CN1296492C - 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 - Google Patents

一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 Download PDF

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Publication number
CN1296492C
CN1296492C CNB2004100904233A CN200410090423A CN1296492C CN 1296492 C CN1296492 C CN 1296492C CN B2004100904233 A CNB2004100904233 A CN B2004100904233A CN 200410090423 A CN200410090423 A CN 200410090423A CN 1296492 C CN1296492 C CN 1296492C
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China
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nucleic acid
sequence
capture probe
overhang
acid capture
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Expired - Fee Related
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CNB2004100904233A
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English (en)
Chinese (zh)
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CN1635165A (zh
Inventor
邵威
赵永超
孙义民
乔继英
魏华江
杨卫平
周玉祥
程京
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Tsinghua University
CapitalBio Corp
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Tsinghua University
CapitalBio Corp
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Priority to CNB2004100904233A priority Critical patent/CN1296492C/zh
Application filed by Tsinghua University, CapitalBio Corp filed Critical Tsinghua University
Priority to JP2007541638A priority patent/JP4664373B2/ja
Priority to PCT/CN2004/001340 priority patent/WO2006053463A1/zh
Priority to CA002586408A priority patent/CA2586408A1/en
Priority to AT04797371T priority patent/ATE545030T1/de
Priority to US11/791,157 priority patent/US8680016B2/en
Priority to AU2004324960A priority patent/AU2004324960A1/en
Priority to EP04797371A priority patent/EP1816476B1/en
Publication of CN1635165A publication Critical patent/CN1635165A/zh
Application granted granted Critical
Publication of CN1296492C publication Critical patent/CN1296492C/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB2004100904233A 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 Expired - Fee Related CN1296492C (zh)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CNB2004100904233A CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法
PCT/CN2004/001340 WO2006053463A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
CA002586408A CA2586408A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
AT04797371T ATE545030T1 (de) 2004-11-18 2004-11-23 Testverfahren für nukleinsäurebindendes protein auf biochipbasis
JP2007541638A JP4664373B2 (ja) 2004-11-18 2004-11-23 バイオチップをベースにした核酸結合タンパク質の試験方法
US11/791,157 US8680016B2 (en) 2004-11-18 2004-11-23 Testing method of nucleic acid binding protein based on biochip
AU2004324960A AU2004324960A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
EP04797371A EP1816476B1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2004100904233A CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法

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CN1635165A CN1635165A (zh) 2005-07-06
CN1296492C true CN1296492C (zh) 2007-01-24

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US (1) US8680016B2 (enExample)
EP (1) EP1816476B1 (enExample)
JP (1) JP4664373B2 (enExample)
CN (1) CN1296492C (enExample)
AT (1) ATE545030T1 (enExample)
AU (1) AU2004324960A1 (enExample)
CA (1) CA2586408A1 (enExample)
WO (1) WO2006053463A1 (enExample)

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CA2659745A1 (en) 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
US20090053732A1 (en) 2007-07-16 2009-02-26 Ophir Vermesh Microfluidic devices, methods and systems for detecting target molecules
EP2274443A4 (en) * 2008-04-09 2012-03-28 California Inst Of Techn MUG AND PROCESSES AND SYSTEMS ASSOCIATED WITH DETECTING AND / OR SORTING TARGETS
KR101090982B1 (ko) 2009-03-16 2011-12-08 경희대학교 산학협력단 양자점-앱타머 접합체를 이용한 생분석 방법
US8741559B2 (en) * 2009-04-24 2014-06-03 Discoverx Corporation Cellular assay employing detectable protein
DK3489685T3 (da) 2011-11-21 2023-12-04 Zoetis Services Llc Signalforstærkning i sidestrømning og tilsvarende immunoassays
CN103048463A (zh) * 2012-05-02 2013-04-17 中国科学院广州生物医药与健康研究院 一种基于不同严谨度的用于检测dna结合蛋白的微孔板核酸杂交elisa方法
JP6197039B2 (ja) 2012-08-24 2017-09-13 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ ハイスループット多重検出用システム、装置及び方法
WO2015060511A1 (ko) * 2013-10-22 2015-04-30 주식회사 바이오이즈 생체분자와 핵산의 결합정보를 생성하기 위한 마커, 이의 제조방법, 이를 이용한 생체분자 분석방법 및 장치
TWI691716B (zh) 2014-08-13 2020-04-21 美商艾巴希斯公司 電漿特異性結合搭配物檢定中之信號放大
WO2016090148A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of cell secretion profiles
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
JP6944438B2 (ja) 2015-08-04 2021-10-06 ゾエティス サービシズ リミテッド ライアビリティ カンパニー 溶液ベースのプラズモン特異的結合パートナーアッセイにおけるシグナル増幅
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法，以及其制造方法
KR102654470B1 (ko) 2017-01-30 2024-04-05 조에티스 서비시즈 엘엘씨 용액-기반 플라스몬 특이적-결합 파트너 검정 및 금속성 나노구조체
EP3963070A4 (en) * 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
EP4644564A1 (en) * 2022-12-28 2025-11-05 BGI Shenzhen Detection composition and protein capture detection method

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WO2002014550A2 (en) * 2000-08-14 2002-02-21 Transgenetics Incorporated Transcription factor target gene discovery
CN1435492A (zh) * 2003-03-05 2003-08-13 东南大学 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
WO2004087919A1 (ja) * 2003-03-28 2004-10-14 National Institute Of Advanced Industrial Science And Technology マイクロアレイを用いたアプタマーの取得方法

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AU3595493A (en) * 1992-01-28 1993-09-01 President And Fellors Of Harvard College Method for nucleic acid hybridization using single-stranded DNA binding protein
US20030054378A1 (en) * 1997-05-08 2003-03-20 Isao Karube Method for detecting target nucleotide sequence
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
CA2327561A1 (en) * 2000-12-27 2002-06-27 Pierre Rousseau Methods for the screening and/or for the identification of differentially active nucleic acid binding factors of nucleic acid binding elements using dna microarray technology (dpa):dna proteomic array
US20020177218A1 (en) * 2001-03-30 2002-11-28 Yu Fang Methods of detecting multiple DNA binding protein and DNA interactions in a sample, and devices, systems and kits for practicing the same
AU2002305585A1 (en) * 2001-04-23 2002-11-05 Michael F. Chou Transcription factor network discovery methods
AU2003256814A1 (en) * 2002-07-26 2004-02-16 Alfa Wasserman, Inc. Macromolecular protection assay
AU2003295692A1 (en) * 2002-11-15 2004-06-15 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
US20040115794A1 (en) * 2002-12-12 2004-06-17 Affymetrix, Inc. Methods for detecting transcriptional factor binding sites

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
WO2002014550A2 (en) * 2000-08-14 2002-02-21 Transgenetics Incorporated Transcription factor target gene discovery
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
CN1435492A (zh) * 2003-03-05 2003-08-13 东南大学 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
WO2004087919A1 (ja) * 2003-03-28 2004-10-14 National Institute Of Advanced Industrial Science And Technology マイクロアレイを用いたアプタマーの取得方法

Also Published As

Publication number Publication date
EP1816476A1 (en) 2007-08-08
CN1635165A (zh) 2005-07-06
JP2008520212A (ja) 2008-06-19
AU2004324960A1 (en) 2006-05-26
US20090018025A1 (en) 2009-01-15
EP1816476B1 (en) 2012-02-08
US8680016B2 (en) 2014-03-25
EP1816476A4 (en) 2008-08-20
CA2586408A1 (en) 2006-05-26
JP4664373B2 (ja) 2011-04-06
ATE545030T1 (de) 2012-02-15
WO2006053463A1 (en) 2006-05-26

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