CA2586408A1 - A testing method of nucleic acid binding protein based on biochip - Google Patents

A testing method of nucleic acid binding protein based on biochip Download PDF

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Publication number
CA2586408A1
CA2586408A1 CA002586408A CA2586408A CA2586408A1 CA 2586408 A1 CA2586408 A1 CA 2586408A1 CA 002586408 A CA002586408 A CA 002586408A CA 2586408 A CA2586408 A CA 2586408A CA 2586408 A1 CA2586408 A1 CA 2586408A1
Authority
CA
Canada
Prior art keywords
nucleic acid
overhang
sequence
acid capture
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002586408A
Other languages
English (en)
French (fr)
Inventor
Wei Shao
Yongchao Zhao
Yimin Sun
Jiying Qiao
Huajiang Wei
Weiping Yang
Yuxiang Zhou
Jing Cheng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tsinghua University
CapitalBio Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2586408A1 publication Critical patent/CA2586408A1/en
Abandoned legal-status Critical Current

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002586408A 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip Abandoned CA2586408A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN200410090423.3 2004-11-18
CNB2004100904233A CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法
PCT/CN2004/001340 WO2006053463A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip

Publications (1)

Publication Number Publication Date
CA2586408A1 true CA2586408A1 (en) 2006-05-26

Family

ID=34847597

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002586408A Abandoned CA2586408A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip

Country Status (8)

Country Link
US (1) US8680016B2 (enExample)
EP (1) EP1816476B1 (enExample)
JP (1) JP4664373B2 (enExample)
CN (1) CN1296492C (enExample)
AT (1) ATE545030T1 (enExample)
AU (1) AU2004324960A1 (enExample)
CA (1) CA2586408A1 (enExample)
WO (1) WO2006053463A1 (enExample)

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Publication number Priority date Publication date Assignee Title
CA2659745A1 (en) 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
US20090053732A1 (en) 2007-07-16 2009-02-26 Ophir Vermesh Microfluidic devices, methods and systems for detecting target molecules
EP2274443A4 (en) * 2008-04-09 2012-03-28 California Inst Of Techn MUG AND PROCESSES AND SYSTEMS ASSOCIATED WITH DETECTING AND / OR SORTING TARGETS
KR101090982B1 (ko) 2009-03-16 2011-12-08 경희대학교 산학협력단 양자점-앱타머 접합체를 이용한 생분석 방법
US8741559B2 (en) * 2009-04-24 2014-06-03 Discoverx Corporation Cellular assay employing detectable protein
DK3489685T3 (da) 2011-11-21 2023-12-04 Zoetis Services Llc Signalforstærkning i sidestrømning og tilsvarende immunoassays
CN103048463A (zh) * 2012-05-02 2013-04-17 中国科学院广州生物医药与健康研究院 一种基于不同严谨度的用于检测dna结合蛋白的微孔板核酸杂交elisa方法
JP6197039B2 (ja) 2012-08-24 2017-09-13 イェール ユニバーシティーYale University ハイスループット多重検出用システム、装置及び方法
WO2015060511A1 (ko) * 2013-10-22 2015-04-30 주식회사 바이오이즈 생체분자와 핵산의 결합정보를 생성하기 위한 마커, 이의 제조방법, 이를 이용한 생체분자 분석방법 및 장치
TWI691716B (zh) 2014-08-13 2020-04-21 美商艾巴希斯公司 電漿特異性結合搭配物檢定中之信號放大
WO2016090148A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of cell secretion profiles
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
JP6944438B2 (ja) 2015-08-04 2021-10-06 ゾエティス サービシズ リミテッド ライアビリティ カンパニー 溶液ベースのプラズモン特異的結合パートナーアッセイにおけるシグナル増幅
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法,以及其制造方法
KR102654470B1 (ko) 2017-01-30 2024-04-05 조에티스 서비시즈 엘엘씨 용액-기반 플라스몬 특이적-결합 파트너 검정 및 금속성 나노구조체
EP3963070A4 (en) * 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
EP4644564A1 (en) * 2022-12-28 2025-11-05 BGI Shenzhen Detection composition and protein capture detection method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
AU3595493A (en) * 1992-01-28 1993-09-01 President And Fellors Of Harvard College Method for nucleic acid hybridization using single-stranded DNA binding protein
US20030054378A1 (en) * 1997-05-08 2003-03-20 Isao Karube Method for detecting target nucleotide sequence
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20060292560A1 (en) * 2000-08-14 2006-12-28 Robert Burgess Transcription factor target gene discovery
CA2327561A1 (en) * 2000-12-27 2002-06-27 Pierre Rousseau Methods for the screening and/or for the identification of differentially active nucleic acid binding factors of nucleic acid binding elements using dna microarray technology (dpa):dna proteomic array
US20020177218A1 (en) * 2001-03-30 2002-11-28 Yu Fang Methods of detecting multiple DNA binding protein and DNA interactions in a sample, and devices, systems and kits for practicing the same
AU2002305585A1 (en) * 2001-04-23 2002-11-05 Michael F. Chou Transcription factor network discovery methods
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
AU2003256814A1 (en) * 2002-07-26 2004-02-16 Alfa Wasserman, Inc. Macromolecular protection assay
AU2003295692A1 (en) * 2002-11-15 2004-06-15 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
US20040115794A1 (en) * 2002-12-12 2004-06-17 Affymetrix, Inc. Methods for detecting transcriptional factor binding sites
CN1435492A (zh) * 2003-03-05 2003-08-13 东南大学 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
JP3944576B2 (ja) * 2003-03-28 2007-07-11 独立行政法人産業技術総合研究所 マイクロアレイを用いたアプタマーの取得方法

Also Published As

Publication number Publication date
EP1816476A1 (en) 2007-08-08
CN1635165A (zh) 2005-07-06
JP2008520212A (ja) 2008-06-19
AU2004324960A1 (en) 2006-05-26
US20090018025A1 (en) 2009-01-15
EP1816476B1 (en) 2012-02-08
US8680016B2 (en) 2014-03-25
EP1816476A4 (en) 2008-08-20
CN1296492C (zh) 2007-01-24
JP4664373B2 (ja) 2011-04-06
ATE545030T1 (de) 2012-02-15
WO2006053463A1 (en) 2006-05-26

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Legal Events

Date Code Title Description
FZDE Discontinued