CN1210066C - 用来自分化细胞的供体核克隆猪的方法 - Google Patents

用来自分化细胞的供体核克隆猪的方法 Download PDF

Info

Publication number
CN1210066C
CN1210066C CNB988079569A CN98807956A CN1210066C CN 1210066 C CN1210066 C CN 1210066C CN B988079569 A CNB988079569 A CN B988079569A CN 98807956 A CN98807956 A CN 98807956A CN 1210066 C CN1210066 C CN 1210066C
Authority
CN
China
Prior art keywords
cells
disease
nuclear transfer
porcine
differentiated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNB988079569A
Other languages
English (en)
Chinese (zh)
Other versions
CN1265600A (zh
Inventor
S·L·斯迪司
J·M·罗伯尔
J·斯比里
P·格路克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Massachusetts Amherst
Original Assignee
University of Massachusetts Amherst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25392430&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN1210066(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of Massachusetts Amherst filed Critical University of Massachusetts Amherst
Publication of CN1265600A publication Critical patent/CN1265600A/zh
Application granted granted Critical
Publication of CN1210066C publication Critical patent/CN1210066C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Neurosurgery (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cardiology (AREA)
  • Diabetes (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Dermatology (AREA)
  • Heart & Thoracic Surgery (AREA)
CNB988079569A 1997-07-03 1998-07-01 用来自分化细胞的供体核克隆猪的方法 Expired - Lifetime CN1210066C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/888,057 1997-07-03
US08/888,057 US6235969B1 (en) 1997-01-10 1997-07-03 Cloning pigs using donor nuclei from non-quiescent differentiated cells

Publications (2)

Publication Number Publication Date
CN1265600A CN1265600A (zh) 2000-09-06
CN1210066C true CN1210066C (zh) 2005-07-13

Family

ID=25392430

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB988079569A Expired - Lifetime CN1210066C (zh) 1997-07-03 1998-07-01 用来自分化细胞的供体核克隆猪的方法

Country Status (10)

Country Link
US (4) US6235969B1 (https=)
EP (1) EP1017423A4 (https=)
JP (1) JP2001509362A (https=)
CN (1) CN1210066C (https=)
AU (1) AU742840B2 (https=)
BR (1) BR9811659A (https=)
CA (1) CA2295294A1 (https=)
IL (1) IL133785A0 (https=)
NZ (1) NZ502124A (https=)
WO (1) WO1999001164A1 (https=)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US7291764B1 (en) * 1997-01-10 2007-11-06 University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst Cloning pigs using non-quiescent differentiated donor cells or nuclei
US20090092588A1 (en) * 1997-01-10 2009-04-09 University Of Massachusetts As Represented By Its Amherst Campus Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US6331659B1 (en) 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
CA2324009A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd. Porcine nuclear transfer
JPH11289916A (ja) * 1998-04-09 1999-10-26 Kazuo Shiga 牛の筋肉組織の培養細胞を用いて受胎可能な体細胞核移植クローン胚の 作出及びクローン胚を凍結保存する方法
AUPP294898A0 (en) * 1998-04-15 1998-05-07 Monash University Method of nuclear transfer
WO2000022098A1 (en) 1998-10-12 2000-04-20 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
DK1159415T3 (da) 1999-03-04 2010-05-03 Revivicor Inc Genetisk modifikation af somatiske celler og anvendelse deraf
US6911429B2 (en) 1999-04-01 2005-06-28 Transition Therapeutics Inc. Compositions and methods for treating cellular response to injury and other proliferating cell disorders regulated by hyaladherin and hyaluronans
US6864235B1 (en) 1999-04-01 2005-03-08 Eva A. Turley Compositions and methods for treating cellular response to injury and other proliferating cell disorders regulated by hyaladherin and hyaluronans
JP2003507124A (ja) 1999-08-20 2003-02-25 メッツ−スタヴェンハーゲン，ペーター 脊柱セグメント
US20040180430A1 (en) * 1999-09-07 2004-09-16 West Michael D. Methods of restoring telomere length and extending cell lifespan using nuclear transfer
US20050255596A1 (en) * 1999-09-07 2005-11-17 West Michael D Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer
AU7372500A (en) * 1999-09-13 2001-04-17 University Of Massachusetts Cloning pigs using donor cells or nuclei from differentiated cells and production of pluripotent porcine.
WO2001030992A2 (en) * 1999-10-22 2001-05-03 University Of Pittsburgh Of The Commonwealth System Of Higher Education α1-3 GALACTOSYLTRANSFERASE GENE AND PROMOTER
KR100417566B1 (ko) * 1999-11-19 2004-02-05 한국생명공학연구원 체세포 핵치환 복제수정란의 대량생산방법
AU2766401A (en) * 2000-01-04 2001-07-16 University Of Connecticut, The Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
US20060174358A1 (en) * 2001-01-04 2006-08-03 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
ATE517990T1 (de) * 2000-03-15 2011-08-15 Univ Georgia Effektive kernumprogrammierung in säugetieren
US20040033600A1 (en) 2001-03-21 2004-02-19 Palli Subba Reddy Ecdysone receptor-based inducible gene expression system
AU2001247732A1 (en) * 2000-03-24 2001-10-08 Geron Corporation A strategy for maintaining pregnancy
GB2360522A (en) 2000-03-24 2001-09-26 Geron Corp A strategy for maintaining pregnancy
US20030172394A1 (en) * 2001-08-01 2003-09-11 Holzer George L Selection and cloning methods
EA013564B1 (ru) 2000-08-03 2010-06-30 Терапеутик Хьюман Поликлоналз Инк. Гуманизированный иммуноглобулин и содержащая его фармацевтическая композиция
US8105825B2 (en) * 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
DE60144248D1 (de) * 2000-12-22 2011-04-28 Kyowa Hakko Kirin Co Ltd Verfahren zur klonierung von nichtmenschliche säugern unter verwendung von reprogrammiertem donorchromatin oder donorzellen
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
MXPA03006751A (es) 2001-01-30 2005-04-08 Amy K Rebholtz Metodos para el manejo de recipientes para embriones.
ES2424812T3 (es) * 2001-02-20 2013-10-08 Intrexon Corporation Receptores X retinoides quiméricos y su uso en un sistema inducible de expresión génica basado en receptores de ecdisona novedoso
AU2002326353A1 (en) * 2001-07-09 2003-01-29 Alexion Pharmaceuticals Inc. Cloned non-human mammals from contact inhibited donor cells
CN1582332A (zh) * 2001-12-29 2005-02-16 Seoul大学校产学协力财团 转染gfp的克隆猪、敲除gt的克隆猪及其生产方法
AU2003235652A1 (en) * 2002-01-15 2003-07-30 Advanced Cell Technology, Inc. Cloning b and t lymphocytes
US20050170506A1 (en) * 2002-01-16 2005-08-04 Primegen Biotech Llc Therapeutic reprogramming, hybrid stem cells and maturation
US20050090004A1 (en) * 2003-01-16 2005-04-28 Sayre Chauncey B. Stem cell maturation for all tissue lines
US20030217378A1 (en) * 2002-04-05 2003-11-20 The University Of Georgia Research Foundation, Inc Cloning using rapidly matured oocytes
NZ536303A (en) * 2002-04-24 2008-04-30 Univ Georgia Res Found Production of cloned offspring from cooled carcasses
US7612250B2 (en) * 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
DK1534819T3 (da) * 2002-08-21 2010-04-19 Revivicor Inc Griselignende dyr, som mangler enhver ekspression af funktionel alfa-1,3-galactosyltransferase
US20050034175A1 (en) * 2002-09-09 2005-02-10 Latif Zuhair A. Immune cloning and uses thereof
US20040219563A1 (en) * 2002-10-16 2004-11-04 Michael West Method using gene trapped stem cells for making pathways of stem cell differentiation and making and isolating differentiated cells
US20040077077A1 (en) * 2002-10-18 2004-04-22 Xiangzhong Yang Novel methods for the production of cloned mammals, mammals cloned according to the methods, and methods of use of same
CN1683524A (zh) * 2003-04-08 2005-10-19 东南大学 用体细胞核移植技术制备移核胚胎干细胞的方法
EP1685148A2 (en) * 2003-11-05 2006-08-02 University Of Pittsburgh Of The Commonwealth System Of Higher Education PORCINE ISOGLOBOSIDE 3 SYNTHASE PROTEIN, cDNA, GENOMIC ORGANIZATION, AND REGULATORY REGION
CN104721883A (zh) 2004-03-17 2015-06-24 雷维维科公司 来源于缺乏任何功能性α1,3半乳糖基转移酶表达的动物的组织产品
US20050277147A1 (en) * 2004-06-11 2005-12-15 Ameet Patki Identifying chromosomal abnormalities in cells obtained from follicular fluid
US20060080746A1 (en) * 2004-09-28 2006-04-13 Viagen, Inc. Methods of embryo transfer
KR100733012B1 (ko) * 2005-07-26 2007-06-28 재단법인서울대학교산학협력재단 복제된 개과 동물 및 이의 생산 방법
CA2909775A1 (en) 2005-08-09 2007-03-29 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
WO2008085891A1 (en) * 2007-01-05 2008-07-17 Worcester Polytechnic Institute Oocyte spindle-associated factors improve somatic cell cloning
KR100829426B1 (ko) 2007-01-17 2008-05-15 재단법인서울대학교산학협력재단 복제개의 생산 방법
KR100868245B1 (ko) 2007-05-29 2008-11-11 강원대학교산학협력단 데메콜친 처리에 의한 효율적인 복제 동물의 생산방법
CA3104704A1 (en) * 2009-08-14 2011-02-17 Revivicor, Inc. Multi-transgenic pigs for diabetes treatment
CN103492576A (zh) 2011-02-14 2014-01-01 雷维维科公司 用于血管化异种移植物及其衍生物的异种移植的遗传修饰的猪
CN103173456B (zh) * 2012-12-14 2015-09-23 华南农业大学 猪Xist基因序列、猪anti-Xist siRNA序列及通过抑制猪Xist基因表达来提高猪克隆效率的方法
DK2993234T3 (en) 2013-04-30 2019-01-07 Univ Konkuk Ind Coop Corp Vector Targeted CMP Acetylneuramic Acid Hydroxylase, Transgenic Animal for Xenotransplantation Introduced with the Vector and Method of Preparation thereof
CN103343142B (zh) * 2013-07-15 2017-07-25 云南农业大学 一种利用连续克隆技术生产孤雌生殖克隆猪的方法
JP6929527B2 (ja) * 2015-03-04 2021-09-01 株式会社ポル・メド・テック 安定した表現型を示す疾患モデルブタおよびその作製方法
JP2023551404A (ja) 2020-11-20 2023-12-08 レビビコア， インコーポレイテッド 異種間移植のための成長ホルモン受容体ノックアウトを有する多重トランスジェニックブタ
CN112759633B (zh) * 2021-01-07 2022-08-12 江西农业大学 一种可用于建立猪癌症模型的蛋白及其核酸
KR20240099180A (ko) 2021-09-20 2024-06-28 레비비코르 인코포레이션 이종이식을 위한 10개의 유전자 변형을 포함하는 다중전이유전자 돼지

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994384A (en) * 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) * 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
GB9003791D0 (en) 1990-02-20 1990-04-18 Ludwig Inst Cancer Res Transgenic animals,cell lines therefrom,and their use
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
JPH09500526A (ja) 1993-06-14 1997-01-21 ベーアーエスエフ アクツィエンゲゼルシャフト テトラサイクリン反応性プロモーターによる真核細胞の遺伝子発現の厳密な制御
AU1439295A (en) * 1993-12-17 1995-07-03 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
EP0739412B1 (en) * 1993-12-23 2002-02-27 Infigen, Inc. ungulate EMBRYONIC STEM CELLS AS NUCLEAR DONORS AND NUCLEAR TRANSFER TECHNIQUES TO PRODUCE CHIMERIC AND TRANSGENIC ANIMALS
DE69535752D1 (de) * 1994-04-13 2008-06-26 Biotransplant Inc Alpha(1,3)galactosyltransferase-negatives schwein
AUPM621394A0 (en) * 1994-06-10 1994-07-07 Mitchell, John Cathode connection for aluminium smelter pot
GB9417831D0 (en) * 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation
US7307198B2 (en) * 1995-08-31 2007-12-11 Roslin Institute Ungulates produced by nuclear transfer of G1 cells
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US20020012655A1 (en) * 1997-01-10 2002-01-31 Steven L. Stice Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US6215041B1 (en) * 1997-01-10 2001-04-10 University Of Mmassachusetts Cloning using donor nuclei from a non-quiesecent somatic cells
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US20010039667A1 (en) * 1997-01-10 2001-11-08 University Of Massachusetts Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease
IL133786A0 (en) * 1997-07-03 2001-04-30 Univ Massachusetts Cloning using donor nuclei from non-serum starved, differentiated cells
WO1999021415A1 (en) * 1997-10-28 1999-05-06 Stem Cell Sciences Pty. Ltd. Nuclear transfer for production of transgenic animal embryo
US6675205B2 (en) * 1999-10-14 2004-01-06 Arcessa, Inc. Peer-to-peer automated anonymous asynchronous file sharing
US6789132B2 (en) * 2000-02-09 2004-09-07 Seagate Technology Llc Modular disc drive architecture
US20040194158A1 (en) * 2003-03-28 2004-09-30 Baylor College Of Medicine Model for neurodegenerative disorders

Also Published As

Publication number Publication date
JP2001509362A (ja) 2001-07-24
BR9811659A (pt) 2000-09-05
AU8374598A (en) 1999-01-25
US20020035737A1 (en) 2002-03-21
IL133785A0 (en) 2001-04-30
NZ502124A (en) 2003-01-31
WO1999001164A1 (en) 1999-01-14
EP1017423A1 (en) 2000-07-12
CA2295294A1 (en) 1999-01-14
US20070067861A1 (en) 2007-03-22
US20040194159A1 (en) 2004-09-30
AU742840B2 (en) 2002-01-10
US6235969B1 (en) 2001-05-22
CN1265600A (zh) 2000-09-06
EP1017423A4 (en) 2002-01-30

Similar Documents

Publication Publication Date Title
CN1210066C (zh) 用来自分化细胞的供体核克隆猪的方法
AU742363B2 (en) Nuclear transfer with differentiated fetal and adult donor cells
CN1265599A (zh) 使用来自无血清饥饿的分化细胞的供体细胞核进行克隆
CN1200107C (zh) 以成年体细胞胞核重建的去核卵母细胞足月发育成动物
JP2001500725A (ja) 種間核移植により製造される胚性または幹細胞様細胞株
AU2211499A (en) Cloning using donor nuclei from differentiated fetal and adult cells
CN1219198A (zh) 来自有蹄动物胚胎的培养内细胞团细胞系
CN1391605A (zh) 多能细胞和细胞系的单雌生殖或单雄生殖产生,及其产生分化细胞和组织的用途
CN1299408A (zh) 通过异种间的核移植产生的胚细胞或干细胞样细胞系
CN1425064A (zh) 通过交叉物种核移植制备的胚胎或干样细胞
CN1298841C (zh) 重建胚胎中核转移后融合和活化的方法和系统
CN1399513A (zh) 用于核移植的供体细胞的制备和选择
JP2003509031A (ja) ドナー細胞又は分化細胞からの核を使用する豚のクローニング並びに多能性豚の産生
CN1424870A (zh) 用挑选的供体细胞实施核移植
CN1284851C (zh) 体细胞起源的胚胎干细胞及其分化细胞
CN1650003A (zh) 选择用于哺乳动物种中核转移的细胞系的方法
CN1673361A (zh) 含有异源核和线粒体的多能细胞
AU2011202964A1 (en) Nuclear transfer with differentiated fetal and adult donor cells
AU2006236032A1 (en) Nuclear transfer with differentiated fetal and adult donor cells
AU2006202185A1 (en) Cloning using donor nuclei from non-serum starved, differentiated cells

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20050713