ATE536415T1 - Klonierung des cytochrome p450 gens von nicotiana - Google Patents

Klonierung des cytochrome p450 gens von nicotiana

Info

Publication number
ATE536415T1
ATE536415T1 AT04795252T AT04795252T ATE536415T1 AT E536415 T1 ATE536415 T1 AT E536415T1 AT 04795252 T AT04795252 T AT 04795252T AT 04795252 T AT04795252 T AT 04795252T AT E536415 T1 ATE536415 T1 AT E536415T1
Authority
AT
Austria
Prior art keywords
nicotiana
cytochrome
cloning
gene
enzymes
Prior art date
Application number
AT04795252T
Other languages
English (en)
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/686,947 external-priority patent/US20040162420A1/en
Priority claimed from US10/934,944 external-priority patent/US7812227B2/en
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Application granted granted Critical
Publication of ATE536415T1 publication Critical patent/ATE536415T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT04795252T 2003-10-16 2004-10-15 Klonierung des cytochrome p450 gens von nicotiana ATE536415T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana
US56623504P 2004-04-29 2004-04-29
US10/934,944 US7812227B2 (en) 2001-11-13 2004-09-03 Cloning of cytochrome p450 genes from nicotiana
PCT/US2004/034065 WO2005038033A2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
ATE536415T1 true ATE536415T1 (de) 2011-12-15

Family

ID=34468385

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04795252T ATE536415T1 (de) 2003-10-16 2004-10-15 Klonierung des cytochrome p450 gens von nicotiana

Country Status (9)

Country Link
EP (1) EP1675957B1 (de)
JP (2) JP5422099B2 (de)
CN (1) CN102321641A (de)
AT (1) ATE536415T1 (de)
AU (3) AU2004282576B2 (de)
BR (1) BRPI0415363A (de)
ES (1) ES2377868T3 (de)
PL (1) PL1675957T3 (de)
WO (1) WO2005038033A2 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
BRPI0415362A (pt) * 2003-10-16 2006-12-12 Us Smokeless Tobacco Co molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta
EP2330191A1 (de) 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Klonen von Cytochrom P450-Genen aus Nikotiana
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US9370160B2 (en) 2006-12-15 2016-06-21 Altria Client Services Llc Tobacco inbred plants ALBEX1F and ALBEX1MS
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8124851B2 (en) 2007-11-12 2012-02-28 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
MX337123B (es) 2010-01-15 2016-02-12 Univ North Carolina State Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco.
US9096864B2 (en) 2011-02-28 2015-08-04 North Carolina State University Tobacco inbred plants NCBEX1F, NCBEX1MS, and NC EX90
CN103361368B (zh) * 2012-03-27 2015-03-04 华中农业大学 一种棉花细胞色素p450基因及应用
CN102796821A (zh) * 2012-08-30 2012-11-28 浙江中烟工业有限责任公司 一种检测烟草烟碱转化相对水平的方法
EP2943059A1 (de) 2013-01-11 2015-11-18 North Carolina State University Tabakinzuchtpflanzen k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src und hybrid-nc196 src
US9560830B2 (en) 2013-03-05 2017-02-07 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
WO2015051454A1 (en) * 2013-10-07 2015-04-16 Vineland Research And Innovation Centre Compositions and methods for producing steviol and steviol glycosides
EP3113604A1 (de) 2014-03-03 2017-01-11 North Carolina State University Inzucht- und hybridtabakpflanzen und daraus hergestellte tabakprodukte
EP3113603A1 (de) 2014-03-03 2017-01-11 North Carolina State University Inzucht- und hybridtabakpflanzen und daraus hergestellte tabakprodukte
WO2015134423A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CN110305893B (zh) * 2018-03-23 2022-09-06 中国科学院分子植物科学卓越创新中心 棉酚生物合成途径基因cyp71be79及其应用
CN111849931A (zh) * 2019-04-26 2020-10-30 西南大学 川桑色氨酸羟化酶t5h2及其应用
CN110923249A (zh) * 2019-10-15 2020-03-27 贵州省烟草科学研究院 烟草CyP71及在调控植物表皮毛发育方面的应用
JP2023504511A (ja) * 2019-12-03 2023-02-03 アルトリア クライアント サーヴィシーズ リミテッド ライアビリティ カンパニー 変化したアルカロイドレベルを有するタバコ植物およびタバコ製品を作製するための組成物および方法
CN110885841B (zh) * 2019-12-24 2022-04-12 河南科技大学 葡萄VyCYP89A2基因及其编码蛋白和基因在抗旱品种育种中的应用
CN113234739B (zh) * 2021-05-26 2023-12-26 云南中烟工业有限责任公司 烟草细胞色素p450亚家族cyp710a基因及应用
CN118272399A (zh) * 2024-04-19 2024-07-02 华中农业大学 一种应用OfCYP244基因提高桂花花瓣香气物质芳樟醇的方法
CN118773150B (zh) * 2024-06-03 2025-06-17 上海大学 一种银胶菊素c2羟化酶及其编码基因、制备方法与应用
CN119876057B (zh) * 2025-01-16 2025-09-23 南京师范大学 Cyp71p2酶、生物材料及应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1152029A (zh) * 1995-12-11 1997-06-18 浙江医科大学 人细胞色素p450系列转基因细胞株
CN1341631A (zh) * 2000-09-05 2002-03-27 上海博德基因开发有限公司 一种新的多肽——人细胞色素P450Cys亚铁血红素－铁蛋白9.13和编码这种多肽的多核苷酸
CA2954889A1 (en) * 2001-03-09 2002-09-19 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
CN1382707A (zh) * 2001-04-26 2002-12-04 上海博德基因开发有限公司 一种多肽——细胞色素p－450p－2－48.84和编码这种多肽的多核苷酸
KR20040091124A (ko) * 2002-03-12 2004-10-27 유.에스.스모크리스 토바코 컴퍼니 니코티아나 사이토크롬 ｐ450 유전자의 클로닝
AU2003301431A1 (en) * 2002-10-16 2004-05-04 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana

Also Published As

Publication number Publication date
JP2007508821A (ja) 2007-04-12
JP2010279377A (ja) 2010-12-16
CN102321641A (zh) 2012-01-18
PL1675957T3 (pl) 2012-06-29
WO2005038033A2 (en) 2005-04-28
EP1675957A1 (de) 2006-07-05
AU2010201695A1 (en) 2010-05-20
AU2010201696A1 (en) 2010-05-20
WO2005038033A8 (en) 2005-07-14
AU2004282576A1 (en) 2005-04-28
AU2010201696B2 (en) 2011-10-20
AU2010201695B2 (en) 2012-06-28
BRPI0415363A (pt) 2006-12-12
EP1675957B1 (de) 2011-12-07
ES2377868T3 (es) 2012-04-02
JP5721357B2 (ja) 2015-05-20
JP5422099B2 (ja) 2014-02-19
AU2004282576B2 (en) 2010-01-28

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