WO1999001164A1 - Cloning pigs using donor nuclei from differentiated cells - Google Patents

Cloning pigs using donor nuclei from differentiated cells Download PDF

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Publication number
WO1999001164A1
WO1999001164A1 PCT/US1998/012806 US9812806W WO9901164A1 WO 1999001164 A1 WO1999001164 A1 WO 1999001164A1 US 9812806 W US9812806 W US 9812806W WO 9901164 A1 WO9901164 A1 WO 9901164A1
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WO
WIPO (PCT)
Prior art keywords
cell
disease
offspring
cells
pig
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Ceased
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PCT/US1998/012806
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English (en)
French (fr)
Inventor
Steven L. Stice
James M. Robl
Jose Cibelli
Paul Golueke
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University of Massachusetts Amherst
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University of Massachusetts Amherst
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25392430&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1999001164(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of Massachusetts Amherst filed Critical University of Massachusetts Amherst
Priority to IL13378598A priority Critical patent/IL133785A0/xx
Priority to BR9811659-2A priority patent/BR9811659A/pt
Priority to NZ502124A priority patent/NZ502124A/xx
Priority to EP98934152A priority patent/EP1017423A4/en
Priority to JP2000500927A priority patent/JP2001509362A/ja
Priority to AU83745/98A priority patent/AU742840B2/en
Priority to CA002295294A priority patent/CA2295294A1/en
Publication of WO1999001164A1 publication Critical patent/WO1999001164A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
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    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Definitions

  • ES cells from livestock animals are highly desirable because they may provide a potential source of totipotent donor nuclei, genetically manipulated or otherwise, for nuclear transfer procedures.
  • cell nuclei derived from differentiated pig cells are transplanted into enucleated pig oocytes .
  • the nuclei are reprogrammed to direct the development of cloned embryos, which can then be transferred into recipient females to produce fetuses and offspring, or used to produce CICM cells.
  • the cloned embryos can also be combined with fertilized embryos to produce chimeric embryos, fetuses and/or offspring.
  • Enucleation may be effected by known methods, such as described in U.S. Patent No. 4,994,384 which is incorporated by reference herein.
  • metaphase II oocytes are either placed in HECM, optionally containing 7.5 micrograms per milliliter cytochalasin B (CB) and 0.15 M sucrose, for immediate enucleation, or may be placed in a suitable medium, for example an embryo culture medium such as NCSU 23 (see Table in the Examples) at 39°C and 5% C0 2 , and then enucleated later, preferably not more than 24 hours later, and more preferably immediately.
  • CB cytochalasin B
  • NCSU 23 see Table in the Examples

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  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Biomedical Technology (AREA)
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  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
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  • Neurosurgery (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cardiology (AREA)
  • Diabetes (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Dermatology (AREA)
  • Heart & Thoracic Surgery (AREA)
PCT/US1998/012806 1997-07-03 1998-07-01 Cloning pigs using donor nuclei from differentiated cells Ceased WO1999001164A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
IL13378598A IL133785A0 (en) 1997-07-03 1998-07-01 Cloning pigs using donor nuclei from differentiated cells
BR9811659-2A BR9811659A (pt) 1997-07-03 1998-07-01 Clonagem de porcos usando-se núcleos doadores de células diferenciadas
NZ502124A NZ502124A (en) 1997-07-03 1998-07-01 Cloning pigs using donor nuclei from differentiated cells
EP98934152A EP1017423A4 (en) 1997-07-03 1998-07-01 CLONING OF PIGS USING DONOR CORES FROM DIFFERENTIATED CELLS
JP2000500927A JP2001509362A (ja) 1997-07-03 1998-07-01 分化細胞からのドナー核を使用するブタのクローニング
AU83745/98A AU742840B2 (en) 1997-07-03 1998-07-01 Cloning pigs using donor nuclei from differentiated cells
CA002295294A CA2295294A1 (en) 1997-07-03 1998-07-01 Cloning pigs using donor nuclei from differentiated cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/888,057 1997-07-03
US08/888,057 US6235969B1 (en) 1997-01-10 1997-07-03 Cloning pigs using donor nuclei from non-quiescent differentiated cells

Publications (1)

Publication Number Publication Date
WO1999001164A1 true WO1999001164A1 (en) 1999-01-14

Family

ID=25392430

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/012806 Ceased WO1999001164A1 (en) 1997-07-03 1998-07-01 Cloning pigs using donor nuclei from differentiated cells

Country Status (10)

Country Link
US (4) US6235969B1 (https=)
EP (1) EP1017423A4 (https=)
JP (1) JP2001509362A (https=)
CN (1) CN1210066C (https=)
AU (1) AU742840B2 (https=)
BR (1) BR9811659A (https=)
CA (1) CA2295294A1 (https=)
IL (1) IL133785A0 (https=)
NZ (1) NZ502124A (https=)
WO (1) WO1999001164A1 (https=)

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer
WO1999053025A1 (en) * 1998-04-15 1999-10-21 Monash University Method of nuclear transfer
WO2000022098A1 (en) * 1998-10-12 2000-04-20 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
WO2001013826A1 (de) * 1999-08-20 2001-03-01 Metz Stavenhagen Peter Wirbelsäulensegment
US6331659B1 (en) 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
WO2001072120A3 (en) * 2000-03-24 2002-02-14 Geron Corp A strategy for maintaining pregnancy
JP2003509031A (ja) * 1999-09-13 2003-03-11 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス ドナー細胞又は分化細胞からの核を使用する豚のクローニング並びに多能性豚の産生
WO2002074078A3 (en) * 2001-01-30 2003-09-25 Rebholtz Amy K Lf Method for embryo after impregnation of the recipient female
US6673987B1 (en) 2000-03-24 2004-01-06 Geron Corporation Strategy for maintaining pregnancy
AU769618B2 (en) * 1999-11-19 2004-01-29 Korea Research Institute Of Bioscience And Biotechnology An improved method for production of porcine clone embryos via somatic cell nuclear transfer
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
US6864235B1 (en) 1999-04-01 2005-03-08 Eva A. Turley Compositions and methods for treating cellular response to injury and other proliferating cell disorders regulated by hyaladherin and hyaluronans
US6911429B2 (en) 1999-04-01 2005-06-28 Transition Therapeutics Inc. Compositions and methods for treating cellular response to injury and other proliferating cell disorders regulated by hyaladherin and hyaluronans
US7129084B2 (en) 2000-08-03 2006-10-31 Therapeutic Human Polyclonals, Inc. Production of humanized antibodies in transgenic animals
WO2007013763A1 (en) * 2005-07-26 2007-02-01 Seoul National University Industry Foundation Cloned canines and method for producing thereof
US7291764B1 (en) 1997-01-10 2007-11-06 University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst Cloning pigs using non-quiescent differentiated donor cells or nuclei
US7601884B2 (en) 2007-05-29 2009-10-13 Knu - Industry Cooperation Foundation Method of producing cloned animals by demecolcine treatment
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
EP2199392A2 (en) 1999-03-04 2010-06-23 Revivicor, Inc. Genetic modification of somatic cells and uses thereof
US8124832B2 (en) 2007-01-17 2012-02-28 Seoul National University Industry Foundation Method for producing cloned dog

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US20090092588A1 (en) * 1997-01-10 2009-04-09 University Of Massachusetts As Represented By Its Amherst Campus Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
JPH11289916A (ja) * 1998-04-09 1999-10-26 Kazuo Shiga 牛の筋肉組織の培養細胞を用いて受胎可能な体細胞核移植クローン胚の 作出及びクローン胚を凍結保存する方法
US20040180430A1 (en) * 1999-09-07 2004-09-16 West Michael D. Methods of restoring telomere length and extending cell lifespan using nuclear transfer
US20050255596A1 (en) * 1999-09-07 2005-11-17 West Michael D Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer
WO2001030992A2 (en) * 1999-10-22 2001-05-03 University Of Pittsburgh Of The Commonwealth System Of Higher Education α1-3 GALACTOSYLTRANSFERASE GENE AND PROMOTER
AU2766401A (en) * 2000-01-04 2001-07-16 University Of Connecticut, The Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
US20060174358A1 (en) * 2001-01-04 2006-08-03 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
ATE517990T1 (de) * 2000-03-15 2011-08-15 Univ Georgia Effektive kernumprogrammierung in säugetieren
US20040033600A1 (en) 2001-03-21 2004-02-19 Palli Subba Reddy Ecdysone receptor-based inducible gene expression system
US20030172394A1 (en) * 2001-08-01 2003-09-11 Holzer George L Selection and cloning methods
US8105825B2 (en) * 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
DE60144248D1 (de) * 2000-12-22 2011-04-28 Kyowa Hakko Kirin Co Ltd Verfahren zur klonierung von nichtmenschliche säugern unter verwendung von reprogrammiertem donorchromatin oder donorzellen
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
ES2424812T3 (es) * 2001-02-20 2013-10-08 Intrexon Corporation Receptores X retinoides quiméricos y su uso en un sistema inducible de expresión génica basado en receptores de ecdisona novedoso
AU2002326353A1 (en) * 2001-07-09 2003-01-29 Alexion Pharmaceuticals Inc. Cloned non-human mammals from contact inhibited donor cells
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