ATE342990T1 - Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus - Google Patents

Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus

Info

Publication number
ATE342990T1
ATE342990T1 AT02755966T AT02755966T ATE342990T1 AT E342990 T1 ATE342990 T1 AT E342990T1 AT 02755966 T AT02755966 T AT 02755966T AT 02755966 T AT02755966 T AT 02755966T AT E342990 T1 ATE342990 T1 AT E342990T1
Authority
AT
Austria
Prior art keywords
vector
pgsbca
gamma
target protein
poly
Prior art date
Application number
AT02755966T
Other languages
English (en)
Inventor
Moon-Hee Sung
Seung-Pyo Hong
Jong-Su Lee
Chang-Min Jung
Chul-Joong Kim
Kenji Soda
Makoto Ashiuchi
Original Assignee
Bioleaders Corp
M D Lab Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioleaders Corp, M D Lab Co Ltd filed Critical Bioleaders Corp
Application granted granted Critical
Publication of ATE342990T1 publication Critical patent/ATE342990T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01133—Xylosylprotein 4-beta-galactosyltransferase (2.4.1.133)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08005—CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase (2.7.8.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT02755966T 2001-08-10 2002-08-09 Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus ATE342990T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20010048373 2001-08-10

Publications (1)

Publication Number Publication Date
ATE342990T1 true ATE342990T1 (de) 2006-11-15

Family

ID=19713096

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02755966T ATE342990T1 (de) 2001-08-10 2002-08-09 Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus

Country Status (8)

Country Link
US (1) US7553636B2 (de)
EP (1) EP1421195B1 (de)
JP (1) JP4458844B2 (de)
KR (1) KR100469800B1 (de)
CN (1) CN1311082C (de)
AT (1) ATE342990T1 (de)
DE (1) DE60215521T2 (de)
WO (1) WO2003014360A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4549858B2 (ja) 2002-10-17 2010-09-22 バイオリーダーズ コーポレイション ヒト・パピローマウイルスに対するワクチン用ベクターおよび同ベクターによって形質転換された微生物
KR100525759B1 (ko) * 2002-10-17 2005-11-03 한국생명공학연구원 바실러스 속 균주 유래의 폴리 감마 글루탐산합성유전자를 이용한 펩타이드 항생물질 Ｐ5와Ａｎａｌ3의 표면 발현 방법 및 그의 용도
AU2004245859B2 (en) * 2003-06-04 2007-02-08 Bioleaders Corporation Cell surface expression vector of SARS virus antigen and microorganisms transformed thereby
KR20050079162A (ko) * 2004-02-04 2005-08-09 주식회사 바이오리더스 파보바이러스 항원의 세포표면 발현벡터 및 상기 벡터에의해 형질전환된 미생물
US7255855B2 (en) 2004-02-27 2007-08-14 Bioleaders Corporation Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase
JP2005312426A (ja) * 2004-03-31 2005-11-10 Bioleaders Japan:Kk タンパク質を表層提示する大腸菌
EP1854348A4 (de) * 2005-03-04 2009-02-25 Suntory Ltd Transformierte pflanze, die zur produktion von polyglutaminsäure befähigt ist
JP2006262724A (ja) * 2005-03-22 2006-10-05 Kobe Univ タンパク質高発現乳酸菌
JPWO2006126682A1 (ja) * 2005-05-27 2008-12-25 財団法人化学及血清療法研究所 アルツハイマー病の予防・治療用ワクチン
KR100872042B1 (ko) * 2005-09-14 2008-12-05 주식회사 바이오리더스 마이오스타틴을 발현하는 세포표면 발현벡터 및 상기벡터에 의해 형질전환된 미생물
KR100578395B1 (ko) * 2005-11-11 2006-05-10 주식회사 바이오리더스 면역기능이 강화된 사균화 유산균 제제 및 그 제조방법
KR100782332B1 (ko) * 2006-01-23 2007-12-06 주식회사 바이오리더스 새우 흰 반점 바이러스 항원의 표면 발현벡터 및 이에 의해형질전환된 미생물
KR100985914B1 (ko) * 2006-12-13 2010-10-08 주식회사 바이오리더스 간암 특이 항원인 알파­페토단백질의 표면발현벡터 및 이에의해 형질전환된 미생물
KR100972843B1 (ko) * 2007-03-22 2010-07-28 주식회사 바이오리더스 신규 항시적 고발현 프로모터 및 그 용도
KR100857861B1 (ko) 2007-10-15 2008-09-11 주식회사 바이오리더스 Ｍｙｏ－２ 펩타이드 중합체와 마이오스타틴의 융합단백질표면발현용 벡터 및 상기 벡터로 형질전환된 미생물
KR101564084B1 (ko) * 2009-01-08 2015-10-29 주식회사 바이오리더스 ｒｅｐＥ 변이유전자를 함유하는 항시적 고발현 벡터
KR101471043B1 (ko) 2009-01-08 2014-12-09 주식회사 바이오리더스 안정적인 항시적 고발현 자궁경부암 치료백신용 벡터 및 그에 의해 형질전환된 재조합 유산균
JP5669361B2 (ja) * 2009-03-17 2015-02-12 花王株式会社 ポリ−ガンマ−グルタミン酸の調整方法
JP5599153B2 (ja) * 2009-03-17 2014-10-01 花王株式会社 ポリ−ガンマ−グルタミン酸の製造方法
JP5561681B2 (ja) 2009-09-17 2014-07-30 森下仁丹株式会社 ビフィズス菌表層提示融合タンパク質発現遺伝子
JP5846523B2 (ja) * 2010-11-18 2016-01-20 国立大学法人神戸大学 βグルコシダーゼを発現する大腸菌
JP2014210747A (ja) 2013-04-19 2014-11-13 アンジェスＭｇ株式会社 細胞性免疫誘導能が改善された経口ワクチン
CN103555720B (zh) * 2013-11-19 2015-08-19 湖北工业大学 一种具有生产和回收纤维素内切酶双重功能的酵母菌株的构建
US10695385B2 (en) 2015-06-25 2020-06-30 National University Corporation Kobe University Oral cancer vaccine
US11666646B2 (en) 2016-12-26 2023-06-06 National University Corporation Kobe University Cancer therapy utilizing combination of oral tumor vaccine and immunosuppression inhibitor
KR20200000972A (ko) * 2018-06-26 2020-01-06 주식회사 바이오리더스 바실러스 속 균주 유래의 폴리감마글루탐산 합성유전자를 이용한 표면발현벡터 및 이를 이용한 단백질의 미생물표면 발현 방법
KR102594583B1 (ko) 2018-10-10 2023-10-27 주식회사 비엘 락토바실러스 카제이 유래 갈락토오스 뮤타로테이즈 유전자의 프로모터를 이용한 항시적 고발현 표면발현벡터 및 그 이용
EP3940077A4 (de) * 2018-10-10 2022-08-10 Bioleaders Corporation Oberflächenexpressionsvektor mit zwei arten von promotoren aus lactobacillus casei zur gleichzeitigen expression zweier zielproteine und verfahren zur expression von proteinen auf einer mikrobiellen oberfläche unter verwendung desselben
CN111593029B (zh) * 2020-05-29 2022-05-17 江西省科学院微生物研究所 一种催化活性提高的γ-聚谷氨酸合成酶复合体及其编码基因
CN114702559A (zh) * 2022-04-02 2022-07-05 江西师范大学 枯草芽孢杆菌蛋白PgsA及其在表面展示系统中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5348867A (en) 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
US5356797A (en) 1991-11-15 1994-10-18 Board Of Regents, The University Of Texas Membrane expression of heterologous genes
WO1993024636A1 (en) 1992-05-29 1993-12-09 The University Of British Columbia Use of protein oprf for bacterial cell surface expression of oligopeptides
WO1994010330A1 (en) 1992-11-03 1994-05-11 Rea, Teresa, Stanek Fibronectin binding protein as well as its preparation
DE4243770A1 (de) * 1992-12-23 1994-06-30 Behringwerke Ag Verfahren zur Herstellung von rekombinanten und synthetischen Peptiden und deren Verwendung
EP0711303B2 (de) 1993-07-30 2009-06-10 Affymax, Inc. Biotinylierung von proteinen
FR2708622B1 (fr) 1993-08-02 1997-04-18 Raymond Hamers Vecteur recombinant contenant une séquence d'un gène de lipoprotéine de structure pour l'expression de séquences de nucléotides.
KR0185335B1 (ko) * 1996-04-02 1999-04-01 김은영 표면 발현 벡터 및 이를 이용한 단백질의 미생물 표면 발현 방법
JP2001017182A (ja) * 1999-07-09 2001-01-23 Nagase & Co Ltd ポリ−γ−グルタミン酸の製造方法

Also Published As

Publication number Publication date
JP2005500054A (ja) 2005-01-06
US20040253704A1 (en) 2004-12-16
KR20040032824A (ko) 2004-04-17
EP1421195A1 (de) 2004-05-26
DE60215521T2 (de) 2007-06-21
JP4458844B2 (ja) 2010-04-28
CN1311082C (zh) 2007-04-18
EP1421195B1 (de) 2006-10-18
CN1539018A (zh) 2004-10-20
EP1421195A4 (de) 2005-04-27
DE60215521D1 (de) 2006-11-30
KR100469800B1 (ko) 2005-02-02
WO2003014360A1 (en) 2003-02-20
US7553636B2 (en) 2009-06-30

Similar Documents

Publication Publication Date Title
ATE342990T1 (de) Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus
WO2002083869A3 (en) Product and process for transformation of thraustochytriales microorganisms
BR0015114A (pt) Sequências de ácido nucléico e de aminoácido, construção de ácido nucleico, vetor, microorganismo, l-pantolactona hidrolase, e, processo para preparar d-pantolactona
ATE536415T1 (de) Klonierung des cytochrome p450 gens von nicotiana
MXPA05004016A (es) Clonacion de genes de citocromo p450 de nicotiana.
ATE513910T1 (de) Methoden zur gensuppression mithilfe von rnai verstärkenden faktoren
ATE550430T1 (de) Aus bakterien der gattung corynebacterium stammende promoternukleinsäure , den promoter umfassende expressionskassette , den vektor umfassende wirtszelle sowie verfahren zur expression eines gens unter verwendung der zelle .
EP2395092A3 (de) Pflanzen mit verbesserten Ertragseigenschaften und Verfahren zu deren Herstellung
ATE474847T1 (de) Verfahren zur herstellung von dipeptiden
EP2444810A3 (de) Mutanten von Proteasen mit veränderter Spezifizität und ihre Verwendung
MX2011011454A (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas.
ATE471381T1 (de) Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektors
ATE355382T1 (de) Verfahren zur fermentativen herstellung von l- threonine
DE50013609D1 (de) Elektronendonorsystem für enzyme und dessen anwendung bei der biochemischen umsetzung von substraten
AU8491401A (en) Phage-dependent superproduction of biologically active protein and peptides
ATE485381T1 (de) Verfahren zur einführung eines gens in pflanzenmaterial
ATE451457T1 (de) Verfahren zur dipeptidherstellung
WO2001072977A3 (en) Isolated human drug-metabolizing proteins, nucleic acid molecules encoding human drug-metabolizing proteins, and uses thereof
DK1368480T3 (da) Fremgangsmåde til ændring af genomet i grampositive bakterier ved hjælp af et nyt konditionalt negativt dominant markörgen
ATE553194T1 (de) Verfahren zur dipeptidherstellung
WO2002034922A3 (en) Isolated human drug-metabolizing proteins, nucleic acid molecules encoding human drug-metabolizing proteins, and uses thereof
WO2002042437A3 (en) Isolated human drug-metabolizing proteins, nucleic acid molecules encoding human drug-metabolizing proteins, and uses thereof
WO2001083754A3 (en) Cellular regulators of infectious agents and methods of use
AU2003247599A1 (en) Methods of modulating cytokinin related processes in a plant using b3 domain proteins
PT1507009E (pt) Vector para o silenciamento do gene e processo do silenciamento do gene utilizando o mesmo

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties