CN1167811C - 酶原性核酸的检测方法,以及相关分子和试剂盒 - Google Patents

酶原性核酸的检测方法,以及相关分子和试剂盒 Download PDF

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CN1167811C
CN1167811C CNB998111147A CN99811114A CN1167811C CN 1167811 C CN1167811 C CN 1167811C CN B998111147 A CNB998111147 A CN B998111147A CN 99811114 A CN99811114 A CN 99811114A CN 1167811 C CN1167811 C CN 1167811C
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nucleic acid
target
primer
sample
dna
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CN1319142A (zh
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A���е�
A·托德
C·J·富尔里
M·凯恩斯
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Johnson and Johnson Research Pty Ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB998111147A 1998-03-05 1999-02-22 酶原性核酸的检测方法,以及相关分子和试剂盒 Expired - Fee Related CN1167811C (zh)

Applications Claiming Priority (2)

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US7689998P 1998-03-05 1998-03-05
US60/076,899 1998-03-05

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CN1319142A CN1319142A (zh) 2001-10-24
CN1167811C true CN1167811C (zh) 2004-09-22

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US (3) US6140055A (https=)
EP (1) EP1062367B1 (https=)
JP (1) JP4318856B2 (https=)
KR (1) KR100748863B1 (https=)
CN (1) CN1167811C (https=)
AT (1) ATE414169T1 (https=)
AU (1) AU768627B2 (https=)
BR (1) BR9908567A (https=)
CA (1) CA2321948A1 (https=)
DE (1) DE69939896D1 (https=)
IL (2) IL164182A0 (https=)
WO (1) WO1999045146A1 (https=)
ZA (1) ZA991772B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103013953A (zh) * 2012-10-19 2013-04-03 中国科学院成都生物研究所 一种剪切dna的dna酶

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
IL164182A0 (en) 1998-03-05 2005-12-18 Johnson & Johnson Res Pty Ltd Zymogenic nucleic acid detection methods, and related molecules and kits
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
AU2001268481A1 (en) * 2000-06-15 2001-12-24 Board Of Regents, The University Of Texas System Regulatable, catalytically active nucleic acids
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
WO2003014375A2 (en) * 2001-08-09 2003-02-20 Archemix Corporation Nucleic acid sensor molecules and methods of using same
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
US20050250134A1 (en) * 2004-03-02 2005-11-10 Dawei Sheng Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2006086499A2 (en) * 2005-02-08 2006-08-17 Applera Corporation Nucleozymes and methods of use
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
JP2008539730A (ja) * 2005-05-02 2008-11-20 ストラタジーン カリフォルニア オリゴヌクレオチドのプローブ／プライマー組成物、及びポリヌクレオチドの検出方法
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP2385141B1 (en) 2005-10-07 2013-08-07 SpeeDx Pty Ltd Multicomponent nucleic acid enzymes and methods for their use
WO2007056312A2 (en) * 2005-11-07 2007-05-18 Clontech Laboratories, Inc. Universal reporter tag for nucleozyme assays
CA2631881C (en) * 2005-12-07 2015-03-24 Tibotec Pharmaceuticals Ltd. Methods, plasmid vectors and primers for assessing hiv viral fitness
WO2008001376A2 (en) * 2006-06-28 2008-01-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem Detection of analytes in a medium
DE102006035600B3 (de) * 2006-07-27 2007-11-08 Epigenomics Ag Verfahren zum Nachweis eines Methylierungsmusters
EP1882746A3 (en) * 2006-07-27 2008-03-19 Epigenomics AG Method for detecting a methylation pattern
JP5688507B2 (ja) * 2006-10-06 2015-03-25 スピーデクス ピーティーワイ リミテッド 分子スイッチおよびそれらの使用のための方法
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
MX2009010710A (es) 2007-04-05 2009-12-08 Johnson & Johnson Res Pty Ltd Complejos y enzimas de acido nucleico y metodos para su uso.
EP2071035A1 (en) 2007-12-13 2009-06-17 Epigenomics AG Facilitator and method for amplification
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
CN101907573B (zh) * 2010-08-17 2012-08-22 复旦大学 一种高灵敏度的集成cmos光学生物芯片
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
JP6088487B2 (ja) 2011-04-15 2017-03-01 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 走査リアルタイムマイクロ流体熱サイクラーと同期熱サイクリング及び走査光学検出の方法
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
US20160202154A1 (en) * 2013-09-05 2016-07-14 Nec Solution Innovators, Ltd. Method for producing sample and method for analyzing target

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4000159A (en) * 1974-06-28 1976-12-28 Phillips Petroleum Company Preparation of n,n-disubstituted thioamides
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
NZ241310A (en) * 1991-01-17 1995-03-28 Gen Hospital Corp Trans-splicing ribozymes
GB9115909D0 (en) * 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
EP1195442A3 (en) * 1992-12-04 2002-07-31 Innovir Laboratories, Inc. Ribozyme amplified diagnostics
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
KR100218726B1 (ko) * 1992-12-30 1999-09-01 김영환 고집적 반도체 소자의 접속장치 및 그 제조방법
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
IL111857A (en) * 1994-12-02 1999-09-22 Intelligene Ltd Detection of nucleic acid sequences
MX9706501A (es) * 1995-02-27 1998-02-28 Intelligene Ltd Deteccion de biomoleculas.
AUPN245295A0 (en) * 1995-04-13 1995-05-11 Johnson & Johnson Research Pty. Limited Assay for genetic abnormalities
EP0833945B1 (en) * 1995-06-07 2002-01-30 Merck & Co., Inc. Dna polymerase extension assay
JP4203126B2 (ja) * 1997-04-29 2008-12-24 ザ スクリップス リサーチ インスティテュート 酵素ｄｎａ分子
DE19805593A1 (de) * 1998-02-12 1999-08-19 Voith Sulzer Papiertech Patent Eindicker
IL164182A0 (en) 1998-03-05 2005-12-18 Johnson & Johnson Res Pty Ltd Zymogenic nucleic acid detection methods, and related molecules and kits

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103013953A (zh) * 2012-10-19 2013-04-03 中国科学院成都生物研究所 一种剪切dna的dna酶
CN103013953B (zh) * 2012-10-19 2014-09-17 中国科学院成都生物研究所 一种剪切dna的dna酶

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AU768627B2 (en) 2003-12-18
ZA991772B (en) 2000-10-12
US6140055A (en) 2000-10-31
EP1062367A1 (en) 2000-12-27
JP2002505117A (ja) 2002-02-19
US6201113B1 (en) 2001-03-13
US6365724B2 (en) 2002-04-02
WO1999045146A1 (en) 1999-09-10
AU3340999A (en) 1999-09-20
ATE414169T1 (de) 2008-11-15
EP1062367A4 (en) 2003-07-30
JP4318856B2 (ja) 2009-08-26
KR100748863B1 (ko) 2007-08-13
IL138126A0 (en) 2001-10-31
IL164182A0 (en) 2005-12-18
CN1319142A (zh) 2001-10-24
KR20010041591A (ko) 2001-05-25
EP1062367B1 (en) 2008-11-12
US20010001063A1 (en) 2001-05-10
BR9908567A (pt) 2000-12-12
DE69939896D1 (de) 2008-12-24
CA2321948A1 (en) 1999-09-10

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