CN1296492C - 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 - Google Patents
一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 Download PDFInfo
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- CN1296492C CN1296492C CNB2004100904233A CN200410090423A CN1296492C CN 1296492 C CN1296492 C CN 1296492C CN B2004100904233 A CNB2004100904233 A CN B2004100904233A CN 200410090423 A CN200410090423 A CN 200410090423A CN 1296492 C CN1296492 C CN 1296492C
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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Abstract
Description
组编号 | 转录因子 | 探针编号 | 探针名称 | 探针序列 |
1 | AP-1 | 1 | AP-1-IP | 5′-T20-CGCTTGA TGAGTCAGCCGGA-TCCCG-3′ |
2 | AP-1-LP | 5′-biotin-CGGGA-TCCGGC TGACTCATCAAGCG-3′ | ||
3 | AP-1-FP | 5′-CGCTTGA TGAGTCAGCCGGA-3′ |
4 | AP-1-CP | 5′-TCCGGC TGACTCATCAAGCG-3′ | ||
2 | NFκB | 5 | NFκB-IP | 5′-T20-AGTTGAG GGGACTTTCCCAGGA-TCCCG-3′ |
6 | NFκB-LP | 5′-biotin-CGGGA-TCCTG GGAAAGTCCCCTCAACT-3′ | ||
7 | NFκB-FP | 5′-AGTTGAG GGGACTTTCCCAGGA-3′ | ||
8 | NFκB-CP | 5′-TCCTG GGAAAGTCCCCTCAACT-3′ | ||
3 | SP1 | 9 | SP1-IP | 5′-T20-AAAGC CCCGCCCCGATATAAT-TCCCG-3′ |
10 | SP1-LP | 5′-biotin-CGGGA-ATTATATC GGGGCGGGGCTTT-3′ | ||
11 | SP1-FP | 5′-AAAGC CCCGCCCCGATATAAT-3′ | ||
12 | SP1-CP | 5′-ATTATATC GGGGCGGGGCTTT-3′ | ||
4 | TFIID | 13 | TFIID-IP | 5′-T20-CGCCTACCTCATT TTATATGCTCTGC-TCCCG-3′ |
14 | TFIID-LP | 5′-biotin-CGGGA-GCAGAGCA TATAAAATGAGGTAGGCG-3′ | ||
15 | TFIID-FP | 5′-CGCCTACCTCATT TTATATGCTCTGC-3′ | ||
16 | TFIID-CP | 5′-GCAGAGCA TATAAAATGAGGTAGGCG-3′ | ||
5 | NC | 17 | NC-IP | 5′-T20-CTATGTGGTGAACTCCTCCTAAATA-TCCCG-3′ |
18 | NC-LP | 5′-biotin-CGGGA-CGGGATATTTAGGAGGAGTTTCACCACATAG-3′ | ||
19 | NC-FP | 5′-CTATGTGGTGAACTCCTCCTAAATA-3′ | ||
6 | HC | 20 | HC-IP | 5′-T20-AGACGGAAGACATATGGCCGCTC-TCCCG-3′ |
21 | HC-LP | 5′-biotin-CGGGA-GAGCGGCCATATGTCTTCCGTCT-3′ |
组编号 | TF | 探针编号 | 探针名称 | 探针序列 |
1 | AP-1 | 1 | AP-1-IP | 5′-T20-CGCTTGAT GAGTCAGCCGGA-TCCCG-3′ |
22 | AP-1-LFP | 5′-CGCTTGA TGAGTCAGCCGGA-CCCTATAGTGAGTCGTATTACCCC-3′ | ||
26 | AP-1-LP’ | 5′-CGGGA-TCCGGC TGACTTCATCAAGCG-3′ | ||
2 | NFκB | 5 | NFκB-IP | 5′-T20-AGTTGAG GGGACTTTCCCAGGA-TCCCG-3′ |
23 | NFκB-LFP | 5′-AGTTGAG GGGACTTTCCCAGGA-CCCTATAGTGAGTCGTATTACCCCC-3′ | ||
27 | NFκB-LP’ | 5′-CGGGA-TCCTG GGAAAGTCCCCTCAACT-3′ | ||
3 | SP1 | 9 | SP1-IP | 5′-T20-AAAGC CCCGCCCCGATATAAT-TCCCG-3′ |
24 | SP1-LFP | 5′-AAAGC CCCGCCCCGATATAAT-CCCTATAGTGAGTCGTATTACCCC-3′ | ||
28 | SP1-LP’ | 5′-CGGGA-ATTATATC GGGGCGGGGCTTT-3′ | ||
4 | 25 | T7 Pro | 5’-Cy3-GGGGTAATACGACTCACTAAGGG-3’ |
Claims (24)
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100904233A CN1296492C (zh) | 2004-11-18 | 2004-11-18 | 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 |
JP2007541638A JP4664373B2 (ja) | 2004-11-18 | 2004-11-23 | バイオチップをベースにした核酸結合タンパク質の試験方法 |
AT04797371T ATE545030T1 (de) | 2004-11-18 | 2004-11-23 | Testverfahren für nukleinsäurebindendes protein auf biochipbasis |
CA002586408A CA2586408A1 (en) | 2004-11-18 | 2004-11-23 | A testing method of nucleic acid binding protein based on biochip |
PCT/CN2004/001340 WO2006053463A1 (fr) | 2004-11-18 | 2004-11-23 | Procede de test de proteine de liaison aux acides nucleiques |
AU2004324960A AU2004324960A1 (en) | 2004-11-18 | 2004-11-23 | A testing method of nucleic acid binding protein based on biochip |
US11/791,157 US8680016B2 (en) | 2004-11-18 | 2004-11-23 | Testing method of nucleic acid binding protein based on biochip |
EP04797371A EP1816476B1 (en) | 2004-11-18 | 2004-11-23 | A testing method of nucleic acid binding protein based on biochip |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100904233A CN1296492C (zh) | 2004-11-18 | 2004-11-18 | 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1635165A CN1635165A (zh) | 2005-07-06 |
CN1296492C true CN1296492C (zh) | 2007-01-24 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CNB2004100904233A Expired - Fee Related CN1296492C (zh) | 2004-11-18 | 2004-11-18 | 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US8680016B2 (zh) |
EP (1) | EP1816476B1 (zh) |
JP (1) | JP4664373B2 (zh) |
CN (1) | CN1296492C (zh) |
AT (1) | ATE545030T1 (zh) |
AU (1) | AU2004324960A1 (zh) |
CA (1) | CA2586408A1 (zh) |
WO (1) | WO2006053463A1 (zh) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008016680A1 (en) | 2006-08-02 | 2008-02-07 | California Institute Of Technology | Methods and systems for detecting and/or sorting targets |
US8394590B2 (en) | 2006-08-02 | 2013-03-12 | California Institute Of Technology | Capture agents and related methods and systems for detecting and/or sorting targets |
JP2010533869A (ja) | 2007-07-16 | 2010-10-28 | カリフォルニア インスティチュート オブ テクノロジー | 標的分子を検出するためのマイクロ流体装置、方法及びシステム |
AU2009234162A1 (en) * | 2008-04-09 | 2009-10-15 | California Institute Of Technology | Capture agents and related methods and systems for detecting and/or sorting targets |
KR101090982B1 (ko) | 2009-03-16 | 2011-12-08 | 경희대학교 산학협력단 | 양자점-앱타머 접합체를 이용한 생분석 방법 |
CA2759211C (en) * | 2009-04-24 | 2017-02-28 | Daniel Kelly Treiber | Assay for detecting protein by a cellular process |
RS58168B1 (sr) | 2011-11-21 | 2019-03-29 | Abay Sa | Imunološko ispitivanje pojačavanjem signala |
CN103048463A (zh) * | 2012-05-02 | 2013-04-17 | 中国科学院广州生物医药与健康研究院 | 一种基于不同严谨度的用于检测dna结合蛋白的微孔板核酸杂交elisa方法 |
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JP2008520212A (ja) | 2008-06-19 |
ATE545030T1 (de) | 2012-02-15 |
CA2586408A1 (en) | 2006-05-26 |
CN1635165A (zh) | 2005-07-06 |
AU2004324960A1 (en) | 2006-05-26 |
US8680016B2 (en) | 2014-03-25 |
EP1816476B1 (en) | 2012-02-08 |
EP1816476A1 (en) | 2007-08-08 |
WO2006053463A1 (fr) | 2006-05-26 |
JP4664373B2 (ja) | 2011-04-06 |
US20090018025A1 (en) | 2009-01-15 |
EP1816476A4 (en) | 2008-08-20 |
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