ATE401422T1 - Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus - Google Patents

Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus

Info

Publication number
ATE401422T1
ATE401422T1 AT03724100T AT03724100T ATE401422T1 AT E401422 T1 ATE401422 T1 AT E401422T1 AT 03724100 T AT03724100 T AT 03724100T AT 03724100 T AT03724100 T AT 03724100T AT E401422 T1 ATE401422 T1 AT E401422T1
Authority
AT
Austria
Prior art keywords
library
expression levels
generating
gene expression
bacterial clones
Prior art date
Application number
AT03724100T
Other languages
English (en)
Inventor
Philippe Soucaille
Marguerite A Cervin
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE401422T1 publication Critical patent/ATE401422T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03724100T 2002-04-22 2003-04-18 Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus ATE401422T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37462702P 2002-04-22 2002-04-22

Publications (1)

Publication Number Publication Date
ATE401422T1 true ATE401422T1 (de) 2008-08-15

Family

ID=29251213

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724100T ATE401422T1 (de) 2002-04-22 2003-04-18 Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus

Country Status (9)

Country Link
US (1) US20060014146A1 (de)
EP (1) EP1501947B1 (de)
JP (1) JP2005523015A (de)
AT (1) ATE401422T1 (de)
AU (1) AU2003230982A1 (de)
CA (1) CA2483338C (de)
DE (1) DE60322210D1 (de)
DK (1) DK1501947T3 (de)
WO (1) WO2003089605A2 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7252942B2 (en) * 2002-12-19 2007-08-07 E.I. Du Pont De Nemours And Company Parallel chromosomal stacking of traits in bacteria
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
AU2008252990B2 (en) * 2007-05-17 2015-01-15 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
RU2396336C2 (ru) 2007-09-27 2010-08-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia
EP2227553A4 (de) * 2007-11-30 2011-01-19 Scarab Genomics Llc Lac-expressionssystem
AU2009266989B2 (en) * 2008-07-03 2013-05-02 Pfenex, Inc. High throughput screening method and use thereof to identify a production platform for a multifunctional binding protein
US20120315670A1 (en) 2009-11-02 2012-12-13 Gen9, Inc. Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2748318B1 (de) 2011-08-26 2015-11-04 Gen9, Inc. Zusammensetzungen und verfahren für hochqualitative anordnung von nukleinsäuren
EP2573172A1 (de) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Mittel und Verfahren zur Rhamnolipidherstellung
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
EP4610368B1 (de) 2013-08-05 2026-02-04 Twist Bioscience Corporation De-novo-synthetisierte genbibliotheken
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA3253836A1 (en) 2015-02-04 2025-12-01 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
BR112018000257A2 (pt) 2015-07-08 2018-09-04 Rheinisch Westfaelische Technische Hochschule Aachen Rwth célula hospedeira, método para produzir um haa, uso de uma célula hospedeira, método para produzir uma célula hospedeira, célula, preparação de haa, método para produzir uma composição de ácido graxo e composição de ácido graxo
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods for modulating protein and cellular activity and method for nucleic acid synthesis
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP4556433A3 (de) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nukleinsäurebasierte datenspeicherung
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN111566209B (zh) 2017-06-12 2024-08-30 特韦斯特生物科学公司 无缝核酸装配方法
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3681906A4 (de) 2017-09-11 2021-06-09 Twist Bioscience Corporation Gpcr-bindende proteine und deren synthese
KR102889470B1 (ko) 2017-10-20 2025-11-21 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
WO2019123324A1 (en) * 2017-12-21 2019-06-27 Glycom A/S Nucleic acid construct for in vitro and in vivo gene expression
CA3088911A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based storage device and method for synthesizing polynucleotides using the device
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
WO2019222706A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１受容体の変異体核酸ライブラリ
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
NL2033707B1 (en) * 2022-12-09 2024-06-14 Stichting Sanquin Bloedvoorziening Altering RNA sequences in non-coding regions for modification of protein expression
WO2024263832A2 (en) * 2023-06-21 2024-12-26 The Texas A&M University System Click display compositions and methods

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
WO1994025609A1 (en) * 1993-04-28 1994-11-10 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking
NZ312332A (en) * 1995-06-07 2000-01-28 Life Technologies Inc Recombinational cloning using engineered recombination sites
DE69739121D1 (de) * 1996-08-23 2009-01-02 Peter Ruhdal Jensen Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination

Also Published As

Publication number Publication date
US20060014146A1 (en) 2006-01-19
DE60322210D1 (de) 2008-08-28
AU2003230982A1 (en) 2003-11-03
WO2003089605A2 (en) 2003-10-30
JP2005523015A (ja) 2005-08-04
EP1501947A4 (de) 2006-04-26
CA2483338C (en) 2014-10-14
AU2003230982A8 (en) 2003-11-03
EP1501947B1 (de) 2008-07-16
DK1501947T3 (da) 2008-11-17
WO2003089605A3 (en) 2004-12-02
EP1501947A2 (de) 2005-02-02
CA2483338A1 (en) 2003-10-30

Similar Documents

Publication Publication Date Title
ATE401422T1 (de) Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus
ATE526419T1 (de) Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren
DE50303528D1 (de) Verfahren zur Erzeugung natürlicher Sprache in Computer-Dialogsystemen
DE60239551D1 (de) Verfahren zur Erzeugung von 3D-Ausdrucken
DE60237184D1 (de) Verfahren zur Erzeugung von Bewegungen
ATE513898T1 (de) Verfahren zur verwendung von probiotischen bifidobakterien für haustiere
DE602004018971D1 (de) Verfahren zur gewinnung von polyhydroxyalkanolaten ( phas ) aus zellbiomasse
WO2003060084A3 (en) Methods for making polynucleotides and purifying double-stranded polynucleotides
DE60236861D1 (de) Kombinatorische bibliotheken von monomerdomänen
ATE339484T1 (de) Verfahren zur herstellung von fischer-tropsch- wachsen
DE602004026698D1 (de) Verfahren zur gemeinsmen benutzung von multimedia-daten
ATE342990T1 (de) Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus
CY1106821T1 (el) Μεθοδος για την καλλιεργεια υποκαταστατου χονδρου και βιολογικη μητρα, παραγομενη συμφωνα με τη μεθοδο αυτη
DE60323067D1 (de) Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
DE60128270D1 (de) Verfahren und System zur Erzeugung von Sprechererkennungsdaten, und Verfahren und System zur Sprechererkennung
DE60216756D1 (de) Verfahren zur herstellung von hochdichten produkten aus rostfreiem stahl
BRPI0917352A2 (pt) Métodos para produção de uma biblioteca de pares cognatos que compreende sequências ligadas que codificam regiões variáveis e para gerar um vetor que codifica um anticorpo quimérico com regiões de seres humanos constantes e regiões variáveis não humanas, biblioteca de vetores, e método para produção de uma biblioteca de sequências de imunoglobulina derivadas de aves
ATE437906T1 (de) Verfahren zur herstellung von pfropfpolymerisaten
DE60327607D1 (de) Verfahren zur herstellung von zusammensetzungen auf basis von expandierbaren styrolpolymerisaten mit verbesserter expandierbarkeit
ATE442459T1 (de) MIKROORGANISMUS MIT EINEM ßKNOCK-OUTß FADR GEN UND DESSEN VERWENDUNG IN EINEM VERFAHREN ZUR HERSTELLUNG VON L-THREONIN
ATE444949T1 (de) Verfahren zur herstellung von n-monoalkyl-beta- aminoalkoholen
ATE458047T1 (de) Verfahren zur verstärkung der oligonucleotid- vermittelten nucleinsäuresequenzänderung unter verwendung von zusammensetzungen mit einem hydroxyharnstoff
ATE408029T1 (de) Verfahren zur herstellung und identifizierung löslicher proteindomänen
WO2005026962A3 (en) Improvements in or relating to test systems or programs
ATE505535T1 (de) Verfahren zur herstellung stabiler b-lymphozyten

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties