CN1243827C - 选定生物体的人工启动子文库和得自这种文库的启动子 - Google Patents

选定生物体的人工启动子文库和得自这种文库的启动子 Download PDF

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CN1243827C
CN1243827C CNB971987033A CN97198703A CN1243827C CN 1243827 C CN1243827 C CN 1243827C CN B971987033 A CNB971987033 A CN B971987033A CN 97198703 A CN97198703 A CN 97198703A CN 1243827 C CN1243827 C CN 1243827C
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promoter
sequence
seq
library
promotor
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CN1233287A (zh
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彼得·鲁戴尔·简森
卡林·哈默
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB971987033A 1996-08-23 1997-08-25 选定生物体的人工启动子文库和得自这种文库的启动子 Expired - Lifetime CN1243827C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK88696 1996-08-23
DK0886/96 1996-08-23

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CN1233287A CN1233287A (zh) 1999-10-27
CN1243827C true CN1243827C (zh) 2006-03-01

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CNB971987033A Expired - Lifetime CN1243827C (zh) 1996-08-23 1997-08-25 选定生物体的人工启动子文库和得自这种文库的启动子

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Country Link
US (1) US7199233B1 (enExample)
EP (2) EP2034021A1 (enExample)
JP (2) JP2001503249A (enExample)
CN (1) CN1243827C (enExample)
AU (1) AU3938397A (enExample)
DE (1) DE69739121D1 (enExample)
DK (1) DK0934406T3 (enExample)
WO (1) WO1998007846A1 (enExample)

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WO1999002737A1 (en) 1997-07-14 1999-01-21 Valentis, Inc. Method for the identification of synthetic cell- or tissue-specific transcriptional regulatory regions
US6475183B1 (en) 1998-06-03 2002-11-05 Baxter International Inc. Direct dual filling device for sealing agents
BRPI9909409B1 (pt) 1998-09-25 2016-03-22 Ajinomoto Kk processos para produzir um ácido l-glutâmico
US6555674B2 (en) 2000-08-09 2003-04-29 Nsgene A/S JeT promoter
US20040219525A1 (en) 2000-08-25 2004-11-04 Heiko Haertel Plant polynucleotides encoding novel prenyl proteases
EP1400593A4 (en) * 2001-06-06 2005-04-06 Takeda Pharmaceutical NEW PROMOTER
EP1497429B1 (en) 2002-04-10 2008-07-02 Novozymes A/S Improved bacillus host cell
AU2003214036A1 (en) 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
US6644365B1 (en) 2002-04-19 2003-11-11 Baxter International, Inc. Tilting direct dual filling device
EP1499709B1 (en) * 2002-04-22 2012-01-04 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
WO2003089604A2 (en) * 2002-04-22 2003-10-30 Genencor International, Inc. Methods of creating modified promoters resulting in varying levels of gene expression
US20080076678A1 (en) 2005-06-15 2008-03-27 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
ATE401422T1 (de) * 2002-04-22 2008-08-15 Genencor Int Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus
EP2428573A3 (en) 2002-10-04 2012-06-27 Genecor International, Inc. Improved production of bacterial strains cross reference to related applications
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
EP1579005A4 (en) * 2002-11-15 2007-07-25 Sangamo Biosciences Inc METHOD AND COMPOSITIONS FOR THE ANALYSIS OF REGULATORY SEQUENCES
JP4821606B2 (ja) 2003-03-12 2011-11-24 味の素株式会社 効率的にＬ−グルタミン酸を生産するためのプロモーター変異による細菌のｓｕｃＡＢ発現の最適化
WO2006116400A2 (en) 2005-04-27 2006-11-02 Massachusetts Institute Of Technology Promoter engineering and genetic control
WO2007146975A2 (en) * 2006-06-13 2007-12-21 Athenix Corporation Methods for generating genetic diversity by permutational mutagenesis
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
CN101600800B (zh) 2006-11-29 2013-08-28 埃塞尼克斯公司 改良的grg23 epsp合酶：组合物和使用方法
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
CN101981193B (zh) 2008-03-28 2016-05-11 丹尼斯科美国公司 在细菌细胞中扩增基因座的方法
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2311961A1 (en) * 2009-10-15 2011-04-20 DKFZ Deutsches Krebsforschungszentrum Model-guided random assembly PCR for the synthesis of eukaryotic promoters
US20120315670A1 (en) 2009-11-02 2012-12-13 Gen9, Inc. Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
EP4039363A1 (en) 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
CN102286517B (zh) * 2011-06-10 2016-08-03 中国科学院天津工业生物技术研究所 利用人工调控元件及其文库调控微生物染色体上基因表达强度的方法
IL302248A (en) 2011-08-26 2023-06-01 Gen9 Inc Compositions and methods for high fidelity assembly of nucleic acids
EP2573172A1 (en) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Means and methods for rhamnolipid production
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
EP3483311B8 (en) 2012-06-25 2025-10-29 Ginkgo Bioworks, Inc. Methods for nucleic acid assembly and high throughput sequencing
WO2014159850A2 (en) 2013-03-14 2014-10-02 Codexis, Inc. Low-phosphate repressible promoter
US10907180B2 (en) 2015-07-08 2021-02-02 Rheinisch-Westfaelische Technische Hochshule Aachen (RWTH) Extracellular production of designer hydroxyalkanoyloxy alkanoic acids with recombinant bacteria
CN105296486B (zh) * 2015-11-18 2019-04-26 湖北大学 一种启动子文库构建方法和强启动子
US9988624B2 (en) * 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) * 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
CN105603537A (zh) * 2016-03-11 2016-05-25 南京工业大学 一种启动子文库的构建方法及其应用
CA3064777A1 (en) * 2017-06-07 2018-12-13 Zach Serber Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
EP3546582A1 (en) * 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
AU2019375413A1 (en) * 2018-11-05 2021-05-27 Genentech, Inc. Methods of producing two chain proteins in prokaryotic host cells
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统
CN115175994A (zh) * 2019-12-16 2022-10-11 银杏生物制品公司 组氨酸、嘌呤途径代谢物和质粒dna的增强的产生

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GB9215550D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
WO1994025609A1 (en) * 1993-04-28 1994-11-10 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking

Also Published As

Publication number Publication date
EP0934406A2 (en) 1999-08-11
JP4469005B2 (ja) 2010-05-26
JP2001503249A (ja) 2001-03-13
EP0934406B1 (en) 2008-11-19
DK0934406T3 (da) 2009-01-26
DE69739121D1 (de) 2009-01-02
JP2009022293A (ja) 2009-02-05
CN1233287A (zh) 1999-10-27
US7199233B1 (en) 2007-04-03
EP2034021A1 (en) 2009-03-11
AU3938397A (en) 1998-03-06
WO1998007846A1 (en) 1998-02-26

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