CN111979164A - 一种产l-赖氨酸的重组菌株及其构建方法与应用 - Google Patents
一种产l-赖氨酸的重组菌株及其构建方法与应用 Download PDFInfo
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- CN111979164A CN111979164A CN202010790868.1A CN202010790868A CN111979164A CN 111979164 A CN111979164 A CN 111979164A CN 202010790868 A CN202010790868 A CN 202010790868A CN 111979164 A CN111979164 A CN 111979164A
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- seq
- ncgl1089
- sequence
- polynucleotide
- lysine
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
成分 | 配方 |
蔗糖 | 10g/L |
多聚蛋白胨 | 10g/L |
牛肉膏 | 10g/L |
酵母粉 | 5g/L |
尿素 | 2g/L |
氯化钠 | 2.5g/L |
琼脂粉 | 20g/L |
pH | 7.0 |
培养温度 | 32度 |
成分 | 配方 |
淀粉水解糖 | 30g/L |
硫酸铵 | 12g/L |
硫酸镁 | 0.87g/L |
糖蜜 | 20g/L |
酸化玉米浆 | 3mL/L |
磷酸 | 0.4mL/L |
氯化钾 | 0.53g/L |
消泡剂(2%泡敌) | 4mL/L |
硫酸亚铁 | 120mg/L |
硫酸锰 | 120mg/L |
烟酰胺 | 42mg/L |
泛酸钙 | 6.3mg/L |
维生素B1 | 6.3mg/L |
铜、锌盐溶液 | 0.6g/L |
生物素 | 0.88mg/L |
菌株 | L-赖氨酸产量(g/100ml) | OD(660nm) |
YP97158 | 18.8 | 37.5 |
YPL-4-017 | 19.1 | 37.3 |
YPL-4-018 | 19.3 | 37.6 |
YPL-4-019 | 19.5 | 37.8 |
YPL-4-020 | 19.4 | 37.2 |
YPL-4-021 | 19.8 | 36.5 |
YPL-4-022 | 18.0 | 37.7 |
Claims (10)
Priority Applications (6)
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CN202010790868.1A CN111979164B (zh) | 2020-08-07 | 2020-08-07 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
US18/040,883 US20230323412A1 (en) | 2020-08-07 | 2021-01-04 | Recombinant strain for producing l-amino acid and construction method and use thereof |
PCT/CN2021/070190 WO2022027924A1 (zh) | 2020-08-07 | 2021-01-04 | 一种产l-氨基酸的重组菌株及其构建方法与应用 |
JP2023507248A JP2023536298A (ja) | 2020-08-07 | 2021-01-04 | L-アミノ酸を生産する組換え菌株、その構築方法及び使用 |
KR1020237007352A KR20230045051A (ko) | 2020-08-07 | 2021-01-04 | L-아미노산을 생산하는 재조합 균주 및 이의 구축방법과 응용 |
EP21854280.1A EP4194545A1 (en) | 2020-08-07 | 2021-01-04 | Recombinant strain for producing l-amino acid, and construction method therefor and use thereof |
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CN202010790868.1A CN111979164B (zh) | 2020-08-07 | 2020-08-07 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111979165A (zh) * | 2020-08-07 | 2020-11-24 | 黑龙江伊品生物科技有限公司 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
CN112522175A (zh) * | 2020-12-30 | 2021-03-19 | 内蒙古伊品生物科技有限公司 | 一种改造基因bbd29_09525产l-谷氨酸的重组菌株及其构建方法与应用 |
CN112625992A (zh) * | 2020-12-30 | 2021-04-09 | 宁夏伊品生物科技股份有限公司 | 一种改造基因bbd29_11265产l-谷氨酸的重组菌株及其构建方法与应用 |
CN112980867A (zh) * | 2021-03-09 | 2021-06-18 | 中国科学院深圳先进技术研究院 | 一种改造谷氨酸棒杆菌启动子的重组菌株及其构建方法与产l-氨基酸的应用 |
WO2022027924A1 (zh) * | 2020-08-07 | 2022-02-10 | 宁夏伊品生物科技股份有限公司 | 一种产l-氨基酸的重组菌株及其构建方法与应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1582300A (zh) * | 2001-11-05 | 2005-02-16 | 巴斯福股份公司 | 编码新蛋白的基因 |
US6962989B1 (en) * | 1999-07-08 | 2005-11-08 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding novel proteins |
CN106367432A (zh) * | 2016-09-01 | 2017-02-01 | 宁夏伊品生物科技股份有限公司 | 发酵生产l‑赖氨酸的方法及改造的棒杆菌 |
-
2020
- 2020-08-07 CN CN202010790868.1A patent/CN111979164B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6962989B1 (en) * | 1999-07-08 | 2005-11-08 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding novel proteins |
CN1582300A (zh) * | 2001-11-05 | 2005-02-16 | 巴斯福股份公司 | 编码新蛋白的基因 |
CN106367432A (zh) * | 2016-09-01 | 2017-02-01 | 宁夏伊品生物科技股份有限公司 | 发酵生产l‑赖氨酸的方法及改造的棒杆菌 |
Non-Patent Citations (3)
Title |
---|
WP_011014143.1: "(2Fe-2S)-binding protein [Corynebacterium glutamicum]", 《GENBANK》 * |
张惠展 编著: "《基因工程(第4版)》", 31 January 2017, 华东理工大学出版社 * |
苏会波 等人: "谷氨酸棒杆菌生产L-赖氨酸的培养优化和产酸特性研究", 《食品工业科技》 * |
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CN111979165B (zh) * | 2020-08-07 | 2021-05-07 | 黑龙江伊品生物科技有限公司 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
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