CN115443894A - Large-scale three-dimensional cultivation method and cultivation system for dendrobium officinale tissue culture seedlings in greenhouse - Google Patents
Large-scale three-dimensional cultivation method and cultivation system for dendrobium officinale tissue culture seedlings in greenhouse Download PDFInfo
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- CN115443894A CN115443894A CN202211123113.1A CN202211123113A CN115443894A CN 115443894 A CN115443894 A CN 115443894A CN 202211123113 A CN202211123113 A CN 202211123113A CN 115443894 A CN115443894 A CN 115443894A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
Abstract
The invention discloses a large-scale three-dimensional cultivation method and a cultivation system for tissue culture seedlings of dendrobium officinale in a greenhouse, wherein the large-scale three-dimensional cultivation method for the tissue culture seedlings of the dendrobium officinale in the greenhouse comprises the following steps of installing a plurality of vertical surface support frames in the greenhouse, enabling the vertical surface support frames to be provided with a first vertical surface and a second vertical surface, fixing barks on the first vertical surface and the second vertical surface, enabling the surfaces of the barks to deviate from the first vertical surface and the second vertical surface, flatly paving the tissue culture seedlings of the dendrobium officinale meeting preset conditions on the barks, packaging pine bark powder into non-woven fabric packaging paper, placing the non-woven fabric packaging paper on the roots of the tissue culture seedlings of the dendrobium officinale, fixing the tissue culture seedlings of the dendrobium officinale on the vertical surfaces of the barks by using palm fibers, and spraying and fertilizing the tissue culture seedlings of the dendrobium officinale by using a vertical surface spraying device. The method can improve the stress resistance and the water and fertilizer utilization efficiency of the dendrobium officinale tissue culture seedling, and the hardening and planting links of the tissue culture seedling are directly arranged on the vertical surface, so that the aim of large-scale vertical surface cultivation of the dendrobium officinale tissue culture seedling is fulfilled.
Description
Technical Field
The invention relates to the technical field of plant three-dimensional cultivation, in particular to a large-scale three-dimensional cultivation method and system for dendrobium officinale tissue culture seedlings in a greenhouse.
Background
The dendrobium officinale is a traditional rare epiphytic herbal traditional Chinese medicine, and the stem of the dendrobium officinale is a medicinal part containing main effective components of dendrobium polysaccharides and alkaloids, and has the effects of improving immunity, resisting tumors, inhibiting aging and the like. The wild dendrobium officinale needs strict environmental conditions for growth, and artificial excessive mining and ecological damage cause that the dendrobium officinale is more difficult to propagate, survive and grow. In order to meet the market demand of the dendrobium officinale, most of the dendrobium officinale in the market at present are produced by producing tissue culture seedlings and then domesticating and cultivating the tissue culture seedlings on a greenhouse seedbed, the culture medium needs to be continuously replaced during the period of hardening and planting of the tissue culture seedlings of the dendrobium officinale, the operation is complex, the cultivation cost is increased, in addition, the stress resistance of the tissue culture seedlings of the dendrobium officinale cultivated by the existing seedbed medium is poor, the water and fertilizer utilization efficiency of the tissue culture seedling seedbed medium cultivation scheme is low, the diseases are difficult to control, and the survival rate of the tissue culture seedlings of the dendrobium officinale in hardening and planting is low. At present, the existing three-dimensional cultivation technology can effectively solve part of the problems, but how to operate the hardening off and planting processes of the dendrobium officinale tissue culture seedlings on a large scale on greenhouse three-dimensional cultivation is not explored.
Disclosure of Invention
Therefore, a large-scale three-dimensional cultivation method and a large-scale three-dimensional cultivation system for the dendrobium officinale tissue culture seedlings in the greenhouse are needed to be provided, so that the problem of how to effectively acclimatize and fix the dendrobium officinale tissue culture seedlings in the greenhouse in a large scale through a three-dimensional cultivation technology is solved.
The invention provides a large-scale three-dimensional cultivation method of dendrobium officinale tissue culture seedlings in a greenhouse, which comprises the following steps:
the method comprises the following steps that a plurality of vertical face support frames are installed in a greenhouse in a large-scale mode, and each vertical face support frame is provided with a first vertical face and a second vertical face;
fixing bark on the first vertical surface and the second vertical surface in a large scale, wherein the surface of the bark is away from the first vertical surface and the second vertical surface;
flatly paving the dendrobium officinale tissue culture seedlings meeting the preset conditions on the barks;
packaging the pine bark powder into non-woven fabric packaging paper;
placing the non-woven fabric wrapping paper on the root of the dendrobium officinale tissue culture seedling;
and sprinkling water and applying fertilizer to the dendrobium officinale tissue culture seedlings by the vertical spraying device.
Further, the step of paving the dendrobium officinale tissue culture seedlings meeting the preset conditions on the bark comprises the following steps:
selecting dendrobium officinale tissue culture seedlings containing 5-7 true leaves and part of the dendrobium officinale tissue culture seedlings with root lengths of 3-4 cm;
cleaning the dendrobium officinale tissue culture seedlings;
spreading the cleaned dendrobium officinale tissue culture seedling on a seedbed until the root base of the dendrobium officinale tissue culture seedling is white;
and spreading the dendrobium officinale tissue culture seedlings with white root parts on the barks from the seedbed according to preset arrangement.
Further, the preset arrangement is that 3-4 dendrobium officinale tissue culture seedlings are in one cluster, 12-14 clusters of dendrobium officinale tissue culture seedlings are in 1 row, and 6-8 rows of dendrobium officinale tissue culture seedlings are arranged on the first vertical surface or the second vertical surface.
Further, fixing the dendrobium officinale tissue culture seedling meeting the preset conditions and the non-woven fabric filled with the pine bark powder on the bark through the palm fibers or the palm ropes.
Further, the step of setting up the spraying device that is used for watering and fertilizeing dendrobium officinale tissue culture seedling includes:
a first rail and a second rail which are opposite to each other are laid between the two vertical face supporting frames;
a vertical face spraying rail car is arranged on the first rail and the second rail in a sliding mode;
a liquid storage tank for storing water and liquid fertilizer is arranged on the spraying rail car;
and driving the vertical surface spraying rail car to move on the first rail and the second rail, and spraying water and liquid fertilizer in the liquid storage tank to the dendrobium officinale tissue culture seedlings on the vertical surface support frame.
Further, the step of fixing the bark on the first vertical surface and the second vertical surface further comprises:
the barks are steamed and boiled in a steam sterilizer for 30 to 40 minutes.
Further, the length of the bark is 1-1.4 m, the width is 0.4-0.6 m, and the thickness is 0.4-0.6 cm.
Further, the bark is whole bark of big fir directly processed and stripped from the wood of adult fir.
The invention provides a cultivation system applied to the three-dimensional cultivation method, which comprises the following steps:
the vertical surface support frames are provided with a first vertical surface and a second vertical surface, and the first vertical surface and the second vertical surface are both provided with a seedling planting area consisting of barks;
the first rail and the second rail are arranged between two opposite vertical surface supporting frames; and
the elevation spraying device is arranged on the first track and the second track in a sliding mode and used for spraying water and liquid fertilizer to the dendrobium officinale tissue culture seedlings on the elevation supporting frame.
Furthermore, the distance between two opposite vertical surface support frames is 1.8-2 m.
According to the large-scale three-dimensional cultivation method for the dendrobium officinale tissue culture seedlings in the greenhouse, pine bark powder is packaged into non-woven fabric packaging paper, the non-woven fabric packaging paper is placed on the roots of the dendrobium officinale tissue culture seedlings, the dendrobium officinale tissue culture seedlings are fixed on the bark vertical surfaces through palm fibers, after the water spraying and the fertilizer application are carried out on the dendrobium officinale tissue culture seedlings through a vertical surface spraying device, the pine bark powder in the non-woven fabric can slowly release pine natural metabolites promoting the growth and the stress resistance of the dendrobium officinale tissue culture seedlings, after the pine bark powder is absorbed by the roots of the dendrobium officinale tissue culture seedlings, the stress resistance of the dendrobium officinale tissue culture seedlings is improved, and the higher water and fertilizer utilization efficiency of vertical surface cultivation is combined, so that the aims of carrying out vertical surface hardening and field planting on the dendrobium officinale tissue culture seedlings in the greenhouse in a large-scale mode are achieved.
Drawings
In order to more clearly illustrate the embodiments or technical solutions of the present invention, the drawings used in the embodiments or technical solutions of the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a flow chart of a large-scale three-dimensional cultivation method of a greenhouse dendrobium officinale tissue culture seedling in the embodiment of the invention.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all the directional indicators (such as upper, lower, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, the descriptions relating to "first", "second", etc. in the present invention are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In addition, "and/or" in the whole text includes three schemes, taking a and/or B as an example, including a technical scheme, and a technical scheme that a and B meet simultaneously; in addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
As shown in fig. 1, in some embodiments, a large-scale three-dimensional cultivation method for tissue culture seedlings of dendrobium officinale in a greenhouse comprises the following steps:
s100, a plurality of vertical surface support frames are installed in the greenhouse in a large-scale mode, and each vertical surface support frame is provided with a first vertical surface and a second vertical surface.
S200, fixing the barks on the first vertical surface and the second vertical surface in a large scale, wherein the surfaces of the barks are deviated from the first vertical surface and the second vertical surface.
S300, paving the dendrobium officinale tissue culture seedlings meeting the preset conditions on the barks.
S400, packaging the pine bark powder into non-woven fabric packaging paper.
S500, placing the non-woven fabric packaging paper on the roots of the dendrobium officinale tissue culture seedlings, and fixing the non-woven fabric packaging paper on the bark vertical surface by using palm fibers.
S600, spraying water and fertilizing the dendrobium officinale tissue culture seedlings through a vertical spraying device.
After watering and fertilization are carried out to dendrobium officinale tissue culture seedlings through facade spraying device, the pine bark powder in the non-woven fabrics can slowly release pine natural metabolites promoting the growth of the dendrobium officinale tissue culture seedlings and resisting reverse, and after the absorption of the roots of the dendrobium officinale tissue culture seedlings, the stress resistance of the dendrobium officinale tissue culture seedlings is improved, and the higher water and fertilizer utilization efficiency of facade cultivation is combined, so that the aims of facade hardening and field planting of the dendrobium officinale tissue culture seedlings in a greenhouse in a large scale mode are fulfilled.
In addition, the dendrobium officinale is cultivated on trees or stakes or wood heads in the existing three-dimensional cultivation technology, but the materials related to the schemes are heavy, the size is large, the carrying and the replacement of dendrobium seedlings are inconvenient, the space utilization rate of the dendrobium officinale cultivation is low, the manual water and fertilizer management is very inconvenient, and the cultivation cost is increased. The tissue culture seedlings of the dendrobium officinale are flatly paved on the barks, the barks are relatively portable and can be standardized relative to the whole trees or wooden piles or wood, the transport or the replacement of the tissue culture seedlings of the dendrobium officinale are convenient, the first vertical face and the second vertical face are arranged on the vertical face support frame in a large-scale mode, the space utilization rate and the water and fertilizer of the dendrobium officinale cultivation are improved, and the cultivation cost is reduced.
Optionally, the length of the bark is 1-1.4 m, the width is 0.4-0.6 m, and the thickness is 0.4-0.6 cm. The bark is the whole bark of the big fir directly processed and stripped from the wood of the adult fir.
Specifically, the step of flatly paving the dendrobium officinale tissue culture seedlings meeting the preset conditions on the barks comprises the following steps of:
s310, selecting the dendrobium officinale tissue culture seedling which contains 5-7 true leaves and part of which the root length reaches 3-4 cm.
S320, cleaning the dendrobium officinale tissue culture seedlings.
S330, laying the cleaned dendrobium officinale tissue culture seedlings on a seedbed until the root bases of the dendrobium officinale tissue culture seedlings are white.
S340, spreading the dendrobium officinale tissue culture seedlings with white root parts on the bark from the seedbed according to preset arrangement.
More specifically, the preset arrangement is that 3-4 dendrobium officinale tissue culture seedlings are in one cluster, 12-14 clusters of dendrobium officinale tissue culture seedlings are in 1 row, and 6-8 rows of dendrobium officinale tissue culture seedlings are arranged on the first vertical surface or the second vertical surface.
Further, S350, fixing the dendrobium officinale tissue culture seedling meeting the preset conditions and the non-woven fabric containing the pine tree powder on the bark through the palm fibers or the palm ropes. In the prior art, dendrobium officinale is fixed through hemp ropes or hemp cloths, but the hemp ropes or hemp cloths are easy to corrode after being used for a long time, and the fixation of the dendrobium officinale tissue culture seedlings through palm fibers or palm ropes has obvious corrosion resistance and durability.
Further, S500, the step of arranging a spraying device for spraying water and fertilizing the dendrobium officinale tissue culture seedlings comprises the following steps:
s510, paving a first rail and a second rail which are opposite to each other between the two vertical face supporting frames;
s520, a vertical spraying rail car is slidably arranged on the first rail and the second rail;
s530, installing a liquid storage tank for storing water and liquid fertilizer on the vertical spraying rail car;
s540, driving the vertical spraying rail car to move on the first rail and the second rail, and spraying water and liquid fertilizer in the liquid storage tank on the dendrobium officinale tissue culture seedlings on the vertical supporting frame.
Optionally, S200, before the step of fixing the bark on the first vertical surface and the second vertical surface, further comprises:
s600, steaming the barks in a steam sterilizer for 30-40 minutes to sterilize the barks of the Chinese fir and wet the barks of the Chinese fir.
Optionally, 2300-2600 clumps of dendrobium officinale tissue culture seedlings can be cultivated by one vertical support frame with the length of 20 meters, and the cultivation survival rate of the dendrobium officinale tissue culture seedlings reaches 92%.
Optionally, the shed roof is preferably covered with a plastic film, and a sunshade net with the shading degree of 70 percent is added, the temperature in the shed is controlled to be 15 to 35 ℃, and the air humidity is controlled to be 70 to 90 percent.
Optionally, the dendrobium officinale tissue culture bottle is placed in a greenhouse for culturing for 2 weeks, then the cover is removed, the dendrobium officinale tissue culture bottle is cultured for 1 week, and then the dendrobium officinale tissue culture seedling is taken out and cleaned.
Optionally, the dendrobium officinale tissue culture seedlings are in a hardening and planting mode that the roots of the dendrobium officinale tissue culture seedlings are upward and the stem ends of the dendrobium officinale tissue culture seedlings are downward on the vertical support frame, and the interval between every two dendrobium officinale tissue culture seedlings is 8-10 cm.
Optionally, the distance between the bottom vertical surface of the fir tree piece and the ground is more than 30cm, so that the dendrobium officinale tissue culture seedling planted on the fir tree piece is prevented from hanging down and contacting the ground after growing.
Alternatively, the nail used in the nail gun may be, but is not limited to, a stainless steel staple having a width of 12mm, a length of 10mm, and a thickness of 0.6mm.
Alternatively, the nonwoven fabric wrapping paper may have a length of 3cm and a width of 2cm after wrapping.
Optionally, 500W water pump that facade spraying railcar loaded, both saved the electric quantity and can effectively spraying water and liquid fertilizer again.
The three-dimensional cultivation method for the greenhouse dendrobium officinale tissue culture seedlings solves the practical problems of low cultivation space utilization rate, low seedling hardening survival rate, high manual management difficulty and serious water and fertilizer waste in the industrialized three-dimensional cultivation of the greenhouse dendrobium officinale tissue culture seedlings, and has a far-reaching market popularization prospect.
In some specific embodiments, 6 sections of fir wood with the diameter of 1dm and the length of 1.2m are selected, and 6 fir bark pieces with the length of 1.2m, the width of 0.5m and the thickness of 0.5cm are manufactured by using a round wood peeling machine. A vertical support frame with the length of 1.18m, the height of 2m and the thickness of 2cm is customized, the vertical support frame is a movable hollow galvanized pipe frame with a base, the vertical support frame is installed in a greenhouse, 6 fir barks face outwards, and each vertical face is fixed on the vertical support frame through a large iron clamp in a mode of 3 fir barks, so that a seedling planting area with double-sided fir barks and the planting area with the width of 1.2 meters and the height of 2 meters is formed. The vertical surface supporting frames are connected in front and back in the greenhouse and are arranged in a straight line to form a double-sided fir bark vertical surface wall with the length of more than 15 meters, and each opposite vertical surface supporting frame is provided with a passageway with the width of 1 meter.
Selecting dendrobium officinale tissue culture bottle seedlings containing 5 true leaves and part of roots of which are 3cm long, moving the seedlings out of a bottle for cleaning after the tissue culture seedlings adapt to the environment, then airing the tissue culture seedlings on a seedbed until the root base parts are whitish, and then flattening the dendrobium officinale tissue culture seedlings on the bark of the Chinese fir in a manner of 3 plants, 12 clusters, 1 rows, 1 first vertical surface and 1 second vertical surface. Packaging the pine bark powder into non-woven fabric packaging paper by using an automatic packaging machine, and then placing the packaging paper on the roots of the dendrobium officinale tissue culture seedlings. Preparing palm fibers or palm ropes, fixing one end of a strip at the left end of the vertical face of the cedar by using a nail gun, pulling the left end to the right end, simultaneously binding and pressing the root of the tissue culture seedling of the dendrobium officinale, the wrapping paper and the palm band on the root face support frame by using the nail gun in sequence, starting hardening the seedling, or directly pressing the root of the tissue culture seedling on the vertical face support frame by using the palm band without placing the wrapping paper containing pine bark powder, starting hardening the seedling, and then applying 1 g/L of pine bark extract solution to the root of the tissue culture seedling of the dendrobium officinale once a week. After 30 days of vertical face hardening off period, new root systems can be established on the vertical face support frame by the dendrobium officinale tissue culture seedlings, the stem leaves grow normally, after 90 days, vertical face planting of most dendrobium stems is completed, thickening and growth of stems are obvious, the leaves are thickened and the color is deepened. The steps are vertical surface hardening and planting of the dendrobium officinale tissue culture seedling.
When the vertical surface of the dendrobium officinale tissue culture seedling is used for hardening and planting, the automatic water-fertilizer spraying device for the industrialized vertical surface cultivation of the dendrobium officinale tissue culture seedling is arranged at the same time. Laying a first track and a second track on a passageway between two opposite vertical face support frames, arranging a spraying rail car with the programmable length of 0.8m and the width of 0.5m on the tracks to walk, placing a liquid storage tank with the length of 60cm, the width of 48cm and the height of 70cm at the tail part of the spraying rail car, respectively erecting a vertical rod with the height of 1.8m at the left and right positions of the spraying rail car, respectively and uniformly arranging 5 spray heads on the vertical rods, wherein the spray heads face the vertical face support frames, are connected in series by high-pressure explosion-proof water pipes and then are connected onto a water pump, and a water inlet pipe of the water pump is arranged at the bottom of the liquid storage tank. Water and liquid fertilizer need pour into and deposit in the liquid storage pot, in time adjust the liquid manure volume in the liquid storage pot through water level control equipment before the spraying, after the spraying railcar programming, with the speed of 0.5 meter/second along first track and second track operation when beginning to spray water or liquid fertilizer, start the water pump simultaneously with the even spraying of water and liquid fertilizer in the liquid storage pot to the dendrobium officinale tissue culture seedling of facade support frame cultivation, finish the spraying after making a round trip once along first track and second track, 1 day morning and night respectively 1 time in summer when watering, 3 times in the middle of a week in winter, 1 day in evening 1 time in other times, once a month when fertilizing.
In another embodiment, a three-dimensional cultivation system for dendrobium officinale is applied to the three-dimensional cultivation method, and comprises a plurality of vertical surface support frames, a first rail, a second rail and a vertical surface spraying device, wherein each vertical surface support frame is provided with a first vertical surface and a second vertical surface, each first vertical surface and each second vertical surface are provided with a seedling planting area composed of barks, each first rail and each second rail are arranged between two opposite vertical surface support frames, each vertical surface spraying device is slidably arranged on each first rail and each second rail, and each vertical surface spraying device is used for spraying water and liquid fertilizer to the dendrobium officinale tissue culture seedlings on each vertical surface support frame. Wherein the distance between two opposite vertical surface support frames is 1.8-2 m.
Generally in big-arch shelter entablature department installation spraying device among the prior art, sprinkle and fertilize from dendrobium officinale tissue culture seedling top, nevertheless because dendrobium officinale tissue culture seedling is the tiling on the facade support frame, it is that dendrobium officinale tissue culture seedling is along vertical direction from the top down tiling promptly, spraying device among the prior art also sprays from the top of dendrobium officinale tissue culture seedling, and the coverage area that leads to spraying dendrobium officinale tissue culture seedling is little, and sprays inhomogeneously. For example, the dendrobium officinale tissue culture seedlings positioned in the uppermost row are easier to spray, and the dendrobium officinale tissue culture seedlings positioned in the lowermost row are not easy to spray.
In the invention, the first rail and the second rail are arranged between the two opposite vertical surface supporting frames, the spraying device is arranged on the first rail and the second rail in a sliding manner, the spraying device moves along the first rail and the second rail while spraying, and the spraying device is used for spraying the dendrobium officinale, so that large-area coverage and uniform spraying can be realized, and water resources can be saved. In addition, in traditional seedbed cultivation, if the watering volume is little, moisture can not permeate into the culture medium, and the root of the dendrobium officinale tissue culture seedling can not absorb moisture, if the watering volume is large, accumulated water is easy to form, and the dendrobium officinale tissue culture seedling needs to be dredged, otherwise root rot is easy to cause, and the technical problem can be solved through a vertical spraying mode.
Specifically, facade spraying device still includes liquid storage pot, water pump, first pole setting and second pole setting, and first pole setting and second pole setting set up respectively in the both sides of liquid storage pot, and the liquid storage pot is used for storing water and liquid fertilizer, is provided with a plurality of shower nozzles in first pole setting and second pole setting, and the water pump communicates with a plurality of shower nozzles and liquid storage pot respectively, through setting up a plurality of shower nozzles, can more evenly spray water and liquid fertilizer on dendrobium officinale tissue culture seedling.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A large-scale three-dimensional cultivation method of dendrobium officinale tissue culture seedlings in a greenhouse is characterized by comprising the following steps:
the method comprises the following steps that a plurality of vertical face support frames are installed in a greenhouse in a large-scale mode, and each vertical face support frame is provided with a first vertical face and a second vertical face;
fixing bark on the first vertical surface and the second vertical surface in a large scale, wherein the surface of the bark is away from the first vertical surface and the second vertical surface;
flatly paving the dendrobium officinale tissue culture seedlings meeting the preset conditions on the barks;
packaging the pine bark powder into non-woven fabric packaging paper;
placing the non-woven fabric wrapping paper on the root of the dendrobium officinale tissue culture seedling;
and sprinkling water and applying fertilizer to the dendrobium officinale tissue culture seedlings by the vertical spraying device.
2. The three-dimensional cultivation method as claimed in claim 1, wherein the step of laying the dendrobium officinale tissue culture seedling meeting the preset condition on the bark comprises:
selecting dendrobium officinale tissue culture seedlings containing 5-7 true leaves and part of the dendrobium officinale tissue culture seedlings with root lengths of 3-4 cm;
cleaning the dendrobium officinale tissue culture seedlings;
spreading the cleaned dendrobium officinale tissue culture seedling on a seedbed until the root base of the dendrobium officinale tissue culture seedling is white;
and spreading the dendrobium officinale tissue culture seedlings with white root parts on the barks from the seedbed according to preset arrangement.
3. The stereoscopic cultivation method according to claim 2, wherein the predetermined arrangement is that 3-4 dendrobium officinale tissue culture seedlings are in one cluster, 12-14 clusters of the dendrobium officinale tissue culture seedlings are in 1 row, and 6-8 rows of the dendrobium officinale tissue culture seedlings are arranged on the first vertical surface or the second vertical surface.
4. The stereoscopic cultivation method as claimed in claim 3, wherein the dendrobium officinale tissue culture seedling meeting the preset conditions and the non-woven fabric containing pine bark powder are fixed on the bark through palm fibers or palm ropes.
5. The stereoscopic cultivation method according to claim 1, wherein the step of providing a spraying device for spraying water and applying fertilizer to the dendrobium officinale tissue culture seedlings comprises:
a first rail and a second rail which are opposite to each other are laid between the two vertical face support frames;
a vertical face spraying rail car is arranged on the first rail and the second rail in a sliding mode;
a liquid storage tank for storing water and liquid fertilizer is arranged on the spraying rail car;
and driving the vertical surface spraying rail car to move on the first rail and the second rail, and spraying water and liquid fertilizer in the liquid storage tank to the dendrobium officinale tissue culture seedlings on the vertical surface support frame.
6. The method of claim 1, wherein the step of fixing the bark on the first and second vertical surfaces further comprises:
and (3) steaming and boiling the barks in a steam sterilizer for 30-40 minutes.
7. The method of claim 1, wherein the bark has a length of 1 to 1.4m, a width of 0.4 to 0.6m, and a thickness of 0.4 to 0.6cm.
8. The method of claim 1, wherein the bark is a whole bark of the adult fir tree that is directly peeled from the wood of the adult fir tree.
9. A scale three-dimensional cultivation system of a greenhouse dendrobium officinale tissue culture seedling is applied to the three-dimensional cultivation method of any one of claims 1 to 8, and is characterized by comprising the following steps:
the vertical surface support frames are provided with a first vertical surface and a second vertical surface, and the first vertical surface and the second vertical surface are provided with seedling planting areas composed of barks;
the first rail and the second rail are arranged between the two opposite vertical surface supporting frames; and
and the spraying device is arranged on the first rail and the second rail in a sliding manner, and is used for spraying water and liquid fertilizer to the dendrobium officinale tissue culture seedlings on the vertical surface support frame.
10. The stereoscopic cultivation system as claimed in claim 1, wherein the distance between two opposite vertical support frames is 1.8m to 2m.
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