KR100868693B1 - 대사 경로 단백질을 코딩하는 유전자 - Google Patents

Abstract

본 발명은 신규한 핵산 분자, 생명공학적으로 개선된 미생물 제조에 있어서의 그의 용도 및 상기 생명공학적으로 개선된 미생물을 사용한 정밀 화학물질, 특히 아미노산의 제조 방법에 관한 것이다.

핵산 분자, 미생물, 대사 경로, 유전자, 정밀 화학물질, 아미노산

Description

대사 경로 단백질을 코딩하는 유전자 {Genes Coding for Metabolic Pathway Proteins}

세포에서 자연 발생적 대사 과정의 특정 생성물 및 부산물은 식품 산업, 동물 사료 산업, 화장품 산업 및 제약 산업을 비롯한 다양한 산업 분야에 이용된다. 총체적으로 "정밀 화학물질 (fine chemical)"로 언급되는 이들 분자에는 유기산, 단백질생성 및 비-단백질생성 아미노산, 뉴클레오티드 및 뉴클레오시드, 지질 및 지방산, 디올, 탄수화물, 방향족 화합물, 비타민 및 보조인자, 및 효소가 포함된다. 이들은 1종 이상의 관심 분자를 대량으로 생산하고 분비하도록 개발된 박테리아의 대규모 배양을 통해 가장 우수하게 생산된다. 이러한 목적에 특히 유용한 유기체는 그람 양성의 비병원성 박테리아인 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum)이다. 균주 선별을 통해, 각종 바람직한 화합물을 생산하는 다수의 돌연변이 균주가 개발되어 있다. 그러나, 특정 분자 생산의 개선을 위한 균주 선별은 시간 소모적이고 어려운 과정이다.

<발명의 개요>

본 발명은 코리네박테리움 글루타미쿰 또는 관련 박테리아 종을 확인하거나 분류하기 위해 사용할 수 있는 신규 핵산 분자를 제공한다. 코리네박테리움 글루타미쿰은 수많은 정밀 화학물질의 대규모 생산, 탄화수소의 분해 (예를 들어, 원유 스필의 경우) 및 테르페노이드의 산화를 위해 산업적으로 널리 사용되는 그람 양성 호기성 박테리아이다. 따라서, 상기 핵산 분자는 발효 공정 등에 의한 정밀 화학물질 생산에 이용할 수 있는 미생물 확인에 사용할 수 있다. 코리네박테리움 글루타미쿰 그 자체는 인간에게 병원성이 아니지만, 코리네박테리움 디프테리애 (Corynebacterium diphtheriae) (디프테리아의 원인균)와 같이, 인간에게 주요한 병원성인 종과 관련되어 있다. 그러므로, 코리네박테리움 종의 존재 확인 능력은 또한 임상적으로, 예를 들어 진단 적용에 있어 상당히 중요할 수도 있다. 추가로, 상기 핵산 분자는 코리네박테리움 글루타미쿰의 게놈 또는 관련 유기체의 게놈을 맵핑하기 위한 기준점으로 작용할 수 있다.

이들 신규 핵산 분자는 본원에서 대사 경로 (MP) 단백질로 지칭된 단백질을 코딩한다. 이들 MP 단백질은 예를 들어 세포의 정상적인 대사 기능에 결정적인 단백질의 전사, 번역 또는 후번역 조절에 관련된 기능을 발휘할 수 있다. 예를 들어 미국 특허 제4,649,119호 [Sinskey et al.]에 개시된 클로닝 벡터와 같은 코리네박테리움 글루타미쿰에 사용하기 위한 클로닝 벡터, 및 관련된 브레비박테리움 종 (예를 들어, 락토페르멘툼)의 유전자 조작 기술 (문헌 [Yoshihama et al, J. Bacteriol. 162: 591-597 (1985)], 문헌 [Katsumata et al., J. Bacteriol. 159: 306-311 (1984)], 및 문헌 [Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984)])을 이용하여 본 발명의 핵산 분자를 이용하여 이 유기체를 유전공학적으로 조작함으로써, 이 유기체가 1종 이상의 정밀 화학물질의 보다 우수하거나 보다 효율적인 생산자가 되도록 할 수 있다.

본 발명의 유전자를 조작함으로써 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율을 직접적으로 또는 간접적으로 개선시킬 수 있다. 더욱 구체적으로, 보통 정밀 화학물질 대사 경로 중 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율을 조절하는 코리네박테리움 글루타미쿰 MP 단백질의 변형은 상기 유기체로부터의 1종 이상의 상기 원하는 화합물의 총생산 또는 생산 속도에 직접적인 영향을 줄 수 있다. 이들 대사 경로에 관여하는 상기 단백질의 변형은 또한 원하는 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율에 간접적인 영향을 줄 수도 있다. 대사 조절은 반드시 복잡하며, 여러가지 경로에 영향을 주는 조절 메카니즘은 많은 장소에서 중복될 수 있으므로, 1개 이상의 대사 경로는 특정 세포내 사건에 따라 신속하게 조정될 수 있다. 이로 인해 1개의 대사 경로에 대한 조절 단백질의 변형이 수많은 다른 대사 경로 (그 중 일부는 관심 정밀 화학물질의 생합성 또는 분해에 관여할 수 있음)에 영향을 주는 것이 가능해진다. 이러한 간접적인 방식으로, MP 단백질의 작용 조정은 상기 MP 단백질에 의해 직접적으로 조절되는 대사 경로와 상이한 대사 경로를 통해 생산된 정밀 화학물질의 생산에 영향을 줄 수 있다.

본 발명의 핵산 및 단백질 분자를 이용하여, 코리네박테리움 글루타미쿰으로부터 1종 이상의 관심 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율을 직접 개선시킬 수 있다. 당업계에 공지된 유전자 재조합 기술을 이용하여, 본 발명의 1종 이상의 조절 단백질을 조작하여, 그 기능을 조절할 수 있다. 아미노산 생합성에 요구되는 효소를 코딩하는 유전자의 전사를 억제하는 데에 관여하는 MP 단백질의 돌연변이 (여기서 상기 단백질은 더이상 전사를 억제할 수 없음)로 인해, 예를 들어, 상기 아미노산의 생산이 증가될 수 있다. 따라서, 번역 증가를 유발하거나 관심 정밀 화학물질의 생합성에 관여하는 코리네박테리움 글루타미쿰 단백질의 후번역 변형을 활성화시키는 MP 단백질의 활성이 변형되면, 상기 화학물질의 생산이 차례로 증가될 수 있다. 반대의 상황도 또한 유용할 수 있다: 전사 또는 번역의 억제를 증가시키거나, 화합물의 분해 경로 조절에 관여하는 코리네박테리움 글루타미쿰 단백질을 후번역적으로 음으로 변형시킴으로써, 상기 화학물질의 생산을 증가시키는 것이 가능하다. 각 경우에서, 관심 정밀 화학물질의 총 수율 또는 생산 속도가 증가될 수 있다.

또한, 본 발명의 단백질 및 뉴클레오티드 분자에서의 이러한 변형으로 간접적 메카니즘을 통해 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율이 개선될 수 있는 것이 가능하다. 특정 화합물의 대사는 세포내 다른 생합성 및 분해 경로와 반드시 연결되어 있고, 한 대사 경로에 필요한 보조인자, 중간체 또는 기질은 또다른 대사 경로에 의해 공급되거나 제한되는 것으로 보인다. 그러므로, 본 발명의 1종 이상의 조절 단백질을 조정하여, 정밀 화학물질의 다른 생합성 또는 분해 경로의 활성 효율에 영향을 미칠 수 있다. 이에 더하여, 배양에서, 특히 성장 조건이 최적에 이르지 못할 수 있는 대규모 발효 배양에서, 1종 이상의 조절 단백질을 조작하여 세포가 생장 및 번식하는 전반적인 능력을 증가시킬 수 있다. 예를 들어, 최적에 이르지 못하는 영양소의 세포외 공급 (그로 인한 세포 분열 저지)으로의 반응으로서 뉴클레오시드의 생합성을 보통 억제하는 본 발명의 MP 단백질을 돌연변이시킴으로써 (이 때 상기 단백질은 낮은 억제자 활성을 갖음) 뉴클레오시드 의 생합성 및 아마도 세포 분열을 증가시키는 것이 가능하다. 배양 중 세포 성장 증가 및 세포 분열 증가를 유발하는 상기 MP 단백질을 변형시키는 것은 적어도 배양 중에 관심 정밀 화학물질을 생산하는 세포의 수를 증가시킴으로써, 배양물로부터의 1종 이상의 상기 화학물질의 수율, 생산량 및(또는) 생산 효율 증가를 야기할 수 있다.

본 발명은 예를 들어 코리네박테리움 글루타미쿰에서 대사 경로의 전사, 번역 또는 후번역 조절에 관여하는 효소 단계를 수행할 수 있으며, 본원에서 대사 조절 (MP) 단백질로 언급되는 단백질을 코딩하는 신규 핵산 분자를 제공한다. MP 단백질을 코딩하는 핵산 분자는 본원에서 MP 핵산 분자로 언급된다. 바람직한 실시양태에서, MP 단백질은 하나 이상의 대사 경로의 전사, 번역 또는 후번역 조절에 관여한다. 이러한 단백질의 예로는 표 1에 기재된 유전자에 의해 코딩되는 단백질을 들 수 있다.

따라서, 본 발명의 한 측면은 MP 단백질 또는 생물학적으로 활성인 그의 절편을 코딩하는 뉴클레오티드 서열을 포함하는 단리된 핵산 분자 (예를 들어, cDNA) 뿐만 아니라 MP 코딩 핵산 (예를 들어, DNA 또는 mRNA)의 검출 또는 증폭을 위한 프라이머 또는 혼성화 프로브로서 적합한 핵산 단편에 관한 것이다. 특히 바람직한 실시양태에서, 단리된 핵산 분자는 첨부문서 A에 기재된 임의의 뉴클레오티드 서열, 또는 이들 임의의 뉴클레오티드 서열의 코딩 영역 또는 그의 상보체를 포함한다. 다른 바람직한 실시양태에서, 단리된 핵산 분자는 첨부문서 B에 기재된 임의의 아미노산 서열을 코딩한다. 본 발명의 바람직한 MP 단백질은 본원에 기재된 MP 활성 중 적어도 하나를 갖는 것도 바람직하다.

첨부문서 A는 하기에서 표 1에 기재된 해당 위치에서의 서열 변형과 함께 서열 목록의 핵산 서열을 규정한다.

첨부문서 B는 하기에서 표 1에 기재된 해당 위치에서의 서열 변형과 함께 서열 목록의 핵산 서열을 규정한다.

추가의 실시양태에서, 단리된 핵산 분자는 그 길이가 뉴클레오티드 15개 이상이고 첨부문서 A의 뉴클레오티드 서열을 포함하는 핵산 분자와 엄격 조건하에서 혼성화한다. 바람직하게는, 단리된 핵산 분자는 자연 발생 핵산 분자에 상응한다. 더욱 바람직하게는, 단리된 핵산은 코리네박테리움 글루타미쿰의 MP 단백질 또는 그의 생물학적 활성 절편을 코딩한다.

본 발명의 다른 측면은 본 발명의 핵산 분자를 함유하는 벡터, 예를 들어, 재조합 발현 벡터, 및 이러한 벡터가 도입된 숙주 세포에 관한 것이다. 한 실시양태에서, 적당한 배지에서 배양된 숙주 세포를 이용하여 MP 단백질을 생산한다. 이어서, MP 단백질을 배지 또는 숙주 세포로부터 단리할 수 있다.

본 발명의 다른 측면은 MP 유전자가 도입되거나 변형되어 유전적으로 변형된 미생물에 관한 것이다. 한 실시양태에서, 상기 미생물의 게놈은 돌연변이된 MP 서열을 코딩하는 1종 이상의 본 발명의 핵산 분자를 트랜스진으로서 도입함으로써 변형된다. 다른 실시양태에서, 상기 미생물 게놈 내의 내인성 MP 유전자는 변형된 MP 유전자와의 상동성 재조합에 의해 변형되는데, 예를 들면 기능적으로 파괴된다. 바람직한 실시양태에서, 상기 미생물은 코리네박테리움 또는 브레비박테리움 속에 포함되는데, 코리네박테리움 글루타미쿰인 것이 특히 바람직하다. 바람직한 실시양태에서, 상기 미생물은 아미노산, 특히 바람직하게는 리신과 같은 관심 화합물의 생산에도 사용된다.

또다른 바람직한 실시양태는 첨부문서 A에 기재된 1종 초과의 핵산 분자를 갖는 숙주 세포이다. 이러한 숙주 세포는 당업자에게 공지된 각종 방법으로 제조할 수 있다. 이는 예를 들어 여러개의 본 발명의 핵산 분자를 운반하는 벡터에 의해 형질감염될 수 있다. 그러나, 각 경우에 한개의 본 발명의 핵산 분자를 숙주 세포로 도입하기 위해 벡터를 이용하고, 이로써 복수개의 벡터를 동시에 또는 순차적으로 사용하는 것도 가능하다. 따라서, 수백개 이하의 수많은 본 발명의 핵산 서열을 운반하는 숙주 세포를 제작하는 것이 가능하다. 이러한 축적은 종종 정밀 화학물질의 생산성 면에서 숙주 세포에 초부가적인 효과를 줄 수 있다.

본 발명의 다른 측면은 단리된 MP 단백질 또는 그의 절편, 예를 들어 그의 생물학적 활성 절편에 관한 것이다. 바람직한 실시양태에서, 단리된 MP 단백질 또는 그의 절편은 코리네박테리움 글루타미쿰에서의 하나 이상의 대사 경로를 전사, 번역 또는 후번역적으로 조절한다. 다른 바람직한 실시양태에서, 단리된 MP 단백질 또는 그의 절편은 상기 단백질 또는 그의 절편이 코리네박테리움 글루타미쿰에서의 하나 이상의 대사 경로를 전사, 번역 또는 후번역적으로 여전히 조절할 수 있을 만큼 첨부문서 B의 아미노산 서열과 충분한 상동성이 있다.

본 발명은 단리된 MP 단백질의 제제를 제공한다. 바람직한 실시양태에서, MP 단백질은 첨부문서 B의 아미노산 서열을 포함한다. 추가의 바람직한 실시양태 에서, 본 발명은 첨부문서 B의 전체 아미노산 서열 (이는 첨부문서 A에서 오픈 리딩 프레임에 의해 코딩됨)과 본질적으로 상동성이 있는 단리된 전장 단백질에 관한 것이다.

MP 폴리펩티드 또는 그의 생물학적 활성 절편을 MP 폴리펩티드가 아닌 폴리펩티드와 기능적으로 연결하여 융합 단백질을 형성할 수 있다. 바람직한 실시양태에서, 이 융합 단백질의 활성은 MP 단백질만의 활성과 다르고, 다른 바람직한 실시양태에서, 코리네박테리움 글루타미쿰에서의 하나 이상의 대사 경로를 전사, 번역 또는 후번역적으로 조절한다. 특히 바람직한 실시양태에서, 이 융합 단백질을 숙주 세포로 통합시켜 이 세포에 의해 관심 화합물이 생산되는 것을 조정한다.

본 발명의 다른 측면은 정밀 화학물질의 제조 방법에 관한 것이다. 이 방법은 본 발명의 MP 핵산 분자의 발현을 유발하는 벡터를 함유하는 세포를 배양하여 정밀 화학물질을 생산하는 것을 제공한다. 바람직한 실시양태에서, 이 방법은 MP 핵산의 발현을 유발하는 벡터로 세포를 형질감염시켜 상기 벡터를 함유하는 세포를 수득하는 단계를 추가로 포함한다. 다른 바람직한 실시양태에서, 이 방법은 배양물로부터 정밀 화학물질을 수득하는 단계를 추가로 포함한다. 바람직한 실시양태에서, 상기 세포는 코리네박테리움 또는 브레비박테리움 속에 속한다.

본 발명의 다른 측면은 미생물로부터 분자가 생산되는 것을 조정하는 방법에 관한 것이다. 이러한 방법은 MP 단백질 활성 또는 MP 핵산 발현을 조정하는 물질과 세포를 접촉시켰을 때의 세포 관련 활성이 상기 물질의 부재 하에서의 동일한 활성에 비해 변형되도록 하는 것을 포함한다. 바람직한 실시양태에서, 코리네박테 리움 글루타미쿰에서의 대사 경로에 대한 하나 이상의 조절 시스템에 대해 세포를 조정하여, 이 미생물에 의한 관심 정밀 화학물질의 생산 속도 또는 수율을 개선시킨다. MP 단백질 활성을 조절하는 물질은, 예를 들어 MP 단백질 활성 또는 MP 핵산 발현을 자극한다. MP 단백질 활성 또는 MP 핵산 발현을 자극하는 물질의 예로는 소분자, 활성 MP 단백질, 및 세포로 도입되어 MP 단백질을 코딩하는 핵산이 있다. MP 활성 또는 발현을 억제하는 물질의 예로는 소분자 및 안티센스 MP 핵산 분자 등이 있다.

본 발명의 다른 측면은 MP 야생형 유전자 또는 MP 돌연변이 유전자가 별개의 플라스미드 상에 유지되거나 숙주 세포의 게놈 내로 통합되도록 이 유전자를 세포에 도입하는 것을 포함하는, 세포로부터의 관심 화합물의 수율을 조정하는 방법에 관한 것이다. 게놈 내로의 통합은 무작위적일 수도 있고 통합된 카피가 천연 유전자를 대체하도록 하는 상동성 재조합에 의해 일어날 수도 있는데, 이러한 통합에 의해 세포로부터의 관심 화합물의 생산이 조정된다. 바람직한 실시양태에서, 상기 수율은 증가한다. 다른 바람직한 실시양태에서, 상기 화학물질은 정밀 화학물질이고, 특히 바람직한 실시양태에서, 이는 아미노산이다. 특히 바람직한 실시양태에서, 상기 아미노산은 L-리신이다.

본 발명은 정밀 화학물질 대사의 조절을 비롯한 코리네박테리움 글루타미쿰 대사의 조절에 관여하는 MP 핵산 및 MP 단백질 분자를 제공한다. 본 발명의 분자를 이용하여 코리네박테리움 글루타미쿰 등의 미생물로부터의 정밀 화학물질 생산 을 직접적으로 (예를 들어, 리신 대사 경로의 조절 단백질의 활성 조정은 이 유기체로부터의 리신 수율, 생산량 및(또는) 생산 효율에 직접적인 영향을 줌), 또는 역시 관심 화합물의 수율, 생산량 및(또는) 생산 효율의 증가를 초래하면서 간접적으로 (예를 들어, 뉴클레오티드 생합성 단백질 조절의 조정은 이 박테리아로부터의 유기산 또는 지방산 생산에 영향을 주는데, 이는 뉴클레오티드 생합성의 변형된 조절로의 반응으로서 상기 화학물질에 대한 생합성 또는 분해 경로에서의 변형된 조절을 동반하기 때문으로 보임) 조정할 수 있다. 본 발명의 측면은 하기에서 추가로 예시된다.

I. 정밀 화학물질

용어 "정밀 화학물질"은 당업계에 공지되어 있고, 제약, 농업 및 화장품 산업과 같은, 그러나 이에 제한되지 않는 다양한 산업에 이용되는 유기체에 의해 생산되는 분자를 포함한다. 이러한 화합물로는 타르타르산, 이타콘산 및 디아미노피멜산과 같은 유기산, 단백질생성 및 비-단백질생성 아미노산, 퓨린 및 피리미딘 염기, 뉴클레오시드, 및 뉴클레오티드 (예를 들어, 문헌 [Kuninaka, A. (1996) Nucleotides and related compounds, p.561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim]과 그의 참고문헌에 기재된 바와 같음), 지질, 포화 및 불포화 지방산 (예를 들어, 아라키돈산), 디올 (예를 들어, 프로판디올 및 부탄디올), 탄수화물 (예를 들어, 히알루론산 및 트레할로스), 방향족 화합물 (예를 들어 방향족 아민, 바닐린 및 인디고), 비타민 및 보조인자 (문헌 [Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p.443-613 (1996) VCH: Weinheim] 및 그의 참고문헌, 및 문헌 [Ong, A.S., Niki, E. ＆ Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3,1994 at Penang, Malaysia, AOCS Press, (1995)]에 기재된 바와 같음), 효소 및 문헌 [Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086] 및 상기 문헌에 표시된 참고문헌에 기재된 다른 모든 화학물질이 있다. 특정 정밀 화학물질의 대사 및 용도는 아래에 추가로 예시된다.

A. 아미노산 대사 및 용도

아미노산은 모든 단백질의 기본 구조 단위를 구성하므로 정상적인 세포 기능에 필수적이다. 용어 "아미노산"은 당업계에 공지되어 있다. 아미노산 중 단백질생성 아미노산은 20종이며 펩티드 결합으로 연결되어 있는 단백질의 구조 단위로 작용하는 반면, 비-단백질생성 아미노산 (수백 종이 공지되어 있음)은 정상적으로는 단백질에서 발견되지 않는다 (문헌 [Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p.57-97 VCH: Weinheim (1985)] 참조). L-아미노산이 통상적으로 자연 발생 단백질에서 발견되는 유일한 형태이지만, 아미노산은 D-광학 또는 L-광학 구조일 수 있다. 20종의 단백질생성 아미노산 각각의 생합성 및 분해 경로의 특징은 원핵세포 및 진핵세포 둘다에서 잘 규명되어 있다 (예를 들어, 문헌 [Stryer, L. Biochemistry, 3rd edition, pages 578-590 (1988)] 참조). 생합성의 복잡성으로 인한 일반적인 영양 요구물이기 때문에 "필수" 아미노산이라고 지칭되 는 아미노산 (히스티딘, 이소루이신, 루이신, 리신, 메티오닌, 페닐알라닌, 트레오닌, 트립토판 및 발린)은 단순한 생합성 경로에 의해 나머지 11종의 "비필수" 아미노산 (알라닌, 아르기닌, 아스파라진, 아스파르테이트, 시스테인, 글루타메이트, 글루타민, 글리신, 프롤린, 세린 및 티로신)으로 쉽게 전환된다. 고등동물은 필수 아미노산 중 일부를 합성하는 능력을 보유하지만, 상기 필수 아미노산은 식사로부터 공급되어야 정상적인 단백질 합성이 일어난다.

아미노산의 단백질 생합성에서의 기능 이외에도, 이들 아미노산은 그 자체가 흥미로운 화학물질이고, 다수의 아미노산이 식품, 사료, 화학물질, 화장품, 농업 및 제약 산업에 다양하게 사용될 수 있는 것으로 밝혀져 있다. 리신은 인간 뿐만 아니라 가금 및 돼지와 같은 단위 (monogastric) 동물의 영양에 있어서도 중요한 아미노산이다. 글루타메이트는 향미제 첨가물 (모노-소듐 글루타메이트, MSG)로서 가장 흔히 사용되고, 아스파르테이트, 페닐알라닌, 글리신 및 시트테인도 식품 산업 전반에 걸쳐 광범위하게 사용된다. 글리신, L-메티오닌 및 트립토판은 모두 제약 산업에 사용된다. 글루타민, 발린, 루이신, 이소루이신, 히스티딘, 아르기닌, 프롤린, 세린 및 알라닌은 제약 및 화장품 산업 둘다에 사용된다. 트레오닌, 트립토판 및 D/L-메티오닌은 통상적인 사료 첨가제이다 (문헌 [Leuchtenberger, W. (1996) Amino acids technical production and use, p.466-502 in Rehm et al. (eds.) Biotechnology vol. 6, chapter 14a, VCH: Weinheim]). 또한, 이들 아미노산은 합성 아미노산 및 단백질, 예를 들어, N-아세틸시스테인, S-카르복시메틸-L-시스테인, (S)-5-히드록시트립토판, 및 문헌 [Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p.57-97, VCH: Weinheim, 1985]에 기재된 다른 것의 합성을 위한 전구체로 추가로 적합하다는 것이 밝혀져 있다.

박테리아 등과 같이 이들 천연 아미노산을 생산할 수 있는 유기체에서의 이들 천연 아미노산의 생합성 특징은 잘 규명되어 있다 (박테리아 아미노산 생합성 및 그의 조절에 대한 자세한 것은 문헌 [Umbarger, H.E. (1978) Ann. Rev. Biochem. 47: 533-606] 참조). 글루타메이트는 시트르산 사이클의 중간체인 α-케토글루타레이트의 환원성 아미노화에 의해 합성된다. 글루타민, 프롤린 및 아르기닌은 각각 글루타메이트로부터 생성된다. 세린의 생합성은 3-포스포글리세레이트 (해당작용의 중간체)로 출발하여, 산화, 트랜스아미노화 및 가수분해의 3 단계 과정 후에 세린이 생성된다. 시스테인 및 글리신은 둘다 세린으로부터 생성되는데, 시스테인은 호모시스테인과 세린의 축합에 의해 생성되며, 글리신은 세린 트랜스히드록시메틸라제에 의해 촉매되는 반응에서 측쇄 β-탄소 원자가 테트라히드로폴레이트로 전달되어 생성된다. 페닐알라닌 및 티로신은 프레페네이트의 합성 후 최종 두 단계에서만 다른 9 단계 생합성 경로에서 해당작용 및 펜토스 포스페이트 경로 전구체인 에리쓰로스 4-포스페이트 및 포스포에놀피루베이트로부터 합성된다. 트립토판도 이들 두 초기 분자로부터 합성되지만, 그의 합성은 11 단계 경로이다. 티로신은 페닐알라닌 히드록실라제에 의해 촉매되는 반응에서 페닐알라닌으로부터 합성될 수도 있다. 알라닌, 발린 및 루이신은 해당작용의 최종 생성물인 피루베이트의 모든 생합성 생성물이다. 아스파르테이트는 시트르산 사이클의 중간체인 옥살로아세테이트로부터 형성된다. 아스파라진, 메티오닌, 트레오닌 및 리신 각각은 아스파르테이트의 전환에 의해 생성된다. 이소루이신은 트레오닌으로부터 형성된다. 복잡한 9 단계 경로에서 활성화된 당인 5-포스포리보실-1-피로포스페이트로부터 히스티딘이 생성된다.

세포의 단백질 합성 필요량을 초과하는 아미노산은 저장될 수 없고, 그 대신에 분해되어 세포의 주요 대사 경로에 대한 중간체로 제공된다 (자세한 것은 문헌 [Stryer, L. Biochemistry 3rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p.495-516 (1988)] 참조). 세포가 불필요한 아미노산을 유용한 대사 중간체로 전환시킬 수 있다 하더라도, 아미노산 생성은 에너지, 전구체 분자, 및 이들 아미노산을 합성하는 데 필요한 효소를 고려할 때 손실이 큰 합성이다. 따라서, 특정한 아미노산의 존재가 그 자신의 생성을 서서히 또는 완전히 중지시키는 피드백 억제에 의해 아미노산의 생합성이 조절된다는 것은 놀라운 일이 아니다 (아미노산 생합성 경로의 피드백 메카니즘에 대한 전체적인 내용은 문헌 [Stryer, L. Biochemistry 3rd ed. Ch. 24 "Biosynthesis of Amino Acids and Heme" p. 575-600 (1988)] 참조). 따라서, 임의의 특정 아미노산의 생산량은 세포에 존재하는 아미노산의 양에 의해 제한된다.

B. 비타민, 보조인자 및 영양제의 대사 및 용도

비타민, 보조인자 및 영양제는 분자의 다른 군을 구성한다. 이들은 박테리아와 같은 다른 유기체에 의해 쉽게 합성된다 하더라도 고등동물이 합성 능력을 상실했기 때문에 섭취해야 한다. 이들 분자는 그 자체가 생활성 물질이거나, 다양한 대사 경로에서 전자 전달자 또는 중간체로 작용할 수 있는 생물학적 활성 물질의 전구체이다. 이들 화합물은 그 영양적 가치 뿐 아니라, 색소, 항산화제 및 촉매로서, 또는 다른 과정의 보조제로서도 상당한 산업적 가치를 갖는다 (이들 화합물의 구조, 활성 및 산업적인 용도에 대한 것은 예를 들어, 문헌 [Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p.443-613, VCH: Weinheim, 1996] 참조). 용어 "비타민"은 당업계에 공지되어 있고, 유기체의 정상적인 기능을 위해서는 필요하지만 유기체가 스스로 합성할 수 없는 영양분을 포함한다. 비타민 군은 보조인자 및 영양제 화합물도 포함할 수 있다. 용어 "보조인자"는 정상적인 효소 활성이 일어나는 데 필요한 비-단백질생성 화합물을 포함한다. 이러한 화합물은 유기성 또는 무기성일 수 있고, 본 발명의 보조인자 분자는 유기성인 것이 바람직하다. 용어 "영양제 (nutraceutical)"는 식물 및 동물, 특히 인간에게 건강상 유익한 식이성 보충물을 포함한다. 이러한 분자의 예로는 비타민, 항산화제 및 일부 지질 (예를 들어, 다불포화 지방산)이 있다.

이들을 생산할 수 있는 유기체, 예를 들어, 박테리아에서 일어나는 이들 분자의 생합성의 특징은 잘 규명되어 있다 (문헌 [Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p.443-613, VCH: Weinheim, 1996], 문헌 [Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley ＆ Sons], 문헌 [Ong, A.S., Niki, E. ＆ Packer, L. (1995) "Nutrition, Lipids, Health, and Disease "Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research-Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S]).

티아민 (비타민 B₁)은 피리미딘과 티아졸 잔기의 화학적 커플링에 의해 생성된다. 리보플라빈 (비타민 B₂)는 구아노신-5'-트리포스페이트 (GTP) 및 리보스-5'-포스페이트로부터 합성된다. 그 다음, 상기 리보플라빈은 플라빈 모노뉴클레오티드 (FMN) 및 플라빈 아데닌 디뉴클레오티드 (FAD)의 합성에 사용된다. 총칭하여 "비타민 B₆"로 지칭되는 화합물 족 (예를 들어, 피리독신, 피리독사민, 피리독사-5'-포스페이트 및 시판되는 피리독신 히드로클로라이드)은 모두 통상적인 구조 단위인 5-히드록시-6-메틸피리딘의 유도체이다. 판토테네이트 (판토텐산, (R)-(+)-N-(2,4-디히드록시-3,3-디메틸-1-옥소부틸)-β-알라닌)은 화학적인 합성 또는 발효에 의해 생성될 수 있다. 판토테네이트의 생합성의 최종 단계는 β-알라닌과 판토산의 ATP-유도 축합으로 구성된다. 판토산으로 전환하는 생합성 단계, β-알라닌으로 전환하는 생합성 단계 및 판토텐산으로의 축합에 관여하는 효소는 공지되어 있다. 판토테네이트의 대사 활성 형태는 보조효소 A인데, 이 보조효소 A를 위한 생합성은 5 단계 효소 반응으로 진행된다. 판토테네이트, 피리독살-5'-포스페이트, 시스테인 및 ATP는 보조효소 A의 전구체이다. 이들 효소는 판토테네이트의 형성을 촉매할 뿐만 아니라 (R)-판토산, (R)-판토락톤, (R)-판테놀 (프로비타민 B₅), 판테테인 (및 그의 유도체) 및 보조효소 A의 생성도 촉매한다.

미생물에서 전구체 분자인 피멜로일-CoA로부터의 비오틴 생합성 과정은 자세히 연구되어 있고 이에 관련된 여러가지 유전자가 확인되어 있다. 상응하는 다수 의 단백질이 Fe-클러스터 합성에도 관여하는 것으로 밝혀져 있고 이들 단백질은 nifS 클래스 단백질의 구성원이다. 리포산은 옥타노산으로부터 유도되고, 에너지 대사에서 보조효소로 작용하는데, 상기 리포산은 에너지 대사에서 피루베이트 디히드로게나제 (dehydrogenase) 결합체 및 α-케토글루타레이트 디히드로게나제 결합체의 일부가 된다. 폴레이트는 모두 엽산의 유도체 물질의 군이고, 엽산은 L-글루탐산, p-아미노벤조산 및 6-메틸프테린으로부터 유도된다. 대사 중간체인 구아노신-5'-트리포스페이트 (GTP), L-글루탐산 및 p-아미노벤조산으로부터 출발하는, 엽산 및 그의 유도체의 생합성은 일부 미생물에서 상세히 연구되어 있다.

코리노이드 (예를 들어, 코발라민 및 특히 비타민 B₁₂) 및 포피린은 테트라피롤 고리계에 의해 특징지워지는 화학물질의 군에 속한다. 비타민 B₁₂의 생합성은 그 특징이 완전히 규명되어 있지는 않지만 다수의 효소 및 기질이 현재 공지되어 있다는 점에서 충분히 복잡하다. 니코틴산 (니코티네이트) 및 니코틴아미드는 "니아신"으로도 지칭되는 피리딘 유도체이다. 니아신은 중요한 보조효소인 NAD (니코틴아미드 아데닌 디뉴클레오티드), NADP (니코틴아미드 아데닌 디뉴클레오티드 포스페이트) 및 그들의 환원형의 전구체이다.

이들 화합물의 대량 생산은 비록 이들 화학물질 중 일부, 예를 들어, 리보플라빈, 비타민 B₆, 판토테네이트 및 비오틴이 미생물의 대량 배양에 의해서도 생산된다 하더라도 세포-유리 화학 합성에 주로 의존한다. 비타민 B₁₂만이 발효에 의해 전적으로 생성되는데, 이는 비타민 B₁₂의 합성의 복잡성 때문이다. 시험관내 방법론은 재료 및 시간, 종종 많은 비용의 상당한 투입을 요구한다.

C. 퓨린, 피리미딘, 뉴클레오시드 및 뉴클레오티드의 대사 및 용도

퓨린 및 피리미딘 대사 유전자 및 이들의 상응하는 단백질은 종양 질환 및 바이러스 감염의 치료에 중요한 표적이다. 용어 "퓨린" 또는 "피리미딘"은 핵산, 보조효소 및 뉴클레오티드의 구성 성분인 질소 함유 염기를 포함한다. 용어 "뉴클레오티드"는 질소 함유 염기, 오탄당 (RNA의 경우, 상기 당은 리보스이고, DNA의 경우, 상기 당은 D-데옥시리보스임) 및 인산으로 구성된, 핵산 분자의 기본 구조 단위를 포함한다. 용어 "뉴클레오시드"는 뉴클레오티드의 전구체로 작용하지만 뉴클레오티드가 갖는 인산 잔기가 없는 분자를 포함한다. 이들 분자의 생합성을 억제하거나 핵산 분자를 형성하기 위한 이들 분자의 동원(動員)을 억제함으로써, RNA 및 DNA 합성 억제가 가능하고, 암세포에 표적화시키는 방식으로 상기 활성을 억제하여 종양 세포의 분열 및 복제 능력을 억제할 수 있다.

또한, 핵산 분자를 형성하기 보다는 에너지 저장물 (즉, AMP) 또는 보조효소 (즉, FAD 및 NAD)로 작용하는 뉴클레오티드도 있다.

이들 화학물질이 퓨린 및(또는) 피리미딘 대사에 영향을 줌으로써 상기 의학적 증상에 사용될 수 있다는 것은 여러 가지 문헌에 기재되어 있다 (예를 들어, 문헌 [Christopherson, R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med. Res. Reviews 10: 505-548]). 퓨린 및 피리미딘 대사에 관여하는 효소의 연구는 예를 들어, 면역억제제 또는 항증식제로 사용할 수 있는 신약의 개발에 초점을 두고 있다 (문헌 [Smith, J.L., "Enzymes in nucleotide synthesis." Curr. Opin. Struct. Biol. 5 (1995) 752-757; Biochem Soc. Transact. 23: 877-902]). 그러나, 퓨린 및 피리미딘 염기, 뉴클레오시드 및 뉴클레오티드는 여러 가지 정밀 화학물질 (예를 들어, 티아민, S-아데노실-메티오닌, 폴레이트 또는 리보플라빈)의 생합성에 있어서 중간체로서의 용도, 세포를 위한 에너지 전달자 (예를 들어, ATP 또는 GTP)로서의 용도, 및 통상적으로 향 상승제 (예를 들어, IMP 또는 GMP)로 사용되는 화학물질 자체를 위한 용도 또는 여러 가지 의학 용도를 갖는다 (예를 들어, 문헌 [Kuninaka, A. (1996) "Nucleotides and Related Compounds in Biotechnology" Vol. 6, Rehm et al., eds. VCH: Weinheim, p.561-612] 참조). 또한, 퓨린, 피리미딘, 뉴클레오시드 또는 뉴클레오티드 대사에 관여하는 효소를 표적으로 사용하여 살진균제, 제초제 및 살충제를 비롯한, 농작물 보호용 화학물질을 개발하는 연구가 증가하고 있다.

박테리아에서 이들 화합물 대사의 특징은 규명되어 있다 (자세한 내용은 예를 들어, 문헌 [Zalkin, H. and Dixon, J. E. (1992) "De novo purine nucleotide biosynthesis", in Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p.259-287], 및 문헌 [Michal, G. (1999) "Nucleotides and Nucleosides"; Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley, New York] 참조). 퓨린의 대사는 집 중적인 연구의 대상이고, 세포의 정상적인 기능에 필수적이다. 고등 동물의 손상된 퓨린 대사는 통풍과 같은 심각한 질환을 초래할 수 있다. 퓨린 뉴클레오티드는 구아노신-5'-모노포스페이트 (GMP) 또는 아데노신-5'-모노포스페이트 (AMP)를 생성시키는 중간체 화합물인 이노신-5'-포스페이트 (IMP)를 통해 일련의 단계에서 리보스-5-포스페이트로부터 합성되고, 이들 GMP 또는 AMP로부터 뉴클레오티드로 사용되는 트리포스페이트가 용이하게 형성된다. 이들 화합물은 에너지 저장물로도 사용되므로, 이들의 분해는 세포에서 다수의 다양한 생합성 과정을 위한 에너지를 공급한다. 피리미딘 생합성은 리보스-5-포스페이트로부터의 유리딘-5'-모노포스페이트 (UMP)의 형성에 의해 진행된다. 그 다음, UMP가 시티딘-5'-트리포스페이트 (CTP)로 전환된다. 이들 모든 뉴클레오티드의 디옥시 형태는 뉴클레오티드의 디포스페이트 리보스 형태가 뉴클레오티드의 디포스페이트 데옥시리보스 형태로 전환되는 1 단계 환원 반응에서 생성된다. 인산화 후에 이들 분자는 DNA 합성에 참여할 수 있다.

D. 트레할로스의 대사 및 용도

트레할로스는 α,α-1,1 결합에 의해 결합된 두 가지 당 분자로 구성된다. 트레할로스는 감미료, 건조 또는 동결 식품용 첨가제로서 식품 산업, 및 음료 산업에 통상적으로 사용된다. 그러나, 제약, 화장품 및 생물공학 산업에도 사용된다 (예를 들어, 문헌 [Nishimoto et al., (1998) 미국 특허 제5,759,610호], 문헌 [Singer, M.A. and Lindquist, S. Trends Biotech. 16 (1998) 460-467], 문헌 [Paiva, C.L.A. and Panek, A.D. Biotech. Ann. Rev. 2 (1996) 293-314], 문헌 [Shiosaka, M. J. Japan 172 (1997) 97-102] 참조). 트레할로스는 다수의 미생물로부터 효소에 의해 생성되고 주변 배지내로 자연적으로 방출되는데, 트레할로스는 당업계에 공지된 방법을 이용하여 상기 배지로부터 수득할 수 있다.

II. 대사 조절의 메카니즘

모든 살아있는 세포는 많은 대사 경로가 서로 연결되어 있는 복잡한 이화 및 동화 능력을 갖는다. 이러한 극히 복잡한 대사 네트워크의 각종 부분들 사이에 평형을 유지하기 위하여, 세포는 정밀하게 조율된 조절 네트워크를 이용한다. 효소 합성 및 효소 활성을 독립적으로 또는 동시에 조절함으로써, 세포는 완전하게 상이한 대사 경로의 활성을 조절하여 세포의 변화하는 요구들을 충족시킬 수 있다.

효소 합성의 유도 또는 억제는 전사 또는 번역 수준 중 하나에서 또는 전사와 번역 수준 모두에서 발생할 수 있다 (자세한 것은 문헌 [Lewin, B. (1990) Genes IV, Part 3: "Controlling prokaryote genes by transcription" Oxford University Press, Oxford, pp. 213-301]과 그의 참고문헌, 및 문헌 [Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley & Sons] 참조). 상기 공지된 조절 방법 모두는 각종 외적 효과 (예컨대 온도, 영양소 공급 또는 빛)에 스스로 반응하는 추가의 유전자에 의해 매개된다. 상기 유형의 조절에 관여하는 단백질 인자의 예는 전사 인자를 포함한다. 이들은 DNA와 결합하여 유전자의 발현을 증가시키거나 (E. coli ara 오페론의 경우 양의 조절) 감소시키는 (E. coli lac 오페론의 경우 음의 조절) 단백질이다. 이들 발현-조정 전사 인자는 스스로 조절될 수 있다. 그의 활성은 예를 들어 DNA-결합 단백질에 결합된 저분자량 화합물에 의해 조절될 수 있는데, 상기 단백질과 DNA상의 적절한 결합 부위와의 결합이 자극되거나 (ara 오페론에 대해서는 아라비노스의 경우와 같음) 억제됨으로써 (lac 오페론에 대해서는 락토스의 경우와 같음) 조절된다 (예를 들어, 문헌 [Helmann, J.D. and Chamberlin, M.J. (1988) "Structure and function of bacterial sigma factors" Ann. Rev. Biochem. 57: 839-872]; [Adhya, S. (1995) "The lac and gal operons today" and Boos, W. et al., "The maltose system" both in Regulation of Gene Expression in Escherichia coli (Lin, E.C.C. and Lynch, A.S. editors) Chapman & Hall: New York, pp. 181-200 and 201-229]; 및 [Moran, C.P. (1993) "DNA polymerase and transcription factors" in: Bacillus subtilis and other Gram-positive bacteria, Sonenshein, A.L. editors ASM: Washington D.C. pp. 653-667] 참조).

단백질 합성은 전사 수준에서 뿐 아니라 번역 수준에서도 조절된다. 상기 조절은 리보솜이 하나 이상의 mRNA와 결합하는 능력, 리보솜과 mRNA의 결합, mRNA 2차 구조의 유지 또는 제거, 특정 유전자에 대한 통상적 또는 덜 통상적인 코돈의 이용, 하나 이상의 tRNA의 풍부도를 변형시키는 것을 포함하는 많은 메카니즘 및 약독화와 같은 특이적 조절 메카니즘을 통해 수행될 수 있다 (문헌 [Vellanoweth, R.I. (1993) Translation and its regulation in Bacillus subtilis and other Gram-positive bacteria, Sonenshein, A.L. et al. editors ASM: Washington, D.C., pp. 699-711] 및 그의 참고문헌 참조).

전사 및 번역의 조절은 단일 단백질을 향해 지시되거나 (순차적 조절), 동시 에 각종 대사 경로의 복수개의 단백질을 향해 지시될 수 있다 (통합적 조절). 발현이 통합적 방식으로 조절되는 유전자는 종종 게놈상에서 오페론 또는 레귤론에 근접하게 위치한다. 유전자 전사 및 유전자 번역의 이러한 상향 또는 하향 조절은 기질 (하나 이상의 대사 경로에 사용되는 전구체 및 중간체), 이화생성물 (당과 같은 복합체 유기 분자의 분해로 인한 에너지 생산과 연결된 생화학적 경로에 의해 생산된 분자) 및 최종 생성물 (대사 경로의 마지막에 수득되는 분자) 등과 같은 각종 인자의 세포내 또는 세포외 양에 의해 제어된다. 특정 대사 경로의 활성에 요구되는 효소를 코딩하는 유전자의 발현은 상기 대사 경로에 대한 다량의 기질 분자에 대해 유도된다. 상응하게, 상기 유전자 발현은 상기 경로의 최종 생성물이 세포간에 다량 존재함으로써 억제된다 (Snyder, L. and Champness, W. (1977) The Molecular Biology of Bacteria ASM: Washington). 유전자 발현은 환경적 조건 (예컨대 열, 산화적 스트레스 또는 기아)과 같은 내부 및 외부 인자에 의해 조절될 수도 있다. 이들 전체적인 환경적 변화는 DNA에 결합함으로써 유전자 발현을 직접적으로 또는 간접적으로 (추가의 유전자 또는 단백질을 통해) 유도하는 구체적인 조정 유전자의 발현을 변화시키고, 그로 인해 전사를 유도하거나 억제한다 (예를 들어, 문헌 [Lin, E.C.C. and Lynch, A.S. editors (1995) Regulation of Gene Expression in Escherichia coli, Chapman & Hall: New York] 참조).

세포내 대사를 조절할 수 있는 또다른 메카니즘은 단백질 수준에서 발생한다. 상기 조절은 단백질의 정상 기능을 방해하거나 허용하는 저분자량 성분의 결합 또는 다른 효소의 활성을 통해 수행된다. 저분자량 화합물의 결합에 의한 단백 질 조절의 예는 GTP 또는 NAD의 결합을 포함한다. 저분자량 화학물질의 결합은 예를 들어 GTP-결합 단백질의 경우 보통 가역적이다. 이들 단백질은 (GTP 또는 GDP와 결합하여) 2가지 상태로 존재하는데, 한 상태는 단백질의 활성형이고 다른 상태는 비활성형이다.

단백질 활성은 보통 단백질의 공유결합적 변형 (즉, 히스티딘 또는 아스파르테이트와 같은 아미노산 잔기의 인산화 또는 메틸화)을 통한 다른 효소의 작용으로 조절된다. 상기 공유결합적 변형은 보통 가역적이고, 이는 반대 활성을 가진 효소에 의해 수행된다. 이의 예는 단백질 인산화에서의 키나제 및 포스포릴라제의 반대 활성이다: 단백질 키나제는 표적 단백질 (예컨대 세린 또는 트레오닌)상의 특이적 잔기를 인산화하는 반면, 단백질 포스포릴라제는 상기 단백질로부터 포스페이트 기를 제거한다. 다른 단백질의 활성을 조정하는 효소는 보통 외부 자극에 의해 스스로 조정된다. 이들 자극은 감각기로서 작용하는 단백질에 의해 매개된다. 이들 감각기 단백질이 상기 외적 신호를 매개하는 널리 공지된 메카니즘은 이량체화이나, 다른 메카니즘도 공지되어 있다 (예를 들어, 문헌 [Msadek, T. et al. (1993) "Two-component Regulatory Systems" in: Bacillus subtilis and Other Gram-Positive Bacteria, Sonenshein, A.L. et al., editors, ASM: Washington, pp. 729-745] 및 그의 참고문헌).

미생물에서 세포내 대사를 제어하는 조절 네트워크의 상세한 이해는 발효에 의해 고수율로 화학물질을 생산하기 위해서 중대하다. 대사 경로를 하향조절하기 위한 제어 시스템은 관심 화학물질의 합성을 개선시키기 위해 제거되거나 감소될 수 있고, 이와 상응하게 관심 생성물의 대사 경로를 상향조절하기 위한 제어 시스템은 활성 면에서 구조적으로 활성화되거나 최적화될 수 있다 (문헌 [Hirose, Y. and Okada, H. (1979) "Microbial Production of Amino Acids" in: Peppler, H.J. and Perlman, D. (editors) Microbial Technology 2nd edition, Vol. 1, Chapter 7, Academic Press, New York] 참조).

III. 본 발명의 요소 및 방법

본 발명은, 적어도 부분적으로는, 코리네박테리움 글루타미쿰에서의 하나 이상의 대사 경로를 전사, 번역 또는 후번역 방법에 의해 조절하는, 본원에서 MP 핵산 및 MP 단백질 분자로 언급되는 새로운 분자의 검출을 기초로 한다. 한 실시양태에서, MP 분자는 코리네박테리움 글루타미쿰에서의 대사 경로를 전사, 번역 또는 후번역적으로 조절한다. 바람직한 실시양태에서, 코리네박테리움 글루타미쿰에서의 하나 이상의 대사 경로를 조절하기 위한 본 발명의 MP 분자의 활성은 이 유기체에 의한 관심 정밀 화학물질의 생산에 영향을 준다. 특히 바람직한 실시양태에서, 본 발명의 MP 단백질이 조절하는 코리네박테리움 글루타미쿰 대사 경로가 생산성 또는 효율 면에서 조정되도록 본 발명의 MP 분자의 활성을 조정하여, 코리네박테리움 글루타미쿰에 의한 관심 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율을 직접적 또는 간접적으로 조절한다.

용어 "MP 단백질" 또는 "MP 폴리펩티드"에는 코리네박테리움 글루타미쿰에서의 대사 경로를 전사, 번역 또는 후번역적으로 조절하는 단백질이 포함된다. MP 단백질의 예에는 표 1 및 첨부문서 A에 기재된 MP 유전자에 의해 코딩되는 것들이 포함된다. 용어 "MP 유전자" 또는 "MP 핵산 서열"에는 코딩 영역 및 이에 상응하는 비번역 5' 및 3' 서열 영역으로 구성된, MP 단백질을 코딩하는 핵산 서열이 포함된다. MP 유전자의 예는 표 1에 기재된다. 용어 "생산량" 및 "생산성"은 당업계에 공지되어 있으며 소정의 기간 및 소정의 발효 부피에서 생산될 발효 생성물 (예컨대 관심 정밀 화학물질)의 농도 (예컨대 1 L 당 생성물 질량 (kg)/시간)를 포함한다. 용어 "생산 효율"은 특정 생산량 (예를 들어, 세포에 의한 정밀 화학물질의 처리량이 특정 속도에 도달하는데 필요한 시간) 달성에 필요한 시간을 포함한다. 용어 "수율" 또는 "생성물/탄소 수율"은 당업계에 공지되어 있으며 탄소 공급원을 생성물 (즉, 정밀 화학물질)로 전환시키는 효율을 포함한다. 즉, 이는 예를 들어 통상적으로 탄소 공급원 1 kg 당 생성물의 질량 (kg)으로 표현된다. 화합물의 수율 또는 생산 증가는 특정량의 배양물에서 소정의 시간에 걸쳐 수득되는 상기 분자의 양 또는 이 화합물의 적합하게 수득되는 분자의 양을 증가시킨다. 용어 "생합성" 및 "생합성 경로"는 당업계에 공지되어 있으며 세포가 중간체로부터 화합물, 바람직하게는 유기 화합물을 예를 들어 다단계 과정 또는 고도로 조절되는 과정을 통해 합성하는 것을 포함한다. 용어 "분해" 및 "분해 경로"는 당업계에 공지되어 있으며 세포가 화합물, 바람직하게는 유기 화합물을 예를 들어 다단계 과정 또는 고도로 조절되는 과정을 통해 분해 생성물 (더욱 일반적인 용어로는, 더 작거나 덜 복잡한 분자)로 절단하는 것을 포함한다. 용어 "대사"는 당업계에 공지되어 있으며 유기체에서 일어나는 전반적인 생화학적 반응을 포함한다. 이때, 특정 화합물의 대사 (예컨대 글리신 등과 같은 아미노산의 대사)는 그 화합물의 세포내 모 든 생합성, 변형 및 분해 경로를 포함한다. 용어 "조절"은 당업계에 공지되어 있으며, 또다른 단백질의 활성을 제어하기 위한 단백질의 활성을 포함한다. 용어 "전사 조절"은 당업계에 공지되어 있으며 표적 단백질을 코딩하는 DNA의 mRNA로의 전환을 억제하거나 활성화시키는 단백질의 활성을 포함한다. 용어 "번역 조절"은 당업계에 공지되어 있으며 표적 단백질을 코딩하는 mRNA의 단백질 분자로의 전환을 억제하거나 활성화시키는 단백질의 활성을 포함한다. 용어 "후번역 조절"은 당업계에 공지되어 있으며 표적 단백질을 공유결합적으로 (예컨대 메틸화, 글리코실화 또는 인산화에 의해) 변형시킴으로써 표적 단백질의 활성을 억제하거나 개선시키는 단백질의 활성을 포함한다.

또다른 실시양태에서, 본 발명의 MP 분자는 미생물, 예를 들어 코리네박테리움 글루타미쿰 내에서 관심 분자, 예를 들어 정밀 화학물질의 생산을 조정할 수 있다. 유전자 재조합 기술을 이용하여 본 발명의 1종 이상의 본 발명의 조절 단백질을 조작함으로써 이들의 기능을 조정할 수 있다. 예를 들어, 생합성 효소를 효율 면에서 개선시키거나 그의 알로스테릭 제어 영역을 파괴하여 화합물 생산의 피드백 억제를 방해하는 것이 가능하다. 따라서, 분해 효소는 세포 생존력을 손상시키지 않으면서 관심 화합물에 대한 그의 분해 활성을 감소시키도록 치환, 결실 또는 부가에 의해 삭제 또는 변형될 수 있다. 어떤 경우에는, 임의의 상기 관심 정밀 화학물질의 전반적 효율 또는 생산 속도를 증가시키는 것이 가능하다.

또한, 본 발명의 단백질 및 뉴클레오티드 분자에서의 이러한 변형은 정밀 화학물질의 생산을 간접적으로 개선시킬 수도 잇다. 세포내 대사 경로의 조절 메카 니즘은 반드시 연결되어 있고, 한 대사 경로의 활성화는 그에 수반되는 방식으로 또다른 대사 경로를 억제 또는 활성화시킬 수 있다. 본 발명의 1종 이상의 단백질의 활성을 조정하는 것은 다른 정밀 화학물질 생합성의 생산 또는 활성 효율 또는 분해 경로에 영향을 줄 수 있다. 이들 대사 경로는 서로 연결되어 있으므로 아미노산 생합성에서 특정 단백질을 코딩하는 유전자의 전사를 억제하는 MP 단백질의 능력을 감소시킴으로써 동시에 다른 아미노산 생합성 경로를 억제할 수 있게 된다. 본 발명의 MP 단백질을 변형시킴으로써 그의 세포외 환경으로부터 일정한 수준의 세포 성장 및 세포 분열로 분리하는 것이 가능하고; 성장 및 세포 분열을 위한 세포외 조건이 최적에 이르지 못해 이제 상기 기능이 결핍된 경우, 보통 뉴클레오티드의 생합성을 억제하는 MP 단백질에 영향을 줌으로써 세포외 조건이 불량할지라도 성장할 수 있게 하는 것이 가능하다. 상기 인자에 대한 세포내 조절 시스템이 제거된 후 온도, 영양소 공급 또는 공기공급에 대한 배양물 조건이 종종 최적에 이르지 못하지만 성장 및 세포 분열을 여전히 촉진시킬 수 있는 대규모 발효 배양의 경우 이는 특히 중요하다.

본 발명 핵산 서열 제조에 적합한 출발점은 아메리칸 타입 컬쳐 콜렉션 (American Type Culture Collection)에서 ATCC 13032로 입수할 수 있는 코리네박테리움 글루타미쿰 균주의 게놈이다.

본 발명의 핵산 서열은 표 1에 나타낸 변형을 통해 통상의 방법을 이용하여 이들 핵산 서열로부터 제조할 수 있다.

본 발명의 MP 단백질 또는 그의 생물학적 활성 절편 또는 단편은 코리네박테 리움 글루타미쿰에서의 대사 경로를 전사, 번역 또는 후번역적으로 조절할 수 있거나, 표 1에 나열한 1개 이상의 활성을 가질 수 있다.

하기 단락에서는 본 발명의 여러가지 측면을 더욱 상세하게 기재한다:

A. 단리된 핵산 분자

본 발명의 한 측면은 MP 폴리펩티드를 코딩하는 단리된 핵산 분자 또는 그의 생물학적 활성 절편에 관한 것이며, MP-코딩 핵산 (예컨대 MP DNA)의 확인 또는 증폭을 위한 혼성화 프로브 또는 프라이머로 사용하기에 충분한 핵산 단편에 관한 것이다. 본원에서 사용된 바와 같이, 용어 "핵산 분자"는 DNA 분자 (예컨대 cDNA 또는 게놈 DNA) 및 RNA 분자 (예컨대 mRNA)를 포함하며, 또한 뉴클레오티드 유사체에 의해 생성된 DNA 또는 RNA 유사체를 포함한다. 추가로, 상기 용어는 코딩 유전자 영역의 3' 및 5' 말단에 위치한 비번역 서열 (코딩 영역의 5' 말단 상류 서열의 뉴클레오티드 약 100개 이상 및 코딩 유전자 영역의 3' 말단 하류 서열의 뉴클레오티드 약 20개 이상)을 포함한다. 핵산 분자는 단일 가닥일 수도 있고 이중 가닥일 수도 있으나, 바람직하게는 이중 가닥 DNA이다. "단리된" 핵산 분자는 해당 핵산의 천연 공급원에 존재하는 다른 핵산 분자가 제거된 것이다. "단리된" 핵산은 해당 핵산이 기원된 유기체의 게놈 DNA의 핵산을 천연적으로 플랭킹 (flanking)하는 임의의 서열 (예를 들어 해당 핵산의 5' 또는 3' 말단에 위치한 서열)을 보유하지 않는 것이 바람직하다. 여러 실시양태에서, 단리된 MP 핵산 분자는 해당 핵산이 기원된 세포 (예컨대 코리네박테리움 글루타미쿰 세포)의 게놈 DNA의 핵산 분자를 천연적으로 플랭킹하는 뉴클레오티드 서열을 예를 들어 약 5 kb 미만, 약 4 kb 미 만, 약 3 kb 미만, 약 2 kb 미만, 약 1 kb 미만, 약 0.5 kb 미만 또는 약 0.1 kb 미만으로 보유할 수 있다. 게다가, cDNA 분자 등과 같은 "단리된" 핵산 분자에는, 재조합 기술로 제조된 경우에는 다른 세포내 물질 또는 배양 배지가 본질적으로 존재하지 않으며 화학적으로 합성된 경우에는 화학적 전구체 또는 다른 화학물질이 본질적으로 존재하지 않을 수 있다.

본 발명의 핵산 분자, 예를 들어 첨부문서 A의 뉴클레오티드 서열 또는 그의 절편을 보유하는 핵산 분자는 분자생물학 표준 기술 및 본원에 제공된 서열 정보를 통해 단리할 수 있다. 예를 들면, 코리네박테리움 글루타미쿰 MP cDNA는 첨부문서 A로부터의 완전 서열 또는 그의 절편을 혼성화 프로브로서 이용하고 표준 혼성화 기술 (예를 들어 문헌 [Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989]에 기재된 바와 같음)을 이용하여 코리네박테리움 글루타미쿰 뱅크 (bank)에서 단리할 수 있다. 추가로, 첨부문서 A로부터의 완전 서열 또는 그의 절편을 포함하는 핵산 분자는 상기 서열을 기초로 생성한 올리고뉴클레오티드 프라이머를 사용하는 폴리머라제 연쇄 반응으로 단리할 수 있다 (예를 들어, 첨부문서 A로부터의 완전 서열 또는 그의 절편을 포함하는 핵산 분자는 첨부문서 A로부터의 동일 서열을 기초로 생성한 올리고뉴클레오티드 프라이머를 사용한 폴리머라제 연쇄 반응으로 단리할 수 있음). 예를 들면, mRNA는 정상적인 내피 세포에서 단리할 수 있고 (예를 들어 문헌 [Chirgwin et al. (1979) Biochemistry 18:5294-5299]의 구아니디늄-티오시아네이 트 추출 방법에 의한 단리), cDNA는 역전사효소 (예를 들어 미국 메릴랜드주 베테스다에 소재하는 Gibco/BRL이 시판하는 몰로니 (Moloney)-MLV 역전사효소 또는 미국 플로리다주 세인트 페터스부르크에 소재하는 세이카가꾸 아메리카, 인크. (Seikagaku America, Inc.)에서 구입한 AMV 역전사효소)로 제조할 수 있다. 폴리머라제 연쇄 반응을 통한 증폭에 사용하기 위한 합성 올리고뉴클레오티드 프라이머는 첨부문서 A에 나타낸 임의의 뉴클레오티드 서열을 기초로 생성할 수 있다. 본 발명의 핵산은 cDNA로 증폭시킬 수도 있고, 별법으로는 주형으로서의 게놈 DNA 및 PCR 표준 증폭 기술에 따른 적합한 올리고뉴클레오티드 프라이머를 사용하여 증폭시킬 수도 있다. 이러한 방법으로 증폭된 핵산을 적합한 벡터에 클로닝하고 DNA 서열 분석함으로써 특성화할 수 있다. 또한, MP 뉴클레오티드 서열에 상응하는 올리고뉴클레오티드는 자동 DNA 합성기 등과 같은 표준 합성법을 통해 제조할 수도 있다.

바람직한 실시양태에서, 본 발명의 단리된 핵산 분자는 첨부문서 A에 기재한 임의의 뉴클레오티드 서열을 포함한다.

추가의 바람직한 실시양태에서, 본 발명의 단리된 핵산 분자는 첨부문서 A에 나타낸 임의의 뉴클레오티드 서열 또는 그의 절편과 상보적인 핵산 분자를 포함하며, 상기 핵산 분자는 첨부문서 A에 나타낸 임의의 서열과 혼성화하여 안정한 이중나선을 형성할 정도로 첨부문서 A에 나타낸 임의의 뉴클레오티드 서열과 충분히 상보적이다.

한 실시양태에서, 본 발명의 핵산 분자는 여전히 코리네박테리움 글루타미쿰 에서의 대사 경로를 전사, 번역 또는 후번역적으로 조절할 수 있는 능력을 보유하는, 첨부문서 B의 아미노산 서열에 충분한 상동성이 있는 아미노산 서열을 포함하는 단백질 또는 그의 절편을 코딩한다. 본원에서 사용된 바와 같이, 용어 "충분한 상동성이 있는"은 코리네박테리움 글루타미쿰에서 대사 경로를 전사, 번역 또는 후번역적으로 조절할 수 있는, 아미노산 서열이 첨부문서 B의 아미노산 서열과 동일한 아미노산 잔기 또는 그와 동등한 아미노산 잔기 (예를 들어 첨부문서 B에 나타낸 임의의 서열의 아미노산 잔기의 측쇄와 유사한 측쇄를 갖는 아미노산 잔기)를 최소수로 보유하는 단백질 또는 그의 절편과 관련된 것이다. 본원에 기재한 바와 같이, 이들 대사 경로의 단백질 성분은 1종 이상의 정밀 화학물질의 생합성 또는 분해를 조절할 수 있다. 이러한 활성의 예를 또한 본원에 기재한다. 따라서, "MP 단백질의 기능"은 1종 이상의 정밀 화학물질의 대사 경로에 대한 전반적 조절에 관한 것이다. 표 1은 MP 단백질 활성의 예를 나열한다.

본 발명의 MP 핵산 분자가 코딩하는 단백질의 절편은 임의의 MP 단백질의 생물학적 활성 절편인 것이 바람직하다. 본원에서 사용된 바와 같이, 용어 "MP 단백질의 생물학적 활성 절편"은 코리네박테리움 글루타미쿰에서 대사 경로를 전사, 번역 또는 후번역적으로 조절할 수 있는 MP 단백질의 절편, 예를 들어 도메인 또는 모티프를 포함하는 것으로 의도되며, 표 1에 나타낸 활성을 갖는다. MP 단백질 또는 그의 생물학적 활성 절편이 코리네박테리움 글루타미쿰에서 대사 경로를 전사, 번역 또는 후번역적으로 조절할 수 있는 지의 여부는 효소 활성 분석을 수행하여 결정할 수 있다. 이러한 분석 방법은 실시예 단락의 실시예 8에 상세하게 기재한 바와 같이 당업자에게 공지되어 있다.

또한, 당업자는 집단 내에 존재할 수 있는 천연 MP-서열 변이체 뿐 아니라 첨부문서 A의 뉴클레오티드 서열에 돌연변이에 의한 변화를 도입시켜, 코딩된 MP 단백질의 아미노산 서열을 변화시키면서도 MP 단백질의 기능을 손상시키지 않을 수 있음을 이해할 것이다. 따라서, 예를 들어 첨부문서 A의 서열에서 "비필수" 아미노산 잔기에서의 아미노산 치환을 일으키는 뉴클레오티드 치환을 생성할 수 있다. "비필수" 아미노산 잔기는 임의의 MP 단백질의 야생형 서열 (첨부문서 B)을 변경시키면서도 상기 MP 단백질의 활성을 변화시키지 않을 수 있는 잔기이며, "필수" 아미노산 잔기는 MP 단백질 활성에 필요한 잔기이다. 그러나, 다른 아미노산 잔기 (예컨대 MP 활성을 갖는 도메인에서 비-보존 또는 단지 반-보존된 아미노산 잔기)가 활성에 비필수일 수도 있기 때문에 이를 변형시키면서 MP 활성은 변형시키지 않을 수도 있을 것이다.

첨부문서 B의 단백질 서열에 상동성이 있는 MP 단백질을 코딩하는 단리된 핵산 분자는 첨부문서 A의 뉴클레오티드 서열에 하나 이상의 뉴클레오티드 치환, 부가 또는 결실을 도입하여 생성할 수 있으며, 이에 의해 코딩된 단백질에는 하나 이상의 아미노산 치환, 부가 또는 결실이 도입된다. 상기 돌연변이는 부위-지정 돌연변이유발법 및 PCR-매개 돌연변이유발법 등과 같은 표준 기술을 통해 첨부문서 A에 나타낸 임의의 서열에 도입될 수 있다. 비필수라고 예측되는 하나 이상의 아미노산 잔기에 보존적 아미노산 치환을 도입하는 것이 바람직하다. "보존적 아미노산 치환"은 해당 아미노산 잔기를 유사한 측쇄를 갖는 아미노산 잔기로 대체한다. 유사한 측쇄를 갖는 아미노산 잔기의 군은 당업계에 규정되어 있다. 이들 군은 염기성 측쇄를 갖는 아미노산 (예컨대 리신, 아르기닌, 히스티딘), 산성 측쇄를 갖는 아미노산 (예컨대 아스파르트산, 글루탐산), 대전되지 않은 극성 측쇄를 갖는 아미노산 (예컨대 글리신, 아스파라긴, 글루타민, 세린, 트레오닌, 티로신, 시스테인), 비극성 측쇄를 갖는 아미노산 (예컨대 알라닌, 발린, 루이신, 이소루이신, 프롤린, 페닐알라닌, 메티오닌, 트립토판), 베타-분지된 측쇄를 갖는 아미노산 (예컨대 트레오닌, 발린, 이소루이신) 및 방향족 측쇄를 갖는 아미노산 (예컨대 티로신, 페닐알라닌, 트립토판, 히스티딘)을 포함한다. 따라서, MP 단백질에서 비필수라고 예측되는 아미노산 잔기는 동일 측쇄군의 또다른 아미노산 잔기로 대체되는 것이 바람직하다. 별법으로, 또다른 실시양태에서는 상기 돌연변이를 MP-코딩 서열 전체 또는 일부에 예를 들어 포화 돌연변이유발법 등을 통해 무작위로 도입할 수 있고, 생성되는 돌연변이체를 본원에 기재한 MP 활성에 대해 시험하여 MP 활성을 유지하는 돌연변이체를 확인할 수 있다. 첨부문서 A에 나타낸 임의의 서열을 돌연변이유발시킨 후에, 코딩되는 단백질을 재조합으로 발현시킬 수 있고, 상기 단백질의 활성을 예를 들어 본원에 기재한 분석법을 이용하여 측정할 수 있다 (실시예 단락의 실시예 8 참조).

B. 재조합 발현 벡터 및 숙주 세포

본 발명의 또다른 측면은 MP 단백질 (또는 그의 절편)을 코딩하는 핵산을 함유하는 벡터, 바람직하게는 발현 벡터에 관한 것이다. 본원에서 사용된 바와 같이, 용어 "벡터"는 그와 결합된 또다른 핵산을 수송할 수 있는 핵산 분자와 관련된 것이다. 벡터의 유형 중 하나는 추가의 DNA 세그먼트가 라이게이션될 수 있는 원형 이중 가닥 DNA 루프를 의미하는 용어인 "플라스미드"이다. 또다른 유형의 벡터는 바이러스 벡터이며, 여기서는 추가의 DNA 세그먼트를 바이러스 게놈에 라이게이션시킬 수 있다. 몇몇 벡터들은 그들이 도입된 숙주 세포 내에서 자율적으로 복제될 수 있다 (예를 들어, 박테리아 복제 기점을 갖는 박테리아 벡터 및 에피솜 포유동물 벡터). 다른 벡터들 (예컨대 비-에피솜 포유동물 벡터)은 숙주 세포에 도입될 때 상기 숙주 세포의 게놈에 통합됨으로써 숙주 게놈과 함게 복제된다. 추가로, 몇몇 벡터들은 기능적으로 연결된 유전자의 발현을 제어할 수 있다. 본원에서는 이러한 벡터를 "발현 벡터"라고 지칭한다. 통상, DNA 재조합 기술에 사용되는 발현 벡터는 플라스미드 형태이다. 플라스미드가 가장 일반적으로 사용되는 유형의 벡터이므로, 본 명세서에서는 "플라스미드" 및 "벡터"를 혼용하여 사용한다. 본 발명은 유사한 기능을 발휘하는 상기 다른 유형의 발현 벡터, 예를 들어 바이러스 벡터 (예컨대 복제-결핍 레트로바이러스, 아데노바이러스 및 아데노바이러스-관련 바이러스)를 포함한다.

본 발명의 재조합 발현 벡터는 본 발명의 핵산을 숙주 세포에서의 발현에 적합한 형태로 포함하며, 이는 재조합 발현 벡터가 발현에 사용될 숙주 세포를 기초로 하여 선택되고 발현될 핵산 서열에 기능적으로 연결된 하나 이상의 조절 서열을 포함함을 의미한다. 재조합 발현 벡터에서, 용어 "기능적으로 연결된"은 관심 뉴클레오티드 서열이 그의 발현 (예를 들어 시험관내 전사/번역 시스템에서 발현되거나 또는 상기 벡터가 숙주 세포에 도입된 경우에는 그 숙주 세포에서 발현됨)이 가 능하도록 조절 서열(들)에 결합된 것을 의미한다. 용어 "조절 서열"은 프로모터, 인핸서 및 다른 발현 제어 요소 (예컨대 폴리아데닐화 신호)를 포함하는 것으로 의도된다. 이러한 조절 서열은 예를 들어 문헌 [Goeddel: Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990)] 등에 기재되어 있다. 조절 서열은 여러 유형의 숙주 세포에서 뉴클레오티드 서열의 구성적 (constitutive) 발현을 제어하는 서열 및 특정 숙주 세포에서만 뉴클레오티드 서열의 발현을 제어하는 서열을 포함한다. 당업자라면, 발현 벡터의 고안이 형질전환시킬 숙주 세포의 선택, 관심 단백질 발현 정도 등과 같은 인자에 따라 달라질 수 있음을 이해할 것이다. 본 발명의 발현 벡터를 숙주 세포에 도입함으로써, 본원에 기재한 바와 같은 핵산에 의해 코딩되는 융합 단백질 또는 융합 펩티드 (예컨대 MP 단백질, MP 단백질의 돌연변이된 형태, 융합 단백질 등)를 비롯한 단백질 또는 펩티드를 제조할 수 있다.

본 발명의 재조합 발현 벡터는 원핵 세포 또는 진핵 세포에서 MP 단백질을 발현하도록 고안될 수 있다. 예를 들면, MP 유전자는 박테리아 세포, 예를 들어 코리네박테리움 글루타미쿰, 곤충 세포 (이 경우에는 바큘로바이러스 발현 벡터를 사용함), 효모 세포 및 기타 진균 세포 (문헌 [Romanos, M.A. et al. (1992) "Foreign gene expression in yeast: a review" Yeast 8: 423-488], [van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet ＆ L.L. Lasure, Editors, pp. 396-428: Academic Press: San Diego] 및 [van den Hondel, C.A.M.J.J. ＆ Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi" in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., Editors, pp. 1-28, Cambridge University Press: Cambridge] 참조), 조류(藻類) 및 다세포 식물 세포 (문헌 [Schmidt, R. and Willmitzer, L. (1988) "High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants", Plant Cell Rep.: 583-586] 참조) 또는 포유동물 세포에서 발현될 수 있다. 적합한 숙주 세포는 문헌 [Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990)]에 추가로 논의되어 있다. 별법으로, 재조합 발현 벡터는 예를 들어 T7 프로모터 조절 서열 및 T7 폴리머라제를 사용하여 시험관내 전사 및 번역시킬 수 있다.

단백질은 융합 또는 비-융합 단백질의 발현을 제어하는 구성적 또는 유도가능한 프로모터를 함유하는 벡터를 주로 사용하여 원핵생물에서 발현시킨다. 융합 벡터는 그에 의해 코딩되는 단백질에, 통상적으로는 재조합 단백질의 아미노 말단에 수많은 아미노산을 부가한다. 이러한 융합 벡터는 통상적으로 다음의 3가지 기능을 수행한다: 1) 재조합 단백질의 발현을 증진시킴; 2) 재조합 단백질의 가용성을 증가시킴; 3) 친화성 정제시에 리간드로서 작용하여 재조합 단백질의 정제를 보조함. 종종, 융합 발현 벡터에서 융합 단위와 재조합 단백질의 접합부에 단백질분해성 절단 부위를 도입함으로써, 재조합 단백질이 융합 단백질의 정제 후에 상기 융합 단위로부터 분리될 수 있도록 한다. 이러한 효소 및 이들의 상응하는 인식 서열은 인자 Xa, 트롬빈 및 엔테로키나제를 포함한다.

통상의 융합 발현 벡터는 재조합 표적 단백질에 글루타티온 S-트랜스퍼라제 (GST)가 융합된 pGEX (파마시아 바이오테크 인크. (Pharmacia Biotech Inc.) 제품, [Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40]), 재조합 표적 단백질에 말토스 E-결합 단백질이 융합된 pMAL (미국 매사추세츠주 배벌리에 소재하는 뉴 잉글랜드 바이오랩스 (New England Biolabs) 제품) 및 재조합 표적 단백질에 단백질 A가 융합된 pRIT 5 (미국 뉴저지주 피스카타웨이에 소재하는 파마시아 제품)를 포함한다. 한 실시양태에서, MP 단백질-코딩 서열을 pGEX 발현 벡터에 클로닝하여, 상기 벡터가 N-말단에서 C-말단 방향으로 GST-트롬빈 절단 부위-단백질 X를 포함하는 융합 단백질을 코딩하도록 한다. 융합 단백질은 글루타티온-아가로스 수지를 사용한 친화성 크로마토그래피로 정제할 수 있다. GST에 융합되지 않은 재조합 MP 단백질은 융합 단백질을 트롬빈으로 절단하여 수득할 수 있다.

적합한 유도가능한 비-융합 대장균 발현 벡터의 예로는 pTrc [Amann et al., (1988) Gene 69:301-315] 및 pET 11d [Studier et al. Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89] 등이 있다. pTrc 벡터로부터의 표적-유전자 발현은 숙주 RNA 폴리머라제에 의한 하이브리드 trp-lac 융합 프로모터로부터의 전사를 기초로 한다. pET 11d 벡터로부터의 표적-유전자 발현은 동시발현되는 바이러스 RNA 폴리머라제 (T7 gn1)에 의해 매개되는 T7-gn10-lac 융합 프로모터로부터의 전사를 기초로 한다. BL21 (DE3) 또는 HMS174 (DE3) 숙주 균주에서, 상기 바이러스 폴리머라제는 lacUV 5 프로모터의 전사 제어를 받는 T7 gn1 유전자를 보유하는 상재 (resident) λ 프로파지에 의해 제공된다.

재조합 단백질의 발현을 최대화하기 위한 전략 중 하나는 상기 재조합 단백질을 그에 대한 단백질분해성 절단력이 파괴된 숙주 박테리아에서 발현시키는 것이다 [Gottesman, S. Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128]. 또다른 전략은 발현 벡터로 삽입될 핵산의 핵산 서열을 변형시켜 각 아미노산에 대한 개개의 코돈이 발현을 위해 선택한 박테리아, 예를 들어 코리네박테리움 글루타미쿰에서 바람직하게 이용되는 코돈이 되도록 하는 것이다 [Wada et al. (1992) Nucleic Acids Res. 20:2111-2118]. 본 발명의 핵산 서열에 대한 이러한 변형은 DNA 합성의 표준 기술을 이용하여 수행할 수 있다.

추가의 실시양태에서, MP 단백질 발현 벡터는 효모 발현 벡터이다. 효모 사카로마이세스 세레비지애 (Saccharomyces cerevisiae)에서 발현시키기 위한 벡터의 예로는 pYepSec1 [Baldari et al., (1987) Embo J. 6:229-234], pMFa [Kurjan and Herskowitz (1982) Cell 30:933-943], pJRY88 [Schultz et al. (1987) Gene 54:113-123] 및 pYES2 (미국 캘리포니아주 샌 디에고에 소재하는 인비트로젠 코포레이션 (Invitrogen Corporation) 제품) 등이 있다. 다른 진균, 예를 들어 섬유상 진균에 사용하기에 적합한 벡터 및 그의 제작 방법은 문헌 [van den Hondel, C.A.M.J.J. ＆ Punt, P.J. (1991) "Gene Transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, J.F. Peberdy et al., Editors, pp. 1-28, Cambridge University Press: Cambridge]에 상세하게 기재된 것들을 포함한다.

별법으로, 본 발명의 MP 단백질은 바큘로바이러스 발현 벡터를 사용한 곤충 세포에서 발현될 수도 있다. 배양된 곤충 세포 (예컨대 Sf9 세포)에서의 단백질 발현에 이용가능한 바큘로바이러스 벡터로는 pAc 계열 [Smith et al., (1983) Mol. Cell Biol. 3:2156-2165] 및 pVL 계열 [Lucklow and Summers (1989) Virology 170:31-39] 등이 있다.

추가의 실시양태에서, 본 발명의 MP 단백질은 단세포 식물 세포 (예컨대 조류) 또는 고등 식물 (예컨대 작물 등의 종자식물) 세포에서 발현될 수 있다. 식물 발현 벡터의 예로는 문헌 ([Becker, D., Kemper, E., Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20:1195-1197] 및 [Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acids Res. 12:8711-8721])에 상세하게 기재된 것들을 포함한다.

또다른 실시양태에서, 본 발명의 핵산 발현을 포유동물 발현 벡터를 사용한 포유동물 세포에서 수행한다. 포유동물 발현 벡터의 예로는 pCDM8 [Seed, B. (1987) Nature 329:840] 및 pMT2PC [Kaufman et al. (1987) EMBO J. 6:187-195] 등이 있다. 포유동물 세포에서 사용할 경우, 발현 벡터의 제어 기능은 종종 바이러스 조절 요소에 의해 제공된다. 통상 사용되는 프로모터는 예를 들어 폴리오마, 아데노바이러스 2, 사이토메갈로바이러스 및 시미안 바이러스 40에서 유래된 것이 다. 원핵 세포 및 진핵 세포에 적합한 다른 발현 시스템에 대해서는 문헌 [Sambrook, J., Fritsch, E.F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2^nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989]의 제16장 및 제17장에서 찾을 수 있다.

추가의 실시양태에서, 재조합 포유동물 발현 벡터는 바람직하게는 특정 세포 유형에서 핵산을 발현시킬 수 있다 (예를 들어, 조직-특이적 조절 요소를 사용하여 핵산을 발현시킴). 조직-특이적 조절 요소는 당업계에 공지되어 있다. 적합한 조직-특이적 프로모터의 예로는 알부민 프로모터 (간-특이적; [Pinkert et al. (1987) Genes De. 1:268-277]), 림프양-특이적 프로모터 [Calame and Eaton (1988) Adv. Immunol. 43:235-275], 특히 T-세포 수용체의 프로모터 [Winoto and Baltimore (1989) EMBO J. 8:729-733] 및 이뮤노글로불린의 프로모터 ([Banerji et al. (1983) Cell 33:729-740], [Queen and Baltimore (1983) Cell 33:741-748]), 뉴런-특이적 프로모터 (예컨대 신경필라멘트 프로모터; [Byrne and Ruddle (1989) PNAS 86:5473-5477]), 췌장-특이적 프로모터 [Edlund et al., (1985) Science 230:912-916] 및 유선(乳腺)-특이적 프로모터 (예컨대 유장(乳漿) (milk serum) 프로모터; 미국 특허 제4,873,316호 및 유럽 특허 출원 번호 제264,166호) 등이 있으나 이에 제한되지 않는다. 또한, 발생-조절되는 프로모터의 예로는 뮤린 (murine) hox 프로모터 [Kessel and Gruss (1990) Science 249:374-379] 및 α-태아단백질 프로모터 [Campes and Tilghman (1989) Genes Dev. 3:537-546] 등이 있다.

추가로, 본 발명은 발현 벡터에 안티센스 방향으로 클로닝된 본 발명의 DNA 분자를 포함하는 재조합 발현 벡터를 제공한다. 이는 DNA 분자가 조절 서열에 기능적으로 연결되어 MP mRNA에 대해 안티센스인 RNA 분자가 발현 (DNA 분자의 전사를 통해 발현됨)될 수 있음을 의미한다. 안티센스 방향으로 클로닝된 핵산에 기능적으로 결합되고 다수의 세포 유형에서 안티센스 RNA 분자가 지속적으로 발현되도록 제어하는 조절 서열을 선택할 수 있으며, 예를 들어 안티센스 RNA가 구성적으로 조직-특이적 발현되거나 세포 유형-특이적 발현되도록 제어하는 바이러스 프로모터 및(또는) 인핸서 또는 조절 서열을 선택할 수 있다. 안티센스 발현 벡터는 재조합 플라스미드, 파지미드 또는 약독화 바이러스의 형태일 수 있으며, 이는 벡터가 도입된 세포 유형에 따라 활성이 결정되는 고도로 효과적인 조절 영역의 제어하에서 안티센스 핵산을 생산한다. 안티센스 유전자를 이용한 유전자 발현의 조절에 관해서는 문헌 [Weintraub, H. et al., Antisense-RNA as a molecular tool for genetic analysis, Reviews - Trends in Genetics, Vol. 1(1) 1986]에 논의되어 있다.

본 발명의 또다른 측면은 본 발명의 재조합 발현 벡터가 도입된 숙주 세포에 관한 것이다. 본원에서는 용어 "숙주 세포" 및 "재조합 숙주 세포"를 혼용하여 사용한다. 사실상, 이러한 용어는 특정 표적 세포와만 관련된 것이 아니라 그 세포의 자손 또는 잠재적인 자손과도 관련된 것이다. 돌연변이 또는 환경적 요인으로 인해서 특정 변형이 세대에 걸쳐 연속적으로 나타날 수 있기 때문에, 이 자손이 모 (母) 세포와 반드시 동일한 것은 아니지만, 본원에서 사용된 바와 같이 상기 용어의 범위에 포함되는 것은 마찬가지이다.

숙주 세포는 원핵 세포이거나 진핵 세포일 수 있다. 예를 들면, MP 단백질은 박테리아 세포, 예를 들어 코리네박테리움 글루타미쿰, 곤충 세포, 효모 또는 포유동물 세포 (예컨대 차이니스 햄스터 난소 (CHO) 세포 또는 COS 세포)에서 발현될 수 있다. 다른 적합한 숙주 세포는 당업자에게 공지되어 있다. 코리네박테리움 글루타미쿰과 관련되어 있고 적합한 방식으로 본 발명의 핵산 분자 및 단백질 분자를 위한 숙주 세포로서 사용될 수 있는 미생물은 표 3에 기재되어 있다.

통상의 형질전환 또는 형질감염 방법을 이용하여 벡터 DNA를 원핵 세포 또는 진핵 세포에 도입할 수 있다. 본원에서 사용된 바와 같이, 용어 "형질전환" 및 "형질감염"은 외래 핵산 (예컨대 DNA)을 숙주 세포에 도입하기 위한 인산칼슘 또는 염화칼슘 동시침전법, DEAE-덱스트란-매개된 형질감염법, 리포펙션법 (lipofection) 또는 전기천공법 등과 같은 당업계에 공지된 다수의 방법을 포함한다. 숙주 세포의 형질전환 또는 형질감염에 적합한 방법은 문헌 [Sambrook et al., Molecular Cloning: A Laboratory Manual. 2^nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989] 및 다른 실험 안내서에서 찾을 수 있다.

포유동물 세포의 안정한 형질감염의 경우, 사용된 발현 벡터 및 이용된 형질감염 기술에 따라 오직 적은 비율의 세포만이 외래 DNA를 그의 게놈에 통합시킬 수 있음이 공지되어 있다. 통상적으로, 이러한 통합체는 선별가능한 마커 (예컨대 항생제에 대한 내성)를 코딩하는 유전자를 관심 유전자와 함께 숙주 세포에 도입함으로써 확인 및 선별된다. 바람직한 선별가능한 마커로는 G418, 하이그로마이신 및 메토트렉세이트 등과 같은 약물에 내성을 부여하는 유전자 등이 있다. 선별가능한 마커를 코딩하는 핵산을 MP 단백질을 코딩하는 벡터에 함께 함유시켜서 숙주 세포에 도입하거나, 상기 벡터와는 별개인 벡터를 통해 숙주 세포에 도입할 수 있다. 안정하게 형질감염되어 핵산이 도입된 세포는 예를 들어 약물 선별을 통해 확인할 수 있다 (예를 들어 선별가능한 마커가 통합된 세포는 생존하지만 다른 세포는 사멸함).

상동성 재조합된 미생물을 생성하기 위해서는, MP 유전자에 결실, 부가 또는 치환을 도입하여 그를 변형시킨, 예를 들어 기능적으로 파괴시킨 하나 이상의 MP-유전자 절편을 함유하는 벡터를 제조한다. 상기 MP 유전자는 코리네박테리움 글루타미쿰 MP 유전자인 것이 바람직하지만, 그와 관련된 박테리아 또는 심지어는 포유동물, 효모 또는 곤충 공급원으로부터의 상동체를 사용할 수도 있다. 바람직한 실시양태에서, 벡터는 상동성 재조합이 내인성 MP 유전자를 기능적으로 파괴 (즉, 유전자가 더이상 기능적 단백질을 코딩하지 않게됨; "녹아웃 (knockout) 벡터"라고 지칭하기도 함)하도록 고안될 수 있다. 별법으로, 벡터는 상동성 재조합 돌연변이체 등이 내인성 MP 유전자를 변형시키지만 여전히 기능적 단백질을 코딩하도록 고안될 수 있다 (예를 들어, 상류에 위치한 조절 영역을 변형시킴으로써 내인성 MP 단백질의 발현이 변형되도록 할 수 있음). 상동성 재조합 벡터 내에 존재하는 변 형된 MP-유전자 절편의 5' 및 3' 말단에는 MP 유전자의 추가 핵산이 플랭킹되어 있는데, 이는 벡터가 운반하는 외인성 MP 유전자와 미생물의 내인성 MP 유전자 사이의 상동성 재조합을 가능케 한다. 추가의 플랭킹 MP 핵산은 내인성 유전자와의 성공적인 상동성 재조합에 충분한 길이이다. 통상적으로, 벡터는 플랭킹 DNA를 1 kb 미만으로 함유한다 (5' 말단 및 3' 말단 모두에서) (상동성 재조합 벡터에 관해서는 예를 들어 문헌 [Thomas, K.R. and Capecchi, M.R. (1987) Cell 51:503]의 기재 참조). 벡터를 미생물에 도입 (예를 들어 전기천공법에 의해 도입)하고, 도입된 MP 유전자가 내인성 MP 유전자와 상동성 재조합된 세포를 당업계에 공지된 방법으로 선별한다.

또다른 실시양태에서, 도입된 유전자의 발현을 조절할 수 있는 것으로 선택한 시스템을 함유하는 재조합 미생물을 생산할 수 있다. 벡터로의 MP 유전자 삽입은 상기 유전자가 lac 오페론의 제어를 받게 하기 때문에, 예를 들어 MP-유전자 발현이 IPTG의 존재하에서만 가능해 진다. 이러한 조절 시스템은 당업계에 공지되어 있다.

본 발명의 숙주 세포, 예를 들어 배양된 원핵 세포 또는 진핵 숙주 세포를 사용하여 MP 단백질을 생산할 수 있다 (즉, 발현시킬 수 있음). 추가로, 본 발명은 본 발명의 숙주 세포를 이용한 MP 단백질의 생산 방법을 제공한다. 한 실시양태에서, 상기 방법은 본 발명의 숙주 세포 (MP 단백질을 코딩하는 재조합 발현 벡터가 도입되어 있거나 그의 게놈에 야생형 또는 변형된 MP 단백질을 코딩하는 유전자가 도입되어 있음)를 적합한 배지 중에서 MP 단백질이 생산될 때까지 배양하는 단계를 포 함한다. 추가의 실시양태에서, 상기 방법은 상기 배지 또는 숙주 세포로부터 MP 단백질을 단리하는 단계를 포함한다.

C. 본 발명의 용도 및 방법

본원에 기재한 핵산 분자, 단백질, 단백질 상동체, 융합 단백질, 프라이머, 벡터 및 숙주 세포는 하기한 방법 중 하나 이상에서 사용할 수 있다: 코리네박테리움 글루타미쿰 및 그와 관련된 유기체의 확인, 코리네박테리움 글루타미쿰과 관련이 있는 유기체의 게놈 맵핑, 코리네박테리움 글루타미쿰 관심 서열의 확인 및 위치분석, 진화 연구, 기능수행에 필요한 MP 단백질 영역의 결정, MP 단백질의 활성 조정; MP 경로의 활성 조정; 및 관심 화합물, 예컨대 정밀 화학물질의 세포내 생산 조정. 본 발명의 MP 핵산 분자에는 여러가지 용도가 있다. 첫째로, 이들은 코리네박테리움 글루타미쿰 또는 그와 밀접한 관련이 있는 유기체의 확인에 이용될 수 있다. 또한, 이들은 미생물들이 혼합된 집단 내에서 코리네박테리움 글루타미쿰 또는 그와 관련된 유기체를 확인하는데 사용될 수 있다. 본 발명은 매우 다양한 코리네박테리움 글루타미쿰 유전자의 핵산 서열을 제공한다. 1종 미생물의 집단 또는 미생물들이 혼합된 집단의 배양물에서 추출한 게놈 DNA를 엄격 조건하에서 코리네박테리움 글루타미쿰에 고유한 유전자 영역을 포함하는 프로브로 프로빙 (probing)함으로써 상기 유기체의 존재 여부를 결정할 수 있다. 코리네박테리움 글루타미쿰 자체는 병원성이 아니지만, 코리네박테리움 디프테리애 등과 같은 병원성 종과 관련이 있다. 이러한 유기체의 검출은 실질적인 임상적 중요성을 갖는다.

본 발명의 핵산 및 단백질 분자는 게놈의 특정 영역에 대한 마커로 작용할 수 있다. 본 발명의 핵산 및 단백질 분자는 게놈 맵핑 뿐 아니라 코리네박테리움 글루타미쿰 단백질의 기능 연구에도 유용하다. 특정 코리네박테리움 글루타미쿰 DNA-결합 단백질이 결합하는 게놈 영역은 예를 들어 코리네박테리움 글루타미쿰 게놈을 절단하고 그 단편들을 DNA-결합 단백질과 함께 인큐베이션 시켜서 확인할 수 있다. 상기 단백질과 결합하는 단편은 본 발명의 핵산 분자로 바람직하게는 쉽게 검출가능한 표지를 이용하여 추가로 프로빙될 수 있다; 이러한 핵산 분자와 게놈 단편의 결합을 통해, 코리네박테리움 글루타미쿰의 게놈 맵에서 상기 단편의 위치를 결정할 수 있으며 여러가지 효소를 사용하여 이 방법을 여러 회 수행함으로써, 단백질이 결합하는 핵산 서열의 신속한 결정을 용이하게 할 수 있다. 추가로, 본 발명의 핵산 분자는 관련 종의 서열에 충분한 상동성이 있을 수 있으며, 이 경우에도 이러한 핵산 분자를 마커로서 사용하여 해당 박테리아 (예컨대 브레비박테리움 락토페르멘툼)의 게놈 맵을 제작할 수 있다.

또한, 본 발명의 MP 핵산 분자는 진화 연구 및 단백질 구조 연구에도 적합하다. 여러 원핵 세포 및 진핵 세포는 본 발명의 분자가 관여하는 대사 과정을 이용한다. 본 발명의 핵산 분자 서열을 다른 유기체에서의 유사 효소를 코딩하는 핵산 분자 서열과 비교함으로써 상기 유기체의 진화 관계 정도를 조사할 수 있다. 따라서, 이러한 비교를 통해 서열 영역이 보존되었는지의 여부를 결정할 수 있고, 이는 효소 기능에 필수적인 단백질의 보존 영역을 조사하는데 도움을 줄 수 있다. 이러한 방식의 조사는 단백질 조작 연구에 중요하며, 단백질에서 그의 기능 손실 없이 돌연변이유발을 허용할 수 있는 수준에 관한 지침을 제공할 수 있다.

본 발명의 MP 핵산 분자의 유전자 조작을 통해, 야생형 MP 단백질과는 기능적으로 상이한 MP 단백질이 생산되도록 할 수 있다. 이러한 단백질은 효율 또는 활성과 관련하여 개선될 수 있어서, 이들이 세포 내에 통상적인 수보다 더 많은 수로 존재하게 하거나 이들의 효율 또는 활성을 약화시킬 수도 있다.

이러한 활성 변화를 통해 코리네박테리움 글루타미쿰에서 1종 이상의 정밀 화학물질의 수율, 생산량 및(또는) 생산 효율을 직접 조정할 수 있다. 예를 들면, 관심 정밀 화학물질의 생합성의 단백질을 코딩하는 유전자의 전사 또는 번역을 활성화시키는 MP 단백질의 활성을 최적화시키거나, 이러한 유전자의 전사 또는 번역을 억제하는 MP 단백질의 활성에 영향을 주거나 이 활성을 삭제함으로써, 예를 들어 속도제한 효소가 증가된 양으로 존재하므로 상기 생합성 경로의 활성 또는 활성 속도가 증가될 수 있다. 상응하게, 관심 정밀 화학물질의 분해 경로에 관여하는 단백질을 구조적으로 후번역적으로 비활성화시키도록 MP 단백질의 활성을 변형시킴으로써 또는 이러한 유전자의 전사 또는 번역을 구조적으로 억제하도록 MP 단백질의 활성을 변형시킴으로써, 화합물의 분해가 감소하므로 세포로부터 상기 정밀 화학물질의 수율 및(또는) 생산 속도가 증가될 수 있다.

각종 대사 경로가 연결되어 있기 때문에, 1종 이상의 MP 단백질의 활성을 조정함으로써 세포로부터의 1종 이상의 정밀 화학물질 생산을 간접적으로 촉진시키거나 생산 속도를 개선시키는 것이 가능해진다. 예를 들어, 리신 생합성에서의 1종 이상의 효소의 발현을 활성화하여 수율, 생산량 및(또는) 생산 효율을 증가시킴으로써, 더 많은 양의 리신이 요구되는 경우 세포가 보통 더 많은 양을 필요로 하는 다른 아미노산과 같은 다른 화합물의 발현을 동시에 증가시키는 것이 가능하다. 발효 배양의 환경 조건 (영양소 및 산소의 공급이 불량할 수 있고, 독성 폐기물이 환경에 다량 존재할 수 있다고 추정됨)하의 세포가 성장 또는 복제를 개선시키도록 전체적인 세포에서의 대사 조절을 변형시키는 것이 또한 가능하다. 따라서, 성장 조건이 최적에 이르지 못할 지라도, 반응 중 세포막 생산에 요구되는 분자의 합성을 억제하는 MP 단백질을 세포외 매질 중의 고수준의 폐기물로 돌연변이시킴으로써 (최적에 이르지 못하는 성장 조건에서 세포 성장 및 세포 분열을 차단하기 위함) 상기 단백질이 더이상 상기 합성을 억제할 수 없게 하도록 배양중 세포의 성장 및 전파를 개선시키는 것 등이 가능하다. 배양물 중에는 상기 화합물을 생산하는 세포가 비교적 다수 존재하기 때문에, 이러한 증가된 성장 또는 증가된 생존력은 또한 발효 배양으로부터의 관심 정밀 화학물질의 수율 및(또는) 생산 속도를 증가시켜야 한다.

코리네박테리움 글루타미쿰으로부터의 정밀 화학물질 수율을 증가시키기 위해서 MP 단백질을 돌연변이유발시키는 상기 전략들은 그에 제한되는 것이 아니며, 당업자에게는 이러한 돌연변이유발 전략들에 대한 변형법이 매우 명백하다. 이러한 전략을 사용하고 본원에 개시한 메카니즘을 포함시킴으로써, 돌연변이된 MP 핵산 및 단백질 분자를 발현하는 코리네박테리움 글루타미쿰 또는 그와 관련된 박테리아 균주를 생성하여 관심 화합물의 수율, 생산량 및(또는) 생산 효율을 개선시키는데 본 발명의 핵산 및 단백질 분자를 이용할 수 있다. 상기 관심 화합물은, 생합성 경로의 최종 생성물 및 천연 대사 경로의 중간체 뿐 아니라 코리네박테리움 글루타미쿰 대사시에 천연적으로 생성되는 것은 아니지만 본 발명의 코리네박테리움 글루타미쿰 균주에 의해 생산되는 분자 등을 비롯하여 코리네박테리움 글루타미쿰에 의해 생산된 임의의 생성물일 수 있다.

하기 실시예는 본 발명을 추가로 예시하기 위한 것으로써, 본 발명을 제한하는 것으로 이해해서는 안된다. 본 명세서에서 인용한 모든 참고문헌, 특허 출원 명세서, 특허 문헌 및 공개된 특허 출원 명세서의 내용은 본원에 참고로 도입된다.

실시예 1: 코리네박테리움 글루타미쿰 ATCC13032의 전체 게놈 DNA의 프렙

BHI 배지 (디프코 (Difco) 제품) 중의 코리네박테리움 글루타미쿰 (ATCC 13032) 배양물을 30℃에서 격렬히 진탕시키면서 밤새 배양하였다. 원심분리하여 세포를 회수하여 상층액은 버리고, 세포를 완충액-I 5 mL (배양물 처음 부피의 5％에 해당함-언급한 모든 부피는 배양 부피 100 mL에 대해 계산한 값임) 중에 재현탁했다. 완충액-I의 조성은 다음과 같았다: 140.34 g/L 수크로스, 2.46 g/L MgS0₄ ×7 H₂O, 10 mL/L KH₂PO₄ 용액 (100 g/L, KOH를 사용하여 pH 6.7로 조정함), 50 mL/L M12 농축물 (10 g/L (NH₄)₂SO₄, 1 g/L NaCl, 2 g/L MgSO₄ ×7 H₂O, 0.2 g/L CaCl₂, 0.5 g/L 효모 추출물 (디프코 제품), 10 mL/L 미량 원소 혼합물 (200 mg/L FeSO₄ ×H₂O, 10 mg/L ZnSO₄ ×7 H₂O, 3 mg/L MnCl₂ ×4 H₂O, 30 mg/L H₃BO₃, 20 mg/L CoCl₂ ×6 H₂O, 1 mg/L NiCl₂ ×6 H₂O, 3 mg/L Na₂MoO₄ ×2 H₂O), 500 mg/L 착화제 (EDTA 또는 시트르산), 100 mL/L 비타민 혼합물 (0.2 mg/L 바이오틴, 0.2 mg/L 엽산, 20 mg/L p-아미노 벤조산, 20 mg/L 리보플라빈, 40 mg/L Ca 판토테네이트, 140 mg/L 니코틴산, 40 mg/L 피리독솔 히드로클로라이드, 200 mg/L 미오이노시톨). 상기 현탁액에 리소짐을 최종 농도 2.5 mg/mL로 첨가했다. 37℃에서 대략 4시간 동안 인큐베이션시킨 후, 세포벽을 파괴하고 원심분리하여 원형질체를 수거했다. 펠렛을 완충액-I 5 mL로 1회 세척하고, TE 완충액 (10 mM Tris-HCl, 1 mM EDTA, pH 8) 5 mL로 1회 세척했다. 펠렛을 TE 완충액 4 mL 중에 재현탁하고, SDS 용액 (10％) 0.5 mL 및 NaCl 용액 (5 M) 0.5 mL을 첨가했다. 프로테이나제 K를 최종 농도 200 ㎍/mL로 첨가한 후, 상기 현탁액을 37℃에서 대략 18시간 동안 인큐베이션시켰다. 표준 방법에 따라 DNA를 페놀, 페놀/클로로포름/이소아밀 알콜 및 클로로포름/이소아밀 알콜로 추출하여 정제했다. 이어서, 1/50배 부피의 3 M 아세트산나트륨 및 2배 부피의 에탄올을 첨가하여 DNA를 침전시킨 후, -20℃에서 30분 동안 인큐베이션시키고, SS34 회전자 (소르발 (Sorvall) 제품)를 사용한 고속 원심분리기에서 12,000 rpm로 30분 동안 원심분리했다. DNA를 20 ㎍/mL RNaseA를 함유하는 TE 완충액 1 mL 중에 용해시키고, 4℃에서 3시간 이상 동안 TE 완충액 1000 mL 중에 투석했다. 투석 동안, 상기 완충액을 3회 교환하였다. 투석된 DNA 용액을 0.4 mL씩 분취하여, 2 M LiCl 0.4 mL 및 에탄올 0.8 mL을 첨가했다. -20℃에서 30분 동안 인큐베이션시킨 후, 원심분리 (13,000 rpm, 독일 하나우 헤래우스에 소재하는 바이오퓨지 프레스코 (Biofuge Fresco) 제품)하여 DNA를 수집했다. DNA 펠렛을 TE 완충액 중에 용해했다. 이 방법으로 프렙된 DNA를 서던 블럿팅 또는 게놈 뱅크 제작 등의 모든 목적 에 이용했다.

실시예 2: 대장균에서 코리네박테리움 글루타미쿰 (ATCC13032)의 게놈 뱅크 제작

실시예 1에 기재한 바와 같이 프렙된 DNA를 사용하여, 공지되고 수립되어 있는 방법 (예를 들어 문헌 [Sambrook, J. et al. (1989) "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press] 또는 문헌 [Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley ＆ Sons] 참조)에 따라 코스미드 및 플라스미드 뱅크를 제작했다.

임의의 플라스미드 또는 코스미드를 사용할 수 있었다. 특히, 플라스미드 pBR322 [Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741]; pACYC177 [Change ＆ Cohen (1978) J. Bacteriol 134:1141-1156], pBS 계열의 플라스미드 (pBSSK+, pBSSK- 등; 미국 라졸라에 소재하는 스트라타진 (Stratagene) 제품) 또는 SuperCos I (미국 라졸라에 소재하는 스트라타진 제품) 또는 Lorist6 [Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286] 등의 코스미드를 사용하는 것이 바람직했다.

실시예 3: DNA 서열분석 및 컴퓨터를 이용한 기능 분석

실시예 2에 기재한 바와 같은 게놈 뱅크를 사용하여, 표준 방법, 특히 ABI377 서열 분석기를 사용한 쇄 종결 방법 (예를 들어 문헌 [Fleischman, R.D. et al. (1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd.", Science, 269:496-512])에 따른 DNA 서열분석을 수행했다. 하기 뉴클레오티드 서열을 갖는 서열분석 프라이머를 사용했다: 5'-GGAAACAGTATGACCATG- 3' 또는 5'-GTAAAACGACGGCCAGT-3'.

실시예 4: 생체내 돌연변이유발

코리네박테리움 글루타미쿰의 생체내 돌연변이유발은 유전 정보의 통합성을 유지할 수 없는 대장균 또는 다른 미생물 (예를 들면, 바실러스 종 또는 사카로마이세스 세레비지애 등의 효모)에 플라스미드 (또는 다른 벡터) DNA를 계대배양하여 수행할 수 있다. 전형적인 돌연변이유발 균주는 DNA 복구 시스템에 관한 유전자 (예를 들면, mutHLS, mutD, mutT 등; 비교하기 위해서는 문헌 [Rupp, W.D. (1996) DNA repair mechanisms, in Escherichia coli and Salmonella, p.2277-2294, ASM: Washington] 참조)에 돌연변이가 있다. 이러한 균주들은 당업자에게 공지되어 있다. 이러한 균주의 사용법은 예를 들어 문헌 [Greener, A. and Callahan, M. (1994) Strategies 7:32-34]에 예시되어 있다.

실시예 5: 대장균과 코리네박테리움 글루타미쿰 사이의 DNA 전달

여러 코리네박테리움 및 브레비박테리움 종은 자율적으로 복제되는 내인성 플라스미드 (예를 들면, pHM1519 또는 pBL1 등)를 함유하고 있다 (이에 관해 검토하기 위해서는 예를 들어 문헌 [Martin, J.F. et al. (1987) Biotechnology, 5:137-146] 참조). 대장균 및 코리네박테리움 글루타미쿰에 사용하기 위한 셔틀 벡터는 대장균의 경우에 사용하는 표준 벡터 (문헌 [Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press] 또는 문헌 [Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley ＆ Sons])에 코리네박테리움 글루타미쿰의 복제 기점 및 적 당한 마커를 부가하여 사용함으로써 쉽게 제작될 수 있다. 이러한 복제 기점은 코리네박테리움 및 브레비박테리움 종에서 단리한 내인성 플라스미드로부터 얻는 것이 바람직하다. 이들 종에 특히 유용한 형질전환 마커는 카나마이신 내성 유전자 (예를 들면, Tn5 또는 Tn903 트란스포손에서 유래함) 또는 클로람페니콜 내성 유전자 [Winnacker, E.L. (1987) "From Genes to Clones-Introduction to Gene Technology", VCH, Weinheim]이다. 대장균 및 코리네박테리움 글루타미쿰에서 복제되고 유전자 과다발현 등을 비롯한 각종 목적에 사용할 수 있는 매우 다양한 셔틀 벡터의 제조법에 관한 문헌들이 많이 있다 (예를 들어 [Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597], [Martin J.F. et al. (1987) Biotechnology, 5:137-146] 및 [Eikmanns, B.J. et al. (1991) Gene, 102:93-98] 참조).

표준 방법을 이용하여, 관심 유전자를 상기 기재한 셔틀 벡터들 중 하나에 클로닝하고 이러한 하이브리드 벡터를 코리네박테리움 글루타미쿰 균주에 도입할 수 있다. 코리네박테리움 글루타미쿰은 원형질체 형질전환법 [Kastsumata, R. et al. (1984) J. Bacteriol. 159:306-311], 전기천공법 [Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303]을 이용하고 특정 벡터가 사용되는 경우에는 접합법 (예를 들어 문헌 [Schaefer, A et al. (1990) J. Bacteriol. 172:1663-1666]에 기재된 바와 같음)도 이용하여 형질전환시킬 수 있다. 또한, 코리네박테리움 글루타미쿰으로부터 플라스미드 DNA를 프렙 (당업계에 공지된 표준 방법을 사용함)하고, 이것으로 대장균을 형질전환시켜 코리네박테리움 글루타미쿰의 셔틀 벡터를 대장균에 전달할 수도 있다. 이러한 형질전환 단계는 표준 방법으로 수행할 수 있지만, NM522 [Gough ＆ Murray (1983) J. Mol. Biol. 166:1-19] 등과 같은 Mcr-결핍 대장균 균주를 사용하는 것이 유리하고 바람직하다.

실시예 6: 돌연변이된 단백질의 발현 평가

형질전환된 숙주 세포에서 돌연변이된 단백질의 활성 관찰은 상기 돌연변이 단백질이 야생형 단백질과 유사한 방식 및 유사한 양으로 발현된다는 사실을 바탕으로 한다. 돌연변이된 유전자의 전사량 (유전자 생성물의 번역에 이용가능한 mRNA 양에 관한 지표) 측정에 적합한 방법은 관심 유전자에 결합하도록 고안되어 검출가능한 표지 (통상적으로 방사성 표지 또는 화학발광 표지)가 부착된 프라이머를 사용하여 노던 블럿팅 (예를 들어 문헌 [Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York] 참조)을 수행하는 것인데, 이는 유기체 배양물의 전체 RNA를 추출하고 겔상에서 분리하여 안정한 매트릭스에 옮긴 후에 상기 프로브와 인큐베이션시켜, 프로브의 결합 및 그 결합 활성이 상기 유전자에 대한 mRNA의 존재 여부 및 또한 그 존재량을 지시하는 방법이다. 이러한 정보는 돌연변이된 유전자의 전사 정도에 관한 지표가 된다. 예를 들어 문헌 [Bormann, E.R. et al. (1992) Mol. Microbiol. 6:317-326]에 기재된 것과 같이 당업계에 공지된 각종 방법들을 이용하여 코리네박테리움 글루타미쿰의 세포내 전체 RNA를 단리할 수 있다.

상기 mRNA로부터 번역된 단백질의 존재 여부 또는 그 상대량은 웨스턴 블럿팅과 같은 표준 기술을 사용하여 측정할 수 있다 (예를 들어 문헌 [Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York] 참조). 이 방 법에서는, 세포내 전체 단백질을 추출하여 겔 전기영동법으로 분리한 후에 이를 니트로셀룰로스 등과 같은 매트릭스에 옮겨서 관심 단백질에 특이적으로 결합하는 항체 등과 같은 프로브와 함께 인큐베이션시킨다. 통상적으로, 이 프로브에는 쉽게 검출될 수 있는 화학발광 표지 또는 비색 표지가 부착되어 있다. 표지의 존재 여부 및 그의 관찰된 양은 그 세포 내에서 원하는 돌연변이 단백질의 존재 여부 및 그 존재량을 지시한다.

실시예 7: 유전자 변형된 코리네박테리움 글루타미쿰의 성장 - 배지 및 배양 조건

유전자 변형된 코리네박테리움을 합성 또는 천연 성장 배지에서 배양한다. 코리네박테리움을 위한 여러 다양한 성장 배지는 당업계에 공지되어 있으며 쉽게 입수가능하다 (문헌 [Lieb et al. (1989) Appl. Microbiol. Biotechnol. 32:205-210], [von der Osten et al. (1998) Biotechnology Letters, 11:11-16], 독일 특허 제4,120,867호, [Liebl (1992) "The Genus Corynebacterium", in: The Procaryotes, Volume II, Balows, A. et al., eds. Springer-Verlag]). 이들 배지는 1종 이상의 탄소 공급원, 질소 공급원, 무기염, 비타민 및 미량 원소로 구성된다. 바람직한 탄소 공급원은 단당류, 이당류 또는 다당류 등의 당이다. 매우 훌륭한 탄소 공급원의 예로는 글루코스, 프럭토스, 만노스, 갈락토스, 리보스, 소르보스, 리불로스, 락토스, 말토스, 수크로스, 라피노스, 녹말 및 셀룰로스 등이 있다. 또한, 당 정제시 생성되는 당밀 또는 기타 부산물 등과 같은 복합 화합물 등으로서 배지에 당을 공급할 수도 있다. 또한, 각종 탄소 공급원들의 혼합물을 첨가하는 것이 유리할 수도 있다. 다른 가능한 탄소 공급원은 메탄올, 에탄올, 아세 트산 또는 락트산 등의 알콜 및 유기산이다. 질소 공급원은 통상적으로 유기 또는 무기 질소 화합물이거나 이러한 화합물을 함유하는 물질이다. 질소 공급원의 예로는 암모니아 기체 및 NH₄Cl 또는 (NH₄)₂SO₄, NH₄0H 등의 암모늄염, 질산염, 우레아, 아미노산 또는 옥수수 침유 (cornsteep liquor), 대두 가루, 대두 단백질, 효모 추출물, 육류 추출물 등과 같은 복합 질소 공급원 등이 있다.

배지에 함유시킬 수 있는 무기 염 화합물로는 칼슘, 마그네슘, 나트륨, 코발트, 몰리브덴, 칼륨, 망간, 아연, 구리 및 철의 염산염, 인산염 또는 황산염 등이 있다. 킬레이팅제를 배지에 첨가하여 금속 이온을 용액 중에 유지할 수 있다. 특히 적합한 킬레이팅제로는 카테콜 또는 프로토카테쿠에이트 등과 같은 디히드록시페놀 또는 시트르산 등의 유기산 등이 있다. 또한, 배지에는 통상적으로 비타민 또는 성장 촉진제 등의 다른 성장 인자가 함유되어 있고, 이들의 예로는 바이오틴, 리보플라빈, 티아민, 엽산, 니코틴산, 판토테네이트 및 피리독신 등이 있다. 성장 인자 및 염은 흔히 효모 추출물, 당밀, 옥수수 침유 등과 같은 복합 배지 성분으로부터 생성된다. 배지의 정확한 조성은 특정 실험마다 크게 달라지고, 각 경우마다 따로 결정된다. 배지의 최적화에 관한 정보는 문헌 ["Applied Microbiol. Physiology, A Practical Approach (editors P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp.53-73, ISBN 0 19 963577 3)]에서 찾을 수 있다. 또한, 스탠다드 1 (Standard 1) (머크 (Merck) 제품) 또는 BHI (Brain Heart Infusion, 디프코 제품) 등과 같이 제조 회사가 시판하는 것을 구입할 수도 있다.

모든 배지 성분들을 열처리 (1.5 bar 및 121℃에서 20분) 또는 멸균 여과로 멸균시킨다. 이 성분들은 한꺼번에 멸균시킬 수도 있고 필요에 따라서는 따로 멸균시킬 수도 있다. 모든 배지 성분들은 배양 초기부터 함유시킬 수도 있고, 원한다면 연속적으로 또는 배치식으로 (batchwise) 첨가할 수도 있다.

배양 조건은 각 실험마다 따로 정의된다. 온도는 15℃와 45℃ 사이의 범위이어야 하고, 실험하는 동안 일정하게 유지시키거나 변경시킬 수 있다. 배지의 pH는 5 내지 8.5의 범위, 바람직하게는 약 7.0 정도이어야 하며, 배지에 완충액을 첨가하여 유지시킬 수 있다. 이러한 목적으로 사용할 수 있는 완충액의 예로는 인산칼륨 완충액 등이 있다. MOPS, HEPES, ACES 등과 같은 합성 완충액을 대안적으로 사용하거나 동시에 사용할 수 있다. 또한, 배양하는 동안 NaOH 또는 NH₄OH를 첨가하여 pH를 일정하게 유지시킬 수도 있다. 효모 추출물 등의 복합 배지 성분이 사용되는 경우, 많은 복합 화합물들은 완충 성능이 높기 때문에 추가 완충액에 대한 요구를 감소시킬 수 있다. 미생물 배양에 발효기를 사용하는 경우, pH는 암모니아 기체를 사용하여 조절할 수도 있다.

인큐베이션 기간은 통상적으로 수 시간 내지 수 일이다. 이 시간은 브로쓰 (broth)에 축적되는 생성물의 양이 최대가 되도록 선택한다. 개시한 성장 실험을 다양한 크기의 미량역가 플레이트, 유리관, 유리 플라스크 또는 유리 또는 금속 발효기 등의 각종 용기에서 수행할 수 있다. 다수의 클론을 스크리닝하기 위해서는, 미생물을 배플 (baffle)이 있거나 없는 미량역가 플레이트, 유리관 또는 진탕 플라 스크에서 배양해야 한다. 요구되는 성장 배지를 100 mL 진탕 플라스크에 10％ (부피 기준) 채워 사용하는 것이 바람직하다. 이 플라스크들은 100 내지 300 rpm 속력 범위의 회전 진탕기 상에서 진탕 (진폭 25 mm)되어야 한다. 대기를 습하게 유지함으로써 증발로 인한 손실량을 감소시키거나, 별법으로는 증발로 인한 손실량을 정확하게 보정해야 한다.

유전자 변형된 클론을 연구하는 경우, 변형되지 않은 대조 클론 또는 인서트가 없는 원래의 플라스미드를 함유하는 대조 클론도 분석해야 한다. CM 플레이트 (10 g/L 글루코스, 2.5 g/L NaCl, 2 g/L 우레아, 10 g/L 폴리펩톤, 5 g/L 효모 추출물, 5 g/L 육류 추출물, 22 g/L 아가, 2 M NaOH을 사용하여 pH 6.8로 조정) 등과 같은 아가 플레이트 상에서 30℃에서 인큐베이션하여 성장시킨 세포를 OD₆₀₀이 0.5 내지 1.5가 되도록 배지에 접종한다. 배지 접종은 CM 플레이트로부터의 코리네박테리움 글루타미쿰 세포의 염수 용액을 도입하거나, 상기 박테리아의 예비배양액을 첨가하여 수행한다.

실시예 8: 돌연변이된 단백질의 기능에 관한 시험관내 분석

효소의 활성 및 반응 속도 (kinetics) 측정법은 당업계에 공지되어 있다. 특정하게 변형된 효소의 활성 측정 실험은 야생형 효소의 비활성(比活性)에 맞추어야 하며, 당업자라면 이를 잘 수행할 수 있다. 효소에 관한 일반적인 개요 뿐만 아니라 효소의 구조, 반응 속도, 원리, 방법, 적용에 관한 구체적인 세부사항 및 많은 효소들의 활성 측정 방법의 예는 예를 들어 하기의 참고문헌에서 찾을 수 있 다: [Dixon, M., and Webb, E.C., (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism. Freeman: New York], [Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: SanFrancisco], [Price, N.C., Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ. Press: Oxford], [Boyer, P.D., ed. (1983) The Enzymes, 3rd edition. Academic Press: New York], [Bisswanger, H., (1994) Enzymkinetik, 2nd edition. VCH: Weinheim (ISBN 3527300325)], [Bergmeyer, H.U., Bergmeyer, J., Grassl, M., eds. (1983-1986) Methods of Enzymatic Analysis, 3rd edition, vol.I-XII, Verlag Chemie: Weinheim] 및 [Ullmann's Encyclopedia of Industrial Chemistry (1987) vol.A9, "Enzymes". VCH: Weinheim, p.352-363].

DNA에 결합하는 단백질의 활성은 DNA 밴드-변위 (bandshift) 분석법 (겔 지연 분석법 (gel retardation assays)이라고 지칭하기도 함) 등과 같이 잘 수립되어 있는 여러 방법들을 사용하여 측정할 수 있다. 상기 단백질들이 다른 분자들의 발현에 미치는 영향은 리포터 유전자 분석법 (예를 들어 문헌 [Kolmar, H. et al. (1995) EMBO J. 14:3895-3904]과 그의 참고문헌에 기재된 바와 같음)을 사용하여 측정할 수 있다. β-갈락토시다제 등의 효소, 녹색 형광 단백질 및 여러가지 기타 효소가 사용되는 리포터 유전자 시험 시스템은 원핵 세포 및 진핵 세포 모두에의 적용에 대해 공지되어 있으며 잘 수립되어 있다.

막 수송 단백질의 활성은 문헌 [Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p.85-137, 199-234 및 270-322]에 기재된 기술에 따라 측정할 수 있다.

실시예 9: 돌연변이된 단백질이 관심 생성물의 생산에 미치는 영향 분석

코리네박테리움 글루타미쿰에서의 유전자 변형이 관심 화합물 (예컨대 아미노산)의 생산에 미치는 영향은 변형된 미생물을 적합한 조건 (예를 들어 상기 기재한 조건)하에서 성장시키고, 관심 생성물 (즉, 아미노산)의 생산 증가에 관여한 배지 성분 및(또는) 세포내 성분을 시험하여 결정할 수 있다. 이러한 분석 기술은 당업자에게 공지되어 있으며, 분광분석법, 박층 크로마토그래피법, 각종 유형의 착색법, 효소적 및 미생물학적 방법 및 고성능 액체 크로마토그래피법 등과 같은 분석용 크로마토그래피법 등이 있다 (예를 들어 문헌 ([Ullman, Encyclopedia of Industrial Chemistry, vol.A2, p.89-90 및 p.443-613, VCH: Weinheim (1985)], [Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and Molecular Biology, vol.17], [Rehm et al. (1993) Biotechnology, vol.3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim], [Belter, P.A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons], [Kennedy, J.F. and Cabral, J.M.S. (1992) Recovery processes for biological materials, John Wiley and Sons], [Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in Ulmann's Encyclopedia of Industrial Chemistry, vol.B3, Chapter 11, page 1-27, VCH: Weinheim] 및 [Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications]) 참조).

최종 발효 생성물의 측정 뿐만 아니라, 중간체 및 부산물 등과 같이 관심 화합물의 생산에 이용된 대사 경로 중의 다른 성분들을 분석함으로써 전반적인 유기체의 생산량, 화합물의 수율 및(또는) 생산 효율을 측정할 수도 있다. 분석 방법으로는 배지 내의 영양분 (예컨대 당, 탄화수소, 질소 공급원, 인산염 및 기타 이온) 함량 측정, 생물집단 (biomass)의 조성 및 성장 측정, 생합성 경로의 공통 대사물질의 생산 분석 및 발효 동안에 생성된 기체의 측정 등이 있다. 이러한 측정을 위한 표준 방법은 문헌 [Applied Microbial Physiology, A Practical Approach, P.M.Rhodes and P.F.Stanbury, eds., IRL Press, p.103-129, 131-163 및 165-192 (ISBN:0199635773)과 그의 참고문헌]에 기재되어 있다.

실시예 10: 코리네박테리움 글루타미쿰 배양물로부터의 관심 생성물 정제

당업계에 공지된 여러가지 방법을 사용하여, 코리네박테리움 글루타미쿰 세포 또는 상기 기재한 배양물의 상층액으로부터 관심 생성물을 수득할 수 있다. 세포가 관심 생성물을 분비하지 않는 경우에는 배양물을 저속 원심분리하여 세포를 수거하고 기계적인 힘이나 초음파 등과 같은 표준 기술로 세포를 용균시킬 수 있다. 원심분리로 세포 잔해를 제거하여 가용성 단백질을 함유하는 상층액 분획물을 수득하고, 이를 사용하여 관심 화합물을 추가로 정제한다. 코리네박테리움 글루타미쿰 세포가 생성물을 분비하는 경우에는 배양물을 저속 원심분리하여 세포를 제거하고, 수득한 상층액 분획물을 추가로 정제한다.

상기 두 가지 정제 방법 모두의 경우에서 수득한 상층액 분획물을 적합한 수지를 사용한 크로마토그래피에 적용하여 관심 분자는 크로마토그래피 수지 상에 남지만 샘플 중의 많은 오염물질들은 남지 않게 하거나, 오염물질들은 수지에 남지만 원하는 분자는 남지 않게 한다. 필요에 따라서는, 동일하거나 상이한 크로마토그래피 수지를 사용하여 이러한 크로마토그래피 단계를 반복할 수 있다. 당업자라면, 적합한 크로마토그래피 수지를 선택하고 정제할 특정 분자에 대해 가장 효과적으로 이를 적용하는 것에 숙달되어 있을 것이다. 정제된 생성물을 여과 또는 한외여과사켜 농축시키고, 생성물의 안정성이 최대가 되는 온도에 저장할 수 있다.

당업계에는 상기 기재한 정제법 및 예를 들어 문헌 [Bailey, J.E. ＆ Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986)]에 기재된 정제법 등을 포함하나 이에 제한되지 않는 여러가지 정제법들이 공지되어 있다.

단리된 화합물의 확인 및 순도는 당업계의 기술로 측정할 수 있다. 이러한 기술로는 고성능 액체 크로마토그래피법 (HPLC), 분광분석법, 착색법, 박층 크로마토그래피법, NIRS법, 효소 분석법 또는 미생물학적 분석법 등이 있다. 이러한 분석 방법은 하기 문헌에서 고찰된다: [Patek et al. (1994) Appl. Environ. Microbiol. 60:133-140], [Malakhova et al. (1996) Biotekhnologiya 11:27-32], [Schmidt et al. (1998) Bioprocess Engineer. 19:67-70], [Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol.A27, VCH: Weinheim, p.89-90, p.521-540, p.540-547, p.559-566, p.575-581 및 p.581-587], [Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons] 및 [Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol.17].

등가물

당업자라면, 단순한 통상의 방법을 이용하여 본 발명의 특정 실시양태의 수많은 등가물을 알아내거나 확인할 수 있다. 이러한 등가물은 하기하는 청구의 범위에 포함된다.

표 1의 정보는 다음과 같이 이해되어야 한다:

컬럼 1에서, "DNA 서열 번호"의 해당 번호는 첨부된 서열 목록에서의 서열 번호 각각을 지칭하는 것이다. 따라서, 컬럼 "DNA 서열 번호"의 "5"는 서열 5를 지칭한다.

컬럼 2에서, "아미노산 서열 번호"의 해당 번호는 첨부된 서열 목록에서의 서열 번호 각각을 지칭하는 것이다. 따라서, 컬럼 "아미노산 서열 번호"의 "6"은 서열 6을 지칭한다.

컬럼 3에서, "확인"은 각 서열에 대한 명확한 식별기호를 기재한 것이다.

컬럼 4에서, "아미노산 위치"의 해당 번호는 각 경우에서 동일 열에 있는 "아미노산 서열 번호"에 기재된 폴리펩티드 서열에서의 아미노산 위치를 지칭한다. 따라서, 컬럼 "아미노산 위치"의 "26"은 해당 폴리펩티드 서열의 아미노산 위치 26이다. 위치 번호는 N 말단을 +1로 하여 출발한다.

컬럼 5에서, "아미노산 야생형"의 해당 문자는 각 경우의 컬럼 4에 표시한 야생형 서열에서 해당 위치에 존재하는 아미노산을 지칭하며, 1문자 코드로 나타내었다.

컬럼 6에서, "아미노산 돌연변이체"의 해당 문자는 각 경우의 컬럼 4에 표시한 돌연변이체 서열에서 해당 위치에 존재하는 아미노산을 지칭하며, 1문자 코드로 나타내었다.

컬럼 7에서, "기능"은 해당 폴리펩티드 서열의 생리적 기능을 기재한 것이다.

단백질성 아미노산의 1문자 코드는 다음과 같다:

A 알라닌

C 시스테인

D 아스파르트산

E 글루탐산

F 페닐알라닌

G 글리신

H 히스티딘

I 이소루이신

K 리신

L 루이신

M 메티오닌

N 아스파라긴

P 프롤린

Q 글루타민

R 아르기닌

S 세린

T 트레오닌

V 발린

W 트립토판

Y 티로신

SEQUENCE LISTING <110> BASF Aktiengesellschaft <120> Genes coding for metabolic pathway proteins <130> O.Z. 0050/52968 <160> 164 <210> 1 <211> 1339 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1309) <223> RXA00116 <400> 1 gcaccctctc tttttttgtg tttgtggggg tcttgggccc cttgtgcacg cggcacgcac 60 gctgggggca agcgtcgaca agcacaaact ttttgcttaa ttg aat cct ttg cgc 115 Leu Asn Pro Leu Arg 1 5 acc aat caa tgg ggg atc aaa tat agt agc tgc atg agt aat gac ttc 163 Thr Asn Gln Trp Gly Ile Lys Tyr Ser Ser Cys Met Ser Asn Asp Phe 10 15 20 gtc gtt tct agg ctt aga ccc ttt ggt gaa acg att ttt gca acc atg 211 Val Val Ser Arg Leu Arg Pro Phe Gly Glu Thr Ile Phe Ala Thr Met 25 30 35 acc cag cga gct gtt gag gcg ggt gca atc aat ctt ggt cag ggc ttt 259 Thr Gln Arg Ala Val Glu Ala Gly Ala Ile Asn Leu Gly Gln Gly Phe 40 45 50 cct gat gag gat ggt cct cgt cgg atg tta gag atc gcg tcg gag cag 307 Pro Asp Glu Asp Gly Pro Arg Arg Met Leu Glu Ile Ala Ser Glu Gln 55 60 65 att ctc ggg gga aat aat cag tat tcg gcg ggg cgt ggg gat gct tcg 355 Ile Leu Gly Gly Asn Asn Gln Tyr Ser Ala Gly Arg Gly Asp Ala Ser 70 75 80 85 ttg agg gca gct gtg gct cgt gat cat ttg gag agg ttt gat ctg gag 403 Leu Arg Ala Ala Val Ala Arg Asp His Leu Glu Arg Phe Asp Leu Glu 90 95 100 tac aac cct gat tcg gag gtg ttg atc acg gtg ggg gcc act gag gcg 451 Tyr Asn Pro Asp Ser Glu Val Leu Ile Thr Val Gly Ala Thr Glu Ala 105 110 115 att acg gcg act gtg ttg ggt ttg gtg gag cct ggg gat gaa gtg atc 499 Ile Thr Ala Thr Val Leu Gly Leu Val Glu Pro Gly Asp Glu Val Ile 120 125 130 gtt ttg gaa ccg tat tac gat gcg tat gcg gcg gct att gcg ttg gcg 547 Val Leu Glu Pro Tyr Tyr Asp Ala Tyr Ala Ala Ala Ile Ala Leu Ala 135 140 145 ggg gcg acg cgg gtg gcg gtt cct ttg cag gag gtg gag aac tcg tgg 595 Gly Ala Thr Arg Val Ala Val Pro Leu Gln Glu Val Glu Asn Ser Trp 150 155 160 165 gat gtg gat gtc gat aag ttg cat gcg gcg gtg act aag aag acg cgg 643 Asp Val Asp Val Asp Lys Leu His Ala Ala Val Thr Lys Lys Thr Arg 170 175 180 atg att atc gtt aat tcg ccg cat aat ccg acg ggt tcg gtg ttt tct 691 Met Ile Ile Val Asn Ser Pro His Asn Pro Thr Gly Ser Val Phe Ser 185 190 195 aag aag gcg ttg aag cag ttg gcg ggt gtt gct cgt gcg tat gac ttg 739 Lys Lys Ala Leu Lys Gln Leu Ala Gly Val Ala Arg Ala Tyr Asp Leu 200 205 210 ttg gtg ttg tca gat gag gtg tat gag cat ctt gtt ttt gat gat cag 787 Leu Val Leu Ser Asp Glu Val Tyr Glu His Leu Val Phe Asp Asp Gln 215 220 225 aag cat gtg agt gtc gcg aag ctg ccc ggt atg tgg gat cgc acg gtg 835 Lys His Val Ser Val Ala Lys Leu Pro Gly Met Trp Asp Arg Thr Val 230 235 240 245 acg gtg tcg tcg gcg gcg aaa acg ttc aat gtg act ggt tgg aag acg 883 Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val Thr Gly Trp Lys Thr 250 255 260 ggg tgg gcg ttg gca ccg gag ccg ttg ttg gag gcg gtg ttg aag gcg 931 Gly Trp Ala Leu Ala Pro Glu Pro Leu Leu Glu Ala Val Leu Lys Ala 265 270 275 aag cag ttt atg tct tat gtg ggg gct aca cct ttt cag ccg gct gtg 979 Lys Gln Phe Met Ser Tyr Val Gly Ala Thr Pro Phe Gln Pro Ala Val 280 285 290 gcg cat gcg att gaa cat gag cag aag tgg gtg tca aag atg tct aag 1027 Ala His Ala Ile Glu His Glu Gln Lys Trp Val Ser Lys Met Ser Lys 295 300 305 ggg ctt gag ctc aag cgg gat att ttg cgt act gcg tta gat aag gcg 1075 Gly Leu Glu Leu Lys Arg Asp Ile Leu Arg Thr Ala Leu Asp Lys Ala 310 315 320 325 ggg ctg aag act cat gac agt atg ggc acg tat ttc atc gtt gcg gat 1123 Gly Leu Lys Thr His Asp Ser Met Gly Thr Tyr Phe Ile Val Ala Asp 330 335 340 att ggg gat cgt gat ggt gcg gag ttc tgt ttt gag ttg att gag aag 1171 Ile Gly Asp Arg Asp Gly Ala Glu Phe Cys Phe Glu Leu Ile Glu Lys 345 350 355 gtt ggg gtg gcg gcg att ccg gtg cag gcg ttt gtg gat cat ccg aag 1219 Val Gly Val Ala Ala Ile Pro Val Gln Ala Phe Val Asp His Pro Lys 360 365 370 aag tgg tcg tcg aag gtt cgt ttt gcg ttt tgc aaa aaa gaa gag acg 1267 Lys Trp Ser Ser Lys Val Arg Phe Ala Phe Cys Lys Lys Glu Glu Thr 375 380 385 ctc cgc gaa gct gcg gag cgt ctc aag ggg att aag aaa cta 1309 Leu Arg Glu Ala Ala Glu Arg Leu Lys Gly Ile Lys Lys Leu 390 395 400 tagtttgaac aggttgttgg gggtcttttt 1339 <210> 2 <211> 403 <212> PRT <213> Corynebacterium glutamicum <400> 2 Leu Asn Pro Leu Arg Thr Asn Gln Trp Gly Ile Lys Tyr Ser Ser Cys 1 5 10 15 Met Ser Asn Asp Phe Val Val Ser Arg Leu Arg Pro Phe Gly Glu Thr 20 25 30 Ile Phe Ala Thr Met Thr Gln Arg Ala Val Glu Ala Gly Ala Ile Asn 35 40 45 Leu Gly Gln Gly Phe Pro Asp Glu Asp Gly Pro Arg Arg Met Leu Glu 50 55 60 Ile Ala Ser Glu Gln Ile Leu Gly Gly Asn Asn Gln Tyr Ser Ala Gly 65 70 75 80 Arg Gly Asp Ala Ser Leu Arg Ala Ala Val Ala Arg Asp His Leu Glu 85 90 95 Arg Phe Asp Leu Glu Tyr Asn Pro Asp Ser Glu Val Leu Ile Thr Val 100 105 110 Gly Ala Thr Glu Ala Ile Thr Ala Thr Val Leu Gly Leu Val Glu Pro 115 120 125 Gly Asp Glu Val Ile Val Leu Glu Pro Tyr Tyr Asp Ala Tyr Ala Ala 130 135 140 Ala Ile Ala Leu Ala Gly Ala Thr Arg Val Ala Val Pro Leu Gln Glu 145 150 155 160 Val Glu Asn Ser Trp Asp Val Asp Val Asp Lys Leu His Ala Ala Val 165 170 175 Thr Lys Lys Thr Arg Met Ile Ile Val Asn Ser Pro His Asn Pro Thr 180 185 190 Gly Ser Val Phe Ser Lys Lys Ala Leu Lys Gln Leu Ala Gly Val Ala 195 200 205 Arg Ala Tyr Asp Leu Leu Val Leu Ser Asp Glu Val Tyr Glu His Leu 210 215 220 Val Phe Asp Asp Gln Lys His Val Ser Val Ala Lys Leu Pro Gly Met 225 230 235 240 Trp Asp Arg Thr Val Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val 245 250 255 Thr Gly Trp Lys Thr Gly Trp Ala Leu Ala Pro Glu Pro Leu Leu Glu 260 265 270 Ala Val Leu Lys Ala Lys Gln Phe Met Ser Tyr Val Gly Ala Thr Pro 275 280 285 Phe Gln Pro Ala Val Ala His Ala Ile Glu His Glu Gln Lys Trp Val 290 295 300 Ser Lys Met Ser Lys Gly Leu Glu Leu Lys Arg Asp Ile Leu Arg Thr 305 310 315 320 Ala Leu Asp Lys Ala Gly Leu Lys Thr His Asp Ser Met Gly Thr Tyr 325 330 335 Phe Ile Val Ala Asp Ile Gly Asp Arg Asp Gly Ala Glu Phe Cys Phe 340 345 350 Glu Leu Ile Glu Lys Val Gly Val Ala Ala Ile Pro Val Gln Ala Phe 355 360 365 Val Asp His Pro Lys Lys Trp Ser Ser Lys Val Arg Phe Ala Phe Cys 370 375 380 Lys Lys Glu Glu Thr Leu Arg Glu Ala Ala Glu Arg Leu Lys Gly Ile 385 390 395 400 Lys Lys Leu <210> 3 <211> 1309 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1279) <223> RXA00147 <400> 3 attgcatata atgcaatgaa ttgaataaac tacattcagg gttatcaacc agccaatttc 60 ttttaaaaag gcagacacac gaaaggcgac aacagtcacc gtg agt aaa gac acc 115 Val Ser Lys Asp Thr 1 5 acc acc tac cag gga gtc acc gag atc gga tcc gtt ccg gca tac ctg 163 Thr Thr Tyr Gln Gly Val Thr Glu Ile Gly Ser Val Pro Ala Tyr Leu 10 15 20 gtt ctt gca gac gga cgt acc ttc acc gga ttt ggc ttt gga gct atc 211 Val Leu Ala Asp Gly Arg Thr Phe Thr Gly Phe Gly Phe Gly Ala Ile 25 30 35 ggc acc acc ctt ggt gag gca gtg ttc act acc gcc atg acc ggt tac 259 Gly Thr Thr Leu Gly Glu Ala Val Phe Thr Thr Ala Met Thr Gly Tyr 40 45 50 caa gaa acc atg acc gat cct tcc tat cac cgc cag att gtt gtg gct 307 Gln Glu Thr Met Thr Asp Pro Ser Tyr His Arg Gln Ile Val Val Ala 55 60 65 acc gca cca cag atc ggc aac acc ggc tgg aac gat gag gac aac gag 355 Thr Ala Pro Gln Ile Gly Asn Thr Gly Trp Asn Asp Glu Asp Asn Glu 70 75 80 85 tcc cgc gac ggc aag att tgg gtt gca ggc ctt gtt atc cgc gac ctc 403 Ser Arg Asp Gly Lys Ile Trp Val Ala Gly Leu Val Ile Arg Asp Leu 90 95 100 gca gca cgt gtg tcc aac tgg cgc gcc acc acc tcc ttg cag cag gaa 451 Ala Ala Arg Val Ser Asn Trp Arg Ala Thr Thr Ser Leu Gln Gln Glu 105 110 115 atg gca ggc cag ggc atc gtc ggc atc ggc gga atc gac acc cgc gca 499 Met Ala Gly Gln Gly Ile Val Gly Ile Gly Gly Ile Asp Thr Arg Ala 120 125 130 ctg gtt cgc cac ctg cgc aat gaa ggt tcc att gca gcg ggc atc ttc 547 Leu Val Arg His Leu Arg Asn Glu Gly Ser Ile Ala Ala Gly Ile Phe 135 140 145 tcc ggc gct gac gca cag cgc cca gtt gaa gaa ctc gta gag atc gtc 595 Ser Gly Ala Asp Ala Gln Arg Pro Val Glu Glu Leu Val Glu Ile Val 150 155 160 165 aag aat cag cca gca atg acc ggc gca aac ctc tcc gtt gag gtc tct 643 Lys Asn Gln Pro Ala Met Thr Gly Ala Asn Leu Ser Val Glu Val Ser 170 175 180 gct gat gaa acc tac gtc atc gaa gct gaa ggc gaa gag cgc cac acc 691 Ala Asp Glu Thr Tyr Val Ile Glu Ala Glu Gly Glu Glu Arg His Thr 185 190 195 gtc gtg gcc tac gac ctg ggc att aag caa aac acc cca cgt cgt ttc 739 Val Val Ala Tyr Asp Leu Gly Ile Lys Gln Asn Thr Pro Arg Arg Phe 200 205 210 tct gca cgc ggt gtt cgc acc gtc atc gtg cct gct gaa acc cca ttc 787 Ser Ala Arg Gly Val Arg Thr Val Ile Val Pro Ala Glu Thr Pro Phe 215 220 225 gag gat atc aag cag tac aac cca tca ggc gtg ttc atc tcc aac ggc 835 Glu Asp Ile Lys Gln Tyr Asn Pro Ser Gly Val Phe Ile Ser Asn Gly 230 235 240 245 cct ggc gat cct gca gca gca gac gtc atg gtt gat atc gtc cgc gaa 883 Pro Gly Asp Pro Ala Ala Ala Asp Val Met Val Asp Ile Val Arg Glu 250 255 260 gtt ctt gaa gcc gac att cca ttc ttt ggc atc tgc ttc ggc aac cag 931 Val Leu Glu Ala Asp Ile Pro Phe Phe Gly Ile Cys Phe Gly Asn Gln 265 270 275 att ctt ggc cgc gca ttc ggc atg gag acc tac aag ctg aag ttc ggc 979 Ile Leu Gly Arg Ala Phe Gly Met Glu Thr Tyr Lys Leu Lys Phe Gly 280 285 290 cac cgc ggc atc aac gtt cca gtg aag aac cac atc acc ggc aag atc 1027 His Arg Gly Ile Asn Val Pro Val Lys Asn His Ile Thr Gly Lys Ile 295 300 305 gac atc acc gcc cag aac cac ggc ttc gca ctc aag ggt gaa gca ggc 1075 Asp Ile Thr Ala Gln Asn His Gly Phe Ala Leu Lys Gly Glu Ala Gly 310 315 320 325 cag gaa ttc gag acc gat ttc ggc act gca att gtc acc cac acc tgc 1123 Gln Glu Phe Glu Thr Asp Phe Gly Thr Ala Ile Val Thr His Thr Cys 330 335 340 ctc aac gac ggc gtc gtt gaa ggt att gcg ctg aag tcc gga cgc gca 1171 Leu Asn Asp Gly Val Val Glu Gly Ile Ala Leu Lys Ser Gly Arg Ala 345 350 355 tac tcc gtt cag tac cac cca gag gcc gct gcc ggc cca aat gat gca 1219 Tyr Ser Val Gln Tyr His Pro Glu Ala Ala Ala Gly Pro Asn Asp Ala 360 365 370 agc ccc ctg ttt gac cag ttt gtt gag ctg atg gat gca gac gct cag 1267 Ser Pro Leu Phe Asp Gln Phe Val Glu Leu Met Asp Ala Asp Ala Gln 375 380 385 aag aaa ggc gca taaataacat gccaaagcgt tcagatatta 1309 Lys Lys Gly Ala 390 <210> 4 <211> 393 <212> PRT <213> Corynebacterium glutamicum <400> 4 Val Ser Lys Asp Thr Thr Thr Tyr Gln Gly Val Thr Glu Ile Gly Ser 1 5 10 15 Val Pro Ala Tyr Leu Val Leu Ala Asp Gly Arg Thr Phe Thr Gly Phe 20 25 30 Gly Phe Gly Ala Ile Gly Thr Thr Leu Gly Glu Ala Val Phe Thr Thr 35 40 45 Ala Met Thr Gly Tyr Gln Glu Thr Met Thr Asp Pro Ser Tyr His Arg 50 55 60 Gln Ile Val Val Ala Thr Ala Pro Gln Ile Gly Asn Thr Gly Trp Asn 65 70 75 80 Asp Glu Asp Asn Glu Ser Arg Asp Gly Lys Ile Trp Val Ala Gly Leu 85 90 95 Val Ile Arg Asp Leu Ala Ala Arg Val Ser Asn Trp Arg Ala Thr Thr 100 105 110 Ser Leu Gln Gln Glu Met Ala Gly Gln Gly Ile Val Gly Ile Gly Gly 115 120 125 Ile Asp Thr Arg Ala Leu Val Arg His Leu Arg Asn Glu Gly Ser Ile 130 135 140 Ala Ala Gly Ile Phe Ser Gly Ala Asp Ala Gln Arg Pro Val Glu Glu 145 150 155 160 Leu Val Glu Ile Val Lys Asn Gln Pro Ala Met Thr Gly Ala Asn Leu 165 170 175 Ser Val Glu Val Ser Ala Asp Glu Thr Tyr Val Ile Glu Ala Glu Gly 180 185 190 Glu Glu Arg His Thr Val Val Ala Tyr Asp Leu Gly Ile Lys Gln Asn 195 200 205 Thr Pro Arg Arg Phe Ser Ala Arg Gly Val Arg Thr Val Ile Val Pro 210 215 220 Ala Glu Thr Pro Phe Glu Asp Ile Lys Gln Tyr Asn Pro Ser Gly Val 225 230 235 240 Phe Ile Ser Asn Gly Pro Gly Asp Pro Ala Ala Ala Asp Val Met Val 245 250 255 Asp Ile Val Arg Glu Val Leu Glu Ala Asp Ile Pro Phe Phe Gly Ile 260 265 270 Cys Phe Gly Asn Gln Ile Leu Gly Arg Ala Phe Gly Met Glu Thr Tyr 275 280 285 Lys Leu Lys Phe Gly His Arg Gly Ile Asn Val Pro Val Lys Asn His 290 295 300 Ile Thr Gly Lys Ile Asp Ile Thr Ala Gln Asn His Gly Phe Ala Leu 305 310 315 320 Lys Gly Glu Ala Gly Gln Glu Phe Glu Thr Asp Phe Gly Thr Ala Ile 325 330 335 Val Thr His Thr Cys Leu Asn Asp Gly Val Val Glu Gly Ile Ala Leu 340 345 350 Lys Ser Gly Arg Ala Tyr Ser Val Gln Tyr His Pro Glu Ala Ala Ala 355 360 365 Gly Pro Asn Asp Ala Ser Pro Leu Phe Asp Gln Phe Val Glu Leu Met 370 375 380 Asp Ala Asp Ala Gln Lys Lys Gly Ala 385 390 <210> 5 <211> 1426 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1396) <223> RXA00152 <400> 5 gtcattgata tccaaggcac gaccgcgatt gtatggaaag aagcctaaat ttttaacaat 60 caaatagtac tggccattcc caactaaaac tggagtaacg atg aca gga cta atc 115 Met Thr Gly Leu Ile 1 5 ctc gcc ata gtt ttc ctg gtc ttt gtc gcc gtc gtg gtg atc aag tcc 163 Leu Ala Ile Val Phe Leu Val Phe Val Ala Val Val Val Ile Lys Ser 10 15 20 ata gcc ctg att ccc cag ggt gaa gcc gcc gtc att gaa cgc ctt ggt 211 Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val Ile Glu Arg Leu Gly 25 30 35 agc tac acc cgc acc gtt tca ggt ggc ctg acc ctg ctg gtt cca ttc 259 Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr Leu Leu Val Pro Phe 40 45 50 gtg gac cga gta cgc gca agg atc gac acc cgt gag cgc gtg gtc tca 307 Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg Glu Arg Val Val Ser 55 60 65 ttc cca ccg cag gct gtt att acc caa gac aac ctg acc gtg gcc atc 355 Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn Leu Thr Val Ala Ile 70 75 80 85 gat atc gtg gtg acc ttc caa atc aac gaa cca gag cgc gcc atc tac 403 Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro Glu Arg Ala Ile Tyr 90 95 100 ggc gtg gac aac tac atc gtc ggt gtg gag cag att tct gta gca aca 451 Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln Ile Ser Val Ala Thr 105 110 115 ctt cga gac gtt gtc ggt ggc atg acc ctg gaa gaa acc ctc act tca 499 Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu Glu Thr Leu Thr Ser 120 125 130 cgt gac gtg atc aac cgc cgc ctc cgt ggc gag ctc gat gca gca acc 547 Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu Leu Asp Ala Ala Thr 135 140 145 acc aaa tgg ggc ctg cgc atc agc cgt gtg gaa cta aag gca att gat 595 Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu Leu Lys Ala Ile Asp 150 155 160 165 ccg cca cca tcc atc cag caa tcg atg gaa aag cag atg aag gca gac 643 Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys Gln Met Lys Ala Asp 170 175 180 cgt gaa aag cgc gcc acc att ttg acc gca gaa ggt cag cgc gaa gcc 691 Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu Gly Gln Arg Glu Ala 185 190 195 gac atc aaa act gcc gaa ggt gaa aag caa gcc aag atc ctc caa gct 739 Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala Lys Ile Leu Gln Ala 200 205 210 gag ggt gaa aag cac gca tcc atc ctg aac gca gaa gca gaa cgc caa 787 Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala Glu Ala Glu Arg Gln 215 220 225 gcg atg atc ctg cgc gcc gaa ggt gaa cgc gca gca cgc tac ctc cag 835 Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala Ala Arg Tyr Leu Gln 230 235 240 245 gcg cag ggt gaa gcc cga gca atc caa aag gtc aac gca gca atc aag 883 Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val Asn Ala Ala Ile Lys 250 255 260 tct gcc aag ttg acc cca gag gtt ctt gct tat caa tac ctc gaa aag 931 Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr Gln Tyr Leu Glu Lys 265 270 275 ctt cct aag atc gca gag ggc aac gcc tcc aag atg tgg gtc atc cca 979 Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys Met Trp Val Ile Pro 280 285 290 agc cag ttc tcc gat tct ctg gaa ggt ttt gcg aag cag ttc ggc gca 1027 Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala Lys Gln Phe Gly Ala 295 300 305 aag gat gca gaa ggt gtc ttc cgc tac gaa cca aac acc gtg gat gaa 1075 Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro Asn Thr Val Asp Glu 310 315 320 325 gaa acc cgc gac atc gca aac gcc gac aac gtg gaa gac tgg ttc tcc 1123 Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val Glu Asp Trp Phe Ser 330 335 340 acc gaa tca gac cct gaa atc gca gca gca gtc gcc gca gca aac gcc 1171 Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val Ala Ala Ala Asn Ala 345 350 355 gtg gcc aac aag cca gtc gat cca gaa ccc ggt gag atc ctt tcc aag 1219 Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly Glu Ile Leu Ser Lys 360 365 370 aag acc gca cga cgc gtt gaa cct gaa gca gta ttg gag gct ttg caa 1267 Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val Leu Glu Ala Leu Gln 375 380 385 aac gga acc act aca caa cct gag gtt gag gca gca cct cct acc gca 1315 Asn Gly Thr Thr Thr Gln Pro Glu Val Glu Ala Ala Pro Pro Thr Ala 390 395 400 405 aac ttc gcc caa gaa ttc cct gca cca cag gca aac cct gaa gat tac 1363 Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala Asn Pro Glu Asp Tyr 410 415 420 tcc gac caa cac cga gag aat cct tac gga aac taatcaggca taagaaaagg 1416 Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn 425 430 cgggaaacac 1426 <210> 6 <211> 432 <212> PRT <213> Corynebacterium glutamicum <400> 6 Met Thr Gly Leu Ile Leu Ala Ile Val Phe Leu Val Phe Val Ala Val 1 5 10 15 Val Val Ile Lys Ser Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val 20 25 30 Ile Glu Arg Leu Gly Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr 35 40 45 Leu Leu Val Pro Phe Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg 50 55 60 Glu Arg Val Val Ser Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn 65 70 75 80 Leu Thr Val Ala Ile Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro 85 90 95 Glu Arg Ala Ile Tyr Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln 100 105 110 Ile Ser Val Ala Thr Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu 115 120 125 Glu Thr Leu Thr Ser Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu 130 135 140 Leu Asp Ala Ala Thr Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu 145 150 155 160 Leu Lys Ala Ile Asp Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys 165 170 175 Gln Met Lys Ala Asp Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu 180 185 190 Gly Gln Arg Glu Ala Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala 195 200 205 Lys Ile Leu Gln Ala Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala 210 215 220 Glu Ala Glu Arg Gln Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala 225 230 235 240 Ala Arg Tyr Leu Gln Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val 245 250 255 Asn Ala Ala Ile Lys Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr 260 265 270 Gln Tyr Leu Glu Lys Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys 275 280 285 Met Trp Val Ile Pro Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala 290 295 300 Lys Gln Phe Gly Ala Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro 305 310 315 320 Asn Thr Val Asp Glu Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val 325 330 335 Glu Asp Trp Phe Ser Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val 340 345 350 Ala Ala Ala Asn Ala Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly 355 360 365 Glu Ile Leu Ser Lys Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val 370 375 380 Leu Glu Ala Leu Gln Asn Gly Thr Thr Thr Gln Pro Glu Val Glu Ala 385 390 395 400 Ala Pro Pro Thr Ala Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala 405 410 415 Asn Pro Glu Asp Tyr Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn 420 425 430 <210> 7 <211> 1048 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1018) <223> RXA00166 <400> 7 tgcgacaacg ccactttgag agtccataat ttggcatcca acgccggcat cttggacggt 60 cgcgcgccgg gcccggggag gtttttatac ctatctaaag gtg tgg att tcg ttt 115 Val Trp Ile Ser Phe 1 5 gtt gca caa gga gat act gta tct cat gag cac gtt aag cga tat atc 163 Val Ala Gln Gly Asp Thr Val Ser His Glu His Val Lys Arg Tyr Ile 10 15 20 gct agt gaa ggt ggt ggt agc ccg cta gga tta aca tct gat atg aat 211 Ala Ser Glu Gly Gly Gly Ser Pro Leu Gly Leu Thr Ser Asp Met Asn 25 30 35 gat cca gct cat ccc cca att cca cca gta tca aaa cga gac gct ccg 259 Asp Pro Ala His Pro Pro Ile Pro Pro Val Ser Lys Arg Asp Ala Pro 40 45 50 gcg ttt agc gat ctt caa cat cga gca acc agc gcc agc gct ttt acc 307 Ala Phe Ser Asp Leu Gln His Arg Ala Thr Ser Ala Ser Ala Phe Thr 55 60 65 caa ggc agc acg act tat cac gat gtc cga cct gga tat ccg gct gag 355 Gln Gly Ser Thr Thr Tyr His Asp Val Arg Pro Gly Tyr Pro Ala Glu 70 75 80 85 gcc gtg gag tta gcc cgt ggg ttt ggc cga gtc ctg gat gtc ggt gca 403 Ala Val Glu Leu Ala Arg Gly Phe Gly Arg Val Leu Asp Val Gly Ala 90 95 100 ggt acc gga aaa cta acc agt gag cta aca gct gat cag gtc cta gcc 451 Gly Thr Gly Lys Leu Thr Ser Glu Leu Thr Ala Asp Gln Val Leu Ala 105 110 115 ctt gat cca agc atg gac atg ttg cgg gtg ttt cgc tcc gcg ctt ccg 499 Leu Asp Pro Ser Met Asp Met Leu Arg Val Phe Arg Ser Ala Leu Pro 120 125 130 gcg gtt ccc tgc tgg caa gcg aca gca gaa cac aca gga ata cgt gac 547 Ala Val Pro Cys Trp Gln Ala Thr Ala Glu His Thr Gly Ile Arg Asp 135 140 145 aac gcg gtt gat ctg att acg tgc gca caa acg tgg cat tgg gtt gac 595 Asn Ala Val Asp Leu Ile Thr Cys Ala Gln Thr Trp His Trp Val Asp 150 155 160 165 gtg acg gct gcc tca gcg gaa ttt gat cgg gtg att gca cct gag ggt 643 Val Thr Ala Ala Ser Ala Glu Phe Asp Arg Val Ile Ala Pro Glu Gly 170 175 180 gca gtc ctg ctc gtg tgg aat aac ctg gac acc tcc atc gcg tgg gta 691 Ala Val Leu Leu Val Trp Asn Asn Leu Asp Thr Ser Ile Ala Trp Val 185 190 195 cac cga ctc agt cgc att atg cat gcc ggc gat gta ctc aag ccg gga 739 His Arg Leu Ser Arg Ile Met His Ala Gly Asp Val Leu Lys Pro Gly 200 205 210 ttc acc cca gaa acc gca gct ccc tgg ata att gat cga gaa att cgc 787 Phe Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile Asp Arg Glu Ile Arg 215 220 225 acc acg tgg aat cag cac ctc acc cct gaa gaa atc atc cag ctc gct 835 Thr Thr Trp Asn Gln His Leu Thr Pro Glu Glu Ile Ile Gln Leu Ala 230 235 240 245 cac acg agg tcc tac tgg tta aac gcg tca gag aaa atc aaa gag cgt 883 His Thr Arg Ser Tyr Trp Leu Asn Ala Ser Glu Lys Ile Lys Glu Arg 250 255 260 gtt gat cag aac ctt cag tgg tat ctc tac gag cat ttg ggt ttc agt 931 Val Asp Gln Asn Leu Gln Trp Tyr Leu Tyr Glu His Leu Gly Phe Ser 265 270 275 ccc gac aat cca gtg gaa ctt ccc tat cgc tgt gat gca ttt tta ctt 979 Pro Asp Asn Pro Val Glu Leu Pro Tyr Arg Cys Asp Ala Phe Leu Leu 280 285 290 tca cgt tcc ggt acc ctg gca ggc aga tct tcc aat ctt taggagccct 1028 Ser Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser Asn Leu 295 300 305 cgccatgtac ctgttgaatc 1048 <210> 8 <211> 306 <212> PRT <213> Corynebacterium glutamicum <400> 8 Val Trp Ile Ser Phe Val Ala Gln Gly Asp Thr Val Ser His Glu His 1 5 10 15 Val Lys Arg Tyr Ile Ala Ser Glu Gly Gly Gly Ser Pro Leu Gly Leu 20 25 30 Thr Ser Asp Met Asn Asp Pro Ala His Pro Pro Ile Pro Pro Val Ser 35 40 45 Lys Arg Asp Ala Pro Ala Phe Ser Asp Leu Gln His Arg Ala Thr Ser 50 55 60 Ala Ser Ala Phe Thr Gln Gly Ser Thr Thr Tyr His Asp Val Arg Pro 65 70 75 80 Gly Tyr Pro Ala Glu Ala Val Glu Leu Ala Arg Gly Phe Gly Arg Val 85 90 95 Leu Asp Val Gly Ala Gly Thr Gly Lys Leu Thr Ser Glu Leu Thr Ala 100 105 110 Asp Gln Val Leu Ala Leu Asp Pro Ser Met Asp Met Leu Arg Val Phe 115 120 125 Arg Ser Ala Leu Pro Ala Val Pro Cys Trp Gln Ala Thr Ala Glu His 130 135 140 Thr Gly Ile Arg Asp Asn Ala Val Asp Leu Ile Thr Cys Ala Gln Thr 145 150 155 160 Trp His Trp Val Asp Val Thr Ala Ala Ser Ala Glu Phe Asp Arg Val 165 170 175 Ile Ala Pro Glu Gly Ala Val Leu Leu Val Trp Asn Asn Leu Asp Thr 180 185 190 Ser Ile Ala Trp Val His Arg Leu Ser Arg Ile Met His Ala Gly Asp 195 200 205 Val Leu Lys Pro Gly Phe Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile 210 215 220 Asp Arg Glu Ile Arg Thr Thr Trp Asn Gln His Leu Thr Pro Glu Glu 225 230 235 240 Ile Ile Gln Leu Ala His Thr Arg Ser Tyr Trp Leu Asn Ala Ser Glu 245 250 255 Lys Ile Lys Glu Arg Val Asp Gln Asn Leu Gln Trp Tyr Leu Tyr Glu 260 265 270 His Leu Gly Phe Ser Pro Asp Asn Pro Val Glu Leu Pro Tyr Arg Cys 275 280 285 Asp Ala Phe Leu Leu Ser Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser 290 295 300 Asn Leu 305 <210> 9 <211> 1162 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1132) <223> RXA00278 <400> 9 ggccacggag ttggtgtccg tcatggtgat gattctgtct gtcgtttctg tgttggcttt 60 gtgggtcggc atccgccccc gtttgcagga gtacttataa atg cac gct ttt cga 115 Met His Ala Phe Arg 1 5 cgc ccc cct cca ctc acc acg cga gtc ggc gct gca ttg ctg gcc gca 163 Arg Pro Pro Pro Leu Thr Thr Arg Val Gly Ala Ala Leu Leu Ala Ala 10 15 20 acg ctg ctt gct tcc tgc act cca aca cct gtg gaa ccg gca gaa acc 211 Thr Leu Leu Ala Ser Cys Thr Pro Thr Pro Val Glu Pro Ala Glu Thr 25 30 35 ttg act gct ttg gat ccc gat gcc ggt cca cca ctg cca ccg gat tct 259 Leu Thr Ala Leu Asp Pro Asp Ala Gly Pro Pro Leu Pro Pro Asp Ser 40 45 50 tcg att gaa gct ccc ggt gaa aaa gag ccc att gtg gaa gta ata gag 307 Ser Ile Glu Ala Pro Gly Glu Lys Glu Pro Ile Val Glu Val Ile Glu 55 60 65 aat tgg cca ggt tct tta cgc ccg gat gat ctg acc cct gag gag cgg 355 Asn Trp Pro Gly Ser Leu Arg Pro Asp Asp Leu Thr Pro Glu Glu Arg 70 75 80 85 gta cct ggc atc gtc aac cgg ggt cgc atc att gtg ggt gtg gat caa 403 Val Pro Gly Ile Val Asn Arg Gly Arg Ile Ile Val Gly Val Asp Gln 90 95 100 tcg caa aac ttg ctc agt ttc cgt gat ccg gtg act ggt gag ctg cgc 451 Ser Gln Asn Leu Leu Ser Phe Arg Asp Pro Val Thr Gly Glu Leu Arg 105 110 115 ggt ttt gaa gtg gaa tta gcg agg gaa att tcc cgc gac att ttc ggt 499 Gly Phe Glu Val Glu Leu Ala Arg Glu Ile Ser Arg Asp Ile Phe Gly 120 125 130 gac ccc aat aag gtg gat ttc cga ttc gtc ggc tcg tcc gac cgt ctg 547 Asp Pro Asn Lys Val Asp Phe Arg Phe Val Gly Ser Ser Asp Arg Leu 135 140 145 cgt tcc ctt gac caa ggt gat gta gat att gtg att cgt tcc gtc acg 595 Arg Ser Leu Asp Gln Gly Asp Val Asp Ile Val Ile Arg Ser Val Thr 150 155 160 165 atc acc gac gaa cgc gcc aaa ttg gtg gaa ttt tcc aca ccg tac ctg 643 Ile Thr Asp Glu Arg Ala Lys Leu Val Glu Phe Ser Thr Pro Tyr Leu 170 175 180 cgc acc caa acc cgc atg ttg acc atg gaa tct tca gga atc acg tcc 691 Arg Thr Gln Thr Arg Met Leu Thr Met Glu Ser Ser Gly Ile Thr Ser 185 190 195 atc gca gat cta ccc ggc cac acc att tgt gtc acc gat ggc tcc act 739 Ile Ala Asp Leu Pro Gly His Thr Ile Cys Val Thr Asp Gly Ser Thr 200 205 210 tca ttg cag cga gcc cgc acc att gcg ccg gag gcc tca atc tta aaa 787 Ser Leu Gln Arg Ala Arg Thr Ile Ala Pro Glu Ala Ser Ile Leu Lys 215 220 225 act cgc aat tgg tcc gat tgt ctc atg gcg ttg cag cag cat cag gct 835 Thr Arg Asn Trp Ser Asp Cys Leu Met Ala Leu Gln Gln His Gln Ala 230 235 240 245 cag gtc att ttg ggc gat gat gtc att ttg tcc ggc atc gca gca cag 883 Gln Val Ile Leu Gly Asp Asp Val Ile Leu Ser Gly Ile Ala Ala Gln 250 255 260 gat ccc tac acc gag att ctt gat acc tcc ctc gat tcc cat tcc tat 931 Asp Pro Tyr Thr Glu Ile Leu Asp Thr Ser Leu Asp Ser His Ser Tyr 265 270 275 gga gtg gca gcg gca tcg acc act gct gaa aca gac tct tcg ggg ttg 979 Gly Val Ala Ala Ala Ser Thr Thr Ala Glu Thr Asp Ser Ser Gly Leu 280 285 290 att cgg cag gta aac tac aca att gaa cgg atc cgc aca gac cgc atg 1027 Ile Arg Gln Val Asn Tyr Thr Ile Glu Arg Ile Arg Thr Asp Arg Met 295 300 305 tgg tgg aca atg ttc gac gat tgg ttc gga cct tat ctc tgg tcc tac 1075 Trp Trp Thr Met Phe Asp Asp Trp Phe Gly Pro Tyr Leu Trp Ser Tyr 310 315 320 325 ggt cca cca cag ctg cag tac atg cca gag gaa gaa ggg aca gaa aac 1123 Gly Pro Pro Gln Leu Gln Tyr Met Pro Glu Glu Glu Gly Thr Glu Asn 330 335 340 gat gaa gga taatgaagat ttcgatccag attcaccagc 1162 Asp Glu Gly <210> 10 <211> 344 <212> PRT <213> Corynebacterium glutamicum <400> 10 Met His Ala Phe Arg Arg Pro Pro Pro Leu Thr Thr Arg Val Gly Ala 1 5 10 15 Ala Leu Leu Ala Ala Thr Leu Leu Ala Ser Cys Thr Pro Thr Pro Val 20 25 30 Glu Pro Ala Glu Thr Leu Thr Ala Leu Asp Pro Asp Ala Gly Pro Pro 35 40 45 Leu Pro Pro Asp Ser Ser Ile Glu Ala Pro Gly Glu Lys Glu Pro Ile 50 55 60 Val Glu Val Ile Glu Asn Trp Pro Gly Ser Leu Arg Pro Asp Asp Leu 65 70 75 80 Thr Pro Glu Glu Arg Val Pro Gly Ile Val Asn Arg Gly Arg Ile Ile 85 90 95 Val Gly Val Asp Gln Ser Gln Asn Leu Leu Ser Phe Arg Asp Pro Val 100 105 110 Thr Gly Glu Leu Arg Gly Phe Glu Val Glu Leu Ala Arg Glu Ile Ser 115 120 125 Arg Asp Ile Phe Gly Asp Pro Asn Lys Val Asp Phe Arg Phe Val Gly 130 135 140 Ser Ser Asp Arg Leu Arg Ser Leu Asp Gln Gly Asp Val Asp Ile Val 145 150 155 160 Ile Arg Ser Val Thr Ile Thr Asp Glu Arg Ala Lys Leu Val Glu Phe 165 170 175 Ser Thr Pro Tyr Leu Arg Thr Gln Thr Arg Met Leu Thr Met Glu Ser 180 185 190 Ser Gly Ile Thr Ser Ile Ala Asp Leu Pro Gly His Thr Ile Cys Val 195 200 205 Thr Asp Gly Ser Thr Ser Leu Gln Arg Ala Arg Thr Ile Ala Pro Glu 210 215 220 Ala Ser Ile Leu Lys Thr Arg Asn Trp Ser Asp Cys Leu Met Ala Leu 225 230 235 240 Gln Gln His Gln Ala Gln Val Ile Leu Gly Asp Asp Val Ile Leu Ser 245 250 255 Gly Ile Ala Ala Gln Asp Pro Tyr Thr Glu Ile Leu Asp Thr Ser Leu 260 265 270 Asp Ser His Ser Tyr Gly Val Ala Ala Ala Ser Thr Thr Ala Glu Thr 275 280 285 Asp Ser Ser Gly Leu Ile Arg Gln Val Asn Tyr Thr Ile Glu Arg Ile 290 295 300 Arg Thr Asp Arg Met Trp Trp Thr Met Phe Asp Asp Trp Phe Gly Pro 305 310 315 320 Tyr Leu Trp Ser Tyr Gly Pro Pro Gln Leu Gln Tyr Met Pro Glu Glu 325 330 335 Glu Gly Thr Glu Asn Asp Glu Gly 340 <210> 11 <211> 1558 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1528) <223> RXA00323 <400> 11 ttccttattc gtaggagtgt tctaatttcg gtgcggttct cagtgaacca cccaagctgg 60 acacctccca cccccgtgtc atcaaaaaac cgcgacatcc ttg agt aac tct gag 115 Leu Ser Asn Ser Glu 1 5 aaa aac tac ccc cga tgg gag tat aaa agt ggc aaa tgc gca gtc gat 163 Lys Asn Tyr Pro Arg Trp Glu Tyr Lys Ser Gly Lys Cys Ala Val Asp 10 15 20 gtc cca tcg ctg cgt aga tta gtt ttc atg aac agc gaa cag gaa ttt 211 Val Pro Ser Leu Arg Arg Leu Val Phe Met Asn Ser Glu Gln Glu Phe 25 30 35 gta ctc agc gcc att gaa gaa cgc gac att aag ttt gtg cgt cta tgg 259 Val Leu Ser Ala Ile Glu Glu Arg Asp Ile Lys Phe Val Arg Leu Trp 40 45 50 ttc act gac att ctt ggc cac ttg aag tca gtg gtt gtg gct cct gca 307 Phe Thr Asp Ile Leu Gly His Leu Lys Ser Val Val Val Ala Pro Ala 55 60 65 gaa cta gag tct gcg ttg gaa gaa ggc atc gga ttc gat ggc tca gcc 355 Glu Leu Glu Ser Ala Leu Glu Glu Gly Ile Gly Phe Asp Gly Ser Ala 70 75 80 85 att gag ggc tac gcg cgt atc tcg gaa gcg gac acc att gcc cgc cca 403 Ile Glu Gly Tyr Ala Arg Ile Ser Glu Ala Asp Thr Ile Ala Arg Pro 90 95 100 gat cca tcg aca ttc cag gtc ctc cca cta gaa gcg ggc atc tca aaa 451 Asp Pro Ser Thr Phe Gln Val Leu Pro Leu Glu Ala Gly Ile Ser Lys 105 110 115 ctg cag gca gca cgc ctg ttt tgc gat gtc acg atg cca gac gga cag 499 Leu Gln Ala Ala Arg Leu Phe Cys Asp Val Thr Met Pro Asp Gly Gln 120 125 130 cca tct ttt tct gac ccg cgc caa gtg ctg cgc agg cag gtc caa cta 547 Pro Ser Phe Ser Asp Pro Arg Gln Val Leu Arg Arg Gln Val Gln Leu 135 140 145 gct gca gat gaa ggc ttg acc tgc atg atc tca cca gag att gag ttc 595 Ala Ala Asp Glu Gly Leu Thr Cys Met Ile Ser Pro Glu Ile Glu Phe 150 155 160 165 tat ttg gtg caa agc ctt cgc acc aac gga ctg cca cct gtg ccc act 643 Tyr Leu Val Gln Ser Leu Arg Thr Asn Gly Leu Pro Pro Val Pro Thr 170 175 180 gac aac ggc gga tat ttc gac caa gcc aca ttc aat gag gcg ccg aat 691 Asp Asn Gly Gly Tyr Phe Asp Gln Ala Thr Phe Asn Glu Ala Pro Asn 185 190 195 ttc cgt cga aac gcg atg gta gcg ctg gag gaa ctc ggc atc cct gtc 739 Phe Arg Arg Asn Ala Met Val Ala Leu Glu Glu Leu Gly Ile Pro Val 200 205 210 gag ttc tcc cac cat gaa act gca cct ggc cag caa gaa atc gat tta 787 Glu Phe Ser His His Glu Thr Ala Pro Gly Gln Gln Glu Ile Asp Leu 215 220 225 cgc cat gcg gat gcg ctc acc atg gcc gac aac atc atg acc ttc cgc 835 Arg His Ala Asp Ala Leu Thr Met Ala Asp Asn Ile Met Thr Phe Arg 230 235 240 245 tac atc atg aaa cag gtg gca agg gac caa ggc gtc ggg gca tca ttt 883 Tyr Ile Met Lys Gln Val Ala Arg Asp Gln Gly Val Gly Ala Ser Phe 250 255 260 atg ccc aag cca ttc caa gaa cat gca ggc tcc gcc atg cac acg cac 931 Met Pro Lys Pro Phe Gln Glu His Ala Gly Ser Ala Met His Thr His 265 270 275 atg tcc tta ttt gag ggc gat acc aac gcg ttc cac gat cca gac gat 979 Met Ser Leu Phe Glu Gly Asp Thr Asn Ala Phe His Asp Pro Asp Asp 280 285 290 tct tac atg ctg tcc aaa acc gca aaa cag ttc atc gct gga atc ttg 1027 Ser Tyr Met Leu Ser Lys Thr Ala Lys Gln Phe Ile Ala Gly Ile Leu 295 300 305 cat cac gct cca gaa ttc acc gct gtg acc aac cag tgg gtc aat tcc 1075 His His Ala Pro Glu Phe Thr Ala Val Thr Asn Gln Trp Val Asn Ser 310 315 320 325 tac aaa cgc atc gtg tac gga aac gaa gct cca act gcg gca acc tgg 1123 Tyr Lys Arg Ile Val Tyr Gly Asn Glu Ala Pro Thr Ala Ala Thr Trp 330 335 340 ggt gta tct aat cgt tct gcg ctg gtt cgt gtt cct acc tac cgt ttg 1171 Gly Val Ser Asn Arg Ser Ala Leu Val Arg Val Pro Thr Tyr Arg Leu 345 350 355 aat aag gag gag tcg cgc cgg gtg gag gtg cgt ctt cct gat acc gct 1219 Asn Lys Glu Glu Ser Arg Arg Val Glu Val Arg Leu Pro Asp Thr Ala 360 365 370 tgt aac cca tat ttg gcg ttt tca gtg atg ctc ggc gct ggt ttg aaa 1267 Cys Asn Pro Tyr Leu Ala Phe Ser Val Met Leu Gly Ala Gly Leu Lys 375 380 385 ggc att aaa gaa ggt tat gag ctc gac gag cca gct gag gac gat atc 1315 Gly Ile Lys Glu Gly Tyr Glu Leu Asp Glu Pro Ala Glu Asp Asp Ile 390 395 400 405 tcc aac ttg agc ttc cgg gaa cgt cgc gcc atg ggc tac aac gat ctg 1363 Ser Asn Leu Ser Phe Arg Glu Arg Arg Ala Met Gly Tyr Asn Asp Leu 410 415 420 cca agc agc ctt gat cag gca ctg cgc caa atg gaa aag tca gag ctt 1411 Pro Ser Ser Leu Asp Gln Ala Leu Arg Gln Met Glu Lys Ser Glu Leu 425 430 435 gtt gct gac atc ctc ggt gag cac gtt ttt gag ttt ttc ttg cgc aat 1459 Val Ala Asp Ile Leu Gly Glu His Val Phe Glu Phe Phe Leu Arg Asn 440 445 450 aag tgg cgt gaa tgg cgt gac tac caa gag cag atc act ccg tgg gag 1507 Lys Trp Arg Glu Trp Arg Asp Tyr Gln Glu Gln Ile Thr Pro Trp Glu 455 460 465 ctc cga aac aat ctt gat tac tagacttttg cactccaatg gaaaccctac 1558 Leu Arg Asn Asn Leu Asp Tyr 470 475 <210> 12 <211> 476 <212> PRT <213> Corynebacterium glutamicum <400> 12 Leu Ser Asn Ser Glu Lys Asn Tyr Pro Arg Trp Glu Tyr Lys Ser Gly 1 5 10 15 Lys Cys Ala Val Asp Val Pro Ser Leu Arg Arg Leu Val Phe Met Asn 20 25 30 Ser Glu Gln Glu Phe Val Leu Ser Ala Ile Glu Glu Arg Asp Ile Lys 35 40 45 Phe Val Arg Leu Trp Phe Thr Asp Ile Leu Gly His Leu Lys Ser Val 50 55 60 Val Val Ala Pro Ala Glu Leu Glu Ser Ala Leu Glu Glu Gly Ile Gly 65 70 75 80 Phe Asp Gly Ser Ala Ile Glu Gly Tyr Ala Arg Ile Ser Glu Ala Asp 85 90 95 Thr Ile Ala Arg Pro Asp Pro Ser Thr Phe Gln Val Leu Pro Leu Glu 100 105 110 Ala Gly Ile Ser Lys Leu Gln Ala Ala Arg Leu Phe Cys Asp Val Thr 115 120 125 Met Pro Asp Gly Gln Pro Ser Phe Ser Asp Pro Arg Gln Val Leu Arg 130 135 140 Arg Gln Val Gln Leu Ala Ala Asp Glu Gly Leu Thr Cys Met Ile Ser 145 150 155 160 Pro Glu Ile Glu Phe Tyr Leu Val Gln Ser Leu Arg Thr Asn Gly Leu 165 170 175 Pro Pro Val Pro Thr Asp Asn Gly Gly Tyr Phe Asp Gln Ala Thr Phe 180 185 190 Asn Glu Ala Pro Asn Phe Arg Arg Asn Ala Met Val Ala Leu Glu Glu 195 200 205 Leu Gly Ile Pro Val Glu Phe Ser His His Glu Thr Ala Pro Gly Gln 210 215 220 Gln Glu Ile Asp Leu Arg His Ala Asp Ala Leu Thr Met Ala Asp Asn 225 230 235 240 Ile Met Thr Phe Arg Tyr Ile Met Lys Gln Val Ala Arg Asp Gln Gly 245 250 255 Val Gly Ala Ser Phe Met Pro Lys Pro Phe Gln Glu His Ala Gly Ser 260 265 270 Ala Met His Thr His Met Ser Leu Phe Glu Gly Asp Thr Asn Ala Phe 275 280 285 His Asp Pro Asp Asp Ser Tyr Met Leu Ser Lys Thr Ala Lys Gln Phe 290 295 300 Ile Ala Gly Ile Leu His His Ala Pro Glu Phe Thr Ala Val Thr Asn 305 310 315 320 Gln Trp Val Asn Ser Tyr Lys Arg Ile Val Tyr Gly Asn Glu Ala Pro 325 330 335 Thr Ala Ala Thr Trp Gly Val Ser Asn Arg Ser Ala Leu Val Arg Val 340 345 350 Pro Thr Tyr Arg Leu Asn Lys Glu Glu Ser Arg Arg Val Glu Val Arg 355 360 365 Leu Pro Asp Thr Ala Cys Asn Pro Tyr Leu Ala Phe Ser Val Met Leu 370 375 380 Gly Ala Gly Leu Lys Gly Ile Lys Glu Gly Tyr Glu Leu Asp Glu Pro 385 390 395 400 Ala Glu Asp Asp Ile Ser Asn Leu Ser Phe Arg Glu Arg Arg Ala Met 405 410 415 Gly Tyr Asn Asp Leu Pro Ser Ser Leu Asp Gln Ala Leu Arg Gln Met 420 425 430 Glu Lys Ser Glu Leu Val Ala Asp Ile Leu Gly Glu His Val Phe Glu 435 440 445 Phe Phe Leu Arg Asn Lys Trp Arg Glu Trp Arg Asp Tyr Gln Glu Gln 450 455 460 Ile Thr Pro Trp Glu Leu Arg Asn Asn Leu Asp Tyr 465 470 475 <210> 13 <211> 1615 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1585) <223> RXA00330 <400> 13 atctttaaag cacatcctaa aaccgacaat tgaaagtgat cagcaacact ttagggtatc 60 gcgtgggcga agtcaccttt ttcaacatat ttgagacggt gtg ggg gag tat tgt 115 Val Gly Glu Tyr Cys 1 5 gtc acc cct tgg gat agg gtt ata tcc gtg gac tac att tcg acg cgt 163 Val Thr Pro Trp Asp Arg Val Ile Ser Val Asp Tyr Ile Ser Thr Arg 10 15 20 gat gcc agc cgt acc cct gcc cgc ttc agt gat att ttg ctg ggc ggt 211 Asp Ala Ser Arg Thr Pro Ala Arg Phe Ser Asp Ile Leu Leu Gly Gly 25 30 35 cta gca cca gac ggc ggc ctg tac ctg cct gca acc tac cct caa cta 259 Leu Ala Pro Asp Gly Gly Leu Tyr Leu Pro Ala Thr Tyr Pro Gln Leu 40 45 50 gat gat gcc cag ctg agt aaa tgg cgt gag gta tta gcc aac gaa gga 307 Asp Asp Ala Gln Leu Ser Lys Trp Arg Glu Val Leu Ala Asn Glu Gly 55 60 65 tac gca gct ttg gct gct gaa gtt atc tcc ctg ttt gtt gat gac atc 355 Tyr Ala Ala Leu Ala Ala Glu Val Ile Ser Leu Phe Val Asp Asp Ile 70 75 80 85 cca gta gaa gac atc aag gcg atc acc gca cgc gcc tac acc tac ccg 403 Pro Val Glu Asp Ile Lys Ala Ile Thr Ala Arg Ala Tyr Thr Tyr Pro 90 95 100 aag ttc aac agc gaa gac atc gtt cct gtc acc gaa ctc gag gac aac 451 Lys Phe Asn Ser Glu Asp Ile Val Pro Val Thr Glu Leu Glu Asp Asn 105 110 115 att tac ctg ggc cac ctt tcc gaa ggc cca acc gct gca ttc aaa gac 499 Ile Tyr Leu Gly His Leu Ser Glu Gly Pro Thr Ala Ala Phe Lys Asp 120 125 130 atg gcc atg cag ctg ctc ggc gaa ctt ttc gaa tac gag ctt cgc cgc 547 Met Ala Met Gln Leu Leu Gly Glu Leu Phe Glu Tyr Glu Leu Arg Arg 135 140 145 cgc aac gaa acc atc aac atc ctg ggc gct acc tct ggc gat acc ggc 595 Arg Asn Glu Thr Ile Asn Ile Leu Gly Ala Thr Ser Gly Asp Thr Gly 150 155 160 165 tcc tct gcg gaa tac gcc atg cgc ggc cgc gag gga atc cgc gta ttc 643 Ser Ser Ala Glu Tyr Ala Met Arg Gly Arg Glu Gly Ile Arg Val Phe 170 175 180 atg ctg acc cca gct ggc cgc atg acc cca ttc cag caa gca cag atg 691 Met Leu Thr Pro Ala Gly Arg Met Thr Pro Phe Gln Gln Ala Gln Met 185 190 195 ttt ggc ctt gac gat cca aac atc ttc aac atc gcc ctc gac ggc gtt 739 Phe Gly Leu Asp Asp Pro Asn Ile Phe Asn Ile Ala Leu Asp Gly Val 200 205 210 ttc gac gat tgc caa gac gta gtc aag gct gtc tcc gcc gac gca gaa 787 Phe Asp Asp Cys Gln Asp Val Val Lys Ala Val Ser Ala Asp Ala Glu 215 220 225 ttc aaa aaa gac aac cgc atc ggt gcc gtg aac tcc atc aac tgg gca 835 Phe Lys Lys Asp Asn Arg Ile Gly Ala Val Asn Ser Ile Asn Trp Ala 230 235 240 245 cgc ctt atg gca cag gtt gtg tac tac gtt tcc tca tgg atc cgc acc 883 Arg Leu Met Ala Gln Val Val Tyr Tyr Val Ser Ser Trp Ile Arg Thr 250 255 260 aca acc agc aat gac caa aag gtc agc ttc tcc gta cca acc ggc aac 931 Thr Thr Ser Asn Asp Gln Lys Val Ser Phe Ser Val Pro Thr Gly Asn 265 270 275 ttc ggt gac att tgc gca ggc cac atc gcc cgc caa atg gga ctt ccc 979 Phe Gly Asp Ile Cys Ala Gly His Ile Ala Arg Gln Met Gly Leu Pro 280 285 290 atc gat cgc ctc atc gtg gcc acc aac gaa aac gat gtg ctc gac gag 1027 Ile Asp Arg Leu Ile Val Ala Thr Asn Glu Asn Asp Val Leu Asp Glu 295 300 305 ttc ttc cgt acc ggc gac tac cga gtc cgc agc tcc gca gac acc cac 1075 Phe Phe Arg Thr Gly Asp Tyr Arg Val Arg Ser Ser Ala Asp Thr His 310 315 320 325 gag acc tcc tca cct tcg atg gat atc tcc cgc gcc tcc aac ttc gag 1123 Glu Thr Ser Ser Pro Ser Met Asp Ile Ser Arg Ala Ser Asn Phe Glu 330 335 340 cgt ttc atc ttc gac ctg ctc ggc cgc gac gcc acc cgc gtc aac gat 1171 Arg Phe Ile Phe Asp Leu Leu Gly Arg Asp Ala Thr Arg Val Asn Asp 345 350 355 cta ttt ggt acc cag gtt cgc caa ggc gga ttc tca ctg gct gat gac 1219 Leu Phe Gly Thr Gln Val Arg Gln Gly Gly Phe Ser Leu Ala Asp Asp 360 365 370 gcc aac ttt gag aag gct gca gca gaa tac ggt ttc gcc tcc gga cga 1267 Ala Asn Phe Glu Lys Ala Ala Ala Glu Tyr Gly Phe Ala Ser Gly Arg 375 380 385 tcc acc cat gct gac cgt gtg gca acc atc gct gac gtg cat tcc cgc 1315 Ser Thr His Ala Asp Arg Val Ala Thr Ile Ala Asp Val His Ser Arg 390 395 400 405 ctc gac gta cta atc gat ccc cac acc gcc gac ggc gtt cac gtg gca 1363 Leu Asp Val Leu Ile Asp Pro His Thr Ala Asp Gly Val His Val Ala 410 415 420 cgc cag tgg agg gac gag gtc aac acc cca atc atc gtc cta gaa act 1411 Arg Gln Trp Arg Asp Glu Val Asn Thr Pro Ile Ile Val Leu Glu Thr 425 430 435 gca ctc cca gtg aaa ttt gcc gac acc atc gtc gaa gca att ggt gaa 1459 Ala Leu Pro Val Lys Phe Ala Asp Thr Ile Val Glu Ala Ile Gly Glu 440 445 450 gca cct caa act cca gag cgt ttc gcc gcg atc atg gat gct cca ttc 1507 Ala Pro Gln Thr Pro Glu Arg Phe Ala Ala Ile Met Asp Ala Pro Phe 455 460 465 aag gtt tcc gac cta cca aac gac acc gat gca gtt aag cag tac ata 1555 Lys Val Ser Asp Leu Pro Asn Asp Thr Asp Ala Val Lys Gln Tyr Ile 470 475 480 485 gtc gat gcg att gca aac act tcc gtg aag taacttgctt tacgccaagg 1605 Val Asp Ala Ile Ala Asn Thr Ser Val Lys 490 495 cctgattcct 1615 <210> 14 <211> 495 <212> PRT <213> Corynebacterium glutamicum <400> 14 Val Gly Glu Tyr Cys Val Thr Pro Trp Asp Arg Val Ile Ser Val Asp 1 5 10 15 Tyr Ile Ser Thr Arg Asp Ala Ser Arg Thr Pro Ala Arg Phe Ser Asp 20 25 30 Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu Pro Ala 35 40 45 Thr Tyr Pro Gln Leu Asp Asp Ala Gln Leu Ser Lys Trp Arg Glu Val 50 55 60 Leu Ala Asn Glu Gly Tyr Ala Ala Leu Ala Ala Glu Val Ile Ser Leu 65 70 75 80 Phe Val Asp Asp Ile Pro Val Glu Asp Ile Lys Ala Ile Thr Ala Arg 85 90 95 Ala Tyr Thr Tyr Pro Lys Phe Asn Ser Glu Asp Ile Val Pro Val Thr 100 105 110 Glu Leu Glu Asp Asn Ile Tyr Leu Gly His Leu Ser Glu Gly Pro Thr 115 120 125 Ala Ala Phe Lys Asp Met Ala Met Gln Leu Leu Gly Glu Leu Phe Glu 130 135 140 Tyr Glu Leu Arg Arg Arg Asn Glu Thr Ile Asn Ile Leu Gly Ala Thr 145 150 155 160 Ser Gly Asp Thr Gly Ser Ser Ala Glu Tyr Ala Met Arg Gly Arg Glu 165 170 175 Gly Ile Arg Val Phe Met Leu Thr Pro Ala Gly Arg Met Thr Pro Phe 180 185 190 Gln Gln Ala Gln Met Phe Gly Leu Asp Asp Pro Asn Ile Phe Asn Ile 195 200 205 Ala Leu Asp Gly Val Phe Asp Asp Cys Gln Asp Val Val Lys Ala Val 210 215 220 Ser Ala Asp Ala Glu Phe Lys Lys Asp Asn Arg Ile Gly Ala Val Asn 225 230 235 240 Ser Ile Asn Trp Ala Arg Leu Met Ala Gln Val Val Tyr Tyr Val Ser 245 250 255 Ser Trp Ile Arg Thr Thr Thr Ser Asn Asp Gln Lys Val Ser Phe Ser 260 265 270 Val Pro Thr Gly Asn Phe Gly Asp Ile Cys Ala Gly His Ile Ala Arg 275 280 285 Gln Met Gly Leu Pro Ile Asp Arg Leu Ile Val Ala Thr Asn Glu Asn 290 295 300 Asp Val Leu Asp Glu Phe Phe Arg Thr Gly Asp Tyr Arg Val Arg Ser 305 310 315 320 Ser Ala Asp Thr His Glu Thr Ser Ser Pro Ser Met Asp Ile Ser Arg 325 330 335 Ala Ser Asn Phe Glu Arg Phe Ile Phe Asp Leu Leu Gly Arg Asp Ala 340 345 350 Thr Arg Val Asn Asp Leu Phe Gly Thr Gln Val Arg Gln Gly Gly Phe 355 360 365 Ser Leu Ala Asp Asp Ala Asn Phe Glu Lys Ala Ala Ala Glu Tyr Gly 370 375 380 Phe Ala Ser Gly Arg Ser Thr His Ala Asp Arg Val Ala Thr Ile Ala 385 390 395 400 Asp Val His Ser Arg Leu Asp Val Leu Ile Asp Pro His Thr Ala Asp 405 410 415 Gly Val His Val Ala Arg Gln Trp Arg Asp Glu Val Asn Thr Pro Ile 420 425 430 Ile Val Leu Glu Thr Ala Leu Pro Val Lys Phe Ala Asp Thr Ile Val 435 440 445 Glu Ala Ile Gly Glu Ala Pro Gln Thr Pro Glu Arg Phe Ala Ala Ile 450 455 460 Met Asp Ala Pro Phe Lys Val Ser Asp Leu Pro Asn Asp Thr Asp Ala 465 470 475 480 Val Lys Gln Tyr Ile Val Asp Ala Ile Ala Asn Thr Ser Val Lys 485 490 495 <210> 15 <211> 1474 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1444) <223> RXA00383 <400> 15 ccatatcttt aaccttggtc atggtgtgct tcctaatacg gtggcggaag atattactga 60 agccgtctcc atcattcatt cttaaactaa gaggagtttc atg cgt ttt gcc atc 115 Met Arg Phe Ala Ile 1 5 atc ggt gca ggc ctt gcg ggt ctg act gct gca tat gag atc cat aaa 163 Ile Gly Ala Gly Leu Ala Gly Leu Thr Ala Ala Tyr Glu Ile His Lys 10 15 20 gct gat ccc act gcc caa atc gat gtg ttg gaa gca ggc gaa cgc att 211 Ala Asp Pro Thr Ala Gln Ile Asp Val Leu Glu Ala Gly Glu Arg Ile 25 30 35 ggc ggc aag ctt ttt acg gtg ccg ttt gct tcc gga cct acc gat att 259 Gly Gly Lys Leu Phe Thr Val Pro Phe Ala Ser Gly Pro Thr Asp Ile 40 45 50 gga gcg gag gcg ttt tta gct gcg cgt tcc gat gcg gtg gag ttt ttt 307 Gly Ala Glu Ala Phe Leu Ala Ala Arg Ser Asp Ala Val Glu Phe Phe 55 60 65 act gag ctt ggg ttg gct gat tct ttg gtc agc ccg tct gct gcg aag 355 Thr Glu Leu Gly Leu Ala Asp Ser Leu Val Ser Pro Ser Ala Ala Lys 70 75 80 85 tct cag tat ttc gcg ggc ggt gcg ctg cat gcg ttc ccc gca ggt gga 403 Ser Gln Tyr Phe Ala Gly Gly Ala Leu His Ala Phe Pro Ala Gly Gly 90 95 100 gtg atg ggt att cca agc aat cca cca gca ggc gcg caa gac acc gct 451 Val Met Gly Ile Pro Ser Asn Pro Pro Ala Gly Ala Gln Asp Thr Ala 105 110 115 ttt gat tgg act cct ggc caa gac att tct gtt ggc gcc tta gtg cgc 499 Phe Asp Trp Thr Pro Gly Gln Asp Ile Ser Val Gly Ala Leu Val Arg 120 125 130 cgt cag tat ggc gat gag atc gtc gat act gtg gtg tct tcg ctg ctt 547 Arg Gln Tyr Gly Asp Glu Ile Val Asp Thr Val Val Ser Ser Leu Leu 135 140 145 ggt ggc gtt tat tcc tcc acc gct gat gat ctg ggt gtg cgc gct tcc 595 Gly Gly Val Tyr Ser Ser Thr Ala Asp Asp Leu Gly Val Arg Ala Ser 150 155 160 165 gtg ccg gca ctt gct gca gcc ctt gat cag ctg gct gag gcc ggc gag 643 Val Pro Ala Leu Ala Ala Ala Leu Asp Gln Leu Ala Glu Ala Gly Glu 170 175 180 ccg gtg act ctg tca gct gcg gtc aag gcc gtg gaa gct cag cgg gaa 691 Pro Val Thr Leu Ser Ala Ala Val Lys Ala Val Glu Ala Gln Arg Glu 185 190 195 gcc gca aaa aca act tca gaa acc cgc ccc gtt ttc cag acc ttc aag 739 Ala Ala Lys Thr Thr Ser Glu Thr Arg Pro Val Phe Gln Thr Phe Lys 200 205 210 ggc gga tac gcg gag ctg tac gaa gcg ttg gca gag caa tgc ggt gca 787 Gly Gly Tyr Ala Glu Leu Tyr Glu Ala Leu Ala Glu Gln Cys Gly Ala 215 220 225 gat att cac ttg gat agt ttc gtt tcc gcc atc acc aaa gat ggt gaa 835 Asp Ile His Leu Asp Ser Phe Val Ser Ala Ile Thr Lys Asp Gly Glu 230 235 240 245 ggt ttt gcc atc aag ggc ggt ggc gaa ggc acc tac gac aag gtg att 883 Gly Phe Ala Ile Lys Gly Gly Gly Glu Gly Thr Tyr Asp Lys Val Ile 250 255 260 ttg gcg gtt ccc gct cca acc gcc gct gtg ctg ctc cgc gac ttg gca 931 Leu Ala Val Pro Ala Pro Thr Ala Ala Val Leu Leu Arg Asp Leu Ala 265 270 275 ccg gcc gca gcg cca cat ttg cgc gca att aag ttg gct tct tca gca 979 Pro Ala Ala Ala Pro His Leu Arg Ala Ile Lys Leu Ala Ser Ser Ala 280 285 290 gtc gtc ggc atg cgt ttc gat tcc agc gag ggc ctg ccc gac aac tcc 1027 Val Val Gly Met Arg Phe Asp Ser Ser Glu Gly Leu Pro Asp Asn Ser 295 300 305 ggc gtc ctg gtc gct gtt aat gag ccg ggc atc acg gcg aag gcc ttc 1075 Gly Val Leu Val Ala Val Asn Glu Pro Gly Ile Thr Ala Lys Ala Phe 310 315 320 325 acg ttc tcc tca aag aag tgg cct cac ctg gag gct cgc ggg ggc gcg 1123 Thr Phe Ser Ser Lys Lys Trp Pro His Leu Glu Ala Arg Gly Gly Ala 330 335 340 ctc gtg cgc gcg tcg ttc ggc agg cta ggc gat gag gcg tcg gca cgc 1171 Leu Val Arg Ala Ser Phe Gly Arg Leu Gly Asp Glu Ala Ser Ala Arg 345 350 355 atg gac gag gat ttg ctt gtc gac gcc gcc ctc gac gat ctc ctc acc 1219 Met Asp Glu Asp Leu Leu Val Asp Ala Ala Leu Asp Asp Leu Leu Thr 360 365 370 ata acc ggg ttc gac ggc cgg gct gcc gga ctg ggt gaa att ttc gtg 1267 Ile Thr Gly Phe Asp Gly Arg Ala Ala Gly Leu Gly Glu Ile Phe Val 375 380 385 cag cgc tgg ttc ggt ggg ctc cca gcc tat gga gtt gat cac att gct 1315 Gln Arg Trp Phe Gly Gly Leu Pro Ala Tyr Gly Val Asp His Ile Ala 390 395 400 405 acc gtt tcg gct gcg cgt gca gag atc gca gcc gtg cct ggc gtg gaa 1363 Thr Val Ser Ala Ala Arg Ala Glu Ile Ala Ala Val Pro Gly Val Glu 410 415 420 gca att ggc gcg tgg gct ggg gga gtg gga gtt ccc gca gtt atc gca 1411 Ala Ile Gly Ala Trp Ala Gly Gly Val Gly Val Pro Ala Val Ile Ala 425 430 435 gat gcc cag gca gca gta cac agg ttg ctg gga taagcaccca aaaacactat 1464 Asp Ala Gln Ala Ala Val His Arg Leu Leu Gly 440 445 tgaccacccc 1474 <210> 16 <211> 448 <212> PRT <213> Corynebacterium glutamicum <400> 16 Met Arg Phe Ala Ile Ile Gly Ala Gly Leu Ala Gly Leu Thr Ala Ala 1 5 10 15 Tyr Glu Ile His Lys Ala Asp Pro Thr Ala Gln Ile Asp Val Leu Glu 20 25 30 Ala Gly Glu Arg Ile Gly Gly Lys Leu Phe Thr Val Pro Phe Ala Ser 35 40 45 Gly Pro Thr Asp Ile Gly Ala Glu Ala Phe Leu Ala Ala Arg Ser Asp 50 55 60 Ala Val Glu Phe Phe Thr Glu Leu Gly Leu Ala Asp Ser Leu Val Ser 65 70 75 80 Pro Ser Ala Ala Lys Ser Gln Tyr Phe Ala Gly Gly Ala Leu His Ala 85 90 95 Phe Pro Ala Gly Gly Val Met Gly Ile Pro Ser Asn Pro Pro Ala Gly 100 105 110 Ala Gln Asp Thr Ala Phe Asp Trp Thr Pro Gly Gln Asp Ile Ser Val 115 120 125 Gly Ala Leu Val Arg Arg Gln Tyr Gly Asp Glu Ile Val Asp Thr Val 130 135 140 Val Ser Ser Leu Leu Gly Gly Val Tyr Ser Ser Thr Ala Asp Asp Leu 145 150 155 160 Gly Val Arg Ala Ser Val Pro Ala Leu Ala Ala Ala Leu Asp Gln Leu 165 170 175 Ala Glu Ala Gly Glu Pro Val Thr Leu Ser Ala Ala Val Lys Ala Val 180 185 190 Glu Ala Gln Arg Glu Ala Ala Lys Thr Thr Ser Glu Thr Arg Pro Val 195 200 205 Phe Gln Thr Phe Lys Gly Gly Tyr Ala Glu Leu Tyr Glu Ala Leu Ala 210 215 220 Glu Gln Cys Gly Ala Asp Ile His Leu Asp Ser Phe Val Ser Ala Ile 225 230 235 240 Thr Lys Asp Gly Glu Gly Phe Ala Ile Lys Gly Gly Gly Glu Gly Thr 245 250 255 Tyr Asp Lys Val Ile Leu Ala Val Pro Ala Pro Thr Ala Ala Val Leu 260 265 270 Leu Arg Asp Leu Ala Pro Ala Ala Ala Pro His Leu Arg Ala Ile Lys 275 280 285 Leu Ala Ser Ser Ala Val Val Gly Met Arg Phe Asp Ser Ser Glu Gly 290 295 300 Leu Pro Asp Asn Ser Gly Val Leu Val Ala Val Asn Glu Pro Gly Ile 305 310 315 320 Thr Ala Lys Ala Phe Thr Phe Ser Ser Lys Lys Trp Pro His Leu Glu 325 330 335 Ala Arg Gly Gly Ala Leu Val Arg Ala Ser Phe Gly Arg Leu Gly Asp 340 345 350 Glu Ala Ser Ala Arg Met Asp Glu Asp Leu Leu Val Asp Ala Ala Leu 355 360 365 Asp Asp Leu Leu Thr Ile Thr Gly Phe Asp Gly Arg Ala Ala Gly Leu 370 375 380 Gly Glu Ile Phe Val Gln Arg Trp Phe Gly Gly Leu Pro Ala Tyr Gly 385 390 395 400 Val Asp His Ile Ala Thr Val Ser Ala Ala Arg Ala Glu Ile Ala Ala 405 410 415 Val Pro Gly Val Glu Ala Ile Gly Ala Trp Ala Gly Gly Val Gly Val 420 425 430 Pro Ala Val Ile Ala Asp Ala Gln Ala Ala Val His Arg Leu Leu Gly 435 440 445 <210> 17 <211> 1690 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1660) <223> RXA00389 <400> 17 ccaccactgc gtaacctttc cgagcaagat atcgcggacc tgtcggattt gcttgccacc 60 tctggcgcag gttcctaccg ccttcagttg aggtgaaagc atg atc acc gca acc 115 Met Ile Thr Ala Thr 1 5 gca ctg cat ggg tgt tca ctg att gat ggc gag tgg gtc gct gga aaa 163 Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu Trp Val Ala Gly Lys 10 15 20 aat ggt gag att aca gga ttc gat ccg cgc acc aat gcg agt ctg aac 211 Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr Asn Ala Ser Leu Asn 25 30 35 cct tcc tac tct tta gca aac agc gca cag ctg cgc gcc gcc aca aca 259 Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu Arg Ala Ala Thr Thr 40 45 50 tcg gcg aag cga gct ttt gaa agc tac cga ctc act act cca gag gtt 307 Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu Thr Thr Pro Glu Val 55 60 65 aga gca gat ttc ctg gat tcc atc gct gac aac atc gat gcg cta tcc 355 Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn Ile Asp Ala Leu Ser 70 75 80 85 ggc gag atc gtg caa cgg gcg agc ctg gag aca ggt ttg gga act acc 403 Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr Gly Leu Gly Thr Thr 90 95 100 cga ctc aca ggc gaa gta gcc cgc acc agc aac cag ctc cgc ctg ttt 451 Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn Gln Leu Arg Leu Phe 105 110 115 gca gaa acc gtg aga agc gga cag ttc cac cga gta cgc att gaa cga 499 Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg Val Arg Ile Glu Arg 120 125 130 gga ccg cgg att gat ctt cgc cag cgt cag gtt ccg ttg gga cca gtc 547 Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val Pro Leu Gly Pro Val 135 140 145 gcg gta ttc ggg gca agc aac ttc ccc gtc gct ttc tct act gct ggt 595 Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala Phe Ser Thr Ala Gly 150 155 160 165 ggc gat aca gca tca gcg ttg gct gca ggc tgc cct gtg gtt ttt aag 643 Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys Pro Val Val Phe Lys 170 175 180 gcg cat aat gcg cac cct gga aca gct gag ctc gtc ggg caa gcg gtg 691 Ala His Asn Ala His Pro Gly Thr Ala Glu Leu Val Gly Gln Ala Val 185 190 195 cgg gga gcc gtc gaa aag cat gag ttt gat gct ggt gtg ttt aac ctt 739 Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala Gly Val Phe Asn Leu 200 205 210 gtc tac ggc cgt ggc gtg gaa att ggc cag gag ctg gct gcg gat ccg 787 Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu Leu Ala Ala Asp Pro 215 220 225 aat atc acg gca atc ggt ttt acc ggt tca cgc cag ggt ggt ttg gca 835 Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg Gln Gly Gly Leu Ala 230 235 240 245 ctg tca cag act gcg ttt agc cgc cca gtt ccc gtt cca gtc ttt gca 883 Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro Val Pro Val Phe Ala 250 255 260 gaa atg agt gcc acc aac cct gtg ttc gtc ttc ccc ggc gcg ctg gcg 931 Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe Pro Gly Ala Leu Ala 265 270 275 gat ttg gat gca tcg agt tcc ttg gct gag gcg ttt acc gct tcc gtc 979 Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala Phe Thr Ala Ser Val 280 285 290 acc ggc agt tcc ggg caa ttg tgc acc aag cct ggc ctc gtt ttc atc 1027 Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro Gly Leu Val Phe Ile 295 300 305 ccg cgc ggt gtt gtt ggt gat gct ttt gtg gcg ctc gta gca gcc aaa 1075 Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala Leu Val Ala Ala Lys 310 315 320 325 ttt aaa gaa acc acg ggt caa acg atg ctc acg caa ggc atc gct cag 1123 Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr Gln Gly Ile Ala Gln 330 335 340 gca tgg cag cgc gga gtc gac aac ctt gca gca cag cca agt gta aaa 1171 Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala Gln Pro Ser Val Lys 345 350 355 atc ctc gcc caa ggc acc ccc gga gat gga gag aac gcg ccg ggc ccg 1219 Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu Asn Ala Pro Gly Pro 360 365 370 gtg gtg ttt gaa agt gat gtg cag gcg ttg cta aat aat gtg gtg ttg 1267 Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu Asn Asn Val Val Leu 375 380 385 cag gaa gaa atc ttc ggt gcg gca tcg ctg gtg gtg cgt tat gat tcc 1315 Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val Val Arg Tyr Asp Ser 390 395 400 405 ccg gat caa ctc cac caa gta gcc aat tca ctc gag gga caa tta aca 1363 Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu Glu Gly Gln Leu Thr 410 415 420 gcc acg atc cac gca tcc cag gat gat ttc cag gaa gtc tcg aaa ctt 1411 Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln Glu Val Ser Lys Leu 425 430 435 atc ccc ctc ttg gag gat ctc gcg ggc cgt gtt ctt tac ggc ggc tgg 1459 Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val Leu Tyr Gly Gly Trp 440 445 450 cca acg ggt gtg gaa gtt ggg cac acg gtt atc cat gga ggc cct tat 1507 Pro Thr Gly Val Glu Val Gly His Thr Val Ile His Gly Gly Pro Tyr 455 460 465 ccg gcg acc tca aat gcg cag tcg aca agt gtt gga acc ctg gca atc 1555 Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val Gly Thr Leu Ala Ile 470 475 480 485 gag aga ttt atg cgc ccg gtt tct tat caa act ttc ccg gct gag ctg 1603 Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr Phe Pro Ala Glu Leu 490 495 500 ctt cca gat cca gtt tct gag gcg aat aaa tgg gct gta cct cgg gaa 1651 Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp Ala Val Pro Arg Glu 505 510 515 ata gac cgt taatagctgg tctttacatt tgccagaaag 1690 Ile Asp Arg 520 <210> 18 <211> 520 <212> PRT <213> Corynebacterium glutamicum <400> 18 Met Ile Thr Ala Thr Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu 1 5 10 15 Trp Val Ala Gly Lys Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr 20 25 30 Asn Ala Ser Leu Asn Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu 35 40 45 Arg Ala Ala Thr Thr Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu 50 55 60 Thr Thr Pro Glu Val Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn 65 70 75 80 Ile Asp Ala Leu Ser Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr 85 90 95 Gly Leu Gly Thr Thr Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn 100 105 110 Gln Leu Arg Leu Phe Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg 115 120 125 Val Arg Ile Glu Arg Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val 130 135 140 Pro Leu Gly Pro Val Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala 145 150 155 160 Phe Ser Thr Ala Gly Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys 165 170 175 Pro Val Val Phe Lys Ala His Asn Ala His Pro Gly Thr Ala Glu Leu 180 185 190 Val Gly Gln Ala Val Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala 195 200 205 Gly Val Phe Asn Leu Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu 210 215 220 Leu Ala Ala Asp Pro Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg 225 230 235 240 Gln Gly Gly Leu Ala Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro 245 250 255 Val Pro Val Phe Ala Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe 260 265 270 Pro Gly Ala Leu Ala Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala 275 280 285 Phe Thr Ala Ser Val Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro 290 295 300 Gly Leu Val Phe Ile Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala 305 310 315 320 Leu Val Ala Ala Lys Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr 325 330 335 Gln Gly Ile Ala Gln Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala 340 345 350 Gln Pro Ser Val Lys Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu 355 360 365 Asn Ala Pro Gly Pro Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu 370 375 380 Asn Asn Val Val Leu Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val 385 390 395 400 Val Arg Tyr Asp Ser Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu 405 410 415 Glu Gly Gln Leu Thr Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln 420 425 430 Glu Val Ser Lys Leu Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val 435 440 445 Leu Tyr Gly Gly Trp Pro Thr Gly Val Glu Val Gly His Thr Val Ile 450 455 460 His Gly Gly Pro Tyr Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val 465 470 475 480 Gly Thr Leu Ala Ile Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr 485 490 495 Phe Pro Ala Glu Leu Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp 500 505 510 Ala Val Pro Arg Glu Ile Asp Arg 515 520 <210> 19 <211> 2161 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(2131) <223> RXA00416 <400> 19 caagctctga aagcagatag cacgtgagac tcattaatac atacttgtaa ttttgctcct 60 cgatcccagc aatgctcgtt gagtatgaaa gacttctatc atg att aac acc tct 115 Met Ile Asn Thr Ser 1 5 gaa gat gaa ggt gca gtc gtg gtt cat gag gat ttg aat cct tat ggt 163 Glu Asp Glu Gly Ala Val Val Val His Glu Asp Leu Asn Pro Tyr Gly 10 15 20 cca tca cgt gtg cct gaa ggc gaa gtg tat cgg cct gat cgt cta aac 211 Pro Ser Arg Val Pro Glu Gly Glu Val Tyr Arg Pro Asp Arg Leu Asn 25 30 35 agg aaa gct aca ctt gtt gca att gtg gga gct gcc atc ctg gct ttc 259 Arg Lys Ala Thr Leu Val Ala Ile Val Gly Ala Ala Ile Leu Ala Phe 40 45 50 gca ttt gct ttg gtg ttg tgg atg ggt tta aaa caa aca aac ctt cca 307 Ala Phe Ala Leu Val Leu Trp Met Gly Leu Lys Gln Thr Asn Leu Pro 55 60 65 gct ttc ggc cca tcg aat gtc acg agg gct gtt gct tct gcc acc atc 355 Ala Phe Gly Pro Ser Asn Val Thr Arg Ala Val Ala Ser Ala Thr Ile 70 75 80 85 gct gcg gtt ttg att gtc acg ggt ttt ttg acg tgg ctg tgg ctg cgc 403 Ala Ala Val Leu Ile Val Thr Gly Phe Leu Thr Trp Leu Trp Leu Arg 90 95 100 gat gaa cat cag agc aat ccg cgg tgg gag ttg gag gac gtc aag cca 451 Asp Glu His Gln Ser Asn Pro Arg Trp Glu Leu Glu Asp Val Lys Pro 105 110 115 agg ccc aag tgg cgg acg gcg ctg acg tat ctg gcg tct tac tta agc 499 Arg Pro Lys Trp Arg Thr Ala Leu Thr Tyr Leu Ala Ser Tyr Leu Ser 120 125 130 cca gct gcg ctg gtg gtt gcg gtg ttg gct att ccg ctg tct gcg acc 547 Pro Ala Ala Leu Val Val Ala Val Leu Ala Ile Pro Leu Ser Ala Thr 135 140 145 cgc ctg tat ttg gac gga atc agc gtt gac cag ggc ttt aga act cag 595 Arg Leu Tyr Leu Asp Gly Ile Ser Val Asp Gln Gly Phe Arg Thr Gln 150 155 160 165 ttt tta acc cgc atg gct gac gat atc ggc ttg tcg gac atg aac tac 643 Phe Leu Thr Arg Met Ala Asp Asp Ile Gly Leu Ser Asp Met Asn Tyr 170 175 180 atc gat atg cct acc ttc tac cct gct gga tgg ttc tgg ctc ggt ggt 691 Ile Asp Met Pro Thr Phe Tyr Pro Ala Gly Trp Phe Trp Leu Gly Gly 185 190 195 cgc ttg gcc aat ctt ttg ggg ctg ccc ggt tgg gaa gct ttc cag cca 739 Arg Leu Ala Asn Leu Leu Gly Leu Pro Gly Trp Glu Ala Phe Gln Pro 200 205 210 tgg gca att gtg tcc atg gca gtt gct gct tct gtg tta gtt cca gtg 787 Trp Ala Ile Val Ser Met Ala Val Ala Ala Ser Val Leu Val Pro Val 215 220 225 tgg cag cgc atc acc ggt tcc ctg ccg gtg gca aca ggc att gcg ttg 835 Trp Gln Arg Ile Thr Gly Ser Leu Pro Val Ala Thr Gly Ile Ala Leu 230 235 240 245 gtg aca acc tgc att atc ttg gcg atg aat tcc gaa gag ccc tac gct 883 Val Thr Thr Cys Ile Ile Leu Ala Met Asn Ser Glu Glu Pro Tyr Ala 250 255 260 gca atc gtt gcg atg ggt att cca gcg atg ctc gtg ctg gct tcc cgc 931 Ala Ile Val Ala Met Gly Ile Pro Ala Met Leu Val Leu Ala Ser Arg 265 270 275 att gcc aag ggc gat aag ttt gcg ctt gcc ggc ggc att att tac ttg 979 Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala Gly Gly Ile Ile Tyr Leu 280 285 290 ggt gtt tcg gct act ttc tat act ttg ttc acc ggt gct atc gcg ctt 1027 Gly Val Ser Ala Thr Phe Tyr Thr Leu Phe Thr Gly Ala Ile Ala Leu 295 300 305 tct gcg gtc gcg gtg tgc atc gtg gtg gcg gct att gtg cag cgc tcc 1075 Ser Ala Val Ala Val Cys Ile Val Val Ala Ala Ile Val Gln Arg Ser 310 315 320 325 atc aaa cca ctg ctg tgg ctt gca gtg ctg ggt ggt gga tcc att gtc 1123 Ile Lys Pro Leu Leu Trp Leu Ala Val Leu Gly Gly Gly Ser Ile Val 330 335 340 att gcg ttg att tct tgg ggt cct tac ctt ctg gcc tcc atc aac gga 1171 Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu Leu Ala Ser Ile Asn Gly 345 350 355 gcg gag cgc tct ggc gat tcc gca aca cac tac ctg cct ctt gaa ggc 1219 Ala Glu Arg Ser Gly Asp Ser Ala Thr His Tyr Leu Pro Leu Glu Gly 360 365 370 acc caa ttc ccg gtt cct ttc ttg gca tca agc gtt gtg gga ctg ttg 1267 Thr Gln Phe Pro Val Pro Phe Leu Ala Ser Ser Val Val Gly Leu Leu 375 380 385 tgt ctt gtt ggc ctg atc tat ttg gtg gtg cgt ttc cac aac aat gag 1315 Cys Leu Val Gly Leu Ile Tyr Leu Val Val Arg Phe His Asn Asn Glu 390 395 400 405 gtg cgc gcg atg tgg gtc ggc atc gca gtg ttt tat gcc tgg atg ggc 1363 Val Arg Ala Met Trp Val Gly Ile Ala Val Phe Tyr Ala Trp Met Gly 410 415 420 atg tcc atg gcg atc acg ctt ttg ggc aac acg ttg ctt gga ttc cgt 1411 Met Ser Met Ala Ile Thr Leu Leu Gly Asn Thr Leu Leu Gly Phe Arg 425 430 435 ctt gat acg gtg ctg gtg ctt att ttt gcc acg gct gga gtg ttg ggc 1459 Leu Asp Thr Val Leu Val Leu Ile Phe Ala Thr Ala Gly Val Leu Gly 440 445 450 att gca gat ttc cgc ctt gcc agt gtg tat cag ctc tac ccc acc caa 1507 Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr Gln Leu Tyr Pro Thr Gln 455 460 465 atc aca gag cgc acg gcc acc cat ctg acc aat cta att gtg gtc ctc 1555 Ile Thr Glu Arg Thr Ala Thr His Leu Thr Asn Leu Ile Val Val Leu 470 475 480 485 gtg ctg ctt ggc ggc ctc tac tac gcg caa gat ctg ccg cag aag aac 1603 Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gln Asp Leu Pro Gln Lys Asn 490 495 500 gca cga gct atc gat ctg gcc tat acc gat act gat ggc tac ggc gag 1651 Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp Thr Asp Gly Tyr Gly Glu 505 510 515 cgc gcg gat ctg tat ccg gcc gga gct gca cgt tat tac aag gac atc 1699 Arg Ala Asp Leu Tyr Pro Ala Gly Ala Ala Arg Tyr Tyr Lys Asp Ile 520 525 530 aac gat cat ctg ctt gat caa gga ttc gag cct tcc gaa act gtc gtg 1747 Asn Asp His Leu Leu Asp Gln Gly Phe Glu Pro Ser Glu Thr Val Val 535 540 545 ctg aca gac gaa ctc gat ttc atg tcc tac tac cct tat cgc gga tac 1795 Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr Tyr Pro Tyr Arg Gly Tyr 550 555 560 565 caa gct ttt act tcc cac tac gcc aac ccg ctt ggt gag ttc gga aac 1843 Gln Ala Phe Thr Ser His Tyr Ala Asn Pro Leu Gly Glu Phe Gly Asn 570 575 580 agg aac gca ttc atc gaa gat ctc gcg atc cga agc tgg gat gag ttg 1891 Arg Asn Ala Phe Ile Glu Asp Leu Ala Ile Arg Ser Trp Asp Glu Leu 585 590 595 gct gat cct caa caa ttc agc gac gcc ttg aac acc tct cca tgg acg 1939 Ala Asp Pro Gln Gln Phe Ser Asp Ala Leu Asn Thr Ser Pro Trp Thr 600 605 610 atc cct gag gtg ttc atc ttc cgt ggc tcc atc gat gat cct gac gcc 1987 Ile Pro Glu Val Phe Ile Phe Arg Gly Ser Ile Asp Asp Pro Asp Ala 615 620 625 ggt tgg aaa tac gac gtg gct gaa gat ctg tac ccg aac aat cca aac 2035 Gly Trp Lys Tyr Asp Val Ala Glu Asp Leu Tyr Pro Asn Asn Pro Asn 630 635 640 645 gtg cgc ttc cgc ggc gtg tac ttt aac ccg gag tca ttt gat cag atg 2083 Val Arg Phe Arg Gly Val Tyr Phe Asn Pro Glu Ser Phe Asp Gln Met 650 655 660 tgg cag acc aag caa gtg gga cct ttc gtg gtg gta acg cac aat gag 2131 Trp Gln Thr Lys Gln Val Gly Pro Phe Val Val Val Thr His Asn Glu 665 670 675 taattcctca ccaaacgacc caagccctat 2161 <210> 20 <211> 677 <212> PRT <213> Corynebacterium glutamicum <400> 20 Met Ile Asn Thr Ser Glu Asp Glu Gly Ala Val Val Val His Glu Asp 1 5 10 15 Leu Asn Pro Tyr Gly Pro Ser Arg Val Pro Glu Gly Glu Val Tyr Arg 20 25 30 Pro Asp Arg Leu Asn Arg Lys Ala Thr Leu Val Ala Ile Val Gly Ala 35 40 45 Ala Ile Leu Ala Phe Ala Phe Ala Leu Val Leu Trp Met Gly Leu Lys 50 55 60 Gln Thr Asn Leu Pro Ala Phe Gly Pro Ser Asn Val Thr Arg Ala Val 65 70 75 80 Ala Ser Ala Thr Ile Ala Ala Val Leu Ile Val Thr Gly Phe Leu Thr 85 90 95 Trp Leu Trp Leu Arg Asp Glu His Gln Ser Asn Pro Arg Trp Glu Leu 100 105 110 Glu Asp Val Lys Pro Arg Pro Lys Trp Arg Thr Ala Leu Thr Tyr Leu 115 120 125 Ala Ser Tyr Leu Ser Pro Ala Ala Leu Val Val Ala Val Leu Ala Ile 130 135 140 Pro Leu Ser Ala Thr Arg Leu Tyr Leu Asp Gly Ile Ser Val Asp Gln 145 150 155 160 Gly Phe Arg Thr Gln Phe Leu Thr Arg Met Ala Asp Asp Ile Gly Leu 165 170 175 Ser Asp Met Asn Tyr Ile Asp Met Pro Thr Phe Tyr Pro Ala Gly Trp 180 185 190 Phe Trp Leu Gly Gly Arg Leu Ala Asn Leu Leu Gly Leu Pro Gly Trp 195 200 205 Glu Ala Phe Gln Pro Trp Ala Ile Val Ser Met Ala Val Ala Ala Ser 210 215 220 Val Leu Val Pro Val Trp Gln Arg Ile Thr Gly Ser Leu Pro Val Ala 225 230 235 240 Thr Gly Ile Ala Leu Val Thr Thr Cys Ile Ile Leu Ala Met Asn Ser 245 250 255 Glu Glu Pro Tyr Ala Ala Ile Val Ala Met Gly Ile Pro Ala Met Leu 260 265 270 Val Leu Ala Ser Arg Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala Gly 275 280 285 Gly Ile Ile Tyr Leu Gly Val Ser Ala Thr Phe Tyr Thr Leu Phe Thr 290 295 300 Gly Ala Ile Ala Leu Ser Ala Val Ala Val Cys Ile Val Val Ala Ala 305 310 315 320 Ile Val Gln Arg Ser Ile Lys Pro Leu Leu Trp Leu Ala Val Leu Gly 325 330 335 Gly Gly Ser Ile Val Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu Leu 340 345 350 Ala Ser Ile Asn Gly Ala Glu Arg Ser Gly Asp Ser Ala Thr His Tyr 355 360 365 Leu Pro Leu Glu Gly Thr Gln Phe Pro Val Pro Phe Leu Ala Ser Ser 370 375 380 Val Val Gly Leu Leu Cys Leu Val Gly Leu Ile Tyr Leu Val Val Arg 385 390 395 400 Phe His Asn Asn Glu Val Arg Ala Met Trp Val Gly Ile Ala Val Phe 405 410 415 Tyr Ala Trp Met Gly Met Ser Met Ala Ile Thr Leu Leu Gly Asn Thr 420 425 430 Leu Leu Gly Phe Arg Leu Asp Thr Val Leu Val Leu Ile Phe Ala Thr 435 440 445 Ala Gly Val Leu Gly Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr Gln 450 455 460 Leu Tyr Pro Thr Gln Ile Thr Glu Arg Thr Ala Thr His Leu Thr Asn 465 470 475 480 Leu Ile Val Val Leu Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gln Asp 485 490 495 Leu Pro Gln Lys Asn Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp Thr 500 505 510 Asp Gly Tyr Gly Glu Arg Ala Asp Leu Tyr Pro Ala Gly Ala Ala Arg 515 520 525 Tyr Tyr Lys Asp Ile Asn Asp His Leu Leu Asp Gln Gly Phe Glu Pro 530 535 540 Ser Glu Thr Val Val Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr Tyr 545 550 555 560 Pro Tyr Arg Gly Tyr Gln Ala Phe Thr Ser His Tyr Ala Asn Pro Leu 565 570 575 Gly Glu Phe Gly Asn Arg Asn Ala Phe Ile Glu Asp Leu Ala Ile Arg 580 585 590 Ser Trp Asp Glu Leu Ala Asp Pro Gln Gln Phe Ser Asp Ala Leu Asn 595 600 605 Thr Ser Pro Trp Thr Ile Pro Glu Val Phe Ile Phe Arg Gly Ser Ile 610 615 620 Asp Asp Pro Asp Ala Gly Trp Lys Tyr Asp Val Ala Glu Asp Leu Tyr 625 630 635 640 Pro Asn Asn Pro Asn Val Arg Phe Arg Gly Val Tyr Phe Asn Pro Glu 645 650 655 Ser Phe Asp Gln Met Trp Gln Thr Lys Gln Val Gly Pro Phe Val Val 660 665 670 Val Thr His Asn Glu 675 <210> 21 <211> 1252 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1222) <223> RXA00489 <400> 21 cagcccgatt tcttcattga aatcgggctg tttctggttg tgtcgcgtat ctcgggtaaa 60 gtcttcgtcg tatacgacca tttaagggag gcccgtcaca atg cgt gac cac gtt 115 Met Arg Asp His Val 1 5 gaa atc ggt atc ggc cgt gag gca cga cgc acc tac agc ttg gac gat 163 Glu Ile Gly Ile Gly Arg Glu Ala Arg Arg Thr Tyr Ser Leu Asp Asp 10 15 20 att tct gtc gtt tct agc cgc cgc acc cgt tca tcc aaa gat gtc gac 211 Ile Ser Val Val Ser Ser Arg Arg Thr Arg Ser Ser Lys Asp Val Asp 25 30 35 acc act tgg cat att gac gcc tac aag ttt gat ctg ccg ttc atg aat 259 Thr Thr Trp His Ile Asp Ala Tyr Lys Phe Asp Leu Pro Phe Met Asn 40 45 50 cac cca agt gat gca ttg gca agc cct gag ttt gtc att gaa atg ggc 307 His Pro Ser Asp Ala Leu Ala Ser Pro Glu Phe Val Ile Glu Met Gly 55 60 65 aag cag ggt ggc ctt ggc gtg atc aac gct gag ggt ctg tgg ggt cgc 355 Lys Gln Gly Gly Leu Gly Val Ile Asn Ala Glu Gly Leu Trp Gly Arg 70 75 80 85 cat gct gat ctc gat gag gcg atc gca aag gtg att gct gcg tat gag 403 His Ala Asp Leu Asp Glu Ala Ile Ala Lys Val Ile Ala Ala Tyr Glu 90 95 100 gaa ggc gac cag gct gca gcc act cgc act ctt cag gag ctg cac gca 451 Glu Gly Asp Gln Ala Ala Ala Thr Arg Thr Leu Gln Glu Leu His Ala 105 110 115 gcg cca ctg gat act gag ctg ctg agt gag cgc att gcg cag gtt cgt 499 Ala Pro Leu Asp Thr Glu Leu Leu Ser Glu Arg Ile Ala Gln Val Arg 120 125 130 gat tcc ggt gag atc gtt gct gtg cgc gtg tct cca caa aat gtt cgt 547 Asp Ser Gly Glu Ile Val Ala Val Arg Val Ser Pro Gln Asn Val Arg 135 140 145 gag atc gca cca atc gtc atc aag gca ggt gct gat ctg ctg gtt atc 595 Glu Ile Ala Pro Ile Val Ile Lys Ala Gly Ala Asp Leu Leu Val Ile 150 155 160 165 cag ggc acc ctg atc tct gca gag cac gtc aac acc ggt gga gag gcc 643 Gln Gly Thr Leu Ile Ser Ala Glu His Val Asn Thr Gly Gly Glu Ala 170 175 180 ctg aac cta aag gaa ttc atc ggt tct ttg gat gtt cct gtc atc gct 691 Leu Asn Leu Lys Glu Phe Ile Gly Ser Leu Asp Val Pro Val Ile Ala 185 190 195 ggt ggc gtg aac gat tac acc acc gcg ttg cac atg atg cgt acc ggt 739 Gly Gly Val Asn Asp Tyr Thr Thr Ala Leu His Met Met Arg Thr Gly 200 205 210 gct gtg ggc atc atc gtc ggt ggc ggc gag aac acc aac agc cta gca 787 Ala Val Gly Ile Ile Val Gly Gly Gly Glu Asn Thr Asn Ser Leu Ala 215 220 225 ttg ggc atg gag gta tcc atg gcc act gcg att gct gat gtc gct gcg 835 Leu Gly Met Glu Val Ser Met Ala Thr Ala Ile Ala Asp Val Ala Ala 230 235 240 245 gca cgt cgt gat tac ctg gat gag acc ggt gga cgt tac gtg cac atc 883 Ala Arg Arg Asp Tyr Leu Asp Glu Thr Gly Gly Arg Tyr Val His Ile 250 255 260 att gca gat gga agc att gaa aac tcc ggt gat gta gtc aag gct att 931 Ile Ala Asp Gly Ser Ile Glu Asn Ser Gly Asp Val Val Lys Ala Ile 265 270 275 gcc tgt ggc gca gat gct gtg gtg ctg ggt tca ccg ttg gct cgc gct 979 Ala Cys Gly Ala Asp Ala Val Val Leu Gly Ser Pro Leu Ala Arg Ala 280 285 290 gaa gaa gct gct gga aag ggc tac ttc tgg cca gca gtg gca gcg cac 1027 Glu Glu Ala Ala Gly Lys Gly Tyr Phe Trp Pro Ala Val Ala Ala His 295 300 305 cct cgt ttc cca cgc ggt gtg gtt act gag tcc gtg gac ttg gat gag 1075 Pro Arg Phe Pro Arg Gly Val Val Thr Glu Ser Val Asp Leu Asp Glu 310 315 320 325 gca gca cca agc ttg gag cag att ctg cat ggt ccg tct acg atg ccg 1123 Ala Ala Pro Ser Leu Glu Gln Ile Leu His Gly Pro Ser Thr Met Pro 330 335 340 tgg ggt gtg gaa aac ttc gaa ggt gga tta aag cgt gcg ctg gct aag 1171 Trp Gly Val Glu Asn Phe Glu Gly Gly Leu Lys Arg Ala Leu Ala Lys 345 350 355 tgt ggc tac acc gat ttg aag agc ttc caa aag gta agc ctg cac gtt 1219 Cys Gly Tyr Thr Asp Leu Lys Ser Phe Gln Lys Val Ser Leu His Val 360 365 370 aac taggtgtgtg tactcgcctc ttcgcgttgc 1252 Asn <210> 22 <211> 374 <212> PRT <213> Corynebacterium glutamicum <400> 22 Met Arg Asp His Val Glu Ile Gly Ile Gly Arg Glu Ala Arg Arg Thr 1 5 10 15 Tyr Ser Leu Asp Asp Ile Ser Val Val Ser Ser Arg Arg Thr Arg Ser 20 25 30 Ser Lys Asp Val Asp Thr Thr Trp His Ile Asp Ala Tyr Lys Phe Asp 35 40 45 Leu Pro Phe Met Asn His Pro Ser Asp Ala Leu Ala Ser Pro Glu Phe 50 55 60 Val Ile Glu Met Gly Lys Gln Gly Gly Leu Gly Val Ile Asn Ala Glu 65 70 75 80 Gly Leu Trp Gly Arg His Ala Asp Leu Asp Glu Ala Ile Ala Lys Val 85 90 95 Ile Ala Ala Tyr Glu Glu Gly Asp Gln Ala Ala Ala Thr Arg Thr Leu 100 105 110 Gln Glu Leu His Ala Ala Pro Leu Asp Thr Glu Leu Leu Ser Glu Arg 115 120 125 Ile Ala Gln Val Arg Asp Ser Gly Glu Ile Val Ala Val Arg Val Ser 130 135 140 Pro Gln Asn Val Arg Glu Ile Ala Pro Ile Val Ile Lys Ala Gly Ala 145 150 155 160 Asp Leu Leu Val Ile Gln Gly Thr Leu Ile Ser Ala Glu His Val Asn 165 170 175 Thr Gly Gly Glu Ala Leu Asn Leu Lys Glu Phe Ile Gly Ser Leu Asp 180 185 190 Val Pro Val Ile Ala Gly Gly Val Asn Asp Tyr Thr Thr Ala Leu His 195 200 205 Met Met Arg Thr Gly Ala Val Gly Ile Ile Val Gly Gly Gly Glu Asn 210 215 220 Thr Asn Ser Leu Ala Leu Gly Met Glu Val Ser Met Ala Thr Ala Ile 225 230 235 240 Ala Asp Val Ala Ala Ala Arg Arg Asp Tyr Leu Asp Glu Thr Gly Gly 245 250 255 Arg Tyr Val His Ile Ile Ala Asp Gly Ser Ile Glu Asn Ser Gly Asp 260 265 270 Val Val Lys Ala Ile Ala Cys Gly Ala Asp Ala Val Val Leu Gly Ser 275 280 285 Pro Leu Ala Arg Ala Glu Glu Ala Ala Gly Lys Gly Tyr Phe Trp Pro 290 295 300 Ala Val Ala Ala His Pro Arg Phe Pro Arg Gly Val Val Thr Glu Ser 305 310 315 320 Val Asp Leu Asp Glu Ala Ala Pro Ser Leu Glu Gln Ile Leu His Gly 325 330 335 Pro Ser Thr Met Pro Trp Gly Val Glu Asn Phe Glu Gly Gly Leu Lys 340 345 350 Arg Ala Leu Ala Lys Cys Gly Tyr Thr Asp Leu Lys Ser Phe Gln Lys 355 360 365 Val Ser Leu His Val Asn 370 <210> 23 <211> 1066 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1036) <223> RXA00552 <400> 23 ccgccaacaa ggcagcaaag ctcgatccaa ttgacgcctt gcgttatgag taaaagcctc 60 gtttttaagg tagccacaca tcgcactaga ctgaagaact gtg gct acc tca aaa 115 Val Ala Thr Ser Lys 1 5 att ctt ctt tat tac gca ttc acc ccg ctc tct gac cct aaa gcg gtt 163 Ile Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser Asp Pro Lys Ala Val 10 15 20 cag ctg tgg cag cgt gag ctc tgc gag tca ctg aat ctt cgt ggc cgc 211 Gln Leu Trp Gln Arg Glu Leu Cys Glu Ser Leu Asn Leu Arg Gly Arg 25 30 35 atc ctg atc tcc act cac ggc atc aat gga acc gtg ggc gga gat att 259 Ile Leu Ile Ser Thr His Gly Ile Asn Gly Thr Val Gly Gly Asp Ile 40 45 50 gat gat tgc aag gcg tac att aaa aag acc cgc gag tac cca ggt ttc 307 Asp Asp Cys Lys Ala Tyr Ile Lys Lys Thr Arg Glu Tyr Pro Gly Phe 55 60 65 aac cgc atg cag ttt aag tgg tcc gag ggt ggc gct gag gat ttc cca 355 Asn Arg Met Gln Phe Lys Trp Ser Glu Gly Gly Ala Glu Asp Phe Pro 70 75 80 85 aag ctc agt gtc aaa gtc cgc gat gag atc gtt gcc ttc ggc gct cca 403 Lys Leu Ser Val Lys Val Arg Asp Glu Ile Val Ala Phe Gly Ala Pro 90 95 100 gat gag ctc aaa gtg gat gaa aac ggc gtc gtc ggt ggc ggc gtt cac 451 Asp Glu Leu Lys Val Asp Glu Asn Gly Val Val Gly Gly Gly Val His 105 110 115 ctg aaa cca cag cag gtc aat gag ctt gtg gaa gcc cgt ggc gat gaa 499 Leu Lys Pro Gln Gln Val Asn Glu Leu Val Glu Ala Arg Gly Asp Glu 120 125 130 gtt gtg ttc ttt gac ggc cgc aac gca atg gaa gcc cag atc ggc aag 547 Val Val Phe Phe Asp Gly Arg Asn Ala Met Glu Ala Gln Ile Gly Lys 135 140 145 ttc aag gac gct gtt gtc cct gac gta gaa acc act cat gat ttc atc 595 Phe Lys Asp Ala Val Val Pro Asp Val Glu Thr Thr His Asp Phe Ile 150 155 160 165 gca gaa att gag tct gga aaa tac gac gat ctc aaa gac aag cct gtg 643 Ala Glu Ile Glu Ser Gly Lys Tyr Asp Asp Leu Lys Asp Lys Pro Val 170 175 180 gtc acc tac tgc acc ggc gga att cgt tgt gag atc ctg agt tca ctc 691 Val Thr Tyr Cys Thr Gly Gly Ile Arg Cys Glu Ile Leu Ser Ser Leu 185 190 195 atg atc aac cgt ggt ttc aaa gag gtc tac caa atc gat ggc ggc atc 739 Met Ile Asn Arg Gly Phe Lys Glu Val Tyr Gln Ile Asp Gly Gly Ile 200 205 210 gtt cgc tac ggc gag cag ttt ggc aac aag ggc ctg tgg gaa ggc tcc 787 Val Arg Tyr Gly Glu Gln Phe Gly Asn Lys Gly Leu Trp Glu Gly Ser 215 220 225 ctc tac gtt ttc gat aag cgc atg cat atg gaa ttc ggc gag gat tac 835 Leu Tyr Val Phe Asp Lys Arg Met His Met Glu Phe Gly Glu Asp Tyr 230 235 240 245 aaa gag gtc gga cac tgc atc cat tgc gat act ccc acc aac aaa ttt 883 Lys Glu Val Gly His Cys Ile His Cys Asp Thr Pro Thr Asn Lys Phe 250 255 260 gag cac tgc ctc aac gaa gat gat tgc cgc gag ctc gtg ttg atg tgc 931 Glu His Cys Leu Asn Glu Asp Asp Cys Arg Glu Leu Val Leu Met Cys 265 270 275 cct gat tgc ttc gcc aat gtt gag acc cgt cat tgc aag cgc gaa cgc 979 Pro Asp Cys Phe Ala Asn Val Glu Thr Arg His Cys Lys Arg Glu Arg 280 285 290 tgt gca gca att gct gcg gat ttc gct gag caa gga att gat ccg ctc 1027 Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu Gln Gly Ile Asp Pro Leu 295 300 305 gtt act tct taaaaagggt atggtggctg ggtatgagca 1066 Val Thr Ser 310 <210> 24 <211> 312 <212> PRT <213> Corynebacterium glutamicum <400> 24 Val Ala Thr Ser Lys Ile Leu Leu Tyr Tyr Ala Phe Thr Pro Leu Ser 1 5 10 15 Asp Pro Lys Ala Val Gln Leu Trp Gln Arg Glu Leu Cys Glu Ser Leu 20 25 30 Asn Leu Arg Gly Arg Ile Leu Ile Ser Thr His Gly Ile Asn Gly Thr 35 40 45 Val Gly Gly Asp Ile Asp Asp Cys Lys Ala Tyr Ile Lys Lys Thr Arg 50 55 60 Glu Tyr Pro Gly Phe Asn Arg Met Gln Phe Lys Trp Ser Glu Gly Gly 65 70 75 80 Ala Glu Asp Phe Pro Lys Leu Ser Val Lys Val Arg Asp Glu Ile Val 85 90 95 Ala Phe Gly Ala Pro Asp Glu Leu Lys Val Asp Glu Asn Gly Val Val 100 105 110 Gly Gly Gly Val His Leu Lys Pro Gln Gln Val Asn Glu Leu Val Glu 115 120 125 Ala Arg Gly Asp Glu Val Val Phe Phe Asp Gly Arg Asn Ala Met Glu 130 135 140 Ala Gln Ile Gly Lys Phe Lys Asp Ala Val Val Pro Asp Val Glu Thr 145 150 155 160 Thr His Asp Phe Ile Ala Glu Ile Glu Ser Gly Lys Tyr Asp Asp Leu 165 170 175 Lys Asp Lys Pro Val Val Thr Tyr Cys Thr Gly Gly Ile Arg Cys Glu 180 185 190 Ile Leu Ser Ser Leu Met Ile Asn Arg Gly Phe Lys Glu Val Tyr Gln 195 200 205 Ile Asp Gly Gly Ile Val Arg Tyr Gly Glu Gln Phe Gly Asn Lys Gly 210 215 220 Leu Trp Glu Gly Ser Leu Tyr Val Phe Asp Lys Arg Met His Met Glu 225 230 235 240 Phe Gly Glu Asp Tyr Lys Glu Val Gly His Cys Ile His Cys Asp Thr 245 250 255 Pro Thr Asn Lys Phe Glu His Cys Leu Asn Glu Asp Asp Cys Arg Glu 260 265 270 Leu Val Leu Met Cys Pro Asp Cys Phe Ala Asn Val Glu Thr Arg His 275 280 285 Cys Lys Arg Glu Arg Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu Gln 290 295 300 Gly Ile Asp Pro Leu Val Thr Ser 305 310 <210> 25 <211> 2005 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1975) <223> RXA00579 <400> 25 tggaagactg gaccatcgtc taagtttttc tttgagtttt catatgtaga aggcatcgtc 60 ggcttcggcc tggcggtgct tttctcgttg ttttgtggtt ttg tca gag gat gtc 115 Leu Ser Glu Asp Val 1 5 atg cgc gtt tta att att gat aat tat gat tct ttc acg ttt aat ctc 163 Met Arg Val Leu Ile Ile Asp Asn Tyr Asp Ser Phe Thr Phe Asn Leu 10 15 20 gcc acc tat gtg gaa gag gtt acg ggt cag gca cct gtg gtg gtg cct 211 Ala Thr Tyr Val Glu Glu Val Thr Gly Gln Ala Pro Val Val Val Pro 25 30 35 aat gat caa gaa ata gat gag atg ctt ttc gac gcc gtc atc ctc tca 259 Asn Asp Gln Glu Ile Asp Glu Met Leu Phe Asp Ala Val Ile Leu Ser 40 45 50 cct ggc ccg ggc cac gcc ggc gtt gcg gct gat ttt ggt atc tgt gca 307 Pro Gly Pro Gly His Ala Gly Val Ala Ala Asp Phe Gly Ile Cys Ala 55 60 65 ggc gtc att gag cgt gca cgc gtt ccg att ttg ggt gtg tgt tta ggc 355 Gly Val Ile Glu Arg Ala Arg Val Pro Ile Leu Gly Val Cys Leu Gly 70 75 80 85 cac cag ggc att gcg ttg gcc tat ggc ggt gat gtt gat ttg gcg ccc 403 His Gln Gly Ile Ala Leu Ala Tyr Gly Gly Asp Val Asp Leu Ala Pro 90 95 100 agg ccg gtc cac ggt gag gtt tcg cag atc acc cat gat ggt tca ggt 451 Arg Pro Val His Gly Glu Val Ser Gln Ile Thr His Asp Gly Ser Gly 105 110 115 tta ttt gca ggc atc cct gaa acg ttt gag gcg gtg cgt tat cac tcg 499 Leu Phe Ala Gly Ile Pro Glu Thr Phe Glu Ala Val Arg Tyr His Ser 120 125 130 atg gtg gca acc cgc ttg ccg gag tca ttg aaa gct aca gct acc agc 547 Met Val Ala Thr Arg Leu Pro Glu Ser Leu Lys Ala Thr Ala Thr Ser 135 140 145 gat gat ggt ttg atc atg gca ttg gca cat gaa gtg ctt ccg cag tgg 595 Asp Asp Gly Leu Ile Met Ala Leu Ala His Glu Val Leu Pro Gln Trp 150 155 160 165 ggt gtg caa ttt cat ccg gaa tct att ggt gga caa ttc ggc cat cag 643 Gly Val Gln Phe His Pro Glu Ser Ile Gly Gly Gln Phe Gly His Gln 170 175 180 atc att aag aac ttc ctt aat tta gcg cgc aca tat cgc tgg caa ctc 691 Ile Ile Lys Asn Phe Leu Asn Leu Ala Arg Thr Tyr Arg Trp Gln Leu 185 190 195 acg gag aaa act att ccg ctc agc gtt gat tca gca gcg gtt ttt gaa 739 Thr Glu Lys Thr Ile Pro Leu Ser Val Asp Ser Ala Ala Val Phe Glu 200 205 210 aca ttc ttt gcc cat tcc tcc cat gct ttt tgg ctc gat gat gcc caa 787 Thr Phe Phe Ala His Ser Ser His Ala Phe Trp Leu Asp Asp Ala Gln 215 220 225 gga acc agc tat ctt ggt gat gcc agc ggt cct ctc gca cgc aca aaa 835 Gly Thr Ser Tyr Leu Gly Asp Ala Ser Gly Pro Leu Ala Arg Thr Lys 230 235 240 245 acc cat aat gtc ggc gag ggg gat ttc ttc acc tgg cta aag gag gat 883 Thr His Asn Val Gly Glu Gly Asp Phe Phe Thr Trp Leu Lys Glu Asp 250 255 260 ctc gcc gcc aac tca gtt gcg ccc ggt caa ggt ttt cgt ctt ggc tgg 931 Leu Ala Ala Asn Ser Val Ala Pro Gly Gln Gly Phe Arg Leu Gly Trp 265 270 275 gtt ggt tac gtt ggt tat gag ctt aaa gcg gaa gct ggc gca cgg gct 979 Val Gly Tyr Val Gly Tyr Glu Leu Lys Ala Glu Ala Gly Ala Arg Ala 280 285 290 gcg cac act tcg agt ctt ccg gat gcg cac ctc att ttt gcc gat cgc 1027 Ala His Thr Ser Ser Leu Pro Asp Ala His Leu Ile Phe Ala Asp Arg 295 300 305 gcc atc gca gtg gaa tcg gat cag gtt cgg ttg ctg gcg ttg ggg gag 1075 Ala Ile Ala Val Glu Ser Asp Gln Val Arg Leu Leu Ala Leu Gly Glu 310 315 320 325 cag gac gag tgg ttt gaa gaa acc atc aag aag ctg cat aat ctt gtc 1123 Gln Asp Glu Trp Phe Glu Glu Thr Ile Lys Lys Leu His Asn Leu Val 330 335 340 gcc ccg cgg ata cct gcg tcc gga cac ctc gct ttg cag gtt cga gat 1171 Ala Pro Arg Ile Pro Ala Ser Gly His Leu Ala Leu Gln Val Arg Asp 345 350 355 tcc aaa gat gag tat ctc gac aaa att cgc aga gcc cag gag ctg att 1219 Ser Lys Asp Glu Tyr Leu Asp Lys Ile Arg Arg Ala Gln Glu Leu Ile 360 365 370 act cgc ggc gaa tcg tat gaa atc tgc ctg acc aca aaa ctt cag ggc 1267 Thr Arg Gly Glu Ser Tyr Glu Ile Cys Leu Thr Thr Lys Leu Gln Gly 375 380 385 acc act gat gtg gcc cct ctg gct gcc tat cta gca ctg cgt ggg gcc 1315 Thr Thr Asp Val Ala Pro Leu Ala Ala Tyr Leu Ala Leu Arg Gly Ala 390 395 400 405 aat ccc acc gca tat ggt gcg tat ctt cag ctg ggg gat acc tct att 1363 Asn Pro Thr Ala Tyr Gly Ala Tyr Leu Gln Leu Gly Asp Thr Ser Ile 410 415 420 ttg agt tcc tcg ccg gag cgg ttc atc acc att gat tcg gca ggg tat 1411 Leu Ser Ser Ser Pro Glu Arg Phe Ile Thr Ile Asp Ser Ala Gly Tyr 425 430 435 gtg gaa tca aag ccc att aaa ggc acc agg ccg cgt ggg cga aca gcg 1459 Val Glu Ser Lys Pro Ile Lys Gly Thr Arg Pro Arg Gly Arg Thr Ala 440 445 450 caa gaa gac caa gaa atc att gct gag ctg cgc agt aat cct aaa gat 1507 Gln Glu Asp Gln Glu Ile Ile Ala Glu Leu Arg Ser Asn Pro Lys Asp 455 460 465 cgt gca gaa aac ttg atg atc gtg gat ttg gtc cgc aac gac tta gcc 1555 Arg Ala Glu Asn Leu Met Ile Val Asp Leu Val Arg Asn Asp Leu Ala 470 475 480 485 cgc ggc gct ttg ccc acc aca gtt aaa aca tcc aag ctt ttc gac gtc 1603 Arg Gly Ala Leu Pro Thr Thr Val Lys Thr Ser Lys Leu Phe Asp Val 490 495 500 gaa acc tac gcc aca gtc cac caa ctt gtc agc acc gtc tct gca gag 1651 Glu Thr Tyr Ala Thr Val His Gln Leu Val Ser Thr Val Ser Ala Glu 505 510 515 ttg ggg cca cgc agt ccg att gag tgc gtg cgc gca gca ttc ccc ggt 1699 Leu Gly Pro Arg Ser Pro Ile Glu Cys Val Arg Ala Ala Phe Pro Gly 520 525 530 ggt tcg atg act ggt gcc cca aag ctg cgc acc atg gag atc atc gat 1747 Gly Ser Met Thr Gly Ala Pro Lys Leu Arg Thr Met Glu Ile Ile Asp 535 540 545 gag ctg gag gca gct cct cgc ggt att tac tca ggt ggc ttg gga tat 1795 Glu Leu Glu Ala Ala Pro Arg Gly Ile Tyr Ser Gly Gly Leu Gly Tyr 550 555 560 565 ttt tcc ctc gac ggc gca gtt gat ctc tcc atg gtg atc aga act ctc 1843 Phe Ser Leu Asp Gly Ala Val Asp Leu Ser Met Val Ile Arg Thr Leu 570 575 580 gtc atc cag aac aat cac gtg gag tac gga gtg ggc ggt gca ctt ctt 1891 Val Ile Gln Asn Asn His Val Glu Tyr Gly Val Gly Gly Ala Leu Leu 585 590 595 gct ctg tct gat ccg gag gct gag tgg gag gaa atc cgc gtt aaa tca 1939 Ala Leu Ser Asp Pro Glu Ala Glu Trp Glu Glu Ile Arg Val Lys Ser 600 605 610 cgg cct ctg ctg aat ttg ttt ggg gtt gaa ttc cca tgacgtacct 1985 Arg Pro Leu Leu Asn Leu Phe Gly Val Glu Phe Pro 615 620 625 cgtgtgggac ggtgcaacac 2005 <210> 26 <211> 625 <212> PRT <213> Corynebacterium glutamicum <400> 26 Leu Ser Glu Asp Val Met Arg Val Leu Ile Ile Asp Asn Tyr Asp Ser 1 5 10 15 Phe Thr Phe Asn Leu Ala Thr Tyr Val Glu Glu Val Thr Gly Gln Ala 20 25 30 Pro Val Val Val Pro Asn Asp Gln Glu Ile Asp Glu Met Leu Phe Asp 35 40 45 Ala Val Ile Leu Ser Pro Gly Pro Gly His Ala Gly Val Ala Ala Asp 50 55 60 Phe Gly Ile Cys Ala Gly Val Ile Glu Arg Ala Arg Val Pro Ile Leu 65 70 75 80 Gly Val Cys Leu Gly His Gln Gly Ile Ala Leu Ala Tyr Gly Gly Asp 85 90 95 Val Asp Leu Ala Pro Arg Pro Val His Gly Glu Val Ser Gln Ile Thr 100 105 110 His Asp Gly Ser Gly Leu Phe Ala Gly Ile Pro Glu Thr Phe Glu Ala 115 120 125 Val Arg Tyr His Ser Met Val Ala Thr Arg Leu Pro Glu Ser Leu Lys 130 135 140 Ala Thr Ala Thr Ser Asp Asp Gly Leu Ile Met Ala Leu Ala His Glu 145 150 155 160 Val Leu Pro Gln Trp Gly Val Gln Phe His Pro Glu Ser Ile Gly Gly 165 170 175 Gln Phe Gly His Gln Ile Ile Lys Asn Phe Leu Asn Leu Ala Arg Thr 180 185 190 Tyr Arg Trp Gln Leu Thr Glu Lys Thr Ile Pro Leu Ser Val Asp Ser 195 200 205 Ala Ala Val Phe Glu Thr Phe Phe Ala His Ser Ser His Ala Phe Trp 210 215 220 Leu Asp Asp Ala Gln Gly Thr Ser Tyr Leu Gly Asp Ala Ser Gly Pro 225 230 235 240 Leu Ala Arg Thr Lys Thr His Asn Val Gly Glu Gly Asp Phe Phe Thr 245 250 255 Trp Leu Lys Glu Asp Leu Ala Ala Asn Ser Val Ala Pro Gly Gln Gly 260 265 270 Phe Arg Leu Gly Trp Val Gly Tyr Val Gly Tyr Glu Leu Lys Ala Glu 275 280 285 Ala Gly Ala Arg Ala Ala His Thr Ser Ser Leu Pro Asp Ala His Leu 290 295 300 Ile Phe Ala Asp Arg Ala Ile Ala Val Glu Ser Asp Gln Val Arg Leu 305 310 315 320 Leu Ala Leu Gly Glu Gln Asp Glu Trp Phe Glu Glu Thr Ile Lys Lys 325 330 335 Leu His Asn Leu Val Ala Pro Arg Ile Pro Ala Ser Gly His Leu Ala 340 345 350 Leu Gln Val Arg Asp Ser Lys Asp Glu Tyr Leu Asp Lys Ile Arg Arg 355 360 365 Ala Gln Glu Leu Ile Thr Arg Gly Glu Ser Tyr Glu Ile Cys Leu Thr 370 375 380 Thr Lys Leu Gln Gly Thr Thr Asp Val Ala Pro Leu Ala Ala Tyr Leu 385 390 395 400 Ala Leu Arg Gly Ala Asn Pro Thr Ala Tyr Gly Ala Tyr Leu Gln Leu 405 410 415 Gly Asp Thr Ser Ile Leu Ser Ser Ser Pro Glu Arg Phe Ile Thr Ile 420 425 430 Asp Ser Ala Gly Tyr Val Glu Ser Lys Pro Ile Lys Gly Thr Arg Pro 435 440 445 Arg Gly Arg Thr Ala Gln Glu Asp Gln Glu Ile Ile Ala Glu Leu Arg 450 455 460 Ser Asn Pro Lys Asp Arg Ala Glu Asn Leu Met Ile Val Asp Leu Val 465 470 475 480 Arg Asn Asp Leu Ala Arg Gly Ala Leu Pro Thr Thr Val Lys Thr Ser 485 490 495 Lys Leu Phe Asp Val Glu Thr Tyr Ala Thr Val His Gln Leu Val Ser 500 505 510 Thr Val Ser Ala Glu Leu Gly Pro Arg Ser Pro Ile Glu Cys Val Arg 515 520 525 Ala Ala Phe Pro Gly Gly Ser Met Thr Gly Ala Pro Lys Leu Arg Thr 530 535 540 Met Glu Ile Ile Asp Glu Leu Glu Ala Ala Pro Arg Gly Ile Tyr Ser 545 550 555 560 Gly Gly Leu Gly Tyr Phe Ser Leu Asp Gly Ala Val Asp Leu Ser Met 565 570 575 Val Ile Arg Thr Leu Val Ile Gln Asn Asn His Val Glu Tyr Gly Val 580 585 590 Gly Gly Ala Leu Leu Ala Leu Ser Asp Pro Glu Ala Glu Trp Glu Glu 595 600 605 Ile Arg Val Lys Ser Arg Pro Leu Leu Asn Leu Phe Gly Val Glu Phe 610 615 620 Pro 625 <210> 27 <211> 1120 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1090) <223> RXA00620 <400> 27 gcttattagc tgtacggctc caaggttctc ttaatctttg ttcgaataaa ctgccagctt 60 aaaaatgtct tttaggtaaa caaaaaattc gggcgaatgg gtg atc cat gtc agg 115 Val Ile His Val Arg 1 5 aag cca gcg gtg aaa ccg gca gtg aaa cca gcg gtg aat gct aaa att 163 Lys Pro Ala Val Lys Pro Ala Val Lys Pro Ala Val Asn Ala Lys Ile 10 15 20 ttc cga aca cac ccg agg ggt cta gac ttg cct aac atg cgt cct gaa 211 Phe Arg Thr His Pro Arg Gly Leu Asp Leu Pro Asn Met Arg Pro Glu 25 30 35 ctc tcc cag tac aag cac ctg tcg gca ggc aag gtc cgt gag atc tac 259 Leu Ser Gln Tyr Lys His Leu Ser Ala Gly Lys Val Arg Glu Ile Tyr 40 45 50 gag atc gac gac aag cac atc ctc atg gtg gct tcc gat cgt atc tct 307 Glu Ile Asp Asp Lys His Ile Leu Met Val Ala Ser Asp Arg Ile Ser 55 60 65 gca tac gat ttc atc ctc gat acc gaa att cca gac aag ggt cga gtg 355 Ala Tyr Asp Phe Ile Leu Asp Thr Glu Ile Pro Asp Lys Gly Arg Val 70 75 80 85 ctc act gcg atg agc cag ttc ttc ttc gac acc atc gat ttt cct aat 403 Leu Thr Ala Met Ser Gln Phe Phe Phe Asp Thr Ile Asp Phe Pro Asn 90 95 100 cac ctt gca ggt ccc gct gat gat cca cgt atc cca gaa gaa gtt ttg 451 His Leu Ala Gly Pro Ala Asp Asp Pro Arg Ile Pro Glu Glu Val Leu 105 110 115 gga cga gca atg gtg tgc aag aag ctc aac atg ctt cct ttt gaa tgc 499 Gly Arg Ala Met Val Cys Lys Lys Leu Asn Met Leu Pro Phe Glu Cys 120 125 130 gtg gtt cgt gga tac ctc act ggc tct gga ctt gtt gaa tac aag cag 547 Val Val Arg Gly Tyr Leu Thr Gly Ser Gly Leu Val Glu Tyr Lys Gln 135 140 145 acc agc tcc gtg tgt gga gtt gag ctc cca gaa ggc ctc gtt gaa tct 595 Thr Ser Ser Val Cys Gly Val Glu Leu Pro Glu Gly Leu Val Glu Ser 150 155 160 165 tct cag ctg cct gag cca atc ttt acc cca gcc acc aag gct gac atc 643 Ser Gln Leu Pro Glu Pro Ile Phe Thr Pro Ala Thr Lys Ala Asp Ile 170 175 180 ggc gac cac gac atc aat gtc tcc ttt gac gtt gtt gaa gaa cgt ctc 691 Gly Asp His Asp Ile Asn Val Ser Phe Asp Val Val Glu Glu Arg Leu 185 190 195 ggc gaa gct cgt gcg aac cag ttg cgc gat gcc tct att gct att tac 739 Gly Glu Ala Arg Ala Asn Gln Leu Arg Asp Ala Ser Ile Ala Ile Tyr 200 205 210 aag gct gct gct gag atc gcc cgt gac cgt ggc gtc atc ctt gcc gac 787 Lys Ala Ala Ala Glu Ile Ala Arg Asp Arg Gly Val Ile Leu Ala Asp 215 220 225 acc aaa ttt gag ttc ggc atc gat gaa gat ggc acc ctc gtg ctt ggt 835 Thr Lys Phe Glu Phe Gly Ile Asp Glu Asp Gly Thr Leu Val Leu Gly 230 235 240 245 gat gaa gtc ctt acc cca gat tcc tcc cgc tac tgg cct ttg gaa ggc 883 Asp Glu Val Leu Thr Pro Asp Ser Ser Arg Tyr Trp Pro Leu Glu Gly 250 255 260 tat gaa gca gga tct gtg caa cca agc ttt gat aag caa ttc gtg cgc 931 Tyr Glu Ala Gly Ser Val Gln Pro Ser Phe Asp Lys Gln Phe Val Arg 265 270 275 aac tgg ctc acc ggc cct aaa tct ggc tgg gac aag gat tcc ggc ttg 979 Asn Trp Leu Thr Gly Pro Lys Ser Gly Trp Asp Lys Asp Ser Gly Leu 280 285 290 gag cca cca gct ctg cca ggt tcc gtt gtt gag gca acc cgc gag cgc 1027 Glu Pro Pro Ala Leu Pro Gly Ser Val Val Glu Ala Thr Arg Glu Arg 295 300 305 tac atc gag gcc tac gag ctg att tct ggt cag aag ttc tgc cag tgg 1075 Tyr Ile Glu Ala Tyr Glu Leu Ile Ser Gly Gln Lys Phe Cys Gln Trp 310 315 320 325 att ggt tct tgc gtc taagctgctt gattttccct aaaccccgga 1120 Ile Gly Ser Cys Val 330 <210> 28 <211> 330 <212> PRT <213> Corynebacterium glutamicum <400> 28 Val Ile His Val Arg Lys Pro Ala Val Lys Pro Ala Val Lys Pro Ala 1 5 10 15 Val Asn Ala Lys Ile Phe Arg Thr His Pro Arg Gly Leu Asp Leu Pro 20 25 30 Asn Met Arg Pro Glu Leu Ser Gln Tyr Lys His Leu Ser Ala Gly Lys 35 40 45 Val Arg Glu Ile Tyr Glu Ile Asp Asp Lys His Ile Leu Met Val Ala 50 55 60 Ser Asp Arg Ile Ser Ala Tyr Asp Phe Ile Leu Asp Thr Glu Ile Pro 65 70 75 80 Asp Lys Gly Arg Val Leu Thr Ala Met Ser Gln Phe Phe Phe Asp Thr 85 90 95 Ile Asp Phe Pro Asn His Leu Ala Gly Pro Ala Asp Asp Pro Arg Ile 100 105 110 Pro Glu Glu Val Leu Gly Arg Ala Met Val Cys Lys Lys Leu Asn Met 115 120 125 Leu Pro Phe Glu Cys Val Val Arg Gly Tyr Leu Thr Gly Ser Gly Leu 130 135 140 Val Glu Tyr Lys Gln Thr Ser Ser Val Cys Gly Val Glu Leu Pro Glu 145 150 155 160 Gly Leu Val Glu Ser Ser Gln Leu Pro Glu Pro Ile Phe Thr Pro Ala 165 170 175 Thr Lys Ala Asp Ile Gly Asp His Asp Ile Asn Val Ser Phe Asp Val 180 185 190 Val Glu Glu Arg Leu Gly Glu Ala Arg Ala Asn Gln Leu Arg Asp Ala 195 200 205 Ser Ile Ala Ile Tyr Lys Ala Ala Ala Glu Ile Ala Arg Asp Arg Gly 210 215 220 Val Ile Leu Ala Asp Thr Lys Phe Glu Phe Gly Ile Asp Glu Asp Gly 225 230 235 240 Thr Leu Val Leu Gly Asp Glu Val Leu Thr Pro Asp Ser Ser Arg Tyr 245 250 255 Trp Pro Leu Glu Gly Tyr Glu Ala Gly Ser Val Gln Pro Ser Phe Asp 260 265 270 Lys Gln Phe Val Arg Asn Trp Leu Thr Gly Pro Lys Ser Gly Trp Asp 275 280 285 Lys Asp Ser Gly Leu Glu Pro Pro Ala Leu Pro Gly Ser Val Val Glu 290 295 300 Ala Thr Arg Glu Arg Tyr Ile Glu Ala Tyr Glu Leu Ile Ser Gly Gln 305 310 315 320 Lys Phe Cys Gln Trp Ile Gly Ser Cys Val 325 330 <210> 29 <211> 1159 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1129) <223> RXA00636 <400> 29 tcatgggatt cagcaaggcg gccacccgaa ccgtctttgg tggaggagta ggagcgatga 60 tcgatctggc ccgttcgaac ataaggaata ttcctactcc atg atg att gat aca 115 Met Met Ile Asp Thr 1 5 cct gct gtt ctc att gac cgc gag cgc tta act gcc aac att tcc agg 163 Pro Ala Val Leu Ile Asp Arg Glu Arg Leu Thr Ala Asn Ile Ser Arg 10 15 20 atg gca gct cac gcc ggt gcc cat gag att gcc ctg cgt ccg cat gtg 211 Met Ala Ala His Ala Gly Ala His Glu Ile Ala Leu Arg Pro His Val 25 30 35 aaa acg cac aaa atc att gaa att gcg cag atg cag gtc gac gcc ggt 259 Lys Thr His Lys Ile Ile Glu Ile Ala Gln Met Gln Val Asp Ala Gly 40 45 50 gcc cga ggg atc acc tgc gca acc att ggc gag gcg gaa att ttt gcc 307 Ala Arg Gly Ile Thr Cys Ala Thr Ile Gly Glu Ala Glu Ile Phe Ala 55 60 65 ggc gca ggt ttt acg gac atc ttt att gca tat ccg ctg tat cta acc 355 Gly Ala Gly Phe Thr Asp Ile Phe Ile Ala Tyr Pro Leu Tyr Leu Thr 70 75 80 85 gat cat gca gtg caa cgc ctg aac gcg atc ccc gga gaa att tcc att 403 Asp His Ala Val Gln Arg Leu Asn Ala Ile Pro Gly Glu Ile Ser Ile 90 95 100 ggc gtg gat tcg gta gag atg gca cag gcg acg gcg ggt ttg cgg gaa 451 Gly Val Asp Ser Val Glu Met Ala Gln Ala Thr Ala Gly Leu Arg Glu 105 110 115 gat atc aag gct ctg att gaa gtg gat tcg gga cat cgt aga agt gga 499 Asp Ile Lys Ala Leu Ile Glu Val Asp Ser Gly His Arg Arg Ser Gly 120 125 130 gtc acg gcg act gct tca gaa ttg agt cag atc cgc gag gcg ctg ggc 547 Val Thr Ala Thr Ala Ser Glu Leu Ser Gln Ile Arg Glu Ala Leu Gly 135 140 145 agc agg tat gca gga gtg ttt act ttt cct ggg cat tct tat ggc ccg 595 Ser Arg Tyr Ala Gly Val Phe Thr Phe Pro Gly His Ser Tyr Gly Pro 150 155 160 165 gga aat ggt gag cag gca gca gct gat gag ctt cag gct cta aac aac 643 Gly Asn Gly Glu Gln Ala Ala Ala Asp Glu Leu Gln Ala Leu Asn Asn 170 175 180 agc gtc cag cga ctt gct ggc ggc ctg act tct ggc ggt tcc tcg ccg 691 Ser Val Gln Arg Leu Ala Gly Gly Leu Thr Ser Gly Gly Ser Ser Pro 185 190 195 tct gcg cag ttt aca gac gca atc gat gag atg cga cca ggc gtg tat 739 Ser Ala Gln Phe Thr Asp Ala Ile Asp Glu Met Arg Pro Gly Val Tyr 200 205 210 gtg ttt aac gat tcc cag cag atc acc tcg gga gca tgc act gag aag 787 Val Phe Asn Asp Ser Gln Gln Ile Thr Ser Gly Ala Cys Thr Glu Lys 215 220 225 cag gtg gca atg acg gtg ctg tct act gtg gtc agc cga aat gtg tca 835 Gln Val Ala Met Thr Val Leu Ser Thr Val Val Ser Arg Asn Val Ser 230 235 240 245 gat cgt cgg atc att ttg gat gcg gga tcc aaa atc ctc agc act gat 883 Asp Arg Arg Ile Ile Leu Asp Ala Gly Ser Lys Ile Leu Ser Thr Asp 250 255 260 aaa cca gca tgg att gat ggc aat ggt ttt gtt ctg ggg aat cct gaa 931 Lys Pro Ala Trp Ile Asp Gly Asn Gly Phe Val Leu Gly Asn Pro Glu 265 270 275 gcc cga atc tct gct ttg tcg gag cat cac gca acc att ttc tgg cca 979 Ala Arg Ile Ser Ala Leu Ser Glu His His Ala Thr Ile Phe Trp Pro 280 285 290 gat aaa gtg cta ctt cca gta atc ggg gag cag ctc aac atc gtg ccc 1027 Asp Lys Val Leu Leu Pro Val Ile Gly Glu Gln Leu Asn Ile Val Pro 295 300 305 aac cat gcc tgc aac gtg att aat ttg gtg gat gag gtc tac gtt cgg 1075 Asn His Ala Cys Asn Val Ile Asn Leu Val Asp Glu Val Tyr Val Arg 310 315 320 325 gaa gcc gat ggc act ttc cgt acc tgg aag gta gtt gcc cgc ggc aga 1123 Glu Ala Asp Gly Thr Phe Arg Thr Trp Lys Val Val Ala Arg Gly Arg 330 335 340 aac aat tagggaaacc tcttgacctt cacgcgacgt 1159 Asn Asn <210> 30 <211> 343 <212> PRT <213> Corynebacterium glutamicum <400> 30 Met Met Ile Asp Thr Pro Ala Val Leu Ile Asp Arg Glu Arg Leu Thr 1 5 10 15 Ala Asn Ile Ser Arg Met Ala Ala His Ala Gly Ala His Glu Ile Ala 20 25 30 Leu Arg Pro His Val Lys Thr His Lys Ile Ile Glu Ile Ala Gln Met 35 40 45 Gln Val Asp Ala Gly Ala Arg Gly Ile Thr Cys Ala Thr Ile Gly Glu 50 55 60 Ala Glu Ile Phe Ala Gly Ala Gly Phe Thr Asp Ile Phe Ile Ala Tyr 65 70 75 80 Pro Leu Tyr Leu Thr Asp His Ala Val Gln Arg Leu Asn Ala Ile Pro 85 90 95 Gly Glu Ile Ser Ile Gly Val Asp Ser Val Glu Met Ala Gln Ala Thr 100 105 110 Ala Gly Leu Arg Glu Asp Ile Lys Ala Leu Ile Glu Val Asp Ser Gly 115 120 125 His Arg Arg Ser Gly Val Thr Ala Thr Ala Ser Glu Leu Ser Gln Ile 130 135 140 Arg Glu Ala Leu Gly Ser Arg Tyr Ala Gly Val Phe Thr Phe Pro Gly 145 150 155 160 His Ser Tyr Gly Pro Gly Asn Gly Glu Gln Ala Ala Ala Asp Glu Leu 165 170 175 Gln Ala Leu Asn Asn Ser Val Gln Arg Leu Ala Gly Gly Leu Thr Ser 180 185 190 Gly Gly Ser Ser Pro Ser Ala Gln Phe Thr Asp Ala Ile Asp Glu Met 195 200 205 Arg Pro Gly Val Tyr Val Phe Asn Asp Ser Gln Gln Ile Thr Ser Gly 210 215 220 Ala Cys Thr Glu Lys Gln Val Ala Met Thr Val Leu Ser Thr Val Val 225 230 235 240 Ser Arg Asn Val Ser Asp Arg Arg Ile Ile Leu Asp Ala Gly Ser Lys 245 250 255 Ile Leu Ser Thr Asp Lys Pro Ala Trp Ile Asp Gly Asn Gly Phe Val 260 265 270 Leu Gly Asn Pro Glu Ala Arg Ile Ser Ala Leu Ser Glu His His Ala 275 280 285 Thr Ile Phe Trp Pro Asp Lys Val Leu Leu Pro Val Ile Gly Glu Gln 290 295 300 Leu Asn Ile Val Pro Asn His Ala Cys Asn Val Ile Asn Leu Val Asp 305 310 315 320 Glu Val Tyr Val Arg Glu Ala Asp Gly Thr Phe Arg Thr Trp Lys Val 325 330 335 Val Ala Arg Gly Arg Asn Asn 340 <210> 31 <211> 937 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(907) <223> RXA00708 <400> 31 cctgcgtatc ggctgccttt ttgaattctt ttcctcctcg aggcctaacc ttcaattcct 60 taccgatccc cttccctgaa gtttcgctaa cctggcgtac atg act ctt tcc ctt 115 Met Thr Leu Ser Leu 1 5 cct cca att ggt ttc ggc acc gtt cat ctt gat ggc gca cct ggc gtt 163 Pro Pro Ile Gly Phe Gly Thr Val His Leu Asp Gly Ala Pro Gly Val 10 15 20 gaa gcc atc gct act gcc att gat gct ggt tac cgc ctc atc gac acc 211 Glu Ala Ile Ala Thr Ala Ile Asp Ala Gly Tyr Arg Leu Ile Asp Thr 25 30 35 gcg tac aac tat gaa aat gaa ggt acc gtg ggc aag gct gtc cgc gag 259 Ala Tyr Asn Tyr Glu Asn Glu Gly Thr Val Gly Lys Ala Val Arg Glu 40 45 50 tcg ggt gtc ccc cgc gag gaa ttg att gtt acc agt aag ctc cct ggc 307 Ser Gly Val Pro Arg Glu Glu Leu Ile Val Thr Ser Lys Leu Pro Gly 55 60 65 cgc ttc cat gct cgc gat cta gga cgc gtc cgc att gag gaa agt cta 355 Arg Phe His Ala Arg Asp Leu Gly Arg Val Arg Ile Glu Glu Ser Leu 70 75 80 85 tac cgc ctc aac tta gat tac atc gat ctc ctc ttg att cac tgg cct 403 Tyr Arg Leu Asn Leu Asp Tyr Ile Asp Leu Leu Leu Ile His Trp Pro 90 95 100 aat ccc agc aag gat ctc tac gtc gag gcg tgg gaa acg ctg att gaa 451 Asn Pro Ser Lys Asp Leu Tyr Val Glu Ala Trp Glu Thr Leu Ile Glu 105 110 115 gtc cgc gat gct ggc ctg gtc aag cac atc gga gtg tct aac ttc ctt 499 Val Arg Asp Ala Gly Leu Val Lys His Ile Gly Val Ser Asn Phe Leu 120 125 130 cca aat cac att gat cgc ctg cgc cgc gaa acc ggt gaa ctg ccg gcc 547 Pro Asn His Ile Asp Arg Leu Arg Arg Glu Thr Gly Glu Leu Pro Ala 135 140 145 gtt aac cag atc gag ttg cac ccc tat ttc ccg cag gtg gag cag gta 595 Val Asn Gln Ile Glu Leu His Pro Tyr Phe Pro Gln Val Glu Gln Val 150 155 160 165 gat ttc cac gat gag ctg ggc atc att acc gag gcc tgg agc ccg ctc 643 Asp Phe His Asp Glu Leu Gly Ile Ile Thr Glu Ala Trp Ser Pro Leu 170 175 180 agc aac ggt cgc gga ctc gtc gaa gag cca ttg ctc aag gaa atc ggc 691 Ser Asn Gly Arg Gly Leu Val Glu Glu Pro Leu Leu Lys Glu Ile Gly 185 190 195 gag cgc tac ggg gtc ggc agc ggc gaa atc gcc ctc gct tgg cat cac 739 Glu Arg Tyr Gly Val Gly Ser Gly Glu Ile Ala Leu Ala Trp His His 200 205 210 gcc agg gga atc gtt ccg att cca cgc tcc acc aac ccg gcc agg cag 787 Ala Arg Gly Ile Val Pro Ile Pro Arg Ser Thr Asn Pro Ala Arg Gln 215 220 225 cgc agc aac ttg gag gcg gta aag att tcg ctt atc gac gaa gac gtc 835 Arg Ser Asn Leu Glu Ala Val Lys Ile Ser Leu Ile Asp Glu Asp Val 230 235 240 245 cag gcg att acc gct ttg gcg cgc aaa aac ggc cgg atc aaa gat caa 883 Gln Ala Ile Thr Ala Leu Ala Arg Lys Asn Gly Arg Ile Lys Asp Gln 250 255 260 gat cca gcc gtc tat gaa gaa ttc tagatagtta catcaaggtt ccgaggaggc 937 Asp Pro Ala Val Tyr Glu Glu Phe 265 <210> 32 <211> 269 <212> PRT <213> Corynebacterium glutamicum <400> 32 Met Thr Leu Ser Leu Pro Pro Ile Gly Phe Gly Thr Val His Leu Asp 1 5 10 15 Gly Ala Pro Gly Val Glu Ala Ile Ala Thr Ala Ile Asp Ala Gly Tyr 20 25 30 Arg Leu Ile Asp Thr Ala Tyr Asn Tyr Glu Asn Glu Gly Thr Val Gly 35 40 45 Lys Ala Val Arg Glu Ser Gly Val Pro Arg Glu Glu Leu Ile Val Thr 50 55 60 Ser Lys Leu Pro Gly Arg Phe His Ala Arg Asp Leu Gly Arg Val Arg 65 70 75 80 Ile Glu Glu Ser Leu Tyr Arg Leu Asn Leu Asp Tyr Ile Asp Leu Leu 85 90 95 Leu Ile His Trp Pro Asn Pro Ser Lys Asp Leu Tyr Val Glu Ala Trp 100 105 110 Glu Thr Leu Ile Glu Val Arg Asp Ala Gly Leu Val Lys His Ile Gly 115 120 125 Val Ser Asn Phe Leu Pro Asn His Ile Asp Arg Leu Arg Arg Glu Thr 130 135 140 Gly Glu Leu Pro Ala Val Asn Gln Ile Glu Leu His Pro Tyr Phe Pro 145 150 155 160 Gln Val Glu Gln Val Asp Phe His Asp Glu Leu Gly Ile Ile Thr Glu 165 170 175 Ala Trp Ser Pro Leu Ser Asn Gly Arg Gly Leu Val Glu Glu Pro Leu 180 185 190 Leu Lys Glu Ile Gly Glu Arg Tyr Gly Val Gly Ser Gly Glu Ile Ala 195 200 205 Leu Ala Trp His His Ala Arg Gly Ile Val Pro Ile Pro Arg Ser Thr 210 215 220 Asn Pro Ala Arg Gln Arg Ser Asn Leu Glu Ala Val Lys Ile Ser Leu 225 230 235 240 Ile Asp Glu Asp Val Gln Ala Ile Thr Ala Leu Ala Arg Lys Asn Gly 245 250 255 Arg Ile Lys Asp Gln Asp Pro Ala Val Tyr Glu Glu Phe 260 265 <210> 33 <211> 1132 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1102) <223> RXA00727 <400> 33 tagctgttta ttttaaagtc cctcccgagc ccatgccgga taaaacaaaa cccgagcgcc 60 tgccaacgca aaatccagcc tcacttaagg ttattgcacc atg gca ggt agt att 115 Met Ala Gly Ser Ile 1 5 ttg ccc atg att gag agt ttc acc cga ttg cga att tcg cag aca ccg 163 Leu Pro Met Ile Glu Ser Phe Thr Arg Leu Arg Ile Ser Gln Thr Pro 10 15 20 cct ctg aaa cgg cta ctg cat agc act ttg cgt ttc ccc aaa atc ccc 211 Pro Leu Lys Arg Leu Leu His Ser Thr Leu Arg Phe Pro Lys Ile Pro 25 30 35 aag cgc gct gta gcg gcg acc gtc ggc atc gtg gca acc tca ttc acc 259 Lys Arg Ala Val Ala Ala Thr Val Gly Ile Val Ala Thr Ser Phe Thr 40 45 50 ttg gct tct tgt gtc acc aat gag gag cag ggc aac cca gat ggc tgg 307 Leu Ala Ser Cys Val Thr Asn Glu Glu Gln Gly Asn Pro Asp Gly Trp 55 60 65 gag cag atc gtt cca gat cct gta ccg gag att cag gcg atg gtt ccc 355 Glu Gln Ile Val Pro Asp Pro Val Pro Glu Ile Gln Ala Met Val Pro 70 75 80 85 gaa gct ctg gct cag cgc ggt gtg ctc acc gcc ggt gcc aac cca cct 403 Glu Ala Leu Ala Gln Arg Gly Val Leu Thr Ala Gly Ala Asn Pro Pro 90 95 100 ttc cca ccg ttt gag ttt aaa gat tcc gat ggt cag att atc ggt gtg 451 Phe Pro Pro Phe Glu Phe Lys Asp Ser Asp Gly Gln Ile Ile Gly Val 105 110 115 gaa atg gac ctc gtg cgt gca atg gcg ggg gtg atg ggc ttg gag ttc 499 Glu Met Asp Leu Val Arg Ala Met Ala Gly Val Met Gly Leu Glu Phe 120 125 130 agc cct cag gag cag gat ttc tcc ctc atc ctt cca tcg gtt caa gct 547 Ser Pro Gln Glu Gln Asp Phe Ser Leu Ile Leu Pro Ser Val Gln Ala 135 140 145 ggc acc ctt gat atc gga gcc tct ggc ttc act gac aac gag gaa cgc 595 Gly Thr Leu Asp Ile Gly Ala Ser Gly Phe Thr Asp Asn Glu Glu Arg 150 155 160 165 cgc gag aac ttt gat ttc atc gat ttc ctc ttc gca ggt gtg cag tgg 643 Arg Glu Asn Phe Asp Phe Ile Asp Phe Leu Phe Ala Gly Val Gln Trp 170 175 180 gcg cag gca act gat cgt gaa acc cca atc gat ccg gaa aac gcc tgt 691 Ala Gln Ala Thr Asp Arg Glu Thr Pro Ile Asp Pro Glu Asn Ala Cys 185 190 195 ggt ctc acc gtt gct gta cag cgc aca acc gtg gca gag acc gac gat 739 Gly Leu Thr Val Ala Val Gln Arg Thr Thr Val Ala Glu Thr Asp Asp 200 205 210 gtc cgt cct cgc tca gct caa tgt gaa gcc gaa ggc aaa gag ccg atc 787 Val Arg Pro Arg Ser Ala Gln Cys Glu Ala Glu Gly Lys Glu Pro Ile 215 220 225 acc att ttg tct tat gaa acc gca gat act gca gct acc gca ttg atc 835 Thr Ile Leu Ser Tyr Glu Thr Ala Asp Thr Ala Ala Thr Ala Leu Ile 230 235 240 245 ctg gga cgc gca gac gca ctt gct gcg gac tcc cct gtt tca gct tgg 883 Leu Gly Arg Ala Asp Ala Leu Ala Ala Asp Ser Pro Val Ser Ala Trp 250 255 260 gct gca gag cgc tcc gaa ggc cgc atc gaa gtt gtg ggc gat atg tac 931 Ala Ala Glu Arg Ser Glu Gly Arg Ile Glu Val Val Gly Asp Met Tyr 265 270 275 ttg gct gcg cca ttt ggt ttc gca ttc ccg ttg gaa tct gac ctc acc 979 Leu Ala Ala Pro Phe Gly Phe Ala Phe Pro Leu Glu Ser Asp Leu Thr 280 285 290 cca gca gca gct gcg gcg ttc caa cac ttg att gac acc ggc gat tac 1027 Pro Ala Ala Ala Ala Ala Phe Gln His Leu Ile Asp Thr Gly Asp Tyr 295 300 305 cag cgc atc atg gcg caa tgg ggc att gaa gaa ggc ctt ctt gat gag 1075 Gln Arg Ile Met Ala Gln Trp Gly Ile Glu Glu Gly Leu Leu Asp Glu 310 315 320 325 gcc ctg atc aac gaa cag cca ctc aac tagagccttc cagcaactaa 1122 Ala Leu Ile Asn Glu Gln Pro Leu Asn 330 aaacaattaa 1132 <210> 34 <211> 334 <212> PRT <213> Corynebacterium glutamicum <400> 34 Met Ala Gly Ser Ile Leu Pro Met Ile Glu Ser Phe Thr Arg Leu Arg 1 5 10 15 Ile Ser Gln Thr Pro Pro Leu Lys Arg Leu Leu His Ser Thr Leu Arg 20 25 30 Phe Pro Lys Ile Pro Lys Arg Ala Val Ala Ala Thr Val Gly Ile Val 35 40 45 Ala Thr Ser Phe Thr Leu Ala Ser Cys Val Thr Asn Glu Glu Gln Gly 50 55 60 Asn Pro Asp Gly Trp Glu Gln Ile Val Pro Asp Pro Val Pro Glu Ile 65 70 75 80 Gln Ala Met Val Pro Glu Ala Leu Ala Gln Arg Gly Val Leu Thr Ala 85 90 95 Gly Ala Asn Pro Pro Phe Pro Pro Phe Glu Phe Lys Asp Ser Asp Gly 100 105 110 Gln Ile Ile Gly Val Glu Met Asp Leu Val Arg Ala Met Ala Gly Val 115 120 125 Met Gly Leu Glu Phe Ser Pro Gln Glu Gln Asp Phe Ser Leu Ile Leu 130 135 140 Pro Ser Val Gln Ala Gly Thr Leu Asp Ile Gly Ala Ser Gly Phe Thr 145 150 155 160 Asp Asn Glu Glu Arg Arg Glu Asn Phe Asp Phe Ile Asp Phe Leu Phe 165 170 175 Ala Gly Val Gln Trp Ala Gln Ala Thr Asp Arg Glu Thr Pro Ile Asp 180 185 190 Pro Glu Asn Ala Cys Gly Leu Thr Val Ala Val Gln Arg Thr Thr Val 195 200 205 Ala Glu Thr Asp Asp Val Arg Pro Arg Ser Ala Gln Cys Glu Ala Glu 210 215 220 Gly Lys Glu Pro Ile Thr Ile Leu Ser Tyr Glu Thr Ala Asp Thr Ala 225 230 235 240 Ala Thr Ala Leu Ile Leu Gly Arg Ala Asp Ala Leu Ala Ala Asp Ser 245 250 255 Pro Val Ser Ala Trp Ala Ala Glu Arg Ser Glu Gly Arg Ile Glu Val 260 265 270 Val Gly Asp Met Tyr Leu Ala Ala Pro Phe Gly Phe Ala Phe Pro Leu 275 280 285 Glu Ser Asp Leu Thr Pro Ala Ala Ala Ala Ala Phe Gln His Leu Ile 290 295 300 Asp Thr Gly Asp Tyr Gln Arg Ile Met Ala Gln Trp Gly Ile Glu Glu 305 310 315 320 Gly Leu Leu Asp Glu Ala Leu Ile Asn Glu Gln Pro Leu Asn 325 330 <210> 35 <211> 2389 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(2359) <223> RXA00867 <400> 35 agagaactgg taaggttttt accgttctag accgcagaaa tcttcgcggc gacaccgatg 60 atcgccgagc agaactaaac atgaggagac ctactcgcat atg agc gat gta aag 115 Met Ser Asp Val Lys 1 5 tac ttc gaa gac acc gaa ttt ggc ctg atc gag gcc gtc gca acc atc 163 Tyr Phe Glu Asp Thr Glu Phe Gly Leu Ile Glu Ala Val Ala Thr Ile 10 15 20 gac aac ggt gac ttc gga acc cgc acc atc cgt ttt gaa acc ggc caa 211 Asp Asn Gly Asp Phe Gly Thr Arg Thr Ile Arg Phe Glu Thr Gly Gln 25 30 35 ctt gcc cgc cag gca gat ggt gca gtg acc acc tac ctc gac gat gac 259 Leu Ala Arg Gln Ala Asp Gly Ala Val Thr Thr Tyr Leu Asp Asp Asp 40 45 50 acg atg ctg ctg gca acc acc acc gca tcc aac cag cca cgc gag ggc 307 Thr Met Leu Leu Ala Thr Thr Thr Ala Ser Asn Gln Pro Arg Glu Gly 55 60 65 ttt gac ttc ttc cca ctg acc gtg gac gtt gaa gag cgt atg tac gca 355 Phe Asp Phe Phe Pro Leu Thr Val Asp Val Glu Glu Arg Met Tyr Ala 70 75 80 85 gct ggt cgc atc cct ggc tct ttc ttc cgt cgg gag ggt cgc cca tcc 403 Ala Gly Arg Ile Pro Gly Ser Phe Phe Arg Arg Glu Gly Arg Pro Ser 90 95 100 acc gaa gct atc ctg gct tgc cgt ctc atc gac cgc cca ctg cgc cca 451 Thr Glu Ala Ile Leu Ala Cys Arg Leu Ile Asp Arg Pro Leu Arg Pro 105 110 115 acc ttt gtt aag ggc ctg cgc aat gag gtt cag atc gtt gtc acc gtc 499 Thr Phe Val Lys Gly Leu Arg Asn Glu Val Gln Ile Val Val Thr Val 120 125 130 atg tcc atg aac cct gag gat tac tac gat gtc gta gca atc aac gga 547 Met Ser Met Asn Pro Glu Asp Tyr Tyr Asp Val Val Ala Ile Asn Gly 135 140 145 gct tcc gca gca acc cgc atc tcc gga ctt cct gtc tcc ggc gct gtc 595 Ala Ser Ala Ala Thr Arg Ile Ser Gly Leu Pro Val Ser Gly Ala Val 150 155 160 165 ggt ggc gtt cgc atg gca ctg gtt gtt gat gaa aag cac cca gaa ggc 643 Gly Gly Val Arg Met Ala Leu Val Val Asp Glu Lys His Pro Glu Gly 170 175 180 caa tgg gtt gca ttc cca acc cac gct caa cat gag cag tcc gta ttt 691 Gln Trp Val Ala Phe Pro Thr His Ala Gln His Glu Gln Ser Val Phe 185 190 195 gaa atc gtt gtg gct ggt cgc ctc gtc gag cgc aag cgc ggc aac aag 739 Glu Ile Val Val Ala Gly Arg Leu Val Glu Arg Lys Arg Gly Asn Lys 200 205 210 acc ttc tcc gac gtc gca gtg atg atg gtg gaa gct ggc gct tcc gaa 787 Thr Phe Ser Asp Val Ala Val Met Met Val Glu Ala Gly Ala Ser Glu 215 220 225 aac gtt gtc aac cgc gtc aag gac ggt gca cca gca cca acc gaa aag 835 Asn Val Val Asn Arg Val Lys Asp Gly Ala Pro Ala Pro Thr Glu Lys 230 235 240 245 atc gtc tcc gac ggc ctt gaa gca gct aag cca ttc atc gac atc ctg 883 Ile Val Ser Asp Gly Leu Glu Ala Ala Lys Pro Phe Ile Asp Ile Leu 250 255 260 tgc cgc gca cag gaa ggt ctg gca cag cgc gtt gga aac gca gcc aag 931 Cys Arg Ala Gln Glu Gly Leu Ala Gln Arg Val Gly Asn Ala Ala Lys 265 270 275 gaa ttc cca ctg ttc cct cca tac acc gac gag gtg tac tcc gca gtg 979 Glu Phe Pro Leu Phe Pro Pro Tyr Thr Asp Glu Val Tyr Ser Ala Val 280 285 290 gag cgc aag gta tcc aag aag cta gct tct ttg ctg acc ctg aag gca 1027 Glu Arg Lys Val Ser Lys Lys Leu Ala Ser Leu Leu Thr Leu Lys Ala 295 300 305 aag caa gag cgc gac gac gct acc aac gcc tac atg gaa gaa atc gaa 1075 Lys Gln Glu Arg Asp Asp Ala Thr Asn Ala Tyr Met Glu Glu Ile Glu 310 315 320 325 gcc gaa ctg ctt cca aag ttc gag gct tcc tac agc tca gca gct gaa 1123 Ala Glu Leu Leu Pro Lys Phe Glu Ala Ser Tyr Ser Ser Ala Ala Glu 330 335 340 gcg tcc aag gaa atc cgt gca gga tac aac gct gtc atg aag gcc atc 1171 Ala Ser Lys Glu Ile Arg Ala Gly Tyr Asn Ala Val Met Lys Ala Ile 345 350 355 gtg cgc cgc atg atc ctc acc gat cac ttc cgc atc gac ggc cgc gga 1219 Val Arg Arg Met Ile Leu Thr Asp His Phe Arg Ile Asp Gly Arg Gly 360 365 370 gtc acc gac atc cgt gac ctg gca gta gaa gtt gag ctc atc cca cgt 1267 Val Thr Asp Ile Arg Asp Leu Ala Val Glu Val Glu Leu Ile Pro Arg 375 380 385 gcg cac ggt tcc tcc ctc ttc gag cgt ggc gag acc cag atc ctt ggt 1315 Ala His Gly Ser Ser Leu Phe Glu Arg Gly Glu Thr Gln Ile Leu Gly 390 395 400 405 gtc acc acc ctg gac atg ctc aag atg gaa cag caa atc gac tcc ctg 1363 Val Thr Thr Leu Asp Met Leu Lys Met Glu Gln Gln Ile Asp Ser Leu 410 415 420 gca cca ggc gat gcg aag cgc tac atg cac cac tac aac ttc cct cca 1411 Ala Pro Gly Asp Ala Lys Arg Tyr Met His His Tyr Asn Phe Pro Pro 425 430 435 tac tcc acc ggt gaa acc ggt cgt gtg ggc tca cca aag cgc cgc gaa 1459 Tyr Ser Thr Gly Glu Thr Gly Arg Val Gly Ser Pro Lys Arg Arg Glu 440 445 450 atc ggc cac ggt gca ctt gca gaa cgc gca gtt ttg cca gta atc cca 1507 Ile Gly His Gly Ala Leu Ala Glu Arg Ala Val Leu Pro Val Ile Pro 455 460 465 tcc cgt gag gaa ttc cca tac gca atc cgt cag gtc tct gaa gct ctg 1555 Ser Arg Glu Glu Phe Pro Tyr Ala Ile Arg Gln Val Ser Glu Ala Leu 470 475 480 485 ggc tcc aac ggc tcc acc tcc atg ggc tct gtc tgt gca tcc act ctg 1603 Gly Ser Asn Gly Ser Thr Ser Met Gly Ser Val Cys Ala Ser Thr Leu 490 495 500 tcc ctg tac aac gct ggt gtt cca ctg aag gca cct gtt gca ggt atc 1651 Ser Leu Tyr Asn Ala Gly Val Pro Leu Lys Ala Pro Val Ala Gly Ile 505 510 515 gcc atg gga ctt gtt tcc ggt gaa atc gac ggc aag acc gag tac gtt 1699 Ala Met Gly Leu Val Ser Gly Glu Ile Asp Gly Lys Thr Glu Tyr Val 520 525 530 gca ctg acc gac atc ctc ggc gca gaa gac gca ttc ggc gac atg gac 1747 Ala Leu Thr Asp Ile Leu Gly Ala Glu Asp Ala Phe Gly Asp Met Asp 535 540 545 ttc aag gtt gcc ggc acc gca gac ttc atc acc gca ctt cag ctg gac 1795 Phe Lys Val Ala Gly Thr Ala Asp Phe Ile Thr Ala Leu Gln Leu Asp 550 555 560 565 acc aag ctg gac ggc att cct tcc aag gtg ctc tcc gat gcg ctt gag 1843 Thr Lys Leu Asp Gly Ile Pro Ser Lys Val Leu Ser Asp Ala Leu Glu 570 575 580 cag gca cgc gat gcc cga ctg acc atc ctg aac acc atg gct gat gtc 1891 Gln Ala Arg Asp Ala Arg Leu Thr Ile Leu Asn Thr Met Ala Asp Val 585 590 595 atc aac gga cct gat gag atg agc aag ttc gct cct cgc atc acc acc 1939 Ile Asn Gly Pro Asp Glu Met Ser Lys Phe Ala Pro Arg Ile Thr Thr 600 605 610 gtg aag atc cca gtg gca aag atc ggt gag ctg atc gga cca aag ggt 1987 Val Lys Ile Pro Val Ala Lys Ile Gly Glu Leu Ile Gly Pro Lys Gly 615 620 625 aag aac atc aac gct ctt acc gaa gag acc ggc gca aac atc tcc atc 2035 Lys Asn Ile Asn Ala Leu Thr Glu Glu Thr Gly Ala Asn Ile Ser Ile 630 635 640 645 gaa gat gac ggc acc gtg ttc atc tct gca gct gac ggc gca tct gct 2083 Glu Asp Asp Gly Thr Val Phe Ile Ser Ala Ala Asp Gly Ala Ser Ala 650 655 660 gaa gcg gcg atc gaa aag atc aac gct ctg gcg aac cca cag ctg cca 2131 Glu Ala Ala Ile Glu Lys Ile Asn Ala Leu Ala Asn Pro Gln Leu Pro 665 670 675 aag gtt ggc gag cgc ttc ctc gga acc gtc gtc aag acc acc gca ttc 2179 Lys Val Gly Glu Arg Phe Leu Gly Thr Val Val Lys Thr Thr Ala Phe 680 685 690 gga gca ttc gtt tcc ttg ctc cca ggc cgc gac ggc ctt gtt cac atc 2227 Gly Ala Phe Val Ser Leu Leu Pro Gly Arg Asp Gly Leu Val His Ile 695 700 705 tcc aag ctg ggt aac ggc aag cga gta gaa aag gtc gac gat gtg gtg 2275 Ser Lys Leu Gly Asn Gly Lys Arg Val Glu Lys Val Asp Asp Val Val 710 715 720 725 aag gtt ggc gag aag att cag gtc gaa atc gct gac atc gac aac cgc 2323 Lys Val Gly Glu Lys Ile Gln Val Glu Ile Ala Asp Ile Asp Asn Arg 730 735 740 ggc aag atc tcc ttg gtc cca gtt gtt gaa gag gac taattagttc 2369 Gly Lys Ile Ser Leu Val Pro Val Val Glu Glu Asp 745 750 tggctagatc gggctagatc 2389 <210> 36 <211> 753 <212> PRT <213> Corynebacterium glutamicum <400> 36 Met Ser Asp Val Lys Tyr Phe Glu Asp Thr Glu Phe Gly Leu Ile Glu 1 5 10 15 Ala Val Ala Thr Ile Asp Asn Gly Asp Phe Gly Thr Arg Thr Ile Arg 20 25 30 Phe Glu Thr Gly Gln Leu Ala Arg Gln Ala Asp Gly Ala Val Thr Thr 35 40 45 Tyr Leu Asp Asp Asp Thr Met Leu Leu Ala Thr Thr Thr Ala Ser Asn 50 55 60 Gln Pro Arg Glu Gly Phe Asp Phe Phe Pro Leu Thr Val Asp Val Glu 65 70 75 80 Glu Arg Met Tyr Ala Ala Gly Arg Ile Pro Gly Ser Phe Phe Arg Arg 85 90 95 Glu Gly Arg Pro Ser Thr Glu Ala Ile Leu Ala Cys Arg Leu Ile Asp 100 105 110 Arg Pro Leu Arg Pro Thr Phe Val Lys Gly Leu Arg Asn Glu Val Gln 115 120 125 Ile Val Val Thr Val Met Ser Met Asn Pro Glu Asp Tyr Tyr Asp Val 130 135 140 Val Ala Ile Asn Gly Ala Ser Ala Ala Thr Arg Ile Ser Gly Leu Pro 145 150 155 160 Val Ser Gly Ala Val Gly Gly Val Arg Met Ala Leu Val Val Asp Glu 165 170 175 Lys His Pro Glu Gly Gln Trp Val Ala Phe Pro Thr His Ala Gln His 180 185 190 Glu Gln Ser Val Phe Glu Ile Val Val Ala Gly Arg Leu Val Glu Arg 195 200 205 Lys Arg Gly Asn Lys Thr Phe Ser Asp Val Ala Val Met Met Val Glu 210 215 220 Ala Gly Ala Ser Glu Asn Val Val Asn Arg Val Lys Asp Gly Ala Pro 225 230 235 240 Ala Pro Thr Glu Lys Ile Val Ser Asp Gly Leu Glu Ala Ala Lys Pro 245 250 255 Phe Ile Asp Ile Leu Cys Arg Ala Gln Glu Gly Leu Ala Gln Arg Val 260 265 270 Gly Asn Ala Ala Lys Glu Phe Pro Leu Phe Pro Pro Tyr Thr Asp Glu 275 280 285 Val Tyr Ser Ala Val Glu Arg Lys Val Ser Lys Lys Leu Ala Ser Leu 290 295 300 Leu Thr Leu Lys Ala Lys Gln Glu Arg Asp Asp Ala Thr Asn Ala Tyr 305 310 315 320 Met Glu Glu Ile Glu Ala Glu Leu Leu Pro Lys Phe Glu Ala Ser Tyr 325 330 335 Ser Ser Ala Ala Glu Ala Ser Lys Glu Ile Arg Ala Gly Tyr Asn Ala 340 345 350 Val Met Lys Ala Ile Val Arg Arg Met Ile Leu Thr Asp His Phe Arg 355 360 365 Ile Asp Gly Arg Gly Val Thr Asp Ile Arg Asp Leu Ala Val Glu Val 370 375 380 Glu Leu Ile Pro Arg Ala His Gly Ser Ser Leu Phe Glu Arg Gly Glu 385 390 395 400 Thr Gln Ile Leu Gly Val Thr Thr Leu Asp Met Leu Lys Met Glu Gln 405 410 415 Gln Ile Asp Ser Leu Ala Pro Gly Asp Ala Lys Arg Tyr Met His His 420 425 430 Tyr Asn Phe Pro Pro Tyr Ser Thr Gly Glu Thr Gly Arg Val Gly Ser 435 440 445 Pro Lys Arg Arg Glu Ile Gly His Gly Ala Leu Ala Glu Arg Ala Val 450 455 460 Leu Pro Val Ile Pro Ser Arg Glu Glu Phe Pro Tyr Ala Ile Arg Gln 465 470 475 480 Val Ser Glu Ala Leu Gly Ser Asn Gly Ser Thr Ser Met Gly Ser Val 485 490 495 Cys Ala Ser Thr Leu Ser Leu Tyr Asn Ala Gly Val Pro Leu Lys Ala 500 505 510 Pro Val Ala Gly Ile Ala Met Gly Leu Val Ser Gly Glu Ile Asp Gly 515 520 525 Lys Thr Glu Tyr Val Ala Leu Thr Asp Ile Leu Gly Ala Glu Asp Ala 530 535 540 Phe Gly Asp Met Asp Phe Lys Val Ala Gly Thr Ala Asp Phe Ile Thr 545 550 555 560 Ala Leu Gln Leu Asp Thr Lys Leu Asp Gly Ile Pro Ser Lys Val Leu 565 570 575 Ser Asp Ala Leu Glu Gln Ala Arg Asp Ala Arg Leu Thr Ile Leu Asn 580 585 590 Thr Met Ala Asp Val Ile Asn Gly Pro Asp Glu Met Ser Lys Phe Ala 595 600 605 Pro Arg Ile Thr Thr Val Lys Ile Pro Val Ala Lys Ile Gly Glu Leu 610 615 620 Ile Gly Pro Lys Gly Lys Asn Ile Asn Ala Leu Thr Glu Glu Thr Gly 625 630 635 640 Ala Asn Ile Ser Ile Glu Asp Asp Gly Thr Val Phe Ile Ser Ala Ala 645 650 655 Asp Gly Ala Ser Ala Glu Ala Ala Ile Glu Lys Ile Asn Ala Leu Ala 660 665 670 Asn Pro Gln Leu Pro Lys Val Gly Glu Arg Phe Leu Gly Thr Val Val 675 680 685 Lys Thr Thr Ala Phe Gly Ala Phe Val Ser Leu Leu Pro Gly Arg Asp 690 695 700 Gly Leu Val His Ile Ser Lys Leu Gly Asn Gly Lys Arg Val Glu Lys 705 710 715 720 Val Asp Asp Val Val Lys Val Gly Glu Lys Ile Gln Val Glu Ile Ala 725 730 735 Asp Ile Asp Asn Arg Gly Lys Ile Ser Leu Val Pro Val Val Glu Glu 740 745 750 Asp <210> 37 <211> 1624 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1594) <223> RXA00955 <400> 37 gcacaccgtg atgcgttggc tgcgtccgca ggtgcgatgt tctacctcaa cggcgatgtc 60 gactccttga aagatggtgc acaaaaggcg ctttccttgc ttg ccg acg gca cca 115 Leu Pro Thr Ala Pro 1 5 ccc agg cat ggt tgg cca agc acg aag aga tcg att act cag aaa agg 163 Pro Arg His Gly Trp Pro Ser Thr Lys Arg Ser Ile Thr Gln Lys Arg 10 15 20 agt ctt cca atg act agt aat aat ctg ccc aca gtg ttg gaa agc atc 211 Ser Leu Pro Met Thr Ser Asn Asn Leu Pro Thr Val Leu Glu Ser Ile 25 30 35 gtc gag ggt cgt cgc gga cac ctg gag gaa att cgc gct cgc atc gct 259 Val Glu Gly Arg Arg Gly His Leu Glu Glu Ile Arg Ala Arg Ile Ala 40 45 50 cac gtg gat gtg gat gcg ctt cca aaa tcc acc cgt tct ctg ttt gat 307 His Val Asp Val Asp Ala Leu Pro Lys Ser Thr Arg Ser Leu Phe Asp 55 60 65 tcc ctc aac cag ggt agg gga ggg gcg cgt ttc atc atg gag tgc aag 355 Ser Leu Asn Gln Gly Arg Gly Gly Ala Arg Phe Ile Met Glu Cys Lys 70 75 80 85 tcc gca tcg cct tct ttg gga atg att cgt gag cac tac cag ccg ggt 403 Ser Ala Ser Pro Ser Leu Gly Met Ile Arg Glu His Tyr Gln Pro Gly 90 95 100 gaa atc gct cgc gtg tac tct cgc tac gcc agc ggc att tcc gtg ctg 451 Glu Ile Ala Arg Val Tyr Ser Arg Tyr Ala Ser Gly Ile Ser Val Leu 105 110 115 tgc gag ccg gat cgt ttt ggt ggc gat tac gat cac ctc gct acc gtt 499 Cys Glu Pro Asp Arg Phe Gly Gly Asp Tyr Asp His Leu Ala Thr Val 120 125 130 gcc gct acc tct cat ctt ccg gtg ctg tgc aaa gac ttc atc att gat 547 Ala Ala Thr Ser His Leu Pro Val Leu Cys Lys Asp Phe Ile Ile Asp 135 140 145 cct gtc cag gta cac gcg gcg cgt tac ttt ggt gct gat gcc atc ctg 595 Pro Val Gln Val His Ala Ala Arg Tyr Phe Gly Ala Asp Ala Ile Leu 150 155 160 165 ctc atg ctc tct gtg ctt gat gat gaa gag tac gca gca ctc gct gcc 643 Leu Met Leu Ser Val Leu Asp Asp Glu Glu Tyr Ala Ala Leu Ala Ala 170 175 180 gag gct gcg cgt ttt gat ctg gat atc ctc acc gag gtt att gat gag 691 Glu Ala Ala Arg Phe Asp Leu Asp Ile Leu Thr Glu Val Ile Asp Glu 185 190 195 gag gaa gtc gcc cgc gcc atc aag ctg ggt gcg aag atc ttt ggc gtc 739 Glu Glu Val Ala Arg Ala Ile Lys Leu Gly Ala Lys Ile Phe Gly Val 200 205 210 aac cac cgc aac ctg cat gat ctg tcc att gat ttg gat cgt tca cgt 787 Asn His Arg Asn Leu His Asp Leu Ser Ile Asp Leu Asp Arg Ser Arg 215 220 225 cgc ctg tcc aag ctc att cca gca gat gcc gtg ctc gtg tct gag tct 835 Arg Leu Ser Lys Leu Ile Pro Ala Asp Ala Val Leu Val Ser Glu Ser 230 235 240 245 ggc gtg cgc gat acc gaa acc gtc cgc cag cta ggt ggg cac tcc aat 883 Gly Val Arg Asp Thr Glu Thr Val Arg Gln Leu Gly Gly His Ser Asn 250 255 260 gca ttc ctc gtt ggc tcc cag ctg acc agc cag gaa aac gtc gat ctg 931 Ala Phe Leu Val Gly Ser Gln Leu Thr Ser Gln Glu Asn Val Asp Leu 265 270 275 gca gcc cgc gaa tta gtc tac ggc ccc aac aaa gtc tgc gga ctc acc 979 Ala Ala Arg Glu Leu Val Tyr Gly Pro Asn Lys Val Cys Gly Leu Thr 280 285 290 tca cca agt gca gca caa acc gct cgc gca gcg ggt gcg gtc tac ggc 1027 Ser Pro Ser Ala Ala Gln Thr Ala Arg Ala Ala Gly Ala Val Tyr Gly 295 300 305 ggg ctc atc ttc gaa gag gca tcg cca cgc aat gtt tca cgt gaa aca 1075 Gly Leu Ile Phe Glu Glu Ala Ser Pro Arg Asn Val Ser Arg Glu Thr 310 315 320 325 ttg caa aaa atc atc gcc gca gag ccc aac ctg cgc tac gtc gcg gtc 1123 Leu Gln Lys Ile Ile Ala Ala Glu Pro Asn Leu Arg Tyr Val Ala Val 330 335 340 agc cgt cgc acc tcc ggg tac aag gat ttg ctt gtc gac ggc atc ttc 1171 Ser Arg Arg Thr Ser Gly Tyr Lys Asp Leu Leu Val Asp Gly Ile Phe 345 350 355 gcc gta caa atc cac gcc cca ctg cag gac agc gtc gaa gca gaa aag 1219 Ala Val Gln Ile His Ala Pro Leu Gln Asp Ser Val Glu Ala Glu Lys 360 365 370 gca ttg atc gcc gcc gtt cgt gaa gag gtt gga ccg cag gtc cag gtc 1267 Ala Leu Ile Ala Ala Val Arg Glu Glu Val Gly Pro Gln Val Gln Val 375 380 385 tgg cgc gcg atc tcg atg tcc agc ccc ttg ggg gct gaa gtg gca gct 1315 Trp Arg Ala Ile Ser Met Ser Ser Pro Leu Gly Ala Glu Val Ala Ala 390 395 400 405 gcg gtg gag ggt gac gtc gat aag cta att ctt gat gcc cat gaa ggt 1363 Ala Val Glu Gly Asp Val Asp Lys Leu Ile Leu Asp Ala His Glu Gly 410 415 420 ggc agc ggg gaa gta ttc gac tgg gct acg gtg ccg gcc gct gtg aag 1411 Gly Ser Gly Glu Val Phe Asp Trp Ala Thr Val Pro Ala Ala Val Lys 425 430 435 gca aag tct ttg ctc gcg gga ggc atc tct ccg gac aac gct gcg cag 1459 Ala Lys Ser Leu Leu Ala Gly Gly Ile Ser Pro Asp Asn Ala Ala Gln 440 445 450 gca ctc gct gtg ggc tgc gca ggt ttg gac atc aac tct ggc gtg gaa 1507 Ala Leu Ala Val Gly Cys Ala Gly Leu Asp Ile Asn Ser Gly Val Glu 455 460 465 tac ccc gcc ggt gca ggc acg tgg gct ggg gcg aaa gac gcc ggc gcg 1555 Tyr Pro Ala Gly Ala Gly Thr Trp Ala Gly Ala Lys Asp Ala Gly Ala 470 475 480 485 ctg ctg aaa att tta gcg acc atc tcc aca ttc cat tac taaaggttta 1604 Leu Leu Lys Ile Leu Ala Thr Ile Ser Thr Phe His Tyr 490 495 aataggatca tgactgaaaa 1624 <210> 38 <211> 498 <212> PRT <213> Corynebacterium glutamicum <400> 38 Leu Pro Thr Ala Pro Pro Arg His Gly Trp Pro Ser Thr Lys Arg Ser 1 5 10 15 Ile Thr Gln Lys Arg Ser Leu Pro Met Thr Ser Asn Asn Leu Pro Thr 20 25 30 Val Leu Glu Ser Ile Val Glu Gly Arg Arg Gly His Leu Glu Glu Ile 35 40 45 Arg Ala Arg Ile Ala His Val Asp Val Asp Ala Leu Pro Lys Ser Thr 50 55 60 Arg Ser Leu Phe Asp Ser Leu Asn Gln Gly Arg Gly Gly Ala Arg Phe 65 70 75 80 Ile Met Glu Cys Lys Ser Ala Ser Pro Ser Leu Gly Met Ile Arg Glu 85 90 95 His Tyr Gln Pro Gly Glu Ile Ala Arg Val Tyr Ser Arg Tyr Ala Ser 100 105 110 Gly Ile Ser Val Leu Cys Glu Pro Asp Arg Phe Gly Gly Asp Tyr Asp 115 120 125 His Leu Ala Thr Val Ala Ala Thr Ser His Leu Pro Val Leu Cys Lys 130 135 140 Asp Phe Ile Ile Asp Pro Val Gln Val His Ala Ala Arg Tyr Phe Gly 145 150 155 160 Ala Asp Ala Ile Leu Leu Met Leu Ser Val Leu Asp Asp Glu Glu Tyr 165 170 175 Ala Ala Leu Ala Ala Glu Ala Ala Arg Phe Asp Leu Asp Ile Leu Thr 180 185 190 Glu Val Ile Asp Glu Glu Glu Val Ala Arg Ala Ile Lys Leu Gly Ala 195 200 205 Lys Ile Phe Gly Val Asn His Arg Asn Leu His Asp Leu Ser Ile Asp 210 215 220 Leu Asp Arg Ser Arg Arg Leu Ser Lys Leu Ile Pro Ala Asp Ala Val 225 230 235 240 Leu Val Ser Glu Ser Gly Val Arg Asp Thr Glu Thr Val Arg Gln Leu 245 250 255 Gly Gly His Ser Asn Ala Phe Leu Val Gly Ser Gln Leu Thr Ser Gln 260 265 270 Glu Asn Val Asp Leu Ala Ala Arg Glu Leu Val Tyr Gly Pro Asn Lys 275 280 285 Val Cys Gly Leu Thr Ser Pro Ser Ala Ala Gln Thr Ala Arg Ala Ala 290 295 300 Gly Ala Val Tyr Gly Gly Leu Ile Phe Glu Glu Ala Ser Pro Arg Asn 305 310 315 320 Val Ser Arg Glu Thr Leu Gln Lys Ile Ile Ala Ala Glu Pro Asn Leu 325 330 335 Arg Tyr Val Ala Val Ser Arg Arg Thr Ser Gly Tyr Lys Asp Leu Leu 340 345 350 Val Asp Gly Ile Phe Ala Val Gln Ile His Ala Pro Leu Gln Asp Ser 355 360 365 Val Glu Ala Glu Lys Ala Leu Ile Ala Ala Val Arg Glu Glu Val Gly 370 375 380 Pro Gln Val Gln Val Trp Arg Ala Ile Ser Met Ser Ser Pro Leu Gly 385 390 395 400 Ala Glu Val Ala Ala Ala Val Glu Gly Asp Val Asp Lys Leu Ile Leu 405 410 415 Asp Ala His Glu Gly Gly Ser Gly Glu Val Phe Asp Trp Ala Thr Val 420 425 430 Pro Ala Ala Val Lys Ala Lys Ser Leu Leu Ala Gly Gly Ile Ser Pro 435 440 445 Asp Asn Ala Ala Gln Ala Leu Ala Val Gly Cys Ala Gly Leu Asp Ile 450 455 460 Asn Ser Gly Val Glu Tyr Pro Ala Gly Ala Gly Thr Trp Ala Gly Ala 465 470 475 480 Lys Asp Ala Gly Ala Leu Leu Lys Ile Leu Ala Thr Ile Ser Thr Phe 485 490 495 His Tyr <210> 39 <211> 1684 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1654) <223> RXA00957 <400> 39 aaccggcaag ccctggatcg aatgaagctc gcagcgagta attatttgat gtttcccaga 60 aaggcttcag ccccacaatg atttcctcgg taggtgcccc atg agc acg aat ccc 115 Met Ser Thr Asn Pro 1 5 cat gtt ttc tcc cta gat gtc cgc tat cac gag gat gct tct gca ttg 163 His Val Phe Ser Leu Asp Val Arg Tyr His Glu Asp Ala Ser Ala Leu 10 15 20 ttt gcc cac ttg ggt ggc aca acc gca gat gat gca gcc ctg ttg gaa 211 Phe Ala His Leu Gly Gly Thr Thr Ala Asp Asp Ala Ala Leu Leu Glu 25 30 35 agc gct gat atc acc acc aag aat ggt att tct tcc ctc gcg gtg ttg 259 Ser Ala Asp Ile Thr Thr Lys Asn Gly Ile Ser Ser Leu Ala Val Leu 40 45 50 aag agt tcg gtg cgc att acg tgc acg ggc aac acg gtg gta acg cag 307 Lys Ser Ser Val Arg Ile Thr Cys Thr Gly Asn Thr Val Val Thr Gln 55 60 65 ccg ctg acg gac tcg ggt agg gca gtg gtt gcg cgc cta acg cag cag 355 Pro Leu Thr Asp Ser Gly Arg Ala Val Val Ala Arg Leu Thr Gln Gln 70 75 80 85 ctt ggc cag tac aac acc gca gag aac acc ttt agc ttc ccc gcc tca 403 Leu Gly Gln Tyr Asn Thr Ala Glu Asn Thr Phe Ser Phe Pro Ala Ser 90 95 100 gat gcg gtt gat gag cgc gag cgc ctc acc gca cca agc acc atc gaa 451 Asp Ala Val Asp Glu Arg Glu Arg Leu Thr Ala Pro Ser Thr Ile Glu 105 110 115 gtg ctg cgc aag ttg cag ttc gag tcc ggc tac agc gac gcg tcc ctg 499 Val Leu Arg Lys Leu Gln Phe Glu Ser Gly Tyr Ser Asp Ala Ser Leu 120 125 130 cca ctg ctc atg ggc ggt ttc gcg ttt gat ttc tta gaa acc ttt gaa 547 Pro Leu Leu Met Gly Gly Phe Ala Phe Asp Phe Leu Glu Thr Phe Glu 135 140 145 acg ctc ccc gct gtc gag gag agc gtc aac act tac ccc gat tac cag 595 Thr Leu Pro Ala Val Glu Glu Ser Val Asn Thr Tyr Pro Asp Tyr Gln 150 155 160 165 ttc gtc ctc gcg gaa atc gtc ctg gac atc aat cac cag gac cag acc 643 Phe Val Leu Ala Glu Ile Val Leu Asp Ile Asn His Gln Asp Gln Thr 170 175 180 gcc aaa ctc gcc ggc gtc tcc aac gcc cca ggc gag ctc gag gcc gag 691 Ala Lys Leu Ala Gly Val Ser Asn Ala Pro Gly Glu Leu Glu Ala Glu 185 190 195 ctc aac aag ctt tca ttg ctt atc gac gcc gcc ctc ccc gca acc gaa 739 Leu Asn Lys Leu Ser Leu Leu Ile Asp Ala Ala Leu Pro Ala Thr Glu 200 205 210 cac gcc tac caa acc acc cct cac gac ggc gac act ctt cgc gtt gtg 787 His Ala Tyr Gln Thr Thr Pro His Asp Gly Asp Thr Leu Arg Val Val 215 220 225 gct gat att ccc gat gct cag ttc cgc acc cag atc aat gag ctg aaa 835 Ala Asp Ile Pro Asp Ala Gln Phe Arg Thr Gln Ile Asn Glu Leu Lys 230 235 240 245 gaa aac att tac aac ggt gac atc tac caa gtt gtc ccg gcg cgc act 883 Glu Asn Ile Tyr Asn Gly Asp Ile Tyr Gln Val Val Pro Ala Arg Thr 250 255 260 ttc acc gca cca tgt cct gat gca ttc gct gct tat ctg cag ctg cgt 931 Phe Thr Ala Pro Cys Pro Asp Ala Phe Ala Ala Tyr Leu Gln Leu Arg 265 270 275 gcc acc aac ccg tcg ccg tac atg ttc tat atc cgt ggc ctc aac gaa 979 Ala Thr Asn Pro Ser Pro Tyr Met Phe Tyr Ile Arg Gly Leu Asn Glu 280 285 290 ggc cgc tcc tat gaa ctt ttt ggc gca tcc cct gag tcc aac ctc aag 1027 Gly Arg Ser Tyr Glu Leu Phe Gly Ala Ser Pro Glu Ser Asn Leu Lys 295 300 305 ttc acc gct gct aac cgt gag ctg cag ctg tac cca atc gca ggt acc 1075 Phe Thr Ala Ala Asn Arg Glu Leu Gln Leu Tyr Pro Ile Ala Gly Thr 310 315 320 325 cgc ccc cgt gga ctc aac cca gat ggc tcc atc aac gat gag cta gat 1123 Arg Pro Arg Gly Leu Asn Pro Asp Gly Ser Ile Asn Asp Glu Leu Asp 330 335 340 atc cgc aat gag ttg gat atg cgc act gat gcc aaa gag atc gcg gag 1171 Ile Arg Asn Glu Leu Asp Met Arg Thr Asp Ala Lys Glu Ile Ala Glu 345 350 355 cac acc atg ctt gtc gat ctc gcc cgc aac gac ctg gcc cgc gtc tcg 1219 His Thr Met Leu Val Asp Leu Ala Arg Asn Asp Leu Ala Arg Val Ser 360 365 370 gtc cca gcg tcg cgc cgg gtt gcg gat ctt ttg cag gtg gat cgc tat 1267 Val Pro Ala Ser Arg Arg Val Ala Asp Leu Leu Gln Val Asp Arg Tyr 375 380 385 tcc cgc gtg atg cac ttg gtg tcc cgt gtg acg gcg acg ttg gac cca 1315 Ser Arg Val Met His Leu Val Ser Arg Val Thr Ala Thr Leu Asp Pro 390 395 400 405 gag ctt gat gct ttg gac gcc tat cgg gcg tgc atg aat atg ggc acg 1363 Glu Leu Asp Ala Leu Asp Ala Tyr Arg Ala Cys Met Asn Met Gly Thr 410 415 420 ttg acc ggc gct ccg aag ttg cgc gct atg gag ctg ttg cgc ggc gtc 1411 Leu Thr Gly Ala Pro Lys Leu Arg Ala Met Glu Leu Leu Arg Gly Val 425 430 435 gaa aag cgc agg cgt ggt tct tat ggt ggg gca gtg ggg tac ctg cgc 1459 Glu Lys Arg Arg Arg Gly Ser Tyr Gly Gly Ala Val Gly Tyr Leu Arg 440 445 450 ggc aat ggc gat atg gat aat tgc att gtt att cgt tcg gcg ttt gtc 1507 Gly Asn Gly Asp Met Asp Asn Cys Ile Val Ile Arg Ser Ala Phe Val 455 460 465 cag gat ggt gtg gct gct gtg cag gct ggt gct ggt gtg gtc cgc gat 1555 Gln Asp Gly Val Ala Ala Val Gln Ala Gly Ala Gly Val Val Arg Asp 470 475 480 485 tct aat cct caa tct gaa gcc gat gag acg ttg cac aag gcg tat gcc 1603 Ser Asn Pro Gln Ser Glu Ala Asp Glu Thr Leu His Lys Ala Tyr Ala 490 495 500 gtg ttg aat gcc att gcg ctt gct gct ggt tcc act ttg gag gtc atc 1651 Val Leu Asn Ala Ile Ala Leu Ala Ala Gly Ser Thr Leu Glu Val Ile 505 510 515 cga tgacacacgt tgttctcatt gataatcacg 1684 Arg <210> 40 <211> 518 <212> PRT <213> Corynebacterium glutamicum <400> 40 Met Ser Thr Asn Pro His Val Phe Ser Leu Asp Val Arg Tyr His Glu 1 5 10 15 Asp Ala Ser Ala Leu Phe Ala His Leu Gly Gly Thr Thr Ala Asp Asp 20 25 30 Ala Ala Leu Leu Glu Ser Ala Asp Ile Thr Thr Lys Asn Gly Ile Ser 35 40 45 Ser Leu Ala Val Leu Lys Ser Ser Val Arg Ile Thr Cys Thr Gly Asn 50 55 60 Thr Val Val Thr Gln Pro Leu Thr Asp Ser Gly Arg Ala Val Val Ala 65 70 75 80 Arg Leu Thr Gln Gln Leu Gly Gln Tyr Asn Thr Ala Glu Asn Thr Phe 85 90 95 Ser Phe Pro Ala Ser Asp Ala Val Asp Glu Arg Glu Arg Leu Thr Ala 100 105 110 Pro Ser Thr Ile Glu Val Leu Arg Lys Leu Gln Phe Glu Ser Gly Tyr 115 120 125 Ser Asp Ala Ser Leu Pro Leu Leu Met Gly Gly Phe Ala Phe Asp Phe 130 135 140 Leu Glu Thr Phe Glu Thr Leu Pro Ala Val Glu Glu Ser Val Asn Thr 145 150 155 160 Tyr Pro Asp Tyr Gln Phe Val Leu Ala Glu Ile Val Leu Asp Ile Asn 165 170 175 His Gln Asp Gln Thr Ala Lys Leu Ala Gly Val Ser Asn Ala Pro Gly 180 185 190 Glu Leu Glu Ala Glu Leu Asn Lys Leu Ser Leu Leu Ile Asp Ala Ala 195 200 205 Leu Pro Ala Thr Glu His Ala Tyr Gln Thr Thr Pro His Asp Gly Asp 210 215 220 Thr Leu Arg Val Val Ala Asp Ile Pro Asp Ala Gln Phe Arg Thr Gln 225 230 235 240 Ile Asn Glu Leu Lys Glu Asn Ile Tyr Asn Gly Asp Ile Tyr Gln Val 245 250 255 Val Pro Ala Arg Thr Phe Thr Ala Pro Cys Pro Asp Ala Phe Ala Ala 260 265 270 Tyr Leu Gln Leu Arg Ala Thr Asn Pro Ser Pro Tyr Met Phe Tyr Ile 275 280 285 Arg Gly Leu Asn Glu Gly Arg Ser Tyr Glu Leu Phe Gly Ala Ser Pro 290 295 300 Glu Ser Asn Leu Lys Phe Thr Ala Ala Asn Arg Glu Leu Gln Leu Tyr 305 310 315 320 Pro Ile Ala Gly Thr Arg Pro Arg Gly Leu Asn Pro Asp Gly Ser Ile 325 330 335 Asn Asp Glu Leu Asp Ile Arg Asn Glu Leu Asp Met Arg Thr Asp Ala 340 345 350 Lys Glu Ile Ala Glu His Thr Met Leu Val Asp Leu Ala Arg Asn Asp 355 360 365 Leu Ala Arg Val Ser Val Pro Ala Ser Arg Arg Val Ala Asp Leu Leu 370 375 380 Gln Val Asp Arg Tyr Ser Arg Val Met His Leu Val Ser Arg Val Thr 385 390 395 400 Ala Thr Leu Asp Pro Glu Leu Asp Ala Leu Asp Ala Tyr Arg Ala Cys 405 410 415 Met Asn Met Gly Thr Leu Thr Gly Ala Pro Lys Leu Arg Ala Met Glu 420 425 430 Leu Leu Arg Gly Val Glu Lys Arg Arg Arg Gly Ser Tyr Gly Gly Ala 435 440 445 Val Gly Tyr Leu Arg Gly Asn Gly Asp Met Asp Asn Cys Ile Val Ile 450 455 460 Arg Ser Ala Phe Val Gln Asp Gly Val Ala Ala Val Gln Ala Gly Ala 465 470 475 480 Gly Val Val Arg Asp Ser Asn Pro Gln Ser Glu Ala Asp Glu Thr Leu 485 490 495 His Lys Ala Tyr Ala Val Leu Asn Ala Ile Ala Leu Ala Ala Gly Ser 500 505 510 Thr Leu Glu Val Ile Arg 515 <210> 41 <211> 1057 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1027) <223> RXA00970 <400> 41 gaatctgatc tttcccgcac cgttgaactg ctgaaggcta agcctgttgt taaggcaatc 60 aacagtgtga tccgcctcga aagggactaa ttttactgac atg gca att gaa ctg 115 Met Ala Ile Glu Leu 1 5 aac gtc ggt cgt aag gtt acc gtc acg gta cct gga tct tct gca aac 163 Asn Val Gly Arg Lys Val Thr Val Thr Val Pro Gly Ser Ser Ala Asn 10 15 20 ctc gga cct ggc ttt gac act tta ggt ttg gca ctg tcg gta tac gac 211 Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala Leu Ser Val Tyr Asp 25 30 35 act gtc gaa gtg gaa att att cca tct ggc ttg gaa gtg gaa gtt ttt 259 Thr Val Glu Val Glu Ile Ile Pro Ser Gly Leu Glu Val Glu Val Phe 40 45 50 ggc gaa ggc caa ggc gaa gtc cct ctt gat ggc tcc cac ctg gtg gtt 307 Gly Glu Gly Gln Gly Glu Val Pro Leu Asp Gly Ser His Leu Val Val 55 60 65 aaa gct att cgt gct ggc ctg aag gca gct gac gct gaa gtt cct gga 355 Lys Ala Ile Arg Ala Gly Leu Lys Ala Ala Asp Ala Glu Val Pro Gly 70 75 80 85 ttg cga gtg gtg tgc cac aac aac att ccg cag tct cgt ggt ctt ggc 403 Leu Arg Val Val Cys His Asn Asn Ile Pro Gln Ser Arg Gly Leu Gly 90 95 100 tcc tct gct gca gcg gcg gtt gct ggt gtt gct gca gct aat ggt ttg 451 Ser Ser Ala Ala Ala Ala Val Ala Gly Val Ala Ala Ala Asn Gly Leu 105 110 115 gcg gat ttc ccg ctg act caa gag cag att gtt cag ttg tcc tct gcc 499 Ala Asp Phe Pro Leu Thr Gln Glu Gln Ile Val Gln Leu Ser Ser Ala 120 125 130 ttt gaa ggc cac cca gat aat gct gcg gct tct gtg ctg ggt gga gca 547 Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser Val Leu Gly Gly Ala 135 140 145 gtg gtg tcg tgg aca aat ctg tct atc gac ggc aag agc cag cca cag 595 Val Val Ser Trp Thr Asn Leu Ser Ile Asp Gly Lys Ser Gln Pro Gln 150 155 160 165 tat gct gct gta cca ctt gag gtg cag gac aat att cgt gcg act gcg 643 Tyr Ala Ala Val Pro Leu Glu Val Gln Asp Asn Ile Arg Ala Thr Ala 170 175 180 ctg gtt cct aat ttc cac gca tcc acc gaa gct gtg cgc cga gtc ctt 691 Leu Val Pro Asn Phe His Ala Ser Thr Glu Ala Val Arg Arg Val Leu 185 190 195 ccc act gaa gtc act cac atc gat gcg cga ttt aac gtg tcc cgc gtt 739 Pro Thr Glu Val Thr His Ile Asp Ala Arg Phe Asn Val Ser Arg Val 200 205 210 gca gtg atg atc gtt gcg ttg cag cag cgt cct gat ttg ctg tgg gag 787 Ala Val Met Ile Val Ala Leu Gln Gln Arg Pro Asp Leu Leu Trp Glu 215 220 225 ggt act cgt gac cgt ctg cac cag cct tat cgt gca gaa gtg ttg cct 835 Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg Ala Glu Val Leu Pro 230 235 240 245 att acc tct gag tgg gta aac cgc ctg cgc aac cgt ggc tac gcg gca 883 Ile Thr Ser Glu Trp Val Asn Arg Leu Arg Asn Arg Gly Tyr Ala Ala 250 255 260 tac ctt tcc ggt gcc ggc cca acc gcc atg gtg ctg tcc act gag cca 931 Tyr Leu Ser Gly Ala Gly Pro Thr Ala Met Val Leu Ser Thr Glu Pro 265 270 275 att cca gac aag gtt ttg gaa gat gct cgt gag tct ggc att aag gtg 979 Ile Pro Asp Lys Val Leu Glu Asp Ala Arg Glu Ser Gly Ile Lys Val 280 285 290 ctt gag ctt gag gtt gcg gga cca gtc aag gtt gaa gtt aac caa cct 1027 Leu Glu Leu Glu Val Ala Gly Pro Val Lys Val Glu Val Asn Gln Pro 295 300 305 taggcccaac aaggaaggcc cccttcgaat 1057 <210> 42 <211> 309 <212> PRT <213> Corynebacterium glutamicum <400> 42 Met Ala Ile Glu Leu Asn Val Gly Arg Lys Val Thr Val Thr Val Pro 1 5 10 15 Gly Ser Ser Ala Asn Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala 20 25 30 Leu Ser Val Tyr Asp Thr Val Glu Val Glu Ile Ile Pro Ser Gly Leu 35 40 45 Glu Val Glu Val Phe Gly Glu Gly Gln Gly Glu Val Pro Leu Asp Gly 50 55 60 Ser His Leu Val Val Lys Ala Ile Arg Ala Gly Leu Lys Ala Ala Asp 65 70 75 80 Ala Glu Val Pro Gly Leu Arg Val Val Cys His Asn Asn Ile Pro Gln 85 90 95 Ser Arg Gly Leu Gly Ser Ser Ala Ala Ala Ala Val Ala Gly Val Ala 100 105 110 Ala Ala Asn Gly Leu Ala Asp Phe Pro Leu Thr Gln Glu Gln Ile Val 115 120 125 Gln Leu Ser Ser Ala Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser 130 135 140 Val Leu Gly Gly Ala Val Val Ser Trp Thr Asn Leu Ser Ile Asp Gly 145 150 155 160 Lys Ser Gln Pro Gln Tyr Ala Ala Val Pro Leu Glu Val Gln Asp Asn 165 170 175 Ile Arg Ala Thr Ala Leu Val Pro Asn Phe His Ala Ser Thr Glu Ala 180 185 190 Val Arg Arg Val Leu Pro Thr Glu Val Thr His Ile Asp Ala Arg Phe 195 200 205 Asn Val Ser Arg Val Ala Val Met Ile Val Ala Leu Gln Gln Arg Pro 210 215 220 Asp Leu Leu Trp Glu Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg 225 230 235 240 Ala Glu Val Leu Pro Ile Thr Ser Glu Trp Val Asn Arg Leu Arg Asn 245 250 255 Arg Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Ala Met Val 260 265 270 Leu Ser Thr Glu Pro Ile Pro Asp Lys Val Leu Glu Asp Ala Arg Glu 275 280 285 Ser Gly Ile Lys Val Leu Glu Leu Glu Val Ala Gly Pro Val Lys Val 290 295 300 Glu Val Asn Gln Pro 305 <210> 43 <211> 793 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(763) <223> RXA00997 <400> 43 acaacaacac cgacttcatt tctgctgtga gctccggtgt catcgtggcc gaagcaacgc 60 cgattcagct aggtggccgc acccatctgt ggcagatcga atg cac cca ccg cgg 115 Met His Pro Pro Arg 1 5 aga agt cgt cgc acg aac cac act gcg cac cat ggt gct gaa caa gta 163 Arg Ser Arg Arg Thr Asn His Thr Ala His His Gly Ala Glu Gln Val 10 15 20 gcc cta tac tcg ggc acc atg act aca tgg aaa gag ctc aca gat aac 211 Ala Leu Tyr Ser Gly Thr Met Thr Thr Trp Lys Glu Leu Thr Asp Asn 25 30 35 aac cca gcg cac tca gaa aac tac gcg cag cgc tgg cga aac ctc gcc 259 Asn Pro Ala His Ser Glu Asn Tyr Ala Gln Arg Trp Arg Asn Leu Ala 40 45 50 gca gca ggc aat gat att tac ggc gaa gcc cgc ctc att gat gcc atg 307 Ala Ala Gly Asn Asp Ile Tyr Gly Glu Ala Arg Leu Ile Asp Ala Met 55 60 65 gca ccc agg gga gcg aaa atc ttg gat gct ggc tgc ggc cag gga cgc 355 Ala Pro Arg Gly Ala Lys Ile Leu Asp Ala Gly Cys Gly Gln Gly Arg 70 75 80 85 atc ggt ggc tac ctg tcc aag caa ggc cac gat gtt cta ggc aca gac 403 Ile Gly Gly Tyr Leu Ser Lys Gln Gly His Asp Val Leu Gly Thr Asp 90 95 100 ctt gat ccc atc ctg att gat tac gcc aag cag gac ttt cca gaa gct 451 Leu Asp Pro Ile Leu Ile Asp Tyr Ala Lys Gln Asp Phe Pro Glu Ala 105 110 115 cgc tgg gtg gtg gga gat ctc tct gtt gat cag atc tca gag act gat 499 Arg Trp Val Val Gly Asp Leu Ser Val Asp Gln Ile Ser Glu Thr Asp 120 125 130 ttt gat ctc att gtc tcc gcc ggc aac gtc atg ggc ttt ctc gct gag 547 Phe Asp Leu Ile Val Ser Ala Gly Asn Val Met Gly Phe Leu Ala Glu 135 140 145 gat ggt cgc gaa cct gca cta gcc aac att cac cgc gca ctg ggc gcc 595 Asp Gly Arg Glu Pro Ala Leu Ala Asn Ile His Arg Ala Leu Gly Ala 150 155 160 165 gat ggc cgc gct gtc atc ggt ttc ggc gca gga cgt gga tgg gtc ttt 643 Asp Gly Arg Ala Val Ile Gly Phe Gly Ala Gly Arg Gly Trp Val Phe 170 175 180 gga gac ttc ctc gaa gtc gca gaa cgc gtg ggc ctc gag ttg gaa aat 691 Gly Asp Phe Leu Glu Val Ala Glu Arg Val Gly Leu Glu Leu Glu Asn 185 190 195 gct ttt gag tct tgg gat ctt aag cct ttt gtc caa ggc tct gag ttc 739 Ala Phe Glu Ser Trp Asp Leu Lys Pro Phe Val Gln Gly Ser Glu Phe 200 205 210 tta gtg gcg gta ttt acc aag aag taacacctct atcttgcacc tgatctggcg 793 Leu Val Ala Val Phe Thr Lys Lys 215 220 <210> 44 <211> 221 <212> PRT <213> Corynebacterium glutamicum <400> 44 Met His Pro Pro Arg Arg Ser Arg Arg Thr Asn His Thr Ala His His 1 5 10 15 Gly Ala Glu Gln Val Ala Leu Tyr Ser Gly Thr Met Thr Thr Trp Lys 20 25 30 Glu Leu Thr Asp Asn Asn Pro Ala His Ser Glu Asn Tyr Ala Gln Arg 35 40 45 Trp Arg Asn Leu Ala Ala Ala Gly Asn Asp Ile Tyr Gly Glu Ala Arg 50 55 60 Leu Ile Asp Ala Met Ala Pro Arg Gly Ala Lys Ile Leu Asp Ala Gly 65 70 75 80 Cys Gly Gln Gly Arg Ile Gly Gly Tyr Leu Ser Lys Gln Gly His Asp 85 90 95 Val Leu Gly Thr Asp Leu Asp Pro Ile Leu Ile Asp Tyr Ala Lys Gln 100 105 110 Asp Phe Pro Glu Ala Arg Trp Val Val Gly Asp Leu Ser Val Asp Gln 115 120 125 Ile Ser Glu Thr Asp Phe Asp Leu Ile Val Ser Ala Gly Asn Val Met 130 135 140 Gly Phe Leu Ala Glu Asp Gly Arg Glu Pro Ala Leu Ala Asn Ile His 145 150 155 160 Arg Ala Leu Gly Ala Asp Gly Arg Ala Val Ile Gly Phe Gly Ala Gly 165 170 175 Arg Gly Trp Val Phe Gly Asp Phe Leu Glu Val Ala Glu Arg Val Gly 180 185 190 Leu Glu Leu Glu Asn Ala Phe Glu Ser Trp Asp Leu Lys Pro Phe Val 195 200 205 Gln Gly Ser Glu Phe Leu Val Ala Val Phe Thr Lys Lys 210 215 220 <210> 45 <211> 931 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(901) <223> RXA01095 <400> 45 aagactcaga aatccaaaga aaccccaggt caaagctagg gtgtggcacc ctgatttctt 60 tcgccatgtg tgttcgggat aaccttaaac acagcattgg ttg gaa gga ggt tgg 115 Leu Glu Gly Gly Trp 1 5 ggc atg gtt gca aca gag aac cgc atg ttg atg gaa atc gct gcg gaa 163 Gly Met Val Ala Thr Glu Asn Arg Met Leu Met Glu Ile Ala Ala Glu 10 15 20 ata tcg gct cgg gaa gca acg ctt ggt ttt caa gaa gtc aaa act aaa 211 Ile Ser Ala Arg Glu Ala Thr Leu Gly Phe Gln Glu Val Lys Thr Lys 25 30 35 tct cga tca gca ggt ctc acg gcg gct ttc gat att gct tca gtc ttt 259 Ser Arg Ser Ala Gly Leu Thr Ala Ala Phe Asp Ile Ala Ser Val Phe 40 45 50 ttt tcg tct gga tgt aat gtc gta gtc gcc ttt gat cgt ttt gca tcc 307 Phe Ser Ser Gly Cys Asn Val Val Val Ala Phe Asp Arg Phe Ala Ser 55 60 65 aat tgg tct gat cat tcg gat cat gtg gac tac gct gca cag gtt gcg 355 Asn Trp Ser Asp His Ser Asp His Val Asp Tyr Ala Ala Gln Val Ala 70 75 80 85 ggt ttt ggc gca tca atg ctt gca tat acg gtg cgc agg gga cag ttt 403 Gly Phe Gly Ala Ser Met Leu Ala Tyr Thr Val Arg Arg Gly Gln Phe 90 95 100 gat acc gca gta cgc gat atc agg gac atc aaa tct gaa gta gac att 451 Asp Thr Ala Val Arg Asp Ile Arg Asp Ile Lys Ser Glu Val Asp Ile 105 110 115 ccc att ctg ctt cat gat ccc atc atc gat ccg tat caa atc cac gaa 499 Pro Ile Leu Leu His Asp Pro Ile Ile Asp Pro Tyr Gln Ile His Glu 120 125 130 gcc cgc gtc atg ggc atc gac gct ctt caa ttc ccc gta tgg gcg atg 547 Ala Arg Val Met Gly Ile Asp Ala Leu Gln Phe Pro Val Trp Ala Met 135 140 145 gaa caa gct cga ctg gaa tct ttg gtg gac cgc acc gaa tca ttg ggc 595 Glu Gln Ala Arg Leu Glu Ser Leu Val Asp Arg Thr Glu Ser Leu Gly 150 155 160 165 atg aca gcc atc gtg tct gtg cga aac cac gaa gaa gcg cat cgt gca 643 Met Thr Ala Ile Val Ser Val Arg Asn His Glu Glu Ala His Arg Ala 170 175 180 gtg gac gca gga gcg aca gtg gta gca att gat att act ggt tat acc 691 Val Asp Ala Gly Ala Thr Val Val Ala Ile Asp Ile Thr Gly Tyr Thr 185 190 195 ggc tca ctc act ttg cct gaa gcg ttt tcg ggt atc acc caa ttc atg 739 Gly Ser Leu Thr Leu Pro Glu Ala Phe Ser Gly Ile Thr Gln Phe Met 200 205 210 ccc aaa gag gta gcc cgc att gtg ctc gga ggt tgc agc agc cct aaa 787 Pro Lys Glu Val Ala Arg Ile Val Leu Gly Gly Cys Ser Ser Pro Lys 215 220 225 gaa ctc atg cgg ttt gca cga cat tct gca gac gcc atc ttt gtt cca 835 Glu Leu Met Arg Phe Ala Arg His Ser Ala Asp Ala Ile Phe Val Pro 230 235 240 245 cat gca gac ctc gcc acc aca aaa tct ctt gtg aca gca ggt atg cat 883 His Ala Asp Leu Ala Thr Thr Lys Ser Leu Val Thr Ala Gly Met His 250 255 260 cca gcg tgc cca tcg cgt tgaagaggtg ctctgtggtc agctgaccgg 931 Pro Ala Cys Pro Ser Arg 265 <210> 46 <211> 267 <212> PRT <213> Corynebacterium glutamicum <400> 46 Leu Glu Gly Gly Trp Gly Met Val Ala Thr Glu Asn Arg Met Leu Met 1 5 10 15 Glu Ile Ala Ala Glu Ile Ser Ala Arg Glu Ala Thr Leu Gly Phe Gln 20 25 30 Glu Val Lys Thr Lys Ser Arg Ser Ala Gly Leu Thr Ala Ala Phe Asp 35 40 45 Ile Ala Ser Val Phe Phe Ser Ser Gly Cys Asn Val Val Val Ala Phe 50 55 60 Asp Arg Phe Ala Ser Asn Trp Ser Asp His Ser Asp His Val Asp Tyr 65 70 75 80 Ala Ala Gln Val Ala Gly Phe Gly Ala Ser Met Leu Ala Tyr Thr Val 85 90 95 Arg Arg Gly Gln Phe Asp Thr Ala Val Arg Asp Ile Arg Asp Ile Lys 100 105 110 Ser Glu Val Asp Ile Pro Ile Leu Leu His Asp Pro Ile Ile Asp Pro 115 120 125 Tyr Gln Ile His Glu Ala Arg Val Met Gly Ile Asp Ala Leu Gln Phe 130 135 140 Pro Val Trp Ala Met Glu Gln Ala Arg Leu Glu Ser Leu Val Asp Arg 145 150 155 160 Thr Glu Ser Leu Gly Met Thr Ala Ile Val Ser Val Arg Asn His Glu 165 170 175 Glu Ala His Arg Ala Val Asp Ala Gly Ala Thr Val Val Ala Ile Asp 180 185 190 Ile Thr Gly Tyr Thr Gly Ser Leu Thr Leu Pro Glu Ala Phe Ser Gly 195 200 205 Ile Thr Gln Phe Met Pro Lys Glu Val Ala Arg Ile Val Leu Gly Gly 210 215 220 Cys Ser Ser Pro Lys Glu Leu Met Arg Phe Ala Arg His Ser Ala Asp 225 230 235 240 Ala Ile Phe Val Pro His Ala Asp Leu Ala Thr Thr Lys Ser Leu Val 245 250 255 Thr Ala Gly Met His Pro Ala Cys Pro Ser Arg 260 265 <210> 47 <211> 1228 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1198) <223> RXA01105 <400> 47 ttgtcatcaa ctttgccaac gccgaagatc ttccagcgca cggcgaagca atccgtgcac 60 gctttgaaaa cctccccacc accgacgagg cctaagaaaa atg acc aaa att act 115 Met Thr Lys Ile Thr 1 5 ttg agc gat ttg cca ttg cgt gaa gaa ctg cgc ggt gag cac gct tac 163 Leu Ser Asp Leu Pro Leu Arg Glu Glu Leu Arg Gly Glu His Ala Tyr 10 15 20 ggc gca ccc cag ctc aac gtt gat att cgc ctc aac acc aac gaa aac 211 Gly Ala Pro Gln Leu Asn Val Asp Ile Arg Leu Asn Thr Asn Glu Asn 25 30 35 cct tac cca ccg tca gag gca ttg gtc gct gac ttg gtt gcc acc gtg 259 Pro Tyr Pro Pro Ser Glu Ala Leu Val Ala Asp Leu Val Ala Thr Val 40 45 50 gat aag atc gcc acc gag ctg aac cgc tac cca gag cgc gat gct gtg 307 Asp Lys Ile Ala Thr Glu Leu Asn Arg Tyr Pro Glu Arg Asp Ala Val 55 60 65 gaa ctg cgt gat gag ttg gct gcg tac atc acc aag caa acc ggc gtg 355 Glu Leu Arg Asp Glu Leu Ala Ala Tyr Ile Thr Lys Gln Thr Gly Val 70 75 80 85 gct gtc acc agg gat aac ctg tgg gct gcc aat ggt tcc aat gaa att 403 Ala Val Thr Arg Asp Asn Leu Trp Ala Ala Asn Gly Ser Asn Glu Ile 90 95 100 ctg cag cag ctg ctg cag gct ttt ggt gga cct gga cgc acc gcg ttg 451 Leu Gln Gln Leu Leu Gln Ala Phe Gly Gly Pro Gly Arg Thr Ala Leu 105 110 115 gga ttc caa ccc agc tat tcc atg cac cca att ttg gct aaa ggc acc 499 Gly Phe Gln Pro Ser Tyr Ser Met His Pro Ile Leu Ala Lys Gly Thr 120 125 130 cac act gaa ttc att gcg gtg tcc cga ggt gct gat ttc cgc atc gat 547 His Thr Glu Phe Ile Ala Val Ser Arg Gly Ala Asp Phe Arg Ile Asp 135 140 145 atg gat gtg gcg ctg gaa gaa att cgt gca aag cag cct gac att gtt 595 Met Asp Val Ala Leu Glu Glu Ile Arg Ala Lys Gln Pro Asp Ile Val 150 155 160 165 ttt gtc acc acc ccg aac aac ccg acc ggt gat gtg acc tcg ctg gac 643 Phe Val Thr Thr Pro Asn Asn Pro Thr Gly Asp Val Thr Ser Leu Asp 170 175 180 gat gtt gag cgc atc atc aac gtt gcc cca ggc atc gtg atc gtg gat 691 Asp Val Glu Arg Ile Ile Asn Val Ala Pro Gly Ile Val Ile Val Asp 185 190 195 gaa gct tat gcg gaa ttc tcc cca tca cct tca gca acc act ctt ctg 739 Glu Ala Tyr Ala Glu Phe Ser Pro Ser Pro Ser Ala Thr Thr Leu Leu 200 205 210 gag aag tac cca acc aag ctg gtg gtg tcc cgc acc atg agt aag gct 787 Glu Lys Tyr Pro Thr Lys Leu Val Val Ser Arg Thr Met Ser Lys Ala 215 220 225 ttt gat ttc gca ggt gga cgc ctc ggc tac ttc gtg gcc aac cca gcg 835 Phe Asp Phe Ala Gly Gly Arg Leu Gly Tyr Phe Val Ala Asn Pro Ala 230 235 240 245 ttt atc gac gcc gtg atg cta gtc cgc ctt ccg tat cat ctt tca gcg 883 Phe Ile Asp Ala Val Met Leu Val Arg Leu Pro Tyr His Leu Ser Ala 250 255 260 ctg agc caa gca gcc gca atc gta gcg ctg cgt cac tcc gct gac acg 931 Leu Ser Gln Ala Ala Ala Ile Val Ala Leu Arg His Ser Ala Asp Thr 265 270 275 ctg gga acc gtc gaa aag ctc tct gta gag cgt gtt cgc gtg gca gca 979 Leu Gly Thr Val Glu Lys Leu Ser Val Glu Arg Val Arg Val Ala Ala 280 285 290 cgc ttg gag gaa ctg ggc tac gct gtg gtt cca agt gag tcc aac ttt 1027 Arg Leu Glu Glu Leu Gly Tyr Ala Val Val Pro Ser Glu Ser Asn Phe 295 300 305 gtg ttc ttt gga gat ttc tcc gat cag cac gcg gca tgg cag gca ttt 1075 Val Phe Phe Gly Asp Phe Ser Asp Gln His Ala Ala Trp Gln Ala Phe 310 315 320 325 ttg gat agg gga gtg ctc atc cgc gat gtg gga atc gct ggg cac ttg 1123 Leu Asp Arg Gly Val Leu Ile Arg Asp Val Gly Ile Ala Gly His Leu 330 335 340 cgc act acc att ggt gtg cct gag gaa aat gat gcg ttt ttg gac gca 1171 Arg Thr Thr Ile Gly Val Pro Glu Glu Asn Asp Ala Phe Leu Asp Ala 345 350 355 gct gca gag atc atc aag ctg aac ctg taagagagaa gaatttttca 1218 Ala Ala Glu Ile Ile Lys Leu Asn Leu 360 365 tgactgtcgc 1228 <210> 48 <211> 366 <212> PRT <213> Corynebacterium glutamicum <400> 48 Met Thr Lys Ile Thr Leu Ser Asp Leu Pro Leu Arg Glu Glu Leu Arg 1 5 10 15 Gly Glu His Ala Tyr Gly Ala Pro Gln Leu Asn Val Asp Ile Arg Leu 20 25 30 Asn Thr Asn Glu Asn Pro Tyr Pro Pro Ser Glu Ala Leu Val Ala Asp 35 40 45 Leu Val Ala Thr Val Asp Lys Ile Ala Thr Glu Leu Asn Arg Tyr Pro 50 55 60 Glu Arg Asp Ala Val Glu Leu Arg Asp Glu Leu Ala Ala Tyr Ile Thr 65 70 75 80 Lys Gln Thr Gly Val Ala Val Thr Arg Asp Asn Leu Trp Ala Ala Asn 85 90 95 Gly Ser Asn Glu Ile Leu Gln Gln Leu Leu Gln Ala Phe Gly Gly Pro 100 105 110 Gly Arg Thr Ala Leu Gly Phe Gln Pro Ser Tyr Ser Met His Pro Ile 115 120 125 Leu Ala Lys Gly Thr His Thr Glu Phe Ile Ala Val Ser Arg Gly Ala 130 135 140 Asp Phe Arg Ile Asp Met Asp Val Ala Leu Glu Glu Ile Arg Ala Lys 145 150 155 160 Gln Pro Asp Ile Val Phe Val Thr Thr Pro Asn Asn Pro Thr Gly Asp 165 170 175 Val Thr Ser Leu Asp Asp Val Glu Arg Ile Ile Asn Val Ala Pro Gly 180 185 190 Ile Val Ile Val Asp Glu Ala Tyr Ala Glu Phe Ser Pro Ser Pro Ser 195 200 205 Ala Thr Thr Leu Leu Glu Lys Tyr Pro Thr Lys Leu Val Val Ser Arg 210 215 220 Thr Met Ser Lys Ala Phe Asp Phe Ala Gly Gly Arg Leu Gly Tyr Phe 225 230 235 240 Val Ala Asn Pro Ala Phe Ile Asp Ala Val Met Leu Val Arg Leu Pro 245 250 255 Tyr His Leu Ser Ala Leu Ser Gln Ala Ala Ala Ile Val Ala Leu Arg 260 265 270 His Ser Ala Asp Thr Leu Gly Thr Val Glu Lys Leu Ser Val Glu Arg 275 280 285 Val Arg Val Ala Ala Arg Leu Glu Glu Leu Gly Tyr Ala Val Val Pro 290 295 300 Ser Glu Ser Asn Phe Val Phe Phe Gly Asp Phe Ser Asp Gln His Ala 305 310 315 320 Ala Trp Gln Ala Phe Leu Asp Arg Gly Val Leu Ile Arg Asp Val Gly 325 330 335 Ile Ala Gly His Leu Arg Thr Thr Ile Gly Val Pro Glu Glu Asn Asp 340 345 350 Ala Phe Leu Asp Ala Ala Ala Glu Ile Ile Lys Leu Asn Leu 355 360 365 <210> 49 <211> 883 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(853) <223> RXA01115 <400> 49 ctcaccgcat gcagcgtgaa aacactcagt acggactggc caccatgtgc atcggtggcg 60 gccagggtct tgcagctgtc tttgaaaagg agaactaaaa atg gct att ttg cac 115 Met Ala Ile Leu His 1 5 agc gtt tcc tac gga act tcc gac aac acc ttg gtg ttc att ggc tcg 163 Ser Val Ser Tyr Gly Thr Ser Asp Asn Thr Leu Val Phe Ile Gly Ser 10 15 20 ttg ggt tcc acc acc gac atg tgg ctg cca cag ctg gat gcc ttg cat 211 Leu Gly Ser Thr Thr Asp Met Trp Leu Pro Gln Leu Asp Ala Leu His 25 30 35 aag gat ttc cgc gtc atc gct gtt gat cac cgc gga cat ggt ctg tct 259 Lys Asp Phe Arg Val Ile Ala Val Asp His Arg Gly His Gly Leu Ser 40 45 50 gaa ctc atc gaa ggc acc ccc act gtg gcg gat ctg gcg cag gat gtg 307 Glu Leu Ile Glu Gly Thr Pro Thr Val Ala Asp Leu Ala Gln Asp Val 55 60 65 ctg gat acc ctc gat gac ctg ggt gtc gga aac ttc ggc gtc atc gga 355 Leu Asp Thr Leu Asp Asp Leu Gly Val Gly Asn Phe Gly Val Ile Gly 70 75 80 85 cta tct ctc ggc gga gcg gtt gca caa tac ttg gcg gcc acc tct gat 403 Leu Ser Leu Gly Gly Ala Val Ala Gln Tyr Leu Ala Ala Thr Ser Asp 90 95 100 cgt gtc acc aag gca gca ttc atg tgt acc gct gca aaa ttc ggc gag 451 Arg Val Thr Lys Ala Ala Phe Met Cys Thr Ala Ala Lys Phe Gly Glu 105 110 115 ccc cag ggc tgg cta gat cgc gcc gca gcg tgc cgc gaa aac ggc act 499 Pro Gln Gly Trp Leu Asp Arg Ala Ala Ala Cys Arg Glu Asn Gly Thr 120 125 130 ggt tct ctg tcc gaa gct gtg atc cag cgc tgg ttc tcc ccc act tgg 547 Gly Ser Leu Ser Glu Ala Val Ile Gln Arg Trp Phe Ser Pro Thr Trp 135 140 145 ttg gag aac aac cca gcg tcc cgc gag cac ttc gaa gcc atg gtt gcc 595 Leu Glu Asn Asn Pro Ala Ser Arg Glu His Phe Glu Ala Met Val Ala 150 155 160 165 ggc acc cca tct gag ggt tac gcg ctg tgc tgc gag gcg ttg gca acc 643 Gly Thr Pro Ser Glu Gly Tyr Ala Leu Cys Cys Glu Ala Leu Ala Thr 170 175 180 tgg gat ttc acc gat cgc ctg gga gaa atc acc gtg cca gtg ctc acc 691 Trp Asp Phe Thr Asp Arg Leu Gly Glu Ile Thr Val Pro Val Leu Thr 185 190 195 atc gca ggt gcc gat gac ccc tcc act cct cca gca acc gtg cag atc 739 Ile Ala Gly Ala Asp Asp Pro Ser Thr Pro Pro Ala Thr Val Gln Ile 200 205 210 att gcc gat ggc gtt ggc ggc gag tcc cgc gca gag gtc cta agc cca 787 Ile Ala Asp Gly Val Gly Gly Glu Ser Arg Ala Glu Val Leu Ser Pro 215 220 225 gcc gcg cac gta cca acc gtg gaa cgt cca aac gag gta aat gaa ctg 835 Ala Ala His Val Pro Thr Val Glu Arg Pro Asn Glu Val Asn Glu Leu 230 235 240 245 cta gca cag cat ttc gct taatgttgta ggcatgttca caaccagtct 883 Leu Ala Gln His Phe Ala 250 <210> 50 <211> 251 <212> PRT <213> Corynebacterium glutamicum <400> 50 Met Ala Ile Leu His Ser Val Ser Tyr Gly Thr Ser Asp Asn Thr Leu 1 5 10 15 Val Phe Ile Gly Ser Leu Gly Ser Thr Thr Asp Met Trp Leu Pro Gln 20 25 30 Leu Asp Ala Leu His Lys Asp Phe Arg Val Ile Ala Val Asp His Arg 35 40 45 Gly His Gly Leu Ser Glu Leu Ile Glu Gly Thr Pro Thr Val Ala Asp 50 55 60 Leu Ala Gln Asp Val Leu Asp Thr Leu Asp Asp Leu Gly Val Gly Asn 65 70 75 80 Phe Gly Val Ile Gly Leu Ser Leu Gly Gly Ala Val Ala Gln Tyr Leu 85 90 95 Ala Ala Thr Ser Asp Arg Val Thr Lys Ala Ala Phe Met Cys Thr Ala 100 105 110 Ala Lys Phe Gly Glu Pro Gln Gly Trp Leu Asp Arg Ala Ala Ala Cys 115 120 125 Arg Glu Asn Gly Thr Gly Ser Leu Ser Glu Ala Val Ile Gln Arg Trp 130 135 140 Phe Ser Pro Thr Trp Leu Glu Asn Asn Pro Ala Ser Arg Glu His Phe 145 150 155 160 Glu Ala Met Val Ala Gly Thr Pro Ser Glu Gly Tyr Ala Leu Cys Cys 165 170 175 Glu Ala Leu Ala Thr Trp Asp Phe Thr Asp Arg Leu Gly Glu Ile Thr 180 185 190 Val Pro Val Leu Thr Ile Ala Gly Ala Asp Asp Pro Ser Thr Pro Pro 195 200 205 Ala Thr Val Gln Ile Ile Ala Asp Gly Val Gly Gly Glu Ser Arg Ala 210 215 220 Glu Val Leu Ser Pro Ala Ala His Val Pro Thr Val Glu Arg Pro Asn 225 230 235 240 Glu Val Asn Glu Leu Leu Ala Gln His Phe Ala 245 250 <210> 51 <211> 1171 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1141) <223> RXA01127 <400> 51 aacgatttcc agttgagtca agccctgcat gatggggtag tgggggttgt tgggcaggta 60 cgagctgtga tcaatcagct acactagtga agtccatata gtg aga agg gaa tcc 115 Val Arg Arg Glu Ser 1 5 cac aac atg aaa ctt gct gtt att ggt gga gat ggt atc ggc cca gag 163 His Asn Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu 10 15 20 gtt act gca gaa gcc ctc aag gtt cta aac gct gtc cgc gac gac atc 211 Val Thr Ala Glu Ala Leu Lys Val Leu Asn Ala Val Arg Asp Asp Ile 25 30 35 gag acc acc gat tat gac ctt ggc gca cgc cgt tac ctc aaa aat ggc 259 Glu Thr Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Lys Asn Gly 40 45 50 gag ctg ctc acc gac gag gat ctg gca tcc ctg cgc gag cat gac gcg 307 Glu Leu Leu Thr Asp Glu Asp Leu Ala Ser Leu Arg Glu His Asp Ala 55 60 65 atc ctt ctt ggc gct atc ggt gca cca ggt tcc gtc cct cca gga att 355 Ile Leu Leu Gly Ala Ile Gly Ala Pro Gly Ser Val Pro Pro Gly Ile 70 75 80 85 ctc gag cgt ggt ttg ctg ctg aag atg cga ttc gca ctg gat cac cac 403 Leu Glu Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His 90 95 100 gtg aac ctg cgc cca tcc aag ctg tac gac ggc gtg gag tcc cca ctg 451 Val Asn Leu Arg Pro Ser Lys Leu Tyr Asp Gly Val Glu Ser Pro Leu 105 110 115 cgt aac cca ggc aag att gat ttc gtt gtg gtc cgc gaa ggt acc gaa 499 Arg Asn Pro Gly Lys Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu 120 125 130 ggc gcc tac act ggc aac ggt gga gca atc cgc gtg gga acc cct cac 547 Gly Ala Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His 135 140 145 gag att gcc aat gaa acc tcc gtg aac act cgc tac ggc gct gag cgc 595 Glu Ile Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg 150 155 160 165 gtt att cgc tac gca ttc gag ctg gca cag agc cgc cgc aag aag ctc 643 Val Ile Arg Tyr Ala Phe Glu Leu Ala Gln Ser Arg Arg Lys Lys Leu 170 175 180 acc ctc gtg cac aag acc aac gtc ctg gtt cac ggt ggt ggc ctg tgg 691 Thr Leu Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Leu Trp 185 190 195 cag cgc acc gta gat gag gtt gca aag gaa tac cca gag gta gcc gtc 739 Gln Arg Thr Val Asp Glu Val Ala Lys Glu Tyr Pro Glu Val Ala Val 200 205 210 gat tac aac cac atc gat gca gca acc atc tat ctg gtc act gat cct 787 Asp Tyr Asn His Ile Asp Ala Ala Thr Ile Tyr Leu Val Thr Asp Pro 215 220 225 tcc cgc ttc gat gtg att gtt acc gat aac ctc ttc ggc gac atc ctc 835 Ser Arg Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Leu 230 235 240 245 acc gat gag gca ggc gca gtc tct ggc gga att ggc ctc gca gca tcc 883 Thr Asp Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser 250 255 260 ggc aac atc gat gcc acg ggc acc aac cct tcc atg ttc gag cca gtc 931 Gly Asn Ile Asp Ala Thr Gly Thr Asn Pro Ser Met Phe Glu Pro Val 265 270 275 cac ggc tct gca cca gat atc gca ggc cag gga atc gca gac cca acg 979 His Gly Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr 280 285 290 gca gca atc cta tcc gct gcg atg ctg ctg cgt cac tta ggt gat gaa 1027 Ala Ala Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu 295 300 305 gac aac gca gta cgt att gaa aca gcc atc gca gct gat gtg gct ggc 1075 Asp Asn Ala Val Arg Ile Glu Thr Ala Ile Ala Ala Asp Val Ala Gly 310 315 320 325 cga gat aac tct cag ccg att tct acc act gag gtg gga gac cgc atc 1123 Arg Asp Asn Ser Gln Pro Ile Ser Thr Thr Glu Val Gly Asp Arg Ile 330 335 340 gtc aag gcg ctg caa agc taaatttcaa cgccgacccc cttgcctttt 1171 Val Lys Ala Leu Gln Ser 345 <210> 52 <211> 347 <212> PRT <213> Corynebacterium glutamicum <400> 52 Val Arg Arg Glu Ser His Asn Met Lys Leu Ala Val Ile Gly Gly Asp 1 5 10 15 Gly Ile Gly Pro Glu Val Thr Ala Glu Ala Leu Lys Val Leu Asn Ala 20 25 30 Val Arg Asp Asp Ile Glu Thr Thr Asp Tyr Asp Leu Gly Ala Arg Arg 35 40 45 Tyr Leu Lys Asn Gly Glu Leu Leu Thr Asp Glu Asp Leu Ala Ser Leu 50 55 60 Arg Glu His Asp Ala Ile Leu Leu Gly Ala Ile Gly Ala Pro Gly Ser 65 70 75 80 Val Pro Pro Gly Ile Leu Glu Arg Gly Leu Leu Leu Lys Met Arg Phe 85 90 95 Ala Leu Asp His His Val Asn Leu Arg Pro Ser Lys Leu Tyr Asp Gly 100 105 110 Val Glu Ser Pro Leu Arg Asn Pro Gly Lys Ile Asp Phe Val Val Val 115 120 125 Arg Glu Gly Thr Glu Gly Ala Tyr Thr Gly Asn Gly Gly Ala Ile Arg 130 135 140 Val Gly Thr Pro His Glu Ile Ala Asn Glu Thr Ser Val Asn Thr Arg 145 150 155 160 Tyr Gly Ala Glu Arg Val Ile Arg Tyr Ala Phe Glu Leu Ala Gln Ser 165 170 175 Arg Arg Lys Lys Leu Thr Leu Val His Lys Thr Asn Val Leu Val His 180 185 190 Gly Gly Gly Leu Trp Gln Arg Thr Val Asp Glu Val Ala Lys Glu Tyr 195 200 205 Pro Glu Val Ala Val Asp Tyr Asn His Ile Asp Ala Ala Thr Ile Tyr 210 215 220 Leu Val Thr Asp Pro Ser Arg Phe Asp Val Ile Val Thr Asp Asn Leu 225 230 235 240 Phe Gly Asp Ile Leu Thr Asp Glu Ala Gly Ala Val Ser Gly Gly Ile 245 250 255 Gly Leu Ala Ala Ser Gly Asn Ile Asp Ala Thr Gly Thr Asn Pro Ser 260 265 270 Met Phe Glu Pro Val His Gly Ser Ala Pro Asp Ile Ala Gly Gln Gly 275 280 285 Ile Ala Asp Pro Thr Ala Ala Ile Leu Ser Ala Ala Met Leu Leu Arg 290 295 300 His Leu Gly Asp Glu Asp Asn Ala Val Arg Ile Glu Thr Ala Ile Ala 305 310 315 320 Ala Asp Val Ala Gly Arg Asp Asn Ser Gln Pro Ile Ser Thr Thr Glu 325 330 335 Val Gly Asp Arg Ile Val Lys Ala Leu Gln Ser 340 345 <210> 53 <211> 1153 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1123) <223> RXA01240 <400> 53 ttgttctcac atctacccgt atctttgttg gttttagtac ccgatagtga gttttgatcc 60 ctgcacagga aagttagcgg cgctactatg aacgatcgat atg tct gac aac act 115 Met Ser Asp Asn Thr 1 5 ctc tcc caa ttt ggc agt tac tac cac gaa ttc cga cgt gcc cat ccc 163 Leu Ser Gln Phe Gly Ser Tyr Tyr His Glu Phe Arg Arg Ala His Pro 10 15 20 atg gcc gac gtc gaa ttc ctc cta gca att gaa gaa tta ctt acg gac 211 Met Ala Asp Val Glu Phe Leu Leu Ala Ile Glu Glu Leu Leu Thr Asp 25 30 35 ggt ggt gtc acc ttc gat cgc gtc acc aca cgc atc aaa gaa tgg tca 259 Gly Gly Val Thr Phe Asp Arg Val Thr Thr Arg Ile Lys Glu Trp Ser 40 45 50 agc ctg aaa gcc aag gct cgc aag cgt cgc aac gat ggc tcg ttg atc 307 Ser Leu Lys Ala Lys Ala Arg Lys Arg Arg Asn Asp Gly Ser Leu Ile 55 60 65 tac cct gat ccg cgc aaa gac atc cac gac atg atc ggt gtt cgg atc 355 Tyr Pro Asp Pro Arg Lys Asp Ile His Asp Met Ile Gly Val Arg Ile 70 75 80 85 acc acg tac cac tcc acg gaa ata ccc gtg gcc cta aaa gtg ctc caa 403 Thr Thr Tyr His Ser Thr Glu Ile Pro Val Ala Leu Lys Val Leu Gln 90 95 100 gac tcc ttc atc gtc cac aaa tcc gta gac aaa gcc gct gaa act cgc 451 Asp Ser Phe Ile Val His Lys Ser Val Asp Lys Ala Ala Glu Thr Arg 105 110 115 atc tca ggc ggc ttt ggt tac ggc tcc cac cac ctg att ctg gaa gtc 499 Ile Ser Gly Gly Phe Gly Tyr Gly Ser His His Leu Ile Leu Glu Val 120 125 130 gat gac acc tcc gat gac ctc cag gac tac aaa ggc ctc gtc ttt gaa 547 Asp Asp Thr Ser Asp Asp Leu Gln Asp Tyr Lys Gly Leu Val Phe Glu 135 140 145 gtt cag gtg cgc acc gtg ctg caa cac gcc tgg gca gag ttc gaa cac 595 Val Gln Val Arg Thr Val Leu Gln His Ala Trp Ala Glu Phe Glu His 150 155 160 165 gat atc cgc tat aaa cgc gcc gat gtg tcc aac cca gaa gac ttc agc 643 Asp Ile Arg Tyr Lys Arg Ala Asp Val Ser Asn Pro Glu Asp Phe Ser 170 175 180 gca gaa gta gac cgc atg ttc acc ctc gct gcc gga ctc atc gaa tta 691 Ala Glu Val Asp Arg Met Phe Thr Leu Ala Ala Gly Leu Ile Glu Leu 185 190 195 gcg gac caa caa ttc gac caa atc gcc gca ctc aaa gaa acc agc cga 739 Ala Asp Gln Gln Phe Asp Gln Ile Ala Ala Leu Lys Glu Thr Ser Arg 200 205 210 gtt gct gat gaa tcc gtc gaa ctc acc gca gag aca ctt ccc ggc gtt 787 Val Ala Asp Glu Ser Val Glu Leu Thr Ala Glu Thr Leu Pro Gly Val 215 220 225 ctt gcc atg ctc att ggc aac cgc ttc ccc cgc cca cgc tcc aca aac 835 Leu Ala Met Leu Ile Gly Asn Arg Phe Pro Arg Pro Arg Ser Thr Asn 230 235 240 245 tac cgc ttc ctc gaa gac atc ctg gtg gcc aac tcc att acc tct gtg 883 Tyr Arg Phe Leu Glu Asp Ile Leu Val Ala Asn Ser Ile Thr Ser Val 250 255 260 gtg cag ctg cgc gag ctg ctc aac ccc acc gac att gaa gtg ctg ttg 931 Val Gln Leu Arg Glu Leu Leu Asn Pro Thr Asp Ile Glu Val Leu Leu 265 270 275 aaa gta atg aac tac cgc ttc cac cca ggc cag atc cgc atc atc gac 979 Lys Val Met Asn Tyr Arg Phe His Pro Gly Gln Ile Arg Ile Ile Asp 280 285 290 gat tta cta ctc aaa cga ttt ggc caa tca cac atc gat gcc acc gtc 1027 Asp Leu Leu Leu Lys Arg Phe Gly Gln Ser His Ile Asp Ala Thr Val 295 300 305 gcc acc gac tcc caa cca ctc aac gcc aaa cgc cac agg cag cta aaa 1075 Ala Thr Asp Ser Gln Pro Leu Asn Ala Lys Arg His Arg Gln Leu Lys 310 315 320 325 cgc aag cta gag ctc atg acc caa gct cat ctt gtg gaa cca cca aac 1123 Arg Lys Leu Glu Leu Met Thr Gln Ala His Leu Val Glu Pro Pro Asn 330 335 340 taaagttccc ccaaagttag ccccggctaa 1153 <210> 54 <211> 341 <212> PRT <213> Corynebacterium glutamicum <400> 54 Met Ser Asp Asn Thr Leu Ser Gln Phe Gly Ser Tyr Tyr His Glu Phe 1 5 10 15 Arg Arg Ala His Pro Met Ala Asp Val Glu Phe Leu Leu Ala Ile Glu 20 25 30 Glu Leu Leu Thr Asp Gly Gly Val Thr Phe Asp Arg Val Thr Thr Arg 35 40 45 Ile Lys Glu Trp Ser Ser Leu Lys Ala Lys Ala Arg Lys Arg Arg Asn 50 55 60 Asp Gly Ser Leu Ile Tyr Pro Asp Pro Arg Lys Asp Ile His Asp Met 65 70 75 80 Ile Gly Val Arg Ile Thr Thr Tyr His Ser Thr Glu Ile Pro Val Ala 85 90 95 Leu Lys Val Leu Gln Asp Ser Phe Ile Val His Lys Ser Val Asp Lys 100 105 110 Ala Ala Glu Thr Arg Ile Ser Gly Gly Phe Gly Tyr Gly Ser His His 115 120 125 Leu Ile Leu Glu Val Asp Asp Thr Ser Asp Asp Leu Gln Asp Tyr Lys 130 135 140 Gly Leu Val Phe Glu Val Gln Val Arg Thr Val Leu Gln His Ala Trp 145 150 155 160 Ala Glu Phe Glu His Asp Ile Arg Tyr Lys Arg Ala Asp Val Ser Asn 165 170 175 Pro Glu Asp Phe Ser Ala Glu Val Asp Arg Met Phe Thr Leu Ala Ala 180 185 190 Gly Leu Ile Glu Leu Ala Asp Gln Gln Phe Asp Gln Ile Ala Ala Leu 195 200 205 Lys Glu Thr Ser Arg Val Ala Asp Glu Ser Val Glu Leu Thr Ala Glu 210 215 220 Thr Leu Pro Gly Val Leu Ala Met Leu Ile Gly Asn Arg Phe Pro Arg 225 230 235 240 Pro Arg Ser Thr Asn Tyr Arg Phe Leu Glu Asp Ile Leu Val Ala Asn 245 250 255 Ser Ile Thr Ser Val Val Gln Leu Arg Glu Leu Leu Asn Pro Thr Asp 260 265 270 Ile Glu Val Leu Leu Lys Val Met Asn Tyr Arg Phe His Pro Gly Gln 275 280 285 Ile Arg Ile Ile Asp Asp Leu Leu Leu Lys Arg Phe Gly Gln Ser His 290 295 300 Ile Asp Ala Thr Val Ala Thr Asp Ser Gln Pro Leu Asn Ala Lys Arg 305 310 315 320 His Arg Gln Leu Lys Arg Lys Leu Glu Leu Met Thr Gln Ala His Leu 325 330 335 Val Glu Pro Pro Asn 340 <210> 55 <211> 1105 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1075) <223> RXA01253 <400> 55 caggctggca agaagccctc tccatggttg atcgcacagc tgatggctta gtcatcgtcg 60 tcaatggcca ggttgccgac ggcgaagacc tctcctggct ttg gga cgt ccg ctt 115 Leu Gly Arg Pro Leu 1 5 cga aga ctt cga aaa cct ctc cgt caa agc ctc cgg cga gcg cgg cac 163 Arg Arg Leu Arg Lys Pro Leu Arg Gln Ser Leu Arg Arg Ala Arg His 10 15 20 cga cct ggc agt ccg cct cac cta cgc cga aat cga cca cga act cat 211 Arg Pro Gly Ser Pro Pro His Leu Arg Arg Asn Arg Pro Arg Thr His 25 30 35 ctc caa ccc cgt cga cgc cat cgc agc ctg ccc tcc tgg ccg cat cga 259 Leu Gln Pro Arg Arg Arg His Arg Ser Leu Pro Ser Trp Pro His Arg 40 45 50 agt cct cgc caa cta cac cgc att ccg aga cct caa aaa ggc tct gga 307 Ser Pro Arg Gln Leu His Arg Ile Pro Arg Pro Gln Lys Gly Ser Gly 55 60 65 gaa agg gac cga aca ata atg acc acc ctc aac atc ggc ctc atc ctc 355 Glu Arg Asp Arg Thr Ile Met Thr Thr Leu Asn Ile Gly Leu Ile Leu 70 75 80 85 ccc gac gtc ctc gga act tac ggc gac gac ggc aac gca cta gtc ctg 403 Pro Asp Val Leu Gly Thr Tyr Gly Asp Asp Gly Asn Ala Leu Val Leu 90 95 100 cgc caa cgc gca cgc atg cgt ggc att aat gct gaa atc cag cgc gtc 451 Arg Gln Arg Ala Arg Met Arg Gly Ile Asn Ala Glu Ile Gln Arg Val 105 110 115 acc ctc gac gac gcc gtc cct tcc acc ctt gat ctc tac tgc ctc ggc 499 Thr Leu Asp Asp Ala Val Pro Ser Thr Leu Asp Leu Tyr Cys Leu Gly 120 125 130 ggc ggc gag gac acc gca cag atc ctt gcc acc gaa cac ctc acc aaa 547 Gly Gly Glu Asp Thr Ala Gln Ile Leu Ala Thr Glu His Leu Thr Lys 135 140 145 gac ggc ggc ctc caa acc gca gcc gcc gca ggc cgc ccc atc ttc gca 595 Asp Gly Gly Leu Gln Thr Ala Ala Ala Ala Gly Arg Pro Ile Phe Ala 150 155 160 165 gtc tgc gca ggt ctc cag gta ctc ggc gac tcc ttc cgc gcc gcc ggc 643 Val Cys Ala Gly Leu Gln Val Leu Gly Asp Ser Phe Arg Ala Ala Gly 170 175 180 cgt gtc atc gac ggc ctt ggg ctt atc gac gcc acc acc gtc tct tta 691 Arg Val Ile Asp Gly Leu Gly Leu Ile Asp Ala Thr Thr Val Ser Leu 185 190 195 caa aaa cgc gcc atc gga gaa gtc gaa acg aca cca acc cgc gcc gga 739 Gln Lys Arg Ala Ile Gly Glu Val Glu Thr Thr Pro Thr Arg Ala Gly 200 205 210 ttc acc gcc gag ctg acc gaa cga ctc acc ggc ttc gaa aac cac atg 787 Phe Thr Ala Glu Leu Thr Glu Arg Leu Thr Gly Phe Glu Asn His Met 215 220 225 ggc gcc acc ctg ctc ggc ccc gac gcc gaa cca ctc ggc cga gtc gtc 835 Gly Ala Thr Leu Leu Gly Pro Asp Ala Glu Pro Leu Gly Arg Val Val 230 235 240 245 cgc ggc gaa ggc aac acc gat gtc tgg gca gcc tcc gaa aac acc gac 883 Arg Gly Glu Gly Asn Thr Asp Val Trp Ala Ala Ser Glu Asn Thr Asp 250 255 260 gac caa cgc caa caa ttc gcc gaa ggc gcc gtc caa ggc agc atc atc 931 Asp Gln Arg Gln Gln Phe Ala Glu Gly Ala Val Gln Gly Ser Ile Ile 265 270 275 gcc acc tac atg cac ggc ccc gca ctc gcc cga aac ccc caa ctc gcc 979 Ala Thr Tyr Met His Gly Pro Ala Leu Ala Arg Asn Pro Gln Leu Ala 280 285 290 gac ctc atg ctc gca aaa gca atg ggt gtc gcg ctg aaa gac ctg gag 1027 Asp Leu Met Leu Ala Lys Ala Met Gly Val Ala Leu Lys Asp Leu Glu 295 300 305 cct ttg gac atc gac gtc atc gac cgc ctc cgc gcc gaa cgc ctg gcg 1075 Pro Leu Asp Ile Asp Val Ile Asp Arg Leu Arg Ala Glu Arg Leu Ala 310 315 320 325 tagccccttc taaaccgggt ctactcgaaa 1105 <210> 56 <211> 325 <212> PRT <213> Corynebacterium glutamicum <400> 56 Leu Gly Arg Pro Leu Arg Arg Leu Arg Lys Pro Leu Arg Gln Ser Leu 1 5 10 15 Arg Arg Ala Arg His Arg Pro Gly Ser Pro Pro His Leu Arg Arg Asn 20 25 30 Arg Pro Arg Thr His Leu Gln Pro Arg Arg Arg His Arg Ser Leu Pro 35 40 45 Ser Trp Pro His Arg Ser Pro Arg Gln Leu His Arg Ile Pro Arg Pro 50 55 60 Gln Lys Gly Ser Gly Glu Arg Asp Arg Thr Ile Met Thr Thr Leu Asn 65 70 75 80 Ile Gly Leu Ile Leu Pro Asp Val Leu Gly Thr Tyr Gly Asp Asp Gly 85 90 95 Asn Ala Leu Val Leu Arg Gln Arg Ala Arg Met Arg Gly Ile Asn Ala 100 105 110 Glu Ile Gln Arg Val Thr Leu Asp Asp Ala Val Pro Ser Thr Leu Asp 115 120 125 Leu Tyr Cys Leu Gly Gly Gly Glu Asp Thr Ala Gln Ile Leu Ala Thr 130 135 140 Glu His Leu Thr Lys Asp Gly Gly Leu Gln Thr Ala Ala Ala Ala Gly 145 150 155 160 Arg Pro Ile Phe Ala Val Cys Ala Gly Leu Gln Val Leu Gly Asp Ser 165 170 175 Phe Arg Ala Ala Gly Arg Val Ile Asp Gly Leu Gly Leu Ile Asp Ala 180 185 190 Thr Thr Val Ser Leu Gln Lys Arg Ala Ile Gly Glu Val Glu Thr Thr 195 200 205 Pro Thr Arg Ala Gly Phe Thr Ala Glu Leu Thr Glu Arg Leu Thr Gly 210 215 220 Phe Glu Asn His Met Gly Ala Thr Leu Leu Gly Pro Asp Ala Glu Pro 225 230 235 240 Leu Gly Arg Val Val Arg Gly Glu Gly Asn Thr Asp Val Trp Ala Ala 245 250 255 Ser Glu Asn Thr Asp Asp Gln Arg Gln Gln Phe Ala Glu Gly Ala Val 260 265 270 Gln Gly Ser Ile Ile Ala Thr Tyr Met His Gly Pro Ala Leu Ala Arg 275 280 285 Asn Pro Gln Leu Ala Asp Leu Met Leu Ala Lys Ala Met Gly Val Ala 290 295 300 Leu Lys Asp Leu Glu Pro Leu Asp Ile Asp Val Ile Asp Arg Leu Arg 305 310 315 320 Ala Glu Arg Leu Ala 325 <210> 57 <211> 1132 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1102) <223> RXA01321 <400> 57 ggacacagat cgtgccgact cccagctcaa ttgcggaatc tataaatgcg gccagttcgg 60 agttggttac ttctggtccg aggagggtgt agtcaaggat ttg ggc cat ggt gga 115 Leu Gly His Gly Gly 1 5 gcg gga aat cgt cat atc cat acc cta ctt aga cct gac tta gtg tgg 163 Ala Gly Asn Arg His Ile His Thr Leu Leu Arg Pro Asp Leu Val Trp 10 15 20 gaa aat ttc cag ggt aga atg caa cga atg acc ccg agt tct cct gaa 211 Glu Asn Phe Gln Gly Arg Met Gln Arg Met Thr Pro Ser Ser Pro Glu 25 30 35 gtt cgt aat cgt ccg agc gct gcg cct gaa gag cgt cag ttt gtg ctc 259 Val Arg Asn Arg Pro Ser Ala Ala Pro Glu Glu Arg Gln Phe Val Leu 40 45 50 act ttt ggc tgc cct gac tcc act gga att gtg gcg aag ttg tcg tcg 307 Thr Phe Gly Cys Pro Asp Ser Thr Gly Ile Val Ala Lys Leu Ser Ser 55 60 65 ttc cta gct gag cgt ggg ggt tgg att act gag gct gga tat ttc acg 355 Phe Leu Ala Glu Arg Gly Gly Trp Ile Thr Glu Ala Gly Tyr Phe Thr 70 75 80 85 gat cct gat tcg aat tgg ttc ttt act cgt cag gcg att cgc gct gag 403 Asp Pro Asp Ser Asn Trp Phe Phe Thr Arg Gln Ala Ile Arg Ala Glu 90 95 100 tcg att gat acc acg att gag cag ttg cgg gag gag ttc gct ccg ctt 451 Ser Ile Asp Thr Thr Ile Glu Gln Leu Arg Glu Glu Phe Ala Pro Leu 105 110 115 gcg gag gag ttc ggc ccg agg gct aag tgg agt ttc act gac act gcg 499 Ala Glu Glu Phe Gly Pro Arg Ala Lys Trp Ser Phe Thr Asp Thr Ala 120 125 130 cag gtg aag aag gct gtg ttg ttg gtg tct aag gag ggc cac tgc ttg 547 Gln Val Lys Lys Ala Val Leu Leu Val Ser Lys Glu Gly His Cys Leu 135 140 145 cac gat ttg tta ggt cgt gtg gct gag aat gat tat ccg atg gaa gtt 595 His Asp Leu Leu Gly Arg Val Ala Glu Asn Asp Tyr Pro Met Glu Val 150 155 160 165 gtt gcg gtt gtg ggt aac cat gag aac ttg cgt tat att gcg gag aac 643 Val Ala Val Val Gly Asn His Glu Asn Leu Arg Tyr Ile Ala Glu Asn 170 175 180 cat aat gtt ccg ttt ttc cat gtg ccg ttt cct aag gat gcg gtt ggt 691 His Asn Val Pro Phe Phe His Val Pro Phe Pro Lys Asp Ala Val Gly 185 190 195 aag cgg aag gcg ttt gac cag gtc gct gag att gtg aat ggt tat gat 739 Lys Arg Lys Ala Phe Asp Gln Val Ala Glu Ile Val Asn Gly Tyr Asp 200 205 210 ccg gat gcg att gtt ttg gct cgt ttt atg cag att ttg ccg ccg gat 787 Pro Asp Ala Ile Val Leu Ala Arg Phe Met Gln Ile Leu Pro Pro Asp 215 220 225 ttg tgt gag atg tgg gct ggt cgt gtg ttg aat att cat cac agt ttc 835 Leu Cys Glu Met Trp Ala Gly Arg Val Leu Asn Ile His His Ser Phe 230 235 240 245 ttg ccg tcg ttt atg ggt gcg cgc ccg tat cat cag gcg tat agc cgt 883 Leu Pro Ser Phe Met Gly Ala Arg Pro Tyr His Gln Ala Tyr Ser Arg 250 255 260 ggt gtg aag ttg att ggt gcg acc tgc cat tat gcg act ggg gat ctg 931 Gly Val Lys Leu Ile Gly Ala Thr Cys His Tyr Ala Thr Gly Asp Leu 265 270 275 gat gat ggt ccg atc att gag cag gat gtt att cgt gtg acg cat aag 979 Asp Asp Gly Pro Ile Ile Glu Gln Asp Val Ile Arg Val Thr His Lys 280 285 290 gat acg ccg act gag atg cag cgt ttg ggc cgc gat gcg gag aag cag 1027 Asp Thr Pro Thr Glu Met Gln Arg Leu Gly Arg Asp Ala Glu Lys Gln 295 300 305 gtg ctg gct cgc ggt ttg cgt ttc cac ttg gag gac cgg gtg ctg gtt 1075 Val Leu Ala Arg Gly Leu Arg Phe His Leu Glu Asp Arg Val Leu Val 310 315 320 325 tac ggt aac cgc acg gtt gtc ttt gat taaggctttt tgcttttcga 1122 Tyr Gly Asn Arg Thr Val Val Phe Asp 330 cgcgacccta 1132 <210> 58 <211> 334 <212> PRT <213> Corynebacterium glutamicum <400> 58 Leu Gly His Gly Gly Ala Gly Asn Arg His Ile His Thr Leu Leu Arg 1 5 10 15 Pro Asp Leu Val Trp Glu Asn Phe Gln Gly Arg Met Gln Arg Met Thr 20 25 30 Pro Ser Ser Pro Glu Val Arg Asn Arg Pro Ser Ala Ala Pro Glu Glu 35 40 45 Arg Gln Phe Val Leu Thr Phe Gly Cys Pro Asp Ser Thr Gly Ile Val 50 55 60 Ala Lys Leu Ser Ser Phe Leu Ala Glu Arg Gly Gly Trp Ile Thr Glu 65 70 75 80 Ala Gly Tyr Phe Thr Asp Pro Asp Ser Asn Trp Phe Phe Thr Arg Gln 85 90 95 Ala Ile Arg Ala Glu Ser Ile Asp Thr Thr Ile Glu Gln Leu Arg Glu 100 105 110 Glu Phe Ala Pro Leu Ala Glu Glu Phe Gly Pro Arg Ala Lys Trp Ser 115 120 125 Phe Thr Asp Thr Ala Gln Val Lys Lys Ala Val Leu Leu Val Ser Lys 130 135 140 Glu Gly His Cys Leu His Asp Leu Leu Gly Arg Val Ala Glu Asn Asp 145 150 155 160 Tyr Pro Met Glu Val Val Ala Val Val Gly Asn His Glu Asn Leu Arg 165 170 175 Tyr Ile Ala Glu Asn His Asn Val Pro Phe Phe His Val Pro Phe Pro 180 185 190 Lys Asp Ala Val Gly Lys Arg Lys Ala Phe Asp Gln Val Ala Glu Ile 195 200 205 Val Asn Gly Tyr Asp Pro Asp Ala Ile Val Leu Ala Arg Phe Met Gln 210 215 220 Ile Leu Pro Pro Asp Leu Cys Glu Met Trp Ala Gly Arg Val Leu Asn 225 230 235 240 Ile His His Ser Phe Leu Pro Ser Phe Met Gly Ala Arg Pro Tyr His 245 250 255 Gln Ala Tyr Ser Arg Gly Val Lys Leu Ile Gly Ala Thr Cys His Tyr 260 265 270 Ala Thr Gly Asp Leu Asp Asp Gly Pro Ile Ile Glu Gln Asp Val Ile 275 280 285 Arg Val Thr His Lys Asp Thr Pro Thr Glu Met Gln Arg Leu Gly Arg 290 295 300 Asp Ala Glu Lys Gln Val Leu Ala Arg Gly Leu Arg Phe His Leu Glu 305 310 315 320 Asp Arg Val Leu Val Tyr Gly Asn Arg Thr Val Val Phe Asp 325 330 <210> 59 <211> 1243 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1213) <223> RXA01381 <400> 59 gcgatggccg aatccatgtt ccacgccgtt gaagccggaa gattagccgc ccaagcaggc 60 cggatcccgc aacgccaaca tgcggtggcc tcatcaagtt ttg aag gtt tgg cat 115 Leu Lys Val Trp His 1 5 cct ggg cgg agc agg tgc ttt aac atg gcg cag ctt ccc aca tct gag 163 Pro Gly Arg Ser Arg Cys Phe Asn Met Ala Gln Leu Pro Thr Ser Glu 10 15 20 ctt cac cgc acg gcc cgc caa ctc gcg ctc ccc gga tat ggt att gag 211 Leu His Arg Thr Ala Arg Gln Leu Ala Leu Pro Gly Tyr Gly Ile Glu 25 30 35 cag cag gaa cga ctt ttc aac gcc cac gtt cta gtc atc ggc gca ggc 259 Gln Gln Glu Arg Leu Phe Asn Ala His Val Leu Val Ile Gly Ala Gly 40 45 50 ggt ctc ggc tgc cca gtc atg cag tcg ctg gct tcc gca ggc gtt gga 307 Gly Leu Gly Cys Pro Val Met Gln Ser Leu Ala Ser Ala Gly Val Gly 55 60 65 acc atc acg gtc atc gat gac gac acc gtc gac att tcc aac att cac 355 Thr Ile Thr Val Ile Asp Asp Asp Thr Val Asp Ile Ser Asn Ile His 70 75 80 85 cgc caa atc ctc ttc ggc gca agc gat gtc ggt cga ccc aag gtc gag 403 Arg Gln Ile Leu Phe Gly Ala Ser Asp Val Gly Arg Pro Lys Val Glu 90 95 100 gtt gcc gcc gag cgc ctc aaa gaa ctc caa cca gac atc acc gtc aac 451 Val Ala Ala Glu Arg Leu Lys Glu Leu Gln Pro Asp Ile Thr Val Asn 105 110 115 gcg ttg cac gaa cgg atc act cca gaa aac gcc tgc gag ctg ctc aat 499 Ala Leu His Glu Arg Ile Thr Pro Glu Asn Ala Cys Glu Leu Leu Asn 120 125 130 tcc gtg gac ctc gtc tta gac ggc tcc gat tct ttc tcc aca aaa tac 547 Ser Val Asp Leu Val Leu Asp Gly Ser Asp Ser Phe Ser Thr Lys Tyr 135 140 145 tta gtg tct gat gcc gcc gaa atc acc gga act ccc ctc atc tgg gca 595 Leu Val Ser Asp Ala Ala Glu Ile Thr Gly Thr Pro Leu Ile Trp Ala 150 155 160 165 acg gta ctg cgc ttt cac ggc gaa ctg gca ctc ttc aac tct ggc ccc 643 Thr Val Leu Arg Phe His Gly Glu Leu Ala Leu Phe Asn Ser Gly Pro 170 175 180 gac cac cgc gga gtc ggc ctg cgc gac gtc ttc ccc gaa caa ccc tcc 691 Asp His Arg Gly Val Gly Leu Arg Asp Val Phe Pro Glu Gln Pro Ser 185 190 195 gcc gat ttc gtc cct gac tgc gcc acc gct ggt gtt ctt ggc gcc acc 739 Ala Asp Phe Val Pro Asp Cys Ala Thr Ala Gly Val Leu Gly Ala Thr 200 205 210 aca gcc acc atc ggc gca ctc atg gcc act cac gcc atc gga ttt ctc 787 Thr Ala Thr Ile Gly Ala Leu Met Ala Thr His Ala Ile Gly Phe Leu 215 220 225 aca gaa atc ggc gac gtc caa cca ggc aca atc ctc tcc tac gac gca 835 Thr Glu Ile Gly Asp Val Gln Pro Gly Thr Ile Leu Ser Tyr Asp Ala 230 235 240 245 ttc ccc gcc gcc acg cgc agc ttc cgc gtc tcc gcc gac ccg gcg cgc 883 Phe Pro Ala Ala Thr Arg Ser Phe Arg Val Ser Ala Asp Pro Ala Arg 250 255 260 cca ctg gtc acc cgc ctc cgc gcc tcc tac gag gca gcg cgc acc gat 931 Pro Leu Val Thr Arg Leu Arg Ala Ser Tyr Glu Ala Ala Arg Thr Asp 265 270 275 aca act tcg ctt atc gac gcc acc ctc aac ggc tcc ctc acc gcc ctc 979 Thr Thr Ser Leu Ile Asp Ala Thr Leu Asn Gly Ser Leu Thr Ala Leu 280 285 290 gat atc cga gag cca cat gaa gtt ctg ctc aaa gac ctc ccc gag ggc 1027 Asp Ile Arg Glu Pro His Glu Val Leu Leu Lys Asp Leu Pro Glu Gly 295 300 305 gca acg tca ctg aag ctc ccc tta agc cag atc acc tcg gac agc gac 1075 Ala Thr Ser Leu Lys Leu Pro Leu Ser Gln Ile Thr Ser Asp Ser Asp 310 315 320 325 att tta gag gca ctg tct gga atc gac ggc gac att ttg gtc tac tgc 1123 Ile Leu Glu Ala Leu Ser Gly Ile Asp Gly Asp Ile Leu Val Tyr Cys 330 335 340 gct tcg gga atc cgc agt tcc gac ttc atc gac aac tac tcc cac ctc 1171 Ala Ser Gly Ile Arg Ser Ser Asp Phe Ile Asp Asn Tyr Ser His Leu 345 350 355 ggc cac aaa ttt gtg aat ctt ccc ggt ggg gtc aac gcg ctg 1213 Gly His Lys Phe Val Asn Leu Pro Gly Gly Val Asn Ala Leu 360 365 370 tagctgtcaa tttaagaggc cagaattctg 1243 <210> 60 <211> 371 <212> PRT <213> Corynebacterium glutamicum <400> 60 Leu Lys Val Trp His Pro Gly Arg Ser Arg Cys Phe Asn Met Ala Gln 1 5 10 15 Leu Pro Thr Ser Glu Leu His Arg Thr Ala Arg Gln Leu Ala Leu Pro 20 25 30 Gly Tyr Gly Ile Glu Gln Gln Glu Arg Leu Phe Asn Ala His Val Leu 35 40 45 Val Ile Gly Ala Gly Gly Leu Gly Cys Pro Val Met Gln Ser Leu Ala 50 55 60 Ser Ala Gly Val Gly Thr Ile Thr Val Ile Asp Asp Asp Thr Val Asp 65 70 75 80 Ile Ser Asn Ile His Arg Gln Ile Leu Phe Gly Ala Ser Asp Val Gly 85 90 95 Arg Pro Lys Val Glu Val Ala Ala Glu Arg Leu Lys Glu Leu Gln Pro 100 105 110 Asp Ile Thr Val Asn Ala Leu His Glu Arg Ile Thr Pro Glu Asn Ala 115 120 125 Cys Glu Leu Leu Asn Ser Val Asp Leu Val Leu Asp Gly Ser Asp Ser 130 135 140 Phe Ser Thr Lys Tyr Leu Val Ser Asp Ala Ala Glu Ile Thr Gly Thr 145 150 155 160 Pro Leu Ile Trp Ala Thr Val Leu Arg Phe His Gly Glu Leu Ala Leu 165 170 175 Phe Asn Ser Gly Pro Asp His Arg Gly Val Gly Leu Arg Asp Val Phe 180 185 190 Pro Glu Gln Pro Ser Ala Asp Phe Val Pro Asp Cys Ala Thr Ala Gly 195 200 205 Val Leu Gly Ala Thr Thr Ala Thr Ile Gly Ala Leu Met Ala Thr His 210 215 220 Ala Ile Gly Phe Leu Thr Glu Ile Gly Asp Val Gln Pro Gly Thr Ile 225 230 235 240 Leu Ser Tyr Asp Ala Phe Pro Ala Ala Thr Arg Ser Phe Arg Val Ser 245 250 255 Ala Asp Pro Ala Arg Pro Leu Val Thr Arg Leu Arg Ala Ser Tyr Glu 260 265 270 Ala Ala Arg Thr Asp Thr Thr Ser Leu Ile Asp Ala Thr Leu Asn Gly 275 280 285 Ser Leu Thr Ala Leu Asp Ile Arg Glu Pro His Glu Val Leu Leu Lys 290 295 300 Asp Leu Pro Glu Gly Ala Thr Ser Leu Lys Leu Pro Leu Ser Gln Ile 305 310 315 320 Thr Ser Asp Ser Asp Ile Leu Glu Ala Leu Ser Gly Ile Asp Gly Asp 325 330 335 Ile Leu Val Tyr Cys Ala Ser Gly Ile Arg Ser Ser Asp Phe Ile Asp 340 345 350 Asn Tyr Ser His Leu Gly His Lys Phe Val Asn Leu Pro Gly Gly Val 355 360 365 Asn Ala Leu 370 <210> 61 <211> 3457 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(3427) <223> RXA01434 <400> 61 acgggatcgc ccaaaccgcc accaacccac taacgtatcg catagttatt ttcgccgtgc 60 tcgccgttaa ggcgcgcact cgtacatgaa tggtcaacaa gtg agt tct tcg ctt 115 Val Ser Ser Ser Leu 1 5 tcg aat aat tcg gag cag tcc ggc ctg cgt ggc agg atc gtt gct cca 163 Ser Asn Asn Ser Glu Gln Ser Gly Leu Arg Gly Arg Ile Val Ala Pro 10 15 20 gca ccg ccg gcg cct gtg ccc gag gcg cgc aag aag gct gtc gca cgc 211 Ala Pro Pro Ala Pro Val Pro Glu Ala Arg Lys Lys Ala Val Ala Arg 25 30 35 acg gat ggt gat cgc tcg agt ttg aaa aac tcg cct acg gca tcc gcc 259 Thr Asp Gly Asp Arg Ser Ser Leu Lys Asn Ser Pro Thr Ala Ser Ala 40 45 50 acc cag gca gcc cag acg cgc ccg gca gaa ccg gaa cca aaa aag cac 307 Thr Gln Ala Ala Gln Thr Arg Pro Ala Glu Pro Glu Pro Lys Lys His 55 60 65 acc tcc gat tct gat gtg gtg cgc tcg act ggc tcc atg gca ata gcc 355 Thr Ser Asp Ser Asp Val Val Arg Ser Thr Gly Ser Met Ala Ile Ala 70 75 80 85 acg ctg ctg agt cgt atc acc ggt ttc ctg cgc acc gtg atg att ggt 403 Thr Leu Leu Ser Arg Ile Thr Gly Phe Leu Arg Thr Val Met Ile Gly 90 95 100 gcg gcg ctg tcg ccg gcc atc gct tcg gcg ttc aac act gcc aac acg 451 Ala Ala Leu Ser Pro Ala Ile Ala Ser Ala Phe Asn Thr Ala Asn Thr 105 110 115 ctg ccc aac ctg atc act gaa atc gtg ttg ggt gcg gtg ctg aca tcg 499 Leu Pro Asn Leu Ile Thr Glu Ile Val Leu Gly Ala Val Leu Thr Ser 120 125 130 ctg gtt att ccg gtc ctt acc cgc gcg gaa aaa gaa gac gcc gac ggc 547 Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys Glu Asp Ala Asp Gly 135 140 145 ggt tcc ggg ttc ttc agg cgg ctg ctc acc ctg tcg gtg acg ctg ctg 595 Gly Ser Gly Phe Phe Arg Arg Leu Leu Thr Leu Ser Val Thr Leu Leu 150 155 160 165 ggt ggt gtc acc atc ctg tcg att atc ggc gcg ccg ctg ctg aca cgg 643 Gly Gly Val Thr Ile Leu Ser Ile Ile Gly Ala Pro Leu Leu Thr Arg 170 175 180 atg atg ctg tcc tct gag gga caa gtc aac gtg gtc atg tcc acg gcc 691 Met Met Leu Ser Ser Glu Gly Gln Val Asn Val Val Met Ser Thr Ala 185 190 195 ttt gcg tat tgg ctg ctg cca cag att ttc ttc tac ggc ctg ttt gcc 739 Phe Ala Tyr Trp Leu Leu Pro Gln Ile Phe Phe Tyr Gly Leu Phe Ala 200 205 210 ctg ttc atg gct gtg ttg aac acc cgt gaa gtg ttc aaa ccc ggc gcg 787 Leu Phe Met Ala Val Leu Asn Thr Arg Glu Val Phe Lys Pro Gly Ala 215 220 225 tgg gca cct gtt gtc aac aat gtg atc acc ttg acc gtg ctg ggc gtg 835 Trp Ala Pro Val Val Asn Asn Val Ile Thr Leu Thr Val Leu Gly Val 230 235 240 245 tac atg gtg ctg cct gcg cgt ttg cac ccg cat gag cag gtg ggc att 883 Tyr Met Val Leu Pro Ala Arg Leu His Pro His Glu Gln Val Gly Ile 250 255 260 ttt gat ccg cag atc att ttc ctc ggc gtg ggc acc acc ctt ggt gtg 931 Phe Asp Pro Gln Ile Ile Phe Leu Gly Val Gly Thr Thr Leu Gly Val 265 270 275 gtt gca cag tgt cta atc atg att ccg tac ctg cgt cgc gcg ggc att 979 Val Ala Gln Cys Leu Ile Met Ile Pro Tyr Leu Arg Arg Ala Gly Ile 280 285 290 gat atg cgc cct ctg tgg ggt atc gat gcg cgt ttg aag caa ttc ggt 1027 Asp Met Arg Pro Leu Trp Gly Ile Asp Ala Arg Leu Lys Gln Phe Gly 295 300 305 ggc atg gcg atg gcg atc atc gtg tac gtg gca atc tcc cag ttc ggt 1075 Gly Met Ala Met Ala Ile Ile Val Tyr Val Ala Ile Ser Gln Phe Gly 310 315 320 325 tac atc atc acc act cgc att gcg tcg att gca gac gat gct gcg ccg 1123 Tyr Ile Ile Thr Thr Arg Ile Ala Ser Ile Ala Asp Asp Ala Ala Pro 330 335 340 ttt att tat cag cag cac tgg atg ttg ctg caa gtt cct tat ggc atc 1171 Phe Ile Tyr Gln Gln His Trp Met Leu Leu Gln Val Pro Tyr Gly Ile 345 350 355 atc ggc gtc acc ttg ctc acc gcg att atg ccg cga ctg tcc cgc aac 1219 Ile Gly Val Thr Leu Leu Thr Ala Ile Met Pro Arg Leu Ser Arg Asn 360 365 370 gcg gca gac ggc gat gat agg gca gta gtc tct gac ctt cag ttg ggt 1267 Ala Ala Asp Gly Asp Asp Arg Ala Val Val Ser Asp Leu Gln Leu Gly 375 380 385 tcc aag cta acc ttc atc gca ctg atc ccc atc gtg gtg ttc ttc acc 1315 Ser Lys Leu Thr Phe Ile Ala Leu Ile Pro Ile Val Val Phe Phe Thr 390 395 400 405 gcc ttc ggt gtc cct att gcc aat ggc ctt ttt gcc tac ggc caa ttc 1363 Ala Phe Gly Val Pro Ile Ala Asn Gly Leu Phe Ala Tyr Gly Gln Phe 410 415 420 gat gcc aac gcc gcc aac atc ctt ggt tgg act ctg agc ttc tct gct 1411 Asp Ala Asn Ala Ala Asn Ile Leu Gly Trp Thr Leu Ser Phe Ser Ala 425 430 435 ttc acg ctg att cct tac gct ttg gtg ctg cta cat ctg cgt gtg ttt 1459 Phe Thr Leu Ile Pro Tyr Ala Leu Val Leu Leu His Leu Arg Val Phe 440 445 450 tat gcg cgt gaa gag gtc tgg acc cca acc ttc atc atc gcc ggc atc 1507 Tyr Ala Arg Glu Glu Val Trp Thr Pro Thr Phe Ile Ile Ala Gly Ile 455 460 465 acc gcc acc aag gtc gtg ctt tcc ctg ttg gca ccg ctg ctg tcg agc 1555 Thr Ala Thr Lys Val Val Leu Ser Leu Leu Ala Pro Leu Leu Ser Ser 470 475 480 485 tcc ccg gag cgt gtg gtg gtg ctt ctt ggt gcg gcc aac ggt ttc agt 1603 Ser Pro Glu Arg Val Val Val Leu Leu Gly Ala Ala Asn Gly Phe Ser 490 495 500 ttc atc acc ggc gcg gtc atc ggc gcg tat ctg ttg cgc aac aaa ctc 1651 Phe Ile Thr Gly Ala Val Ile Gly Ala Tyr Leu Leu Arg Asn Lys Leu 505 510 515 ggc ctg ttg ggt atg cgc tct ttg gct aaa acc tcc ctg tgg gcg ttg 1699 Gly Leu Leu Gly Met Arg Ser Leu Ala Lys Thr Ser Leu Trp Ala Leu 520 525 530 ggc tct gcg gcg gtt ggt gca gca gca gca tgg gcg ttg ggg tgg ctg 1747 Gly Ser Ala Ala Val Gly Ala Ala Ala Ala Trp Ala Leu Gly Trp Leu 535 540 545 att caa gcc gtc gtg ggc gat ttc ttg ctg ggc act cta agc tcc gta 1795 Ile Gln Ala Val Val Gly Asp Phe Leu Leu Gly Thr Leu Ser Ser Val 550 555 560 565 ggc tac ttg ttg aac ctg gct gtg ttg ggt gtc ttc ttc atc ttc gtc 1843 Gly Tyr Leu Leu Asn Leu Ala Val Leu Gly Val Phe Phe Ile Phe Val 570 575 580 acc ggc atc gtg ttg tca cgt tct ggt ttg ccg gag gtc caa aac ttg 1891 Thr Gly Ile Val Leu Ser Arg Ser Gly Leu Pro Glu Val Gln Asn Leu 585 590 595 ggc cag gca ctg acc cgc atc cca ggt ttg agc cgg ttt att cgc ccg 1939 Gly Gln Ala Leu Thr Arg Ile Pro Gly Leu Ser Arg Phe Ile Arg Pro 600 605 610 aat acc aag atc tct ttg gat gtc ggc gaa gtc tcc gag cag gat ttc 1987 Asn Thr Lys Ile Ser Leu Asp Val Gly Glu Val Ser Glu Gln Asp Phe 615 620 625 tcc acc cag ctg gtc gcg cca agc gag ttc gca gca acc cct gtg ccg 2035 Ser Thr Gln Leu Val Ala Pro Ser Glu Phe Ala Ala Thr Pro Val Pro 630 635 640 645 cca ccg atg tcc gcc ggt att gtc cgc gga cct cgc ctg gtt ccc ggc 2083 Pro Pro Met Ser Ala Gly Ile Val Arg Gly Pro Arg Leu Val Pro Gly 650 655 660 gcc cca gtc ggc gac ggt cgt ttc cgt ctg ctc gcc gat cac ggc ggc 2131 Ala Pro Val Gly Asp Gly Arg Phe Arg Leu Leu Ala Asp His Gly Gly 665 670 675 gtc caa ggc gcg cgt ttc tgg cag gcc cgc gag atc gcc acc ggc aag 2179 Val Gln Gly Ala Arg Phe Trp Gln Ala Arg Glu Ile Ala Thr Gly Lys 680 685 690 gaa gtc gcg ctg atc ttt gtg gat act tcc ggc aac gcc cca ttt gcg 2227 Glu Val Ala Leu Ile Phe Val Asp Thr Ser Gly Asn Ala Pro Phe Ala 695 700 705 cca ctg tct tcg gca gcc gca gcg ggc atc gcc tac gag gtg cag cgc 2275 Pro Leu Ser Ser Ala Ala Ala Ala Gly Ile Ala Tyr Glu Val Gln Arg 710 715 720 725 cgc acc aag aag ctg gcc agc ttg ggc agc ttg gcg gta gcc ccc aac 2323 Arg Thr Lys Lys Leu Ala Ser Leu Gly Ser Leu Ala Val Ala Pro Asn 730 735 740 atc cac tcc gag gcg tac cgc aac ggt tgc ctc att gtg gcc gat tgg 2371 Ile His Ser Glu Ala Tyr Arg Asn Gly Cys Leu Ile Val Ala Asp Trp 745 750 755 gtg cct ggc tcc agc ttg agc gcc gtc gcg gaa tcc ggt gcc gat ccc 2419 Val Pro Gly Ser Ser Leu Ser Ala Val Ala Glu Ser Gly Ala Asp Pro 760 765 770 cgc gcc gcc gcg ttc gcg ctc gcg gaa cta act gaa acc atc ggc gag 2467 Arg Ala Ala Ala Phe Ala Leu Ala Glu Leu Thr Glu Thr Ile Gly Glu 775 780 785 gcc cac gag atg ggt atc ccg gcc ggc ttg gac aac aag tgc cgc atc 2515 Ala His Glu Met Gly Ile Pro Ala Gly Leu Asp Asn Lys Cys Arg Ile 790 795 800 805 cgc atc aac acc gac ggc cat gcc gtc ctc gcc ttc ccg gcg att ttg 2563 Arg Ile Asn Thr Asp Gly His Ala Val Leu Ala Phe Pro Ala Ile Leu 810 815 820 ccc gat gcc tca gag ctc cgc gac gcc aag tcc ctg gcc tcg gcc gcc 2611 Pro Asp Ala Ser Glu Leu Arg Asp Ala Lys Ser Leu Ala Ser Ala Ala 825 830 835 gag atg ctt atc gac gcg acc ctc gct ccc agc gac gtc aag gca atg 2659 Glu Met Leu Ile Asp Ala Thr Leu Ala Pro Ser Asp Val Lys Ala Met 840 845 850 gtc act gaa gcc cag ggg cta gct aca gaa gac aat ccc gat tac gca 2707 Val Thr Glu Ala Gln Gly Leu Ala Thr Glu Asp Asn Pro Asp Tyr Ala 855 860 865 tca ctt gcc atg gcg atg cgc acc tgc gga ctg ttc acc gag gaa cca 2755 Ser Leu Ala Met Ala Met Arg Thr Cys Gly Leu Phe Thr Glu Glu Pro 870 875 880 885 acc cac ctt gtg gtg aag aag gaa aag aca cca aag cct gcg aca cgt 2803 Thr His Leu Val Val Lys Lys Glu Lys Thr Pro Lys Pro Ala Thr Arg 890 895 900 gat ggt ttc ggt gcc tcc gac tac acc gtc aag ggc atg gca gcc atc 2851 Asp Gly Phe Gly Ala Ser Asp Tyr Thr Val Lys Gly Met Ala Ala Ile 905 910 915 gcc gct gtg gtg atc atc ttg gtt tcc ctg gtg gcc gcc ggt acc gcg 2899 Ala Ala Val Val Ile Ile Leu Val Ser Leu Val Ala Ala Gly Thr Ala 920 925 930 ttc ctc acc agc ttc ttc ggc agc agc acc aac gaa caa tcc ccg ttg 2947 Phe Leu Thr Ser Phe Phe Gly Ser Ser Thr Asn Glu Gln Ser Pro Leu 935 940 945 gcc tct gtt gaa gcc acc acc tct gca aca cca gaa cct gtg ggg cca 2995 Ala Ser Val Glu Ala Thr Thr Ser Ala Thr Pro Glu Pro Val Gly Pro 950 955 960 965 ccg gtc tac ctg gat ctg gat caa gcc cgc acg tgg gat gac ggt gca 3043 Pro Val Tyr Leu Asp Leu Asp Gln Ala Arg Thr Trp Asp Asp Gly Ala 970 975 980 gga aca gat gtc acc gac gtc acc gac ggc aac acc tcc acc gca tgg 3091 Gly Thr Asp Val Thr Asp Val Thr Asp Gly Asn Thr Ser Thr Ala Trp 985 990 995 acc tcc acc ggc ggc gac ggc ctc cta gtt gac ctg tcc acg cct gcc 3139 Thr Ser Thr Gly Gly Asp Gly Leu Leu Val Asp Leu Ser Thr Pro Ala 1000 1005 1010 cgc ctc gac cgc gtc atc ttg acc acc ggc acc ggc tcc gac agc aac 3187 Arg Leu Asp Arg Val Ile Leu Thr Thr Gly Thr Gly Ser Asp Ser Asn 1015 1020 1025 gtg acc tcg acc gtg aag atc tac gca ttc aac gac gcc tca cca cac 3235 Val Thr Ser Thr Val Lys Ile Tyr Ala Phe Asn Asp Ala Ser Pro His 1030 1035 1040 1045 tcc ctg tcg gaa ggc atc gag atc ggc acc gtg gat tat tcc ggc cgc 3283 Ser Leu Ser Glu Gly Ile Glu Ile Gly Thr Val Asp Tyr Ser Gly Arg 1050 1055 1060 agt ctc agc cac agc atc cgc gat tcc tcc aag ctt ccg ggt cag gtg 3331 Ser Leu Ser His Ser Ile Arg Asp Ser Ser Lys Leu Pro Gly Gln Val 1065 1070 1075 gaa tcc gtg gtg att ctg gtc gat gag gtt cgt tcc tca caa acc tca 3379 Glu Ser Val Val Ile Leu Val Asp Glu Val Arg Ser Ser Gln Thr Ser 1080 1085 1090 gac acc aat cca cag atg cag atc gct gaa gta caa ctt gtt ggt tgg 3427 Asp Thr Asn Pro Gln Met Gln Ile Ala Glu Val Gln Leu Val Gly Trp 1095 1100 1105 taaattacgc gtttgtgatt gacccccatt 3457 <210> 62 <211> 1109 <212> PRT <213> Corynebacterium glutamicum <400> 62 Val Ser Ser Ser Leu Ser Asn Asn Ser Glu Gln Ser Gly Leu Arg Gly 1 5 10 15 Arg Ile Val Ala Pro Ala Pro Pro Ala Pro Val Pro Glu Ala Arg Lys 20 25 30 Lys Ala Val Ala Arg Thr Asp Gly Asp Arg Ser Ser Leu Lys Asn Ser 35 40 45 Pro Thr Ala Ser Ala Thr Gln Ala Ala Gln Thr Arg Pro Ala Glu Pro 50 55 60 Glu Pro Lys Lys His Thr Ser Asp Ser Asp Val Val Arg Ser Thr Gly 65 70 75 80 Ser Met Ala Ile Ala Thr Leu Leu Ser Arg Ile Thr Gly Phe Leu Arg 85 90 95 Thr Val Met Ile Gly Ala Ala Leu Ser Pro Ala Ile Ala Ser Ala Phe 100 105 110 Asn Thr Ala Asn Thr Leu Pro Asn Leu Ile Thr Glu Ile Val Leu Gly 115 120 125 Ala Val Leu Thr Ser Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys 130 135 140 Glu Asp Ala Asp Gly Gly Ser Gly Phe Phe Arg Arg Leu Leu Thr Leu 145 150 155 160 Ser Val Thr Leu Leu Gly Gly Val Thr Ile Leu Ser Ile Ile Gly Ala 165 170 175 Pro Leu Leu Thr Arg Met Met Leu Ser Ser Glu Gly Gln Val Asn Val 180 185 190 Val Met Ser Thr Ala Phe Ala Tyr Trp Leu Leu Pro Gln Ile Phe Phe 195 200 205 Tyr Gly Leu Phe Ala Leu Phe Met Ala Val Leu Asn Thr Arg Glu Val 210 215 220 Phe Lys Pro Gly Ala Trp Ala Pro Val Val Asn Asn Val Ile Thr Leu 225 230 235 240 Thr Val Leu Gly Val Tyr Met Val Leu Pro Ala Arg Leu His Pro His 245 250 255 Glu Gln Val Gly Ile Phe Asp Pro Gln Ile Ile Phe Leu Gly Val Gly 260 265 270 Thr Thr Leu Gly Val Val Ala Gln Cys Leu Ile Met Ile Pro Tyr Leu 275 280 285 Arg Arg Ala Gly Ile Asp Met Arg Pro Leu Trp Gly Ile Asp Ala Arg 290 295 300 Leu Lys Gln Phe Gly Gly Met Ala Met Ala Ile Ile Val Tyr Val Ala 305 310 315 320 Ile Ser Gln Phe Gly Tyr Ile Ile Thr Thr Arg Ile Ala Ser Ile Ala 325 330 335 Asp Asp Ala Ala Pro Phe Ile Tyr Gln Gln His Trp Met Leu Leu Gln 340 345 350 Val Pro Tyr Gly Ile Ile Gly Val Thr Leu Leu Thr Ala Ile Met Pro 355 360 365 Arg Leu Ser Arg Asn Ala Ala Asp Gly Asp Asp Arg Ala Val Val Ser 370 375 380 Asp Leu Gln Leu Gly Ser Lys Leu Thr Phe Ile Ala Leu Ile Pro Ile 385 390 395 400 Val Val Phe Phe Thr Ala Phe Gly Val Pro Ile Ala Asn Gly Leu Phe 405 410 415 Ala Tyr Gly Gln Phe Asp Ala Asn Ala Ala Asn Ile Leu Gly Trp Thr 420 425 430 Leu Ser Phe Ser Ala Phe Thr Leu Ile Pro Tyr Ala Leu Val Leu Leu 435 440 445 His Leu Arg Val Phe Tyr Ala Arg Glu Glu Val Trp Thr Pro Thr Phe 450 455 460 Ile Ile Ala Gly Ile Thr Ala Thr Lys Val Val Leu Ser Leu Leu Ala 465 470 475 480 Pro Leu Leu Ser Ser Ser Pro Glu Arg Val Val Val Leu Leu Gly Ala 485 490 495 Ala Asn Gly Phe Ser Phe Ile Thr Gly Ala Val Ile Gly Ala Tyr Leu 500 505 510 Leu Arg Asn Lys Leu Gly Leu Leu Gly Met Arg Ser Leu Ala Lys Thr 515 520 525 Ser Leu Trp Ala Leu Gly Ser Ala Ala Val Gly Ala Ala Ala Ala Trp 530 535 540 Ala Leu Gly Trp Leu Ile Gln Ala Val Val Gly Asp Phe Leu Leu Gly 545 550 555 560 Thr Leu Ser Ser Val Gly Tyr Leu Leu Asn Leu Ala Val Leu Gly Val 565 570 575 Phe Phe Ile Phe Val Thr Gly Ile Val Leu Ser Arg Ser Gly Leu Pro 580 585 590 Glu Val Gln Asn Leu Gly Gln Ala Leu Thr Arg Ile Pro Gly Leu Ser 595 600 605 Arg Phe Ile Arg Pro Asn Thr Lys Ile Ser Leu Asp Val Gly Glu Val 610 615 620 Ser Glu Gln Asp Phe Ser Thr Gln Leu Val Ala Pro Ser Glu Phe Ala 625 630 635 640 Ala Thr Pro Val Pro Pro Pro Met Ser Ala Gly Ile Val Arg Gly Pro 645 650 655 Arg Leu Val Pro Gly Ala Pro Val Gly Asp Gly Arg Phe Arg Leu Leu 660 665 670 Ala Asp His Gly Gly Val Gln Gly Ala Arg Phe Trp Gln Ala Arg Glu 675 680 685 Ile Ala Thr Gly Lys Glu Val Ala Leu Ile Phe Val Asp Thr Ser Gly 690 695 700 Asn Ala Pro Phe Ala Pro Leu Ser Ser Ala Ala Ala Ala Gly Ile Ala 705 710 715 720 Tyr Glu Val Gln Arg Arg Thr Lys Lys Leu Ala Ser Leu Gly Ser Leu 725 730 735 Ala Val Ala Pro Asn Ile His Ser Glu Ala Tyr Arg Asn Gly Cys Leu 740 745 750 Ile Val Ala Asp Trp Val Pro Gly Ser Ser Leu Ser Ala Val Ala Glu 755 760 765 Ser Gly Ala Asp Pro Arg Ala Ala Ala Phe Ala Leu Ala Glu Leu Thr 770 775 780 Glu Thr Ile Gly Glu Ala His Glu Met Gly Ile Pro Ala Gly Leu Asp 785 790 795 800 Asn Lys Cys Arg Ile Arg Ile Asn Thr Asp Gly His Ala Val Leu Ala 805 810 815 Phe Pro Ala Ile Leu Pro Asp Ala Ser Glu Leu Arg Asp Ala Lys Ser 820 825 830 Leu Ala Ser Ala Ala Glu Met Leu Ile Asp Ala Thr Leu Ala Pro Ser 835 840 845 Asp Val Lys Ala Met Val Thr Glu Ala Gln Gly Leu Ala Thr Glu Asp 850 855 860 Asn Pro Asp Tyr Ala Ser Leu Ala Met Ala Met Arg Thr Cys Gly Leu 865 870 875 880 Phe Thr Glu Glu Pro Thr His Leu Val Val Lys Lys Glu Lys Thr Pro 885 890 895 Lys Pro Ala Thr Arg Asp Gly Phe Gly Ala Ser Asp Tyr Thr Val Lys 900 905 910 Gly Met Ala Ala Ile Ala Ala Val Val Ile Ile Leu Val Ser Leu Val 915 920 925 Ala Ala Gly Thr Ala Phe Leu Thr Ser Phe Phe Gly Ser Ser Thr Asn 930 935 940 Glu Gln Ser Pro Leu Ala Ser Val Glu Ala Thr Thr Ser Ala Thr Pro 945 950 955 960 Glu Pro Val Gly Pro Pro Val Tyr Leu Asp Leu Asp Gln Ala Arg Thr 965 970 975 Trp Asp Asp Gly Ala Gly Thr Asp Val Thr Asp Val Thr Asp Gly Asn 980 985 990 Thr Ser Thr Ala Trp Thr Ser Thr Gly Gly Asp Gly Leu Leu Val Asp 995 1000 1005 Leu Ser Thr Pro Ala Arg Leu Asp Arg Val Ile Leu Thr Thr Gly Thr 1010 1015 1020 Gly Ser Asp Ser Asn Val Thr Ser Thr Val Lys Ile Tyr Ala Phe Asn 1025 1030 1035 1040 Asp Ala Ser Pro His Ser Leu Ser Glu Gly Ile Glu Ile Gly Thr Val 1045 1050 1055 Asp Tyr Ser Gly Arg Ser Leu Ser His Ser Ile Arg Asp Ser Ser Lys 1060 1065 1070 Leu Pro Gly Gln Val Glu Ser Val Val Ile Leu Val Asp Glu Val Arg 1075 1080 1085 Ser Ser Gln Thr Ser Asp Thr Asn Pro Gln Met Gln Ile Ala Glu Val 1090 1095 1100 Gln Leu Val Gly Trp 1105 <210> 63 <211> 1354 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1324) <223> RXA01442 <400> 63 aattaagcaa cctttttaac aggtttagag ctttcacgag gtggcgtcag ataaagtgaa 60 caccaatatt tctgacatct tccaacggag gctaaaaggc atg tac atc cca gag 115 Met Tyr Ile Pro Glu 1 5 tcg atc ggc acc cct ttg acc ccc aat gcc acg aaa gtg atg ctg ctg 163 Ser Ile Gly Thr Pro Leu Thr Pro Asn Ala Thr Lys Val Met Leu Leu 10 15 20 gga tca gga gaa tta ggc aaa gaa gta gcc atc gct ttc cag cgt ctc 211 Gly Ser Gly Glu Leu Gly Lys Glu Val Ala Ile Ala Phe Gln Arg Leu 25 30 35 ggc ctg gaa gtc cat gca gtt gat cgc tac gaa cat gcc cca gcc cac 259 Gly Leu Glu Val His Ala Val Asp Arg Tyr Glu His Ala Pro Ala His 40 45 50 cag gtc gct cac ttc tcc tat gtc atc gac atg aca gat gca gcc cag 307 Gln Val Ala His Phe Ser Tyr Val Ile Asp Met Thr Asp Ala Ala Gln 55 60 65 gtg cgg gaa ttg gtg gag cgt gtg cgc cca gat ttt gtc att cct gaa 355 Val Arg Glu Leu Val Glu Arg Val Arg Pro Asp Phe Val Ile Pro Glu 70 75 80 85 atc gaa gca ctg gca acc gat gaa ctg gtg aag atc gaa gaa gag ggg 403 Ile Glu Ala Leu Ala Thr Asp Glu Leu Val Lys Ile Glu Glu Glu Gly 90 95 100 cta gct acc atc gtg ccc act gca cgt gca gcc aag ctg acc atg aac 451 Leu Ala Thr Ile Val Pro Thr Ala Arg Ala Ala Lys Leu Thr Met Asn 105 110 115 cgc gaa ggc atc cgc aag ctg gcg gca gag gaa ctg ggt ctt cca acc 499 Arg Glu Gly Ile Arg Lys Leu Ala Ala Glu Glu Leu Gly Leu Pro Thr 120 125 130 tcc aac tat gag ttc tgc tcc act ttc gag gaa ttc tcc gca gct gct 547 Ser Asn Tyr Glu Phe Cys Ser Thr Phe Glu Glu Phe Ser Ala Ala Ala 135 140 145 gaa aag ctt ggt tac ccc aac gtg gtg aaa cca gtg atg agt tct tcc 595 Glu Lys Leu Gly Tyr Pro Asn Val Val Lys Pro Val Met Ser Ser Ser 150 155 160 165 ggc aag ggc caa tct gtt ttg cgt agt tca gac gat ctg cag gca gca 643 Gly Lys Gly Gln Ser Val Leu Arg Ser Ser Asp Asp Leu Gln Ala Ala 170 175 180 tgg gat tat gcg atg agc ggt gca cgc gtg gcc aac tcc cgc gtc atc 691 Trp Asp Tyr Ala Met Ser Gly Ala Arg Val Ala Asn Ser Arg Val Ile 185 190 195 gtg gaa gct ttc gtg gaa ttc gat tac gag atc acc ctg ttg aca gta 739 Val Glu Ala Phe Val Glu Phe Asp Tyr Glu Ile Thr Leu Leu Thr Val 200 205 210 agg tcc atc gat ccc acc acc tct aag cct gcg acc tgg ttc tgt gag 787 Arg Ser Ile Asp Pro Thr Thr Ser Lys Pro Ala Thr Trp Phe Cys Glu 215 220 225 ccc att ggg cac cgc caa gaa gac ggc gac tac gtg gaa tcc tgg cag 835 Pro Ile Gly His Arg Gln Glu Asp Gly Asp Tyr Val Glu Ser Trp Gln 230 235 240 245 cca atg gag atg act cct cgc gcg ctg gaa aac gca cgc tca gta gcc 883 Pro Met Glu Met Thr Pro Arg Ala Leu Glu Asn Ala Arg Ser Val Ala 250 255 260 gca cgc atc acc aac gca ttg ggc gga cgc ggc gta ttt ggt gtg gag 931 Ala Arg Ile Thr Asn Ala Leu Gly Gly Arg Gly Val Phe Gly Val Glu 265 270 275 ctc ttt gtc tcc ggc gat gac gtg tac ttc tct gaa gtc tcc cca cgc 979 Leu Phe Val Ser Gly Asp Asp Val Tyr Phe Ser Glu Val Ser Pro Arg 280 285 290 cca cac gac acc ggc ctt gtc acc ctt gcc acc cag cgt ttc tct gaa 1027 Pro His Asp Thr Gly Leu Val Thr Leu Ala Thr Gln Arg Phe Ser Glu 295 300 305 ttt gaa ctc cac gcc aag gca att ctg gga ttg cct gtt gat gtc acc 1075 Phe Glu Leu His Ala Lys Ala Ile Leu Gly Leu Pro Val Asp Val Thr 310 315 320 325 ctg att tct cca ggt gcc tcc gct gtc atc tac ggt ggc atc gaa tct 1123 Leu Ile Ser Pro Gly Ala Ser Ala Val Ile Tyr Gly Gly Ile Glu Ser 330 335 340 gaa ggc gtg agc tac acc ggg ttg gct gaa gcg ctg gca gtg gct gaa 1171 Glu Gly Val Ser Tyr Thr Gly Leu Ala Glu Ala Leu Ala Val Ala Glu 345 350 355 act gat ctt cgt atc ttt gcc aag cca gag gcc ttc acc aag cgt cgc 1219 Thr Asp Leu Arg Ile Phe Ala Lys Pro Glu Ala Phe Thr Lys Arg Arg 360 365 370 atg ggt gtt gca gtg tcc acc gct gag gat gtg gct gca gcc agg gac 1267 Met Gly Val Ala Val Ser Thr Ala Glu Asp Val Ala Ala Ala Arg Asp 375 380 385 cgc gcc act ttg gct gcc gcg gcg atc aag gtt cat cca gga aat tcc 1315 Arg Ala Thr Leu Ala Ala Ala Ala Ile Lys Val His Pro Gly Asn Ser 390 395 400 405 gca gag gct taacatgctg ggaaagcatc ggggcagcat 1354 Ala Glu Ala <210> 64 <211> 408 <212> PRT <213> Corynebacterium glutamicum <400> 64 Met Tyr Ile Pro Glu Ser Ile Gly Thr Pro Leu Thr Pro Asn Ala Thr 1 5 10 15 Lys Val Met Leu Leu Gly Ser Gly Glu Leu Gly Lys Glu Val Ala Ile 20 25 30 Ala Phe Gln Arg Leu Gly Leu Glu Val His Ala Val Asp Arg Tyr Glu 35 40 45 His Ala Pro Ala His Gln Val Ala His Phe Ser Tyr Val Ile Asp Met 50 55 60 Thr Asp Ala Ala Gln Val Arg Glu Leu Val Glu Arg Val Arg Pro Asp 65 70 75 80 Phe Val Ile Pro Glu Ile Glu Ala Leu Ala Thr Asp Glu Leu Val Lys 85 90 95 Ile Glu Glu Glu Gly Leu Ala Thr Ile Val Pro Thr Ala Arg Ala Ala 100 105 110 Lys Leu Thr Met Asn Arg Glu Gly Ile Arg Lys Leu Ala Ala Glu Glu 115 120 125 Leu Gly Leu Pro Thr Ser Asn Tyr Glu Phe Cys Ser Thr Phe Glu Glu 130 135 140 Phe Ser Ala Ala Ala Glu Lys Leu Gly Tyr Pro Asn Val Val Lys Pro 145 150 155 160 Val Met Ser Ser Ser Gly Lys Gly Gln Ser Val Leu Arg Ser Ser Asp 165 170 175 Asp Leu Gln Ala Ala Trp Asp Tyr Ala Met Ser Gly Ala Arg Val Ala 180 185 190 Asn Ser Arg Val Ile Val Glu Ala Phe Val Glu Phe Asp Tyr Glu Ile 195 200 205 Thr Leu Leu Thr Val Arg Ser Ile Asp Pro Thr Thr Ser Lys Pro Ala 210 215 220 Thr Trp Phe Cys Glu Pro Ile Gly His Arg Gln Glu Asp Gly Asp Tyr 225 230 235 240 Val Glu Ser Trp Gln Pro Met Glu Met Thr Pro Arg Ala Leu Glu Asn 245 250 255 Ala Arg Ser Val Ala Ala Arg Ile Thr Asn Ala Leu Gly Gly Arg Gly 260 265 270 Val Phe Gly Val Glu Leu Phe Val Ser Gly Asp Asp Val Tyr Phe Ser 275 280 285 Glu Val Ser Pro Arg Pro His Asp Thr Gly Leu Val Thr Leu Ala Thr 290 295 300 Gln Arg Phe Ser Glu Phe Glu Leu His Ala Lys Ala Ile Leu Gly Leu 305 310 315 320 Pro Val Asp Val Thr Leu Ile Ser Pro Gly Ala Ser Ala Val Ile Tyr 325 330 335 Gly Gly Ile Glu Ser Glu Gly Val Ser Tyr Thr Gly Leu Ala Glu Ala 340 345 350 Leu Ala Val Ala Glu Thr Asp Leu Arg Ile Phe Ala Lys Pro Glu Ala 355 360 365 Phe Thr Lys Arg Arg Met Gly Val Ala Val Ser Thr Ala Glu Asp Val 370 375 380 Ala Ala Ala Arg Asp Arg Ala Thr Leu Ala Ala Ala Ala Ile Lys Val 385 390 395 400 His Pro Gly Asn Ser Ala Glu Ala 405 <210> 65 <211> 1402 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1372) <223> RXA01483 <400> 65 ggtggccgat aaggtggaag catctttgcg cgcaatccaa ggtctgctgc gcccttcgcg 60 gcgtgcacgc catgagcaac ttcggaggct gaaaaagtag atg tac ccc tat tcc 115 Met Tyr Pro Tyr Ser 1 5 gac gca gac gct ttt cga cgc cag cct gag cgc gcc aag tcc agc caa 163 Asp Ala Asp Ala Phe Arg Arg Gln Pro Glu Arg Ala Lys Ser Ser Gln 10 15 20 ctg cgt acc agc gcc gta gac acc cgc agc gcg ttc gcc cgc gac cgg 211 Leu Arg Thr Ser Ala Val Asp Thr Arg Ser Ala Phe Ala Arg Asp Arg 25 30 35 gct cgc gtg ctg cat tct gct gct ctt cga cgc ctc gcg gat aaa acc 259 Ala Arg Val Leu His Ser Ala Ala Leu Arg Arg Leu Ala Asp Lys Thr 40 45 50 caa gtg gtt ggc ccc aat gat ggt gat act ccg cgc acc cgg ctg acg 307 Gln Val Val Gly Pro Asn Asp Gly Asp Thr Pro Arg Thr Arg Leu Thr 55 60 65 cac tct ttg gaa gta gct caa att gca cgg gga atc gga gct gga ctg 355 His Ser Leu Glu Val Ala Gln Ile Ala Arg Gly Ile Gly Ala Gly Leu 70 75 80 85 gat ttg gat cct gat ctg tgc gat ctg gca ggg ctg tgc cat gac att 403 Asp Leu Asp Pro Asp Leu Cys Asp Leu Ala Gly Leu Cys His Asp Ile 90 95 100 ggg cat ccg ccg tat gga cac aac ggt gaa aac gcg ttg aat gaa gtt 451 Gly His Pro Pro Tyr Gly His Asn Gly Glu Asn Ala Leu Asn Glu Val 105 110 115 gct gcg gcc tgt gga gga ttt gag ggc aac gcc caa acc ttg cgt att 499 Ala Ala Ala Cys Gly Gly Phe Glu Gly Asn Ala Gln Thr Leu Arg Ile 120 125 130 ctc acg cgt ctg gag cca aaa att gtc tct gat gag ggg gag agc ttt 547 Leu Thr Arg Leu Glu Pro Lys Ile Val Ser Asp Glu Gly Glu Ser Phe 135 140 145 ggg ctg aac ttg tcg cgg gct gct ctt gat gct gca tgt aag tat ccg 595 Gly Leu Asn Leu Ser Arg Ala Ala Leu Asp Ala Ala Cys Lys Tyr Pro 150 155 160 165 tgg gct aaa aca aat gcg gat ggc agt gtc aat aag aaa tac agt gct 643 Trp Ala Lys Thr Asn Ala Asp Gly Ser Val Asn Lys Lys Tyr Ser Ala 170 175 180 tat gac gag gac gca gaa atc ctc gct tgg att aga caa ggc cat gag 691 Tyr Asp Glu Asp Ala Glu Ile Leu Ala Trp Ile Arg Gln Gly His Glu 185 190 195 gat ctt cgc cca ccg atc gaa gcg cag gtc atg gac ttt tct gat gac 739 Asp Leu Arg Pro Pro Ile Glu Ala Gln Val Met Asp Phe Ser Asp Asp 200 205 210 att gcc tac tcg gtg cac gat gtg gaa gac ggt att gta tcc ggg cgt 787 Ile Ala Tyr Ser Val His Asp Val Glu Asp Gly Ile Val Ser Gly Arg 215 220 225 atc gac tta aaa gtg ctg tgg gac ctg gtt gaa tta gca gct ttg gca 835 Ile Asp Leu Lys Val Leu Trp Asp Leu Val Glu Leu Ala Ala Leu Ala 230 235 240 245 gac aaa gga gca gct gct ttc gga ggt tcg cct gca gag ctc atc gag 883 Asp Lys Gly Ala Ala Ala Phe Gly Gly Ser Pro Ala Glu Leu Ile Glu 250 255 260 ggt gca gca tcg ttg cgg gaa ctt ccg gtg gtg gct gcc gct gcg gat 931 Gly Ala Ala Ser Leu Arg Glu Leu Pro Val Val Ala Ala Ala Ala Asp 265 270 275 ttt gat ttc tca ctg cgt tcc tac gct gcg ctg aag gcg atg act tca 979 Phe Asp Phe Ser Leu Arg Ser Tyr Ala Ala Leu Lys Ala Met Thr Ser 280 285 290 gaa cta gtg gga aga tac gtt ggc tct acc atc gag tca aca aag aaa 1027 Glu Leu Val Gly Arg Tyr Val Gly Ser Thr Ile Glu Ser Thr Lys Lys 295 300 305 aca cac gct ggc att gat gtg gga cgc atg cac ggc gat ttg atc att 1075 Thr His Ala Gly Ile Asp Val Gly Arg Met His Gly Asp Leu Ile Ile 310 315 320 325 cca gaa aca gcg gcc agt gaa gta aaa ctg ctc aaa acg tta gcg gtt 1123 Pro Glu Thr Ala Ala Ser Glu Val Lys Leu Leu Lys Thr Leu Ala Val 330 335 340 ctc tac gtg atg gat gac cca ggg cac ctt gcg cgc caa aac agg caa 1171 Leu Tyr Val Met Asp Asp Pro Gly His Leu Ala Arg Gln Asn Arg Gln 345 350 355 cgg gat cgt atc ttc cgg gtt ttt gac tac ctg gtg ctg ggg gct ccg 1219 Arg Asp Arg Ile Phe Arg Val Phe Asp Tyr Leu Val Leu Gly Ala Pro 360 365 370 gga tcg ttg gat ccg atg tat cgc cag tgg ttt att gaa gcg gat tca 1267 Gly Ser Leu Asp Pro Met Tyr Arg Gln Trp Phe Ile Glu Ala Asp Ser 375 380 385 gaa tcg gaa cag atc cgt gtg att gtt gat cag att gcg tcg atg acg 1315 Glu Ser Glu Gln Ile Arg Val Ile Val Asp Gln Ile Ala Ser Met Thr 390 395 400 405 gag tct cgt ctg gaa cgc ctt gcc cgg aat gct gct gac atc tca gga 1363 Glu Ser Arg Leu Glu Arg Leu Ala Arg Asn Ala Ala Asp Ile Ser Gly 410 415 420 ttt ttg gga taattggtta gagcagcagt aagtttcagt 1402 Phe Leu Gly <210> 66 <211> 424 <212> PRT <213> Corynebacterium glutamicum <400> 66 Met Tyr Pro Tyr Ser Asp Ala Asp Ala Phe Arg Arg Gln Pro Glu Arg 1 5 10 15 Ala Lys Ser Ser Gln Leu Arg Thr Ser Ala Val Asp Thr Arg Ser Ala 20 25 30 Phe Ala Arg Asp Arg Ala Arg Val Leu His Ser Ala Ala Leu Arg Arg 35 40 45 Leu Ala Asp Lys Thr Gln Val Val Gly Pro Asn Asp Gly Asp Thr Pro 50 55 60 Arg Thr Arg Leu Thr His Ser Leu Glu Val Ala Gln Ile Ala Arg Gly 65 70 75 80 Ile Gly Ala Gly Leu Asp Leu Asp Pro Asp Leu Cys Asp Leu Ala Gly 85 90 95 Leu Cys His Asp Ile Gly His Pro Pro Tyr Gly His Asn Gly Glu Asn 100 105 110 Ala Leu Asn Glu Val Ala Ala Ala Cys Gly Gly Phe Glu Gly Asn Ala 115 120 125 Gln Thr Leu Arg Ile Leu Thr Arg Leu Glu Pro Lys Ile Val Ser Asp 130 135 140 Glu Gly Glu Ser Phe Gly Leu Asn Leu Ser Arg Ala Ala Leu Asp Ala 145 150 155 160 Ala Cys Lys Tyr Pro Trp Ala Lys Thr Asn Ala Asp Gly Ser Val Asn 165 170 175 Lys Lys Tyr Ser Ala Tyr Asp Glu Asp Ala Glu Ile Leu Ala Trp Ile 180 185 190 Arg Gln Gly His Glu Asp Leu Arg Pro Pro Ile Glu Ala Gln Val Met 195 200 205 Asp Phe Ser Asp Asp Ile Ala Tyr Ser Val His Asp Val Glu Asp Gly 210 215 220 Ile Val Ser Gly Arg Ile Asp Leu Lys Val Leu Trp Asp Leu Val Glu 225 230 235 240 Leu Ala Ala Leu Ala Asp Lys Gly Ala Ala Ala Phe Gly Gly Ser Pro 245 250 255 Ala Glu Leu Ile Glu Gly Ala Ala Ser Leu Arg Glu Leu Pro Val Val 260 265 270 Ala Ala Ala Ala Asp Phe Asp Phe Ser Leu Arg Ser Tyr Ala Ala Leu 275 280 285 Lys Ala Met Thr Ser Glu Leu Val Gly Arg Tyr Val Gly Ser Thr Ile 290 295 300 Glu Ser Thr Lys Lys Thr His Ala Gly Ile Asp Val Gly Arg Met His 305 310 315 320 Gly Asp Leu Ile Ile Pro Glu Thr Ala Ala Ser Glu Val Lys Leu Leu 325 330 335 Lys Thr Leu Ala Val Leu Tyr Val Met Asp Asp Pro Gly His Leu Ala 340 345 350 Arg Gln Asn Arg Gln Arg Asp Arg Ile Phe Arg Val Phe Asp Tyr Leu 355 360 365 Val Leu Gly Ala Pro Gly Ser Leu Asp Pro Met Tyr Arg Gln Trp Phe 370 375 380 Ile Glu Ala Asp Ser Glu Ser Glu Gln Ile Arg Val Ile Val Asp Gln 385 390 395 400 Ile Ala Ser Met Thr Glu Ser Arg Leu Glu Arg Leu Ala Arg Asn Ala 405 410 415 Ala Asp Ile Ser Gly Phe Leu Gly 420 <210> 67 <211> 982 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(952) <223> RXA01515 <400> 67 taagcctggt gctgtgacca cgacgtctgc ggtgcgcggc ggttttaaga acaacgctgc 60 ctcccgcgct gaggtgttct ccctgattcg ggggcactaa atg aac gta tcc tct 115 Met Asn Val Ser Ser 1 5 ttg acc atc ccg gga cgc tgt ttg gtc atg gga att gtc aat gtc act 163 Leu Thr Ile Pro Gly Arg Cys Leu Val Met Gly Ile Val Asn Val Thr 10 15 20 gag gat tcc ttt tcg gac ggt ggc aag tac att gac gtt gat cag gcg 211 Glu Asp Ser Phe Ser Asp Gly Gly Lys Tyr Ile Asp Val Asp Gln Ala 25 30 35 atc gcg cat gcc aag gaa ttg gtg gct gct ggc gcc gac atg att gat 259 Ile Ala His Ala Lys Glu Leu Val Ala Ala Gly Ala Asp Met Ile Asp 40 45 50 gtc ggc ggc gag tcc acc cgg cct ggg gca gtg cgc gtc gac gcg tcc 307 Val Gly Gly Glu Ser Thr Arg Pro Gly Ala Val Arg Val Asp Ala Ser 55 60 65 gtg gaa cgg gac cgg gtt gtg ccg gtc att aag gcg ctt cac gac gcc 355 Val Glu Arg Asp Arg Val Val Pro Val Ile Lys Ala Leu His Asp Ala 70 75 80 85 ggc atc cac act tcc gta gac acc atg cgg gcc tcc gtg gcg cag gct 403 Gly Ile His Thr Ser Val Asp Thr Met Arg Ala Ser Val Ala Gln Ala 90 95 100 gcc gcg ggc gct ggc gtc tcc atg atc aac gac gtc tct ggc ggt ttg 451 Ala Ala Gly Ala Gly Val Ser Met Ile Asn Asp Val Ser Gly Gly Leu 105 110 115 gct gat cct gag atg ttt tct gtc atg gcg gaa gcg caa att ccc gtg 499 Ala Asp Pro Glu Met Phe Ser Val Met Ala Glu Ala Gln Ile Pro Val 120 125 130 tgt ttg atg cac tgg cgc acc ctc caa ttc ggt gat gcc gca ggt cag 547 Cys Leu Met His Trp Arg Thr Leu Gln Phe Gly Asp Ala Ala Gly Gln 135 140 145 gca gat cac ggt gga gac gtt gta gcc gat gtg cac gca gtg ctt gat 595 Ala Asp His Gly Gly Asp Val Val Ala Asp Val His Ala Val Leu Asp 150 155 160 165 gat ctt gtc gcc cgc gcc acc gct gct ggt gtg gcc gaa aac cag atc 643 Asp Leu Val Ala Arg Ala Thr Ala Ala Gly Val Ala Glu Asn Gln Ile 170 175 180 gtg ctt gat cca ggt ttg ggt ttt gcc aaa tca cgt gaa gac aac tgg 691 Val Leu Asp Pro Gly Leu Gly Phe Ala Lys Ser Arg Glu Asp Asn Trp 185 190 195 cgt ttg ctg caa gca ctg ccc gag ttt att tct gga cct ttc ccc atc 739 Arg Leu Leu Gln Ala Leu Pro Glu Phe Ile Ser Gly Pro Phe Pro Ile 200 205 210 ctg gtg gga gca tcc cgg aag cga ttc ctg gct ggc gtg cgc aaa gac 787 Leu Val Gly Ala Ser Arg Lys Arg Phe Leu Ala Gly Val Arg Lys Asp 215 220 225 cgt ggc cta gat gtc acc ccc att gat gcc gac cca gca acc gca gcg 835 Arg Gly Leu Asp Val Thr Pro Ile Asp Ala Asp Pro Ala Thr Ala Ala 230 235 240 245 gtg acc gca gtg tct gca cat atg gga gca tgg ggt gtg cgc gtg cac 883 Val Thr Ala Val Ser Ala His Met Gly Ala Trp Gly Val Arg Val His 250 255 260 gat gtc cca gta tca agg gac gct gtt gat gtt gcc gca ttg tgg cga 931 Asp Val Pro Val Ser Arg Asp Ala Val Asp Val Ala Ala Leu Trp Arg 265 270 275 agt gga gga act cac cat ggc tgatcgtatt gaacttaaag gccttgaatg 982 Ser Gly Gly Thr His His Gly 280 <210> 68 <211> 284 <212> PRT <213> Corynebacterium glutamicum <400> 68 Met Asn Val Ser Ser Leu Thr Ile Pro Gly Arg Cys Leu Val Met Gly 1 5 10 15 Ile Val Asn Val Thr Glu Asp Ser Phe Ser Asp Gly Gly Lys Tyr Ile 20 25 30 Asp Val Asp Gln Ala Ile Ala His Ala Lys Glu Leu Val Ala Ala Gly 35 40 45 Ala Asp Met Ile Asp Val Gly Gly Glu Ser Thr Arg Pro Gly Ala Val 50 55 60 Arg Val Asp Ala Ser Val Glu Arg Asp Arg Val Val Pro Val Ile Lys 65 70 75 80 Ala Leu His Asp Ala Gly Ile His Thr Ser Val Asp Thr Met Arg Ala 85 90 95 Ser Val Ala Gln Ala Ala Ala Gly Ala Gly Val Ser Met Ile Asn Asp 100 105 110 Val Ser Gly Gly Leu Ala Asp Pro Glu Met Phe Ser Val Met Ala Glu 115 120 125 Ala Gln Ile Pro Val Cys Leu Met His Trp Arg Thr Leu Gln Phe Gly 130 135 140 Asp Ala Ala Gly Gln Ala Asp His Gly Gly Asp Val Val Ala Asp Val 145 150 155 160 His Ala Val Leu Asp Asp Leu Val Ala Arg Ala Thr Ala Ala Gly Val 165 170 175 Ala Glu Asn Gln Ile Val Leu Asp Pro Gly Leu Gly Phe Ala Lys Ser 180 185 190 Arg Glu Asp Asn Trp Arg Leu Leu Gln Ala Leu Pro Glu Phe Ile Ser 195 200 205 Gly Pro Phe Pro Ile Leu Val Gly Ala Ser Arg Lys Arg Phe Leu Ala 210 215 220 Gly Val Arg Lys Asp Arg Gly Leu Asp Val Thr Pro Ile Asp Ala Asp 225 230 235 240 Pro Ala Thr Ala Ala Val Thr Ala Val Ser Ala His Met Gly Ala Trp 245 250 255 Gly Val Arg Val His Asp Val Pro Val Ser Arg Asp Ala Val Asp Val 260 265 270 Ala Ala Leu Trp Arg Ser Gly Gly Thr His His Gly 275 280 <210> 69 <211> 604 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(574) <223> RXA01518 <400> 69 agcagaacct gatgccgtcc tgcacggcac gaccattgca gaacatgtgg ataatcttga 60 tcccacagac attgaaggtg tcaccaagat ttaaggagtc gtg gct ttc atg cag 115 Val Ala Phe Met Gln 1 5 aaa acg tca gcg ggt tgg ttg atc gca acg gga ggt ttc ctc gcc gct 163 Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly Gly Phe Leu Ala Ala 10 15 20 gtg tcc gcc att ttg acg tgg cgt ttt tat gga tcc atg acc tct att 211 Val Ser Ala Ile Leu Thr Trp Arg Phe Tyr Gly Ser Met Thr Ser Ile 25 30 35 tcc atc acg gta tcc atc act ttt tgg ttg ttg gcc gtg gtg tgt ggt 259 Ser Ile Thr Val Ser Ile Thr Phe Trp Leu Leu Ala Val Val Cys Gly 40 45 50 ttc gca ggt gtg aag gtc caa ggt cgc ctc gat gag ggg ctg atc ggc 307 Phe Ala Gly Val Lys Val Gln Gly Arg Leu Asp Glu Gly Leu Ile Gly 55 60 65 cag gac aaa tcc caa atg aac ccc gtg acc att gcc tat ctg gcc atg 355 Gln Asp Lys Ser Gln Met Asn Pro Val Thr Ile Ala Tyr Leu Ala Met 70 75 80 85 ctg ggt cga gcg tgt gcg tgg ggt ggc gca att ttc ggc ggc gtt tat 403 Leu Gly Arg Ala Cys Ala Trp Gly Gly Ala Ile Phe Gly Gly Val Tyr 90 95 100 gtg gga att ggc agt tat gta atc cca cgc gcc ggt gag ttg tcc gca 451 Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala Gly Glu Leu Ser Ala 105 110 115 gca tcg aat gat ctt ccg gga gtt att gcc tgt gcg ctg ggc gga atc 499 Ala Ser Asn Asp Leu Pro Gly Val Ile Ala Cys Ala Leu Gly Gly Ile 120 125 130 gca ctc tca gct gcc gga ctt tat tta gag cga agc tgt gag gct ccg 547 Ala Leu Ser Ala Ala Gly Leu Tyr Leu Glu Arg Ser Cys Glu Ala Pro 135 140 145 cct ccc caa tct ggc gaa gcg atc agc tagattggaa ttcatgaatc 594 Pro Pro Gln Ser Gly Glu Ala Ile Ser 150 155 aagacctgtc 604 <210> 70 <211> 158 <212> PRT <213> Corynebacterium glutamicum <400> 70 Val Ala Phe Met Gln Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly 1 5 10 15 Gly Phe Leu Ala Ala Val Ser Ala Ile Leu Thr Trp Arg Phe Tyr Gly 20 25 30 Ser Met Thr Ser Ile Ser Ile Thr Val Ser Ile Thr Phe Trp Leu Leu 35 40 45 Ala Val Val Cys Gly Phe Ala Gly Val Lys Val Gln Gly Arg Leu Asp 50 55 60 Glu Gly Leu Ile Gly Gln Asp Lys Ser Gln Met Asn Pro Val Thr Ile 65 70 75 80 Ala Tyr Leu Ala Met Leu Gly Arg Ala Cys Ala Trp Gly Gly Ala Ile 85 90 95 Phe Gly Gly Val Tyr Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala 100 105 110 Gly Glu Leu Ser Ala Ala Ser Asn Asp Leu Pro Gly Val Ile Ala Cys 115 120 125 Ala Leu Gly Gly Ile Ala Leu Ser Ala Ala Gly Leu Tyr Leu Glu Arg 130 135 140 Ser Cys Glu Ala Pro Pro Pro Gln Ser Gly Glu Ala Ile Ser 145 150 155 <210> 71 <211> 1267 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1237) <223> RXA01690 <400> 71 gggggaggca tcaaattgaa actaactttt aacaagccta gccattcctc aaaaccgtga 60 gacgaaattg gctattcatc ccataaaatg gggctgacta gtg tat ctg tca ggt 115 Val Tyr Leu Ser Gly 1 5 agc agg tgt acc tta aaa tcc atg acg tca tta gag ttc aca gta acc 163 Ser Arg Cys Thr Leu Lys Ser Met Thr Ser Leu Glu Phe Thr Val Thr 10 15 20 cgt acc gaa aat ccg acg tca ccc gat cgt ctg aag gaa att ctt gcc 211 Arg Thr Glu Asn Pro Thr Ser Pro Asp Arg Leu Lys Glu Ile Leu Ala 25 30 35 gca ccg aag ttc ggt aag ttc ttc acc gac cac atg gtg acc att gac 259 Ala Pro Lys Phe Gly Lys Phe Phe Thr Asp His Met Val Thr Ile Asp 40 45 50 tgg aac gag tcg gaa ggc tgg cac aac gcc caa tta gtg cca tac gcg 307 Trp Asn Glu Ser Glu Gly Trp His Asn Ala Gln Leu Val Pro Tyr Ala 55 60 65 ccg att cct atg gat cct gcc acc acc gta ttc cac tac gga cag gca 355 Pro Ile Pro Met Asp Pro Ala Thr Thr Val Phe His Tyr Gly Gln Ala 70 75 80 85 att ttt gag gga att aag gcc tac cgc cat tcg gac gaa acc atc aag 403 Ile Phe Glu Gly Ile Lys Ala Tyr Arg His Ser Asp Glu Thr Ile Lys 90 95 100 act ttc cgt cct gat gaa aac gcc gag cgt atg cag cgt tca gca gct 451 Thr Phe Arg Pro Asp Glu Asn Ala Glu Arg Met Gln Arg Ser Ala Ala 105 110 115 cga atg gca atg cca cag ttg cca acc gag gac ttt att aaa gca ctt 499 Arg Met Ala Met Pro Gln Leu Pro Thr Glu Asp Phe Ile Lys Ala Leu 120 125 130 gaa ctg ctg gta gac gcg gat cag gat tgg gtt cct gag tac ggc gga 547 Glu Leu Leu Val Asp Ala Asp Gln Asp Trp Val Pro Glu Tyr Gly Gly 135 140 145 gaa gct tcc ctc tac ctg cgc cca ttc atg atc tcc acc gaa att ggc 595 Glu Ala Ser Leu Tyr Leu Arg Pro Phe Met Ile Ser Thr Glu Ile Gly 150 155 160 165 ttg ggt gtc agc cca gct gat gcc tac aag ttc ctg gtc atc gca tcc 643 Leu Gly Val Ser Pro Ala Asp Ala Tyr Lys Phe Leu Val Ile Ala Ser 170 175 180 cca gtc ggc gct tac ttc acc ggt gga atc aag cct gtt tcc gtc tgg 691 Pro Val Gly Ala Tyr Phe Thr Gly Gly Ile Lys Pro Val Ser Val Trp 185 190 195 ctg agc gaa gat tac gtc cgc gct gca ccc ggc gga act ggt gac gcc 739 Leu Ser Glu Asp Tyr Val Arg Ala Ala Pro Gly Gly Thr Gly Asp Ala 200 205 210 aaa ttt gct ggc aac tac gcg gct tct ttg ctt gcc cag tcc cag gct 787 Lys Phe Ala Gly Asn Tyr Ala Ala Ser Leu Leu Ala Gln Ser Gln Ala 215 220 225 gcg gaa aag ggc tgt gac cag gtc gta tgg ttg gat gcc atc gag cac 835 Ala Glu Lys Gly Cys Asp Gln Val Val Trp Leu Asp Ala Ile Glu His 230 235 240 245 aag tac atc gaa gaa atg ggt ggc atg aac ctt ggg ttc atc tac cgc 883 Lys Tyr Ile Glu Glu Met Gly Gly Met Asn Leu Gly Phe Ile Tyr Arg 250 255 260 aac ggc gac caa gtc aag cta gtc acc cct gaa ctt tcc ggc tca cta 931 Asn Gly Asp Gln Val Lys Leu Val Thr Pro Glu Leu Ser Gly Ser Leu 265 270 275 ctt cca ggc atc acc cgc aag tca ctt cta caa gta gca cgc gac ttg 979 Leu Pro Gly Ile Thr Arg Lys Ser Leu Leu Gln Val Ala Arg Asp Leu 280 285 290 gga tac gaa gta gaa gag cga aag atc acc acc acc gag tgg gaa gaa 1027 Gly Tyr Glu Val Glu Glu Arg Lys Ile Thr Thr Thr Glu Trp Glu Glu 295 300 305 gac gca aag tct ggc gcc atg acc gag gca ttt gct tgc ggt act gca 1075 Asp Ala Lys Ser Gly Ala Met Thr Glu Ala Phe Ala Cys Gly Thr Ala 310 315 320 325 gct gtt atc acc cct gtt ggc acc gtg aaa tca gct cac ggc acc ttc 1123 Ala Val Ile Thr Pro Val Gly Thr Val Lys Ser Ala His Gly Thr Phe 330 335 340 gaa gtg aac aac aat gaa gtc gga gaa atc acg atg aag ctt cgt gaa 1171 Glu Val Asn Asn Asn Glu Val Gly Glu Ile Thr Met Lys Leu Arg Glu 345 350 355 acc ctc acc gga att cag caa gga aac gtt gaa gac caa aac gga tgg 1219 Thr Leu Thr Gly Ile Gln Gln Gly Asn Val Glu Asp Gln Asn Gly Trp 360 365 370 ctt tac cca ctg gtt ggc taaatcaacc ggttttaaga ccccgctgca 1267 Leu Tyr Pro Leu Val Gly 375 <210> 72 <211> 379 <212> PRT <213> Corynebacterium glutamicum <400> 72 Val Tyr Leu Ser Gly Ser Arg Cys Thr Leu Lys Ser Met Thr Ser Leu 1 5 10 15 Glu Phe Thr Val Thr Arg Thr Glu Asn Pro Thr Ser Pro Asp Arg Leu 20 25 30 Lys Glu Ile Leu Ala Ala Pro Lys Phe Gly Lys Phe Phe Thr Asp His 35 40 45 Met Val Thr Ile Asp Trp Asn Glu Ser Glu Gly Trp His Asn Ala Gln 50 55 60 Leu Val Pro Tyr Ala Pro Ile Pro Met Asp Pro Ala Thr Thr Val Phe 65 70 75 80 His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala Tyr Arg His Ser 85 90 95 Asp Glu Thr Ile Lys Thr Phe Arg Pro Asp Glu Asn Ala Glu Arg Met 100 105 110 Gln Arg Ser Ala Ala Arg Met Ala Met Pro Gln Leu Pro Thr Glu Asp 115 120 125 Phe Ile Lys Ala Leu Glu Leu Leu Val Asp Ala Asp Gln Asp Trp Val 130 135 140 Pro Glu Tyr Gly Gly Glu Ala Ser Leu Tyr Leu Arg Pro Phe Met Ile 145 150 155 160 Ser Thr Glu Ile Gly Leu Gly Val Ser Pro Ala Asp Ala Tyr Lys Phe 165 170 175 Leu Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr Gly Gly Ile Lys 180 185 190 Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg Ala Ala Pro Gly 195 200 205 Gly Thr Gly Asp Ala Lys Phe Ala Gly Asn Tyr Ala Ala Ser Leu Leu 210 215 220 Ala Gln Ser Gln Ala Ala Glu Lys Gly Cys Asp Gln Val Val Trp Leu 225 230 235 240 Asp Ala Ile Glu His Lys Tyr Ile Glu Glu Met Gly Gly Met Asn Leu 245 250 255 Gly Phe Ile Tyr Arg Asn Gly Asp Gln Val Lys Leu Val Thr Pro Glu 260 265 270 Leu Ser Gly Ser Leu Leu Pro Gly Ile Thr Arg Lys Ser Leu Leu Gln 275 280 285 Val Ala Arg Asp Leu Gly Tyr Glu Val Glu Glu Arg Lys Ile Thr Thr 290 295 300 Thr Glu Trp Glu Glu Asp Ala Lys Ser Gly Ala Met Thr Glu Ala Phe 305 310 315 320 Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly Thr Val Lys Ser 325 330 335 Ala His Gly Thr Phe Glu Val Asn Asn Asn Glu Val Gly Glu Ile Thr 340 345 350 Met Lys Leu Arg Glu Thr Leu Thr Gly Ile Gln Gln Gly Asn Val Glu 355 360 365 Asp Gln Asn Gly Trp Leu Tyr Pro Leu Val Gly 370 375 <210> 73 <211> 910 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(880) <223> RXA01746 <400> 73 ctgcagctct aagatctctg caagttaaaa ccgccactcc cctttcactg gggagtggcg 60 gttttgtcgt ttcatgcatg cagtgtgtga cttatcaacc ttg tta ggg cta ggg 115 Leu Leu Gly Leu Gly 1 5 tgg ata tct atc atg act gca cca aga gat cct ttt ttc ccc gca gat 163 Trp Ile Ser Ile Met Thr Ala Pro Arg Asp Pro Phe Phe Pro Ala Asp 10 15 20 ctt tct atc cgc gcg tct gca gag ccc att gaa att cag cgg ttg ggt 211 Leu Ser Ile Arg Ala Ser Ala Glu Pro Ile Glu Ile Gln Arg Leu Gly 25 30 35 ttg atc gat tat caa gag gcc tgg gat tat caa gca gag ctt gct acc 259 Leu Ile Asp Tyr Gln Glu Ala Trp Asp Tyr Gln Ala Glu Leu Ala Thr 40 45 50 cgt agg gct aat gat gaa atc cct gat cag ctg ctt att ttg gag cac 307 Arg Arg Ala Asn Asp Glu Ile Pro Asp Gln Leu Leu Ile Leu Glu His 55 60 65 ccg tcg gtg tat acc gca ggt aag cgc acc cag ccg gaa gat ctt ccc 355 Pro Ser Val Tyr Thr Ala Gly Lys Arg Thr Gln Pro Glu Asp Leu Pro 70 75 80 85 acc aac gga ctg ccg gtg atc aat gct gat cgt ggt ggt cgc atc acg 403 Thr Asn Gly Leu Pro Val Ile Asn Ala Asp Arg Gly Gly Arg Ile Thr 90 95 100 tgg cat ggt cct ggc caa ttg gtg atc tat ccg atc atc aaa tta gcc 451 Trp His Gly Pro Gly Gln Leu Val Ile Tyr Pro Ile Ile Lys Leu Ala 105 110 115 gat ccg atc gat gtg gtt gat tac gta aga cgc ctc gag gaa gcg ctc 499 Asp Pro Ile Asp Val Val Asp Tyr Val Arg Arg Leu Glu Glu Ala Leu 120 125 130 atc caa gtt gtc ggc gat atg ggt gtt gcc ggc gct ggg cgc att gat 547 Ile Gln Val Val Gly Asp Met Gly Val Ala Gly Ala Gly Arg Ile Asp 135 140 145 ggg cgt tcg ggt gtg tgg gtg cca gct cat gat ggt tgg gtg gac agc 595 Gly Arg Ser Gly Val Trp Val Pro Ala His Asp Gly Trp Val Asp Ser 150 155 160 165 aag gtt gcg gcc atc ggc att cga ata act cgt ggt gtt gca atg cac 643 Lys Val Ala Ala Ile Gly Ile Arg Ile Thr Arg Gly Val Ala Met His 170 175 180 ggt gtg gcc atc aac tgc aac aac acg ttg gat ttc tat gag cac atc 691 Gly Val Ala Ile Asn Cys Asn Asn Thr Leu Asp Phe Tyr Glu His Ile 185 190 195 att ccg tgt ggc att gct gat gca ggc ttg agc aca ctc tcg agg gaa 739 Ile Pro Cys Gly Ile Ala Asp Ala Gly Leu Ser Thr Leu Ser Arg Glu 200 205 210 ctg aaa agg gac gtt tca gtt gag gaa tta gtc gag cca tcg atc cgc 787 Leu Lys Arg Asp Val Ser Val Glu Glu Leu Val Glu Pro Ser Ile Arg 215 220 225 gca ttg gat gat gct ttg gct ggt cgg ctg gtt gtt tct gat cat tct 835 Ala Leu Asp Asp Ala Leu Ala Gly Arg Leu Val Val Ser Asp His Ser 230 235 240 245 ttc ggc agc gcg ccc gac cca act aag aat ctc cct aaa cgg ggg 880 Phe Gly Ser Ala Pro Asp Pro Thr Lys Asn Leu Pro Lys Arg Gly 250 255 260 tagtacgagg aattttgtcg gtggggcgcc 910 <210> 74 <211> 260 <212> PRT <213> Corynebacterium glutamicum <400> 74 Leu Leu Gly Leu Gly Trp Ile Ser Ile Met Thr Ala Pro Arg Asp Pro 1 5 10 15 Phe Phe Pro Ala Asp Leu Ser Ile Arg Ala Ser Ala Glu Pro Ile Glu 20 25 30 Ile Gln Arg Leu Gly Leu Ile Asp Tyr Gln Glu Ala Trp Asp Tyr Gln 35 40 45 Ala Glu Leu Ala Thr Arg Arg Ala Asn Asp Glu Ile Pro Asp Gln Leu 50 55 60 Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg Thr Gln 65 70 75 80 Pro Glu Asp Leu Pro Thr Asn Gly Leu Pro Val Ile Asn Ala Asp Arg 85 90 95 Gly Gly Arg Ile Thr Trp His Gly Pro Gly Gln Leu Val Ile Tyr Pro 100 105 110 Ile Ile Lys Leu Ala Asp Pro Ile Asp Val Val Asp Tyr Val Arg Arg 115 120 125 Leu Glu Glu Ala Leu Ile Gln Val Val Gly Asp Met Gly Val Ala Gly 130 135 140 Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala His Asp 145 150 155 160 Gly Trp Val Asp Ser Lys Val Ala Ala Ile Gly Ile Arg Ile Thr Arg 165 170 175 Gly Val Ala Met His Gly Val Ala Ile Asn Cys Asn Asn Thr Leu Asp 180 185 190 Phe Tyr Glu His Ile Ile Pro Cys Gly Ile Ala Asp Ala Gly Leu Ser 195 200 205 Thr Leu Ser Arg Glu Leu Lys Arg Asp Val Ser Val Glu Glu Leu Val 210 215 220 Glu Pro Ser Ile Arg Ala Leu Asp Asp Ala Leu Ala Gly Arg Leu Val 225 230 235 240 Val Ser Asp His Ser Phe Gly Ser Ala Pro Asp Pro Thr Lys Asn Leu 245 250 255 Pro Lys Arg Gly 260 <210> 75 <211> 1207 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1177) <223> RXA01810 <400> 75 ccaccaccac aatgttgctc atcctcgcgg cgttcgttgt cgcaggtggc tccatgactc 60 gcttcaccgt cggcaacccg actggaaaat aaggcttcac atg aat aac gct ttt 115 Met Asn Asn Ala Phe 1 5 cga cgc acc ctt aca tcc gta gtc ctc gcc gct agc ttg gcc tta acg 163 Arg Arg Thr Leu Thr Ser Val Val Leu Ala Ala Ser Leu Ala Leu Thr 10 15 20 gcc tgc gca agc tgg gat tca cct acg gca tct tcc aat ggt gat ctg 211 Ala Cys Ala Ser Trp Asp Ser Pro Thr Ala Ser Ser Asn Gly Asp Leu 25 30 35 att gag gag atc cag gca agc tcc acc tca aca gat ccg cgc acc ttc 259 Ile Glu Glu Ile Gln Ala Ser Ser Thr Ser Thr Asp Pro Arg Thr Phe 40 45 50 aca ggc ttg agc atc gtg gaa gat atc ggc gat gtg gtt ccc gta acc 307 Thr Gly Leu Ser Ile Val Glu Asp Ile Gly Asp Val Val Pro Val Thr 55 60 65 gac aac gcc tca cca gct ctg cca gtt tct ttg acc gac gct gat ggc 355 Asp Asn Ala Ser Pro Ala Leu Pro Val Ser Leu Thr Asp Ala Asp Gly 70 75 80 85 aac gac gtg gtg gtg gag gac gtg tcc cgc atc ctc cca ctg gat ctc 403 Asn Asp Val Val Val Glu Asp Val Ser Arg Ile Leu Pro Leu Asp Leu 90 95 100 tac gga acc tat tcc aaa acc atc gct ggc ctg gga ctc gtg gac aat 451 Tyr Gly Thr Tyr Ser Lys Thr Ile Ala Gly Leu Gly Leu Val Asp Asn 105 110 115 att gtg ggt cgt act gtt agt tcc acc gag cct gca ttg gcg gac act 499 Ile Val Gly Arg Thr Val Ser Ser Thr Glu Pro Ala Leu Ala Asp Thr 120 125 130 gag gtg gtc acc act ggc gga cac acc ctc aat gct gaa gcg atc ctt 547 Glu Val Val Thr Thr Gly Gly His Thr Leu Asn Ala Glu Ala Ile Leu 135 140 145 aat tta cat ccg act ttg gtg atc atc gac cac tcg atc ggc cca cgc 595 Asn Leu His Pro Thr Leu Val Ile Ile Asp His Ser Ile Gly Pro Arg 150 155 160 165 gaa gtc atc gat cag atc cgc gca gct ggt gtc gcc acg gtg atc atg 643 Glu Val Ile Asp Gln Ile Arg Ala Ala Gly Val Ala Thr Val Ile Met 170 175 180 tcg ccg cag cgt tcc att gcc tca att ggc gac gac atc cgc gac atc 691 Ser Pro Gln Arg Ser Ile Ala Ser Ile Gly Asp Asp Ile Arg Asp Ile 185 190 195 gcc tcc gtc gtt gga ctt cct gaa gaa ggg gag aag ctc gcg gaa cgt 739 Ala Ser Val Val Gly Leu Pro Glu Glu Gly Glu Lys Leu Ala Glu Arg 200 205 210 tcc gtt gct gaa gtc gaa gag gcc agc acg gtt gtc gat gaa ctc acc 787 Ser Val Ala Glu Val Glu Glu Ala Ser Thr Val Val Asp Glu Leu Thr 215 220 225 cca gaa gat ccc ctc aaa atg gta ttc ctc tat gcc cgc gga act ggt 835 Pro Glu Asp Pro Leu Lys Met Val Phe Leu Tyr Ala Arg Gly Thr Gly 230 235 240 245 gga gtg ttc ttc att ttg ggc gat gcc tat ggt gga cgc gat ctc att 883 Gly Val Phe Phe Ile Leu Gly Asp Ala Tyr Gly Gly Arg Asp Leu Ile 250 255 260 gaa ggc ctg ggc ggc gtc gac atg gct gct gaa aag ggc atc atg gat 931 Glu Gly Leu Gly Gly Val Asp Met Ala Ala Glu Lys Gly Ile Met Asp 265 270 275 ctg gca cca gcc aac gcg gaa gca ctt gcc gaa cta aat cca gac gtc 979 Leu Ala Pro Ala Asn Ala Glu Ala Leu Ala Glu Leu Asn Pro Asp Val 280 285 290 ttc gtg atg atg tcg gaa gga cta gtc tcg aca gga ggt atc gac ggt 1027 Phe Val Met Met Ser Glu Gly Leu Val Ser Thr Gly Gly Ile Asp Gly 295 300 305 ctt atg gaa cgc ccc ggc att gct cag aca acc gcc gga caa aac caa 1075 Leu Met Glu Arg Pro Gly Ile Ala Gln Thr Thr Ala Gly Gln Asn Gln 310 315 320 325 cga gta ctg gcg ctt ccc gat ggt caa tca ttg gcc ttt ggt gcc caa 1123 Arg Val Leu Ala Leu Pro Asp Gly Gln Ser Leu Ala Phe Gly Ala Gln 330 335 340 act ggc gag ttg ttg ctc cgc gca tcc cgc gaa ctg tat gtg cag ggc 1171 Thr Gly Glu Leu Leu Leu Arg Ala Ser Arg Glu Leu Tyr Val Gln Gly 345 350 355 ggc gag tagatggttg tgaaggaggt tgacgtcgaa 1207 Gly Glu <210> 76 <211> 359 <212> PRT <213> Corynebacterium glutamicum <400> 76 Met Asn Asn Ala Phe Arg Arg Thr Leu Thr Ser Val Val Leu Ala Ala 1 5 10 15 Ser Leu Ala Leu Thr Ala Cys Ala Ser Trp Asp Ser Pro Thr Ala Ser 20 25 30 Ser Asn Gly Asp Leu Ile Glu Glu Ile Gln Ala Ser Ser Thr Ser Thr 35 40 45 Asp Pro Arg Thr Phe Thr Gly Leu Ser Ile Val Glu Asp Ile Gly Asp 50 55 60 Val Val Pro Val Thr Asp Asn Ala Ser Pro Ala Leu Pro Val Ser Leu 65 70 75 80 Thr Asp Ala Asp Gly Asn Asp Val Val Val Glu Asp Val Ser Arg Ile 85 90 95 Leu Pro Leu Asp Leu Tyr Gly Thr Tyr Ser Lys Thr Ile Ala Gly Leu 100 105 110 Gly Leu Val Asp Asn Ile Val Gly Arg Thr Val Ser Ser Thr Glu Pro 115 120 125 Ala Leu Ala Asp Thr Glu Val Val Thr Thr Gly Gly His Thr Leu Asn 130 135 140 Ala Glu Ala Ile Leu Asn Leu His Pro Thr Leu Val Ile Ile Asp His 145 150 155 160 Ser Ile Gly Pro Arg Glu Val Ile Asp Gln Ile Arg Ala Ala Gly Val 165 170 175 Ala Thr Val Ile Met Ser Pro Gln Arg Ser Ile Ala Ser Ile Gly Asp 180 185 190 Asp Ile Arg Asp Ile Ala Ser Val Val Gly Leu Pro Glu Glu Gly Glu 195 200 205 Lys Leu Ala Glu Arg Ser Val Ala Glu Val Glu Glu Ala Ser Thr Val 210 215 220 Val Asp Glu Leu Thr Pro Glu Asp Pro Leu Lys Met Val Phe Leu Tyr 225 230 235 240 Ala Arg Gly Thr Gly Gly Val Phe Phe Ile Leu Gly Asp Ala Tyr Gly 245 250 255 Gly Arg Asp Leu Ile Glu Gly Leu Gly Gly Val Asp Met Ala Ala Glu 260 265 270 Lys Gly Ile Met Asp Leu Ala Pro Ala Asn Ala Glu Ala Leu Ala Glu 275 280 285 Leu Asn Pro Asp Val Phe Val Met Met Ser Glu Gly Leu Val Ser Thr 290 295 300 Gly Gly Ile Asp Gly Leu Met Glu Arg Pro Gly Ile Ala Gln Thr Thr 305 310 315 320 Ala Gly Gln Asn Gln Arg Val Leu Ala Leu Pro Asp Gly Gln Ser Leu 325 330 335 Ala Phe Gly Ala Gln Thr Gly Glu Leu Leu Leu Arg Ala Ser Arg Glu 340 345 350 Leu Tyr Val Gln Gly Gly Glu 355 <210> 77 <211> 1582 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1552) <223> RXA01850 <400> 77 caaaccggcc atcgcccttt ggaatcggtc cgaaaattgc aggtacagag ccttttaccg 60 agaaaatcca ccacagattg ctgaaatttc gtgatctgtg gtg gat tcg tgc aac 115 Val Asp Ser Cys Asn 1 5 ttc aga ctc tta cgg agg cga tgg acc aaa aac aac tac aat caa gca 163 Phe Arg Leu Leu Arg Arg Arg Trp Thr Lys Asn Asn Tyr Asn Gln Ala 10 15 20 gat cac ctt gta cac cac cat aga aaa ggc cca ccc tca gcc atg gct 211 Asp His Leu Val His His His Arg Lys Gly Pro Pro Ser Ala Met Ala 25 30 35 atc agt gtt gtt gat cta ttt agc atc ggt atc gga cca tca tcc tca 259 Ile Ser Val Val Asp Leu Phe Ser Ile Gly Ile Gly Pro Ser Ser Ser 40 45 50 cat acc gtc ggc ccc atg aga gcc gcc ctc acg tat atc tct gaa ttt 307 His Thr Val Gly Pro Met Arg Ala Ala Leu Thr Tyr Ile Ser Glu Phe 55 60 65 ccc agc tcg cat gtc gat atc acg ttg cac gga tcc ctt gcc gcc acc 355 Pro Ser Ser His Val Asp Ile Thr Leu His Gly Ser Leu Ala Ala Thr 70 75 80 85 ggt aaa ggc cac tgc act gac cgg gcg gta tta ctg ggt ctg gtg gga 403 Gly Lys Gly His Cys Thr Asp Arg Ala Val Leu Leu Gly Leu Val Gly 90 95 100 tgg gaa cca acg ata gtt ccc att gat gct gca ccc tca ccc ggc gcg 451 Trp Glu Pro Thr Ile Val Pro Ile Asp Ala Ala Pro Ser Pro Gly Ala 105 110 115 ccg att cct gcg aaa ggt tct gtg aac ggg cca aag gga acg gtg tcg 499 Pro Ile Pro Ala Lys Gly Ser Val Asn Gly Pro Lys Gly Thr Val Ser 120 125 130 tat tcc ctg acg ttt gat cct cat cct ctt cca gaa cac ccc aat gcc 547 Tyr Ser Leu Thr Phe Asp Pro His Pro Leu Pro Glu His Pro Asn Ala 135 140 145 gtt acc ttt aaa gga tca acc aca agg act tat ttg tcg gtg ggt ggt 595 Val Thr Phe Lys Gly Ser Thr Thr Arg Thr Tyr Leu Ser Val Gly Gly 150 155 160 165 ggg ttc att atg acg ttg gag gat ttc cgg aag ctg gac gat atc gga 643 Gly Phe Ile Met Thr Leu Glu Asp Phe Arg Lys Leu Asp Asp Ile Gly 170 175 180 tca ggt gtg tca acc att cat cca gag gca gag gtg cct tgt cct ttt 691 Ser Gly Val Ser Thr Ile His Pro Glu Ala Glu Val Pro Cys Pro Phe 185 190 195 cag aag agt tcc caa tta ctc gca tat ggt cgc gat ttt gcg gag gtc 739 Gln Lys Ser Ser Gln Leu Leu Ala Tyr Gly Arg Asp Phe Ala Glu Val 200 205 210 atg aag gat aat gag cgc tta atc cac ggg gat ctt ggc aca gtg gat 787 Met Lys Asp Asn Glu Arg Leu Ile His Gly Asp Leu Gly Thr Val Asp 215 220 225 gcc cat ttg gat cga gtg tgg cag att atg cag gag tgc gtg gca caa 835 Ala His Leu Asp Arg Val Trp Gln Ile Met Gln Glu Cys Val Ala Gln 230 235 240 245 ggc atc gca acg ccg ggg att tta ccg ggt ggg ttg aat gtg caa cgt 883 Gly Ile Ala Thr Pro Gly Ile Leu Pro Gly Gly Leu Asn Val Gln Arg 250 255 260 cgg gcg ccg cag gta cac gcg ctg att agc aac ggg gat acg tgt gag 931 Arg Ala Pro Gln Val His Ala Leu Ile Ser Asn Gly Asp Thr Cys Glu 265 270 275 ctg ggt gct gat ctt gat gct gtg gag tgg gtg aat ctg tac gcc ttg 979 Leu Gly Ala Asp Leu Asp Ala Val Glu Trp Val Asn Leu Tyr Ala Leu 280 285 290 gcg gtg aat gaa gaa aac gcc gct ggt ggt cgt gtg gtt act gct ccg 1027 Ala Val Asn Glu Glu Asn Ala Ala Gly Gly Arg Val Val Thr Ala Pro 295 300 305 act aat ggt gct gcg ggg att att ccg gcg gtg atg cac tat gcg cgg 1075 Thr Asn Gly Ala Ala Gly Ile Ile Pro Ala Val Met His Tyr Ala Arg 310 315 320 325 gat ttt ttg aca ggt ttt ggg gcg gag cag gcg cgg acg ttt ttg tat 1123 Asp Phe Leu Thr Gly Phe Gly Ala Glu Gln Ala Arg Thr Phe Leu Tyr 330 335 340 acc gcg ggt gcg gtg ggc atc atc att aag gaa aat gcc tcg atc tct 1171 Thr Ala Gly Ala Val Gly Ile Ile Ile Lys Glu Asn Ala Ser Ile Ser 345 350 355 ggc gcg gag gtg ggg tgt cag ggt gag gtt ggt tca gcg tcc gcg atg 1219 Gly Ala Glu Val Gly Cys Gln Gly Glu Val Gly Ser Ala Ser Ala Met 360 365 370 gcg gct gcc ggg ttg tgt gca gtc tta ggt ggt tct ccg caa cag gtg 1267 Ala Ala Ala Gly Leu Cys Ala Val Leu Gly Gly Ser Pro Gln Gln Val 375 380 385 gaa aac gcc gcg gag att gcg ttg gag cac aat ttg gga ttg acg tgc 1315 Glu Asn Ala Ala Glu Ile Ala Leu Glu His Asn Leu Gly Leu Thr Cys 390 395 400 405 gat ccg gtg ggc ggg tta gtg cag att ccg tgt att gaa cgc aac gct 1363 Asp Pro Val Gly Gly Leu Val Gln Ile Pro Cys Ile Glu Arg Asn Ala 410 415 420 att gct gcc atg aag tcc atc aat gcg gca agg ctt gcc cgg att ggt 1411 Ile Ala Ala Met Lys Ser Ile Asn Ala Ala Arg Leu Ala Arg Ile Gly 425 430 435 gat ggc aac aat cgc gtg agt ttg gat gat gtg gtg gtc acg atg gct 1459 Asp Gly Asn Asn Arg Val Ser Leu Asp Asp Val Val Val Thr Met Ala 440 445 450 gcc acc ggc cgg gac atg ctg acc aaa tat aag gaa acg tcc ctt ggt 1507 Ala Thr Gly Arg Asp Met Leu Thr Lys Tyr Lys Glu Thr Ser Leu Gly 455 460 465 ggt ttg gca acc acc ttg ggc ttc ccg gtg tcg atg acg gag tgt 1552 Gly Leu Ala Thr Thr Leu Gly Phe Pro Val Ser Met Thr Glu Cys 470 475 480 tagcggtacg gctttaacac ggcttggatt 1582 <210> 78 <211> 484 <212> PRT <213> Corynebacterium glutamicum <400> 78 Val Asp Ser Cys Asn Phe Arg Leu Leu Arg Arg Arg Trp Thr Lys Asn 1 5 10 15 Asn Tyr Asn Gln Ala Asp His Leu Val His His His Arg Lys Gly Pro 20 25 30 Pro Ser Ala Met Ala Ile Ser Val Val Asp Leu Phe Ser Ile Gly Ile 35 40 45 Gly Pro Ser Ser Ser His Thr Val Gly Pro Met Arg Ala Ala Leu Thr 50 55 60 Tyr Ile Ser Glu Phe Pro Ser Ser His Val Asp Ile Thr Leu His Gly 65 70 75 80 Ser Leu Ala Ala Thr Gly Lys Gly His Cys Thr Asp Arg Ala Val Leu 85 90 95 Leu Gly Leu Val Gly Trp Glu Pro Thr Ile Val Pro Ile Asp Ala Ala 100 105 110 Pro Ser Pro Gly Ala Pro Ile Pro Ala Lys Gly Ser Val Asn Gly Pro 115 120 125 Lys Gly Thr Val Ser Tyr Ser Leu Thr Phe Asp Pro His Pro Leu Pro 130 135 140 Glu His Pro Asn Ala Val Thr Phe Lys Gly Ser Thr Thr Arg Thr Tyr 145 150 155 160 Leu Ser Val Gly Gly Gly Phe Ile Met Thr Leu Glu Asp Phe Arg Lys 165 170 175 Leu Asp Asp Ile Gly Ser Gly Val Ser Thr Ile His Pro Glu Ala Glu 180 185 190 Val Pro Cys Pro Phe Gln Lys Ser Ser Gln Leu Leu Ala Tyr Gly Arg 195 200 205 Asp Phe Ala Glu Val Met Lys Asp Asn Glu Arg Leu Ile His Gly Asp 210 215 220 Leu Gly Thr Val Asp Ala His Leu Asp Arg Val Trp Gln Ile Met Gln 225 230 235 240 Glu Cys Val Ala Gln Gly Ile Ala Thr Pro Gly Ile Leu Pro Gly Gly 245 250 255 Leu Asn Val Gln Arg Arg Ala Pro Gln Val His Ala Leu Ile Ser Asn 260 265 270 Gly Asp Thr Cys Glu Leu Gly Ala Asp Leu Asp Ala Val Glu Trp Val 275 280 285 Asn Leu Tyr Ala Leu Ala Val Asn Glu Glu Asn Ala Ala Gly Gly Arg 290 295 300 Val Val Thr Ala Pro Thr Asn Gly Ala Ala Gly Ile Ile Pro Ala Val 305 310 315 320 Met His Tyr Ala Arg Asp Phe Leu Thr Gly Phe Gly Ala Glu Gln Ala 325 330 335 Arg Thr Phe Leu Tyr Thr Ala Gly Ala Val Gly Ile Ile Ile Lys Glu 340 345 350 Asn Ala Ser Ile Ser Gly Ala Glu Val Gly Cys Gln Gly Glu Val Gly 355 360 365 Ser Ala Ser Ala Met Ala Ala Ala Gly Leu Cys Ala Val Leu Gly Gly 370 375 380 Ser Pro Gln Gln Val Glu Asn Ala Ala Glu Ile Ala Leu Glu His Asn 385 390 395 400 Leu Gly Leu Thr Cys Asp Pro Val Gly Gly Leu Val Gln Ile Pro Cys 405 410 415 Ile Glu Arg Asn Ala Ile Ala Ala Met Lys Ser Ile Asn Ala Ala Arg 420 425 430 Leu Ala Arg Ile Gly Asp Gly Asn Asn Arg Val Ser Leu Asp Asp Val 435 440 445 Val Val Thr Met Ala Ala Thr Gly Arg Asp Met Leu Thr Lys Tyr Lys 450 455 460 Glu Thr Ser Leu Gly Gly Leu Ala Thr Thr Leu Gly Phe Pro Val Ser 465 470 475 480 Met Thr Glu Cys <210> 79 <211> 1066 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1036) <223> RXA01940 <400> 79 ccagaatcaa tcaccgatac agtggcagcc aaagtccagg ctgaagaagc cgctcaagcc 60 gccagctaaa tccacaaact gaactaagga gttttaccct atg acc acc aag atc 115 Met Thr Thr Lys Ile 1 5 atc ctc gac tgc gat cca gga cac gac gac gct gta gcc atg ctg ctc 163 Ile Leu Asp Cys Asp Pro Gly His Asp Asp Ala Val Ala Met Leu Leu 10 15 20 gca gcc ggc agc cca gaa att gaa ctg ctt gga atc acc acg gtc ggc 211 Ala Ala Gly Ser Pro Glu Ile Glu Leu Leu Gly Ile Thr Thr Val Gly 25 30 35 ggc aac cag acc ttg gac aag gtc acc cac aat acg cag gtc gta gcc 259 Gly Asn Gln Thr Leu Asp Lys Val Thr His Asn Thr Gln Val Val Ala 40 45 50 acc atc gct gat atc aat gcg ccc atc tac cgc ggt gtc acc cga cca 307 Thr Ile Ala Asp Ile Asn Ala Pro Ile Tyr Arg Gly Val Thr Arg Pro 55 60 65 ttg gtg cgc ccc gtt gag gta gcc gaa gat atc cac ggc gat acc ggc 355 Leu Val Arg Pro Val Glu Val Ala Glu Asp Ile His Gly Asp Thr Gly 70 75 80 85 atg gaa atc cac aag tac gaa ctg cct gaa cca acc aag cag gta gaa 403 Met Glu Ile His Lys Tyr Glu Leu Pro Glu Pro Thr Lys Gln Val Glu 90 95 100 gac acc cac gcg gtg gat ttc atc atc gat acc atc atg aat aac gag 451 Asp Thr His Ala Val Asp Phe Ile Ile Asp Thr Ile Met Asn Asn Glu 105 110 115 ccc ggc agc gta gcg ctg gtt ccc acc gga cca ctg acc aac atc gcg 499 Pro Gly Ser Val Ala Leu Val Pro Thr Gly Pro Leu Thr Asn Ile Ala 120 125 130 ctg gca gtc cgg aaa gaa cca cgc atc gcc gag cga gtc aag gaa gtt 547 Leu Ala Val Arg Lys Glu Pro Arg Ile Ala Glu Arg Val Lys Glu Val 135 140 145 gtc ctc atg ggc ggg ggc tac cac gta gga aac tgg acc gcc gta gct 595 Val Leu Met Gly Gly Gly Tyr His Val Gly Asn Trp Thr Ala Val Ala 150 155 160 165 gaa ttc aac atc aag atc gac ccc gaa gca gcc cac atc gta ttc aac 643 Glu Phe Asn Ile Lys Ile Asp Pro Glu Ala Ala His Ile Val Phe Asn 170 175 180 gaa aag tgg cca ctg act atg gtc ggc ctc gac ctt acc cac cag gcg 691 Glu Lys Trp Pro Leu Thr Met Val Gly Leu Asp Leu Thr His Gln Ala 185 190 195 ctc gca aca cct gag atc gaa gcc aag ttc aac gag ctg ggc acc gac 739 Leu Ala Thr Pro Glu Ile Glu Ala Lys Phe Asn Glu Leu Gly Thr Asp 200 205 210 gtc gcc gac ttc gtc gtc gcg ctt ttc gac gct ttc cgc aag aat tac 787 Val Ala Asp Phe Val Val Ala Leu Phe Asp Ala Phe Arg Lys Asn Tyr 215 220 225 cag gac gca cag ggt ttt gat aac cca cca gta cac gac cct tgt gct 835 Gln Asp Ala Gln Gly Phe Asp Asn Pro Pro Val His Asp Pro Cys Ala 230 235 240 245 gtt gca tac ctt gtt gac cca acc gta ttc acc acc cgc aaa gca cca 883 Val Ala Tyr Leu Val Asp Pro Thr Val Phe Thr Thr Arg Lys Ala Pro 250 255 260 ctc gat gtg gag ctg tac ggc gca ctc acc aca ggc atg acc gtt gct 931 Leu Asp Val Glu Leu Tyr Gly Ala Leu Thr Thr Gly Met Thr Val Ala 265 270 275 gat ttc cgc gca ccg gct cca gca gat tgc acc acc caa gta gct gtt 979 Asp Phe Arg Ala Pro Ala Pro Ala Asp Cys Thr Thr Gln Val Ala Val 280 285 290 gac ctg gac ttt gat aaa ttc tgg aac atg gtg atc gat gca gta aag 1027 Asp Leu Asp Phe Asp Lys Phe Trp Asn Met Val Ile Asp Ala Val Lys 295 300 305 cgc atc gga tagacctgtt cacaaggttg ttagagggcg 1066 Arg Ile Gly 310 <210> 80 <211> 312 <212> PRT <213> Corynebacterium glutamicum <400> 80 Met Thr Thr Lys Ile Ile Leu Asp Cys Asp Pro Gly His Asp Asp Ala 1 5 10 15 Val Ala Met Leu Leu Ala Ala Gly Ser Pro Glu Ile Glu Leu Leu Gly 20 25 30 Ile Thr Thr Val Gly Gly Asn Gln Thr Leu Asp Lys Val Thr His Asn 35 40 45 Thr Gln Val Val Ala Thr Ile Ala Asp Ile Asn Ala Pro Ile Tyr Arg 50 55 60 Gly Val Thr Arg Pro Leu Val Arg Pro Val Glu Val Ala Glu Asp Ile 65 70 75 80 His Gly Asp Thr Gly Met Glu Ile His Lys Tyr Glu Leu Pro Glu Pro 85 90 95 Thr Lys Gln Val Glu Asp Thr His Ala Val Asp Phe Ile Ile Asp Thr 100 105 110 Ile Met Asn Asn Glu Pro Gly Ser Val Ala Leu Val Pro Thr Gly Pro 115 120 125 Leu Thr Asn Ile Ala Leu Ala Val Arg Lys Glu Pro Arg Ile Ala Glu 130 135 140 Arg Val Lys Glu Val Val Leu Met Gly Gly Gly Tyr His Val Gly Asn 145 150 155 160 Trp Thr Ala Val Ala Glu Phe Asn Ile Lys Ile Asp Pro Glu Ala Ala 165 170 175 His Ile Val Phe Asn Glu Lys Trp Pro Leu Thr Met Val Gly Leu Asp 180 185 190 Leu Thr His Gln Ala Leu Ala Thr Pro Glu Ile Glu Ala Lys Phe Asn 195 200 205 Glu Leu Gly Thr Asp Val Ala Asp Phe Val Val Ala Leu Phe Asp Ala 210 215 220 Phe Arg Lys Asn Tyr Gln Asp Ala Gln Gly Phe Asp Asn Pro Pro Val 225 230 235 240 His Asp Pro Cys Ala Val Ala Tyr Leu Val Asp Pro Thr Val Phe Thr 245 250 255 Thr Arg Lys Ala Pro Leu Asp Val Glu Leu Tyr Gly Ala Leu Thr Thr 260 265 270 Gly Met Thr Val Ala Asp Phe Arg Ala Pro Ala Pro Ala Asp Cys Thr 275 280 285 Thr Gln Val Ala Val Asp Leu Asp Phe Asp Lys Phe Trp Asn Met Val 290 295 300 Ile Asp Ala Val Lys Arg Ile Gly 305 310 <210> 81 <211> 1078 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1048) <223> RXA02021 <400> 81 ttgggtcgcc gaggagatct aatcctggtt tgagttcaga gttcacaggt ttaagcctac 60 aaaccttagt taaaacatga tggaagcggt cgattaaaaa atg agt gaa aac att 115 Met Ser Glu Asn Ile 1 5 cgc gga gcc caa gca gtt gga atc gca aat atc gcc atg gac ggg acc 163 Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile Ala Met Asp Gly Thr 10 15 20 atc ctg gac acg tgg tac cca gaa ccc caa att ttc aac ccg gat cag 211 Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile Phe Asn Pro Asp Gln 25 30 35 tgg gct gaa cgc tac cca ttg gaa gtg ggc acc aca cgc ctc gga gca 259 Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr Thr Arg Leu Gly Ala 40 45 50 aac gaa ctc acc cca cgg atg ctg cag ttg gta aaa ctg gac caa gat 307 Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val Lys Leu Asp Gln Asp 55 60 65 cgc ctc gtc gaa cag gta gca gtc cgc acc gtt atc ccc gat ctg tct 355 Arg Leu Val Glu Gln Val Ala Val Arg Thr Val Ile Pro Asp Leu Ser 70 75 80 85 caa cct cca gta gac gcg cac gat gtt tac ctg cgc ctc cac ctg ctt 403 Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu Arg Leu His Leu Leu 90 95 100 tcc cac cgc ctg gtc cgc ccc cac gaa atg cac atg caa aac acc ttg 451 Ser His Arg Leu Val Arg Pro His Glu Met His Met Gln Asn Thr Leu 105 110 115 gag ctg ctg tcc gac gtg gtg tgg aca aac aag ggc cct tgc ctt cct 499 Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys Gly Pro Cys Leu Pro 120 125 130 gaa aac ttt gag tgg gtg cgt ggt gct ctg cgg tcc cgc gga ctc atc 547 Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg Ser Arg Gly Leu Ile 135 140 145 cac gtc tac tgt gtg gac cgt ctt ccc cgc atg gtc gac tat gtg gtt 595 His Val Tyr Cys Val Asp Arg Leu Pro Arg Met Val Asp Tyr Val Val 150 155 160 165 ccc cct gga gtc cgc atc tcc gaa gca gaa cgc gtg cgc cta ggt gca 643 Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg Val Arg Leu Gly Ala 170 175 180 tac ctt gct ccg ggt acc tct gtg ctg cgt gaa ggt ttc gtg tct ttc 691 Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu Gly Phe Val Ser Phe 185 190 195 aac tcc ggc acc ttg ggt gcc gca aag gtg gaa ggc cgc ctg agt tcc 739 Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu Gly Arg Leu Ser Ser 200 205 210 ggt gtg gtc atc ggt gaa ggt tcc gag att gga ctg tct tct act att 787 Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly Leu Ser Ser Thr Ile 215 220 225 cag tcc ccg aga gat gaa cag cgc cgc cgt ttg ccg ttg agc atc ggc 835 Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu Pro Leu Ser Ile Gly 230 235 240 245 caa aac tgc aac ttt ggt gtc agc tcc gga atc atc gga gtc agt ctg 883 Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile Ile Gly Val Ser Leu 250 255 260 gga gac aat tgc gac atc gga aat aac att gtc ttg gat gga gat acc 931 Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val Leu Asp Gly Asp Thr 265 270 275 ccc att tgg ttc gca gcc gat gag gag tta cgc act atc gac tcc atc 979 Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg Thr Ile Asp Ser Ile 280 285 290 gaa ggc caa gca aat tgg tca atc aag cgt gaa tcc ggc ttc cat gag 1027 Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu Ser Gly Phe His Glu 295 300 305 cca gtt gcc cgc ctc aaa gct tgacccattt tcataaccag tgccatgtgg 1078 Pro Val Ala Arg Leu Lys Ala 310 315 <210> 82 <211> 316 <212> PRT <213> Corynebacterium glutamicum <400> 82 Met Ser Glu Asn Ile Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile 1 5 10 15 Ala Met Asp Gly Thr Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile 20 25 30 Phe Asn Pro Asp Gln Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr 35 40 45 Thr Arg Leu Gly Ala Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val 50 55 60 Lys Leu Asp Gln Asp Arg Leu Val Glu Gln Val Ala Val Arg Thr Val 65 70 75 80 Ile Pro Asp Leu Ser Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu 85 90 95 Arg Leu His Leu Leu Ser His Arg Leu Val Arg Pro His Glu Met His 100 105 110 Met Gln Asn Thr Leu Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys 115 120 125 Gly Pro Cys Leu Pro Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg 130 135 140 Ser Arg Gly Leu Ile His Val Tyr Cys Val Asp Arg Leu Pro Arg Met 145 150 155 160 Val Asp Tyr Val Val Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg 165 170 175 Val Arg Leu Gly Ala Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu 180 185 190 Gly Phe Val Ser Phe Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu 195 200 205 Gly Arg Leu Ser Ser Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly 210 215 220 Leu Ser Ser Thr Ile Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu 225 230 235 240 Pro Leu Ser Ile Gly Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile 245 250 255 Ile Gly Val Ser Leu Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val 260 265 270 Leu Asp Gly Asp Thr Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg 275 280 285 Thr Ile Asp Ser Ile Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu 290 295 300 Ser Gly Phe His Glu Pro Val Ala Arg Leu Lys Ala 305 310 315 <210> 83 <211> 3871 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(3841) <223> RXA02095 <400> 83 ctctcttggt cctctcccca cccattttta agtactcaag acccttccaa cagaaaggat 60 tactccccca acaggctcaa aaatactgaa aggctcacgc atg aaa act gag caa 115 Met Lys Thr Glu Gln 1 5 tcc caa aaa gca caa tta gcc cct aag aaa gca cct gaa aag cca caa 163 Ser Gln Lys Ala Gln Leu Ala Pro Lys Lys Ala Pro Glu Lys Pro Gln 10 15 20 cgc atc cgc caa ctt att tcc gtg gcg tgg cag cga cct tgg ctc acc 211 Arg Ile Arg Gln Leu Ile Ser Val Ala Trp Gln Arg Pro Trp Leu Thr 25 30 35 tca ttc acc gta atc agc gct tta gct gca acg ttg ttt gaa ctt aca 259 Ser Phe Thr Val Ile Ser Ala Leu Ala Ala Thr Leu Phe Glu Leu Thr 40 45 50 ctt cct ctt ttg acc ggt ggc gcc atc gat atc gcg ctc gga aat acc 307 Leu Pro Leu Leu Thr Gly Gly Ala Ile Asp Ile Ala Leu Gly Asn Thr 55 60 65 gga gat act tta acc act gac ctg ctg gac cgg ttc act ccg agt gga 355 Gly Asp Thr Leu Thr Thr Asp Leu Leu Asp Arg Phe Thr Pro Ser Gly 70 75 80 85 tta agc gtg ttg acc agc gtc att gcc ctt atc gtg ctt ctc gcg ttg 403 Leu Ser Val Leu Thr Ser Val Ile Ala Leu Ile Val Leu Leu Ala Leu 90 95 100 ctt cgc tat gcc agt caa ttt gga cgg cga tac acc gca ggc aag ctc 451 Leu Arg Tyr Ala Ser Gln Phe Gly Arg Arg Tyr Thr Ala Gly Lys Leu 105 110 115 agc atg ggg gta cag cat gat gtc cgg ctt aaa acg atg cgc tca ttg 499 Ser Met Gly Val Gln His Asp Val Arg Leu Lys Thr Met Arg Ser Leu 120 125 130 cag aac ctc gat ggg cca ggt cag gac tct att cgc aca ggc caa gta 547 Gln Asn Leu Asp Gly Pro Gly Gln Asp Ser Ile Arg Thr Gly Gln Val 135 140 145 gtc agt cgg tcc att tcg gat atc aac atg gtg caa agc ctt gtg gcg 595 Val Ser Arg Ser Ile Ser Asp Ile Asn Met Val Gln Ser Leu Val Ala 150 155 160 165 atg ttg ccg atg ttg atc gga aat gtg gtc aag ctt gtg ctc act ttg 643 Met Leu Pro Met Leu Ile Gly Asn Val Val Lys Leu Val Leu Thr Leu 170 175 180 gtg atc atg ctg gct att tcc ccg ccg ctg acc atc atc gct gca gtg 691 Val Ile Met Leu Ala Ile Ser Pro Pro Leu Thr Ile Ile Ala Ala Val 185 190 195 ttg gtg cct ttg ctg ttg tgg gcc gtg gcc tat tcg cga aaa gcg ctt 739 Leu Val Pro Leu Leu Leu Trp Ala Val Ala Tyr Ser Arg Lys Ala Leu 200 205 210 ttt gcg tcc acg tgg tcg gcc cag caa aag gct gcg gat ctg acc act 787 Phe Ala Ser Thr Trp Ser Ala Gln Gln Lys Ala Ala Asp Leu Thr Thr 215 220 225 cat gtg gaa gaa act gtc acg ggt atc cgc gtg gtc aag gca ttt gcg 835 His Val Glu Glu Thr Val Thr Gly Ile Arg Val Val Lys Ala Phe Ala 230 235 240 245 cag gaa gac cgc gag acc gac aaa ttg gat ctc acc gca cgt gag tta 883 Gln Glu Asp Arg Glu Thr Asp Lys Leu Asp Leu Thr Ala Arg Glu Leu 250 255 260 ttt gcc cag cgc atg cgc act gca cgt ctg acg gca aag ttc atc ccc 931 Phe Ala Gln Arg Met Arg Thr Ala Arg Leu Thr Ala Lys Phe Ile Pro 265 270 275 atg gtt gag cag ctt ccg cag ctt gct ttg gtg gtc aac att gtt ggc 979 Met Val Glu Gln Leu Pro Gln Leu Ala Leu Val Val Asn Ile Val Gly 280 285 290 ggt ggc tat ttg gcc atg act ggt cac atc acg gtg ggc acg ttt gtg 1027 Gly Gly Tyr Leu Ala Met Thr Gly His Ile Thr Val Gly Thr Phe Val 295 300 305 gcg ttt tct tcc tat ctc act agc ttg tcg gcg gtg gct agg tcc ctg 1075 Ala Phe Ser Ser Tyr Leu Thr Ser Leu Ser Ala Val Ala Arg Ser Leu 310 315 320 325 tcg ggc atg ctc atg cgc gtg cag ttg gcg ctg tct tct gtg gag cgc 1123 Ser Gly Met Leu Met Arg Val Gln Leu Ala Leu Ser Ser Val Glu Arg 330 335 340 atc ttt gaa gtc att gat ctt cag cct gaa cgc acc gat cct gca cac 1171 Ile Phe Glu Val Ile Asp Leu Gln Pro Glu Arg Thr Asp Pro Ala His 345 350 355 ccc ctg tca ctt ccc gac act ccc ctg ggt ctg tcg ttc aac aac gta 1219 Pro Leu Ser Leu Pro Asp Thr Pro Leu Gly Leu Ser Phe Asn Asn Val 360 365 370 gat ttc cgt ggg att ctc aac ggt ttt gag ctg ggt gtt cag gcc ggt 1267 Asp Phe Arg Gly Ile Leu Asn Gly Phe Glu Leu Gly Val Gln Ala Gly 375 380 385 gaa acc gtt gtg ttg gtg ggc cct cca ggt tca ggc aag acc atg gct 1315 Glu Thr Val Val Leu Val Gly Pro Pro Gly Ser Gly Lys Thr Met Ala 390 395 400 405 gtg cag ctt gct gga aac ttt tat caa cca gac agc ggc cac atc gcc 1363 Val Gln Leu Ala Gly Asn Phe Tyr Gln Pro Asp Ser Gly His Ile Ala 410 415 420 ttt gat agc aac ggc cat cgc act cgc ttc gac gac ctc acc cac agc 1411 Phe Asp Ser Asn Gly His Arg Thr Arg Phe Asp Asp Leu Thr His Ser 425 430 435 gat atc cgc agg aat ctc atc gcg gtt ttt gat gag ccg ttc ttg tac 1459 Asp Ile Arg Arg Asn Leu Ile Ala Val Phe Asp Glu Pro Phe Leu Tyr 440 445 450 tcc tcc tcc atc cgc gag aac atc tcg atg ggt ttg gat gtc agt gat 1507 Ser Ser Ser Ile Arg Glu Asn Ile Ser Met Gly Leu Asp Val Ser Asp 455 460 465 gag cag atc gaa cac gca gcc agg ctt gcc cag gct cat gat ttt atc 1555 Glu Gln Ile Glu His Ala Ala Arg Leu Ala Gln Ala His Asp Phe Ile 470 475 480 485 gat cgc ctt cca aac aaa tac gag gaa gtc att ggc gaa cgc ggc ctg 1603 Asp Arg Leu Pro Asn Lys Tyr Glu Glu Val Ile Gly Glu Arg Gly Leu 490 495 500 acg ctt tct ggt ggt caa cgc caa cgc atc gcc ctc gca cgg gct ttc 1651 Thr Leu Ser Gly Gly Gln Arg Gln Arg Ile Ala Leu Ala Arg Ala Phe 505 510 515 ctg gcg cat ccc aaa gtg ttg gtg ctt gat gat gcc acc tct gcc att 1699 Leu Ala His Pro Lys Val Leu Val Leu Asp Asp Ala Thr Ser Ala Ile 520 525 530 gat gcc tcc act gag gac cgc att ttc cag gcc ttg cgc gaa gaa ctg 1747 Asp Ala Ser Thr Glu Asp Arg Ile Phe Gln Ala Leu Arg Glu Glu Leu 535 540 545 cac gat gtc acc att ttg atc atc gcg cac cgc cac tcc act ttg gag 1795 His Asp Val Thr Ile Leu Ile Ile Ala His Arg His Ser Thr Leu Glu 550 555 560 565 ctc ggc gat cgg gtt ggt ctg gtc gaa gat gga cgg gta aca gca ctg 1843 Leu Gly Asp Arg Val Gly Leu Val Glu Asp Gly Arg Val Thr Ala Leu 570 575 580 gga ccg ttg agt gag atg cgt gat cac gct cgt ttc tcg cat ctg atg 1891 Gly Pro Leu Ser Glu Met Arg Asp His Ala Arg Phe Ser His Leu Met 585 590 595 gct ctt gat ttc cag gat tct cac gat ccg gaa ttc acc ctc gac aac 1939 Ala Leu Asp Phe Gln Asp Ser His Asp Pro Glu Phe Thr Leu Asp Asn 600 605 610 ggt tca cta ccc agc caa gag caa ttg tgg ccg gag gtc tcc aca gaa 1987 Gly Ser Leu Pro Ser Gln Glu Gln Leu Trp Pro Glu Val Ser Thr Glu 615 620 625 aag cag tac aag att ctt gcg cct gcc cct ggt cga ggc cgt ggc atg 2035 Lys Gln Tyr Lys Ile Leu Ala Pro Ala Pro Gly Arg Gly Arg Gly Met 630 635 640 645 tcc atg cca gca acc cct gag ctg ctc gcc cag att gag gcg ctg cca 2083 Ser Met Pro Ala Thr Pro Glu Leu Leu Ala Gln Ile Glu Ala Leu Pro 650 655 660 gca gca acg gaa gaa aca cga gtt gat gcc ggg agg cta cgc acc agt 2131 Ala Ala Thr Glu Glu Thr Arg Val Asp Ala Gly Arg Leu Arg Thr Ser 665 670 675 acc tcc ggt ttc aaa ttg ctc agt tta ttc aag cag gtc cgt tgg ctc 2179 Thr Ser Gly Phe Lys Leu Leu Ser Leu Phe Lys Gln Val Arg Trp Leu 680 685 690 gtc gtc gcg gtc atc gcg ttg ttg ctg gtg ggc gta gcc gcc gat cta 2227 Val Val Ala Val Ile Ala Leu Leu Leu Val Gly Val Ala Ala Asp Leu 695 700 705 gca ttt cca aca ctg atg cgc gca gcc atc gac aac ggt gtg caa gca 2275 Ala Phe Pro Thr Leu Met Arg Ala Ala Ile Asp Asn Gly Val Gln Ala 710 715 720 725 caa agc acc tcc acg ttg tgg tgg atc gcc atc gca ggc agc gta gta 2323 Gln Ser Thr Ser Thr Leu Trp Trp Ile Ala Ile Ala Gly Ser Val Val 730 735 740 gtc ctt ctg tcc tgg gcc gcc gcc gcg atc aac acg att atc acg gca 2371 Val Leu Leu Ser Trp Ala Ala Ala Ala Ile Asn Thr Ile Ile Thr Ala 745 750 755 cgc acc ggt gaa cgg ctg ctt tac ggc ttg cgt ctg cgc tca ttt gtg 2419 Arg Thr Gly Glu Arg Leu Leu Tyr Gly Leu Arg Leu Arg Ser Phe Val 760 765 770 cat cta ttg cgc ctg tcc atg agc tat ttc gaa cgc acc atg tcc ggc 2467 His Leu Leu Arg Leu Ser Met Ser Tyr Phe Glu Arg Thr Met Ser Gly 775 780 785 cgc atc atg acg cgc atg acc acc gac atc gac aac ctc tcg tcc ttc 2515 Arg Ile Met Thr Arg Met Thr Thr Asp Ile Asp Asn Leu Ser Ser Phe 790 795 800 805 ctc caa tca ggt ctg gcg caa aca gtt gtc tct gtg ggc acg ctc atc 2563 Leu Gln Ser Gly Leu Ala Gln Thr Val Val Ser Val Gly Thr Leu Ile 810 815 820 ggt gtg gtc acc atg ctc gcc atc acc gac gca caa cta gca ctc gtt 2611 Gly Val Val Thr Met Leu Ala Ile Thr Asp Ala Gln Leu Ala Leu Val 825 830 835 gcg ctg tcc gtg gtg ccg atc atc atc gtg ctc act ctc att ttc cga 2659 Ala Leu Ser Val Val Pro Ile Ile Ile Val Leu Thr Leu Ile Phe Arg 840 845 850 cgc atc agc tcc agg ctg tac acc gct tca cgc gag caa gcc agc cag 2707 Arg Ile Ser Ser Arg Leu Tyr Thr Ala Ser Arg Glu Gln Ala Ser Gln 855 860 865 gtc aac gcg gta ttc cac gag tcc atc gcc ggt tta cgc acc gcg cag 2755 Val Asn Ala Val Phe His Glu Ser Ile Ala Gly Leu Arg Thr Ala Gln 870 875 880 885 atg cac cgc atg gaa gac caa gtc ttt gac aat tat gcg ggc gaa gca 2803 Met His Arg Met Glu Asp Gln Val Phe Asp Asn Tyr Ala Gly Glu Ala 890 895 900 gag gaa ttc cga cgc ctg cgt gtg aaa tcc cag acg gcc atc gcc atc 2851 Glu Glu Phe Arg Arg Leu Arg Val Lys Ser Gln Thr Ala Ile Ala Ile 905 910 915 tac ttc ccc ggc ctt ggc gcg ctc tct gaa atc gcc cag gca ctc gtc 2899 Tyr Phe Pro Gly Leu Gly Ala Leu Ser Glu Ile Ala Gln Ala Leu Val 920 925 930 ctc ggt ttc ggc gca ctg caa gta acg cgc ggc gac atc tcc acc ggc 2947 Leu Gly Phe Gly Ala Leu Gln Val Thr Arg Gly Asp Ile Ser Thr Gly 935 940 945 gta ctc gtg gca ttc gtg ctg tac atg ggc ctg atg ttc ggc ccc atc 2995 Val Leu Val Ala Phe Val Leu Tyr Met Gly Leu Met Phe Gly Pro Ile 950 955 960 965 caa caa cta agc caa atc ttc gac tcc tac caa caa gcc gcc gtc ggc 3043 Gln Gln Leu Ser Gln Ile Phe Asp Ser Tyr Gln Gln Ala Ala Val Gly 970 975 980 ttc cgt cgc atc acc gaa ctg ctc gca acg cag ccc agc gtc cca gat 3091 Phe Arg Arg Ile Thr Glu Leu Leu Ala Thr Gln Pro Ser Val Pro Asp 985 990 995 ctg ggc acc aac agg cac gct agg cag gct gcc acg cag cct tta ttg 3139 Leu Gly Thr Asn Arg His Ala Arg Gln Ala Ala Thr Gln Pro Leu Leu 1000 1005 1010 ctt gac gac gtc acc ttc ggc tat tca gac gat ccg atc cta gac aac 3187 Leu Asp Asp Val Thr Phe Gly Tyr Ser Asp Asp Pro Ile Leu Asp Asn 1015 1020 1025 gtc acc gtc cag atc gaa ccg gga acc acc gtc gct gtg gtg gga cca 3235 Val Thr Val Gln Ile Glu Pro Gly Thr Thr Val Ala Val Val Gly Pro 1030 1035 1040 1045 acc ggc gct gga aaa tcc acc gtg gtg aaa ctc ctt tcc cgc ctc tac 3283 Thr Gly Ala Gly Lys Ser Thr Val Val Lys Leu Leu Ser Arg Leu Tyr 1050 1055 1060 gac ccc aat gaa ggt gcc gtg aaa gct ggc gag atc gat atc aag gac 3331 Asp Pro Asn Glu Gly Ala Val Lys Ala Gly Glu Ile Asp Ile Lys Asp 1065 1070 1075 ttc ccc acc gct gat tgg cgc cgc aca atc ggt acc gtg cca caa gaa 3379 Phe Pro Thr Ala Asp Trp Arg Arg Thr Ile Gly Thr Val Pro Gln Glu 1080 1085 1090 gca cac ttg ttt agt gga agc atc gcc gac aac att ggc tac gga tgc 3427 Ala His Leu Phe Ser Gly Ser Ile Ala Asp Asn Ile Gly Tyr Gly Cys 1095 1100 1105 agg gag gcg tcg aca agc aaa atc gaa gcg gca gca cgc cgc gtc gga 3475 Arg Glu Ala Ser Thr Ser Lys Ile Glu Ala Ala Ala Arg Arg Val Gly 1110 1115 1120 1125 gcc tta aac gcc atc gcc gcc atc cct gat ggt ttc aac cat caa gtc 3523 Ala Leu Asn Ala Ile Ala Ala Ile Pro Asp Gly Phe Asn His Gln Val 1130 1135 1140 ggt gaa cgc ggg cgc aac ctg tca tcc gga cag cgc caa ctg atc gcg 3571 Gly Glu Arg Gly Arg Asn Leu Ser Ser Gly Gln Arg Gln Leu Ile Ala 1145 1150 1155 ctg gcg cgc gcc gaa ctt atc gag cct tcc atc atg ctt ctc gac gaa 3619 Leu Ala Arg Ala Glu Leu Ile Glu Pro Ser Ile Met Leu Leu Asp Glu 1160 1165 1170 gcc acc tcc acc ctc gac ccc gcc acc gaa gcc gtt atc ctc aac gcc 3667 Ala Thr Ser Thr Leu Asp Pro Ala Thr Glu Ala Val Ile Leu Asn Ala 1175 1180 1185 tcc gat cga gtc act aag gga cgc acc agc atc atc gtc gcg cac cgc 3715 Ser Asp Arg Val Thr Lys Gly Arg Thr Ser Ile Ile Val Ala His Arg 1190 1195 1200 1205 ttg gca acc gct aaa agg gcc gac cgt att ctt gtt gtt gaa caa gga 3763 Leu Ala Thr Ala Lys Arg Ala Asp Arg Ile Leu Val Val Glu Gln Gly 1210 1215 1220 cgt atc att gag gac gga tct cac gac gcg ttg ttg tct gct aac ggc 3811 Arg Ile Ile Glu Asp Gly Ser His Asp Ala Leu Leu Ser Ala Asn Gly 1225 1230 1235 acc tac gcc cgc atg tgg cat tta atg gcc tgacacgtta tttttaggag 3861 Thr Tyr Ala Arg Met Trp His Leu Met Ala 1240 1245 aactgtcaac 3871 <210> 84 <211> 1247 <212> PRT <213> Corynebacterium glutamicum <400> 84 Met Lys Thr Glu Gln Ser Gln Lys Ala Gln Leu Ala Pro Lys Lys Ala 1 5 10 15 Pro Glu Lys Pro Gln Arg Ile Arg Gln Leu Ile Ser Val Ala Trp Gln 20 25 30 Arg Pro Trp Leu Thr Ser Phe Thr Val Ile Ser Ala Leu Ala Ala Thr 35 40 45 Leu Phe Glu Leu Thr Leu Pro Leu Leu Thr Gly Gly Ala Ile Asp Ile 50 55 60 Ala Leu Gly Asn Thr Gly Asp Thr Leu Thr Thr Asp Leu Leu Asp Arg 65 70 75 80 Phe Thr Pro Ser Gly Leu Ser Val Leu Thr Ser Val Ile Ala Leu Ile 85 90 95 Val Leu Leu Ala Leu Leu Arg Tyr Ala Ser Gln Phe Gly Arg Arg Tyr 100 105 110 Thr Ala Gly Lys Leu Ser Met Gly Val Gln His Asp Val Arg Leu Lys 115 120 125 Thr Met Arg Ser Leu Gln Asn Leu Asp Gly Pro Gly Gln Asp Ser Ile 130 135 140 Arg Thr Gly Gln Val Val Ser Arg Ser Ile Ser Asp Ile Asn Met Val 145 150 155 160 Gln Ser Leu Val Ala Met Leu Pro Met Leu Ile Gly Asn Val Val Lys 165 170 175 Leu Val Leu Thr Leu Val Ile Met Leu Ala Ile Ser Pro Pro Leu Thr 180 185 190 Ile Ile Ala Ala Val Leu Val Pro Leu Leu Leu Trp Ala Val Ala Tyr 195 200 205 Ser Arg Lys Ala Leu Phe Ala Ser Thr Trp Ser Ala Gln Gln Lys Ala 210 215 220 Ala Asp Leu Thr Thr His Val Glu Glu Thr Val Thr Gly Ile Arg Val 225 230 235 240 Val Lys Ala Phe Ala Gln Glu Asp Arg Glu Thr Asp Lys Leu Asp Leu 245 250 255 Thr Ala Arg Glu Leu Phe Ala Gln Arg Met Arg Thr Ala Arg Leu Thr 260 265 270 Ala Lys Phe Ile Pro Met Val Glu Gln Leu Pro Gln Leu Ala Leu Val 275 280 285 Val Asn Ile Val Gly Gly Gly Tyr Leu Ala Met Thr Gly His Ile Thr 290 295 300 Val Gly Thr Phe Val Ala Phe Ser Ser Tyr Leu Thr Ser Leu Ser Ala 305 310 315 320 Val Ala Arg Ser Leu Ser Gly Met Leu Met Arg Val Gln Leu Ala Leu 325 330 335 Ser Ser Val Glu Arg Ile Phe Glu Val Ile Asp Leu Gln Pro Glu Arg 340 345 350 Thr Asp Pro Ala His Pro Leu Ser Leu Pro Asp Thr Pro Leu Gly Leu 355 360 365 Ser Phe Asn Asn Val Asp Phe Arg Gly Ile Leu Asn Gly Phe Glu Leu 370 375 380 Gly Val Gln Ala Gly Glu Thr Val Val Leu Val Gly Pro Pro Gly Ser 385 390 395 400 Gly Lys Thr Met Ala Val Gln Leu Ala Gly Asn Phe Tyr Gln Pro Asp 405 410 415 Ser Gly His Ile Ala Phe Asp Ser Asn Gly His Arg Thr Arg Phe Asp 420 425 430 Asp Leu Thr His Ser Asp Ile Arg Arg Asn Leu Ile Ala Val Phe Asp 435 440 445 Glu Pro Phe Leu Tyr Ser Ser Ser Ile Arg Glu Asn Ile Ser Met Gly 450 455 460 Leu Asp Val Ser Asp Glu Gln Ile Glu His Ala Ala Arg Leu Ala Gln 465 470 475 480 Ala His Asp Phe Ile Asp Arg Leu Pro Asn Lys Tyr Glu Glu Val Ile 485 490 495 Gly Glu Arg Gly Leu Thr Leu Ser Gly Gly Gln Arg Gln Arg Ile Ala 500 505 510 Leu Ala Arg Ala Phe Leu Ala His Pro Lys Val Leu Val Leu Asp Asp 515 520 525 Ala Thr Ser Ala Ile Asp Ala Ser Thr Glu Asp Arg Ile Phe Gln Ala 530 535 540 Leu Arg Glu Glu Leu His Asp Val Thr Ile Leu Ile Ile Ala His Arg 545 550 555 560 His Ser Thr Leu Glu Leu Gly Asp Arg Val Gly Leu Val Glu Asp Gly 565 570 575 Arg Val Thr Ala Leu Gly Pro Leu Ser Glu Met Arg Asp His Ala Arg 580 585 590 Phe Ser His Leu Met Ala Leu Asp Phe Gln Asp Ser His Asp Pro Glu 595 600 605 Phe Thr Leu Asp Asn Gly Ser Leu Pro Ser Gln Glu Gln Leu Trp Pro 610 615 620 Glu Val Ser Thr Glu Lys Gln Tyr Lys Ile Leu Ala Pro Ala Pro Gly 625 630 635 640 Arg Gly Arg Gly Met Ser Met Pro Ala Thr Pro Glu Leu Leu Ala Gln 645 650 655 Ile Glu Ala Leu Pro Ala Ala Thr Glu Glu Thr Arg Val Asp Ala Gly 660 665 670 Arg Leu Arg Thr Ser Thr Ser Gly Phe Lys Leu Leu Ser Leu Phe Lys 675 680 685 Gln Val Arg Trp Leu Val Val Ala Val Ile Ala Leu Leu Leu Val Gly 690 695 700 Val Ala Ala Asp Leu Ala Phe Pro Thr Leu Met Arg Ala Ala Ile Asp 705 710 715 720 Asn Gly Val Gln Ala Gln Ser Thr Ser Thr Leu Trp Trp Ile Ala Ile 725 730 735 Ala Gly Ser Val Val Val Leu Leu Ser Trp Ala Ala Ala Ala Ile Asn 740 745 750 Thr Ile Ile Thr Ala Arg Thr Gly Glu Arg Leu Leu Tyr Gly Leu Arg 755 760 765 Leu Arg Ser Phe Val His Leu Leu Arg Leu Ser Met Ser Tyr Phe Glu 770 775 780 Arg Thr Met Ser Gly Arg Ile Met Thr Arg Met Thr Thr Asp Ile Asp 785 790 795 800 Asn Leu Ser Ser Phe Leu Gln Ser Gly Leu Ala Gln Thr Val Val Ser 805 810 815 Val Gly Thr Leu Ile Gly Val Val Thr Met Leu Ala Ile Thr Asp Ala 820 825 830 Gln Leu Ala Leu Val Ala Leu Ser Val Val Pro Ile Ile Ile Val Leu 835 840 845 Thr Leu Ile Phe Arg Arg Ile Ser Ser Arg Leu Tyr Thr Ala Ser Arg 850 855 860 Glu Gln Ala Ser Gln Val Asn Ala Val Phe His Glu Ser Ile Ala Gly 865 870 875 880 Leu Arg Thr Ala Gln Met His Arg Met Glu Asp Gln Val Phe Asp Asn 885 890 895 Tyr Ala Gly Glu Ala Glu Glu Phe Arg Arg Leu Arg Val Lys Ser Gln 900 905 910 Thr Ala Ile Ala Ile Tyr Phe Pro Gly Leu Gly Ala Leu Ser Glu Ile 915 920 925 Ala Gln Ala Leu Val Leu Gly Phe Gly Ala Leu Gln Val Thr Arg Gly 930 935 940 Asp Ile Ser Thr Gly Val Leu Val Ala Phe Val Leu Tyr Met Gly Leu 945 950 955 960 Met Phe Gly Pro Ile Gln Gln Leu Ser Gln Ile Phe Asp Ser Tyr Gln 965 970 975 Gln Ala Ala Val Gly Phe Arg Arg Ile Thr Glu Leu Leu Ala Thr Gln 980 985 990 Pro Ser Val Pro Asp Leu Gly Thr Asn Arg His Ala Arg Gln Ala Ala 995 1000 1005 Thr Gln Pro Leu Leu Leu Asp Asp Val Thr Phe Gly Tyr Ser Asp Asp 1010 1015 1020 Pro Ile Leu Asp Asn Val Thr Val Gln Ile Glu Pro Gly Thr Thr Val 1025 1030 1035 1040 Ala Val Val Gly Pro Thr Gly Ala Gly Lys Ser Thr Val Val Lys Leu 1045 1050 1055 Leu Ser Arg Leu Tyr Asp Pro Asn Glu Gly Ala Val Lys Ala Gly Glu 1060 1065 1070 Ile Asp Ile Lys Asp Phe Pro Thr Ala Asp Trp Arg Arg Thr Ile Gly 1075 1080 1085 Thr Val Pro Gln Glu Ala His Leu Phe Ser Gly Ser Ile Ala Asp Asn 1090 1095 1100 Ile Gly Tyr Gly Cys Arg Glu Ala Ser Thr Ser Lys Ile Glu Ala Ala 1105 1110 1115 1120 Ala Arg Arg Val Gly Ala Leu Asn Ala Ile Ala Ala Ile Pro Asp Gly 1125 1130 1135 Phe Asn His Gln Val Gly Glu Arg Gly Arg Asn Leu Ser Ser Gly Gln 1140 1145 1150 Arg Gln Leu Ile Ala Leu Ala Arg Ala Glu Leu Ile Glu Pro Ser Ile 1155 1160 1165 Met Leu Leu Asp Glu Ala Thr Ser Thr Leu Asp Pro Ala Thr Glu Ala 1170 1175 1180 Val Ile Leu Asn Ala Ser Asp Arg Val Thr Lys Gly Arg Thr Ser Ile 1185 1190 1195 1200 Ile Val Ala His Arg Leu Ala Thr Ala Lys Arg Ala Asp Arg Ile Leu 1205 1210 1215 Val Val Glu Gln Gly Arg Ile Ile Glu Asp Gly Ser His Asp Ala Leu 1220 1225 1230 Leu Ser Ala Asn Gly Thr Tyr Ala Arg Met Trp His Leu Met Ala 1235 1240 1245 <210> 85 <211> 1081 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1051) <223> RXA02156 <400> 85 aaccactgac ctgagcttct cctacgtgga gatcaactcc gcgtacagct cttaaaaaga 60 aacagcactc caactaacaa gcagggaaaa gggcacaggc atg aat gac ttg atc 115 Met Asn Asp Leu Ile 1 5 aaa gat tta ggc tct gag gtg cgc gca aat gtc ctc gct gag gcg ttg 163 Lys Asp Leu Gly Ser Glu Val Arg Ala Asn Val Leu Ala Glu Ala Leu 10 15 20 cca tgg ttg cag cac ttc cgc gac aag att gtt gtc gtg aaa tat ggc 211 Pro Trp Leu Gln His Phe Arg Asp Lys Ile Val Val Val Lys Tyr Gly 25 30 35 gga aac gcc atg gtg gat gat gat ctc aag gct gct ttt gct gcc gac 259 Gly Asn Ala Met Val Asp Asp Asp Leu Lys Ala Ala Phe Ala Ala Asp 40 45 50 atg gtc ttc ttg cgc acc gtg ggc gca aaa cca gtg gtg gtg cac ggt 307 Met Val Phe Leu Arg Thr Val Gly Ala Lys Pro Val Val Val His Gly 55 60 65 ggt gga cct cag att tct gag atg cta aac cgt gtg ggt ctc cag ggc 355 Gly Gly Pro Gln Ile Ser Glu Met Leu Asn Arg Val Gly Leu Gln Gly 70 75 80 85 gag ttc aag ggt ggt ttc cgt gtg acc act cct gag gtc atg gac att 403 Glu Phe Lys Gly Gly Phe Arg Val Thr Thr Pro Glu Val Met Asp Ile 90 95 100 gtg cgc atg gtg ctc ttt ggt cag gtc ggt cgc gat tta gtt ggt ttg 451 Val Arg Met Val Leu Phe Gly Gln Val Gly Arg Asp Leu Val Gly Leu 105 110 115 atc aac tct cat ggc cct tac gct gtg gga acc tcc ggt gag gat gcc 499 Ile Asn Ser His Gly Pro Tyr Ala Val Gly Thr Ser Gly Glu Asp Ala 120 125 130 ggc ctg ttt acc gcg cag aag cgc atg gtc aac atc gat ggc gta ccc 547 Gly Leu Phe Thr Ala Gln Lys Arg Met Val Asn Ile Asp Gly Val Pro 135 140 145 act gat att ggt ttg gtc gga gac atc att aat gtc gat gcc tct tcc 595 Thr Asp Ile Gly Leu Val Gly Asp Ile Ile Asn Val Asp Ala Ser Ser 150 155 160 165 ttg atg gat atc atc gag gcc ggt cgc att cct gtg gtc tct acg att 643 Leu Met Asp Ile Ile Glu Ala Gly Arg Ile Pro Val Val Ser Thr Ile 170 175 180 gct cca ggc gaa gac ggc cag att tac aac att aac gcc gat acc gca 691 Ala Pro Gly Glu Asp Gly Gln Ile Tyr Asn Ile Asn Ala Asp Thr Ala 185 190 195 gca ggt gct ttg gct gca gcg att ggt gca gaa cgc ctg ctg gtt ctc 739 Ala Gly Ala Leu Ala Ala Ala Ile Gly Ala Glu Arg Leu Leu Val Leu 200 205 210 acc aat gtg gaa ggt ctg tac acc gat tgg cct gat aag agc tca ctg 787 Thr Asn Val Glu Gly Leu Tyr Thr Asp Trp Pro Asp Lys Ser Ser Leu 215 220 225 gtg tcc aag atc aag gcc acc gag ctg gag gcc att ctt ccg gga ctt 835 Val Ser Lys Ile Lys Ala Thr Glu Leu Glu Ala Ile Leu Pro Gly Leu 230 235 240 245 gat tcc ggc atg att cca aag atg gag tct tgc ttg aac gcg gtg cgt 883 Asp Ser Gly Met Ile Pro Lys Met Glu Ser Cys Leu Asn Ala Val Arg 250 255 260 ggg gga gta agc gct gct cat gtc att gac ggc cgc atc gcg cac tcg 931 Gly Gly Val Ser Ala Ala His Val Ile Asp Gly Arg Ile Ala His Ser 265 270 275 gtg ttg ctg gag ctt ttg acc atg ggt gga att ggc acg atg gtg ctg 979 Val Leu Leu Glu Leu Leu Thr Met Gly Gly Ile Gly Thr Met Val Leu 280 285 290 ccg gat gtt ttt gat cgg gag aat tat cct gaa ggc acc gtt ttt aga 1027 Pro Asp Val Phe Asp Arg Glu Asn Tyr Pro Glu Gly Thr Val Phe Arg 295 300 305 aaa gac gac aag gat ggg gaa ctg taaatgagca cgctggaaac ttggccacag 1081 Lys Asp Asp Lys Asp Gly Glu Leu 310 315 <210> 86 <211> 317 <212> PRT <213> Corynebacterium glutamicum <400> 86 Met Asn Asp Leu Ile Lys Asp Leu Gly Ser Glu Val Arg Ala Asn Val 1 5 10 15 Leu Ala Glu Ala Leu Pro Trp Leu Gln His Phe Arg Asp Lys Ile Val 20 25 30 Val Val Lys Tyr Gly Gly Asn Ala Met Val Asp Asp Asp Leu Lys Ala 35 40 45 Ala Phe Ala Ala Asp Met Val Phe Leu Arg Thr Val Gly Ala Lys Pro 50 55 60 Val Val Val His Gly Gly Gly Pro Gln Ile Ser Glu Met Leu Asn Arg 65 70 75 80 Val Gly Leu Gln Gly Glu Phe Lys Gly Gly Phe Arg Val Thr Thr Pro 85 90 95 Glu Val Met Asp Ile Val Arg Met Val Leu Phe Gly Gln Val Gly Arg 100 105 110 Asp Leu Val Gly Leu Ile Asn Ser His Gly Pro Tyr Ala Val Gly Thr 115 120 125 Ser Gly Glu Asp Ala Gly Leu Phe Thr Ala Gln Lys Arg Met Val Asn 130 135 140 Ile Asp Gly Val Pro Thr Asp Ile Gly Leu Val Gly Asp Ile Ile Asn 145 150 155 160 Val Asp Ala Ser Ser Leu Met Asp Ile Ile Glu Ala Gly Arg Ile Pro 165 170 175 Val Val Ser Thr Ile Ala Pro Gly Glu Asp Gly Gln Ile Tyr Asn Ile 180 185 190 Asn Ala Asp Thr Ala Ala Gly Ala Leu Ala Ala Ala Ile Gly Ala Glu 195 200 205 Arg Leu Leu Val Leu Thr Asn Val Glu Gly Leu Tyr Thr Asp Trp Pro 210 215 220 Asp Lys Ser Ser Leu Val Ser Lys Ile Lys Ala Thr Glu Leu Glu Ala 225 230 235 240 Ile Leu Pro Gly Leu Asp Ser Gly Met Ile Pro Lys Met Glu Ser Cys 245 250 255 Leu Asn Ala Val Arg Gly Gly Val Ser Ala Ala His Val Ile Asp Gly 260 265 270 Arg Ile Ala His Ser Val Leu Leu Glu Leu Leu Thr Met Gly Gly Ile 275 280 285 Gly Thr Met Val Leu Pro Asp Val Phe Asp Arg Glu Asn Tyr Pro Glu 290 295 300 Gly Thr Val Phe Arg Lys Asp Asp Lys Asp Gly Glu Leu 305 310 315 <210> 87 <211> 1561 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1531) <223> RXA02162 <400> 87 gatcgctaac aagcgcgatc gcgaagctgg caacaactaa gccacctttt caagcatcca 60 gactagaact tcaagtattt agaaagtaga agaacaccac atg gaa cag cac gga 115 Met Glu Gln His Gly 1 5 acc aat gaa ggt gcg ctg tgg ggc ggc cgc ttc tcc ggt gga ccc tcc 163 Thr Asn Glu Gly Ala Leu Trp Gly Gly Arg Phe Ser Gly Gly Pro Ser 10 15 20 gag gcc atg ttc gcc ttg agt gtc tcc act cat ttc gac tgg gtt ttg 211 Glu Ala Met Phe Ala Leu Ser Val Ser Thr His Phe Asp Trp Val Leu 25 30 35 gcc cct tat gat gtg ttg gcc tcc aag gca cac gcc aag gtt ttg cac 259 Ala Pro Tyr Asp Val Leu Ala Ser Lys Ala His Ala Lys Val Leu His 40 45 50 caa gca gat cta ctt tct gat gaa gat cta gcc acc atg ctg gct ggg 307 Gln Ala Asp Leu Leu Ser Asp Glu Asp Leu Ala Thr Met Leu Ala Gly 55 60 65 ctt gat cag ctg ggc aag gat gtc gcc gac gga acc ttc ggt ccg ctg 355 Leu Asp Gln Leu Gly Lys Asp Val Ala Asp Gly Thr Phe Gly Pro Leu 70 75 80 85 cct tct gat gag gat gtg cac ggc gcg atg gaa cgc ggt ctg att gac 403 Pro Ser Asp Glu Asp Val His Gly Ala Met Glu Arg Gly Leu Ile Asp 90 95 100 cgc gtt ggt cct gag gtg ggc ggc cgt ctg cgc gct ggt cgt tcc cgc 451 Arg Val Gly Pro Glu Val Gly Gly Arg Leu Arg Ala Gly Arg Ser Arg 105 110 115 aac gac cag gtg gca acc ctg ttc cgc atg tgg gtc cgc gac gca gtg 499 Asn Asp Gln Val Ala Thr Leu Phe Arg Met Trp Val Arg Asp Ala Val 120 125 130 cgc gac atc gcg ctg gga aca acc gag ctt gtc gac gcc ctc agc gcc 547 Arg Asp Ile Ala Leu Gly Thr Thr Glu Leu Val Asp Ala Leu Ser Ala 135 140 145 caa gct aag gca cat gca ggc gcg atc atg cca ggc aag acc cac ttc 595 Gln Ala Lys Ala His Ala Gly Ala Ile Met Pro Gly Lys Thr His Phe 150 155 160 165 cag gca gct cag ccg gtc ctt ctg gca cac cag ctg ctg gca cac gca 643 Gln Ala Ala Gln Pro Val Leu Leu Ala His Gln Leu Leu Ala His Ala 170 175 180 cag cct ttg ctg cgc gat att gat cgt atc cgt gac ctg gac aag cgt 691 Gln Pro Leu Leu Arg Asp Ile Asp Arg Ile Arg Asp Leu Asp Lys Arg 185 190 195 ctt gcg gtg tct cct tac ggt tcc ggc gca ctt gct ggt tcc tct ttg 739 Leu Ala Val Ser Pro Tyr Gly Ser Gly Ala Leu Ala Gly Ser Ser Leu 200 205 210 aag ctc aac cct gaa gca atc gct gaa gaa ctc ggc ttt gat tcc gca 787 Lys Leu Asn Pro Glu Ala Ile Ala Glu Glu Leu Gly Phe Asp Ser Ala 215 220 225 gca gat aac tcc att gat gcc acc agc tcc cgc gat ttc gca tct gaa 835 Ala Asp Asn Ser Ile Asp Ala Thr Ser Ser Arg Asp Phe Ala Ser Glu 230 235 240 245 acc gcc ttc gtg ctg gcg cag ctt gca gtg gat atg tcc cgc ttg gct 883 Thr Ala Phe Val Leu Ala Gln Leu Ala Val Asp Met Ser Arg Leu Ala 250 255 260 gaa gaa atc atc gca tgg tgc acc cca gaa ttt ggt tac atc acc ttg 931 Glu Glu Ile Ile Ala Trp Cys Thr Pro Glu Phe Gly Tyr Ile Thr Leu 265 270 275 tct gat tcc tgg tcc aca ggc agc tca atc atg ccg cag aag aag aac 979 Ser Asp Ser Trp Ser Thr Gly Ser Ser Ile Met Pro Gln Lys Lys Asn 280 285 290 cct gac gtg gca gag ctg acc cgt ggc aag tct ggt cgc ttg atc ggt 1027 Pro Asp Val Ala Glu Leu Thr Arg Gly Lys Ser Gly Arg Leu Ile Gly 295 300 305 aac ctc acc ggt ctg ctg gct acc ctg aag gca cag cct tta gcg tac 1075 Asn Leu Thr Gly Leu Leu Ala Thr Leu Lys Ala Gln Pro Leu Ala Tyr 310 315 320 325 aac cgc gac ctg cag gaa gat aag gaa cca atc gta gat tcc gtg gcg 1123 Asn Arg Asp Leu Gln Glu Asp Lys Glu Pro Ile Val Asp Ser Val Ala 330 335 340 cag ctc aac ctg ctg ctc cct gca atg act ggt ttg gtt tcc acc ttg 1171 Gln Leu Asn Leu Leu Leu Pro Ala Met Thr Gly Leu Val Ser Thr Leu 345 350 355 acc ttc aac acc gag cgc atg cgt gaa ctt gca cca gca ggt ttc acc 1219 Thr Phe Asn Thr Glu Arg Met Arg Glu Leu Ala Pro Ala Gly Phe Thr 360 365 370 ctt gcc acc gac ttg gct gag tgg atg gtg cgc cag ggc gtt cca ttc 1267 Leu Ala Thr Asp Leu Ala Glu Trp Met Val Arg Gln Gly Val Pro Phe 375 380 385 cgt gag gca cac gaa gca tcc ggc gct tgc gtg cgg atc gcg gag tcc 1315 Arg Glu Ala His Glu Ala Ser Gly Ala Cys Val Arg Ile Ala Glu Ser 390 395 400 405 agg gga gtg gac ctt atc gat ctc act gat gaa gaa ctc agt ggc gtt 1363 Arg Gly Val Asp Leu Ile Asp Leu Thr Asp Glu Glu Leu Ser Gly Val 410 415 420 gat gca cgt ctg acc cca gag gta cgg gaa gtg ctc acc att gat ggt 1411 Asp Ala Arg Leu Thr Pro Glu Val Arg Glu Val Leu Thr Ile Asp Gly 425 430 435 gca gtg gct tcc cgt gca acg cgc ggt gga acc gcg ggc gtg cgg gtt 1459 Ala Val Ala Ser Arg Ala Thr Arg Gly Gly Thr Ala Gly Val Arg Val 440 445 450 gcg gag caa cgc gca cgt gtc gat gcc gca agt acc gct cac gcg gag 1507 Ala Glu Gln Arg Ala Arg Val Asp Ala Ala Ser Thr Ala His Ala Glu 455 460 465 tgg gca cgt gcg ggg gta cgt cga taagcattag tttatggcct gtgctgcttt 1561 Trp Ala Arg Ala Gly Val Arg Arg 470 475 <210> 88 <211> 477 <212> PRT <213> Corynebacterium glutamicum <400> 88 Met Glu Gln His Gly Thr Asn Glu Gly Ala Leu Trp Gly Gly Arg Phe 1 5 10 15 Ser Gly Gly Pro Ser Glu Ala Met Phe Ala Leu Ser Val Ser Thr His 20 25 30 Phe Asp Trp Val Leu Ala Pro Tyr Asp Val Leu Ala Ser Lys Ala His 35 40 45 Ala Lys Val Leu His Gln Ala Asp Leu Leu Ser Asp Glu Asp Leu Ala 50 55 60 Thr Met Leu Ala Gly Leu Asp Gln Leu Gly Lys Asp Val Ala Asp Gly 65 70 75 80 Thr Phe Gly Pro Leu Pro Ser Asp Glu Asp Val His Gly Ala Met Glu 85 90 95 Arg Gly Leu Ile Asp Arg Val Gly Pro Glu Val Gly Gly Arg Leu Arg 100 105 110 Ala Gly Arg Ser Arg Asn Asp Gln Val Ala Thr Leu Phe Arg Met Trp 115 120 125 Val Arg Asp Ala Val Arg Asp Ile Ala Leu Gly Thr Thr Glu Leu Val 130 135 140 Asp Ala Leu Ser Ala Gln Ala Lys Ala His Ala Gly Ala Ile Met Pro 145 150 155 160 Gly Lys Thr His Phe Gln Ala Ala Gln Pro Val Leu Leu Ala His Gln 165 170 175 Leu Leu Ala His Ala Gln Pro Leu Leu Arg Asp Ile Asp Arg Ile Arg 180 185 190 Asp Leu Asp Lys Arg Leu Ala Val Ser Pro Tyr Gly Ser Gly Ala Leu 195 200 205 Ala Gly Ser Ser Leu Lys Leu Asn Pro Glu Ala Ile Ala Glu Glu Leu 210 215 220 Gly Phe Asp Ser Ala Ala Asp Asn Ser Ile Asp Ala Thr Ser Ser Arg 225 230 235 240 Asp Phe Ala Ser Glu Thr Ala Phe Val Leu Ala Gln Leu Ala Val Asp 245 250 255 Met Ser Arg Leu Ala Glu Glu Ile Ile Ala Trp Cys Thr Pro Glu Phe 260 265 270 Gly Tyr Ile Thr Leu Ser Asp Ser Trp Ser Thr Gly Ser Ser Ile Met 275 280 285 Pro Gln Lys Lys Asn Pro Asp Val Ala Glu Leu Thr Arg Gly Lys Ser 290 295 300 Gly Arg Leu Ile Gly Asn Leu Thr Gly Leu Leu Ala Thr Leu Lys Ala 305 310 315 320 Gln Pro Leu Ala Tyr Asn Arg Asp Leu Gln Glu Asp Lys Glu Pro Ile 325 330 335 Val Asp Ser Val Ala Gln Leu Asn Leu Leu Leu Pro Ala Met Thr Gly 340 345 350 Leu Val Ser Thr Leu Thr Phe Asn Thr Glu Arg Met Arg Glu Leu Ala 355 360 365 Pro Ala Gly Phe Thr Leu Ala Thr Asp Leu Ala Glu Trp Met Val Arg 370 375 380 Gln Gly Val Pro Phe Arg Glu Ala His Glu Ala Ser Gly Ala Cys Val 385 390 395 400 Arg Ile Ala Glu Ser Arg Gly Val Asp Leu Ile Asp Leu Thr Asp Glu 405 410 415 Glu Leu Ser Gly Val Asp Ala Arg Leu Thr Pro Glu Val Arg Glu Val 420 425 430 Leu Thr Ile Asp Gly Ala Val Ala Ser Arg Ala Thr Arg Gly Gly Thr 435 440 445 Ala Gly Val Arg Val Ala Glu Gln Arg Ala Arg Val Asp Ala Ala Ser 450 455 460 Thr Ala His Ala Glu Trp Ala Arg Ala Gly Val Arg Arg 465 470 475 <210> 89 <211> 1270 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1240) <223> RXA02176 <400> 89 gtgaagaatc tagggtgcta ggaactgaca gcttcagggt tatagttgtt gggtcagatc 60 gttaacgatc cctggccctt ttacttccaa gcgcagaaag ttg ccc gaa gac atg 115 Leu Pro Glu Asp Met 1 5 acc gac ttc ccc acc ctg ccc tct gag ttc atc cct ggc gac ggc cgt 163 Thr Asp Phe Pro Thr Leu Pro Ser Glu Phe Ile Pro Gly Asp Gly Arg 10 15 20 ttc ggc tgc gga cct tcc aag gtt cga cca gaa cag att cag gct att 211 Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu Gln Ile Gln Ala Ile 25 30 35 gtc gac gga tcc gca tcc gtc atc ggt acc tca cac cgt cag ccg gca 259 Val Asp Gly Ser Ala Ser Val Ile Gly Thr Ser His Arg Gln Pro Ala 40 45 50 gta aaa aac gtc gtg ggt tca atc cgc gag gga ctc tcc gac ctc ttc 307 Val Lys Asn Val Val Gly Ser Ile Arg Glu Gly Leu Ser Asp Leu Phe 55 60 65 tcc ctt cca gaa ggc tac gag atc atc ctt tcc cta ggt ggt gcg acc 355 Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser Leu Gly Gly Ala Thr 70 75 80 85 gca ttc tgg gat gca gca acc ttc gga ctc att gaa aag aag tcc ggt 403 Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile Glu Lys Lys Ser Gly 90 95 100 cac ctt tct ttc ggt gag ttc tcc tcc aag ttc gca aag gct tct aag 451 His Leu Ser Phe Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser Lys 105 110 115 ctt gct cct tgg ctc gac gag cca gag atc gtc acc gca gaa acc ggt 499 Leu Ala Pro Trp Leu Asp Glu Pro Glu Ile Val Thr Ala Glu Thr Gly 120 125 130 gac tct ccg gcc cca cag gca ttc gaa ggc gcc gat gtt att gca tgg 547 Asp Ser Pro Ala Pro Gln Ala Phe Glu Gly Ala Asp Val Ile Ala Trp 135 140 145 gca cac aac gaa acc tcc act ggc gcc atg gtt cca gtt ctt cgc ccc 595 Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Val Leu Arg Pro 150 155 160 165 gaa ggc tct gaa ggc tcc ctg gtt gcc att gac gca acc tcc ggc gct 643 Glu Gly Ser Glu Gly Ser Leu Val Ala Ile Asp Ala Thr Ser Gly Ala 170 175 180 ggt gga ctg cca gta gac atc aag aac tcc gat gtt tac tac ttc tcc 691 Gly Gly Leu Pro Val Asp Ile Lys Asn Ser Asp Val Tyr Tyr Phe Ser 185 190 195 cca cag aag tgc ttc gca tcc gac ggt ggc ctg tgg ctt gca gcg atg 739 Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala Met 200 205 210 agc cca gca gct ctc gag cgc atc gag aag atc aac gct tcc gat cgc 787 Ser Pro Ala Ala Leu Glu Arg Ile Glu Lys Ile Asn Ala Ser Asp Arg 215 220 225 ttc atc cct gag ttc ctc aac ctg cag acc gca gtg gat aac tcc ctg 835 Phe Ile Pro Glu Phe Leu Asn Leu Gln Thr Ala Val Asp Asn Ser Leu 230 235 240 245 aag aac cag acc tac aac acc cca gct gtt gct acc ttg ctg atg ctg 883 Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Ala Thr Leu Leu Met Leu 250 255 260 gac aac cag gtc aag tgg atg aac tcc aac ggc ggc ctg gat gga atg 931 Asp Asn Gln Val Lys Trp Met Asn Ser Asn Gly Gly Leu Asp Gly Met 265 270 275 gtt gct cgc acc aca gca agc tcc tcc gcc ctg tac aac tgg gct gag 979 Val Ala Arg Thr Thr Ala Ser Ser Ser Ala Leu Tyr Asn Trp Ala Glu 280 285 290 gct cgc gag gag gca tcc cca tac gtg gca gat gca gct aag cgc tcc 1027 Ala Arg Glu Glu Ala Ser Pro Tyr Val Ala Asp Ala Ala Lys Arg Ser 295 300 305 ctc gtt gtc ggc acc atc gac ttc gat gac tcc atc gac gca gca gtg 1075 Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Ile Asp Ala Ala Val 310 315 320 325 atc gct aag ata ctg cgc gca aac ggc atc ctg gac acc gag cct tac 1123 Ile Ala Lys Ile Leu Arg Ala Asn Gly Ile Leu Asp Thr Glu Pro Tyr 330 335 340 cgc aag ctg gga cgc aac cag ctg cgc atc ggt atg ttc cca gcg atc 1171 Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly Met Phe Pro Ala Ile 345 350 355 gat tcc acc gat gtg gaa aag ctc acc gga gca atc gac ttc atc ctc 1219 Asp Ser Thr Asp Val Glu Lys Leu Thr Gly Ala Ile Asp Phe Ile Leu 360 365 370 gat ggc ggt ttt gca agg aag taataccccc actttgaaaa acaccccgta 1270 Asp Gly Gly Phe Ala Arg Lys 375 380 <210> 90 <211> 380 <212> PRT <213> Corynebacterium glutamicum <400> 90 Leu Pro Glu Asp Met Thr Asp Phe Pro Thr Leu Pro Ser Glu Phe Ile 1 5 10 15 Pro Gly Asp Gly Arg Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu 20 25 30 Gln Ile Gln Ala Ile Val Asp Gly Ser Ala Ser Val Ile Gly Thr Ser 35 40 45 His Arg Gln Pro Ala Val Lys Asn Val Val Gly Ser Ile Arg Glu Gly 50 55 60 Leu Ser Asp Leu Phe Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser 65 70 75 80 Leu Gly Gly Ala Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile 85 90 95 Glu Lys Lys Ser Gly His Leu Ser Phe Gly Glu Phe Ser Ser Lys Phe 100 105 110 Ala Lys Ala Ser Lys Leu Ala Pro Trp Leu Asp Glu Pro Glu Ile Val 115 120 125 Thr Ala Glu Thr Gly Asp Ser Pro Ala Pro Gln Ala Phe Glu Gly Ala 130 135 140 Asp Val Ile Ala Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val 145 150 155 160 Pro Val Leu Arg Pro Glu Gly Ser Glu Gly Ser Leu Val Ala Ile Asp 165 170 175 Ala Thr Ser Gly Ala Gly Gly Leu Pro Val Asp Ile Lys Asn Ser Asp 180 185 190 Val Tyr Tyr Phe Ser Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu 195 200 205 Trp Leu Ala Ala Met Ser Pro Ala Ala Leu Glu Arg Ile Glu Lys Ile 210 215 220 Asn Ala Ser Asp Arg Phe Ile Pro Glu Phe Leu Asn Leu Gln Thr Ala 225 230 235 240 Val Asp Asn Ser Leu Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Ala 245 250 255 Thr Leu Leu Met Leu Asp Asn Gln Val Lys Trp Met Asn Ser Asn Gly 260 265 270 Gly Leu Asp Gly Met Val Ala Arg Thr Thr Ala Ser Ser Ser Ala Leu 275 280 285 Tyr Asn Trp Ala Glu Ala Arg Glu Glu Ala Ser Pro Tyr Val Ala Asp 290 295 300 Ala Ala Lys Arg Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser 305 310 315 320 Ile Asp Ala Ala Val Ile Ala Lys Ile Leu Arg Ala Asn Gly Ile Leu 325 330 335 Asp Thr Glu Pro Tyr Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly 340 345 350 Met Phe Pro Ala Ile Asp Ser Thr Asp Val Glu Lys Leu Thr Gly Ala 355 360 365 Ile Asp Phe Ile Leu Asp Gly Gly Phe Ala Arg Lys 370 375 380 <210> 91 <211> 973 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(943) <223> RXA02194 <400> 91 gaaatctccc agctcattta ttggacccag gtcatcatgg ttgctcgcgg cctgaagcca 60 gaagatatct acaagaacct gtaggagttt taaagcaatc atg ttg aaa atc gct 115 Met Leu Lys Ile Ala 1 5 gtc cca aac aaa ggc tcg ctg tcc gag cgc gcc atg gaa atc ctc gcc 163 Val Pro Asn Lys Gly Ser Leu Ser Glu Arg Ala Met Glu Ile Leu Ala 10 15 20 gaa gca ggc tac gca ggc cgt gga gat tcc aaa tcc ctc aac gtt ttt 211 Glu Ala Gly Tyr Ala Gly Arg Gly Asp Ser Lys Ser Leu Asn Val Phe 25 30 35 gat gaa gca aac aac gtt gaa ttc ttc ttc ctt cgc cct aaa gat atc 259 Asp Glu Ala Asn Asn Val Glu Phe Phe Phe Leu Arg Pro Lys Asp Ile 40 45 50 gcc atc tac gtt gct ggt ggc cag ctc gat ttg ggt atc acc ggc cgc 307 Ala Ile Tyr Val Ala Gly Gly Gln Leu Asp Leu Gly Ile Thr Gly Arg 55 60 65 gac ctt gct cgc gat tcc cag gct gat gtc cac gaa gtt ctt tcc ctc 355 Asp Leu Ala Arg Asp Ser Gln Ala Asp Val His Glu Val Leu Ser Leu 70 75 80 85 ggc ttc ggt tcc tcc act ttc cgt tac gca gca cca gct gat gaa gag 403 Gly Phe Gly Ser Ser Thr Phe Arg Tyr Ala Ala Pro Ala Asp Glu Glu 90 95 100 tgg agc atc gaa aag ctc gac ggc aag cgc atc gct acc tct tac ccc 451 Trp Ser Ile Glu Lys Leu Asp Gly Lys Arg Ile Ala Thr Ser Tyr Pro 105 110 115 aac ctt gtt cgc gat gac ctc gca gca cgt ggg ctt tcc gct gag gtg 499 Asn Leu Val Arg Asp Asp Leu Ala Ala Arg Gly Leu Ser Ala Glu Val 120 125 130 ctc cgc ctc gac ggt gca gta gag gta tcc atc aag ctt ggt gtc gca 547 Leu Arg Leu Asp Gly Ala Val Glu Val Ser Ile Lys Leu Gly Val Ala 135 140 145 gat gcc atc gcc gat gtt gta tcc acc ggc cgc acg ctg cgt cag caa 595 Asp Ala Ile Ala Asp Val Val Ser Thr Gly Arg Thr Leu Arg Gln Gln 150 155 160 165 ggt ctt gca cct ttc ggc gag gtt ctg tgc acc tct gag gct gtc att 643 Gly Leu Ala Pro Phe Gly Glu Val Leu Cys Thr Ser Glu Ala Val Ile 170 175 180 gtt ggc cgc aag gat gaa aag gtc acc cca gag cag cag atc ctg ctt 691 Val Gly Arg Lys Asp Glu Lys Val Thr Pro Glu Gln Gln Ile Leu Leu 185 190 195 cgc cgc atc cag gga att ttg cac gcg cag aac ttc ctc atg ctg gat 739 Arg Arg Ile Gln Gly Ile Leu His Ala Gln Asn Phe Leu Met Leu Asp 200 205 210 tac aac gtc gac cgc gac aac ctg gac gct gcc act gca gta acc cca 787 Tyr Asn Val Asp Arg Asp Asn Leu Asp Ala Ala Thr Ala Val Thr Pro 215 220 225 ggc tta tcc ggc cca acg gta tcc cca ctg gca cgc gac aac tgg gtt 835 Gly Leu Ser Gly Pro Thr Val Ser Pro Leu Ala Arg Asp Asn Trp Val 230 235 240 245 gct gta cgc gcc atg gtg cca cgc agg tca gct aac gcc atc atg gat 883 Ala Val Arg Ala Met Val Pro Arg Arg Ser Ala Asn Ala Ile Met Asp 250 255 260 aag ctt gct gga ctc ggc gct gaa gcc atc ctg gct tct gaa atc cgc 931 Lys Leu Ala Gly Leu Gly Ala Glu Ala Ile Leu Ala Ser Glu Ile Arg 265 270 275 atc gcc cgc atc tagttttaac tacccccgaa aatgtagtgg 973 Ile Ala Arg Ile 280 <210> 92 <211> 281 <212> PRT <213> Corynebacterium glutamicum <400> 92 Met Leu Lys Ile Ala Val Pro Asn Lys Gly Ser Leu Ser Glu Arg Ala 1 5 10 15 Met Glu Ile Leu Ala Glu Ala Gly Tyr Ala Gly Arg Gly Asp Ser Lys 20 25 30 Ser Leu Asn Val Phe Asp Glu Ala Asn Asn Val Glu Phe Phe Phe Leu 35 40 45 Arg Pro Lys Asp Ile Ala Ile Tyr Val Ala Gly Gly Gln Leu Asp Leu 50 55 60 Gly Ile Thr Gly Arg Asp Leu Ala Arg Asp Ser Gln Ala Asp Val His 65 70 75 80 Glu Val Leu Ser Leu Gly Phe Gly Ser Ser Thr Phe Arg Tyr Ala Ala 85 90 95 Pro Ala Asp Glu Glu Trp Ser Ile Glu Lys Leu Asp Gly Lys Arg Ile 100 105 110 Ala Thr Ser Tyr Pro Asn Leu Val Arg Asp Asp Leu Ala Ala Arg Gly 115 120 125 Leu Ser Ala Glu Val Leu Arg Leu Asp Gly Ala Val Glu Val Ser Ile 130 135 140 Lys Leu Gly Val Ala Asp Ala Ile Ala Asp Val Val Ser Thr Gly Arg 145 150 155 160 Thr Leu Arg Gln Gln Gly Leu Ala Pro Phe Gly Glu Val Leu Cys Thr 165 170 175 Ser Glu Ala Val Ile Val Gly Arg Lys Asp Glu Lys Val Thr Pro Glu 180 185 190 Gln Gln Ile Leu Leu Arg Arg Ile Gln Gly Ile Leu His Ala Gln Asn 195 200 205 Phe Leu Met Leu Asp Tyr Asn Val Asp Arg Asp Asn Leu Asp Ala Ala 210 215 220 Thr Ala Val Thr Pro Gly Leu Ser Gly Pro Thr Val Ser Pro Leu Ala 225 230 235 240 Arg Asp Asn Trp Val Ala Val Arg Ala Met Val Pro Arg Arg Ser Ala 245 250 255 Asn Ala Ile Met Asp Lys Leu Ala Gly Leu Gly Ala Glu Ala Ile Leu 260 265 270 Ala Ser Glu Ile Arg Ile Ala Arg Ile 275 280 <210> 93 <211> 1198 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1168) <223> RXA02206 <400> 93 gccggcatcg gcaccccaga acagctcaga gatgattcgg aggtgtccgc tggagctgat 60 cggcaggaac tctgtgaggc cttgaacgat ggagagcaca atg gtt tgt acc cag 115 Met Val Cys Thr Gln 1 5 cca ata ttt tca gtt gcg gca gga tct gct gct gcg gct agg agg gtt 163 Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala Ala Ala Arg Arg Val 10 15 20 atc tct tca ttc acc cga tct acc gta cta cct tat gac ctc agt agt 211 Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro Tyr Asp Leu Ser Ser 25 30 35 gtg gtg ggc gtg aaa cag cga atg gtc ggt tca agt ggt ttg cgg gta 259 Val Val Gly Val Lys Gln Arg Met Val Gly Ser Ser Gly Leu Arg Val 40 45 50 tcc agg ctc ggt ttg ggc acc tca aca tgg ggc tcg ggc acc gag ctg 307 Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly Ser Gly Thr Glu Leu 55 60 65 gct gag gca ggc gat atc ttt aag gcg ttc atc aat tct ggt ggc acg 355 Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile Asn Ser Gly Gly Thr 70 75 80 85 ctt atc gac gtc tcc ccc aac tac acc acc ggc gtc gcg gaa gaa atg 403 Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly Val Ala Glu Glu Met 90 95 100 ctc ggc acg atg ttg gat gcg gaa gtc tct cgt tcg gct gtc gtc att 451 Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg Ser Ala Val Val Ile 105 110 115 tcc tcc agc gca ggt gtc aac ccc gct ctg ccg ctc ggc cga cgt gtg 499 Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro Leu Gly Arg Arg Val 120 125 130 gat tgc tcc cgc cgc aat ttg att gcc caa tta gat gtc acc ctg cgg 547 Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu Asp Val Thr Leu Arg 135 140 145 gca tta aac act gac tat ttg gat ttg tgg tct gtg ggc tat tgg gat 595 Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser Val Gly Tyr Trp Asp 150 155 160 165 gag ggc acc cca ccg cat gag gtg gcc gat act ttg gat tac gcc gtg 643 Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr Leu Asp Tyr Ala Val 170 175 180 cgc acc ggc cga gtc cga tat gcc ggt gtc cga gga tat tcc ggt tgg 691 Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg Gly Tyr Ser Gly Trp 185 190 195 cag tta gcg gtc acc cac gct gca tcc aat cat gca gcg gcc tcc gcc 739 Gln Leu Ala Val Thr His Ala Ala Ser Asn His Ala Ala Ala Ser Ala 200 205 210 cgc ccc gtg gtc gtt gca caa aat gaa tac agc ctg ctg gaa cgc cgc 787 Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser Leu Leu Glu Arg Arg 215 220 225 gca gaa caa gaa ctc ctc cct gcc acc caa cac cta ggt gtc gga ttc 835 Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His Leu Gly Val Gly Phe 230 235 240 245 ttt gct ggc gct ccg ctg ggg caa ggc gtg ctg act gct aaa tac cgc 883 Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu Thr Ala Lys Tyr Arg 250 255 260 tcc gaa att ccc cat gat tcc aga gct gca tcc aca gga cgc gac gca 931 Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser Thr Gly Arg Asp Ala 265 270 275 gaa gtc caa agc tac cta gat aat cga ggc cgc atc att gtc gat gct 979 Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg Ile Ile Val Asp Ala 280 285 290 ctt gat act gca gcc aaa gga tta ggc att agc ccc gct gtc aca gcc 1027 Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser Pro Ala Val Thr Ala 295 300 305 acc acc tgg gtg cgt gat cgt ccc gga gtg aca gct gtc atc gtg ggc 1075 Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr Ala Val Ile Val Gly 310 315 320 325 gct cgc aca cat gaa cag ctg tca cat ctt ctc aag gcg gaa tcg gtg 1123 Ala Arg Thr His Glu Gln Leu Ser His Leu Leu Lys Ala Glu Ser Val 330 335 340 act ttg cca aca cca atc aca caa gcc ctt gat gat gtc tcc ctg 1168 Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp Asp Val Ser Leu 345 350 355 tgacttggtc caattacatt cactggtaat 1198 <210> 94 <211> 356 <212> PRT <213> Corynebacterium glutamicum <400> 94 Met Val Cys Thr Gln Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala 1 5 10 15 Ala Ala Arg Arg Val Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro 20 25 30 Tyr Asp Leu Ser Ser Val Val Gly Val Lys Gln Arg Met Val Gly Ser 35 40 45 Ser Gly Leu Arg Val Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly 50 55 60 Ser Gly Thr Glu Leu Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile 65 70 75 80 Asn Ser Gly Gly Thr Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly 85 90 95 Val Ala Glu Glu Met Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg 100 105 110 Ser Ala Val Val Ile Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro 115 120 125 Leu Gly Arg Arg Val Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu 130 135 140 Asp Val Thr Leu Arg Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser 145 150 155 160 Val Gly Tyr Trp Asp Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr 165 170 175 Leu Asp Tyr Ala Val Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg 180 185 190 Gly Tyr Ser Gly Trp Gln Leu Ala Val Thr His Ala Ala Ser Asn His 195 200 205 Ala Ala Ala Ser Ala Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser 210 215 220 Leu Leu Glu Arg Arg Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His 225 230 235 240 Leu Gly Val Gly Phe Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu 245 250 255 Thr Ala Lys Tyr Arg Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser 260 265 270 Thr Gly Arg Asp Ala Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg 275 280 285 Ile Ile Val Asp Ala Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser 290 295 300 Pro Ala Val Thr Ala Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr 305 310 315 320 Ala Val Ile Val Gly Ala Arg Thr His Glu Gln Leu Ser His Leu Leu 325 330 335 Lys Ala Glu Ser Val Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp 340 345 350 Asp Val Ser Leu 355 <210> 95 <211> 700 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(670) <223> RXA02237 <400> 95 gacgagctgg gcattgctca gacccgtcgt cttcgtggac tgggtgaccg tcagcgtcgc 60 gcacttctcg agcgtttcgg cttcgaggat taattcttca gtg tcg ggc gat aac 115 Val Ser Gly Asp Asn 1 5 caa cta gga cgg ctc gta att ctt gcg ggc ccc tca gcg gtc ggt aaa 163 Gln Leu Gly Arg Leu Val Ile Leu Ala Gly Pro Ser Ala Val Gly Lys 10 15 20 tcg act gtg gtt gat cgc ctc cgc aat gac gtt cca aac ctg tat ttc 211 Ser Thr Val Val Asp Arg Leu Arg Asn Asp Val Pro Asn Leu Tyr Phe 25 30 35 agt gtg tcg atg acc act agg gca cct cgt cct ggt gaa gtc gat gga 259 Ser Val Ser Met Thr Thr Arg Ala Pro Arg Pro Gly Glu Val Asp Gly 40 45 50 cgt gac tac ttc tat gtc act gca cag gaa ttt cag gac aaa atc gac 307 Arg Asp Tyr Phe Tyr Val Thr Ala Gln Glu Phe Gln Asp Lys Ile Asp 55 60 65 tgt gga gag atg ctt gaa tgg gca gat atc cac ggc ggt ttg cag cgt 355 Cys Gly Glu Met Leu Glu Trp Ala Asp Ile His Gly Gly Leu Gln Arg 70 75 80 85 tca ggc act cca gca ggt ccc gtc aat gag gct cgc caa aat ggt cgg 403 Ser Gly Thr Pro Ala Gly Pro Val Asn Glu Ala Arg Gln Asn Gly Arg 90 95 100 cca gta ttg gtt gag gtt gat ctt gca gga gcc cga aac atc gct agc 451 Pro Val Leu Val Glu Val Asp Leu Ala Gly Ala Arg Asn Ile Ala Ser 105 110 115 tta att cca gat gca gaa acc atc ttc ctt gct cca cct tca tgg gaa 499 Leu Ile Pro Asp Ala Glu Thr Ile Phe Leu Ala Pro Pro Ser Trp Glu 120 125 130 gtt ttg gtt gaa cgc ctc act gga cgt ggc acc gaa agc gaa gac gtt 547 Val Leu Val Glu Arg Leu Thr Gly Arg Gly Thr Glu Ser Glu Asp Val 135 140 145 att gct cgc agg ctc gag acc gca cgc gaa gaa ttg gct gct cag agc 595 Ile Ala Arg Arg Leu Glu Thr Ala Arg Glu Glu Leu Ala Ala Gln Ser 150 155 160 165 gaa ttt aag cac gtc att atc aat gat gat gtg gat aca gcc gtc aag 643 Glu Phe Lys His Val Ile Ile Asn Asp Asp Val Asp Thr Ala Val Lys 170 175 180 gct att gag gat gtt ctc ctc ggc gct tagccaaaac atagagcggt 690 Ala Ile Glu Asp Val Leu Leu Gly Ala 185 190 agggtatgct 700 <210> 96 <211> 190 <212> PRT <213> Corynebacterium glutamicum <400> 96 Val Ser Gly Asp Asn Gln Leu Gly Arg Leu Val Ile Leu Ala Gly Pro 1 5 10 15 Ser Ala Val Gly Lys Ser Thr Val Val Asp Arg Leu Arg Asn Asp Val 20 25 30 Pro Asn Leu Tyr Phe Ser Val Ser Met Thr Thr Arg Ala Pro Arg Pro 35 40 45 Gly Glu Val Asp Gly Arg Asp Tyr Phe Tyr Val Thr Ala Gln Glu Phe 50 55 60 Gln Asp Lys Ile Asp Cys Gly Glu Met Leu Glu Trp Ala Asp Ile His 65 70 75 80 Gly Gly Leu Gln Arg Ser Gly Thr Pro Ala Gly Pro Val Asn Glu Ala 85 90 95 Arg Gln Asn Gly Arg Pro Val Leu Val Glu Val Asp Leu Ala Gly Ala 100 105 110 Arg Asn Ile Ala Ser Leu Ile Pro Asp Ala Glu Thr Ile Phe Leu Ala 115 120 125 Pro Pro Ser Trp Glu Val Leu Val Glu Arg Leu Thr Gly Arg Gly Thr 130 135 140 Glu Ser Glu Asp Val Ile Ala Arg Arg Leu Glu Thr Ala Arg Glu Glu 145 150 155 160 Leu Ala Ala Gln Ser Glu Phe Lys His Val Ile Ile Asn Asp Asp Val 165 170 175 Asp Thr Ala Val Lys Ala Ile Glu Asp Val Leu Leu Gly Ala 180 185 190 <210> 97 <211> 1396 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1366) <223> RXA02239 <400> 97 ctatgcacag cggaggtcga gggagagcgt ggggtggcgt cgaaaagcat cttgtagagt 60 gtgtgggaac ccagccagcc tcttactttg aaggattgtt gtg ccc atg act cat 115 Val Pro Met Thr His 1 5 gtt tca agc ccc tcc gca ccc cgc aac gtg gtt gtt ggt gtt gcc ggc 163 Val Ser Ser Pro Ser Ala Pro Arg Asn Val Val Val Gly Val Ala Gly 10 15 20 gga atc gca gcg tac aag gcg tgt cac atc gtg cgc gcg ttt aaa gaa 211 Gly Ile Ala Ala Tyr Lys Ala Cys His Ile Val Arg Ala Phe Lys Glu 25 30 35 gcg ggc gat aat gtg cgg gtg gtt cct acg gaa tcc gcg ttg aag ttt 259 Ala Gly Asp Asn Val Arg Val Val Pro Thr Glu Ser Ala Leu Lys Phe 40 45 50 gtg ggg aag gcg acg ttt gaa gcg ttg tct ggc aat ccg gtg tct aca 307 Val Gly Lys Ala Thr Phe Glu Ala Leu Ser Gly Asn Pro Val Ser Thr 55 60 65 acg gtg ttt gat gcg gtg gat tcg gtg cag cat gtg aaa gtt ggc cag 355 Thr Val Phe Asp Ala Val Asp Ser Val Gln His Val Lys Val Gly Gln 70 75 80 85 gag gct gat ttg atc gtg att gcg ccg gcg aca gcc gat ttg atg gcg 403 Glu Ala Asp Leu Ile Val Ile Ala Pro Ala Thr Ala Asp Leu Met Ala 90 95 100 cgt gtg gtg gca ggt cgc ggt gac gat ctg ttg gcg gcg acg ctg ctg 451 Arg Val Val Ala Gly Arg Gly Asp Asp Leu Leu Ala Ala Thr Leu Leu 105 110 115 gtg gca acg tgc ccc gtg gtt att gcg ccg gcc atg cat acg gag atg 499 Val Ala Thr Cys Pro Val Val Ile Ala Pro Ala Met His Thr Glu Met 120 125 130 tgg ttt aat ccg gct acc gta gcc aat gtg gca acg ctg agg cag cgg 547 Trp Phe Asn Pro Ala Thr Val Ala Asn Val Ala Thr Leu Arg Gln Arg 135 140 145 ggg att acc gtg att gag cct gcg cat ggt cga ctc acc ggt aaa gat 595 Gly Ile Thr Val Ile Glu Pro Ala His Gly Arg Leu Thr Gly Lys Asp 150 155 160 165 aca ggc cct ggc cgg ctg ccg gat cca gag cag att gtt gat tta gcc 643 Thr Gly Pro Gly Arg Leu Pro Asp Pro Glu Gln Ile Val Asp Leu Ala 170 175 180 aat gcg gtg cac gcc ggg gcg agg ttg cct cag gat ttg gcg ggc aag 691 Asn Ala Val His Ala Gly Ala Arg Leu Pro Gln Asp Leu Ala Gly Lys 185 190 195 aaa gtg ctg atc act gct ggt ggc acg cat gag cat att gat cct gtg 739 Lys Val Leu Ile Thr Ala Gly Gly Thr His Glu His Ile Asp Pro Val 200 205 210 cgc ttt att ggc aat agt tcc tcg ggc cgt caa ggt ttt gcg ttg ggt 787 Arg Phe Ile Gly Asn Ser Ser Ser Gly Arg Gln Gly Phe Ala Leu Gly 215 220 225 gaa atc gca gca cag cga ggt gct cat gtc agc atc gtg gcg gga aat 835 Glu Ile Ala Ala Gln Arg Gly Ala His Val Ser Ile Val Ala Gly Asn 230 235 240 245 gct gcg gag ctg ccc act ccg gca ggc gca gag atc gtg ccg gtg gtg 883 Ala Ala Glu Leu Pro Thr Pro Ala Gly Ala Glu Ile Val Pro Val Val 250 255 260 tcc aca caa gac atg ttt gat gca gtc cag gaa cga gct ggc caa tct 931 Ser Thr Gln Asp Met Phe Asp Ala Val Gln Glu Arg Ala Gly Gln Ser 265 270 275 gat ttc atc gtc atg gcg gca gcg gta gct gat ttc acg ccc gca tcg 979 Asp Phe Ile Val Met Ala Ala Ala Val Ala Asp Phe Thr Pro Ala Ser 280 285 290 cag gcg aca tcg aag ttg aag aag ggc tca gat tct gat gaa gac gca 1027 Gln Ala Thr Ser Lys Leu Lys Lys Gly Ser Asp Ser Asp Glu Asp Ala 295 300 305 ttg agc acc atc tcg ttg gtg gaa aac ccg gat att ttg gct acc acg 1075 Leu Ser Thr Ile Ser Leu Val Glu Asn Pro Asp Ile Leu Ala Thr Thr 310 315 320 325 gtg aag cgt cgt gaa gca gga gag ctg gac agt aat cct gtc atc gtg 1123 Val Lys Arg Arg Glu Ala Gly Glu Leu Asp Ser Asn Pro Val Ile Val 330 335 340 ggt ttt gct gcg gaa act gga gac gag cac acc acc gcc ttg gag tat 1171 Gly Phe Ala Ala Glu Thr Gly Asp Glu His Thr Thr Ala Leu Glu Tyr 345 350 355 gcg cgc aag aaa ctg cag aag aag ggc tgc gac ctc ctc atg tgt aat 1219 Ala Arg Lys Lys Leu Gln Lys Lys Gly Cys Asp Leu Leu Met Cys Asn 360 365 370 gag gtg ggc atg ggc aaa gtg ttt ggg caa aag cac aat gag ggc tgg 1267 Glu Val Gly Met Gly Lys Val Phe Gly Gln Lys His Asn Glu Gly Trp 375 380 385 att ttg gat gct cac ggt ggg gta gtc gat gtg gag cac ggc agc aaa 1315 Ile Leu Asp Ala His Gly Gly Val Val Asp Val Glu His Gly Ser Lys 390 395 400 405 atc gag gtt gct gcg caa att tgg gac gcg gca ctg gcg tat cgc gaa 1363 Ile Glu Val Ala Ala Gln Ile Trp Asp Ala Ala Leu Ala Tyr Arg Glu 410 415 420 gtc tagaaaaatc cagctagacc actgacattg 1396 Val <210> 98 <211> 422 <212> PRT <213> Corynebacterium glutamicum <400> 98 Val Pro Met Thr His Val Ser Ser Pro Ser Ala Pro Arg Asn Val Val 1 5 10 15 Val Gly Val Ala Gly Gly Ile Ala Ala Tyr Lys Ala Cys His Ile Val 20 25 30 Arg Ala Phe Lys Glu Ala Gly Asp Asn Val Arg Val Val Pro Thr Glu 35 40 45 Ser Ala Leu Lys Phe Val Gly Lys Ala Thr Phe Glu Ala Leu Ser Gly 50 55 60 Asn Pro Val Ser Thr Thr Val Phe Asp Ala Val Asp Ser Val Gln His 65 70 75 80 Val Lys Val Gly Gln Glu Ala Asp Leu Ile Val Ile Ala Pro Ala Thr 85 90 95 Ala Asp Leu Met Ala Arg Val Val Ala Gly Arg Gly Asp Asp Leu Leu 100 105 110 Ala Ala Thr Leu Leu Val Ala Thr Cys Pro Val Val Ile Ala Pro Ala 115 120 125 Met His Thr Glu Met Trp Phe Asn Pro Ala Thr Val Ala Asn Val Ala 130 135 140 Thr Leu Arg Gln Arg Gly Ile Thr Val Ile Glu Pro Ala His Gly Arg 145 150 155 160 Leu Thr Gly Lys Asp Thr Gly Pro Gly Arg Leu Pro Asp Pro Glu Gln 165 170 175 Ile Val Asp Leu Ala Asn Ala Val His Ala Gly Ala Arg Leu Pro Gln 180 185 190 Asp Leu Ala Gly Lys Lys Val Leu Ile Thr Ala Gly Gly Thr His Glu 195 200 205 His Ile Asp Pro Val Arg Phe Ile Gly Asn Ser Ser Ser Gly Arg Gln 210 215 220 Gly Phe Ala Leu Gly Glu Ile Ala Ala Gln Arg Gly Ala His Val Ser 225 230 235 240 Ile Val Ala Gly Asn Ala Ala Glu Leu Pro Thr Pro Ala Gly Ala Glu 245 250 255 Ile Val Pro Val Val Ser Thr Gln Asp Met Phe Asp Ala Val Gln Glu 260 265 270 Arg Ala Gly Gln Ser Asp Phe Ile Val Met Ala Ala Ala Val Ala Asp 275 280 285 Phe Thr Pro Ala Ser Gln Ala Thr Ser Lys Leu Lys Lys Gly Ser Asp 290 295 300 Ser Asp Glu Asp Ala Leu Ser Thr Ile Ser Leu Val Glu Asn Pro Asp 305 310 315 320 Ile Leu Ala Thr Thr Val Lys Arg Arg Glu Ala Gly Glu Leu Asp Ser 325 330 335 Asn Pro Val Ile Val Gly Phe Ala Ala Glu Thr Gly Asp Glu His Thr 340 345 350 Thr Ala Leu Glu Tyr Ala Arg Lys Lys Leu Gln Lys Lys Gly Cys Asp 355 360 365 Leu Leu Met Cys Asn Glu Val Gly Met Gly Lys Val Phe Gly Gln Lys 370 375 380 His Asn Glu Gly Trp Ile Leu Asp Ala His Gly Gly Val Val Asp Val 385 390 395 400 Glu His Gly Ser Lys Ile Glu Val Ala Ala Gln Ile Trp Asp Ala Ala 405 410 415 Leu Ala Tyr Arg Glu Val 420 <210> 99 <211> 709 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(679) <223> RXA02250 <400> 99 caactgaggg ttagactgtt ctttaaaagg tttgacgcct cggtttaatg cctagcagga 60 tgcgcgccac cgctaattag ttctaaggat ttgtgaaatc gtg aca acc aac gcc 115 Val Thr Thr Asn Ala 1 5 ccc gac gga gca acg aac aac atc aac aac gca cat tcg ggc gct gtc 163 Pro Asp Gly Ala Thr Asn Asn Ile Asn Asn Ala His Ser Gly Ala Val 10 15 20 ggg aag cca aag gta cag ctc agc gat gcg gaa att cag gaa tac acc 211 Gly Lys Pro Lys Val Gln Leu Ser Asp Ala Glu Ile Gln Glu Tyr Thr 25 30 35 gca gct ttc gct ggc acc acc aca acc aag cca tgg gag ctg gag gtg 259 Ala Ala Phe Ala Gly Thr Thr Thr Thr Lys Pro Trp Glu Leu Glu Val 40 45 50 acc aca aag ttt ctg aaa aag atc gcg tgg gta gcc gtg gtt gtc atc 307 Thr Thr Lys Phe Leu Lys Lys Ile Ala Trp Val Ala Val Val Val Ile 55 60 65 atg gcc gtt cac atc ttc atg ggt gcc gtg gtg gac gtc gat ttc acc 355 Met Ala Val His Ile Phe Met Gly Ala Val Val Asp Val Asp Phe Thr 70 75 80 85 ggt gca gcg gtc acc ttt gtt gac act ctg gca ttc cca gcg ttg ggc 403 Gly Ala Ala Val Thr Phe Val Asp Thr Leu Ala Phe Pro Ala Leu Gly 90 95 100 atc atc ttc tcc gtt ctt gtg ttc ttg gga ctg act cgc cct cgc gtg 451 Ile Ile Phe Ser Val Leu Val Phe Leu Gly Leu Thr Arg Pro Arg Val 105 110 115 cgt gcc aac gaa gac ggc gtt gag gtg cgt aac ttc atc gga act cgt 499 Arg Ala Asn Glu Asp Gly Val Glu Val Arg Asn Phe Ile Gly Thr Arg 120 125 130 ttc tac cca tgg gtt gtc atc tac ggc atg tct ttc ccc aag ggc agc 547 Phe Tyr Pro Trp Val Val Ile Tyr Gly Met Ser Phe Pro Lys Gly Ser 135 140 145 agc gtg gca cgt ttg gag ctt cca gac ttt gaa ttc gtt ccc atg tgg 595 Ser Val Ala Arg Leu Glu Leu Pro Asp Phe Glu Phe Val Pro Met Trp 150 155 160 165 gct ttc cag tcc cgc gat gga gaa gat gtg gtg cgc gcg gtt gcg acc 643 Ala Phe Gln Ser Arg Asp Gly Glu Asp Val Val Arg Ala Val Ala Thr 170 175 180 ttc cgc gac ctc gaa aac aag tac atg cca gag gac taattaagct 689 Phe Arg Asp Leu Glu Asn Lys Tyr Met Pro Glu Asp 185 190 gtggctgatc caacaactta 709 <210> 100 <211> 193 <212> PRT <213> Corynebacterium glutamicum <400> 100 Val Thr Thr Asn Ala Pro Asp Gly Ala Thr Asn Asn Ile Asn Asn Ala 1 5 10 15 His Ser Gly Ala Val Gly Lys Pro Lys Val Gln Leu Ser Asp Ala Glu 20 25 30 Ile Gln Glu Tyr Thr Ala Ala Phe Ala Gly Thr Thr Thr Thr Lys Pro 35 40 45 Trp Glu Leu Glu Val Thr Thr Lys Phe Leu Lys Lys Ile Ala Trp Val 50 55 60 Ala Val Val Val Ile Met Ala Val His Ile Phe Met Gly Ala Val Val 65 70 75 80 Asp Val Asp Phe Thr Gly Ala Ala Val Thr Phe Val Asp Thr Leu Ala 85 90 95 Phe Pro Ala Leu Gly Ile Ile Phe Ser Val Leu Val Phe Leu Gly Leu 100 105 110 Thr Arg Pro Arg Val Arg Ala Asn Glu Asp Gly Val Glu Val Arg Asn 115 120 125 Phe Ile Gly Thr Arg Phe Tyr Pro Trp Val Val Ile Tyr Gly Met Ser 130 135 140 Phe Pro Lys Gly Ser Ser Val Ala Arg Leu Glu Leu Pro Asp Phe Glu 145 150 155 160 Phe Val Pro Met Trp Ala Phe Gln Ser Arg Asp Gly Glu Asp Val Val 165 170 175 Arg Ala Val Ala Thr Phe Arg Asp Leu Glu Asn Lys Tyr Met Pro Glu 180 185 190 Asp <210> 101 <211> 910 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(880) <223> RXA02295 <400> 101 ttcccatgct aatccgcgaa aatggcactt caacagccat atactttaaa caacacaaat 60 gatgcaagac tgataccggg atgtgatagg agcgcaccac atg ggg ttg gaa tta 115 Met Gly Leu Glu Leu 1 5 gca gct agc ggg tgg ggc atc ctc atc gca ggc gcc gcc gta gcc gga 163 Ala Ala Ser Gly Trp Gly Ile Leu Ile Ala Gly Ala Ala Val Ala Gly 10 15 20 tgg atc gac gca gta atc ggc ggt ggc gga ctc gtc ctc atc ccg ctg 211 Trp Ile Asp Ala Val Ile Gly Gly Gly Gly Leu Val Leu Ile Pro Leu 25 30 35 atc ctc gcg gtc atg ccg caa ctc gca cct gtg aca gcg ctg gcc tcc 259 Ile Leu Ala Val Met Pro Gln Leu Ala Pro Val Thr Ala Leu Ala Ser 40 45 50 aac aaa ctg gca gcc gtc acc ggc acg gca tcg gcg gca ttc acc ctg 307 Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser Ala Ala Phe Thr Leu 55 60 65 gtc agg cgc gtc aaa ccc gac aaa aaa ctg ctt gcg ctc tac gtt ctg 355 Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu Ala Leu Tyr Val Leu 70 75 80 85 gtg gca gct gtg tgc tcc ggt gca ggc gcc ctg gct gcg agt ctc att 403 Val Ala Ala Val Cys Ser Gly Ala Gly Ala Leu Ala Ala Ser Leu Ile 90 95 100 gac aaa caa atc atg cga ccg ctg atc atc gtg ttg atg ctg gtc gtt 451 Asp Lys Gln Ile Met Arg Pro Leu Ile Ile Val Leu Met Leu Val Val 105 110 115 ggc ctg atc gtg gtg ttc aaa cca aac ttc gga acc ggc gaa agc aaa 499 Gly Leu Ile Val Val Phe Lys Pro Asn Phe Gly Thr Gly Glu Ser Lys 120 125 130 gcc ctg ccc acc gga tgg aaa cgc tgg gcc gcc atc gtt gca gtc gga 547 Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala Ile Val Ala Val Gly 135 140 145 ctc atc gca gcc tac gac ggc atc ttc gga ccc gga acc ggc atg ttc 595 Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro Gly Thr Gly Met Phe 150 155 160 165 ctc atc atg gcg ttc acc gca ctg ctc tcc caa aat ttc ctg tcc tcc 643 Leu Ile Met Ala Phe Thr Ala Leu Leu Ser Gln Asn Phe Leu Ser Ser 170 175 180 gca gcc atg gcg aag gtc gta aac acc gca aca aac ctg ggt gcg cta 691 Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr Asn Leu Gly Ala Leu 185 190 195 att gta ttc atc atc ggc ggc cac atg tgg tgg acc cta gga ctc gtg 739 Ile Val Phe Ile Ile Gly Gly His Met Trp Trp Thr Leu Gly Leu Val 200 205 210 ctg gca gtc gcc aat gtc gca ggc gca caa ctc ggt gcc cga acg gtg 787 Leu Ala Val Ala Asn Val Ala Gly Ala Gln Leu Gly Ala Arg Thr Val 215 220 225 ctt ggt ggc ggt acc agg cta att aga tac gca cta cta acc ctg gtt 835 Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala Leu Leu Thr Leu Val 230 235 240 245 gtc gtc atg agc gtc tac ctc acc tgg caa caa atc caa gga atg 880 Val Val Met Ser Val Tyr Leu Thr Trp Gln Gln Ile Gln Gly Met 250 255 260 tagataagtc ggggcaaact ctaagaaatc 910 <210> 102 <211> 260 <212> PRT <213> Corynebacterium glutamicum <400> 102 Met Gly Leu Glu Leu Ala Ala Ser Gly Trp Gly Ile Leu Ile Ala Gly 1 5 10 15 Ala Ala Val Ala Gly Trp Ile Asp Ala Val Ile Gly Gly Gly Gly Leu 20 25 30 Val Leu Ile Pro Leu Ile Leu Ala Val Met Pro Gln Leu Ala Pro Val 35 40 45 Thr Ala Leu Ala Ser Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser 50 55 60 Ala Ala Phe Thr Leu Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu 65 70 75 80 Ala Leu Tyr Val Leu Val Ala Ala Val Cys Ser Gly Ala Gly Ala Leu 85 90 95 Ala Ala Ser Leu Ile Asp Lys Gln Ile Met Arg Pro Leu Ile Ile Val 100 105 110 Leu Met Leu Val Val Gly Leu Ile Val Val Phe Lys Pro Asn Phe Gly 115 120 125 Thr Gly Glu Ser Lys Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala 130 135 140 Ile Val Ala Val Gly Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro 145 150 155 160 Gly Thr Gly Met Phe Leu Ile Met Ala Phe Thr Ala Leu Leu Ser Gln 165 170 175 Asn Phe Leu Ser Ser Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr 180 185 190 Asn Leu Gly Ala Leu Ile Val Phe Ile Ile Gly Gly His Met Trp Trp 195 200 205 Thr Leu Gly Leu Val Leu Ala Val Ala Asn Val Ala Gly Ala Gln Leu 210 215 220 Gly Ala Arg Thr Val Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala 225 230 235 240 Leu Leu Thr Leu Val Val Val Met Ser Val Tyr Leu Thr Trp Gln Gln 245 250 255 Ile Gln Gly Met 260 <210> 103 <211> 538 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(508) <223> RXA02299 <400> 103 acgcgggggt tgttgccgga tcgaaatatt cctttccttg tcatctcacg ctatgatttc 60 taaaacttgc aggacaaccc ccataaggac accacaggac atg ctg cgc acc atc 115 Met Leu Arg Thr Ile 1 5 ctc gga agt aag att cac cga gcc act gtc act caa gct gat cta gat 163 Leu Gly Ser Lys Ile His Arg Ala Thr Val Thr Gln Ala Asp Leu Asp 10 15 20 tat gtt ggc tct gta acc atc gac gcc gac ctg gtt cac gcc gcc gga 211 Tyr Val Gly Ser Val Thr Ile Asp Ala Asp Leu Val His Ala Ala Gly 25 30 35 ttg atc gaa ggc gaa aaa gtt gcc atc gta gac atc acc aac ggc gct 259 Leu Ile Glu Gly Glu Lys Val Ala Ile Val Asp Ile Thr Asn Gly Ala 40 45 50 cgt ctg gaa act tat gtc att gtg ggc gac gcc gga acg ggc aat att 307 Arg Leu Glu Thr Tyr Val Ile Val Gly Asp Ala Gly Thr Gly Asn Ile 55 60 65 tgc atc aat ggt gcc gct gca cac ctt att aat cct ggc gat ctt gtg 355 Cys Ile Asn Gly Ala Ala Ala His Leu Ile Asn Pro Gly Asp Leu Val 70 75 80 85 atc atc atg agc tac ctt cag gca act gat gcg gaa gcc aag gcg tat 403 Ile Ile Met Ser Tyr Leu Gln Ala Thr Asp Ala Glu Ala Lys Ala Tyr 90 95 100 gag cca aag att gtg cac gtg gac gcc gac aac cgc atc gtt gcg ctc 451 Glu Pro Lys Ile Val His Val Asp Ala Asp Asn Arg Ile Val Ala Leu 105 110 115 ggc aac gat ctt gcg gaa gca cta cct gga tcc ggg ctt ttg acg tcg 499 Gly Asn Asp Leu Ala Glu Ala Leu Pro Gly Ser Gly Leu Leu Thr Ser 120 125 130 aga agc att tagcgtttta gctcgccaat attgctgccg 538 Arg Ser Ile 135 <210> 104 <211> 136 <212> PRT <213> Corynebacterium glutamicum <400> 104 Met Leu Arg Thr Ile Leu Gly Ser Lys Ile His Arg Ala Thr Val Thr 1 5 10 15 Gln Ala Asp Leu Asp Tyr Val Gly Ser Val Thr Ile Asp Ala Asp Leu 20 25 30 Val His Ala Ala Gly Leu Ile Glu Gly Glu Lys Val Ala Ile Val Asp 35 40 45 Ile Thr Asn Gly Ala Arg Leu Glu Thr Tyr Val Ile Val Gly Asp Ala 50 55 60 Gly Thr Gly Asn Ile Cys Ile Asn Gly Ala Ala Ala His Leu Ile Asn 65 70 75 80 Pro Gly Asp Leu Val Ile Ile Met Ser Tyr Leu Gln Ala Thr Asp Ala 85 90 95 Glu Ala Lys Ala Tyr Glu Pro Lys Ile Val His Val Asp Ala Asp Asn 100 105 110 Arg Ile Val Ala Leu Gly Asn Asp Leu Ala Glu Ala Leu Pro Gly Ser 115 120 125 Gly Leu Leu Thr Ser Arg Ser Ile 130 135 <210> 105 <211> 1426 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1396) <223> RXA02382 <400> 105 gagcagatgg ccaccgaggt cgatgtggtg gctggctact aggcctttta tggtgtgatc 60 cgacactgtt gcttcttaag ggtctgtata gtgggcaacc atg agt tca acg acc 115 Met Ser Ser Thr Thr 1 5 cta act gat gac caa att cgc gac aat gag cgg acc gaa gtt cta gct 163 Leu Thr Asp Asp Gln Ile Arg Asp Asn Glu Arg Thr Glu Val Leu Ala 10 15 20 aaa gca act gca gct aag aac atc gtc ccg gat att gca gtg ttg ggc 211 Lys Ala Thr Ala Ala Lys Asn Ile Val Pro Asp Ile Ala Val Leu Gly 25 30 35 acc gga ccg aag aac gca atc ctg cgt gcg gcg gca gat gaa ctc gtt 259 Thr Gly Pro Lys Asn Ala Ile Leu Arg Ala Ala Ala Asp Glu Leu Val 40 45 50 gca cgc agc gca gaa atc atc gaa gcc aac gct tcc gat atc gaa gcg 307 Ala Arg Ser Ala Glu Ile Ile Glu Ala Asn Ala Ser Asp Ile Glu Ala 55 60 65 ggt cgc gca aac ggc atg gaa gaa tcc atg att gat cgc ctt gcc ctt 355 Gly Arg Ala Asn Gly Met Glu Glu Ser Met Ile Asp Arg Leu Ala Leu 70 75 80 85 gat gaa tct cgc att gag ggc atc gct ggc ggt ttg cgc cag gtt gct 403 Asp Glu Ser Arg Ile Glu Gly Ile Ala Gly Gly Leu Arg Gln Val Ala 90 95 100 ggc ctg acc gac cca gtg ggt gaa gta ctg cgc gga cat gtc atg gaa 451 Gly Leu Thr Asp Pro Val Gly Glu Val Leu Arg Gly His Val Met Glu 105 110 115 aac ggc att cag atg aag cag gtc cgt gtg cct ttg ggc gtg atg ggc 499 Asn Gly Ile Gln Met Lys Gln Val Arg Val Pro Leu Gly Val Met Gly 120 125 130 atg gtc tat gaa gcc cgc cct aac gtc acc gtc gac gcc ttc ggc ctg 547 Met Val Tyr Glu Ala Arg Pro Asn Val Thr Val Asp Ala Phe Gly Leu 135 140 145 gca ctc aag tcc gga aac gta gct ttg ctg cgc ggt tcc tcc aca gct 595 Ala Leu Lys Ser Gly Asn Val Ala Leu Leu Arg Gly Ser Ser Thr Ala 150 155 160 165 gtg cat tcc aac acc aag ctc gtg gaa atc ctg cag gac gtc ctc gag 643 Val His Ser Asn Thr Lys Leu Val Glu Ile Leu Gln Asp Val Leu Glu 170 175 180 cgt ttc gag ctg cca cgc gaa acc gtg cag ttg ctg cct tgc caa acc 691 Arg Phe Glu Leu Pro Arg Glu Thr Val Gln Leu Leu Pro Cys Gln Thr 185 190 195 cgc gga tcc gtc caa gat ttg atc acc gca cgc ggc ctc gtt gac gtg 739 Arg Gly Ser Val Gln Asp Leu Ile Thr Ala Arg Gly Leu Val Asp Val 200 205 210 gtc atc cca cgc ggc ggc gca gga cta atc aac gca gtg gtc acc ggt 787 Val Ile Pro Arg Gly Gly Ala Gly Leu Ile Asn Ala Val Val Thr Gly 215 220 225 gcg acc gtg ccc acc att gaa acc ggc acc ggc aac tgc cac ttc tac 835 Ala Thr Val Pro Thr Ile Glu Thr Gly Thr Gly Asn Cys His Phe Tyr 230 235 240 245 atc gat gcc gaa gcc aag ctt gat cag gca atc gcc atg gtc atc aac 883 Ile Asp Ala Glu Ala Lys Leu Asp Gln Ala Ile Ala Met Val Ile Asn 250 255 260 ggc aag acc cgc cgc tgc agc gtg tgc aac gct act gaa acc gcg ctt 931 Gly Lys Thr Arg Arg Cys Ser Val Cys Asn Ala Thr Glu Thr Ala Leu 265 270 275 ctc gac gcc gcc ctc agc gac tca gac aag ctt gca gtc gtc cag gcg 979 Leu Asp Ala Ala Leu Ser Asp Ser Asp Lys Leu Ala Val Val Gln Ala 280 285 290 ctc cag gaa gca gga gtc aca att cat gga cgg gtg gcc gaa ttg gaa 1027 Leu Gln Glu Ala Gly Val Thr Ile His Gly Arg Val Ala Glu Leu Glu 295 300 305 gca ttc ggt gca acc gac gtg gtg gaa gca act gaa act gac tgg gat 1075 Ala Phe Gly Ala Thr Asp Val Val Glu Ala Thr Glu Thr Asp Trp Asp 310 315 320 325 tct gag tac ctg tcc ttc gat atc gct gtc gct gtg gtt gac ggt gtg 1123 Ser Glu Tyr Leu Ser Phe Asp Ile Ala Val Ala Val Val Asp Gly Val 330 335 340 gat gga gct ctg gca cac atc gct aag tac agc acc aag cac acc gaa 1171 Asp Gly Ala Leu Ala His Ile Ala Lys Tyr Ser Thr Lys His Thr Glu 345 350 355 gcg atc gcc acc caa aac att gaa acc gct cag cgc ttt gca gat cgc 1219 Ala Ile Ala Thr Gln Asn Ile Glu Thr Ala Gln Arg Phe Ala Asp Arg 360 365 370 gtc gat gca gca gcg gtg atg ata aac gca tcc acc gcc tac acc gat 1267 Val Asp Ala Ala Ala Val Met Ile Asn Ala Ser Thr Ala Tyr Thr Asp 375 380 385 ggg gag cag tac ggc atg ggc gcg gag atc ggc att tcc acc cag aaa 1315 Gly Glu Gln Tyr Gly Met Gly Ala Glu Ile Gly Ile Ser Thr Gln Lys 390 395 400 405 ctg cat gca cgt gga cca atg gcc ctg cca gag ctg acc tcc acc aag 1363 Leu His Ala Arg Gly Pro Met Ala Leu Pro Glu Leu Thr Ser Thr Lys 410 415 420 tgg att ctg cag ggc aca gga caa att agg cct taagtttgaa gaagtaatca 1416 Trp Ile Leu Gln Gly Thr Gly Gln Ile Arg Pro 425 430 agctcccctg 1426 <210> 106 <211> 432 <212> PRT <213> Corynebacterium glutamicum <400> 106 Met Ser Ser Thr Thr Leu Thr Asp Asp Gln Ile Arg Asp Asn Glu Arg 1 5 10 15 Thr Glu Val Leu Ala Lys Ala Thr Ala Ala Lys Asn Ile Val Pro Asp 20 25 30 Ile Ala Val Leu Gly Thr Gly Pro Lys Asn Ala Ile Leu Arg Ala Ala 35 40 45 Ala Asp Glu Leu Val Ala Arg Ser Ala Glu Ile Ile Glu Ala Asn Ala 50 55 60 Ser Asp Ile Glu Ala Gly Arg Ala Asn Gly Met Glu Glu Ser Met Ile 65 70 75 80 Asp Arg Leu Ala Leu Asp Glu Ser Arg Ile Glu Gly Ile Ala Gly Gly 85 90 95 Leu Arg Gln Val Ala Gly Leu Thr Asp Pro Val Gly Glu Val Leu Arg 100 105 110 Gly His Val Met Glu Asn Gly Ile Gln Met Lys Gln Val Arg Val Pro 115 120 125 Leu Gly Val Met Gly Met Val Tyr Glu Ala Arg Pro Asn Val Thr Val 130 135 140 Asp Ala Phe Gly Leu Ala Leu Lys Ser Gly Asn Val Ala Leu Leu Arg 145 150 155 160 Gly Ser Ser Thr Ala Val His Ser Asn Thr Lys Leu Val Glu Ile Leu 165 170 175 Gln Asp Val Leu Glu Arg Phe Glu Leu Pro Arg Glu Thr Val Gln Leu 180 185 190 Leu Pro Cys Gln Thr Arg Gly Ser Val Gln Asp Leu Ile Thr Ala Arg 195 200 205 Gly Leu Val Asp Val Val Ile Pro Arg Gly Gly Ala Gly Leu Ile Asn 210 215 220 Ala Val Val Thr Gly Ala Thr Val Pro Thr Ile Glu Thr Gly Thr Gly 225 230 235 240 Asn Cys His Phe Tyr Ile Asp Ala Glu Ala Lys Leu Asp Gln Ala Ile 245 250 255 Ala Met Val Ile Asn Gly Lys Thr Arg Arg Cys Ser Val Cys Asn Ala 260 265 270 Thr Glu Thr Ala Leu Leu Asp Ala Ala Leu Ser Asp Ser Asp Lys Leu 275 280 285 Ala Val Val Gln Ala Leu Gln Glu Ala Gly Val Thr Ile His Gly Arg 290 295 300 Val Ala Glu Leu Glu Ala Phe Gly Ala Thr Asp Val Val Glu Ala Thr 305 310 315 320 Glu Thr Asp Trp Asp Ser Glu Tyr Leu Ser Phe Asp Ile Ala Val Ala 325 330 335 Val Val Asp Gly Val Asp Gly Ala Leu Ala His Ile Ala Lys Tyr Ser 340 345 350 Thr Lys His Thr Glu Ala Ile Ala Thr Gln Asn Ile Glu Thr Ala Gln 355 360 365 Arg Phe Ala Asp Arg Val Asp Ala Ala Ala Val Met Ile Asn Ala Ser 370 375 380 Thr Ala Tyr Thr Asp Gly Glu Gln Tyr Gly Met Gly Ala Glu Ile Gly 385 390 395 400 Ile Ser Thr Gln Lys Leu His Ala Arg Gly Pro Met Ala Leu Pro Glu 405 410 415 Leu Thr Ser Thr Lys Trp Ile Leu Gln Gly Thr Gly Gln Ile Arg Pro 420 425 430 <210> 107 <211> 799 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(769) <223> RXA02390 <400> 107 gctggtggtg ctgacccata cgctggaact ccaactgctg ttgataccgc caagatgttt 60 ggccgcgagg atctcgtagc tcgcttcgag tcataggccg gtg gag tgg acc gct 115 Val Glu Trp Thr Ala 1 5 ttt ggc acc ctg att ctg ctc aat ttg gtg ggc agt tta tcc ccg ggg 163 Phe Gly Thr Leu Ile Leu Leu Asn Leu Val Gly Ser Leu Ser Pro Gly 10 15 20 cct gat acc ttt ttc ctc ctc cgc tta gcc acc cgc tcc aga gcg cac 211 Pro Asp Thr Phe Phe Leu Leu Arg Leu Ala Thr Arg Ser Arg Ala His 25 30 35 gcg atc gct ggc gtc gcc ggc atc gtc acc gga ctc acg gtg tgg gtg 259 Ala Ile Ala Gly Val Ala Gly Ile Val Thr Gly Leu Thr Val Trp Val 40 45 50 acg ctg acg gtc gtg gga gca gcg gcg ctg ctc acc act tat ccg tcg 307 Thr Leu Thr Val Val Gly Ala Ala Ala Leu Leu Thr Thr Tyr Pro Ser 55 60 65 att ctc gga atc atc cag ctc gtc ggc ggc acg tac cta agc ttc att 355 Ile Leu Gly Ile Ile Gln Leu Val Gly Gly Thr Tyr Leu Ser Phe Ile 70 75 80 85 ggg tac aag ttg ctg cgc tcg gcg tcg aga gag ctt atc gac gcc cgc 403 Gly Tyr Lys Leu Leu Arg Ser Ala Ser Arg Glu Leu Ile Asp Ala Arg 90 95 100 cag ttc cgt ttc aac gcc gat gcc cga cct atc ccg gat gcg gta gaa 451 Gln Phe Arg Phe Asn Ala Asp Ala Arg Pro Ile Pro Asp Ala Val Glu 105 110 115 gca ctg gga acc cgc act cag gta tat cga caa ggt ttg gcc acc aac 499 Ala Leu Gly Thr Arg Thr Gln Val Tyr Arg Gln Gly Leu Ala Thr Asn 120 125 130 ctg tca aac cct aaa gtt gtc atg tac ttc gcg gca att ctg gct ccg 547 Leu Ser Asn Pro Lys Val Val Met Tyr Phe Ala Ala Ile Leu Ala Pro 135 140 145 ttg atg cca gcg cac cca tca ccg gtg ctg gcg ttc tct atc atc gtg 595 Leu Met Pro Ala His Pro Ser Pro Val Leu Ala Phe Ser Ile Ile Val 150 155 160 165 gcg att tta gtg cag acc ttt gtt acc ttc tct gct gtg tgc ctc att 643 Ala Ile Leu Val Gln Thr Phe Val Thr Phe Ser Ala Val Cys Leu Ile 170 175 180 gtc tct acg gag cgt gtg cgc aaa gca atg ctg cgt gca ggt ccc tgg 691 Val Ser Thr Glu Arg Val Arg Lys Ala Met Leu Arg Ala Gly Pro Trp 185 190 195 ttt gac ctg ctt gct ggc gtt gtc ttc ctc gtt gtg ggt gtg act ctg 739 Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val Val Gly Val Thr Leu 200 205 210 ctg tat gaa ggc ctg acc ggt tta ctc ggg taaaggcata aaaaatggct 789 Leu Tyr Glu Gly Leu Thr Gly Leu Leu Gly 215 220 tccaatcaaa 799 <210> 108 <211> 223 <212> PRT <213> Corynebacterium glutamicum <400> 108 Val Glu Trp Thr Ala Phe Gly Thr Leu Ile Leu Leu Asn Leu Val Gly 1 5 10 15 Ser Leu Ser Pro Gly Pro Asp Thr Phe Phe Leu Leu Arg Leu Ala Thr 20 25 30 Arg Ser Arg Ala His Ala Ile Ala Gly Val Ala Gly Ile Val Thr Gly 35 40 45 Leu Thr Val Trp Val Thr Leu Thr Val Val Gly Ala Ala Ala Leu Leu 50 55 60 Thr Thr Tyr Pro Ser Ile Leu Gly Ile Ile Gln Leu Val Gly Gly Thr 65 70 75 80 Tyr Leu Ser Phe Ile Gly Tyr Lys Leu Leu Arg Ser Ala Ser Arg Glu 85 90 95 Leu Ile Asp Ala Arg Gln Phe Arg Phe Asn Ala Asp Ala Arg Pro Ile 100 105 110 Pro Asp Ala Val Glu Ala Leu Gly Thr Arg Thr Gln Val Tyr Arg Gln 115 120 125 Gly Leu Ala Thr Asn Leu Ser Asn Pro Lys Val Val Met Tyr Phe Ala 130 135 140 Ala Ile Leu Ala Pro Leu Met Pro Ala His Pro Ser Pro Val Leu Ala 145 150 155 160 Phe Ser Ile Ile Val Ala Ile Leu Val Gln Thr Phe Val Thr Phe Ser 165 170 175 Ala Val Cys Leu Ile Val Ser Thr Glu Arg Val Arg Lys Ala Met Leu 180 185 190 Arg Ala Gly Pro Trp Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val 195 200 205 Val Gly Val Thr Leu Leu Tyr Glu Gly Leu Thr Gly Leu Leu Gly 210 215 220 <210> 109 <211> 991 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(961) <223> RXA02499 <400> 109 tttgcgcacc tcacgtgatg gtttcagcat ggttctcgca ggaatcgtgg gcctggtcat 60 gacgttcggg ccgctggcaa tcgtcatgta atttgtcgtt ttg ggc ccc cgc tgc 115 Leu Gly Pro Arg Cys 1 5 tgc aaa aca ggg gtg gtt agt ggc agt gtg gga acc atg aca aca att 163 Cys Lys Thr Gly Val Val Ser Gly Ser Val Gly Thr Met Thr Thr Ile 10 15 20 gct gta atc ggc ggc gga caa atc ggc gag gct tta gtc tca ggt ttg 211 Ala Val Ile Gly Gly Gly Gln Ile Gly Glu Ala Leu Val Ser Gly Leu 25 30 35 atc gcg gcc aac atg aat cca caa aat att cgc gtc acc aac cgt tcg 259 Ile Ala Ala Asn Met Asn Pro Gln Asn Ile Arg Val Thr Asn Arg Ser 40 45 50 gaa gag cgc ggc caa gag ctg cgt gac cgc tac ggc atc ctc aac atg 307 Glu Glu Arg Gly Gln Glu Leu Arg Asp Arg Tyr Gly Ile Leu Asn Met 55 60 65 acg gat aat tcc caa gcc gca gac gaa gcc gac gtg gtg ttc ctg tgc 355 Thr Asp Asn Ser Gln Ala Ala Asp Glu Ala Asp Val Val Phe Leu Cys 70 75 80 85 gtg aag ccg aaa ttt atc gtc gaa gtg ctc tcc gaa atc acc ggc act 403 Val Lys Pro Lys Phe Ile Val Glu Val Leu Ser Glu Ile Thr Gly Thr 90 95 100 ttg gat aac aac tcc gca caa agt gtt gtg gtc agc atg gcc gca ggc 451 Leu Asp Asn Asn Ser Ala Gln Ser Val Val Val Ser Met Ala Ala Gly 105 110 115 atc agc atc gct gcc atg gaa gaa agc gcc tct gcg ggg ctc ccc gtc 499 Ile Ser Ile Ala Ala Met Glu Glu Ser Ala Ser Ala Gly Leu Pro Val 120 125 130 gtg cgc gtc atg ccg aac act cca atg ctc gtg ggc aag ggc atg tcg 547 Val Arg Val Met Pro Asn Thr Pro Met Leu Val Gly Lys Gly Met Ser 135 140 145 act gtc acc aaa ggc cgc tac gtt gac gcg gaa cag ttg gaa caa gtc 595 Thr Val Thr Lys Gly Arg Tyr Val Asp Ala Glu Gln Leu Glu Gln Val 150 155 160 165 aag gac ttg ttg agc acc gtt gga gac gtc ctc gaa gtc gcg gaa tca 643 Lys Asp Leu Leu Ser Thr Val Gly Asp Val Leu Glu Val Ala Glu Ser 170 175 180 gac atc gac gca gtc acc gcg atg tcc gga tcc tcc cct gca tac ctg 691 Asp Ile Asp Ala Val Thr Ala Met Ser Gly Ser Ser Pro Ala Tyr Leu 185 190 195 ttc ctt gtg acc gaa gcg ctc att gag gca gga gtt aat cta ggc ctg 739 Phe Leu Val Thr Glu Ala Leu Ile Glu Ala Gly Val Asn Leu Gly Leu 200 205 210 ccc cgc gcg acc gct aaa aag ctc gct gtg gcc tca ttc gaa ggt gct 787 Pro Arg Ala Thr Ala Lys Lys Leu Ala Val Ala Ser Phe Glu Gly Ala 215 220 225 gca acc atg atg aag gaa acc ggc aaa gaa ccc tca gaa ttg cgc gca 835 Ala Thr Met Met Lys Glu Thr Gly Lys Glu Pro Ser Glu Leu Arg Ala 230 235 240 245 ggc gtt tcc tca ccc gca ggc acc acc gtc gca gcc atc cga gaa ctc 883 Gly Val Ser Ser Pro Ala Gly Thr Thr Val Ala Ala Ile Arg Glu Leu 250 255 260 gaa gaa agc gga atc cga ggc gct ttc tac cgc gca gcc caa gct tgc 931 Glu Glu Ser Gly Ile Arg Gly Ala Phe Tyr Arg Ala Ala Gln Ala Cys 265 270 275 gcc gac cga tct gaa gaa ctc gga aag cgc tagaaaccgt tatttccccg 981 Ala Asp Arg Ser Glu Glu Leu Gly Lys Arg 280 285 ttatcagctc 991 <210> 110 <211> 287 <212> PRT <213> Corynebacterium glutamicum <400> 110 Leu Gly Pro Arg Cys Cys Lys Thr Gly Val Val Ser Gly Ser Val Gly 1 5 10 15 Thr Met Thr Thr Ile Ala Val Ile Gly Gly Gly Gln Ile Gly Glu Ala 20 25 30 Leu Val Ser Gly Leu Ile Ala Ala Asn Met Asn Pro Gln Asn Ile Arg 35 40 45 Val Thr Asn Arg Ser Glu Glu Arg Gly Gln Glu Leu Arg Asp Arg Tyr 50 55 60 Gly Ile Leu Asn Met Thr Asp Asn Ser Gln Ala Ala Asp Glu Ala Asp 65 70 75 80 Val Val Phe Leu Cys Val Lys Pro Lys Phe Ile Val Glu Val Leu Ser 85 90 95 Glu Ile Thr Gly Thr Leu Asp Asn Asn Ser Ala Gln Ser Val Val Val 100 105 110 Ser Met Ala Ala Gly Ile Ser Ile Ala Ala Met Glu Glu Ser Ala Ser 115 120 125 Ala Gly Leu Pro Val Val Arg Val Met Pro Asn Thr Pro Met Leu Val 130 135 140 Gly Lys Gly Met Ser Thr Val Thr Lys Gly Arg Tyr Val Asp Ala Glu 145 150 155 160 Gln Leu Glu Gln Val Lys Asp Leu Leu Ser Thr Val Gly Asp Val Leu 165 170 175 Glu Val Ala Glu Ser Asp Ile Asp Ala Val Thr Ala Met Ser Gly Ser 180 185 190 Ser Pro Ala Tyr Leu Phe Leu Val Thr Glu Ala Leu Ile Glu Ala Gly 195 200 205 Val Asn Leu Gly Leu Pro Arg Ala Thr Ala Lys Lys Leu Ala Val Ala 210 215 220 Ser Phe Glu Gly Ala Ala Thr Met Met Lys Glu Thr Gly Lys Glu Pro 225 230 235 240 Ser Glu Leu Arg Ala Gly Val Ser Ser Pro Ala Gly Thr Thr Val Ala 245 250 255 Ala Ile Arg Glu Leu Glu Glu Ser Gly Ile Arg Gly Ala Phe Tyr Arg 260 265 270 Ala Ala Gln Ala Cys Ala Asp Arg Ser Glu Glu Leu Gly Lys Arg 275 280 285 <210> 111 <211> 1441 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1411) <223> RXA02516 <400> 111 ttgatgatca cccactacaa gcgcatcctc aactacgtta agcctgactt cattcacgtt 60 ttcgcgaatg gccagattgt gaccaccggt ggcgctgagc ttg ctg aca agc tcg 115 Leu Leu Thr Ser Ser 1 5 agg ctg acg gct acg acc agt tca tca agt aac atg tcc gat ttc ctc 163 Arg Leu Thr Ala Thr Thr Ser Ser Ser Ser Asn Met Ser Asp Phe Leu 10 15 20 aat gca gat gga tcc ctc aat gtg gat aag gtg cgg gaa gaa ttc cca 211 Asn Ala Asp Gly Ser Leu Asn Val Asp Lys Val Arg Glu Glu Phe Pro 25 30 35 atc ctg aag cgc act gtt agg gat ggg aaa ccg ctt gct tac ctg gac 259 Ile Leu Lys Arg Thr Val Arg Asp Gly Lys Pro Leu Ala Tyr Leu Asp 40 45 50 tca ggt gcg aca tcg cag cga ccc gag cgg gtg tgg cgt gca gag gag 307 Ser Gly Ala Thr Ser Gln Arg Pro Glu Arg Val Trp Arg Ala Glu Glu 55 60 65 cac ttt gtg ctg cac acc aac gcc ccc gtg cac cgc ggt gcc tac caa 355 His Phe Val Leu His Thr Asn Ala Pro Val His Arg Gly Ala Tyr Gln 70 75 80 85 ctg gct gag gaa gca acg gat gct tat gaa ggt gcc cgc gag aag atc 403 Leu Ala Glu Glu Ala Thr Asp Ala Tyr Glu Gly Ala Arg Glu Lys Ile 90 95 100 gct gcc ttt gtt ggt gcc gag cag cat gaa att gcg ttc act aag aat 451 Ala Ala Phe Val Gly Ala Glu Gln His Glu Ile Ala Phe Thr Lys Asn 105 110 115 gca act gaa gca ctc aat ctt gtt gcg tac acc ttg ggt gat gac cgt 499 Ala Thr Glu Ala Leu Asn Leu Val Ala Tyr Thr Leu Gly Asp Asp Arg 120 125 130 tcc ggt aag tat cgt gtc cag gcc ggg gat acc gtg gtc atc acg gag 547 Ser Gly Lys Tyr Arg Val Gln Ala Gly Asp Thr Val Val Ile Thr Glu 135 140 145 cta gag cac cac gca aac ttg gtg cca tgg cag gag ctg tgc cgt cga 595 Leu Glu His His Ala Asn Leu Val Pro Trp Gln Glu Leu Cys Arg Arg 150 155 160 165 acc ggt gcg aca ttg aag tgg tac aag gtg act gaa gat ggt cgc att 643 Thr Gly Ala Thr Leu Lys Trp Tyr Lys Val Thr Glu Asp Gly Arg Ile 170 175 180 gat ctc gat tca ctc gag ctt gat gaa act gtc aag gtc gtt gcc ttc 691 Asp Leu Asp Ser Leu Glu Leu Asp Glu Thr Val Lys Val Val Ala Phe 185 190 195 act cac cag tcc aat gtg acc ggt gct gtg gct gat gtt cca gag ttg 739 Thr His Gln Ser Asn Val Thr Gly Ala Val Ala Asp Val Pro Glu Leu 200 205 210 gtt cgt cgt gcc aag gct gtc ggc gct ctc acg gtg ctt gat gcg tgc 787 Val Arg Arg Ala Lys Ala Val Gly Ala Leu Thr Val Leu Asp Ala Cys 215 220 225 cag tct gtt cct cat atg cca gtg aat ttc cac gag ctg gat gta gat 835 Gln Ser Val Pro His Met Pro Val Asn Phe His Glu Leu Asp Val Asp 230 235 240 245 ttc tct gca ttc tct ggc cat aag atg ctg gga cct gca ggc gtg ggc 883 Phe Ser Ala Phe Ser Gly His Lys Met Leu Gly Pro Ala Gly Val Gly 250 255 260 gtt gtg tat gca aag tcc cca atc ttg gat gaa ctg cca cca ttt ttg 931 Val Val Tyr Ala Lys Ser Pro Ile Leu Asp Glu Leu Pro Pro Phe Leu 265 270 275 act ggt ggt tcc atg att gaa gtt gtc acc atg gag ggt tcc acc tac 979 Thr Gly Gly Ser Met Ile Glu Val Val Thr Met Glu Gly Ser Thr Tyr 280 285 290 gct gcc gca cct caa cgt ttt gag gcc ggc acg cag atg acc agc cag 1027 Ala Ala Ala Pro Gln Arg Phe Glu Ala Gly Thr Gln Met Thr Ser Gln 295 300 305 gtt gtg ggc ttg ggt gct gcc gtg gac atg ctg aat gaa atc ggt atg 1075 Val Val Gly Leu Gly Ala Ala Val Asp Met Leu Asn Glu Ile Gly Met 310 315 320 325 gaa gca atc gca gcg cat gag cac gca ttg act gct tac gcg ttg gaa 1123 Glu Ala Ile Ala Ala His Glu His Ala Leu Thr Ala Tyr Ala Leu Glu 330 335 340 aag ctc acg gca att aag gga cta acc att gct ggt cct ttg act gca 1171 Lys Leu Thr Ala Ile Lys Gly Leu Thr Ile Ala Gly Pro Leu Thr Ala 345 350 355 gag cag cgc ggc ggt gca atc agc ttc ggt gtc gag ggc att cac cca 1219 Glu Gln Arg Gly Gly Ala Ile Ser Phe Gly Val Glu Gly Ile His Pro 360 365 370 cac gat cta ggc caa gtg ctt gac gat cag ggc gtg aat atc cgc gtc 1267 His Asp Leu Gly Gln Val Leu Asp Asp Gln Gly Val Asn Ile Arg Val 375 380 385 ggc cac cac tgc gcg tgg ccc gtg cac cgc agc atg aac gta caa tcg 1315 Gly His His Cys Ala Trp Pro Val His Arg Ser Met Asn Val Gln Ser 390 395 400 405 aca gca aga gca tct ttc tat ctc tat aac acc ttc gaa gaa atc gac 1363 Thr Ala Arg Ala Ser Phe Tyr Leu Tyr Asn Thr Phe Glu Glu Ile Asp 410 415 420 cgc ctc gcg gca gcg atc gag aag gcc aag caa ttc ttt gga gtt gag 1411 Arg Leu Ala Ala Ala Ile Glu Lys Ala Lys Gln Phe Phe Gly Val Glu 425 430 435 taatgaacct tgagcagatg taccaggagg 1441 <210> 112 <211> 437 <212> PRT <213> Corynebacterium glutamicum <400> 112 Leu Leu Thr Ser Ser Arg Leu Thr Ala Thr Thr Ser Ser Ser Ser Asn 1 5 10 15 Met Ser Asp Phe Leu Asn Ala Asp Gly Ser Leu Asn Val Asp Lys Val 20 25 30 Arg Glu Glu Phe Pro Ile Leu Lys Arg Thr Val Arg Asp Gly Lys Pro 35 40 45 Leu Ala Tyr Leu Asp Ser Gly Ala Thr Ser Gln Arg Pro Glu Arg Val 50 55 60 Trp Arg Ala Glu Glu His Phe Val Leu His Thr Asn Ala Pro Val His 65 70 75 80 Arg Gly Ala Tyr Gln Leu Ala Glu Glu Ala Thr Asp Ala Tyr Glu Gly 85 90 95 Ala Arg Glu Lys Ile Ala Ala Phe Val Gly Ala Glu Gln His Glu Ile 100 105 110 Ala Phe Thr Lys Asn Ala Thr Glu Ala Leu Asn Leu Val Ala Tyr Thr 115 120 125 Leu Gly Asp Asp Arg Ser Gly Lys Tyr Arg Val Gln Ala Gly Asp Thr 130 135 140 Val Val Ile Thr Glu Leu Glu His His Ala Asn Leu Val Pro Trp Gln 145 150 155 160 Glu Leu Cys Arg Arg Thr Gly Ala Thr Leu Lys Trp Tyr Lys Val Thr 165 170 175 Glu Asp Gly Arg Ile Asp Leu Asp Ser Leu Glu Leu Asp Glu Thr Val 180 185 190 Lys Val Val Ala Phe Thr His Gln Ser Asn Val Thr Gly Ala Val Ala 195 200 205 Asp Val Pro Glu Leu Val Arg Arg Ala Lys Ala Val Gly Ala Leu Thr 210 215 220 Val Leu Asp Ala Cys Gln Ser Val Pro His Met Pro Val Asn Phe His 225 230 235 240 Glu Leu Asp Val Asp Phe Ser Ala Phe Ser Gly His Lys Met Leu Gly 245 250 255 Pro Ala Gly Val Gly Val Val Tyr Ala Lys Ser Pro Ile Leu Asp Glu 260 265 270 Leu Pro Pro Phe Leu Thr Gly Gly Ser Met Ile Glu Val Val Thr Met 275 280 285 Glu Gly Ser Thr Tyr Ala Ala Ala Pro Gln Arg Phe Glu Ala Gly Thr 290 295 300 Gln Met Thr Ser Gln Val Val Gly Leu Gly Ala Ala Val Asp Met Leu 305 310 315 320 Asn Glu Ile Gly Met Glu Ala Ile Ala Ala His Glu His Ala Leu Thr 325 330 335 Ala Tyr Ala Leu Glu Lys Leu Thr Ala Ile Lys Gly Leu Thr Ile Ala 340 345 350 Gly Pro Leu Thr Ala Glu Gln Arg Gly Gly Ala Ile Ser Phe Gly Val 355 360 365 Glu Gly Ile His Pro His Asp Leu Gly Gln Val Leu Asp Asp Gln Gly 370 375 380 Val Asn Ile Arg Val Gly His His Cys Ala Trp Pro Val His Arg Ser 385 390 395 400 Met Asn Val Gln Ser Thr Ala Arg Ala Ser Phe Tyr Leu Tyr Asn Thr 405 410 415 Phe Glu Glu Ile Asp Arg Leu Ala Ala Ala Ile Glu Lys Ala Lys Gln 420 425 430 Phe Phe Gly Val Glu 435 <210> 113 <211> 1276 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1246) <223> RXA02532 <400> 113 agataccgac tttgctaatc ccaccaccgt agccactagg tatttagttg ccttggacaa 60 gcgtgtacct cgtgcttgat ataacaagta aggaagcctg atg aat ttt tac cca 115 Met Asn Phe Tyr Pro 1 5 cca tct gta cct att aac cct gcg tgg cgt cca ccc aca gta act gtg 163 Pro Ser Val Pro Ile Asn Pro Ala Trp Arg Pro Pro Thr Val Thr Val 10 15 20 caa gcg gga cgg cca gcc aga act cct ggt gcg ccg atg aac cca cct 211 Gln Ala Gly Arg Pro Ala Arg Thr Pro Gly Ala Pro Met Asn Pro Pro 25 30 35 atc acg ttg tcc agc act tat gtt cat gat tca gaa aaa gct tat ggg 259 Ile Thr Leu Ser Ser Thr Tyr Val His Asp Ser Glu Lys Ala Tyr Gly 40 45 50 cgc gat ggc aat gat gga tgg ggt gca ttt gag gct gcc atg gga act 307 Arg Asp Gly Asn Asp Gly Trp Gly Ala Phe Glu Ala Ala Met Gly Thr 55 60 65 cta gat ggt ggg ttc gcg gta tct tat tct tca ggt ttg gca gcg gca 355 Leu Asp Gly Gly Phe Ala Val Ser Tyr Ser Ser Gly Leu Ala Ala Ala 70 75 80 85 acg tcg att gct gat ttg gtt cct act ggt ggc aca gtt gtt tta cct 403 Thr Ser Ile Ala Asp Leu Val Pro Thr Gly Gly Thr Val Val Leu Pro 90 95 100 aaa gct gcc tat tat ggc gtg acc aat att ttc gcc agg atg gaa gcc 451 Lys Ala Ala Tyr Tyr Gly Val Thr Asn Ile Phe Ala Arg Met Glu Ala 105 110 115 cgc gga agg ctg aag gtt cga act gtt gat gca gac aat acc gaa gaa 499 Arg Gly Arg Leu Lys Val Arg Thr Val Asp Ala Asp Asn Thr Glu Glu 120 125 130 gtg att gct gct gct caa ggt gca gat gtg gtg tgg gtg gaa tcg atc 547 Val Ile Ala Ala Ala Gln Gly Ala Asp Val Val Trp Val Glu Ser Ile 135 140 145 gct aat ccg acg atg gtg gta gct gat atc cct gca ata gtc gac ggt 595 Ala Asn Pro Thr Met Val Val Ala Asp Ile Pro Ala Ile Val Asp Gly 150 155 160 165 gtg cgt ggg ctt gga gtt ttg act gtc gtt gac gcg act ttc gca acg 643 Val Arg Gly Leu Gly Val Leu Thr Val Val Asp Ala Thr Phe Ala Thr 170 175 180 cca ctt cgt caa cgt cca ttg gaa ctt ggt gct gat att gtg ctt tac 691 Pro Leu Arg Gln Arg Pro Leu Glu Leu Gly Ala Asp Ile Val Leu Tyr 185 190 195 tcg gca acc aaa ctt atc ggt gga cac tct gat ctt ctt ctt gga gtc 739 Ser Ala Thr Lys Leu Ile Gly Gly His Ser Asp Leu Leu Leu Gly Val 200 205 210 gca gtg tgc aag tct gag cac cat gcg cag ttt ctt gcc act cac cgt 787 Ala Val Cys Lys Ser Glu His His Ala Gln Phe Leu Ala Thr His Arg 215 220 225 cat gat cat ggt tca gtg ccg gga ggt ctt gaa gcg ttt ctt gct ctc 835 His Asp His Gly Ser Val Pro Gly Gly Leu Glu Ala Phe Leu Ala Leu 230 235 240 245 cgt gga ttg tat tcc ttg gcg gtg cgt ctt gat cga gca gaa tcc aac 883 Arg Gly Leu Tyr Ser Leu Ala Val Arg Leu Asp Arg Ala Glu Ser Asn 250 255 260 gca gca gaa ctt tcg cgg cga ctt aac gcg cat cct tcg gtt acc cgc 931 Ala Ala Glu Leu Ser Arg Arg Leu Asn Ala His Pro Ser Val Thr Arg 265 270 275 gtc aat tat cca gga ctt cct gat gat ccc caa cat gaa aaa gcc gtg 979 Val Asn Tyr Pro Gly Leu Pro Asp Asp Pro Gln His Glu Lys Ala Val 280 285 290 cga gtc cta ccc tct gga tgt gga aac atg ttg tca ttt gag ctt gat 1027 Arg Val Leu Pro Ser Gly Cys Gly Asn Met Leu Ser Phe Glu Leu Asp 295 300 305 gca aca cct gaa cga act gat gag att ctc gaa agc ctg tca ctt tta 1075 Ala Thr Pro Glu Arg Thr Asp Glu Ile Leu Glu Ser Leu Ser Leu Leu 310 315 320 325 acc cac gcg acc agt tgg gga ggt gtg gaa aca gcc att gaa cgt cgc 1123 Thr His Ala Thr Ser Trp Gly Gly Val Glu Thr Ala Ile Glu Arg Arg 330 335 340 acc agg cgg gat gct gaa gtg gtg gca gga gta ccg atg act ctt tgc 1171 Thr Arg Arg Asp Ala Glu Val Val Ala Gly Val Pro Met Thr Leu Cys 345 350 355 cgc gtt tcc gta gga att gaa gac gtt gaa gat cta tgg gaa gac ctc 1219 Arg Val Ser Val Gly Ile Glu Asp Val Glu Asp Leu Trp Glu Asp Leu 360 365 370 aac gcc tca atc gac aaa gtt ctg ggt tagaactcgt agccagtaac 1266 Asn Ala Ser Ile Asp Lys Val Leu Gly 375 380 cagaccttca 1276 <210> 114 <211> 382 <212> PRT <213> Corynebacterium glutamicum <400> 114 Met Asn Phe Tyr Pro Pro Ser Val Pro Ile Asn Pro Ala Trp Arg Pro 1 5 10 15 Pro Thr Val Thr Val Gln Ala Gly Arg Pro Ala Arg Thr Pro Gly Ala 20 25 30 Pro Met Asn Pro Pro Ile Thr Leu Ser Ser Thr Tyr Val His Asp Ser 35 40 45 Glu Lys Ala Tyr Gly Arg Asp Gly Asn Asp Gly Trp Gly Ala Phe Glu 50 55 60 Ala Ala Met Gly Thr Leu Asp Gly Gly Phe Ala Val Ser Tyr Ser Ser 65 70 75 80 Gly Leu Ala Ala Ala Thr Ser Ile Ala Asp Leu Val Pro Thr Gly Gly 85 90 95 Thr Val Val Leu Pro Lys Ala Ala Tyr Tyr Gly Val Thr Asn Ile Phe 100 105 110 Ala Arg Met Glu Ala Arg Gly Arg Leu Lys Val Arg Thr Val Asp Ala 115 120 125 Asp Asn Thr Glu Glu Val Ile Ala Ala Ala Gln Gly Ala Asp Val Val 130 135 140 Trp Val Glu Ser Ile Ala Asn Pro Thr Met Val Val Ala Asp Ile Pro 145 150 155 160 Ala Ile Val Asp Gly Val Arg Gly Leu Gly Val Leu Thr Val Val Asp 165 170 175 Ala Thr Phe Ala Thr Pro Leu Arg Gln Arg Pro Leu Glu Leu Gly Ala 180 185 190 Asp Ile Val Leu Tyr Ser Ala Thr Lys Leu Ile Gly Gly His Ser Asp 195 200 205 Leu Leu Leu Gly Val Ala Val Cys Lys Ser Glu His His Ala Gln Phe 210 215 220 Leu Ala Thr His Arg His Asp His Gly Ser Val Pro Gly Gly Leu Glu 225 230 235 240 Ala Phe Leu Ala Leu Arg Gly Leu Tyr Ser Leu Ala Val Arg Leu Asp 245 250 255 Arg Ala Glu Ser Asn Ala Ala Glu Leu Ser Arg Arg Leu Asn Ala His 260 265 270 Pro Ser Val Thr Arg Val Asn Tyr Pro Gly Leu Pro Asp Asp Pro Gln 275 280 285 His Glu Lys Ala Val Arg Val Leu Pro Ser Gly Cys Gly Asn Met Leu 290 295 300 Ser Phe Glu Leu Asp Ala Thr Pro Glu Arg Thr Asp Glu Ile Leu Glu 305 310 315 320 Ser Leu Ser Leu Leu Thr His Ala Thr Ser Trp Gly Gly Val Glu Thr 325 330 335 Ala Ile Glu Arg Arg Thr Arg Arg Asp Ala Glu Val Val Ala Gly Val 340 345 350 Pro Met Thr Leu Cys Arg Val Ser Val Gly Ile Glu Asp Val Glu Asp 355 360 365 Leu Trp Glu Asp Leu Asn Ala Ser Ile Asp Lys Val Leu Gly 370 375 380 <210> 115 <211> 928 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(898) <223> RXA02536 <400> 115 aggtacatgc tgctgattca agtggttttt acgatgctgg gcaagaagtg atcacgcgaa 60 cctgtgtata acttgcctca aagcgcctag gctgtggatt atg cgt att gcc ttg 115 Met Arg Ile Ala Leu 1 5 ctt cag atc tcg acg aat tcc gat aag atg gac aac ttc gcc ctg ctg 163 Leu Gln Ile Ser Thr Asn Ser Asp Lys Met Asp Asn Phe Ala Leu Leu 10 15 20 cgt gat gct gct gaa aaa gct gcg gaa cag ggg gct cgg gtg ttg gtg 211 Arg Asp Ala Ala Glu Lys Ala Ala Glu Gln Gly Ala Arg Val Leu Val 25 30 35 ttt ccg gag gcg act tcg caa agc ttt ggt acg gga agg ctt gat act 259 Phe Pro Glu Ala Thr Ser Gln Ser Phe Gly Thr Gly Arg Leu Asp Thr 40 45 50 cag gcg gag gag ctc gat ggc gaa ttc tcc acc gcg gta cga aaa tta 307 Gln Ala Glu Glu Leu Asp Gly Glu Phe Ser Thr Ala Val Arg Lys Leu 55 60 65 gcc gat gag ctg gac gtt gtc atc gtt gcg ggc atg ttc acc cct gct 355 Ala Asp Glu Leu Asp Val Val Ile Val Ala Gly Met Phe Thr Pro Ala 70 75 80 85 gac acc gtg cag cgc ggt gaa aaa acg atc tcg cgc gtc aac aac acc 403 Asp Thr Val Gln Arg Gly Glu Lys Thr Ile Ser Arg Val Asn Asn Thr 90 95 100 gtg ctg att agt ggc gct gga ttg cat cag gga tac aac aaa att cac 451 Val Leu Ile Ser Gly Ala Gly Leu His Gln Gly Tyr Asn Lys Ile His 105 110 115 aca tat gac gcg ttc ggt tat agg gaa tcc gac act gtg aaa ccg ggc 499 Thr Tyr Asp Ala Phe Gly Tyr Arg Glu Ser Asp Thr Val Lys Pro Gly 120 125 130 gat gag ctg gtt gta ttc gag gtc gac gat att aaa ttt ggt gtg gcg 547 Asp Glu Leu Val Val Phe Glu Val Asp Asp Ile Lys Phe Gly Val Ala 135 140 145 aca tgc tac gat att cga ttc cca gaa cag ttc aaa gac ctc gcc cgc 595 Thr Cys Tyr Asp Ile Arg Phe Pro Glu Gln Phe Lys Asp Leu Ala Arg 150 155 160 165 aac ggt gca cag ata att gtg gtt ccc acg tcg tgg caa gac ggt cct 643 Asn Gly Ala Gln Ile Ile Val Val Pro Thr Ser Trp Gln Asp Gly Pro 170 175 180 gga aaa tta gaa caa tgg gaa gtc ctc cct cgc gcg cgt gca ctg gat 691 Gly Lys Leu Glu Gln Trp Glu Val Leu Pro Arg Ala Arg Ala Leu Asp 185 190 195 tcc acc tgc tgg atc gta gcg tgt ggg caa gcg cga ctt cca gaa gaa 739 Ser Thr Cys Trp Ile Val Ala Cys Gly Gln Ala Arg Leu Pro Glu Glu 200 205 210 tta cgc gat gaa cga aaa ggc cct acg ggg att ggt cat tcc atg gtg 787 Leu Arg Asp Glu Arg Lys Gly Pro Thr Gly Ile Gly His Ser Met Val 215 220 225 aca aac cca cac ggt gaa gta att gct agc gcg ggt tat gag cca gaa 835 Thr Asn Pro His Gly Glu Val Ile Ala Ser Ala Gly Tyr Glu Pro Glu 230 235 240 245 atg ttg atc gcg gat att gat gtc agc ggt ttg gcc aaa att cgg gag 883 Met Leu Ile Ala Asp Ile Asp Val Ser Gly Leu Ala Lys Ile Arg Glu 250 255 260 gca ttg cct gtt ctt taaccactgt ctaaggaatc actcgttggg 928 Ala Leu Pro Val Leu 265 <210> 116 <211> 266 <212> PRT <213> Corynebacterium glutamicum <400> 116 Met Arg Ile Ala Leu Leu Gln Ile Ser Thr Asn Ser Asp Lys Met Asp 1 5 10 15 Asn Phe Ala Leu Leu Arg Asp Ala Ala Glu Lys Ala Ala Glu Gln Gly 20 25 30 Ala Arg Val Leu Val Phe Pro Glu Ala Thr Ser Gln Ser Phe Gly Thr 35 40 45 Gly Arg Leu Asp Thr Gln Ala Glu Glu Leu Asp Gly Glu Phe Ser Thr 50 55 60 Ala Val Arg Lys Leu Ala Asp Glu Leu Asp Val Val Ile Val Ala Gly 65 70 75 80 Met Phe Thr Pro Ala Asp Thr Val Gln Arg Gly Glu Lys Thr Ile Ser 85 90 95 Arg Val Asn Asn Thr Val Leu Ile Ser Gly Ala Gly Leu His Gln Gly 100 105 110 Tyr Asn Lys Ile His Thr Tyr Asp Ala Phe Gly Tyr Arg Glu Ser Asp 115 120 125 Thr Val Lys Pro Gly Asp Glu Leu Val Val Phe Glu Val Asp Asp Ile 130 135 140 Lys Phe Gly Val Ala Thr Cys Tyr Asp Ile Arg Phe Pro Glu Gln Phe 145 150 155 160 Lys Asp Leu Ala Arg Asn Gly Ala Gln Ile Ile Val Val Pro Thr Ser 165 170 175 Trp Gln Asp Gly Pro Gly Lys Leu Glu Gln Trp Glu Val Leu Pro Arg 180 185 190 Ala Arg Ala Leu Asp Ser Thr Cys Trp Ile Val Ala Cys Gly Gln Ala 195 200 205 Arg Leu Pro Glu Glu Leu Arg Asp Glu Arg Lys Gly Pro Thr Gly Ile 210 215 220 Gly His Ser Met Val Thr Asn Pro His Gly Glu Val Ile Ala Ser Ala 225 230 235 240 Gly Tyr Glu Pro Glu Met Leu Ile Ala Asp Ile Asp Val Ser Gly Leu 245 250 255 Ala Lys Ile Arg Glu Ala Leu Pro Val Leu 260 265 <210> 117 <211> 997 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(967) <223> RXA02560 <400> 117 ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc 60 ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115 Met Gln Gly Asn Ser 1 5 ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163 Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro 10 15 20 gga gaa ctt tta gcc gcc cgc tac gga caa cct gca acc tgg acg cca 211 Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro Ala Thr Trp Thr Pro 25 30 35 ccg cag tgg aat gag acg ctt gat gtc att cac cag cat cga tca gtt 259 Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His Gln His Arg Ser Val 40 45 50 cgc agg tgg ttg gat aaa ccg gtt gat gat gac acc atc cgc acc att 307 Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp Thr Ile Arg Thr Ile 55 60 65 att tcc gcc gca caa tcg gct gga acc tct tcc aat aag cag gtc att 355 Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser Asn Lys Gln Val Ile 70 75 80 85 tct gtc atc gtg gtt aaa gat cct gag ctg agg aaa ggc ctc gcg ggg 403 Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg Lys Gly Leu Ala Gly 90 95 100 atc act cgc cag atg ttt ccg cac ctt gag cag gtt ccc gcg gtg ctg 451 Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln Val Pro Ala Val Leu 105 110 115 att tgg ttg att gat tat tcc cga atc agt gcg gtg gca gcc aga gaa 499 Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala Val Ala Ala Arg Glu 120 125 130 gat ctc cca aca ggg gct ctt gat tat ctc gat gag gcc gcg ttg ggg 547 Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp Glu Ala Ala Leu Gly 135 140 145 ttc ctc gac gcc gga atc gca gct caa aac gct gca att gct gcg gag 595 Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala Ala Ile Ala Ala Glu 150 155 160 165 tca ctt gga ttg gga acg ctc tat ttg ggt tcg gtg cgc aac gat gcg 643 Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser Val Arg Asn Asp Ala 170 175 180 gaa gcc gtg cac aaa ttg ctt ggc ctt cca cct gag atc gtg cct gtc 691 Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro Glu Ile Val Pro Val 185 190 195 gtg ggc ttg gaa att ggg cat gcg gat ccg tct gaa cct gcc gga att 739 Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser Glu Pro Ala Gly Ile 200 205 210 aaa cct cgc ctg cca caa gaa gcc att gtt cac tgg gat acc tac acc 787 Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His Trp Asp Thr Tyr Thr 215 220 225 gag aaa aac ctc gaa ctt atc gat tcc tac gac cgc gcc ctc gac act 835 Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp Arg Ala Leu Asp Thr 230 235 240 245 tac tat tct cgc tac ggc cag cac cag ctc tgg tcg aag cag acg gcg 883 Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp Ser Lys Gln Thr Ala 250 255 260 cat agg gcg gcg tcg aaa agc ttt tca aaa acc aac agg cag ttc ctt 931 His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr Asn Arg Gln Phe Leu 265 270 275 agg ggc gtg ttt gag cgc gcc ggg ttt ggg ctg aga taaaagcatg 977 Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu Arg 280 285 attatggacg cctccctccc 997 <210> 118 <211> 289 <212> PRT <213> Corynebacterium glutamicum <400> 118 Met Gln Gly Asn Ser Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys 1 5 10 15 Pro Met Pro Ser Pro Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro 20 25 30 Ala Thr Trp Thr Pro Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His 35 40 45 Gln His Arg Ser Val Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp 50 55 60 Thr Ile Arg Thr Ile Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser 65 70 75 80 Asn Lys Gln Val Ile Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg 85 90 95 Lys Gly Leu Ala Gly Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln 100 105 110 Val Pro Ala Val Leu Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala 115 120 125 Val Ala Ala Arg Glu Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp 130 135 140 Glu Ala Ala Leu Gly Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala 145 150 155 160 Ala Ile Ala Ala Glu Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser 165 170 175 Val Arg Asn Asp Ala Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro 180 185 190 Glu Ile Val Pro Val Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser 195 200 205 Glu Pro Ala Gly Ile Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His 210 215 220 Trp Asp Thr Tyr Thr Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp 225 230 235 240 Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp 245 250 255 Ser Lys Gln Thr Ala His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr 260 265 270 Asn Arg Gln Phe Leu Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu 275 280 285 Arg <210> 119 <211> 2128 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(2098) <223> RXA02653 <400> 119 agacagagtg ttagtgcgtg gggcagctct cactttcatc gacatcactc gagtatgctc 60 accggccgta ttcattccaa taacccgcac agggaaacta atg ata ccg aag ccc 115 Met Ile Pro Lys Pro 1 5 gac gtg acc gac tta tat tta gag gac ctc tta aat gag ggt tcg gaa 163 Asp Val Thr Asp Leu Tyr Leu Glu Asp Leu Leu Asn Glu Gly Ser Glu 10 15 20 aag att cgg tcc gcc aag gat ctt tcc gaa ctt agg aca gtt cta aaa 211 Lys Ile Arg Ser Ala Lys Asp Leu Ser Glu Leu Arg Thr Val Leu Lys 25 30 35 gag gtt tcc tcc caa att cag gaa cga gct ggg aaa aaa gat gaa gaa 259 Glu Val Ser Ser Gln Ile Gln Glu Arg Ala Gly Lys Lys Asp Glu Glu 40 45 50 tgg gga atg ggg gcc act tgg cgg gag ctg tac ccc agc atc gtg gaa 307 Trp Gly Met Gly Ala Thr Trp Arg Glu Leu Tyr Pro Ser Ile Val Glu 55 60 65 cgc gct tcc tac gaa ggg cgt gac agc cta atc gga ttt gat cac tta 355 Arg Ala Ser Tyr Glu Gly Arg Asp Ser Leu Ile Gly Phe Asp His Leu 70 75 80 85 gcc cgg gaa atg gaa aga tta gcc ttc ggc cca cca tcc gaa agt ttt 403 Ala Arg Glu Met Glu Arg Leu Ala Phe Gly Pro Pro Ser Glu Ser Phe 90 95 100 gaa tac ctc caa gaa ctc gta aaa tcc gga gtg gta gac atc act cac 451 Glu Tyr Leu Gln Glu Leu Val Lys Ser Gly Val Val Asp Ile Thr His 105 110 115 ctg cat cgt ggc cgg gaa cca ctg aca gat tta gtt cgt gaa ctt gaa 499 Leu His Arg Gly Arg Glu Pro Leu Thr Asp Leu Val Arg Glu Leu Glu 120 125 130 ata act gtg gtg ata gac gct gtt ctt ccc ccg ccg gga gta gtg cca 547 Ile Thr Val Val Ile Asp Ala Val Leu Pro Pro Pro Gly Val Val Pro 135 140 145 ggc aca ttg gtg cac aat ttg gta aaa gag gga tat gcc aga atg cgt 595 Gly Thr Leu Val His Asn Leu Val Lys Glu Gly Tyr Ala Arg Met Arg 150 155 160 165 cct ggg act cgg ggg tta gat gta gcg gct gac ggc acc gtt caa ggg 643 Pro Gly Thr Arg Gly Leu Asp Val Ala Ala Asp Gly Thr Val Gln Gly 170 175 180 caa cga cat ttg gct gca gtc gga cgg atg acg gaa gat gtg gtt ttg 691 Gln Arg His Leu Ala Ala Val Gly Arg Met Thr Glu Asp Val Val Leu 185 190 195 ggt aat gac aca ttg tcg cga tca tta cat gac ata atc ccg aag tgg 739 Gly Asn Asp Thr Leu Ser Arg Ser Leu His Asp Ile Ile Pro Lys Trp 200 205 210 gct cgt cga gtt atc cgc gac gcg agc acg tat ccc gat agg gta cat 787 Ala Arg Arg Val Ile Arg Asp Ala Ser Thr Tyr Pro Asp Arg Val His 215 220 225 ggt act cca ccg ctt ccg gca cgg ttg gaa ccc tgg gcg gaa aag ctc 835 Gly Thr Pro Pro Leu Pro Ala Arg Leu Glu Pro Trp Ala Glu Lys Leu 230 235 240 245 act tca gat ccg gcc aca tgc cgc cac ctg att gaa gaa ttc ggg agt 883 Thr Ser Asp Pro Ala Thr Cys Arg His Leu Ile Glu Glu Phe Gly Ser 250 255 260 cct gtg aat gta ctc cat tca ggt tct atg cct cgt aat ata aat gag 931 Pro Val Asn Val Leu His Ser Gly Ser Met Pro Arg Asn Ile Asn Glu 265 270 275 ttg gtt gac gcc ggc att cag atg ggg gtg gat act cga ata ttt ttt 979 Leu Val Asp Ala Gly Ile Gln Met Gly Val Asp Thr Arg Ile Phe Phe 280 285 290 gcc cgc aaa gcg aat aag ggt ctt acc ttc gtt gat gcc gtt aaa gac 1027 Ala Arg Lys Ala Asn Lys Gly Leu Thr Phe Val Asp Ala Val Lys Asp 295 300 305 acc ggt cat ggt gta gat gta gcc agt gaa cga gag tta tct cag gtg 1075 Thr Gly His Gly Val Asp Val Ala Ser Glu Arg Glu Leu Ser Gln Val 310 315 320 325 ctt aat cgt gga gtc cca gga gag cgg atc att cta tcc gca gct atc 1123 Leu Asn Arg Gly Val Pro Gly Glu Arg Ile Ile Leu Ser Ala Ala Ile 330 335 340 aaa ccg gac aga cta ttg gca tta gcg atc gaa aat ggc gtg atc atc 1171 Lys Pro Asp Arg Leu Leu Ala Leu Ala Ile Glu Asn Gly Val Ile Ile 345 350 355 tct gtg gat tcg cgt gat gaa tta gat cgc att tcg gct ttg gtt ggt 1219 Ser Val Asp Ser Arg Asp Glu Leu Asp Arg Ile Ser Ala Leu Val Gly 360 365 370 gac cgc gtt gca cga gtt gcg cct aga gta gct cca gat cct gca gtc 1267 Asp Arg Val Ala Arg Val Ala Pro Arg Val Ala Pro Asp Pro Ala Val 375 380 385 tta cct cca act aga ttt ggt gag cgt gct gca gac tgg ggt aat cgg 1315 Leu Pro Pro Thr Arg Phe Gly Glu Arg Ala Ala Asp Trp Gly Asn Arg 390 395 400 405 ctt acc gag gtg ata ccc ggc gtg gat att gtg ggt ctt cac gtt cac 1363 Leu Thr Glu Val Ile Pro Gly Val Asp Ile Val Gly Leu His Val His 410 415 420 ctc cat ggc tat gct gca aaa gac cgt gct ctg gct ctg cag gaa tgt 1411 Leu His Gly Tyr Ala Ala Lys Asp Arg Ala Leu Ala Leu Gln Glu Cys 425 430 435 tgc caa ctc gtc gat tct ctc aga gaa tgc ggg cat tcc cca cag ttt 1459 Cys Gln Leu Val Asp Ser Leu Arg Glu Cys Gly His Ser Pro Gln Phe 440 445 450 att gac ctt gga gga ggg gtg cct atg agc tac att gaa tct gag gaa 1507 Ile Asp Leu Gly Gly Gly Val Pro Met Ser Tyr Ile Glu Ser Glu Glu 455 460 465 gat tgg atc cgt tat caa tcc gct aaa tct gcg act tca gcc ggg tat 1555 Asp Trp Ile Arg Tyr Gln Ser Ala Lys Ser Ala Thr Ser Ala Gly Tyr 470 475 480 485 gcc gaa tcc ttt acg tgg aaa gac gat ccg tta tct aat acg tac ccg 1603 Ala Glu Ser Phe Thr Trp Lys Asp Asp Pro Leu Ser Asn Thr Tyr Pro 490 495 500 ttc tat cag acc cca gtg cgc ggt aat tgg ttg aaa gac gtg ctt tct 1651 Phe Tyr Gln Thr Pro Val Arg Gly Asn Trp Leu Lys Asp Val Leu Ser 505 510 515 aag ggg gta gct cag atg ctc att gac cgg gga ttg cgg tta cac cta 1699 Lys Gly Val Ala Gln Met Leu Ile Asp Arg Gly Leu Arg Leu His Leu 520 525 530 gag cct ggt cga agt tta cta gat ggg tgt ggc gtc act ctt gcc gaa 1747 Glu Pro Gly Arg Ser Leu Leu Asp Gly Cys Gly Val Thr Leu Ala Glu 535 540 545 gtt gct ttt gtg aaa acc cga agt gac ggg ttg cct cta gtg gga ctg 1795 Val Ala Phe Val Lys Thr Arg Ser Asp Gly Leu Pro Leu Val Gly Leu 550 555 560 565 gct atg aac cga acg cag tgc cgg act aca tcc gat gat ttt ctc att 1843 Ala Met Asn Arg Thr Gln Cys Arg Thr Thr Ser Asp Asp Phe Leu Ile 570 575 580 gat ccc ctg cat atc act gac ggt gat gta ggc gag gaa atc gaa gca 1891 Asp Pro Leu His Ile Thr Asp Gly Asp Val Gly Glu Glu Ile Glu Ala 585 590 595 tat cta gtg ggt gcc tac tgc atc gaa gat gag ctg att tta cgc cgg 1939 Tyr Leu Val Gly Ala Tyr Cys Ile Glu Asp Glu Leu Ile Leu Arg Arg 600 605 610 cga atc cgc ttc ccg aga gga gtc aaa cca gga gat atc atc gga att 1987 Arg Ile Arg Phe Pro Arg Gly Val Lys Pro Gly Asp Ile Ile Gly Ile 615 620 625 cct aac acc gca gga tac ttc atg cat atc ttg gaa agt gca tcg cac 2035 Pro Asn Thr Ala Gly Tyr Phe Met His Ile Leu Glu Ser Ala Ser His 630 635 640 645 caa atc ccg ttg gcg aaa aat gta gtg tgg ccg gag ggg cag tta gac 2083 Gln Ile Pro Leu Ala Lys Asn Val Val Trp Pro Glu Gly Gln Leu Asp 650 655 660 gat atc gat gcg gat taagacataa ccattcgcta atctttcgac 2128 Asp Ile Asp Ala Asp 665 <210> 120 <211> 666 <212> PRT <213> Corynebacterium glutamicum <400> 120 Met Ile Pro Lys Pro Asp Val Thr Asp Leu Tyr Leu Glu Asp Leu Leu 1 5 10 15 Asn Glu Gly Ser Glu Lys Ile Arg Ser Ala Lys Asp Leu Ser Glu Leu 20 25 30 Arg Thr Val Leu Lys Glu Val Ser Ser Gln Ile Gln Glu Arg Ala Gly 35 40 45 Lys Lys Asp Glu Glu Trp Gly Met Gly Ala Thr Trp Arg Glu Leu Tyr 50 55 60 Pro Ser Ile Val Glu Arg Ala Ser Tyr Glu Gly Arg Asp Ser Leu Ile 65 70 75 80 Gly Phe Asp His Leu Ala Arg Glu Met Glu Arg Leu Ala Phe Gly Pro 85 90 95 Pro Ser Glu Ser Phe Glu Tyr Leu Gln Glu Leu Val Lys Ser Gly Val 100 105 110 Val Asp Ile Thr His Leu His Arg Gly Arg Glu Pro Leu Thr Asp Leu 115 120 125 Val Arg Glu Leu Glu Ile Thr Val Val Ile Asp Ala Val Leu Pro Pro 130 135 140 Pro Gly Val Val Pro Gly Thr Leu Val His Asn Leu Val Lys Glu Gly 145 150 155 160 Tyr Ala Arg Met Arg Pro Gly Thr Arg Gly Leu Asp Val Ala Ala Asp 165 170 175 Gly Thr Val Gln Gly Gln Arg His Leu Ala Ala Val Gly Arg Met Thr 180 185 190 Glu Asp Val Val Leu Gly Asn Asp Thr Leu Ser Arg Ser Leu His Asp 195 200 205 Ile Ile Pro Lys Trp Ala Arg Arg Val Ile Arg Asp Ala Ser Thr Tyr 210 215 220 Pro Asp Arg Val His Gly Thr Pro Pro Leu Pro Ala Arg Leu Glu Pro 225 230 235 240 Trp Ala Glu Lys Leu Thr Ser Asp Pro Ala Thr Cys Arg His Leu Ile 245 250 255 Glu Glu Phe Gly Ser Pro Val Asn Val Leu His Ser Gly Ser Met Pro 260 265 270 Arg Asn Ile Asn Glu Leu Val Asp Ala Gly Ile Gln Met Gly Val Asp 275 280 285 Thr Arg Ile Phe Phe Ala Arg Lys Ala Asn Lys Gly Leu Thr Phe Val 290 295 300 Asp Ala Val Lys Asp Thr Gly His Gly Val Asp Val Ala Ser Glu Arg 305 310 315 320 Glu Leu Ser Gln Val Leu Asn Arg Gly Val Pro Gly Glu Arg Ile Ile 325 330 335 Leu Ser Ala Ala Ile Lys Pro Asp Arg Leu Leu Ala Leu Ala Ile Glu 340 345 350 Asn Gly Val Ile Ile Ser Val Asp Ser Arg Asp Glu Leu Asp Arg Ile 355 360 365 Ser Ala Leu Val Gly Asp Arg Val Ala Arg Val Ala Pro Arg Val Ala 370 375 380 Pro Asp Pro Ala Val Leu Pro Pro Thr Arg Phe Gly Glu Arg Ala Ala 385 390 395 400 Asp Trp Gly Asn Arg Leu Thr Glu Val Ile Pro Gly Val Asp Ile Val 405 410 415 Gly Leu His Val His Leu His Gly Tyr Ala Ala Lys Asp Arg Ala Leu 420 425 430 Ala Leu Gln Glu Cys Cys Gln Leu Val Asp Ser Leu Arg Glu Cys Gly 435 440 445 His Ser Pro Gln Phe Ile Asp Leu Gly Gly Gly Val Pro Met Ser Tyr 450 455 460 Ile Glu Ser Glu Glu Asp Trp Ile Arg Tyr Gln Ser Ala Lys Ser Ala 465 470 475 480 Thr Ser Ala Gly Tyr Ala Glu Ser Phe Thr Trp Lys Asp Asp Pro Leu 485 490 495 Ser Asn Thr Tyr Pro Phe Tyr Gln Thr Pro Val Arg Gly Asn Trp Leu 500 505 510 Lys Asp Val Leu Ser Lys Gly Val Ala Gln Met Leu Ile Asp Arg Gly 515 520 525 Leu Arg Leu His Leu Glu Pro Gly Arg Ser Leu Leu Asp Gly Cys Gly 530 535 540 Val Thr Leu Ala Glu Val Ala Phe Val Lys Thr Arg Ser Asp Gly Leu 545 550 555 560 Pro Leu Val Gly Leu Ala Met Asn Arg Thr Gln Cys Arg Thr Thr Ser 565 570 575 Asp Asp Phe Leu Ile Asp Pro Leu His Ile Thr Asp Gly Asp Val Gly 580 585 590 Glu Glu Ile Glu Ala Tyr Leu Val Gly Ala Tyr Cys Ile Glu Asp Glu 595 600 605 Leu Ile Leu Arg Arg Arg Ile Arg Phe Pro Arg Gly Val Lys Pro Gly 610 615 620 Asp Ile Ile Gly Ile Pro Asn Thr Ala Gly Tyr Phe Met His Ile Leu 625 630 635 640 Glu Ser Ala Ser His Gln Ile Pro Leu Ala Lys Asn Val Val Trp Pro 645 650 655 Glu Gly Gln Leu Asp Asp Ile Asp Ala Asp 660 665 <210> 121 <211> 1468 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1438) <223> RXA02754 <400> 121 attatgaagc catcggagtt ggtgtggcct acaagggtga tcatgcgtgg atagtggtgg 60 agttcactgt agctcccgct gattccgtag aatcaacaga gtg aat acc aat ccg 115 Val Asn Thr Asn Pro 1 5 tct gaa ttc tcc tca aac cgt tca aca gct ctc ctt act gat aaa tat 163 Ser Glu Phe Ser Ser Asn Arg Ser Thr Ala Leu Leu Thr Asp Lys Tyr 10 15 20 gag ctg acc atg ctt caa gca gcg ctc gct gat ggt tct gca gaa cgc 211 Glu Leu Thr Met Leu Gln Ala Ala Leu Ala Asp Gly Ser Ala Glu Arg 25 30 35 ccc tca acg ttt gag gtc ttt agc cgc cgc ctc ccc aac gag cgc cga 259 Pro Ser Thr Phe Glu Val Phe Ser Arg Arg Leu Pro Asn Glu Arg Arg 40 45 50 tac ggt gtc gtc gca gga aca gca cga gtg ctg aag gcg att cgt gac 307 Tyr Gly Val Val Ala Gly Thr Ala Arg Val Leu Lys Ala Ile Arg Asp 55 60 65 ttt gta ttc aca gag gaa caa ctc gcc gat ctt gac ttt tta gac gac 355 Phe Val Phe Thr Glu Glu Gln Leu Ala Asp Leu Asp Phe Leu Asp Asp 70 75 80 85 cgt acc ctg gaa tac ctc cgc aac tac cga ttc acc ggc caa gtt gat 403 Arg Thr Leu Glu Tyr Leu Arg Asn Tyr Arg Phe Thr Gly Gln Val Asp 90 95 100 ggc tac cgc gaa ggc gaa atc tac ttc ccg cag tcc cct ctt ctg act 451 Gly Tyr Arg Glu Gly Glu Ile Tyr Phe Pro Gln Ser Pro Leu Leu Thr 105 110 115 gtg cgt ggc acg ttt gca gaa tgc gtc atc cta gaa act gtc att ttg 499 Val Arg Gly Thr Phe Ala Glu Cys Val Ile Leu Glu Thr Val Ile Leu 120 125 130 tcc atc atg aat gca gat tct gcc gtc gct tcc gcc gct gcg cgc atg 547 Ser Ile Met Asn Ala Asp Ser Ala Val Ala Ser Ala Ala Ala Arg Met 135 140 145 gtc acc gca gct gat ggt cgc ccc atc atc gaa atg gga tcc agg cgc 595 Val Thr Ala Ala Asp Gly Arg Pro Ile Ile Glu Met Gly Ser Arg Arg 150 155 160 165 acc cac gaa tac tcg gca gtc acc gca tcc cgc gca gca tac ctc gct 643 Thr His Glu Tyr Ser Ala Val Thr Ala Ser Arg Ala Ala Tyr Leu Ala 170 175 180 gga ttc tcc acc acc tcc aac ctc gag gcg gcc tac cgc tac gga att 691 Gly Phe Ser Thr Thr Ser Asn Leu Glu Ala Ala Tyr Arg Tyr Gly Ile 185 190 195 cca gca tcc gga acc tcc gcc cac gca tgg act ttg ctg cac atc aac 739 Pro Ala Ser Gly Thr Ser Ala His Ala Trp Thr Leu Leu His Ile Asn 200 205 210 gat gac ggc acc ccc aac gaa gca gca gct ttc aaa gca cag gtt gaa 787 Asp Asp Gly Thr Pro Asn Glu Ala Ala Ala Phe Lys Ala Gln Val Glu 215 220 225 tcc ctc ggc gtg gac acc acc ttg ctg gta gat act tat gac atc acc 835 Ser Leu Gly Val Asp Thr Thr Leu Leu Val Asp Thr Tyr Asp Ile Thr 230 235 240 245 caa ggt gtg gcc acc gcc att gaa gtt gca ggt cca gac ctt ggt ggc 883 Gln Gly Val Ala Thr Ala Ile Glu Val Ala Gly Pro Asp Leu Gly Gly 250 255 260 gta cgt atc gac tcc ggc gac cta ggt gtg ctt gcc cga aag gtc cgc 931 Val Arg Ile Asp Ser Gly Asp Leu Gly Val Leu Ala Arg Lys Val Arg 265 270 275 aag cag ctc gac gat ctc aac gcc cac aac acc aag att gtg gtc tcc 979 Lys Gln Leu Asp Asp Leu Asn Ala His Asn Thr Lys Ile Val Val Ser 280 285 290 tcc gac ctg gat gaa ttc gcc atc gcg ggt ctt cgc ggc gaa cca gtt 1027 Ser Asp Leu Asp Glu Phe Ala Ile Ala Gly Leu Arg Gly Glu Pro Val 295 300 305 gac gtc ttt ggc gtt ggc acc tcc gtt gtc aca ggt tct ggc gca cca 1075 Asp Val Phe Gly Val Gly Thr Ser Val Val Thr Gly Ser Gly Ala Pro 310 315 320 325 acc gct ggc ctc gtg tac aag atc gtg gaa gtt gcc ggt cac cct gtg 1123 Thr Ala Gly Leu Val Tyr Lys Ile Val Glu Val Ala Gly His Pro Val 330 335 340 gcc aag cgt tcc cga aac aag gaa agc tac ggt ggt ggc aag aag gct 1171 Ala Lys Arg Ser Arg Asn Lys Glu Ser Tyr Gly Gly Gly Lys Lys Ala 345 350 355 gtg cgc acc cac cgc aag tcc ggt acc gca atc gaa gaa atc gtc tac 1219 Val Arg Thr His Arg Lys Ser Gly Thr Ala Ile Glu Glu Ile Val Tyr 360 365 370 cca ttc aat gcc gaa gca cca gat act gga aag ctc gac act ttg agc 1267 Pro Phe Asn Ala Glu Ala Pro Asp Thr Gly Lys Leu Asp Thr Leu Ser 375 380 385 ctg acc atc cca ttg atg cgc gac ggt gaa atc gtt cca ggt ttg cct 1315 Leu Thr Ile Pro Leu Met Arg Asp Gly Glu Ile Val Pro Gly Leu Pro 390 395 400 405 act ttg gaa gat tcc cga gcg tat ttg gcc aag caa ttg gtc tct tta 1363 Thr Leu Glu Asp Ser Arg Ala Tyr Leu Ala Lys Gln Leu Val Ser Leu 410 415 420 cca tgg gaa ggc ctt gca ctg tct cgc gat gag cct gtt ttg cac act 1411 Pro Trp Glu Gly Leu Ala Leu Ser Arg Asp Glu Pro Val Leu His Thr 425 430 435 cgt ttc gtg ggt ttc ccg ccg gcc gct tagacaattc ggtctcacca 1458 Arg Phe Val Gly Phe Pro Pro Ala Ala 440 445 aaccacagtg 1468 <210> 122 <211> 446 <212> PRT <213> Corynebacterium glutamicum <400> 122 Val Asn Thr Asn Pro Ser Glu Phe Ser Ser Asn Arg Ser Thr Ala Leu 1 5 10 15 Leu Thr Asp Lys Tyr Glu Leu Thr Met Leu Gln Ala Ala Leu Ala Asp 20 25 30 Gly Ser Ala Glu Arg Pro Ser Thr Phe Glu Val Phe Ser Arg Arg Leu 35 40 45 Pro Asn Glu Arg Arg Tyr Gly Val Val Ala Gly Thr Ala Arg Val Leu 50 55 60 Lys Ala Ile Arg Asp Phe Val Phe Thr Glu Glu Gln Leu Ala Asp Leu 65 70 75 80 Asp Phe Leu Asp Asp Arg Thr Leu Glu Tyr Leu Arg Asn Tyr Arg Phe 85 90 95 Thr Gly Gln Val Asp Gly Tyr Arg Glu Gly Glu Ile Tyr Phe Pro Gln 100 105 110 Ser Pro Leu Leu Thr Val Arg Gly Thr Phe Ala Glu Cys Val Ile Leu 115 120 125 Glu Thr Val Ile Leu Ser Ile Met Asn Ala Asp Ser Ala Val Ala Ser 130 135 140 Ala Ala Ala Arg Met Val Thr Ala Ala Asp Gly Arg Pro Ile Ile Glu 145 150 155 160 Met Gly Ser Arg Arg Thr His Glu Tyr Ser Ala Val Thr Ala Ser Arg 165 170 175 Ala Ala Tyr Leu Ala Gly Phe Ser Thr Thr Ser Asn Leu Glu Ala Ala 180 185 190 Tyr Arg Tyr Gly Ile Pro Ala Ser Gly Thr Ser Ala His Ala Trp Thr 195 200 205 Leu Leu His Ile Asn Asp Asp Gly Thr Pro Asn Glu Ala Ala Ala Phe 210 215 220 Lys Ala Gln Val Glu Ser Leu Gly Val Asp Thr Thr Leu Leu Val Asp 225 230 235 240 Thr Tyr Asp Ile Thr Gln Gly Val Ala Thr Ala Ile Glu Val Ala Gly 245 250 255 Pro Asp Leu Gly Gly Val Arg Ile Asp Ser Gly Asp Leu Gly Val Leu 260 265 270 Ala Arg Lys Val Arg Lys Gln Leu Asp Asp Leu Asn Ala His Asn Thr 275 280 285 Lys Ile Val Val Ser Ser Asp Leu Asp Glu Phe Ala Ile Ala Gly Leu 290 295 300 Arg Gly Glu Pro Val Asp Val Phe Gly Val Gly Thr Ser Val Val Thr 305 310 315 320 Gly Ser Gly Ala Pro Thr Ala Gly Leu Val Tyr Lys Ile Val Glu Val 325 330 335 Ala Gly His Pro Val Ala Lys Arg Ser Arg Asn Lys Glu Ser Tyr Gly 340 345 350 Gly Gly Lys Lys Ala Val Arg Thr His Arg Lys Ser Gly Thr Ala Ile 355 360 365 Glu Glu Ile Val Tyr Pro Phe Asn Ala Glu Ala Pro Asp Thr Gly Lys 370 375 380 Leu Asp Thr Leu Ser Leu Thr Ile Pro Leu Met Arg Asp Gly Glu Ile 385 390 395 400 Val Pro Gly Leu Pro Thr Leu Glu Asp Ser Arg Ala Tyr Leu Ala Lys 405 410 415 Gln Leu Val Ser Leu Pro Trp Glu Gly Leu Ala Leu Ser Arg Asp Glu 420 425 430 Pro Val Leu His Thr Arg Phe Val Gly Phe Pro Pro Ala Ala 435 440 445 <210> 123 <211> 1468 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1438) <223> RXA02758 <400> 123 ggctggtgct tttcatattc cgacttgggg cacccctgaa tacatctcac ccaattcccc 60 ataactagac aattgcccag caacgactga taagtctcca atg tcg tgt tcc gcg 115 Met Ser Cys Ser Ala 1 5 ctc aga cat gag aca att gtt gcc gtg act gaa ctc atc cag aat gaa 163 Leu Arg His Glu Thr Ile Val Ala Val Thr Glu Leu Ile Gln Asn Glu 10 15 20 tcc caa gaa atc gct gag ctg gaa gcc ggc cag cag gtt gca ttg cgt 211 Ser Gln Glu Ile Ala Glu Leu Glu Ala Gly Gln Gln Val Ala Leu Arg 25 30 35 gaa ggt tat ctt cct gcg gtg atc aca gtg agc ggt aaa gac cgc cca 259 Glu Gly Tyr Leu Pro Ala Val Ile Thr Val Ser Gly Lys Asp Arg Pro 40 45 50 ggt gtg act gcc gcg ttc ttt agg gtc ttg tcc gct aat cag gtt cag 307 Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala Asn Gln Val Gln 55 60 65 gtc ttg gac gtt gag cag tca atg ttc cgt ggc ttt ttg aac ttg gcg 355 Val Leu Asp Val Glu Gln Ser Met Phe Arg Gly Phe Leu Asn Leu Ala 70 75 80 85 gcg ttt gtg ggt atc gca cct gag cgt gtc gag acc gtc acc aca ggc 403 Ala Phe Val Gly Ile Ala Pro Glu Arg Val Glu Thr Val Thr Thr Gly 90 95 100 ctg act gac acc ctc aag gtg cat gga cag tcc gtg gtg gtg gag ctg 451 Leu Thr Asp Thr Leu Lys Val His Gly Gln Ser Val Val Val Glu Leu 105 110 115 cag gaa act gtg cag tcg tcc cgt cct cgt tct tcc cat gtt gtt gtg 499 Gln Glu Thr Val Gln Ser Ser Arg Pro Arg Ser Ser His Val Val Val 120 125 130 gtg ttg ggt gat ccg gtt gat gcg ttg gat att tcc cgc att ggt cag 547 Val Leu Gly Asp Pro Val Asp Ala Leu Asp Ile Ser Arg Ile Gly Gln 135 140 145 acc ctg gcg gat tac gat gcc aac att gac acc att cgt ggt att tcg 595 Thr Leu Ala Asp Tyr Asp Ala Asn Ile Asp Thr Ile Arg Gly Ile Ser 150 155 160 165 gat tac cct gtg acc ggc ctg gag ctg aag gtg act gtg ccg gat gtc 643 Asp Tyr Pro Val Thr Gly Leu Glu Leu Lys Val Thr Val Pro Asp Val 170 175 180 agc cct ggt ggt ggt gaa gcg atg cgt aag gcg ctt gct gct ctt acc 691 Ser Pro Gly Gly Gly Glu Ala Met Arg Lys Ala Leu Ala Ala Leu Thr 185 190 195 tct gag ctg aat gtg gat att gcg att gag cgt tct ggt ttg ctg cgt 739 Ser Glu Leu Asn Val Asp Ile Ala Ile Glu Arg Ser Gly Leu Leu Arg 200 205 210 cgt tct aag cgt ctg gtg tgc ttc gat tgt gat tcc acg ttg atc act 787 Arg Ser Lys Arg Leu Val Cys Phe Asp Cys Asp Ser Thr Leu Ile Thr 215 220 225 ggt gag gtc att gag atg ctg gcg gct cac gcg ggc aag gaa gct gaa 835 Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly Lys Glu Ala Glu 230 235 240 245 gtt gcg gca gtt act gag cgt gcg atg cgc ggt gag ctc gat ttc gag 883 Val Ala Ala Val Thr Glu Arg Ala Met Arg Gly Glu Leu Asp Phe Glu 250 255 260 gag tct ctg cgt gag cgt gtg aag gcg ttg gct ggt ttg gat gcg tcg 931 Glu Ser Leu Arg Glu Arg Val Lys Ala Leu Ala Gly Leu Asp Ala Ser 265 270 275 gtg atc gat gag gtc gct gcc gct att gag ctg acc cct ggt gcg cgc 979 Val Ile Asp Glu Val Ala Ala Ala Ile Glu Leu Thr Pro Gly Ala Arg 280 285 290 acc acg atc cgt acg ctg aac cgc atg ggt tac cag acc gct gtt gtt 1027 Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr Gln Thr Ala Val Val 295 300 305 tcc ggt ggt ttc atc cag gtg ttg gaa ggt ttg gct gag gag ttg gag 1075 Ser Gly Gly Phe Ile Gln Val Leu Glu Gly Leu Ala Glu Glu Leu Glu 310 315 320 325 ttg gat tat gtc cgc gcc aac act ttg gaa atc gtt gat ggc aag ctg 1123 Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val Asp Gly Lys Leu 330 335 340 acc ggc aac gtc acc gga aag atc gtt gac cgc gct gcg aag gct gag 1171 Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg Ala Ala Lys Ala Glu 345 350 355 ttc ctc cgt gag ttc gct gcg gat tct ggc ctg aag atg tac cag act 1219 Phe Leu Arg Glu Phe Ala Ala Asp Ser Gly Leu Lys Met Tyr Gln Thr 360 365 370 gtc gct gtc ggt gat ggc gct aat gac atc gat atg ctc tcc gct gcg 1267 Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met Leu Ser Ala Ala 375 380 385 ggt ctg ggt gtt gct ttc aac gcg aag cct gcg ctg aag gag att gcg 1315 Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala Leu Lys Glu Ile Ala 390 395 400 405 gat act tcc gtg aac cac cca ttc ctc gac gag gtt ttg cac atc atg 1363 Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val Leu His Ile Met 410 415 420 ggc att tcc cgc gac gag atc gat ctg gcg gat cag gaa gac ggc act 1411 Gly Ile Ser Arg Asp Glu Ile Asp Leu Ala Asp Gln Glu Asp Gly Thr 425 430 435 ttc cac cgc gtt cca ttg acc aat gcc taaagattcg cttctcgacg 1458 Phe His Arg Val Pro Leu Thr Asn Ala 440 445 cccacctcct 1468 <210> 124 <211> 446 <212> PRT <213> Corynebacterium glutamicum <400> 124 Met Ser Cys Ser Ala Leu Arg His Glu Thr Ile Val Ala Val Thr Glu 1 5 10 15 Leu Ile Gln Asn Glu Ser Gln Glu Ile Ala Glu Leu Glu Ala Gly Gln 20 25 30 Gln Val Ala Leu Arg Glu Gly Tyr Leu Pro Ala Val Ile Thr Val Ser 35 40 45 Gly Lys Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser 50 55 60 Ala Asn Gln Val Gln Val Leu Asp Val Glu Gln Ser Met Phe Arg Gly 65 70 75 80 Phe Leu Asn Leu Ala Ala Phe Val Gly Ile Ala Pro Glu Arg Val Glu 85 90 95 Thr Val Thr Thr Gly Leu Thr Asp Thr Leu Lys Val His Gly Gln Ser 100 105 110 Val Val Val Glu Leu Gln Glu Thr Val Gln Ser Ser Arg Pro Arg Ser 115 120 125 Ser His Val Val Val Val Leu Gly Asp Pro Val Asp Ala Leu Asp Ile 130 135 140 Ser Arg Ile Gly Gln Thr Leu Ala Asp Tyr Asp Ala Asn Ile Asp Thr 145 150 155 160 Ile Arg Gly Ile Ser Asp Tyr Pro Val Thr Gly Leu Glu Leu Lys Val 165 170 175 Thr Val Pro Asp Val Ser Pro Gly Gly Gly Glu Ala Met Arg Lys Ala 180 185 190 Leu Ala Ala Leu Thr Ser Glu Leu Asn Val Asp Ile Ala Ile Glu Arg 195 200 205 Ser Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Cys Phe Asp Cys Asp 210 215 220 Ser Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala 225 230 235 240 Gly Lys Glu Ala Glu Val Ala Ala Val Thr Glu Arg Ala Met Arg Gly 245 250 255 Glu Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Lys Ala Leu Ala 260 265 270 Gly Leu Asp Ala Ser Val Ile Asp Glu Val Ala Ala Ala Ile Glu Leu 275 280 285 Thr Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr 290 295 300 Gln Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Gly Leu 305 310 315 320 Ala Glu Glu Leu Glu Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile 325 330 335 Val Asp Gly Lys Leu Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg 340 345 350 Ala Ala Lys Ala Glu Phe Leu Arg Glu Phe Ala Ala Asp Ser Gly Leu 355 360 365 Lys Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp 370 375 380 Met Leu Ser Ala Ala Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala 385 390 395 400 Leu Lys Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu 405 410 415 Val Leu His Ile Met Gly Ile Ser Arg Asp Glu Ile Asp Leu Ala Asp 420 425 430 Gln Glu Asp Gly Thr Phe His Arg Val Pro Leu Thr Asn Ala 435 440 445 <210> 125 <211> 1297 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1267) <223> RXA02790 <400> 125 cagtagaaat ccttcaaacc tggttggatg ctcgcacccg agcccttgaa ccacaatcca 60 cagacaccca agatttcgac gagaagggaa atttcccagg atg aac caa atc cga 115 Met Asn Gln Ile Arg 1 5 aac cgc cgg atg gag ccc gtc tac gta aag cgc cgc caa cgg ttt att 163 Asn Arg Arg Met Glu Pro Val Tyr Val Lys Arg Arg Gln Arg Phe Ile 10 15 20 gcc gtg acg atc gct tca ctc atc ctc att atc ggt gcc atc atc tat 211 Ala Val Thr Ile Ala Ser Leu Ile Leu Ile Ile Gly Ala Ile Ile Tyr 25 30 35 atc ggt gta gcc acc tca aac cgg acg cca cat gac tat gaa ggc tcc 259 Ile Gly Val Ala Thr Ser Asn Arg Thr Pro His Asp Tyr Glu Gly Ser 40 45 50 gga aac ggt gtg gtt cag ctg gtc gaa atc cct gaa ggt tcc tcc ata 307 Gly Asn Gly Val Val Gln Leu Val Glu Ile Pro Glu Gly Ser Ser Ile 55 60 65 tca gag ctc ggc cca gag ttg gaa gaa cga gat atc gtg gcc acc aac 355 Ser Glu Leu Gly Pro Glu Leu Glu Glu Arg Asp Ile Val Ala Thr Asn 70 75 80 85 tca gcg ttc caa aca gcg gcc agc aac aac ccc aac gcg ggt agt gta 403 Ser Ala Phe Gln Thr Ala Ala Ser Asn Asn Pro Asn Ala Gly Ser Val 90 95 100 cag cca ggt ttc tac cgt ctg cag gaa caa atg aac gca gca gct gca 451 Gln Pro Gly Phe Tyr Arg Leu Gln Glu Gln Met Asn Ala Ala Ala Ala 105 110 115 gtg tcg gct ctg ctt gat cca gac aac cag gtt gat ctc ctc gac att 499 Val Ser Ala Leu Leu Asp Pro Asp Asn Gln Val Asp Leu Leu Asp Ile 120 125 130 cac ggc ggc gcc acc ttg atg gac gtc act gtt gtc ggc gga aac acc 547 His Gly Gly Ala Thr Leu Met Asp Val Thr Val Val Gly Gly Asn Thr 135 140 145 cgc gcg gga atc tac tcc cag atc gca gcc gtg acc tgc acc gaa ggc 595 Arg Ala Gly Ile Tyr Ser Gln Ile Ala Ala Val Thr Cys Thr Glu Gly 150 155 160 165 tcc gcc aac tgc atc acc gct gag gat ttg cag cag gtt gcc tcc acc 643 Ser Ala Asn Cys Ile Thr Ala Glu Asp Leu Gln Gln Val Ala Ser Thr 170 175 180 gtg tcg cct gca gaa ttg ggt gtc cca gat tgg gca atc gct gct gtg 691 Val Ser Pro Ala Glu Leu Gly Val Pro Asp Trp Ala Ile Ala Ala Val 185 190 195 gaa gct cgc gga act gat cca aag cgc ctc gaa ggc ctg atc atg cct 739 Glu Ala Arg Gly Thr Asp Pro Lys Arg Leu Glu Gly Leu Ile Met Pro 200 205 210 ggc caa tac gtg gtg gat cca tcc aac gac gcc cag gga atc ctc acc 787 Gly Gln Tyr Val Val Asp Pro Ser Asn Asp Ala Gln Gly Ile Leu Thr 215 220 225 gat ctg atc acg cga tca gca aac cat ttc caa gaa acc gac atc acg 835 Asp Leu Ile Thr Arg Ser Ala Asn His Phe Gln Glu Thr Asp Ile Thr 230 235 240 245 ggc cgt gca gat gcc atc gga ctt act cca tat gag ctg gtc acc gca 883 Gly Arg Ala Asp Ala Ile Gly Leu Thr Pro Tyr Glu Leu Val Thr Ala 250 255 260 gca tct tta atc gag cgc gaa gca cca gca gga gat ttt gat aag gtc 931 Ala Ser Leu Ile Glu Arg Glu Ala Pro Ala Gly Asp Phe Asp Lys Val 265 270 275 gcc cgc gtc atc ttg aac cgt ctc gcc gag cca atg cag ctg caa ttc 979 Ala Arg Val Ile Leu Asn Arg Leu Ala Glu Pro Met Gln Leu Gln Phe 280 285 290 gac tcc acc gtc aac tac ggt ctg tct gaa caa gaa gta gca acc acc 1027 Asp Ser Thr Val Asn Tyr Gly Leu Ser Glu Gln Glu Val Ala Thr Thr 295 300 305 gac gaa gac cgt cag acc gtc acc cca tgg aac act tac gcc atg gac 1075 Asp Glu Asp Arg Gln Thr Val Thr Pro Trp Asn Thr Tyr Ala Met Asp 310 315 320 325 ggc ctg cca caa acc ccc atc gcc gca gta tcc acc gaa gca ctc caa 1123 Gly Leu Pro Gln Thr Pro Ile Ala Ala Val Ser Thr Glu Ala Leu Gln 330 335 340 gcc atg gaa aac cct gca gaa gga aac tgg ctg tac ttt gtc acc atc 1171 Ala Met Glu Asn Pro Ala Glu Gly Asn Trp Leu Tyr Phe Val Thr Ile 345 350 355 gac acc gat gga acc acc gtg ttc aac gac acc ttc gaa gag cac gaa 1219 Asp Thr Asp Gly Thr Thr Val Phe Asn Asp Thr Phe Glu Glu His Glu 360 365 370 gcc gac att gag caa gct ttg aac agt ggc gtt cta gac agc aac cga 1267 Ala Asp Ile Glu Gln Ala Leu Asn Ser Gly Val Leu Asp Ser Asn Arg 375 380 385 taaggatcag cgaataaaat tgggttctca 1297 <210> 126 <211> 389 <212> PRT <213> Corynebacterium glutamicum <400> 126 Met Asn Gln Ile Arg Asn Arg Arg Met Glu Pro Val Tyr Val Lys Arg 1 5 10 15 Arg Gln Arg Phe Ile Ala Val Thr Ile Ala Ser Leu Ile Leu Ile Ile 20 25 30 Gly Ala Ile Ile Tyr Ile Gly Val Ala Thr Ser Asn Arg Thr Pro His 35 40 45 Asp Tyr Glu Gly Ser Gly Asn Gly Val Val Gln Leu Val Glu Ile Pro 50 55 60 Glu Gly Ser Ser Ile Ser Glu Leu Gly Pro Glu Leu Glu Glu Arg Asp 65 70 75 80 Ile Val Ala Thr Asn Ser Ala Phe Gln Thr Ala Ala Ser Asn Asn Pro 85 90 95 Asn Ala Gly Ser Val Gln Pro Gly Phe Tyr Arg Leu Gln Glu Gln Met 100 105 110 Asn Ala Ala Ala Ala Val Ser Ala Leu Leu Asp Pro Asp Asn Gln Val 115 120 125 Asp Leu Leu Asp Ile His Gly Gly Ala Thr Leu Met Asp Val Thr Val 130 135 140 Val Gly Gly Asn Thr Arg Ala Gly Ile Tyr Ser Gln Ile Ala Ala Val 145 150 155 160 Thr Cys Thr Glu Gly Ser Ala Asn Cys Ile Thr Ala Glu Asp Leu Gln 165 170 175 Gln Val Ala Ser Thr Val Ser Pro Ala Glu Leu Gly Val Pro Asp Trp 180 185 190 Ala Ile Ala Ala Val Glu Ala Arg Gly Thr Asp Pro Lys Arg Leu Glu 195 200 205 Gly Leu Ile Met Pro Gly Gln Tyr Val Val Asp Pro Ser Asn Asp Ala 210 215 220 Gln Gly Ile Leu Thr Asp Leu Ile Thr Arg Ser Ala Asn His Phe Gln 225 230 235 240 Glu Thr Asp Ile Thr Gly Arg Ala Asp Ala Ile Gly Leu Thr Pro Tyr 245 250 255 Glu Leu Val Thr Ala Ala Ser Leu Ile Glu Arg Glu Ala Pro Ala Gly 260 265 270 Asp Phe Asp Lys Val Ala Arg Val Ile Leu Asn Arg Leu Ala Glu Pro 275 280 285 Met Gln Leu Gln Phe Asp Ser Thr Val Asn Tyr Gly Leu Ser Glu Gln 290 295 300 Glu Val Ala Thr Thr Asp Glu Asp Arg Gln Thr Val Thr Pro Trp Asn 305 310 315 320 Thr Tyr Ala Met Asp Gly Leu Pro Gln Thr Pro Ile Ala Ala Val Ser 325 330 335 Thr Glu Ala Leu Gln Ala Met Glu Asn Pro Ala Glu Gly Asn Trp Leu 340 345 350 Tyr Phe Val Thr Ile Asp Thr Asp Gly Thr Thr Val Phe Asn Asp Thr 355 360 365 Phe Glu Glu His Glu Ala Asp Ile Glu Gln Ala Leu Asn Ser Gly Val 370 375 380 Leu Asp Ser Asn Arg 385 <210> 127 <211> 883 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(853) <223> RXA02912 <400> 127 catcggtgtc ggttcactga tggtggccaa gaaggttgga taacagggtt aatcttgggt 60 gatattgaaa tgacgtattc ctgattgggc tgaaaaatct gtg aca tca cct gaa 115 Val Thr Ser Pro Glu 1 5 tta caa aac atc ctt aac aat tat tgg agc ggc agg gca gag gct tac 163 Leu Gln Asn Ile Leu Asn Asn Tyr Trp Ser Gly Arg Ala Glu Ala Tyr 10 15 20 cac ctc aac caa acc caa agc gag cgt gca caa ttt gaa cgc ccc atc 211 His Leu Asn Gln Thr Gln Ser Glu Arg Ala Gln Phe Glu Arg Pro Ile 25 30 35 tgg gaa aag gtg tgg tcg aag gct ttg cct atc gtg tcg gaa gaa gcg 259 Trp Glu Lys Val Trp Ser Lys Ala Leu Pro Ile Val Ser Glu Glu Ala 40 45 50 gta aag gtt ctc gat ctt ggc tgt ggc gct ggt tat gtc acc cac ctt 307 Val Lys Val Leu Asp Leu Gly Cys Gly Ala Gly Tyr Val Thr His Leu 55 60 65 cta agc gat tgc gga tac gaa aca atc ggc gtt gat ggt tct gag gaa 355 Leu Ser Asp Cys Gly Tyr Glu Thr Ile Gly Val Asp Gly Ser Glu Glu 70 75 80 85 atg atc aat caa gct acg cag gag aat ggt ctt cgc agg tcg acg ggt 403 Met Ile Asn Gln Ala Thr Gln Glu Asn Gly Leu Arg Arg Ser Thr Gly 90 95 100 cgg gcg act gcc att ttt cag gtc ggg gat gcg cat gat ccc gag ttc 451 Arg Ala Thr Ala Ile Phe Gln Val Gly Asp Ala His Asp Pro Glu Phe 105 110 115 cgg gaa ggc tct ttt gat gcg ata acc agc cgg tat gtg ttg tgg act 499 Arg Glu Gly Ser Phe Asp Ala Ile Thr Ser Arg Tyr Val Leu Trp Thr 120 125 130 ctg ctg gat ccc cag gca gcg att aat cgt tgg gtg tct ttg cta aaa 547 Leu Leu Asp Pro Gln Ala Ala Ile Asn Arg Trp Val Ser Leu Leu Lys 135 140 145 cct ggt ggg gtt att gcg tgc gta gat gcg gct tgg tat ccg aag ggc 595 Pro Gly Gly Val Ile Ala Cys Val Asp Ala Ala Trp Tyr Pro Lys Gly 150 155 160 165 att gat gct ggc acg gaa gta gat tca gtg gat ggt ccg agt gct ttc 643 Ile Asp Ala Gly Thr Glu Val Asp Ser Val Asp Gly Pro Ser Ala Phe 170 175 180 gta gag acc tat acc ccg gaa ctt ttg agg aat ctt ccc atg tca acg 691 Val Glu Thr Tyr Thr Pro Glu Leu Leu Arg Asn Leu Pro Met Ser Thr 185 190 195 acc tcc act ggc cac aat ttc gca gag ctt ttc cac aac gct ggc ctc 739 Thr Ser Thr Gly His Asn Phe Ala Glu Leu Phe His Asn Ala Gly Leu 200 205 210 aaa gaa gtc aca ttg aca ccc att gag ggg ctg gct gaa ctt gac cag 787 Lys Glu Val Thr Leu Thr Pro Ile Glu Gly Leu Ala Glu Leu Asp Gln 215 220 225 cga ttt ggc ctc tca cca ggg cat gag tcg act ccg cag ttc cta ttc 835 Arg Phe Gly Leu Ser Pro Gly His Glu Ser Thr Pro Gln Phe Leu Phe 230 235 240 245 agg gga att aaa tcc agt tagtgctgtt ttaagcggtc gagaatctgg 883 Arg Gly Ile Lys Ser Ser 250 <210> 128 <211> 251 <212> PRT <213> Corynebacterium glutamicum <400> 128 Val Thr Ser Pro Glu Leu Gln Asn Ile Leu Asn Asn Tyr Trp Ser Gly 1 5 10 15 Arg Ala Glu Ala Tyr His Leu Asn Gln Thr Gln Ser Glu Arg Ala Gln 20 25 30 Phe Glu Arg Pro Ile Trp Glu Lys Val Trp Ser Lys Ala Leu Pro Ile 35 40 45 Val Ser Glu Glu Ala Val Lys Val Leu Asp Leu Gly Cys Gly Ala Gly 50 55 60 Tyr Val Thr His Leu Leu Ser Asp Cys Gly Tyr Glu Thr Ile Gly Val 65 70 75 80 Asp Gly Ser Glu Glu Met Ile Asn Gln Ala Thr Gln Glu Asn Gly Leu 85 90 95 Arg Arg Ser Thr Gly Arg Ala Thr Ala Ile Phe Gln Val Gly Asp Ala 100 105 110 His Asp Pro Glu Phe Arg Glu Gly Ser Phe Asp Ala Ile Thr Ser Arg 115 120 125 Tyr Val Leu Trp Thr Leu Leu Asp Pro Gln Ala Ala Ile Asn Arg Trp 130 135 140 Val Ser Leu Leu Lys Pro Gly Gly Val Ile Ala Cys Val Asp Ala Ala 145 150 155 160 Trp Tyr Pro Lys Gly Ile Asp Ala Gly Thr Glu Val Asp Ser Val Asp 165 170 175 Gly Pro Ser Ala Phe Val Glu Thr Tyr Thr Pro Glu Leu Leu Arg Asn 180 185 190 Leu Pro Met Ser Thr Thr Ser Thr Gly His Asn Phe Ala Glu Leu Phe 195 200 205 His Asn Ala Gly Leu Lys Glu Val Thr Leu Thr Pro Ile Glu Gly Leu 210 215 220 Ala Glu Leu Asp Gln Arg Phe Gly Leu Ser Pro Gly His Glu Ser Thr 225 230 235 240 Pro Gln Phe Leu Phe Arg Gly Ile Lys Ser Ser 245 250 <210> 129 <211> 1498 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1468) <223> RXA02970 <400> 129 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag 60 ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag ggt 115 Leu Ala Leu Lys Gly 1 5 tac acc aac ttt gac ggt gaa ttc atc gaa ttc gga tct gtg caa gca 163 Tyr Thr Asn Phe Asp Gly Glu Phe Ile Glu Phe Gly Ser Val Gln Ala 10 15 20 aaa gaa gag gaa aaa cgg gca ttc gac aac gat cgc gcg cac gtt ttc 211 Lys Glu Glu Glu Lys Arg Ala Phe Asp Asn Asp Arg Ala His Val Phe 25 30 35 cac tcc tgg tcc gcg cag gac aaa atc agc ccc aaa gta tgg gca gct 259 His Ser Trp Ser Ala Gln Asp Lys Ile Ser Pro Lys Val Trp Ala Ala 40 45 50 gcc gaa ggt tcc acg ctg tac gac ttc gac ggc aac gcc ttc atc gac 307 Ala Glu Gly Ser Thr Leu Tyr Asp Phe Asp Gly Asn Ala Phe Ile Asp 55 60 65 atg ggt tcc caa ctt gtc tcg gca aac tta ggc cac aac aac cct cga 355 Met Gly Ser Gln Leu Val Ser Ala Asn Leu Gly His Asn Asn Pro Arg 70 75 80 85 tta gtt gag gcg atc cag cgc caa gca gcc cgg ttg acc aac atc aac 403 Leu Val Glu Ala Ile Gln Arg Gln Ala Ala Arg Leu Thr Asn Ile Asn 90 95 100 ccg gcc ttc ggc aat gat gtg cgc tct gat gtt gct gca aag atc gtg 451 Pro Ala Phe Gly Asn Asp Val Arg Ser Asp Val Ala Ala Lys Ile Val 105 110 115 tcg atg gcc cgt ggc gaa ttc tcc cac gtg ttt ttc acc aac ggc ggc 499 Ser Met Ala Arg Gly Glu Phe Ser His Val Phe Phe Thr Asn Gly Gly 120 125 130 gcc gac gcc atc gag cac tcc atc cgc atg gct cgc ctg cac acc gga 547 Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala Arg Leu His Thr Gly 135 140 145 cgc aac aaa att ctg tcc gca tac cgc agc tac cac ggc gca acc gga 595 Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr His Gly Ala Thr Gly 150 155 160 165 tcc gcg atg atg ctc acc ggc gaa cac cgc cgc ctg ggc aac ccc acc 643 Ser Ala Met Met Leu Thr Gly Glu His Arg Arg Leu Gly Asn Pro Thr 170 175 180 acc gac cca gat atc tac cac ttc tgg gca cca ttc ctg cac cac tcc 691 Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro Phe Leu His His Ser 185 190 195 tca ttc ttt gcc acc acc caa gaa gaa gaa tgc gaa cgc gca ctc aag 739 Ser Phe Phe Ala Thr Thr Gln Glu Glu Glu Cys Glu Arg Ala Leu Lys 200 205 210 cac ttg gaa gat gtc atc gcg ttt gaa ggt gct ggc atg atc gca gcg 787 His Leu Glu Asp Val Ile Ala Phe Glu Gly Ala Gly Met Ile Ala Ala 215 220 225 atc gtc ctg gag cca gtg gtg gga tca tca gga atc atc ctg cca cca 835 Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly Ile Ile Leu Pro Pro 230 235 240 245 gca ggt tac tta aat ggc gtg cgc gaa ctt tgc aac aag cac ggc atc 883 Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys Asn Lys His Gly Ile 250 255 260 ctc ttc atc gcc gac gaa gtc atg gtc gga ttc gga cgc acc gga aaa 931 Leu Phe Ile Ala Asp Glu Val Met Val Gly Phe Gly Arg Thr Gly Lys 265 270 275 ctg ttt gct tac gag cat gct ggc gac gat ttc cag cca gac atg atc 979 Leu Phe Ala Tyr Glu His Ala Gly Asp Asp Phe Gln Pro Asp Met Ile 280 285 290 acc ttc gcc aag ggt gtt aac gca ggt tac gcc cca ctc ggt ggc atc 1027 Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Ala Pro Leu Gly Gly Ile 295 300 305 gtg atg acc caa tca atc cgc gat acc ttc gga tca gag gca tac tcc 1075 Val Met Thr Gln Ser Ile Arg Asp Thr Phe Gly Ser Glu Ala Tyr Ser 310 315 320 325 ggc gga ctc acc tac tcc gga cac cca ctt gca gta gca ccc gcc aag 1123 Gly Gly Leu Thr Tyr Ser Gly His Pro Leu Ala Val Ala Pro Ala Lys 330 335 340 gca gcg ctg gag att tac gcg gaa gga gag atc att cca cgc gta gct 1171 Ala Ala Leu Glu Ile Tyr Ala Glu Gly Glu Ile Ile Pro Arg Val Ala 345 350 355 cga ctt ggc gct gaa ctg atc gaa cct cgc ctt cgt gaa cta gcg gaa 1219 Arg Leu Gly Ala Glu Leu Ile Glu Pro Arg Leu Arg Glu Leu Ala Glu 360 365 370 gaa aac gta gcg atc gct gac gtg cgg ggc atc gga ttc ttc tgg gca 1267 Glu Asn Val Ala Ile Ala Asp Val Arg Gly Ile Gly Phe Phe Trp Ala 375 380 385 gtg gag ttc aat gca gac gcc act gcc atg gct gcc ggt gct gca gaa 1315 Val Glu Phe Asn Ala Asp Ala Thr Ala Met Ala Ala Gly Ala Ala Glu 390 395 400 405 ttc aag gaa cgc ggc gtg tgg ccg atg atc tcc ggc aac cga ttc cac 1363 Phe Lys Glu Arg Gly Val Trp Pro Met Ile Ser Gly Asn Arg Phe His 410 415 420 atc gcg ccg ccg ctg acc acc act gat gac gaa ttg gta gca ctg ctg 1411 Ile Ala Pro Pro Leu Thr Thr Thr Asp Asp Glu Leu Val Ala Leu Leu 425 430 435 gac gcg gtg gaa gct gca gcc caa gct gtc gag ctg acc ttc gct ggg 1459 Asp Ala Val Glu Ala Ala Ala Gln Ala Val Glu Leu Thr Phe Ala Gly 440 445 450 gcg ttg ttc taagttttct agataacaag gccagcacag 1498 Ala Leu Phe 455 <210> 130 <211> 456 <212> PRT <213> Corynebacterium glutamicum <400> 130 Leu Ala Leu Lys Gly Tyr Thr Asn Phe Asp Gly Glu Phe Ile Glu Phe 1 5 10 15 Gly Ser Val Gln Ala Lys Glu Glu Glu Lys Arg Ala Phe Asp Asn Asp 20 25 30 Arg Ala His Val Phe His Ser Trp Ser Ala Gln Asp Lys Ile Ser Pro 35 40 45 Lys Val Trp Ala Ala Ala Glu Gly Ser Thr Leu Tyr Asp Phe Asp Gly 50 55 60 Asn Ala Phe Ile Asp Met Gly Ser Gln Leu Val Ser Ala Asn Leu Gly 65 70 75 80 His Asn Asn Pro Arg Leu Val Glu Ala Ile Gln Arg Gln Ala Ala Arg 85 90 95 Leu Thr Asn Ile Asn Pro Ala Phe Gly Asn Asp Val Arg Ser Asp Val 100 105 110 Ala Ala Lys Ile Val Ser Met Ala Arg Gly Glu Phe Ser His Val Phe 115 120 125 Phe Thr Asn Gly Gly Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala 130 135 140 Arg Leu His Thr Gly Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr 145 150 155 160 His Gly Ala Thr Gly Ser Ala Met Met Leu Thr Gly Glu His Arg Arg 165 170 175 Leu Gly Asn Pro Thr Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro 180 185 190 Phe Leu His His Ser Ser Phe Phe Ala Thr Thr Gln Glu Glu Glu Cys 195 200 205 Glu Arg Ala Leu Lys His Leu Glu Asp Val Ile Ala Phe Glu Gly Ala 210 215 220 Gly Met Ile Ala Ala Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly 225 230 235 240 Ile Ile Leu Pro Pro Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys 245 250 255 Asn Lys His Gly Ile Leu Phe Ile Ala Asp Glu Val Met Val Gly Phe 260 265 270 Gly Arg Thr Gly Lys Leu Phe Ala Tyr Glu His Ala Gly Asp Asp Phe 275 280 285 Gln Pro Asp Met Ile Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Ala 290 295 300 Pro Leu Gly Gly Ile Val Met Thr Gln Ser Ile Arg Asp Thr Phe Gly 305 310 315 320 Ser Glu Ala Tyr Ser Gly Gly Leu Thr Tyr Ser Gly His Pro Leu Ala 325 330 335 Val Ala Pro Ala Lys Ala Ala Leu Glu Ile Tyr Ala Glu Gly Glu Ile 340 345 350 Ile Pro Arg Val Ala Arg Leu Gly Ala Glu Leu Ile Glu Pro Arg Leu 355 360 365 Arg Glu Leu Ala Glu Glu Asn Val Ala Ile Ala Asp Val Arg Gly Ile 370 375 380 Gly Phe Phe Trp Ala Val Glu Phe Asn Ala Asp Ala Thr Ala Met Ala 385 390 395 400 Ala Gly Ala Ala Glu Phe Lys Glu Arg Gly Val Trp Pro Met Ile Ser 405 410 415 Gly Asn Arg Phe His Ile Ala Pro Pro Leu Thr Thr Thr Asp Asp Glu 420 425 430 Leu Val Ala Leu Leu Asp Ala Val Glu Ala Ala Ala Gln Ala Val Glu 435 440 445 Leu Thr Phe Ala Gly Ala Leu Phe 450 455 <210> 131 <211> 1231 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1201) <223> RXA03003 <400> 131 tcgatgatct gggctcccca ggtggtgcgg ctaagcttgg accacaagat tttgatcacc 60 caatgatcgc tgcgctgccg cctcaggcat aatctaacgc atg acc tct cgc acc 115 Met Thr Ser Arg Thr 1 5 ccg ctt gtt tct gtt ctt cct gat ttt ccg tgg gat tcg ctc gct tcc 163 Pro Leu Val Ser Val Leu Pro Asp Phe Pro Trp Asp Ser Leu Ala Ser 10 15 20 gca aaa gcc aaa gct gcg tct cac ccg gat ggg atc gtg aat ctt tct 211 Ala Lys Ala Lys Ala Ala Ser His Pro Asp Gly Ile Val Asn Leu Ser 25 30 35 gtt ggc act ccg gtt gat ccg gtc gcg ccc agc att cag atc gcg ttg 259 Val Gly Thr Pro Val Asp Pro Val Ala Pro Ser Ile Gln Ile Ala Leu 40 45 50 gca gaa gca gcg ggg ttt tcg ggt tac cct caa acc atc ggc acc ccg 307 Ala Glu Ala Ala Gly Phe Ser Gly Tyr Pro Gln Thr Ile Gly Thr Pro 55 60 65 gaa ctc cgc gca gcc atc agg ggc gcg ctt gag cgg cgc tac aac atg 355 Glu Leu Arg Ala Ala Ile Arg Gly Ala Leu Glu Arg Arg Tyr Asn Met 70 75 80 85 aca aag ctt gtc gac gcc tcc ctc ctc ccc gtc gtg ggt acc aag gag 403 Thr Lys Leu Val Asp Ala Ser Leu Leu Pro Val Val Gly Thr Lys Glu 90 95 100 gca att gcc ctt ctt cca ttc gcg ttg ggt att tcc ggc acc gtt gtc 451 Ala Ile Ala Leu Leu Pro Phe Ala Leu Gly Ile Ser Gly Thr Val Val 105 110 115 atc cca gag att gcg tac cca acc tac gaa gtc gct gtc gtg gcc gca 499 Ile Pro Glu Ile Ala Tyr Pro Thr Tyr Glu Val Ala Val Val Ala Ala 120 125 130 gga tgc acc gtg ttg cgt tct gat tcg ctg ttt aag ctc ggc ccg cag 547 Gly Cys Thr Val Leu Arg Ser Asp Ser Leu Phe Lys Leu Gly Pro Gln 135 140 145 atc ccg tcg atg atg ttt atc aac tca cca tcc aac ccc aca ggc aag 595 Ile Pro Ser Met Met Phe Ile Asn Ser Pro Ser Asn Pro Thr Gly Lys 150 155 160 165 gtt ctg ggc atc cca cac ttg cgc aag gtt gtg aag tgg gcg cag gaa 643 Val Leu Gly Ile Pro His Leu Arg Lys Val Val Lys Trp Ala Gln Glu 170 175 180 aac aac gtg atc ctc gca gct gat gaa tgc tac ttg ggt ctt ggc tgg 691 Asn Asn Val Ile Leu Ala Ala Asp Glu Cys Tyr Leu Gly Leu Gly Trp 185 190 195 gac gat gaa aac cca ccg atc tca att ttg gat cca cgt gtc tgc gat 739 Asp Asp Glu Asn Pro Pro Ile Ser Ile Leu Asp Pro Arg Val Cys Asp 200 205 210 ggc gac cac acc aac ttg atc gcc att cac tcg ctg tct aaa acc tca 787 Gly Asp His Thr Asn Leu Ile Ala Ile His Ser Leu Ser Lys Thr Ser 215 220 225 aac ctc gct tct tac cgc gca ggt tac ctc gtt ggc gat cca gcg ctg 835 Asn Leu Ala Ser Tyr Arg Ala Gly Tyr Leu Val Gly Asp Pro Ala Leu 230 235 240 245 att ggt gaa ctc acg gaa gtc cgt aag aac ttg ggt ctc atg gtt cct 883 Ile Gly Glu Leu Thr Glu Val Arg Lys Asn Leu Gly Leu Met Val Pro 250 255 260 ttc cca atc cag cag gcc atg atc gca gcc ctc aac gac gat gac caa 931 Phe Pro Ile Gln Gln Ala Met Ile Ala Ala Leu Asn Asp Asp Asp Gln 265 270 275 gag gca ggg cag aag ctc acc tac gcg att cgt cga gca aaa ctc atg 979 Glu Ala Gly Gln Lys Leu Thr Tyr Ala Ile Arg Arg Ala Lys Leu Met 280 285 290 cgc gcc ctg ttg gaa tcc ggc ttt cag gta gat aat tct gaa gcg ggt 1027 Arg Ala Leu Leu Glu Ser Gly Phe Gln Val Asp Asn Ser Glu Ala Gly 295 300 305 ctg tac ctc tgg gcg acg cgt gaa gaa cct tgc cgt gac act gtc gat 1075 Leu Tyr Leu Trp Ala Thr Arg Glu Glu Pro Cys Arg Asp Thr Val Asp 310 315 320 325 tgg ttc gct gag cgt ggc att ctc gtt gcc cca gga gac ttc tat ggc 1123 Trp Phe Ala Glu Arg Gly Ile Leu Val Ala Pro Gly Asp Phe Tyr Gly 330 335 340 cct cgc gga gcg cag cat gtg cgt gtg gcg atg acc gaa acc gac gag 1171 Pro Arg Gly Ala Gln His Val Arg Val Ala Met Thr Glu Thr Asp Glu 345 350 355 cgc gtc gac gcc ttt gtt tct cgc ctg agc taaacacgac taagctttat 1221 Arg Val Asp Ala Phe Val Ser Arg Leu Ser 360 365 ttgtttaatt 1231 <210> 132 <211> 367 <212> PRT <213> Corynebacterium glutamicum <400> 132 Met Thr Ser Arg Thr Pro Leu Val Ser Val Leu Pro Asp Phe Pro Trp 1 5 10 15 Asp Ser Leu Ala Ser Ala Lys Ala Lys Ala Ala Ser His Pro Asp Gly 20 25 30 Ile Val Asn Leu Ser Val Gly Thr Pro Val Asp Pro Val Ala Pro Ser 35 40 45 Ile Gln Ile Ala Leu Ala Glu Ala Ala Gly Phe Ser Gly Tyr Pro Gln 50 55 60 Thr Ile Gly Thr Pro Glu Leu Arg Ala Ala Ile Arg Gly Ala Leu Glu 65 70 75 80 Arg Arg Tyr Asn Met Thr Lys Leu Val Asp Ala Ser Leu Leu Pro Val 85 90 95 Val Gly Thr Lys Glu Ala Ile Ala Leu Leu Pro Phe Ala Leu Gly Ile 100 105 110 Ser Gly Thr Val Val Ile Pro Glu Ile Ala Tyr Pro Thr Tyr Glu Val 115 120 125 Ala Val Val Ala Ala Gly Cys Thr Val Leu Arg Ser Asp Ser Leu Phe 130 135 140 Lys Leu Gly Pro Gln Ile Pro Ser Met Met Phe Ile Asn Ser Pro Ser 145 150 155 160 Asn Pro Thr Gly Lys Val Leu Gly Ile Pro His Leu Arg Lys Val Val 165 170 175 Lys Trp Ala Gln Glu Asn Asn Val Ile Leu Ala Ala Asp Glu Cys Tyr 180 185 190 Leu Gly Leu Gly Trp Asp Asp Glu Asn Pro Pro Ile Ser Ile Leu Asp 195 200 205 Pro Arg Val Cys Asp Gly Asp His Thr Asn Leu Ile Ala Ile His Ser 210 215 220 Leu Ser Lys Thr Ser Asn Leu Ala Ser Tyr Arg Ala Gly Tyr Leu Val 225 230 235 240 Gly Asp Pro Ala Leu Ile Gly Glu Leu Thr Glu Val Arg Lys Asn Leu 245 250 255 Gly Leu Met Val Pro Phe Pro Ile Gln Gln Ala Met Ile Ala Ala Leu 260 265 270 Asn Asp Asp Asp Gln Glu Ala Gly Gln Lys Leu Thr Tyr Ala Ile Arg 275 280 285 Arg Ala Lys Leu Met Arg Ala Leu Leu Glu Ser Gly Phe Gln Val Asp 290 295 300 Asn Ser Glu Ala Gly Leu Tyr Leu Trp Ala Thr Arg Glu Glu Pro Cys 305 310 315 320 Arg Asp Thr Val Asp Trp Phe Ala Glu Arg Gly Ile Leu Val Ala Pro 325 330 335 Gly Asp Phe Tyr Gly Pro Arg Gly Ala Gln His Val Arg Val Ala Met 340 345 350 Thr Glu Thr Asp Glu Arg Val Asp Ala Phe Val Ser Arg Leu Ser 355 360 365 <210> 133 <211> 763 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(733) <223> RXA03171 <400> 133 atactttgtt ggactggaaa agtggccgtt tggttccctc caagcccaaa ttcgcccgcg 60 cggtcttctt ctgggcggca atgatttaac atgtgaagct atg gac atc acc atc 115 Met Asp Ile Thr Ile 1 5 gtc aac cac cca ctc gtt gct agc cgc cta acc ctg ttg cgc gac gag 163 Val Asn His Pro Leu Val Ala Ser Arg Leu Thr Leu Leu Arg Asp Glu 10 15 20 cgc agc gac aac gca gct ttc cgt gca gca gcc aac gac ctc ggc gcc 211 Arg Ser Asp Asn Ala Ala Phe Arg Ala Ala Ala Asn Asp Leu Gly Ala 25 30 35 atg ctg atc tac gaa gca tcc cga gat ctg gaa gtc gaa cac ttc gac 259 Met Leu Ile Tyr Glu Ala Ser Arg Asp Leu Glu Val Glu His Phe Asp 40 45 50 acc aaa acc ccc gtt gcc atg gct gaa ggt act cgc ctg aag cag cca 307 Thr Lys Thr Pro Val Ala Met Ala Glu Gly Thr Arg Leu Lys Gln Pro 55 60 65 ccc atc atc gtt ccc atc atc cgt gca ggt ctc ggc atg atc gac cca 355 Pro Ile Ile Val Pro Ile Ile Arg Ala Gly Leu Gly Met Ile Asp Pro 70 75 80 85 gcg ctg tcg atg att ccg gat gca cag gtc ggc ttc att ggc ctt gcc 403 Ala Leu Ser Met Ile Pro Asp Ala Gln Val Gly Phe Ile Gly Leu Ala 90 95 100 cgc gat gag gaa acc cat gag cca gtc cca tac ctt gag gcg ctg cca 451 Arg Asp Glu Glu Thr His Glu Pro Val Pro Tyr Leu Glu Ala Leu Pro 105 110 115 cag gat cta agc aac cag cct gta ttc ctt gtc gat ccc atg ctg gcc 499 Gln Asp Leu Ser Asn Gln Pro Val Phe Leu Val Asp Pro Met Leu Ala 120 125 130 acc ggc ggt tcc ctc ctg cac gcg atc cgc ctt ctt gct gat cgt ggc 547 Thr Gly Gly Ser Leu Leu His Ala Ile Arg Leu Leu Ala Asp Arg Gly 135 140 145 gcc acc gac atc acc gcc atc tgc atg gtt tct gcg cag cca ggt gtg 595 Ala Thr Asp Ile Thr Ala Ile Cys Met Val Ser Ala Gln Pro Gly Val 150 155 160 165 gac gca ttg gcg gaa tct ggt ctt cca gtt cgt ttg gtt acc gcc acc 643 Asp Ala Leu Ala Glu Ser Gly Leu Pro Val Arg Leu Val Thr Ala Thr 170 175 180 atc gac cca ggt cta gac gaa aac gcc tac atc gtg cct ggt ctt gga 691 Ile Asp Pro Gly Leu Asp Glu Asn Ala Tyr Ile Val Pro Gly Leu Gly 185 190 195 gat gcc ggt gat cgt ctc tac ggt ccg cga aac atc gac ctt 733 Asp Ala Gly Asp Arg Leu Tyr Gly Pro Arg Asn Ile Asp Leu 200 205 210 taaaagtttc taagggcatt acggaaaatt 763 <210> 134 <211> 211 <212> PRT <213> Corynebacterium glutamicum <400> 134 Met Asp Ile Thr Ile Val Asn His Pro Leu Val Ala Ser Arg Leu Thr 1 5 10 15 Leu Leu Arg Asp Glu Arg Ser Asp Asn Ala Ala Phe Arg Ala Ala Ala 20 25 30 Asn Asp Leu Gly Ala Met Leu Ile Tyr Glu Ala Ser Arg Asp Leu Glu 35 40 45 Val Glu His Phe Asp Thr Lys Thr Pro Val Ala Met Ala Glu Gly Thr 50 55 60 Arg Leu Lys Gln Pro Pro Ile Ile Val Pro Ile Ile Arg Ala Gly Leu 65 70 75 80 Gly Met Ile Asp Pro Ala Leu Ser Met Ile Pro Asp Ala Gln Val Gly 85 90 95 Phe Ile Gly Leu Ala Arg Asp Glu Glu Thr His Glu Pro Val Pro Tyr 100 105 110 Leu Glu Ala Leu Pro Gln Asp Leu Ser Asn Gln Pro Val Phe Leu Val 115 120 125 Asp Pro Met Leu Ala Thr Gly Gly Ser Leu Leu His Ala Ile Arg Leu 130 135 140 Leu Ala Asp Arg Gly Ala Thr Asp Ile Thr Ala Ile Cys Met Val Ser 145 150 155 160 Ala Gln Pro Gly Val Asp Ala Leu Ala Glu Ser Gly Leu Pro Val Arg 165 170 175 Leu Val Thr Ala Thr Ile Asp Pro Gly Leu Asp Glu Asn Ala Tyr Ile 180 185 190 Val Pro Gly Leu Gly Asp Ala Gly Asp Arg Leu Tyr Gly Pro Arg Asn 195 200 205 Ile Asp Leu 210 <210> 135 <211> 1288 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1258) <223> RXA03282 <400> 135 aggggctagt tttacacaaa agtggacagc ttggtctatc attgccagaa gaccggtcct 60 tttagggcca tagaattctg attacaggag ttgatctacc ttg tct ttt gac cca 115 Leu Ser Phe Asp Pro 1 5 aac acc cag ggt ttc tcc act gca tcg att cac gct ggg tat gag cca 163 Asn Thr Gln Gly Phe Ser Thr Ala Ser Ile His Ala Gly Tyr Glu Pro 10 15 20 gac gac tac tac ggt tcg att aac acc cca atc tat gcc tcc acc acc 211 Asp Asp Tyr Tyr Gly Ser Ile Asn Thr Pro Ile Tyr Ala Ser Thr Thr 25 30 35 ttc gcg cag aac gct cca aac gaa ctg cgc aaa ggc tac gag tac acc 259 Phe Ala Gln Asn Ala Pro Asn Glu Leu Arg Lys Gly Tyr Glu Tyr Thr 40 45 50 cgt gtg ggc aac ccc acc atc gtg gca tta gag cag acc gtc gca gca 307 Arg Val Gly Asn Pro Thr Ile Val Ala Leu Glu Gln Thr Val Ala Ala 55 60 65 ctc gaa ggc gca aag tat ggc cgc gca ttc tcc tcc ggc atg gct gca 355 Leu Glu Gly Ala Lys Tyr Gly Arg Ala Phe Ser Ser Gly Met Ala Ala 70 75 80 85 acc gac atc ctg ttc cgc atc atc ctc aag ccg ggc gat cac atc gtc 403 Thr Asp Ile Leu Phe Arg Ile Ile Leu Lys Pro Gly Asp His Ile Val 90 95 100 ctc ggc aac gat gct tac ggc gga acc tac cgc ctg atc gac acc gta 451 Leu Gly Asn Asp Ala Tyr Gly Gly Thr Tyr Arg Leu Ile Asp Thr Val 105 110 115 ttc acc gca tgg ggc gtc gaa tac acc gtt gtt gat acc tcc gtc gtg 499 Phe Thr Ala Trp Gly Val Glu Tyr Thr Val Val Asp Thr Ser Val Val 120 125 130 gaa gag gtc aag gca gcg atc aag gac aac acc aag ctg atc tgg gtg 547 Glu Glu Val Lys Ala Ala Ile Lys Asp Asn Thr Lys Leu Ile Trp Val 135 140 145 gaa acc cca acc aac cca gca ctt ggc atc acc gac atc gaa gca gta 595 Glu Thr Pro Thr Asn Pro Ala Leu Gly Ile Thr Asp Ile Glu Ala Val 150 155 160 165 gca aag ctc acc gaa ggc acc aac gcc aag ctg gtt gtt gac aac acc 643 Ala Lys Leu Thr Glu Gly Thr Asn Ala Lys Leu Val Val Asp Asn Thr 170 175 180 ttc gca tcc cca tac ctg cag cag cca cta aaa ctc ggc gca cac gca 691 Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Lys Leu Gly Ala His Ala 185 190 195 gtc ctg cac tcc acc acc aag tac atc gga gga cac tcc gac gtt gtt 739 Val Leu His Ser Thr Thr Lys Tyr Ile Gly Gly His Ser Asp Val Val 200 205 210 ggc ggc ctt gtg gtt acc aac gac cag gaa atg gac gaa gaa ctg ctg 787 Gly Gly Leu Val Val Thr Asn Asp Gln Glu Met Asp Glu Glu Leu Leu 215 220 225 ttc atg cag ggc ggc atc gga ccg atc cca tca gtt ttc gat gca tac 835 Phe Met Gln Gly Gly Ile Gly Pro Ile Pro Ser Val Phe Asp Ala Tyr 230 235 240 245 ctg acc gcc cgt ggc ctc aag acc ctt gca gtg cgc atg gat cgc cac 883 Leu Thr Ala Arg Gly Leu Lys Thr Leu Ala Val Arg Met Asp Arg His 250 255 260 tgc gac aac gca gaa aag atc gcg gaa ttc ctg gac tcc cgc cca gag 931 Cys Asp Asn Ala Glu Lys Ile Ala Glu Phe Leu Asp Ser Arg Pro Glu 265 270 275 gtc tcc acc gtg ctc tac cca ggt ctg aag aac cac cca ggc cac gaa 979 Val Ser Thr Val Leu Tyr Pro Gly Leu Lys Asn His Pro Gly His Glu 280 285 290 gtc gca gcg aag cag atg aag cgc ttc ggc ggc atg atc tcc gtc cgt 1027 Val Ala Ala Lys Gln Met Lys Arg Phe Gly Gly Met Ile Ser Val Arg 295 300 305 ttc gca ggc ggc gaa gaa gca gct aag aag ttc tgt acc tcc acc aaa 1075 Phe Ala Gly Gly Glu Glu Ala Ala Lys Lys Phe Cys Thr Ser Thr Lys 310 315 320 325 ctg atc tgt ctg gcc gag tcc ctc ggt ggc gtg gaa tcc ctc ctg gag 1123 Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val Glu Ser Leu Leu Glu 330 335 340 cac cca gca acc atg acc cac cag tca gct gcc ggc tct cag ctc gag 1171 His Pro Ala Thr Met Thr His Gln Ser Ala Ala Gly Ser Gln Leu Glu 345 350 355 gtt ccc cgc gac ctc gtg cgc atc tcc att ggt att gaa gac att gaa 1219 Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly Ile Glu Asp Ile Glu 360 365 370 gac ctg ctc gca gat gtc gag cag gcc ctc aat aac ctt tagaaactat 1268 Asp Leu Leu Ala Asp Val Glu Gln Ala Leu Asn Asn Leu 375 380 385 ttggcggcaa gcagcttttc 1288 <210> 136 <211> 386 <212> PRT <213> Corynebacterium glutamicum <400> 136 Leu Ser Phe Asp Pro Asn Thr Gln Gly Phe Ser Thr Ala Ser Ile His 1 5 10 15 Ala Gly Tyr Glu Pro Asp Asp Tyr Tyr Gly Ser Ile Asn Thr Pro Ile 20 25 30 Tyr Ala Ser Thr Thr Phe Ala Gln Asn Ala Pro Asn Glu Leu Arg Lys 35 40 45 Gly Tyr Glu Tyr Thr Arg Val Gly Asn Pro Thr Ile Val Ala Leu Glu 50 55 60 Gln Thr Val Ala Ala Leu Glu Gly Ala Lys Tyr Gly Arg Ala Phe Ser 65 70 75 80 Ser Gly Met Ala Ala Thr Asp Ile Leu Phe Arg Ile Ile Leu Lys Pro 85 90 95 Gly Asp His Ile Val Leu Gly Asn Asp Ala Tyr Gly Gly Thr Tyr Arg 100 105 110 Leu Ile Asp Thr Val Phe Thr Ala Trp Gly Val Glu Tyr Thr Val Val 115 120 125 Asp Thr Ser Val Val Glu Glu Val Lys Ala Ala Ile Lys Asp Asn Thr 130 135 140 Lys Leu Ile Trp Val Glu Thr Pro Thr Asn Pro Ala Leu Gly Ile Thr 145 150 155 160 Asp Ile Glu Ala Val Ala Lys Leu Thr Glu Gly Thr Asn Ala Lys Leu 165 170 175 Val Val Asp Asn Thr Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Lys 180 185 190 Leu Gly Ala His Ala Val Leu His Ser Thr Thr Lys Tyr Ile Gly Gly 195 200 205 His Ser Asp Val Val Gly Gly Leu Val Val Thr Asn Asp Gln Glu Met 210 215 220 Asp Glu Glu Leu Leu Phe Met Gln Gly Gly Ile Gly Pro Ile Pro Ser 225 230 235 240 Val Phe Asp Ala Tyr Leu Thr Ala Arg Gly Leu Lys Thr Leu Ala Val 245 250 255 Arg Met Asp Arg His Cys Asp Asn Ala Glu Lys Ile Ala Glu Phe Leu 260 265 270 Asp Ser Arg Pro Glu Val Ser Thr Val Leu Tyr Pro Gly Leu Lys Asn 275 280 285 His Pro Gly His Glu Val Ala Ala Lys Gln Met Lys Arg Phe Gly Gly 290 295 300 Met Ile Ser Val Arg Phe Ala Gly Gly Glu Glu Ala Ala Lys Lys Phe 305 310 315 320 Cys Thr Ser Thr Lys Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val 325 330 335 Glu Ser Leu Leu Glu His Pro Ala Thr Met Thr His Gln Ser Ala Ala 340 345 350 Gly Ser Gln Leu Glu Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly 355 360 365 Ile Glu Asp Ile Glu Asp Leu Leu Ala Asp Val Glu Gln Ala Leu Asn 370 375 380 Asn Leu 385 <210> 137 <211> 1336 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1306) <223> RXA03465 <400> 137 gatgaggtgg ataaaacctc cgtcggtaaa tatgacaaga aggacctccg caaccacctg 60 cgcaacggcg atttcgaagt aattaagctt aaagggccag gtg aaa aat aac ttc 115 Val Lys Asn Asn Phe 1 5 cct att tat tcc ggc ttt tac tgg ctt tat tcc gca gct ctt tct agg 163 Pro Ile Tyr Ser Gly Phe Tyr Trp Leu Tyr Ser Ala Ala Leu Ser Arg 10 15 20 atg ggc aag aag atg act act cgc ctt tat ctt tcg cca acg ccg ccc 211 Met Gly Lys Lys Met Thr Thr Arg Leu Tyr Leu Ser Pro Thr Pro Pro 25 30 35 cga aat gat cgg gag ggg gcg tcg aaa agc act tct gcg agc atc aaa 259 Arg Asn Asp Arg Glu Gly Ala Ser Lys Ser Thr Ser Ala Ser Ile Lys 40 45 50 cat gat gcg tac ctg cca ccc gca gac ggc aat cgc gtg ctt gtg gac 307 His Asp Ala Tyr Leu Pro Pro Ala Asp Gly Asn Arg Val Leu Val Asp 55 60 65 aga ttc gga cgc atc gcg cgt gac ctg cgg gtg tca ctg acc gac cga 355 Arg Phe Gly Arg Ile Ala Arg Asp Leu Arg Val Ser Leu Thr Asp Arg 70 75 80 85 tgc aac ctc cgc tgc acc tat tgc atg ccc gcg gag ggt tta gag tgg 403 Cys Asn Leu Arg Cys Thr Tyr Cys Met Pro Ala Glu Gly Leu Glu Trp 90 95 100 ctg ccc acc gag cag acg ctt aac gac gcc gag gtg ctg cga ctc atc 451 Leu Pro Thr Glu Gln Thr Leu Asn Asp Ala Glu Val Leu Arg Leu Ile 105 110 115 cgc att gcg gtg gtt aag ctg ggc att cgt caa att cga ttc acc ggc 499 Arg Ile Ala Val Val Lys Leu Gly Ile Arg Gln Ile Arg Phe Thr Gly 120 125 130 ggc gag cct tta ctg cgg aaa aat ttg gaa gac atc atc gcc ggc acc 547 Gly Glu Pro Leu Leu Arg Lys Asn Leu Glu Asp Ile Ile Ala Gly Thr 135 140 145 gca gcc ctg cgc acc gac gaa ggc gaa aaa gtt cac atc gct ctc acc 595 Ala Ala Leu Arg Thr Asp Glu Gly Glu Lys Val His Ile Ala Leu Thr 150 155 160 165 acc aac ggc ctt ggc cta gac aaa cgc atc gca gga ctg aaa gaa gct 643 Thr Asn Gly Leu Gly Leu Asp Lys Arg Ile Ala Gly Leu Lys Glu Ala 170 175 180 ggt ctt gac cgg gtc aat att tca ctc gac acc atc gac gcc gaa cgc 691 Gly Leu Asp Arg Val Asn Ile Ser Leu Asp Thr Ile Asp Ala Glu Arg 185 190 195 tac gtc tcg cta acc agg cgt gat cga ttg tcc ggt gtg ttg gcg tcc 739 Tyr Val Ser Leu Thr Arg Arg Asp Arg Leu Ser Gly Val Leu Ala Ser 200 205 210 atc gat gcc gct gtt gcc gct ggc ctt cac cca gtg aag atc aac gcc 787 Ile Asp Ala Ala Val Ala Ala Gly Leu His Pro Val Lys Ile Asn Ala 215 220 225 gtg gtc atg cct ggg gtc aat gaa gta gat atc gtc ccc ctt gcg gaa 835 Val Val Met Pro Gly Val Asn Glu Val Asp Ile Val Pro Leu Ala Glu 230 235 240 245 tac tgc att tcc aaa ggc tcc caa ctg cga ttc atc gaa caa atg cca 883 Tyr Cys Ile Ser Lys Gly Ser Gln Leu Arg Phe Ile Glu Gln Met Pro 250 255 260 ctt ggc ccg cgc gag cag tgg aaa cgc ggc gat atg gtc aca gcc gaa 931 Leu Gly Pro Arg Glu Gln Trp Lys Arg Gly Asp Met Val Thr Ala Glu 265 270 275 gaa atc ctg gcg cgc ctg gaa gaa aaa ttc acc tta tcc ccc gcc aag 979 Glu Ile Leu Ala Arg Leu Glu Glu Lys Phe Thr Leu Ser Pro Ala Lys 280 285 290 gaa ccc cga gga gct gca cct gct gcg ctg tgg aat gtg gta gat aaa 1027 Glu Pro Arg Gly Ala Ala Pro Ala Ala Leu Trp Asn Val Val Asp Lys 295 300 305 tcc aac cct gat atc act gga caa atc ggc atc atc gcc tcg gtg acg 1075 Ser Asn Pro Asp Ile Thr Gly Gln Ile Gly Ile Ile Ala Ser Val Thr 310 315 320 325 cac cca ttt tgc gga gat tgc gat cgc tcc cgc ctc acc acc gac ggc 1123 His Pro Phe Cys Gly Asp Cys Asp Arg Ser Arg Leu Thr Thr Asp Gly 330 335 340 acc atc cga aac tgc ctt ttc tcc cgc act gaa act ccc cta cgt gac 1171 Thr Ile Arg Asn Cys Leu Phe Ser Arg Thr Glu Thr Pro Leu Arg Asp 345 350 355 gcg ctt cgc gac ggc gcc tcc gac gat gag ctc gcg caa ctg tgg gca 1219 Ala Leu Arg Asp Gly Ala Ser Asp Asp Glu Leu Ala Gln Leu Trp Ala 360 365 370 ggc gcc atg tgg gag aag aaa ccc ggc cat ggc atc gac gat gaa ggc 1267 Gly Ala Met Trp Glu Lys Lys Pro Gly His Gly Ile Asp Asp Glu Gly 375 380 385 ttc ctc caa cca gat cgc ccc atg tct gcc atc ggt ggc tagcccatac 1316 Phe Leu Gln Pro Asp Arg Pro Met Ser Ala Ile Gly Gly 390 395 400 cagccccaac cagacaggcg 1336 <210> 138 <211> 402 <212> PRT <213> Corynebacterium glutamicum <400> 138 Val Lys Asn Asn Phe Pro Ile Tyr Ser Gly Phe Tyr Trp Leu Tyr Ser 1 5 10 15 Ala Ala Leu Ser Arg Met Gly Lys Lys Met Thr Thr Arg Leu Tyr Leu 20 25 30 Ser Pro Thr Pro Pro Arg Asn Asp Arg Glu Gly Ala Ser Lys Ser Thr 35 40 45 Ser Ala Ser Ile Lys His Asp Ala Tyr Leu Pro Pro Ala Asp Gly Asn 50 55 60 Arg Val Leu Val Asp Arg Phe Gly Arg Ile Ala Arg Asp Leu Arg Val 65 70 75 80 Ser Leu Thr Asp Arg Cys Asn Leu Arg Cys Thr Tyr Cys Met Pro Ala 85 90 95 Glu Gly Leu Glu Trp Leu Pro Thr Glu Gln Thr Leu Asn Asp Ala Glu 100 105 110 Val Leu Arg Leu Ile Arg Ile Ala Val Val Lys Leu Gly Ile Arg Gln 115 120 125 Ile Arg Phe Thr Gly Gly Glu Pro Leu Leu Arg Lys Asn Leu Glu Asp 130 135 140 Ile Ile Ala Gly Thr Ala Ala Leu Arg Thr Asp Glu Gly Glu Lys Val 145 150 155 160 His Ile Ala Leu Thr Thr Asn Gly Leu Gly Leu Asp Lys Arg Ile Ala 165 170 175 Gly Leu Lys Glu Ala Gly Leu Asp Arg Val Asn Ile Ser Leu Asp Thr 180 185 190 Ile Asp Ala Glu Arg Tyr Val Ser Leu Thr Arg Arg Asp Arg Leu Ser 195 200 205 Gly Val Leu Ala Ser Ile Asp Ala Ala Val Ala Ala Gly Leu His Pro 210 215 220 Val Lys Ile Asn Ala Val Val Met Pro Gly Val Asn Glu Val Asp Ile 225 230 235 240 Val Pro Leu Ala Glu Tyr Cys Ile Ser Lys Gly Ser Gln Leu Arg Phe 245 250 255 Ile Glu Gln Met Pro Leu Gly Pro Arg Glu Gln Trp Lys Arg Gly Asp 260 265 270 Met Val Thr Ala Glu Glu Ile Leu Ala Arg Leu Glu Glu Lys Phe Thr 275 280 285 Leu Ser Pro Ala Lys Glu Pro Arg Gly Ala Ala Pro Ala Ala Leu Trp 290 295 300 Asn Val Val Asp Lys Ser Asn Pro Asp Ile Thr Gly Gln Ile Gly Ile 305 310 315 320 Ile Ala Ser Val Thr His Pro Phe Cys Gly Asp Cys Asp Arg Ser Arg 325 330 335 Leu Thr Thr Asp Gly Thr Ile Arg Asn Cys Leu Phe Ser Arg Thr Glu 340 345 350 Thr Pro Leu Arg Asp Ala Leu Arg Asp Gly Ala Ser Asp Asp Glu Leu 355 360 365 Ala Gln Leu Trp Ala Gly Ala Met Trp Glu Lys Lys Pro Gly His Gly 370 375 380 Ile Asp Asp Glu Gly Phe Leu Gln Pro Asp Arg Pro Met Ser Ala Ile 385 390 395 400 Gly Gly <210> 139 <211> 1402 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1372) <223> RXA03677 <400> 139 atttttgacc ctccgggggt gatttaacct aaaattccac acaaacgtgt tcgaggtcat 60 tagattgata agcatctgtt gttaagaaag gtgacttcct atg tcc tcg att tcc 115 Met Ser Ser Ile Ser 1 5 cgc aag acc ggc gcg tca ctt gca gcc acc aca ctg ttg gca gcg atc 163 Arg Lys Thr Gly Ala Ser Leu Ala Ala Thr Thr Leu Leu Ala Ala Ile 10 15 20 gca ctg gcc ggt tgt agt tca gac tca agc tcc gac tcc aca gat tcc 211 Ala Leu Ala Gly Cys Ser Ser Asp Ser Ser Ser Asp Ser Thr Asp Ser 25 30 35 acc gct agc gaa ggc gca gac agc cgc ggc ccc atc acc ttt gcg atg 259 Thr Ala Ser Glu Gly Ala Asp Ser Arg Gly Pro Ile Thr Phe Ala Met 40 45 50 ggc aaa aac gac acc gac aaa gtc att ccg atc atc gac cgc tgg aac 307 Gly Lys Asn Asp Thr Asp Lys Val Ile Pro Ile Ile Asp Arg Trp Asn 55 60 65 gaa gcc cac ccc gat gag cag gta acg ctc aac gaa ctc gcc ggt gaa 355 Glu Ala His Pro Asp Glu Gln Val Thr Leu Asn Glu Leu Ala Gly Glu 70 75 80 85 gcc gac gcg cag cgc gaa acc ctc gtg caa tcc ctg cag gcc ggc aac 403 Ala Asp Ala Gln Arg Glu Thr Leu Val Gln Ser Leu Gln Ala Gly Asn 90 95 100 tct gac tac gac gtc atg gcg ctc gac gtc atc tgg acc gca gac ttc 451 Ser Asp Tyr Asp Val Met Ala Leu Asp Val Ile Trp Thr Ala Asp Phe 105 110 115 gcg gca aac caa tgg ctc gca cca ctt gaa ggc gac ctc gag gta gac 499 Ala Ala Asn Gln Trp Leu Ala Pro Leu Glu Gly Asp Leu Glu Val Asp 120 125 130 acc tcc gga ctg ctg caa tcc acc gtg gat tcc gca acc tac aac ggc 547 Thr Ser Gly Leu Leu Gln Ser Thr Val Asp Ser Ala Thr Tyr Asn Gly 135 140 145 acc ctc tac gca ctg cca cag aac acc aac ggc cag cta ctg ttc cgc 595 Thr Leu Tyr Ala Leu Pro Gln Asn Thr Asn Gly Gln Leu Leu Phe Arg 150 155 160 165 aac acc gaa atc atc cca gaa gca cca gca aac tgg gct gac ctc gtg 643 Asn Thr Glu Ile Ile Pro Glu Ala Pro Ala Asn Trp Ala Asp Leu Val 170 175 180 gaa tcc tgc acg ctt gct gaa gaa gca ggc gtt gat tgc ctg acc act 691 Glu Ser Cys Thr Leu Ala Glu Glu Ala Gly Val Asp Cys Leu Thr Thr 185 190 195 cag ctc aag cag tac gaa ggc ctt tca gtg aac acc atc ggc ttc atc 739 Gln Leu Lys Gln Tyr Glu Gly Leu Ser Val Asn Thr Ile Gly Phe Ile 200 205 210 gaa ggt tgg gga ggc agc gtc cta gac gat gac ggc aac gtc acc gta 787 Glu Gly Trp Gly Gly Ser Val Leu Asp Asp Asp Gly Asn Val Thr Val 215 220 225 gac agc gac gac gcc aag gca ggc ctt caa gcg ctt gtc gac ggc ttc 835 Asp Ser Asp Asp Ala Lys Ala Gly Leu Gln Ala Leu Val Asp Gly Phe 230 235 240 245 gac gac ggc acc atc tcc aag gca tcc ctt gca gcg acc gaa gaa gaa 883 Asp Asp Gly Thr Ile Ser Lys Ala Ser Leu Ala Ala Thr Glu Glu Glu 250 255 260 acc aac ctc gca ttc acc gaa ggc caa acc gcc tac gcc att aac tgg 931 Thr Asn Leu Ala Phe Thr Glu Gly Gln Thr Ala Tyr Ala Ile Asn Trp 265 270 275 cca tac atg tac acc aac tcc gaa gaa gcc gaa gca acc gca ggc aaa 979 Pro Tyr Met Tyr Thr Asn Ser Glu Glu Ala Glu Ala Thr Ala Gly Lys 280 285 290 ttc gaa gta cag ccc ctc gta ggt aaa gac ggc gtc ggc gta tcc acc 1027 Phe Glu Val Gln Pro Leu Val Gly Lys Asp Gly Val Gly Val Ser Thr 295 300 305 ctt ggt ggc tac aac aac ggc atc aac gtc aac tcc gaa aac aag gca 1075 Leu Gly Gly Tyr Asn Asn Gly Ile Asn Val Asn Ser Glu Asn Lys Ala 310 315 320 325 acc gcc cgc gac ttc atc gaa ttc atc atc aac gaa gag aac caa acc 1123 Thr Ala Arg Asp Phe Ile Glu Phe Ile Ile Asn Glu Glu Asn Gln Thr 330 335 340 tgg ttc gcg gac aac tcc ttc cca cca gtt ctg gca tcc atc tac gat 1171 Trp Phe Ala Asp Asn Ser Phe Pro Pro Val Leu Ala Ser Ile Tyr Asp 345 350 355 gat gag tcc ctt gtt gag cag tac cca tac ctg cca gca ctg aag gaa 1219 Asp Glu Ser Leu Val Glu Gln Tyr Pro Tyr Leu Pro Ala Leu Lys Glu 360 365 370 tcc ctg gaa aac gca gca cca cgc cca gtg tct cct ttc tac cca gcc 1267 Ser Leu Glu Asn Ala Ala Pro Arg Pro Val Ser Pro Phe Tyr Pro Ala 375 380 385 atc tcc aag gca atc cag gac aac gcc tac gca gcg ctt aac ggc aac 1315 Ile Ser Lys Ala Ile Gln Asp Asn Ala Tyr Ala Ala Leu Asn Gly Asn 390 395 400 405 gtc gac gtt gac cag gca acc acc gat atg aag gca gcg atc gaa aac 1363 Val Asp Val Asp Gln Ala Thr Thr Asp Met Lys Ala Ala Ile Glu Asn 410 415 420 gct tcc agc tagttcggta atttagttca ttctccggcc 1402 Ala Ser Ser <210> 140 <211> 424 <212> PRT <213> Corynebacterium glutamicum <400> 140 Met Ser Ser Ile Ser Arg Lys Thr Gly Ala Ser Leu Ala Ala Thr Thr 1 5 10 15 Leu Leu Ala Ala Ile Ala Leu Ala Gly Cys Ser Ser Asp Ser Ser Ser 20 25 30 Asp Ser Thr Asp Ser Thr Ala Ser Glu Gly Ala Asp Ser Arg Gly Pro 35 40 45 Ile Thr Phe Ala Met Gly Lys Asn Asp Thr Asp Lys Val Ile Pro Ile 50 55 60 Ile Asp Arg Trp Asn Glu Ala His Pro Asp Glu Gln Val Thr Leu Asn 65 70 75 80 Glu Leu Ala Gly Glu Ala Asp Ala Gln Arg Glu Thr Leu Val Gln Ser 85 90 95 Leu Gln Ala Gly Asn Ser Asp Tyr Asp Val Met Ala Leu Asp Val Ile 100 105 110 Trp Thr Ala Asp Phe Ala Ala Asn Gln Trp Leu Ala Pro Leu Glu Gly 115 120 125 Asp Leu Glu Val Asp Thr Ser Gly Leu Leu Gln Ser Thr Val Asp Ser 130 135 140 Ala Thr Tyr Asn Gly Thr Leu Tyr Ala Leu Pro Gln Asn Thr Asn Gly 145 150 155 160 Gln Leu Leu Phe Arg Asn Thr Glu Ile Ile Pro Glu Ala Pro Ala Asn 165 170 175 Trp Ala Asp Leu Val Glu Ser Cys Thr Leu Ala Glu Glu Ala Gly Val 180 185 190 Asp Cys Leu Thr Thr Gln Leu Lys Gln Tyr Glu Gly Leu Ser Val Asn 195 200 205 Thr Ile Gly Phe Ile Glu Gly Trp Gly Gly Ser Val Leu Asp Asp Asp 210 215 220 Gly Asn Val Thr Val Asp Ser Asp Asp Ala Lys Ala Gly Leu Gln Ala 225 230 235 240 Leu Val Asp Gly Phe Asp Asp Gly Thr Ile Ser Lys Ala Ser Leu Ala 245 250 255 Ala Thr Glu Glu Glu Thr Asn Leu Ala Phe Thr Glu Gly Gln Thr Ala 260 265 270 Tyr Ala Ile Asn Trp Pro Tyr Met Tyr Thr Asn Ser Glu Glu Ala Glu 275 280 285 Ala Thr Ala Gly Lys Phe Glu Val Gln Pro Leu Val Gly Lys Asp Gly 290 295 300 Val Gly Val Ser Thr Leu Gly Gly Tyr Asn Asn Gly Ile Asn Val Asn 305 310 315 320 Ser Glu Asn Lys Ala Thr Ala Arg Asp Phe Ile Glu Phe Ile Ile Asn 325 330 335 Glu Glu Asn Gln Thr Trp Phe Ala Asp Asn Ser Phe Pro Pro Val Leu 340 345 350 Ala Ser Ile Tyr Asp Asp Glu Ser Leu Val Glu Gln Tyr Pro Tyr Leu 355 360 365 Pro Ala Leu Lys Glu Ser Leu Glu Asn Ala Ala Pro Arg Pro Val Ser 370 375 380 Pro Phe Tyr Pro Ala Ile Ser Lys Ala Ile Gln Asp Asn Ala Tyr Ala 385 390 395 400 Ala Leu Asn Gly Asn Val Asp Val Asp Gln Ala Thr Thr Asp Met Lys 405 410 415 Ala Ala Ile Glu Asn Ala Ser Ser 420 <210> 141 <211> 901 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(871) <223> RXA03695 <400> 141 atagctacat acaatggaaa attacgtatg atgcgtctca cgctaacctg ataagaaagg 60 aaaaataagg gacaatcccc gcagaggggg tagccaagcg gtg tca gga gca gtg 115 Val Ser Gly Ala Val 1 5 ccc gag acg ccg tac cat caa cga tgg ctc gct ttc ccc gat gct gtg 163 Pro Glu Thr Pro Tyr His Gln Arg Trp Leu Ala Phe Pro Asp Ala Val 10 15 20 tcg tcc tac agg ccg tta gaa tcc aga ctc gcg ggc act agc cgc gcg 211 Ser Ser Tyr Arg Pro Leu Glu Ser Arg Leu Ala Gly Thr Ser Arg Ala 25 30 35 act gcc gac aac cgc acg ccg tgt acc gga agg ctt gga acg cct agg 259 Thr Ala Asp Asn Arg Thr Pro Cys Thr Gly Arg Leu Gly Thr Pro Arg 40 45 50 gtg ttc ctc atg gca aca gat gac gtt agc aag gcc tac agc tca ccg 307 Val Phe Leu Met Ala Thr Asp Asp Val Ser Lys Ala Tyr Ser Ser Pro 55 60 65 acc ttt gat gcg gag gca ctg ctc gga acg gta atc tcg gcc gag gac 355 Thr Phe Asp Ala Glu Ala Leu Leu Gly Thr Val Ile Ser Ala Glu Asp 70 75 80 85 ccc gac cgc gtg ctc atc gag ccc tgg gcg acc ggc gtc gac ggg gtg 403 Pro Asp Arg Val Leu Ile Glu Pro Trp Ala Thr Gly Val Asp Gly Val 90 95 100 atc ctc gat gtg ggc tct ggt acc ggg cgg tgg acc ggt cac ctc gcg 451 Ile Leu Asp Val Gly Ser Gly Thr Gly Arg Trp Thr Gly His Leu Ala 105 110 115 agc ctc gga cac cag atc gag gga ctg gaa cca gcg aca cgg ctc gta 499 Ser Leu Gly His Gln Ile Glu Gly Leu Glu Pro Ala Thr Arg Leu Val 120 125 130 gag ctc gct cgt cag aca cat ccc tca gtg acc ttt cac cac ggc act 547 Glu Leu Ala Arg Gln Thr His Pro Ser Val Thr Phe His His Gly Thr 135 140 145 att acc gac ctc tca gac tcg ccg aag cgc tgg gca ggc ctg ttg gcc 595 Ile Thr Asp Leu Ser Asp Ser Pro Lys Arg Trp Ala Gly Leu Leu Ala 150 155 160 165 tgg tac tcc ctg atc cac atg ggc ccg ggc gag ctg ccc gac gcc ctg 643 Trp Tyr Ser Leu Ile His Met Gly Pro Gly Glu Leu Pro Asp Ala Leu 170 175 180 gtc gca ctg cgg atg gcg gtg gag gat ggt ggt ggc ctg ctg atg tcc 691 Val Ala Leu Arg Met Ala Val Glu Asp Gly Gly Gly Leu Leu Met Ser 185 190 195 ttt ttc tcc ggg cct tct ctg gaa ccg atg tat cat ccc gtg gcc acc 739 Phe Phe Ser Gly Pro Ser Leu Glu Pro Met Tyr His Pro Val Ala Thr 200 205 210 gcc tac cgg tgg cca ctg ccc gag ctt gcc cag gca ctg gag acc gcg 787 Ala Tyr Arg Trp Pro Leu Pro Glu Leu Ala Gln Ala Leu Glu Thr Ala 215 220 225 ggt ttc cag gtc acc agt agt cac tgg gat cct cgg ttc ccc cat gcc 835 Gly Phe Gln Val Thr Ser Ser His Trp Asp Pro Arg Phe Pro His Ala 230 235 240 245 tat ctc acc gcc gag gcc agc aag cag aat ctc gcc tgacagtggt 881 Tyr Leu Thr Ala Glu Ala Ser Lys Gln Asn Leu Ala 250 255 ggcctactcg gtagtcgtct 901 <210> 142 <211> 257 <212> PRT <213> Corynebacterium glutamicum <400> 142 Val Ser Gly Ala Val Pro Glu Thr Pro Tyr His Gln Arg Trp Leu Ala 1 5 10 15 Phe Pro Asp Ala Val Ser Ser Tyr Arg Pro Leu Glu Ser Arg Leu Ala 20 25 30 Gly Thr Ser Arg Ala Thr Ala Asp Asn Arg Thr Pro Cys Thr Gly Arg 35 40 45 Leu Gly Thr Pro Arg Val Phe Leu Met Ala Thr Asp Asp Val Ser Lys 50 55 60 Ala Tyr Ser Ser Pro Thr Phe Asp Ala Glu Ala Leu Leu Gly Thr Val 65 70 75 80 Ile Ser Ala Glu Asp Pro Asp Arg Val Leu Ile Glu Pro Trp Ala Thr 85 90 95 Gly Val Asp Gly Val Ile Leu Asp Val Gly Ser Gly Thr Gly Arg Trp 100 105 110 Thr Gly His Leu Ala Ser Leu Gly His Gln Ile Glu Gly Leu Glu Pro 115 120 125 Ala Thr Arg Leu Val Glu Leu Ala Arg Gln Thr His Pro Ser Val Thr 130 135 140 Phe His His Gly Thr Ile Thr Asp Leu Ser Asp Ser Pro Lys Arg Trp 145 150 155 160 Ala Gly Leu Leu Ala Trp Tyr Ser Leu Ile His Met Gly Pro Gly Glu 165 170 175 Leu Pro Asp Ala Leu Val Ala Leu Arg Met Ala Val Glu Asp Gly Gly 180 185 190 Gly Leu Leu Met Ser Phe Phe Ser Gly Pro Ser Leu Glu Pro Met Tyr 195 200 205 His Pro Val Ala Thr Ala Tyr Arg Trp Pro Leu Pro Glu Leu Ala Gln 210 215 220 Ala Leu Glu Thr Ala Gly Phe Gln Val Thr Ser Ser His Trp Asp Pro 225 230 235 240 Arg Phe Pro His Ala Tyr Leu Thr Ala Glu Ala Ser Lys Gln Asn Leu 245 250 255 Ala <210> 143 <211> 1582 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1552) <223> RXA03754 <400> 143 tttgggcggg aggtgacatt tatgcctctt aatagctata ctgtcccatg aaacgatatt 60 ttaatctcat atagtgagat ttacagattt ttaaaggacg gtg agt tcc atg acc 115 Val Ser Ser Met Thr 1 5 agc ccc gtg gag aac agc acc tca act gag aag ctg acc ctg gca gag 163 Ser Pro Val Glu Asn Ser Thr Ser Thr Glu Lys Leu Thr Leu Ala Glu 10 15 20 aag gtg tgg cgc gac cat gtc gtg tcc aag gga gaa aac ggc gag ccc 211 Lys Val Trp Arg Asp His Val Val Ser Lys Gly Glu Asn Gly Glu Pro 25 30 35 gac ctc ctc tac atc gac ctg cag ctg ctg cat gaa gtg acc tca cca 259 Asp Leu Leu Tyr Ile Asp Leu Gln Leu Leu His Glu Val Thr Ser Pro 40 45 50 cag gca ttt gac ggc ctg cgc atg acc ggc cgt aaa ctg cgc cac cca 307 Gln Ala Phe Asp Gly Leu Arg Met Thr Gly Arg Lys Leu Arg His Pro 55 60 65 gaa ctg cac ctg gcc acc gaa gac cac aac gtg cca acc gaa ggc atc 355 Glu Leu His Leu Ala Thr Glu Asp His Asn Val Pro Thr Glu Gly Ile 70 75 80 85 aag act ggc tca ctg ctg gaa atc aac gac aag att tcc cgc ctg cag 403 Lys Thr Gly Ser Leu Leu Glu Ile Asn Asp Lys Ile Ser Arg Leu Gln 90 95 100 gta tct act ctg cgc gac aac tgt gaa gaa ttc ggc gtg cgc ctg cac 451 Val Ser Thr Leu Arg Asp Asn Cys Glu Glu Phe Gly Val Arg Leu His 105 110 115 cca atg ggt gat gtc cga cag ggc atc gtg cac acc gtc ggc cca cag 499 Pro Met Gly Asp Val Arg Gln Gly Ile Val His Thr Val Gly Pro Gln 120 125 130 ctc ggc gca acc cag cca ggc atg acc att gtg tgc ggt gac tcc cac 547 Leu Gly Ala Thr Gln Pro Gly Met Thr Ile Val Cys Gly Asp Ser His 135 140 145 acc tcc acc cac ggt gct ttt ggc tcc atg gca ttc ggc atc ggt acc 595 Thr Ser Thr His Gly Ala Phe Gly Ser Met Ala Phe Gly Ile Gly Thr 150 155 160 165 tca gag gtt gag cac gtc atg gct act caa acc ctg cca ctg aag cct 643 Ser Glu Val Glu His Val Met Ala Thr Gln Thr Leu Pro Leu Lys Pro 170 175 180 ttc aag acc atg gcc att gaa gtt act ggt gaa ctg cag cca ggt gtt 691 Phe Lys Thr Met Ala Ile Glu Val Thr Gly Glu Leu Gln Pro Gly Val 185 190 195 tcc tcc aag gac ctg att ctg gcg att atc gcc aag atc ggc acc ggc 739 Ser Ser Lys Asp Leu Ile Leu Ala Ile Ile Ala Lys Ile Gly Thr Gly 200 205 210 ggc gga cag ggc tac gtt ctg gaa tac cgc ggc gaa gca atc cgt aag 787 Gly Gly Gln Gly Tyr Val Leu Glu Tyr Arg Gly Glu Ala Ile Arg Lys 215 220 225 atg tcc atg gat gca cgc atg acc atg tgc aac atg tcc atc gaa gct 835 Met Ser Met Asp Ala Arg Met Thr Met Cys Asn Met Ser Ile Glu Ala 230 235 240 245 ggc gca cgt gcc ggc atg atc gcc cca gac caa acc acc ttc gac tac 883 Gly Ala Arg Ala Gly Met Ile Ala Pro Asp Gln Thr Thr Phe Asp Tyr 250 255 260 gtt gaa ggc cgc gaa atg gca cca aag ggc gcc gac tgg gac gaa gca 931 Val Glu Gly Arg Glu Met Ala Pro Lys Gly Ala Asp Trp Asp Glu Ala 265 270 275 gtt gct tac tgg aag acc ctg cca acc gac gaa ggc gca acc ttt gac 979 Val Ala Tyr Trp Lys Thr Leu Pro Thr Asp Glu Gly Ala Thr Phe Asp 280 285 290 aag gtc gta gaa atc gat ggc tcg gca ctg acc cca ttc atc acc tgg 1027 Lys Val Val Glu Ile Asp Gly Ser Ala Leu Thr Pro Phe Ile Thr Trp 295 300 305 ggc acc aac cca ggc cag ggc ctg cca ctg ggc gaa tcc gta cca agc 1075 Gly Thr Asn Pro Gly Gln Gly Leu Pro Leu Gly Glu Ser Val Pro Ser 310 315 320 325 cca gaa gac ttc acc aac gac aac gac aag gca gca gcc gaa aag gca 1123 Pro Glu Asp Phe Thr Asn Asp Asn Asp Lys Ala Ala Ala Glu Lys Ala 330 335 340 ctg cag tac atg gac ctg gta cca gga acc cca ctg cgc gac atc aag 1171 Leu Gln Tyr Met Asp Leu Val Pro Gly Thr Pro Leu Arg Asp Ile Lys 345 350 355 atc gac acc gtc ttc ctg gga tcc tgc acc aac gcc cgc atc gaa gac 1219 Ile Asp Thr Val Phe Leu Gly Ser Cys Thr Asn Ala Arg Ile Glu Asp 360 365 370 ctg cag atc gcc gct gac atc ctc aag ggc cac aaa atc gcc gac ggc 1267 Leu Gln Ile Ala Ala Asp Ile Leu Lys Gly His Lys Ile Ala Asp Gly 375 380 385 atg cgc atg atg gtc gtg cct tcc tcc acc tgg atc aag caa gag gca 1315 Met Arg Met Met Val Val Pro Ser Ser Thr Trp Ile Lys Gln Glu Ala 390 395 400 405 gaa gcg ctc gga ctg gac aaa atc ttc acc gac gct ggc gct gaa tgg 1363 Glu Ala Leu Gly Leu Asp Lys Ile Phe Thr Asp Ala Gly Ala Glu Trp 410 415 420 cgt acc gca ggc tgc tcc atg tgc ctg ggc atg aac cca gac caa ctg 1411 Arg Thr Ala Gly Cys Ser Met Cys Leu Gly Met Asn Pro Asp Gln Leu 425 430 435 aag cca ggc gag cgc tcc gca tcc acc tcc aac cga aac ttc gaa gga 1459 Lys Pro Gly Glu Arg Ser Ala Ser Thr Ser Asn Arg Asn Phe Glu Gly 440 445 450 cgc caa gga cca gga ggc cgc acc cac ctg gta tcc cca gca gtc gca 1507 Arg Gln Gly Pro Gly Gly Arg Thr His Leu Val Ser Pro Ala Val Ala 455 460 465 gcc gcc acc gca atc cgc ggc acc ctg tcc tca cct gca gat atc 1552 Ala Ala Thr Ala Ile Arg Gly Thr Leu Ser Ser Pro Ala Asp Ile 470 475 480 taaggaaggc tagaaaacaa tggaaaaatt 1582 <210> 144 <211> 484 <212> PRT <213> Corynebacterium glutamicum <400> 144 Val Ser Ser Met Thr Ser Pro Val Glu Asn Ser Thr Ser Thr Glu Lys 1 5 10 15 Leu Thr Leu Ala Glu Lys Val Trp Arg Asp His Val Val Ser Lys Gly 20 25 30 Glu Asn Gly Glu Pro Asp Leu Leu Tyr Ile Asp Leu Gln Leu Leu His 35 40 45 Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Met Thr Gly Arg 50 55 60 Lys Leu Arg His Pro Glu Leu His Leu Ala Thr Glu Asp His Asn Val 65 70 75 80 Pro Thr Glu Gly Ile Lys Thr Gly Ser Leu Leu Glu Ile Asn Asp Lys 85 90 95 Ile Ser Arg Leu Gln Val Ser Thr Leu Arg Asp Asn Cys Glu Glu Phe 100 105 110 Gly Val Arg Leu His Pro Met Gly Asp Val Arg Gln Gly Ile Val His 115 120 125 Thr Val Gly Pro Gln Leu Gly Ala Thr Gln Pro Gly Met Thr Ile Val 130 135 140 Cys Gly Asp Ser His Thr Ser Thr His Gly Ala Phe Gly Ser Met Ala 145 150 155 160 Phe Gly Ile Gly Thr Ser Glu Val Glu His Val Met Ala Thr Gln Thr 165 170 175 Leu Pro Leu Lys Pro Phe Lys Thr Met Ala Ile Glu Val Thr Gly Glu 180 185 190 Leu Gln Pro Gly Val Ser Ser Lys Asp Leu Ile Leu Ala Ile Ile Ala 195 200 205 Lys Ile Gly Thr Gly Gly Gly Gln Gly Tyr Val Leu Glu Tyr Arg Gly 210 215 220 Glu Ala Ile Arg Lys Met Ser Met Asp Ala Arg Met Thr Met Cys Asn 225 230 235 240 Met Ser Ile Glu Ala Gly Ala Arg Ala Gly Met Ile Ala Pro Asp Gln 245 250 255 Thr Thr Phe Asp Tyr Val Glu Gly Arg Glu Met Ala Pro Lys Gly Ala 260 265 270 Asp Trp Asp Glu Ala Val Ala Tyr Trp Lys Thr Leu Pro Thr Asp Glu 275 280 285 Gly Ala Thr Phe Asp Lys Val Val Glu Ile Asp Gly Ser Ala Leu Thr 290 295 300 Pro Phe Ile Thr Trp Gly Thr Asn Pro Gly Gln Gly Leu Pro Leu Gly 305 310 315 320 Glu Ser Val Pro Ser Pro Glu Asp Phe Thr Asn Asp Asn Asp Lys Ala 325 330 335 Ala Ala Glu Lys Ala Leu Gln Tyr Met Asp Leu Val Pro Gly Thr Pro 340 345 350 Leu Arg Asp Ile Lys Ile Asp Thr Val Phe Leu Gly Ser Cys Thr Asn 355 360 365 Ala Arg Ile Glu Asp Leu Gln Ile Ala Ala Asp Ile Leu Lys Gly His 370 375 380 Lys Ile Ala Asp Gly Met Arg Met Met Val Val Pro Ser Ser Thr Trp 385 390 395 400 Ile Lys Gln Glu Ala Glu Ala Leu Gly Leu Asp Lys Ile Phe Thr Asp 405 410 415 Ala Gly Ala Glu Trp Arg Thr Ala Gly Cys Ser Met Cys Leu Gly Met 420 425 430 Asn Pro Asp Gln Leu Lys Pro Gly Glu Arg Ser Ala Ser Thr Ser Asn 435 440 445 Arg Asn Phe Glu Gly Arg Gln Gly Pro Gly Gly Arg Thr His Leu Val 450 455 460 Ser Pro Ala Val Ala Ala Ala Thr Ala Ile Arg Gly Thr Leu Ser Ser 465 470 475 480 Pro Ala Asp Ile <210> 145 <211> 709 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(679) <223> RXA03829 <400> 145 cgaccttgtc ctagctctac gcaatgccca aaatcccctc cctcttgcag gtcgtggcac 60 acgcgaagca cagcaaagca tcctggaatt agcagatctt ttg gca gct gat gta 115 Leu Ala Ala Asp Val 1 5 gcc acc agc gcg ccc gcc cga gga ttc ttc cat ccc tca gga aac tct 163 Ala Thr Ser Ala Pro Ala Arg Gly Phe Phe His Pro Ser Gly Asn Ser 10 15 20 cgc gac ctg gtc gta tgt gga ggt ttc gct gcg acc ggt gca gct tca 211 Arg Asp Leu Val Val Cys Gly Gly Phe Ala Ala Thr Gly Ala Ala Ser 25 30 35 gaa ata cgc cga gcc aac gtg gcg ctt gtg ctg ggc gcc ggt ttg aac 259 Glu Ile Arg Arg Ala Asn Val Ala Leu Val Leu Gly Ala Gly Leu Asn 40 45 50 caa ttc acc atg gca ttc ggg gag gct ttc ggt gaa ctc gcg gaa gtg 307 Gln Phe Thr Met Ala Phe Gly Glu Ala Phe Gly Glu Leu Ala Glu Val 55 60 65 ctc caa gtg gat ctt gaa aca cag acc acc aac ccg cgc att aac cat 355 Leu Gln Val Asp Leu Glu Thr Gln Thr Thr Asn Pro Arg Ile Asn His 70 75 80 85 ttc att agc gca gat aac aca act gtt gta gcc gca gtg ctt tta aag 403 Phe Ile Ser Ala Asp Asn Thr Thr Val Val Ala Ala Val Leu Leu Lys 90 95 100 ctt cgt aca caa aat ttc cac gca ccg cgc cgg cta tac ctg gac gat 451 Leu Arg Thr Gln Asn Phe His Ala Pro Arg Arg Leu Tyr Leu Asp Asp 105 110 115 aat cct ctg aca tgc cct ggc ggc gac gcc ctt gca gcc gac ggc cga 499 Asn Pro Leu Thr Cys Pro Gly Gly Asp Ala Leu Ala Ala Asp Gly Arg 120 125 130 ctc gat cca cac agc ctc atg cgc caa ctt aac ggt att ttg cca gct 547 Leu Asp Pro His Ser Leu Met Arg Gln Leu Asn Gly Ile Leu Pro Ala 135 140 145 aac aag ttc gtc gcc tcc gat ggc gga cac ttc atc gga ggg gcc aac 595 Asn Lys Phe Val Ala Ser Asp Gly Gly His Phe Ile Gly Gly Ala Asn 150 155 160 165 acc tac ttc gac ctg gaa tca cga gac agc atc gtg ctt ttg gga acc 643 Thr Tyr Phe Asp Leu Glu Ser Arg Asp Ser Ile Val Leu Leu Gly Thr 170 175 180 gcc ttc aat cca tcg gcc tcg gct tcc cca ccg ccg taggtgtcgc 689 Ala Phe Asn Pro Ser Ala Ser Ala Ser Pro Pro Pro 185 190 cacagctgct gggcaagagt 709 <210> 146 <211> 193 <212> PRT <213> Corynebacterium glutamicum <400> 146 Leu Ala Ala Asp Val Ala Thr Ser Ala Pro Ala Arg Gly Phe Phe His 1 5 10 15 Pro Ser Gly Asn Ser Arg Asp Leu Val Val Cys Gly Gly Phe Ala Ala 20 25 30 Thr Gly Ala Ala Ser Glu Ile Arg Arg Ala Asn Val Ala Leu Val Leu 35 40 45 Gly Ala Gly Leu Asn Gln Phe Thr Met Ala Phe Gly Glu Ala Phe Gly 50 55 60 Glu Leu Ala Glu Val Leu Gln Val Asp Leu Glu Thr Gln Thr Thr Asn 65 70 75 80 Pro Arg Ile Asn His Phe Ile Ser Ala Asp Asn Thr Thr Val Val Ala 85 90 95 Ala Val Leu Leu Lys Leu Arg Thr Gln Asn Phe His Ala Pro Arg Arg 100 105 110 Leu Tyr Leu Asp Asp Asn Pro Leu Thr Cys Pro Gly Gly Asp Ala Leu 115 120 125 Ala Ala Asp Gly Arg Leu Asp Pro His Ser Leu Met Arg Gln Leu Asn 130 135 140 Gly Ile Leu Pro Ala Asn Lys Phe Val Ala Ser Asp Gly Gly His Phe 145 150 155 160 Ile Gly Gly Ala Asn Thr Tyr Phe Asp Leu Glu Ser Arg Asp Ser Ile 165 170 175 Val Leu Leu Gly Thr Ala Phe Asn Pro Ser Ala Ser Ala Ser Pro Pro 180 185 190 Pro <210> 147 <211> 1315 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1285) <223> RXA03871 <400> 147 taaagccttg ggagcggtga ttatgctttt ggctgtctat gtcctcatca ttggagccat 60 cggagcgtta cgattgtttt ccaaggtgag aaaggtttaa ttg atg tct cgt tcg 115 Leu Met Ser Arg Ser 1 5 ccg gag caa cat ttg gca gaa att tca gcg ctg ctt ccc cca caa aag 163 Pro Glu Gln His Leu Ala Glu Ile Ser Ala Leu Leu Pro Pro Gln Lys 10 15 20 tcc acg ttc gtg aat ctg cgc gaa gcg ttg gga cgc cgc acg ttt tca 211 Ser Thr Phe Val Asn Leu Arg Glu Ala Leu Gly Arg Arg Thr Phe Ser 25 30 35 gcg gtc act gcg cag tgg gat tcg cca cgt ttt gat aat tcc caa atg 259 Ala Val Thr Ala Gln Trp Asp Ser Pro Arg Phe Asp Asn Ser Gln Met 40 45 50 gat ggc ttc gcg ctt ggc ccc tca cat ctt aac ggt ggc acc ttc gca 307 Asp Gly Phe Ala Leu Gly Pro Ser His Leu Asn Gly Gly Thr Phe Ala 55 60 65 gtc ggt cca acc att ccc gct ggt cat gat cct gat cag tgg tac cca 355 Val Gly Pro Thr Ile Pro Ala Gly His Asp Pro Asp Gln Trp Tyr Pro 70 75 80 85 cga ggc atc gaa aaa gac atc gcg ccg att atg acg ggt gcg cgc ctt 403 Arg Gly Ile Glu Lys Asp Ile Ala Pro Ile Met Thr Gly Ala Arg Leu 90 95 100 cct aaa aac acc gcc gcg atc att cct gtg gag aaa acc aca ccg gga 451 Pro Lys Asn Thr Ala Ala Ile Ile Pro Val Glu Lys Thr Thr Pro Gly 105 110 115 aat ttc gac gcc cca cag gta gaa atc ccc gcc acc ccg caa ggt cag 499 Asn Phe Asp Ala Pro Gln Val Glu Ile Pro Ala Thr Pro Gln Gly Gln 120 125 130 ttc ata cgg ttg cag ggt tcg gat att act gcc ggc gac gag atc att 547 Phe Ile Arg Leu Gln Gly Ser Asp Ile Thr Ala Gly Asp Glu Ile Ile 135 140 145 cca gca ggt acg gag ctt aac tcg gtg cac atc ggg gtg ttg gct agt 595 Pro Ala Gly Thr Glu Leu Asn Ser Val His Ile Gly Val Leu Ala Ser 150 155 160 165 cag tcg atc aag agc att gaa gtc gca gca aag cca cgt gtc ctc atc 643 Gln Ser Ile Lys Ser Ile Glu Val Ala Ala Lys Pro Arg Val Leu Ile 170 175 180 atc acc ggc ggg tct gaa att tca gaa cag cac gga ccc gcc acg atc 691 Ile Thr Gly Gly Ser Glu Ile Ser Glu Gln His Gly Pro Ala Thr Ile 185 190 195 cct gat gcc aac ggc cct ctg ctt cgt tcc ctg tgc gcc cgc aac aat 739 Pro Asp Ala Asn Gly Pro Leu Leu Arg Ser Leu Cys Ala Arg Asn Asn 200 205 210 atc gag gtc atc gcg gga ctg cac acc aac gac gat cct gaa cga ctc 787 Ile Glu Val Ile Ala Gly Leu His Thr Asn Asp Asp Pro Glu Arg Leu 215 220 225 cgc ttt gaa ctg gaa aac gcc att gac cag tat caa ccg gat gtc atc 835 Arg Phe Glu Leu Glu Asn Ala Ile Asp Gln Tyr Gln Pro Asp Val Ile 230 235 240 245 atc acc tct ggt ggt atc agc cac ggt aaa ttt gag gtg ttt agg cag 883 Ile Thr Ser Gly Gly Ile Ser His Gly Lys Phe Glu Val Phe Arg Gln 250 255 260 atc ctc gaa ggc acc ccg aac tcc tgg ttt gga cat gtc gat cag cag 931 Ile Leu Glu Gly Thr Pro Asn Ser Trp Phe Gly His Val Asp Gln Gln 265 270 275 cct ggc ggt cct caa ggc atc tcc act ttt gct gaa act cct gtc att 979 Pro Gly Gly Pro Gln Gly Ile Ser Thr Phe Ala Glu Thr Pro Val Ile 280 285 290 tca ctt ccc gga aat ccg att tcc acc ttg gtg agt ttc aca ctt ttc 1027 Ser Leu Pro Gly Asn Pro Ile Ser Thr Leu Val Ser Phe Thr Leu Phe 295 300 305 gtc gcg cca gcg ctc aac cgc cag ccg ctc cgc cac ctc gat gcc cgc 1075 Val Ala Pro Ala Leu Asn Arg Gln Pro Leu Arg His Leu Asp Ala Arg 310 315 320 325 atc acc gct ccg gtc cag ggc ttg caa gac aat cgc gag caa ttc ctt 1123 Ile Thr Ala Pro Val Gln Gly Leu Gln Asp Asn Arg Glu Gln Phe Leu 330 335 340 cgc ggc acc atc agt tac cgc aac ggc cac gtc ctc gcc acg cct ctc 1171 Arg Gly Thr Ile Ser Tyr Arg Asn Gly His Val Leu Ala Thr Pro Leu 345 350 355 ctg ggc acc agt tcc cac ctg ctg gtt caa gct gcc acc gca gac tgt 1219 Leu Gly Thr Ser Ser His Leu Leu Val Gln Ala Ala Thr Ala Asp Cys 360 365 370 ctg atc agg atc ccg gcg cgg act acg gtg gag gaa aac gac atc gtt 1267 Leu Ile Arg Ile Pro Ala Arg Thr Thr Val Glu Glu Asn Asp Ile Val 375 380 385 aag att tac cca ttc aac taacaggagt taatttaatg agcgagctca 1315 Lys Ile Tyr Pro Phe Asn 390 395 <210> 148 <211> 395 <212> PRT <213> Corynebacterium glutamicum <400> 148 Leu Met Ser Arg Ser Pro Glu Gln His Leu Ala Glu Ile Ser Ala Leu 1 5 10 15 Leu Pro Pro Gln Lys Ser Thr Phe Val Asn Leu Arg Glu Ala Leu Gly 20 25 30 Arg Arg Thr Phe Ser Ala Val Thr Ala Gln Trp Asp Ser Pro Arg Phe 35 40 45 Asp Asn Ser Gln Met Asp Gly Phe Ala Leu Gly Pro Ser His Leu Asn 50 55 60 Gly Gly Thr Phe Ala Val Gly Pro Thr Ile Pro Ala Gly His Asp Pro 65 70 75 80 Asp Gln Trp Tyr Pro Arg Gly Ile Glu Lys Asp Ile Ala Pro Ile Met 85 90 95 Thr Gly Ala Arg Leu Pro Lys Asn Thr Ala Ala Ile Ile Pro Val Glu 100 105 110 Lys Thr Thr Pro Gly Asn Phe Asp Ala Pro Gln Val Glu Ile Pro Ala 115 120 125 Thr Pro Gln Gly Gln Phe Ile Arg Leu Gln Gly Ser Asp Ile Thr Ala 130 135 140 Gly Asp Glu Ile Ile Pro Ala Gly Thr Glu Leu Asn Ser Val His Ile 145 150 155 160 Gly Val Leu Ala Ser Gln Ser Ile Lys Ser Ile Glu Val Ala Ala Lys 165 170 175 Pro Arg Val Leu Ile Ile Thr Gly Gly Ser Glu Ile Ser Glu Gln His 180 185 190 Gly Pro Ala Thr Ile Pro Asp Ala Asn Gly Pro Leu Leu Arg Ser Leu 195 200 205 Cys Ala Arg Asn Asn Ile Glu Val Ile Ala Gly Leu His Thr Asn Asp 210 215 220 Asp Pro Glu Arg Leu Arg Phe Glu Leu Glu Asn Ala Ile Asp Gln Tyr 225 230 235 240 Gln Pro Asp Val Ile Ile Thr Ser Gly Gly Ile Ser His Gly Lys Phe 245 250 255 Glu Val Phe Arg Gln Ile Leu Glu Gly Thr Pro Asn Ser Trp Phe Gly 260 265 270 His Val Asp Gln Gln Pro Gly Gly Pro Gln Gly Ile Ser Thr Phe Ala 275 280 285 Glu Thr Pro Val Ile Ser Leu Pro Gly Asn Pro Ile Ser Thr Leu Val 290 295 300 Ser Phe Thr Leu Phe Val Ala Pro Ala Leu Asn Arg Gln Pro Leu Arg 305 310 315 320 His Leu Asp Ala Arg Ile Thr Ala Pro Val Gln Gly Leu Gln Asp Asn 325 330 335 Arg Glu Gln Phe Leu Arg Gly Thr Ile Ser Tyr Arg Asn Gly His Val 340 345 350 Leu Ala Thr Pro Leu Leu Gly Thr Ser Ser His Leu Leu Val Gln Ala 355 360 365 Ala Thr Ala Asp Cys Leu Ile Arg Ile Pro Ala Arg Thr Thr Val Glu 370 375 380 Glu Asn Asp Ile Val Lys Ile Tyr Pro Phe Asn 385 390 395 <210> 149 <211> 2101 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(2071) <223> RXA04120 <400> 149 caacgaaaca aatgcaagcc cccaatcatg ggtttctacc aattaaattt tctttcagaa 60 aatatctccc cacataaaag ttccttgata ggctcgagag atg aaa gtg acc caa 115 Met Lys Val Thr Gln 1 5 agc aca ttc ctt aaa tcg gta gct gcg ttc act gtc gca gcc tta acc 163 Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr Val Ala Ala Leu Thr 10 15 20 ctg acc atc tct tcg tgt tcc agc ggt gaa gac acc tcc gca agc tcc 211 Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp Thr Ser Ala Ser Ser 25 30 35 acg gat act gaa aac tcc tca acc caa gca gca gcg tct ccc cca ctt 259 Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala Ala Ser Pro Pro Leu 40 45 50 gcg cct tgt gaa ctt ccc gcc gac gct tct gct gaa gag gaa gta gaa 307 Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala Glu Glu Glu Val Glu 55 60 65 ggc act cac aca ggt gaa gat att tct gtt gcc ccg gaa atc ggt acc 355 Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala Pro Glu Ile Gly Thr 70 75 80 85 ggc tac cgc gag ggc atg acc cct gtt caa acc caa ggt tat gcg gtg 403 Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr Gln Gly Tyr Ala Val 90 95 100 gca act gca aac ccc atc gct tct gaa gca gcc tgc gcg gtg tta aga 451 Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala Cys Ala Val Leu Arg 105 110 115 gaa ggc ggc act gca gct gat gct ctt gtc acc gcg cag ttt gtt ttg 499 Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr Ala Gln Phe Val Leu 120 125 130 gga ctg acg gaa ccg cag tcg tct ggc ctt ggt ggt ggc gga tac att 547 Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly Gly Gly Gly Tyr Ile 135 140 145 ctg tac tac gac gcc gaa gcc aat gcg gtg aca gcc att gat ggc cgt 595 Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr Ala Ile Asp Gly Arg 150 155 160 165 gaa aca gcg cca gtt gct gct gat gaa aac tat ctc att cat gtt tct 643 Glu Thr Ala Pro Val Ala Ala Asp Glu Asn Tyr Leu Ile His Val Ser 170 175 180 gca gag gat caa acg gca cct gtt cct gat gcc cga cgt tcc ggc agg 691 Ala Glu Asp Gln Thr Ala Pro Val Pro Asp Ala Arg Arg Ser Gly Arg 185 190 195 tca att ggt gtg cca gga atc gtg gca gcc ctt gga cag ctg cat gat 739 Ser Ile Gly Val Pro Gly Ile Val Ala Ala Leu Gly Gln Leu His Asp 200 205 210 tca ttc gga aag acc tcc tgg cag gac gtg ctg aca act ccg cag cag 787 Ser Phe Gly Lys Thr Ser Trp Gln Asp Val Leu Thr Thr Pro Gln Gln 215 220 225 ctc gca act gat ggt ttt tcc atc agc cct cgc atg tca gca tca att 835 Leu Ala Thr Asp Gly Phe Ser Ile Ser Pro Arg Met Ser Ala Ser Ile 230 235 240 245 gct aac tcc gct gag gat ctc tcc cac gat ccg gaa gct gcc gca tat 883 Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro Glu Ala Ala Ala Tyr 250 255 260 ttc ctt gat gaa aac ggt gat gcg aag gca ccc ggc aca ctt tta caa 931 Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro Gly Thr Leu Leu Gln 265 270 275 aac cct gac tat gca gaa acg att cgt ctc atc tct gaa ggt ggc ccc 979 Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile Ser Glu Gly Gly Pro 280 285 290 gat gcg ttc tac acg ggt gag att gca gca gac atc gtg gaa cgc gcc 1027 Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp Ile Val Glu Arg Ala 295 300 305 acc cgt gag gtt gac ggt ttc aca cca tca ctg atg agc acg gca gat 1075 Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu Met Ser Thr Ala Asp 310 315 320 325 ttg gct gcc tac act ccg gaa act cgt gaa gct ttg tgt gct ccc tac 1123 Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala Leu Cys Ala Pro Tyr 330 335 340 cgc gac aag att gtt tgt ggc atg cca ccg tca tca tcg ggt ggc gtc 1171 Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser Ser Ser Gly Gly Val 345 350 355 aca gtg atg gaa acc ctg ggt atc ttg aac aac ttt gat ctc gcc caa 1219 Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn Phe Asp Leu Ala Gln 360 365 370 tac cca ccc act gag gtt ggt ttg gat ggc gga ttg cca aat gcg gaa 1267 Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly Leu Pro Asn Ala Glu 375 380 385 gct gtt cac ctg att tca gag gct gag cgc ctg gct tat gct gat cgc 1315 Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu Ala Tyr Ala Asp Arg 390 395 400 405 gat gct tac atc ggt gat cct gct ttc gtg gaa gtt cca gca ggt ggt 1363 Asp Ala Tyr Ile Gly Asp Pro Ala Phe Val Glu Val Pro Ala Gly Gly 410 415 420 gtc gaa gag ttg atc agc gat gtc tac acg ggt gaa cgc tca gaa ctt 1411 Val Glu Glu Leu Ile Ser Asp Val Tyr Thr Gly Glu Arg Ser Glu Leu 425 430 435 att gat cca gag cat tca atg ggt cag gca act gct ggt ctg agc cag 1459 Ile Asp Pro Glu His Ser Met Gly Gln Ala Thr Ala Gly Leu Ser Gln 440 445 450 gaa cca gtc atg gct gcc ctg ccg gaa agt ggc acc agc cat att tcc 1507 Glu Pro Val Met Ala Ala Leu Pro Glu Ser Gly Thr Ser His Ile Ser 455 460 465 atc atc gat tcc tat ggc aac gca gca tcg ttg acc acc agt gtg gaa 1555 Ile Ile Asp Ser Tyr Gly Asn Ala Ala Ser Leu Thr Thr Ser Val Glu 470 475 480 485 gct gct ttc ggt tcc ttc cac ttc acc cgt ggt ttc att ttg aat aat 1603 Ala Ala Phe Gly Ser Phe His Phe Thr Arg Gly Phe Ile Leu Asn Asn 490 495 500 cag ctg aca gat ttc tcc gct gaa cca ctt gat gag gac ggc gag ccc 1651 Gln Leu Thr Asp Phe Ser Ala Glu Pro Leu Asp Glu Asp Gly Glu Pro 505 510 515 gtg gcc aat cgt gtc gag tca gca aag cgc cca cgg tct tcc atg tcg 1699 Val Ala Asn Arg Val Glu Ser Ala Lys Arg Pro Arg Ser Ser Met Ser 520 525 530 cca atg cta gtg ttc aac gcc agt ggc gat ggt gaa atc gcg gat ctg 1747 Pro Met Leu Val Phe Asn Ala Ser Gly Asp Gly Glu Ile Ala Asp Leu 535 540 545 aat atg gtg ctg ggc tcc cct ggc gga tcc ttg att att cag tac gtg 1795 Asn Met Val Leu Gly Ser Pro Gly Gly Ser Leu Ile Ile Gln Tyr Val 550 555 560 565 gtg aaa acc ctg gtc aac atc atc gac tgg gat atg gat cca cag cag 1843 Val Lys Thr Leu Val Asn Ile Ile Asp Trp Asp Met Asp Pro Gln Gln 570 575 580 gca gtg tct gcg ccc aac ttt ggt gcg atg aac cag cct aag act gga 1891 Ala Val Ser Ala Pro Asn Phe Gly Ala Met Asn Gln Pro Lys Thr Gly 585 590 595 ctg gga agc gag cat ccg ctg atc gcc aat gat tca gca gag ctt gta 1939 Leu Gly Ser Glu His Pro Leu Ile Ala Asn Asp Ser Ala Glu Leu Val 600 605 610 tct gaa ctg gaa agc aaa ggc cac gaa gtt aat gtg ggc gag caa tcc 1987 Ser Glu Leu Glu Ser Lys Gly His Glu Val Asn Val Gly Glu Gln Ser 615 620 625 agt ggc cta tcg gcg ttg gtg aaa aac ggc gac acc att gtc ggt ggc 2035 Ser Gly Leu Ser Ala Leu Val Lys Asn Gly Asp Thr Ile Val Gly Gly 630 635 640 645 gcc gat cca cgt aga gaa ggc gtg gtc ttg ggt ggc taattagcgc 2081 Ala Asp Pro Arg Arg Glu Gly Val Val Leu Gly Gly 650 655 gcagaccacg cagtccacag 2101 <210> 150 <211> 657 <212> PRT <213> Corynebacterium glutamicum <400> 150 Met Lys Val Thr Gln Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr 1 5 10 15 Val Ala Ala Leu Thr Leu Thr Ile Ser Ser Cys Ser Ser Gly Glu Asp 20 25 30 Thr Ser Ala Ser Ser Thr Asp Thr Glu Asn Ser Ser Thr Gln Ala Ala 35 40 45 Ala Ser Pro Pro Leu Ala Pro Cys Glu Leu Pro Ala Asp Ala Ser Ala 50 55 60 Glu Glu Glu Val Glu Gly Thr His Thr Gly Glu Asp Ile Ser Val Ala 65 70 75 80 Pro Glu Ile Gly Thr Gly Tyr Arg Glu Gly Met Thr Pro Val Gln Thr 85 90 95 Gln Gly Tyr Ala Val Ala Thr Ala Asn Pro Ile Ala Ser Glu Ala Ala 100 105 110 Cys Ala Val Leu Arg Glu Gly Gly Thr Ala Ala Asp Ala Leu Val Thr 115 120 125 Ala Gln Phe Val Leu Gly Leu Thr Glu Pro Gln Ser Ser Gly Leu Gly 130 135 140 Gly Gly Gly Tyr Ile Leu Tyr Tyr Asp Ala Glu Ala Asn Ala Val Thr 145 150 155 160 Ala Ile Asp Gly Arg Glu Thr Ala Pro Val Ala Ala Asp Glu Asn Tyr 165 170 175 Leu Ile His Val Ser Ala Glu Asp Gln Thr Ala Pro Val Pro Asp Ala 180 185 190 Arg Arg Ser Gly Arg Ser Ile Gly Val Pro Gly Ile Val Ala Ala Leu 195 200 205 Gly Gln Leu His Asp Ser Phe Gly Lys Thr Ser Trp Gln Asp Val Leu 210 215 220 Thr Thr Pro Gln Gln Leu Ala Thr Asp Gly Phe Ser Ile Ser Pro Arg 225 230 235 240 Met Ser Ala Ser Ile Ala Asn Ser Ala Glu Asp Leu Ser His Asp Pro 245 250 255 Glu Ala Ala Ala Tyr Phe Leu Asp Glu Asn Gly Asp Ala Lys Ala Pro 260 265 270 Gly Thr Leu Leu Gln Asn Pro Asp Tyr Ala Glu Thr Ile Arg Leu Ile 275 280 285 Ser Glu Gly Gly Pro Asp Ala Phe Tyr Thr Gly Glu Ile Ala Ala Asp 290 295 300 Ile Val Glu Arg Ala Thr Arg Glu Val Asp Gly Phe Thr Pro Ser Leu 305 310 315 320 Met Ser Thr Ala Asp Leu Ala Ala Tyr Thr Pro Glu Thr Arg Glu Ala 325 330 335 Leu Cys Ala Pro Tyr Arg Asp Lys Ile Val Cys Gly Met Pro Pro Ser 340 345 350 Ser Ser Gly Gly Val Thr Val Met Glu Thr Leu Gly Ile Leu Asn Asn 355 360 365 Phe Asp Leu Ala Gln Tyr Pro Pro Thr Glu Val Gly Leu Asp Gly Gly 370 375 380 Leu Pro Asn Ala Glu Ala Val His Leu Ile Ser Glu Ala Glu Arg Leu 385 390 395 400 Ala Tyr Ala Asp Arg Asp Ala Tyr Ile Gly Asp Pro Ala Phe Val Glu 405 410 415 Val Pro Ala Gly Gly Val Glu Glu Leu Ile Ser Asp Val Tyr Thr Gly 420 425 430 Glu Arg Ser Glu Leu Ile Asp Pro Glu His Ser Met Gly Gln Ala Thr 435 440 445 Ala Gly Leu Ser Gln Glu Pro Val Met Ala Ala Leu Pro Glu Ser Gly 450 455 460 Thr Ser His Ile Ser Ile Ile Asp Ser Tyr Gly Asn Ala Ala Ser Leu 465 470 475 480 Thr Thr Ser Val Glu Ala Ala Phe Gly Ser Phe His Phe Thr Arg Gly 485 490 495 Phe Ile Leu Asn Asn Gln Leu Thr Asp Phe Ser Ala Glu Pro Leu Asp 500 505 510 Glu Asp Gly Glu Pro Val Ala Asn Arg Val Glu Ser Ala Lys Arg Pro 515 520 525 Arg Ser Ser Met Ser Pro Met Leu Val Phe Asn Ala Ser Gly Asp Gly 530 535 540 Glu Ile Ala Asp Leu Asn Met Val Leu Gly Ser Pro Gly Gly Ser Leu 545 550 555 560 Ile Ile Gln Tyr Val Val Lys Thr Leu Val Asn Ile Ile Asp Trp Asp 565 570 575 Met Asp Pro Gln Gln Ala Val Ser Ala Pro Asn Phe Gly Ala Met Asn 580 585 590 Gln Pro Lys Thr Gly Leu Gly Ser Glu His Pro Leu Ile Ala Asn Asp 595 600 605 Ser Ala Glu Leu Val Ser Glu Leu Glu Ser Lys Gly His Glu Val Asn 610 615 620 Val Gly Glu Gln Ser Ser Gly Leu Ser Ala Leu Val Lys Asn Gly Asp 625 630 635 640 Thr Ile Val Gly Gly Ala Asp Pro Arg Arg Glu Gly Val Val Leu Gly 645 650 655 Gly <210> 151 <211> 886 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(856) <223> RXA04173 <400> 151 actgtccgaa gtattggatc tggtcgaggg aactgtccgt aaacgcgact aaacgacccc 60 tgattcacac tttcagacta caagaactag actaagcggt atg gtt tca gtt ctt 115 Met Val Ser Val Leu 1 5 tta att cag ccc cgt cag gga gaa gca gtc gcc gca gct gag cga cgt 163 Leu Ile Gln Pro Arg Gln Gly Glu Ala Val Ala Ala Ala Glu Arg Arg 10 15 20 gac ttt ttg cag gcc acc ggt ctt aag cct caa gaa ctg acc tcc cga 211 Asp Phe Leu Gln Ala Thr Gly Leu Lys Pro Gln Glu Leu Thr Ser Arg 25 30 35 atg ttg gat acc acc act tct cga att ggc agt ctg gaa ggt ttc gac 259 Met Leu Asp Thr Thr Thr Ser Arg Ile Gly Ser Leu Glu Gly Phe Asp 40 45 50 ggc gtg att gtg ggc gga agc cca ctg aat gcc acc aac ttt gag tac 307 Gly Val Ile Val Gly Gly Ser Pro Leu Asn Ala Thr Asn Phe Glu Tyr 55 60 65 agc gat tgg caa cgc cac gtc cac cgc gaa ttg tcc ttg ctg atc aat 355 Ser Asp Trp Gln Arg His Val His Arg Glu Leu Ser Leu Leu Ile Asn 70 75 80 85 cac cca ctg cca aca atc ttt gtc tgc tac ggc aat acc ttt ttg acc 403 His Pro Leu Pro Thr Ile Phe Val Cys Tyr Gly Asn Thr Phe Leu Thr 90 95 100 ttc ttc tct ggc gga cag att ggt cgc aca cac ccc gaa gat tcc ggc 451 Phe Phe Ser Gly Gly Gln Ile Gly Arg Thr His Pro Glu Asp Ser Gly 105 110 115 gcc acc aca gtg ttg cta act gac gcc ggc aaa cga gac gta ctc act 499 Ala Thr Thr Val Leu Leu Thr Asp Ala Gly Lys Arg Asp Val Leu Thr 120 125 130 caa gac cta ccg gat agc ttt acg tcc ttt act ggt cac acg gaa aac 547 Gln Asp Leu Pro Asp Ser Phe Thr Ser Phe Thr Gly His Thr Glu Asn 135 140 145 tcc gta gcg ccc gcc cct gga cac gtg gtg ttg gca acg gga cca acc 595 Ser Val Ala Pro Ala Pro Gly His Val Val Leu Ala Thr Gly Pro Thr 150 155 160 165 tgc ccc atc cag atg ctg cgc gcc aac aag aac acc tgg tca gtt caa 643 Cys Pro Ile Gln Met Leu Arg Ala Asn Lys Asn Thr Trp Ser Val Gln 170 175 180 ttc cat gcg gat atg gat gcc gta ggc atg aaa aac cgc atg gat ttt 691 Phe His Ala Asp Met Asp Ala Val Gly Met Lys Asn Arg Met Asp Phe 185 190 195 tac tcc aac tac ggc tac ttc tcc cca gaa gat tat gac cgc atc att 739 Tyr Ser Asn Tyr Gly Tyr Phe Ser Pro Glu Asp Tyr Asp Arg Ile Ile 200 205 210 gca gag cta ccc tct gtt gac tcc att tat gcc aac agg gtg ctc cgc 787 Ala Glu Leu Pro Ser Val Asp Ser Ile Tyr Ala Asn Arg Val Leu Arg 215 220 225 aac ttc gtg gag gtc tgc gaa gga att cgt gtt gct gat ggt gct gag 835 Asn Phe Val Glu Val Cys Glu Gly Ile Arg Val Ala Asp Gly Ala Glu 230 235 240 245 cac caa ctc cca aag ctt aac taatcgagga gactggtgat tcgccatcca 886 His Gln Leu Pro Lys Leu Asn 250 <210> 152 <211> 252 <212> PRT <213> Corynebacterium glutamicum <400> 152 Met Val Ser Val Leu Leu Ile Gln Pro Arg Gln Gly Glu Ala Val Ala 1 5 10 15 Ala Ala Glu Arg Arg Asp Phe Leu Gln Ala Thr Gly Leu Lys Pro Gln 20 25 30 Glu Leu Thr Ser Arg Met Leu Asp Thr Thr Thr Ser Arg Ile Gly Ser 35 40 45 Leu Glu Gly Phe Asp Gly Val Ile Val Gly Gly Ser Pro Leu Asn Ala 50 55 60 Thr Asn Phe Glu Tyr Ser Asp Trp Gln Arg His Val His Arg Glu Leu 65 70 75 80 Ser Leu Leu Ile Asn His Pro Leu Pro Thr Ile Phe Val Cys Tyr Gly 85 90 95 Asn Thr Phe Leu Thr Phe Phe Ser Gly Gly Gln Ile Gly Arg Thr His 100 105 110 Pro Glu Asp Ser Gly Ala Thr Thr Val Leu Leu Thr Asp Ala Gly Lys 115 120 125 Arg Asp Val Leu Thr Gln Asp Leu Pro Asp Ser Phe Thr Ser Phe Thr 130 135 140 Gly His Thr Glu Asn Ser Val Ala Pro Ala Pro Gly His Val Val Leu 145 150 155 160 Ala Thr Gly Pro Thr Cys Pro Ile Gln Met Leu Arg Ala Asn Lys Asn 165 170 175 Thr Trp Ser Val Gln Phe His Ala Asp Met Asp Ala Val Gly Met Lys 180 185 190 Asn Arg Met Asp Phe Tyr Ser Asn Tyr Gly Tyr Phe Ser Pro Glu Asp 195 200 205 Tyr Asp Arg Ile Ile Ala Glu Leu Pro Ser Val Asp Ser Ile Tyr Ala 210 215 220 Asn Arg Val Leu Arg Asn Phe Val Glu Val Cys Glu Gly Ile Arg Val 225 230 235 240 Ala Asp Gly Ala Glu His Gln Leu Pro Lys Leu Asn 245 250 <210> 153 <211> 2959 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(2929) <223> RXA04174 <400> 153 ccttttccac agttctgtgg aatgagaatc cgatgttttt ctcacgccgg ctcagccgaa 60 gcagacgccg tcgcgaaatc tcaccctaaa aaagttagaa ttg gag ctc act gtg 115 Leu Glu Leu Thr Val 1 5 act gaa agc aag aac tcc ttc aat gct aag agc acc ctt gaa gtt ggc 163 Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Ser Thr Leu Glu Val Gly 10 15 20 gac aag tcc tat gac tac ttc gcc ctc tct gca gtg cct ggc atg gag 211 Asp Lys Ser Tyr Asp Tyr Phe Ala Leu Ser Ala Val Pro Gly Met Glu 25 30 35 aag ctg ccg tac tcc ctc aag gtt ctc gga gag aac ctt ctt cgt acc 259 Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg Thr 40 45 50 gaa gac ggc gca aac atc acc aac gag cac att gag gct atc gcc aac 307 Glu Asp Gly Ala Asn Ile Thr Asn Glu His Ile Glu Ala Ile Ala Asn 55 60 65 tgg gat gca tct tcc gat cca agc atc gaa atc cag ttc acc cca gcc 355 Trp Asp Ala Ser Ser Asp Pro Ser Ile Glu Ile Gln Phe Thr Pro Ala 70 75 80 85 cgt gtt ctc atg cag gac ttc acc ggt gtc cct tgt gta gtt gac ctc 403 Arg Val Leu Met Gln Asp Phe Thr Gly Val Pro Cys Val Val Asp Leu 90 95 100 gca acc atg cgt gag gca gtt gct gca ctc ggt ggc gac cct aac gac 451 Ala Thr Met Arg Glu Ala Val Ala Ala Leu Gly Gly Asp Pro Asn Asp 105 110 115 gtc aac cca ctg aac cca gcc gag atg gtc att gac cac tcc gtc atc 499 Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val Ile 120 125 130 gtg gag gct ttc ggc cgc cca gat gca ctg gct aag aac gtt gag atc 547 Val Glu Ala Phe Gly Arg Pro Asp Ala Leu Ala Lys Asn Val Glu Ile 135 140 145 gag tac gag cgc aac gag gag cgt tac cag ttc ctg cgt tgg ggt tcc 595 Glu Tyr Glu Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly Ser 150 155 160 165 gag tcc ttc tcc aac ttc cgc gtt gtt cct cca gga acc ggt atc gtc 643 Glu Ser Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile Val 170 175 180 cac cag gtc aac att gag tac ttg gct cgc gtc gtc ttc gac aac gag 691 His Gln Val Asn Ile Glu Tyr Leu Ala Arg Val Val Phe Asp Asn Glu 185 190 195 ggc ctt gca tac cca gat acc tgc atc ggt acc gac tcc cac acc acc 739 Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr Thr 200 205 210 atg gaa aac ggc ctg ggc atc ctg ggc tgg ggc gtt ggt ggc att gag 787 Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile Glu 215 220 225 gct gaa gca gca atg ctc ggc cag cca gtg tcc atg ctg atc cct cgc 835 Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro Arg 230 235 240 245 gtt gtt ggc ttc aag ttg acc ggc gag atc cca gta ggc gtt acc gca 883 Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Val Gly Val Thr Ala 250 255 260 act gac gtt gtg ctg acc atc acc gaa atg ctg cgc gac cac ggc gtc 931 Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu Arg Asp His Gly Val 265 270 275 gtc cag aag ttc gtt gag ttc tac ggc tcc ggt gtt aag gct gtt cca 979 Val Gln Lys Phe Val Glu Phe Tyr Gly Ser Gly Val Lys Ala Val Pro 280 285 290 ctg gct aac cgt gca acc atc ggc aac atg tcc cca gag ttc ggc tcc 1027 Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser Pro Glu Phe Gly Ser 295 300 305 acc tgt gcg atg ttc cca atc gac gag gag acc acc aag tac ctg cgc 1075 Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr Thr Lys Tyr Leu Arg 310 315 320 325 ctc acc ggc cgc cca gaa gag cag gtt gca ctg gtc gag gct tac gcc 1123 Leu Thr Gly Arg Pro Glu Glu Gln Val Ala Leu Val Glu Ala Tyr Ala 330 335 340 aag gcg cag ggc atg tgg ctc gac gag gac acc gtt gaa gct gag tac 1171 Lys Ala Gln Gly Met Trp Leu Asp Glu Asp Thr Val Glu Ala Glu Tyr 345 350 355 tcc gag tac ctc gag ctg gac ctg tcc acc gtt gtt cct tcc atc gct 1219 Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val Val Pro Ser Ile Ala 360 365 370 ggc cct aag cgc cca cag gac cgc atc ctt ctc tcc gag gca aag gag 1267 Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu Ser Glu Ala Lys Glu 375 380 385 cag ttc cgt aag gat ctg cca acc tac acc gac gac gct gtt tcc gta 1315 Gln Phe Arg Lys Asp Leu Pro Thr Tyr Thr Asp Asp Ala Val Ser Val 390 395 400 405 gac acc tcc atc cct gca acc cgc atg gtt aac gaa ggt ggc gga cag 1363 Asp Thr Ser Ile Pro Ala Thr Arg Met Val Asn Glu Gly Gly Gly Gln 410 415 420 cct gaa ggc ggc gtc gaa gct gac aac tac aac gct tcc tgg gct ggc 1411 Pro Glu Gly Gly Val Glu Ala Asp Asn Tyr Asn Ala Ser Trp Ala Gly 425 430 435 tcc ggc gag tcc ttg gct act ggc gca gaa gga cgt cct tcc aag cca 1459 Ser Gly Glu Ser Leu Ala Thr Gly Ala Glu Gly Arg Pro Ser Lys Pro 440 445 450 gtc acc gtt gca tcc cca cag ggt ggc gag tac acc atc gac cac ggc 1507 Val Thr Val Ala Ser Pro Gln Gly Gly Glu Tyr Thr Ile Asp His Gly 455 460 465 atg gtt gca att gca tcc atc acc tct tgc acc aac acc tct aac cca 1555 Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr Asn Thr Ser Asn Pro 470 475 480 485 tcc gtg atg atc ggc gct ggc ctg atc gca cgt aag gca gca gaa aag 1603 Ser Val Met Ile Gly Ala Gly Leu Ile Ala Arg Lys Ala Ala Glu Lys 490 495 500 ggc ctc aag tcc aag cct tgg gtt aag acc atc tgt gca cca ggt tcc 1651 Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile Cys Ala Pro Gly Ser 505 510 515 cag gtt gtc gac ggc tac tac cag cgc gca gac ctc tgg aag gac ctt 1699 Gln Val Val Asp Gly Tyr Tyr Gln Arg Ala Asp Leu Trp Lys Asp Leu 520 525 530 gag gcc atg ggc ttc tac ctc tcc ggc ttc ggc tgc acc acc tgt att 1747 Glu Ala Met Gly Phe Tyr Leu Ser Gly Phe Gly Cys Thr Thr Cys Ile 535 540 545 ggt aac tcc ggc cca ctg cca gag gaa atc tcc gct gcg atc aac gag 1795 Gly Asn Ser Gly Pro Leu Pro Glu Glu Ile Ser Ala Ala Ile Asn Glu 550 555 560 565 cac gac ctg acc gca acc gca gtt ttg tcc ggt aac cgt aac ttc gag 1843 His Asp Leu Thr Ala Thr Ala Val Leu Ser Gly Asn Arg Asn Phe Glu 570 575 580 gga cgt atc tcc cct gac gtt aag atg aac tac ctg gca tcc cca atc 1891 Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr Leu Ala Ser Pro Ile 585 590 595 atg gtc att gct tac gca atc gct ggc acc atg gac ttc gac ttc gag 1939 Met Val Ile Ala Tyr Ala Ile Ala Gly Thr Met Asp Phe Asp Phe Glu 600 605 610 aac gaa gct ctt gga cag gac cag gac ggc aac gac gtc ttc ctg aag 1987 Asn Glu Ala Leu Gly Gln Asp Gln Asp Gly Asn Asp Val Phe Leu Lys 615 620 625 gac atc tgg cct tcc acc gag gaa atc gaa gac acc atc cag cag gca 2035 Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Asp Thr Ile Gln Gln Ala 630 635 640 645 atc tcc cgt gag ctt tac gaa gct gac tac gca gat gtc ttc aag ggt 2083 Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala Asp Val Phe Lys Gly 650 655 660 gac aag cag tgg cag gaa ctc gat gtt cct acc ggt gac acc ttc gag 2131 Asp Lys Gln Trp Gln Glu Leu Asp Val Pro Thr Gly Asp Thr Phe Glu 665 670 675 tgg gac gag aac tcc acc tac atc cgc aag gca cct tac ttc gac ggc 2179 Trp Asp Glu Asn Ser Thr Tyr Ile Arg Lys Ala Pro Tyr Phe Asp Gly 680 685 690 atg cct gtc gag cca gtg gca gtc acc gac atc cag ggc gca cgc gtt 2227 Met Pro Val Glu Pro Val Ala Val Thr Asp Ile Gln Gly Ala Arg Val 695 700 705 ctg gct aag ctc ggc gac tct gtc acc acc gac cac atc tcc cct gct 2275 Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp His Ile Ser Pro Ala 710 715 720 725 tcc tcc att aag cca ggt acc cct gca gct cag tac ttg gat gag cac 2323 Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln Tyr Leu Asp Glu His 730 735 740 ggt gtg gaa cgc cac gac tac aac tcc ctg ggt tcc agg cgt ggt aac 2371 Gly Val Glu Arg His Asp Tyr Asn Ser Leu Gly Ser Arg Arg Gly Asn 745 750 755 cac gag gtc atg atg cgc ggc acc ttc gcc aac atc cgc ctc cag aac 2419 His Glu Val Met Met Arg Gly Thr Phe Ala Asn Ile Arg Leu Gln Asn 760 765 770 cag ctg gtt gac atc gca ggt ggc tac acc cgc gac ttc acc cag gag 2467 Gln Leu Val Asp Ile Ala Gly Gly Tyr Thr Arg Asp Phe Thr Gln Glu 775 780 785 ggt gct cca cag gcg ttc atc tac gac gct tcc gtc aac tac aag gct 2515 Gly Ala Pro Gln Ala Phe Ile Tyr Asp Ala Ser Val Asn Tyr Lys Ala 790 795 800 805 gct ggc att ccg ctg gtc gtc ttg ggc ggc aag gag tac ggc acc ggt 2563 Ala Gly Ile Pro Leu Val Val Leu Gly Gly Lys Glu Tyr Gly Thr Gly 810 815 820 tct tcc cgt gac tgg gca gct aag ggc act aac ctg ctc gga att cgc 2611 Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Asn Leu Leu Gly Ile Arg 825 830 835 gca gtt atc acc gag tcc ttc gag cgt att cac cgc tcc aac ctc atc 2659 Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His Arg Ser Asn Leu Ile 840 845 850 ggt atg ggc gtt gtc cca ctg cag ttc cct gca ggc gaa tcc cac gag 2707 Gly Met Gly Val Val Pro Leu Gln Phe Pro Ala Gly Glu Ser His Glu 855 860 865 tcc ctg ggc ctt gac ggc acc gag acc ttc gac atc acc gga ctg acc 2755 Ser Leu Gly Leu Asp Gly Thr Glu Thr Phe Asp Ile Thr Gly Leu Thr 870 875 880 885 gca ctc aac gag ggc gag act cct aag act gtc aag gtc acc gca acc 2803 Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val Lys Val Thr Ala Thr 890 895 900 aag gag aac ggc gac gtc gtc gag ttc gac gca gtt gtc cgc atc gac 2851 Lys Glu Asn Gly Asp Val Val Glu Phe Asp Ala Val Val Arg Ile Asp 905 910 915 acc cca ggt gag gct gac tac tac cgc cac ggc ggc atc ctg cag tac 2899 Thr Pro Gly Glu Ala Asp Tyr Tyr Arg His Gly Gly Ile Leu Gln Tyr 920 925 930 gtg ctg cgt cag atg gct gct tct tct aag taattagaag acttttgatg 2949 Val Leu Arg Gln Met Ala Ala Ser Ser Lys 935 940 tggagtttcc 2959 <210> 154 <211> 943 <212> PRT <213> Corynebacterium glutamicum <400> 154 Leu Glu Leu Thr Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Ser 1 5 10 15 Thr Leu Glu Val Gly Asp Lys Ser Tyr Asp Tyr Phe Ala Leu Ser Ala 20 25 30 Val Pro Gly Met Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu 35 40 45 Asn Leu Leu Arg Thr Glu Asp Gly Ala Asn Ile Thr Asn Glu His Ile 50 55 60 Glu Ala Ile Ala Asn Trp Asp Ala Ser Ser Asp Pro Ser Ile Glu Ile 65 70 75 80 Gln Phe Thr Pro Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Pro 85 90 95 Cys Val Val Asp Leu Ala Thr Met Arg Glu Ala Val Ala Ala Leu Gly 100 105 110 Gly Asp Pro Asn Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile 115 120 125 Asp His Ser Val Ile Val Glu Ala Phe Gly Arg Pro Asp Ala Leu Ala 130 135 140 Lys Asn Val Glu Ile Glu Tyr Glu Arg Asn Glu Glu Arg Tyr Gln Phe 145 150 155 160 Leu Arg Trp Gly Ser Glu Ser Phe Ser Asn Phe Arg Val Val Pro Pro 165 170 175 Gly Thr Gly Ile Val His Gln Val Asn Ile Glu Tyr Leu Ala Arg Val 180 185 190 Val Phe Asp Asn Glu Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr 195 200 205 Asp Ser His Thr Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly 210 215 220 Val Gly Gly Ile Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser 225 230 235 240 Met Leu Ile Pro Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro 245 250 255 Val Gly Val Thr Ala Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu 260 265 270 Arg Asp His Gly Val Val Gln Lys Phe Val Glu Phe Tyr Gly Ser Gly 275 280 285 Val Lys Ala Val Pro Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser 290 295 300 Pro Glu Phe Gly Ser Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr 305 310 315 320 Thr Lys Tyr Leu Arg Leu Thr Gly Arg Pro Glu Glu Gln Val Ala Leu 325 330 335 Val Glu Ala Tyr Ala Lys Ala Gln Gly Met Trp Leu Asp Glu Asp Thr 340 345 350 Val Glu Ala Glu Tyr Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val 355 360 365 Val Pro Ser Ile Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu 370 375 380 Ser Glu Ala Lys Glu Gln Phe Arg Lys Asp Leu Pro Thr Tyr Thr Asp 385 390 395 400 Asp Ala Val Ser Val Asp Thr Ser Ile Pro Ala Thr Arg Met Val Asn 405 410 415 Glu Gly Gly Gly Gln Pro Glu Gly Gly Val Glu Ala Asp Asn Tyr Asn 420 425 430 Ala Ser Trp Ala Gly Ser Gly Glu Ser Leu Ala Thr Gly Ala Glu Gly 435 440 445 Arg Pro Ser Lys Pro Val Thr Val Ala Ser Pro Gln Gly Gly Glu Tyr 450 455 460 Thr Ile Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr 465 470 475 480 Asn Thr Ser Asn Pro Ser Val Met Ile Gly Ala Gly Leu Ile Ala Arg 485 490 495 Lys Ala Ala Glu Lys Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile 500 505 510 Cys Ala Pro Gly Ser Gln Val Val Asp Gly Tyr Tyr Gln Arg Ala Asp 515 520 525 Leu Trp Lys Asp Leu Glu Ala Met Gly Phe Tyr Leu Ser Gly Phe Gly 530 535 540 Cys Thr Thr Cys Ile Gly Asn Ser Gly Pro Leu Pro Glu Glu Ile Ser 545 550 555 560 Ala Ala Ile Asn Glu His Asp Leu Thr Ala Thr Ala Val Leu Ser Gly 565 570 575 Asn Arg Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr 580 585 590 Leu Ala Ser Pro Ile Met Val Ile Ala Tyr Ala Ile Ala Gly Thr Met 595 600 605 Asp Phe Asp Phe Glu Asn Glu Ala Leu Gly Gln Asp Gln Asp Gly Asn 610 615 620 Asp Val Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Asp 625 630 635 640 Thr Ile Gln Gln Ala Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala 645 650 655 Asp Val Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Val Pro Thr 660 665 670 Gly Asp Thr Phe Glu Trp Asp Glu Asn Ser Thr Tyr Ile Arg Lys Ala 675 680 685 Pro Tyr Phe Asp Gly Met Pro Val Glu Pro Val Ala Val Thr Asp Ile 690 695 700 Gln Gly Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp 705 710 715 720 His Ile Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln 725 730 735 Tyr Leu Asp Glu His Gly Val Glu Arg His Asp Tyr Asn Ser Leu Gly 740 745 750 Ser Arg Arg Gly Asn His Glu Val Met Met Arg Gly Thr Phe Ala Asn 755 760 765 Ile Arg Leu Gln Asn Gln Leu Val Asp Ile Ala Gly Gly Tyr Thr Arg 770 775 780 Asp Phe Thr Gln Glu Gly Ala Pro Gln Ala Phe Ile Tyr Asp Ala Ser 785 790 795 800 Val Asn Tyr Lys Ala Ala Gly Ile Pro Leu Val Val Leu Gly Gly Lys 805 810 815 Glu Tyr Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Asn 820 825 830 Leu Leu Gly Ile Arg Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His 835 840 845 Arg Ser Asn Leu Ile Gly Met Gly Val Val Pro Leu Gln Phe Pro Ala 850 855 860 Gly Glu Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Thr Phe Asp 865 870 875 880 Ile Thr Gly Leu Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val 885 890 895 Lys Val Thr Ala Thr Lys Glu Asn Gly Asp Val Val Glu Phe Asp Ala 900 905 910 Val Val Arg Ile Asp Thr Pro Gly Glu Ala Asp Tyr Tyr Arg His Gly 915 920 925 Gly Ile Leu Gln Tyr Val Leu Arg Gln Met Ala Ala Ser Ser Lys 930 935 940 <210> 155 <211> 3793 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(3763) <223> RXA04186 <400> 155 agactagtgg cgctttgcct gtgttgctta ggcggcgttg aaaatgaact acgaatgaaa 60 agttcgggaa ttgtctaatc cgtactaagc tgtctacaca atg tct act tca gtt 115 Met Ser Thr Ser Val 1 5 act tca cca gcc cac aac aac gca cat tcc tcc gaa ttt ttg gat gcg 163 Thr Ser Pro Ala His Asn Asn Ala His Ser Ser Glu Phe Leu Asp Ala 10 15 20 ttg gca aac cat gtg ttg atc ggc gac ggc gcc atg ggc acc cag ctc 211 Leu Ala Asn His Val Leu Ile Gly Asp Gly Ala Met Gly Thr Gln Leu 25 30 35 caa ggc ttt gac ctg gac gtg gaa aag gat ttc ctt gat ctg gag ggg 259 Gln Gly Phe Asp Leu Asp Val Glu Lys Asp Phe Leu Asp Leu Glu Gly 40 45 50 tgt aat gag att ctc aac gac acc cgc cct gat gtg ttg agg cag att 307 Cys Asn Glu Ile Leu Asn Asp Thr Arg Pro Asp Val Leu Arg Gln Ile 55 60 65 cac cgc gcc tac ttt gag gcg gga gct gac ttg gtt gag acc aat act 355 His Arg Ala Tyr Phe Glu Ala Gly Ala Asp Leu Val Glu Thr Asn Thr 70 75 80 85 ttt ggt tgc aac ctg ccg aac ttg gcg gat tat gac atc gct gat cgt 403 Phe Gly Cys Asn Leu Pro Asn Leu Ala Asp Tyr Asp Ile Ala Asp Arg 90 95 100 tgc cgt gag ctt gcc tac aag ggc act gca gtg gct agg gaa gtg gct 451 Cys Arg Glu Leu Ala Tyr Lys Gly Thr Ala Val Ala Arg Glu Val Ala 105 110 115 gat gag atg ggg ccg ggc cga aac ggc atg cgg cgt ttc gtg gtt ggt 499 Asp Glu Met Gly Pro Gly Arg Asn Gly Met Arg Arg Phe Val Val Gly 120 125 130 tcc ctg gga cct gga acg aag ctt cca tcg ctg ggc cat gca ccg tat 547 Ser Leu Gly Pro Gly Thr Lys Leu Pro Ser Leu Gly His Ala Pro Tyr 135 140 145 gca gat ttg cgt ggg cac tac aag gaa gca gcg ctt ggc atc atc gac 595 Ala Asp Leu Arg Gly His Tyr Lys Glu Ala Ala Leu Gly Ile Ile Asp 150 155 160 165 ggt ggt ggc gat gcc ttt ttg att gag act gct cag gac ttg ctt cag 643 Gly Gly Gly Asp Ala Phe Leu Ile Glu Thr Ala Gln Asp Leu Leu Gln 170 175 180 gtc aag gct gcg gtt cac ggc gtt caa gat gcc atg gct gaa ctt gat 691 Val Lys Ala Ala Val His Gly Val Gln Asp Ala Met Ala Glu Leu Asp 185 190 195 aca ttc ttg ccc att att tgc cac gtc acc gta gag acc acc ggc acc 739 Thr Phe Leu Pro Ile Ile Cys His Val Thr Val Glu Thr Thr Gly Thr 200 205 210 atg ctc atg ggt tct gag atc ggt gcc gcg ttg aca gcg ctg cag cca 787 Met Leu Met Gly Ser Glu Ile Gly Ala Ala Leu Thr Ala Leu Gln Pro 215 220 225 ctg ggt atc gac atg att ggt ctg aac tgc gcc acc ggc cca gat gag 835 Leu Gly Ile Asp Met Ile Gly Leu Asn Cys Ala Thr Gly Pro Asp Glu 230 235 240 245 atg agc gag cac ctg cgt tac ctg tcc aag cac gcc gat att cct gtg 883 Met Ser Glu His Leu Arg Tyr Leu Ser Lys His Ala Asp Ile Pro Val 250 255 260 tcg gtg atg cct aac gca ggt ctt cct gtc ctg ggt aaa aac ggt gca 931 Ser Val Met Pro Asn Ala Gly Leu Pro Val Leu Gly Lys Asn Gly Ala 265 270 275 gaa tac cca ctt gag gct gag gat ttg gcg cag gcg ctg gct gga ttc 979 Glu Tyr Pro Leu Glu Ala Glu Asp Leu Ala Gln Ala Leu Ala Gly Phe 280 285 290 gtc tcc gaa tat ggc ctg tcc atg gtg ggt ggt tgt tgt ggc acc aca 1027 Val Ser Glu Tyr Gly Leu Ser Met Val Gly Gly Cys Cys Gly Thr Thr 295 300 305 cct gag cac atc cgt gcg gtc cgc gat gcg gtg gtt ggt gtt cca gag 1075 Pro Glu His Ile Arg Ala Val Arg Asp Ala Val Val Gly Val Pro Glu 310 315 320 325 cag gaa acc tcc aca ctg acc aag atc cct gca ggc cct gtt gag cag 1123 Gln Glu Thr Ser Thr Leu Thr Lys Ile Pro Ala Gly Pro Val Glu Gln 330 335 340 gcc tcc cgc gag gtg gag aaa gag gac tcc gtc gcg tcg ctg tac acc 1171 Ala Ser Arg Glu Val Glu Lys Glu Asp Ser Val Ala Ser Leu Tyr Thr 345 350 355 tcg gtg cca ttg tcc cag gaa acc ggc att tcc atg atc ggt gag cgc 1219 Ser Val Pro Leu Ser Gln Glu Thr Gly Ile Ser Met Ile Gly Glu Arg 360 365 370 acc aac tcc aac ggt tcc aag gca ttc cgt gag gca atg ctg tct ggc 1267 Thr Asn Ser Asn Gly Ser Lys Ala Phe Arg Glu Ala Met Leu Ser Gly 375 380 385 gat tgg gaa aag tgt gtg gat att gcc aag cag caa acc cgc gat ggt 1315 Asp Trp Glu Lys Cys Val Asp Ile Ala Lys Gln Gln Thr Arg Asp Gly 390 395 400 405 gca cac atg ctg gat ctt tgt gtg gat tac gtg gga cga gac ggc acc 1363 Ala His Met Leu Asp Leu Cys Val Asp Tyr Val Gly Arg Asp Gly Thr 410 415 420 gcc gat atg gcg acc ttg gca gca ctt ctt gct acc agc tcc act ttg 1411 Ala Asp Met Ala Thr Leu Ala Ala Leu Leu Ala Thr Ser Ser Thr Leu 425 430 435 cca atc atg att gac tcc acc gag cca gag gtt att cgc aca ggc ctt 1459 Pro Ile Met Ile Asp Ser Thr Glu Pro Glu Val Ile Arg Thr Gly Leu 440 445 450 gag cac ttg ggt gga cga agc atc gtt aac tcc gtc aac ttt gaa gac 1507 Glu His Leu Gly Gly Arg Ser Ile Val Asn Ser Val Asn Phe Glu Asp 455 460 465 ggc gat ggc cct gag tcc cgc tac cag cgc atc atg aaa ctg gta aag 1555 Gly Asp Gly Pro Glu Ser Arg Tyr Gln Arg Ile Met Lys Leu Val Lys 470 475 480 485 cag cac ggt gcg gcc gtg gtt gcg ctg acc att gat gag gaa ggc cag 1603 Gln His Gly Ala Ala Val Val Ala Leu Thr Ile Asp Glu Glu Gly Gln 490 495 500 gca cgt acc gct gag cac aag gtg cgc att gct aaa cga ctg att gac 1651 Ala Arg Thr Ala Glu His Lys Val Arg Ile Ala Lys Arg Leu Ile Asp 505 510 515 gat atc acc ggc agc tac ggc ctg gat atc aaa gac atc gtt gtg gac 1699 Asp Ile Thr Gly Ser Tyr Gly Leu Asp Ile Lys Asp Ile Val Val Asp 520 525 530 tgc ctg acc ttc ccg atc tct act ggc cag gaa gaa acc agg cga gat 1747 Cys Leu Thr Phe Pro Ile Ser Thr Gly Gln Glu Glu Thr Arg Arg Asp 535 540 545 ggc att gaa acc atc gaa gcc atc cgc gag ctg aag aag ctc tac cca 1795 Gly Ile Glu Thr Ile Glu Ala Ile Arg Glu Leu Lys Lys Leu Tyr Pro 550 555 560 565 gaa atc cac acc acc ctg ggt ctg tcc aat att tcc ttc ggc ctg aac 1843 Glu Ile His Thr Thr Leu Gly Leu Ser Asn Ile Ser Phe Gly Leu Asn 570 575 580 cct gct gca cgc cag gtt ctt aac tct gtg ttc ctc aat gag tgc att 1891 Pro Ala Ala Arg Gln Val Leu Asn Ser Val Phe Leu Asn Glu Cys Ile 585 590 595 gag gct ggt ctg gac tct gcg att gcg cac agc tcc aag att ttg ccg 1939 Glu Ala Gly Leu Asp Ser Ala Ile Ala His Ser Ser Lys Ile Leu Pro 600 605 610 atg aac cgc att gat gat cgc cag cgc gaa gtg gcg ttg gat atg gtc 1987 Met Asn Arg Ile Asp Asp Arg Gln Arg Glu Val Ala Leu Asp Met Val 615 620 625 tat gat cgc cgc acc gag gat tac gat ccg ctg cag gaa ttc atg cag 2035 Tyr Asp Arg Arg Thr Glu Asp Tyr Asp Pro Leu Gln Glu Phe Met Gln 630 635 640 645 ctg ttt gag ggc gtt tct gct gcc gat gcc aag gat gct cgc gct gaa 2083 Leu Phe Glu Gly Val Ser Ala Ala Asp Ala Lys Asp Ala Arg Ala Glu 650 655 660 cag ctg gcc gct atg cct ttg ttt gag cgt ttg gca cag cgc atc atc 2131 Gln Leu Ala Ala Met Pro Leu Phe Glu Arg Leu Ala Gln Arg Ile Ile 665 670 675 gac ggc gat aag aat ggc ctt gag gat gat ctg gaa gca ggc atg aag 2179 Asp Gly Asp Lys Asn Gly Leu Glu Asp Asp Leu Glu Ala Gly Met Lys 680 685 690 gag aag tct cct att gcg atc atc aac gag gac ctt ctc aac ggc atg 2227 Glu Lys Ser Pro Ile Ala Ile Ile Asn Glu Asp Leu Leu Asn Gly Met 695 700 705 aag acc gtg ggt gag ctg ttt ggt tcc gga cag atg cag ctg cca ttc 2275 Lys Thr Val Gly Glu Leu Phe Gly Ser Gly Gln Met Gln Leu Pro Phe 710 715 720 725 gtg ctg caa tcg gca gaa acc atg aaa act gcg gtg gcc tat ttg gaa 2323 Val Leu Gln Ser Ala Glu Thr Met Lys Thr Ala Val Ala Tyr Leu Glu 730 735 740 ccg ttc atg gaa gag gaa gca gaa gct acc gga tct gcg cag gca gag 2371 Pro Phe Met Glu Glu Glu Ala Glu Ala Thr Gly Ser Ala Gln Ala Glu 745 750 755 ggc aag ggc aaa atc gtc gtg gcc acc gtc aag ggt gac gtg cac gat 2419 Gly Lys Gly Lys Ile Val Val Ala Thr Val Lys Gly Asp Val His Asp 760 765 770 atc ggc aag aac ttg gtg gac atc att ttg tcc aac aac ggt tac gac 2467 Ile Gly Lys Asn Leu Val Asp Ile Ile Leu Ser Asn Asn Gly Tyr Asp 775 780 785 gtg gtg aac ttg ggc atc aag cag cca ctg tcc gcc atg ttg gaa gca 2515 Val Val Asn Leu Gly Ile Lys Gln Pro Leu Ser Ala Met Leu Glu Ala 790 795 800 805 gcg gaa gaa cac aaa gca gac gtc atc ggc atg tcg gga ctt ctt gtg 2563 Ala Glu Glu His Lys Ala Asp Val Ile Gly Met Ser Gly Leu Leu Val 810 815 820 aag tcc acc gtg gtg atg aag gaa aac ctt gag gag atg aac aac gcc 2611 Lys Ser Thr Val Val Met Lys Glu Asn Leu Glu Glu Met Asn Asn Ala 825 830 835 ggc gca tcc aat tac cca gtc att ttg ggt ggc gct gcg ctg acg cgt 2659 Gly Ala Ser Asn Tyr Pro Val Ile Leu Gly Gly Ala Ala Leu Thr Arg 840 845 850 acc tac gtg gaa aac gat ctc aac gag gtg tac acc ggt gag gtg tac 2707 Thr Tyr Val Glu Asn Asp Leu Asn Glu Val Tyr Thr Gly Glu Val Tyr 855 860 865 tac gcc cgt gat gct ttc gag ggc ctg cgc ctg atg gat gag gtg atg 2755 Tyr Ala Arg Asp Ala Phe Glu Gly Leu Arg Leu Met Asp Glu Val Met 870 875 880 885 gca gaa aag cgt ggt gaa gga ctt gat ccc aac tca cca gaa gct att 2803 Ala Glu Lys Arg Gly Glu Gly Leu Asp Pro Asn Ser Pro Glu Ala Ile 890 895 900 gag cag gcg aag aag aag gcg gaa cgt aag gct cgt aat gag cgt tcc 2851 Glu Gln Ala Lys Lys Lys Ala Glu Arg Lys Ala Arg Asn Glu Arg Ser 905 910 915 cgc aag att gcc gcg gag cgt aaa gct aat gcg gct ccc gtg att gtt 2899 Arg Lys Ile Ala Ala Glu Arg Lys Ala Asn Ala Ala Pro Val Ile Val 920 925 930 ccg gag cgt tct gat gtc tcc acc gat act cca acc gcg gca cca ccg 2947 Pro Glu Arg Ser Asp Val Ser Thr Asp Thr Pro Thr Ala Ala Pro Pro 935 940 945 ttc tgg gga acc cgc att gtc aag ggt ctg ccc ttg gcg gag ttc ttg 2995 Phe Trp Gly Thr Arg Ile Val Lys Gly Leu Pro Leu Ala Glu Phe Leu 950 955 960 965 ggc aac ctt gat gag cgc gcc ttg ttc atg ggg cag tgg ggt ctg aaa 3043 Gly Asn Leu Asp Glu Arg Ala Leu Phe Met Gly Gln Trp Gly Leu Lys 970 975 980 tcc acc cgc ggc aac gag ggt cca agc tat gag gat ttg gtg gaa act 3091 Ser Thr Arg Gly Asn Glu Gly Pro Ser Tyr Glu Asp Leu Val Glu Thr 985 990 995 gaa ggc cga cca cgc ctg cgc tac tgg ctg gat cgc ctg aag tct gag 3139 Glu Gly Arg Pro Arg Leu Arg Tyr Trp Leu Asp Arg Leu Lys Ser Glu 1000 1005 1010 ggc att ttg gac cac gtg gcc ttg gtg tat ggc tac ttc cca gcg gtc 3187 Gly Ile Leu Asp His Val Ala Leu Val Tyr Gly Tyr Phe Pro Ala Val 1015 1020 1025 gcg gaa ggc gat gac gtg gtg atc ttg gaa tcc ccg gat cca cac gca 3235 Ala Glu Gly Asp Asp Val Val Ile Leu Glu Ser Pro Asp Pro His Ala 1030 1035 1040 1045 gcc gaa cgc atg cgc ttt agc ttc cca cgc cag cag cgc ggc agg ttc 3283 Ala Glu Arg Met Arg Phe Ser Phe Pro Arg Gln Gln Arg Gly Arg Phe 1050 1055 1060 ttg tgc atc gcg gat ttc att cgc cca cgc gag caa gct gtc aag gac 3331 Leu Cys Ile Ala Asp Phe Ile Arg Pro Arg Glu Gln Ala Val Lys Asp 1065 1070 1075 ggc caa gtg gac gtc atg cca ttc cag ctg gtc acc atg ggt aat cct 3379 Gly Gln Val Asp Val Met Pro Phe Gln Leu Val Thr Met Gly Asn Pro 1080 1085 1090 att gct gat ttc gcc aac gag ttg ttc gca gcc aat gaa tac cgc gag 3427 Ile Ala Asp Phe Ala Asn Glu Leu Phe Ala Ala Asn Glu Tyr Arg Glu 1095 1100 1105 tac ttg gaa gtt cac ggc atc ggc gtg cag ctc acc gaa gca ttg gcc 3475 Tyr Leu Glu Val His Gly Ile Gly Val Gln Leu Thr Glu Ala Leu Ala 1110 1115 1120 1125 gag tac tgg cac tcc cga gtg cgc agc gaa ctc aag ctg aac gac ggt 3523 Glu Tyr Trp His Ser Arg Val Arg Ser Glu Leu Lys Leu Asn Asp Gly 1130 1135 1140 gga tct gtc gct gat ttt gat cca gaa gac aag acc aag ttc ttc gac 3571 Gly Ser Val Ala Asp Phe Asp Pro Glu Asp Lys Thr Lys Phe Phe Asp 1145 1150 1155 ctg gat tac cgc ggc gcc cgc ttc tcc ttt ggt tac ggt tct tgc cct 3619 Leu Asp Tyr Arg Gly Ala Arg Phe Ser Phe Gly Tyr Gly Ser Cys Pro 1160 1165 1170 gat ctg gaa gac cgc gca aag ctg gtg gaa ttg ctc gag cca ggc cgt 3667 Asp Leu Glu Asp Arg Ala Lys Leu Val Glu Leu Leu Glu Pro Gly Arg 1175 1180 1185 atc ggc gtg gag ttg tcc gag gaa ctc cag ctg cac cca gag cag tcc 3715 Ile Gly Val Glu Leu Ser Glu Glu Leu Gln Leu His Pro Glu Gln Ser 1190 1195 1200 1205 aca gac gcg ttt gtg ctc tac cac cca gag gca aag tac ttt aac gtc 3763 Thr Asp Ala Phe Val Leu Tyr His Pro Glu Ala Lys Tyr Phe Asn Val 1210 1215 1220 taacaccttt gagagggaaa actttcccgc 3793 <210> 156 <211> 1221 <212> PRT <213> Corynebacterium glutamicum <400> 156 Met Ser Thr Ser Val Thr Ser Pro Ala His Asn Asn Ala His Ser Ser 1 5 10 15 Glu Phe Leu Asp Ala Leu Ala Asn His Val Leu Ile Gly Asp Gly Ala 20 25 30 Met Gly Thr Gln Leu Gln Gly Phe Asp Leu Asp Val Glu Lys Asp Phe 35 40 45 Leu Asp Leu Glu Gly Cys Asn Glu Ile Leu Asn Asp Thr Arg Pro Asp 50 55 60 Val Leu Arg Gln Ile His Arg Ala Tyr Phe Glu Ala Gly Ala Asp Leu 65 70 75 80 Val Glu Thr Asn Thr Phe Gly Cys Asn Leu Pro Asn Leu Ala Asp Tyr 85 90 95 Asp Ile Ala Asp Arg Cys Arg Glu Leu Ala Tyr Lys Gly Thr Ala Val 100 105 110 Ala Arg Glu Val Ala Asp Glu Met Gly Pro Gly Arg Asn Gly Met Arg 115 120 125 Arg Phe Val Val Gly Ser Leu Gly Pro Gly Thr Lys Leu Pro Ser Leu 130 135 140 Gly His Ala Pro Tyr Ala Asp Leu Arg Gly His Tyr Lys Glu Ala Ala 145 150 155 160 Leu Gly Ile Ile Asp Gly Gly Gly Asp Ala Phe Leu Ile Glu Thr Ala 165 170 175 Gln Asp Leu Leu Gln Val Lys Ala Ala Val His Gly Val Gln Asp Ala 180 185 190 Met Ala Glu Leu Asp Thr Phe Leu Pro Ile Ile Cys His Val Thr Val 195 200 205 Glu Thr Thr Gly Thr Met Leu Met Gly Ser Glu Ile Gly Ala Ala Leu 210 215 220 Thr Ala Leu Gln Pro Leu Gly Ile Asp Met Ile Gly Leu Asn Cys Ala 225 230 235 240 Thr Gly Pro Asp Glu Met Ser Glu His Leu Arg Tyr Leu Ser Lys His 245 250 255 Ala Asp Ile Pro Val Ser Val Met Pro Asn Ala Gly Leu Pro Val Leu 260 265 270 Gly Lys Asn Gly Ala Glu Tyr Pro Leu Glu Ala Glu Asp Leu Ala Gln 275 280 285 Ala Leu Ala Gly Phe Val Ser Glu Tyr Gly Leu Ser Met Val Gly Gly 290 295 300 Cys Cys Gly Thr Thr Pro Glu His Ile Arg Ala Val Arg Asp Ala Val 305 310 315 320 Val Gly Val Pro Glu Gln Glu Thr Ser Thr Leu Thr Lys Ile Pro Ala 325 330 335 Gly Pro Val Glu Gln Ala Ser Arg Glu Val Glu Lys Glu Asp Ser Val 340 345 350 Ala Ser Leu Tyr Thr Ser Val Pro Leu Ser Gln Glu Thr Gly Ile Ser 355 360 365 Met Ile Gly Glu Arg Thr Asn Ser Asn Gly Ser Lys Ala Phe Arg Glu 370 375 380 Ala Met Leu Ser Gly Asp Trp Glu Lys Cys Val Asp Ile Ala Lys Gln 385 390 395 400 Gln Thr Arg Asp Gly Ala His Met Leu Asp Leu Cys Val Asp Tyr Val 405 410 415 Gly Arg Asp Gly Thr Ala Asp Met Ala Thr Leu Ala Ala Leu Leu Ala 420 425 430 Thr Ser Ser Thr Leu Pro Ile Met Ile Asp Ser Thr Glu Pro Glu Val 435 440 445 Ile Arg Thr Gly Leu Glu His Leu Gly Gly Arg Ser Ile Val Asn Ser 450 455 460 Val Asn Phe Glu Asp Gly Asp Gly Pro Glu Ser Arg Tyr Gln Arg Ile 465 470 475 480 Met Lys Leu Val Lys Gln His Gly Ala Ala Val Val Ala Leu Thr Ile 485 490 495 Asp Glu Glu Gly Gln Ala Arg Thr Ala Glu His Lys Val Arg Ile Ala 500 505 510 Lys Arg Leu Ile Asp Asp Ile Thr Gly Ser Tyr Gly Leu Asp Ile Lys 515 520 525 Asp Ile Val Val Asp Cys Leu Thr Phe Pro Ile Ser Thr Gly Gln Glu 530 535 540 Glu Thr Arg Arg Asp Gly Ile Glu Thr Ile Glu Ala Ile Arg Glu Leu 545 550 555 560 Lys Lys Leu Tyr Pro Glu Ile His Thr Thr Leu Gly Leu Ser Asn Ile 565 570 575 Ser Phe Gly Leu Asn Pro Ala Ala Arg Gln Val Leu Asn Ser Val Phe 580 585 590 Leu Asn Glu Cys Ile Glu Ala Gly Leu Asp Ser Ala Ile Ala His Ser 595 600 605 Ser Lys Ile Leu Pro Met Asn Arg Ile Asp Asp Arg Gln Arg Glu Val 610 615 620 Ala Leu Asp Met Val Tyr Asp Arg Arg Thr Glu Asp Tyr Asp Pro Leu 625 630 635 640 Gln Glu Phe Met Gln Leu Phe Glu Gly Val Ser Ala Ala Asp Ala Lys 645 650 655 Asp Ala Arg Ala Glu Gln Leu Ala Ala Met Pro Leu Phe Glu Arg Leu 660 665 670 Ala Gln Arg Ile Ile Asp Gly Asp Lys Asn Gly Leu Glu Asp Asp Leu 675 680 685 Glu Ala Gly Met Lys Glu Lys Ser Pro Ile Ala Ile Ile Asn Glu Asp 690 695 700 Leu Leu Asn Gly Met Lys Thr Val Gly Glu Leu Phe Gly Ser Gly Gln 705 710 715 720 Met Gln Leu Pro Phe Val Leu Gln Ser Ala Glu Thr Met Lys Thr Ala 725 730 735 Val Ala Tyr Leu Glu Pro Phe Met Glu Glu Glu Ala Glu Ala Thr Gly 740 745 750 Ser Ala Gln Ala Glu Gly Lys Gly Lys Ile Val Val Ala Thr Val Lys 755 760 765 Gly Asp Val His Asp Ile Gly Lys Asn Leu Val Asp Ile Ile Leu Ser 770 775 780 Asn Asn Gly Tyr Asp Val Val Asn Leu Gly Ile Lys Gln Pro Leu Ser 785 790 795 800 Ala Met Leu Glu Ala Ala Glu Glu His Lys Ala Asp Val Ile Gly Met 805 810 815 Ser Gly Leu Leu Val Lys Ser Thr Val Val Met Lys Glu Asn Leu Glu 820 825 830 Glu Met Asn Asn Ala Gly Ala Ser Asn Tyr Pro Val Ile Leu Gly Gly 835 840 845 Ala Ala Leu Thr Arg Thr Tyr Val Glu Asn Asp Leu Asn Glu Val Tyr 850 855 860 Thr Gly Glu Val Tyr Tyr Ala Arg Asp Ala Phe Glu Gly Leu Arg Leu 865 870 875 880 Met Asp Glu Val Met Ala Glu Lys Arg Gly Glu Gly Leu Asp Pro Asn 885 890 895 Ser Pro Glu Ala Ile Glu Gln Ala Lys Lys Lys Ala Glu Arg Lys Ala 900 905 910 Arg Asn Glu Arg Ser Arg Lys Ile Ala Ala Glu Arg Lys Ala Asn Ala 915 920 925 Ala Pro Val Ile Val Pro Glu Arg Ser Asp Val Ser Thr Asp Thr Pro 930 935 940 Thr Ala Ala Pro Pro Phe Trp Gly Thr Arg Ile Val Lys Gly Leu Pro 945 950 955 960 Leu Ala Glu Phe Leu Gly Asn Leu Asp Glu Arg Ala Leu Phe Met Gly 965 970 975 Gln Trp Gly Leu Lys Ser Thr Arg Gly Asn Glu Gly Pro Ser Tyr Glu 980 985 990 Asp Leu Val Glu Thr Glu Gly Arg Pro Arg Leu Arg Tyr Trp Leu Asp 995 1000 1005 Arg Leu Lys Ser Glu Gly Ile Leu Asp His Val Ala Leu Val Tyr Gly 1010 1015 1020 Tyr Phe Pro Ala Val Ala Glu Gly Asp Asp Val Val Ile Leu Glu Ser 1025 1030 1035 1040 Pro Asp Pro His Ala Ala Glu Arg Met Arg Phe Ser Phe Pro Arg Gln 1045 1050 1055 Gln Arg Gly Arg Phe Leu Cys Ile Ala Asp Phe Ile Arg Pro Arg Glu 1060 1065 1070 Gln Ala Val Lys Asp Gly Gln Val Asp Val Met Pro Phe Gln Leu Val 1075 1080 1085 Thr Met Gly Asn Pro Ile Ala Asp Phe Ala Asn Glu Leu Phe Ala Ala 1090 1095 1100 Asn Glu Tyr Arg Glu Tyr Leu Glu Val His Gly Ile Gly Val Gln Leu 1105 1110 1115 1120 Thr Glu Ala Leu Ala Glu Tyr Trp His Ser Arg Val Arg Ser Glu Leu 1125 1130 1135 Lys Leu Asn Asp Gly Gly Ser Val Ala Asp Phe Asp Pro Glu Asp Lys 1140 1145 1150 Thr Lys Phe Phe Asp Leu Asp Tyr Arg Gly Ala Arg Phe Ser Phe Gly 1155 1160 1165 Tyr Gly Ser Cys Pro Asp Leu Glu Asp Arg Ala Lys Leu Val Glu Leu 1170 1175 1180 Leu Glu Pro Gly Arg Ile Gly Val Glu Leu Ser Glu Glu Leu Gln Leu 1185 1190 1195 1200 His Pro Glu Gln Ser Thr Asp Ala Phe Val Leu Tyr His Pro Glu Ala 1205 1210 1215 Lys Tyr Phe Asn Val 1220 <210> 157 <211> 1468 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1438) <223> RXA04228 <400> 157 tttccacacg cgttgcggat tttctccgca gtggaaaaac tcactcgccc aggctgcgaa 60 aacgcccgcg acacagtgga aggggagacg ccagcgactt ttg cga cat cat aaa 115 Leu Arg His His Lys 1 5 tgg tgg ctt ttg agt cgc tgt gag ccc cag aat ctg tca tgc aca aga 163 Trp Trp Leu Leu Ser Arg Cys Glu Pro Gln Asn Leu Ser Cys Thr Arg 10 15 20 gta tat agc gca aaa gaa atc act agt ctt gat tct atg ttg acg atg 211 Val Tyr Ser Ala Lys Glu Ile Thr Ser Leu Asp Ser Met Leu Thr Met 25 30 35 ccg ata ccc gag tac ctg cac gaa att tta gat gat gtc cgc gac acc 259 Pro Ile Pro Glu Tyr Leu His Glu Ile Leu Asp Asp Val Arg Asp Thr 40 45 50 acc tcc ggc gag ttg gcc gat tac atc ccg gaa cta aaa tct gcg gac 307 Thr Ser Gly Glu Leu Ala Asp Tyr Ile Pro Glu Leu Lys Ser Ala Asp 55 60 65 cca aac ccg ctg gca gta gcc ctg tgc acc gtt aac gga cac atc tac 355 Pro Asn Pro Leu Ala Val Ala Leu Cys Thr Val Asn Gly His Ile Tyr 70 75 80 85 agc gca ggc gat gac gac atc gaa ttc acc atg caa agt att tcc aag 403 Ser Ala Gly Asp Asp Asp Ile Glu Phe Thr Met Gln Ser Ile Ser Lys 90 95 100 cca ttt gcc tac gca ctc gca ctc caa gaa tgc ggc ttt gat gag gtc 451 Pro Phe Ala Tyr Ala Leu Ala Leu Gln Glu Cys Gly Phe Asp Glu Val 105 110 115 tct gca tcc gtg gcc ttg gag ccc tcc ggt gag gcc ttc aac gaa ctt 499 Ser Ala Ser Val Ala Leu Glu Pro Ser Gly Glu Ala Phe Asn Glu Leu 120 125 130 tcc ctc gac ggc gaa aac cgc ccc atg aac ccc atg atc aac gcc ggc 547 Ser Leu Asp Gly Glu Asn Arg Pro Met Asn Pro Met Ile Asn Ala Gly 135 140 145 gcg atc gcc atc aac cag ctg atc aac ggc tcc gat tcc acc gtg gaa 595 Ala Ile Ala Ile Asn Gln Leu Ile Asn Gly Ser Asp Ser Thr Val Glu 150 155 160 165 gac cgc gtg gaa aaa atc cga cac tac ttc tct gaa ctt gct gga cgc 643 Asp Arg Val Glu Lys Ile Arg His Tyr Phe Ser Glu Leu Ala Gly Arg 170 175 180 gaa ctc acc atc gac cgc gtg ctt gcc gaa tcc gaa ctc gcc ggc gcc 691 Glu Leu Thr Ile Asp Arg Val Leu Ala Glu Ser Glu Leu Ala Gly Ala 185 190 195 gac cgc aac ctc tcc atc gcc cac atg ctg cgc aat tac ggc gtc atc 739 Asp Arg Asn Leu Ser Ile Ala His Met Leu Arg Asn Tyr Gly Val Ile 200 205 210 gaa gac gaa gcc cac gac gcc gtc ctc agc tac acg ctg caa tgc gcc 787 Glu Asp Glu Ala His Asp Ala Val Leu Ser Tyr Thr Leu Gln Cys Ala 215 220 225 atc aaa gta acc acg cgc gac ctc gca gtc atg acc gcc acg ctc gcc 835 Ile Lys Val Thr Thr Arg Asp Leu Ala Val Met Thr Ala Thr Leu Ala 230 235 240 245 gcc ggc ggc aca cac cca att acc ggc aag aag ctt ctc gac gcc cgc 883 Ala Gly Gly Thr His Pro Ile Thr Gly Lys Lys Leu Leu Asp Ala Arg 250 255 260 gtc tgc cgc ctc acc ctc tcc gtc atg gct tca gca ggc atg tac gac 931 Val Cys Arg Leu Thr Leu Ser Val Met Ala Ser Ala Gly Met Tyr Asp 265 270 275 gag gca ggg cag tgg ctc tcc acc gta ggc atc ccc gcg aaa tca gga 979 Glu Ala Gly Gln Trp Leu Ser Thr Val Gly Ile Pro Ala Lys Ser Gly 280 285 290 gtc gcc ggc gga ctc atc ggc att ctg cca ggt cag ctg ggc atc gcc 1027 Val Ala Gly Gly Leu Ile Gly Ile Leu Pro Gly Gln Leu Gly Ile Ala 295 300 305 aca ttt tcc cca cgc ctg aac ccc aaa ggc aac agc gtg cgc ggc gta 1075 Thr Phe Ser Pro Arg Leu Asn Pro Lys Gly Asn Ser Val Arg Gly Val 310 315 320 325 aaa ata ttc aaa cag ctt tcc gac gac atg ggc ctc cac ctc atg tcc 1123 Lys Ile Phe Lys Gln Leu Ser Asp Asp Met Gly Leu His Leu Met Ser 330 335 340 acc gag cag gta tcc ggc cac gca gta cga tcc atc acg cgg gac ggc 1171 Thr Glu Gln Val Ser Gly His Ala Val Arg Ser Ile Thr Arg Asp Gly 345 350 355 gac acc acc ttc atc caa atg cag ggc gcc atg aac ttc tcc gcc agc 1219 Asp Thr Thr Phe Ile Gln Met Gln Gly Ala Met Asn Phe Ser Ala Ser 360 365 370 gaa agc ttc ctc cac gcc atc gtg gaa cac aac ttt gaa ggc acc gaa 1267 Glu Ser Phe Leu His Ala Ile Val Glu His Asn Phe Glu Gly Thr Glu 375 380 385 gtt gtt ctt gat ctc acc cga gta ctt agc ttc cac ccc gta gcc atc 1315 Val Val Leu Asp Leu Thr Arg Val Leu Ser Phe His Pro Val Ala Ile 390 395 400 405 cgc atg atc aaa gaa ggc ctc aaa cgc atc cgc gac gca ggc ttt gag 1363 Arg Met Ile Lys Glu Gly Leu Lys Arg Ile Arg Asp Ala Gly Phe Glu 410 415 420 gtg ttc atc ctc gac cca gat gac gta ctg ccc gat ttc atg ttt tcc 1411 Val Phe Ile Leu Asp Pro Asp Asp Val Leu Pro Asp Phe Met Phe Ser 425 430 435 gac ggc acc atc tgc aaa gaa cga gtg tgaccggtag ctttatgggc 1458 Asp Gly Thr Ile Cys Lys Glu Arg Val 440 445 tgaacaattc 1468 <210> 158 <211> 446 <212> PRT <213> Corynebacterium glutamicum <400> 158 Leu Arg His His Lys Trp Trp Leu Leu Ser Arg Cys Glu Pro Gln Asn 1 5 10 15 Leu Ser Cys Thr Arg Val Tyr Ser Ala Lys Glu Ile Thr Ser Leu Asp 20 25 30 Ser Met Leu Thr Met Pro Ile Pro Glu Tyr Leu His Glu Ile Leu Asp 35 40 45 Asp Val Arg Asp Thr Thr Ser Gly Glu Leu Ala Asp Tyr Ile Pro Glu 50 55 60 Leu Lys Ser Ala Asp Pro Asn Pro Leu Ala Val Ala Leu Cys Thr Val 65 70 75 80 Asn Gly His Ile Tyr Ser Ala Gly Asp Asp Asp Ile Glu Phe Thr Met 85 90 95 Gln Ser Ile Ser Lys Pro Phe Ala Tyr Ala Leu Ala Leu Gln Glu Cys 100 105 110 Gly Phe Asp Glu Val Ser Ala Ser Val Ala Leu Glu Pro Ser Gly Glu 115 120 125 Ala Phe Asn Glu Leu Ser Leu Asp Gly Glu Asn Arg Pro Met Asn Pro 130 135 140 Met Ile Asn Ala Gly Ala Ile Ala Ile Asn Gln Leu Ile Asn Gly Ser 145 150 155 160 Asp Ser Thr Val Glu Asp Arg Val Glu Lys Ile Arg His Tyr Phe Ser 165 170 175 Glu Leu Ala Gly Arg Glu Leu Thr Ile Asp Arg Val Leu Ala Glu Ser 180 185 190 Glu Leu Ala Gly Ala Asp Arg Asn Leu Ser Ile Ala His Met Leu Arg 195 200 205 Asn Tyr Gly Val Ile Glu Asp Glu Ala His Asp Ala Val Leu Ser Tyr 210 215 220 Thr Leu Gln Cys Ala Ile Lys Val Thr Thr Arg Asp Leu Ala Val Met 225 230 235 240 Thr Ala Thr Leu Ala Ala Gly Gly Thr His Pro Ile Thr Gly Lys Lys 245 250 255 Leu Leu Asp Ala Arg Val Cys Arg Leu Thr Leu Ser Val Met Ala Ser 260 265 270 Ala Gly Met Tyr Asp Glu Ala Gly Gln Trp Leu Ser Thr Val Gly Ile 275 280 285 Pro Ala Lys Ser Gly Val Ala Gly Gly Leu Ile Gly Ile Leu Pro Gly 290 295 300 Gln Leu Gly Ile Ala Thr Phe Ser Pro Arg Leu Asn Pro Lys Gly Asn 305 310 315 320 Ser Val Arg Gly Val Lys Ile Phe Lys Gln Leu Ser Asp Asp Met Gly 325 330 335 Leu His Leu Met Ser Thr Glu Gln Val Ser Gly His Ala Val Arg Ser 340 345 350 Ile Thr Arg Asp Gly Asp Thr Thr Phe Ile Gln Met Gln Gly Ala Met 355 360 365 Asn Phe Ser Ala Ser Glu Ser Phe Leu His Ala Ile Val Glu His Asn 370 375 380 Phe Glu Gly Thr Glu Val Val Leu Asp Leu Thr Arg Val Leu Ser Phe 385 390 395 400 His Pro Val Ala Ile Arg Met Ile Lys Glu Gly Leu Lys Arg Ile Arg 405 410 415 Asp Ala Gly Phe Glu Val Phe Ile Leu Asp Pro Asp Asp Val Leu Pro 420 425 430 Asp Phe Met Phe Ser Asp Gly Thr Ile Cys Lys Glu Arg Val 435 440 445 <210> 159 <211> 1174 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1144) <223> RXA04360 <400> 159 cagtttcacc ctgagtcagt gctaagccca acgggtcctg tcattttgtc ccgctgtgtc 60 gaacagcttc tcgcgaacta ataaaaaaag gatttgattc atg act tct cca gca 115 Met Thr Ser Pro Ala 1 5 aca ctg aaa gtt ctc aac gcc tac ttg gat aac ccc act cca acc ctg 163 Thr Leu Lys Val Leu Asn Ala Tyr Leu Asp Asn Pro Thr Pro Thr Leu 10 15 20 gag gag gca att gag gtg ttc acc ccg ctg acc gtg ggt gaa tac gat 211 Glu Glu Ala Ile Glu Val Phe Thr Pro Leu Thr Val Gly Glu Tyr Asp 25 30 35 gac gtg cac atc gca gcg ctg ctt gcc acc atc cgt act cgc ggt gag 259 Asp Val His Ile Ala Ala Leu Leu Ala Thr Ile Arg Thr Arg Gly Glu 40 45 50 cag ttc gct gat att gcc ggc gct gcc aag gcg ttc ctc gcg gcg gct 307 Gln Phe Ala Asp Ile Ala Gly Ala Ala Lys Ala Phe Leu Ala Ala Ala 55 60 65 cgt ccg ttc ccg att act ggc gca ggt ttg cta gat tcc gct ggt act 355 Arg Pro Phe Pro Ile Thr Gly Ala Gly Leu Leu Asp Ser Ala Gly Thr 70 75 80 85 ggt ggc gac ggt gcc aac acc atc aac atc acc acc ggc gca tcc ctg 403 Gly Gly Asp Gly Ala Asn Thr Ile Asn Ile Thr Thr Gly Ala Ser Leu 90 95 100 atc gca gca tcc ggt gga gtg aag ctg gtt aag cac ggc aac cgt tcg 451 Ile Ala Ala Ser Gly Gly Val Lys Leu Val Lys His Gly Asn Arg Ser 105 110 115 gtg agc tcc aag tcc ggc tcc gcc gat gtg ctg gaa gcg ctg aat att 499 Val Ser Ser Lys Ser Gly Ser Ala Asp Val Leu Glu Ala Leu Asn Ile 120 125 130 cct ttg ggc ctt gat gtg gat cgt gct gtg aag tgg ttc gaa gcg tcc 547 Pro Leu Gly Leu Asp Val Asp Arg Ala Val Lys Trp Phe Glu Ala Ser 135 140 145 aac ttc acc ttc ctg ttc gca cct gcg tac aac cct gcg att gcg cat 595 Asn Phe Thr Phe Leu Phe Ala Pro Ala Tyr Asn Pro Ala Ile Ala His 150 155 160 165 gtg cag ccg gtt cgc cag gcg ctg aaa ttc ccc acc atc ttc aac acg 643 Val Gln Pro Val Arg Gln Ala Leu Lys Phe Pro Thr Ile Phe Asn Thr 170 175 180 ctt gga cca ttg ctg tcc ccg gcg cgc ccg gag cgt cag atc atg ggc 691 Leu Gly Pro Leu Leu Ser Pro Ala Arg Pro Glu Arg Gln Ile Met Gly 185 190 195 gtg gcc aat gcc aat cat gga cag ctc atc gcc gag gtc ttc cgc gag 739 Val Ala Asn Ala Asn His Gly Gln Leu Ile Ala Glu Val Phe Arg Glu 200 205 210 ttg ggc cgt aca cgc gcg ctt gtt gtg cat ggc gca ggc acc gat gag 787 Leu Gly Arg Thr Arg Ala Leu Val Val His Gly Ala Gly Thr Asp Glu 215 220 225 atc gca gtc cac ggc acc acc ttg gtg tgg gag ctt aaa gaa gac ggc 835 Ile Ala Val His Gly Thr Thr Leu Val Trp Glu Leu Lys Glu Asp Gly 230 235 240 245 acc atc gag cat tac acc atc gag cct gag gac ctt ggc ctt ggc cgc 883 Thr Ile Glu His Tyr Thr Ile Glu Pro Glu Asp Leu Gly Leu Gly Arg 250 255 260 tac acc ctt gag gat ctc gta ggt ggc ctc ggc act gag aac gcc gaa 931 Tyr Thr Leu Glu Asp Leu Val Gly Gly Leu Gly Thr Glu Asn Ala Glu 265 270 275 gct atg cgc gct act ttc gcg ggc acc ggc cct gat gca cac cgt gat 979 Ala Met Arg Ala Thr Phe Ala Gly Thr Gly Pro Asp Ala His Arg Asp 280 285 290 gcg ttg gct gcg tcc gca ggt gcg atg ttc tac ctc aac ggc gat gtc 1027 Ala Leu Ala Ala Ser Ala Gly Ala Met Phe Tyr Leu Asn Gly Asp Val 295 300 305 gac tcc ttg aaa gat ggt gca caa aag gcg ctt tcc ttg ctt gcc gac 1075 Asp Ser Leu Lys Asp Gly Ala Gln Lys Ala Leu Ser Leu Leu Ala Asp 310 315 320 325 ggc acc acc cag gca tgg ttg gcc aag cac gaa gag atc gat tac tca 1123 Gly Thr Thr Gln Ala Trp Leu Ala Lys His Glu Glu Ile Asp Tyr Ser 330 335 340 gaa aag gag tct tcc aat gac tagtaataat ctgcccacag tgttggaaag 1174 Glu Lys Glu Ser Ser Asn Asp 345 <210> 160 <211> 348 <212> PRT <213> Corynebacterium glutamicum <400> 160 Met Thr Ser Pro Ala Thr Leu Lys Val Leu Asn Ala Tyr Leu Asp Asn 1 5 10 15 Pro Thr Pro Thr Leu Glu Glu Ala Ile Glu Val Phe Thr Pro Leu Thr 20 25 30 Val Gly Glu Tyr Asp Asp Val His Ile Ala Ala Leu Leu Ala Thr Ile 35 40 45 Arg Thr Arg Gly Glu Gln Phe Ala Asp Ile Ala Gly Ala Ala Lys Ala 50 55 60 Phe Leu Ala Ala Ala Arg Pro Phe Pro Ile Thr Gly Ala Gly Leu Leu 65 70 75 80 Asp Ser Ala Gly Thr Gly Gly Asp Gly Ala Asn Thr Ile Asn Ile Thr 85 90 95 Thr Gly Ala Ser Leu Ile Ala Ala Ser Gly Gly Val Lys Leu Val Lys 100 105 110 His Gly Asn Arg Ser Val Ser Ser Lys Ser Gly Ser Ala Asp Val Leu 115 120 125 Glu Ala Leu Asn Ile Pro Leu Gly Leu Asp Val Asp Arg Ala Val Lys 130 135 140 Trp Phe Glu Ala Ser Asn Phe Thr Phe Leu Phe Ala Pro Ala Tyr Asn 145 150 155 160 Pro Ala Ile Ala His Val Gln Pro Val Arg Gln Ala Leu Lys Phe Pro 165 170 175 Thr Ile Phe Asn Thr Leu Gly Pro Leu Leu Ser Pro Ala Arg Pro Glu 180 185 190 Arg Gln Ile Met Gly Val Ala Asn Ala Asn His Gly Gln Leu Ile Ala 195 200 205 Glu Val Phe Arg Glu Leu Gly Arg Thr Arg Ala Leu Val Val His Gly 210 215 220 Ala Gly Thr Asp Glu Ile Ala Val His Gly Thr Thr Leu Val Trp Glu 225 230 235 240 Leu Lys Glu Asp Gly Thr Ile Glu His Tyr Thr Ile Glu Pro Glu Asp 245 250 255 Leu Gly Leu Gly Arg Tyr Thr Leu Glu Asp Leu Val Gly Gly Leu Gly 260 265 270 Thr Glu Asn Ala Glu Ala Met Arg Ala Thr Phe Ala Gly Thr Gly Pro 275 280 285 Asp Ala His Arg Asp Ala Leu Ala Ala Ser Ala Gly Ala Met Phe Tyr 290 295 300 Leu Asn Gly Asp Val Asp Ser Leu Lys Asp Gly Ala Gln Lys Ala Leu 305 310 315 320 Ser Leu Leu Ala Asp Gly Thr Thr Gln Ala Trp Leu Ala Lys His Glu 325 330 335 Glu Ile Asp Tyr Ser Glu Lys Glu Ser Ser Asn Asp 340 345 <210> 161 <211> 4530 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(4530) <223> RXA07000 <400> 161 atg aaa cca caa gga ctc tac aac cct gcg cat gaa cat gac gcc tgc 48 Met Lys Pro Gln Gly Leu Tyr Asn Pro Ala His Glu His Asp Ala Cys 1 5 10 15 ggt gtg gcg ttt att gcg gat atc cac ggt cga ccc agc cgc agc att 96 Gly Val Ala Phe Ile Ala Asp Ile His Gly Arg Pro Ser Arg Ser Ile 20 25 30 gtt gat cgt gca ctt gag gcg ctt cgc aac att gac cac cga ggt gcc 144 Val Asp Arg Ala Leu Glu Ala Leu Arg Asn Ile Asp His Arg Gly Ala 35 40 45 gcc ggt gca gag aag aac act ggc gat ggt gcg ggc atc ctc atg cag 192 Ala Gly Ala Glu Lys Asn Thr Gly Asp Gly Ala Gly Ile Leu Met Gln 50 55 60 att ccg gac ggc ttt tat cgt gaa gta tct ggc att gag ctt cct gag 240 Ile Pro Asp Gly Phe Tyr Arg Glu Val Ser Gly Ile Glu Leu Pro Glu 65 70 75 80 gca ggg gag tat gcc act ggt att gcg ttc ttg cct cgc ggt cgc atg 288 Ala Gly Glu Tyr Ala Thr Gly Ile Ala Phe Leu Pro Arg Gly Arg Met 85 90 95 gcg atg atg gat gct cag aag gaa att gag cgc atc gca aag caa gaa 336 Ala Met Met Asp Ala Gln Lys Glu Ile Glu Arg Ile Ala Lys Gln Glu 100 105 110 ggt gcc gat gtg ctt ggt tgg cgc atg gtt cct ttt gat tct cgt gat 384 Gly Ala Asp Val Leu Gly Trp Arg Met Val Pro Phe Asp Ser Arg Asp 115 120 125 ttg ggt tcc atg gct gag gag gcg atg cct agt ttc gcg cag att ttc 432 Leu Gly Ser Met Ala Glu Glu Ala Met Pro Ser Phe Ala Gln Ile Phe 130 135 140 ctt act gtg cct gga aaa tct ggt gaa gat ctt gac cgt gtg atg ttc 480 Leu Thr Val Pro Gly Lys Ser Gly Glu Asp Leu Asp Arg Val Met Phe 145 150 155 160 ttt atc cgt aag cgt tgt gag cgt gag ctg ggc acc acc aat ggt cgc 528 Phe Ile Arg Lys Arg Cys Glu Arg Glu Leu Gly Thr Thr Asn Gly Arg 165 170 175 gat acg gtg tat ttc ccg tcg cta tct tca cgc acc atc att tac aaa 576 Asp Thr Val Tyr Phe Pro Ser Leu Ser Ser Arg Thr Ile Ile Tyr Lys 180 185 190 ggc atg ttg acc act ctg cag ctt gag ggc ttc ttt gag gat ctg ggt 624 Gly Met Leu Thr Thr Leu Gln Leu Glu Gly Phe Phe Glu Asp Leu Gly 195 200 205 gat gct cgc ctg gag tcg gcc att gct att gtg cac tcg cgt ttc tcc 672 Asp Ala Arg Leu Glu Ser Ala Ile Ala Ile Val His Ser Arg Phe Ser 210 215 220 acg aac act ttc cca agc tgg ccg ctg gcg cac ccg tac cgt ttc gtt 720 Thr Asn Thr Phe Pro Ser Trp Pro Leu Ala His Pro Tyr Arg Phe Val 225 230 235 240 gcc cac aac ggt gag atc aac act gtg cgt ggc aat gaa aac tgg atg 768 Ala His Asn Gly Glu Ile Asn Thr Val Arg Gly Asn Glu Asn Trp Met 245 250 255 cgc gcc cgc gag gcg ctt atc aaa aac gac aag ctg ggc aat ttg agc 816 Arg Ala Arg Glu Ala Leu Ile Lys Asn Asp Lys Leu Gly Asn Leu Ser 260 265 270 agc gtg ctg cct atc tgc acc ccg gag ggc tcg gat acc gcg cgt ttc 864 Ser Val Leu Pro Ile Cys Thr Pro Glu Gly Ser Asp Thr Ala Arg Phe 275 280 285 gac gag gct ttg gag ctt ttg cac ctg ggc gga tac tca ctt ccg cat 912 Asp Glu Ala Leu Glu Leu Leu His Leu Gly Gly Tyr Ser Leu Pro His 290 295 300 gct gtt gcg atg atg atc cct cag gcg tgg gaa cac aac aag acg ctg 960 Ala Val Ala Met Met Ile Pro Gln Ala Trp Glu His Asn Lys Thr Leu 305 310 315 320 agc cct gag ctg cgt gat ttc tac gaa tac cac tct tgt ctg atg gag 1008 Ser Pro Glu Leu Arg Asp Phe Tyr Glu Tyr His Ser Cys Leu Met Glu 325 330 335 cca tgg gat ggt cct gca gcg ctg gca ttt act gac ggt cgt ttt gtg 1056 Pro Trp Asp Gly Pro Ala Ala Leu Ala Phe Thr Asp Gly Arg Phe Val 340 345 350 ggt gcc gtg ctg gac cgt aat ggc ctg cga cct ggg cga atc acc att 1104 Gly Ala Val Leu Asp Arg Asn Gly Leu Arg Pro Gly Arg Ile Thr Ile 355 360 365 act gat tcg ggt ttg gtt gtg atg gct tct gaa tcg gga gtg ttg gac 1152 Thr Asp Ser Gly Leu Val Val Met Ala Ser Glu Ser Gly Val Leu Asp 370 375 380 ttg agg gag gag agc gtc gta aag cgt act cgc gta cag cct gga cgc 1200 Leu Arg Glu Glu Ser Val Val Lys Arg Thr Arg Val Gln Pro Gly Arg 385 390 395 400 atg ttc ctt gtt gac act gcc gag ggc cgc atc gtt gaa gac gag gaa 1248 Met Phe Leu Val Asp Thr Ala Glu Gly Arg Ile Val Glu Asp Glu Glu 405 410 415 atc aag cag aaa tta agc gaa gcg cag cca tat ggt gag tgg att cgc 1296 Ile Lys Gln Lys Leu Ser Glu Ala Gln Pro Tyr Gly Glu Trp Ile Arg 420 425 430 gat aat ttt gtg cat ctg gat cgt ctg cct cag aca cgc tac aac tac 1344 Asp Asn Phe Val His Leu Asp Arg Leu Pro Gln Thr Arg Tyr Asn Tyr 435 440 445 atg gcg cac tct cgt gct gtg ttg cgt cag cgt gtt ttc gga atc act 1392 Met Ala His Ser Arg Ala Val Leu Arg Gln Arg Val Phe Gly Ile Thr 450 455 460 gaa gaa gat gtg gat ttg ttg ctg ctg ccg atg gcc cgc cag ggt gct 1440 Glu Glu Asp Val Asp Leu Leu Leu Leu Pro Met Ala Arg Gln Gly Ala 465 470 475 480 gag gcg att ggt tcc atg ggt tcg gat acg cca att gcg gcg cta tcc 1488 Glu Ala Ile Gly Ser Met Gly Ser Asp Thr Pro Ile Ala Ala Leu Ser 485 490 495 cag cga cca cgc atg ctt tat gat ttc ttc gcg cag cgc ttt gct cag 1536 Gln Arg Pro Arg Met Leu Tyr Asp Phe Phe Ala Gln Arg Phe Ala Gln 500 505 510 gtg aca aac cca ccg ttg gac tct atc cgc gaa aag cct gtg acc agc 1584 Val Thr Asn Pro Pro Leu Asp Ser Ile Arg Glu Lys Pro Val Thr Ser 515 520 525 atg ttc act ttg ttg ggt gcg cag tct gac gtg ctc aat ccg ggt cct 1632 Met Phe Thr Leu Leu Gly Ala Gln Ser Asp Val Leu Asn Pro Gly Pro 530 535 540 gat gcg gcg cga cgt atc cgt ttg gaa tcg ccg atc att gat aac cat 1680 Asp Ala Ala Arg Arg Ile Arg Leu Glu Ser Pro Ile Ile Asp Asn His 545 550 555 560 gag ctg gcc acc ttg atc aat gcc aac gcg cat ggt gag tgg gat tcc 1728 Glu Leu Ala Thr Leu Ile Asn Ala Asn Ala His Gly Glu Trp Asp Ser 565 570 575 ttt ggt gct gct gta att tct ggt ttg tac cca gtg gct cac cat ggt 1776 Phe Gly Ala Ala Val Ile Ser Gly Leu Tyr Pro Val Ala His His Gly 580 585 590 gcc ggc atg aag gct gcg att gct cgt gtg cgc cgc gag gtt tct gaa 1824 Ala Gly Met Lys Ala Ala Ile Ala Arg Val Arg Arg Glu Val Ser Glu 595 600 605 gca atc cgc aat ggc aag acg ttg atc gtg ctg tcg gat cgt gaa tct 1872 Ala Ile Arg Asn Gly Lys Thr Leu Ile Val Leu Ser Asp Arg Glu Ser 610 615 620 gat gag cgc atg gca cct atc cct gcg ctg ctg ctg act tcc gct gtg 1920 Asp Glu Arg Met Ala Pro Ile Pro Ala Leu Leu Leu Thr Ser Ala Val 625 630 635 640 cat cag tac ttg gtg cag caa cgt acc cgt acc cag tgc tcc ctg gtg 1968 His Gln Tyr Leu Val Gln Gln Arg Thr Arg Thr Gln Cys Ser Leu Val 645 650 655 gtg gaa tcc ggc gat gcc cgc gag gtt cat cac ctg gcg atg ctc att 2016 Val Glu Ser Gly Asp Ala Arg Glu Val His His Leu Ala Met Leu Ile 660 665 670 ggt ttt ggt gcc gat gcg atc aac ccg tac atg gca ttt gaa acc atc 2064 Gly Phe Gly Ala Asp Ala Ile Asn Pro Tyr Met Ala Phe Glu Thr Ile 675 680 685 gat gag ctg cgc atg aag ggt cag ttg ggt gat ctt tct ttg gat gag 2112 Asp Glu Leu Arg Met Lys Gly Gln Leu Gly Asp Leu Ser Leu Asp Glu 690 695 700 gca tcc cga aac tac atc aag gca gcc acc act ggt gtg ctg aag gtg 2160 Ala Ser Arg Asn Tyr Ile Lys Ala Ala Thr Thr Gly Val Leu Lys Val 705 710 715 720 atg tcc aag atg ggc att gca acg gtg tct tcg tac cgt ggc gcg cag 2208 Met Ser Lys Met Gly Ile Ala Thr Val Ser Ser Tyr Arg Gly Ala Gln 725 730 735 ctt gcc gat gtc act ggt ctg cac cag gat ctc ctg gac aac tac ttc 2256 Leu Ala Asp Val Thr Gly Leu His Gln Asp Leu Leu Asp Asn Tyr Phe 740 745 750 ggt ggt att gct tca cca att tct ggc atc ggt ctg gat gaa gtt gca 2304 Gly Gly Ile Ala Ser Pro Ile Ser Gly Ile Gly Leu Asp Glu Val Ala 755 760 765 gct gac gta gaa gct cgt cac cgc agc gca ttt ttg cca cgc cct gaa 2352 Ala Asp Val Glu Ala Arg His Arg Ser Ala Phe Leu Pro Arg Pro Glu 770 775 780 gag cac gct cac cgt gaa ttg gat ttg ggt ggt gaa tac aag tgg cgc 2400 Glu His Ala His Arg Glu Leu Asp Leu Gly Gly Glu Tyr Lys Trp Arg 785 790 795 800 cgc gaa ggt gaa tac cac ctg ttc aac cca gaa acc atc ttc aag ctg 2448 Arg Glu Gly Glu Tyr His Leu Phe Asn Pro Glu Thr Ile Phe Lys Leu 805 810 815 cag cat gca acg cgt tct ggc agc tac gag att ttc aag gat tac acc 2496 Gln His Ala Thr Arg Ser Gly Ser Tyr Glu Ile Phe Lys Asp Tyr Thr 820 825 830 cgc aag gtt gat gat caa tcc act cgc ttg ggt act att cgt gga ctg 2544 Arg Lys Val Asp Asp Gln Ser Thr Arg Leu Gly Thr Ile Arg Gly Leu 835 840 845 ttt gag ttc agc acg gac cgc aag cca att tcg gtg tct gag gtg gag 2592 Phe Glu Phe Ser Thr Asp Arg Lys Pro Ile Ser Val Ser Glu Val Glu 850 855 860 ccg gtc agt gag atc gtg aag cgt ttc tcc act ggt gcg atg tct tat 2640 Pro Val Ser Glu Ile Val Lys Arg Phe Ser Thr Gly Ala Met Ser Tyr 865 870 875 880 ggc tcg att tct gct gaa gcc cat gag gtc ttg gcc atc gcc atg aac 2688 Gly Ser Ile Ser Ala Glu Ala His Glu Val Leu Ala Ile Ala Met Asn 885 890 895 cga ctg ggc ggt atg tcc aac tcc ggc gaa ggt ggc gag gac gcc cgc 2736 Arg Leu Gly Gly Met Ser Asn Ser Gly Glu Gly Gly Glu Asp Ala Arg 900 905 910 cga ttt gat gtg gaa ccc aac ggt gac tgg aag cgc tct gcc att aag 2784 Arg Phe Asp Val Glu Pro Asn Gly Asp Trp Lys Arg Ser Ala Ile Lys 915 920 925 cag gtg gcc tcg gga cgt ttc ggc gtg acc agc cac tac ttg aac aac 2832 Gln Val Ala Ser Gly Arg Phe Gly Val Thr Ser His Tyr Leu Asn Asn 930 935 940 tgc acc gat att cag atc aag atg gca cag ggc gca aag ccc ggt gaa 2880 Cys Thr Asp Ile Gln Ile Lys Met Ala Gln Gly Ala Lys Pro Gly Glu 945 950 955 960 ggt ggc cag ctg cca cca aac aag gtg tac cca tgg gtt gca gaa gtc 2928 Gly Gly Gln Leu Pro Pro Asn Lys Val Tyr Pro Trp Val Ala Glu Val 965 970 975 cgc atc acc acc cca ggc gtt ggt ctg att tcc cct cca cca cac cac 2976 Arg Ile Thr Thr Pro Gly Val Gly Leu Ile Ser Pro Pro Pro His His 980 985 990 gat att tac tcc att gag gat ctg gct cag ctg atc cac gac ctg aag 3024 Asp Ile Tyr Ser Ile Glu Asp Leu Ala Gln Leu Ile His Asp Leu Lys 995 1000 1005 aac gct aac cca cgc gca cga atc cac gtg aag cta gtg gca gaa caa 3072 Asn Ala Asn Pro Arg Ala Arg Ile His Val Lys Leu Val Ala Glu Gln 1010 1015 1020 ggc gtg ggc acc gtt gcc gca ggt gtg tcc aaa gca cac gct gat gtg 3120 Gly Val Gly Thr Val Ala Ala Gly Val Ser Lys Ala His Ala Asp Val 1025 1030 1035 1040 gtg ctt att tcc ggc cac gat ggc gga act ggc gca tct cct ttg acc 3168 Val Leu Ile Ser Gly His Asp Gly Gly Thr Gly Ala Ser Pro Leu Thr 1045 1050 1055 tcc ctg aag cat gcc ggt ggt cca tgg gag ttg ggc ttg gct gaa acc 3216 Ser Leu Lys His Ala Gly Gly Pro Trp Glu Leu Gly Leu Ala Glu Thr 1060 1065 1070 cag caa acg ttg ctg ctc aac ggc ctg cgc gat cgt att cgc gtg cag 3264 Gln Gln Thr Leu Leu Leu Asn Gly Leu Arg Asp Arg Ile Arg Val Gln 1075 1080 1085 tgc gat ggt cag ctg aaa act ggc cga gac gtg gtt atc gca gct ctt 3312 Cys Asp Gly Gln Leu Lys Thr Gly Arg Asp Val Val Ile Ala Ala Leu 1090 1095 1100 ctc ggt gcc gaa gaa ttc ggt ttt gcc acc gca ccg ctg gtg gtt gaa 3360 Leu Gly Ala Glu Glu Phe Gly Phe Ala Thr Ala Pro Leu Val Val Glu 1105 1110 1115 1120 ggc tgc atc atg atg cgc gtc tgc cac ctg gac acc tgc ccg gtg ggt 3408 Gly Cys Ile Met Met Arg Val Cys His Leu Asp Thr Cys Pro Val Gly 1125 1130 1135 atc gct acc cag aac ccg gat ttg cgt tcc aag ttc acc ggc aag gct 3456 Ile Ala Thr Gln Asn Pro Asp Leu Arg Ser Lys Phe Thr Gly Lys Ala 1140 1145 1150 gaa cac gtg gtc aac ttc ttc acc ttc atc gcc cag gaa gtc cgt gag 3504 Glu His Val Val Asn Phe Phe Thr Phe Ile Ala Gln Glu Val Arg Glu 1155 1160 1165 tac ttg gca cag ctt ggt ttc cgc tct att gat gaa gcc gtc gga caa 3552 Tyr Leu Ala Gln Leu Gly Phe Arg Ser Ile Asp Glu Ala Val Gly Gln 1170 1175 1180 gcc cag gtg ctg cgc aag cgt tcc gga atc cca gct gat tcc cgc gca 3600 Ala Gln Val Leu Arg Lys Arg Ser Gly Ile Pro Ala Asp Ser Arg Ala 1185 1190 1195 1200 gca cac ctg gat ttg agc cca att ttc cat cgc cca gaa act cca cac 3648 Ala His Leu Asp Leu Ser Pro Ile Phe His Arg Pro Glu Thr Pro His 1205 1210 1215 ttc cca act cag gat gtg cgt tgc acc aag acc cag gaa cac agc cta 3696 Phe Pro Thr Gln Asp Val Arg Cys Thr Lys Thr Gln Glu His Ser Leu 1220 1225 1230 gaa aaa gcc ctg gac aac gca ttt att gat aag gct tcg gac acg atc 3744 Glu Lys Ala Leu Asp Asn Ala Phe Ile Asp Lys Ala Ser Asp Thr Ile 1235 1240 1245 acc cgt gcc gca gcg ggt gtg gaa acc agc att gtt att gat agc tcc 3792 Thr Arg Ala Ala Ala Gly Val Glu Thr Ser Ile Val Ile Asp Ser Ser 1250 1255 1260 atc agc aac gtc aac cgt tca gtt ggc acg atg ctg ggt tct gca gtc 3840 Ile Ser Asn Val Asn Arg Ser Val Gly Thr Met Leu Gly Ser Ala Val 1265 1270 1275 1280 agc cgc gtg gct ggt gcc caa ggt ttg cca gac ggc acc atc acc ttg 3888 Ser Arg Val Ala Gly Ala Gln Gly Leu Pro Asp Gly Thr Ile Thr Leu 1285 1290 1295 aat ctt caa ggc tgc gcc ggt aac tcc ttt ggc gcg ttc atc cca cga 3936 Asn Leu Gln Gly Cys Ala Gly Asn Ser Phe Gly Ala Phe Ile Pro Arg 1300 1305 1310 ggc atc acc atc aac ctc acc ggc gat gcc aat gac ttt gtg ggc aag 3984 Gly Ile Thr Ile Asn Leu Thr Gly Asp Ala Asn Asp Phe Val Gly Lys 1315 1320 1325 gga tta tct ggc gga aag att gtg atc aag cct tcc gct cag gct ccg 4032 Gly Leu Ser Gly Gly Lys Ile Val Ile Lys Pro Ser Ala Gln Ala Pro 1330 1335 1340 aag cag ctg aag aac aat cca aat atc att gcc gga aac gtg ctt gga 4080 Lys Gln Leu Lys Asn Asn Pro Asn Ile Ile Ala Gly Asn Val Leu Gly 1345 1350 1355 1360 tac ggc gca acc agt ggt gaa ttg ttc att cgt ggc cag gtc ggc gaa 4128 Tyr Gly Ala Thr Ser Gly Glu Leu Phe Ile Arg Gly Gln Val Gly Glu 1365 1370 1375 cgt ttc tgc gtc cgt aac tct ggc gcc acc gca gtg gtt gaa ggt atc 4176 Arg Phe Cys Val Arg Asn Ser Gly Ala Thr Ala Val Val Glu Gly Ile 1380 1385 1390 gga aac cac ggt tgt gag tac atg act ggc ggc cga gtc ctg gtt ttg 4224 Gly Asn His Gly Cys Glu Tyr Met Thr Gly Gly Arg Val Leu Val Leu 1395 1400 1405 ggc ccg gtt ggt gag aac ttt ggt gcc ggc atg tct ggt ggc att gca 4272 Gly Pro Val Gly Glu Asn Phe Gly Ala Gly Met Ser Gly Gly Ile Ala 1410 1415 1420 tac ctg gct aat tcc ccg gac cta aac cag aag atc aat ggc gaa ttg 4320 Tyr Leu Ala Asn Ser Pro Asp Leu Asn Gln Lys Ile Asn Gly Glu Leu 1425 1430 1435 1440 gtg gat gtt gtt cca ctg agc gct gac gat ctg acg tgg gct gat gag 4368 Val Asp Val Val Pro Leu Ser Ala Asp Asp Leu Thr Trp Ala Asp Glu 1445 1450 1455 ctc att gct cgc cac cgc gaa ctc acc gga tcc gag acc aag ctg cgt 4416 Leu Ile Ala Arg His Arg Glu Leu Thr Gly Ser Glu Thr Lys Leu Arg 1460 1465 1470 gca caa gat ttg gtg aaa atc atg ccg cgc gat ttc caa aaa gta ctc 4464 Ala Gln Asp Leu Val Lys Ile Met Pro Arg Asp Phe Gln Lys Val Leu 1475 1480 1485 aac atc atc gaa acg gcc cac gct gag ggc caa gac cca gca atc aag 4512 Asn Ile Ile Glu Thr Ala His Ala Glu Gly Gln Asp Pro Ala Ile Lys 1490 1495 1500 atc atg gag gca gtg agc 4530 Ile Met Glu Ala Val Ser 1505 1510 <210> 162 <211> 1510 <212> PRT <213> Corynebacterium glutamicum <400> 162 Met Lys Pro Gln Gly Leu Tyr Asn Pro Ala His Glu His Asp Ala Cys 1 5 10 15 Gly Val Ala Phe Ile Ala Asp Ile His Gly Arg Pro Ser Arg Ser Ile 20 25 30 Val Asp Arg Ala Leu Glu Ala Leu Arg Asn Ile Asp His Arg Gly Ala 35 40 45 Ala Gly Ala Glu Lys Asn Thr Gly Asp Gly Ala Gly Ile Leu Met Gln 50 55 60 Ile Pro Asp Gly Phe Tyr Arg Glu Val Ser Gly Ile Glu Leu Pro Glu 65 70 75 80 Ala Gly Glu Tyr Ala Thr Gly Ile Ala Phe Leu Pro Arg Gly Arg Met 85 90 95 Ala Met Met Asp Ala Gln Lys Glu Ile Glu Arg Ile Ala Lys Gln Glu 100 105 110 Gly Ala Asp Val Leu Gly Trp Arg Met Val Pro Phe Asp Ser Arg Asp 115 120 125 Leu Gly Ser Met Ala Glu Glu Ala Met Pro Ser Phe Ala Gln Ile Phe 130 135 140 Leu Thr Val Pro Gly Lys Ser Gly Glu Asp Leu Asp Arg Val Met Phe 145 150 155 160 Phe Ile Arg Lys Arg Cys Glu Arg Glu Leu Gly Thr Thr Asn Gly Arg 165 170 175 Asp Thr Val Tyr Phe Pro Ser Leu Ser Ser Arg Thr Ile Ile Tyr Lys 180 185 190 Gly Met Leu Thr Thr Leu Gln Leu Glu Gly Phe Phe Glu Asp Leu Gly 195 200 205 Asp Ala Arg Leu Glu Ser Ala Ile Ala Ile Val His Ser Arg Phe Ser 210 215 220 Thr Asn Thr Phe Pro Ser Trp Pro Leu Ala His Pro Tyr Arg Phe Val 225 230 235 240 Ala His Asn Gly Glu Ile Asn Thr Val Arg Gly Asn Glu Asn Trp Met 245 250 255 Arg Ala Arg Glu Ala Leu Ile Lys Asn Asp Lys Leu Gly Asn Leu Ser 260 265 270 Ser Val Leu Pro Ile Cys Thr Pro Glu Gly Ser Asp Thr Ala Arg Phe 275 280 285 Asp Glu Ala Leu Glu Leu Leu His Leu Gly Gly Tyr Ser Leu Pro His 290 295 300 Ala Val Ala Met Met Ile Pro Gln Ala Trp Glu His Asn Lys Thr Leu 305 310 315 320 Ser Pro Glu Leu Arg Asp Phe Tyr Glu Tyr His Ser Cys Leu Met Glu 325 330 335 Pro Trp Asp Gly Pro Ala Ala Leu Ala Phe Thr Asp Gly Arg Phe Val 340 345 350 Gly Ala Val Leu Asp Arg Asn Gly Leu Arg Pro Gly Arg Ile Thr Ile 355 360 365 Thr Asp Ser Gly Leu Val Val Met Ala Ser Glu Ser Gly Val Leu Asp 370 375 380 Leu Arg Glu Glu Ser Val Val Lys Arg Thr Arg Val Gln Pro Gly Arg 385 390 395 400 Met Phe Leu Val Asp Thr Ala Glu Gly Arg Ile Val Glu Asp Glu Glu 405 410 415 Ile Lys Gln Lys Leu Ser Glu Ala Gln Pro Tyr Gly Glu Trp Ile Arg 420 425 430 Asp Asn Phe Val His Leu Asp Arg Leu Pro Gln Thr Arg Tyr Asn Tyr 435 440 445 Met Ala His Ser Arg Ala Val Leu Arg Gln Arg Val Phe Gly Ile Thr 450 455 460 Glu Glu Asp Val Asp Leu Leu Leu Leu Pro Met Ala Arg Gln Gly Ala 465 470 475 480 Glu Ala Ile Gly Ser Met Gly Ser Asp Thr Pro Ile Ala Ala Leu Ser 485 490 495 Gln Arg Pro Arg Met Leu Tyr Asp Phe Phe Ala Gln Arg Phe Ala Gln 500 505 510 Val Thr Asn Pro Pro Leu Asp Ser Ile Arg Glu Lys Pro Val Thr Ser 515 520 525 Met Phe Thr Leu Leu Gly Ala Gln Ser Asp Val Leu Asn Pro Gly Pro 530 535 540 Asp Ala Ala Arg Arg Ile Arg Leu Glu Ser Pro Ile Ile Asp Asn His 545 550 555 560 Glu Leu Ala Thr Leu Ile Asn Ala Asn Ala His Gly Glu Trp Asp Ser 565 570 575 Phe Gly Ala Ala Val Ile Ser Gly Leu Tyr Pro Val Ala His His Gly 580 585 590 Ala Gly Met Lys Ala Ala Ile Ala Arg Val Arg Arg Glu Val Ser Glu 595 600 605 Ala Ile Arg Asn Gly Lys Thr Leu Ile Val Leu Ser Asp Arg Glu Ser 610 615 620 Asp Glu Arg Met Ala Pro Ile Pro Ala Leu Leu Leu Thr Ser Ala Val 625 630 635 640 His Gln Tyr Leu Val Gln Gln Arg Thr Arg Thr Gln Cys Ser Leu Val 645 650 655 Val Glu Ser Gly Asp Ala Arg Glu Val His His Leu Ala Met Leu Ile 660 665 670 Gly Phe Gly Ala Asp Ala Ile Asn Pro Tyr Met Ala Phe Glu Thr Ile 675 680 685 Asp Glu Leu Arg Met Lys Gly Gln Leu Gly Asp Leu Ser Leu Asp Glu 690 695 700 Ala Ser Arg Asn Tyr Ile Lys Ala Ala Thr Thr Gly Val Leu Lys Val 705 710 715 720 Met Ser Lys Met Gly Ile Ala Thr Val Ser Ser Tyr Arg Gly Ala Gln 725 730 735 Leu Ala Asp Val Thr Gly Leu His Gln Asp Leu Leu Asp Asn Tyr Phe 740 745 750 Gly Gly Ile Ala Ser Pro Ile Ser Gly Ile Gly Leu Asp Glu Val Ala 755 760 765 Ala Asp Val Glu Ala Arg His Arg Ser Ala Phe Leu Pro Arg Pro Glu 770 775 780 Glu His Ala His Arg Glu Leu Asp Leu Gly Gly Glu Tyr Lys Trp Arg 785 790 795 800 Arg Glu Gly Glu Tyr His Leu Phe Asn Pro Glu Thr Ile Phe Lys Leu 805 810 815 Gln His Ala Thr Arg Ser Gly Ser Tyr Glu Ile Phe Lys Asp Tyr Thr 820 825 830 Arg Lys Val Asp Asp Gln Ser Thr Arg Leu Gly Thr Ile Arg Gly Leu 835 840 845 Phe Glu Phe Ser Thr Asp Arg Lys Pro Ile Ser Val Ser Glu Val Glu 850 855 860 Pro Val Ser Glu Ile Val Lys Arg Phe Ser Thr Gly Ala Met Ser Tyr 865 870 875 880 Gly Ser Ile Ser Ala Glu Ala His Glu Val Leu Ala Ile Ala Met Asn 885 890 895 Arg Leu Gly Gly Met Ser Asn Ser Gly Glu Gly Gly Glu Asp Ala Arg 900 905 910 Arg Phe Asp Val Glu Pro Asn Gly Asp Trp Lys Arg Ser Ala Ile Lys 915 920 925 Gln Val Ala Ser Gly Arg Phe Gly Val Thr Ser His Tyr Leu Asn Asn 930 935 940 Cys Thr Asp Ile Gln Ile Lys Met Ala Gln Gly Ala Lys Pro Gly Glu 945 950 955 960 Gly Gly Gln Leu Pro Pro Asn Lys Val Tyr Pro Trp Val Ala Glu Val 965 970 975 Arg Ile Thr Thr Pro Gly Val Gly Leu Ile Ser Pro Pro Pro His His 980 985 990 Asp Ile Tyr Ser Ile Glu Asp Leu Ala Gln Leu Ile His Asp Leu Lys 995 1000 1005 Asn Ala Asn Pro Arg Ala Arg Ile His Val Lys Leu Val Ala Glu Gln 1010 1015 1020 Gly Val Gly Thr Val Ala Ala Gly Val Ser Lys Ala His Ala Asp Val 1025 1030 1035 1040 Val Leu Ile Ser Gly His Asp Gly Gly Thr Gly Ala Ser Pro Leu Thr 1045 1050 1055 Ser Leu Lys His Ala Gly Gly Pro Trp Glu Leu Gly Leu Ala Glu Thr 1060 1065 1070 Gln Gln Thr Leu Leu Leu Asn Gly Leu Arg Asp Arg Ile Arg Val Gln 1075 1080 1085 Cys Asp Gly Gln Leu Lys Thr Gly Arg Asp Val Val Ile Ala Ala Leu 1090 1095 1100 Leu Gly Ala Glu Glu Phe Gly Phe Ala Thr Ala Pro Leu Val Val Glu 1105 1110 1115 1120 Gly Cys Ile Met Met Arg Val Cys His Leu Asp Thr Cys Pro Val Gly 1125 1130 1135 Ile Ala Thr Gln Asn Pro Asp Leu Arg Ser Lys Phe Thr Gly Lys Ala 1140 1145 1150 Glu His Val Val Asn Phe Phe Thr Phe Ile Ala Gln Glu Val Arg Glu 1155 1160 1165 Tyr Leu Ala Gln Leu Gly Phe Arg Ser Ile Asp Glu Ala Val Gly Gln 1170 1175 1180 Ala Gln Val Leu Arg Lys Arg Ser Gly Ile Pro Ala Asp Ser Arg Ala 1185 1190 1195 1200 Ala His Leu Asp Leu Ser Pro Ile Phe His Arg Pro Glu Thr Pro His 1205 1210 1215 Phe Pro Thr Gln Asp Val Arg Cys Thr Lys Thr Gln Glu His Ser Leu 1220 1225 1230 Glu Lys Ala Leu Asp Asn Ala Phe Ile Asp Lys Ala Ser Asp Thr Ile 1235 1240 1245 Thr Arg Ala Ala Ala Gly Val Glu Thr Ser Ile Val Ile Asp Ser Ser 1250 1255 1260 Ile Ser Asn Val Asn Arg Ser Val Gly Thr Met Leu Gly Ser Ala Val 1265 1270 1275 1280 Ser Arg Val Ala Gly Ala Gln Gly Leu Pro Asp Gly Thr Ile Thr Leu 1285 1290 1295 Asn Leu Gln Gly Cys Ala Gly Asn Ser Phe Gly Ala Phe Ile Pro Arg 1300 1305 1310 Gly Ile Thr Ile Asn Leu Thr Gly Asp Ala Asn Asp Phe Val Gly Lys 1315 1320 1325 Gly Leu Ser Gly Gly Lys Ile Val Ile Lys Pro Ser Ala Gln Ala Pro 1330 1335 1340 Lys Gln Leu Lys Asn Asn Pro Asn Ile Ile Ala Gly Asn Val Leu Gly 1345 1350 1355 1360 Tyr Gly Ala Thr Ser Gly Glu Leu Phe Ile Arg Gly Gln Val Gly Glu 1365 1370 1375 Arg Phe Cys Val Arg Asn Ser Gly Ala Thr Ala Val Val Glu Gly Ile 1380 1385 1390 Gly Asn His Gly Cys Glu Tyr Met Thr Gly Gly Arg Val Leu Val Leu 1395 1400 1405 Gly Pro Val Gly Glu Asn Phe Gly Ala Gly Met Ser Gly Gly Ile Ala 1410 1415 1420 Tyr Leu Ala Asn Ser Pro Asp Leu Asn Gln Lys Ile Asn Gly Glu Leu 1425 1430 1435 1440 Val Asp Val Val Pro Leu Ser Ala Asp Asp Leu Thr Trp Ala Asp Glu 1445 1450 1455 Leu Ile Ala Arg His Arg Glu Leu Thr Gly Ser Glu Thr Lys Leu Arg 1460 1465 1470 Ala Gln Asp Leu Val Lys Ile Met Pro Arg Asp Phe Gln Lys Val Leu 1475 1480 1485 Asn Ile Ile Glu Thr Ala His Ala Glu Gly Gln Asp Pro Ala Ile Lys 1490 1495 1500 Ile Met Glu Ala Val Ser 1505 1510 <210> 163 <211> 1455 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1455) <223> RXA07001 <400> 163 atg aag tac ttg gtg gcg ctg gga aga agc ctt cgt tat ggg agg tct 48 Met Lys Tyr Leu Val Ala Leu Gly Arg Ser Leu Arg Tyr Gly Arg Ser 1 5 10 15 ccc aga cac aat cga ata cgg gcc gga tat cca tct cgg ctc atc acc 96 Pro Arg His Asn Arg Ile Arg Ala Gly Tyr Pro Ser Arg Leu Ile Thr 20 25 30 ccg ctt ttt atc aag aaa gat gag gac ctc ttg aag cgt ctt act cgc 144 Pro Leu Phe Ile Lys Lys Asp Glu Asp Leu Leu Lys Arg Leu Thr Arg 35 40 45 atc gca tcc atc agc atg gcc tcc atg ctc gcc gcc gca agt ctc gtc 192 Ile Ala Ser Ile Ser Met Ala Ser Met Leu Ala Ala Ala Ser Leu Val 50 55 60 gcg tgc tcc ggc tcc acc gac gag gaa ggc gat gtt tac ttc ctg aac 240 Ala Cys Ser Gly Ser Thr Asp Glu Glu Gly Asp Val Tyr Phe Leu Asn 65 70 75 80 ttc aag cct gaa cag gac gtg gca tac cag gaa atc gca aag gcc tac 288 Phe Lys Pro Glu Gln Asp Val Ala Tyr Gln Glu Ile Ala Lys Ala Tyr 85 90 95 act gaa gag acc ggc gtt aag gtc aag gtc gtt act gcc gcc tcc ggc 336 Thr Glu Glu Thr Gly Val Lys Val Lys Val Val Thr Ala Ala Ser Gly 100 105 110 tcc tat gag cag acc ctc aag gcc gag att ggc aag gac gaa gcc ccg 384 Ser Tyr Glu Gln Thr Leu Lys Ala Glu Ile Gly Lys Asp Glu Ala Pro 115 120 125 act ctc ttc cag gtc aat ggc cca gcc ggc ttc atc act tgg cag gac 432 Thr Leu Phe Gln Val Asn Gly Pro Ala Gly Phe Ile Thr Trp Gln Asp 130 135 140 tac atg gca gat atg tcg gac acc gag gta gct aag cag ctg acc gac 480 Tyr Met Ala Asp Met Ser Asp Thr Glu Val Ala Lys Gln Leu Thr Asp 145 150 155 160 gac att ccg ccg ctg acc acc gag gat ggc gag gta cgt ggc gtt ccg 528 Asp Ile Pro Pro Leu Thr Thr Glu Asp Gly Glu Val Arg Gly Val Pro 165 170 175 ttc gcc gtc gag ggc ttc ggc atc atc tac aac gac gag atc ttc gac 576 Phe Ala Val Glu Gly Phe Gly Ile Ile Tyr Asn Asp Glu Ile Phe Asp 180 185 190 aag tac atc gcc acg tcc ggc gca aag atc aag tcc acg gat gag atc 624 Lys Tyr Ile Ala Thr Ser Gly Ala Lys Ile Lys Ser Thr Asp Glu Ile 195 200 205 acg agc tac cag aag ctc aag gaa gtc gcc gag gat atg cag gca aag 672 Thr Ser Tyr Gln Lys Leu Lys Glu Val Ala Glu Asp Met Gln Ala Lys 210 215 220 aag gac gag ctc ggt atc gaa ggc gcc ttc gcc tcc acc tcg ctg aca 720 Lys Asp Glu Leu Gly Ile Glu Gly Ala Phe Ala Ser Thr Ser Leu Thr 225 230 235 240 tcc ggt gag gac tgg cgt tgg cag acc cac ctg gcc aac gct ccg atc 768 Ser Gly Glu Asp Trp Arg Trp Gln Thr His Leu Ala Asn Ala Pro Ile 245 250 255 tgg cag gag tac cag gac aag gga gtc gag gac acc aac gag atc gag 816 Trp Gln Glu Tyr Gln Asp Lys Gly Val Glu Asp Thr Asn Glu Ile Glu 260 265 270 ttc tcc tac aac aag gag tac aag aac ctt ttc gat ctc tac ctt gag 864 Phe Ser Tyr Asn Lys Glu Tyr Lys Asn Leu Phe Asp Leu Tyr Leu Glu 275 280 285 aac tcc acc gta gaa aag tct ctt gcg ccg tct aag acg gtg tct gat 912 Asn Ser Thr Val Glu Lys Ser Leu Ala Pro Ser Lys Thr Val Ser Asp 290 295 300 tcc atg gct gag ttc gca cag ggc aag gcc gct atg gtt cag aac ggt 960 Ser Met Ala Glu Phe Ala Gln Gly Lys Ala Ala Met Val Gln Asn Gly 305 310 315 320 aac tgg gca tgg tcc cag att tcc gag act tct ggc aac gtg gtc aag 1008 Asn Trp Ala Trp Ser Gln Ile Ser Glu Thr Ser Gly Asn Val Val Lys 325 330 335 gaa gac aag atc aag ttc ctg ccc atg tac atg ggt ctg cca gat gaa 1056 Glu Asp Lys Ile Lys Phe Leu Pro Met Tyr Met Gly Leu Pro Asp Glu 340 345 350 gaa aag cac ggc atc aac gtc ggt acc gag aac tat ttg ggc gtg aac 1104 Glu Lys His Gly Ile Asn Val Gly Thr Glu Asn Tyr Leu Gly Val Asn 355 360 365 tct gag gcc tcc gag gtc gac cag cag gcc acc aag gac ttc gtg gat 1152 Ser Glu Ala Ser Glu Val Asp Gln Gln Ala Thr Lys Asp Phe Val Asp 370 375 380 tgg ctg ttt acc tct gaa gct ggc aag gag cac gtg gtg aag gac ctt 1200 Trp Leu Phe Thr Ser Glu Ala Gly Lys Glu His Val Val Lys Asp Leu 385 390 395 400 ggc ttc atc gca ccg ttc gaa agc tac acc gct gag aac acc ccg aat 1248 Gly Phe Ile Ala Pro Phe Glu Ser Tyr Thr Ala Glu Asn Thr Pro Asn 405 410 415 gac ccc ctt tct gag caa gtc gcg gaa gct atc gct aac aag gat ctg 1296 Asp Pro Leu Ser Glu Gln Val Ala Glu Ala Ile Ala Asn Lys Asp Leu 420 425 430 acc acc tac ccg tgg aac ttc cag tac ttc ccg tcc cag cag ttc aag 1344 Thr Thr Tyr Pro Trp Asn Phe Gln Tyr Phe Pro Ser Gln Gln Phe Lys 435 440 445 gat gac ttc ggc cag gat ctg tcg cag tac gcc tcc gga aag ctg aag 1392 Asp Asp Phe Gly Gln Asp Leu Ser Gln Tyr Ala Ser Gly Lys Leu Lys 450 455 460 tgg gag gac gtc gtc aag cac ttc aat gac aac tgg gct gct gag aag 1440 Trp Glu Asp Val Val Lys His Phe Asn Asp Asn Trp Ala Ala Glu Lys 465 470 475 480 gaa tcc aac tgg ggc 1455 Glu Ser Asn Trp Gly 485 <210> 164 <211> 485 <212> PRT <213> Corynebacterium glutamicum <400> 164 Met Lys Tyr Leu Val Ala Leu Gly Arg Ser Leu Arg Tyr Gly Arg Ser 1 5 10 15 Pro Arg His Asn Arg Ile Arg Ala Gly Tyr Pro Ser Arg Leu Ile Thr 20 25 30 Pro Leu Phe Ile Lys Lys Asp Glu Asp Leu Leu Lys Arg Leu Thr Arg 35 40 45 Ile Ala Ser Ile Ser Met Ala Ser Met Leu Ala Ala Ala Ser Leu Val 50 55 60 Ala Cys Ser Gly Ser Thr Asp Glu Glu Gly Asp Val Tyr Phe Leu Asn 65 70 75 80 Phe Lys Pro Glu Gln Asp Val Ala Tyr Gln Glu Ile Ala Lys Ala Tyr 85 90 95 Thr Glu Glu Thr Gly Val Lys Val Lys Val Val Thr Ala Ala Ser Gly 100 105 110 Ser Tyr Glu Gln Thr Leu Lys Ala Glu Ile Gly Lys Asp Glu Ala Pro 115 120 125 Thr Leu Phe Gln Val Asn Gly Pro Ala Gly Phe Ile Thr Trp Gln Asp 130 135 140 Tyr Met Ala Asp Met Ser Asp Thr Glu Val Ala Lys Gln Leu Thr Asp 145 150 155 160 Asp Ile Pro Pro Leu Thr Thr Glu Asp Gly Glu Val Arg Gly Val Pro 165 170 175 Phe Ala Val Glu Gly Phe Gly Ile Ile Tyr Asn Asp Glu Ile Phe Asp 180 185 190 Lys Tyr Ile Ala Thr Ser Gly Ala Lys Ile Lys Ser Thr Asp Glu Ile 195 200 205 Thr Ser Tyr Gln Lys Leu Lys Glu Val Ala Glu Asp Met Gln Ala Lys 210 215 220 Lys Asp Glu Leu Gly Ile Glu Gly Ala Phe Ala Ser Thr Ser Leu Thr 225 230 235 240 Ser Gly Glu Asp Trp Arg Trp Gln Thr His Leu Ala Asn Ala Pro Ile 245 250 255 Trp Gln Glu Tyr Gln Asp Lys Gly Val Glu Asp Thr Asn Glu Ile Glu 260 265 270 Phe Ser Tyr Asn Lys Glu Tyr Lys Asn Leu Phe Asp Leu Tyr Leu Glu 275 280 285 Asn Ser Thr Val Glu Lys Ser Leu Ala Pro Ser Lys Thr Val Ser Asp 290 295 300 Ser Met Ala Glu Phe Ala Gln Gly Lys Ala Ala Met Val Gln Asn Gly 305 310 315 320 Asn Trp Ala Trp Ser Gln Ile Ser Glu Thr Ser Gly Asn Val Val Lys 325 330 335 Glu Asp Lys Ile Lys Phe Leu Pro Met Tyr Met Gly Leu Pro Asp Glu 340 345 350 Glu Lys His Gly Ile Asn Val Gly Thr Glu Asn Tyr Leu Gly Val Asn 355 360 365 Ser Glu Ala Ser Glu Val Asp Gln Gln Ala Thr Lys Asp Phe Val Asp 370 375 380 Trp Leu Phe Thr Ser Glu Ala Gly Lys Glu His Val Val Lys Asp Leu 385 390 395 400 Gly Phe Ile Ala Pro Phe Glu Ser Tyr Thr Ala Glu Asn Thr Pro Asn 405 410 415 Asp Pro Leu Ser Glu Gln Val Ala Glu Ala Ile Ala Asn Lys Asp Leu 420 425 430 Thr Thr Tyr Pro Trp Asn Phe Gln Tyr Phe Pro Ser Gln Gln Phe Lys 435 440 445 Asp Asp Phe Gly Gln Asp Leu Ser Gln Tyr Ala Ser Gly Lys Leu Lys 450 455 460 Trp Glu Asp Val Val Lys His Phe Asn Asp Asn Trp Ala Ala Glu Lys 465 470 475 480 Glu Ser Asn Trp Gly 485

Claims (8)

1. 서열번호 10의 아미노산 서열을 갖는 폴리펩티드를 코딩하며, 144번째 아미노산 위치에서 아스파르트산을 코딩하는 단리된 핵산 분자.
2. 삭제
3. 제1항에 따른 핵산 서열을 포함하는 벡터.
4. 제3항에 따른 벡터로 형질감염된 숙주 세포.
5. 제4항에 있어서, 상기 핵산 분자의 발현에 의해 리신의 생산이 조정되는 숙주 세포.
6. 제3항에 따른 벡터로 형질감염된 세포를 배양하여 리신을 생산하는 단계를 포함하는 리신의 제조 방법.
7. 삭제
8. 삭제