctg gaa acc cga 931
Asp Tyr Pro Ser Ala Asp Lys Leu Gln Glu Arg Ala Leu Glu Thr Arg
265 270 275
gct gtt cgc gag ttt ccg gac gcg tcc gcg agc cta aaa cta gat gat 979
Ala Val Arg Glu Phe Pro Asp Ala Ser Ala Ser Leu Lys Leu Asp Asp
280 285 290
gag taaaagggac taaactcgtc tctcgtatct 1012
Glu
<210> 18
<211> 294
<212> PRT
<213> Corynebacterium glutamicum
<400> 18
Met Asp Asn Ser Thr Val Arg Ile Arg Leu Asp Leu Ala Tyr Asp Gly
1 5 10 15
Thr Asp Phe His Gly Trp Ala Lys Gln Gly Thr Ser Asp Leu Arg Thr
20 25 30
Val Gln Lys Val Leu Glu Asp Asn Leu Ser Met Val Leu Arg Glu Thr
35 40 45
Val Glu Leu Thr Val Ala Gly Arg Thr Asp Ala Gly Val His Ala Ala
50 55 60
Gly Gln Val Ala His Phe Asp Ile Pro Ala His Ala Leu Glu Gln Arg
65 70 75 80
Ser Ile Asp Gly Asp Pro Ser Lys Leu Val Arg Arg Leu Gly Arg Leu
85 90 95
Leu Pro Asp Asp Ile Arg Val His Gly Val Arg Phe Ala Glu Pro Gly
100 105 110
Phe Asp Ala Arg Phe Ser Ala Met Arg Arg His Tyr Val Tyr Arg Ile
115 120 125
Thr Thr His Pro Ala Gly Ala Leu Pro Thr Arg Arg His Asp Thr Ala
130 135 140
Gln Trp Pro Lys Pro Val Glu Leu Glu Arg Met Gln Leu Ala Ala Asp
145 150 155 160
Ala Leu Leu Gly Leu His Asp Phe Val Ala Phe Cys Lys Ala Lys Pro
165 170 175
His Ala Thr Thr Val Arg Glu Leu Gln Lys Phe Ala Trp Lys Asp Val
180 185 190
Ser Thr Asp Ile Glu Pro Gln Val Tyr Glu Ala His Val Val Ala Asp
195 200 205
Ala Phe Cys Trp Ser Met Val Arg Ser Leu Val Gly Ser Cys Met Ala
210 215 220
Val Gly Glu Gly Arg Arg Gly Ser Gly Phe Thr Ala Glu Leu Leu Asp
225 230 235 240
Ala Ser Glu Arg Ser Pro Met Val Pro Val Ala Pro Ala Lys Gly Leu
245 250 255
Ser Leu Val Gly Val Asp Tyr Pro Ser Ala Asp Lys Leu Gln Glu Arg
260 265 270
Ala Leu Glu Thr Arg Ala Val Arg Glu Phe Pro Asp Ala Ser Ala Ser
275 280 285
Leu Lys Leu Asp Asp Glu
290
<210> 19
<211> 3022
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2992)
<223> RXA01061
<400> 19
gcagacaaga ctgagcagtc cgacggcgat aagcagtggt ttccacataa ttcttcaagt 60
ctatctactt attgagggga ggaagaattg ccctccacac atg aga tgt ccc gtg 115
Met Arg Cys Pro Val
1 5
tac cta cta cac tgt tta acc atg act aac ccg agc gaa ggc acc act 163
Tyr Leu Leu His Cys Leu Thr Met Thr Asn Pro Ser Glu Gly Thr Thr
10 15 20
ccc ctg gcg ttc cgt tat acc ccg gaa ctc gcc aac aag atc gaa ggt 211
Pro Leu Ala Phe Arg Tyr Thr Pro Glu Leu Ala Asn Lys Ile Glu Gly
25 30 35
gag tgg cag aat tac tgg act gac aac ggc aca ttc aac gca ccc aac 259
Glu Trp Gln Asn Tyr Trp Thr Asp Asn Gly Thr Phe Asn Ala Pro Asn
40 45 50
cca gtg ggt gat tta gcg cct gcg gac ggt aaa gca ctt cct gag gac 307
Pro Val Gly Asp Leu Ala Pro Ala Asp Gly Lys Ala Leu Pro Glu Asp
55 60 65
aag ctc ttt gtc cag gat atg ttc ccg tac cca tcc gga gct ggc ctg 355
Lys Leu Phe Val Gln Asp Met Phe Pro Tyr Pro Ser Gly Ala Gly Leu
70 75 80 85
cac gta ggc cac cca ctc ggt tac atc gca acg gat gtt ttc gcc cgc 403
His Val Gly His Pro Leu Gly Tyr Ile Ala Thr Asp Val Phe Ala Arg
90 95 100
tac aac cgc atg ctg ggc aag aac gtt ctg cac acc ttg ggc tat gac 451
Tyr Asn Arg Met Leu Gly Lys Asn Val Leu His Thr Leu Gly Tyr Asp
105 110 115
gcc ttc gga ctg cca gca gag cag tac gcg atc caa acc ggt aca cac 499
Ala Phe Gly Leu Pro Ala Glu Gln Tyr Ala Ile Gln Thr Gly Thr His
120 125 130
cca cgc acc acc acc atg gcc aac att gag aac atg aag cgc cag ctc 547
Pro Arg Thr Thr Thr Met Ala Asn Ile Glu Asn Met Lys Arg Gln Leu
135 140 145
ggt gcg ctg ggt ctt ggc cat gat tcc cgt cgt gcg gtg gcc acc acg 595
Gly Ala Leu Gly Leu Gly His Asp Ser Arg Arg Ala Val Ala Thr Thr
150 155 160 165
gat cct gag ttc tac aag tgg act cag tgg atc ttc ctg cag att ttc 643
Asp Pro Glu Phe Tyr Lys Trp Thr Gln Trp Ile Phe Leu Gln Ile Phe
170 175 180
aat tcg tgg ttc gat gca gag cag cag aag gca cgt ccc atc agt gag 691
Asn Ser Trp Phe Asp Ala Glu Gln Gln Lys Ala Arg Pro Ile Ser Glu
185 190 195
ctg att ccg ttg ctg gag tcc ggc gag ctg aag act aag gac ggg gcg 739
Leu Ile Pro Leu Leu Glu Ser Gly Glu Leu Lys Thr Lys Asp Gly Ala
200 205 210
gat tac aac gcg ctg gga gac gtc gaa aag caa aaa gcg gtg gat gac 787
Asp Tyr Asn Ala Leu Gly Asp Val Glu Lys Gln Lys Ala Val Asp Asp
215 220 225
tac cgc ctt gtt tat cgc tcg aac tcc acc gtg aac tgg tgc cca ggc 835
Tyr Arg Leu Val Tyr Arg Ser Asn Ser Thr Val Asn Trp Cys Pro Gly
230 235 240 245
ttg ggc acc gtg ttg gca aac gag gaa gtg acc gcg gac ggc cgt tcc 883
Leu Gly Thr Val Leu Ala Asn Glu Glu Val Thr Ala Asp Gly Arg Ser
250 255 260
gag cgt ggc aat ttc cct gtt ttc cgt aag aat ttg tcc cag tgg atg 931
Glu Arg Gly Asn Phe Pro Val Phe Arg Lys Asn Leu Ser Gln Trp Met
265 270 275
atg cgc att acc gcg tac tcg gat cgt ctg atc gat gat ctg gag ctg 979
Met Arg Ile Thr Ala Tyr Ser Asp Arg Leu Ile Asp Asp Leu Glu Leu
280 285 290
ctc gat tgg act gag aag gtc aag tcc atg cag cgt aac tgg att ggc 1027
Leu Asp Trp Thr Glu Lys Val Lys Ser Met Gln Arg Asn Trp Ile Gly
295 300 305
cgt tcc cgc ggc gct gaa gtt gat ttc agt gca gag ggc gaa acc gtc 1075
Arg Ser Arg Gly Ala Glu Val Asp Phe Ser Ala Glu Gly Glu Thr Val
310 315 320 325
acc gtg ttt acc acc cgc cca gat act ctg ttc ggc gcg acc tac atg 1123
Thr Val Phe Thr Thr Arg Pro Asp Thr Leu Phe Gly Ala Thr Tyr Met
330 335 340
gtt ctt gca cct gag cat gag ctg gtc gac gtg ctg ctg gag aag gct 1171
Val Leu Ala Pro Glu His Glu Leu Val Asp Val Leu Leu Glu Lys Ala
345 350 355
ggt tcc tac gag ggc gtt gat gcc cgt tgg acc aat ggc cag gcg agc 1219
Gly Ser Tyr Glu Gly Val Asp Ala Arg Trp Thr Asn Gly Gln Ala Ser
360 365 370
cct gcg gaa gct gtc gct gca tac cgc gcc tcc atc gcc gcg aag tcc 1267
Pro Ala Glu Ala Val Ala Ala Tyr Arg Ala Ser Ile Ala Ala Lys Ser
375 380 385
gac ctg gag cgt cag gaa aac aag gaa aag acc ggc gtc ttc ctg ggc 1315
Asp Leu Glu Arg Gln Glu Asn Lys Glu Lys Thr Gly Val Phe Leu Gly
390 395 400 405
gtt tac gcg acc aac cca gtc aac ggc gat cag atc aca gtg ttc atc 1363
Val Tyr Ala Thr Asn Pro Val Asn Gly Asp Gln Ile Thr Val Phe Ile
410 415 420
gct gac tac gtt ctg acc ggc tac ggc acc ggc gcc atc atg gcg gtt 1411
Ala Asp Tyr Val Leu Thr Gly Tyr Gly Thr Gly Ala Ile Met Ala Val
425 430 435
cct gct cac gac gag cgc gac tac gaa ttc gcc acc gtt ttg ggt ctg 1459
Pro Ala His Asp Glu Arg Asp Tyr Glu Phe Ala Thr Val Leu Gly Leu
440 445 450
cct atc aag gaa gtt gtc gca ggt ggc aac atc gaa gag gct gct ttc 1507
Pro Ile Lys Glu Val Val Ala Gly Gly Asn Ile Glu Glu Ala Ala Phe
455 460 465
acc gaa tct ggc gaa gca gtc aac tct gcg aac gac aac ggc ctg gat 1555
Thr Glu Ser Gly Glu Ala Val Asn Ser Ala Asn Asp Asn Gly Leu Asp
470 475 480 485
atc aac ggc ctt gcc aag gat gag gct att gcc aag acc atc gaa tgg 1603
Ile Asn Gly Leu Ala Lys Asp Glu Ala Ile Ala Lys Thr Ile Glu Trp
490 495 500
ttg gaa gaa aag gaa ctt ggc cgc ggc acc atc cag tac aag ctg cgc 1651
Leu Glu Glu Lys Glu Leu Gly Arg Gly Thr Ile Gln Tyr Lys Leu Arg
505 510 515
gac tgg ctg ttc gct cgc cag cgt tac tgg ggc gag cct ttc cca atc 1699
Asp Trp Leu Phe Ala Arg Gln Arg Tyr Trp Gly Glu Pro Phe Pro Ile
520 525 530
gtc tac gac gaa aac ggc caa gca cat gct ctg cca gac tcc atg ctt 1747
Val Tyr Asp Glu Asn Gly Gln Ala His Ala Leu Pro Asp Ser Met Leu
535 540 545
cca gtc gag ctg cca gag gta gag gac tac aag cct gtc tcc ttc gac 1795
Pro Val Glu Leu Pro Glu Val Glu Asp Tyr Lys Pro Val Ser Phe Asp
550 555 560 565
cct gaa gac gca gac tcc gag cct tcc cca cca ctg gct aag gcc cgc 1843
Pro Glu Asp Ala Asp Ser Glu Pro Ser Pro Pro Leu Ala Lys Ala Arg
570 575 580
gaa tgg gtt gag gtg gaa ctc gat ctc ggc gat ggc aag aag aag tac 1891
Glu Trp Val Glu Val Glu Leu Asp Leu Gly Asp Gly Lys Lys Lys Tyr
585 590 595
acc cgc gac acc aac gtc atg cca cag tgg gca ggt tcc tcc tgg tac 1939
Thr Arg Asp Thr Asn Val Met Pro Gln Trp Ala Gly Ser Ser Trp Tyr
600 605 610
cag ctg cgc tac gtc gat cca agc aac gat gag cag ttc tgc aac atc 1987
Gln Leu Arg Tyr Val Asp Pro Ser Asn Asp Glu Gln Phe Cys Asn Ile
615 620 625
gaa aat gaa cgc tac tgg acc ggc cca cgc cca gaa acc cac gga cca 2035
Glu Asn Glu Arg Tyr Trp Thr Gly Pro Arg Pro Glu Thr His Gly Pro
630 635 640 645
aac gat cca ggc ggc gta gac ctc tac gtc ggt ggc gtc gag cac gca 2083
Asn Asp Pro Gly Gly Val Asp Leu Tyr Val Gly Gly Val Glu His Ala
650 655 660
gtt ctc cac ctg ctc tac gca cgt ttc tgg cac aag gtc ctc ttc gac 2131
Val Leu His Leu Leu Tyr Ala Arg Phe Trp His Lys Val Leu Phe Asp
665 670 675
ctg ggc cac gtc tcc tcc aag gag cca tac cgt cgc ctg tac aac cag 2179
Leu Gly His Val Ser Ser Lys Glu Pro Tyr Arg Arg Leu Tyr Asn Gln
680 685 690
ggc tac atc cag gcc ttc gcc tac acc gat tcc cgt ggc gtc tac gtg 2227
Gly Tyr Ile Gln Ala Phe Ala Tyr Thr Asp Ser Arg Gly Val Tyr Val
695 700 705
cct gcc gat gat gtc gaa gag aag gac gga aag ttc ttc tac cag ggc 2275
Pro Ala Asp Asp Val Glu Glu Lys Asp Gly Lys Phe Phe Tyr Gln Gly
710 715 720 725
gaa gaa gtc aac cag gaa tac gga aag atg ggc aag tcc ctg aag aac 2323
Glu Glu Val Asn Gln Glu Tyr Gly Lys Met Gly Lys Ser Leu Lys Asn
730 735 740
gcc gtt gcc cca gac gat atc tgc aac aac ttc ggt gct gac acc ctg 2371
Ala Val Ala Pro Asp Asp Ile Cys Asn Asn Phe Gly Ala Asp Thr Leu
745 750 755
cgc gtt tac gag atg gcc atg gga cct ttg gac acc tcc cgt cca tgg 2419
Arg Val Tyr Glu Met Ala Met Gly Pro Leu Asp Thr Ser Arg Pro Trp
760 765 770
gca acc aag gac gtc gtc ggt gcg cag cgc ttc ctc cag cgt ctg tgg 2467
Ala Thr Lys Asp Val Val Gly Ala Gln Arg Phe Leu Gln Arg Leu Trp
775 780 785
cgt ctc gtc gtc gat gaa aac acc ggc gaa gtg ctc act cgc gat gaa 2515
Arg Leu Val Val Asp Glu Asn Thr Gly Glu Val Leu Thr Arg Asp Glu
790 795 800 805
gtc ctc acc gac gat gac aac aag caa ctg cac cgc acc atc gca ggc 2563
Val Leu Thr Asp Asp Asp Asn Lys Gln Leu His Arg Thr Ile Ala Gly
810 815 820
gtc cgc gac gac tac acc aac ttg cgc gtt aac acc gtg gtt gcc aag 2611
Val Arg Asp Asp Tyr Thr Asn Leu Arg Val Asn Thr Val Val Ala Lys
825 830 835
ctc atc gaa tac gtc aac tac ctg acc aaa aca tac cca gac acc atc 2659
Leu Ile Glu Tyr Val Asn Tyr Leu Thr Lys Thr Tyr Pro Asp Thr Ile
840 845 850
cca gct ggc gca gtc ctg cca ctg atc gtc atg gtc tcc cct atc gca 2707
Pro Ala Gly Ala Val Leu Pro Leu Ile Val Met Val Ser Pro Ile Ala
855 860 865
cca cac atc gcg gag gaa ctc tgg aag aag ctc ggc cac gac gac acc 2755
Pro His Ile Ala Glu Glu Leu Trp Lys Lys Leu Gly His Asp Asp Thr
870 875 880 885
gtc acc tac gaa cca ttc ccc acc ttt gag gaa aaa tgg ctc acc gac 2803
Val Thr Tyr Glu Pro Phe Pro Thr Phe Glu Glu Lys Trp Leu Thr Asp
890 895 900
gat gaa atc gaa ctg cca gtc cag gtc aac ggc aag gtc cgc ggt cgc 2851
Asp Glu Ile Glu Leu Pro Val Gln Val Asn Gly Lys Val Arg Gly Arg
905 910 915
atc acc gtt gca gcc gac gcc agc cag gag cag gtc atc gag gca gcg 2899
Ile Thr Val Ala Ala Asp Ala Ser Gln Glu Gln Val Ile Glu Ala Ala
920 925 930
ctt gcc gac gag aag gtg cag gag caa atc tcc ggc aag aac ctg atc 2947
Leu Ala Asp Glu Lys Val Gln Glu Gln Ile Ser Gly Lys Asn Leu Ile
935 940 945
aag cag atc gtt gtt cca gga cgc atg gtt aac ctt gtg gtg aag 2992
Lys Gln Ile Val Val Pro Gly Arg Met Val Asn Leu Val Val Lys
950 955 960
taatccccct cggtttagat tcccctagaa 3022
<210> 20
<211> 964
<212> PRT
<213> Corynebacterium glutamicum
<400> 20
Met Arg Cys Pro Val Tyr Leu Leu His Cys Leu Thr Met Thr Asn Pro
1 5 10 15
Ser Glu Gly Thr Thr Pro Leu Ala Phe Arg Tyr Thr Pro Glu Leu Ala
20 25 30
Asn Lys Ile Glu Gly Glu Trp Gln Asn Tyr Trp Thr Asp Asn Gly Thr
35 40 45
Phe Asn Ala Pro Asn Pro Val Gly Asp Leu Ala Pro Ala Asp Gly Lys
50 55 60
Ala Leu Pro Glu Asp Lys Leu Phe Val Gln Asp Met Phe Pro Tyr Pro
65 70 75 80
Ser Gly Ala Gly Leu His Val Gly His Pro Leu Gly Tyr Ile Ala Thr
85 90 95
Asp Val Phe Ala Arg Tyr Asn Arg Met Leu Gly Lys Asn Val Leu His
100 105 110
Thr Leu Gly Tyr Asp Ala Phe Gly Leu Pro Ala Glu Gln Tyr Ala Ile
115 120 125
Gln Thr Gly Thr His Pro Arg Thr Thr Thr Met Ala Asn Ile Glu Asn
130 135 140
Met Lys Arg Gln Leu Gly Ala Leu Gly Leu Gly His Asp Ser Arg Arg
145 150 155 160
Ala Val Ala Thr Thr Asp Pro Glu Phe Tyr Lys Trp Thr Gln Trp Ile
165 170 175
Phe Leu Gln Ile Phe Asn Ser Trp Phe Asp Ala Glu Gln Gln Lys Ala
180 185 190
Arg Pro Ile Ser Glu Leu Ile Pro Leu Leu Glu Ser Gly Glu Leu Lys
195 200 205
Thr Lys Asp Gly Ala Asp Tyr Asn Ala Leu Gly Asp Val Glu Lys Gln
210 215 220
Lys Ala Val Asp Asp Tyr Arg Leu Val Tyr Arg Ser Asn Ser Thr Val
225 230 235 240
Asn Trp Cys Pro Gly Leu Gly Thr Val Leu Ala Asn Glu Glu Val Thr
245 250 255
Ala Asp Gly Arg Ser Glu Arg Gly Asn Phe Pro Val Phe Arg Lys Asn
260 265 270
Leu Ser Gln Trp Met Met Arg Ile Thr Ala Tyr Ser Asp Arg Leu Ile
275 280 285
Asp Asp Leu Glu Leu Leu Asp Trp Thr Glu Lys Val Lys Ser Met Gln
290 295 300
Arg Asn Trp Ile Gly Arg Ser Arg Gly Ala Glu Val Asp Phe Ser Ala
305 310 315 320
Glu Gly Glu Thr Val Thr Val Phe Thr Thr Arg Pro Asp Thr Leu Phe
325 330 335
Gly Ala Thr Tyr Met Val Leu Ala Pro Glu His Glu Leu Val Asp Val
340 345 350
Leu Leu Glu Lys Ala Gly Ser Tyr Glu Gly Val Asp Ala Arg Trp Thr
355 360 365
Asn Gly Gln Ala Ser Pro Ala Glu Ala Val Ala Ala Tyr Arg Ala Ser
370 375 380
Ile Ala Ala Lys Ser Asp Leu Glu Arg Gln Glu Asn Lys Glu Lys Thr
385 390 395 400
Gly Val Phe Leu Gly Val Tyr Ala Thr Asn Pro Val Asn Gly Asp Gln
405 410 415
Ile Thr Val Phe Ile Ala Asp Tyr Val Leu Thr Gly Tyr Gly Thr Gly
420 425 430
Ala Ile Met Ala Val Pro Ala His Asp Glu Arg Asp Tyr Glu Phe Ala
435 440 445
Thr Val Leu Gly Leu Pro Ile Lys Glu Val Val Ala Gly Gly Asn Ile
450 455 460
Glu Glu Ala Ala Phe Thr Glu Ser Gly Glu Ala Val Asn Ser Ala Asn
465 470 475 480
Asp Asn Gly Leu Asp Ile Asn Gly Leu Ala Lys Asp Glu Ala Ile Ala
485 490 495
Lys Thr Ile Glu Trp Leu Glu Glu Lys Glu Leu Gly Arg Gly Thr Ile
500 505 510
Gln Tyr Lys Leu Arg Asp Trp Leu Phe Ala Arg Gln Arg Tyr Trp Gly
515 520 525
Glu Pro Phe Pro Ile Val Tyr Asp Glu Asn Gly Gln Ala His Ala Leu
530 535 540
Pro Asp Ser Met Leu Pro Val Glu Leu Pro Glu Val Glu Asp Tyr Lys
545 550 555 560
Pro Val Ser Phe Asp Pro Glu Asp Ala Asp Ser Glu Pro Ser Pro Pro
565 570 575
Leu Ala Lys Ala Arg Glu Trp Val Glu Val Glu Leu Asp Leu Gly Asp
580 585 590
Gly Lys Lys Lys Tyr Thr Arg Asp Thr Asn Val Met Pro Gln Trp Ala
595 600 605
Gly Ser Ser Trp Tyr Gln Leu Arg Tyr Val Asp Pro Ser Asn Asp Glu
610 615 620
Gln Phe Cys Asn Ile Glu Asn Glu Arg Tyr Trp Thr Gly Pro Arg Pro
625 630 635 640
Glu Thr His Gly Pro Asn Asp Pro Gly Gly Val Asp Leu Tyr Val Gly
645 650 655
Gly Val Glu His Ala Val Leu His Leu Leu Tyr Ala Arg Phe Trp His
660 665 670
Lys Val Leu Phe Asp Leu Gly His Val Ser Ser Lys Glu Pro Tyr Arg
675 680 685
Arg Leu Tyr Asn Gln Gly Tyr Ile Gln Ala Phe Ala Tyr Thr Asp Ser
690 695 700
Arg Gly Val Tyr Val Pro Ala Asp Asp Val Glu Glu Lys Asp Gly Lys
705 710 715 720
Phe Phe Tyr Gln Gly Glu Glu Val Asn Gln Glu Tyr Gly Lys Met Gly
725 730 735
Lys Ser Leu Lys Asn Ala Val Ala Pro Asp Asp Ile Cys Asn Asn Phe
740 745 750
Gly Ala Asp Thr Leu Arg Val Tyr Glu Met Ala Met Gly Pro Leu Asp
755 760 765
Thr Ser Arg Pro Trp Ala Thr Lys Asp Val Val Gly Ala Gln Arg Phe
770 775 780
Leu Gln Arg Leu Trp Arg Leu Val Val Asp Glu Asn Thr Gly Glu Val
785 790 795 800
Leu Thr Arg Asp Glu Val Leu Thr Asp Asp Asp Asn Lys Gln Leu His
805 810 815
Arg Thr Ile Ala Gly Val Arg Asp Asp Tyr Thr Asn Leu Arg Val Asn
820 825 830
Thr Val Val Ala Lys Leu Ile Glu Tyr Val Asn Tyr Leu Thr Lys Thr
835 840 845
Tyr Pro Asp Thr Ile Pro Ala Gly Ala Val Leu Pro Leu Ile Val Met
850 855 860
Val Ser Pro Ile Ala Pro His Ile Ala Glu Glu Leu Trp Lys Lys Leu
865 870 875 880
Gly His Asp Asp Thr Val Thr Tyr Glu Pro Phe Pro Thr Phe Glu Glu
885 890 895
Lys Trp Leu Thr Asp Asp Glu Ile Glu Leu Pro Val Gln Val Asn Gly
900 905 910
Lys Val Arg Gly Arg Ile Thr Val Ala Ala Asp Ala Ser Gln Glu Gln
915 920 925
Val Ile Glu Ala Ala Leu Ala Asp Glu Lys Val Gln Glu Gln Ile Ser
930 935 940
Gly Lys Asn Leu Ile Lys Gln Ile Val Val Pro Gly Arg Met Val Asn
945 950 955 960
Leu Val Val Lys
<210> 21
<211> 2248
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2218)
<223> RXA01277
<400> 21
gaccagccga atctacattc cttattctgc tggcgttaca attcagggcc aaacccgtat 60
gatgaaaaag acaccgggga aatcggagtg cgcgtagatt ttg aaa acg gcc ggt 115
Leu Lys Thr Ala Gly
1 5
act act cgg ttc acg ttt acg tcg gct gat cca att gga ggc gcc ctc 163
Thr Thr Arg Phe Thr Phe Thr Ser Ala Asp Pro Ile Gly Gly Ala Leu
10 15 20
gga agc cgc ctt aaa aaa cct gcc ggt caa aag atc act aac ctg aac 211
Gly Ser Arg Leu Lys Lys Pro Ala Gly Gln Lys Ile Thr Asn Leu Asn
25 30 35
ttc atg act gat tac acg ttc ctc gaa gac att gac acc ccg gaa gcg 259
Phe Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala
40 45 50
ctc gcg tgg gcg gaa aaa tgg tcg ggg gaa agc gtc gaa aag cta aaa 307
Leu Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser Val Glu Lys Leu Lys
55 60 65
agc cca gcc aag gac gcc ctg gaa gcc agg ctg ctg gct gcg ttg gac 355
Ser Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp
70 75 80 85
acc gat gat cgc att gcc tac gtg agc cgg cgc ggt gag aag ctg tac 403
Thr Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr
90 95 100
aac ttt tgg cgg gac gcg cag cat ccg cgt gga gtg tgg cgc acg acc 451
Asn Phe Trp Arg Asp Ala Gln His Pro Arg Gly Val Trp Arg Thr Thr
105 110 115
acg ttg gag tcg tat gaa agt gac cag ccg gag tgg gac gtg ctc att 499
Thr Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu Trp Asp Val Leu Ile
120 125 130
gat gtg gat gcg ttg gcg gag gat gag ggc gaa aac tgg gta tgg aag 547
Asp Val Asp Ala Leu Ala Glu Asp Glu Gly Glu Asn Trp Val Trp Lys
135 140 145
ggc gcg gtt gtg cgc tcg ccg gag ttt gat cgg gcg ttg gtg aag ttc 595
Gly Ala Val Val Arg Ser Pro Glu Phe Asp Arg Ala Leu Val Lys Phe
150 155 160 165
tcg cgg ggc ggg gct gat gcg acg gtg att agg gag ttt gat ctg gcc 643
Ser Arg Gly Gly Ala Asp Ala Thr Val Ile Arg Glu Phe Asp Leu Ala
170 175 180
acg gct gct ttc gtg gat gat tcg ccg ttt gaa ttg gag gag gcg aag 691
Thr Ala Ala Phe Val Asp Asp Ser Pro Phe Glu Leu Glu Glu Ala Lys
185 190 195
tcc gat gtc acg tgg gtt gat ctg gat acg ttg ctg gtg ggc acg gat 739
Ser Asp Val Thr Trp Val Asp Leu Asp Thr Leu Leu Val Gly Thr Asp
200 205 210
acc ggc gag ggg tca ctg acg gat tct ggg tac ccg gcg cgg gtg ctc 787
Thr Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu
215 220 225
acg tgg aag cgt ggg act ccg ctt gag cag gcg gag ttg ttc ttt gag 835
Thr Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu
230 235 240 245
ggg tcg cgt cag gat gtg gcg act cat gcg tgg cgg gat tca aca cct 883
Gly Ser Arg Gln Asp Val Ala Thr His Ala Trp Arg Asp Ser Thr Pro
250 255 260
ggt ttt gag cgg acg ttt gtg tca agg tcg ttg gat ttc tat aat tcg 931
Gly Phe Glu Arg Thr Phe Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser
265 270 275
gag acg tcg ctg gaa acc gag ggt ggc ctg gtc aag ctt gat gtg ccg 979
Glu Thr Ser Leu Glu Thr Glu Gly Gly Leu Val Lys Leu Asp Val Pro
280 285 290
acc gat tgc gat gtc att gtg aag aag cag tgg att ttt gtg agt cct 1027
Thr Asp Cys Asp Val Ile Val Lys Lys Gln Trp Ile Phe Val Ser Pro
295 300 305
cgg acg gat ttc gct ggg att cca gca ggt ggc ttg gga gtg ctg ctg 1075
Arg Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly Leu Gly Val Leu Leu
310 315 320 325
tta aag gag ttc ctt gag ggc ggg cgc gat ttt cag cct gtg ttt acg 1123
Leu Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe Gln Pro Val Phe Thr
330 335 340
cct act gag tcg acg tcg ctg cag gga ttg gcc acg aca aag aat ttc 1171
Pro Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe
345 350 355
ctg gtt tta acg ctc ctt aat aat gtc tcc aca gaa atc gtc aca gtg 1219
Leu Val Leu Thr Leu Leu Asn Asn Val Ser Thr Glu Ile Val Thr Val
360 365 370
ccg ctc aat gat ccg aca acg gag cat gaa cac att gac ctc cca gag 1267
Pro Leu Asn Asp Pro Thr Thr Glu His Glu His Ile Asp Leu Pro Glu
375 380 385
cat gtc acc gcg cat gtg gtt gct acc tcc ccg ttg gat ggc gat gaa 1315
His Val Thr Ala His Val Val Ala Thr Ser Pro Leu Asp Gly Asp Glu
390 395 400 405
att tgg gtg cag gca gcg agt ttc acc gaa gcg cca acg ttg ctg cgt 1363
Ile Trp Val Gln Ala Ala Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg
410 415 420
gcg gag ctg cct ggt gcg ctt gag gct gtg aag aag gcg ccg ttg cag 1411
Ala Glu Leu Pro Gly Ala Leu Glu Ala Val Lys Lys Ala Pro Leu Gln
425 430 435
ttt gaa aat gct ggt cag gag act cgt cag cat tgg gca acc tcg gcg 1459
Phe Glu Asn Ala Gly Gln Glu Thr Arg Gln His Trp Ala Thr Ser Ala
440 445 450
gat gga acg aag att ccg tac ttt att aca gga gcc ttc gag gag gaa 1507
Asp Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly Ala Phe Glu Glu Glu
455 460 465
cca caa aac acc ctg gtc cac gcc tac ggc ggc ttc gag gtt tcc ctt 1555
Pro Gln Asn Thr Leu Val His Ala Tyr Gly Gly Phe Glu Val Ser Leu
470 475 480 485
acc cca agc cac tcc ccg acc cgc ggc atc gca tgg ttg gaa aag ggc 1603
Thr Pro Ser His Ser Pro Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly
490 495 500
tac tac ttt gtg gaa gcc aac ctg cgt ggt ggc ggt gaa ttc ggt ccg 1651
Tyr Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro
505 510 515
gaa tgg cat tcg cag gca acc aag ctg aac cgc atg aag gtg tgg gag 1699
Glu Trp His Ser Gln Ala Thr Lys Leu Asn Arg Met Lys Val Trp Glu
520 525 530
gat cac cgc gcg gtg ctc gcc gac ctt gtg gag cgc ggc tac gca acg 1747
Asp His Arg Ala Val Leu Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr
535 540 545
ccg gag cag att gcg att cgt ggc gga tcc aac ggt ggt ttg ctg aca 1795
Pro Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr
550 555 560 565
agt ggc gcg tta act cag tac cca gaa gca ttc ggt gcg gca gtt gtg 1843
Ser Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe Gly Ala Ala Val Val
570 575 580
cag gtg ccg ttg gct gat atg ttg cgc tat cac acc tgg tca gcg ggt 1891
Gln Val Pro Leu Ala Asp Met Leu Arg Tyr His Thr Trp Ser Ala Gly
585 590 595
gct tcg tgg atg gcg gag tac ggc aac cct gac gat ccg gag gaa cgg 1939
Ala Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp Asp Pro Glu Glu Arg
600 605 610
gcg gtg att gag cag tac tcg ccg gtg cag gcg gtg gtg ggc gtc gag 1987
Ala Val Ile Glu Gln Tyr Ser Pro Val Gln Ala Val Val Gly Val Glu
615 620 625
aag cga att tat cca ccc gca ttg gtg acg acc tca acc cgg gac gac 2035
Lys Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr Ser Thr Arg Asp Asp
630 635 640 645
cgc gtc cac ccc gcg cac gcg cgc ctt ttt gct caa gct ttg ctt gat 2083
Arg Val His Pro Ala His Ala Arg Leu Phe Ala Gln Ala Leu Leu Asp
650 655 660
gcg ggc cag gcc gtg gat tac tac gaa aac acc gag ggc ggc cat gcc 2131
Ala Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr Glu Gly Gly His Ala
665 670 675
ggc gcg gcg gat aac aag cag acc gcg ttt gtg gaa tcg ctg atc tac 2179
Gly Ala Ala Asp Asn Lys Gln Thr Ala Phe Val Glu Ser Leu Ile Tyr
680 685 690
acc tgg atc gag aag act ttg gat cag cag ggt agc att taatacctat 2228
Thr Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly Ser Ile
695 700 705
gattatgcga aggctgcgct 2248
<210> 22
<211> 706
<212> PRT
<213> Corynebacterium glutamicum
<400> 22
Leu Lys Thr Ala Gly Thr Thr Arg Phe Thr Phe Thr Ser Ala Asp Pro
1 5 10 15
Ile Gly Gly Ala Leu Gly Ser Arg Leu Lys Lys Pro Ala Gly Gln Lys
20 25 30
Ile Thr Asn Leu Asn Phe Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile
35 40 45
Asp Thr Pro Glu Ala Leu Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser
50 55 60
Val Glu Lys Leu Lys Ser Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu
65 70 75 80
Leu Ala Ala Leu Asp Thr Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg
85 90 95
Gly Glu Lys Leu Tyr Asn Phe Trp Arg Asp Ala Gln His Pro Arg Gly
100 105 110
Val Trp Arg Thr Thr Thr Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu
115 120 125
Trp Asp Val Leu Ile Asp Val Asp Ala Leu Ala Glu Asp Glu Gly Glu
130 135 140
Asn Trp Val Trp Lys Gly Ala Val Val Arg Ser Pro Glu Phe Asp Arg
145 150 155 160
Ala Leu Val Lys Phe Ser Arg Gly Gly Ala Asp Ala Thr Val Ile Arg
165 170 175
Glu Phe Asp Leu Ala Thr Ala Ala Phe Val Asp Asp Ser Pro Phe Glu
180 185 190
Leu Glu Glu Ala Lys Ser Asp Val Thr Trp Val Asp Leu Asp Thr Leu
195 200 205
Leu Val Gly Thr Asp Thr Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr
210 215 220
Pro Ala Arg Val Leu Thr Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala
225 230 235 240
Glu Leu Phe Phe Glu Gly Ser Arg Gln Asp Val Ala Thr His Ala Trp
245 250 255
Arg Asp Ser Thr Pro Gly Phe Glu Arg Thr Phe Val Ser Arg Ser Leu
260 265 270
Asp Phe Tyr Asn Ser Glu Thr Ser Leu Glu Thr Glu Gly Gly Leu Val
275 280 285
Lys Leu Asp Val Pro Thr Asp Cys Asp Val Ile Val Lys Lys Gln Trp
290 295 300
Ile Phe Val Ser Pro Arg Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly
305 310 315 320
Leu Gly Val Leu Leu Leu Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe
325 330 335
Gln Pro Val Phe Thr Pro Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala
340 345 350
Thr Thr Lys Asn Phe Leu Val Leu Thr Leu Leu Asn Asn Val Ser Thr
355 360 365
Glu Ile Val Thr Val Pro Leu Asn Asp Pro Thr Thr Glu His Glu His
370 375 380
Ile Asp Leu Pro Glu His Val Thr Ala His Val Val Ala Thr Ser Pro
385 390 395 400
Leu Asp Gly Asp Glu Ile Trp Val Gln Ala Ala Ser Phe Thr Glu Ala
405 410 415
Pro Thr Leu Leu Arg Ala Glu Leu Pro Gly Ala Leu Glu Ala Val Lys
420 425 430
Lys Ala Pro Leu Gln Phe Glu Asn Ala Gly Gln Glu Thr Arg Gln His
435 440 445
Trp Ala Thr Ser Ala Asp Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly
450 455 460
Ala Phe Glu Glu Glu Pro Gln Asn Thr Leu Val His Ala Tyr Gly Gly
465 470 475 480
Phe Glu Val Ser Leu Thr Pro Ser His Ser Pro Thr Arg Gly Ile Ala
485 490 495
Trp Leu Glu Lys Gly Tyr Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly
500 505 510
Gly Glu Phe Gly Pro Glu Trp His Ser Gln Ala Thr Lys Leu Asn Arg
515 520 525
Met Lys Val Trp Glu Asp His Arg Ala Val Leu Ala Asp Leu Val Glu
530 535 540
Arg Gly Tyr Ala Thr Pro Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn
545 550 555 560
Gly Gly Leu Leu Thr Ser Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe
565 570 575
Gly Ala Ala Val Val Gln Val Pro Leu Ala Asp Met Leu Arg Tyr His
580 585 590
Thr Trp Ser Ala Gly Ala Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp
595 600 605
Asp Pro Glu Glu Arg Ala Val Ile Glu Gln Tyr Ser Pro Val Gln Ala
610 615 620
Val Val Gly Val Glu Lys Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr
625 630 635 640
Ser Thr Arg Asp Asp Arg Val His Pro Ala His Ala Arg Leu Phe Ala
645 650 655
Gln Ala Leu Leu Asp Ala Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr
660 665 670
Glu Gly Gly His Ala Gly Ala Ala Asp Asn Lys Gln Thr Ala Phe Val
675 680 685
Glu Ser Leu Ile Tyr Thr Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly
690 695 700
Ser Ile
705
<210> 23
<211> 2257
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2227)
<223> RXA01278
<400> 23
ttatccgtag gtgacaaact ttttaatact tgggtatctg tcatggatac cccggtaata 60
aataagtgaa ttaccgtaac caacaagttg gggtaccact gtg gca caa gaa gtg 115
Val Ala Gln Glu Val
1 5
ctt aag gat cta aac aag gtc cgc aac atc ggc atc atg gcg cac atc 163
Leu Lys Asp Leu Asn Lys Val Arg Asn Ile Gly Ile Met Ala His Ile
10 15 20
gat gct ggt aag acc acg acc acc gaa cgc atc ctc ttc tac acc ggc 211
Asp Ala Gly Lys Thr Thr Thr Thr Glu Arg Ile Leu Phe Tyr Thr Gly
25 30 35
atc aac cgt aag gtc ggt gag acc cac gac ggt ggc gca acc acc gac 259
Ile Asn Arg Lys Val Gly Glu Thr His Asp Gly Gly Ala Thr Thr Asp
40 45 50
tgg atg gag cag gag aag gaa cgc ggc atc acc att acc tcc gcc gcg 307
Trp Met Glu Gln Glu Lys Glu Arg Gly Ile Thr Ile Thr Ser Ala Ala
55 60 65
gtt acc tgt ttc tgg gat aac aac cag gtc aac atc att gac acc cct 355
Val Thr Cys Phe Trp Asp Asn Asn Gln Val Asn Ile Ile Asp Thr Pro
70 75 80 85
ggc cac gtt gac ttc acc gtt gag gtt gag cgt tcc ctc cgc gtg ctt 403
Gly His Val Asp Phe Thr Val Glu Val Glu Arg Ser Leu Arg Val Leu
90 95 100
gac ggc gca gtt gct gtg ttc gac ggc aag gaa ggc gtt gag cca cag 451
Asp Gly Ala Val Ala Val Phe Asp Gly Lys Glu Gly Val Glu Pro Gln
105 110 115
tct gag cag gtt tgg cgt cag gct acc aag tac gac gtt cca cgt atc 499
Ser Glu Gln Val Trp Arg Gln Ala Thr Lys Tyr Asp Val Pro Arg Ile
120 125 130
tgc ttc gtg aac aag atg gac aag ctc ggt gct gac ttc tac ttc acc 547
Cys Phe Val Asn Lys Met Asp Lys Leu Gly Ala Asp Phe Tyr Phe Thr
135 140 145
gtt ggc acc atc gag gac cgc ctg ggt gca aag cca ttg gtt atg cag 595
Val Gly Thr Ile Glu Asp Arg Leu Gly Ala Lys Pro Leu Val Met Gln
150 155 160 165
ctc cca atc ggt gct gag gac aac ttc gac ggc gtc atc gac ctt ctt 643
Leu Pro Ile Gly Ala Glu Asp Asn Phe Asp Gly Val Ile Asp Leu Leu
170 175 180
gaa atg aag gca ctg acc tgg cgt gga gtt acc cca att ggt acc gaa 691
Glu Met Lys Ala Leu Thr Trp Arg Gly Val Thr Pro Ile Gly Thr Glu
185 190 195
gct acc gtt gag gag atc cca gca gag ctc gca gac cgc gca gct gag 739
Ala Thr Val Glu Glu Ile Pro Ala Glu Leu Ala Asp Arg Ala Ala Glu
200 205 210
tac cgt gag aag ctt ctc gag acc gtt gca gag tcc gac gaa gag ctc 787
Tyr Arg Glu Lys Leu Leu Glu Thr Val Ala Glu Ser Asp Glu Glu Leu
215 220 225
atg gag aag tac ttc ggt ggc gaa gag ctc agc atc gct gag atc aag 835
Met Glu Lys Tyr Phe Gly Gly Glu Glu Leu Ser Ile Ala Glu Ile Lys
230 235 240 245
gca gct atc cgt aag atg gtt gtt aac tct gag atc tac cct gtt tac 883
Ala Ala Ile Arg Lys Met Val Val Asn Ser Glu Ile Tyr Pro Val Tyr
250 255 260
tgt ggc acc gcc tac aag aac aag ggc atc cag cca ctg ctc gac gca 931
Cys Gly Thr Ala Tyr Lys Asn Lys Gly Ile Gln Pro Leu Leu Asp Ala
265 270 275
gtc gtt gac ttc ctg cct tcc cca ctg gat ctc ggc gag acc aag ggc 979
Val Val Asp Phe Leu Pro Ser Pro Leu Asp Leu Gly Glu Thr Lys Gly
280 285 290
act gac gtt aag gat cct gag aag gtt ctg acc cgt aag cct tcc gac 1027
Thr Asp Val Lys Asp Pro Glu Lys Val Leu Thr Arg Lys Pro Ser Asp
295 300 305
gaa gag cca ctg tct gca ctt gca ttc aag att gca gct cac cca ttc 1075
Glu Glu Pro Leu Ser Ala Leu Ala Phe Lys Ile Ala Ala His Pro Phe
310 315 320 325
ttc ggt aag ctg acc ttc gtt cgt ctg tac tcc ggc aag gtt gag cca 1123
Phe Gly Lys Leu Thr Phe Val Arg Leu Tyr Ser Gly Lys Val Glu Pro
330 335 340
ggc gag cag gtt ctt aac tcc acc aag aac aag aag gaa cgc att ggt 1171
Gly Glu Gln Val Leu Asn Ser Thr Lys Asn Lys Lys Glu Arg Ile Gly
345 350 355
aag ctg ttc cag atg cac gcc aac aag gaa aac cct gtt gag gtt gca 1219
Lys Leu Phe Gln Met His Ala Asn Lys Glu Asn Pro Val Glu Val Ala
360 365 370
cac gct ggt aac atc tac gcg ttc atc ggc ctg aag gac acc acc acc 1267
His Ala Gly Asn Ile Tyr Ala Phe Ile Gly Leu Lys Asp Thr Thr Thr
375 380 385
ggt gac acc ctc tgt gac gca aac gct cca atc att ctt gag tcc atg 1315
Gly Asp Thr Leu Cys Asp Ala Asn Ala Pro Ile Ile Leu Glu Ser Met
390 395 400 405
gac ttc ccg gat cca gtt atc cag gtt gct att gag cct aag acc aag 1363
Asp Phe Pro Asp Pro Val Ile Gln Val Ala Ile Glu Pro Lys Thr Lys
410 415 420
tct gac cag gag aag ctc ggc gta gct atc cag aag ctt gct gaa gaa 1411
Ser Asp Gln Glu Lys Leu Gly Val Ala Ile Gln Lys Leu Ala Glu Glu
425 430 435
gac cca acc ttc acc gtt cac ttg gac gat gag tcc ggc cag acc gtc 1459
Asp Pro Thr Phe Thr Val His Leu Asp Asp Glu Ser Gly Gln Thr Val
440 445 450
att ggc ggc atg ggc gag ctg cac ctc gat gtt ctt gtt gac cgc atg 1507
Ile Gly Gly Met Gly Glu Leu His Leu Asp Val Leu Val Asp Arg Met
455 460 465
aag cgc gag ttc aag gtt gag gca aac atc ggt gac cca cag gtt gct 1555
Lys Arg Glu Phe Lys Val Glu Ala Asn Ile Gly Asp Pro Gln Val Ala
470 475 480 485
tac cgt gag acc atc cgt aag cct gtt gag tcc ctc agc tac acc cac 1603
Tyr Arg Glu Thr Ile Arg Lys Pro Val Glu Ser Leu Ser Tyr Thr His
490 495 500
aag aag cag act ggt ggt tcc ggt cag ttc gct aag gtc atc atc acc 1651
Lys Lys Gln Thr Gly Gly Ser Gly Gln Phe Ala Lys Val Ile Ile Thr
505 510 515
att gag cct tac gca cct gag gca gac gag ctt gaa gag ggc gag tcc 1699
Ile Glu Pro Tyr Ala Pro Glu Ala Asp Glu Leu Glu Glu Gly Glu Ser
520 525 530
gca atc tac aag ttc gag aac gct gtc acc ggt ggt cgt gtt cca cgt 1747
Ala Ile Tyr Lys Phe Glu Asn Ala Val Thr Gly Gly Arg Val Pro Arg
535 540 545
gaa tac atc cca tcc gtt gac gct ggt atc cag gac gca atg cag tac 1795
Glu Tyr Ile Pro Ser Val Asp Ala Gly Ile Gln Asp Ala Met Gln Tyr
550 555 560 565
ggc ttc ctg gct ggc tac cca ctg gtt aac gtc aag gca acc ctt gaa 1843
Gly Phe Leu Ala Gly Tyr Pro Leu Val Asn Val Lys Ala Thr Leu Glu
570 575 580
gat ggc gct tac cac gac gtt gac tcc tct gaa atg gcc ttc aag ctc 1891
Asp Gly Ala Tyr His Asp Val Asp Ser Ser Glu Met Ala Phe Lys Leu
585 590 595
gcc ggt tcc cag gcg ttc aag gaa gct gtt gca aag gca aag cca gtc 1939
Ala Gly Ser Gln Ala Phe Lys Glu Ala Val Ala Lys Ala Lys Pro Val
600 605 610
ctc ctc gag cca atc atg tcc gtt gaa atc acc act cct gag gag tac 1987
Leu Leu Glu Pro Ile Met Ser Val Glu Ile Thr Thr Pro Glu Glu Tyr
615 620 625
atg ggt gaa gtc atc ggt gac gtg aac tcc cgc cgt ggc cag atc gct 2035
Met Gly Glu Val Ile Gly Asp Val Asn Ser Arg Arg Gly Gln Ile Ala
630 635 640 645
tcc atg gat gac cgt gca ggc gcc aag ctg gtt aag gct aag gtt cca 2083
Ser Met Asp Asp Arg Ala Gly Ala Lys Leu Val Lys Ala Lys Val Pro
650 655 660
ctg tct cag atg ttc ggt tac gtc ggt gac ctt cgc tct aag acc cag 2131
Leu Ser Gln Met Phe Gly Tyr Val Gly Asp Leu Arg Ser Lys Thr Gln
665 670 675
ggt cgt gca aac tac tcc atg gtc ttc gat tcc tac gct gag gtc cca 2179
Gly Arg Ala Asn Tyr Ser Met Val Phe Asp Ser Tyr Ala Glu Val Pro
680 685 690
gcc aac gtt gcc gca gat gtt att gct gag cgc aac ggc acc gct tcc 2227
Ala Asn Val Ala Ala Asp Val Ile Ala Glu Arg Asn Gly Thr Ala Ser
695 700 705
taaagatcgt ttagatccga aggaaaacgt 2257
<210> 24
<211> 709
<212> PRT
<213> Corynebacterium glutamicum
<400> 24
Val Ala Gln Glu Val Leu Lys Asp Leu Asn Lys Val Arg Asn Ile Gly
1 5 10 15
Ile Met Ala His Ile Asp Ala Gly Lys Thr Thr Thr Thr Glu Arg Ile
20 25 30
Leu Phe Tyr Thr Gly Ile Asn Arg Lys Val Gly Glu Thr His Asp Gly
35 40 45
Gly Ala Thr Thr Asp Trp Met Glu Gln Glu Lys Glu Arg Gly Ile Thr
50 55 60
Ile Thr Ser Ala Ala Val Thr Cys Phe Trp Asp Asn Asn Gln Val Asn
65 70 75 80
Ile Ile Asp Thr Pro Gly His Val Asp Phe Thr Val Glu Val Glu Arg
85 90 95
Ser Leu Arg Val Leu Asp Gly Ala Val Ala Val Phe Asp Gly Lys Glu
100 105 110
Gly Val Glu Pro Gln Ser Glu Gln Val Trp Arg Gln Ala Thr Lys Tyr
115 120 125
Asp Val Pro Arg Ile Cys Phe Val Asn Lys Met Asp Lys Leu Gly Ala
130 135 140
Asp Phe Tyr Phe Thr Val Gly Thr Ile Glu Asp Arg Leu Gly Ala Lys
145 150 155 160
Pro Leu Val Met Gln Leu Pro Ile Gly Ala Glu Asp Asn Phe Asp Gly
165 170 175
Val Ile Asp Leu Leu Glu Met Lys Ala Leu Thr Trp Arg Gly Val Thr
180 185 190
Pro Ile Gly Thr Glu Ala Thr Val Glu Glu Ile Pro Ala Glu Leu Ala
195 200 205
Asp Arg Ala Ala Glu Tyr Arg Glu Lys Leu Leu Glu Thr Val Ala Glu
210 215 220
Ser Asp Glu Glu Leu Met Glu Lys Tyr Phe Gly Gly Glu Glu Leu Ser
225 230 235 240
Ile Ala Glu Ile Lys Ala Ala Ile Arg Lys Met Val Val Asn Ser Glu
245 250 255
Ile Tyr Pro Val Tyr Cys Gly Thr Ala Tyr Lys Asn Lys Gly Ile Gln
260 265 270
Pro Leu Leu Asp Ala Val Val Asp Phe Leu Pro Ser Pro Leu Asp Leu
275 280 285
Gly Glu Thr Lys Gly Thr Asp Val Lys Asp Pro Glu Lys Val Leu Thr
290 295 300
Arg Lys Pro Ser Asp Glu Glu Pro Leu Ser Ala Leu Ala Phe Lys Ile
305 310 315 320
Ala Ala His Pro Phe Phe Gly Lys Leu Thr Phe Val Arg Leu Tyr Ser
325 330 335
Gly Lys Val Glu Pro Gly Glu Gln Val Leu Asn Ser Thr Lys Asn Lys
340 345 350
Lys Glu Arg Ile Gly Lys Leu Phe Gln Met His Ala Asn Lys Glu Asn
355 360 365
Pro Val Glu Val Ala His Ala Gly Asn Ile Tyr Ala Phe Ile Gly Leu
370 375 380
Lys Asp Thr Thr Thr Gly Asp Thr Leu Cys Asp Ala Asn Ala Pro Ile
385 390 395 400
Ile Leu Glu Ser Met Asp Phe Pro Asp Pro Val Ile Gln Val Ala Ile
405 410 415
Glu Pro Lys Thr Lys Ser Asp Gln Glu Lys Leu Gly Val Ala Ile Gln
420 425 430
Lys Leu Ala Glu Glu Asp Pro Thr Phe Thr Val His Leu Asp Asp Glu
435 440 445
Ser Gly Gln Thr Val Ile Gly Gly Met Gly Glu Leu His Leu Asp Val
450 455 460
Leu Val Asp Arg Met Lys Arg Glu Phe Lys Val Glu Ala Asn Ile Gly
465 470 475 480
Asp Pro Gln Val Ala Tyr Arg Glu Thr Ile Arg Lys Pro Val Glu Ser
485 490 495
Leu Ser Tyr Thr His Lys Lys Gln Thr Gly Gly Ser Gly Gln Phe Ala
500 505 510
Lys Val Ile Ile Thr Ile Glu Pro Tyr Ala Pro Glu Ala Asp Glu Leu
515 520 525
Glu Glu Gly Glu Ser Ala Ile Tyr Lys Phe Glu Asn Ala Val Thr Gly
530 535 540
Gly Arg Val Pro Arg Glu Tyr Ile Pro Ser Val Asp Ala Gly Ile Gln
545 550 555 560
Asp Ala Met Gln Tyr Gly Phe Leu Ala Gly Tyr Pro Leu Val Asn Val
565 570 575
Lys Ala Thr Leu Glu Asp Gly Ala Tyr His Asp Val Asp Ser Ser Glu
580 585 590
Met Ala Phe Lys Leu Ala Gly Ser Gln Ala Phe Lys Glu Ala Val Ala
595 600 605
Lys Ala Lys Pro Val Leu Leu Glu Pro Ile Met Ser Val Glu Ile Thr
610 615 620
Thr Pro Glu Glu Tyr Met Gly Glu Val Ile Gly Asp Val Asn Ser Arg
625 630 635 640
Arg Gly Gln Ile Ala Ser Met Asp Asp Arg Ala Gly Ala Lys Leu Val
645 650 655
Lys Ala Lys Val Pro Leu Ser Gln Met Phe Gly Tyr Val Gly Asp Leu
660 665 670
Arg Ser Lys Thr Gln Gly Arg Ala Asn Tyr Ser Met Val Phe Asp Ser
675 680 685
Tyr Ala Glu Val Pro Ala Asn Val Ala Ala Asp Val Ile Ala Glu Arg
690 695 700
Asn Gly Thr Ala Ser
705
<210> 25
<211> 1318
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1288)
<223> RXA01284
<400> 25
atctgtgtgc tcagtcttcc aggctgctta tcacagtgaa agcaaaacca attcgtggct 60
gcgaaagtcg tagccaccac gaagtccagg aggacataca gtg gca aag gcg aag 115
Val Ala Lys Ala Lys
1 5
ttc gag cgt acc aag ccc cac gta aac atc ggc acc atc ggt cac gtt 163
Phe Glu Arg Thr Lys Pro His Val Asn Ile Gly Thr Ile Gly His Val
10 15 20
gac cac ggt aag acc acc acc acc gcg gct atc acc aag gtt ctg gct 211
Asp His Gly Lys Thr Thr Thr Thr Ala Ala Ile Thr Lys Val Leu Ala
25 30 35
gac act tac cct gag ctc aac gag gct ttc gcc ttc gac tcc atc gat 259
Asp Thr Tyr Pro Glu Leu Asn Glu Ala Phe Ala Phe Asp Ser Ile Asp
40 45 50
aag gct cct gag gag aag gag cgt ggc atc acg atc aac atc tcc cac 307
Lys Ala Pro Glu Glu Lys Glu Arg Gly Ile Thr Ile Asn Ile Ser His
55 60 65
gtt gag tac cag act gaa aag cgc cac tac gca cac gtt gac gct cca 355
Val Glu Tyr Gln Thr Glu Lys Arg His Tyr Ala His Val Asp Ala Pro
70 75 80 85
ggc cac gcc gac tac atc aag aac atg att acc ggc gct gct cag atg 403
Gly His Ala Asp Tyr Ile Lys Asn Met Ile Thr Gly Ala Ala Gln Met
90 95 100
gac ggc gca atc ctc gtt gtt gct gct acc gac ggc cca atg cct cag 451
Asp Gly Ala Ile Leu Val Val Ala Ala Thr Asp Gly Pro Met Pro Gln
105 110 115
acc cgt gag cac gtt ctt ctt gct cgc cag gtt ggc gtt cct tac atc 499
Thr Arg Glu His Val Leu Leu Ala Arg Gln Val Gly Val Pro Tyr Ile
120 125 130
ctc gtt gct ctt aac aag tgc gac atg gtt gag gat gag gaa atc atc 547
Leu Val Ala Leu Asn Lys Cys Asp Met Val Glu Asp Glu Glu Ile Ile
135 140 145
gag ctc gtc gag atg gaa gtt cgt gaa ctt ctt gct gag cag gac tac 595
Glu Leu Val Glu Met Glu Val Arg Glu Leu Leu Ala Glu Gln Asp Tyr
150 155 160 165
gac gaa gag gct cca att gtt cac atc tcc gct ctg aag gct ctt gag 643
Asp Glu Glu Ala Pro Ile Val His Ile Ser Ala Leu Lys Ala Leu Glu
170 175 180
ggc gac gag aag tgg ggc aag cag atc ctt gag ctc atg cag gct tgc 691
Gly Asp Glu Lys Trp Gly Lys Gln Ile Leu Glu Leu Met Gln Ala Cys
185 190 195
gat gac aac atc cct gac cca gtt cgt gag acc gac aag cca ttc ctc 739
Asp Asp Asn Ile Pro Asp Pro Val Arg Glu Thr Asp Lys Pro Phe Leu
200 205 210
atg cct atc gag gac atc ttc acc atc acc ggt cgt ggc acc gtt gtt 787
Met Pro Ile Glu Asp Ile Phe Thr Ile Thr Gly Arg Gly Thr Val Val
215 220 225
acc ggt cgt gtt gag cgc ggt acc ctg aac gtg aac gat gat gtt gac 835
Thr Gly Arg Val Glu Arg Gly Thr Leu Asn Val Asn Asp Asp Val Asp
230 235 240 245
atc atc ggc atc aag gag aag tcc acc tcc acc acc gtt acc ggt atc 883
Ile Ile Gly Ile Lys Glu Lys Ser Thr Ser Thr Thr Val Thr Gly Ile
250 255 260
gag atg ttc cgt aag ctt ctt gac tcc gct gag gct ggc gac aac tgt 931
Glu Met Phe Arg Lys Leu Leu Asp Ser Ala Glu Ala Gly Asp Asn Cys
265 270 275
ggt ctg ctt ctc cgt ggt atc aag cgc gaa gat gtt gag cgt ggc cag 979
Gly Leu Leu Leu Arg Gly Ile Lys Arg Glu Asp Val Glu Arg Gly Gln
280 285 290
gtt atc gtt aag cca ggc gct tac acc cct cac acc gag ttc gag ggc 1027
Val Ile Val Lys Pro Gly Ala Tyr Thr Pro His Thr Glu Phe Glu Gly
295 300 305
tct gtc tac gtt ctg tcc aag gat gaa ggt ggc cgc cac acc cca ttc 1075
Ser Val Tyr Val Leu Ser Lys Asp Glu Gly Gly Arg His Thr Pro Phe
310 315 320 325
ttc gac aac tac cgt cct cag ttc tac ttc cgc acc acc gac gtt acc 1123
Phe Asp Asn Tyr Arg Pro Gln Phe Tyr Phe Arg Thr Thr Asp Val Thr
330 335 340
ggt gtt gtg aag ctt cca gag ggc acc gag atg gtc atg cct ggc gac 1171
Gly Val Val Lys Leu Pro Glu Gly Thr Glu Met Val Met Pro Gly Asp
345 350 355
aac gtc gac atg tcc gtc acc ctg atc cag cct gtc gct atg gac gag 1219
Asn Val Asp Met Ser Val Thr Leu Ile Gln Pro Val Ala Met Asp Glu
360 365 370
ggc ctg cgt ttc gct atc cgc gaa ggc tcc cgc acc gtt ggc gct ggt 1267
Gly Leu Arg Phe Ala Ile Arg Glu Gly Ser Arg Thr Val Gly Ala Gly
375 380 385
cgt gtc acc aag atc atc aag taatttgatg ctctaactgt tgaggtcttt 1318
Arg Val Thr Lys Ile Ile Lys
390 395
<210> 26
<211> 396
<212> PRT
<213> Corynebacterium glutamicum
<400> 26
Val Ala Lys Ala Lys Phe Glu Arg Thr Lys Pro His Val Asn Ile Gly
1 5 10 15
Thr Ile Gly His Val Asp His Gly Lys Thr Thr Thr Thr Ala Ala Ile
20 25 30
Thr Lys Val Leu Ala Asp Thr Tyr Pro Glu Leu Asn Glu Ala Phe Ala
35 40 45
Phe Asp Ser Ile Asp Lys Ala Pro Glu Glu Lys Glu Arg Gly Ile Thr
50 55 60
Ile Asn Ile Ser His Val Glu Tyr Gln Thr Glu Lys Arg His Tyr Ala
65 70 75 80
His Val Asp Ala Pro Gly His Ala Asp Tyr Ile Lys Asn Met Ile Thr
85 90 95
Gly Ala Ala Gln Met Asp Gly Ala Ile Leu Val Val Ala Ala Thr Asp
100 105 110
Gly Pro Met Pro Gln Thr Arg Glu His Val Leu Leu Ala Arg Gln Val
115 120 125
Gly Val Pro Tyr Ile Leu Val Ala Leu Asn Lys Cys Asp Met Val Glu
130 135 140
Asp Glu Glu Ile Ile Glu Leu Val Glu Met Glu Val Arg Glu Leu Leu
145 150 155 160
Ala Glu Gln Asp Tyr Asp Glu Glu Ala Pro Ile Val His Ile Ser Ala
165 170 175
Leu Lys Ala Leu Glu Gly Asp Glu Lys Trp Gly Lys Gln Ile Leu Glu
180 185 190
Leu Met Gln Ala Cys Asp Asp Asn Ile Pro Asp Pro Val Arg Glu Thr
195 200 205
Asp Lys Pro Phe Leu Met Pro Ile Glu Asp Ile Phe Thr Ile Thr Gly
210 215 220
Arg Gly Thr Val Val Thr Gly Arg Val Glu Arg Gly Thr Leu Asn Val
225 230 235 240
Asn Asp Asp Val Asp Ile Ile Gly Ile Lys Glu Lys Ser Thr Ser Thr
245 250 255
Thr Val Thr Gly Ile Glu Met Phe Arg Lys Leu Leu Asp Ser Ala Glu
260 265 270
Ala Gly Asp Asn Cys Gly Leu Leu Leu Arg Gly Ile Lys Arg Glu Asp
275 280 285
Val Glu Arg Gly Gln Val Ile Val Lys Pro Gly Ala Tyr Thr Pro His
290 295 300
Thr Glu Phe Glu Gly Ser Val Tyr Val Leu Ser Lys Asp Glu Gly Gly
305 310 315 320
Arg His Thr Pro Phe Phe Asp Asn Tyr Arg Pro Gln Phe Tyr Phe Arg
325 330 335
Thr Thr Asp Val Thr Gly Val Val Lys Leu Pro Glu Gly Thr Glu Met
340 345 350
Val Met Pro Gly Asp Asn Val Asp Met Ser Val Thr Leu Ile Gln Pro
355 360 365
Val Ala Met Asp Glu Gly Leu Arg Phe Ala Ile Arg Glu Gly Ser Arg
370 375 380
Thr Val Gly Ala Gly Arg Val Thr Lys Ile Ile Lys
385 390 395
<210> 27
<211> 3625
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(3595)
<223> RXA01344
<400> 27
gggggatcgg gttcctcagc agaccaattg ctcaaaaata ccagcggtgt tgatctgcac 60
ttaatggcct tgaccagcca ggtgcaatta cccgcgtgag gtg ctg gaa gga ccc 115
Val Leu Glu Gly Pro
1 5
atc ttg gca gtc tcc cgc cag acc aag tca gtc gtc gat att ccc ggt 163
Ile Leu Ala Val Ser Arg Gln Thr Lys Ser Val Val Asp Ile Pro Gly
10 15 20
gca ccg cag cgt tat tct ttc gcg aag gtg tcc gca ccc att gag gtg 211
Ala Pro Gln Arg Tyr Ser Phe Ala Lys Val Ser Ala Pro Ile Glu Val
25 30 35
ccc ggg cta cta gat ctt caa ctg gat tct tac tcc tgg ctg att ggt 259
Pro Gly Leu Leu Asp Leu Gln Leu Asp Ser Tyr Ser Trp Leu Ile Gly
40 45 50
acg cct gag tgg cgt gct cgt cag aag gaa gaa ttc ggc gag gga gcc 307
Thr Pro Glu Trp Arg Ala Arg Gln Lys Glu Glu Phe Gly Glu Gly Ala
55 60 65
cgc gta acc agc ggc ctt gag aac att ctc gag gag ctc tcc cca atc 355
Arg Val Thr Ser Gly Leu Glu Asn Ile Leu Glu Glu Leu Ser Pro Ile
70 75 80 85
cag gat tac tct gga aac atg tcc ctg agc ctt tcg gag cca cgc ttc 403
Gln Asp Tyr Ser Gly Asn Met Ser Leu Ser Leu Ser Glu Pro Arg Phe
90 95 100
gaa gac gtc aag aac acc att gac gag gcg aaa gaa aag gac atc aac 451
Glu Asp Val Lys Asn Thr Ile Asp Glu Ala Lys Glu Lys Asp Ile Asn
105 110 115
tac gcg gcg cca ctg tat gtg acc gcg gag ttc gtc aac aac acc acc 499
Tyr Ala Ala Pro Leu Tyr Val Thr Ala Glu Phe Val Asn Asn Thr Thr
120 125 130
ggt gaa atc aag tct cag act gtc ttc atc ggc gat ttc cca atg atg 547
Gly Glu Ile Lys Ser Gln Thr Val Phe Ile Gly Asp Phe Pro Met Met
135 140 145
acg gac aag gga acg ttc atc atc aac gga acc gaa cgc gtt gtg gtc 595
Thr Asp Lys Gly Thr Phe Ile Ile Asn Gly Thr Glu Arg Val Val Val
150 155 160 165
agc cag ctc gtc cgc tcc ccg ggc gtg tac ttt gac cag acc atc gat 643
Ser Gln Leu Val Arg Ser Pro Gly Val Tyr Phe Asp Gln Thr Ile Asp
170 175 180
aag tca act gag cgt cca ctg cac gcc gtg aag gtt att cct tcc cgt 691
Lys Ser Thr Glu Arg Pro Leu His Ala Val Lys Val Ile Pro Ser Arg
185 190 195
ggt gct tgg ctt gag ttt gac gtc gat aag cgc gat tcg gtt ggt gtt 739
Gly Ala Trp Leu Glu Phe Asp Val Asp Lys Arg Asp Ser Val Gly Val
200 205 210
cgt att gac cgc aag cgt cgc cag cca gtc acc gta ctg ctg aag gct 787
Arg Ile Asp Arg Lys Arg Arg Gln Pro Val Thr Val Leu Leu Lys Ala
215 220 225
ctt ggc tgg acc act gag cag atc acc gag cgt ttc ggt ttc tct gaa 835
Leu Gly Trp Thr Thr Glu Gln Ile Thr Glu Arg Phe Gly Phe Ser Glu
230 235 240 245
atc atg atg tcc acc ctc gag tcc gat ggt gta gca aac acc gat gag 883
Ile Met Met Ser Thr Leu Glu Ser Asp Gly Val Ala Asn Thr Asp Glu
250 255 260
gca ttg ctg gag atc tac cgc aag cag cgt cca ggc gag cag cct acc 931
Ala Leu Leu Glu Ile Tyr Arg Lys Gln Arg Pro Gly Glu Gln Pro Thr
265 270 275
cgc gac ctt gcg cag tcc ctc ctg gac aac agc ttc ttc cgt gca aag 979
Arg Asp Leu Ala Gln Ser Leu Leu Asp Asn Ser Phe Phe Arg Ala Lys
280 285 290
cgc tac gac ctg gct cgc gtt ggt cgt tac aag atc aac cgc aag ctc 1027
Arg Tyr Asp Leu Ala Arg Val Gly Arg Tyr Lys Ile Asn Arg Lys Leu
295 300 305
ggc ctt ggt ggc gac cac gat ggt ttg atg act ctt act gaa gag gac 1075
Gly Leu Gly Gly Asp His Asp Gly Leu Met Thr Leu Thr Glu Glu Asp
310 315 320 325
atc gca acc acc atc gag tac ctg gtg cgt ctg cac gca ggt gag cgc 1123
Ile Ala Thr Thr Ile Glu Tyr Leu Val Arg Leu His Ala Gly Glu Arg
330 335 340
gtc atg act tct cca aat ggt gaa gag atc cca gtc gag acc gat gac 1171
Val Met Thr Ser Pro Asn Gly Glu Glu Ile Pro Val Glu Thr Asp Asp
345 350 355
atc gac cac ttt ggt aac cgt cgt ctg cgt acc gtt ggc gaa ctg atc 1219
Ile Asp His Phe Gly Asn Arg Arg Leu Arg Thr Val Gly Glu Leu Ile
360 365 370
cag aac cag gtc cgt gtc ggc ctg tcc cgc atg gag cgc gtt gtt cgt 1267
Gln Asn Gln Val Arg Val Gly Leu Ser Arg Met Glu Arg Val Val Arg
375 380 385
gag cgt atg acc acc cag gat gcg gag tcc att act cct act tcc ttg 1315
Glu Arg Met Thr Thr Gln Asp Ala Glu Ser Ile Thr Pro Thr Ser Leu
390 395 400 405
atc aac gtt cgt cct gtc tct gca gct atc cgt gag ttc ttc gga act 1363
Ile Asn Val Arg Pro Val Ser Ala Ala Ile Arg Glu Phe Phe Gly Thr
410 415 420
tcc cag ctg tct cag ttc atg gac cag aac aac tcc ctg tct ggt ttg 1411
Ser Gln Leu Ser Gln Phe Met Asp Gln Asn Asn Ser Leu Ser Gly Leu
425 430 435
act cac aag cgt cgt ctg tcg gct ctg ggc ccg ggt ggt ctg tcc cgt 1459
Thr His Lys Arg Arg Leu Ser Ala Leu Gly Pro Gly Gly Leu Ser Arg
440 445 450
gag cgc gcc ggc atc gag gtt cga gac gtt cac cca tct cac tac ggc 1507
Glu Arg Ala Gly Ile Glu Val Arg Asp Val His Pro Ser His Tyr Gly
455 460 465
cgt atg tgc cca att gag act ccg gaa ggt cca aac att ggc ctg atc 1555
Arg Met Cys Pro Ile Glu Thr Pro Glu Gly Pro Asn Ile Gly Leu Ile
470 475 480 485
ggt tcc ttg gct tcc tat gct cga gtg aac cca ttc ggt ttc att gag 1603
Gly Ser Leu Ala Ser Tyr Ala Arg Val Asn Pro Phe Gly Phe Ile Glu
490 495 500
acc cca tac cgt cgc atc atc gac ggc aag ctg acc gac cag att gac 1651
Thr Pro Tyr Arg Arg Ile Ile Asp Gly Lys Leu Thr Asp Gln Ile Asp
505 510 515
tac ctt acc gct gat gag gaa gac cgc ttc gtt gtt gcg cag gca aac 1699
Tyr Leu Thr Ala Asp Glu Glu Asp Arg Phe Val Val Ala Gln Ala Asn
520 525 530
acg cac tac gac gaa gag ggc aac atc acc gat gag acc gtc act gtt 1747
Thr His Tyr Asp Glu Glu Gly Asn Ile Thr Asp Glu Thr Val Thr Val
535 540 545
cgt ctg aag gac ggc gac atc gcc atg gtt ggc cgc aac gcg gtt gat 1795
Arg Leu Lys Asp Gly Asp Ile Ala Met Val Gly Arg Asn Ala Val Asp
550 555 560 565
tac atg gac gtt tcc cct cgt cag atg gtt tct gtt ggt acc gcg atg 1843
Tyr Met Asp Val Ser Pro Arg Gln Met Val Ser Val Gly Thr Ala Met
570 575 580
att cca ttc ctg gag cac gac gat gct aac cgt gca ctg atg ggc gcg 1891
Ile Pro Phe Leu Glu His Asp Asp Ala Asn Arg Ala Leu Met Gly Ala
585 590 595
aac atg cag aag cag gct gtg cca ctg att cgt gcc gag gct cct ttc 1939
Asn Met Gln Lys Gln Ala Val Pro Leu Ile Arg Ala Glu Ala Pro Phe
600 605 610
gtg ggc acc ggt atg gag cag cgc gca gca tac gac gcc ggc gac ctg 1987
Val Gly Thr Gly Met Glu Gln Arg Ala Ala Tyr Asp Ala Gly Asp Leu
615 620 625
gtt att acc cca gtc gca ggt gtg gtg gaa aac gtt tca gct gac ttc 2035
Val Ile Thr Pro Val Ala Gly Val Val Glu Asn Val Ser Ala Asp Phe
630 635 640 645
atc acc atc atg gct gat gac ggc aag cgc gaa acc tac ctg ctg cgt 2083
Ile Thr Ile Met Ala Asp Asp Gly Lys Arg Glu Thr Tyr Leu Leu Arg
650 655 660
aag ttc cag cgc acc aac cag ggc acc agc tac aac cag aag cct ttg 2131
Lys Phe Gln Arg Thr Asn Gln Gly Thr Ser Tyr Asn Gln Lys Pro Leu
665 670 675
gtt aac ttg ggc gag cgc gtt gaa gct ggc cag gtt att gct gat ggt 2179
Val Asn Leu Gly Glu Arg Val Glu Ala Gly Gln Val Ile Ala Asp Gly
680 685 690
cca ggt acc ttc aat ggt gaa atg tcc ctt ggc cgt aac ctt ctg gtt 2227
Pro Gly Thr Phe Asn Gly Glu Met Ser Leu Gly Arg Asn Leu Leu Val
695 700 705
gcg ttc atg cct tgg gaa ggc cac aac tac gag gat gcg atc atc ctc 2275
Ala Phe Met Pro Trp Glu Gly His Asn Tyr Glu Asp Ala Ile Ile Leu
710 715 720 725
aac cag aac atc gtt gag cag gac atc ttg acc tcg atc cac atc gag 2323
Asn Gln Asn Ile Val Glu Gln Asp Ile Leu Thr Ser Ile His Ile Glu
730 735 740
gag cac gag atc gat gcc cgc gac act aag ctt ggc gcc gaa gaa atc 2371
Glu His Glu Ile Asp Ala Arg Asp Thr Lys Leu Gly Ala Glu Glu Ile
745 750 755
acc cgc gac atc cct aat gtg tct gaa gaa gtc ctc aag gac ctc gac 2419
Thr Arg Asp Ile Pro Asn Val Ser Glu Glu Val Leu Lys Asp Leu Asp
760 765 770
gac cgc ggt att gtc cgc atc ggt gct gat gtt cgt gac ggc gac atc 2467
Asp Arg Gly Ile Val Arg Ile Gly Ala Asp Val Arg Asp Gly Asp Ile
775 780 785
ctg gtc ggt aag gtc acc cct aag ggc gag acc gag ctc acc ccg gaa 2515
Leu Val Gly Lys Val Thr Pro Lys Gly Glu Thr Glu Leu Thr Pro Glu
790 795 800 805
gag cgc ttg ctg cgc gca atc ttc ggt gag aag gcc cgc gaa gtt cgc 2563
Glu Arg Leu Leu Arg Ala Ile Phe Gly Glu Lys Ala Arg Glu Val Arg
810 815 820
gat acc tcc atg aag gtg cct cac ggt gag acc ggc aag gtc atc ggc 2611
Asp Thr Ser Met Lys Val Pro His Gly Glu Thr Gly Lys Val Ile Gly
825 830 835
gtg cgt cac ttc tcc cgc gag gac gac gac gat ctg gct cct ggc gtc 2659
Val Arg His Phe Ser Arg Glu Asp Asp Asp Asp Leu Ala Pro Gly Val
840 845 850
aac gag atg atc cgt atc tac gtt gct cag aag cgt aag atc cag gac 2707
Asn Glu Met Ile Arg Ile Tyr Val Ala Gln Lys Arg Lys Ile Gln Asp
855 860 865
ggc gat aag ctc gct ggc cgc cac ggt aac aag ggt gtt gtc ggt aaa 2755
Gly Asp Lys Leu Ala Gly Arg His Gly Asn Lys Gly Val Val Gly Lys
870 875 880 885
att ttg cct cag gaa gat atg cca ttc ctt cca gac ggc act cct gtt 2803
Ile Leu Pro Gln Glu Asp Met Pro Phe Leu Pro Asp Gly Thr Pro Val
890 895 900
gac atc atc ttg aac acc cac ggt gtt cca cgt cgt atg aac att ggt 2851
Asp Ile Ile Leu Asn Thr His Gly Val Pro Arg Arg Met Asn Ile Gly
905 910 915
cag gtt ctt gag acc cac ctt ggc tgg ctg gca tct gct ggt tgg tcc 2899
Gln Val Leu Glu Thr His Leu Gly Trp Leu Ala Ser Ala Gly Trp Ser
920 925 930
gtg gat cct gaa gat cct gag aac gct gag ctc gtc aag act ctg cct 2947
Val Asp Pro Glu Asp Pro Glu Asn Ala Glu Leu Val Lys Thr Leu Pro
935 940 945
gca gac ctc ctc gag gtt cct gct ggt tcc ttg act gca act cct gtg 2995
Ala Asp Leu Leu Glu Val Pro Ala Gly Ser Leu Thr Ala Thr Pro Val
950 955 960 965
ttc gac ggt gcg tca aac gaa gag ctc gca ggc ctg ctc gct aat tca 3043
Phe Asp Gly Ala Ser Asn Glu Glu Leu Ala Gly Leu Leu Ala Asn Ser
970 975 980
cgt cca aac cgc gac ggc gac gtc atg gtt aac gcg gat ggt aaa gca 3091
Arg Pro Asn Arg Asp Gly Asp Val Met Val Asn Ala Asp Gly Lys Ala
985 990 995
acg ctt atc gac ggt cgc tcc ggt gag cct tac ccg tac ccg gtt tcc 3139
Thr Leu Ile Asp Gly Arg Ser Gly Glu Pro Tyr Pro Tyr Pro Val Ser
1000 1005 1010
atc ggc tac atg tac atg ctg aag ctg cac cac ctc gtt gac gag aag 3187
Ile Gly Tyr Met Tyr Met Leu Lys Leu His His Leu Val Asp Glu Lys
1015 1020 1025
atc cac gca cgt tcc act ggt cct tac tcc atg att acc cag cag cca 3235
Ile His Ala Arg Ser Thr Gly Pro Tyr Ser Met Ile Thr Gln Gln Pro
1030 1035 1040 1045
ctg ggt ggt aaa gca cag ttc ggt gga cag cgt ttc ggc gaa atg gag 3283
Leu Gly Gly Lys Ala Gln Phe Gly Gly Gln Arg Phe Gly Glu Met Glu
1050 1055 1060
gtg tgg gca atg cag gca tac ggc gct gcc tac aca ctt cag gag ctg 3331
Val Trp Ala Met Gln Ala Tyr Gly Ala Ala Tyr Thr Leu Gln Glu Leu
1065 1070 1075
ctg acc atc aag tct gat gac gtg gtt ggc cgt gtc aag gtc tac gaa 3379
Leu Thr Ile Lys Ser Asp Asp Val Val Gly Arg Val Lys Val Tyr Glu
1080 1085 1090
gca att gtg aag ggc gag aac atc ccg gat cca ggt att cct gag tcc 3427
Ala Ile Val Lys Gly Glu Asn Ile Pro Asp Pro Gly Ile Pro Glu Ser
1095 1100 1105
ttc aag gtt ctc ctc aag gag ctc cag tcc ttg tgc ctg aac gtg gag 3475
Phe Lys Val Leu Leu Lys Glu Leu Gln Ser Leu Cys Leu Asn Val Glu
1110 1115 1120 1125
gtt ctc tcc gca gac ggc act cca atg gag ctc gcg ggt gac gac gac 3523
Val Leu Ser Ala Asp Gly Thr Pro Met Glu Leu Ala Gly Asp Asp Asp
1130 1135 1140
gac ttc gat cag gca ggc gcc tca ctt ggc atc aac ctg tcc cgt gac 3571
Asp Phe Asp Gln Ala Gly Ala Ser Leu Gly Ile Asn Leu Ser Arg Asp
1145 1150 1155
gag cgt tcc gac gcc gac acc gca tagcagatca gaaaacaacc gctagaaatc 3625
Glu Arg Ser Asp Ala Asp Thr Ala
1160 1165
<210> 28
<211> 1165
<212> PRT
<213> Corynebacterium glutamicum
<400> 28
Val Leu Glu Gly Pro Ile Leu Ala Val Ser Arg Gln Thr Lys Ser Val
1 5 10 15
Val Asp Ile Pro Gly Ala Pro Gln Arg Tyr Ser Phe Ala Lys Val Ser
20 25 30
Ala Pro Ile Glu Val Pro Gly Leu Leu Asp Leu Gln Leu Asp Ser Tyr
35 40 45
Ser Trp Leu Ile Gly Thr Pro Glu Trp Arg Ala Arg Gln Lys Glu Glu
50 55 60
Phe Gly Glu Gly Ala Arg Val Thr Ser Gly Leu Glu Asn Ile Leu Glu
65 70 75 80
Glu Leu Ser Pro Ile Gln Asp Tyr Ser Gly Asn Met Ser Leu Ser Leu
85 90 95
Ser Glu Pro Arg Phe Glu Asp Val Lys Asn Thr Ile Asp Glu Ala Lys
100 105 110
Glu Lys Asp Ile Asn Tyr Ala Ala Pro Leu Tyr Val Thr Ala Glu Phe
115 120 125
Val Asn Asn Thr Thr Gly Glu Ile Lys Ser Gln Thr Val Phe Ile Gly
130 135 140
Asp Phe Pro Met Met Thr Asp Lys Gly Thr Phe Ile Ile Asn Gly Thr
145 150 155 160
Glu Arg Val Val Val Ser Gln Leu Val Arg Ser Pro Gly Val Tyr Phe
165 170 175
Asp Gln Thr Ile Asp Lys Ser Thr Glu Arg Pro Leu His Ala Val Lys
180 185 190
Val Ile Pro Ser Arg Gly Ala Trp Leu Glu Phe Asp Val Asp Lys Arg
195 200 205
Asp Ser Val Gly Val Arg Ile Asp Arg Lys Arg Arg Gln Pro Val Thr
210 215 220
Val Leu Leu Lys Ala Leu Gly Trp Thr Thr Glu Gln Ile Thr Glu Arg
225 230 235 240
Phe Gly Phe Ser Glu Ile Met Met Ser Thr Leu Glu Ser Asp Gly Val
245 250 255
Ala Asn Thr Asp Glu Ala Leu Leu Glu Ile Tyr Arg Lys Gln Arg Pro
260 265 270
Gly Glu Gln Pro Thr Arg Asp Leu Ala Gln Ser Leu Leu Asp Asn Ser
275 280 285
Phe Phe Arg Ala Lys Arg Tyr Asp Leu Ala Arg Val Gly Arg Tyr Lys
290 295 300
Ile Asn Arg Lys Leu Gly Leu Gly Gly Asp His Asp Gly Leu Met Thr
305 310 315 320
Leu Thr Glu Glu Asp Ile Ala Thr Thr Ile Glu Tyr Leu Val Arg Leu
325 330 335
His Ala Gly Glu Arg Val Met Thr Ser Pro Asn Gly Glu Glu Ile Pro
340 345 350
Val Glu Thr Asp Asp Ile Asp His Phe Gly Asn Arg Arg Leu Arg Thr
355 360 365
Val Gly Glu Leu Ile Gln Asn Gln Val Arg Val Gly Leu Ser Arg Met
370 375 380
Glu Arg Val Val Arg Glu Arg Met Thr Thr Gln Asp Ala Glu Ser Ile
385 390 395 400
Thr Pro Thr Ser Leu Ile Asn Val Arg Pro Val Ser Ala Ala Ile Arg
405 410 415
Glu Phe Phe Gly Thr Ser Gln Leu Ser Gln Phe Met Asp Gln Asn Asn
420 425 430
Ser Leu Ser Gly Leu Thr His Lys Arg Arg Leu Ser Ala Leu Gly Pro
435 440 445
Gly Gly Leu Ser Arg Glu Arg Ala Gly Ile Glu Val Arg Asp Val His
450 455 460
Pro Ser His Tyr Gly Arg Met Cys Pro Ile Glu Thr Pro Glu Gly Pro
465 470 475 480
Asn Ile Gly Leu Ile Gly Ser Leu Ala Ser Tyr Ala Arg Val Asn Pro
485 490 495
Phe Gly Phe Ile Glu Thr Pro Tyr Arg Arg Ile Ile Asp Gly Lys Leu
500 505 510
Thr Asp Gln Ile Asp Tyr Leu Thr Ala Asp Glu Glu Asp Arg Phe Val
515 520 525
Val Ala Gln Ala Asn Thr His Tyr Asp Glu Glu Gly Asn Ile Thr Asp
530 535 540
Glu Thr Val Thr Val Arg Leu Lys Asp Gly Asp Ile Ala Met Val Gly
545 550 555 560
Arg Asn Ala Val Asp Tyr Met Asp Val Ser Pro Arg Gln Met Val Ser
565 570 575
Val Gly Thr Ala Met Ile Pro Phe Leu Glu His Asp Asp Ala Asn Arg
580 585 590
Ala Leu Met Gly Ala Asn Met Gln Lys Gln Ala Val Pro Leu Ile Arg
595 600 605
Ala Glu Ala Pro Phe Val Gly Thr Gly Met Glu Gln Arg Ala Ala Tyr
610 615 620
Asp Ala Gly Asp Leu Val Ile Thr Pro Val Ala Gly Val Val Glu Asn
625 630 635 640
Val Ser Ala Asp Phe Ile Thr Ile Met Ala Asp Asp Gly Lys Arg Glu
645 650 655
Thr Tyr Leu Leu Arg Lys Phe Gln Arg Thr Asn Gln Gly Thr Ser Tyr
660 665 670
Asn Gln Lys Pro Leu Val Asn Leu Gly Glu Arg Val Glu Ala Gly Gln
675 680 685
Val Ile Ala Asp Gly Pro Gly Thr Phe Asn Gly Glu Met Ser Leu Gly
690 695 700
Arg Asn Leu Leu Val Ala Phe Met Pro Trp Glu Gly His Asn Tyr Glu
705 710 715 720
Asp Ala Ile Ile Leu Asn Gln Asn Ile Val Glu Gln Asp Ile Leu Thr
725 730 735
Ser Ile His Ile Glu Glu His Glu Ile Asp Ala Arg Asp Thr Lys Leu
740 745 750
Gly Ala Glu Glu Ile Thr Arg Asp Ile Pro Asn Val Ser Glu Glu Val
755 760 765
Leu Lys Asp Leu Asp Asp Arg Gly Ile Val Arg Ile Gly Ala Asp Val
770 775 780
Arg Asp Gly Asp Ile Leu Val Gly Lys Val Thr Pro Lys Gly Glu Thr
785 790 795 800
Glu Leu Thr Pro Glu Glu Arg Leu Leu Arg Ala Ile Phe Gly Glu Lys
805 810 815
Ala Arg Glu Val Arg Asp Thr Ser Met Lys Val Pro His Gly Glu Thr
820 825 830
Gly Lys Val Ile Gly Val Arg His Phe Ser Arg Glu Asp Asp Asp Asp
835 840 845
Leu Ala Pro Gly Val Asn Glu Met Ile Arg Ile Tyr Val Ala Gln Lys
850 855 860
Arg Lys Ile Gln Asp Gly Asp Lys Leu Ala Gly Arg His Gly Asn Lys
865 870 875 880
Gly Val Val Gly Lys Ile Leu Pro Gln Glu Asp Met Pro Phe Leu Pro
885 890 895
Asp Gly Thr Pro Val Asp Ile Ile Leu Asn Thr His Gly Val Pro Arg
900 905 910
Arg Met Asn Ile Gly Gln Val Leu Glu Thr His Leu Gly Trp Leu Ala
915 920 925
Ser Ala Gly Trp Ser Val Asp Pro Glu Asp Pro Glu Asn Ala Glu Leu
930 935 940
Val Lys Thr Leu Pro Ala Asp Leu Leu Glu Val Pro Ala Gly Ser Leu
945 950 955 960
Thr Ala Thr Pro Val Phe Asp Gly Ala Ser Asn Glu Glu Leu Ala Gly
965 970 975
Leu Leu Ala Asn Ser Arg Pro Asn Arg Asp Gly Asp Val Met Val Asn
980 985 990
Ala Asp Gly Lys Ala Thr Leu Ile Asp Gly Arg Ser Gly Glu Pro Tyr
995 1000 1005
Pro Tyr Pro Val Ser Ile Gly Tyr Met Tyr Met Leu Lys Leu His His
1010 1015 1020
Leu Val Asp Glu Lys Ile His Ala Arg Ser Thr Gly Pro Tyr Ser Met
1025 1030 1035 1040
Ile Thr Gln Gln Pro Leu Gly Gly Lys Ala Gln Phe Gly Gly Gln Arg
1045 1050 1055
Phe Gly Glu Met Glu Val Trp Ala Met Gln Ala Tyr Gly Ala Ala Tyr
1060 1065 1070
Thr Leu Gln Glu Leu Leu Thr Ile Lys Ser Asp Asp Val Val Gly Arg
1075 1080 1085
Val Lys Val Tyr Glu Ala Ile Val Lys Gly Glu Asn Ile Pro Asp Pro
1090 1095 1100
Gly Ile Pro Glu Ser Phe Lys Val Leu Leu Lys Glu Leu Gln Ser Leu
1105 1110 1115 1120
Cys Leu Asn Val Glu Val Leu Ser Ala Asp Gly Thr Pro Met Glu Leu
1125 1130 1135
Ala Gly Asp Asp Asp Asp Phe Asp Gln Ala Gly Ala Ser Leu Gly Ile
1140 1145 1150
Asn Leu Ser Arg Asp Glu Arg Ser Asp Ala Asp Thr Ala
1155 1160 1165
<210> 29
<211> 1582
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1552)
<223> RXA01345
<400> 29
cataacctca ttgaacatgc aaaactaatg cttttggggg gtatgcataa attcgtttcg 60
ttccactgca cagcccgaaa atgctgctag ggtcaagttc atg cgt ttt gga ctt 115
Met Arg Phe Gly Leu
1 5
gac ttg gga act acc cgc aca atc gcg gcc gcc gtg gac cgc gga aac 163
Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala Val Asp Arg Gly Asn
10 15 20
tat ccc atc gtc act gtg gaa gat tct tta ggc gac acc cac gat ttc 211
Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly Asp Thr His Asp Phe
25 30 35
att cca tct gtg gtg gcc ctc aag gca gat agg att gtc gcg ggt tgg 259
Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg Ile Val Ala Gly Trp
40 45 50
gat gct att gag gtt ggg cag gac cac cct tcc ttc gta cgt tct ttc 307
Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser Phe Val Arg Ser Phe
55 60 65
aaa cgc cta ctc tct gaa ccc aat gtc acg gaa gcc acc ccg gtc tac 355
Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu Ala Thr Pro Val Tyr
70 75 80 85
ttg ggc gat cat gta cac cct ttg ggc gcc gtc ctg gag gct ttt gcg 403
Leu Gly Asp His Val His Pro Leu Gly Ala Val Leu Glu Ala Phe Ala
90 95 100
gaa aac gtg gtc act gcg ctg cgt gca ttt cag acg caa ttg gga gat 451
Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln Thr Gln Leu Gly Asp
105 110 115
acc tcc ccg atc gaa gta gtc att ggt gtg ccc gcc aac tcc cac agc 499
Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro Ala Asn Ser His Ser
120 125 130
gcc cag cga ctg ctc acc atg tcc gcc ttc agc gcc aca ggc atc acc 547
Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser Ala Thr Gly Ile Thr
135 140 145
gtt gtc ggt ttg gtc aat gag ccc agc gcc gca gct ttc gag tac acc 595
Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala Ala Phe Glu Tyr Thr
150 155 160 165
cac cgc cac gcc cgc acc tta aac tcc aag cgc caa gcc atc gtg gtt 643
His Arg His Ala Arg Thr Leu Asn Ser Lys Arg Gln Ala Ile Val Val
170 175 180
tat gat ttg gga ggc gga aca ttc gac tcc tcg ctc atc cgc atc gac 691
Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser Leu Ile Arg Ile Asp
185 190 195
ggc acc cac cac gag gtt gtg tcc tcc att ggc att tca cgc ctt ggt 739
Gly Thr His His Glu Val Val Ser Ser Ile Gly Ile Ser Arg Leu Gly
200 205 210
ggc gat gat ttc gat gaa atc ctc ctc caa tgc gcg ctc aag gcc gca 787
Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys Ala Leu Lys Ala Ala
215 220 225
ggc aga cag cac gat gcg ttt ggc aag cgt gct aaa aac acg ctt ctc 835
Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala Lys Asn Thr Leu Leu
230 235 240 245
gac gaa tcc cgc aac gcg aag gaa gct ctt gtt ccg caa tcc cgt cgc 883
Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val Pro Gln Ser Arg Arg
250 255 260
ttg gtt cta gaa att ggc gac gac gac atc acc gtt cca gtg aac aag 931
Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr Val Pro Val Asn Lys
265 270 275
ttc tac gag gct gcc act ccc ctg gtg gaa aaa tcc ttg tcc atc atg 979
Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys Ser Leu Ser Ile Met
280 285 290
gaa ccc ctc atc ggc gtc gat gat ctt aaa gat tcc gac atc gca ggc 1027
Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp Ser Asp Ile Ala Gly
295 300 305
atc tac ctt gtt ggt gga gga tcc tcg ctc cca ctc gtt tcc agg ttg 1075
Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro Leu Val Ser Arg Leu
310 315 320 325
ctc cgc gag cgt ttc ggc cgc cgt gtc cac cgc tcc cca ttc ccc tca 1123
Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg Ser Pro Phe Pro Ser
330 335 340
ggt tcc act gcg gtg ggt ctg gcc atc gcg gct gac cct tcc tct ggt 1171
Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala Asp Pro Ser Ser Gly
345 350 355
ttc cac cta agg gac cgc gtt gcg cga ggc atc ggt gtg ttc cgt gag 1219
Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile Gly Val Phe Arg Glu
360 365 370
cac gat tct ggt cgt gcc gtg agc ttt gac ccg ctg atc gcc ccg gac 1267
His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro Leu Ile Ala Pro Asp
375 380 385
acc gat tct gcg acc gtg gcg aaa cga tgc tac aag gcg gtg cac aac 1315
Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr Lys Ala Val His Asn
390 395 400 405
att ggt tgg ttc agg ttc gtg gaa tac tcc acc gtg tcc gag gat ggc 1363
Ile Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr Val Ser Glu Asp Gly
410 415 420
agc ccc gga gat att tcc ctg ctc agt gaa atc aag att cct ttt gat 1411
Ser Pro Gly Asp Ile Ser Leu Leu Ser Glu Ile Lys Ile Pro Phe Asp
425 430 435
agc tcc atc acc gat gtg gat gct acc gag att tca cgt ttc gat ggc 1459
Ser Ser Ile Thr Asp Val Asp Ala Thr Glu Ile Ser Arg Phe Asp Gly
440 445 450
cca gaa gta gaa gaa acc atc aca gtc aat gac aac ggc gtg gct tcc 1507
Pro Glu Val Glu Glu Thr Ile Thr Val Asn Asp Asn Gly Val Ala Ser
455 460 465
att tcc atc aag ata ctc ggc ggc gtt acc gtc gag cac aca att 1552
Ile Ser Ile Lys Ile Leu Gly Gly Val Thr Val Glu His Thr Ile
470 475 480
tagttaccat tttggtgctg gtggagtcca 1582
<210> 30
<211> 484
<212> PRT
<213> Corynebacterium glutamicum
<400> 30
Met Arg Phe Gly Leu Asp Leu Gly Thr Thr Arg Thr Ile Ala Ala Ala
1 5 10 15
Val Asp Arg Gly Asn Tyr Pro Ile Val Thr Val Glu Asp Ser Leu Gly
20 25 30
Asp Thr His Asp Phe Ile Pro Ser Val Val Ala Leu Lys Ala Asp Arg
35 40 45
Ile Val Ala Gly Trp Asp Ala Ile Glu Val Gly Gln Asp His Pro Ser
50 55 60
Phe Val Arg Ser Phe Lys Arg Leu Leu Ser Glu Pro Asn Val Thr Glu
65 70 75 80
Ala Thr Pro Val Tyr Leu Gly Asp His Val His Pro Leu Gly Ala Val
85 90 95
Leu Glu Ala Phe Ala Glu Asn Val Val Thr Ala Leu Arg Ala Phe Gln
100 105 110
Thr Gln Leu Gly Asp Thr Ser Pro Ile Glu Val Val Ile Gly Val Pro
115 120 125
Ala Asn Ser His Ser Ala Gln Arg Leu Leu Thr Met Ser Ala Phe Ser
130 135 140
Ala Thr Gly Ile Thr Val Val Gly Leu Val Asn Glu Pro Ser Ala Ala
145 150 155 160
Ala Phe Glu Tyr Thr His Arg His Ala Arg Thr Leu Asn Ser Lys Arg
165 170 175
Gln Ala Ile Val Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ser Ser
180 185 190
Leu Ile Arg Ile Asp Gly Thr His His Glu Val Val Ser Ser Ile Gly
195 200 205
Ile Ser Arg Leu Gly Gly Asp Asp Phe Asp Glu Ile Leu Leu Gln Cys
210 215 220
Ala Leu Lys Ala Ala Gly Arg Gln His Asp Ala Phe Gly Lys Arg Ala
225 230 235 240
Lys Asn Thr Leu Leu Asp Glu Ser Arg Asn Ala Lys Glu Ala Leu Val
245 250 255
Pro Gln Ser Arg Arg Leu Val Leu Glu Ile Gly Asp Asp Asp Ile Thr
260 265 270
Val Pro Val Asn Lys Phe Tyr Glu Ala Ala Thr Pro Leu Val Glu Lys
275 280 285
Ser Leu Ser Ile Met Glu Pro Leu Ile Gly Val Asp Asp Leu Lys Asp
290 295 300
Ser Asp Ile Ala Gly Ile Tyr Leu Val Gly Gly Gly Ser Ser Leu Pro
305 310 315 320
Leu Val Ser Arg Leu Leu Arg Glu Arg Phe Gly Arg Arg Val His Arg
325 330 335
Ser Pro Phe Pro Ser Gly Ser Thr Ala Val Gly Leu Ala Ile Ala Ala
340 345 350
Asp Pro Ser Ser Gly Phe His Leu Arg Asp Arg Val Ala Arg Gly Ile
355 360 365
Gly Val Phe Arg Glu His Asp Ser Gly Arg Ala Val Ser Phe Asp Pro
370 375 380
Leu Ile Ala Pro Asp Thr Asp Ser Ala Thr Val Ala Lys Arg Cys Tyr
385 390 395 400
Lys Ala Val His Asn Ile Gly Trp Phe Arg Phe Val Glu Tyr Ser Thr
405 410 415
Val Ser Glu Asp Gly Ser Pro Gly Asp Ile Ser Leu Leu Ser Glu Ile
420 425 430
Lys Ile Pro Phe Asp Ser Ser Ile Thr Asp Val Asp Ala Thr Glu Ile
435 440 445
Ser Arg Phe Asp Gly Pro Glu Val Glu Glu Thr Ile Thr Val Asn Asp
450 455 460
Asn Gly Val Ala Ser Ile Ser Ile Lys Ile Leu Gly Gly Val Thr Val
465 470 475 480
Glu His Thr Ile
<210> 31
<211> 1123
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1093)
<223> RXA01404
<220>
<221> unsure
<222> 12 .. 12
<223> All occurrences of n indicate any nucleotide
<400> 31
gtggatccgg tnttgtgatc cactacgcaa ttggagcgct ccaacacaag ctatatttgt 60
ttaaatgtcc tgtcaatagt tcaagagaaa atcacagaag atg agc aca tcc cgc 115
Met Ser Thr Ser Arg
1 5
ccc aca att tat gac gtc gcc aaa gcc gca ggc gtc tcc aaa tcc ttg 163
Pro Thr Ile Tyr Asp Val Ala Lys Ala Ala Gly Val Ser Lys Ser Leu
10 15 20
gtt tct ctc gtg ctt cgc ggc tcc ccc aac gtg agc aaa gaa tcc gaa 211
Val Ser Leu Val Leu Arg Gly Ser Pro Asn Val Ser Lys Glu Ser Glu
25 30 35
gcc gcg gtc aag acc gcg ata aaa aag ctc aac tac cag cca aat cgc 259
Ala Ala Val Lys Thr Ala Ile Lys Lys Leu Asn Tyr Gln Pro Asn Arg
40 45 50
gcc gca tca gac ctt gcg gcc aag cgc acg cag ctc att gca gtg ctt 307
Ala Ala Ser Asp Leu Ala Ala Lys Arg Thr Gln Leu Ile Ala Val Leu
55 60 65
atc gac gac tac tcc aac ccg tgg ttc atc gac ctg att caa agc ctc 355
Ile Asp Asp Tyr Ser Asn Pro Trp Phe Ile Asp Leu Ile Gln Ser Leu
70 75 80 85
agc gat gtg ctc acc ccc aag ggg tac cga ctg tcc gtc att gac tca 403
Ser Asp Val Leu Thr Pro Lys Gly Tyr Arg Leu Ser Val Ile Asp Ser
90 95 100
tta acc tct caa gcc ggc acc gat ccc att acc agt gca cta tca atg 451
Leu Thr Ser Gln Ala Gly Thr Asp Pro Ile Thr Ser Ala Leu Ser Met
105 110 115
cgc ccc gat gga atc atc atc gcc caa gac atc ccc gat ttc act gtc 499
Arg Pro Asp Gly Ile Ile Ile Ala Gln Asp Ile Pro Asp Phe Thr Val
120 125 130
ccc gat tcc cta ccc cca ttt gtc atc gca ggc acc aga atc acc caa 547
Pro Asp Ser Leu Pro Pro Phe Val Ile Ala Gly Thr Arg Ile Thr Gln
135 140 145
gcc agc acc cat gat tca gtg gcc aac gat gac ttc cgg ggc gca gaa 595
Ala Ser Thr His Asp Ser Val Ala Asn Asp Asp Phe Arg Gly Ala Glu
150 155 160 165
ata gcc aca aaa cac ctc atc gat ctt gga cac acc cac atc gcc cac 643
Ile Ala Thr Lys His Leu Ile Asp Leu Gly His Thr His Ile Ala His
170 175 180
cta cgc gtg gga agc ggc gct ggc tta cga cgc ttc gaa agc ttt gag 691
Leu Arg Val Gly Ser Gly Ala Gly Leu Arg Arg Phe Glu Ser Phe Glu
185 190 195
gca acc atg cgt gca cat ggc ctg gag ccg ctt tcc aac gat tac ctc 739
Ala Thr Met Arg Ala His Gly Leu Glu Pro Leu Ser Asn Asp Tyr Leu
200 205 210
ggc ccc gcc gtt gag cac gcc ggg tac acc gaa acc ctc gca cta ctc 787
Gly Pro Ala Val Glu His Ala Gly Tyr Thr Glu Thr Leu Ala Leu Leu
215 220 225
aaa gag cac ccg gag gtc acc gcc att ttc tcc tca aac gac atc acc 835
Lys Glu His Pro Glu Val Thr Ala Ile Phe Ser Ser Asn Asp Ile Thr
230 235 240 245
gcc atc gga gca ctc ggt gcc gcc cgt gaa cta ggt tta cgc gta cct 883
Ala Ile Gly Ala Leu Gly Ala Ala Arg Glu Leu Gly Leu Arg Val Pro
250 255 260
gaa gat cta tca ata atc gga tat gac aac act ccc ctc gcc caa acc 931
Glu Asp Leu Ser Ile Ile Gly Tyr Asp Asn Thr Pro Leu Ala Gln Thr
265 270 275
cga ctg atc aac ctc acc acc atc gac gac aac agc atc ggc gtc ggc 979
Arg Leu Ile Asn Leu Thr Thr Ile Asp Asp Asn Ser Ile Gly Val Gly
280 285 290
tac aac gcc gct ctc ttg ttg ctg agc atg ctt gat ccc gag gca ccc 1027
Tyr Asn Ala Ala Leu Leu Leu Leu Ser Met Leu Asp Pro Glu Ala Pro
295 300 305
cac ccg gag atc atg cat acg ttg cag ccc tcg ctg att gaa cgg ggc 1075
His Pro Glu Ile Met His Thr Leu Gln Pro Ser Leu Ile Glu Arg Gly
310 315 320 325
acg tgc gcg cca cgt gga tagctacccc aaatacttgg acttcctaat 1123
Thr Cys Ala Pro Arg Gly
330
<210> 32
<211> 331
<212> PRT
<213> Corynebacterium glutamicum
<400> 32
Met Ser Thr Ser Arg Pro Thr Ile Tyr Asp Val Ala Lys Ala Ala Gly
1 5 10 15
Val Ser Lys Ser Leu Val Ser Leu Val Leu Arg Gly Ser Pro Asn Val
20 25 30
Ser Lys Glu Ser Glu Ala Ala Val Lys Thr Ala Ile Lys Lys Leu Asn
35 40 45
Tyr Gln Pro Asn Arg Ala Ala Ser Asp Leu Ala Ala Lys Arg Thr Gln
50 55 60
Leu Ile Ala Val Leu Ile Asp Asp Tyr Ser Asn Pro Trp Phe Ile Asp
65 70 75 80
Leu Ile Gln Ser Leu Ser Asp Val Leu Thr Pro Lys Gly Tyr Arg Leu
85 90 95
Ser Val Ile Asp Ser Leu Thr Ser Gln Ala Gly Thr Asp Pro Ile Thr
100 105 110
Ser Ala Leu Ser Met Arg Pro Asp Gly Ile Ile Ile Ala Gln Asp Ile
115 120 125
Pro Asp Phe Thr Val Pro Asp Ser Leu Pro Pro Phe Val Ile Ala Gly
130 135 140
Thr Arg Ile Thr Gln Ala Ser Thr His Asp Ser Val Ala Asn Asp Asp
145 150 155 160
Phe Arg Gly Ala Glu Ile Ala Thr Lys His Leu Ile Asp Leu Gly His
165 170 175
Thr His Ile Ala His Leu Arg Val Gly Ser Gly Ala Gly Leu Arg Arg
180 185 190
Phe Glu Ser Phe Glu Ala Thr Met Arg Ala His Gly Leu Glu Pro Leu
195 200 205
Ser Asn Asp Tyr Leu Gly Pro Ala Val Glu His Ala Gly Tyr Thr Glu
210 215 220
Thr Leu Ala Leu Leu Lys Glu His Pro Glu Val Thr Ala Ile Phe Ser
225 230 235 240
Ser Asn Asp Ile Thr Ala Ile Gly Ala Leu Gly Ala Ala Arg Glu Leu
245 250 255
Gly Leu Arg Val Pro Glu Asp Leu Ser Ile Ile Gly Tyr Asp Asn Thr
260 265 270
Pro Leu Ala Gln Thr Arg Leu Ile Asn Leu Thr Thr Ile Asp Asp Asn
275 280 285
Ser Ile Gly Val Gly Tyr Asn Ala Ala Leu Leu Leu Leu Ser Met Leu
290 295 300
Asp Pro Glu Ala Pro His Pro Glu Ile Met His Thr Leu Gln Pro Ser
305 310 315 320
Leu Ile Glu Arg Gly Thr Cys Ala Pro Arg Gly
325 330
<210> 33
<211> 502
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(472)
<223> RXA01431
<400> 33
caccgcagct ggttccggtt gccgcgcagc gatcgatgca gagcattacc tagcttctct 60
ggcctaattc acagttagcc ttaaaccaaa ccatgtacca atg aat gtc gga ttc 115
Met Asn Val Gly Phe
1 5
ccc agg agt ccc gtc att gtt aat tta gga gaa acc atg agc aat gtt 163
Pro Arg Ser Pro Val Ile Val Asn Leu Gly Glu Thr Met Ser Asn Val
10 15 20
gtt gca gta acc gag cag acc ttc aag tcc acc gtc atc gat tcc gac 211
Val Ala Val Thr Glu Gln Thr Phe Lys Ser Thr Val Ile Asp Ser Asp
25 30 35
aag cca gtc atc gtt gac ttc tgg gca gaa tgg tgt ggc ccc tgc aag 259
Lys Pro Val Ile Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Cys Lys
40 45 50
aag ctc agc ccc atc att gag gaa atc gca ggc gag tac ggc gac aag 307
Lys Leu Ser Pro Ile Ile Glu Glu Ile Ala Gly Glu Tyr Gly Asp Lys
55 60 65
gca gtc gtt gcc agc gtc gac gtc gat gca gag cgt acc ttg ggt gcc 355
Ala Val Val Ala Ser Val Asp Val Asp Ala Glu Arg Thr Leu Gly Ala
70 75 80 85
atg ttc cag att atg tcg att cct tct gtt ctc att ttc aaa aat ggt 403
Met Phe Gln Ile Met Ser Ile Pro Ser Val Leu Ile Phe Lys Asn Gly
90 95 100
gca aaa gtc gag gaa ttt gtc ggt ctg cgc ccc aag aac gaa att gtg 451
Ala Lys Val Glu Glu Phe Val Gly Leu Arg Pro Lys Asn Glu Ile Val
105 110 115
gaa aaa cta gag aag cac ctc tagctggtat tcttactgca gtcacgtgga 502
Glu Lys Leu Glu Lys His Leu
120
<210> 34
<211> 124
<212> PRT
<213> Corynebacterium glutamicum
<400> 34
Met Asn Val Gly Phe Pro Arg Ser Pro Val Ile Val Asn Leu Gly Glu
1 5 10 15
Thr Met Ser Asn Val Val Ala Val Thr Glu Gln Thr Phe Lys Ser Thr
20 25 30
Val Ile Asp Ser Asp Lys Pro Val Ile Val Asp Phe Trp Ala Glu Trp
35 40 45
Cys Gly Pro Cys Lys Lys Leu Ser Pro Ile Ile Glu Glu Ile Ala Gly
50 55 60
Glu Tyr Gly Asp Lys Ala Val Val Ala Ser Val Asp Val Asp Ala Glu
65 70 75 80
Arg Thr Leu Gly Ala Met Phe Gln Ile Met Ser Ile Pro Ser Val Leu
85 90 95
Ile Phe Lys Asn Gly Ala Lys Val Glu Glu Phe Val Gly Leu Arg Pro
100 105 110
Lys Asn Glu Ile Val Glu Lys Leu Glu Lys His Leu
115 120
<210> 35
<211> 1495
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1465)
<223> RXA01438
<400> 35
ccattagcag tcgcaccccg ataggagtcg aatctacaag tggaaccccc gctcacatac 60
tccacatttt ttagaacccc tttaaggaat cgaactttat atg tct cgc cct ttg 115
Met Ser Arg Pro Leu
1 5
cgt gtt gcc gtt gtc ggt gca ggt cca gca gga atc tac gcg tct gat 163
Arg Val Ala Val Val Gly Ala Gly Pro Ala Gly Ile Tyr Ala Ser Asp
10 15 20
ttg ttg atg aaa tcc gac acg gac gtg cag att gat ctt ttt gaa cgt 211
Leu Leu Met Lys Ser Asp Thr Asp Val Gln Ile Asp Leu Phe Glu Arg
25 30 35
atg cca gcg cct ttc ggt ttg atc cgt tat ggt gtt gcg cct gat cac 259
Met Pro Ala Pro Phe Gly Leu Ile Arg Tyr Gly Val Ala Pro Asp His
40 45 50
cct cgc atc aag ggc atc gtg aag tcc ctg cac aat gtg atg gac aag 307
Pro Arg Ile Lys Gly Ile Val Lys Ser Leu His Asn Val Met Asp Lys
55 60 65
gag cag ctg cgt ttc ttg ggc aac att gag gtc ggc aag gac atc act 355
Glu Gln Leu Arg Phe Leu Gly Asn Ile Glu Val Gly Lys Asp Ile Thr
70 75 80 85
gtt gag gag ttg cgt gag ttt tat gac gcg atc gtg ttc tcc act ggc 403
Val Glu Glu Leu Arg Glu Phe Tyr Asp Ala Ile Val Phe Ser Thr Gly
90 95 100
gct act ggc gac cag gat ctt cgg gtt cca ggt tct gat ctg gaa ggt 451
Ala Thr Gly Asp Gln Asp Leu Arg Val Pro Gly Ser Asp Leu Glu Gly
105 110 115
tcg tgg ggc gct ggc gag ttc gtt ggt ttc tat gat ggc aac ccg aac 499
Ser Trp Gly Ala Gly Glu Phe Val Gly Phe Tyr Asp Gly Asn Pro Asn
120 125 130
ttt gaa cgc aac tgg gat ctt tct gct gag aag gta gcg gtt gtt ggt 547
Phe Glu Arg Asn Trp Asp Leu Ser Ala Glu Lys Val Ala Val Val Gly
135 140 145
gtc ggt aac gtg gcg ttg gac gtt gct cgt att ttg gcg aag act ggc 595
Val Gly Asn Val Ala Leu Asp Val Ala Arg Ile Leu Ala Lys Thr Gly
150 155 160 165
gat gag ctg cta gtt act gaa atc cct gac aat gtc tat gag agc ttg 643
Asp Glu Leu Leu Val Thr Glu Ile Pro Asp Asn Val Tyr Glu Ser Leu
170 175 180
gct aag aat cag gct aag gaa gtg cac gtt ttt ggt cgt cgt gga cct 691
Ala Lys Asn Gln Ala Lys Glu Val His Val Phe Gly Arg Arg Gly Pro
185 190 195
gct cag gcg aag ttc act ccg ttg gag ctg aag gaa ctt gac cat tcc 739
Ala Gln Ala Lys Phe Thr Pro Leu Glu Leu Lys Glu Leu Asp His Ser
200 205 210
gac acc atc gag gtg atc gtg aac cct gag gac att gat tac gat gca 787
Asp Thr Ile Glu Val Ile Val Asn Pro Glu Asp Ile Asp Tyr Asp Ala
215 220 225
gct tcg gag cag gct cgt cgt gat tcc aag tct cag gac ctc gtg tgc 835
Ala Ser Glu Gln Ala Arg Arg Asp Ser Lys Ser Gln Asp Leu Val Cys
230 235 240 245
cag act ttg gaa agc tac gcg atg cgc gat cct aag ggc gct cct cac 883
Gln Thr Leu Glu Ser Tyr Ala Met Arg Asp Pro Lys Gly Ala Pro His
250 255 260
aag ctg ttc att cac ttc ttt gag tcc cca gtg gag atc ctc ggt gag 931
Lys Leu Phe Ile His Phe Phe Glu Ser Pro Val Glu Ile Leu Gly Glu
265 270 275
gac ggc aag gtt gtt ggc ctc aag act gag cgt act cag ctg gac ggc 979
Asp Gly Lys Val Val Gly Leu Lys Thr Glu Arg Thr Gln Leu Asp Gly
280 285 290
aac ggt ggc gtg act ggc acc ggc gag ttc aag acc tgg gat atg cag 1027
Asn Gly Gly Val Thr Gly Thr Gly Glu Phe Lys Thr Trp Asp Met Gln
295 300 305
tca gtt tac cgc gcg gta ggt tac cgt tct gat gcg atc gag ggt gtt 1075
Ser Val Tyr Arg Ala Val Gly Tyr Arg Ser Asp Ala Ile Glu Gly Val
310 315 320 325
cct ttt gac gat gag cgc gcg gtt gtc ccc aac gac ggc ggc cac atc 1123
Pro Phe Asp Asp Glu Arg Ala Val Val Pro Asn Asp Gly Gly His Ile
330 335 340
atc gat cct gag gtc ggc tcc ccc atc act ggc ctg tac gcc act ggc 1171
Ile Asp Pro Glu Val Gly Ser Pro Ile Thr Gly Leu Tyr Ala Thr Gly
345 350 355
tgg atc aag cgt ggc cca att gga ctg atc ggc aac acc aag tcc gac 1219
Trp Ile Lys Arg Gly Pro Ile Gly Leu Ile Gly Asn Thr Lys Ser Asp
360 365 370
gcc aag gaa acc act gag atg ctg ctt gct gat cac gct gct ggt tct 1267
Ala Lys Glu Thr Thr Glu Met Leu Leu Ala Asp His Ala Ala Gly Ser
375 380 385
ttg cct gcg cct gca aag cct gag ttg gag tcc atc att gag ttc ctc 1315
Leu Pro Ala Pro Ala Lys Pro Glu Leu Glu Ser Ile Ile Glu Phe Leu
390 395 400 405
gat gag cgc aag gtt gcg ttc acc aca tgg gat ggc tgg cac ctg ctg 1363
Asp Glu Arg Lys Val Ala Phe Thr Thr Trp Asp Gly Trp His Leu Leu
410 415 420
gat gct gcg gag cgc gcg ctg ggt gag cct gag ggc cgc gag cgc aag 1411
Asp Ala Ala Glu Arg Ala Leu Gly Glu Pro Glu Gly Arg Glu Arg Lys
425 430 435
aag atc gtt gag tgg aat gac atg gtg cgc cat gct cgt cca gaa tac 1459
Lys Ile Val Glu Trp Asn Asp Met Val Arg His Ala Arg Pro Glu Tyr
440 445 450
gac atc taaagtcgct taaagcctca aaaaagggcg 1495
Asp Ile
455
<210> 36
<211> 455
<212> PRT
<213> Corynebacterium glutamicum
<400> 36
Met Ser Arg Pro Leu Arg Val Ala Val Val Gly Ala Gly Pro Ala Gly
1 5 10 15
Ile Tyr Ala Ser Asp Leu Leu Met Lys Ser Asp Thr Asp Val Gln Ile
20 25 30
Asp Leu Phe Glu Arg Met Pro Ala Pro Phe Gly Leu Ile Arg Tyr Gly
35 40 45
Val Ala Pro Asp His Pro Arg Ile Lys Gly Ile Val Lys Ser Leu His
50 55 60
Asn Val Met Asp Lys Glu Gln Leu Arg Phe Leu Gly Asn Ile Glu Val
65 70 75 80
Gly Lys Asp Ile Thr Val Glu Glu Leu Arg Glu Phe Tyr Asp Ala Ile
85 90 95
Val Phe Ser Thr Gly Ala Thr Gly Asp Gln Asp Leu Arg Val Pro Gly
100 105 110
Ser Asp Leu Glu Gly Ser Trp Gly Ala Gly Glu Phe Val Gly Phe Tyr
115 120 125
Asp Gly Asn Pro Asn Phe Glu Arg Asn Trp Asp Leu Ser Ala Glu Lys
130 135 140
Val Ala Val Val Gly Val Gly Asn Val Ala Leu Asp Val Ala Arg Ile
145 150 155 160
Leu Ala Lys Thr Gly Asp Glu Leu Leu Val Thr Glu Ile Pro Asp Asn
165 170 175
Val Tyr Glu Ser Leu Ala Lys Asn Gln Ala Lys Glu Val His Val Phe
180 185 190
Gly Arg Arg Gly Pro Ala Gln Ala Lys Phe Thr Pro Leu Glu Leu Lys
195 200 205
Glu Leu Asp His Ser Asp Thr Ile Glu Val Ile Val Asn Pro Glu Asp
210 215 220
Ile Asp Tyr Asp Ala Ala Ser Glu Gln Ala Arg Arg Asp Ser Lys Ser
225 230 235 240
Gln Asp Leu Val Cys Gln Thr Leu Glu Ser Tyr Ala Met Arg Asp Pro
245 250 255
Lys Gly Ala Pro His Lys Leu Phe Ile His Phe Phe Glu Ser Pro Val
260 265 270
Glu Ile Leu Gly Glu Asp Gly Lys Val Val Gly Leu Lys Thr Glu Arg
275 280 285
Thr Gln Leu Asp Gly Asn Gly Gly Val Thr Gly Thr Gly Glu Phe Lys
290 295 300
Thr Trp Asp Met Gln Ser Val Tyr Arg Ala Val Gly Tyr Arg Ser Asp
305 310 315 320
Ala Ile Glu Gly Val Pro Phe Asp Asp Glu Arg Ala Val Val Pro Asn
325 330 335
Asp Gly Gly His Ile Ile Asp Pro Glu Val Gly Ser Pro Ile Thr Gly
340 345 350
Leu Tyr Ala Thr Gly Trp Ile Lys Arg Gly Pro Ile Gly Leu Ile Gly
355 360 365
Asn Thr Lys Ser Asp Ala Lys Glu Thr Thr Glu Met Leu Leu Ala Asp
370 375 380
His Ala Ala Gly Ser Leu Pro Ala Pro Ala Lys Pro Glu Leu Glu Ser
385 390 395 400
Ile Ile Glu Phe Leu Asp Glu Arg Lys Val Ala Phe Thr Thr Trp Asp
405 410 415
Gly Trp His Leu Leu Asp Ala Ala Glu Arg Ala Leu Gly Glu Pro Glu
420 425 430
Gly Arg Glu Arg Lys Lys Ile Val Glu Trp Asn Asp Met Val Arg His
435 440 445
Ala Arg Pro Glu Tyr Asp Ile
450 455
<210> 37
<211> 1021
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(991)
<223> RXA01490
<400> 37
cacaccaatg gtgactactg atccttgaag atcagccgga acgctgtcta gtccactcca 60
aatatccact gttttagact acggcataga ctcaacagac atg aat gct cct gcc 115
Met Asn Ala Pro Ala
1 5
cct aaa cct gga ctc gtg atc gtc gac aag ccc gcc gga atg aca tcc 163
Pro Lys Pro Gly Leu Val Ile Val Asp Lys Pro Ala Gly Met Thr Ser
10 15 20
cat gac gtg gtg tcc aaa ttg cgc cgc gca ttt tcc acc cgc aaa gta 211
His Asp Val Val Ser Lys Leu Arg Arg Ala Phe Ser Thr Arg Lys Val
25 30 35
ggc cac gca ggc acc ctc gac ccc atg gca acc ggc gtg tta gtc gtc 259
Gly His Ala Gly Thr Leu Asp Pro Met Ala Thr Gly Val Leu Val Val
40 45 50
gga att gag cgc gga acc cgc ttc ctg gca cac atg gtg gcc tcc acc 307
Gly Ile Glu Arg Gly Thr Arg Phe Leu Ala His Met Val Ala Ser Thr
55 60 65
aaa gcc tac gac gcc acc att cga ctc ggc gcc gcc acc agc acc gat 355
Lys Ala Tyr Asp Ala Thr Ile Arg Leu Gly Ala Ala Thr Ser Thr Asp
70 75 80 85
gat gca gaa ggc gag gtt atc tcc aca aca gac gca tcc ggc ctc gac 403
Asp Ala Glu Gly Glu Val Ile Ser Thr Thr Asp Ala Ser Gly Leu Asp
90 95 100
cac agc acc atc ctt gct gaa atc gtc aac ctc acc ggc gac atc atg 451
His Ser Thr Ile Leu Ala Glu Ile Val Asn Leu Thr Gly Asp Ile Met
105 110 115
caa aaa ccc acc aaa gtc tcc gcc atc aaa atc gac ggc aaa cgc gcc 499
Gln Lys Pro Thr Lys Val Ser Ala Ile Lys Ile Asp Gly Lys Arg Ala
120 125 130
cac gaa cgc gtc cgc gac ggc gaa gaa gta gac att ccc gca cgt ccc 547
His Glu Arg Val Arg Asp Gly Glu Glu Val Asp Ile Pro Ala Arg Pro
135 140 145
gtc acc gtc agc gtc ttt gac gtg ctc gac tac cac gtc gac ggt gaa 595
Val Thr Val Ser Val Phe Asp Val Leu Asp Tyr His Val Asp Gly Glu
150 155 160 165
ttt tat gac tta gat gtg cgc gtc cac tgc tcc tcc ggc acc tac atc 643
Phe Tyr Asp Leu Asp Val Arg Val His Cys Ser Ser Gly Thr Tyr Ile
170 175 180
cgc gcg ctc gcc cgc gac ctc ggc aac gct ttg cag gtc ggc ggc cac 691
Arg Ala Leu Ala Arg Asp Leu Gly Asn Ala Leu Gln Val Gly Gly His
185 190 195
ctg acc gcg ctt agg cgc aca gag gtc ggc cct ttt acg ctt aac gac 739
Leu Thr Ala Leu Arg Arg Thr Glu Val Gly Pro Phe Thr Leu Asn Asp
200 205 210
gcg acc ccc ctc tcc aaa ctc caa gag aat cca gaa ctc tcc ctc aac 787
Ala Thr Pro Leu Ser Lys Leu Gln Glu Asn Pro Glu Leu Ser Leu Asn
215 220 225
ctc gac cag gca ctc acc cgc agt tac cca gtc ctt gac atc acc gaa 835
Leu Asp Gln Ala Leu Thr Arg Ser Tyr Pro Val Leu Asp Ile Thr Glu
230 235 240 245
gac gaa ggc gtt gac ctg tcc atg ggc aaa tgg ttg gaa cct cgc gga 883
Asp Glu Gly Val Asp Leu Ser Met Gly Lys Trp Leu Glu Pro Arg Gly
250 255 260
ctg aaa ggc gtc cac gct gca gta aca cca tca gga aaa gcc gtg gcg 931
Leu Lys Gly Val His Ala Ala Val Thr Pro Ser Gly Lys Ala Val Ala
265 270 275
ctc atc gaa gaa aag ggc aaa cgc ctg gcc acc gtg ttt gtt gct cac 979
Leu Ile Glu Glu Lys Gly Lys Arg Leu Ala Thr Val Phe Val Ala His
280 285 290
ccc aac act ctt tagttggtct gccagaagcc gatttaagag 1021
Pro Asn Thr Leu
295
<210> 38
<211> 297
<212> PRT
<213> Corynebacterium glutamicum
<400> 38
Met Asn Ala Pro Ala Pro Lys Pro Gly Leu Val Ile Val Asp Lys Pro
1 5 10 15
Ala Gly Met Thr Ser His Asp Val Val Ser Lys Leu Arg Arg Ala Phe
20 25 30
Ser Thr Arg Lys Val Gly His Ala Gly Thr Leu Asp Pro Met Ala Thr
35 40 45
Gly Val Leu Val Val Gly Ile Glu Arg Gly Thr Arg Phe Leu Ala His
50 55 60
Met Val Ala Ser Thr Lys Ala Tyr Asp Ala Thr Ile Arg Leu Gly Ala
65 70 75 80
Ala Thr Ser Thr Asp Asp Ala Glu Gly Glu Val Ile Ser Thr Thr Asp
85 90 95
Ala Ser Gly Leu Asp His Ser Thr Ile Leu Ala Glu Ile Val Asn Leu
100 105 110
Thr Gly Asp Ile Met Gln Lys Pro Thr Lys Val Ser Ala Ile Lys Ile
115 120 125
Asp Gly Lys Arg Ala His Glu Arg Val Arg Asp Gly Glu Glu Val Asp
130 135 140
Ile Pro Ala Arg Pro Val Thr Val Ser Val Phe Asp Val Leu Asp Tyr
145 150 155 160
His Val Asp Gly Glu Phe Tyr Asp Leu Asp Val Arg Val His Cys Ser
165 170 175
Ser Gly Thr Tyr Ile Arg Ala Leu Ala Arg Asp Leu Gly Asn Ala Leu
180 185 190
Gln Val Gly Gly His Leu Thr Ala Leu Arg Arg Thr Glu Val Gly Pro
195 200 205
Phe Thr Leu Asn Asp Ala Thr Pro Leu Ser Lys Leu Gln Glu Asn Pro
210 215 220
Glu Leu Ser Leu Asn Leu Asp Gln Ala Leu Thr Arg Ser Tyr Pro Val
225 230 235 240
Leu Asp Ile Thr Glu Asp Glu Gly Val Asp Leu Ser Met Gly Lys Trp
245 250 255
Leu Glu Pro Arg Gly Leu Lys Gly Val His Ala Ala Val Thr Pro Ser
260 265 270
Gly Lys Ala Val Ala Leu Ile Glu Glu Lys Gly Lys Arg Leu Ala Thr
275 280 285
Val Phe Val Ala His Pro Asn Thr Leu
290 295
<210> 39
<211> 1441
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1411)
<223> RXA01493
<400> 39
cctgctgcag gctataccgc tcgtggtacg gaaatcgaag ccctcgatac gttgattgaa 60
gcaaccgtta ccttggggga gtctttgcga agctcggcgc atg tcg atg tct aac 115
Met Ser Met Ser Asn
1 5
aac gac ttt gag cat gag tcc cat gat gtt tct gca aag cag atc ttc 163
Asn Asp Phe Glu His Glu Ser His Asp Val Ser Ala Lys Gln Ile Phe
10 15 20
ggg ctc gcg ttc ccc gca ctg ggt gtt cta gct gcg atg ccg ctg tat 211
Gly Leu Ala Phe Pro Ala Leu Gly Val Leu Ala Ala Met Pro Leu Tyr
25 30 35
ctc ttg ttg gat aca gcg gtt gtt ggc act ttg ggt ggc ttc gaa ttg 259
Leu Leu Leu Asp Thr Ala Val Val Gly Thr Leu Gly Gly Phe Glu Leu
40 45 50
gct gcg ttg ggc gca gca aca aca att caa gct caa gtg aca aca cag 307
Ala Ala Leu Gly Ala Ala Thr Thr Ile Gln Ala Gln Val Thr Thr Gln
55 60 65
ctg aca ttc ttg tcc tat gga act acc gcg aga tca tcg aga att ttc 355
Leu Thr Phe Leu Ser Tyr Gly Thr Thr Ala Arg Ser Ser Arg Ile Phe
70 75 80 85
gga atg ggt gat cgc cgg gga gca att gcc gaa ggt gtg caa gca acc 403
Gly Met Gly Asp Arg Arg Gly Ala Ile Ala Glu Gly Val Gln Ala Thr
90 95 100
tgg gtg gca ctc ttt gta ggc ttg ggc atc tta acg ctg atg ctc att 451
Trp Val Ala Leu Phe Val Gly Leu Gly Ile Leu Thr Leu Met Leu Ile
105 110 115
gga gcc ccg act ttc gcg ttg tgg ctc agt ggt gat gaa gct cta gcc 499
Gly Ala Pro Thr Phe Ala Leu Trp Leu Ser Gly Asp Glu Ala Leu Ala
120 125 130
caa gaa gca ggg cat tgg ctc cgg gtc gct gct ttt gcg gtg cca cta 547
Gln Glu Ala Gly His Trp Leu Arg Val Ala Ala Phe Ala Val Pro Leu
135 140 145
att ctc atg atc atg gct ggc aac ggt tgg tta aga ggt att caa aac 595
Ile Leu Met Ile Met Ala Gly Asn Gly Trp Leu Arg Gly Ile Gln Asn
150 155 160 165
acc aag ctg cca ctc tat ttc acc ttg gcg gga gtc atc ccc ggc gcg 643
Thr Lys Leu Pro Leu Tyr Phe Thr Leu Ala Gly Val Ile Pro Gly Ala
170 175 180
atc ttg att ccg ata ttc gtg gct aag ttt gga ctt gtg ggc tct gcc 691
Ile Leu Ile Pro Ile Phe Val Ala Lys Phe Gly Leu Val Gly Ser Ala
185 190 195
tgg gca aac ctc att gca gaa gca att act gct tcg ctg ttt ttg ggt 739
Trp Ala Asn Leu Ile Ala Glu Ala Ile Thr Ala Ser Leu Phe Leu Gly
200 205 210
gca ttg atc aag cac cac gaa ggt tcg tgg aag ccg agc tgg acg gtg 787
Ala Leu Ile Lys His His Glu Gly Ser Trp Lys Pro Ser Trp Thr Val
215 220 225
atg aaa aat cag ttg gtt ctt gga cgt gat ttg atc atg cgg tca atg 835
Met Lys Asn Gln Leu Val Leu Gly Arg Asp Leu Ile Met Arg Ser Met
230 235 240 245
tcg ttc cag gtt gct ttt ctt tcc gcg gcc gct gtg gct gca cga ttt 883
Ser Phe Gln Val Ala Phe Leu Ser Ala Ala Ala Val Ala Ala Arg Phe
250 255 260
ggc acg gca tcc ttg gcg gcc cac cag gtg ttg ctt cag ctg tgg aat 931
Gly Thr Ala Ser Leu Ala Ala His Gln Val Leu Leu Gln Leu Trp Asn
265 270 275
ttc atc aca ttg gtg ctg gat tct cta gct atc gcg gcg cag acc tta 979
Phe Ile Thr Leu Val Leu Asp Ser Leu Ala Ile Ala Ala Gln Thr Leu
280 285 290
act ggt gca gcc ctg ggc gct gga act gcg aag gtc gcc cgc agg gtg 1027
Thr Gly Ala Ala Leu Gly Ala Gly Thr Ala Lys Val Ala Arg Arg Val
295 300 305
ggt aat cag gtg att aag tac tct ctg att ttc gct ggt ggc tta ggt 1075
Gly Asn Gln Val Ile Lys Tyr Ser Leu Ile Phe Ala Gly Gly Leu Gly
310 315 320 325
ttg gtg ttc gtg gtc tta cac tcg tgg att ccg cgt att ttc act cag 1123
Leu Val Phe Val Val Leu His Ser Trp Ile Pro Arg Ile Phe Thr Gln
330 335 340
gac gcc gac gtt tta gat gcg att gct tcc ccg tgg tgg atc atg gtc 1171
Asp Ala Asp Val Leu Asp Ala Ile Ala Ser Pro Trp Trp Ile Met Val
345 350 355
gcg atg atc att ttg ggt ggc att gtc ttt gct att gat ggt gtg ctg 1219
Ala Met Ile Ile Leu Gly Gly Ile Val Phe Ala Ile Asp Gly Val Leu
360 365 370
ttg ggt gct gct gac gcg gtg ttc ctc cga aat gcc tct atc ttg gcg 1267
Leu Gly Ala Ala Asp Ala Val Phe Leu Arg Asn Ala Ser Ile Leu Ala
375 380 385
gtt gtg gtc gga ttc tta cca ggc gtc tgg att tcc tat gca tta gat 1315
Val Val Val Gly Phe Leu Pro Gly Val Trp Ile Ser Tyr Ala Leu Asp
390 395 400 405
gca ggg ctg aca ggc gtg tgg tgt ggt ttg ctg gcg ttt att ctg atc 1363
Ala Gly Leu Thr Gly Val Trp Cys Gly Leu Leu Ala Phe Ile Leu Ile
410 415 420
cga cta ttt gcg gtg att tgg cgg ttt aag tct atg aag tgg gcg cgt 1411
Arg Leu Phe Ala Val Ile Trp Arg Phe Lys Ser Met Lys Trp Ala Arg
425 430 435
tagcttcggc gcgtggcaaa ccacatttgc 1441
<210> 40
<211> 437
<212> PRT
<213> Corynebacterium glutamicum
<400> 40
Met Ser Met Ser Asn Asn Asp Phe Glu His Glu Ser His Asp Val Ser
1 5 10 15
Ala Lys Gln Ile Phe Gly Leu Ala Phe Pro Ala Leu Gly Val Leu Ala
20 25 30
Ala Met Pro Leu Tyr Leu Leu Leu Asp Thr Ala Val Val Gly Thr Leu
35 40 45
Gly Gly Phe Glu Leu Ala Ala Leu Gly Ala Ala Thr Thr Ile Gln Ala
50 55 60
Gln Val Thr Thr Gln Leu Thr Phe Leu Ser Tyr Gly Thr Thr Ala Arg
65 70 75 80
Ser Ser Arg Ile Phe Gly Met Gly Asp Arg Arg Gly Ala Ile Ala Glu
85 90 95
Gly Val Gln Ala Thr Trp Val Ala Leu Phe Val Gly Leu Gly Ile Leu
100 105 110
Thr Leu Met Leu Ile Gly Ala Pro Thr Phe Ala Leu Trp Leu Ser Gly
115 120 125
Asp Glu Ala Leu Ala Gln Glu Ala Gly His Trp Leu Arg Val Ala Ala
130 135 140
Phe Ala Val Pro Leu Ile Leu Met Ile Met Ala Gly Asn Gly Trp Leu
145 150 155 160
Arg Gly Ile Gln Asn Thr Lys Leu Pro Leu Tyr Phe Thr Leu Ala Gly
165 170 175
Val Ile Pro Gly Ala Ile Leu Ile Pro Ile Phe Val Ala Lys Phe Gly
180 185 190
Leu Val Gly Ser Ala Trp Ala Asn Leu Ile Ala Glu Ala Ile Thr Ala
195 200 205
Ser Leu Phe Leu Gly Ala Leu Ile Lys His His Glu Gly Ser Trp Lys
210 215 220
Pro Ser Trp Thr Val Met Lys Asn Gln Leu Val Leu Gly Arg Asp Leu
225 230 235 240
Ile Met Arg Ser Met Ser Phe Gln Val Ala Phe Leu Ser Ala Ala Ala
245 250 255
Val Ala Ala Arg Phe Gly Thr Ala Ser Leu Ala Ala His Gln Val Leu
260 265 270
Leu Gln Leu Trp Asn Phe Ile Thr Leu Val Leu Asp Ser Leu Ala Ile
275 280 285
Ala Ala Gln Thr Leu Thr Gly Ala Ala Leu Gly Ala Gly Thr Ala Lys
290 295 300
Val Ala Arg Arg Val Gly Asn Gln Val Ile Lys Tyr Ser Leu Ile Phe
305 310 315 320
Ala Gly Gly Leu Gly Leu Val Phe Val Val Leu His Ser Trp Ile Pro
325 330 335
Arg Ile Phe Thr Gln Asp Ala Asp Val Leu Asp Ala Ile Ala Ser Pro
340 345 350
Trp Trp Ile Met Val Ala Met Ile Ile Leu Gly Gly Ile Val Phe Ala
355 360 365
Ile Asp Gly Val Leu Leu Gly Ala Ala Asp Ala Val Phe Leu Arg Asn
370 375 380
Ala Ser Ile Leu Ala Val Val Val Gly Phe Leu Pro Gly Val Trp Ile
385 390 395 400
Ser Tyr Ala Leu Asp Ala Gly Leu Thr Gly Val Trp Cys Gly Leu Leu
405 410 415
Ala Phe Ile Leu Ile Arg Leu Phe Ala Val Ile Trp Arg Phe Lys Ser
420 425 430
Met Lys Trp Ala Arg
435
<210> 41
<211> 2056
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2026)
<223> RXA01559
<400> 41
ctttctcgct cgtgtcgcac tcacccacgc cacctggcgt gggtgagtgg cgcatggagt 60
gggtgggcgt cgacaagcgt ggttgtctgg ttgattggaa ttg aag gag act ttc 115
Leu Lys Glu Thr Phe
1 5
ttg gct cgg caa aaa aag agt gcc gct agc gcc tgg gaa cga tgg cca 163
Leu Ala Arg Gln Lys Lys Ser Ala Ala Ser Ala Trp Glu Arg Trp Pro
10 15 20
aaa cgc gca ata gcg ttg ttt gtg ctc atc gtc gtt ggt gtt tat gcg 211
Lys Arg Ala Ile Ala Leu Phe Val Leu Ile Val Val Gly Val Tyr Ala
25 30 35
ttg gtg ctg ttg aca ggc gat cgt tct gcc aca cca aaa ttg ggt att 259
Leu Val Leu Leu Thr Gly Asp Arg Ser Ala Thr Pro Lys Leu Gly Ile
40 45 50
gat ctg caa ggc gga acc cga gtg acc ctc gtg ccg cag ggg cag gat 307
Asp Leu Gln Gly Gly Thr Arg Val Thr Leu Val Pro Gln Gly Gln Asp
55 60 65
cca act cag gac cag ctg aat cag gca cgc acc att ctg gaa aac cgt 355
Pro Thr Gln Asp Gln Leu Asn Gln Ala Arg Thr Ile Leu Glu Asn Arg
70 75 80 85
gtg aac ggc atg ggc gtt tca ggt gca agc gtg gtc gct gac ggt aac 403
Val Asn Gly Met Gly Val Ser Gly Ala Ser Val Val Ala Asp Gly Asn
90 95 100
acg ctg gtg atc act gtt ccc ggg gaa aat acc gca cag gcg caa tcc 451
Thr Leu Val Ile Thr Val Pro Gly Glu Asn Thr Ala Gln Ala Gln Ser
105 110 115
cta gga cag acc tcc cag ctg ctg ttc cgt ccc gtt ggt cag gca gga 499
Leu Gly Gln Thr Ser Gln Leu Leu Phe Arg Pro Val Gly Gln Ala Gly
120 125 130
atg ccc gat atg acc acg ttg atg cca gag ctg gaa gag atg gcc aac 547
Met Pro Asp Met Thr Thr Leu Met Pro Glu Leu Glu Glu Met Ala Asn
135 140 145
agg tgg gtt gaa tac ggc gtc atc acc gaa gag cag gca aat gcc tcc 595
Arg Trp Val Glu Tyr Gly Val Ile Thr Glu Glu Gln Ala Asn Ala Ser
150 155 160 165
ttg gag gaa atg aac acc gct gtt gca tcg acc act gcg gtg gaa ggc 643
Leu Glu Glu Met Asn Thr Ala Val Ala Ser Thr Thr Ala Val Glu Gly
170 175 180
gaa gaa gca act gag cca gaa ccc gtc acc gtg tcg gcg acc cct atg 691
Glu Glu Ala Thr Glu Pro Glu Pro Val Thr Val Ser Ala Thr Pro Met
185 190 195
gat gag cca gcc aac tcc att gag gca aca cag cga cgc cag gaa atc 739
Asp Glu Pro Ala Asn Ser Ile Glu Ala Thr Gln Arg Arg Gln Glu Ile
200 205 210
acg gac atg ctg cgc acc gac cgc cag tcc acc gat ccc act gtc cag 787
Thr Asp Met Leu Arg Thr Asp Arg Gln Ser Thr Asp Pro Thr Val Gln
215 220 225
atc gct gca agt tct ttg atg cag tgc acc act gat gag atg gat cct 835
Ile Ala Ala Ser Ser Leu Met Gln Cys Thr Thr Asp Glu Met Asp Pro
230 235 240 245
ttg gcc ggc acc gat gat cca cgc ctg cca ttg gtg gca tgt gat cca 883
Leu Ala Gly Thr Asp Asp Pro Arg Leu Pro Leu Val Ala Cys Asp Pro
250 255 260
gct gta ggt ggc gtg tat gta ctt gat cct gca cct ttg ctc aac ggc 931
Ala Val Gly Gly Val Tyr Val Leu Asp Pro Ala Pro Leu Leu Asn Gly
265 270 275
gaa acc gat gag gaa aat ggt gcg cgc cta acc ggt aat gag atc gat 979
Glu Thr Asp Glu Glu Asn Gly Ala Arg Leu Thr Gly Asn Glu Ile Asp
280 285 290
acc aac cgt ccc atc acc ggt gga ttc aac gcc cag tcc ggc cag atg 1027
Thr Asn Arg Pro Ile Thr Gly Gly Phe Asn Ala Gln Ser Gly Gln Met
295 300 305
gaa atc agc ttt gcc ttc aaa tcc ggc gat ggg gaa gaa ggc tct gca 1075
Glu Ile Ser Phe Ala Phe Lys Ser Gly Asp Gly Glu Glu Gly Ser Ala
310 315 320 325
act tgg tcc tct ctg acc agc cag tac ctg cag cag cag atc gcc atc 1123
Thr Trp Ser Ser Leu Thr Ser Gln Tyr Leu Gln Gln Gln Ile Ala Ile
330 335 340
acc ctg gac tct cag gtg att tct gca ccc gtg att cag tca gca acc 1171
Thr Leu Asp Ser Gln Val Ile Ser Ala Pro Val Ile Gln Ser Ala Thr
345 350 355
cct gtg ggt tct gca aca tcc atc acc ggt gac ttc act caa act gaa 1219
Pro Val Gly Ser Ala Thr Ser Ile Thr Gly Asp Phe Thr Gln Thr Glu
360 365 370
gcc caa gat ctg gcg aac aac ctg cgc tac ggt gca ttg ccc ctg agc 1267
Ala Gln Asp Leu Ala Asn Asn Leu Arg Tyr Gly Ala Leu Pro Leu Ser
375 380 385
ttc gca ggt gaa aac ggc gag cgc ggc gga act acc acc acc gtt ccg 1315
Phe Ala Gly Glu Asn Gly Glu Arg Gly Gly Thr Thr Thr Thr Val Pro
390 395 400 405
cca tca cta ggc gca gca tcc ttg aag gcc gga ctg atc gca ggc atc 1363
Pro Ser Leu Gly Ala Ala Ser Leu Lys Ala Gly Leu Ile Ala Gly Ile
410 415 420
gtc ggc atc gcg ctg gtc gcc atc ttc gtg ttc gcc tac tac cgc gtc 1411
Val Gly Ile Ala Leu Val Ala Ile Phe Val Phe Ala Tyr Tyr Arg Val
425 430 435
ttc gga ttc gtt tcc ctg ttc acc ctg ttt gcc gca ggc gtg ttg gtc 1459
Phe Gly Phe Val Ser Leu Phe Thr Leu Phe Ala Ala Gly Val Leu Val
440 445 450
tac ggc ctt ctg gta ctg ctg gga cgc tgg atc gga tat tcc cta gac 1507
Tyr Gly Leu Leu Val Leu Leu Gly Arg Trp Ile Gly Tyr Ser Leu Asp
455 460 465
ctt gct ggt atc gcc ggt ttg atc atc ggt atc ggt acc acc gcc gac 1555
Leu Ala Gly Ile Ala Gly Leu Ile Ile Gly Ile Gly Thr Thr Ala Asp
470 475 480 485
tcc ttc gtg gtg ttc tat gag cgc atc aag gat gag atc cgt gaa gga 1603
Ser Phe Val Val Phe Tyr Glu Arg Ile Lys Asp Glu Ile Arg Glu Gly
490 495 500
aga tcc ttt aga tct gca gta cct cgt gca tgg gaa agc gcc aag cgc 1651
Arg Ser Phe Arg Ser Ala Val Pro Arg Ala Trp Glu Ser Ala Lys Arg
505 510 515
acc atc gtc aca ggc aac atg gtc act ttg ctc ggc gct atc gtg att 1699
Thr Ile Val Thr Gly Asn Met Val Thr Leu Leu Gly Ala Ile Val Ile
520 525 530
tac ttg ctc gcg gtc ggc gaa gtc aag ggc ttt gcc ttc acc ctg ggt 1747
Tyr Leu Leu Ala Val Gly Glu Val Lys Gly Phe Ala Phe Thr Leu Gly
535 540 545
ctg acc acc gta ttc gat ctc gtt gtc acc ttc ctg atc acg gca cca 1795
Leu Thr Thr Val Phe Asp Leu Val Val Thr Phe Leu Ile Thr Ala Pro
550 555 560 565
ctg gtt atc ctg gca tca cgc aac cca ttc ttt gcc aag tca tcg gtc 1843
Leu Val Ile Leu Ala Ser Arg Asn Pro Phe Phe Ala Lys Ser Ser Val
570 575 580
aac ggc atg gga cga gtg atg aag ctc gtt gaa gaa cgc cgc gcc aac 1891
Asn Gly Met Gly Arg Val Met Lys Leu Val Glu Glu Arg Arg Ala Asn
585 590 595
ggt gaa ttg gat gag cct gag tac ctg aaa aag atc cat gcc aag aat 1939
Gly Glu Leu Asp Glu Pro Glu Tyr Leu Lys Lys Ile His Ala Lys Asn
600 605 610
gcg gca gct gat aag gct tcc act gac aat tct tcc act gac aat tct 1987
Ala Ala Ala Asp Lys Ala Ser Thr Asp Asn Ser Ser Thr Asp Asn Ser
615 620 625
gaa gca cct ggc acc gat acg aac caa gag gag gag aag tagccatgac 2036
Glu Ala Pro Gly Thr Asp Thr Asn Gln Glu Glu Glu Lys
630 635 640
tgattcccag actgaatcac 2056
<210> 42
<211> 642
<212> PRT
<213> Corynebacterium glutamicum
<400> 42
Leu Lys Glu Thr Phe Leu Ala Arg Gln Lys Lys Ser Ala Ala Ser Ala
1 5 10 15
Trp Glu Arg Trp Pro Lys Arg Ala Ile Ala Leu Phe Val Leu Ile Val
20 25 30
Val Gly Val Tyr Ala Leu Val Leu Leu Thr Gly Asp Arg Ser Ala Thr
35 40 45
Pro Lys Leu Gly Ile Asp Leu Gln Gly Gly Thr Arg Val Thr Leu Val
50 55 60
Pro Gln Gly Gln Asp Pro Thr Gln Asp Gln Leu Asn Gln Ala Arg Thr
65 70 75 80
Ile Leu Glu Asn Arg Val Asn Gly Met Gly Val Ser Gly Ala Ser Val
85 90 95
Val Ala Asp Gly Asn Thr Leu Val Ile Thr Val Pro Gly Glu Asn Thr
100 105 110
Ala Gln Ala Gln Ser Leu Gly Gln Thr Ser Gln Leu Leu Phe Arg Pro
115 120 125
Val Gly Gln Ala Gly Met Pro Asp Met Thr Thr Leu Met Pro Glu Leu
130 135 140
Glu Glu Met Ala Asn Arg Trp Val Glu Tyr Gly Val Ile Thr Glu Glu
145 150 155 160
Gln Ala Asn Ala Ser Leu Glu Glu Met Asn Thr Ala Val Ala Ser Thr
165 170 175
Thr Ala Val Glu Gly Glu Glu Ala Thr Glu Pro Glu Pro Val Thr Val
180 185 190
Ser Ala Thr Pro Met Asp Glu Pro Ala Asn Ser Ile Glu Ala Thr Gln
195 200 205
Arg Arg Gln Glu Ile Thr Asp Met Leu Arg Thr Asp Arg Gln Ser Thr
210 215 220
Asp Pro Thr Val Gln Ile Ala Ala Ser Ser Leu Met Gln Cys Thr Thr
225 230 235 240
Asp Glu Met Asp Pro Leu Ala Gly Thr Asp Asp Pro Arg Leu Pro Leu
245 250 255
Val Ala Cys Asp Pro Ala Val Gly Gly Val Tyr Val Leu Asp Pro Ala
260 265 270
Pro Leu Leu Asn Gly Glu Thr Asp Glu Glu Asn Gly Ala Arg Leu Thr
275 280 285
Gly Asn Glu Ile Asp Thr Asn Arg Pro Ile Thr Gly Gly Phe Asn Ala
290 295 300
Gln Ser Gly Gln Met Glu Ile Ser Phe Ala Phe Lys Ser Gly Asp Gly
305 310 315 320
Glu Glu Gly Ser Ala Thr Trp Ser Ser Leu Thr Ser Gln Tyr Leu Gln
325 330 335
Gln Gln Ile Ala Ile Thr Leu Asp Ser Gln Val Ile Ser Ala Pro Val
340 345 350
Ile Gln Ser Ala Thr Pro Val Gly Ser Ala Thr Ser Ile Thr Gly Asp
355 360 365
Phe Thr Gln Thr Glu Ala Gln Asp Leu Ala Asn Asn Leu Arg Tyr Gly
370 375 380
Ala Leu Pro Leu Ser Phe Ala Gly Glu Asn Gly Glu Arg Gly Gly Thr
385 390 395 400
Thr Thr Thr Val Pro Pro Ser Leu Gly Ala Ala Ser Leu Lys Ala Gly
405 410 415
Leu Ile Ala Gly Ile Val Gly Ile Ala Leu Val Ala Ile Phe Val Phe
420 425 430
Ala Tyr Tyr Arg Val Phe Gly Phe Val Ser Leu Phe Thr Leu Phe Ala
435 440 445
Ala Gly Val Leu Val Tyr Gly Leu Leu Val Leu Leu Gly Arg Trp Ile
450 455 460
Gly Tyr Ser Leu Asp Leu Ala Gly Ile Ala Gly Leu Ile Ile Gly Ile
465 470 475 480
Gly Thr Thr Ala Asp Ser Phe Val Val Phe Tyr Glu Arg Ile Lys Asp
485 490 495
Glu Ile Arg Glu Gly Arg Ser Phe Arg Ser Ala Val Pro Arg Ala Trp
500 505 510
Glu Ser Ala Lys Arg Thr Ile Val Thr Gly Asn Met Val Thr Leu Leu
515 520 525
Gly Ala Ile Val Ile Tyr Leu Leu Ala Val Gly Glu Val Lys Gly Phe
530 535 540
Ala Phe Thr Leu Gly Leu Thr Thr Val Phe Asp Leu Val Val Thr Phe
545 550 555 560
Leu Ile Thr Ala Pro Leu Val Ile Leu Ala Ser Arg Asn Pro Phe Phe
565 570 575
Ala Lys Ser Ser Val Asn Gly Met Gly Arg Val Met Lys Leu Val Glu
580 585 590
Glu Arg Arg Ala Asn Gly Glu Leu Asp Glu Pro Glu Tyr Leu Lys Lys
595 600 605
Ile His Ala Lys Asn Ala Ala Ala Asp Lys Ala Ser Thr Asp Asn Ser
610 615 620
Ser Thr Asp Asn Ser Glu Ala Pro Gly Thr Asp Thr Asn Gln Glu Glu
625 630 635 640
Glu Lys
<210> 43
<211> 1909
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1879)
<223> RXA01596
<400> 43
tcccaggtca gcggggtaat tcgaaaacca ttcgaacaat tttcgaggat ttagaaaaaa 60
cgttcgcata aattgttaga actgatgtac actttgaggc atg ctc gta gac att 115
Met Leu Val Asp Ile
1 5
gct att gag aac ctc gga gtt att cca gcg gcc tca gct gag ttc agc 163
Ala Ile Glu Asn Leu Gly Val Ile Pro Ala Ala Ser Ala Glu Phe Ser
10 15 20
tca ggt tta aca gtg ctc acc ggt gag acc ggc gcc gga aag acc atg 211
Ser Gly Leu Thr Val Leu Thr Gly Glu Thr Gly Ala Gly Lys Thr Met
25 30 35
gta gtg aca ggt tta cgc ctg tta tcc ggc ggt cgc gcc gac gct tca 259
Val Val Thr Gly Leu Arg Leu Leu Ser Gly Gly Arg Ala Asp Ala Ser
40 45 50
cgc gtg cgc aca gga tcc cct caa gct gtt gtg gag ggg cgc ttt gtt 307
Arg Val Arg Thr Gly Ser Pro Gln Ala Val Val Glu Gly Arg Phe Val
55 60 65
acg caa ggc gtg ccc tgc gac att gtc gaa cgt gca acc gga atc gtt 355
Thr Gln Gly Val Pro Cys Asp Ile Val Glu Arg Ala Thr Gly Ile Val
70 75 80 85
tcg aac gcc gga ggt gcc gca gat gaa aat gga gag ttt tta gct gtc 403
Ser Asn Ala Gly Gly Ala Ala Asp Glu Asn Gly Glu Phe Leu Ala Val
90 95 100
cgt tcc gtc ggc gcc aac ggc cgt tca aaa gct cat ctc ggt ggt cgc 451
Arg Ser Val Gly Ala Asn Gly Arg Ser Lys Ala His Leu Gly Gly Arg
105 110 115
tcc gta cct gcg gca acg ctg tcc gag ttc tct gat gag ctg ttg acc 499
Ser Val Pro Ala Ala Thr Leu Ser Glu Phe Ser Asp Glu Leu Leu Thr
120 125 130
atc cac ggt caa aat gac caa ctc cgg ttg ctc tcc cca gaa cgc caa 547
Ile His Gly Gln Asn Asp Gln Leu Arg Leu Leu Ser Pro Glu Arg Gln
135 140 145
cta gag gcg ctt gat cgt ttt gat cca gag ctg gcc caa ctg cgc aaa 595
Leu Glu Ala Leu Asp Arg Phe Asp Pro Glu Leu Ala Gln Leu Arg Lys
150 155 160 165
aac tac aac gcc aag tac ctc act tgg aag tcc ttg gat aaa gat ctg 643
Asn Tyr Asn Ala Lys Tyr Leu Thr Trp Lys Ser Leu Asp Lys Asp Leu
170 175 180
cag aag cgc ctg agt agt agg cga gag ctg gct caa gaa gtc gat cgc 691
Gln Lys Arg Leu Ser Ser Arg Arg Glu Leu Ala Gln Glu Val Asp Arg
185 190 195
ctg caa ttc gcg att aat gag atc gag gaa gtc tcg cca cag cca ggc 739
Leu Gln Phe Ala Ile Asn Glu Ile Glu Glu Val Ser Pro Gln Pro Gly
200 205 210
gaa gac gcc gaa ctg gtt gag cag atc cgc agg ctc cag gac gtg gac 787
Glu Asp Ala Glu Leu Val Glu Gln Ile Arg Arg Leu Gln Asp Val Asp
215 220 225
acc ctg cgg gag caa gct gca acc gca ttg gct gcg att gat ggt gcc 835
Thr Leu Arg Glu Gln Ala Ala Thr Ala Leu Ala Ala Ile Asp Gly Ala
230 235 240 245
ggc tct ctc agc gac gcc atg ggt ggt tcc ggc ggc ttt gat gaa tcc 883
Gly Ser Leu Ser Asp Ala Met Gly Gly Ser Gly Gly Phe Asp Glu Ser
250 255 260
cag gag tca gcc tct gac cag ctc ggc cag gcg gag tcc gcg ctg gca 931
Gln Glu Ser Ala Ser Asp Gln Leu Gly Gln Ala Glu Ser Ala Leu Ala
265 270 275
ggc agt gat gac tca aag ctg aaa gat att gcc gtt cag ctt gcg gaa 979
Gly Ser Asp Asp Ser Lys Leu Lys Asp Ile Ala Val Gln Leu Ala Glu
280 285 290
atc acc agc cag ctc agc caa gtg tcc atg gaa ttg ggc ggg ttc ctc 1027
Ile Thr Ser Gln Leu Ser Gln Val Ser Met Glu Leu Gly Gly Phe Leu
295 300 305
tct gat ctc ccc gca gac ccc caa gca ctc gat gac atg ctc acc cgc 1075
Ser Asp Leu Pro Ala Asp Pro Gln Ala Leu Asp Asp Met Leu Thr Arg
310 315 320 325
caa cag caa ttg aaa ctg ctc acg cgt aaa tac gct gca gat att gac 1123
Gln Gln Gln Leu Lys Leu Leu Thr Arg Lys Tyr Ala Ala Asp Ile Asp
330 335 340
ggc gtg att gag tgg cag cgg aaa gcc caa atc cgc cta gac agc att 1171
Gly Val Ile Glu Trp Gln Arg Lys Ala Gln Ile Arg Leu Asp Ser Ile
345 350 355
gac att tcc tcc gaa gcg ctt gac aag ctg aaa gaa gac gcg aaa aag 1219
Asp Ile Ser Ser Glu Ala Leu Asp Lys Leu Lys Glu Asp Ala Lys Lys
360 365 370
gcg cag gcc tcc atg atg cgt gcc gct aag aag ctt tca gct gtc cgt 1267
Ala Gln Ala Ser Met Met Arg Ala Ala Lys Lys Leu Ser Ala Val Arg
375 380 385
gca aag gca gca acc aag ttg ggg aca act gtc acc gag gag ctt cag 1315
Ala Lys Ala Ala Thr Lys Leu Gly Thr Thr Val Thr Glu Glu Leu Gln
390 395 400 405
ggc ctg gcc atg caa aaa gcc cgc ttt gag gtt gct ttg acc tcc att 1363
Gly Leu Ala Met Gln Lys Ala Arg Phe Glu Val Ala Leu Thr Ser Ile
410 415 420
gag gcg tgc gcc agc ggt atc gac cag gtg gaa ttc cag ctc gca gca 1411
Glu Ala Cys Ala Ser Gly Ile Asp Gln Val Glu Phe Gln Leu Ala Ala
425 430 435
aat gcc ttt gca cag cct cgt cca ctt gca tcc tct gcg tct ggt ggt 1459
Asn Ala Phe Ala Gln Pro Arg Pro Leu Ala Ser Ser Ala Ser Gly Gly
440 445 450
gaa ctt tcc cgc gtt atg ttg gcg ctc gag gtg atc ttg gct gct gga 1507
Glu Leu Ser Arg Val Met Leu Ala Leu Glu Val Ile Leu Ala Ala Gly
455 460 465
acc acg ggc acc acc ttg gtg ttc gac gag gtt gat gca ggt gtg ggc 1555
Thr Thr Gly Thr Thr Leu Val Phe Asp Glu Val Asp Ala Gly Val Gly
470 475 480 485
gga cgc gca gcg gtg gaa atc ggt cgc cgc ctg gcc cgc ctt gcc acc 1603
Gly Arg Ala Ala Val Glu Ile Gly Arg Arg Leu Ala Arg Leu Ala Thr
490 495 500
aaa aac caa gtc atc gtg gtc acc cat ctc cca cag gtc gct gct tac 1651
Lys Asn Gln Val Ile Val Val Thr His Leu Pro Gln Val Ala Ala Tyr
505 510 515
gcc gac acg cac ctg cac gtt gcc aag aat gta gga gaa gcc tcc gtg 1699
Ala Asp Thr His Leu His Val Ala Lys Asn Val Gly Glu Ala Ser Val
520 525 530
acc tca gga gtg gag tca ctg acc ttc gac cga cgc gtg gaa gag ctc 1747
Thr Ser Gly Val Glu Ser Leu Thr Phe Asp Arg Arg Val Glu Glu Leu
535 540 545
tcc cgc atg ctc gct ggc ctc gac gac acc gcc acc ggc cga gcc cac 1795
Ser Arg Met Leu Ala Gly Leu Asp Asp Thr Ala Thr Gly Arg Ala His
550 555 560 565
gca acg gag ctg ctc gag cgt gca cag cgt gaa aag gaa gat att aac 1843
Ala Thr Glu Leu Leu Glu Arg Ala Gln Arg Glu Lys Glu Asp Ile Asn
570 575 580
gag gag cga gta gaa cca ctt ctc gcc gcc agt gca taagagtttt 1889
Glu Glu Arg Val Glu Pro Leu Leu Ala Ala Ser Ala
585 590
cttggaattt tttaggcgcg 1909
<210> 44
<211> 593
<212> PRT
<213> Corynebacterium glutamicum
<400> 44
Met Leu Val Asp Ile Ala Ile Glu Asn Leu Gly Val Ile Pro Ala Ala
1 5 10 15
Ser Ala Glu Phe Ser Ser Gly Leu Thr Val Leu Thr Gly Glu Thr Gly
20 25 30
Ala Gly Lys Thr Met Val Val Thr Gly Leu Arg Leu Leu Ser Gly Gly
35 40 45
Arg Ala Asp Ala Ser Arg Val Arg Thr Gly Ser Pro Gln Ala Val Val
50 55 60
Glu Gly Arg Phe Val Thr Gln Gly Val Pro Cys Asp Ile Val Glu Arg
65 70 75 80
Ala Thr Gly Ile Val Ser Asn Ala Gly Gly Ala Ala Asp Glu Asn Gly
85 90 95
Glu Phe Leu Ala Val Arg Ser Val Gly Ala Asn Gly Arg Ser Lys Ala
100 105 110
His Leu Gly Gly Arg Ser Val Pro Ala Ala Thr Leu Ser Glu Phe Ser
115 120 125
Asp Glu Leu Leu Thr Ile His Gly Gln Asn Asp Gln Leu Arg Leu Leu
130 135 140
Ser Pro Glu Arg Gln Leu Glu Ala Leu Asp Arg Phe Asp Pro Glu Leu
145 150 155 160
Ala Gln Leu Arg Lys Asn Tyr Asn Ala Lys Tyr Leu Thr Trp Lys Ser
165 170 175
Leu Asp Lys Asp Leu Gln Lys Arg Leu Ser Ser Arg Arg Glu Leu Ala
180 185 190
Gln Glu Val Asp Arg Leu Gln Phe Ala Ile Asn Glu Ile Glu Glu Val
195 200 205
Ser Pro Gln Pro Gly Glu Asp Ala Glu Leu Val Glu Gln Ile Arg Arg
210 215 220
Leu Gln Asp Val Asp Thr Leu Arg Glu Gln Ala Ala Thr Ala Leu Ala
225 230 235 240
Ala Ile Asp Gly Ala Gly Ser Leu Ser Asp Ala Met Gly Gly Ser Gly
245 250 255
Gly Phe Asp Glu Ser Gln Glu Ser Ala Ser Asp Gln Leu Gly Gln Ala
260 265 270
Glu Ser Ala Leu Ala Gly Ser Asp Asp Ser Lys Leu Lys Asp Ile Ala
275 280 285
Val Gln Leu Ala Glu Ile Thr Ser Gln Leu Ser Gln Val Ser Met Glu
290 295 300
Leu Gly Gly Phe Leu Ser Asp Leu Pro Ala Asp Pro Gln Ala Leu Asp
305 310 315 320
Asp Met Leu Thr Arg Gln Gln Gln Leu Lys Leu Leu Thr Arg Lys Tyr
325 330 335
Ala Ala Asp Ile Asp Gly Val Ile Glu Trp Gln Arg Lys Ala Gln Ile
340 345 350
Arg Leu Asp Ser Ile Asp Ile Ser Ser Glu Ala Leu Asp Lys Leu Lys
355 360 365
Glu Asp Ala Lys Lys Ala Gln Ala Ser Met Met Arg Ala Ala Lys Lys
370 375 380
Leu Ser Ala Val Arg Ala Lys Ala Ala Thr Lys Leu Gly Thr Thr Val
385 390 395 400
Thr Glu Glu Leu Gln Gly Leu Ala Met Gln Lys Ala Arg Phe Glu Val
405 410 415
Ala Leu Thr Ser Ile Glu Ala Cys Ala Ser Gly Ile Asp Gln Val Glu
420 425 430
Phe Gln Leu Ala Ala Asn Ala Phe Ala Gln Pro Arg Pro Leu Ala Ser
435 440 445
Ser Ala Ser Gly Gly Glu Leu Ser Arg Val Met Leu Ala Leu Glu Val
450 455 460
Ile Leu Ala Ala Gly Thr Thr Gly Thr Thr Leu Val Phe Asp Glu Val
465 470 475 480
Asp Ala Gly Val Gly Gly Arg Ala Ala Val Glu Ile Gly Arg Arg Leu
485 490 495
Ala Arg Leu Ala Thr Lys Asn Gln Val Ile Val Val Thr His Leu Pro
500 505 510
Gln Val Ala Ala Tyr Ala Asp Thr His Leu His Val Ala Lys Asn Val
515 520 525
Gly Glu Ala Ser Val Thr Ser Gly Val Glu Ser Leu Thr Phe Asp Arg
530 535 540
Arg Val Glu Glu Leu Ser Arg Met Leu Ala Gly Leu Asp Asp Thr Ala
545 550 555 560
Thr Gly Arg Ala His Ala Thr Glu Leu Leu Glu Arg Ala Gln Arg Glu
565 570 575
Lys Glu Asp Ile Asn Glu Glu Arg Val Glu Pro Leu Leu Ala Ala Ser
580 585 590
Ala
<210> 45
<211> 265
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(235)
<223> RXA01651
<400> 45
caatctctaa ggagaaagtt tatgacaaat aggacctgac ccctgtttgg tagacaccta 60
acatcccaac attctgggac agaaaggtaa cctacctatc atg cca acc aag acc 115
Met Pro Thr Lys Thr
1 5
tac tcc gag gag ttc aaa cgc gac gcc gtt gct ttg tac gag aac tcc 163
Tyr Ser Glu Glu Phe Lys Arg Asp Ala Val Ala Leu Tyr Glu Asn Ser
10 15 20
gat ggg gcc tca ctc caa cag atc gcc aac gat ctc ggc atc aac cga 211
Asp Gly Ala Ser Leu Gln Gln Ile Ala Asn Asp Leu Gly Ile Asn Arg
25 30 35
gta acc ctg aaa aac ttc gat caa taaatacggt gcgcatgcct caaccaacac 265
Val Thr Leu Lys Asn Phe Asp Gln
40 45
<210> 46
<211> 45
<212> PRT
<213> Corynebacterium glutamicum
<400> 46
Met Pro Thr Lys Thr Tyr Ser Glu Glu Phe Lys Arg Asp Ala Val Ala
1 5 10 15
Leu Tyr Glu Asn Ser Asp Gly Ala Ser Leu Gln Gln Ile Ala Asn Asp
20 25 30
Leu Gly Ile Asn Arg Val Thr Leu Lys Asn Phe Asp Gln
35 40 45
<210> 47
<211> 538
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(508)
<223> RXA01710
<400> 47
tctcggcgct aatctggttt attggtgata tccgagccaa gggaactccg agctcaccca 60
ttaccactga tccaacgcac gaccatcttg agaggacagc atg aca gac ttc aaa 115
Met Thr Asp Phe Lys
1 5
ctc atc agc gat acc gag tgg cgc gaa cgc ctc acc ccg cag gaa ttc 163
Leu Ile Ser Asp Thr Glu Trp Arg Glu Arg Leu Thr Pro Gln Glu Phe
10 15 20
cat gtc ctc cgc gaa gcc ggc acc gaa cca cct cac gtc ggt gaa tac 211
His Val Leu Arg Glu Ala Gly Thr Glu Pro Pro His Val Gly Glu Tyr
25 30 35
acc aac acc acc acc gaa ggt gtg tac tcc tgt cgc gcc tgt ggt gaa 259
Thr Asn Thr Thr Thr Glu Gly Val Tyr Ser Cys Arg Ala Cys Gly Glu
40 45 50
gag tta ttc cgc tcc acc gag aag ttt gaa tcc cac tgc ggt tgg cct 307
Glu Leu Phe Arg Ser Thr Glu Lys Phe Glu Ser His Cys Gly Trp Pro
55 60 65
tcc ttc ttc tcc cca ctt gct ggc gac aaa atc att gag aag gaa gat 355
Ser Phe Phe Ser Pro Leu Ala Gly Asp Lys Ile Ile Glu Lys Glu Asp
70 75 80 85
ctt tcc ctc ggt atg cgt cgc gtt gag att ctg tgc gct aac tgc ggc 403
Leu Ser Leu Gly Met Arg Arg Val Glu Ile Leu Cys Ala Asn Cys Gly
90 95 100
tct cac atg ggt cac gtc ttc gaa ggc gaa ggc tac gac acc ccc acc 451
Ser His Met Gly His Val Phe Glu Gly Glu Gly Tyr Asp Thr Pro Thr
105 110 115
gat ctt cgt tac tgc att aac tcc atc agc ttg aag ctg gaa gaa aag 499
Asp Leu Arg Tyr Cys Ile Asn Ser Ile Ser Leu Lys Leu Glu Glu Lys
120 125 130
cca gtt tcc taagcttccg agcacgaaac gagccttggc 538
Pro Val Ser
135
<210> 48
<211> 136
<212> PRT
<213> Corynebacterium glutamicum
<400> 48
Met Thr Asp Phe Lys Leu Ile Ser Asp Thr Glu Trp Arg Glu Arg Leu
1 5 10 15
Thr Pro Gln Glu Phe His Val Leu Arg Glu Ala Gly Thr Glu Pro Pro
20 25 30
His Val Gly Glu Tyr Thr Asn Thr Thr Thr Glu Gly Val Tyr Ser Cys
35 40 45
Arg Ala Cys Gly Glu Glu Leu Phe Arg Ser Thr Glu Lys Phe Glu Ser
50 55 60
His Cys Gly Trp Pro Ser Phe Phe Ser Pro Leu Ala Gly Asp Lys Ile
65 70 75 80
Ile Glu Lys Glu Asp Leu Ser Leu Gly Met Arg Arg Val Glu Ile Leu
85 90 95
Cys Ala Asn Cys Gly Ser His Met Gly His Val Phe Glu Gly Glu Gly
100 105 110
Tyr Asp Thr Pro Thr Asp Leu Arg Tyr Cys Ile Asn Ser Ile Ser Leu
115 120 125
Lys Leu Glu Glu Lys Pro Val Ser
130 135
<210> 49
<211> 1417
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1387)
<223> RXA01852
<400> 49
aaccaccacc atgcgtgcag aacgcactgg taaccctttc ttgctggcac tgtagggcta 60
agttccgtac tacttcttcg aataggtatc gttaataatc gtg agt caa aac aag 115
Val Ser Gln Asn Lys
1 5
tcc aag tct gaa aag ctt cag tca ttt gct gca ccc aag ggt gtt cct 163
Ser Lys Ser Glu Lys Leu Gln Ser Phe Ala Ala Pro Lys Gly Val Pro
10 15 20
gat tac gcc cca cca aaa tct gca gcg ttt tta gca gtc cgt gat gcc 211
Asp Tyr Ala Pro Pro Lys Ser Ala Ala Phe Leu Ala Val Arg Asp Ala
25 30 35
ttt gtt aat caa gca cat aag gcc ggg ttt gag cat att gag ctg ccg 259
Phe Val Asn Gln Ala His Lys Ala Gly Phe Glu His Ile Glu Leu Pro
40 45 50
atc ttt gaa gac acc ggc ttg ttt gcg cgt ggt gtt ggt gag tcc act 307
Ile Phe Glu Asp Thr Gly Leu Phe Ala Arg Gly Val Gly Glu Ser Thr
55 60 65
gac gta gtg agc aag gaa atg tac acc ttc gct gat cgt ggc gag cgc 355
Asp Val Val Ser Lys Glu Met Tyr Thr Phe Ala Asp Arg Gly Glu Arg
70 75 80 85
tct gtc acg ctg cgc cca gaa ggc act gca ggc gtg atg cgt gca gtt 403
Ser Val Thr Leu Arg Pro Glu Gly Thr Ala Gly Val Met Arg Ala Val
90 95 100
att gaa cac agc ctg gac cgt gga cag ctt ccc gta aag ctg aac tac 451
Ile Glu His Ser Leu Asp Arg Gly Gln Leu Pro Val Lys Leu Asn Tyr
105 110 115
gcc gga cca ttc ttc cgt tat gag cgt cct cag gca ggg cgt tac cgt 499
Ala Gly Pro Phe Phe Arg Tyr Glu Arg Pro Gln Ala Gly Arg Tyr Arg
120 125 130
cag ctt cag caa gta ggc gta gag gca att ggt gtg gat gat cca gcg 547
Gln Leu Gln Gln Val Gly Val Glu Ala Ile Gly Val Asp Asp Pro Ala
135 140 145
ctt gat gcg gag atc att gcg ctt gct gat cgt tct tac cgc agc ttg 595
Leu Asp Ala Glu Ile Ile Ala Leu Ala Asp Arg Ser Tyr Arg Ser Leu
150 155 160 165
ggg ctg cag gat ttc cgt ctg gag ctc acc agc ttg ggt gat cgt cac 643
Gly Leu Gln Asp Phe Arg Leu Glu Leu Thr Ser Leu Gly Asp Arg His
170 175 180
tgc cgt ccc gag tat cgt cag aag ctg cag gat ttc ttg ttt gca ctt 691
Cys Arg Pro Glu Tyr Arg Gln Lys Leu Gln Asp Phe Leu Phe Ala Leu
185 190 195
cct ttg gat gag gaa acc cgc aag cgc gca gag atc aac cca ctt cgg 739
Pro Leu Asp Glu Glu Thr Arg Lys Arg Ala Glu Ile Asn Pro Leu Arg
200 205 210
gtg ttg gat gat aag cgt cct gaa gtc caa gag atg act gcg gat gca 787
Val Leu Asp Asp Lys Arg Pro Glu Val Gln Glu Met Thr Ala Asp Ala
215 220 225
cca ttg atg ctg gat cac ctt gat gca gag tgc cgt gag cac ttt gaa 835
Pro Leu Met Leu Asp His Leu Asp Ala Glu Cys Arg Glu His Phe Glu
230 235 240 245
aca gtg act ggt ttg ctc gat gac atg ggt gtt cca tat gtg att aac 883
Thr Val Thr Gly Leu Leu Asp Asp Met Gly Val Pro Tyr Val Ile Asn
250 255 260
cca cgc atg gtt cgt ggt ttg gat tac tac acc aag act tgt ttt gag 931
Pro Arg Met Val Arg Gly Leu Asp Tyr Tyr Thr Lys Thr Cys Phe Glu
265 270 275
ttc gtt cac gat ggc ctg ggc gca cag tct ggc att ggt ggc ggc gga 979
Phe Val His Asp Gly Leu Gly Ala Gln Ser Gly Ile Gly Gly Gly Gly
280 285 290
cgc tac gac ggt ctg atg gca cag ctt ggc gga cag gat ctg tct ggc 1027
Arg Tyr Asp Gly Leu Met Ala Gln Leu Gly Gly Gln Asp Leu Ser Gly
295 300 305
atc ggc tat ggc ctg ggt gtg gat cgc acc atg ttg gct ctg gaa gct 1075
Ile Gly Tyr Gly Leu Gly Val Asp Arg Thr Met Leu Ala Leu Glu Ala
310 315 320 325
gaa ggt gtg act gtt ggt gct gag cgt cgc gtt gat gtg tac ggc gtt 1123
Glu Gly Val Thr Val Gly Ala Glu Arg Arg Val Asp Val Tyr Gly Val
330 335 340
cca ctg ggc aag gat gct aag aag gct ctt gct gga atc gtg aac acg 1171
Pro Leu Gly Lys Asp Ala Lys Lys Ala Leu Ala Gly Ile Val Asn Thr
345 350 355
ctg cgc gct gcg ggt att tcc acc gat atg tct tac ggc gac cgt ggc 1219
Leu Arg Ala Ala Gly Ile Ser Thr Asp Met Ser Tyr Gly Asp Arg Gly
360 365 370
ctg aag ggt gcc atg aag ggc gct gac cgc tcc aac gcg ttg tac acc 1267
Leu Lys Gly Ala Met Lys Gly Ala Asp Arg Ser Asn Ala Leu Tyr Thr
375 380 385
ttg gtg ctg ggc gag cag gag ctg gag aac aac acc atc gcg gtg aag 1315
Leu Val Leu Gly Glu Gln Glu Leu Glu Asn Asn Thr Ile Ala Val Lys
390 395 400 405
gat atg cgt gcg cat gag cag cac gat gtc gca ttg gac gag gtt gtg 1363
Asp Met Arg Ala His Glu Gln His Asp Val Ala Leu Asp Glu Val Val
410 415 420
gcc ttt ttg cag ggg aaa ctt att taaataattc ataagtaaaa aaccgtcaat 1417
Ala Phe Leu Gln Gly Lys Leu Ile
425
<210> 50
<211> 429
<212> PRT
<213> Corynebacterium glutamicum
<400> 50
Val Ser Gln Asn Lys Ser Lys Ser Glu Lys Leu Gln Ser Phe Ala Ala
1 5 10 15
Pro Lys Gly Val Pro Asp Tyr Ala Pro Pro Lys Ser Ala Ala Phe Leu
20 25 30
Ala Val Arg Asp Ala Phe Val Asn Gln Ala His Lys Ala Gly Phe Glu
35 40 45
His Ile Glu Leu Pro Ile Phe Glu Asp Thr Gly Leu Phe Ala Arg Gly
50 55 60
Val Gly Glu Ser Thr Asp Val Val Ser Lys Glu Met Tyr Thr Phe Ala
65 70 75 80
Asp Arg Gly Glu Arg Ser Val Thr Leu Arg Pro Glu Gly Thr Ala Gly
85 90 95
Val Met Arg Ala Val Ile Glu His Ser Leu Asp Arg Gly Gln Leu Pro
100 105 110
Val Lys Leu Asn Tyr Ala Gly Pro Phe Phe Arg Tyr Glu Arg Pro Gln
115 120 125
Ala Gly Arg Tyr Arg Gln Leu Gln Gln Val Gly Val Glu Ala Ile Gly
130 135 140
Val Asp Asp Pro Ala Leu Asp Ala Glu Ile Ile Ala Leu Ala Asp Arg
145 150 155 160
Ser Tyr Arg Ser Leu Gly Leu Gln Asp Phe Arg Leu Glu Leu Thr Ser
165 170 175
Leu Gly Asp Arg His Cys Arg Pro Glu Tyr Arg Gln Lys Leu Gln Asp
180 185 190
Phe Leu Phe Ala Leu Pro Leu Asp Glu Glu Thr Arg Lys Arg Ala Glu
195 200 205
Ile Asn Pro Leu Arg Val Leu Asp Asp Lys Arg Pro Glu Val Gln Glu
210 215 220
Met Thr Ala Asp Ala Pro Leu Met Leu Asp His Leu Asp Ala Glu Cys
225 230 235 240
Arg Glu His Phe Glu Thr Val Thr Gly Leu Leu Asp Asp Met Gly Val
245 250 255
Pro Tyr Val Ile Asn Pro Arg Met Val Arg Gly Leu Asp Tyr Tyr Thr
260 265 270
Lys Thr Cys Phe Glu Phe Val His Asp Gly Leu Gly Ala Gln Ser Gly
275 280 285
Ile Gly Gly Gly Gly Arg Tyr Asp Gly Leu Met Ala Gln Leu Gly Gly
290 295 300
Gln Asp Leu Ser Gly Ile Gly Tyr Gly Leu Gly Val Asp Arg Thr Met
305 310 315 320
Leu Ala Leu Glu Ala Glu Gly Val Thr Val Gly Ala Glu Arg Arg Val
325 330 335
Asp Val Tyr Gly Val Pro Leu Gly Lys Asp Ala Lys Lys Ala Leu Ala
340 345 350
Gly Ile Val Asn Thr Leu Arg Ala Ala Gly Ile Ser Thr Asp Met Ser
355 360 365
Tyr Gly Asp Arg Gly Leu Lys Gly Ala Met Lys Gly Ala Asp Arg Ser
370 375 380
Asn Ala Leu Tyr Thr Leu Val Leu Gly Glu Gln Glu Leu Glu Asn Asn
385 390 395 400
Thr Ile Ala Val Lys Asp Met Arg Ala His Glu Gln His Asp Val Ala
405 410 415
Leu Asp Glu Val Val Ala Phe Leu Gln Gly Lys Leu Ile
420 425
<210> 51
<211> 955
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(925)
<223> RXA01913
<400> 51
acctgtacga tcacttttta gacgggcggg tagggctact gtgccctaac ctaagcttgt 60
aaagcattaa ttatccatac ataaggagga tcgccccgta atg gcg aac tac acc 115
Met Ala Asn Tyr Thr
1 5
gct gcg gat gtt aag aag ctc cgc gaa ctc acc ggt tcc ggc atg ctc 163
Ala Ala Asp Val Lys Lys Leu Arg Glu Leu Thr Gly Ser Gly Met Leu
10 15 20
gat tgc aag aag gct ctg gag gag tcc gct ggc gac ttc gac aag gct 211
Asp Cys Lys Lys Ala Leu Glu Glu Ser Ala Gly Asp Phe Asp Lys Ala
25 30 35
gtt gag atc ctg cgc gtc aag ggc gca aag gac gtc gga aag cgt gca 259
Val Glu Ile Leu Arg Val Lys Gly Ala Lys Asp Val Gly Lys Arg Ala
40 45 50
gag cgt aac gct acc gaa ggt ctc gtt gca gtt tct ggc aac acc atg 307
Glu Arg Asn Ala Thr Glu Gly Leu Val Ala Val Ser Gly Asn Thr Met
55 60 65
gtc gag gtc aac tct gag acc gac ttc gtt gca aag aac tct gac ttc 355
Val Glu Val Asn Ser Glu Thr Asp Phe Val Ala Lys Asn Ser Asp Phe
70 75 80 85
aag gaa ttc gct gca aag gtt gca gac gca gca gca gct gca aag gct 403
Lys Glu Phe Ala Ala Lys Val Ala Asp Ala Ala Ala Ala Ala Lys Ala
90 95 100
aac tcc cag gaa gag ctc gca gca gtt gac gtg gac gga cag acc gca 451
Asn Ser Gln Glu Glu Leu Ala Ala Val Asp Val Asp Gly Gln Thr Ala
105 110 115
gac gca gct ctg cag gag ttc tcc gca aag atc ggc gag aag ctt gag 499
Asp Ala Ala Leu Gln Glu Phe Ser Ala Lys Ile Gly Glu Lys Leu Glu
120 125 130
ctt cgt cgc gca gta acc ctc gag ggc gac aag acc gct gtt tac ctc 547
Leu Arg Arg Ala Val Thr Leu Glu Gly Asp Lys Thr Ala Val Tyr Leu
135 140 145
cac cag cgt tcc gct gac ctg cca cca gca gtt ggc gtt ttg gtt gct 595
His Gln Arg Ser Ala Asp Leu Pro Pro Ala Val Gly Val Leu Val Ala
150 155 160 165
ttc acc ggt gaa ggt gaa gca gct gag gca gct gca cgt cag gct gca 643
Phe Thr Gly Glu Gly Glu Ala Ala Glu Ala Ala Ala Arg Gln Ala Ala
170 175 180
atg cag att gct gct ctg aag gct tct tac ctc acc cgt gag gac gtt 691
Met Gln Ile Ala Ala Leu Lys Ala Ser Tyr Leu Thr Arg Glu Asp Val
185 190 195
cct gca gag atc atc gag aag gag cgc tcc atc gct gag cag atc act 739
Pro Ala Glu Ile Ile Glu Lys Glu Arg Ser Ile Ala Glu Gln Ile Thr
200 205 210
cgc gaa gag ggc aag cca gag cag gct atc cct aag atc gtt gag ggt 787
Arg Glu Glu Gly Lys Pro Glu Gln Ala Ile Pro Lys Ile Val Glu Gly
215 220 225
cgt ttg aat ggc ttc tac aag gag aac gta ctt ctt gag cag tcc tcg 835
Arg Leu Asn Gly Phe Tyr Lys Glu Asn Val Leu Leu Glu Gln Ser Ser
230 235 240 245
gta gct gac agc aag aag acc gtt aag gct ctt ctg gac gag gct ggc 883
Val Ala Asp Ser Lys Lys Thr Val Lys Ala Leu Leu Asp Glu Ala Gly
250 255 260
gtt acc gtc acc tcc ttc gct cgc ttc gag gtc ggc cag gct 925
Val Thr Val Thr Ser Phe Ala Arg Phe Glu Val Gly Gln Ala
265 270 275
taaggccact tgaaggttgt gggtgggtgt 955
<210> 52
<211> 275
<212> PRT
<213> Corynebacterium glutamicum
<400> 52
Met Ala Asn Tyr Thr Ala Ala Asp Val Lys Lys Leu Arg Glu Leu Thr
1 5 10 15
Gly Ser Gly Met Leu Asp Cys Lys Lys Ala Leu Glu Glu Ser Ala Gly
20 25 30
Asp Phe Asp Lys Ala Val Glu Ile Leu Arg Val Lys Gly Ala Lys Asp
35 40 45
Val Gly Lys Arg Ala Glu Arg Asn Ala Thr Glu Gly Leu Val Ala Val
50 55 60
Ser Gly Asn Thr Met Val Glu Val Asn Ser Glu Thr Asp Phe Val Ala
65 70 75 80
Lys Asn Ser Asp Phe Lys Glu Phe Ala Ala Lys Val Ala Asp Ala Ala
85 90 95
Ala Ala Ala Lys Ala Asn Ser Gln Glu Glu Leu Ala Ala Val Asp Val
100 105 110
Asp Gly Gln Thr Ala Asp Ala Ala Leu Gln Glu Phe Ser Ala Lys Ile
115 120 125
Gly Glu Lys Leu Glu Leu Arg Arg Ala Val Thr Leu Glu Gly Asp Lys
130 135 140
Thr Ala Val Tyr Leu His Gln Arg Ser Ala Asp Leu Pro Pro Ala Val
145 150 155 160
Gly Val Leu Val Ala Phe Thr Gly Glu Gly Glu Ala Ala Glu Ala Ala
165 170 175
Ala Arg Gln Ala Ala Met Gln Ile Ala Ala Leu Lys Ala Ser Tyr Leu
180 185 190
Thr Arg Glu Asp Val Pro Ala Glu Ile Ile Glu Lys Glu Arg Ser Ile
195 200 205
Ala Glu Gln Ile Thr Arg Glu Glu Gly Lys Pro Glu Gln Ala Ile Pro
210 215 220
Lys Ile Val Glu Gly Arg Leu Asn Gly Phe Tyr Lys Glu Asn Val Leu
225 230 235 240
Leu Glu Gln Ser Ser Val Ala Asp Ser Lys Lys Thr Val Lys Ala Leu
245 250 255
Leu Asp Glu Ala Gly Val Thr Val Thr Ser Phe Ala Arg Phe Glu Val
260 265 270
Gly Gln Ala
275
<210> 53
<211> 1747
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1717)
<223> RXA02145
<400> 53
cactgccaca gctgccaatt accgttgatg aagagggcta cctcatcgcc gctggtaact 60
tcattgagcc actcggccct gcattctggg agcgtaagtc atg agt cta gct acc 115
Met Ser Leu Ala Thr
1 5
gtg gga aac aat ctt gat tcc cgt tac acc atg gcg tcg ggt atc cgt 163
Val Gly Asn Asn Leu Asp Ser Arg Tyr Thr Met Ala Ser Gly Ile Arg
10 15 20
cgc cag atc aac aag gtc ttc cca act cac tgg tcc ttc atg ctc ggc 211
Arg Gln Ile Asn Lys Val Phe Pro Thr His Trp Ser Phe Met Leu Gly
25 30 35
gag att gcg ctt tac agc ttc atc gtc ttg ctg ctg act ggt gtc tac 259
Glu Ile Ala Leu Tyr Ser Phe Ile Val Leu Leu Leu Thr Gly Val Tyr
40 45 50
ctg acc ctg ttc ttc gac cca tca atc acc aag gtc att tat gac ggc 307
Leu Thr Leu Phe Phe Asp Pro Ser Ile Thr Lys Val Ile Tyr Asp Gly
55 60 65
ggc tac ctc cca ctg aac ggt gtg gag atg tcc cgt gca tac gca act 355
Gly Tyr Leu Pro Leu Asn Gly Val Glu Met Ser Arg Ala Tyr Ala Thr
70 75 80 85
gcg ttg gat att tcc ttc gag gtt cgc ggt ggt ctg ttc atc cgc cag 403
Ala Leu Asp Ile Ser Phe Glu Val Arg Gly Gly Leu Phe Ile Arg Gln
90 95 100
atg cac cac tgg gca gcc ctg ctg ttc gtt gta tcc atg ctg gtt cac 451
Met His His Trp Ala Ala Leu Leu Phe Val Val Ser Met Leu Val His
105 110 115
atg ctc cgt att ttc ttc acc ggt gcg ttc cgt cgc cca cgt gaa gca 499
Met Leu Arg Ile Phe Phe Thr Gly Ala Phe Arg Arg Pro Arg Glu Ala
120 125 130
aac tgg atc atc ggt gtt gtt ctg atc atc ctg ggt atg gct gaa ggc 547
Asn Trp Ile Ile Gly Val Val Leu Ile Ile Leu Gly Met Ala Glu Gly
135 140 145
ttc atg ggt tac tcc ctg cct gat gac ctg ctc tct ggt gtt ggt ctt 595
Phe Met Gly Tyr Ser Leu Pro Asp Asp Leu Leu Ser Gly Val Gly Leu
150 155 160 165
cga atc atg tcc gcc atc atc gtt ggt ctt ccg atc ata ggt acc tgg 643
Arg Ile Met Ser Ala Ile Ile Val Gly Leu Pro Ile Ile Gly Thr Trp
170 175 180
atg cac tgg ctg atc ttc ggt gga gac ttc cca tcc gat ctg atg ctg 691
Met His Trp Leu Ile Phe Gly Gly Asp Phe Pro Ser Asp Leu Met Leu
185 190 195
gac cgc ttc tac atc gca cac gtt cta atc atc cca gct atc ctg ctt 739
Asp Arg Phe Tyr Ile Ala His Val Leu Ile Ile Pro Ala Ile Leu Leu
200 205 210
ggc ttg atc gca gct cac ctg gca ctt gtt tgg tac cag aag cac acc 787
Gly Leu Ile Ala Ala His Leu Ala Leu Val Trp Tyr Gln Lys His Thr
215 220 225
cag ttc cca ggc gct ggc cgc act gag aac aac gtg atc ggt atc cga 835
Gln Phe Pro Gly Ala Gly Arg Thr Glu Asn Asn Val Ile Gly Ile Arg
230 235 240 245
atc atg cct ctg ttc gca gtt aag gct gtt gct ttc ggc ctc atc gtc 883
Ile Met Pro Leu Phe Ala Val Lys Ala Val Ala Phe Gly Leu Ile Val
250 255 260
ttc ggt ttc ctc gca ctg ctt gct ggt gtc acc acc att aac gca att 931
Phe Gly Phe Leu Ala Leu Leu Ala Gly Val Thr Thr Ile Asn Ala Ile
265 270 275
tgg aat ctt gga ccg tac aac cct tca cag gtg tct gct ggt tcc cag 979
Trp Asn Leu Gly Pro Tyr Asn Pro Ser Gln Val Ser Ala Gly Ser Gln
280 285 290
cct gac gtt tac atg ctg tgg aca gat ggt gct gct cgt gtc atg ccg 1027
Pro Asp Val Tyr Met Leu Trp Thr Asp Gly Ala Ala Arg Val Met Pro
295 300 305
gca tgg gag ctc tac ctc ggt aac tac act att cca gca gtc ttc tgg 1075
Ala Trp Glu Leu Tyr Leu Gly Asn Tyr Thr Ile Pro Ala Val Phe Trp
310 315 320 325
gtt gct gtg atg ctg ggt atc ctc gtg gtt ctg ctt gtg act tac cca 1123
Val Ala Val Met Leu Gly Ile Leu Val Val Leu Leu Val Thr Tyr Pro
330 335 340
ttc att gag cgt aag ttc acc ggc gac gat gca cac cac aac ttg ctg 1171
Phe Ile Glu Arg Lys Phe Thr Gly Asp Asp Ala His His Asn Leu Leu
345 350 355
cag cgt cct cgc gat gtt cca gtc cgc acc tca ctc ggt gtc atg gcg 1219
Gln Arg Pro Arg Asp Val Pro Val Arg Thr Ser Leu Gly Val Met Ala
360 365 370
ctt gtc ttc tac atc ctg ctt acc gtt tct ggt ggt aac gat gtt tac 1267
Leu Val Phe Tyr Ile Leu Leu Thr Val Ser Gly Gly Asn Asp Val Tyr
375 380 385
gca atg cag ttc cat gtt tca ctg aac gcg atg acc tgg atc ggt cgt 1315
Ala Met Gln Phe His Val Ser Leu Asn Ala Met Thr Trp Ile Gly Arg
390 395 400 405
atc ggc ctc atc gtt gga cca gct att gca tac ttc atc act tac cga 1363
Ile Gly Leu Ile Val Gly Pro Ala Ile Ala Tyr Phe Ile Thr Tyr Arg
410 415 420
ctg tgc atc ggc ttg cag cgc tct gac cgc gag gtc ctg gag cac ggc 1411
Leu Cys Ile Gly Leu Gln Arg Ser Asp Arg Glu Val Leu Glu His Gly
425 430 435
atc gag acc ggt atc atc aag cag atg cca aat ggt gcc ttc att gaa 1459
Ile Glu Thr Gly Ile Ile Lys Gln Met Pro Asn Gly Ala Phe Ile Glu
440 445 450
gtt cac cag cca ctt ggc cca gtt gat gac cat ggt cac cca atc cca 1507
Val His Gln Pro Leu Gly Pro Val Asp Asp His Gly His Pro Ile Pro
455 460 465
ctg cca tac gct ggc gct gcg gtt cca aag cag atg aac cag ctt ggt 1555
Leu Pro Tyr Ala Gly Ala Ala Val Pro Lys Gln Met Asn Gln Leu Gly
470 475 480 485
tac gct gag gtt gaa acc cgc ggt gga ttc ttc gga cct gat cca gaa 1603
Tyr Ala Glu Val Glu Thr Arg Gly Gly Phe Phe Gly Pro Asp Pro Glu
490 495 500
gac atc cgt gcg aag gct aag gaa att gag cac gca aac cac att gag 1651
Asp Ile Arg Ala Lys Ala Lys Glu Ile Glu His Ala Asn His Ile Glu
505 510 515
gaa gcg aac act ctt cgt gca ctc aac gag gca aac att gag cgt gac 1699
Glu Ala Asn Thr Leu Arg Ala Leu Asn Glu Ala Asn Ile Glu Arg Asp
520 525 530
aag aat gag ggc aag aac tagtttctag gacttcatct ctgaaactcc 1747
Lys Asn Glu Gly Lys Asn
535
<210> 54
<211> 539
<212> PRT
<213> Corynebacterium glutamicum
<400> 54
Met Ser Leu Ala Thr Val Gly Asn Asn Leu Asp Ser Arg Tyr Thr Met
1 5 10 15
Ala Ser Gly Ile Arg Arg Gln Ile Asn Lys Val Phe Pro Thr His Trp
20 25 30
Ser Phe Met Leu Gly Glu Ile Ala Leu Tyr Ser Phe Ile Val Leu Leu
35 40 45
Leu Thr Gly Val Tyr Leu Thr Leu Phe Phe Asp Pro Ser Ile Thr Lys
50 55 60
Val Ile Tyr Asp Gly Gly Tyr Leu Pro Leu Asn Gly Val Glu Met Ser
65 70 75 80
Arg Ala Tyr Ala Thr Ala Leu Asp Ile Ser Phe Glu Val Arg Gly Gly
85 90 95
Leu Phe Ile Arg Gln Met His His Trp Ala Ala Leu Leu Phe Val Val
100 105 110
Ser Met Leu Val His Met Leu Arg Ile Phe Phe Thr Gly Ala Phe Arg
115 120 125
Arg Pro Arg Glu Ala Asn Trp Ile Ile Gly Val Val Leu Ile Ile Leu
130 135 140
Gly Met Ala Glu Gly Phe Met Gly Tyr Ser Leu Pro Asp Asp Leu Leu
145 150 155 160
Ser Gly Val Gly Leu Arg Ile Met Ser Ala Ile Ile Val Gly Leu Pro
165 170 175
Ile Ile Gly Thr Trp Met His Trp Leu Ile Phe Gly Gly Asp Phe Pro
180 185 190
Ser Asp Leu Met Leu Asp Arg Phe Tyr Ile Ala His Val Leu Ile Ile
195 200 205
Pro Ala Ile Leu Leu Gly Leu Ile Ala Ala His Leu Ala Leu Val Trp
210 215 220
Tyr Gln Lys His Thr Gln Phe Pro Gly Ala Gly Arg Thr Glu Asn Asn
225 230 235 240
Val Ile Gly Ile Arg Ile Met Pro Leu Phe Ala Val Lys Ala Val Ala
245 250 255
Phe Gly Leu Ile Val Phe Gly Phe Leu Ala Leu Leu Ala Gly Val Thr
260 265 270
Thr Ile Asn Ala Ile Trp Asn Leu Gly Pro Tyr Asn Pro Ser Gln Val
275 280 285
Ser Ala Gly Ser Gln Pro Asp Val Tyr Met Leu Trp Thr Asp Gly Ala
290 295 300
Ala Arg Val Met Pro Ala Trp Glu Leu Tyr Leu Gly Asn Tyr Thr Ile
305 310 315 320
Pro Ala Val Phe Trp Val Ala Val Met Leu Gly Ile Leu Val Val Leu
325 330 335
Leu Val Thr Tyr Pro Phe Ile Glu Arg Lys Phe Thr Gly Asp Asp Ala
340 345 350
His His Asn Leu Leu Gln Arg Pro Arg Asp Val Pro Val Arg Thr Ser
355 360 365
Leu Gly Val Met Ala Leu Val Phe Tyr Ile Leu Leu Thr Val Ser Gly
370 375 380
Gly Asn Asp Val Tyr Ala Met Gln Phe His Val Ser Leu Asn Ala Met
385 390 395 400
Thr Trp Ile Gly Arg Ile Gly Leu Ile Val Gly Pro Ala Ile Ala Tyr
405 410 415
Phe Ile Thr Tyr Arg Leu Cys Ile Gly Leu Gln Arg Ser Asp Arg Glu
420 425 430
Val Leu Glu His Gly Ile Glu Thr Gly Ile Ile Lys Gln Met Pro Asn
435 440 445
Gly Ala Phe Ile Glu Val His Gln Pro Leu Gly Pro Val Asp Asp His
450 455 460
Gly His Pro Ile Pro Leu Pro Tyr Ala Gly Ala Ala Val Pro Lys Gln
465 470 475 480
Met Asn Gln Leu Gly Tyr Ala Glu Val Glu Thr Arg Gly Gly Phe Phe
485 490 495
Gly Pro Asp Pro Glu Asp Ile Arg Ala Lys Ala Lys Glu Ile Glu His
500 505 510
Ala Asn His Ile Glu Glu Ala Asn Thr Leu Arg Ala Leu Asn Glu Ala
515 520 525
Asn Ile Glu Arg Asp Lys Asn Glu Gly Lys Asn
530 535
<210> 55
<211> 448
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(418)
<223> RXA02236
<400> 55
gcaggctgac atccttggta ttaaccaggt gtaccctcga tttctggata ctttggtatt 60
ccttttgtca ctaaaaacca cacgataacg gaggaacccc gtg gcc ctt cca cag 115
Val Ala Leu Pro Gln
1 5
ttg act gat gag cag cgc aag gca gcg ctt gct aag gca gca gag gca 163
Leu Thr Asp Glu Gln Arg Lys Ala Ala Leu Ala Lys Ala Ala Glu Ala
10 15 20
cgc aag gca cgc gca gag ctc aaa gag aac ctg aag cgc ggc aac act 211
Arg Lys Ala Arg Ala Glu Leu Lys Glu Asn Leu Lys Arg Gly Asn Thr
25 30 35
aac ctc agg gaa gtt ctg gac aag gct gag tct gac gag atc atc ggc 259
Asn Leu Arg Glu Val Leu Asp Lys Ala Glu Ser Asp Glu Ile Ile Gly
40 45 50
aag acc aag gtc tcc gct ctc ctc gag gct ctc cct aag gtt ggc aag 307
Lys Thr Lys Val Ser Ala Leu Leu Glu Ala Leu Pro Lys Val Gly Lys
55 60 65
gtc aag gca aag gag att atg gac gag ctg ggc att gct cag acc cgt 355
Val Lys Ala Lys Glu Ile Met Asp Glu Leu Gly Ile Ala Gln Thr Arg
70 75 80 85
cgt ctt cgt gga ctg ggt gac cgt cag cgt cgc gca ctt ctc gag cgt 403
Arg Leu Arg Gly Leu Gly Asp Arg Gln Arg Arg Ala Leu Leu Glu Arg
90 95 100
ttc ggc ttc gag gat taattcttca gtgtcgggcg ataaccaact 448
Phe Gly Phe Glu Asp
105
<210> 56
<211> 106
<212> PRT
<213> Corynebacterium glutamicum
<400> 56
Val Ala Leu Pro Gln Leu Thr Asp Glu Gln Arg Lys Ala Ala Leu Ala
1 5 10 15
Lys Ala Ala Glu Ala Arg Lys Ala Arg Ala Glu Leu Lys Glu Asn Leu
20 25 30
Lys Arg Gly Asn Thr Asn Leu Arg Glu Val Leu Asp Lys Ala Glu Ser
35 40 45
Asp Glu Ile Ile Gly Lys Thr Lys Val Ser Ala Leu Leu Glu Ala Leu
50 55 60
Pro Lys Val Gly Lys Val Lys Ala Lys Glu Ile Met Asp Glu Leu Gly
65 70 75 80
Ile Ala Gln Thr Arg Arg Leu Arg Gly Leu Gly Asp Arg Gln Arg Arg
85 90 95
Ala Leu Leu Glu Arg Phe Gly Phe Glu Asp
100 105
<210> 57
<211> 1003
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(973)
<223> RXA02267
<400> 57
tgcgctcggc aagtgttttg cttatcgacg tctccccaca taacaatccc aactcgaagc 60
accaacgatt caagccttat cagttttgta caggaaaata gtg caa aaa tgg ggt 115
Val Gln Lys Trp Gly
1 5
tta agc ttc gtg gag agg att gtc atc atg aac aac gtg caa cag ttt 163
Leu Ser Phe Val Glu Arg Ile Val Ile Met Asn Asn Val Gln Gln Phe
10 15 20
cat cga ttt ttt gat gat tcc gca gtc tat tat ccc tgc ttc gtc ccg 211
His Arg Phe Phe Asp Asp Ser Ala Val Tyr Tyr Pro Cys Phe Val Pro
25 30 35
ctt gac cga gcc atc ggc gaa cac ttt gat cgt cag aac aaa ccg atg 259
Leu Asp Arg Ala Ile Gly Glu His Phe Asp Arg Gln Asn Lys Pro Met
40 45 50
tcc aga ttc atc gga acg ctc att ctg ccg tta gcc aaa ctg gaa gaa 307
Ser Arg Phe Ile Gly Thr Leu Ile Leu Pro Leu Ala Lys Leu Glu Glu
55 60 65
gcc gcc caa tac acc ggc gat gaa gtc ctt cgc gtg tcg gca gta atc 355
Ala Ala Gln Tyr Thr Gly Asp Glu Val Leu Arg Val Ser Ala Val Ile
70 75 80 85
agt act gat ggg ctc gct gat ctg cga agg gat ttt tac gaa ctc ccc 403
Ser Thr Asp Gly Leu Ala Asp Leu Arg Arg Asp Phe Tyr Glu Leu Pro
90 95 100
aac atc gac atc gcc tcg gtg gaa atc aag ctg gtc ggc gca gcc ctc 451
Asn Ile Asp Ile Ala Ser Val Glu Ile Lys Leu Val Gly Ala Ala Leu
105 110 115
acc aac acc gct tgg ttg gga gat gtg gaa aaa ctc atc caa caa cat 499
Thr Asn Thr Ala Trp Leu Gly Asp Val Glu Lys Leu Ile Gln Gln His
120 125 130
cgc aac act ttc gta tgg gtt gag att ccg aca gcc ctg gtc acc gca 547
Arg Asn Thr Phe Val Trp Val Glu Ile Pro Thr Ala Leu Val Thr Ala
135 140 145
gat att gtc cga aaa ctc cgc cac atg gga gct ggc ctg aaa tac aga 595
Asp Ile Val Arg Lys Leu Arg His Met Gly Ala Gly Leu Lys Tyr Arg
150 155 160 165
act gga ggt gat agg gaa gag ctc ttc ccc tca ccg cag gac ttg gtc 643
Thr Gly Gly Asp Arg Glu Glu Leu Phe Pro Ser Pro Gln Asp Leu Val
170 175 180
act gtg ctg cgc acc gcc atc gat gct gca ttg ccg ttt aaa ctc act 691
Thr Val Leu Arg Thr Ala Ile Asp Ala Ala Leu Pro Phe Lys Leu Thr
185 190 195
gca ggc ctg cat cgt gct ctc agg tat cgt gac gag aaa acc ggc cga 739
Ala Gly Leu His Arg Ala Leu Arg Tyr Arg Asp Glu Lys Thr Gly Arg
200 205 210
ctt cac ttc gga ttc ctc aac att gca gcc gcc gtg gcg aca ctt cgt 787
Leu His Phe Gly Phe Leu Asn Ile Ala Ala Ala Val Ala Thr Leu Arg
215 220 225
gct gga aaa ggc gag gca gag gca ctg aag atc ctt gaa ggc gat gat 835
Ala Gly Lys Gly Glu Ala Glu Ala Leu Lys Ile Leu Glu Gly Asp Asp
230 235 240 245
gcc gct ccg ctt att cac gca cta caa agc ggc gaa aac tgg cgg gat 883
Ala Ala Pro Leu Ile His Ala Leu Gln Ser Gly Glu Asn Trp Arg Asp
250 255 260
tcc ttc cgc agc ttc agt acc tgc aat gtt gtt gaa cca ctc aac act 931
Ser Phe Arg Ser Phe Ser Thr Cys Asn Val Val Glu Pro Leu Asn Thr
265 270 275
ctg att gat ctt gat gtg ttg gcg gaa gga gac gta cat ccc 973
Leu Ile Asp Leu Asp Val Leu Ala Glu Gly Asp Val His Pro
280 285 290
taaggatcga cgctagttag atcggttttt 1003
<210> 58
<211> 291
<212> PRT
<213> Corynebacterium glutamicum
<400> 58
Val Gln Lys Trp Gly Leu Ser Phe Val Glu Arg Ile Val Ile Met Asn
1 5 10 15
Asn Val Gln Gln Phe His Arg Phe Phe Asp Asp Ser Ala Val Tyr Tyr
20 25 30
Pro Cys Phe Val Pro Leu Asp Arg Ala Ile Gly Glu His Phe Asp Arg
35 40 45
Gln Asn Lys Pro Met Ser Arg Phe Ile Gly Thr Leu Ile Leu Pro Leu
50 55 60
Ala Lys Leu Glu Glu Ala Ala Gln Tyr Thr Gly Asp Glu Val Leu Arg
65 70 75 80
Val Ser Ala Val Ile Ser Thr Asp Gly Leu Ala Asp Leu Arg Arg Asp
85 90 95
Phe Tyr Glu Leu Pro Asn Ile Asp Ile Ala Ser Val Glu Ile Lys Leu
100 105 110
Val Gly Ala Ala Leu Thr Asn Thr Ala Trp Leu Gly Asp Val Glu Lys
115 120 125
Leu Ile Gln Gln His Arg Asn Thr Phe Val Trp Val Glu Ile Pro Thr
130 135 140
Ala Leu Val Thr Ala Asp Ile Val Arg Lys Leu Arg His Met Gly Ala
145 150 155 160
Gly Leu Lys Tyr Arg Thr Gly Gly Asp Arg Glu Glu Leu Phe Pro Ser
165 170 175
Pro Gln Asp Leu Val Thr Val Leu Arg Thr Ala Ile Asp Ala Ala Leu
180 185 190
Pro Phe Lys Leu Thr Ala Gly Leu His Arg Ala Leu Arg Tyr Arg Asp
195 200 205
Glu Lys Thr Gly Arg Leu His Phe Gly Phe Leu Asn Ile Ala Ala Ala
210 215 220
Val Ala Thr Leu Arg Ala Gly Lys Gly Glu Ala Glu Ala Leu Lys Ile
225 230 235 240
Leu Glu Gly Asp Asp Ala Ala Pro Leu Ile His Ala Leu Gln Ser Gly
245 250 255
Glu Asn Trp Arg Asp Ser Phe Arg Ser Phe Ser Thr Cys Asn Val Val
260 265 270
Glu Pro Leu Asn Thr Leu Ile Asp Leu Asp Val Leu Ala Glu Gly Asp
275 280 285
Val His Pro
290
<210> 59
<211> 1984
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1954)
<223> RXA02280
<400> 59
ggtcgaggtg tcgtagatgt caatgagctt cgcgattgcg tcatcgatcg ttgttgcttc 60
catgcgcacc acactatctt tctgcacgcc ctgatgccct gtg gat tca aaa ctg 115
Val Asp Ser Lys Leu
1 5
tgc ttt tat agg cgt atg caa gaa tcc tca cgt gat aat ttc caa gtt 163
Cys Phe Tyr Arg Arg Met Gln Glu Ser Ser Arg Asp Asn Phe Gln Val
10 15 20
gac ctc ggc ggc gtt gtt gat ctt ttg agt cgc cac att tat tcc ggt 211
Asp Leu Gly Gly Val Val Asp Leu Leu Ser Arg His Ile Tyr Ser Gly
25 30 35
ccg agg gtg tat gtg cgt gag ttg ctg cag aat gcg gtt gat gct tgt 259
Pro Arg Val Tyr Val Arg Glu Leu Leu Gln Asn Ala Val Asp Ala Cys
40 45 50
act gca cgt tct gaa cag ggt gag gag ggc tac gag ccg agt att cgt 307
Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly Tyr Glu Pro Ser Ile Arg
55 60 65
att cgg ccg gtg acc aag gat cgt gcc acg ttt tca ctg gtt gat aat 355
Ile Arg Pro Val Thr Lys Asp Arg Ala Thr Phe Ser Leu Val Asp Asn
70 75 80 85
ggt acg ggc ctg acc gcg cag gag gcg cgg gaa ttg ctg gcg acg gtg 403
Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg Glu Leu Leu Ala Thr Val
90 95 100
ggg cgg acg tcg aaa cgc gat gaa ttc ggt ctg cag cgg gaa ggt cgc 451
Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly Leu Gln Arg Glu Gly Arg
105 110 115
ctg ggg caa ttt ggc atc ggg ctg ctt agt tgt ttc atg gtg gcg gat 499
Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser Cys Phe Met Val Ala Asp
120 125 130
gag atc acc atg gtg tcg cat gcg gag ggt gcg tcg gcg att cgg tgg 547
Glu Ile Thr Met Val Ser His Ala Glu Gly Ala Ser Ala Ile Arg Trp
135 140 145
act ggt cat gcg gat ggc acc ttt aac ctg gag att ctt ggg gat gac 595
Thr Gly His Ala Asp Gly Thr Phe Asn Leu Glu Ile Leu Gly Asp Asp
150 155 160 165
gca acg gat gtc att ccg gtg ggc acg act gtg cac ctg act ccg cgc 643
Ala Thr Asp Val Ile Pro Val Gly Thr Thr Val His Leu Thr Pro Arg
170 175 180
cct gat gag cgc acg ttg ctg acg gaa aat tcc gtg gtc acc att gct 691
Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn Ser Val Val Thr Ile Ala
185 190 195
agt aat tat ggc cgc tac ctg ccg att cct att gtg gtg cag ggt gag 739
Ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro Ile Val Val Gln Gly Glu
200 205 210
aaa aac acc acc atc act aca tcg ccg gtg ttt gca aag gat act gat 787
Lys Asn Thr Thr Ile Thr Thr Ser Pro Val Phe Ala Lys Asp Thr Asp
215 220 225
cag cag cac agg ctg tat gcc ggc cgg gag cgc ctt ggt aaa act cct 835
Gln Gln His Arg Leu Tyr Ala Gly Arg Glu Arg Leu Gly Lys Thr Pro
230 235 240 245
ttt gat gtc atc gat ctc acc ggt cct ggc atc gag ggt gtg gct tat 883
Phe Asp Val Ile Asp Leu Thr Gly Pro Gly Ile Glu Gly Val Ala Tyr
250 255 260
gta ttg ccg gag gcc cag gct ccg cat atg tcc agg cgt cac agt att 931
Val Leu Pro Glu Ala Gln Ala Pro His Met Ser Arg Arg His Ser Ile
265 270 275
tat gtc aac cgc atg ttg gtc tct gat ggg cct tcc acg gtg ctg ccc 979
Tyr Val Asn Arg Met Leu Val Ser Asp Gly Pro Ser Thr Val Leu Pro
280 285 290
aac tgg gcg ttc ttt gtg gaa tgt gaa atc aat tca acc gat ttg gaa 1027
Asn Trp Ala Phe Phe Val Glu Cys Glu Ile Asn Ser Thr Asp Leu Glu
295 300 305
ccc acc gca tcg cgt gaa gcg ctc atg gat gac acc gcg ttc gcg gca 1075
Pro Thr Ala Ser Arg Glu Ala Leu Met Asp Asp Thr Ala Phe Ala Ala
310 315 320 325
acc agg gaa cat atc ggt gag tgc att aaa tcg tgg ctg att aat ctc 1123
Thr Arg Glu His Ile Gly Glu Cys Ile Lys Ser Trp Leu Ile Asn Leu
330 335 340
gcc atg acc aag cct cac cgc gtg cgg gaa ttt act gcg att cat gat 1171
Ala Met Thr Lys Pro His Arg Val Arg Glu Phe Thr Ala Ile His Asp
345 350 355
ctt gcc ctg cgc gag ctg tgc caa tcg gac gcg gac ctg gct gaa acc 1219
Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp Ala Asp Leu Ala Glu Thr
360 365 370
atg ttg ggt ctt ctc acc ttg gag acc tcc cgt ggt cgc atc tcg atc 1267
Met Leu Gly Leu Leu Thr Leu Glu Thr Ser Arg Gly Arg Ile Ser Ile
375 380 385
ggt gag atc acc acg ttg tcc atc acc gag gat gtg tcg ctg cag ctg 1315
Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu Asp Val Ser Leu Gln Leu
390 395 400 405
gct acc acg ttg gat gat ttc agg cag ctc aac acc att gcg cgc ccg 1363
Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu Asn Thr Ile Ala Arg Pro
410 415 420
gac acc ttg att att aat ggc ggc tac att cac gac agc gat ctg gct 1411
Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile His Asp Ser Asp Leu Ala
425 430 435
cgg ctc att ccc gtt cac tac cca ccg ctt acg gta tct act gct gac 1459
Arg Leu Ile Pro Val His Tyr Pro Pro Leu Thr Val Ser Thr Ala Asp
440 445 450
ctg cgc gaa tcc atg gat ctg atg gag ctt ccg ccg ctg cag gac att 1507
Leu Arg Glu Ser Met Asp Leu Met Glu Leu Pro Pro Leu Gln Asp Ile
455 460 465
gag aaa gcc aag gca ctg gat gcg cag gtc acg gaa tca ttg aag gat 1555
Glu Lys Ala Lys Ala Leu Asp Ala Gln Val Thr Glu Ser Leu Lys Asp
470 475 480 485
ttt cag atc aag ggc gca acg agg gtt ttt gaa ccc gca gat gtt cct 1603
Phe Gln Ile Lys Gly Ala Thr Arg Val Phe Glu Pro Ala Asp Val Pro
490 495 500
gcc gtg gtg atc att gat tcc aag gcg cag gcc tca cgg gat cgc aat 1651
Ala Val Val Ile Ile Asp Ser Lys Ala Gln Ala Ser Arg Asp Arg Asn
505 510 515
gaa aca caa agc gca acc act gat cgt tgg gct gac att ttg gca acg 1699
Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp Ala Asp Ile Leu Ala Thr
520 525 530
gtg gat aac acg ttg agc cgt caa aca gcc aac att cca cag gat cag 1747
Val Asp Asn Thr Leu Ser Arg Gln Thr Ala Asn Ile Pro Gln Asp Gln
535 540 545
gga ctg tcg gcg ttg tgc ttg aat tgg aac aat tcg ctg gtc agg aaa 1795
Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn Asn Ser Leu Val Arg Lys
550 555 560 565
ttg gcg tcc act gat gac acc gcc gtg gtg tcg cgc acg gtg cgt ttg 1843
Leu Ala Ser Thr Asp Asp Thr Ala Val Val Ser Arg Thr Val Arg Leu
570 575 580
ctc tac gtt cag gca ttg ttg tcc agc aag agg cca ctg cgg gtg aag 1891
Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys Arg Pro Leu Arg Val Lys
585 590 595
gaa cgc gcg ctg ctt aat gat tcg ctg gca gat ctg gtt tct ttg tct 1939
Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala Asp Leu Val Ser Leu Ser
600 605 610
ttg tca tcc gat atc taagacaatc ctccgctaat ctcgagggca 1984
Leu Ser Ser Asp Ile
615
<210> 60
<211> 618
<212> PRT
<213> Corynebacterium glutamicum
<400> 60
Val Asp Ser Lys Leu Cys Phe Tyr Arg Arg Met Gln Glu Ser Ser Arg
1 5 10 15
Asp Asn Phe Gln Val Asp Leu Gly Gly Val Val Asp Leu Leu Ser Arg
20 25 30
His Ile Tyr Ser Gly Pro Arg Val Tyr Val Arg Glu Leu Leu Gln Asn
35 40 45
Ala Val Asp Ala Cys Thr Ala Arg Ser Glu Gln Gly Glu Glu Gly Tyr
50 55 60
Glu Pro Ser Ile Arg Ile Arg Pro Val Thr Lys Asp Arg Ala Thr Phe
65 70 75 80
Ser Leu Val Asp Asn Gly Thr Gly Leu Thr Ala Gln Glu Ala Arg Glu
85 90 95
Leu Leu Ala Thr Val Gly Arg Thr Ser Lys Arg Asp Glu Phe Gly Leu
100 105 110
Gln Arg Glu Gly Arg Leu Gly Gln Phe Gly Ile Gly Leu Leu Ser Cys
115 120 125
Phe Met Val Ala Asp Glu Ile Thr Met Val Ser His Ala Glu Gly Ala
130 135 140
Ser Ala Ile Arg Trp Thr Gly His Ala Asp Gly Thr Phe Asn Leu Glu
145 150 155 160
Ile Leu Gly Asp Asp Ala Thr Asp Val Ile Pro Val Gly Thr Thr Val
165 170 175
His Leu Thr Pro Arg Pro Asp Glu Arg Thr Leu Leu Thr Glu Asn Ser
180 185 190
Val Val Thr Ile Ala Ser Asn Tyr Gly Arg Tyr Leu Pro Ile Pro Ile
195 200 205
Val Val Gln Gly Glu Lys Asn Thr Thr Ile Thr Thr Ser Pro Val Phe
210 215 220
Ala Lys Asp Thr Asp Gln Gln His Arg Leu Tyr Ala Gly Arg Glu Arg
225 230 235 240
Leu Gly Lys Thr Pro Phe Asp Val Ile Asp Leu Thr Gly Pro Gly Ile
245 250 255
Glu Gly Val Ala Tyr Val Leu Pro Glu Ala Gln Ala Pro His Met Ser
260 265 270
Arg Arg His Ser Ile Tyr Val Asn Arg Met Leu Val Ser Asp Gly Pro
275 280 285
Ser Thr Val Leu Pro Asn Trp Ala Phe Phe Val Glu Cys Glu Ile Asn
290 295 300
Ser Thr Asp Leu Glu Pro Thr Ala Ser Arg Glu Ala Leu Met Asp Asp
305 310 315 320
Thr Ala Phe Ala Ala Thr Arg Glu His Ile Gly Glu Cys Ile Lys Ser
325 330 335
Trp Leu Ile Asn Leu Ala Met Thr Lys Pro His Arg Val Arg Glu Phe
340 345 350
Thr Ala Ile His Asp Leu Ala Leu Arg Glu Leu Cys Gln Ser Asp Ala
355 360 365
Asp Leu Ala Glu Thr Met Leu Gly Leu Leu Thr Leu Glu Thr Ser Arg
370 375 380
Gly Arg Ile Ser Ile Gly Glu Ile Thr Thr Leu Ser Ile Thr Glu Asp
385 390 395 400
Val Ser Leu Gln Leu Ala Thr Thr Leu Asp Asp Phe Arg Gln Leu Asn
405 410 415
Thr Ile Ala Arg Pro Asp Thr Leu Ile Ile Asn Gly Gly Tyr Ile His
420 425 430
Asp Ser Asp Leu Ala Arg Leu Ile Pro Val His Tyr Pro Pro Leu Thr
435 440 445
Val Ser Thr Ala Asp Leu Arg Glu Ser Met Asp Leu Met Glu Leu Pro
450 455 460
Pro Leu Gln Asp Ile Glu Lys Ala Lys Ala Leu Asp Ala Gln Val Thr
465 470 475 480
Glu Ser Leu Lys Asp Phe Gln Ile Lys Gly Ala Thr Arg Val Phe Glu
485 490 495
Pro Ala Asp Val Pro Ala Val Val Ile Ile Asp Ser Lys Ala Gln Ala
500 505 510
Ser Arg Asp Arg Asn Glu Thr Gln Ser Ala Thr Thr Asp Arg Trp Ala
515 520 525
Asp Ile Leu Ala Thr Val Asp Asn Thr Leu Ser Arg Gln Thr Ala Asn
530 535 540
Ile Pro Gln Asp Gln Gly Leu Ser Ala Leu Cys Leu Asn Trp Asn Asn
545 550 555 560
Ser Leu Val Arg Lys Leu Ala Ser Thr Asp Asp Thr Ala Val Val Ser
565 570 575
Arg Thr Val Arg Leu Leu Tyr Val Gln Ala Leu Leu Ser Ser Lys Arg
580 585 590
Pro Leu Arg Val Lys Glu Arg Ala Leu Leu Asn Asp Ser Leu Ala Asp
595 600 605
Leu Val Ser Leu Ser Leu Ser Ser Asp Ile
610 615
<210> 61
<211> 1792
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1762)
<223> RXA02388
<400> 61
ttcgggagag caacggtggg tttagcaccg tggaggattt actgcaggtc aaggggattg 60
ggccctcaaa gtttgagcag atctctggat tggtgtcccc atg att gag gtg cgt 115
Met Ile Glu Val Arg
1 5
ttg gtt ccg gtg gcg gct gtg atg tgg atg gct gtc gct gcg ttg att 163
Leu Val Pro Val Ala Ala Val Met Trp Met Ala Val Ala Ala Leu Ile
10 15 20
atc agt ggt tcg tgg gtg ttg tcg gtg ggg att gtt ggc atc gcg atc 211
Ile Ser Gly Ser Trp Val Leu Ser Val Gly Ile Val Gly Ile Ala Ile
25 30 35
att gct gct tgt gtg ttt aaa cac tgg ggt caa gct gtg gtg ata gct 259
Ile Ala Ala Cys Val Phe Lys His Trp Gly Gln Ala Val Val Ile Ala
40 45 50
gca ctg ggc gtt ggt gcc gta gtg atg gct gcg ttg aga atc agc agc 307
Ala Leu Gly Val Gly Ala Val Val Met Ala Ala Leu Arg Ile Ser Ser
55 60 65
gcg aag gca ttt gaa gca ccg caa acc tgg gtg ggt acc gca gaa acc 355
Ala Lys Ala Phe Glu Ala Pro Gln Thr Trp Val Gly Thr Ala Glu Thr
70 75 80 85
atc aag ttt tta gac agc ggt gat caa cta atc ggt ttg aga gta gaa 403
Ile Lys Phe Leu Asp Ser Gly Asp Gln Leu Ile Gly Leu Arg Val Glu
90 95 100
ggc tat cca gcg ccg att cca gtg ttt tac tct ggt agc gac acc att 451
Gly Tyr Pro Ala Pro Ile Pro Val Phe Tyr Ser Gly Ser Asp Thr Ile
105 110 115
gag aaa gcc tct ctc att gca gtg tcc ggt cgg att aaa cca gat agt 499
Glu Lys Ala Ser Leu Ile Ala Val Ser Gly Arg Ile Lys Pro Asp Ser
120 125 130
ttc cct ggg gtg ggt gat ctg acc att tcc act gaa gac att gat cag 547
Phe Pro Gly Val Gly Asp Leu Thr Ile Ser Thr Glu Asp Ile Asp Gln
135 140 145
ttg gaa ccg acc act ggt tat agc gca tgg gtg aac cag gtg cgt gac 595
Leu Glu Pro Thr Thr Gly Tyr Ser Ala Trp Val Asn Gln Val Arg Asp
150 155 160 165
ggg ttt tcc caa gcc gtg gaa gaa acc gtg ggg gag tct tcc cgt gga 643
Gly Phe Ser Gln Ala Val Glu Glu Thr Val Gly Glu Ser Ser Arg Gly
170 175 180
ctg att cca ggc atg gtg ttg ggg gat acg cgg ttg cag ggg tca att 691
Leu Ile Pro Gly Met Val Leu Gly Asp Thr Arg Leu Gln Gly Ser Ile
185 190 195
gaa gcc caa acc tat att gat acg ggg ttg tct cac ctg tca gct gtt 739
Glu Ala Gln Thr Tyr Ile Asp Thr Gly Leu Ser His Leu Ser Ala Val
200 205 210
agt gga agc aat gta gcc att gtg gtg tcc tct gtg gtg gtg ttg tcg 787
Ser Gly Ser Asn Val Ala Ile Val Val Ser Ser Val Val Val Leu Ser
215 220 225
tat ttt ctc acc gct ggg cca cgc atc agg gtg gtg gcg tca ttg ctg 835
Tyr Phe Leu Thr Ala Gly Pro Arg Ile Arg Val Val Ala Ser Leu Leu
230 235 240 245
tcc tta gtt att ttt gtc tcc ctc gtg ggg ttt gaa cca agt gtg ctt 883
Ser Leu Val Ile Phe Val Ser Leu Val Gly Phe Glu Pro Ser Val Leu
250 255 260
cgt gct tcg gtc aca ggc atc gtg ggg ctt ctg gca atc atc aac tct 931
Arg Ala Ser Val Thr Gly Ile Val Gly Leu Leu Ala Ile Ile Asn Ser
265 270 275
tct cgg atg gag ccg atg cat ggg ttg agt ctt tcg gtg att tgc tta 979
Ser Arg Met Glu Pro Met His Gly Leu Ser Leu Ser Val Ile Cys Leu
280 285 290
ctg ttt tat gat tcc aac ctg gcg gtg cat tac gga ttc tta ctc tcg 1027
Leu Phe Tyr Asp Ser Asn Leu Ala Val His Tyr Gly Phe Leu Leu Ser
295 300 305
tgt gca gca act gct ggc att gtg atg ctt caa cca ctg ctg tac cgt 1075
Cys Ala Ala Thr Ala Gly Ile Val Met Leu Gln Pro Leu Leu Tyr Arg
310 315 320 325
gcc atc ggt cca cca ctg gcg gtg tgg aaa gta cca gac atc gtg gtg 1123
Ala Ile Gly Pro Pro Leu Ala Val Trp Lys Val Pro Asp Ile Val Val
330 335 340
cgc gct ttc gcg gtg tcc att gcc gct gat ctg gtg acc atc ccg att 1171
Arg Ala Phe Ala Val Ser Ile Ala Ala Asp Leu Val Thr Ile Pro Ile
345 350 355
atc gct ctg atg gct cgc caa ata tcc ctc gtg gca gtg ctg gcc aac 1219
Ile Ala Leu Met Ala Arg Gln Ile Ser Leu Val Ala Val Leu Ala Asn
360 365 370
gtg ttg gtt gaa tta gct gtt cca ccc atc acg ttg ctt ggg ttg att 1267
Val Leu Val Glu Leu Ala Val Pro Pro Ile Thr Leu Leu Gly Leu Ile
375 380 385
gcc gtg ctg gca agc ctt ctt ccc tgg cca gtg gaa tac cca ctc ttg 1315
Ala Val Leu Ala Ser Leu Leu Pro Trp Pro Val Glu Tyr Pro Leu Leu
390 395 400 405
aaa atc att gag ccc ttc acc tgg tgg att cat cac gtg gcc aag tgg 1363
Lys Ile Ile Glu Pro Phe Thr Trp Trp Ile His His Val Ala Lys Trp
410 415 420
tgc caa caa tta ccc aat tcg acg ctg gaa ata agt gct ggt tgg gca 1411
Cys Gln Gln Leu Pro Asn Ser Thr Leu Glu Ile Ser Ala Gly Trp Ala
425 430 435
ggg att gcc tgg gcg tgt atg gca gcg gtg tgg gtg gtg gtg att atc 1459
Gly Ile Ala Trp Ala Cys Met Ala Ala Val Trp Val Val Val Ile Ile
440 445 450
tac aaa gga tat gtg cgc acc ctt gca gtg tgt tgt gtc tgc ttc ttt 1507
Tyr Lys Gly Tyr Val Arg Thr Leu Ala Val Cys Cys Val Cys Phe Phe
455 460 465
ctt ttc ggc gcg tgg aat aac aga ctg cca gcc caa ata gat ccg aca 1555
Leu Phe Gly Ala Trp Asn Asn Arg Leu Pro Ala Gln Ile Asp Pro Thr
470 475 480 485
gag ctg cgg ttt gtc atc atc gcc gat gat tct gag ctc act gat gtg 1603
Glu Leu Arg Phe Val Ile Ile Ala Asp Asp Ser Glu Leu Thr Asp Val
490 495 500
ccc gaa cat gca gaa ttg atc atc gtg gaa gac ccc cac ggc agc atg 1651
Pro Glu His Ala Glu Leu Ile Ile Val Glu Asp Pro His Gly Ser Met
505 510 515
tcc gat cgc ccc atc gtc acc aga gaa gga atc cct gtg ctg tat cca 1699
Ser Asp Arg Pro Ile Val Thr Arg Glu Gly Ile Pro Val Leu Tyr Pro
520 525 530
tac cgc gat ggg gag gtc agc ctt cat att gat ggc acc cag cat gca 1747
Tyr Arg Asp Gly Glu Val Ser Leu His Ile Asp Gly Thr Gln His Ala
535 540 545
gcg gac ggg aga ttt taacgacact tgtggcacga tggtcacgtg 1792
Ala Asp Gly Arg Phe
550
<210> 62
<211> 554
<212> PRT
<213> Corynebacterium glutamicum
<400> 62
Met Ile Glu Val Arg Leu Val Pro Val Ala Ala Val Met Trp Met Ala
1 5 10 15
Val Ala Ala Leu Ile Ile Ser Gly Ser Trp Val Leu Ser Val Gly Ile
20 25 30
Val Gly Ile Ala Ile Ile Ala Ala Cys Val Phe Lys His Trp Gly Gln
35 40 45
Ala Val Val Ile Ala Ala Leu Gly Val Gly Ala Val Val Met Ala Ala
50 55 60
Leu Arg Ile Ser Ser Ala Lys Ala Phe Glu Ala Pro Gln Thr Trp Val
65 70 75 80
Gly Thr Ala Glu Thr Ile Lys Phe Leu Asp Ser Gly Asp Gln Leu Ile
85 90 95
Gly Leu Arg Val Glu Gly Tyr Pro Ala Pro Ile Pro Val Phe Tyr Ser
100 105 110
Gly Ser Asp Thr Ile Glu Lys Ala Ser Leu Ile Ala Val Ser Gly Arg
115 120 125
Ile Lys Pro Asp Ser Phe Pro Gly Val Gly Asp Leu Thr Ile Ser Thr
130 135 140
Glu Asp Ile Asp Gln Leu Glu Pro Thr Thr Gly Tyr Ser Ala Trp Val
145 150 155 160
Asn Gln Val Arg Asp Gly Phe Ser Gln Ala Val Glu Glu Thr Val Gly
165 170 175
Glu Ser Ser Arg Gly Leu Ile Pro Gly Met Val Leu Gly Asp Thr Arg
180 185 190
Leu Gln Gly Ser Ile Glu Ala Gln Thr Tyr Ile Asp Thr Gly Leu Ser
195 200 205
His Leu Ser Ala Val Ser Gly Ser Asn Val Ala Ile Val Val Ser Ser
210 215 220
Val Val Val Leu Ser Tyr Phe Leu Thr Ala Gly Pro Arg Ile Arg Val
225 230 235 240
Val Ala Ser Leu Leu Ser Leu Val Ile Phe Val Ser Leu Val Gly Phe
245 250 255
Glu Pro Ser Val Leu Arg Ala Ser Val Thr Gly Ile Val Gly Leu Leu
260 265 270
Ala Ile Ile Asn Ser Ser Arg Met Glu Pro Met His Gly Leu Ser Leu
275 280 285
Ser Val Ile Cys Leu Leu Phe Tyr Asp Ser Asn Leu Ala Val His Tyr
290 295 300
Gly Phe Leu Leu Ser Cys Ala Ala Thr Ala Gly Ile Val Met Leu Gln
305 310 315 320
Pro Leu Leu Tyr Arg Ala Ile Gly Pro Pro Leu Ala Val Trp Lys Val
325 330 335
Pro Asp Ile Val Val Arg Ala Phe Ala Val Ser Ile Ala Ala Asp Leu
340 345 350
Val Thr Ile Pro Ile Ile Ala Leu Met Ala Arg Gln Ile Ser Leu Val
355 360 365
Ala Val Leu Ala Asn Val Leu Val Glu Leu Ala Val Pro Pro Ile Thr
370 375 380
Leu Leu Gly Leu Ile Ala Val Leu Ala Ser Leu Leu Pro Trp Pro Val
385 390 395 400
Glu Tyr Pro Leu Leu Lys Ile Ile Glu Pro Phe Thr Trp Trp Ile His
405 410 415
His Val Ala Lys Trp Cys Gln Gln Leu Pro Asn Ser Thr Leu Glu Ile
420 425 430
Ser Ala Gly Trp Ala Gly Ile Ala Trp Ala Cys Met Ala Ala Val Trp
435 440 445
Val Val Val Ile Ile Tyr Lys Gly Tyr Val Arg Thr Leu Ala Val Cys
450 455 460
Cys Val Cys Phe Phe Leu Phe Gly Ala Trp Asn Asn Arg Leu Pro Ala
465 470 475 480
Gln Ile Asp Pro Thr Glu Leu Arg Phe Val Ile Ile Ala Asp Asp Ser
485 490 495
Glu Leu Thr Asp Val Pro Glu His Ala Glu Leu Ile Ile Val Glu Asp
500 505 510
Pro His Gly Ser Met Ser Asp Arg Pro Ile Val Thr Arg Glu Gly Ile
515 520 525
Pro Val Leu Tyr Pro Tyr Arg Asp Gly Glu Val Ser Leu His Ile Asp
530 535 540
Gly Thr Gln His Ala Ala Asp Gly Arg Phe
545 550
<210> 63
<211> 2977
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2947)
<223> RXA02416
<400> 63
agtcatgtgg ttcagactag cggaaacacc ttgttcgatg ctatgttcga aggtgtattt 60
tttgaagcga caaaaatcga ttttgaaggg cagttgagca ttg gct gat cgc ctc 115
Leu Ala Asp Arg Leu
1 5
gta gtg cgc gga gcg cgt gaa cat aac cta aaa ggc gtg gat att gat 163
Val Val Arg Gly Ala Arg Glu His Asn Leu Lys Gly Val Asp Ile Asp
10 15 20
ttg cca cgc gac tcg atg gtg gtg ttc acc ggc ctg tca ggt tcc ggt 211
Leu Pro Arg Asp Ser Met Val Val Phe Thr Gly Leu Ser Gly Ser Gly
25 30 35
aaa tca tca ctg gcc ttt gac acc atc ttt gcg gaa ggc cag cgc cgt 259
Lys Ser Ser Leu Ala Phe Asp Thr Ile Phe Ala Glu Gly Gln Arg Arg
40 45 50
tac gtg gag tcg ttg tcc agt tac gcc cgc atg ttc ttg ggg cag atg 307
Tyr Val Glu Ser Leu Ser Ser Tyr Ala Arg Met Phe Leu Gly Gln Met
55 60 65
gac aag ccg gac gtg gat ttg att gat gga tta tcc cca gcg gtc tcc 355
Asp Lys Pro Asp Val Asp Leu Ile Asp Gly Leu Ser Pro Ala Val Ser
70 75 80 85
att gac caa aaa tcc acc aac cgc aac cct cgg tcc aca gtc ggt acc 403
Ile Asp Gln Lys Ser Thr Asn Arg Asn Pro Arg Ser Thr Val Gly Thr
90 95 100
atc acg gaa gtc tat gac tac ctg cgt ctt ctg tac gcc cgc gct ggt 451
Ile Thr Glu Val Tyr Asp Tyr Leu Arg Leu Leu Tyr Ala Arg Ala Gly
105 110 115
acc gca cac tgc cca gtg tgt gat gcc cgc gtg gag cgt caa acc ccc 499
Thr Ala His Cys Pro Val Cys Asp Ala Arg Val Glu Arg Gln Thr Pro
120 125 130
cag cag atg gtg gac caa atc ctt ggc atg gag gag gga ctg aag ttc 547
Gln Gln Met Val Asp Gln Ile Leu Gly Met Glu Glu Gly Leu Lys Phe
135 140 145
caa atc ctt gcg cct gtg gtg cgt acc cgt aaa ggt gag ttc gtt gat 595
Gln Ile Leu Ala Pro Val Val Arg Thr Arg Lys Gly Glu Phe Val Asp
150 155 160 165
ctt ttc gca gat ctt gca tcc caa ggt tat tcc cgc gtg cgg gtt gat 643
Leu Phe Ala Asp Leu Ala Ser Gln Gly Tyr Ser Arg Val Arg Val Asp
170 175 180
ggg gaa gtg cac cag ctc tcg gat cct cca aag cta gaa aag cag atc 691
Gly Glu Val His Gln Leu Ser Asp Pro Pro Lys Leu Glu Lys Gln Ile
185 190 195
aag cac gat att gat gtt gtg gtt gac cgt ctg cag gta aaa gcc agc 739
Lys His Asp Ile Asp Val Val Val Asp Arg Leu Gln Val Lys Ala Ser
200 205 210
caa aag cag cgc ctg aca gac tct atg gaa acc gca ctt cgc ctg gcc 787
Gln Lys Gln Arg Leu Thr Asp Ser Met Glu Thr Ala Leu Arg Leu Ala
215 220 225
gat ggc gtg gct gtg ctg gag ttc gtt ggc ctg gag gaa gat gat ccg 835
Asp Gly Val Ala Val Leu Glu Phe Val Gly Leu Glu Glu Asp Asp Pro
230 235 240 245
aat agg ctt cgt cga ttc tct gaa aag atg agc tgc cct aac ggt cac 883
Asn Arg Leu Arg Arg Phe Ser Glu Lys Met Ser Cys Pro Asn Gly His
250 255 260
gcg ttg acg gtt gat gag ctg gag cct cgt gct ttt tcc ttc aac tct 931
Ala Leu Thr Val Asp Glu Leu Glu Pro Arg Ala Phe Ser Phe Asn Ser
265 270 275
cct tat ggc gcg tgt cct gcc tgt gat ggc ttg ggt gtg cgc acc gaa 979
Pro Tyr Gly Ala Cys Pro Ala Cys Asp Gly Leu Gly Val Arg Thr Glu
280 285 290
gtt gat att gat ctg atc atc cca gat cca gat gca cct gca act aaa 1027
Val Asp Ile Asp Leu Ile Ile Pro Asp Pro Asp Ala Pro Ala Thr Lys
295 300 305
gcg gtt cag ccc tgg aac tcc agc cca aac cac tct tac ttt gaa aag 1075
Ala Val Gln Pro Trp Asn Ser Ser Pro Asn His Ser Tyr Phe Glu Lys
310 315 320 325
ctc att gaa ggc ctg gcg aaa gcc ctc gga ttt gat ccg gaa act ccg 1123
Leu Ile Glu Gly Leu Ala Lys Ala Leu Gly Phe Asp Pro Glu Thr Pro
330 335 340
tac agt gag ctc acc gca gct caa aag aag gct ctg gtc tat gga tcg 1171
Tyr Ser Glu Leu Thr Ala Ala Gln Lys Lys Ala Leu Val Tyr Gly Ser
345 350 355
aag gaa gaa gta agc gtt cga tac aag aac cgc tac gga cgc gtg cgt 1219
Lys Glu Glu Val Ser Val Arg Tyr Lys Asn Arg Tyr Gly Arg Val Arg
360 365 370
tct tgg act gcg cct ttt gaa ggt gtc atg ggc tac ttt gat cgc aag 1267
Ser Trp Thr Ala Pro Phe Glu Gly Val Met Gly Tyr Phe Asp Arg Lys
375 380 385
ttg gag cag act gat tcc gaa acc caa aaa gac cga ctg ttg ggc tac 1315
Leu Glu Gln Thr Asp Ser Glu Thr Gln Lys Asp Arg Leu Leu Gly Tyr
390 395 400 405
acc cgt gaa gtg ccc tgc cca acc tgt aaa ggc gca cgc ctc aag ccg 1363
Thr Arg Glu Val Pro Cys Pro Thr Cys Lys Gly Ala Arg Leu Lys Pro
410 415 420
gaa atc ttg gcc gtt cgc cta gac tcc gga agc cat gga gcg ttg tcc 1411
Glu Ile Leu Ala Val Arg Leu Asp Ser Gly Ser His Gly Ala Leu Ser
425 430 435
att gct gga cta acc gcg ctg tcg gtg cat gaa gca ttc gag ttt ttg 1459
Ile Ala Gly Leu Thr Ala Leu Ser Val His Glu Ala Phe Glu Phe Leu
440 445 450
gat aac ctc aca ctg ggc aag cgc gag gaa atg atc gcg gga gct gtg 1507
Asp Asn Leu Thr Leu Gly Lys Arg Glu Glu Met Ile Ala Gly Ala Val
455 460 465
ctg aag gaa att cac gcc cgc ctg aaa ttc ctg ctt gac gtg ggc ctt 1555
Leu Lys Glu Ile His Ala Arg Leu Lys Phe Leu Leu Asp Val Gly Leu
470 475 480 485
tcc tac ctc acc ctt gat cgc gcc gca ggc acc ctg tct ggt ggt gaa 1603
Ser Tyr Leu Thr Leu Asp Arg Ala Ala Gly Thr Leu Ser Gly Gly Glu
490 495 500
gcg cag cgt atc cgc ctg gct act caa att ggt tcc ggt ctg gct ggt 1651
Ala Gln Arg Ile Arg Leu Ala Thr Gln Ile Gly Ser Gly Leu Ala Gly
505 510 515
gtg ctc tac gtc ttg gat gag cca tcc att ggt ctg cac caa cgt gac 1699
Val Leu Tyr Val Leu Asp Glu Pro Ser Ile Gly Leu His Gln Arg Asp
520 525 530
aac cag cgc ttg atc act acc ctt gag cat ctc cga gat atc gga aac 1747
Asn Gln Arg Leu Ile Thr Thr Leu Glu His Leu Arg Asp Ile Gly Asn
535 540 545
acg ctc att gtt gtg gaa cac gat gaa gac acc atc agg cgc gca gat 1795
Thr Leu Ile Val Val Glu His Asp Glu Asp Thr Ile Arg Arg Ala Asp
550 555 560 565
tgg ctc gtg gat att ggt cct cga gct ggt gaa ttt ggt ggc gaa gtg 1843
Trp Leu Val Asp Ile Gly Pro Arg Ala Gly Glu Phe Gly Gly Glu Val
570 575 580
gtc tac caa ggt gag ccg aag ggc att ttg gac tgc gaa gaa tcc ctc 1891
Val Tyr Gln Gly Glu Pro Lys Gly Ile Leu Asp Cys Glu Glu Ser Leu
585 590 595
aca ggt gct tac ttg tct ggt cgt cga acc ctg ggt gtt cct gat act 1939
Thr Gly Ala Tyr Leu Ser Gly Arg Arg Thr Leu Gly Val Pro Asp Thr
600 605 610
cgc cgt gag atc gac aaa gag cga cag ctc aag gtg gtt ggt gct agg 1987
Arg Arg Glu Ile Asp Lys Glu Arg Gln Leu Lys Val Val Gly Ala Arg
615 620 625
gaa aat aac ctg cag ggc atc gat gtg aaa atc cca ctg ggt gtg ctg 2035
Glu Asn Asn Leu Gln Gly Ile Asp Val Lys Ile Pro Leu Gly Val Leu
630 635 640 645
tgc tgc atc act ggt gtg tcg gga tct ggt aaa tcc acg ctg gtc aat 2083
Cys Cys Ile Thr Gly Val Ser Gly Ser Gly Lys Ser Thr Leu Val Asn
650 655 660
cag att ttg gcc aag gtt ctg gcc aac aaa ctc aac cgc gca cgc caa 2131
Gln Ile Leu Ala Lys Val Leu Ala Asn Lys Leu Asn Arg Ala Arg Gln
665 670 675
gtg cct ggt cgc gca aag cgg gtg gaa ggc ctc gag cac ttg gat aag 2179
Val Pro Gly Arg Ala Lys Arg Val Glu Gly Leu Glu His Leu Asp Lys
680 685 690
ttg gtc caa gtg gat cag tcg cca att ggt cgt act cca cgt tca aac 2227
Leu Val Gln Val Asp Gln Ser Pro Ile Gly Arg Thr Pro Arg Ser Asn
695 700 705
cca gcg acg tac acg ggt gtg ttt gat aaa gtc cgt aac ctt ttt gcc 2275
Pro Ala Thr Tyr Thr Gly Val Phe Asp Lys Val Arg Asn Leu Phe Ala
710 715 720 725
gag acc act gaa gcg aag gtc cgc ggt tac aag cct ggc cgc ttc tcc 2323
Glu Thr Thr Glu Ala Lys Val Arg Gly Tyr Lys Pro Gly Arg Phe Ser
730 735 740
ttc aat att aag ggt gga cgc tgc gaa gca tgt cag ggc gat ggc acg 2371
Phe Asn Ile Lys Gly Gly Arg Cys Glu Ala Cys Gln Gly Asp Gly Thr
745 750 755
ctg aag atc gaa atg aac ttc ctg ccc gac gtg tat gtt ccg tgt gaa 2419
Leu Lys Ile Glu Met Asn Phe Leu Pro Asp Val Tyr Val Pro Cys Glu
760 765 770
gtc tgt gat ggt cag cgc tac aac cgc gag acc ctc gag gtg aag tac 2467
Val Cys Asp Gly Gln Arg Tyr Asn Arg Glu Thr Leu Glu Val Lys Tyr
775 780 785
aag ggc aaa aac atc gct gaa gta ttg ggc atg ccg atc tct gag gct 2515
Lys Gly Lys Asn Ile Ala Glu Val Leu Gly Met Pro Ile Ser Glu Ala
790 795 800 805
gcg gac ttc ttt gag ccc atc acc tca att cac cga tac cta gca acg 2563
Ala Asp Phe Phe Glu Pro Ile Thr Ser Ile His Arg Tyr Leu Ala Thr
810 815 820
ctg gtt gat gtc ggc ctt ggc tat gtc cgt ttg ggc cag gca gca aca 2611
Leu Val Asp Val Gly Leu Gly Tyr Val Arg Leu Gly Gln Ala Ala Thr
825 830 835
acc ttg tct ggt ggt gaa gcc cag cgt gtg aaa ctt gcc gct gag ctg 2659
Thr Leu Ser Gly Gly Glu Ala Gln Arg Val Lys Leu Ala Ala Glu Leu
840 845 850
cag aag cgt tcc aac ggt cgc acc gtt tac atc ctc gat gag cca act 2707
Gln Lys Arg Ser Asn Gly Arg Thr Val Tyr Ile Leu Asp Glu Pro Thr
855 860 865
act ggt ttg cac ttt gaa gat att cgc aaa ctc atg atg gtg atc gaa 2755
Thr Gly Leu His Phe Glu Asp Ile Arg Lys Leu Met Met Val Ile Glu
870 875 880 885
ggc ctg gtg gac aag ggt aac tcc gtg atc atc atc gag cac aac ctc 2803
Gly Leu Val Asp Lys Gly Asn Ser Val Ile Ile Ile Glu His Asn Leu
890 895 900
gac gtg atc aag gct gcc gac tgg atc gtg gac atg ggt cca gag ggc 2851
Asp Val Ile Lys Ala Ala Asp Trp Ile Val Asp Met Gly Pro Glu Gly
905 910 915
gga agc ggc ggt gga acc gtg gtc gct gaa gga acc cca gag caa gtt 2899
Gly Ser Gly Gly Gly Thr Val Val Ala Glu Gly Thr Pro Glu Gln Val
920 925 930
gct gaa gtt gcg ggt tcc tac acc ggc caa ttc ctt aaa gag ttg ttg 2947
Ala Glu Val Ala Gly Ser Tyr Thr Gly Gln Phe Leu Lys Glu Leu Leu
935 940 945
taggagaaga tgaggggctt tcatgggaag 2977
<210> 64
<211> 949
<212> PRT
<213> Corynebacterium glutamicum
<400> 64
Leu Ala Asp Arg Leu Val Val Arg Gly Ala Arg Glu His Asn Leu Lys
1 5 10 15
Gly Val Asp Ile Asp Leu Pro Arg Asp Ser Met Val Val Phe Thr Gly
20 25 30
Leu Ser Gly Ser Gly Lys Ser Ser Leu Ala Phe Asp Thr Ile Phe Ala
35 40 45
Glu Gly Gln Arg Arg Tyr Val Glu Ser Leu Ser Ser Tyr Ala Arg Met
50 55 60
Phe Leu Gly Gln Met Asp Lys Pro Asp Val Asp Leu Ile Asp Gly Leu
65 70 75 80
Ser Pro Ala Val Ser Ile Asp Gln Lys Ser Thr Asn Arg Asn Pro Arg
85 90 95
Ser Thr Val Gly Thr Ile Thr Glu Val Tyr Asp Tyr Leu Arg Leu Leu
100 105 110
Tyr Ala Arg Ala Gly Thr Ala His Cys Pro Val Cys Asp Ala Arg Val
115 120 125
Glu Arg Gln Thr Pro Gln Gln Met Val Asp Gln Ile Leu Gly Met Glu
130 135 140
Glu Gly Leu Lys Phe Gln Ile Leu Ala Pro Val Val Arg Thr Arg Lys
145 150 155 160
Gly Glu Phe Val Asp Leu Phe Ala Asp Leu Ala Ser Gln Gly Tyr Ser
165 170 175
Arg Val Arg Val Asp Gly Glu Val His Gln Leu Ser Asp Pro Pro Lys
180 185 190
Leu Glu Lys Gln Ile Lys His Asp Ile Asp Val Val Val Asp Arg Leu
195 200 205
Gln Val Lys Ala Ser Gln Lys Gln Arg Leu Thr Asp Ser Met Glu Thr
210 215 220
Ala Leu Arg Leu Ala Asp Gly Val Ala Val Leu Glu Phe Val Gly Leu
225 230 235 240
Glu Glu Asp Asp Pro Asn Arg Leu Arg Arg Phe Ser Glu Lys Met Ser
245 250 255
Cys Pro Asn Gly His Ala Leu Thr Val Asp Glu Leu Glu Pro Arg Ala
260 265 270
Phe Ser Phe Asn Ser Pro Tyr Gly Ala Cys Pro Ala Cys Asp Gly Leu
275 280 285
Gly Val Arg Thr Glu Val Asp Ile Asp Leu Ile Ile Pro Asp Pro Asp
290 295 300
Ala Pro Ala Thr Lys Ala Val Gln Pro Trp Asn Ser Ser Pro Asn His
305 310 315 320
Ser Tyr Phe Glu Lys Leu Ile Glu Gly Leu Ala Lys Ala Leu Gly Phe
325 330 335
Asp Pro Glu Thr Pro Tyr Ser Glu Leu Thr Ala Ala Gln Lys Lys Ala
340 345 350
Leu Val Tyr Gly Ser Lys Glu Glu Val Ser Val Arg Tyr Lys Asn Arg
355 360 365
Tyr Gly Arg Val Arg Ser Trp Thr Ala Pro Phe Glu Gly Val Met Gly
370 375 380
Tyr Phe Asp Arg Lys Leu Glu Gln Thr Asp Ser Glu Thr Gln Lys Asp
385 390 395 400
Arg Leu Leu Gly Tyr Thr Arg Glu Val Pro Cys Pro Thr Cys Lys Gly
405 410 415
Ala Arg Leu Lys Pro Glu Ile Leu Ala Val Arg Leu Asp Ser Gly Ser
420 425 430
His Gly Ala Leu Ser Ile Ala Gly Leu Thr Ala Leu Ser Val His Glu
435 440 445
Ala Phe Glu Phe Leu Asp Asn Leu Thr Leu Gly Lys Arg Glu Glu Met
450 455 460
Ile Ala Gly Ala Val Leu Lys Glu Ile His Ala Arg Leu Lys Phe Leu
465 470 475 480
Leu Asp Val Gly Leu Ser Tyr Leu Thr Leu Asp Arg Ala Ala Gly Thr
485 490 495
Leu Ser Gly Gly Glu Ala Gln Arg Ile Arg Leu Ala Thr Gln Ile Gly
500 505 510
Ser Gly Leu Ala Gly Val Leu Tyr Val Leu Asp Glu Pro Ser Ile Gly
515 520 525
Leu His Gln Arg Asp Asn Gln Arg Leu Ile Thr Thr Leu Glu His Leu
530 535 540
Arg Asp Ile Gly Asn Thr Leu Ile Val Val Glu His Asp Glu Asp Thr
545 550 555 560
Ile Arg Arg Ala Asp Trp Leu Val Asp Ile Gly Pro Arg Ala Gly Glu
565 570 575
Phe Gly Gly Glu Val Val Tyr Gln Gly Glu Pro Lys Gly Ile Leu Asp
580 585 590
Cys Glu Glu Ser Leu Thr Gly Ala Tyr Leu Ser Gly Arg Arg Thr Leu
595 600 605
Gly Val Pro Asp Thr Arg Arg Glu Ile Asp Lys Glu Arg Gln Leu Lys
610 615 620
Val Val Gly Ala Arg Glu Asn Asn Leu Gln Gly Ile Asp Val Lys Ile
625 630 635 640
Pro Leu Gly Val Leu Cys Cys Ile Thr Gly Val Ser Gly Ser Gly Lys
645 650 655
Ser Thr Leu Val Asn Gln Ile Leu Ala Lys Val Leu Ala Asn Lys Leu
660 665 670
Asn Arg Ala Arg Gln Val Pro Gly Arg Ala Lys Arg Val Glu Gly Leu
675 680 685
Glu His Leu Asp Lys Leu Val Gln Val Asp Gln Ser Pro Ile Gly Arg
690 695 700
Thr Pro Arg Ser Asn Pro Ala Thr Tyr Thr Gly Val Phe Asp Lys Val
705 710 715 720
Arg Asn Leu Phe Ala Glu Thr Thr Glu Ala Lys Val Arg Gly Tyr Lys
725 730 735
Pro Gly Arg Phe Ser Phe Asn Ile Lys Gly Gly Arg Cys Glu Ala Cys
740 745 750
Gln Gly Asp Gly Thr Leu Lys Ile Glu Met Asn Phe Leu Pro Asp Val
755 760 765
Tyr Val Pro Cys Glu Val Cys Asp Gly Gln Arg Tyr Asn Arg Glu Thr
770 775 780
Leu Glu Val Lys Tyr Lys Gly Lys Asn Ile Ala Glu Val Leu Gly Met
785 790 795 800
Pro Ile Ser Glu Ala Ala Asp Phe Phe Glu Pro Ile Thr Ser Ile His
805 810 815
Arg Tyr Leu Ala Thr Leu Val Asp Val Gly Leu Gly Tyr Val Arg Leu
820 825 830
Gly Gln Ala Ala Thr Thr Leu Ser Gly Gly Glu Ala Gln Arg Val Lys
835 840 845
Leu Ala Ala Glu Leu Gln Lys Arg Ser Asn Gly Arg Thr Val Tyr Ile
850 855 860
Leu Asp Glu Pro Thr Thr Gly Leu His Phe Glu Asp Ile Arg Lys Leu
865 870 875 880
Met Met Val Ile Glu Gly Leu Val Asp Lys Gly Asn Ser Val Ile Ile
885 890 895
Ile Glu His Asn Leu Asp Val Ile Lys Ala Ala Asp Trp Ile Val Asp
900 905 910
Met Gly Pro Glu Gly Gly Ser Gly Gly Gly Thr Val Val Ala Glu Gly
915 920 925
Thr Pro Glu Gln Val Ala Glu Val Ala Gly Ser Tyr Thr Gly Gln Phe
930 935 940
Leu Lys Glu Leu Leu
945
<210> 65
<211> 697
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(667)
<223> RXA02418
<400> 65
gacacggact agtgcccgtg tataactctt gaaagtcaga tctcccgttc atcctagatg 60
atgaacgggt ttttggtctg cataggggca atcacctcaa gtg gtt cgg tac gtc 115
Val Val Arg Tyr Val
1 5
aaa ttt tcc cgc act gct aac aga gga gtc cac atc agc gct gaa gct 163
Lys Phe Ser Arg Thr Ala Asn Arg Gly Val His Ile Ser Ala Glu Ala
10 15 20
cgc att aat gag cgc atc cga gtt ccc gaa gtc cgc ctt gtc gga cct 211
Arg Ile Asn Glu Arg Ile Arg Val Pro Glu Val Arg Leu Val Gly Pro
25 30 35
aac ggt gag caa gta ggc atc gtc cgt atc gaa gat gcc cgc aag ctc 259
Asn Gly Glu Gln Val Gly Ile Val Arg Ile Glu Asp Ala Arg Lys Leu
40 45 50
gca ttc gac gca gac cta gac ctg gtc gag gtc gca ccc aac gcc aaa 307
Ala Phe Asp Ala Asp Leu Asp Leu Val Glu Val Ala Pro Asn Ala Lys
55 60 65
cct cca gtc tgc aag atc atg gac tac gga aag ttc aag tac gaa gcg 355
Pro Pro Val Cys Lys Ile Met Asp Tyr Gly Lys Phe Lys Tyr Glu Ala
70 75 80 85
gcc caa aag gct cgt gag tca cgc aag aat cag cag cag acc gtg gtc 403
Ala Gln Lys Ala Arg Glu Ser Arg Lys Asn Gln Gln Gln Thr Val Val
90 95 100
aaa gag caa aag ctt cgt ccc aag atc gat gat cat gat tat gag acg 451
Lys Glu Gln Lys Leu Arg Pro Lys Ile Asp Asp His Asp Tyr Glu Thr
105 110 115
aag aag aac aat gtg atc cga ttc ctt gaa aag gga tca aag gtc aaa 499
Lys Lys Asn Asn Val Ile Arg Phe Leu Glu Lys Gly Ser Lys Val Lys
120 125 130
gtc acg atc atg ttc cgt ggt cgt gag cag gct cgc cca gag ctt ggc 547
Val Thr Ile Met Phe Arg Gly Arg Glu Gln Ala Arg Pro Glu Leu Gly
135 140 145
tac agg ctc ctc gag cga ctg gca aac gat gtc gta gat ttt ggc atc 595
Tyr Arg Leu Leu Glu Arg Leu Ala Asn Asp Val Val Asp Phe Gly Ile
150 155 160 165
gtg gaa acc cgc gca aag cag gac gga cga aac atg aca atg gtt ctc 643
Val Glu Thr Arg Ala Lys Gln Asp Gly Arg Asn Met Thr Met Val Leu
170 175 180
ggt ccg gtg cgc aag ggc aag aaa taatcacgaa tagggtttaa ggacaacttt 697
Gly Pro Val Arg Lys Gly Lys Lys
185
<210> 66
<211> 189
<212> PRT
<213> Corynebacterium glutamicum
<400> 66
Val Val Arg Tyr Val Lys Phe Ser Arg Thr Ala Asn Arg Gly Val His
1 5 10 15
Ile Ser Ala Glu Ala Arg Ile Asn Glu Arg Ile Arg Val Pro Glu Val
20 25 30
Arg Leu Val Gly Pro Asn Gly Glu Gln Val Gly Ile Val Arg Ile Glu
35 40 45
Asp Ala Arg Lys Leu Ala Phe Asp Ala Asp Leu Asp Leu Val Glu Val
50 55 60
Ala Pro Asn Ala Lys Pro Pro Val Cys Lys Ile Met Asp Tyr Gly Lys
65 70 75 80
Phe Lys Tyr Glu Ala Ala Gln Lys Ala Arg Glu Ser Arg Lys Asn Gln
85 90 95
Gln Gln Thr Val Val Lys Glu Gln Lys Leu Arg Pro Lys Ile Asp Asp
100 105 110
His Asp Tyr Glu Thr Lys Lys Asn Asn Val Ile Arg Phe Leu Glu Lys
115 120 125
Gly Ser Lys Val Lys Val Thr Ile Met Phe Arg Gly Arg Glu Gln Ala
130 135 140
Arg Pro Glu Leu Gly Tyr Arg Leu Leu Glu Arg Leu Ala Asn Asp Val
145 150 155 160
Val Asp Phe Gly Ile Val Glu Thr Arg Ala Lys Gln Asp Gly Arg Asn
165 170 175
Met Thr Met Val Leu Gly Pro Val Arg Lys Gly Lys Lys
180 185
<210> 67
<211> 2419
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2389)
<223> RXA02429
<400> 67
tcttacccac cagtgcaatg taggtcacgt cgtatcacgt ctgagggtga ttgagtaggg 60
ttaaacagat gaattcattt agctcaccgg aggtataacc gtg gcc ggt ttt gat 115
Val Ala Gly Phe Asp
1 5
tgg ttt tgg aag gcc ctt ggc ggc aaa tcg ggc aga aac caa aaa cgt 163
Trp Phe Trp Lys Ala Leu Gly Gly Lys Ser Gly Arg Asn Gln Lys Arg
10 15 20
agc gtg gca att gtc aat cag gta gaa aac cat gca gcg gaa tta gac 211
Ser Val Ala Ile Val Asn Gln Val Glu Asn His Ala Ala Glu Leu Asp
25 30 35
gcg ctg gat gat gtt gca ttg gcg cag cgt gcc aag gat cta gcc agt 259
Ala Leu Asp Asp Val Ala Leu Ala Gln Arg Ala Lys Asp Leu Ala Ser
40 45 50
ggt gga cgc att gac aat cat gcg gaa ttc ctc gcc att ttg ggt gtg 307
Gly Gly Arg Ile Asp Asn His Ala Glu Phe Leu Ala Ile Leu Gly Val
55 60 65
gca tcg cag cgg aca ttg ggg ctg aag ccg tat ccg gtg caa tca cag 355
Ala Ser Gln Arg Thr Leu Gly Leu Lys Pro Tyr Pro Val Gln Ser Gln
70 75 80 85
gcg gtg ttg cgt ctc att gaa ggc gat gtg gtg cac atg gct acc ggt 403
Ala Val Leu Arg Leu Ile Glu Gly Asp Val Val His Met Ala Thr Gly
90 95 100
gag ggc aag act ttg gtg ggc gcg atg gcg gcc acc ggt ctg ggg ttg 451
Glu Gly Lys Thr Leu Val Gly Ala Met Ala Ala Thr Gly Leu Gly Leu
105 110 115
atg ggc aag cga gtc cat tcg att acc gtc aat gat tat ttg gcg gtg 499
Met Gly Lys Arg Val His Ser Ile Thr Val Asn Asp Tyr Leu Ala Val
120 125 130
cgc gat gcc gaa tgg atg cgg cca ttg gtc gaa ttt ttc ggt ctg agc 547
Arg Asp Ala Glu Trp Met Arg Pro Leu Val Glu Phe Phe Gly Leu Ser
135 140 145
gtg gcg agc atc agc gag aag atg gat gca ggg gag cgt cga caa gca 595
Val Ala Ser Ile Ser Glu Lys Met Asp Ala Gly Glu Arg Arg Gln Ala
150 155 160 165
tat aaa gcc gca att gtc tac gga cct gtc aat gaa atc ggc ttt gac 643
Tyr Lys Ala Ala Ile Val Tyr Gly Pro Val Asn Glu Ile Gly Phe Asp
170 175 180
gtg ctg cgt gat cag cta att acc cgg cgc gaa gac gcc gtg cag cat 691
Val Leu Arg Asp Gln Leu Ile Thr Arg Arg Glu Asp Ala Val Gln His
185 190 195
ggc gcc gac gtc gcg att atc gat gag gcc gat tcc gtg ctt gtc gac 739
Gly Ala Asp Val Ala Ile Ile Asp Glu Ala Asp Ser Val Leu Val Asp
200 205 210
gag gcc ctg gtg cca ctc gtc ctc gcc ggc aac cag ccc ggc cat gcg 787
Glu Ala Leu Val Pro Leu Val Leu Ala Gly Asn Gln Pro Gly His Ala
215 220 225
ccg cgc ggc aaa atc acc gat gtg gtg cgc tcg ttg aaa gaa aac gac 835
Pro Arg Gly Lys Ile Thr Asp Val Val Arg Ser Leu Lys Glu Asn Asp
230 235 240 245
gat tac acc atc gac gat gat cgt cgc aac gtc ttc ctc acc gac aag 883
Asp Tyr Thr Ile Asp Asp Asp Arg Arg Asn Val Phe Leu Thr Asp Lys
250 255 260
ggt gcc gcc aaa tta gag cag cag ctg ggc atc agc agc ctc tac gac 931
Gly Ala Ala Lys Leu Glu Gln Gln Leu Gly Ile Ser Ser Leu Tyr Asp
265 270 275
gat gag cac gtc ggc tcg acg ctc gtg cag gtc aac ctc gcc ctc cac 979
Asp Glu His Val Gly Ser Thr Leu Val Gln Val Asn Leu Ala Leu His
280 285 290
gcg cag gca ctg ctc atc cgc gac atc cac tac atc gtc cgc gac agc 1027
Ala Gln Ala Leu Leu Ile Arg Asp Ile His Tyr Ile Val Arg Asp Ser
295 300 305
aag gtc ttg ctt atc gac gcc tcc cgc ggc cgt gtc gcc gac ctg cag 1075
Lys Val Leu Leu Ile Asp Ala Ser Arg Gly Arg Val Ala Asp Leu Gln
310 315 320 325
cgc tgg ccc gac ggc ctg caa gca gca gtg gag gcc aag gaa ggt ctc 1123
Arg Trp Pro Asp Gly Leu Gln Ala Ala Val Glu Ala Lys Glu Gly Leu
330 335 340
gcg gtt tct gaa ggc ggc aag atc ctt gac acc atc aca ctt cag gcg 1171
Ala Val Ser Glu Gly Gly Lys Ile Leu Asp Thr Ile Thr Leu Gln Ala
345 350 355
ttg att ggt cgc tac cca atg gca tgc ggc atg aca ggt acc gcc gtg 1219
Leu Ile Gly Arg Tyr Pro Met Ala Cys Gly Met Thr Gly Thr Ala Val
360 365 370
gag gca acc gat cag cta cgc acc ttc tat gac ttg cat gtt tct gtc 1267
Glu Ala Thr Asp Gln Leu Arg Thr Phe Tyr Asp Leu His Val Ser Val
375 380 385
att gag cgc aat cat ccg ctg aag cgc ttt gat gaa gct gac cgt atc 1315
Ile Glu Arg Asn His Pro Leu Lys Arg Phe Asp Glu Ala Asp Arg Ile
390 395 400 405
tac gcc acc atg gcg gag aaa aac cgc gcc atc atc gat gaa atc gca 1363
Tyr Ala Thr Met Ala Glu Lys Asn Arg Ala Ile Ile Asp Glu Ile Ala
410 415 420
ctc ctt cac agc acg ggg cag cca gtc ctg gtg ggt acc cac gat gtg 1411
Leu Leu His Ser Thr Gly Gln Pro Val Leu Val Gly Thr His Asp Val
425 430 435
gca gag tcg gaa gaa ctc gcc act gca ctg cgt gaa ctc aac atc gaa 1459
Ala Glu Ser Glu Glu Leu Ala Thr Ala Leu Arg Glu Leu Asn Ile Glu
440 445 450
gta agc gtt ctc aac gcc aag aat gat gcc gaa gaa gcc cag atc atc 1507
Val Ser Val Leu Asn Ala Lys Asn Asp Ala Glu Glu Ala Gln Ile Ile
455 460 465
gca gag gct ggc gat att gga cga gtg acc gtt tcc act cag atg gcc 1555
Ala Glu Ala Gly Asp Ile Gly Arg Val Thr Val Ser Thr Gln Met Ala
470 475 480 485
ggc cgc ggt acc gat att cgc ctc ggt ggc gcc gat gaa gcc gac tac 1603
Gly Arg Gly Thr Asp Ile Arg Leu Gly Gly Ala Asp Glu Ala Asp Tyr
490 495 500
gat gaa gtg gtg aaa ctc ggt gga ctc gcc gtt atc ggc acc gcc cgc 1651
Asp Glu Val Val Lys Leu Gly Gly Leu Ala Val Ile Gly Thr Ala Arg
505 510 515
cac cgt tct cag cgc ctg gac aac cag ctg cgc gga cgt gcg gga cga 1699
His Arg Ser Gln Arg Leu Asp Asn Gln Leu Arg Gly Arg Ala Gly Arg
520 525 530
caa gga gat cca ggc ctg agc ctt ttc ttt gtc tcc ctc gat gat gat 1747
Gln Gly Asp Pro Gly Leu Ser Leu Phe Phe Val Ser Leu Asp Asp Asp
535 540 545
gtg gtg gtc tca ggc ggg tca agg gag agc gtg agc gcg caa ccc gat 1795
Val Val Val Ser Gly Gly Ser Arg Glu Ser Val Ser Ala Gln Pro Asp
550 555 560 565
gcc acc ggg ctg att gac tca gat cgc atc cgc gat tgg gtc gga cac 1843
Ala Thr Gly Leu Ile Asp Ser Asp Arg Ile Arg Asp Trp Val Gly His
570 575 580
tgc cag cgc gtc acc gaa gga cag ctg ctg gaa atc cac tcc cag agc 1891
Cys Gln Arg Val Thr Glu Gly Gln Leu Leu Glu Ile His Ser Gln Ser
585 590 595
tgg aat tac aac aag ctc ctt gcc gat caa cgc gtg atc att gac gag 1939
Trp Asn Tyr Asn Lys Leu Leu Ala Asp Gln Arg Val Ile Ile Asp Glu
600 605 610
cgc cgc gaa cgc ctc ctc gac acc gcc tta gcg tgg gag gaa ctg gca 1987
Arg Arg Glu Arg Leu Leu Asp Thr Ala Leu Ala Trp Glu Glu Leu Ala
615 620 625
cag cat gca cca gcg cgg gct gca gag ctt gaa gac ctt gat cag tcc 2035
Gln His Ala Pro Ala Arg Ala Ala Glu Leu Glu Asp Leu Asp Gln Ser
630 635 640 645
gtg agg gaa cag gca gca cga gac atc atg ctg tac cac ctc gat tac 2083
Val Arg Glu Gln Ala Ala Arg Asp Ile Met Leu Tyr His Leu Asp Tyr
650 655 660
aac tgg tca gag cac ctc gcg ttg atg gat gat gtc cgc gaa tcc att 2131
Asn Trp Ser Glu His Leu Ala Leu Met Asp Asp Val Arg Glu Ser Ile
665 670 675
cac ctg cgc gcc atc gcc agg gaa acc ccc ctt gat gaa tac cac cgc 2179
His Leu Arg Ala Ile Ala Arg Glu Thr Pro Leu Asp Glu Tyr His Arg
680 685 690
atc gct gtg cgt gaa ttc aag gat ttg gca caa cgc gct gtc gat gat 2227
Ile Ala Val Arg Glu Phe Lys Asp Leu Ala Gln Arg Ala Val Asp Asp
695 700 705
gcg gtg tcc acg ttc aag tct gtg acc atc gat cac gag ggt gcc cat 2275
Ala Val Ser Thr Phe Lys Ser Val Thr Ile Asp His Glu Gly Ala His
710 715 720 725
ttg gat gat gag ggc ttg gcg cgt cca tca gca acg tgg acc tac atg 2323
Leu Asp Asp Glu Gly Leu Ala Arg Pro Ser Ala Thr Trp Thr Tyr Met
730 735 740
gtc tct gac aac cca ctt gcg ggt agt ggt aac tca gtg atc agt ggc 2371
Val Ser Asp Asn Pro Leu Ala Gly Ser Gly Asn Ser Val Ile Ser Gly
745 750 755
ata gga aat atc ttt aga taacctgaga actatgaaat tccagctcac 2419
Ile Gly Asn Ile Phe Arg
760
<210> 68
<211> 763
<212> PRT
<213> Corynebacterium glutamicum
<400> 68
Val Ala Gly Phe Asp Trp Phe Trp Lys Ala Leu Gly Gly Lys Ser Gly
1 5 10 15
Arg Asn Gln Lys Arg Ser Val Ala Ile Val Asn Gln Val Glu Asn His
20 25 30
Ala Ala Glu Leu Asp Ala Leu Asp Asp Val Ala Leu Ala Gln Arg Ala
35 40 45
Lys Asp Leu Ala Ser Gly Gly Arg Ile Asp Asn His Ala Glu Phe Leu
50 55 60
Ala Ile Leu Gly Val Ala Ser Gln Arg Thr Leu Gly Leu Lys Pro Tyr
65 70 75 80
Pro Val Gln Ser Gln Ala Val Leu Arg Leu Ile Glu Gly Asp Val Val
85 90 95
His Met Ala Thr Gly Glu Gly Lys Thr Leu Val Gly Ala Met Ala Ala
100 105 110
Thr Gly Leu Gly Leu Met Gly Lys Arg Val His Ser Ile Thr Val Asn
115 120 125
Asp Tyr Leu Ala Val Arg Asp Ala Glu Trp Met Arg Pro Leu Val Glu
130 135 140
Phe Phe Gly Leu Ser Val Ala Ser Ile Ser Glu Lys Met Asp Ala Gly
145 150 155 160
Glu Arg Arg Gln Ala Tyr Lys Ala Ala Ile Val Tyr Gly Pro Val Asn
165 170 175
Glu Ile Gly Phe Asp Val Leu Arg Asp Gln Leu Ile Thr Arg Arg Glu
180 185 190
Asp Ala Val Gln His Gly Ala Asp Val Ala Ile Ile Asp Glu Ala Asp
195 200 205
Ser Val Leu Val Asp Glu Ala Leu Val Pro Leu Val Leu Ala Gly Asn
210 215 220
Gln Pro Gly His Ala Pro Arg Gly Lys Ile Thr Asp Val Val Arg Ser
225 230 235 240
Leu Lys Glu Asn Asp Asp Tyr Thr Ile Asp Asp Asp Arg Arg Asn Val
245 250 255
Phe Leu Thr Asp Lys Gly Ala Ala Lys Leu Glu Gln Gln Leu Gly Ile
260 265 270
Ser Ser Leu Tyr Asp Asp Glu His Val Gly Ser Thr Leu Val Gln Val
275 280 285
Asn Leu Ala Leu His Ala Gln Ala Leu Leu Ile Arg Asp Ile His Tyr
290 295 300
Ile Val Arg Asp Ser Lys Val Leu Leu Ile Asp Ala Ser Arg Gly Arg
305 310 315 320
Val Ala Asp Leu Gln Arg Trp Pro Asp Gly Leu Gln Ala Ala Val Glu
325 330 335
Ala Lys Glu Gly Leu Ala Val Ser Glu Gly Gly Lys Ile Leu Asp Thr
340 345 350
Ile Thr Leu Gln Ala Leu Ile Gly Arg Tyr Pro Met Ala Cys Gly Met
355 360 365
Thr Gly Thr Ala Val Glu Ala Thr Asp Gln Leu Arg Thr Phe Tyr Asp
370 375 380
Leu His Val Ser Val Ile Glu Arg Asn His Pro Leu Lys Arg Phe Asp
385 390 395 400
Glu Ala Asp Arg Ile Tyr Ala Thr Met Ala Glu Lys Asn Arg Ala Ile
405 410 415
Ile Asp Glu Ile Ala Leu Leu His Ser Thr Gly Gln Pro Val Leu Val
420 425 430
Gly Thr His Asp Val Ala Glu Ser Glu Glu Leu Ala Thr Ala Leu Arg
435 440 445
Glu Leu Asn Ile Glu Val Ser Val Leu Asn Ala Lys Asn Asp Ala Glu
450 455 460
Glu Ala Gln Ile Ile Ala Glu Ala Gly Asp Ile Gly Arg Val Thr Val
465 470 475 480
Ser Thr Gln Met Ala Gly Arg Gly Thr Asp Ile Arg Leu Gly Gly Ala
485 490 495
Asp Glu Ala Asp Tyr Asp Glu Val Val Lys Leu Gly Gly Leu Ala Val
500 505 510
Ile Gly Thr Ala Arg His Arg Ser Gln Arg Leu Asp Asn Gln Leu Arg
515 520 525
Gly Arg Ala Gly Arg Gln Gly Asp Pro Gly Leu Ser Leu Phe Phe Val
530 535 540
Ser Leu Asp Asp Asp Val Val Val Ser Gly Gly Ser Arg Glu Ser Val
545 550 555 560
Ser Ala Gln Pro Asp Ala Thr Gly Leu Ile Asp Ser Asp Arg Ile Arg
565 570 575
Asp Trp Val Gly His Cys Gln Arg Val Thr Glu Gly Gln Leu Leu Glu
580 585 590
Ile His Ser Gln Ser Trp Asn Tyr Asn Lys Leu Leu Ala Asp Gln Arg
595 600 605
Val Ile Ile Asp Glu Arg Arg Glu Arg Leu Leu Asp Thr Ala Leu Ala
610 615 620
Trp Glu Glu Leu Ala Gln His Ala Pro Ala Arg Ala Ala Glu Leu Glu
625 630 635 640
Asp Leu Asp Gln Ser Val Arg Glu Gln Ala Ala Arg Asp Ile Met Leu
645 650 655
Tyr His Leu Asp Tyr Asn Trp Ser Glu His Leu Ala Leu Met Asp Asp
660 665 670
Val Arg Glu Ser Ile His Leu Arg Ala Ile Ala Arg Glu Thr Pro Leu
675 680 685
Asp Glu Tyr His Arg Ile Ala Val Arg Glu Phe Lys Asp Leu Ala Gln
690 695 700
Arg Ala Val Asp Asp Ala Val Ser Thr Phe Lys Ser Val Thr Ile Asp
705 710 715 720
His Glu Gly Ala His Leu Asp Asp Glu Gly Leu Ala Arg Pro Ser Ala
725 730 735
Thr Trp Thr Tyr Met Val Ser Asp Asn Pro Leu Ala Gly Ser Gly Asn
740 745 750
Ser Val Ile Ser Gly Ile Gly Asn Ile Phe Arg
755 760
<210> 69
<211> 1582
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1552)
<223> RXA02431
<400> 69
ggtggctgag cttgtcggca ttgtgctggt tgtcatcgca gttgctttgc gacgcccctc 60
ctagcggttt cccacaccgc cagtcttctc aaactaatcg ttg acc tgt tcg att 115
Leu Thr Cys Ser Ile
1 5
aac cta att ttc ggc tgg tca act acc ata aaa agc atg caa cgc tgg 163
Asn Leu Ile Phe Gly Trp Ser Thr Thr Ile Lys Ser Met Gln Arg Trp
10 15 20
gtg ctt cac atc gat atg gat gcc ttc ttc gca tcc tgc gaa caa ctg 211
Val Leu His Ile Asp Met Asp Ala Phe Phe Ala Ser Cys Glu Gln Leu
25 30 35
acc cgg ccc act tta aga ggc cgc ccc gtc ttg gtc ggt gga gtc tcc 259
Thr Arg Pro Thr Leu Arg Gly Arg Pro Val Leu Val Gly Gly Val Ser
40 45 50
ggt agg gga gtt gtc gcc gga gca tcc tat gaa gcc aga aaa ttt ggc 307
Gly Arg Gly Val Val Ala Gly Ala Ser Tyr Glu Ala Arg Lys Phe Gly
55 60 65
gcc cgc tca gcg atg ccc atg cac caa gcc aaa gcc cga gta ggt ttt 355
Ala Arg Ser Ala Met Pro Met His Gln Ala Lys Ala Arg Val Gly Phe
70 75 80 85
ggg gca gtg gtg gtg aca ccc cgt cat atc gtt tac tcc gca gcc tcg 403
Gly Ala Val Val Val Thr Pro Arg His Ile Val Tyr Ser Ala Ala Ser
90 95 100
cgc cgg gtg ttc caa atc gtg gaa aaa cgc gcc gga att gtc gaa cgc 451
Arg Arg Val Phe Gln Ile Val Glu Lys Arg Ala Gly Ile Val Glu Arg
105 110 115
ctc agc atc gat gaa ggc ttc atg gaa cca gag gct ctc gtt gga gcc 499
Leu Ser Ile Asp Glu Gly Phe Met Glu Pro Glu Ala Leu Val Gly Ala
120 125 130
acc cca gaa gag gtg aaa cag tgg gcg gaa gaa tta cgc gcg gaa att 547
Thr Pro Glu Glu Val Lys Gln Trp Ala Glu Glu Leu Arg Ala Glu Ile
135 140 145
aaa gaa gtt act ggc tta ccc tcc tcg gtt ggt gct ggc tcc ggt aag 595
Lys Glu Val Thr Gly Leu Pro Ser Ser Val Gly Ala Gly Ser Gly Lys
150 155 160 165
cag atc gcc aaa att ggt tca ggc gaa gca aag cca gat ggt gtg ttt 643
Gln Ile Ala Lys Ile Gly Ser Gly Glu Ala Lys Pro Asp Gly Val Phe
170 175 180
gtc gtg cca gta gac aag caa cat gac ttg ctt gat cca ctt cct gtg 691
Val Val Pro Val Asp Lys Gln His Asp Leu Leu Asp Pro Leu Pro Val
185 190 195
ggc gca ctt tgg gga gtg ggt cct gtg aca ggc tcc aag ctt gcc tca 739
Gly Ala Leu Trp Gly Val Gly Pro Val Thr Gly Ser Lys Leu Ala Ser
200 205 210
atg ggg gtg gaa aca att ggt gat cta gca gcg cta acc caa aaa gaa 787
Met Gly Val Glu Thr Ile Gly Asp Leu Ala Ala Leu Thr Gln Lys Glu
215 220 225
gta gaa atc agc ctc ggt gca acc atc gga ata tca ctg tgg aac ctt 835
Val Glu Ile Ser Leu Gly Ala Thr Ile Gly Ile Ser Leu Trp Asn Leu
230 235 240 245
gcc cga gga atc gac gac cgc cct gtg gaa ccc cgc gcc gaa gca aaa 883
Ala Arg Gly Ile Asp Asp Arg Pro Val Glu Pro Arg Ala Glu Ala Lys
250 255 260
cag atc tcc caa gag cac acc tat gaa aaa gac ctc ctc acc agg caa 931
Gln Ile Ser Gln Glu His Thr Tyr Glu Lys Asp Leu Leu Thr Arg Gln
265 270 275
caa gta gat gct gcc atc att cga tca gcc gaa ggc gca cac cga cgg 979
Gln Val Asp Ala Ala Ile Ile Arg Ser Ala Glu Gly Ala His Arg Arg
280 285 290
ctc ctc aaa gac gga cgc ggt gcc aga act gtc agc gtg aaa ctg cgg 1027
Leu Leu Lys Asp Gly Arg Gly Ala Arg Thr Val Ser Val Lys Leu Arg
295 300 305
atg gcc gac ttt cgt att gag tct cgt tcc tac acc ttg tcc tat gcc 1075
Met Ala Asp Phe Arg Ile Glu Ser Arg Ser Tyr Thr Leu Ser Tyr Ala
310 315 320 325
acc gat gat tac gca act ctt gag gca aca gca ttc cga ctt gcc cgc 1123
Thr Asp Asp Tyr Ala Thr Leu Glu Ala Thr Ala Phe Arg Leu Ala Arg
330 335 340
tac ccc gga gaa gta ggc ccc atc cgc ctt gtc gga gta agt ttt tct 1171
Tyr Pro Gly Glu Val Gly Pro Ile Arg Leu Val Gly Val Ser Phe Ser
345 350 355
ggt ttg gaa gaa tcc cgc caa gac atc ctc ttc ccg gaa ctt gac caa 1219
Gly Leu Glu Glu Ser Arg Gln Asp Ile Leu Phe Pro Glu Leu Asp Gln
360 365 370
caa atc atc gta cca cca gca ccc gac acc gat tat gag gta ggc gtg 1267
Gln Ile Ile Val Pro Pro Ala Pro Asp Thr Asp Tyr Glu Val Gly Val
375 380 385
caa tcc tct tct agt tcc gaa agt act caa gtt gaa gcg ccg caa gat 1315
Gln Ser Ser Ser Ser Ser Glu Ser Thr Gln Val Glu Ala Pro Gln Asp
390 395 400 405
gtc gcg ttg agt atg tgg tgc gca acg caa gat gtc tac cac cca gaa 1363
Val Ala Leu Ser Met Trp Cys Ala Thr Gln Asp Val Tyr His Pro Glu
410 415 420
tat ggc cac ggt tgg gta caa ggt gcc ggt cac ggt gtt gta tca gta 1411
Tyr Gly His Gly Trp Val Gln Gly Ala Gly His Gly Val Val Ser Val
425 430 435
cgt ttt gaa acc cgc agc acc aca aaa ggg cga act aaa agt ttt tcc 1459
Arg Phe Glu Thr Arg Ser Thr Thr Lys Gly Arg Thr Lys Ser Phe Ser
440 445 450
atg gat gac ccg gac ctc acc ccg gca gac cct cta gat agt ttg gat 1507
Met Asp Asp Pro Asp Leu Thr Pro Ala Asp Pro Leu Asp Ser Leu Asp
455 460 465
tgg gct gac tgg ttt gct gaa aat ggt gaa acg ggg gat gac gaa 1552
Trp Ala Asp Trp Phe Ala Glu Asn Gly Glu Thr Gly Asp Asp Glu
470 475 480
tagggtttca tcgggtttcg gggtgctttt 1582
<210> 70
<211> 484
<212> PRT
<213> Corynebacterium glutamicum
<400> 70
Leu Thr Cys Ser Ile Asn Leu Ile Phe Gly Trp Ser Thr Thr Ile Lys
1 5 10 15
Ser Met Gln Arg Trp Val Leu His Ile Asp Met Asp Ala Phe Phe Ala
20 25 30
Ser Cys Glu Gln Leu Thr Arg Pro Thr Leu Arg Gly Arg Pro Val Leu
35 40 45
Val Gly Gly Val Ser Gly Arg Gly Val Val Ala Gly Ala Ser Tyr Glu
50 55 60
Ala Arg Lys Phe Gly Ala Arg Ser Ala Met Pro Met His Gln Ala Lys
65 70 75 80
Ala Arg Val Gly Phe Gly Ala Val Val Val Thr Pro Arg His Ile Val
85 90 95
Tyr Ser Ala Ala Ser Arg Arg Val Phe Gln Ile Val Glu Lys Arg Ala
100 105 110
Gly Ile Val Glu Arg Leu Ser Ile Asp Glu Gly Phe Met Glu Pro Glu
115 120 125
Ala Leu Val Gly Ala Thr Pro Glu Glu Val Lys Gln Trp Ala Glu Glu
130 135 140
Leu Arg Ala Glu Ile Lys Glu Val Thr Gly Leu Pro Ser Ser Val Gly
145 150 155 160
Ala Gly Ser Gly Lys Gln Ile Ala Lys Ile Gly Ser Gly Glu Ala Lys
165 170 175
Pro Asp Gly Val Phe Val Val Pro Val Asp Lys Gln His Asp Leu Leu
180 185 190
Asp Pro Leu Pro Val Gly Ala Leu Trp Gly Val Gly Pro Val Thr Gly
195 200 205
Ser Lys Leu Ala Ser Met Gly Val Glu Thr Ile Gly Asp Leu Ala Ala
210 215 220
Leu Thr Gln Lys Glu Val Glu Ile Ser Leu Gly Ala Thr Ile Gly Ile
225 230 235 240
Ser Leu Trp Asn Leu Ala Arg Gly Ile Asp Asp Arg Pro Val Glu Pro
245 250 255
Arg Ala Glu Ala Lys Gln Ile Ser Gln Glu His Thr Tyr Glu Lys Asp
260 265 270
Leu Leu Thr Arg Gln Gln Val Asp Ala Ala Ile Ile Arg Ser Ala Glu
275 280 285
Gly Ala His Arg Arg Leu Leu Lys Asp Gly Arg Gly Ala Arg Thr Val
290 295 300
Ser Val Lys Leu Arg Met Ala Asp Phe Arg Ile Glu Ser Arg Ser Tyr
305 310 315 320
Thr Leu Ser Tyr Ala Thr Asp Asp Tyr Ala Thr Leu Glu Ala Thr Ala
325 330 335
Phe Arg Leu Ala Arg Tyr Pro Gly Glu Val Gly Pro Ile Arg Leu Val
340 345 350
Gly Val Ser Phe Ser Gly Leu Glu Glu Ser Arg Gln Asp Ile Leu Phe
355 360 365
Pro Glu Leu Asp Gln Gln Ile Ile Val Pro Pro Ala Pro Asp Thr Asp
370 375 380
Tyr Glu Val Gly Val Gln Ser Ser Ser Ser Ser Glu Ser Thr Gln Val
385 390 395 400
Glu Ala Pro Gln Asp Val Ala Leu Ser Met Trp Cys Ala Thr Gln Asp
405 410 415
Val Tyr His Pro Glu Tyr Gly His Gly Trp Val Gln Gly Ala Gly His
420 425 430
Gly Val Val Ser Val Arg Phe Glu Thr Arg Ser Thr Thr Lys Gly Arg
435 440 445
Thr Lys Ser Phe Ser Met Asp Asp Pro Asp Leu Thr Pro Ala Asp Pro
450 455 460
Leu Asp Ser Leu Asp Trp Ala Asp Trp Phe Ala Glu Asn Gly Glu Thr
465 470 475 480
Gly Asp Asp Glu
<210> 71
<211> 1819
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1789)
<223> RXA02445
<400> 71
tgtataaagt gacgctctca gtgtagaaac tgagggtctc agtgaagtag actgacaggg 60
atacctaata cgtttaatag attgtggggc actcgccacc gtg gaa cca ttc gaa 115
Val Glu Pro Phe Glu
1 5
tta gag aaa gac ctt gag cgt ctt agg aaa aac gga aaa gac gat gaa 163
Leu Glu Lys Asp Leu Glu Arg Leu Arg Lys Asn Gly Lys Asp Asp Glu
10 15 20
acc gta gaa gtg aaa tct tgg ggt cgg tta cct tta agc aaa gga tca 211
Thr Val Glu Val Lys Ser Trp Gly Arg Leu Pro Leu Ser Lys Gly Ser
25 30 35
aaa agc ttc tgg gaa tca tta agc gca ttc gca aac acc aac ggt gga 259
Lys Ser Phe Trp Glu Ser Leu Ser Ala Phe Ala Asn Thr Asn Gly Gly
40 45 50
tac atc cta ttg ggg cta agc gaa cca gat ttc act cca gtt gaa gga 307
Tyr Ile Leu Leu Gly Leu Ser Glu Pro Asp Phe Thr Pro Val Glu Gly
55 60 65
ttt gat tca cag gcg agt atc cag ttc att cgt gca ggt tta aat cca 355
Phe Asp Ser Gln Ala Ser Ile Gln Phe Ile Arg Ala Gly Leu Asn Pro
70 75 80 85
caa gat cgc gac gcc caa aaa gtg gaa cca gtg ccc cat cat gaa att 403
Gln Asp Arg Asp Ala Gln Lys Val Glu Pro Val Pro His His Glu Ile
90 95 100
cat gaa atg act gtt gat ggt gct gaa gtt gtt tta gtt tca gtc tca 451
His Glu Met Thr Val Asp Gly Ala Glu Val Val Leu Val Ser Val Ser
105 110 115
ccg ttg tca gtg aac ggg ccc tgt tat tat ctt ccc gtc gga atc act 499
Pro Leu Ser Val Asn Gly Pro Cys Tyr Tyr Leu Pro Val Gly Ile Thr
120 125 130
aat ggc agc ttc aaa cgc gtt ggc gat gaa gac cgg aag ctc agt cat 547
Asn Gly Ser Phe Lys Arg Val Gly Asp Glu Asp Arg Lys Leu Ser His
135 140 145
ctt gaa att tac gag ctc caa aat agg ttt gtt caa acc aaa aca gat 595
Leu Glu Ile Tyr Glu Leu Gln Asn Arg Phe Val Gln Thr Lys Thr Asp
150 155 160 165
aga aat cca gtt cca gat tca agc atc gac gat ctc aac aat cag ctc 643
Arg Asn Pro Val Pro Asp Ser Ser Ile Asp Asp Leu Asn Asn Gln Leu
170 175 180
gcg gcg tca ttt aag cag cgc cta att gag tca aat agt cgc tcc ctt 691
Ala Ala Ser Phe Lys Gln Arg Leu Ile Glu Ser Asn Ser Arg Ser Leu
185 190 195
gga aca gac gat aac tgg tta ctg cgc aaa aat atc act aca tca aag 739
Gly Thr Asp Asp Asn Trp Leu Leu Arg Lys Asn Ile Thr Thr Ser Lys
200 205 210
gga gaa ctg acg att gct ggc tta ctg gct ctc gga agc tat cct caa 787
Gly Glu Leu Thr Ile Ala Gly Leu Leu Ala Leu Gly Ser Tyr Pro Gln
215 220 225
cag ttt ttc ccc cga gtg atc att gat gtt gcc gta cat cca ggt ctg 835
Gln Phe Phe Pro Arg Val Ile Ile Asp Val Ala Val His Pro Gly Leu
230 235 240 245
cat aag tca cca atc ggt acc tca att cgt ttt gaa gac cga aaa atc 883
His Lys Ser Pro Ile Gly Thr Ser Ile Arg Phe Glu Asp Arg Lys Ile
250 255 260
tgc gag gga aat ctt ctc gag atg gtt caa gag gct atg tct gcc atc 931
Cys Glu Gly Asn Leu Leu Glu Met Val Gln Glu Ala Met Ser Ala Ile
265 270 275
aaa cga aac cta cgt gta cgc cgc gtc gtt gaa gga ctc tca ggt aaa 979
Lys Arg Asn Leu Arg Val Arg Arg Val Val Glu Gly Leu Ser Gly Lys
280 285 290
gat gtt cta gaa atc cca gaa gaa gtt ttg aga gag gct cta gca aac 1027
Asp Val Leu Glu Ile Pro Glu Glu Val Leu Arg Glu Ala Leu Ala Asn
295 300 305
gcc gta ctt cac cgt gat tat tct gag cta gct caa aat gaa gca att 1075
Ala Val Leu His Arg Asp Tyr Ser Glu Leu Ala Gln Asn Glu Ala Ile
310 315 320 325
cat gta gac atc tat aag gat cga gtt gag atc acg agt cca ggt gga 1123
His Val Asp Ile Tyr Lys Asp Arg Val Glu Ile Thr Ser Pro Gly Gly
330 335 340
tta ccc aat ggt aaa cgc cca gag tca ata ctg gac gga tac tct gaa 1171
Leu Pro Asn Gly Lys Arg Pro Glu Ser Ile Leu Asp Gly Tyr Ser Glu
345 350 355
cca aga aat cgt gtg ctt tca aga atc cta atg gat att cca tgg aca 1219
Pro Arg Asn Arg Val Leu Ser Arg Ile Leu Met Asp Ile Pro Trp Thr
360 365 370
cat gaa gta caa gga gta ctt gct gaa agc aac ggt act ggc gtt ccc 1267
His Glu Val Gln Gly Val Leu Ala Glu Ser Asn Gly Thr Gly Val Pro
375 380 385
cga atg ttc aat ttg atg cgt gaa gcg gga ctt ccg gta ccg aat ttt 1315
Arg Met Phe Asn Leu Met Arg Glu Ala Gly Leu Pro Val Pro Asn Phe
390 395 400 405
aag att gat att tct agc gtc act gtc gaa ctc agc cgt cac ggt ctt 1363
Lys Ile Asp Ile Ser Ser Val Thr Val Glu Leu Ser Arg His Gly Leu
410 415 420
cta gat gcc caa aca agt gaa tgg ctt gta gaa aaa ctc gga tca gat 1411
Leu Asp Ala Gln Thr Ser Glu Trp Leu Val Glu Lys Leu Gly Ser Asp
425 430 435
ttt tct aac aca caa ggc att gct ctt gtt ctc gca aaa gaa ctt gga 1459
Phe Ser Asn Thr Gln Gly Ile Ala Leu Val Leu Ala Lys Glu Leu Gly
440 445 450
gcg gta acg tct cga gat ctc cgc aat caa act ggt cat gat tca gaa 1507
Ala Val Thr Ser Arg Asp Leu Arg Asn Gln Thr Gly His Asp Ser Glu
455 460 465
gac atg cgc agc tta ctt gac gct ttg gtt gat cgg ggc gtt cta aac 1555
Asp Met Arg Ser Leu Leu Asp Ala Leu Val Asp Arg Gly Val Leu Asn
470 475 480 485
caa aac tta cag aac caa tat cag ctt gcg aca tcg tct gtg aat gta 1603
Gln Asn Leu Gln Asn Gln Tyr Gln Leu Ala Thr Ser Ser Val Asn Val
490 495 500
act caa agc gaa caa gaa gtc tta gat gca atc aat aaa aca act cct 1651
Thr Gln Ser Glu Gln Glu Val Leu Asp Ala Ile Asn Lys Thr Thr Pro
505 510 515
gtc aca att cga gaa att gcc aca aaa aca ggg aaa act gca tcg tct 1699
Val Thr Ile Arg Glu Ile Ala Thr Lys Thr Gly Lys Thr Ala Ser Ser
520 525 530
ctt cgg ccg ctg ctt cgt ggc ctt gtt gaa gca ggt ctt gtg gtt gca 1747
Leu Arg Pro Leu Leu Arg Gly Leu Val Glu Ala Gly Leu Val Val Ala
535 540 545
act gct cca cca tca agc cgc aac cga gcg tac ttg aag gct 1789
Thr Ala Pro Pro Ser Ser Arg Asn Arg Ala Tyr Leu Lys Ala
550 555 560
tgacccacca acgaactcac cggtgtcagc 1819
<210> 72
<211> 563
<212> PRT
<213> Corynebacterium glutamicum
<400> 72
Val Glu Pro Phe Glu Leu Glu Lys Asp Leu Glu Arg Leu Arg Lys Asn
1 5 10 15
Gly Lys Asp Asp Glu Thr Val Glu Val Lys Ser Trp Gly Arg Leu Pro
20 25 30
Leu Ser Lys Gly Ser Lys Ser Phe Trp Glu Ser Leu Ser Ala Phe Ala
35 40 45
Asn Thr Asn Gly Gly Tyr Ile Leu Leu Gly Leu Ser Glu Pro Asp Phe
50 55 60
Thr Pro Val Glu Gly Phe Asp Ser Gln Ala Ser Ile Gln Phe Ile Arg
65 70 75 80
Ala Gly Leu Asn Pro Gln Asp Arg Asp Ala Gln Lys Val Glu Pro Val
85 90 95
Pro His His Glu Ile His Glu Met Thr Val Asp Gly Ala Glu Val Val
100 105 110
Leu Val Ser Val Ser Pro Leu Ser Val Asn Gly Pro Cys Tyr Tyr Leu
115 120 125
Pro Val Gly Ile Thr Asn Gly Ser Phe Lys Arg Val Gly Asp Glu Asp
130 135 140
Arg Lys Leu Ser His Leu Glu Ile Tyr Glu Leu Gln Asn Arg Phe Val
145 150 155 160
Gln Thr Lys Thr Asp Arg Asn Pro Val Pro Asp Ser Ser Ile Asp Asp
165 170 175
Leu Asn Asn Gln Leu Ala Ala Ser Phe Lys Gln Arg Leu Ile Glu Ser
180 185 190
Asn Ser Arg Ser Leu Gly Thr Asp Asp Asn Trp Leu Leu Arg Lys Asn
195 200 205
Ile Thr Thr Ser Lys Gly Glu Leu Thr Ile Ala Gly Leu Leu Ala Leu
210 215 220
Gly Ser Tyr Pro Gln Gln Phe Phe Pro Arg Val Ile Ile Asp Val Ala
225 230 235 240
Val His Pro Gly Leu His Lys Ser Pro Ile Gly Thr Ser Ile Arg Phe
245 250 255
Glu Asp Arg Lys Ile Cys Glu Gly Asn Leu Leu Glu Met Val Gln Glu
260 265 270
Ala Met Ser Ala Ile Lys Arg Asn Leu Arg Val Arg Arg Val Val Glu
275 280 285
Gly Leu Ser Gly Lys Asp Val Leu Glu Ile Pro Glu Glu Val Leu Arg
290 295 300
Glu Ala Leu Ala Asn Ala Val Leu His Arg Asp Tyr Ser Glu Leu Ala
305 310 315 320
Gln Asn Glu Ala Ile His Val Asp Ile Tyr Lys Asp Arg Val Glu Ile
325 330 335
Thr Ser Pro Gly Gly Leu Pro Asn Gly Lys Arg Pro Glu Ser Ile Leu
340 345 350
Asp Gly Tyr Ser Glu Pro Arg Asn Arg Val Leu Ser Arg Ile Leu Met
355 360 365
Asp Ile Pro Trp Thr His Glu Val Gln Gly Val Leu Ala Glu Ser Asn
370 375 380
Gly Thr Gly Val Pro Arg Met Phe Asn Leu Met Arg Glu Ala Gly Leu
385 390 395 400
Pro Val Pro Asn Phe Lys Ile Asp Ile Ser Ser Val Thr Val Glu Leu
405 410 415
Ser Arg His Gly Leu Leu Asp Ala Gln Thr Ser Glu Trp Leu Val Glu
420 425 430
Lys Leu Gly Ser Asp Phe Ser Asn Thr Gln Gly Ile Ala Leu Val Leu
435 440 445
Ala Lys Glu Leu Gly Ala Val Thr Ser Arg Asp Leu Arg Asn Gln Thr
450 455 460
Gly His Asp Ser Glu Asp Met Arg Ser Leu Leu Asp Ala Leu Val Asp
465 470 475 480
Arg Gly Val Leu Asn Gln Asn Leu Gln Asn Gln Tyr Gln Leu Ala Thr
485 490 495
Ser Ser Val Asn Val Thr Gln Ser Glu Gln Glu Val Leu Asp Ala Ile
500 505 510
Asn Lys Thr Thr Pro Val Thr Ile Arg Glu Ile Ala Thr Lys Thr Gly
515 520 525
Lys Thr Ala Ser Ser Leu Arg Pro Leu Leu Arg Gly Leu Val Glu Ala
530 535 540
Gly Leu Val Val Ala Thr Ala Pro Pro Ser Ser Arg Asn Arg Ala Tyr
545 550 555 560
Leu Lys Ala
<210> 73
<211> 1009
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(979)
<223> RXA02476
<400> 73
cgggcggagt tctatcaaca ttacgcaaag gcataagctt tattattcca ctcggtgtga 60
catatgacct aaagtgccag tcagtacaat catttaggtc atg tca ttt aca gct 115
Met Ser Phe Thr Ala
1 5
ttt caa aca gcc ctg ctc gtg tgg ttt aga gca aat gcc cgc gat ctt 163
Phe Gln Thr Ala Leu Leu Val Trp Phe Arg Ala Asn Ala Arg Asp Leu
10 15 20
gcg tgg cgt gat ccc aat act tca gca tgg gga att ctc ctt tca gag 211
Ala Trp Arg Asp Pro Asn Thr Ser Ala Trp Gly Ile Leu Leu Ser Glu
25 30 35
gtg atg agc caa caa act ccc gtc gcg cga gtc gag ccg att tgg cgt 259
Val Met Ser Gln Gln Thr Pro Val Ala Arg Val Glu Pro Ile Trp Arg
40 45 50
gag tgg atg gaa aaa tgg ccc act ccg gaa gat ttc gcg aat gcg agc 307
Glu Trp Met Glu Lys Trp Pro Thr Pro Glu Asp Phe Ala Asn Ala Ser
55 60 65
acc gat gag att ttg cgg tcg tgg ggc aag ttg ggc tat cca cgt agg 355
Thr Asp Glu Ile Leu Arg Ser Trp Gly Lys Leu Gly Tyr Pro Arg Arg
70 75 80 85
gcg ctg agg ttg aag gaa tgt gcg gag gtg atc gtc gaa aag cat gcc 403
Ala Leu Arg Leu Lys Glu Cys Ala Glu Val Ile Val Glu Lys His Ala
90 95 100
ggc gag gtg ccg gat acg gtg gag gcg ctg ctc gcg ttg ccg ggg atc 451
Gly Glu Val Pro Asp Thr Val Glu Ala Leu Leu Ala Leu Pro Gly Ile
105 110 115
ggt gat tac acg gcg cgc gcg gtc gcg gcg ttt cat ttt ggg cag cgc 499
Gly Asp Tyr Thr Ala Arg Ala Val Ala Ala Phe His Phe Gly Gln Arg
120 125 130
gtg ccg gtg gtc gat acg aac gtg cgt cgc gtg tac cag cgc gcg gta 547
Val Pro Val Val Asp Thr Asn Val Arg Arg Val Tyr Gln Arg Ala Val
135 140 145
gcc gga cgt tac ctt gcg ggg cct gcg aaa aag caa gag ctt atc gac 595
Ala Gly Arg Tyr Leu Ala Gly Pro Ala Lys Lys Gln Glu Leu Ile Asp
150 155 160 165
gtc tcc ctt ctc ctt ccc aac act cac gcc cca gaa ttc tct gcc gca 643
Val Ser Leu Leu Leu Pro Asn Thr His Ala Pro Glu Phe Ser Ala Ala
170 175 180
ata atg gag ttg ggt gct ctt atc tgc acg gcc act tcc cca aag tgt 691
Ile Met Glu Leu Gly Ala Leu Ile Cys Thr Ala Thr Ser Pro Lys Cys
185 190 195
gac acc tgc cca ctg ctt gac cag tgt caa tgg caa aaa ctt ggc tgt 739
Asp Thr Cys Pro Leu Leu Asp Gln Cys Gln Trp Gln Lys Leu Gly Cys
200 205 210
ccc tcc ccg agt gaa gag gag ctg gct tca gcg aaa aag cgt gtg cag 787
Pro Ser Pro Ser Glu Glu Glu Leu Ala Ser Ala Lys Lys Arg Val Gln
215 220 225
aaa ttt gtg gga acc gac cga caa gtc cgt ggc cta atc atg gac gta 835
Lys Phe Val Gly Thr Asp Arg Gln Val Arg Gly Leu Ile Met Asp Val
230 235 240 245
ctg cgc aat gcc acc gca cct gtg cca cta tcc gcg att gat gtc gtg 883
Leu Arg Asn Ala Thr Ala Pro Val Pro Leu Ser Ala Ile Asp Val Val
250 255 260
tgg cct gac gat gcc caa cgc tcc cgg gcg ctg ttt tcg ctc att gag 931
Trp Pro Asp Asp Ala Gln Arg Ser Arg Ala Leu Phe Ser Leu Ile Glu
265 270 275
gac gga ctc gcg gaa caa aat gag gcg ggt tat ttc cac ctg cca cgg 979
Asp Gly Leu Ala Glu Gln Asn Glu Ala Gly Tyr Phe His Leu Pro Arg
280 285 290
taaaccactg cgcgcctgca aaaaacagta 1009
<210> 74
<211> 293
<212> PRT
<213> Corynebacterium glutamicum
<400> 74
Met Ser Phe Thr Ala Phe Gln Thr Ala Leu Leu Val Trp Phe Arg Ala
1 5 10 15
Asn Ala Arg Asp Leu Ala Trp Arg Asp Pro Asn Thr Ser Ala Trp Gly
20 25 30
Ile Leu Leu Ser Glu Val Met Ser Gln Gln Thr Pro Val Ala Arg Val
35 40 45
Glu Pro Ile Trp Arg Glu Trp Met Glu Lys Trp Pro Thr Pro Glu Asp
50 55 60
Phe Ala Asn Ala Ser Thr Asp Glu Ile Leu Arg Ser Trp Gly Lys Leu
65 70 75 80
Gly Tyr Pro Arg Arg Ala Leu Arg Leu Lys Glu Cys Ala Glu Val Ile
85 90 95
Val Glu Lys His Ala Gly Glu Val Pro Asp Thr Val Glu Ala Leu Leu
100 105 110
Ala Leu Pro Gly Ile Gly Asp Tyr Thr Ala Arg Ala Val Ala Ala Phe
115 120 125
His Phe Gly Gln Arg Val Pro Val Val Asp Thr Asn Val Arg Arg Val
130 135 140
Tyr Gln Arg Ala Val Ala Gly Arg Tyr Leu Ala Gly Pro Ala Lys Lys
145 150 155 160
Gln Glu Leu Ile Asp Val Ser Leu Leu Leu Pro Asn Thr His Ala Pro
165 170 175
Glu Phe Ser Ala Ala Ile Met Glu Leu Gly Ala Leu Ile Cys Thr Ala
180 185 190
Thr Ser Pro Lys Cys Asp Thr Cys Pro Leu Leu Asp Gln Cys Gln Trp
195 200 205
Gln Lys Leu Gly Cys Pro Ser Pro Ser Glu Glu Glu Leu Ala Ser Ala
210 215 220
Lys Lys Arg Val Gln Lys Phe Val Gly Thr Asp Arg Gln Val Arg Gly
225 230 235 240
Leu Ile Met Asp Val Leu Arg Asn Ala Thr Ala Pro Val Pro Leu Ser
245 250 255
Ala Ile Asp Val Val Trp Pro Asp Asp Ala Gln Arg Ser Arg Ala Leu
260 265 270
Phe Ser Leu Ile Glu Asp Gly Leu Ala Glu Gln Asn Glu Ala Gly Tyr
275 280 285
Phe His Leu Pro Arg
290
<210> 75
<211> 3319
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(3289)
<223> RXA02726
<400> 75
ttgtgtcaac gaagtggagc tagttaattt agctcaagct gggtggtacc gcgtccgttt 60
tttagggcgt ccccgcaggt agaacgataa ttattgttac ttg cgt gaa gga tgg 115
Leu Arg Glu Gly Trp
1 5
gac cga aca cac atg tct gaa gcc gtt ggc gga gtt tac cca cag gtt 163
Asp Arg Thr His Met Ser Glu Ala Val Gly Gly Val Tyr Pro Gln Val
10 15 20
gat tta tct ggt ggg tca tcc aga ttt cca gag atg gaa gag aat gta 211
Asp Leu Ser Gly Gly Ser Ser Arg Phe Pro Glu Met Glu Glu Asn Val
25 30 35
ctg agc tac tgg aag aag gat gac acc ttc cag gcc agc atc gat cag 259
Leu Ser Tyr Trp Lys Lys Asp Asp Thr Phe Gln Ala Ser Ile Asp Gln
40 45 50
cgc gat ggt gct gaa gac tac gtc ttt tac gat ggc cct cct ttt gca 307
Arg Asp Gly Ala Glu Asp Tyr Val Phe Tyr Asp Gly Pro Pro Phe Ala
55 60 65
aac ggt ctg cca cac tac ggc cac cta ctg act ggt tac gtc aag gac 355
Asn Gly Leu Pro His Tyr Gly His Leu Leu Thr Gly Tyr Val Lys Asp
70 75 80 85
att gtt cct cgc tac cag acc atg cgt ggc tac cgc gtt cct cgt gtc 403
Ile Val Pro Arg Tyr Gln Thr Met Arg Gly Tyr Arg Val Pro Arg Val
90 95 100
ttc ggt tgg gat acc cac ggt ctg cca gct gaa ctt gag gct gaa aag 451
Phe Gly Trp Asp Thr His Gly Leu Pro Ala Glu Leu Glu Ala Glu Lys
105 110 115
cag ctc ggc atc aag gac aag ggc gag atc gag gcc atg ggt ctt gcc 499
Gln Leu Gly Ile Lys Asp Lys Gly Glu Ile Glu Ala Met Gly Leu Ala
120 125 130
aag ttc aac gag tac tgt gca acc tcc gtg ttg cag tac acc aag gaa 547
Lys Phe Asn Glu Tyr Cys Ala Thr Ser Val Leu Gln Tyr Thr Lys Glu
135 140 145
tgg gaa gag tac gtc acc cgc cag gct cgt tgg gtg gac ttt gaa aac 595
Trp Glu Glu Tyr Val Thr Arg Gln Ala Arg Trp Val Asp Phe Glu Asn
150 155 160 165
ggc tac aag acc atg gac ctt tct ttc atg gag tcc gtg atc tgg gcg 643
Gly Tyr Lys Thr Met Asp Leu Ser Phe Met Glu Ser Val Ile Trp Ala
170 175 180
ttc aag gaa ctc tac gac aag ggc ctg atc tac cag ggt ttc cgc gtt 691
Phe Lys Glu Leu Tyr Asp Lys Gly Leu Ile Tyr Gln Gly Phe Arg Val
185 190 195
ctt cct tac tcc tgg gca gag cac acc cca ctg tcc aac cag gaa acc 739
Leu Pro Tyr Ser Trp Ala Glu His Thr Pro Leu Ser Asn Gln Glu Thr
200 205 210
cga ctg gat gac tcc tac aag ctg cgc cag gat cca acc ctg acc gtc 787
Arg Leu Asp Asp Ser Tyr Lys Leu Arg Gln Asp Pro Thr Leu Thr Val
215 220 225
acg ttc cca gtc acc ggt gtc gtc gaa ggt tct tct gca aac gct ggc 835
Thr Phe Pro Val Thr Gly Val Val Glu Gly Ser Ser Ala Asn Ala Gly
230 235 240 245
ctg gtg gga gcg ttg gct ctt gcg tgg acg act acc ccg tgg acc ctt 883
Leu Val Gly Ala Leu Ala Leu Ala Trp Thr Thr Thr Pro Trp Thr Leu
250 255 260
cca tcc aac ctt gcg ttg gct gtg aac cca gcg gtg acc tac gca ttg 931
Pro Ser Asn Leu Ala Leu Ala Val Asn Pro Ala Val Thr Tyr Ala Leu
265 270 275
gtt gag gtt gct gaa gac ggt gag gca gaa ttc gtc ggc aag cgt gtg 979
Val Glu Val Ala Glu Asp Gly Glu Ala Glu Phe Val Gly Lys Arg Val
280 285 290
ctt ttg gct aag gac ctc gtt ggt tcc tac gcc aag gaa ctc ggt gct 1027
Leu Leu Ala Lys Asp Leu Val Gly Ser Tyr Ala Lys Glu Leu Gly Ala
295 300 305
gag gct gtt atc gtt tct gag cac cca ggc tct gaa ctg gtc gga ctg 1075
Glu Ala Val Ile Val Ser Glu His Pro Gly Ser Glu Leu Val Gly Leu
310 315 320 325
acc tac gag cca atc ttt gga tat ttc cgc gat cac gcg aac gga ttc 1123
Thr Tyr Glu Pro Ile Phe Gly Tyr Phe Arg Asp His Ala Asn Gly Phe
330 335 340
cag atc ctc ggt gca gag tac gtc acc acc gaa gac ggc acc ggt atc 1171
Gln Ile Leu Gly Ala Glu Tyr Val Thr Thr Glu Asp Gly Thr Gly Ile
345 350 355
gtc cac cag gca cca gct ttc ggt gaa gac gat atg aac acc tgt aac 1219
Val His Gln Ala Pro Ala Phe Gly Glu Asp Asp Met Asn Thr Cys Asn
360 365 370
gct gcc ggc att gag cca gtc atc cca gtg gac atc gac ggc aag ttc 1267
Ala Ala Gly Ile Glu Pro Val Ile Pro Val Asp Ile Asp Gly Lys Phe
375 380 385
acc ggt ttg gtt cct gaa tac caa ggt cag ctt gtt ttc gat gcc aac 1315
Thr Gly Leu Val Pro Glu Tyr Gln Gly Gln Leu Val Phe Asp Ala Asn
390 395 400 405
aag gac atc atc aag gac ttg aag gct gca ggt cgc gtg gtt cgc cac 1363
Lys Asp Ile Ile Lys Asp Leu Lys Ala Ala Gly Arg Val Val Arg His
410 415 420
cag acc atc gaa cac tcc tac cca cac tct tgg cgt tcc ggt gag cca 1411
Gln Thr Ile Glu His Ser Tyr Pro His Ser Trp Arg Ser Gly Glu Pro
425 430 435
ctg atc tac atg gct ctg cca tct tgg ttt gtg aat gtc acc gaa atc 1459
Leu Ile Tyr Met Ala Leu Pro Ser Trp Phe Val Asn Val Thr Glu Ile
440 445 450
cgc gac cgc atg gtt gag gtc aac cag gac atc gag tgg atg cca gcg 1507
Arg Asp Arg Met Val Glu Val Asn Gln Asp Ile Glu Trp Met Pro Ala
455 460 465
cac atc cgc gac ggc cag ttc ggc aag tgg cta gaa ggt gcc cgc gac 1555
His Ile Arg Asp Gly Gln Phe Gly Lys Trp Leu Glu Gly Ala Arg Asp
470 475 480 485
tgg aac atc tcc cgt tcc cgt tac tgg ggt tca cca att cca gca tgg 1603
Trp Asn Ile Ser Arg Ser Arg Tyr Trp Gly Ser Pro Ile Pro Ala Trp
490 495 500
gtc tcc gac aac gac gaa tac cca cgc gtt gat gtt tat ggt tcc ctc 1651
Val Ser Asp Asn Asp Glu Tyr Pro Arg Val Asp Val Tyr Gly Ser Leu
505 510 515
gat gag ctt gag gct gac ttt ggc gtg cgt cca aag tcc ctg cac cgt 1699
Asp Glu Leu Glu Ala Asp Phe Gly Val Arg Pro Lys Ser Leu His Arg
520 525 530
cca gac atc gat gaa cta act cgt cca aac cca gac gat cca acc ggc 1747
Pro Asp Ile Asp Glu Leu Thr Arg Pro Asn Pro Asp Asp Pro Thr Gly
535 540 545
aag tcc acc atg cga cgc gtc acc gat gtt ttg gac gtg tgg ttc gac 1795
Lys Ser Thr Met Arg Arg Val Thr Asp Val Leu Asp Val Trp Phe Asp
550 555 560 565
tcc ggt tcc atg ccg ttt gcc cag gtg cac tac cca ttc gag aac aaa 1843
Ser Gly Ser Met Pro Phe Ala Gln Val His Tyr Pro Phe Glu Asn Lys
570 575 580
gaa tgg ttt gat acc cac gca cca gca gac ttc atc gtg gag tac atc 1891
Glu Trp Phe Asp Thr His Ala Pro Ala Asp Phe Ile Val Glu Tyr Ile
585 590 595
ggt cag acc cgc ggt tgg ttc tac ctg ctg cac gtg ctg tcc acc gca 1939
Gly Gln Thr Arg Gly Trp Phe Tyr Leu Leu His Val Leu Ser Thr Ala
600 605 610
ctg ttt gac cgc cca gct ttc aag aag gtt gtc gca cac ggc atc gtc 1987
Leu Phe Asp Arg Pro Ala Phe Lys Lys Val Val Ala His Gly Ile Val
615 620 625
ttg ggt gat gac gga ctg aag atg tcc aag tcc aag ggc aac tac ccg 2035
Leu Gly Asp Asp Gly Leu Lys Met Ser Lys Ser Lys Gly Asn Tyr Pro
630 635 640 645
aac gtc aac gag gtc ttc gac cgc gac ggt tcc gac gcc atg cgt tgg 2083
Asn Val Asn Glu Val Phe Asp Arg Asp Gly Ser Asp Ala Met Arg Trp
650 655 660
ttc ctc atg agt tcc cca atc ctg cgc ggc ggc aac ttg att gtc acc 2131
Phe Leu Met Ser Ser Pro Ile Leu Arg Gly Gly Asn Leu Ile Val Thr
665 670 675
gaa aag ggc atc cgc gaa ggt gtg cgc caa gca cag ctt cca atg tgg 2179
Glu Lys Gly Ile Arg Glu Gly Val Arg Gln Ala Gln Leu Pro Met Trp
680 685 690
aac gca tac tcc ttc ctg cag ctg tac acc tcc aag aac gca acc tgg 2227
Asn Ala Tyr Ser Phe Leu Gln Leu Tyr Thr Ser Lys Asn Ala Thr Trp
695 700 705
tca gtc gac tcc act gac gtg ctg gac cgc tac atc ctg gcg aag ctg 2275
Ser Val Asp Ser Thr Asp Val Leu Asp Arg Tyr Ile Leu Ala Lys Leu
710 715 720 725
cac gat ttg gtg gca gag acc cag gcg gca ctc gac ggc act gac att 2323
His Asp Leu Val Ala Glu Thr Gln Ala Ala Leu Asp Gly Thr Asp Ile
730 735 740
gca aag gct tgc gac ttg gtt cgt aac ttc tgt gat gcg ttg acc aac 2371
Ala Lys Ala Cys Asp Leu Val Arg Asn Phe Cys Asp Ala Leu Thr Asn
745 750 755
tgg tac gtg cgt cgt tcc cgc gac cgt ttc tgg gct ggt gat gaa gca 2419
Trp Tyr Val Arg Arg Ser Arg Asp Arg Phe Trp Ala Gly Asp Glu Ala
760 765 770
cac cca gag gct ttc aac acc ttg tac acc gtg ctg gaa acc ctc acc 2467
His Pro Glu Ala Phe Asn Thr Leu Tyr Thr Val Leu Glu Thr Leu Thr
775 780 785
cgc gtg gca gct cca ctg ctg cca atg acc acc gaa gtg atc tgg cgt 2515
Arg Val Ala Ala Pro Leu Leu Pro Met Thr Thr Glu Val Ile Trp Arg
790 795 800 805
gga ctg acc ggc gag cgt tct gtg cac ctg act gat ttc cca tcc gct 2563
Gly Leu Thr Gly Glu Arg Ser Val His Leu Thr Asp Phe Pro Ser Ala
810 815 820
gag tct ttc cca gca gat gct gat ttg gtt cgc acc atg gat gag atc 2611
Glu Ser Phe Pro Ala Asp Ala Asp Leu Val Arg Thr Met Asp Glu Ile
825 830 835
cgt ggc gtg tgc tct gcg gct tcc tct gtt cgt aag gct cac aag ctg 2659
Arg Gly Val Cys Ser Ala Ala Ser Ser Val Arg Lys Ala His Lys Leu
840 845 850
cgt aac cgt ctg cca ctt cca ggc ctg act gtt gct ctt cca gac tct 2707
Arg Asn Arg Leu Pro Leu Pro Gly Leu Thr Val Ala Leu Pro Asp Ser
855 860 865
gct cgc ctg gca gac ttc gct tcg atc atc cgc gat gag gtc aac gtg 2755
Ala Arg Leu Ala Asp Phe Ala Ser Ile Ile Arg Asp Glu Val Asn Val
870 875 880 885
aag aac gtg gat ctg acc tct gac gtg gat tcc gtg gga acc ttc gag 2803
Lys Asn Val Asp Leu Thr Ser Asp Val Asp Ser Val Gly Thr Phe Glu
890 895 900
gtt gtt gtt aac gct aag gtt gca ggt cct cgc ttg ggc aag gac gtc 2851
Val Val Val Asn Ala Lys Val Ala Gly Pro Arg Leu Gly Lys Asp Val
905 910 915
cag cgc gtg atc aag gct gtg aag gct ggc aac tac acc cgc gaa ggc 2899
Gln Arg Val Ile Lys Ala Val Lys Ala Gly Asn Tyr Thr Arg Glu Gly
920 925 930
gac gtc gtt gtt gcc gat ggc atc gag ctc aac gag ggt gaa ttc acc 2947
Asp Val Val Val Ala Asp Gly Ile Glu Leu Asn Glu Gly Glu Phe Thr
935 940 945
gag cgt ctc gta gca gca aac cct gat tcc acc gcg cag atc gac ggc 2995
Glu Arg Leu Val Ala Ala Asn Pro Asp Ser Thr Ala Gln Ile Asp Gly
950 955 960 965
gtg gat gga ctc gtg gtt ctg gac atg gaa gtc acg gaa gaa ctt gaa 3043
Val Asp Gly Leu Val Val Leu Asp Met Glu Val Thr Glu Glu Leu Glu
970 975 980
gca gaa ggc tgg gca gcg gac gcg atc cgt ggc ctg cag gat gct cga 3091
Ala Glu Gly Trp Ala Ala Asp Ala Ile Arg Gly Leu Gln Asp Ala Arg
985 990 995
aag aac tcc ggc ttt gag gtt tct gac cgc att tct gtt gtc gtc agc 3139
Lys Asn Ser Gly Phe Glu Val Ser Asp Arg Ile Ser Val Val Val Ser
1000 1005 1010
gtt cct gag gac aag aag gaa tgg atc acc act cac gct gat cac atc 3187
Val Pro Glu Asp Lys Lys Glu Trp Ile Thr Thr His Ala Asp His Ile
1015 1020 1025
gca gcg gaa gtt ttg gca acc tcc ttt gag atc gtc act gat gcc ctc 3235
Ala Ala Glu Val Leu Ala Thr Ser Phe Glu Ile Val Thr Asp Ala Leu
1030 1035 1040 1045
gac ggc gaa acc cac gac att gtc gct ggt gtg acc gcg aag gtt act 3283
Asp Gly Glu Thr His Asp Ile Val Ala Gly Val Thr Ala Lys Val Thr
1050 1055 1060
aag aac taagagttgt tttgttgaga aagcccgctg 3319
Lys Asn
<210> 76
<211> 1063
<212> PRT
<213> Corynebacterium glutamicum
<400> 76
Leu Arg Glu Gly Trp Asp Arg Thr His Met Ser Glu Ala Val Gly Gly
1 5 10 15
Val Tyr Pro Gln Val Asp Leu Ser Gly Gly Ser Ser Arg Phe Pro Glu
20 25 30
Met Glu Glu Asn Val Leu Ser Tyr Trp Lys Lys Asp Asp Thr Phe Gln
35 40 45
Ala Ser Ile Asp Gln Arg Asp Gly Ala Glu Asp Tyr Val Phe Tyr Asp
50 55 60
Gly Pro Pro Phe Ala Asn Gly Leu Pro His Tyr Gly His Leu Leu Thr
65 70 75 80
Gly Tyr Val Lys Asp Ile Val Pro Arg Tyr Gln Thr Met Arg Gly Tyr
85 90 95
Arg Val Pro Arg Val Phe Gly Trp Asp Thr His Gly Leu Pro Ala Glu
100 105 110
Leu Glu Ala Glu Lys Gln Leu Gly Ile Lys Asp Lys Gly Glu Ile Glu
115 120 125
Ala Met Gly Leu Ala Lys Phe Asn Glu Tyr Cys Ala Thr Ser Val Leu
130 135 140
Gln Tyr Thr Lys Glu Trp Glu Glu Tyr Val Thr Arg Gln Ala Arg Trp
145 150 155 160
Val Asp Phe Glu Asn Gly Tyr Lys Thr Met Asp Leu Ser Phe Met Glu
165 170 175
Ser Val Ile Trp Ala Phe Lys Glu Leu Tyr Asp Lys Gly Leu Ile Tyr
180 185 190
Gln Gly Phe Arg Val Leu Pro Tyr Ser Trp Ala Glu His Thr Pro Leu
195 200 205
Ser Asn Gln Glu Thr Arg Leu Asp Asp Ser Tyr Lys Leu Arg Gln Asp
210 215 220
Pro Thr Leu Thr Val Thr Phe Pro Val Thr Gly Val Val Glu Gly Ser
225 230 235 240
Ser Ala Asn Ala Gly Leu Val Gly Ala Leu Ala Leu Ala Trp Thr Thr
245 250 255
Thr Pro Trp Thr Leu Pro Ser Asn Leu Ala Leu Ala Val Asn Pro Ala
260 265 270
Val Thr Tyr Ala Leu Val Glu Val Ala Glu Asp Gly Glu Ala Glu Phe
275 280 285
Val Gly Lys Arg Val Leu Leu Ala Lys Asp Leu Val Gly Ser Tyr Ala
290 295 300
Lys Glu Leu Gly Ala Glu Ala Val Ile Val Ser Glu His Pro Gly Ser
305 310 315 320
Glu Leu Val Gly Leu Thr Tyr Glu Pro Ile Phe Gly Tyr Phe Arg Asp
325 330 335
His Ala Asn Gly Phe Gln Ile Leu Gly Ala Glu Tyr Val Thr Thr Glu
340 345 350
Asp Gly Thr Gly Ile Val His Gln Ala Pro Ala Phe Gly Glu Asp Asp
355 360 365
Met Asn Thr Cys Asn Ala Ala Gly Ile Glu Pro Val Ile Pro Val Asp
370 375 380
Ile Asp Gly Lys Phe Thr Gly Leu Val Pro Glu Tyr Gln Gly Gln Leu
385 390 395 400
Val Phe Asp Ala Asn Lys Asp Ile Ile Lys Asp Leu Lys Ala Ala Gly
405 410 415
Arg Val Val Arg His Gln Thr Ile Glu His Ser Tyr Pro His Ser Trp
420 425 430
Arg Ser Gly Glu Pro Leu Ile Tyr Met Ala Leu Pro Ser Trp Phe Val
435 440 445
Asn Val Thr Glu Ile Arg Asp Arg Met Val Glu Val Asn Gln Asp Ile
450 455 460
Glu Trp Met Pro Ala His Ile Arg Asp Gly Gln Phe Gly Lys Trp Leu
465 470 475 480
Glu Gly Ala Arg Asp Trp Asn Ile Ser Arg Ser Arg Tyr Trp Gly Ser
485 490 495
Pro Ile Pro Ala Trp Val Ser Asp Asn Asp Glu Tyr Pro Arg Val Asp
500 505 510
Val Tyr Gly Ser Leu Asp Glu Leu Glu Ala Asp Phe Gly Val Arg Pro
515 520 525
Lys Ser Leu His Arg Pro Asp Ile Asp Glu Leu Thr Arg Pro Asn Pro
530 535 540
Asp Asp Pro Thr Gly Lys Ser Thr Met Arg Arg Val Thr Asp Val Leu
545 550 555 560
Asp Val Trp Phe Asp Ser Gly Ser Met Pro Phe Ala Gln Val His Tyr
565 570 575
Pro Phe Glu Asn Lys Glu Trp Phe Asp Thr His Ala Pro Ala Asp Phe
580 585 590
Ile Val Glu Tyr Ile Gly Gln Thr Arg Gly Trp Phe Tyr Leu Leu His
595 600 605
Val Leu Ser Thr Ala Leu Phe Asp Arg Pro Ala Phe Lys Lys Val Val
610 615 620
Ala His Gly Ile Val Leu Gly Asp Asp Gly Leu Lys Met Ser Lys Ser
625 630 635 640
Lys Gly Asn Tyr Pro Asn Val Asn Glu Val Phe Asp Arg Asp Gly Ser
645 650 655
Asp Ala Met Arg Trp Phe Leu Met Ser Ser Pro Ile Leu Arg Gly Gly
660 665 670
Asn Leu Ile Val Thr Glu Lys Gly Ile Arg Glu Gly Val Arg Gln Ala
675 680 685
Gln Leu Pro Met Trp Asn Ala Tyr Ser Phe Leu Gln Leu Tyr Thr Ser
690 695 700
Lys Asn Ala Thr Trp Ser Val Asp Ser Thr Asp Val Leu Asp Arg Tyr
705 710 715 720
Ile Leu Ala Lys Leu His Asp Leu Val Ala Glu Thr Gln Ala Ala Leu
725 730 735
Asp Gly Thr Asp Ile Ala Lys Ala Cys Asp Leu Val Arg Asn Phe Cys
740 745 750
Asp Ala Leu Thr Asn Trp Tyr Val Arg Arg Ser Arg Asp Arg Phe Trp
755 760 765
Ala Gly Asp Glu Ala His Pro Glu Ala Phe Asn Thr Leu Tyr Thr Val
770 775 780
Leu Glu Thr Leu Thr Arg Val Ala Ala Pro Leu Leu Pro Met Thr Thr
785 790 795 800
Glu Val Ile Trp Arg Gly Leu Thr Gly Glu Arg Ser Val His Leu Thr
805 810 815
Asp Phe Pro Ser Ala Glu Ser Phe Pro Ala Asp Ala Asp Leu Val Arg
820 825 830
Thr Met Asp Glu Ile Arg Gly Val Cys Ser Ala Ala Ser Ser Val Arg
835 840 845
Lys Ala His Lys Leu Arg Asn Arg Leu Pro Leu Pro Gly Leu Thr Val
850 855 860
Ala Leu Pro Asp Ser Ala Arg Leu Ala Asp Phe Ala Ser Ile Ile Arg
865 870 875 880
Asp Glu Val Asn Val Lys Asn Val Asp Leu Thr Ser Asp Val Asp Ser
885 890 895
Val Gly Thr Phe Glu Val Val Val Asn Ala Lys Val Ala Gly Pro Arg
900 905 910
Leu Gly Lys Asp Val Gln Arg Val Ile Lys Ala Val Lys Ala Gly Asn
915 920 925
Tyr Thr Arg Glu Gly Asp Val Val Val Ala Asp Gly Ile Glu Leu Asn
930 935 940
Glu Gly Glu Phe Thr Glu Arg Leu Val Ala Ala Asn Pro Asp Ser Thr
945 950 955 960
Ala Gln Ile Asp Gly Val Asp Gly Leu Val Val Leu Asp Met Glu Val
965 970 975
Thr Glu Glu Leu Glu Ala Glu Gly Trp Ala Ala Asp Ala Ile Arg Gly
980 985 990
Leu Gln Asp Ala Arg Lys Asn Ser Gly Phe Glu Val Ser Asp Arg Ile
995 1000 1005
Ser Val Val Val Ser Val Pro Glu Asp Lys Lys Glu Trp Ile Thr Thr
1010 1015 1020
His Ala Asp His Ile Ala Ala Glu Val Leu Ala Thr Ser Phe Glu Ile
1025 1030 1035 1040
Val Thr Asp Ala Leu Asp Gly Glu Thr His Asp Ile Val Ala Gly Val
1045 1050 1055
Thr Ala Lys Val Thr Lys Asn
1060
<210> 77
<211> 2290
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2260)
<223> RXA02731
<400> 77
gttgcatcga gtccgcgggt ttcgcacgct tgatctaaat tcttgagggt tttccggccg 60
ttgtttgcgc taaacgtagg ggtcaagcgt cgaaaagcgc ttg ctt gca cgc tgt 115
Leu Leu Ala Arg Cys
1 5
ttt gct gcg ggc cgc aac gtg tcc acc ctg tgg cgt atc cta gaa tgc 163
Phe Ala Ala Gly Arg Asn Val Ser Thr Leu Trp Arg Ile Leu Glu Cys
10 15 20
atg gct ttt gct gct gaa cat cct gtc ctg tcc cac tct gag cac cgc 211
Met Ala Phe Ala Ala Glu His Pro Val Leu Ser His Ser Glu His Arg
25 30 35
ccg gtt ggt gaa atc gag cgt agc gat gac aaa ttt gtt gtc gtt agt 259
Pro Val Gly Glu Ile Glu Arg Ser Asp Asp Lys Phe Val Val Val Ser
40 45 50
gaa ttt gag cct gcg ggt gac cag cct gcg gct att aaa gag ctc gat 307
Glu Phe Glu Pro Ala Gly Asp Gln Pro Ala Ala Ile Lys Glu Leu Asp
55 60 65
gag cgc ttg gat cgc ggt gag cgg gac gtc gtt ttg atg ggt gct act 355
Glu Arg Leu Asp Arg Gly Glu Arg Asp Val Val Leu Met Gly Ala Thr
70 75 80 85
ggt acg ggt aag tcc gcg act gcg gcg tgg ttg atc gaa aag cag cag 403
Gly Thr Gly Lys Ser Ala Thr Ala Ala Trp Leu Ile Glu Lys Gln Gln
90 95 100
cgc ccc gct ttg gtg atg gcg ccg aat aag acg ctg gct gcg cag ttg 451
Arg Pro Ala Leu Val Met Ala Pro Asn Lys Thr Leu Ala Ala Gln Leu
105 110 115
gct aat gaa ttg cgg cag ctg ttg ccc aat aac gcg gtg gag tat ttc 499
Ala Asn Glu Leu Arg Gln Leu Leu Pro Asn Asn Ala Val Glu Tyr Phe
120 125 130
gtg tct tat tac gat tac tac cag cca gaa gcg tat atc gcg cag act 547
Val Ser Tyr Tyr Asp Tyr Tyr Gln Pro Glu Ala Tyr Ile Ala Gln Thr
135 140 145
gat acc tat att gaa aag gac tcc tcg att aat gag gat gtg gag cgt 595
Asp Thr Tyr Ile Glu Lys Asp Ser Ser Ile Asn Glu Asp Val Glu Arg
150 155 160 165
ctg cgt cac tcg gcg acg tcg tct ttg ctg agt agg cga gac gtc gtg 643
Leu Arg His Ser Ala Thr Ser Ser Leu Leu Ser Arg Arg Asp Val Val
170 175 180
gtt gtt agt tcg gtg tcg tgt att tat ggc ttg ggc act cca cag tct 691
Val Val Ser Ser Val Ser Cys Ile Tyr Gly Leu Gly Thr Pro Gln Ser
185 190 195
tat ctt gac cgt tcc gtt gtg ttg aac gtg ggg gag gag atc gac cgc 739
Tyr Leu Asp Arg Ser Val Val Leu Asn Val Gly Glu Glu Ile Asp Arg
200 205 210
gat cgc ttt ttg cgc cta ttg gta gat att caa tac gaa cgc aat gat 787
Asp Arg Phe Leu Arg Leu Leu Val Asp Ile Gln Tyr Glu Arg Asn Asp
215 220 225
gtg ggc ttt act cgt ggt gct ttc cgc gtg aag ggc gat acc gtg gac 835
Val Gly Phe Thr Arg Gly Ala Phe Arg Val Lys Gly Asp Thr Val Asp
230 235 240 245
atc atc ccg gcc tat gag gaa ttg gcg gtg cgc att gag ttt ttc ggt 883
Ile Ile Pro Ala Tyr Glu Glu Leu Ala Val Arg Ile Glu Phe Phe Gly
250 255 260
gat gaa att gat gcg ttg tac tac atc cat ccc ctg act ggt gac acc 931
Asp Glu Ile Asp Ala Leu Tyr Tyr Ile His Pro Leu Thr Gly Asp Thr
265 270 275
atc cgg cag gtg aat gag atc cgt att ttc cca gct acg cac tat gtt 979
Ile Arg Gln Val Asn Glu Ile Arg Ile Phe Pro Ala Thr His Tyr Val
280 285 290
gcg gga cct gag cgg atg gaa aag gca gtc gct gat att aag gcg gag 1027
Ala Gly Pro Glu Arg Met Glu Lys Ala Val Ala Asp Ile Lys Ala Glu
295 300 305
ttg gaa gtg cgc ctg gct gat ttg gag aac cgt ggc aag tta ttg gaa 1075
Leu Glu Val Arg Leu Ala Asp Leu Glu Asn Arg Gly Lys Leu Leu Glu
310 315 320 325
gcg cag cgt ctt agg atg cgt act gaa tat gac tta gaa atg atc gag 1123
Ala Gln Arg Leu Arg Met Arg Thr Glu Tyr Asp Leu Glu Met Ile Glu
330 335 340
cag gtt ggt ttc tgt tcg ggc att gag aac tat tct cgc cac att gat 1171
Gln Val Gly Phe Cys Ser Gly Ile Glu Asn Tyr Ser Arg His Ile Asp
345 350 355
gga cgt ggg gag gga acc gca ccg gcc acg ctg att gac tat ttc cca 1219
Gly Arg Gly Glu Gly Thr Ala Pro Ala Thr Leu Ile Asp Tyr Phe Pro
360 365 370
gag gat ttc ctc acc atc atc gat gag tct cac gtg aca gtc ccg cag 1267
Glu Asp Phe Leu Thr Ile Ile Asp Glu Ser His Val Thr Val Pro Gln
375 380 385
atc ggc ggc atg ttt gag ggc gat atg tcc cgt aaa cgt aac ctc gta 1315
Ile Gly Gly Met Phe Glu Gly Asp Met Ser Arg Lys Arg Asn Leu Val
390 395 400 405
gaa ttc ggt ttc cgc ctg cca tcc gcg atg gat aac cgc cca ttg acc 1363
Glu Phe Gly Phe Arg Leu Pro Ser Ala Met Asp Asn Arg Pro Leu Thr
410 415 420
tgg gag gag ttc gat gaa cgc cgt ggc caa acg gtg ttc atg tct gca 1411
Trp Glu Glu Phe Asp Glu Arg Arg Gly Gln Thr Val Phe Met Ser Ala
425 430 435
act cca ggc aag ttt gag atc gct gct gct gat ggt gag ttt gtg gag 1459
Thr Pro Gly Lys Phe Glu Ile Ala Ala Ala Asp Gly Glu Phe Val Glu
440 445 450
cag gtc att cgc cca aca ggt ctg gtg gat cca aag gtc acc gtc aag 1507
Gln Val Ile Arg Pro Thr Gly Leu Val Asp Pro Lys Val Thr Val Lys
455 460 465
cca acg aag ggg cag att gat gat ctg atc cat gaa att cgc caa cgc 1555
Pro Thr Lys Gly Gln Ile Asp Asp Leu Ile His Glu Ile Arg Gln Arg
470 475 480 485
acc gat aaa gat gag cgc gtt ttg gtc acc aca ttg acc aag aaa atg 1603
Thr Asp Lys Asp Glu Arg Val Leu Val Thr Thr Leu Thr Lys Lys Met
490 495 500
gct gag gat ctt act gat tac ctg ctg gaa aac ggc atc cgc gtg cgc 1651
Ala Glu Asp Leu Thr Asp Tyr Leu Leu Glu Asn Gly Ile Arg Val Arg
505 510 515
tac ctg cac tca gat att gat acc ttg cag cgt gtg gaa ttg ctg cgt 1699
Tyr Leu His Ser Asp Ile Asp Thr Leu Gln Arg Val Glu Leu Leu Arg
520 525 530
cag ctt cgc ctg ggc gaa tac gat gtg ttg gta ggt att aac ctg ctg 1747
Gln Leu Arg Leu Gly Glu Tyr Asp Val Leu Val Gly Ile Asn Leu Leu
535 540 545
cgt gag ggc ctt gac ctg cca gaa gtc tct ctg gtt gcg att ctc gac 1795
Arg Glu Gly Leu Asp Leu Pro Glu Val Ser Leu Val Ala Ile Leu Asp
550 555 560 565
gcc gac aag gaa ggc ttc ctg cgc tcc acc acc tca ctg att cag acc 1843
Ala Asp Lys Glu Gly Phe Leu Arg Ser Thr Thr Ser Leu Ile Gln Thr
570 575 580
att ggc cgc gcc gcc cga aat gtg tcc ggc gag gtc atc atg tac gcc 1891
Ile Gly Arg Ala Ala Arg Asn Val Ser Gly Glu Val Ile Met Tyr Ala
585 590 595
gac aag atc act gat tcg atg cag tat gcc atc gag gaa acc gat cga 1939
Asp Lys Ile Thr Asp Ser Met Gln Tyr Ala Ile Glu Glu Thr Asp Arg
600 605 610
cgc cgt gaa aag cag gtc gct tat aac aag gaa cac ggc atc gat ccg 1987
Arg Arg Glu Lys Gln Val Ala Tyr Asn Lys Glu His Gly Ile Asp Pro
615 620 625
cag ccg ctt cga aag aaa atc gcg gac atc ctc gac cag gtc tat gac 2035
Gln Pro Leu Arg Lys Lys Ile Ala Asp Ile Leu Asp Gln Val Tyr Asp
630 635 640 645
aat tcc gct gat gga gca gga cct tct gcc tct ggc gat gcg gca gtc 2083
Asn Ser Ala Asp Gly Ala Gly Pro Ser Ala Ser Gly Asp Ala Ala Val
650 655 660
gtg gct aaa cct gac gtg tct agc atg ccc gcc aaa gaa gtg caa aag 2131
Val Ala Lys Pro Asp Val Ser Ser Met Pro Ala Lys Glu Val Gln Lys
665 670 675
ctt atc gac gac ctc agc gct cag atg gct gcg gcc gcg cgg gag ctc 2179
Leu Ile Asp Asp Leu Ser Ala Gln Met Ala Ala Ala Ala Arg Glu Leu
680 685 690
aag ttc gag ctg gca ggg cgt ctg cga gat gag atc ttc gag ctc aag 2227
Lys Phe Glu Leu Ala Gly Arg Leu Arg Asp Glu Ile Phe Glu Leu Lys
695 700 705
aag gaa ctg aga ggt atc aag gat gcc ggc atc taagtcagct tgctcactta 2280
Lys Glu Leu Arg Gly Ile Lys Asp Ala Gly Ile
710 715 720
aagcttcgaa 2290
<210> 78
<211> 720
<212> PRT
<213> Corynebacterium glutamicum
<400> 78
Leu Leu Ala Arg Cys Phe Ala Ala Gly Arg Asn Val Ser Thr Leu Trp
1 5 10 15
Arg Ile Leu Glu Cys Met Ala Phe Ala Ala Glu His Pro Val Leu Ser
20 25 30
His Ser Glu His Arg Pro Val Gly Glu Ile Glu Arg Ser Asp Asp Lys
35 40 45
Phe Val Val Val Ser Glu Phe Glu Pro Ala Gly Asp Gln Pro Ala Ala
50 55 60
Ile Lys Glu Leu Asp Glu Arg Leu Asp Arg Gly Glu Arg Asp Val Val
65 70 75 80
Leu Met Gly Ala Thr Gly Thr Gly Lys Ser Ala Thr Ala Ala Trp Leu
85 90 95
Ile Glu Lys Gln Gln Arg Pro Ala Leu Val Met Ala Pro Asn Lys Thr
100 105 110
Leu Ala Ala Gln Leu Ala Asn Glu Leu Arg Gln Leu Leu Pro Asn Asn
115 120 125
Ala Val Glu Tyr Phe Val Ser Tyr Tyr Asp Tyr Tyr Gln Pro Glu Ala
130 135 140
Tyr Ile Ala Gln Thr Asp Thr Tyr Ile Glu Lys Asp Ser Ser Ile Asn
145 150 155 160
Glu Asp Val Glu Arg Leu Arg His Ser Ala Thr Ser Ser Leu Leu Ser
165 170 175
Arg Arg Asp Val Val Val Val Ser Ser Val Ser Cys Ile Tyr Gly Leu
180 185 190
Gly Thr Pro Gln Ser Tyr Leu Asp Arg Ser Val Val Leu Asn Val Gly
195 200 205
Glu Glu Ile Asp Arg Asp Arg Phe Leu Arg Leu Leu Val Asp Ile Gln
210 215 220
Tyr Glu Arg Asn Asp Val Gly Phe Thr Arg Gly Ala Phe Arg Val Lys
225 230 235 240
Gly Asp Thr Val Asp Ile Ile Pro Ala Tyr Glu Glu Leu Ala Val Arg
245 250 255
Ile Glu Phe Phe Gly Asp Glu Ile Asp Ala Leu Tyr Tyr Ile His Pro
260 265 270
Leu Thr Gly Asp Thr Ile Arg Gln Val Asn Glu Ile Arg Ile Phe Pro
275 280 285
Ala Thr His Tyr Val Ala Gly Pro Glu Arg Met Glu Lys Ala Val Ala
290 295 300
Asp Ile Lys Ala Glu Leu Glu Val Arg Leu Ala Asp Leu Glu Asn Arg
305 310 315 320
Gly Lys Leu Leu Glu Ala Gln Arg Leu Arg Met Arg Thr Glu Tyr Asp
325 330 335
Leu Glu Met Ile Glu Gln Val Gly Phe Cys Ser Gly Ile Glu Asn Tyr
340 345 350
Ser Arg His Ile Asp Gly Arg Gly Glu Gly Thr Ala Pro Ala Thr Leu
355 360 365
Ile Asp Tyr Phe Pro Glu Asp Phe Leu Thr Ile Ile Asp Glu Ser His
370 375 380
Val Thr Val Pro Gln Ile Gly Gly Met Phe Glu Gly Asp Met Ser Arg
385 390 395 400
Lys Arg Asn Leu Val Glu Phe Gly Phe Arg Leu Pro Ser Ala Met Asp
405 410 415
Asn Arg Pro Leu Thr Trp Glu Glu Phe Asp Glu Arg Arg Gly Gln Thr
420 425 430
Val Phe Met Ser Ala Thr Pro Gly Lys Phe Glu Ile Ala Ala Ala Asp
435 440 445
Gly Glu Phe Val Glu Gln Val Ile Arg Pro Thr Gly Leu Val Asp Pro
450 455 460
Lys Val Thr Val Lys Pro Thr Lys Gly Gln Ile Asp Asp Leu Ile His
465 470 475 480
Glu Ile Arg Gln Arg Thr Asp Lys Asp Glu Arg Val Leu Val Thr Thr
485 490 495
Leu Thr Lys Lys Met Ala Glu Asp Leu Thr Asp Tyr Leu Leu Glu Asn
500 505 510
Gly Ile Arg Val Arg Tyr Leu His Ser Asp Ile Asp Thr Leu Gln Arg
515 520 525
Val Glu Leu Leu Arg Gln Leu Arg Leu Gly Glu Tyr Asp Val Leu Val
530 535 540
Gly Ile Asn Leu Leu Arg Glu Gly Leu Asp Leu Pro Glu Val Ser Leu
545 550 555 560
Val Ala Ile Leu Asp Ala Asp Lys Glu Gly Phe Leu Arg Ser Thr Thr
565 570 575
Ser Leu Ile Gln Thr Ile Gly Arg Ala Ala Arg Asn Val Ser Gly Glu
580 585 590
Val Ile Met Tyr Ala Asp Lys Ile Thr Asp Ser Met Gln Tyr Ala Ile
595 600 605
Glu Glu Thr Asp Arg Arg Arg Glu Lys Gln Val Ala Tyr Asn Lys Glu
610 615 620
His Gly Ile Asp Pro Gln Pro Leu Arg Lys Lys Ile Ala Asp Ile Leu
625 630 635 640
Asp Gln Val Tyr Asp Asn Ser Ala Asp Gly Ala Gly Pro Ser Ala Ser
645 650 655
Gly Asp Ala Ala Val Val Ala Lys Pro Asp Val Ser Ser Met Pro Ala
660 665 670
Lys Glu Val Gln Lys Leu Ile Asp Asp Leu Ser Ala Gln Met Ala Ala
675 680 685
Ala Ala Arg Glu Leu Lys Phe Glu Leu Ala Gly Arg Leu Arg Asp Glu
690 695 700
Ile Phe Glu Leu Lys Lys Glu Leu Arg Gly Ile Lys Asp Ala Gly Ile
705 710 715 720
<210> 79
<211> 1087
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1057)
<223> RXA02736
<400> 79
cagaggatta cccagcgggt acgtggggtc caaagagcgc tgatgaaatg ctttcccgca 60
acggtcacac ctggcgcagg ccataattta ggggcaaaaa atg atc ttt gaa ctt 115
Met Ile Phe Glu Leu
1 5
ccg gat acc acc acc cag caa att tcc aag acc cta act cga ctg cgt 163
Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr Leu Thr Arg Leu Arg
10 15 20
gaa tcg ggc acc cag gtc acc acc ggc cga gtg ctc acc ctc atc gtg 211
Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val Leu Thr Leu Ile Val
25 30 35
gtc act gac tcc gaa agc gat gtc gct gca gtt acc gag tcc acc aat 259
Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val Thr Glu Ser Thr Asn
40 45 50
gaa gcc tcg cgc gag cac cca tct cgc gtg atc att ttg gtg gtt ggc 307
Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile Ile Leu Val Val Gly
55 60 65
gat aaa act gca gaa aac aaa gtt gac gca gaa gtc cgt atc ggt ggc 355
Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu Val Arg Ile Gly Gly
70 75 80 85
gac gct ggt gct tcc gag atg atc atc atg cat ctc aac gga cct gtc 403
Asp Ala Gly Ala Ser Glu Met Ile Ile Met His Leu Asn Gly Pro Val
90 95 100
gct gac aag ctc cag tat gtc gtc aca cca ctg ttg ctt cct gac acc 451
Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu Leu Leu Pro Asp Thr
105 110 115
ccc atc gtt gct tgg tgg cca ggt gaa tca cca aag aat cct tcc cag 499
Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro Lys Asn Pro Ser Gln
120 125 130
gac cca att gga cgc atc gca caa cga cgc atc act gat gct ttg tac 547
Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile Thr Asp Ala Leu Tyr
135 140 145
gac cgt gat gac gca cta gaa gat cgt gtt gag aac tat cac cca ggt 595
Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu Asn Tyr His Pro Gly
150 155 160 165
gat acc gac atg acg tgg gcg cgc ctt acc cag tgg cgg gga ctt gtt 643
Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln Trp Arg Gly Leu Val
170 175 180
gcc tcc tca ttg gat cac cca cca cac agc gaa atc act tcc gtg agg 691
Ala Ser Ser Leu Asp His Pro Pro His Ser Glu Ile Thr Ser Val Arg
185 190 195
ctg acc ggt gca agc ggc agt acc tcg gtg gat ttg gct gca ggc tgg 739
Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp Leu Ala Ala Gly Trp
200 205 210
ttg gcg cgg agg ctg aaa gtg cct gtg atc cgc gag gtg aca gat gct 787
Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg Glu Val Thr Asp Ala
215 220 225
ccc acc gtg cca acc gat gag ttt ggt act cca ctg ctg gct atc cag 835
Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro Leu Leu Ala Ile Gln
230 235 240 245
cgc ctg gag atc gtt cgc acc acc ggc tcg atc atc atc acc atc tat 883
Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile Ile Ile Thr Ile Tyr
250 255 260
gac gct cat acc ctt cag gta gag atg ccg gaa tcc ggc aat gcc cca 931
Asp Ala His Thr Leu Gln Val Glu Met Pro Glu Ser Gly Asn Ala Pro
265 270 275
tcg ctg gtg gct att ggt cgt cga agt gag tcc gac tgc ttg tct gag 979
Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser Asp Cys Leu Ser Glu
280 285 290
gag ctt cgc cac atg gat cca gat ttg ggc tac cag cac gca cta tcc 1027
Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr Gln His Ala Leu Ser
295 300 305
ggc ttg tcc agc gtc aag ctg gaa acc gtc taaggagaaa tacaacacta 1077
Gly Leu Ser Ser Val Lys Leu Glu Thr Val
310 315
tggttgatgt 1087
<210> 80
<211> 319
<212> PRT
<213> Corynebacterium glutamicum
<400> 80
Met Ile Phe Glu Leu Pro Asp Thr Thr Thr Gln Gln Ile Ser Lys Thr
1 5 10 15
Leu Thr Arg Leu Arg Glu Ser Gly Thr Gln Val Thr Thr Gly Arg Val
20 25 30
Leu Thr Leu Ile Val Val Thr Asp Ser Glu Ser Asp Val Ala Ala Val
35 40 45
Thr Glu Ser Thr Asn Glu Ala Ser Arg Glu His Pro Ser Arg Val Ile
50 55 60
Ile Leu Val Val Gly Asp Lys Thr Ala Glu Asn Lys Val Asp Ala Glu
65 70 75 80
Val Arg Ile Gly Gly Asp Ala Gly Ala Ser Glu Met Ile Ile Met His
85 90 95
Leu Asn Gly Pro Val Ala Asp Lys Leu Gln Tyr Val Val Thr Pro Leu
100 105 110
Leu Leu Pro Asp Thr Pro Ile Val Ala Trp Trp Pro Gly Glu Ser Pro
115 120 125
Lys Asn Pro Ser Gln Asp Pro Ile Gly Arg Ile Ala Gln Arg Arg Ile
130 135 140
Thr Asp Ala Leu Tyr Asp Arg Asp Asp Ala Leu Glu Asp Arg Val Glu
145 150 155 160
Asn Tyr His Pro Gly Asp Thr Asp Met Thr Trp Ala Arg Leu Thr Gln
165 170 175
Trp Arg Gly Leu Val Ala Ser Ser Leu Asp His Pro Pro His Ser Glu
180 185 190
Ile Thr Ser Val Arg Leu Thr Gly Ala Ser Gly Ser Thr Ser Val Asp
195 200 205
Leu Ala Ala Gly Trp Leu Ala Arg Arg Leu Lys Val Pro Val Ile Arg
210 215 220
Glu Val Thr Asp Ala Pro Thr Val Pro Thr Asp Glu Phe Gly Thr Pro
225 230 235 240
Leu Leu Ala Ile Gln Arg Leu Glu Ile Val Arg Thr Thr Gly Ser Ile
245 250 255
Ile Ile Thr Ile Tyr Asp Ala His Thr Leu Gln Val Glu Met Pro Glu
260 265 270
Ser Gly Asn Ala Pro Ser Leu Val Ala Ile Gly Arg Arg Ser Glu Ser
275 280 285
Asp Cys Leu Ser Glu Glu Leu Arg His Met Asp Pro Asp Leu Gly Tyr
290 295 300
Gln His Ala Leu Ser Gly Leu Ser Ser Val Lys Leu Glu Thr Val
305 310 315
<210> 81
<211> 2479
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2449)
<223> RXA02742
<400> 81
gtatcggtaa aaggcccaat caaggattct tggttgggtc tttttccatg cttttcatgc 60
ttcgaacagt ccagaatagc cctgacctag acttagagct atg tcc gaa caa aga 115
Met Ser Glu Gln Arg
1 5
ctc gat cag ctt gag cga cgg ctt tct gaa ctg gaa cgg gag atc gcc 163
Leu Asp Gln Leu Glu Arg Arg Leu Ser Glu Leu Glu Arg Glu Ile Ala
10 15 20
gcg att cgt cag gag atc cgc cag gaa cgc cta gtg ctt ccg gaa ccg 211
Ala Ile Arg Gln Glu Ile Arg Gln Glu Arg Leu Val Leu Pro Glu Pro
25 30 35
gaa cct gtg aaa gtt gat aca gtc atc gcc acc gaa gcg acc gga gtc 259
Glu Pro Val Lys Val Asp Thr Val Ile Ala Thr Glu Ala Thr Gly Val
40 45 50
aat gca tcg tcg ggt ccg gag gcg aag atc gct ttg ttc atg gag agg 307
Asn Ala Ser Ser Gly Pro Glu Ala Lys Ile Ala Leu Phe Met Glu Arg
55 60 65
ttt agt ggt cgc cac gat gtg tat gcg cgg cgc tgg acc agc aga aaa 355
Phe Ser Gly Arg His Asp Val Tyr Ala Arg Arg Trp Thr Ser Arg Lys
70 75 80 85
acg ggc aaa agt gga tgg tcg ccg gct act cgc cag ggt ttt tac tca 403
Thr Gly Lys Ser Gly Trp Ser Pro Ala Thr Arg Gln Gly Phe Tyr Ser
90 95 100
aaa gac acc aca ccg aag gac tat ctc ccc ttc acc gtt gac acc gtc 451
Lys Asp Thr Thr Pro Lys Asp Tyr Leu Pro Phe Thr Val Asp Thr Val
105 110 115
aat gcg cat ctg cgc cgg ggc ggc gac cat atc ggt ctc tat gtg atg 499
Asn Ala His Leu Arg Arg Gly Gly Asp His Ile Gly Leu Tyr Val Met
120 125 130
gtc ccc atc gac acg tgc aaa ctt ctc gcc tgc gat ttc gac gat ggc 547
Val Pro Ile Asp Thr Cys Lys Leu Leu Ala Cys Asp Phe Asp Asp Gly
135 140 145
acc tgg aag caa gat gcg gcc gct ttc gtg tca gcc tgc acc gac cac 595
Thr Trp Lys Gln Asp Ala Ala Ala Phe Val Ser Ala Cys Thr Asp His
150 155 160 165
gga atc gat gcg ttg gct gaa att tct cga tcc gac gac ggc gcc ccc 643
Gly Ile Asp Ala Leu Ala Glu Ile Ser Arg Ser Asp Asp Gly Ala Pro
170 175 180
gtg tgg ata ttt ttc gat acc cca atc tcc gcg atg ctg gct cgg cgc 691
Val Trp Ile Phe Phe Asp Thr Pro Ile Ser Ala Met Leu Ala Arg Arg
185 190 195
cta ggt ttt gcc atg ctc cgc caa gcc atg aac tcc cgc cct gac atg 739
Leu Gly Phe Ala Met Leu Arg Gln Ala Met Asn Ser Arg Pro Asp Met
200 205 210
gat atg tct tct tat gat cgc ttc ttc cct gct caa gac acc atc gca 787
Asp Met Ser Ser Tyr Asp Arg Phe Phe Pro Ala Gln Asp Thr Ile Ala
215 220 225
acg cgc gca aac gga agc gca cgg ctg gga aat ttg atc gcg ctg ccc 835
Thr Arg Ala Asn Gly Ser Ala Arg Leu Gly Asn Leu Ile Ala Leu Pro
230 235 240 245
ctc aac ggc gac tgt cga gcc cgc aac acc gcc gtc ttc gcc gat tcg 883
Leu Asn Gly Asp Cys Arg Ala Arg Asn Thr Ala Val Phe Ala Asp Ser
250 255 260
gaa acg tgg gtt ccc ttc gaa gat cct ttc gca gcg ctc gcg gcc atc 931
Glu Thr Trp Val Pro Phe Glu Asp Pro Phe Ala Ala Leu Ala Ala Ile
265 270 275
acg cca cta gcc acc gaa aaa atc gag cag atc ctt gcc acc acg cag 979
Thr Pro Leu Ala Thr Glu Lys Ile Glu Gln Ile Leu Ala Thr Thr Gln
280 285 290
gaa aaa ttt ggc ccc gaa ccc gaa cac atc aaa cgc ccc acc cgc gcc 1027
Glu Lys Phe Gly Pro Glu Pro Glu His Ile Lys Arg Pro Thr Arg Ala
295 300 305
gaa ctc aaa cag gtt aaa gcc aac ggc gaa acc atc aaa ctc acc atc 1075
Glu Leu Lys Gln Val Lys Ala Asn Gly Glu Thr Ile Lys Leu Thr Ile
310 315 320 325
acc aac gag ctg agc gtc ccc acc gaa agg tta ccc gcg gcc gtc atc 1123
Thr Asn Glu Leu Ser Val Pro Thr Glu Arg Leu Pro Ala Ala Val Ile
330 335 340
gcg gag att aaa cac cgg gcg gta atc cca aac cct gag ttt tat cgt 1171
Ala Glu Ile Lys His Arg Ala Val Ile Pro Asn Pro Glu Phe Tyr Arg
345 350 355
cga caa gcg caa aga ttt tcg acc ttc ggc gtg ccg cgc atc gtc atc 1219
Arg Gln Ala Gln Arg Phe Ser Thr Phe Gly Val Pro Arg Ile Val Ile
360 365 370
cgc ttc gcc cag gcc gag cag cgc ttg ctg ctc cca cgc ggg ctt gtc 1267
Arg Phe Ala Gln Ala Glu Gln Arg Leu Leu Leu Pro Arg Gly Leu Val
375 380 385
gac gac acc ctc cgg atc ctc acc ctc gcc ggg tac aaa gtc agc gtc 1315
Asp Asp Thr Leu Arg Ile Leu Thr Leu Ala Gly Tyr Lys Val Ser Val
390 395 400 405
atc tgg cct cgg caa act cgg aaa acc atc gac gcg tct ttc gag ggc 1363
Ile Trp Pro Arg Gln Thr Arg Lys Thr Ile Asp Ala Ser Phe Glu Gly
410 415 420
gaa ttg cga tcc atg caa caa gag gga atc gac tcg ctc aaa ggc caa 1411
Glu Leu Arg Ser Met Gln Gln Glu Gly Ile Asp Ser Leu Lys Gly Gln
425 430 435
cgc acc ggc gta ttg gta gca ccg ccg ggc gct gga aaa aca gtg atg 1459
Arg Thr Gly Val Leu Val Ala Pro Pro Gly Ala Gly Lys Thr Val Met
440 445 450
gcc tgt gca ctc atc gcg aac aga aaa atc ccc acc gca gtg ata gtc 1507
Ala Cys Ala Leu Ile Ala Asn Arg Lys Ile Pro Thr Ala Val Ile Val
455 460 465
aac cgt gca gaa ttg att tcc caa tgg cgg gat cgt ctc gcg caa tac 1555
Asn Arg Ala Glu Leu Ile Ser Gln Trp Arg Asp Arg Leu Ala Gln Tyr
470 475 480 485
ctg agc atc gac gca gac tcc atc gga cag atc ggc gcg ggc cga cgc 1603
Leu Ser Ile Asp Ala Asp Ser Ile Gly Gln Ile Gly Ala Gly Arg Arg
490 495 500
aaa acc acc gga att atc gat ctc atc acc gtc caa tcc ttg agc cgt 1651
Lys Thr Thr Gly Ile Ile Asp Leu Ile Thr Val Gln Ser Leu Ser Arg
505 510 515
aaa gat tcc gat ccg aaa att ttg gaa caa tac ggc caa atc atc gtc 1699
Lys Asp Ser Asp Pro Lys Ile Leu Glu Gln Tyr Gly Gln Ile Ile Val
520 525 530
gac gag tgc cac aac atc gca gcc cca ggc gcc gaa gcc gca ttg aac 1747
Asp Glu Cys His Asn Ile Ala Ala Pro Gly Ala Glu Ala Ala Leu Asn
535 540 545
cag gtc aag gcc ccc tac tgg ctg ggt cta acc gcc acg ccg ttt cgt 1795
Gln Val Lys Ala Pro Tyr Trp Leu Gly Leu Thr Ala Thr Pro Phe Arg
550 555 560 565
tca gac cac atg gat gaa atc atc acc atg cag tgc ggt cct gtg cgc 1843
Ser Asp His Met Asp Glu Ile Ile Thr Met Gln Cys Gly Pro Val Arg
570 575 580
cac cgc atg gaa gtg gca aca gac aat gaa cag cgc ttg att cac atc 1891
His Arg Met Glu Val Ala Thr Asp Asn Glu Gln Arg Leu Ile His Ile
585 590 595
cac gaa acc tct ttc gac tct gag gaa acc acc gaa atc cag gat ctc 1939
His Glu Thr Ser Phe Asp Ser Glu Glu Thr Thr Glu Ile Gln Asp Leu
600 605 610
tac aat gag ctc gcg gtc gat tct gcc cga aat gcg caa atc act gcc 1987
Tyr Asn Glu Leu Ala Val Asp Ser Ala Arg Asn Ala Gln Ile Thr Ala
615 620 625
gaa gtg cac aaa gcg ctt gaa gct ggc gac cga tgt cta gtt ttg gtc 2035
Glu Val His Lys Ala Leu Glu Ala Gly Asp Arg Cys Leu Val Leu Val
630 635 640 645
aac cga att gca gcc ctt gaa gca ctg acc agc agt att acc gaa tct 2083
Asn Arg Ile Ala Ala Leu Glu Ala Leu Thr Ser Ser Ile Thr Glu Ser
650 655 660
ggc gat cac act gtc tta gtg atg cat ggc cgc caa acc caa gag gag 2131
Gly Asp His Thr Val Leu Val Met His Gly Arg Gln Thr Gln Glu Glu
665 670 675
cga gtt cac ctt cgt gcg caa ctt gcc tca ttg agt gaa aag cag gat 2179
Arg Val His Leu Arg Ala Gln Leu Ala Ser Leu Ser Glu Lys Gln Asp
680 685 690
ccg ttt gta ctg gtc gcg atg aat aaa gtc gcc ggc gaa ggc ctt gac 2227
Pro Phe Val Leu Val Ala Met Asn Lys Val Ala Gly Glu Gly Leu Asp
695 700 705
atc ccc agc ctc aac acg ctg ttt ttg gca gcg ccg gtg tcc ttc aag 2275
Ile Pro Ser Leu Asn Thr Leu Phe Leu Ala Ala Pro Val Ser Phe Lys
710 715 720 725
ggg ctg gtg att cag caa atc ggc cga gtt act cgc gca acc ggt gat 2323
Gly Leu Val Ile Gln Gln Ile Gly Arg Val Thr Arg Ala Thr Gly Asp
730 735 740
caa aac gct cct ccg gtg act gcc acg gtc cat gat ttt gtt gat tcc 2371
Gln Asn Ala Pro Pro Val Thr Ala Thr Val His Asp Phe Val Asp Ser
745 750 755
aag att ccg aca ctc aaa cgc atg cac ggt cgc cga ttg cgg gct atg 2419
Lys Ile Pro Thr Leu Lys Arg Met His Gly Arg Arg Leu Arg Ala Met
760 765 770
caa aag gaa gga ttc gct gtt tcg gag cct tgaggaggac cagaccaaac 2469
Gln Lys Glu Gly Phe Ala Val Ser Glu Pro
775 780
cagcgtgccc 2479
<210> 82
<211> 783
<212> PRT
<213> Corynebacterium glutamicum
<400> 82
Met Ser Glu Gln Arg Leu Asp Gln Leu Glu Arg Arg Leu Ser Glu Leu
1 5 10 15
Glu Arg Glu Ile Ala Ala Ile Arg Gln Glu Ile Arg Gln Glu Arg Leu
20 25 30
Val Leu Pro Glu Pro Glu Pro Val Lys Val Asp Thr Val Ile Ala Thr
35 40 45
Glu Ala Thr Gly Val Asn Ala Ser Ser Gly Pro Glu Ala Lys Ile Ala
50 55 60
Leu Phe Met Glu Arg Phe Ser Gly Arg His Asp Val Tyr Ala Arg Arg
65 70 75 80
Trp Thr Ser Arg Lys Thr Gly Lys Ser Gly Trp Ser Pro Ala Thr Arg
85 90 95
Gln Gly Phe Tyr Ser Lys Asp Thr Thr Pro Lys Asp Tyr Leu Pro Phe
100 105 110
Thr Val Asp Thr Val Asn Ala His Leu Arg Arg Gly Gly Asp His Ile
115 120 125
Gly Leu Tyr Val Met Val Pro Ile Asp Thr Cys Lys Leu Leu Ala Cys
130 135 140
Asp Phe Asp Asp Gly Thr Trp Lys Gln Asp Ala Ala Ala Phe Val Ser
145 150 155 160
Ala Cys Thr Asp His Gly Ile Asp Ala Leu Ala Glu Ile Ser Arg Ser
165 170 175
Asp Asp Gly Ala Pro Val Trp Ile Phe Phe Asp Thr Pro Ile Ser Ala
180 185 190
Met Leu Ala Arg Arg Leu Gly Phe Ala Met Leu Arg Gln Ala Met Asn
195 200 205
Ser Arg Pro Asp Met Asp Met Ser Ser Tyr Asp Arg Phe Phe Pro Ala
210 215 220
Gln Asp Thr Ile Ala Thr Arg Ala Asn Gly Ser Ala Arg Leu Gly Asn
225 230 235 240
Leu Ile Ala Leu Pro Leu Asn Gly Asp Cys Arg Ala Arg Asn Thr Ala
245 250 255
Val Phe Ala Asp Ser Glu Thr Trp Val Pro Phe Glu Asp Pro Phe Ala
260 265 270
Ala Leu Ala Ala Ile Thr Pro Leu Ala Thr Glu Lys Ile Glu Gln Ile
275 280 285
Leu Ala Thr Thr Gln Glu Lys Phe Gly Pro Glu Pro Glu His Ile Lys
290 295 300
Arg Pro Thr Arg Ala Glu Leu Lys Gln Val Lys Ala Asn Gly Glu Thr
305 310 315 320
Ile Lys Leu Thr Ile Thr Asn Glu Leu Ser Val Pro Thr Glu Arg Leu
325 330 335
Pro Ala Ala Val Ile Ala Glu Ile Lys His Arg Ala Val Ile Pro Asn
340 345 350
Pro Glu Phe Tyr Arg Arg Gln Ala Gln Arg Phe Ser Thr Phe Gly Val
355 360 365
Pro Arg Ile Val Ile Arg Phe Ala Gln Ala Glu Gln Arg Leu Leu Leu
370 375 380
Pro Arg Gly Leu Val Asp Asp Thr Leu Arg Ile Leu Thr Leu Ala Gly
385 390 395 400
Tyr Lys Val Ser Val Ile Trp Pro Arg Gln Thr Arg Lys Thr Ile Asp
405 410 415
Ala Ser Phe Glu Gly Glu Leu Arg Ser Met Gln Gln Glu Gly Ile Asp
420 425 430
Ser Leu Lys Gly Gln Arg Thr Gly Val Leu Val Ala Pro Pro Gly Ala
435 440 445
Gly Lys Thr Val Met Ala Cys Ala Leu Ile Ala Asn Arg Lys Ile Pro
450 455 460
Thr Ala Val Ile Val Asn Arg Ala Glu Leu Ile Ser Gln Trp Arg Asp
465 470 475 480
Arg Leu Ala Gln Tyr Leu Ser Ile Asp Ala Asp Ser Ile Gly Gln Ile
485 490 495
Gly Ala Gly Arg Arg Lys Thr Thr Gly Ile Ile Asp Leu Ile Thr Val
500 505 510
Gln Ser Leu Ser Arg Lys Asp Ser Asp Pro Lys Ile Leu Glu Gln Tyr
515 520 525
Gly Gln Ile Ile Val Asp Glu Cys His Asn Ile Ala Ala Pro Gly Ala
530 535 540
Glu Ala Ala Leu Asn Gln Val Lys Ala Pro Tyr Trp Leu Gly Leu Thr
545 550 555 560
Ala Thr Pro Phe Arg Ser Asp His Met Asp Glu Ile Ile Thr Met Gln
565 570 575
Cys Gly Pro Val Arg His Arg Met Glu Val Ala Thr Asp Asn Glu Gln
580 585 590
Arg Leu Ile His Ile His Glu Thr Ser Phe Asp Ser Glu Glu Thr Thr
595 600 605
Glu Ile Gln Asp Leu Tyr Asn Glu Leu Ala Val Asp Ser Ala Arg Asn
610 615 620
Ala Gln Ile Thr Ala Glu Val His Lys Ala Leu Glu Ala Gly Asp Arg
625 630 635 640
Cys Leu Val Leu Val Asn Arg Ile Ala Ala Leu Glu Ala Leu Thr Ser
645 650 655
Ser Ile Thr Glu Ser Gly Asp His Thr Val Leu Val Met His Gly Arg
660 665 670
Gln Thr Gln Glu Glu Arg Val His Leu Arg Ala Gln Leu Ala Ser Leu
675 680 685
Ser Glu Lys Gln Asp Pro Phe Val Leu Val Ala Met Asn Lys Val Ala
690 695 700
Gly Glu Gly Leu Asp Ile Pro Ser Leu Asn Thr Leu Phe Leu Ala Ala
705 710 715 720
Pro Val Ser Phe Lys Gly Leu Val Ile Gln Gln Ile Gly Arg Val Thr
725 730 735
Arg Ala Thr Gly Asp Gln Asn Ala Pro Pro Val Thr Ala Thr Val His
740 745 750
Asp Phe Val Asp Ser Lys Ile Pro Thr Leu Lys Arg Met His Gly Arg
755 760 765
Arg Leu Arg Ala Met Gln Lys Glu Gly Phe Ala Val Ser Glu Pro
770 775 780
<210> 83
<211> 1771
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1741)
<223> RXA02748
<400> 83
caggtcgttg gcaggtagct gacgtgacct gagcgggggc aaccggctaa catgtaggga 60
ttagcaaccc cgcactctag aacctactgg gagttcattc gtg ttt gag tca ctg 115
Val Phe Glu Ser Leu
1 5
tcc gat cgg ttg aat agc gcg ctt tcc ggc ctg cgc ggc aaa gga aag 163
Ser Asp Arg Leu Asn Ser Ala Leu Ser Gly Leu Arg Gly Lys Gly Lys
10 15 20
ctc acc gag gca gac atc aat gca acc aca cgc gag atc cgt ctc gcg 211
Leu Thr Glu Ala Asp Ile Asn Ala Thr Thr Arg Glu Ile Arg Leu Ala
25 30 35
ctg ctg gaa gct gac gtt tca tta acg gtt gtt cgt gcc ttc att aac 259
Leu Leu Glu Ala Asp Val Ser Leu Thr Val Val Arg Ala Phe Ile Asn
40 45 50
cga atc aag gaa cgc gcc gct ggt gca gaa gtt tct cag gca ctc aac 307
Arg Ile Lys Glu Arg Ala Ala Gly Ala Glu Val Ser Gln Ala Leu Asn
55 60 65
ccc gcg cag caa gtc atc aag atc gtc aac gag gaa ctg gtt cag atc 355
Pro Ala Gln Gln Val Ile Lys Ile Val Asn Glu Glu Leu Val Gln Ile
70 75 80 85
ctc ggt ggc gaa acc cgc cga ctg tca ctg gcc aaa aac cca ccg acc 403
Leu Gly Gly Glu Thr Arg Arg Leu Ser Leu Ala Lys Asn Pro Pro Thr
90 95 100
gtc atc atg ctg gca ggt ctg cag ggt gca ggt aag acc acc ctc gca 451
Val Ile Met Leu Ala Gly Leu Gln Gly Ala Gly Lys Thr Thr Leu Ala
105 110 115
ggt aaa ctg tcc aag cac ctg gtc aag cag ggt cac act cct atg ctt 499
Gly Lys Leu Ser Lys His Leu Val Lys Gln Gly His Thr Pro Met Leu
120 125 130
gtt gcc tgt gac ctt cag cgt cca ggc gca gtt cag cag ctg caa att 547
Val Ala Cys Asp Leu Gln Arg Pro Gly Ala Val Gln Gln Leu Gln Ile
135 140 145
gtg ggt gaa cgc gca ggc gtt acc act ttc gca ccg gat cca ggc acc 595
Val Gly Glu Arg Ala Gly Val Thr Thr Phe Ala Pro Asp Pro Gly Thr
150 155 160 165
agc atc gac tcc ctc gag cac gaa atg ggc acc tcc cac ggt gat cca 643
Ser Ile Asp Ser Leu Glu His Glu Met Gly Thr Ser His Gly Asp Pro
170 175 180
gtc gag gta gcg cgc gca ggt atc gaa gaa gcc aag cgc acc cag cac 691
Val Glu Val Ala Arg Ala Gly Ile Glu Glu Ala Lys Arg Thr Gln His
185 190 195
gac atc gtg atc gtg gat acc gca ggt cgc ctc ggt atc gat gaa acc 739
Asp Ile Val Ile Val Asp Thr Ala Gly Arg Leu Gly Ile Asp Glu Thr
200 205 210
ctg atg act cag gca cgc aac atc cgc gaa gcc atc aac cct gat gaa 787
Leu Met Thr Gln Ala Arg Asn Ile Arg Glu Ala Ile Asn Pro Asp Glu
215 220 225
gtg ctc ttt gtc att gac tcc atg att ggt caa gac gcc gta gac acc 835
Val Leu Phe Val Ile Asp Ser Met Ile Gly Gln Asp Ala Val Asp Thr
230 235 240 245
gcc gaa gca ttc cgc gac ggc gtc gac ttc acc ggt gtt gtc ctg acc 883
Ala Glu Ala Phe Arg Asp Gly Val Asp Phe Thr Gly Val Val Leu Thr
250 255 260
aag ctt gat ggc gac gcc cgc ggt ggt gct gca cta tcc atc cgt gaa 931
Lys Leu Asp Gly Asp Ala Arg Gly Gly Ala Ala Leu Ser Ile Arg Glu
265 270 275
gtc acc ggc aag ccc atc atg ttt gcc tcc act ggt gaa aaa ctc gac 979
Val Thr Gly Lys Pro Ile Met Phe Ala Ser Thr Gly Glu Lys Leu Asp
280 285 290
gac ttc gac gtc ttc cac cca gag cgc atg gcc agc cga atc ctg ggc 1027
Asp Phe Asp Val Phe His Pro Glu Arg Met Ala Ser Arg Ile Leu Gly
295 300 305
atg ggt gac gta ctg tca ctc atc gag cag gcc gaa gca gtc atg gat 1075
Met Gly Asp Val Leu Ser Leu Ile Glu Gln Ala Glu Ala Val Met Asp
310 315 320 325
cag gaa aag gca gag gtc gct gcc cag aag ttg ggc tcc ggc gag ctc 1123
Gln Glu Lys Ala Glu Val Ala Ala Gln Lys Leu Gly Ser Gly Glu Leu
330 335 340
acc ctg gaa gac ttc ctt gac caa atg ctg atg atc cgc cgc atg gga 1171
Thr Leu Glu Asp Phe Leu Asp Gln Met Leu Met Ile Arg Arg Met Gly
345 350 355
cca atc ggc aac atc ctc aag atg ctg cct ggt ggc aag cag atg tcc 1219
Pro Ile Gly Asn Ile Leu Lys Met Leu Pro Gly Gly Lys Gln Met Ser
360 365 370
caa atg gcg gac atg gtt gat gag aag caa ctc gac cgc atc cag gcg 1267
Gln Met Ala Asp Met Val Asp Glu Lys Gln Leu Asp Arg Ile Gln Ala
375 380 385
att atc cgc ggt atg acc ccg gcc gag cgc gat aat cca aag atc ctc 1315
Ile Ile Arg Gly Met Thr Pro Ala Glu Arg Asp Asn Pro Lys Ile Leu
390 395 400 405
aac gct tcc agg cgc aag cgc atc gcc aac ggt tcc ggt gtg acc gtg 1363
Asn Ala Ser Arg Arg Lys Arg Ile Ala Asn Gly Ser Gly Val Thr Val
410 415 420
tcc gaa gta aac aaa ctt gtt gaa cgc ttc ttc gag gct cgc aag atg 1411
Ser Glu Val Asn Lys Leu Val Glu Arg Phe Phe Glu Ala Arg Lys Met
425 430 435
atg ggt caa atg gct ggc cag ttt ggc atg ggt cct gga tcc cgc agt 1459
Met Gly Gln Met Ala Gly Gln Phe Gly Met Gly Pro Gly Ser Arg Ser
440 445 450
gca acc aag aag caa gcc aag ggc cgc aag ggt aag aac ggc aag cgt 1507
Ala Thr Lys Lys Gln Ala Lys Gly Arg Lys Gly Lys Asn Gly Lys Arg
455 460 465
aaa cca gcc aag aag ggc cca acc cag cca aag atg cca atg ggc ggt 1555
Lys Pro Ala Lys Lys Gly Pro Thr Gln Pro Lys Met Pro Met Gly Gly
470 475 480 485
atg cca gga atg cct ggg atg ccg ggt atg ggt gga gcc gga atg cct 1603
Met Pro Gly Met Pro Gly Met Pro Gly Met Gly Gly Ala Gly Met Pro
490 495 500
gac ctt gct gaa cta cag aag cag ctt ggt gga gca ggt ggc ggt atg 1651
Asp Leu Ala Glu Leu Gln Lys Gln Leu Gly Gly Ala Gly Gly Gly Met
505 510 515
gga ggc ctt ggt ggc gga ctc ccg ggc atg cca aag ccg cct aaa ggc 1699
Gly Gly Leu Gly Gly Gly Leu Pro Gly Met Pro Lys Pro Pro Lys Gly
520 525 530
atg gag aac ata gat ctc aac aac cta gac ttc ggt aag aag 1741
Met Glu Asn Ile Asp Leu Asn Asn Leu Asp Phe Gly Lys Lys
535 540 545
taactttgct ttagttggtc ggcgcatcac 1771
<210> 84
<211> 547
<212> PRT
<213> Corynebacterium glutamicum
<400> 84
Val Phe Glu Ser Leu Ser Asp Arg Leu Asn Ser Ala Leu Ser Gly Leu
1 5 10 15
Arg Gly Lys Gly Lys Leu Thr Glu Ala Asp Ile Asn Ala Thr Thr Arg
20 25 30
Glu Ile Arg Leu Ala Leu Leu Glu Ala Asp Val Ser Leu Thr Val Val
35 40 45
Arg Ala Phe Ile Asn Arg Ile Lys Glu Arg Ala Ala Gly Ala Glu Val
50 55 60
Ser Gln Ala Leu Asn Pro Ala Gln Gln Val Ile Lys Ile Val Asn Glu
65 70 75 80
Glu Leu Val Gln Ile Leu Gly Gly Glu Thr Arg Arg Leu Ser Leu Ala
85 90 95
Lys Asn Pro Pro Thr Val Ile Met Leu Ala Gly Leu Gln Gly Ala Gly
100 105 110
Lys Thr Thr Leu Ala Gly Lys Leu Ser Lys His Leu Val Lys Gln Gly
115 120 125
His Thr Pro Met Leu Val Ala Cys Asp Leu Gln Arg Pro Gly Ala Val
130 135 140
Gln Gln Leu Gln Ile Val Gly Glu Arg Ala Gly Val Thr Thr Phe Ala
145 150 155 160
Pro Asp Pro Gly Thr Ser Ile Asp Ser Leu Glu His Glu Met Gly Thr
165 170 175
Ser His Gly Asp Pro Val Glu Val Ala Arg Ala Gly Ile Glu Glu Ala
180 185 190
Lys Arg Thr Gln His Asp Ile Val Ile Val Asp Thr Ala Gly Arg Leu
195 200 205
Gly Ile Asp Glu Thr Leu Met Thr Gln Ala Arg Asn Ile Arg Glu Ala
210 215 220
Ile Asn Pro Asp Glu Val Leu Phe Val Ile Asp Ser Met Ile Gly Gln
225 230 235 240
Asp Ala Val Asp Thr Ala Glu Ala Phe Arg Asp Gly Val Asp Phe Thr
245 250 255
Gly Val Val Leu Thr Lys Leu Asp Gly Asp Ala Arg Gly Gly Ala Ala
260 265 270
Leu Ser Ile Arg Glu Val Thr Gly Lys Pro Ile Met Phe Ala Ser Thr
275 280 285
Gly Glu Lys Leu Asp Asp Phe Asp Val Phe His Pro Glu Arg Met Ala
290 295 300
Ser Arg Ile Leu Gly Met Gly Asp Val Leu Ser Leu Ile Glu Gln Ala
305 310 315 320
Glu Ala Val Met Asp Gln Glu Lys Ala Glu Val Ala Ala Gln Lys Leu
325 330 335
Gly Ser Gly Glu Leu Thr Leu Glu Asp Phe Leu Asp Gln Met Leu Met
340 345 350
Ile Arg Arg Met Gly Pro Ile Gly Asn Ile Leu Lys Met Leu Pro Gly
355 360 365
Gly Lys Gln Met Ser Gln Met Ala Asp Met Val Asp Glu Lys Gln Leu
370 375 380
Asp Arg Ile Gln Ala Ile Ile Arg Gly Met Thr Pro Ala Glu Arg Asp
385 390 395 400
Asn Pro Lys Ile Leu Asn Ala Ser Arg Arg Lys Arg Ile Ala Asn Gly
405 410 415
Ser Gly Val Thr Val Ser Glu Val Asn Lys Leu Val Glu Arg Phe Phe
420 425 430
Glu Ala Arg Lys Met Met Gly Gln Met Ala Gly Gln Phe Gly Met Gly
435 440 445
Pro Gly Ser Arg Ser Ala Thr Lys Lys Gln Ala Lys Gly Arg Lys Gly
450 455 460
Lys Asn Gly Lys Arg Lys Pro Ala Lys Lys Gly Pro Thr Gln Pro Lys
465 470 475 480
Met Pro Met Gly Gly Met Pro Gly Met Pro Gly Met Pro Gly Met Gly
485 490 495
Gly Ala Gly Met Pro Asp Leu Ala Glu Leu Gln Lys Gln Leu Gly Gly
500 505 510
Ala Gly Gly Gly Met Gly Gly Leu Gly Gly Gly Leu Pro Gly Met Pro
515 520 525
Lys Pro Pro Lys Gly Met Glu Asn Ile Asp Leu Asn Asn Leu Asp Phe
530 535 540
Gly Lys Lys
545
<210> 85
<211> 958
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(928)
<223> RXA03070
<400> 85
gtggataaaa gggaaaacat aggggtcatg aaatagaaca agcacgaggc ctggtaaata 60
cgaattcgac caagaaaacg taaacacccc aggagtactc gtg cct gcc ctt cca 115
Val Pro Ala Leu Pro
1 5
tca tct atc atc gac ccc ctc tgg cgc cag ttc tcc gcc tta atc cca 163
Ser Ser Ile Ile Asp Pro Leu Trp Arg Gln Phe Ser Ala Leu Ile Pro
10 15 20
ccg gtt atc atc acc cac cca cta ggg tgc cac cgt gca cgc att gct 211
Pro Val Ile Ile Thr His Pro Leu Gly Cys His Arg Ala Arg Ile Ala
25 30 35
gac cgg atc atc gtc gac aaa ctc atc gca gtg ctt gtc ctc ggt gtc 259
Asp Arg Ile Ile Val Asp Lys Leu Ile Ala Val Leu Val Leu Gly Val
40 45 50
tcc tat atc aag att tcc gat tcc acc tgc tca gcc acc acg ata cgc 307
Ser Tyr Ile Lys Ile Ser Asp Ser Thr Cys Ser Ala Thr Thr Ile Arg
55 60 65
acc cgc cga gac gag tgg atc act gcc ggg att ttc aag aat tta gaa 355
Thr Arg Arg Asp Glu Trp Ile Thr Ala Gly Ile Phe Lys Asn Leu Glu
70 75 80 85
cag atc tgt ctg gag tcc tac gac cgt ttc atc ggg tta gac cta gaa 403
Gln Ile Cys Leu Glu Ser Tyr Asp Arg Phe Ile Gly Leu Asp Leu Glu
90 95 100
aac tta aat gtt gat ggc tgc att gtt aaa gct ccc tgc ggc gga gag 451
Asn Leu Asn Val Asp Gly Cys Ile Val Lys Ala Pro Cys Gly Gly Glu
105 110 115
gta gcc ggc aga ttc ccg gtt gac cgg gaa aaa ggc acc aaa cgc tcg 499
Val Ala Gly Arg Phe Pro Val Asp Arg Glu Lys Gly Thr Lys Arg Ser
120 125 130
tta atg gtc gat gga cat gga atc ccg atc ggg tgc gtg gtc gcc gga 547
Leu Met Val Asp Gly His Gly Ile Pro Ile Gly Cys Val Val Ala Gly
135 140 145
gcc aat cgg cat gat tta ccg ttg tta gct gca acc ttg gac acg ctc 595
Ala Asn Arg His Asp Leu Pro Leu Leu Ala Ala Thr Leu Asp Thr Leu
150 155 160 165
ggc cgg ttt ggg ggc tct ctt ccc gat cag atc acg gtg cat ctc gat 643
Gly Arg Phe Gly Gly Ser Leu Pro Asp Gln Ile Thr Val His Leu Asp
170 175 180
gct ggg tat gac tcg aag aaa acc cgc agg cta ctc agc gaa ttt ggt 691
Ala Gly Tyr Asp Ser Lys Lys Thr Arg Arg Leu Leu Ser Glu Phe Gly
185 190 195
tat agc tgg gtg atc agc att aaa ggt gag ccg ctg cag gct ggg act 739
Tyr Ser Trp Val Ile Ser Ile Lys Gly Glu Pro Leu Gln Ala Gly Thr
200 205 210
cgg tgg gtg gtg gag cgt act aac tct tgg cat aac cgg ggt ttt aag 787
Arg Trp Val Val Glu Arg Thr Asn Ser Trp His Asn Arg Gly Phe Lys
215 220 225
aaa ctt agt atc tgc acc gaa cgt tgt acc cgg gtt gtg gaa gcg ttt 835
Lys Leu Ser Ile Cys Thr Glu Arg Cys Thr Arg Val Val Glu Ala Phe
230 235 240 245
atc gct tta gcc aac gcg gtg att att ctg cgt cgg ctt atc aaa cag 883
Ile Ala Leu Ala Asn Ala Val Ile Ile Leu Arg Arg Leu Ile Lys Gln
250 255 260
gcc tgg act agt tac cgc tgg gac acc cga ccg ggc cac aga cct 928
Ala Trp Thr Ser Tyr Arg Trp Asp Thr Arg Pro Gly His Arg Pro
265 270 275
taatctatcc gcgcaatctc taaggagaaa 958
<210> 86
<211> 276
<212> PRT
<213> Corynebacterium glutamicum
<400> 86
Val Pro Ala Leu Pro Ser Ser Ile Ile Asp Pro Leu Trp Arg Gln Phe
1 5 10 15
Ser Ala Leu Ile Pro Pro Val Ile Ile Thr His Pro Leu Gly Cys His
20 25 30
Arg Ala Arg Ile Ala Asp Arg Ile Ile Val Asp Lys Leu Ile Ala Val
35 40 45
Leu Val Leu Gly Val Ser Tyr Ile Lys Ile Ser Asp Ser Thr Cys Ser
50 55 60
Ala Thr Thr Ile Arg Thr Arg Arg Asp Glu Trp Ile Thr Ala Gly Ile
65 70 75 80
Phe Lys Asn Leu Glu Gln Ile Cys Leu Glu Ser Tyr Asp Arg Phe Ile
85 90 95
Gly Leu Asp Leu Glu Asn Leu Asn Val Asp Gly Cys Ile Val Lys Ala
100 105 110
Pro Cys Gly Gly Glu Val Ala Gly Arg Phe Pro Val Asp Arg Glu Lys
115 120 125
Gly Thr Lys Arg Ser Leu Met Val Asp Gly His Gly Ile Pro Ile Gly
130 135 140
Cys Val Val Ala Gly Ala Asn Arg His Asp Leu Pro Leu Leu Ala Ala
145 150 155 160
Thr Leu Asp Thr Leu Gly Arg Phe Gly Gly Ser Leu Pro Asp Gln Ile
165 170 175
Thr Val His Leu Asp Ala Gly Tyr Asp Ser Lys Lys Thr Arg Arg Leu
180 185 190
Leu Ser Glu Phe Gly Tyr Ser Trp Val Ile Ser Ile Lys Gly Glu Pro
195 200 205
Leu Gln Ala Gly Thr Arg Trp Val Val Glu Arg Thr Asn Ser Trp His
210 215 220
Asn Arg Gly Phe Lys Lys Leu Ser Ile Cys Thr Glu Arg Cys Thr Arg
225 230 235 240
Val Val Glu Ala Phe Ile Ala Leu Ala Asn Ala Val Ile Ile Leu Arg
245 250 255
Arg Leu Ile Lys Gln Ala Trp Thr Ser Tyr Arg Trp Asp Thr Arg Pro
260 265 270
Gly His Arg Pro
275
<210> 87
<211> 754
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(724)
<223> RXA03098
<400> 87
gaccgttttg tcgatcgcac cgctgctggc tcgcaccatc aacgagatct tcgaaaacgg 60
ttccgtcacc accctcttcg agggcgaggc ctaaacaccc atg ccc acc acg gac 115
Met Pro Thr Thr Asp
1 5
gtc ttc aac cgc gtc cgg ttg gca ttg gaa cct cta gct gat ccc gca 163
Val Phe Asn Arg Val Arg Leu Ala Leu Glu Pro Leu Ala Asp Pro Ala
10 15 20
cgt gcc acc gga atg gca agc tac atg cgg gat cag ttt tct ttt ctc 211
Arg Ala Thr Gly Met Ala Ser Tyr Met Arg Asp Gln Phe Ser Phe Leu
25 30 35
ggc atc cca tcc acc ccc aga aaa gaa gcc tgc aaa ccc gtg ctg tcc 259
Gly Ile Pro Ser Thr Pro Arg Lys Glu Ala Cys Lys Pro Val Leu Ser
40 45 50
gcg cta aaa gag ttg gac act gac ttt gtc tca gac tgc ttt ggc gca 307
Ala Leu Lys Glu Leu Asp Thr Asp Phe Val Ser Asp Cys Phe Gly Ala
55 60 65
gct gaa cgg gaa tac cag tat gtc gcc tgc gat cac atc aat cgc gtc 355
Ala Glu Arg Glu Tyr Gln Tyr Val Ala Cys Asp His Ile Asn Arg Val
70 75 80 85
ggc atc acc gat cta ggt ttt gcc aaa gca tta gtg cag acc aaa tcc 403
Gly Ile Thr Asp Leu Gly Phe Ala Lys Ala Leu Val Gln Thr Lys Ser
90 95 100
tgg tgg gac acc gtc gat tcc cta gca aaa ccg atc ggc gcc aaa cac 451
Trp Trp Asp Thr Val Asp Ser Leu Ala Lys Pro Ile Gly Ala Lys His
105 110 115
gat gat gat ctg atg aaa acg tgg gcg ctt gat gag gac ttc tgg gtg 499
Asp Asp Asp Leu Met Lys Thr Trp Ala Leu Asp Glu Asp Phe Trp Val
120 125 130
cgc cgc atc gcg atc atc cac caa ctg ggc cgc aag aaa aac acc gac 547
Arg Arg Ile Ala Ile Ile His Gln Leu Gly Arg Lys Lys Asn Thr Asp
135 140 145
gct gcc ctg ctg gcc tgg atc atc gag cag aac ctc ggc tcc agc gag 595
Ala Ala Leu Leu Ala Trp Ile Ile Glu Gln Asn Leu Gly Ser Ser Glu
150 155 160 165
ttc ttc atc aac aaa gcg atc ggc tgg gca ctg cgg gat ttc gcc cgc 643
Phe Phe Ile Asn Lys Ala Ile Gly Trp Ala Leu Arg Asp Phe Ala Arg
170 175 180
cac gac ccc agc tgg gtc cgg gct ttt gtc gac gcc acg gac ctt tcc 691
His Asp Pro Ser Trp Val Arg Ala Phe Val Asp Ala Thr Asp Leu Ser
185 190 195
cca ctg agc cgg cga gaa gcc ctg aag aat att tagccctcag gcatcatctg 744
Pro Leu Ser Arg Arg Glu Ala Leu Lys Asn Ile
200 205
agcgagtgcc 754
<210> 88
<211> 208
<212> PRT
<213> Corynebacterium glutamicum
<400> 88
Met Pro Thr Thr Asp Val Phe Asn Arg Val Arg Leu Ala Leu Glu Pro
1 5 10 15
Leu Ala Asp Pro Ala Arg Ala Thr Gly Met Ala Ser Tyr Met Arg Asp
20 25 30
Gln Phe Ser Phe Leu Gly Ile Pro Ser Thr Pro Arg Lys Glu Ala Cys
35 40 45
Lys Pro Val Leu Ser Ala Leu Lys Glu Leu Asp Thr Asp Phe Val Ser
50 55 60
Asp Cys Phe Gly Ala Ala Glu Arg Glu Tyr Gln Tyr Val Ala Cys Asp
65 70 75 80
His Ile Asn Arg Val Gly Ile Thr Asp Leu Gly Phe Ala Lys Ala Leu
85 90 95
Val Gln Thr Lys Ser Trp Trp Asp Thr Val Asp Ser Leu Ala Lys Pro
100 105 110
Ile Gly Ala Lys His Asp Asp Asp Leu Met Lys Thr Trp Ala Leu Asp
115 120 125
Glu Asp Phe Trp Val Arg Arg Ile Ala Ile Ile His Gln Leu Gly Arg
130 135 140
Lys Lys Asn Thr Asp Ala Ala Leu Leu Ala Trp Ile Ile Glu Gln Asn
145 150 155 160
Leu Gly Ser Ser Glu Phe Phe Ile Asn Lys Ala Ile Gly Trp Ala Leu
165 170 175
Arg Asp Phe Ala Arg His Asp Pro Ser Trp Val Arg Ala Phe Val Asp
180 185 190
Ala Thr Asp Leu Ser Pro Leu Ser Arg Arg Glu Ala Leu Lys Asn Ile
195 200 205
<210> 89
<211> 562
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(532)
<223> RXA03206
<400> 89
gaatccctgc acaacggcgc cattgcggcg ttggtggatc tcatccgcca cggattggtg 60
ttgcccgctg atcttctcga ttcttaaata aggactgatt gtg aaa gcc gtt tta 115
Val Lys Ala Val Leu
1 5
acc cgt gtg agt tcc gcc agc gtc agc gtg gat gat gaa att gtt gga 163
Thr Arg Val Ser Ser Ala Ser Val Ser Val Asp Asp Glu Ile Val Gly
10 15 20
gcc atc gat tgc ccc gac acc gga ggc att ttg gcg ctg gtt gga gtc 211
Ala Ile Asp Cys Pro Asp Thr Gly Gly Ile Leu Ala Leu Val Gly Val
25 30 35
ggc gct gct gat agc gac gac gcc tgg gaa acc atg gtg cga aaa att 259
Gly Ala Ala Asp Ser Asp Asp Ala Trp Glu Thr Met Val Arg Lys Ile
40 45 50
gct gag ctg cgc atc ttg gat ggc gaa caa tcc gtc agt gat gtc aat 307
Ala Glu Leu Arg Ile Leu Asp Gly Glu Gln Ser Val Ser Asp Val Asn
55 60 65
gct ccc gta ctg ctt gtt agc caa ttc acc ctg cat ggt cgc acc gca 355
Ala Pro Val Leu Leu Val Ser Gln Phe Thr Leu His Gly Arg Thr Ala
70 75 80 85
aaa ggc cgg cgc cca tcg tgg tct gat gca gca cct ggt gag gtg gct 403
Lys Gly Arg Arg Pro Ser Trp Ser Asp Ala Ala Pro Gly Glu Val Ala
90 95 100
gag ccg gtg att gaa aag att gca caa ggt tta cgt gag cgc gga atc 451
Glu Pro Val Ile Glu Lys Ile Ala Gln Gly Leu Arg Glu Arg Gly Ile
105 110 115
acc gtg gaa caa gga cga ttc ggc gca atg atg aag gtc aca tcg gtt 499
Thr Val Glu Gln Gly Arg Phe Gly Ala Met Met Lys Val Thr Ser Val
120 125 130
aac gaa ggc ccc ttc acc gtt ttg gtc gag tgc tagccagtca atcctaagag 552
Asn Glu Gly Pro Phe Thr Val Leu Val Glu Cys
135 140
cttgaaacgc 562
<210> 90
<211> 144
<212> PRT
<213> Corynebacterium glutamicum
<400> 90
Val Lys Ala Val Leu Thr Arg Val Ser Ser Ala Ser Val Ser Val Asp
1 5 10 15
Asp Glu Ile Val Gly Ala Ile Asp Cys Pro Asp Thr Gly Gly Ile Leu
20 25 30
Ala Leu Val Gly Val Gly Ala Ala Asp Ser Asp Asp Ala Trp Glu Thr
35 40 45
Met Val Arg Lys Ile Ala Glu Leu Arg Ile Leu Asp Gly Glu Gln Ser
50 55 60
Val Ser Asp Val Asn Ala Pro Val Leu Leu Val Ser Gln Phe Thr Leu
65 70 75 80
His Gly Arg Thr Ala Lys Gly Arg Arg Pro Ser Trp Ser Asp Ala Ala
85 90 95
Pro Gly Glu Val Ala Glu Pro Val Ile Glu Lys Ile Ala Gln Gly Leu
100 105 110
Arg Glu Arg Gly Ile Thr Val Glu Gln Gly Arg Phe Gly Ala Met Met
115 120 125
Lys Val Thr Ser Val Asn Glu Gly Pro Phe Thr Val Leu Val Glu Cys
130 135 140
<210> 91
<211> 607
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(577)
<223> RXA03260
<400> 91
cagatggctg gcagggatca gctgaccacg ttgctcaacc agcgtggtgt caaagtttct 60
actgggactg tgggatcaat tatgaacgaa tgaggagtgc gtg tca gac gaa tgc 115
Val Ser Asp Glu Cys
1 5
ggg cct gga aga aca cca cgg tca gtg acc ctt tct gcc cgg acc gag 163
Gly Pro Gly Arg Thr Pro Arg Ser Val Thr Leu Ser Ala Arg Thr Glu
10 15 20
cat att aaa aat cat atg ctc gat agc cac ggg aaa cga gac ttt acc 211
His Ile Lys Asn His Met Leu Asp Ser His Gly Lys Arg Asp Phe Thr
25 30 35
gct acc gtg cct ggg acc agg ctc gtt ggt gac att acg tac tta aag 259
Ala Thr Val Pro Gly Thr Arg Leu Val Gly Asp Ile Thr Tyr Leu Lys
40 45 50
acg ggt tcc ggg tgg ctg tat gtg gct acc gtg atc gat ttg gct acg 307
Thr Gly Ser Gly Trp Leu Tyr Val Ala Thr Val Ile Asp Leu Ala Thr
55 60 65
cgg atg gtg gtg ggg tgg tct atg gat tct aat atg cgc aca ccg ttg 355
Arg Met Val Val Gly Trp Ser Met Asp Ser Asn Met Arg Thr Pro Leu
70 75 80 85
gtg atc aat gcg ctg gct atg gcg cgt gat cat ggg tgt ctt cat cct 403
Val Ile Asn Ala Leu Ala Met Ala Arg Asp His Gly Cys Leu His Pro
90 95 100
gaa ggc gca att ttt cac tcc gat aga gga tcg caa tac acc tcc gag 451
Glu Gly Ala Ile Phe His Ser Asp Arg Gly Ser Gln Tyr Thr Ser Glu
105 110 115
cag ttc cag aca tgg tgc gcc ggc aac aag atc acc caa tcc atg gga 499
Gln Phe Gln Thr Trp Cys Ala Gly Asn Lys Ile Thr Gln Ser Met Gly
120 125 130
ttg acc ggg gtg tgt tgg gat aac gga agt cgc gga gaa ttt ttt ctc 547
Leu Thr Gly Val Cys Trp Asp Asn Gly Ser Arg Gly Glu Phe Phe Leu
135 140 145
aca ttt gaa gac cga aat gta tca cca cta tgattttgag aatcacctgt 597
Thr Phe Glu Asp Arg Asn Val Ser Pro Leu
150 155
cggaccgaac 607
<210> 92
<211> 159
<212> PRT
<213> Corynebacterium glutamicum
<400> 92
Val Ser Asp Glu Cys Gly Pro Gly Arg Thr Pro Arg Ser Val Thr Leu
1 5 10 15
Ser Ala Arg Thr Glu His Ile Lys Asn His Met Leu Asp Ser His Gly
20 25 30
Lys Arg Asp Phe Thr Ala Thr Val Pro Gly Thr Arg Leu Val Gly Asp
35 40 45
Ile Thr Tyr Leu Lys Thr Gly Ser Gly Trp Leu Tyr Val Ala Thr Val
50 55 60
Ile Asp Leu Ala Thr Arg Met Val Val Gly Trp Ser Met Asp Ser Asn
65 70 75 80
Met Arg Thr Pro Leu Val Ile Asn Ala Leu Ala Met Ala Arg Asp His
85 90 95
Gly Cys Leu His Pro Glu Gly Ala Ile Phe His Ser Asp Arg Gly Ser
100 105 110
Gln Tyr Thr Ser Glu Gln Phe Gln Thr Trp Cys Ala Gly Asn Lys Ile
115 120 125
Thr Gln Ser Met Gly Leu Thr Gly Val Cys Trp Asp Asn Gly Ser Arg
130 135 140
Gly Glu Phe Phe Leu Thr Phe Glu Asp Arg Asn Val Ser Pro Leu
145 150 155
<210> 93
<211> 1969
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1939)
<223> RXA03394
<400> 93
ctgcaaagcg acgcagggag cgtaaggcga gtggcgcggg gaagcgtcga taggcaattt 60
ttaacccctg ataccccttt ccggccgggc ataaattaag gtg gta cgc atg acg 115
Val Val Arg Met Thr
1 5
aag aac gtg ctc gta tct gtt gcc tgg ccg tat gcc aac gga ccc cgt 163
Lys Asn Val Leu Val Ser Val Ala Trp Pro Tyr Ala Asn Gly Pro Arg
10 15 20
cac att gga cat gtg gcg ggg ttt ggt gtc ccc tcc gat gtg ttc gca 211
His Ile Gly His Val Ala Gly Phe Gly Val Pro Ser Asp Val Phe Ala
25 30 35
agg ttc cag cga atg tct ggc aac aac gtg ctc atg gtg tcc ggc acc 259
Arg Phe Gln Arg Met Ser Gly Asn Asn Val Leu Met Val Ser Gly Thr
40 45 50
gat gag cac ggc acg cca ctt ctg gtt caa gca gac aaa gaa ggc gtc 307
Asp Glu His Gly Thr Pro Leu Leu Val Gln Ala Asp Lys Glu Gly Val
55 60 65
acc gtt caa gac cta gcg gat aag tac aac cgc cag atc gtc gaa gac 355
Thr Val Gln Asp Leu Ala Asp Lys Tyr Asn Arg Gln Ile Val Glu Asp
70 75 80 85
ctc acc ggc ctg ggc ctg tcc tat gac ctt ttc acc cgc acc acc acc 403
Leu Thr Gly Leu Gly Leu Ser Tyr Asp Leu Phe Thr Arg Thr Thr Thr
90 95 100
tcc aac cac tac gca gta gtg cag gaa ctg ttc cgt ggt ctg tac gac 451
Ser Asn His Tyr Ala Val Val Gln Glu Leu Phe Arg Gly Leu Tyr Asp
105 110 115
aac ggt tac atg atc aag gaa acc acc ctc ggt gcg att tcc cca tcc 499
Asn Gly Tyr Met Ile Lys Glu Thr Thr Leu Gly Ala Ile Ser Pro Ser
120 125 130
act ggc cgt acc ctg cca gac cgc tac att gaa ggc acc tgc cca atc 547
Thr Gly Arg Thr Leu Pro Asp Arg Tyr Ile Glu Gly Thr Cys Pro Ile
135 140 145
tgt ggc acc gac ggc gct cgt ggc gac cag tgc gac aac tgc gga aac 595
Cys Gly Thr Asp Gly Ala Arg Gly Asp Gln Cys Asp Asn Cys Gly Asn
150 155 160 165
cag ctc gat cca gcg gac ctg atc aac ccg gtg tcc aag atc aac ggc 643
Gln Leu Asp Pro Ala Asp Leu Ile Asn Pro Val Ser Lys Ile Asn Gly
170 175 180
gaa acc cca gag ttc gtt gag acc gaa cac ttc ctg ctc gac ctg cca 691
Glu Thr Pro Glu Phe Val Glu Thr Glu His Phe Leu Leu Asp Leu Pro
185 190 195
gca ctg gct gaa gca cta acc gag tgg ctg aag gga cgc gaa gac tgg 739
Ala Leu Ala Glu Ala Leu Thr Glu Trp Leu Lys Gly Arg Glu Asp Trp
200 205 210
cgt cca aac gtg ttg aag ttc tcg ctc aac ctg ctg gac gat atc cgc 787
Arg Pro Asn Val Leu Lys Phe Ser Leu Asn Leu Leu Asp Asp Ile Arg
215 220 225
cca cgc gca atg tcg cgc gat atc gac tgg ggc atc cca atc cca gtt 835
Pro Arg Ala Met Ser Arg Asp Ile Asp Trp Gly Ile Pro Ile Pro Val
230 235 240 245
gaa gga tgg caa gac aac aac gcc aag aag ctc tac gtc tgg ttc gac 883
Glu Gly Trp Gln Asp Asn Asn Ala Lys Lys Leu Tyr Val Trp Phe Asp
250 255 260
gct gtc gtg ggc tac ttg tcc gca tcc atc gaa tgg gcc tac cgc tcc 931
Ala Val Val Gly Tyr Leu Ser Ala Ser Ile Glu Trp Ala Tyr Arg Ser
265 270 275
ggc gac cca gaa gca tgg cgc acc ttc tgg aat gat cca gaa acc aag 979
Gly Asp Pro Glu Ala Trp Arg Thr Phe Trp Asn Asp Pro Glu Thr Lys
280 285 290
tcc tac tac ttc atg ggc aaa gac aac atc acc ttc cac tcc cag atc 1027
Ser Tyr Tyr Phe Met Gly Lys Asp Asn Ile Thr Phe His Ser Gln Ile
295 300 305
tgg cca gcg gag ctt ctc ggc tac gca ggc aag ggc tcc cgc ggt gga 1075
Trp Pro Ala Glu Leu Leu Gly Tyr Ala Gly Lys Gly Ser Arg Gly Gly
310 315 320 325
gaa atc ggt gac ctg ggt gtt ctg aac ctg cct act gag gtt gtt tcc 1123
Glu Ile Gly Asp Leu Gly Val Leu Asn Leu Pro Thr Glu Val Val Ser
330 335 340
tct gag ttc ctg act atg tct gga tcc aag ttc tcc tca tcc aag ggc 1171
Ser Glu Phe Leu Thr Met Ser Gly Ser Lys Phe Ser Ser Ser Lys Gly
345 350 355
gtt gtc atc tac gtg aag gac ttc ctc aag gag ttc ggc cca gat gcg 1219
Val Val Ile Tyr Val Lys Asp Phe Leu Lys Glu Phe Gly Pro Asp Ala
360 365 370
ctg cga tac ttc atc gct gtc gca ggc cca gaa aac aac gac acc gac 1267
Leu Arg Tyr Phe Ile Ala Val Ala Gly Pro Glu Asn Asn Asp Thr Asp
375 380 385
ttc acc tgg gat gaa ttt gtc cgc cgc gta aat aac gag ctg gca aac 1315
Phe Thr Trp Asp Glu Phe Val Arg Arg Val Asn Asn Glu Leu Ala Asn
390 395 400 405
ggc tgg ggc aac ctg gtc aac cgc act gta tcc atg gcg cac aag aac 1363
Gly Trp Gly Asn Leu Val Asn Arg Thr Val Ser Met Ala His Lys Asn
410 415 420
ttc ggt gaa gta cca gta cct ggc gca ctg gaa gaa tct gac aag aag 1411
Phe Gly Glu Val Pro Val Pro Gly Ala Leu Glu Glu Ser Asp Lys Lys
425 430 435
atc ctt gat ctt gct acc gct gcc ttt gaa tcc gtt gct gcg aac ctg 1459
Ile Leu Asp Leu Ala Thr Ala Ala Phe Glu Ser Val Ala Ala Asn Leu
440 445 450
gat cag tcc aag ttc aag gcc ggt atc tct gaa atc atg cac gtt gtc 1507
Asp Gln Ser Lys Phe Lys Ala Gly Ile Ser Glu Ile Met His Val Val
455 460 465
ggt gag gcc aac gcc tac atc gca gag caa gaa cca tgg aag ctt gcc 1555
Gly Glu Ala Asn Ala Tyr Ile Ala Glu Gln Glu Pro Trp Lys Leu Ala
470 475 480 485
aag gat gac acc aag cgc gag cgt ctt gcc acc gtg ctg tgg act gcg 1603
Lys Asp Asp Thr Lys Arg Glu Arg Leu Ala Thr Val Leu Trp Thr Ala
490 495 500
ctg cag gtt gtt tct gac tgc aac acc atg ctg acc cca tac ctg cca 1651
Leu Gln Val Val Ser Asp Cys Asn Thr Met Leu Thr Pro Tyr Leu Pro
505 510 515
cac acc gcc caa aag gtg cat gag acc ttg ggc cgt gat gga atc tgg 1699
His Thr Ala Gln Lys Val His Glu Thr Leu Gly Arg Asp Gly Ile Trp
520 525 530
gct gca aca cca cag atc gtg gaa gtc acc aac gaa tca cca cgc cag 1747
Ala Ala Thr Pro Gln Ile Val Glu Val Thr Asn Glu Ser Pro Arg Gln
535 540 545
cca atc ggc gtg ggg cta cca gat cca gag cac acc tac cca gta atc 1795
Pro Ile Gly Val Gly Leu Pro Asp Pro Glu His Thr Tyr Pro Val Ile
550 555 560 565
atg ggc gac tac aag acc cag ctg gct aag tgg cag cgc atc gac gtt 1843
Met Gly Asp Tyr Lys Thr Gln Leu Ala Lys Trp Gln Arg Ile Asp Val
570 575 580
gtg cca ggc acc acc ttg gag aag cca gca ccg ctg att gct aag ctc 1891
Val Pro Gly Thr Thr Leu Glu Lys Pro Ala Pro Leu Ile Ala Lys Leu
585 590 595
gat cca gaa ctt ggt gaa acc ggc cca gaa tgg gca cca gtg cag aac 1939
Asp Pro Glu Leu Gly Glu Thr Gly Pro Glu Trp Ala Pro Val Gln Asn
600 605 610
taaagcatct ttagcatgaa ccgagcaggt 1969
<210> 94
<211> 613
<212> PRT
<213> Corynebacterium glutamicum
<400> 94
Val Val Arg Met Thr Lys Asn Val Leu Val Ser Val Ala Trp Pro Tyr
1 5 10 15
Ala Asn Gly Pro Arg His Ile Gly His Val Ala Gly Phe Gly Val Pro
20 25 30
Ser Asp Val Phe Ala Arg Phe Gln Arg Met Ser Gly Asn Asn Val Leu
35 40 45
Met Val Ser Gly Thr Asp Glu His Gly Thr Pro Leu Leu Val Gln Ala
50 55 60
Asp Lys Glu Gly Val Thr Val Gln Asp Leu Ala Asp Lys Tyr Asn Arg
65 70 75 80
Gln Ile Val Glu Asp Leu Thr Gly Leu Gly Leu Ser Tyr Asp Leu Phe
85 90 95
Thr Arg Thr Thr Thr Ser Asn His Tyr Ala Val Val Gln Glu Leu Phe
100 105 110
Arg Gly Leu Tyr Asp Asn Gly Tyr Met Ile Lys Glu Thr Thr Leu Gly
115 120 125
Ala Ile Ser Pro Ser Thr Gly Arg Thr Leu Pro Asp Arg Tyr Ile Glu
130 135 140
Gly Thr Cys Pro Ile Cys Gly Thr Asp Gly Ala Arg Gly Asp Gln Cys
145 150 155 160
Asp Asn Cys Gly Asn Gln Leu Asp Pro Ala Asp Leu Ile Asn Pro Val
165 170 175
Ser Lys Ile Asn Gly Glu Thr Pro Glu Phe Val Glu Thr Glu His Phe
180 185 190
Leu Leu Asp Leu Pro Ala Leu Ala Glu Ala Leu Thr Glu Trp Leu Lys
195 200 205
Gly Arg Glu Asp Trp Arg Pro Asn Val Leu Lys Phe Ser Leu Asn Leu
210 215 220
Leu Asp Asp Ile Arg Pro Arg Ala Met Ser Arg Asp Ile Asp Trp Gly
225 230 235 240
Ile Pro Ile Pro Val Glu Gly Trp Gln Asp Asn Asn Ala Lys Lys Leu
245 250 255
Tyr Val Trp Phe Asp Ala Val Val Gly Tyr Leu Ser Ala Ser Ile Glu
260 265 270
Trp Ala Tyr Arg Ser Gly Asp Pro Glu Ala Trp Arg Thr Phe Trp Asn
275 280 285
Asp Pro Glu Thr Lys Ser Tyr Tyr Phe Met Gly Lys Asp Asn Ile Thr
290 295 300
Phe His Ser Gln Ile Trp Pro Ala Glu Leu Leu Gly Tyr Ala Gly Lys
305 310 315 320
Gly Ser Arg Gly Gly Glu Ile Gly Asp Leu Gly Val Leu Asn Leu Pro
325 330 335
Thr Glu Val Val Ser Ser Glu Phe Leu Thr Met Ser Gly Ser Lys Phe
340 345 350
Ser Ser Ser Lys Gly Val Val Ile Tyr Val Lys Asp Phe Leu Lys Glu
355 360 365
Phe Gly Pro Asp Ala Leu Arg Tyr Phe Ile Ala Val Ala Gly Pro Glu
370 375 380
Asn Asn Asp Thr Asp Phe Thr Trp Asp Glu Phe Val Arg Arg Val Asn
385 390 395 400
Asn Glu Leu Ala Asn Gly Trp Gly Asn Leu Val Asn Arg Thr Val Ser
405 410 415
Met Ala His Lys Asn Phe Gly Glu Val Pro Val Pro Gly Ala Leu Glu
420 425 430
Glu Ser Asp Lys Lys Ile Leu Asp Leu Ala Thr Ala Ala Phe Glu Ser
435 440 445
Val Ala Ala Asn Leu Asp Gln Ser Lys Phe Lys Ala Gly Ile Ser Glu
450 455 460
Ile Met His Val Val Gly Glu Ala Asn Ala Tyr Ile Ala Glu Gln Glu
465 470 475 480
Pro Trp Lys Leu Ala Lys Asp Asp Thr Lys Arg Glu Arg Leu Ala Thr
485 490 495
Val Leu Trp Thr Ala Leu Gln Val Val Ser Asp Cys Asn Thr Met Leu
500 505 510
Thr Pro Tyr Leu Pro His Thr Ala Gln Lys Val His Glu Thr Leu Gly
515 520 525
Arg Asp Gly Ile Trp Ala Ala Thr Pro Gln Ile Val Glu Val Thr Asn
530 535 540
Glu Ser Pro Arg Gln Pro Ile Gly Val Gly Leu Pro Asp Pro Glu His
545 550 555 560
Thr Tyr Pro Val Ile Met Gly Asp Tyr Lys Thr Gln Leu Ala Lys Trp
565 570 575
Gln Arg Ile Asp Val Val Pro Gly Thr Thr Leu Glu Lys Pro Ala Pro
580 585 590
Leu Ile Ala Lys Leu Asp Pro Glu Leu Gly Glu Thr Gly Pro Glu Trp
595 600 605
Ala Pro Val Gln Asn
610
<210> 95
<211> 3016
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(2986)
<223> RXA03674
<400> 95
tccccgcagc ccaccaccgt gggctgcggg gtgtggcgtt tttgccacaa agtggaccgt 60
attcgcaaat actttgttaa gacgcgttaa tctttaacct atg tct gaa tca ggt 115
Met Ser Glu Ser Gly
1 5
gcg cta agt tct act gac tct cta tcc ccg ggt gtc acc att gaa gtc 163
Ala Leu Ser Ser Thr Asp Ser Leu Ser Pro Gly Val Thr Ile Glu Val
10 15 20
cga gat gaa att tgg ctg gtt act cac gtt act cgc tcc aca gat ggt 211
Arg Asp Glu Ile Trp Leu Val Thr His Val Thr Arg Ser Thr Asp Gly
25 30 35
ttt agg gtt aaa gct cgt ggt ctc tct gat tat gtg cgg gac cac gaa 259
Phe Arg Val Lys Ala Arg Gly Leu Ser Asp Tyr Val Arg Asp His Glu
40 45 50
gct acg ttc ttc acc gca ctt gat aaa gat ttg aag gtc att gac cct 307
Ala Thr Phe Phe Thr Ala Leu Asp Lys Asp Leu Lys Val Ile Asp Pro
55 60 65
acc cag gtc acc gtc agt ctt gat gat tcc tcc aat tac cgt cgc acc 355
Thr Gln Val Thr Val Ser Leu Asp Asp Ser Ser Asn Tyr Arg Arg Thr
70 75 80 85
cgc ctg tgg ttg gag gcc acc atg cgt aaa act ccg gta ccg ctc tat 403
Arg Leu Trp Leu Glu Ala Thr Met Arg Lys Thr Pro Val Pro Leu Tyr
90 95 100
caa gag tca ctt tcc gtg gca gat caa atg ctc gcc gat cca ctg gag 451
Gln Glu Ser Leu Ser Val Ala Asp Gln Met Leu Ala Asp Pro Leu Glu
105 110 115
tac caa tta gca gcc gtg cgc aaa acc ctc tct agt gct aac ttg cgc 499
Tyr Gln Leu Ala Ala Val Arg Lys Thr Leu Ser Ser Ala Asn Leu Arg
120 125 130
ccc cgc gtg ctt att gct gat gcc gtg gga ctt ggc aaa acc cta gaa 547
Pro Arg Val Leu Ile Ala Asp Ala Val Gly Leu Gly Lys Thr Leu Glu
135 140 145
atg ggc atg atc ttg gcg gaa ctt atc cgc cgt ggc cgt ggt gag cgc 595
Met Gly Met Ile Leu Ala Glu Leu Ile Arg Arg Gly Arg Gly Glu Arg
150 155 160 165
att ttg gta gtc acc ccg cgc cac att atg gag cag ttc cag cag gaa 643
Ile Leu Val Val Thr Pro Arg His Ile Met Glu Gln Phe Gln Gln Glu
170 175 180
atg tgg acc cgt ttt gcc atc ccg ctc gtt cgt cta gat tcc gtg ggc 691
Met Trp Thr Arg Phe Ala Ile Pro Leu Val Arg Leu Asp Ser Val Gly
185 190 195
atc cag caa gtg cgc caa aaa ttg cca gca tca cgc aac cct ttt act 739
Ile Gln Gln Val Arg Gln Lys Leu Pro Ala Ser Arg Asn Pro Phe Thr
200 205 210
tat ttc ccg cgc gtg att gtc tct atg gat act ttg aaa tct ccg aag 787
Tyr Phe Pro Arg Val Ile Val Ser Met Asp Thr Leu Lys Ser Pro Lys
215 220 225
tac cgc gcg caa cta gaa aag gtg cac tgg gat gcg gtg gtt ata gat 835
Tyr Arg Ala Gln Leu Glu Lys Val His Trp Asp Ala Val Val Ile Asp
230 235 240 245
gaa atc cac aat gca acc aat gct ggc acc caa aat aat gag cta gcc 883
Glu Ile His Asn Ala Thr Asn Ala Gly Thr Gln Asn Asn Glu Leu Ala
250 255 260
cgc aca ctt gcg cct act gcc gag gct ctt att ttg gcc tct gcc acc 931
Arg Thr Leu Ala Pro Thr Ala Glu Ala Leu Ile Leu Ala Ser Ala Thr
265 270 275
ccg cac aat ggt gat cca gaa tcc ttt aag gag atc ttg cgt ttg ctt 979
Pro His Asn Gly Asp Pro Glu Ser Phe Lys Glu Ile Leu Arg Leu Leu
280 285 290
gat ccc acc gct gtg atg cct gat ggc acc att gat gcc gaa gct gca 1027
Asp Pro Thr Ala Val Met Pro Asp Gly Thr Ile Asp Ala Glu Ala Ala
295 300 305
cag cgt ctg atc att cgt cgc cat cgc aat agc cct gag gtt tca ggt 1075
Gln Arg Leu Ile Ile Arg Arg His Arg Asn Ser Pro Glu Val Ser Gly
310 315 320 325
ttt gtg ggc gaa aaa tgg gct cca cgc aat gag cct cag aac ttc ctg 1123
Phe Val Gly Glu Lys Trp Ala Pro Arg Asn Glu Pro Gln Asn Phe Leu
330 335 340
gtc gct gcg tca aaa gaa gaa aac ggc gtt gct gca gaa ctc aac cat 1171
Val Ala Ala Ser Lys Glu Glu Asn Gly Val Ala Ala Glu Leu Asn His
345 350 355
gtg tgg att tca cca ggt gcg agc aat ccg atc aag gat cgc ctc ttc 1219
Val Trp Ile Ser Pro Gly Ala Ser Asn Pro Ile Lys Asp Arg Leu Phe
360 365 370
ccc tgg aca ttg gtg aag gct ttt ctc tcc tcc cct gca gcc ttg ggc 1267
Pro Trp Thr Leu Val Lys Ala Phe Leu Ser Ser Pro Ala Ala Leu Gly
375 380 385
gaa aca gtg tcc aat cgc ctc aaa aag gcc tct gca cca gaa gaa aaa 1315
Glu Thr Val Ser Asn Arg Leu Lys Lys Ala Ser Ala Pro Glu Glu Lys
390 395 400 405
cgc gcc cta gaa acc ctt tca caa ctt aat tct gcg atc acc ccg cag 1363
Arg Ala Leu Glu Thr Leu Ser Gln Leu Asn Ser Ala Ile Thr Pro Gln
410 415 420
acc tca cag aag tac caa tct cta ctg agc tac ctc ggt gac atc gga 1411
Thr Ser Gln Lys Tyr Gln Ser Leu Leu Ser Tyr Leu Gly Asp Ile Gly
425 430 435
gtg aag aag aac tcc gat acc cgc gtg gtg att ttc tct gag cgt gtc 1459
Val Lys Lys Asn Ser Asp Thr Arg Val Val Ile Phe Ser Glu Arg Val
440 445 450
gct act ttg cac tgg ctg cag gaa aac ctc atc cgt gat ctc aag atg 1507
Ala Thr Leu His Trp Leu Gln Glu Asn Leu Ile Arg Asp Leu Lys Met
455 460 465
cca ccc aac tct att gct gtt atg cac ggc ggt ctc ccc gac cag gag 1555
Pro Pro Asn Ser Ile Ala Val Met His Gly Gly Leu Pro Asp Gln Glu
470 475 480 485
caa atg cgc ctg gtg gat gag ttt aaa aag acg gat tct ccc atc cgc 1603
Gln Met Arg Leu Val Asp Glu Phe Lys Lys Thr Asp Ser Pro Ile Arg
490 495 500
atc atg atc acc ggc gat gtt gcc tca gaa ggt gtg aac ctg cat act 1651
Ile Met Ile Thr Gly Asp Val Ala Ser Glu Gly Val Asn Leu His Thr
505 510 515
ctc tgc cac aac ttg gtg cac tat gac atc ccg tgg tca ctg atc cgc 1699
Leu Cys His Asn Leu Val His Tyr Asp Ile Pro Trp Ser Leu Ile Arg
520 525 530
att cag cag cgc aat ggc cgt att gat cgt tat ggt caa acc cac aac 1747
Ile Gln Gln Arg Asn Gly Arg Ile Asp Arg Tyr Gly Gln Thr His Asn
535 540 545
cct tcc atc gtt acc ttc ttg ctc gat ccc gcc gag gat tcc aaa gta 1795
Pro Ser Ile Val Thr Phe Leu Leu Asp Pro Ala Glu Asp Ser Lys Val
550 555 560 565
ggt gaa gtc cat gtg ctg gag agg ctc atg gag cgc gaa cat gag gcg 1843
Gly Glu Val His Val Leu Glu Arg Leu Met Glu Arg Glu His Glu Ala
570 575 580
cac tct ttg ctc ggt gat gcc gca tct ctc atg ggc aag cac tct gag 1891
His Ser Leu Leu Gly Asp Ala Ala Ser Leu Met Gly Lys His Ser Glu
585 590 595
cgt ttg gaa gaa gaa acc atc cgc gaa gtc ctg cgc ggt gcc caa aac 1939
Arg Leu Glu Glu Glu Thr Ile Arg Glu Val Leu Arg Gly Ala Gln Asn
600 605 610
ttt aat gat gca gtg gct gat cca gcg gaa gtc cta gaa aac cca gca 1987
Phe Asn Asp Ala Val Ala Asp Pro Ala Glu Val Leu Glu Asn Pro Ala
615 620 625
ggc cta gat gat att gat tgg ttg cta gcc caa atc gcc caa gcc gat 2035
Gly Leu Asp Asp Ile Asp Trp Leu Leu Ala Gln Ile Ala Gln Ala Asp
630 635 640 645
gcc aag gca gaa aca gaa gca gaa gca gaa aca gaa aac caa aca gca 2083
Ala Lys Ala Glu Thr Glu Ala Glu Ala Glu Thr Glu Asn Gln Thr Ala
650 655 660
cca gat gca gct tcc aat agc acg cag cat gca caa cgc cgg ttg tat 2131
Pro Asp Ala Ala Ser Asn Ser Thr Gln His Ala Gln Arg Arg Leu Tyr
665 670 675
gca cag gaa agc tct ttc ctc tat gac tgc ctc ctc gaa ggt ttc aat 2179
Ala Gln Glu Ser Ser Phe Leu Tyr Asp Cys Leu Leu Glu Gly Phe Asn
680 685 690
aac gta ccg gag gat tcc atc aac cgc ggt ggc gtg ggg ttc aaa aaa 2227
Asn Val Pro Glu Asp Ser Ile Asn Arg Gly Gly Val Gly Phe Lys Lys
695 700 705
cac gat aat gac atc gtg gag ctc acc ccc acc gat gat ctg cgc cgt 2275
His Asp Asn Asp Ile Val Glu Leu Thr Pro Thr Asp Asp Leu Arg Arg
710 715 720 725
cgt cta gat ttc ctc ccg cag gat tat gtg gct gcc cgg aaa gtt aag 2323
Arg Leu Asp Phe Leu Pro Gln Asp Tyr Val Ala Ala Arg Lys Val Lys
730 735 740
gaa gat ctc cta cta gct tcc aca ctg atg cgt ggc caa gaa cgc ctc 2371
Glu Asp Leu Leu Leu Ala Ser Thr Leu Met Arg Gly Gln Glu Arg Leu
745 750 755
aac gct gcg cgc act ggt gaa gat ggc agt acc tgg cca agt gcc cac 2419
Asn Ala Ala Arg Thr Gly Glu Asp Gly Ser Thr Trp Pro Ser Ala His
760 765 770
tat cta ggc ccc ctg cac cca gtc act tcg tgg gca gct gac cgc gcg 2467
Tyr Leu Gly Pro Leu His Pro Val Thr Ser Trp Ala Ala Asp Arg Ala
775 780 785
ctg gca acc atg cca cgt tcg gaa att ccg gcg gct agt ggc aaa gtc 2515
Leu Ala Thr Met Pro Arg Ser Glu Ile Pro Ala Ala Ser Gly Lys Val
790 795 800 805
aca gag cca acg gtg ctg ctt atg tcc aca ttg agc aat cgg cgt ggc 2563
Thr Glu Pro Thr Val Leu Leu Met Ser Thr Leu Ser Asn Arg Arg Gly
810 815 820
caa att gtg tct cgt tct ttt gtg gct tct tct ggc ccc ttt gat act 2611
Gln Ile Val Ser Arg Ser Phe Val Ala Ser Ser Gly Pro Phe Asp Thr
825 830 835
gag gtg ctg tcc gat ccc atc caa tgg tta cat tcc ata ggc ctc gat 2659
Glu Val Leu Ser Asp Pro Ile Gln Trp Leu His Ser Ile Gly Leu Asp
840 845 850
gaa acc gcc att aac cca ggt acc gct gca ctc ccc gac gat att gag 2707
Glu Thr Ala Ile Asn Pro Gly Thr Ala Ala Leu Pro Asp Asp Ile Glu
855 860 865
cag ctt att tcc ctt gct gtt cag gcc gcc cgc ggc gag atc cgt cca 2755
Gln Leu Ile Ser Leu Ala Val Gln Ala Ala Arg Gly Glu Ile Arg Pro
870 875 880 885
tta atg atc gcc gcc cgc gct cag gct caa act cgc gtt gag cat tgg 2803
Leu Met Ile Ala Ala Arg Ala Gln Ala Gln Thr Arg Val Glu His Trp
890 895 900
gct aag cga gcc gaa gcc tgg aat aac aaa cga agt ggc gca gcg tcc 2851
Ala Lys Arg Ala Glu Ala Trp Asn Asn Lys Arg Ser Gly Ala Ala Ser
905 910 915
acg tcc cgt acc gcg cga act gca aaa ttg att gag gag cag cag aaa 2899
Thr Ser Arg Thr Ala Arg Thr Ala Lys Leu Ile Glu Glu Gln Gln Lys
920 925 930
ttg agt aat gct ctc gag cca gac cgt gaa ctt att agg cct ttg gcc 2947
Leu Ser Asn Ala Leu Glu Pro Asp Arg Glu Leu Ile Arg Pro Leu Ala
935 940 945
gtc att ctt ccg cag ccc gca act ttg aac acc gag gtt taacacaatg 2996
Val Ile Leu Pro Gln Pro Ala Thr Leu Asn Thr Glu Val
950 955 960
agtgcatttg attcgatcct 3016
<210> 96
<211> 962
<212> PRT
<213> Corynebacterium glutamicum
<400> 96
Met Ser Glu Ser Gly Ala Leu Ser Ser Thr Asp Ser Leu Ser Pro Gly
1 5 10 15
Val Thr Ile Glu Val Arg Asp Glu Ile Trp Leu Val Thr His Val Thr
20 25 30
Arg Ser Thr Asp Gly Phe Arg Val Lys Ala Arg Gly Leu Ser Asp Tyr
35 40 45
Val Arg Asp His Glu Ala Thr Phe Phe Thr Ala Leu Asp Lys Asp Leu
50 55 60
Lys Val Ile Asp Pro Thr Gln Val Thr Val Ser Leu Asp Asp Ser Ser
65 70 75 80
Asn Tyr Arg Arg Thr Arg Leu Trp Leu Glu Ala Thr Met Arg Lys Thr
85 90 95
Pro Val Pro Leu Tyr Gln Glu Ser Leu Ser Val Ala Asp Gln Met Leu
100 105 110
Ala Asp Pro Leu Glu Tyr Gln Leu Ala Ala Val Arg Lys Thr Leu Ser
115 120 125
Ser Ala Asn Leu Arg Pro Arg Val Leu Ile Ala Asp Ala Val Gly Leu
130 135 140
Gly Lys Thr Leu Glu Met Gly Met Ile Leu Ala Glu Leu Ile Arg Arg
145 150 155 160
Gly Arg Gly Glu Arg Ile Leu Val Val Thr Pro Arg His Ile Met Glu
165 170 175
Gln Phe Gln Gln Glu Met Trp Thr Arg Phe Ala Ile Pro Leu Val Arg
180 185 190
Leu Asp Ser Val Gly Ile Gln Gln Val Arg Gln Lys Leu Pro Ala Ser
195 200 205
Arg Asn Pro Phe Thr Tyr Phe Pro Arg Val Ile Val Ser Met Asp Thr
210 215 220
Leu Lys Ser Pro Lys Tyr Arg Ala Gln Leu Glu Lys Val His Trp Asp
225 230 235 240
Ala Val Val Ile Asp Glu Ile His Asn Ala Thr Asn Ala Gly Thr Gln
245 250 255
Asn Asn Glu Leu Ala Arg Thr Leu Ala Pro Thr Ala Glu Ala Leu Ile
260 265 270
Leu Ala Ser Ala Thr Pro His Asn Gly Asp Pro Glu Ser Phe Lys Glu
275 280 285
Ile Leu Arg Leu Leu Asp Pro Thr Ala Val Met Pro Asp Gly Thr Ile
290 295 300
Asp Ala Glu Ala Ala Gln Arg Leu Ile Ile Arg Arg His Arg Asn Ser
305 310 315 320
Pro Glu Val Ser Gly Phe Val Gly Glu Lys Trp Ala Pro Arg Asn Glu
325 330 335
Pro Gln Asn Phe Leu Val Ala Ala Ser Lys Glu Glu Asn Gly Val Ala
340 345 350
Ala Glu Leu Asn His Val Trp Ile Ser Pro Gly Ala Ser Asn Pro Ile
355 360 365
Lys Asp Arg Leu Phe Pro Trp Thr Leu Val Lys Ala Phe Leu Ser Ser
370 375 380
Pro Ala Ala Leu Gly Glu Thr Val Ser Asn Arg Leu Lys Lys Ala Ser
385 390 395 400
Ala Pro Glu Glu Lys Arg Ala Leu Glu Thr Leu Ser Gln Leu Asn Ser
405 410 415
Ala Ile Thr Pro Gln Thr Ser Gln Lys Tyr Gln Ser Leu Leu Ser Tyr
420 425 430
Leu Gly Asp Ile Gly Val Lys Lys Asn Ser Asp Thr Arg Val Val Ile
435 440 445
Phe Ser Glu Arg Val Ala Thr Leu His Trp Leu Gln Glu Asn Leu Ile
450 455 460
Arg Asp Leu Lys Met Pro Pro Asn Ser Ile Ala Val Met His Gly Gly
465 470 475 480
Leu Pro Asp Gln Glu Gln Met Arg Leu Val Asp Glu Phe Lys Lys Thr
485 490 495
Asp Ser Pro Ile Arg Ile Met Ile Thr Gly Asp Val Ala Ser Glu Gly
500 505 510
Val Asn Leu His Thr Leu Cys His Asn Leu Val His Tyr Asp Ile Pro
515 520 525
Trp Ser Leu Ile Arg Ile Gln Gln Arg Asn Gly Arg Ile Asp Arg Tyr
530 535 540
Gly Gln Thr His Asn Pro Ser Ile Val Thr Phe Leu Leu Asp Pro Ala
545 550 555 560
Glu Asp Ser Lys Val Gly Glu Val His Val Leu Glu Arg Leu Met Glu
565 570 575
Arg Glu His Glu Ala His Ser Leu Leu Gly Asp Ala Ala Ser Leu Met
580 585 590
Gly Lys His Ser Glu Arg Leu Glu Glu Glu Thr Ile Arg Glu Val Leu
595 600 605
Arg Gly Ala Gln Asn Phe Asn Asp Ala Val Ala Asp Pro Ala Glu Val
610 615 620
Leu Glu Asn Pro Ala Gly Leu Asp Asp Ile Asp Trp Leu Leu Ala Gln
625 630 635 640
Ile Ala Gln Ala Asp Ala Lys Ala Glu Thr Glu Ala Glu Ala Glu Thr
645 650 655
Glu Asn Gln Thr Ala Pro Asp Ala Ala Ser Asn Ser Thr Gln His Ala
660 665 670
Gln Arg Arg Leu Tyr Ala Gln Glu Ser Ser Phe Leu Tyr Asp Cys Leu
675 680 685
Leu Glu Gly Phe Asn Asn Val Pro Glu Asp Ser Ile Asn Arg Gly Gly
690 695 700
Val Gly Phe Lys Lys His Asp Asn Asp Ile Val Glu Leu Thr Pro Thr
705 710 715 720
Asp Asp Leu Arg Arg Arg Leu Asp Phe Leu Pro Gln Asp Tyr Val Ala
725 730 735
Ala Arg Lys Val Lys Glu Asp Leu Leu Leu Ala Ser Thr Leu Met Arg
740 745 750
Gly Gln Glu Arg Leu Asn Ala Ala Arg Thr Gly Glu Asp Gly Ser Thr
755 760 765
Trp Pro Ser Ala His Tyr Leu Gly Pro Leu His Pro Val Thr Ser Trp
770 775 780
Ala Ala Asp Arg Ala Leu Ala Thr Met Pro Arg Ser Glu Ile Pro Ala
785 790 795 800
Ala Ser Gly Lys Val Thr Glu Pro Thr Val Leu Leu Met Ser Thr Leu
805 810 815
Ser Asn Arg Arg Gly Gln Ile Val Ser Arg Ser Phe Val Ala Ser Ser
820 825 830
Gly Pro Phe Asp Thr Glu Val Leu Ser Asp Pro Ile Gln Trp Leu His
835 840 845
Ser Ile Gly Leu Asp Glu Thr Ala Ile Asn Pro Gly Thr Ala Ala Leu
850 855 860
Pro Asp Asp Ile Glu Gln Leu Ile Ser Leu Ala Val Gln Ala Ala Arg
865 870 875 880
Gly Glu Ile Arg Pro Leu Met Ile Ala Ala Arg Ala Gln Ala Gln Thr
885 890 895
Arg Val Glu His Trp Ala Lys Arg Ala Glu Ala Trp Asn Asn Lys Arg
900 905 910
Ser Gly Ala Ala Ser Thr Ser Arg Thr Ala Arg Thr Ala Lys Leu Ile
915 920 925
Glu Glu Gln Gln Lys Leu Ser Asn Ala Leu Glu Pro Asp Arg Glu Leu
930 935 940
Ile Arg Pro Leu Ala Val Ile Leu Pro Gln Pro Ala Thr Leu Asn Thr
945 950 955 960
Glu Val
<210> 97
<211> 1624
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1594)
<223> RXA03793
<400> 97
atggaaagaa gctaggcgga aagggcgtta agtacttgcc atttaatcct cagcatcact 60
cggatcagtc ggagatgtcg atgaaaatgc accaggagcc gtg gag agc agc atg 115
Val Glu Ser Ser Met
1 5
gta gaa aac aac gta gca aaa aag acg gtc gct aaa aag acc gca cgc 163
Val Glu Asn Asn Val Ala Lys Lys Thr Val Ala Lys Lys Thr Ala Arg
10 15 20
aag acc gca cgc aaa gca gcc ccg cgc gtg gca acc cca ttg gga gtc 211
Lys Thr Ala Arg Lys Ala Ala Pro Arg Val Ala Thr Pro Leu Gly Val
25 30 35
gca tct gag tct ccc att tcg gcc acc cct gcg cgc agc atc gat gga 259
Ala Ser Glu Ser Pro Ile Ser Ala Thr Pro Ala Arg Ser Ile Asp Gly
40 45 50
acc tca acc cct gtt gaa gct gct gac acc ata gag acc acc gcc cct 307
Thr Ser Thr Pro Val Glu Ala Ala Asp Thr Ile Glu Thr Thr Ala Pro
55 60 65
gca gcg aag gct cct gcg gcc aag gct ccc gct aaa aag gtt gcc aag 355
Ala Ala Lys Ala Pro Ala Ala Lys Ala Pro Ala Lys Lys Val Ala Lys
70 75 80 85
aag aca gct cgc aag gca cct gcg aaa aag act gtc gcc aag aaa gcc 403
Lys Thr Ala Arg Lys Ala Pro Ala Lys Lys Thr Val Ala Lys Lys Ala
90 95 100
aca acc gcc aag gct gca cct gca act gcc aag gac gaa aac gca cct 451
Thr Thr Ala Lys Ala Ala Pro Ala Thr Ala Lys Asp Glu Asn Ala Pro
105 110 115
gtt gat gac gac gag gag aac ctc gct cag gat gaa cag gac ttc gac 499
Val Asp Asp Asp Glu Glu Asn Leu Ala Gln Asp Glu Gln Asp Phe Asp
120 125 130
ggc gat gac ttc gta gac ggc atc gaa gac gaa gaa gat gaa gac ggc 547
Gly Asp Asp Phe Val Asp Gly Ile Glu Asp Glu Glu Asp Glu Asp Gly
135 140 145
gtc gaa gcc ctc ggt gaa gaa agc gaa gac gac gaa gag gac ggc tca 595
Val Glu Ala Leu Gly Glu Glu Ser Glu Asp Asp Glu Glu Asp Gly Ser
150 155 160 165
tcc gtt tgg gat gaa gac gaa tcc gca acc ctg cgt cag gca cgt aaa 643
Ser Val Trp Asp Glu Asp Glu Ser Ala Thr Leu Arg Gln Ala Arg Lys
170 175 180
gat gcc gag ctc acc gct tcc gcc gac tct gtt cgc gct tac ctg aag 691
Asp Ala Glu Leu Thr Ala Ser Ala Asp Ser Val Arg Ala Tyr Leu Lys
185 190 195
caa atc ggt aaa gtt gcc ctg ctg aac gct gaa cag gaa gtc tcc ctg 739
Gln Ile Gly Lys Val Ala Leu Leu Asn Ala Glu Gln Glu Val Ser Leu
200 205 210
gca aag cgc atc gaa gca ggc ctt tac gcc acc cac cgc atg gag gaa 787
Ala Lys Arg Ile Glu Ala Gly Leu Tyr Ala Thr His Arg Met Glu Glu
215 220 225
atg gaa gaa gct ttc gca gcc ggt gac aag gac gcg aaa ctc acc cca 835
Met Glu Glu Ala Phe Ala Ala Gly Asp Lys Asp Ala Lys Leu Thr Pro
230 235 240 245
gcc gtc aag cgt gac ctc cgc gcc atc gct cgt gac ggc cgc aag gcg 883
Ala Val Lys Arg Asp Leu Arg Ala Ile Ala Arg Asp Gly Arg Lys Ala
250 255 260
aaa aac cac ctc ctg gaa gcc aac ctt cgt ctg gtt gtc tcc ctg gca 931
Lys Asn His Leu Leu Glu Ala Asn Leu Arg Leu Val Val Ser Leu Ala
265 270 275
aag cgc tac acc ggc cgt ggc atg gca ttc ctg gac ctc atc cag gaa 979
Lys Arg Tyr Thr Gly Arg Gly Met Ala Phe Leu Asp Leu Ile Gln Glu
280 285 290
ggc aac ctc ggt ctg att cgt gcc gta gag aag ttc gac tac tcc aag 1027
Gly Asn Leu Gly Leu Ile Arg Ala Val Glu Lys Phe Asp Tyr Ser Lys
295 300 305
ggc tac aag ttc tcc acc tac gca acc tgg tgg atc cgt cag gca atc 1075
Gly Tyr Lys Phe Ser Thr Tyr Ala Thr Trp Trp Ile Arg Gln Ala Ile
310 315 320 325
acc cgc gcc atg gcc gac caa gca cga acc atc cgt atc cca gtc cac 1123
Thr Arg Ala Met Ala Asp Gln Ala Arg Thr Ile Arg Ile Pro Val His
330 335 340
atg gtt gaa gtg atc aac aaa ctt ggt cgc atc caa cgt gaa ctc ctt 1171
Met Val Glu Val Ile Asn Lys Leu Gly Arg Ile Gln Arg Glu Leu Leu
345 350 355
cag gaa ctc ggc cgc gaa cca acc cca cag gaa ctg tcc aaa gaa atg 1219
Gln Glu Leu Gly Arg Glu Pro Thr Pro Gln Glu Leu Ser Lys Glu Met
360 365 370
gac atc tcc gag gaa aag gta ctg gaa atc cag cag tac gcc cgc gaa 1267
Asp Ile Ser Glu Glu Lys Val Leu Glu Ile Gln Gln Tyr Ala Arg Glu
375 380 385
cca atc tcc ctg gac caa acc atc ggc gac gaa ggc gac agc cag ctc 1315
Pro Ile Ser Leu Asp Gln Thr Ile Gly Asp Glu Gly Asp Ser Gln Leu
390 395 400 405
ggc gac ttc atc gaa gac tcc gaa gcc gtc gtc gca gtc gac gcc gtc 1363
Gly Asp Phe Ile Glu Asp Ser Glu Ala Val Val Ala Val Asp Ala Val
410 415 420
tca ttc acc ctg ctg caa gac cag cta cag gac gtc cta gag acc ctc 1411
Ser Phe Thr Leu Leu Gln Asp Gln Leu Gln Asp Val Leu Glu Thr Leu
425 430 435
tcc gaa cgt gaa gcc ggc gtg gtt aaa ctc cgc ttc gga ctc acc gac 1459
Ser Glu Arg Glu Ala Gly Val Val Lys Leu Arg Phe Gly Leu Thr Asp
440 445 450
gga atg cca cgc act tta gac gaa atc ggc caa gtt tac ggt gtc acc 1507
Gly Met Pro Arg Thr Leu Asp Glu Ile Gly Gln Val Tyr Gly Val Thr
455 460 465
cgt gag cgc atc cgc cag att gag tcc aag acc atg tct aag ctg cgc 1555
Arg Glu Arg Ile Arg Gln Ile Glu Ser Lys Thr Met Ser Lys Leu Arg
470 475 480 485
cac cca tca cgc tcc cag gtc ctt cgc gac tac ctg gac taaaacccca 1604
His Pro Ser Arg Ser Gln Val Leu Arg Asp Tyr Leu Asp
490 495
gtcgggctca agaccgggcc 1624
<210> 98
<211> 498
<212> PRT
<213> Corynebacterium glutamicum
<400> 98
Val Glu Ser Ser Met Val Glu Asn Asn Val Ala Lys Lys Thr Val Ala
1 5 10 15
Lys Lys Thr Ala Arg Lys Thr Ala Arg Lys Ala Ala Pro Arg Val Ala
20 25 30
Thr Pro Leu Gly Val Ala Ser Glu Ser Pro Ile Ser Ala Thr Pro Ala
35 40 45
Arg Ser Ile Asp Gly Thr Ser Thr Pro Val Glu Ala Ala Asp Thr Ile
50 55 60
Glu Thr Thr Ala Pro Ala Ala Lys Ala Pro Ala Ala Lys Ala Pro Ala
65 70 75 80
Lys Lys Val Ala Lys Lys Thr Ala Arg Lys Ala Pro Ala Lys Lys Thr
85 90 95
Val Ala Lys Lys Ala Thr Thr Ala Lys Ala Ala Pro Ala Thr Ala Lys
100 105 110
Asp Glu Asn Ala Pro Val Asp Asp Asp Glu Glu Asn Leu Ala Gln Asp
115 120 125
Glu Gln Asp Phe Asp Gly Asp Asp Phe Val Asp Gly Ile Glu Asp Glu
130 135 140
Glu Asp Glu Asp Gly Val Glu Ala Leu Gly Glu Glu Ser Glu Asp Asp
145 150 155 160
Glu Glu Asp Gly Ser Ser Val Trp Asp Glu Asp Glu Ser Ala Thr Leu
165 170 175
Arg Gln Ala Arg Lys Asp Ala Glu Leu Thr Ala Ser Ala Asp Ser Val
180 185 190
Arg Ala Tyr Leu Lys Gln Ile Gly Lys Val Ala Leu Leu Asn Ala Glu
195 200 205
Gln Glu Val Ser Leu Ala Lys Arg Ile Glu Ala Gly Leu Tyr Ala Thr
210 215 220
His Arg Met Glu Glu Met Glu Glu Ala Phe Ala Ala Gly Asp Lys Asp
225 230 235 240
Ala Lys Leu Thr Pro Ala Val Lys Arg Asp Leu Arg Ala Ile Ala Arg
245 250 255
Asp Gly Arg Lys Ala Lys Asn His Leu Leu Glu Ala Asn Leu Arg Leu
260 265 270
Val Val Ser Leu Ala Lys Arg Tyr Thr Gly Arg Gly Met Ala Phe Leu
275 280 285
Asp Leu Ile Gln Glu Gly Asn Leu Gly Leu Ile Arg Ala Val Glu Lys
290 295 300
Phe Asp Tyr Ser Lys Gly Tyr Lys Phe Ser Thr Tyr Ala Thr Trp Trp
305 310 315 320
Ile Arg Gln Ala Ile Thr Arg Ala Met Ala Asp Gln Ala Arg Thr Ile
325 330 335
Arg Ile Pro Val His Met Val Glu Val Ile Asn Lys Leu Gly Arg Ile
340 345 350
Gln Arg Glu Leu Leu Gln Glu Leu Gly Arg Glu Pro Thr Pro Gln Glu
355 360 365
Leu Ser Lys Glu Met Asp Ile Ser Glu Glu Lys Val Leu Glu Ile Gln
370 375 380
Gln Tyr Ala Arg Glu Pro Ile Ser Leu Asp Gln Thr Ile Gly Asp Glu
385 390 395 400
Gly Asp Ser Gln Leu Gly Asp Phe Ile Glu Asp Ser Glu Ala Val Val
405 410 415
Ala Val Asp Ala Val Ser Phe Thr Leu Leu Gln Asp Gln Leu Gln Asp
420 425 430
Val Leu Glu Thr Leu Ser Glu Arg Glu Ala Gly Val Val Lys Leu Arg
435 440 445
Phe Gly Leu Thr Asp Gly Met Pro Arg Thr Leu Asp Glu Ile Gly Gln
450 455 460
Val Tyr Gly Val Thr Arg Glu Arg Ile Arg Gln Ile Glu Ser Lys Thr
465 470 475 480
Met Ser Lys Leu Arg His Pro Ser Arg Ser Gln Val Leu Arg Asp Tyr
485 490 495
Leu Asp
<210> 99
<211> 1234
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (101)..(1204)
<223> RXA06048
<400> 99
agccgctgct gcagcgtgtc agacattttg gccgtcacct tatcaagttt gtggtgctat 60
cttagagcgc tatatccagc agggtgccca gtttgggtgg atg ttg ttg ctt act 115
Met Leu Leu Leu Thr
1 5
gaa ggc caa gcg ctt aac cct gat ggt cag gga tat cgt cag cgg ttt 163
Glu Gly Gln Ala Leu Asn Pro Asp Gly Gln Gly Tyr Arg Gln Arg Phe
10 15 20
atg aat ggg ttt atc tat tgg cat cct tct act ggt gcg cac gcg gtt 211
Met Asn Gly Phe Ile Tyr Trp His Pro Ser Thr Gly Ala His Ala Val
25 30 35
aat aat tac agt gca caa gtc tgg gag cgt aac ggt tgg gag tct ggg 259
Asn Asn Tyr Ser Ala Gln Val Trp Glu Arg Asn Gly Trp Glu Ser Gly
40 45 50
tgg atg ggg tat ccc act ggt ggt gaa gtc cct gtg tct ggg tct aat 307
Trp Met Gly Tyr Pro Thr Gly Gly Glu Val Pro Val Ser Gly Ser Asn
55 60 65
ccg att gat ggt gag ttg agt ggg tgg gtg caa acc ttc caa ggt ggg 355
Pro Ile Asp Gly Glu Leu Ser Gly Trp Val Gln Thr Phe Gln Gly Gly
70 75 80 85
cga gtg tat cgc agt ccg gta ttg gac ggt ttc cag gtg gcc agt att 403
Arg Val Tyr Arg Ser Pro Val Leu Asp Gly Phe Gln Val Ala Ser Ile
90 95 100
aat ggg ctg atc ttg gat aaa tgg ctt gaa ttg ggt ggt cct gat agt 451
Asn Gly Leu Ile Leu Asp Lys Trp Leu Glu Leu Gly Gly Pro Asp Ser
105 110 115
gac ctt ggt ttt ccc att gcg gat gag gct gtg aca gct gac ggt gtg 499
Asp Leu Gly Phe Pro Ile Ala Asp Glu Ala Val Thr Ala Asp Gly Val
120 125 130
ggc aga ttt tct gtt ttc cag aac gga gtt gtc tac tgg cat ccg caa 547
Gly Arg Phe Ser Val Phe Gln Asn Gly Val Val Tyr Trp His Pro Gln
135 140 145
cac gga gct cac cct ata tta ggg aat ata tac agc atc tgg aga gaa 595
His Gly Ala His Pro Ile Leu Gly Asn Ile Tyr Ser Ile Trp Arg Glu
150 155 160 165
gaa gga gct gag agt ggg gaa ttc ggt tac cct atc ggc gat cca gaa 643
Glu Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro Ile Gly Asp Pro Glu
170 175 180
aag tat aca gaa aac atg gct aat cag gta ttc gaa aaa ggc gaa ctt 691
Lys Tyr Thr Glu Asn Met Ala Asn Gln Val Phe Glu Lys Gly Glu Leu
185 190 195
gca gct aac cta tac ccc aat cct ctt gag gct ttt att gag ttt tta 739
Ala Ala Asn Leu Tyr Pro Asn Pro Leu Glu Ala Phe Ile Glu Phe Leu
200 205 210
ccc ttt gct aat ctt gag gaa gca ata gag tat ttt gag aac gga ttg 787
Pro Phe Ala Asn Leu Glu Glu Ala Ile Glu Tyr Phe Glu Asn Gly Leu
215 220 225
tca aat tct cgt gta gag gcg aat tca ctt aac gcc aag aaa gat tcg 835
Ser Asn Ser Arg Val Glu Ala Asn Ser Leu Asn Ala Lys Lys Asp Ser
230 235 240 245
att caa tgt caa tcg caa tcc gct aac att cat gtg aga acg aag agt 883
Ile Gln Cys Gln Ser Gln Ser Ala Asn Ile His Val Arg Thr Lys Ser
250 255 260
gac gga gtc ggg att agg gtt cca aag att ggg ttt aag gct agg atg 931
Asp Gly Val Gly Ile Arg Val Pro Lys Ile Gly Phe Lys Ala Arg Met
265 270 275
gat tgc gac ctt cct gga act gtc tca gat gta gtg ggg tat gga tgg 979
Asp Cys Asp Leu Pro Gly Thr Val Ser Asp Val Val Gly Tyr Gly Trp
280 285 290
att tac tac gac tat tgg gga cga tgg gct caa gca gca tat gca caa 1027
Ile Tyr Tyr Asp Tyr Trp Gly Arg Trp Ala Gln Ala Ala Tyr Ala Gln
295 300 305
caa ttc ttc ggt aat agg aat tct gtt gtg caa acc aat tta gag gcg 1075
Gln Phe Phe Gly Asn Arg Asn Ser Val Val Gln Thr Asn Leu Glu Ala
310 315 320 325
ggt tgc agc ggg gag aag aat aca tta ttt tgg ggt act tca tat ttt 1123
Gly Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp Gly Thr Ser Tyr Phe
330 335 340
cag gtg act tat gaa ggt cag ccg tat ttc ggt cag tca gca act aac 1171
Gln Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly Gln Ser Ala Thr Asn
345 350 355
tac gct tat ctt ccg tgt acg ata gac cgt agt taacataagg aatggaatag 1224
Tyr Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser
360 365
gagaattgcg 1234
<210> 100
<211> 368
<212> PRT
<213> Corynebacterium glutamicum
<400> 100
Met Leu Leu Leu Thr Glu Gly Gln Ala Leu Asn Pro Asp Gly Gln Gly
1 5 10 15
Tyr Arg Gln Arg Phe Met Asn Gly Phe Ile Tyr Trp His Pro Ser Thr
20 25 30
Gly Ala His Ala Val Asn Asn Tyr Ser Ala Gln Val Trp Glu Arg Asn
35 40 45
Gly Trp Glu Ser Gly Trp Met Gly Tyr Pro Thr Gly Gly Glu Val Pro
50 55 60
Val Ser Gly Ser Asn Pro Ile Asp Gly Glu Leu Ser Gly Trp Val Gln
65 70 75 80
Thr Phe Gln Gly Gly Arg Val Tyr Arg Ser Pro Val Leu Asp Gly Phe
85 90 95
Gln Val Ala Ser Ile Asn Gly Leu Ile Leu Asp Lys Trp Leu Glu Leu
100 105 110
Gly Gly Pro Asp Ser Asp Leu Gly Phe Pro Ile Ala Asp Glu Ala Val
115 120 125
Thr Ala Asp Gly Val Gly Arg Phe Ser Val Phe Gln Asn Gly Val Val
130 135 140
Tyr Trp His Pro Gln His Gly Ala His Pro Ile Leu Gly Asn Ile Tyr
145 150 155 160
Ser Ile Trp Arg Glu Glu Gly Ala Glu Ser Gly Glu Phe Gly Tyr Pro
165 170 175
Ile Gly Asp Pro Glu Lys Tyr Thr Glu Asn Met Ala Asn Gln Val Phe
180 185 190
Glu Lys Gly Glu Leu Ala Ala Asn Leu Tyr Pro Asn Pro Leu Glu Ala
195 200 205
Phe Ile Glu Phe Leu Pro Phe Ala Asn Leu Glu Glu Ala Ile Glu Tyr
210 215 220
Phe Glu Asn Gly Leu Ser Asn Ser Arg Val Glu Ala Asn Ser Leu Asn
225 230 235 240
Ala Lys Lys Asp Ser Ile Gln Cys Gln Ser Gln Ser Ala Asn Ile His
245 250 255
Val Arg Thr Lys Ser Asp Gly Val Gly Ile Arg Val Pro Lys Ile Gly
260 265 270
Phe Lys Ala Arg Met Asp Cys Asp Leu Pro Gly Thr Val Ser Asp Val
275 280 285
Val Gly Tyr Gly Trp Ile Tyr Tyr Asp Tyr Trp Gly Arg Trp Ala Gln
290 295 300
Ala Ala Tyr Ala Gln Gln Phe Phe Gly Asn Arg Asn Ser Val Val Gln
305 310 315 320
Thr Asn Leu Glu Ala Gly Cys Ser Gly Glu Lys Asn Thr Leu Phe Trp
325 330 335
Gly Thr Ser Tyr Phe Gln Val Thr Tyr Glu Gly Gln Pro Tyr Phe Gly
340 345 350
Gln Ser Ala Thr Asn Tyr Ala Tyr Leu Pro Cys Thr Ile Asp Arg Ser
355 360 365
<210> 101
<211> 4560
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (1)..(4560)
<223> RXA07005
<400> 101
atg gct aaa agc att ctt tcc cga ttc cga cct caa gta gcg gag tgg 48
Met Ala Lys Ser Ile Leu Ser Arg Phe Arg Pro Gln Val Ala Glu Trp
1 5 10 15
ttc cgg gat gtc ttt gca tct ccg acc cct gtt cag gag gga acg tgg 96
Phe Arg Asp Val Phe Ala Ser Pro Thr Pro Val Gln Glu Gly Thr Trp
20 25 30
gag gcg gta tct aag ggg aag aat gcc ctc gtg gtg gcg ccg acc ggt 144
Glu Ala Val Ser Lys Gly Lys Asn Ala Leu Val Val Ala Pro Thr Gly
35 40 45
agt ggt aaa acc ttg gct gcg ttt ttg tgg gcg tta gat tcc ctc act 192
Ser Gly Lys Thr Leu Ala Ala Phe Leu Trp Ala Leu Asp Ser Leu Thr
50 55 60
gaa caa aca ggt caa cag gtt tta gac acg gga aca ccg gtg cct gtt 240
Glu Gln Thr Gly Gln Gln Val Leu Asp Thr Gly Thr Pro Val Pro Val
65 70 75 80
cgt ggt ggg aaa gtg aaa gtg ctc tac att tcc cca ctc aaa gcg ctt 288
Arg Gly Gly Lys Val Lys Val Leu Tyr Ile Ser Pro Leu Lys Ala Leu
85 90 95
ggc gtg gat gta gaa aat aat ctg cgt gca ccg ttg acc ggt att gcg 336
Gly Val Asp Val Glu Asn Asn Leu Arg Ala Pro Leu Thr Gly Ile Ala
100 105 110
agg act gcc tct cgg atg ggt ttg gat gtg ccc aat atc act gtg gcg 384
Arg Thr Ala Ser Arg Met Gly Leu Asp Val Pro Asn Ile Thr Val Ala
115 120 125
gtt cgt tcg ggt gat acg cca tcg gcg gag cgg gcc cgg cag gtg cgt 432
Val Arg Ser Gly Asp Thr Pro Ser Ala Glu Arg Ala Arg Gln Val Arg
130 135 140
aag cct cca gac att ttg atc acc act ccg gag tcg gcg tat ttg atg 480
Lys Pro Pro Asp Ile Leu Ile Thr Thr Pro Glu Ser Ala Tyr Leu Met
145 150 155 160
ttg acc tca aaa gcg ggg gcg acc ctt tcg gat gtt gat gtg gtg atc 528
Leu Thr Ser Lys Ala Gly Ala Thr Leu Ser Asp Val Asp Val Val Ile
165 170 175
atc gat gaa atc cac gcc atg gcc gga acc aaa cgg gga gtg cat ctg 576
Ile Asp Glu Ile His Ala Met Ala Gly Thr Lys Arg Gly Val His Leu
180 185 190
gcg ttg acg ctg gag cgt ttg gaa aag ctc gtg ggg cgg cct gtg cag 624
Ala Leu Thr Leu Glu Arg Leu Glu Lys Leu Val Gly Arg Pro Val Gln
195 200 205
cga gtt ggt ttg tct gca acg gtg cgt cct ttg gaa acg gtg gcg ggt 672
Arg Val Gly Leu Ser Ala Thr Val Arg Pro Leu Glu Thr Val Ala Gly
210 215 220
ttc ttg ggc ggt ggc aga ccc gtt gag att gtg gct cca cct gcg gag 720
Phe Leu Gly Gly Gly Arg Pro Val Glu Ile Val Ala Pro Pro Ala Glu
225 230 235 240
aaa aag tgg gat ctc act gtc act gtg ccg gtg gaa gac atg tcg gat 768
Lys Lys Trp Asp Leu Thr Val Thr Val Pro Val Glu Asp Met Ser Asp
245 250 255
ttg ccg gtt cag gag ccg gga tca act att ggt gaa cta gtc atg gat 816
Leu Pro Val Gln Glu Pro Gly Ser Thr Ile Gly Glu Leu Val Met Asp
260 265 270
gat ccg ttg ggg att act ggc gaa tca gcg ctg cct act caa ggc tcg 864
Asp Pro Leu Gly Ile Thr Gly Glu Ser Ala Leu Pro Thr Gln Gly Ser
275 280 285
att tgg cca cac att gag cag cag gtg tac aac cag gtg atg tcg gcg 912
Ile Trp Pro His Ile Glu Gln Gln Val Tyr Asn Gln Val Met Ser Ala
290 295 300
aaa tcg acc atc gtg ttt gta aat tcc agg cgt tcc gcg gag cgt tta 960
Lys Ser Thr Ile Val Phe Val Asn Ser Arg Arg Ser Ala Glu Arg Leu
305 310 315 320
acc agt cgg ttg aat gaa atc tgg gcg atg gaa cac gat ccg gaa tcg 1008
Thr Ser Arg Leu Asn Glu Ile Trp Ala Met Glu His Asp Pro Glu Ser
325 330 335
ctg tcg ccg cag ctg cga aga gat ccg gcg cag att atg tcg tca gcg 1056
Leu Ser Pro Gln Leu Arg Arg Asp Pro Ala Gln Ile Met Ser Ser Ala
340 345 350
gat gtg gca gga aaa gca cca cag gtg atc gca cgt gcg cac cac gga 1104
Asp Val Ala Gly Lys Ala Pro Gln Val Ile Ala Arg Ala His His Gly
355 360 365
tcc gta tcc aaa gat gaa cgt gcc acc acc gaa acc atg ctg aag gaa 1152
Ser Val Ser Lys Asp Glu Arg Ala Thr Thr Glu Thr Met Leu Lys Glu
370 375 380
ggt cgg ttg cgc gca gtt att tcc acc tcc tcg ctg gag ttg ggc att 1200
Gly Arg Leu Arg Ala Val Ile Ser Thr Ser Ser Leu Glu Leu Gly Ile
385 390 395 400
gat atg ggt gcc gtg gac ctg gtg att cag gtg gaa tcg cca ccg tcc 1248
Asp Met Gly Ala Val Asp Leu Val Ile Gln Val Glu Ser Pro Pro Ser
405 410 415
gtg gca agt ggc ctg cag cgc gtg ggg cgt gcg ggg cac acg gtg ggg 1296
Val Ala Ser Gly Leu Gln Arg Val Gly Arg Ala Gly His Thr Val Gly
420 425 430
gcg acg tcg ata ggc tcc ttt tat ccc aag cac cgc tcc gac ttg gtg 1344
Ala Thr Ser Ile Gly Ser Phe Tyr Pro Lys His Arg Ser Asp Leu Val
435 440 445
caa acc gcg gtg acc gtg cag cgg atg aag gaa ggg ctg atc gaa gag 1392
Gln Thr Ala Val Thr Val Gln Arg Met Lys Glu Gly Leu Ile Glu Glu
450 455 460
atc cac gtg ccc aaa aac gcg ctt gat gta ctg gca cag cag acg gtg 1440
Ile His Val Pro Lys Asn Ala Leu Asp Val Leu Ala Gln Gln Thr Val
465 470 475 480
gcg gct gtc tcg att aaa gat gtg cag gtc gat gag tgg tac gag act 1488
Ala Ala Val Ser Ile Lys Asp Val Gln Val Asp Glu Trp Tyr Glu Thr
485 490 495
att cgc aag gcg tat ccg tac cgg gat ttg gcg cgc gaa gtc ttc gat 1536
Ile Arg Lys Ala Tyr Pro Tyr Arg Asp Leu Ala Arg Glu Val Phe Asp
500 505 510
tcc gtc atc gac ctg gtc agc ggt gtg tat ccc tcc aca gat ttt gcc 1584
Ser Val Ile Asp Leu Val Ser Gly Val Tyr Pro Ser Thr Asp Phe Ala
515 520 525
gag ctg aag cca cgt gtg gtg tac gac cgg gtt tca ggc gtg ctg gag 1632
Glu Leu Lys Pro Arg Val Val Tyr Asp Arg Val Ser Gly Val Leu Glu
530 535 540
ggc cgg cca gga tcc caa cgc gta gca gtg acc agt ggc gga aca att 1680
Gly Arg Pro Gly Ser Gln Arg Val Ala Val Thr Ser Gly Gly Thr Ile
545 550 555 560
ccc gat cga gga atg ttc gga gtc ttc ctc gtc ggc gat ggt ccc cgg 1728
Pro Asp Arg Gly Met Phe Gly Val Phe Leu Val Gly Asp Gly Pro Arg
565 570 575
cgc gtc ggc gag ctc gat gag gaa atg gtc tac gaa tcc cgc gtg ggc 1776
Arg Val Gly Glu Leu Asp Glu Glu Met Val Tyr Glu Ser Arg Val Gly
580 585 590
gat gtg ttt acg ctc ggg gcg tcg agt tgg cgg att gaa gag atc acc 1824
Asp Val Phe Thr Leu Gly Ala Ser Ser Trp Arg Ile Glu Glu Ile Thr
595 600 605
cgc gac cag gta ctg gtc act ccc gcg ccg ggt cac acg ggt cgg ctg 1872
Arg Asp Gln Val Leu Val Thr Pro Ala Pro Gly His Thr Gly Arg Leu
610 615 620
cct ttt tgg acg ggc gat gcc gca ggc cgg ccc gct gag ctg ggt aaa 1920
Pro Phe Trp Thr Gly Asp Ala Ala Gly Arg Pro Ala Glu Leu Gly Lys
625 630 635 640
gct tta ggc gct ttt cga cgc tcg acc ctc acc gat cca tcc agc tcc 1968
Ala Leu Gly Ala Phe Arg Arg Ser Thr Leu Thr Asp Pro Ser Ser Ser
645 650 655
ggc ttg gaa ggc tgg gcg cac gac aac ctg atc gcc ttt tta cag gag 2016
Gly Leu Glu Gly Trp Ala His Asp Asn Leu Ile Ala Phe Leu Gln Glu
660 665 670
cag gaa gaa tcc acc ggt gtg ttg ccg gat gag aag acg ttg gtg ttg 2064
Gln Glu Glu Ser Thr Gly Val Leu Pro Asp Glu Lys Thr Leu Val Leu
675 680 685
gag cgt ttc aaa gat gaa cta ggc gac tgg cgc att gtc ctg cac act 2112
Glu Arg Phe Lys Asp Glu Leu Gly Asp Trp Arg Ile Val Leu His Thr
690 695 700
cct tat gga cga gga gta aac gca gca tgg gct ttg gcc gtc ggg gcg 2160
Pro Tyr Gly Arg Gly Val Asn Ala Ala Trp Ala Leu Ala Val Gly Ala
705 710 715 720
aaa atc gct gaa gag acc ggc atg gat gcg caa gcc gtg gca ggt gat 2208
Lys Ile Ala Glu Glu Thr Gly Met Asp Ala Gln Ala Val Ala Gly Asp
725 730 735
gat ggc att gtg ctt cgg ttg ccg gaa ggg gat gaa gat ccc agc gca 2256
Asp Gly Ile Val Leu Arg Leu Pro Glu Gly Asp Glu Asp Pro Ser Ala
740 745 750
gcg ttg ttt atg ttt gag gcg gaa gag atc gaa acg cta gtg aca gag 2304
Ala Leu Phe Met Phe Glu Ala Glu Glu Ile Glu Thr Leu Val Thr Glu
755 760 765
cag gtg ggt aac tct gcg ctg ttt gcc agc agg ttc cgt gaa tgc gcc 2352
Gln Val Gly Asn Ser Ala Leu Phe Ala Ser Arg Phe Arg Glu Cys Ala
770 775 780
gcg agg gcc cta ttg ctg ccg aga cga aac ccc ggc aag cgc gca ccg 2400
Ala Arg Ala Leu Leu Leu Pro Arg Arg Asn Pro Gly Lys Arg Ala Pro
785 790 795 800
ctg tgg cag caa cga caa cga gca gca cag ctt ctt gat gtg gcc aga 2448
Leu Trp Gln Gln Arg Gln Arg Ala Ala Gln Leu Leu Asp Val Ala Arg
805 810 815
aag tac ccg agt ttc ccg atc att ttg gaa aca gtg cgc gaa tgt ctt 2496
Lys Tyr Pro Ser Phe Pro Ile Ile Leu Glu Thr Val Arg Glu Cys Leu
820 825 830
caa gat gtt tac gat ctg ccc gct ctg aag aat ctc att gag gat cta 2544
Gln Asp Val Tyr Asp Leu Pro Ala Leu Lys Asn Leu Ile Glu Asp Leu
835 840 845
cag ctg cgg aag gta aga atc gcg gaa gtc acc acc cag cag ccc agt 2592
Gln Leu Arg Lys Val Arg Ile Ala Glu Val Thr Thr Gln Gln Pro Ser
850 855 860
cct ttt gcc tcc gca ttg ctg ttc aat tac acc ggt gca ttc atg tac 2640
Pro Phe Ala Ser Ala Leu Leu Phe Asn Tyr Thr Gly Ala Phe Met Tyr
865 870 875 880
gaa ggc gac agc ccg ctc gca gag aaa cgt gcc gca gcg ttg gcc ctg 2688
Glu Gly Asp Ser Pro Leu Ala Glu Lys Arg Ala Ala Ala Leu Ala Leu
885 890 895
gat ccg gca ctg ttg gcg aaa ttg ctg ggt gag gtg gag ctt cga caa 2736
Asp Pro Ala Leu Leu Ala Lys Leu Leu Gly Glu Val Glu Leu Arg Gln
900 905 910
tta ctg gat ccc gac atc atc gca gaa gtg cac caa caa ttg cgc agg 2784
Leu Leu Asp Pro Asp Ile Ile Ala Glu Val His Gln Gln Leu Arg Arg
915 920 925
caa ggc gat cgt gcg gcg aga aac aat gaa gaa ctc gca gat tct ttg 2832
Gln Gly Asp Arg Ala Ala Arg Asn Asn Glu Glu Leu Ala Asp Ser Leu
930 935 940
agg att tta gga ccg att cct ttg gat gaa ttg ggc gaa cac atc acc 2880
Arg Ile Leu Gly Pro Ile Pro Leu Asp Glu Leu Gly Glu His Ile Thr
945 950 955 960
ttt gaa aac cca gac ctg gag gat cga gca atg act gtt cgg atc aac 2928
Phe Glu Asn Pro Asp Leu Glu Asp Arg Ala Met Thr Val Arg Ile Asn
965 970 975
ggt cgg gaa cat tta gcg cag gtc ttg gat gca cct ttg ctt cga gat 2976
Gly Arg Glu His Leu Ala Gln Val Leu Asp Ala Pro Leu Leu Arg Asp
980 985 990
gcc tta ggt gtt ccc gta ccg cct ggt gtg cct gcg cag gta gaa acc 3024
Ala Leu Gly Val Pro Val Pro Pro Gly Val Pro Ala Gln Val Glu Thr
995 1000 1005
att acg gat gcg ttg gaa cag tta gtc aac agg tgg gtt cgt acc aga 3072
Ile Thr Asp Ala Leu Glu Gln Leu Val Asn Arg Trp Val Arg Thr Arg
1010 1015 1020
ggg cca ttt act gcg aat gat ttg gca gaa gcc ttt gga ctg ggc atc 3120
Gly Pro Phe Thr Ala Asn Asp Leu Ala Glu Ala Phe Gly Leu Gly Ile
1025 1030 1035 1040
gcc acg gcg atc acc gcc ctt caa agc gca cct gtg att gaa ggc cgc 3168
Ala Thr Ala Ile Thr Ala Leu Gln Ser Ala Pro Val Ile Glu Gly Arg
1045 1050 1055
tac cga caa ggc gtg gac gtg cag gaa tac tgt gcg aca gaa gtg ttg 3216
Tyr Arg Gln Gly Val Asp Val Gln Glu Tyr Cys Ala Thr Glu Val Leu
1060 1065 1070
tcg atc ata agg cga cgc agc ctc gca gca gcg agg aaa caa acc agg 3264
Ser Ile Ile Arg Arg Arg Ser Leu Ala Ala Ala Arg Lys Gln Thr Arg
1075 1080 1085
ccg gta tcg caa tca gcc ttt gcg cga ttc ctg ctt gat tgg caa cag 3312
Pro Val Ser Gln Ser Ala Phe Ala Arg Phe Leu Leu Asp Trp Gln Gln
1090 1095 1100
atc gca ccg gtg ggc gcc aca cct gaa ctc cga ggc gtt gat ggc acc 3360
Ile Ala Pro Val Gly Ala Thr Pro Glu Leu Arg Gly Val Asp Gly Thr
1105 1110 1115 1120
tac aca gtc att gaa caa ctc gcc ggt gta cgt ctt ccc gcc agt gcg 3408
Tyr Thr Val Ile Glu Gln Leu Ala Gly Val Arg Leu Pro Ala Ser Ala
1125 1130 1135
tgg gaa gat ctc gtg ttg ccg cgc cgg gtt gcc gac tat tca ccg atc 3456
Trp Glu Asp Leu Val Leu Pro Arg Arg Val Ala Asp Tyr Ser Pro Ile
1140 1145 1150
cat ctc gat gag ctg acc tcc aat ggg gaa gtc ctc atc gtg gga gcg 3504
His Leu Asp Glu Leu Thr Ser Asn Gly Glu Val Leu Ile Val Gly Ala
1155 1160 1165
ggc caa gcc gga agc cgc gat ccg tgg att agc ttg ctg ccc gtg gat 3552
Gly Gln Ala Gly Ser Arg Asp Pro Trp Ile Ser Leu Leu Pro Val Asp
1170 1175 1180
tat gcg gcg cag ttg gtg ggg gag gcg tcg aca agc atg agc cca ttg 3600
Tyr Ala Ala Gln Leu Val Gly Glu Ala Ser Thr Ser Met Ser Pro Leu
1185 1190 1195 1200
cag gac gcc gtg ctt gac cag ctg cgt gcg gga ggc gcc ttc ctg ttt 3648
Gln Asp Ala Val Leu Asp Gln Leu Arg Ala Gly Gly Ala Phe Leu Phe
1205 1210 1215
tct gac att ctc gaa gag aat ttc ggc tac acc aca gcc cag ctg caa 3696
Ser Asp Ile Leu Glu Glu Asn Phe Gly Tyr Thr Thr Ala Gln Leu Gln
1220 1225 1230
gaa gcg atg tgg ggg ctg gtg gaa gca ggc ctg gtc agc cct gat agc 3744
Glu Ala Met Trp Gly Leu Val Glu Ala Gly Leu Val Ser Pro Asp Ser
1235 1240 1245
ttc gcg ccg atc cgc gcg cgc cta gcg tcg gga acc acg gcg cat cgg 3792
Phe Ala Pro Ile Arg Ala Arg Leu Ala Ser Gly Thr Thr Ala His Arg
1250 1255 1260
gcg aaa cgt cga cca gcg aga tcc cgg ctg cgc acc cgc acc agc ttc 3840
Ala Lys Arg Arg Pro Ala Arg Ser Arg Leu Arg Thr Arg Thr Ser Phe
1265 1270 1275 1280
gcg agc gac gtg ccc cca gac atg cgc gga cga tgg acg ctg tcc gtg 3888
Ala Ser Asp Val Pro Pro Asp Met Arg Gly Arg Trp Thr Leu Ser Val
1285 1290 1295
caa ccc gcc gac gcc acc agc cgc tcc gtc gca cac ggc gaa ggc tgg 3936
Gln Pro Ala Asp Ala Thr Ser Arg Ser Val Ala His Gly Glu Gly Trp
1300 1305 1310
ctc gac cgc tac ggc gtg ctc acc cgc ggg agc gtc gtc gcc gaa gac 3984
Leu Asp Arg Tyr Gly Val Leu Thr Arg Gly Ser Val Val Ala Glu Asp
1315 1320 1325
atc gtc gga ggc ttc gcc ctg gcc tac aaa gtg ctc tcc ggc ttc gaa 4032
Ile Val Gly Gly Phe Ala Leu Ala Tyr Lys Val Leu Ser Gly Phe Glu
1330 1335 1340
gaa agc ggc aaa gcg atg cgc ggc tac ttc atc gaa ggg ctc ggc gcc 4080
Glu Ser Gly Lys Ala Met Arg Gly Tyr Phe Ile Glu Gly Leu Gly Ala
1345 1350 1355 1360
gcg caa ttc tcc acg ccc gcc atc atc gac cgc ctc cgc ggc cac gac 4128
Ala Gln Phe Ser Thr Pro Ala Ile Ile Asp Arg Leu Arg Gly His Asp
1365 1370 1375
gat tcc ccc gac gtc gaa ggc tgg ccc tcc ggc gcc acc gac cca gac 4176
Asp Ser Pro Asp Val Glu Gly Trp Pro Ser Gly Ala Thr Asp Pro Asp
1380 1385 1390
gtc tac ctc ata gcc gcc gcc gac ccc gca aac ccc tac ggc gcc gca 4224
Val Tyr Leu Ile Ala Ala Ala Asp Pro Ala Asn Pro Tyr Gly Ala Ala
1395 1400 1405
ctt ccc tgg cct gag cag ggg ccc agc cgc gcc gcc gga gct atg gtc 4272
Leu Pro Trp Pro Glu Gln Gly Pro Ser Arg Ala Ala Gly Ala Met Val
1410 1415 1420
gtg ctt tgc gac gga ctc ctc ctc gcc cac ctc acc cgc ggc ggg cgc 4320
Val Leu Cys Asp Gly Leu Leu Leu Ala His Leu Thr Arg Gly Gly Arg
1425 1430 1435 1440
acc ctc acc gtg ttc tcc gac aat atc ccc aaa atc gcg aca gcc cta 4368
Thr Leu Thr Val Phe Ser Asp Asn Ile Pro Lys Ile Ala Thr Ala Leu
1445 1450 1455
atc aca tac gaa agg ctc acg gta gaa aaa atc aac ggc gac aac gtc 4416
Ile Thr Tyr Glu Arg Leu Thr Val Glu Lys Ile Asn Gly Asp Asn Val
1460 1465 1470
ttc gac tcc cca ctc ctg gaa caa ttc cgc aaa cac ggc gcc acc atc 4464
Phe Asp Ser Pro Leu Leu Glu Gln Phe Arg Lys His Gly Ala Thr Ile
1475 1480 1485
acc ccg aag gga atg cga ttt cga cca cca gtg gca cgg gaa acc ccc 4512
Thr Pro Lys Gly Met Arg Phe Arg Pro Pro Val Ala Arg Glu Thr Pro
1490 1495 1500
tca gat acg ctt ccc acc agg act ttt cgt gga ggc ttc gga cgg cgc 4560
Ser Asp Thr Leu Pro Thr Arg Thr Phe Arg Gly Gly Phe Gly Arg Arg
1505 1510 1515 1520
<210> 102
<211> 1520
<212> PRT
<213> Corynebacterium glutamicum
<400> 102
Met Ala Lys Ser Ile Leu Ser Arg Phe Arg Pro Gln Val Ala Glu Trp
1 5 10 15
Phe Arg Asp Val Phe Ala Ser Pro Thr Pro Val Gln Glu Gly Thr Trp
20 25 30
Glu Ala Val Ser Lys Gly Lys Asn Ala Leu Val Val Ala Pro Thr Gly
35 40 45
Ser Gly Lys Thr Leu Ala Ala Phe Leu Trp Ala Leu Asp Ser Leu Thr
50 55 60
Glu Gln Thr Gly Gln Gln Val Leu Asp Thr Gly Thr Pro Val Pro Val
65 70 75 80
Arg Gly Gly Lys Val Lys Val Leu Tyr Ile Ser Pro Leu Lys Ala Leu
85 90 95
Gly Val Asp Val Glu Asn Asn Leu Arg Ala Pro Leu Thr Gly Ile Ala
100 105 110
Arg Thr Ala Ser Arg Met Gly Leu Asp Val Pro Asn Ile Thr Val Ala
115 120 125
Val Arg Ser Gly Asp Thr Pro Ser Ala Glu Arg Ala Arg Gln Val Arg
130 135 140
Lys Pro Pro Asp Ile Leu Ile Thr Thr Pro Glu Ser Ala Tyr Leu Met
145 150 155 160
Leu Thr Ser Lys Ala Gly Ala Thr Leu Ser Asp Val Asp Val Val Ile
165 170 175
Ile Asp Glu Ile His Ala Met Ala Gly Thr Lys Arg Gly Val His Leu
180 185 190
Ala Leu Thr Leu Glu Arg Leu Glu Lys Leu Val Gly Arg Pro Val Gln
195 200 205
Arg Val Gly Leu Ser Ala Thr Val Arg Pro Leu Glu Thr Val Ala Gly
210 215 220
Phe Leu Gly Gly Gly Arg Pro Val Glu Ile Val Ala Pro Pro Ala Glu
225 230 235 240
Lys Lys Trp Asp Leu Thr Val Thr Val Pro Val Glu Asp Met Ser Asp
245 250 255
Leu Pro Val Gln Glu Pro Gly Ser Thr Ile Gly Glu Leu Val Met Asp
260 265 270
Asp Pro Leu Gly Ile Thr Gly Glu Ser Ala Leu Pro Thr Gln Gly Ser
275 280 285
Ile Trp Pro His Ile Glu Gln Gln Val Tyr Asn Gln Val Met Ser Ala
290 295 300
Lys Ser Thr Ile Val Phe Val Asn Ser Arg Arg Ser Ala Glu Arg Leu
305 310 315 320
Thr Ser Arg Leu Asn Glu Ile Trp Ala Met Glu His Asp Pro Glu Ser
325 330 335
Leu Ser Pro Gln Leu Arg Arg Asp Pro Ala Gln Ile Met Ser Ser Ala
340 345 350
Asp Val Ala Gly Lys Ala Pro Gln Val Ile Ala Arg Ala His His Gly
355 360 365
Ser Val Ser Lys Asp Glu Arg Ala Thr Thr Glu Thr Met Leu Lys Glu
370 375 380
Gly Arg Leu Arg Ala Val Ile Ser Thr Ser Ser Leu Glu Leu Gly Ile
385 390 395 400
Asp Met Gly Ala Val Asp Leu Val Ile Gln Val Glu Ser Pro Pro Ser
405 410 415
Val Ala Ser Gly Leu Gln Arg Val Gly Arg Ala Gly His Thr Val Gly
420 425 430
Ala Thr Ser Ile Gly Ser Phe Tyr Pro Lys His Arg Ser Asp Leu Val
435 440 445
Gln Thr Ala Val Thr Val Gln Arg Met Lys Glu Gly Leu Ile Glu Glu
450 455 460
Ile His Val Pro Lys Asn Ala Leu Asp Val Leu Ala Gln Gln Thr Val
465 470 475 480
Ala Ala Val Ser Ile Lys Asp Val Gln Val Asp Glu Trp Tyr Glu Thr
485 490 495
Ile Arg Lys Ala Tyr Pro Tyr Arg Asp Leu Ala Arg Glu Val Phe Asp
500 505 510
Ser Val Ile Asp Leu Val Ser Gly Val Tyr Pro Ser Thr Asp Phe Ala
515 520 525
Glu Leu Lys Pro Arg Val Val Tyr Asp Arg Val Ser Gly Val Leu Glu
530 535 540
Gly Arg Pro Gly Ser Gln Arg Val Ala Val Thr Ser Gly Gly Thr Ile
545 550 555 560
Pro Asp Arg Gly Met Phe Gly Val Phe Leu Val Gly Asp Gly Pro Arg
565 570 575
Arg Val Gly Glu Leu Asp Glu Glu Met Val Tyr Glu Ser Arg Val Gly
580 585 590
Asp Val Phe Thr Leu Gly Ala Ser Ser Trp Arg Ile Glu Glu Ile Thr
595 600 605
Arg Asp Gln Val Leu Val Thr Pro Ala Pro Gly His Thr Gly Arg Leu
610 615 620
Pro Phe Trp Thr Gly Asp Ala Ala Gly Arg Pro Ala Glu Leu Gly Lys
625 630 635 640
Ala Leu Gly Ala Phe Arg Arg Ser Thr Leu Thr Asp Pro Ser Ser Ser
645 650 655
Gly Leu Glu Gly Trp Ala His Asp Asn Leu Ile Ala Phe Leu Gln Glu
660 665 670
Gln Glu Glu Ser Thr Gly Val Leu Pro Asp Glu Lys Thr Leu Val Leu
675 680 685
Glu Arg Phe Lys Asp Glu Leu Gly Asp Trp Arg Ile Val Leu His Thr
690 695 700
Pro Tyr Gly Arg Gly Val Asn Ala Ala Trp Ala Leu Ala Val Gly Ala
705 710 715 720
Lys Ile Ala Glu Glu Thr Gly Met Asp Ala Gln Ala Val Ala Gly Asp
725 730 735
Asp Gly Ile Val Leu Arg Leu Pro Glu Gly Asp Glu Asp Pro Ser Ala
740 745 750
Ala Leu Phe Met Phe Glu Ala Glu Glu Ile Glu Thr Leu Val Thr Glu
755 760 765
Gln Val Gly Asn Ser Ala Leu Phe Ala Ser Arg Phe Arg Glu Cys Ala
770 775 780
Ala Arg Ala Leu Leu Leu Pro Arg Arg Asn Pro Gly Lys Arg Ala Pro
785 790 795 800
Leu Trp Gln Gln Arg Gln Arg Ala Ala Gln Leu Leu Asp Val Ala Arg
805 810 815
Lys Tyr Pro Ser Phe Pro Ile Ile Leu Glu Thr Val Arg Glu Cys Leu
820 825 830
Gln Asp Val Tyr Asp Leu Pro Ala Leu Lys Asn Leu Ile Glu Asp Leu
835 840 845
Gln Leu Arg Lys Val Arg Ile Ala Glu Val Thr Thr Gln Gln Pro Ser
850 855 860
Pro Phe Ala Ser Ala Leu Leu Phe Asn Tyr Thr Gly Ala Phe Met Tyr
865 870 875 880
Glu Gly Asp Ser Pro Leu Ala Glu Lys Arg Ala Ala Ala Leu Ala Leu
885 890 895
Asp Pro Ala Leu Leu Ala Lys Leu Leu Gly Glu Val Glu Leu Arg Gln
900 905 910
Leu Leu Asp Pro Asp Ile Ile Ala Glu Val His Gln Gln Leu Arg Arg
915 920 925
Gln Gly Asp Arg Ala Ala Arg Asn Asn Glu Glu Leu Ala Asp Ser Leu
930 935 940
Arg Ile Leu Gly Pro Ile Pro Leu Asp Glu Leu Gly Glu His Ile Thr
945 950 955 960
Phe Glu Asn Pro Asp Leu Glu Asp Arg Ala Met Thr Val Arg Ile Asn
965 970 975
Gly Arg Glu His Leu Ala Gln Val Leu Asp Ala Pro Leu Leu Arg Asp
980 985 990
Ala Leu Gly Val Pro Val Pro Pro Gly Val Pro Ala Gln Val Glu Thr
995 1000 1005
Ile Thr Asp Ala Leu Glu Gln Leu Val Asn Arg Trp Val Arg Thr Arg
1010 1015 1020
Gly Pro Phe Thr Ala Asn Asp Leu Ala Glu Ala Phe Gly Leu Gly Ile
1025 1030 1035 1040
Ala Thr Ala Ile Thr Ala Leu Gln Ser Ala Pro Val Ile Glu Gly Arg
1045 1050 1055
Tyr Arg Gln Gly Val Asp Val Gln Glu Tyr Cys Ala Thr Glu Val Leu
1060 1065 1070
Ser Ile Ile Arg Arg Arg Ser Leu Ala Ala Ala Arg Lys Gln Thr Arg
1075 1080 1085
Pro Val Ser Gln Ser Ala Phe Ala Arg Phe Leu Leu Asp Trp Gln Gln
1090 1095 1100
Ile Ala Pro Val Gly Ala Thr Pro Glu Leu Arg Gly Val Asp Gly Thr
1105 1110 1115 1120
Tyr Thr Val Ile Glu Gln Leu Ala Gly Val Arg Leu Pro Ala Ser Ala
1125 1130 1135
Trp Glu Asp Leu Val Leu Pro Arg Arg Val Ala Asp Tyr Ser Pro Ile
1140 1145 1150
His Leu Asp Glu Leu Thr Ser Asn Gly Glu Val Leu Ile Val Gly Ala
1155 1160 1165
Gly Gln Ala Gly Ser Arg Asp Pro Trp Ile Ser Leu Leu Pro Val Asp
1170 1175 1180
Tyr Ala Ala Gln Leu Val Gly Glu Ala Ser Thr Ser Met Ser Pro Leu
1185 1190 1195 1200
Gln Asp Ala Val Leu Asp Gln Leu Arg Ala Gly Gly Ala Phe Leu Phe
1205 1210 1215
Ser Asp Ile Leu Glu Glu Asn Phe Gly Tyr Thr Thr Ala Gln Leu Gln
1220 1225 1230
Glu Ala Met Trp Gly Leu Val Glu Ala Gly Leu Val Ser Pro Asp Ser
1235 1240 1245
Phe Ala Pro Ile Arg Ala Arg Leu Ala Ser Gly Thr Thr Ala His Arg
1250 1255 1260
Ala Lys Arg Arg Pro Ala Arg Ser Arg Leu Arg Thr Arg Thr Ser Phe
1265 1270 1275 1280
Ala Ser Asp Val Pro Pro Asp Met Arg Gly Arg Trp Thr Leu Ser Val
1285 1290 1295
Gln Pro Ala Asp Ala Thr Ser Arg Ser Val Ala His Gly Glu Gly Trp
1300 1305 1310
Leu Asp Arg Tyr Gly Val Leu Thr Arg Gly Ser Val Val Ala Glu Asp
1315 1320 1325
Ile Val Gly Gly Phe Ala Leu Ala Tyr Lys Val Leu Ser Gly Phe Glu
1330 1335 1340
Glu Ser Gly Lys Ala Met Arg Gly Tyr Phe Ile Glu Gly Leu Gly Ala
1345 1350 1355 1360
Ala Gln Phe Ser Thr Pro Ala Ile Ile Asp Arg Leu Arg Gly His Asp
1365 1370 1375
Asp Ser Pro Asp Val Glu Gly Trp Pro Ser Gly Ala Thr Asp Pro Asp
1380 1385 1390
Val Tyr Leu Ile Ala Ala Ala Asp Pro Ala Asn Pro Tyr Gly Ala Ala
1395 1400 1405
Leu Pro Trp Pro Glu Gln Gly Pro Ser Arg Ala Ala Gly Ala Met Val
1410 1415 1420
Val Leu Cys Asp Gly Leu Leu Leu Ala His Leu Thr Arg Gly Gly Arg
1425 1430 1435 1440
Thr Leu Thr Val Phe Ser Asp Asn Ile Pro Lys Ile Ala Thr Ala Leu
1445 1450 1455
Ile Thr Tyr Glu Arg Leu Thr Val Glu Lys Ile Asn Gly Asp Asn Val
1460 1465 1470
Phe Asp Ser Pro Leu Leu Glu Gln Phe Arg Lys His Gly Ala Thr Ile
1475 1480 1485
Thr Pro Lys Gly Met Arg Phe Arg Pro Pro Val Ala Arg Glu Thr Pro
1490 1495 1500
Ser Asp Thr Leu Pro Thr Arg Thr Phe Arg Gly Gly Phe Gly Arg Arg
1505 1510 1515 1520
<210> 103
<211> 1251
<212> DNA
<213> Corynebacterium glutamicum
<220>
<221> CDS
<222> (1)..(1251)
<223> RXA07006
<400> 103
gtg tcg tct gag aaa gct tca tca aaa tca acc cct gag gca ccg tgg 48
Val Ser Ser Glu Lys Ala Ser Ser Lys Ser Thr Pro Glu Ala Pro Trp
1 5 10 15
cca gtc cgg gaa gta aat act caa gtc aag cag tgg att gaa cgg ctt 96
Pro Val Arg Glu Val Asn Thr Gln Val Lys Gln Trp Ile Glu Arg Leu
20 25 30
ggc cat ttg tgg gtg gag ggc cag ctc gct cag att aat gtg aag ccc 144
Gly His Leu Trp Val Glu Gly Gln Leu Ala Gln Ile Asn Val Lys Pro
35 40 45
aat tgg aag ctg tcg tat ttg acg ctt cgt gat gtg gag caa gaa atg 192
Asn Trp Lys Leu Ser Tyr Leu Thr Leu Arg Asp Val Glu Gln Glu Met
50 55 60
tct gtg cag ctg acc tgc ccg acg gac att atc cgc aat cgc ccc aca 240
Ser Val Gln Leu Thr Cys Pro Thr Asp Ile Ile Arg Asn Arg Pro Thr
65 70 75 80
ccg ctc aag gat ggc gac cgc gtg att gtg tac ggc aag ccc gcg ttt 288
Pro Leu Lys Asp Gly Asp Arg Val Ile Val Tyr Gly Lys Pro Ala Phe
85 90 95
tat gca ggc cgc ggc act ttt tcg ctg tgg gtg act gat atc cgt ccc 336
Tyr Ala Gly Arg Gly Thr Phe Ser Leu Trp Val Thr Asp Ile Arg Pro
100 105 110
gtg ggt att ggt gag ttg ctg gcg cgc att gag gag ctg cgt aaa agg 384
Val Gly Ile Gly Glu Leu Leu Ala Arg Ile Glu Glu Leu Arg Lys Arg
115 120 125
ctt gcc gcg gag ggt ctt ttt gat cca gct cgg aag aag cga ctg cca 432
Leu Ala Ala Glu Gly Leu Phe Asp Pro Ala Arg Lys Lys Arg Leu Pro
130 135 140
ttt ctg ccc aac cgc gtt ggt ttg atc acg gga cgt ggt tca gcg gct 480
Phe Leu Pro Asn Arg Val Gly Leu Ile Thr Gly Arg Gly Ser Ala Ala
145 150 155 160
gag cgc gat gtg ctg agc gtg gct aag gat cgc tgg ccg gaa gtg cag 528
Glu Arg Asp Val Leu Ser Val Ala Lys Asp Arg Trp Pro Glu Val Gln
165 170 175
ttt gag gtg atc aac acg gca gtt cag ggc gct tca gct gtt cct gaa 576
Phe Glu Val Ile Asn Thr Ala Val Gln Gly Ala Ser Ala Val Pro Glu
180 185 190
atc atc gaa gcg ttg cgg gtt tta gat cag gac cct cgc gtg gat gtc 624
Ile Ile Glu Ala Leu Arg Val Leu Asp Gln Asp Pro Arg Val Asp Val
195 200 205
atc atc att gcc cgc ggc ggc ggt tct gtg gag gat ctg ctc ccc ttc 672
Ile Ile Ile Ala Arg Gly Gly Gly Ser Val Glu Asp Leu Leu Pro Phe
210 215 220
tct gag gag gcc ttg cag cgc gca gtc gcg gca gcg cag acg ccc gtg 720
Ser Glu Glu Ala Leu Gln Arg Ala Val Ala Ala Ala Gln Thr Pro Val
225 230 235 240
gtg tcc gcg att ggc cac gaa cca gat acg ccg gtg ttg gac aat gtc 768
Val Ser Ala Ile Gly His Glu Pro Asp Thr Pro Val Leu Asp Asn Val
245 250 255
gcc gac ctt cgc gcg gcg acc ccg acc gat gca gca aag cgc gtg gtg 816
Ala Asp Leu Arg Ala Ala Thr Pro Thr Asp Ala Ala Lys Arg Val Val
260 265 270
cct gat gtg gca gaa gaa cgc atg ttg atc aat cag ctt cgc agt cgt 864
Pro Asp Val Ala Glu Glu Arg Met Leu Ile Asn Gln Leu Arg Ser Arg
275 280 285
agt gcc gcg gcg ttg cgc ggt tgg gtg cag cgc gag cag cag gcg ttg 912
Ser Ala Ala Ala Leu Arg Gly Trp Val Gln Arg Glu Gln Gln Ala Leu
290 295 300
gca gcg att cgc acc agg ccg gtg ctg gct gat ccg atg acc ccg att 960
Ala Ala Ile Arg Thr Arg Pro Val Leu Ala Asp Pro Met Thr Pro Ile
305 310 315 320
aac cgc cga cgt gat gag att gcc cag gct gtg ggc ttg att agg cgc 1008
Asn Arg Arg Arg Asp Glu Ile Ala Gln Ala Val Gly Leu Ile Arg Arg
325 330 335
gat gtc acc cat ctc gtc cgc acc gag caa gca ctg gtg gcg tcg ttg 1056
Asp Val Thr His Leu Val Arg Thr Glu Gln Ala Leu Val Ala Ser Leu
340 345 350
cgc gca cag gtt tcc gcg ctc ggc ccg tcc gca acc ttg gcg cgc ggt 1104
Arg Ala Gln Val Ser Ala Leu Gly Pro Ser Ala Thr Leu Ala Arg Gly
355 360 365
tat tcc gtg gtg cag gtt att cct cgc gac ggc agc gcc ccg gaa gtg 1152
Tyr Ser Val Val Gln Val Ile Pro Arg Asp Gly Ser Ala Pro Glu Val
370 375 380
gtc acc acc atc gag caa tca ccg ccc ggc agc cag ctg cgc atc cgc 1200
Val Thr Thr Ile Glu Gln Ser Pro Pro Gly Ser Gln Leu Arg Ile Arg
385 390 395 400
gtt gcc gac ggc tcc atc act gcg gca tcc atg ggc acc cag caa gca 1248
Val Ala Asp Gly Ser Ile Thr Ala Ala Ser Met Gly Thr Gln Gln Ala
405 410 415
aac 1251
Asn
<210> 104
<211> 417
<212> PRT
<213> Corynebacterium glutamicum
<400> 104
Val Ser Ser Glu Lys Ala Ser Ser Lys Ser Thr Pro Glu Ala Pro Trp
1 5 10 15
Pro Val Arg Glu Val Asn Thr Gln Val Lys Gln Trp Ile Glu Arg Leu
20 25 30
Gly His Leu Trp Val Glu Gly Gln Leu Ala Gln Ile Asn Val Lys Pro
35 40 45
Asn Trp Lys Leu Ser Tyr Leu Thr Leu Arg Asp Val Glu Gln Glu Met
50 55 60
Ser Val Gln Leu Thr Cys Pro Thr Asp Ile Ile Arg Asn Arg Pro Thr
65 70 75 80
Pro Leu Lys Asp Gly Asp Arg Val Ile Val Tyr Gly Lys Pro Ala Phe
85 90 95
Tyr Ala Gly Arg Gly Thr Phe Ser Leu Trp Val Thr Asp Ile Arg Pro
100 105 110
Val Gly Ile Gly Glu Leu Leu Ala Arg Ile Glu Glu Leu Arg Lys Arg
115 120 125
Leu Ala Ala Glu Gly Leu Phe Asp Pro Ala Arg Lys Lys Arg Leu Pro
130 135 140
Phe Leu Pro Asn Arg Val Gly Leu Ile Thr Gly Arg Gly Ser Ala Ala
145 150 155 160
Glu Arg Asp Val Leu Ser Val Ala Lys Asp Arg Trp Pro Glu Val Gln
165 170 175
Phe Glu Val Ile Asn Thr Ala Val Gln Gly Ala Ser Ala Val Pro Glu
180 185 190
Ile Ile Glu Ala Leu Arg Val Leu Asp Gln Asp Pro Arg Val Asp Val
195 200 205
Ile Ile Ile Ala Arg Gly Gly Gly Ser Val Glu Asp Leu Leu Pro Phe
210 215 220
Ser Glu Glu Ala Leu Gln Arg Ala Val Ala Ala Ala Gln Thr Pro Val
225 230 235 240
Val Ser Ala Ile Gly His Glu Pro Asp Thr Pro Val Leu Asp Asn Val
245 250 255
Ala Asp Leu Arg Ala Ala Thr Pro Thr Asp Ala Ala Lys Arg Val Val
260 265 270
Pro Asp Val Ala Glu Glu Arg Met Leu Ile Asn Gln Leu Arg Ser Arg
275 280 285
Ser Ala Ala Ala Leu Arg Gly Trp Val Gln Arg Glu Gln Gln Ala Leu
290 295 300
Ala Ala Ile Arg Thr Arg Pro Val Leu Ala Asp Pro Met Thr Pro Ile
305 310 315 320
Asn Arg Arg Arg Asp Glu Ile Ala Gln Ala Val Gly Leu Ile Arg Arg
325 330 335
Asp Val Thr His Leu Val Arg Thr Glu Gln Ala Leu Val Ala Ser Leu
340 345 350
Arg Ala Gln Val Ser Ala Leu Gly Pro Ser Ala Thr Leu Ala Arg Gly
355 360 365
Tyr Ser Val Val Gln Val Ile Pro Arg Asp Gly Ser Ala Pro Glu Val
370 375 380
Val Thr Thr Ile Glu Gln Ser Pro Pro Gly Ser Gln Leu Arg Ile Arg
385 390 395 400
Val Ala Asp Gly Ser Ile Thr Ala Ala Ser Met Gly Thr Gln Gln Ala
405 410 415
Asn
Claims (8)
- 각각 표 1/컬럼 2에 나타낸 아미노산 서열을 갖는 폴리펩티드를 코딩하며, 표 1/컬럼 4에 나타낸 아미노산 위치에서의 핵산 분자가 표 1/컬럼 5의 동일한 열에 나타낸 특정 아미노산과 다른 아미노산을 코딩하는 것인 단리된 핵산 분자.
- 제1항에 있어서, 표 1/컬럼 4에 나타낸 아미노산 위치에서의 핵산 분자가 표 1/컬럼 6의 동일한 열에 나타낸 아미노산을 코딩하는 것인 단리된 핵산 분자.
- 제1항에 청구된 1종 이상의 핵산 서열을 포함하는 벡터.
- 제3항에 청구된 1종 이상의 벡터로 형질감염된 숙주 세포.
- 제4항에 있어서, 상기 핵산 분자의 발현으로 인해 상기 세포로부터의 정밀화학물질 생산이 조절되는 숙주 세포.
- 제3항에 청구된 1종 이상의 벡터로 형질감염된 세포를 배양함으로써 정밀화학물질을 생산하는 것을 포함하는, 정밀화학물질의 생산 방법.
- 제6항에 있어서, 정밀화학물질이 아미노산인 방법.
- 제7항에 있어서, 상기 아미노산이 리신인 방법.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10154180A DE10154180A1 (de) | 2001-11-05 | 2001-11-05 | gene die für genetische Stabilitäts-, genexpressions-und Faltungsproteine codieren |
| DE10154180.5 | 2001-11-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20050042246A true KR20050042246A (ko) | 2005-05-06 |
| KR100861746B1 KR100861746B1 (ko) | 2008-10-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| KR1020047006765A KR100861746B1 (ko) | 2001-11-05 | 2002-10-31 | 유전자 안정성, 유전자 발현 및 폴딩 단백질을 코딩하는유전자 |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US7138513B2 (ko) |
| EP (3) | EP1444258A2 (ko) |
| KR (1) | KR100861746B1 (ko) |
| CN (1) | CN1323087C (ko) |
| AT (1) | ATE429443T1 (ko) |
| AU (1) | AU2002361951A1 (ko) |
| BR (1) | BR0213771A (ko) |
| DE (2) | DE10154180A1 (ko) |
| WO (1) | WO2003040180A2 (ko) |
| ZA (1) | ZA200404424B (ko) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006340603A (ja) * | 2003-06-23 | 2006-12-21 | Ajinomoto Co Inc | Ｌ−グルタミン酸の製造法 |
| DE10359594A1 (de) * | 2003-12-18 | 2005-07-28 | Basf Ag | PEF-TU-Expressionseinheiten |
| DE10359661A1 (de) * | 2003-12-18 | 2005-07-28 | Basf Ag | Genvarianten die für Proteine aus dem Stoffwechselweg von Feinchemikalien codieren |
| DE102004035065A1 (de) | 2004-07-20 | 2006-02-16 | Basf Ag | P-ET-TS-Expressionseinheiten |
| DE102004061846A1 (de) * | 2004-12-22 | 2006-07-13 | Basf Ag | Mehrfachpromotoren |
| DE102005023829A1 (de) | 2005-05-24 | 2006-11-30 | Degussa Ag | Allele des opcA-Gens aus coryneformen Bakterien |
| US20070072194A1 (en) * | 2005-09-28 | 2007-03-29 | Alper Hal S | Global transcription machinery engineering |
| DE102007044134A1 (de) * | 2007-09-15 | 2009-03-19 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| JP7081832B2 (ja) * | 2016-06-13 | 2022-06-07 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | α（Ｖ）β（６）インテグリン結合ペプチドおよびその使用方法 |
| AU2018395054A1 (en) * | 2017-12-29 | 2020-07-16 | The Scripps Research Institute | Unnatural base pair compositions and methods of use |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4649119A (en) | 1983-04-28 | 1987-03-10 | Massachusetts Institute Of Technology | Cloning systems for corynebacterium |
| DE122007000007I2 (de) | 1986-04-09 | 2010-12-30 | Genzyme Corp | Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern |
| US4873316A (en) | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
| DE4120867A1 (de) | 1991-06-25 | 1993-01-07 | Agfa Gevaert Ag | Fotografisches verarbeitungsverfahren und vorrichtung dafuer |
| DE69529026T2 (de) | 1994-07-19 | 2003-07-17 | Hayashibara Biochem Lab | Trehalose, ihre Herstellung und ihre Verwendung |
| DE19929365A1 (de) | 1999-06-25 | 2000-12-28 | Basf Lynx Bioscience Ag | Teilsequenzen der Gene des Primär- und Sekundärmetabolismus aus Corynebacterium glutamicum und ihr Einsatz zur mikrobiellen Herstellung von Primär- und Sekundärmetaboliten |
| MXPA01012844A (es) * | 1999-06-25 | 2002-07-09 | Basf Ag | Genes de corynebacterium glutamicum que codifican proteinas de tolerancia y resistencia al estres. |
| SK3012001A3 (en) * | 1999-07-09 | 2002-07-02 | Degussa | Nucleotide sequences which code for the opca gene and a process for the fermentative preparation of l-amino acids |
| JP4623825B2 (ja) | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
-
2001
- 2001-11-05 DE DE10154180A patent/DE10154180A1/de not_active Withdrawn
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2002
- 2002-10-31 EP EP02796537A patent/EP1444258A2/de not_active Withdrawn
- 2002-10-31 WO PCT/EP2002/012138 patent/WO2003040180A2/de not_active Application Discontinuation
- 2002-10-31 EP EP06110205A patent/EP1693380B1/de not_active Expired - Lifetime
- 2002-10-31 DE DE50213492T patent/DE50213492D1/de not_active Expired - Lifetime
- 2002-10-31 EP EP05026934A patent/EP1669369A2/de not_active Ceased
- 2002-10-31 CN CNB028233409A patent/CN1323087C/zh not_active Expired - Fee Related
- 2002-10-31 AU AU2002361951A patent/AU2002361951A1/en not_active Abandoned
- 2002-10-31 KR KR1020047006765A patent/KR100861746B1/ko not_active IP Right Cessation
- 2002-10-31 US US10/494,541 patent/US7138513B2/en not_active Expired - Fee Related
- 2002-10-31 AT AT06110205T patent/ATE429443T1/de not_active IP Right Cessation
- 2002-10-31 BR BR0213771-2A patent/BR0213771A/pt not_active IP Right Cessation
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2004
- 2004-06-04 ZA ZA200404424A patent/ZA200404424B/xx unknown
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- 2006-10-23 US US11/584,957 patent/US20070037262A1/en not_active Abandoned
- 2006-11-02 US US11/591,868 patent/US7323559B2/en not_active Expired - Fee Related
- 2006-11-03 US US11/592,858 patent/US7339048B2/en not_active Expired - Lifetime
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Also Published As
| Publication number | Publication date |
|---|---|
| US7339048B2 (en) | 2008-03-04 |
| US20050009152A1 (en) | 2005-01-13 |
| WO2003040180A2 (de) | 2003-05-15 |
| US20070037262A1 (en) | 2007-02-15 |
| US20070077631A1 (en) | 2007-04-05 |
| KR100861746B1 (ko) | 2008-10-29 |
| ATE429443T1 (de) | 2009-05-15 |
| CN1582299A (zh) | 2005-02-16 |
| EP1693380A2 (de) | 2006-08-23 |
| ZA200404424B (en) | 2005-06-06 |
| EP1444258A2 (de) | 2004-08-11 |
| CN1323087C (zh) | 2007-06-27 |
| US7355028B2 (en) | 2008-04-08 |
| WO2003040180A3 (de) | 2004-04-01 |
| DE50213492D1 (de) | 2009-06-04 |
| BR0213771A (pt) | 2004-10-19 |
| EP1669369A2 (de) | 2006-06-14 |
| DE10154180A1 (de) | 2003-05-15 |
| EP1693380B1 (de) | 2009-04-22 |
| EP1693380A3 (de) | 2007-01-03 |
| US20070054381A1 (en) | 2007-03-08 |
| US7138513B2 (en) | 2006-11-21 |
| US7323559B2 (en) | 2008-01-29 |
| US20070072273A1 (en) | 2007-03-29 |
| AU2002361951A1 (en) | 2003-05-19 |
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