CN1243827C - 选定生物体的人工启动子文库和得自这种文库的启动子 - Google Patents

选定生物体的人工启动子文库和得自这种文库的启动子 Download PDF

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CN1243827C
CN1243827C CNB971987033A CN97198703A CN1243827C CN 1243827 C CN1243827 C CN 1243827C CN B971987033 A CNB971987033 A CN B971987033A CN 97198703 A CN97198703 A CN 97198703A CN 1243827 C CN1243827 C CN 1243827C
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promoter
sequence
seq
library
promotor
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CN1233287A (zh
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彼得·鲁戴尔·简森
卡林·哈默
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
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  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB971987033A 1996-08-23 1997-08-25 选定生物体的人工启动子文库和得自这种文库的启动子 Expired - Lifetime CN1243827C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK88696 1996-08-23
DK0886/96 1996-08-23

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CN1233287A CN1233287A (zh) 1999-10-27
CN1243827C true CN1243827C (zh) 2006-03-01

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CNB971987033A Expired - Lifetime CN1243827C (zh) 1996-08-23 1997-08-25 选定生物体的人工启动子文库和得自这种文库的启动子

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Country Link
US (1) US7199233B1 (enExample)
EP (2) EP2034021A1 (enExample)
JP (2) JP2001503249A (enExample)
CN (1) CN1243827C (enExample)
AU (1) AU3938397A (enExample)
DE (1) DE69739121D1 (enExample)
DK (1) DK0934406T3 (enExample)
WO (1) WO1998007846A1 (enExample)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999002737A1 (en) 1997-07-14 1999-01-21 Valentis, Inc. Method for the identification of synthetic cell- or tissue-specific transcriptional regulatory regions
US6475183B1 (en) 1998-06-03 2002-11-05 Baxter International Inc. Direct dual filling device for sealing agents
CN1170938C (zh) 1998-09-25 2004-10-13 味之素株式会社 构建产生氨基酸的细菌的方法及通过发酵该经构建的产生氨基酸的细菌以制备氨基酸的方法
US6555674B2 (en) 2000-08-09 2003-04-29 Nsgene A/S JeT promoter
EP2157183A1 (en) 2000-08-25 2010-02-24 BASF Plant Science GmbH Plant polynucleotides encoding prenyl proteases
US20040166566A1 (en) * 2001-06-06 2004-08-26 Takashi Ito Novel promoter
US7521204B2 (en) 2002-04-10 2009-04-21 Novozymes A/S Bacillus host cell
AU2003214036A1 (en) 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
US6644365B1 (en) 2002-04-19 2003-11-11 Baxter International, Inc. Tilting direct dual filling device
US20080076678A1 (en) 2005-06-15 2008-03-27 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
CA2481414C (en) * 2002-04-22 2013-04-09 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
EP1576094B1 (en) * 2002-04-22 2011-09-28 Danisco US Inc. Methods of creating modified promoters resulting in varying levels of gene expression
WO2003089605A2 (en) * 2002-04-22 2003-10-30 Genencor International, Inc. Method of creating a library of bacterial clones with varying levels of gene expression
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
EP2428573A3 (en) 2002-10-04 2012-06-27 Genecor International, Inc. Improved production of bacterial strains cross reference to related applications
AU2003295692A1 (en) * 2002-11-15 2004-06-15 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
BRPI0407966A (pt) 2003-03-12 2006-03-07 Ajinomoto Kk método para obter uma bactéria, bactéria produzindo l-aminoácido, e, método para produzir um l-aminoácido
WO2006116400A2 (en) 2005-04-27 2006-11-02 Massachusetts Institute Of Technology Promoter engineering and genetic control
WO2007146975A2 (en) * 2006-06-13 2007-12-21 Athenix Corporation Methods for generating genetic diversity by permutational mutagenesis
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
BRPI0719672A2 (pt) 2006-11-29 2013-12-24 Athenix Corp Molécula isolada de ácido nucleico, vetor, célula hospedeira, planta transgênica, semente transgênica, polipeptídeo isolado, método para produzir um polipeptídeo com atividade de resistência a herbicidas, método para conferir resistência a um herbicida em uma planta, planta, método para aumentar o vigor ou rendimento de uma planta, método para conferir resistência ao glifosato a uma planta
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
KR101683302B1 (ko) 2008-03-28 2016-12-06 다니스코 유에스 인크. 박테리아 세포 내 로커스 증폭 방법
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2311961A1 (en) * 2009-10-15 2011-04-20 DKFZ Deutsches Krebsforschungszentrum Model-guided random assembly PCR for the synthesis of eukaryotic promoters
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
AU2011338841B2 (en) 2010-11-12 2017-02-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
CN102286517B (zh) * 2011-06-10 2016-08-03 中国科学院天津工业生物技术研究所 利用人工调控元件及其文库调控微生物染色体上基因表达强度的方法
AU2012300401B2 (en) 2011-08-26 2018-02-08 Ginkgo Bioworks, Inc. Compositions and methods for high fidelity assembly of nucleic acids
EP2573172A1 (en) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Means and methods for rhamnolipid production
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2841601B1 (en) 2012-04-24 2019-03-06 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20150191719A1 (en) 2012-06-25 2015-07-09 Gen9, Inc. Methods for Nucleic Acid Assembly and High Throughput Sequencing
US9670493B2 (en) 2013-03-14 2017-06-06 Codexis, Inc. Low-phosphate repressible promoter
BR112018000257A2 (pt) 2015-07-08 2018-09-04 Rheinisch Westfaelische Technische Hochschule Aachen Rwth célula hospedeira, método para produzir um haa, uso de uma célula hospedeira, método para produzir uma célula hospedeira, célula, preparação de haa, método para produzir uma composição de ácido graxo e composição de ácido graxo
CN105296486B (zh) * 2015-11-18 2019-04-26 湖北大学 一种启动子文库构建方法和强启动子
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) * 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CN105603537A (zh) * 2016-03-11 2016-05-25 南京工业大学 一种启动子文库的构建方法及其应用
CA3064777A1 (en) * 2017-06-07 2018-12-13 Zach Serber Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
EP3546582A1 (en) * 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
KR20210090645A (ko) 2018-11-05 2021-07-20 제넨테크, 인크. 원핵 숙주 세포에서 2개의 사슬 단백질을 생산하는 방법
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统
AU2020405305A1 (en) * 2019-12-16 2022-07-14 Ginkgo Bioworks, Inc. Enhanced production of histidine, purine pathway metabolites, and plasmid DNA

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215550D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
WO1994025609A1 (en) * 1993-04-28 1994-11-10 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking

Also Published As

Publication number Publication date
US7199233B1 (en) 2007-04-03
CN1233287A (zh) 1999-10-27
EP0934406A2 (en) 1999-08-11
AU3938397A (en) 1998-03-06
JP2001503249A (ja) 2001-03-13
DK0934406T3 (da) 2009-01-26
WO1998007846A1 (en) 1998-02-26
JP4469005B2 (ja) 2010-05-26
DE69739121D1 (de) 2009-01-02
EP2034021A1 (en) 2009-03-11
EP0934406B1 (en) 2008-11-19
JP2009022293A (ja) 2009-02-05

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