CN1279918C - 补充能量的糖源及其应用 - Google Patents
补充能量的糖源及其应用 Download PDFInfo
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- CN1279918C CN1279918C CNB2004100384046A CN200410038404A CN1279918C CN 1279918 C CN1279918 C CN 1279918C CN B2004100384046 A CNB2004100384046 A CN B2004100384046A CN 200410038404 A CN200410038404 A CN 200410038404A CN 1279918 C CN1279918 C CN 1279918C
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Abstract
通过包括使非还原糖生成酶作用于具有还原能力的部分淀粉水解物这一步骤的方法制备的海藻糖,有利地用作补充能量糖源并作为有效成份用于补充能量组合物中。这种糖源和组合物适合于用作给生物体补充能量的药物组合物而不用担心产生副作用。
Description
本发明是1994年3月16日递交的申请号为94102934.4,发明名称为“补充能量的糖源及其应用”的中国专利申请的分案申请。
技术领域
本发明涉及一种补充能量的糖源及其应用,更详细地说,涉及基本上由海藻糖组成的一种补充能量的糖源,该海藻糖是通过包含使非还原糖生成酶作用于具有还原能力的部分淀粉水解产物这一步骤在内的方法制备的,以及涉及到包含该海藻糖作为有效成分的补充能量组合物。
背景技术
具有还原能力的糖,象葡萄糖和果糖,长时间被用作补充能量的糖源。然而,这些糖由于其还原能力而具有相对差的储存稳定性,而且,一般情况下与象氨基酸和维生素之类的其它营养品共存时变得更不稳定。
因此一直很需要建立基本上由象木糖醇,山梨醇,麦芽糖醇,乳糖醇,蔗糖或海藻糖之类的非还原糖组成的补充能量糖源。在这些非还原糖中,象木糖醇和山梨醇之类单糖的糖醇有一个缺点,就是当向体内给药的剂量和途径不适当时会引起严重的腹泻。如日本专利公告号13,699/72和42,506/72中公开的,象麦芽糖醇和乳糖醇之类的双糖的糖醇不易被生物体代谢和利用,在实际中被用作饮食增甜剂。因此,这些双糖作为补充能量的糖源不能令人满意。蔗糖有一个缺点,就是它在酸性条件下易被水解成还原性葡萄糖和果糖,然后导致其具有差的储存稳定性。根据象日本专利公开号240,758/88中公开的描述“由于它基本上不被身体吸收和利用,海藻糖是低热量的”和“海藻糖基本上不能被淀粉酶水解等”明显看出;海藻糖一直被认为是不能作为体内能源的糖源。
建立易于工业规模制备、基本上不具有还原能力、但具有能令人满意的贮存稳定性和广泛的应用性的补充能量的糖源以及含有这种补充能量糖源作为有效成分的补充能量组合物一直是非常需要的。
发明内容
本发明人研究了易于工业规模制备的补充能量糖源。更详细地说,我们悉心地研究了一种非还原性且稳定的双糖,即海藻糖及其相关物。结果,我们发现,通过如同本发明人申请的日本专利申请号362,131/92和265,416/93中公布的新生化技术制备的海藻糖(O-α-D-吡喃葡萄糖基α-D-吡喃葡糖苷或α,α-海藻糖)易于在体内发生代谢变化并被生物体作为能源而利用。我们也发现这种生化技术是一种促进工业规模制备海藻糖的方法,该方法包括一个使非还原糖生成酶作用于具有还原能力的部分淀粉水解产物的步骤(“具有还原能力的部分淀粉水解物”一词在下文中称作“还原性部分淀粉水解产物”)。我们还发现,通过这种生化技术制备的海藻糖,由于它的非还原性而成为一种绝对新颖的、具有广泛应用性的补充能量糖源,并制得了一种包含该海藻糖作为有效成份的补充能量组合物。本发明的这种补充能量组合物由于混入其中的海藻糖具有令人满意的高贮存稳定性和基本上不具有还原能力,它可任意与其它营养品和/或药物结合应用,形成多营养组合物和具有更好治疗效果的药物组合物。
附图说明
图1表示口服海藻糖或葡萄糖后人的血糖浓度随时间的变化。
图2表示口服海藻糖或葡萄糖后人胰岛素浓度随时间的变化。
图3表示快速非肠道给入海藻糖或葡萄糖后兔血糖浓度随时间的变化。
图4表示快速非肠道给入海藻糖或葡萄糖后兔胰岛素浓度随时间的变化。
图5表示慢速非肠道给入海藻糖或葡萄糖后兔血糖浓度随时间的变化。
图6表示慢速非肠道给入海藻糖或葡萄糖后兔胰岛素浓度随时间的变化。
在所有图中,实线表示海藻糖的动力学曲线,虚线表示葡萄糖的动力学曲线。
本发明涉及一种补充能量糖源及其应用,更详细地说是涉及基本上由海藻糖组成的一种补充能量的糖源,该海藻糖是通过包含使非还原糖生成酶作用于具有还原能力的部分淀粉水解产物这一步骤在内的方法制备的,以及涉及包含该海藻糖作为有效成分的补充能量组合物。
通过包含使非还原糖生成酶作用于还原性部分淀粉水解产物这一步骤在内的方法制备的、基本上由海藻糖组成的这种补充能量糖源,和含有该海藻糖作为有效成份的这种补充能量组合物从来没有报道过。
本发明的补充能量糖源包括那些含有海藻糖最高可能浓度的组合物,并且,一般地,基于干固体它们是以海藻糖含量为50w/w%或者更高的糖浆和粉剂的形式存在(在本说明书中,若无详细说明,“w/w%”和“基于干固体”两词分别缩写为“%”和“d.s.b.”),优选的是,以糖浆和晶状粉沫形式存在的组合物中海藻糖含量为80%d.s.b.或者更高,更优选的是,以晶状粉沫和晶体形式存在的组合物中海藻糖含量为90%d.s.b.或者更高。
只要它包含使非还原糖生成酶作用于还原性部分淀粉水解产物这一步骤,任何制备海藻糖的方法都能应用于本发明中,例如,象日本专利申请号362,131/92和265,416/93中公开的制备方法,其中的海藻糖的制备是通过使非还原糖生成酶作用于葡糖聚合度为3或者更高的还原性部分淀粉水解产物,由于这些制备方法达到了海藻糖制备的工业规模,因此,它们都适合且有利地用在本发明中。
用前述制备方法制得的这种糖溶液常常含有大约20~80%d.s.b.的海藻糖,以及象葡萄糖,麦芽糖和麦芽三糖之类的还原性糖。
通过对溶液进行凝胶过滤和离心除去杂质,所得溶液用活性炭连续脱色和用H-和OH-型离子交换树脂脱盐进行纯化,并浓缩纯化后所得的溶液,用常规的方法将这种糖溶液制成糖浆制品。糖浆制品可干燥成为粉状制品。若有必要,可通过结合利用两种或多种纯化方法,而容易地制成含最可能纯海藻糖的制品,例如象离子交换柱色谱和用活性炭或硅胶的柱色谱相结合的柱色谱分级分离;用象醇或丙酮之类的有机溶剂的分级分离;用具有适当分离性能的膜分离;以及通过碱处理或用酵母发酵来分解和除去残余还原糖的其它方法。
在本发明中离子交换柱色谱尤其适用作工业规模制备最可能纯的海藻糖,例如,如同日本专利公开号23,799/83和72,598/83中公布的,通过用强酸性阳离子交换树脂在色谱柱上除去溶液中的杂质可容易地提高海藻糖原料溶液中海藻糖的含量。作为柱色谱,固定床柱色谱,流动床柱色谱和假流动方法可任意地应用。
任何补充能量组合物,只要它含有作为有效成份的海藻糖及能给生物体补充能量,且该海藻糖是通过包含使非还原糖生成酶作用于还原性部分淀粉水解物这一步骤的方法制备的,都可用于本发明中。一般来说,为达到更令人满意的效果,海藻糖以10%d.s.b.或更高,优选的是20%d.s.b.或更高的量混入这种组合物中。
该补充能量组合物包括仅含有海藻糖的组合物或海藻糖与一种或多种可食用的物质和其它可用于生物体的物质,例如,药物如蛋白质,氨基酸,类脂类,除海藻糖外的糖类,维生素,矿物质,防腐剂,酶,激素和细胞分裂素结合的组合物。若有必要,可任意混入一种或多种其它适当的物质,象调味剂,着色剂,增香剂,稳定剂,填料和赋形剂等。可将这样得到组合物制成适当的形式。
这种组合物可口服或非肠道给入生物体中,并能在体内被有益地代谢及利用作为补充能量的物质而不用担心引起毒性和副作用。
该补充能量糖源的剂量可从成人每天大约1-1000克海藻糖d.s.b.剂量范围中适当选取,优选的是成人每天大约5~500克海藻糖d.s.b.。
该补充能量糖源和含有这种糖源的组合物可有益地给入人,象牛和马之类的家畜以及象狗和猫之类供观赏动物等。
具体实施方式
下列实验详细地说明本发明:
实验1
海藻糖的制备
实验1-1
非还原糖生成酶的制备
按照日本专利申请号362,131/92中公开的方法,将培养的根瘤菌属sp.M-11(FERM BP-4130)菌株移植于含有碳源,氮源和矿物质的营养培养基中,在27℃,通风搅动的条件下培养36小时。培养完成以后,所得到的培养基用3F-膜进行膜过滤,除去细胞得到大约18升滤液,然后用UF-膜浓缩得到大约1升活性为17.7单位/毫升的非还原糖生成酶溶液。
实验1-2
含结晶水海藻糖的制备
40份重的“PINE-DEX#4”,一种被MatsutaniChemical Ind.,Co.,Ltd.,Kyoto,Japan商品化了的还原性部分淀粉水解物,通过加热溶于60份重的水中,加热所得溶液至45℃,调PH至6.5,与每克还原性部分淀粉水解物1单位的,用实验1-1的方法制得的非还原性糖生成酶混合,培养96小时,然后加热所得混合物至100℃ 10分钟使剩余的酶失活。将这样得到的溶液稀释到浓度为约20%d.s.b.,与每克还原性部分淀粉水解物10单位的“GLUCOZYME”,一种被Nagase Biochemi-cals,Ltd.,Kyoto,Japan商品化了的葡萄糖淀粉酶样品混合,并培养40小时,然后加热所得混合物使剩余酶失活。这样得到的溶液按常规方法用活性炭脱色,用离子交换树脂脱盐,并浓缩成含海藻糖29.5%d.s.b.、浓度约为60%的溶液。将此浓溶液加到充填有“CG 6000,Na-型”,一种被Japan Organo,Co.,Ltd.,Tokyo,Japan商品化了的强酸性阳离子交换树脂的不锈钢柱中,在60℃,SV(体积速度)0.4时分级,然后获得含海藻糖约90%d.s.b.的高海藻糖含量级分。浓缩此级分到约75%d.s.b.的溶液,然后转移到一结晶器中,与作为晶种的约2%的含结晶水海藻糖混合,加热到50℃,在缓缓搅动的条件下逐渐冷却到25℃,用篮式离心机分离。用少量水喷雾洗涤所得晶体,得到纯度为99%d.s.b.或更高的高纯度含结晶水海藻糖。
实验1-3
晶状海藻糖粉末的制备
通过实验1-2的方法制得结晶度约45%的糖膏,并从一装于干燥塔顶的喷嘴,以150千克/厘米2的压力喷雾干燥。在喷雾阶段,用从干燥塔的上半部分吹出的85℃的热空气吹喷出物,所得晶状粉末收集到装于干燥塔底部的一个金属网状运输装置上,同时从金属丝网运输装置的底部吹入45℃的热空气并慢慢送出干燥塔。将所得晶状粉末注入老化塔中老化10小时,同时吹入热空气以实现结晶和干燥,然后得到晶状海藻糖粉末。
实施例1-4
无结晶水海藻糖粉末的制备
将实验1-2中得到的含海藻糖糖浆制成约60%的溶液,然后按实验1-2的方法,经强酸性阳离子交换树脂柱色谱进行分离,得到含海藻糖约95%d.s.b.的高海藻糖含量级分。按照日本专利申请号265,416/93中的方法,置此级分于容器中,减压蒸水得到含水量大约4.0%的糖浆,然后转移到一结晶器中,与作为晶种的1%d.s.b.的无水晶状海藻糖混合,然后在95℃下搅拌5分钟使海藻糖结晶。将所得物转移于铝容器中,在100℃下老化6小时形成块状结晶,然后经粉碎机粉碎并经流化床干燥器干燥,得到含水量为约0.3%的无结晶水海藻糖粉末。
实验2
体外消化试验
用通过实验1-2的方法制得的高纯度含结晶水海藻糖配成的溶液,按K.okada等人在JouRNAl oF JAPANESE SOCIETYoF NUTRITION AND FOOD SCIENCE,Vol.43,NO.1,PP23-29(1990)报导的方法进行海藻糖的体外消化试验。根据按下式计算出的分解率(%)来评价海藻糖的可消化性:
结果列于表1中
表1
酶 分解率(%)
唾液淀粉酶 0.0
胃酸 0.0
胰淀粉酶 0.0
从大鼠小肠的粘膜中 1.3
提取的酶
从表1的结果中明显看出,海藻糖基本上不能被从大鼠小肠的粘膜中提取的酶水解。用类似上述的方法测定了这种大鼠酶对双糖的可消化性。
结果列于表2中。
表2
双糖 分解率(%)
麦芽糖 80.1
蔗糖 25.1
异麦芽糖 13.2
乳糖 9.7
纤维素二糖 1.2
海藻糖 1.3
从表2的结果中明显看出,海藻糖远比麦芽糖难于被此大鼠酶水解。
实验3
口服给药后的体内利用试验
按照H.Atsuji等人在Journal of Clinical Nutr-ition,Vol.41,NO.2,PP.200-208(1972)中报道的方法,将30克按实验1-2的方法制得的含结晶水海藻糖溶于水中配成20w/v%的海藻糖水溶液,年龄为26-31岁的6位男性志愿者口服了规定量的这种溶液。按规定时间间隔从志愿者体内取血,测定每一血样的血糖浓度,即葡萄糖浓度(mg/dl)和胰岛素的浓度(μu/ml)。用葡萄糖作对照。这两个浓度的结果是6位志愿者的平均值。图1和图2分别表示血糖浓度和胰岛素浓度随时间的变化。在图中,实线和虚线分别表示海藻糖和胰岛素随时间的变化曲线。
从图1和图2的结果明显看出,尽管海藻糖给兔用药后的动力学曲线与葡萄糖给兔用药相比趋于表现出时间滞后,但海藻糖给兔用药后表现出与葡萄糖给药后类似的动力学曲线,即海藻糖引起的血糖浓度和胰岛素浓度的最高峰在给药后约30-45分钟。与实验2的体内消化试验结果不同,本实验的结果表明海藻糖作为能源口服后易被人体吸收,代谢和利用。
实验4
非肠道给药后的体内利用试验
实验4-1
快速给药
将用实验1-2的方法制备的含结晶水海藻糖溶于精制水中,所得溶液经膜过滤,浓缩和重结晶得到不含热原质的含结晶水状海藻糖。将这样得到的晶体溶于注射用蒸馏水中配成10w/v%与兔血液等渗的溶液,作为海藻糖输液。用6只约2-3Kg重的兔子,将输液按每公斤体重1克的剂量在1.5分钟内快速从兔耳静脉给药,然后按规定时间间隔采集血样,测定其糖浓度(葡萄糖浓度(mg/dl))和胰岛素浓度(μu/ml)。将与兔血液等渗的5w/v%的葡萄糖溶液按每公斤体重0.5克的剂量同样从兔静脉给药作为对照。每个结果都是6只兔子的平均值,兔血糖浓度和胰岛素浓度随时间的变化曲线分别用图3和图4表示。在图中,实线和虚线分别代表兔血中海藻糖和胰岛素随时间的变化曲线。
从图3和图4的结果明显看出,使用海藻糖的兔子血糖浓度和胰岛素浓度的两条幼力学曲线的趋势是对于使用葡萄糖的曲线显示出时间滞后,即在给药后约5-30分钟出现最大值。这些结果表明当快速非肠道给药后,海藻糖在体内易于水解成葡萄糖,作为能源被生物体代谢和利用。从开始给药到给药后6小时,对兔子分泌出的尿中的糖量进行检测,结果表明与葡萄糖给药的情况相似,兔尿中分泌出的海藻糖的量绝对低,即分泌出的糖量低于海藻糖给药量的10%d.s.b.。
实验4-2
慢速给药
将用实验4-1的方法制备的海藻糖输液给6只约2-3Kg重的兔子给药,除了输液是在1.5小时期间慢慢输入到兔耳静脉以外,与实验4-1的方法相似,按规定时间间隔采集其血样,并测定这些血样中的血糖浓度(葡萄糖浓度(mg/dl))和胰岛素浓度(μm/ml)每个结果用6只兔子的平均值表示,兔血糖浓度和胰岛素浓度随时间的变化分别表示于图5和图6中。
从图5和图6的结果明显看出,尽管使用海藻糖的兔血糖浓度的动力学曲线趋势相对使用葡萄糖的情况表现出时间滞后,但实质上两条动力学曲线相同,并且使用海藻糖的兔胰岛素浓度的动力学曲线实质上与使用葡萄糖的情况相同。这些结果表明当对生物体缓慢非肠道给入海藻糖时,海藻糖在体内易于水解成葡萄糖,作为能源被生物体代谢和利用。从开始给药到给药后6小时,对兔尿中分泌出的糖量进行检测,其结果表明,与葡萄糖的情况相同,每一尿样中分泌出的海藻糖的量绝对低,即分泌出的糖量低于海藻糖给药量的10%d.s.b.。
实验5
急性毒性试验
用小鼠,通过口服按实验1-2的方法制得的水合晶状海藻糖测试海藻糖的急性毒性。结果表明海藻糖是一种毒性相当低的物质,使用最高可能的剂量给药时也无小鼠死亡。基于此结果,LD50为50g/Kg或更高,尽管这个数值并不太精确。
下列实施例说明该含有海藻糖作为有效成分补充能量组合物。
实施例1
巧克力糖
将40份重的可可酱,10份重的可可脂和50份重的通过实验1-2的方法制备的水合晶状海藻糖混合,所得混合物通过一台匀浆机来减小颗粒粒径,然后转移到一台制巧克力机中,在50℃下揉2天。在揉的过程中,向其中加入0.5份重的卵磷脂,并充分揉所得混合物。此后,通过温度调节器保持混合物在31℃,在可可脂凝固前倒入模子中,用振动器除去空气,通过10℃的冷通道时凝固20分钟。从模具上取下凝固物进行包装得到目的产品。
这种基本上不吸潮的制品有令人满意的颜色,光泽和质地,以及在口中适度熔化产生高质量的甜味和香味,这些使它任意用作补充能量的组合物。
实施例2
口香糖
加热熔化三份重的树胶基剂至软化后,与4份重的蔗糖和3份重的通过实验1-3的方法制得的晶状海藻糖粉末混合,并进一步与适量的香料、着色剂混合。按常规方法用滚筒机揉匀所得混合物并使其成型,包装得到目的产品。
具备令人满意的质地和香味的这种口香糖产品可任意地被用作补充能量组合物。
实施例3
硬糖
将100份重55%的蔗糖溶液与30份重的按实验1-4的方法制得的高海藻糖含量的溶液混合并加热,加热条件下减压浓缩所得混合物直至含水量低于2%。此浓缩物与1份重的柠檬酸和适量的柠檬香料及着色剂混合,此混合物经常规方法成型得到目的产品。
其中蔗糖的结晶作用得到很好抑制的这种产品是一种具有令人满意的味道和口感的高质量硬糖,适合于用作补充能量组合物。
实施例4
牛奶蛋糊霜
将100份重的玉米淀粉,100份重的按实验1-3制备的含有晶状海藻糖粉末70%d.s.b.的海藻糖糖浆,80份重的麦芽糖,20份重的蔗糖和1份重的盐充分揉匀,所得混合物进一步与280份重的鲜蛋边搅拌边混合,并逐渐与1000份重的沸牛奶混合。继续加热搅拌所得混合物,当全部内容物变成半透明状时停止加热,然后冷却所得物,并加入适量的香料。把产品称重,注入到容器中,包装得到目的产品。
该产品表面光滑,甜度和味道温和,这些使之任意用作补充能量组合物。
实施例5
Uiro-no-moto(Premix of uiro)
充分混合90份重的米粉与20份重的玉米淀粉,120份重按实验1-3的方法制备的结晶海藻糖粉末和4份重的粘稠性多糖,制成uiro-no-moto。该产品与适量matcha(绿茶粉)及水混揉,将此混合物装入容器中蒸60分钟得到matcha-Uiro。
该产品具有令人满意的光泽,口感及香味。该产品中淀粉的退化得到很好地抑制,并达到了令人满意的货架寿令。该产品可任意用作补充能量组合物。
实施例6
乳酸饮料
将10份重的脱脂牛奶加热至80℃消毒20分钟,冷至40℃,与0.3份重的酵母混合,在约37℃发酵10小时。此后匀化此混合物并与4份重的按实验1-3的方法制备的晶状海藻糖粉末,1份重的蔗糖,2份重的异构化的糖浆混合,加热所得混合物至70℃进行消毒。这样得到的产品经冷却,与适量的香料混合,装瓶得到目的产品。
该产品是一种香味与甜度协调得当的高质量乳酸饮料,可任意被用作补充能量组合物。
实施例7
粉汁
将33份重的经喷雾干燥的桔汁粉与50份重的用实验1-2的方法制得的水合晶状海藻糖粉末,10份重的蔗糖,0.65份重的无水柠檬酸,0.1份重的苹果酸,0.1份重的L-抗坏血酸,0.1份重的柠檬酸钠,0.5份重的粘稠性多糖和适量的香料粉搅拌混匀。将此混合物研碎,加到排气温度和流速分别调至40℃和150m3/min的流化床成粒机中,用商品海藻糖制成的高海藻糖含量的溶液作为粘合剂喷雾,粒化30分钟。称重并包装所得粉状颗粒,得到目的产品。
这种30%d.s.b.的粉汁产品能稳定相当长的时间,不会产生令人不快的味道和气味,也不会吸潮固化。因此,该产品可任意用作补充能量组合物。
实施例8
蛋黄粉
用板式加热器在60-64℃时对鲜蛋蛋黄消毒,将一份重的所得液体与4份重的用实验1-4的方法制备的无水晶状海藻糖混合。置所得混合物于容器中,放置过夜使之成块,同时使海藻糖转变成水合晶状海藻糖。用粉碎机将块状物粉碎得到蛋黄粉。
该产品可任意用作象预混物,冰淇淋和糖,之类的糖果的原料,和乳化剂,以及用于口服和插管进食的补充能量组合物。
实施例9
用于插管进食的固体制剂
制备含有下列成份的组合物:500份重的用实验1-2的方法制备的水合晶状海藻糖,270份重的蛋黄粉,209份重的脱脂牛奶,4.4份重的氯化钠,1.8份重的氯化钾,4份重的硫酸镁0.01份重的VB1,0.1份重的抗坏血酸钠,0.6份重的VE乙酸酯,0.04份重的烟酰胺。将25g此组合物的等分试样注入薄的防潮小袋中并加热密封得到目的产品。
将一袋该产品溶于约150-300ml水中配成流食,口服或通过作为补充能量组合物插管进食非肠道给到鼻腔,胃和肠中。
实施例10
高营养品
将通过实验1-2的方法制备的水合晶状海藻糖粉末溶于水中配成约10w/v%的海藻糖水溶液,然后用常规方法进行膜过滤除去热原质,无菌条件下注入塑料瓶中,密封得到目的产品。
这种稳定性良好的高营养品基本上贮存时不发生变化,适于静脉注射给药和腹膜内给药。10w/v%该产品的溶液与血液等渗,这意味着它以比葡萄糖高2倍的浓度给生物体补充能量。
实施例11
高营养品
将按实验1-2的方法制备的水合晶状海藻糖和含有下列成份的氨基酸组合物搅拌溶于水中分别配成5w/v%和30w/v%的溶液,与实施例10相似,将所得溶液纯化成不含热原质的溶液,然后注入塑料瓶中,密封得到目的产品。
氨基酸组合物中的成份
成份 mg/100ml
L-异亮氨酸 180
L-亮氨酸 410
L-赖氨酸单盐酸盐 620
L-蛋氨酸 240
L-苯丙氨酸 290
L-苏氨酸 180
L-色氨酸 60
L-缬氨酸 200
L-精氨酸盐酸盐 270
L-组氨酸单盐酸盐 130
甘氨酸 340
尽管该产品是海藻糖和氨基酸的复合高级营养品,但它实际上在贮存中没有变化,具有良好的稳定性,可适用于生物体的静脉注射和腹膜内的注射给药。该产品可很好地用作补充能量组合物以补充能量和氨基酸。
实施例12
创伤药膏
将500份重按实验1-3的方法制备的晶状海藻糖粉末与50份重含有3份重的碘的甲醇溶液混合,所得溶液与200份重10w/v%的粘稠性多糖的水溶液混合得到具有好的可扩张性和粘性的创伤药膏。
该产品中的碘具有杀菌活性,其中的海藻糖作为补充能量的糖源作用于活细胞上,因此该产品缩短了治愈期并且能很好地治愈表面创伤。
实施例13
糖衣片
150mg重的未加工药片作为核心,用含有40份重的按实验1-2的方法制得的水合晶状海藻糖,2份重的平均分子量为200,000的粘稠性多糖,30份重的水,25份重的滑石和3份重钛白的溶液进行包膜直至重约230mgd.s.b.。所得药片再用65份重的新制的同样的水合晶状海藻糖,1份重粘稠性多糖和34份重的水进行包膜。这样得到的药片用蜡溶液上光得到外观好的目的产品。
易于通过上述包膜过程制备的该产品具有相当高的耐震性,并能在相当长的时间内保持其高质量。因此适于用作补充能量组合物。
从上述明显看出,这种基本上由通过包含使非还原糖生成酶作用于还原性部分淀粉水解物这一步骤的方法制备的海藻糖构成的补充能量糖源,基本上不具有还原能力而有令人满意的稳定性,以及作为能源具有在体内易于代谢,和被生物体利用的特性。而且这种含有用新颖生化技术,由还原性部分淀粉水解物制备的作为有效成份的海藻糖的补充能量组合物,具有易于与其它营养品和药物结合制成更好的复合营养组合物和疗效高的药物组合物的特点。
在描述目前被认为是本发明较优选的实施方案的同时,各种可能进行的修改应理解为包括其中,并且这意味着在附加权利要求中包括落在本发明限定的实质和范围内的所有这些修改。
Claims (6)
1.一种生产口服施用的补充能量的组合物的方法,所述组合物包含α,α-海藻糖作为有效成分,所述方法包括下列步骤:
(a)使非还原糖生成酶作用于具有还原能力且葡萄糖聚合度为3或更高的还原性部分淀粉水解产物;
(b)使葡糖淀粉酶作用于步骤(a)中得到的混合物生成含有α,α-海藻糖的糖溶液;
(c)纯化步骤(b)中所得糖溶液以提高α,α-海藻糖纯度;
(d)回收得到的α,α-海藻糖,并向其中选择性地加入一种或多种选自食品和除所述α,α-海藻糖之外的糖的物质。
2.按照权利要求1的方法,其中在步骤(c)中包括用强酸性阳离子交换树脂对所得糖溶液进行柱色谱分离的步骤。
3.按照权利要求1的方法,其中以干固体为基准,步骤(b)中的糖溶液含20-80重量/重量%的α,α-海藻糖。
4.按照权利要求1的方法,其中以干固体为基准,所述补充能量的组合物含有至少10重量/重量%的α,α-海藻糖。
5.按照权利要求1的方法,其中所述补充能量的组合物为糖浆剂的形式。
6.一种按照权利要求1的方法获得的口服施用的补充能量的组合物。
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US5677442A (en) * | 1992-12-28 | 1997-10-14 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Method of crystallizing trehalose without using organic solvent |
ES2136115T3 (es) * | 1992-12-28 | 1999-11-16 | Hayashibara Biochem Lab | Enzima formadora de sacaridos no reductores, y su preparacion y usos. |
EP0764720B1 (en) * | 1994-06-15 | 2005-12-14 | Kirin Beer Kabushiki Kaisha | Transferase and amylase, process for producing the enzymes,use thereof, and gene coding for the same |
AU3570495A (en) * | 1994-09-22 | 1996-04-09 | Quadrant Holdings Cambridge Limited | Compositions for use in rehydration and nutrition during athletic exercise and methods of making same |
US5958455A (en) * | 1996-02-09 | 1999-09-28 | Quadrant Holdings Cambridge Ltd | Oral solid dosage forms, methods of making same and compositions thereof |
TW466116B (en) * | 1997-03-04 | 2001-12-01 | Hayashibara Biochem Lab | Reduction inhibitory agent for active-oxygen eliminating activity, method for inhibiting the reduction of said activity, and composition containing said agent |
JP4034862B2 (ja) | 1997-06-02 | 2008-01-16 | 株式会社林原生物化学研究所 | スクロースの後味改善方法とその用途 |
JPH11116588A (ja) | 1997-10-16 | 1999-04-27 | Hayashibara Biochem Lab Inc | トレハロース及び糖アルコールの製造方法 |
GB2356788A (en) * | 1999-12-02 | 2001-06-06 | British Sugar Plc | Trehalose for use in exercise |
US20030003132A1 (en) * | 2000-11-07 | 2003-01-02 | Norie Arai | Mucosal immunomodulator and use thereof |
CN103450288B (zh) * | 2013-08-16 | 2016-05-11 | 齐鲁工业大学 | 一种海藻糖的分离纯化方法 |
CN105996004A (zh) * | 2016-06-08 | 2016-10-12 | 北京蓝丹医药科技有限公司 | 一种能量补充剂 |
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JPS63240757A (ja) * | 1987-03-30 | 1988-10-06 | Natl Food Res Inst | 新規な食品素材 |
JPS63240758A (ja) * | 1987-03-30 | 1988-10-06 | Natl Food Res Inst | 新規食品素材 |
US5035912A (en) * | 1990-06-19 | 1991-07-30 | American Maize-Products Company | Starch jelly candy |
DE4022058C2 (de) * | 1990-07-11 | 1996-05-23 | Oetker Nahrungsmittel | Honigpulver und Verfahren zu seiner Herstellung |
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CA2055257C (en) * | 1991-09-20 | 2002-07-23 | Takashi Shibuya | Saccharide for supplementing energy to living body, and uses |
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CN1548049A (zh) | 2004-11-24 |
DE69431455T2 (de) | 2003-08-14 |
CA2119070A1 (en) | 1994-09-17 |
CN1188133C (zh) | 2005-02-09 |
TW422697B (en) | 2001-02-21 |
IL108965A (en) | 1998-07-15 |
AU681109B2 (en) | 1997-08-21 |
CN1101832A (zh) | 1995-04-26 |
EP0619951A3 (en) | 1995-08-02 |
DE69431455D1 (de) | 2002-11-07 |
KR940021067A (ko) | 1994-10-17 |
ATE225131T1 (de) | 2002-10-15 |
EP0619951A2 (en) | 1994-10-19 |
AU5782394A (en) | 1994-09-22 |
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