CN104871968A - Method for propagating and planting dendrobium officinale - Google Patents
Method for propagating and planting dendrobium officinale Download PDFInfo
- Publication number
- CN104871968A CN104871968A CN201510216282.3A CN201510216282A CN104871968A CN 104871968 A CN104871968 A CN 104871968A CN 201510216282 A CN201510216282 A CN 201510216282A CN 104871968 A CN104871968 A CN 104871968A
- Authority
- CN
- China
- Prior art keywords
- dendrobium candidum
- water
- solution
- dendrobium
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y02P60/216—
Abstract
The invention discloses a method for propagating and planting dendrobium officinale. The propagating process comprises the following steps: collecting fruits of dendrobium officinale, firstly putting the fruits into KMnO4 with the weight concentration being 1%, soaking, taking out, cutting into broken particles by scissors, putting the particles into a culture bottle filled with a culture medium in advance, culturing for 10-15 days, separating the cultured particles into a plurality of culture bottles when seedlings grow up, and then separating the particles into bottles when the particles are cultured to grow to 1cm high with a plurality of seedlings in each bottle, and transplanting into the medium of a greenhouse when growing to 5-8cm high, wherein the medium of the greenhouse for transplanting is pine bark and pine tree saw dust. The planting method comprises the steps of transplanting, fertilizing management and preventing and treating plant diseases and insect pests. The dendrobium officinale obtained by the propagating and planting method can grow rapidly, has few plant diseases and insect pests and is good in quality.
Description
Technical field
The present invention relates to breeding and implantation methods of a kind of implantation methods of traditional Chinese medicine, particularly dendrobium candidum.
Background technology
Dendrobium candidum (Dendrobium candidum wall.ex Lindl.) is the orchid family (Orchidaeae) Dendrobium (Dendrobium) herbaceos perennial; it is China's rare Chinese medicine; it is Chinese Second Class Key Protected Plant; have the laudatory title of " the celestial grass of China ", " in medicine gold "; first of row " Chinese nine immortal grass ", be called " medicine circle giant panda " by international medicinal plant circle.The effect such as there is immunological regulation, delay senility.Be not only the required raw material of familiar " MAILUONING ZHUSHEYE ", or the necessary raw material of tens of kinds of Chinese patent drugs and health products.
In Shennong's Herbal, dendrobium candidum is listed in top grade, has nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid, and the effect such as moisten the lung and relieve the cough.Modern pharmacological research shows, dendrobium candidum also has develop immunitypty, anti-ageing, anticancer, antifatigue, the effect of hemangiectasis.Dendrobium candidum is mainly distributed in the tropical and subtropical region such as China, Thailand, Burma, Vietnam, domestic with Guangxi, Yunnan, Deng Weiyuan main producing region, Guizhou.Because human lives's scope constantly expands, add that long-term transition is plucked, existing wild resource is on the verge of exhaustion, and purchase volume significantly declines.China has forbidden felling for many years, and the market price is significantly raised up.Dendrobium candidum is difficult to meet the need of market because wild resource is exhausted, therefore needs a large amount of artificial cultivation to meet the market demand constantly expanded.
Wild Dendrodium growth is from life in warm, moistening, few illumination, suitably ventilative, root system is breathed freely, draining is good environment, and need to adhere to trees, precipice seam carries out climbing raw or hanging growth, and the growth of iron sheet is quite harsh to the requirement of environment.Some scientific research institutions of China and business unit constantly explore the group training of orchid dendrobium candidum and artificial cultivation technique and test, achieve certain achievement, but remain at dendrobium candidum transplanting survival rate low, growth rate is slow, the subject matters such as output is not high, and active constituent content is low.
People have studied the growth conditions of dendrobium candidum in recent years, and various places plantation dendrobium candidum is greatly developed, and the document of research candidum tissue culturing and plantation also has, such as:
1. the method for a dendrobium candidum tissue cultivation forming seedling through one step culture, application number: CN201210368914.4, open (bulletin) number: CN102845308A, open (bulletin) day: 2013.01.02, application (patent right) people: Zhejiang Research Institute of Traditional Chinese Medicine Co., Ltd., summary: a kind of dendrobium candidum tissue cultivates the method for forming seedling through one step culture, the method passes through ratio and the dosage of two kinds of hormones in rational allocation medium, add the auxiliary materials such as active carbon simultaneously, Seeds of Dendrobium Candidum direct development is made to become seedling, after seedling being transferred to strong seedling culture base, survival rate more than 99.5%.The seedling time shorten that dendrobium candidum tissue can be cultivated by the present invention 2 months, significantly improves culture efficiency, reduces Financial cost, can be used as the important method that candidum tissue culturing seedling is produced.
2. the seed tissue culture propagation method of dendrobium candidum, application number: CN201310282961.1, open (bulletin) number: CN103314858A, open (bulletin) day: 2013.09.25, application (patent right) people: Chongqing City Xiushan Redstar TCM Herbals Development Co., Ltd. makes a summary: the seed tissue culture propagation method of dendrobium candidum, belong to traditional Chinese medicine planting technology field, comprise and introduce a fine variety sterilizing, Fiber differentiation, differentiation cultivation, strong sprout and the large step of culture of rootage four; Its beneficial effect adopts seed group to train the seedling obtained, and not easily degenerates, hereditary dimensionally stable, and active principle content is close with wild varieties, ensure that the quality of seed; The seed amount of dendrobium candidum is large, greatly can increase the reproductive number of seed; The inventive method also substantially reduces the tissue culture propagation cycle of dendrobium candidum simultaneously, only needing 4 ~ 5 months, substantially reducing the production time, reducing production cost from shearing the group training cycle of seed to seedling of taking root.
3. the method application number of a non-polluted planting dendrobium candidum: CN201410217988.7, open (bulletin) number: CN103999672A, open (bulletin) day: 2014.08.27, application (patent right) people: Puer City Institute of National Tradition Pharmaceutical makes a summary: a kind of method of non-polluted planting dendrobium candidum.Solve dendrobium candidum implantation methods, a kind of method of non-polluted planting dendrobium candidum, comprise the following steps: 1. plantlet in vitro breeding; 2. place election; 3. ground cultivation under booth; 4. matrix is laid; 5. planting seedlings; 6. daily planting environment; 7. daily management; 8. to gather preservation.Plantlet in vitro transplant after survival rate high, growth is fast, output is high, nutrition close to dendrobium officinale, medical value is high, Pesticide use is few, damage by disease and insect occurs few.
4. dendrobium candidum capsule rapid seedling cultivation method, application number: CN200910094673.7, open (bulletin) number: CN101933456A, open (bulletin) day: 2011.01.05, application (patent right) people: Xishuangbanna Zengliang Biotechnology Co., Ltd. makes a summary: a kind of rapid seedling cultivation method of Precious, Rare, Endangered traditional Chinese medicine dendrobium candidum capsule, under the prerequisite not destroying wild Dendrodium resource, fast-propagation high-quality seedlings of Dendrobium officinale.A dendrobium candidum capsule can go out the above dendrobe tissue culture seedling of 100,000 strain by quick propagating and cultivating by the group training cultivation in 5 ~ June, and acclimatization and transplants survival rate is to more than 96%.The present invention improves reproductive speed and the output of seedlings of Dendrobium officinale, and becoming wild be family's kind, implement artificial commerial growing, regenerates and sustainable utilization has very active influence to resources of medicinal plant in short supply in imminent danger.
Above-mentioned open source literature gives the group training of some dendrobium candidums and the method for plantation dendrobium candidum, make the artificial cultivation of dendrobium candidum can obtain a fairly large number of dendrobium candidum product, instead of the situation of plucking natural dendrobium candidum, but, disclosed dendrobium candidum document also has some defects, and the artificial dendrobium candidum quality that cultivation obtains is good not as natural dendrobium candidum.
Summary of the invention
The present invention, according to the growing environment of dendrobium candidum and feature, provides applicable dendrobium candidum to grow fast, superior in quality group training and the method for plantation dendrobium candidum.
The object of the invention is to be achieved through the following technical solutions.
1, the group training process of dendrobium candidum
Gather the fruit of dendrobium candidum, the preferably fruit of the dendrobium officinale of quality better, prematurity and maturation can, be first put into the KMnO of weight concentration 1%
4soak 1 minute, take out, be cut into particle with scissors, more broken better, but can not grind, be put into and be equipped with in the blake bottle of medium in advance, cultivate 10-15 days, see that seedling grows, be divided into several blake bottles, cultivate and grow to 1 centimetre high later sub-bottle again, every bottle is tens seedling, grows to 5-8 centimetre high and can be transplanted in the matrix of booth.
Described medium is potato and banana is primary raw material, adds macroelement, trace element, organic nutrient substance, growth hormone and water and makes.Its proportioning is as follows:
Below describe process for preparation:
1, prepare macroelement, in often liter of pure water, add following raw material:
Potassium nitrate 18-20; Ammonium nitrate 15-18; Potassium dihydrogen phosphate 1-2; Magnesium sulfate 3-5; Calcium chloride 6-10; Ferrous sulfate 2-4;
2, preparation trace element, adds following raw material in often liter of pure water:
Potassium iodide 0.05-0.10; Boric acid 0.20-0,80; Manganese sulphate 1.00-2.00; Zinc sulphate 1.00-2.00; Sodium molybdate 0.01-0,04; Copper sulphate 0.002-0.005; Cobalt chloride (6 water) 0.002-0.003;
3, prepare organic nutrient substance, in often liter of pure water, add following raw material,
Inositol 9-12; Lysine 1-2; Glycine 0.1-0.3; Vitamin B1 0.010.02; Vitamin B6 0.05-0.01;
4, prepare growth hormone, in often liter of pure water, add following raw material growth hormone NAA(IBA): 500mg+500ml water+2% sodium hydrate aqueous solution obtains the aqueous solution of 1mg/1ml after dissolving, be equivalent to 1ml solution containing hormone 1mg;
5, prepare basic element of cell division 6BA, add the aqueous solution obtaining 1mg/1ml after basic element of cell division 6BA:500mg+500ml water+2% aqueous hydrochloric acid solution dissolves in often liter of pure water, be equivalent to 1ml solution containing hormone 1mg;
Medium process for preparation is as follows:
1, get ripe banana 150g, the potato of boiling 100 grams, add distilled water making beating evenly, then constant volume is to 1L; Obtain solution A;
2, take 15 grams, brown sugar 60g, agar, put into 2 liters of containers, add macroelement: 100m1; Organic nutrient substance: 20ml; Trace element: 20m1; Growth hormone: 2ml; Basic element of cell division 6BA:2ml obtains solution B;
3, join in solution B by solution A, limit edged stirs;
4, by above-mentioned solution watery hydrochloric acid or diluted sodium hydroxide solution adjust ph to 6.1-6.5;
5,5-10 minute is boiled in heating; Calling in while hot in tissue culture bottle, is approximately highly 2.5-3.0cm;
6, build lid to tighten, put into pressure cooker sterilizing;
7, after sterilizing, take out and be placed on shelf, allow it naturally cool, period does not rock, in order to avoid agar does not solidify;
8, within after taking the dish out of the pot at least 24 hours, just can inoculate later, cut off from length some when preferably placing, can find out that whether medium is contaminated.
The implantation methods process of dendrobium candidum of the present invention comprises the control of transplanting, fertilizing management and damage by disease and insect.
The matrix of described transplanting is pine bark and pine tree sawdust, wherein pine bark and pine tree sawdust weight ratio are 1:1, need the particle smashing 1-5mm size, 10 minutes desinsections are steamed with steam, carry out disinfection with potassium permanganate solution again, concentration is that per kilogram water adds 0.2-0.5 gram, soaks sterilizing in 10-20 hour, then with spring or purified rinse water clean.
In order to prevent and treat butt rot, shifted out by the dendrobium candidum of 5-8 centimetre high before transplanting from blake bottle, the carbendazim with 50% dilutes 800 times and soaks 10 minutes, can be transplanted on implant bed.
Implant bed is placed on the plastic plate of perforate by plantation dendrobium officinale substrate, and stromal thickness is not less than 8 centimetres; Be clean cobblestone or lime stone below plastic plate, particle diameter 2-5 centimetre, piecemeal is planted, and pavement is left in centre.
Set up net canopy above implant bed, summer takes sun-proof, has warming facility winter.
Described fertilizing management; Growth of Dendrobium candidum needs fertilising, and the fertilizer that the present invention executes as required is divided into solid fertilizer and Liquid Fertilizer, and the weight content of the raw material of solid fertilizer is: fermentation cow dung 20-30 part; Pine needle meal 10-15 part; Brown sugar 3-5 part; Bone meal 5-10; Potassium dihydrogen phosphate 10-20; Ferrous sulfate 1-2 part; Solid fertilizer will make particle, will lead to 105-110 DEG C of steam and carry out disinfection, then pack with plastic bag sealing before making particle, if likely, particle outer wrapping one deck water-soluble resin makes slow-release fertilizer.
Matrix of the present invention and fertilizer all contain pine tree material: pine bark, pine tree sawdust and pine needle meal, and they contain rosin, can prevent some damage by disease and insect, also have soft, ventilative characteristic.
Described Liquid Fertilizer is peanut press pulp zymotic fluid, and it is that 1000 kilograms of spring add 1 kilogram of peanut press pulp, adds 100 grams, brown sugar, is put into closed container and ferments more than 5 months.
Solid fertilizer was respectively executed once in summer in spring and autumn, and fertilising in the every 20-30 of Liquid Fertilizer days once.
Using method, around matrix solid fertilizer being put into plantation dendrobium candidum plant, do not press close to plant dendrobium candidum plant, slowly dissolve under being allowed to condition at natural environment, absorb to dendrobium candidum, generally according to the growing state of dendrobium candidum, every square metre of dendrobium candidum planting tray adds 0.05-0.1 kilogram.Liquid Fertilizer fertilising can adopt drip irrigation method.
The control of described damage by disease and insect: (l) stem of noble dendrobium black spot cause harm blade make blade wither, the 3-5 month occurs.Prevention and controls: the carbendazim l000 times of liquid spraying of available 50% l-2 time.(2) stem of noble dendrobium anthracnose causes harm blade and stem branch, and brown or black scab appear in blade of being injured, and 1-5 is monthly generation.Prevention and controls: spray 2-3 time with 50% carbendazim l000 times liquid or 50% thiophanate methyl, 1000 times of liquid.(3) stem of noble dendrobium armored scale parasitizes plant leaf edge or the back side, sucks juice, and late May is the hatching Sheng phase.Prevention and controls: with the insecticide spraying of oneself preparation, raw material and the parts by weight of insecticide are as follows: garlic 20 ~ 30 parts, polygonum flaccidum 20 ~ 30 parts, fruit 1 ~ 2 part of washing one's hands, smashes above-mentioned material, add 5 ~ 10 times of water, half an hour is boiled in heating, and get filtrate, often liter of filtrate adds terpineol 5 ~ 10ml again, thin up 100-300 doubly, is sprayed onto damaged by vermin place.
Dendrobium candidum of the present invention grows to can be gathered in the crops for more than 2 years or 20 centimetres.
Advantage of the present invention:
1, the fruit gathering the dendrobium officinale of quality better carries out group training and breeds, and the kind of the dendrobium candidum obtained is excellent.
2, the medium of oneself preparation is effective, emerges neat.
The preparation of the matrix of 3, transplanting adopts loose warp to cross bark and the pine tree sawdust of strict sterilization, and after transplanting, dendrobium candidum damage by disease and insect is few.
4, the solid fertilizer prepared and Liquid Fertilizer are applicable to Growth of Dendrobium candidum needs.
5, the control of damage by disease and insect can prevent and treat stem of noble dendrobium black spot, stem of noble dendrobium anthracnose and stem of noble dendrobium armored scale etc. effectively, ensures Growth of Dendrobium candidum.
6, fast growth, active chemical content is high.
Embodiment
Embodiment 1
The present inventor carries out breeding of dendrobium candidum in Qin Tang town, Guigang, Guangxi and plants,
Group training process is as follows:
Pluck the immature Dendrobium officinale fruit that Guangxi Mountains is wild, be first put into the KMnO of weight concentration 1%
4soak 1 minute, take out, particle is cut into scissors, be put into and be equipped with in the blake bottle of medium in advance, cultivate 10-15 days, see that seedling grows, be divided into several blake bottles, cultivate and grow to 1 centimetre high later sub-bottle again, every bottle is tens seedling, grows to 5-8 centimetre high and can be transplanted in the matrix of booth;
Described medium process for preparation is as follows
(1) prepare macroelement, in often liter of pure water, add following raw material:
Potassium nitrate 18-20; Ammonium nitrate 15-18; Potassium dihydrogen phosphate 1-2; Magnesium sulfate 3-5; Calcium chloride 6-10; Ferrous sulfate 2-4;
(2) preparation trace element, adds following raw material in often liter of pure water:
Potassium iodide 0.05-0.10; Boric acid 0.20-0,80; Manganese sulphate 1.00-2.00; Zinc sulphate 1.00-2.00; Sodium molybdate 0.01-0,04; Copper sulphate 0.002-0.005; Cobalt chloride (6 water) 0.002-0.003;
(3) prepare organic nutrient substance, in often liter of pure water, add following raw material;
Inositol 9-12; Lysine 1-2; Glycine 0.1-0.3; Vitamin B1 0.010.02; Vitamin B6 0.05-0.01;
(4) growth hormone is prepared, growth hormone NAA(IBA): 500mg+500ml water+2% sodium hydrate aqueous solution obtains the aqueous solution of 1mg/1ml after dissolving, be equivalent to 1ml solution containing hormone 1mg;
(5) prepare the aqueous solution obtaining 1mg/1ml after basic element of cell division 6BA:6BA500mg+500ml water+2% aqueous hydrochloric acid solution dissolves, be equivalent to 1ml solution containing hormone 1mg;
Medium process for preparation is as follows:
1, get ripe banana 150g, the potato of boiling 100 grams, add distilled water making beating evenly, then constant volume is to 1L; Obtain solution A;
2, take 15 grams, brown sugar 60g, agar, put into 2 liters of containers, add macroelement: 100m1; Organic organic nutrient substance: 20ml; Trace element: 20m1; Growth hormone: 2ml; The basic element of cell division: 2ml; Obtain solution B;
3, join in solution B by solution A, limit edged stirs;
4, by above-mentioned solution watery hydrochloric acid or diluted sodium hydroxide solution adjust ph to 6.1-6.5;
5,5-10 minute is boiled in heating; Calling in while hot in tissue culture bottle, is approximately highly 2.5-3.0cm;
6, build lid to tighten, put into pressure cooker sterilizing;
7, after sterilizing, take out and be placed on shelf, allow it naturally cool, period does not rock, in order to avoid agar does not solidify;
8, within after taking the dish out of the pot at least 24 hours, just can inoculate later, cut off from length some when preferably placing, can find out that whether medium is contaminated.
The preparation of the matrix of transplanting:
Get pine bark and pine tree sawdust, wherein pine bark and pine tree sawdust weight ratio are 1:1, smash the particle of 1-5mm size, 10 minutes desinsections are steamed with steam, carry out disinfection with potassium permanganate solution again, concentration is that per kilogram water adds 0.2-0.5 gram, soaks sterilizing in 10-20 hour, then with spring or purified rinse water clean.
In order to prevent and treat butt rot, shifted out by the dendrobium candidum of 5-8 centimetre high before transplanting from blake bottle, the carbendazim with 50% dilutes 800 times and soaks 10 minutes, can be transplanted on implant bed.
Implant bed is placed on the plastic plate of perforate by plantation dendrobium officinale substrate, and stromal thickness is not less than 8 centimetres; Be clean cobblestone or lime stone below plastic plate, particle diameter 2-5 centimetre, piecemeal is planted, and pavement is left in centre.
Set up net canopy above implant bed, summer takes sun-proof, has warming facility winter.
The making of solid fertilizer and Liquid Fertilizer:
The weight content of the raw material of solid fertilizer is: fermentation cow dung 20-30 part; Pine needle meal 10-15 part; Brown sugar 3-5 part; Bone meal 5-10; Potassium dihydrogen phosphate 10-20; Ferrous sulfate 1-2 part; Make particle, logical 105-110 DEG C of steam carries out disinfection, and then packs with plastic bag sealing.
Liquid Fertilizer is peanut press pulp zymotic fluid, and it is that 1000 kilograms of spring add 1 kilogram of peanut press pulp, adds 100 grams, brown sugar, is put into closed container and ferments more than 5 months.
Solid fertilizer was respectively executed once in summer in spring and autumn, and fertilising in the every 20-30 of Liquid Fertilizer days once.
Using method, around matrix solid fertilizer being put into plantation dendrobium candidum plant, do not press close to plant dendrobium candidum plant, slowly dissolve under being allowed to condition at natural environment, absorb to dendrobium candidum, generally according to the growing state of dendrobium candidum, every square metre of dendrobium candidum planting tray adds 0.05-0.1 kilogram.Liquid Fertilizer fertilising can adopt drip irrigation method.
The control of damage by disease and insect.
(l) stem of noble dendrobium black spot cause harm blade make blade wither, the 3-5 month occurs.Prevention and controls: the carbendazim l000 times of liquid spraying of available 50% l-2 time.
(2) stem of noble dendrobium anthracnose causes harm blade and stem branch, and brown or black scab appear in blade of being injured, and 1-5 is monthly generation.Prevention and controls: spray 2-3 time with 50% carbendazim l000 times liquid or 50% thiophanate methyl, 1000 times of liquid.
(3) stem of noble dendrobium armored scale parasitizes plant leaf edge or the back side, sucks juice, and late May is the hatching Sheng phase.Prevention and controls: with the insecticide spraying of oneself preparation, raw material and the parts by weight of insecticide are as follows: garlic 20 ~ 30 parts, polygonum flaccidum 20 ~ 30 parts, fruit 1 ~ 2 part of washing one's hands, smashes above-mentioned material, add 5 ~ 10 times of water, half an hour is boiled in heating, and get filtrate, often liter of filtrate adds terpineol 5 ~ 10ml again, thin up 100-300 doubly, is sprayed onto damaged by vermin place.
The dendrobium candidum of the training of embodiment of the present invention group and plantation has grown to 20 centimetres high for 18 months, and branch is full, and leaf is emerald green, by analysis, its dendrobium candidum element A, dendrobium candidum element B, the chemical composition of dendrobium candidum element C and wild close is higher than the dendrobium candidum content bought from Hunan company.
Claims (4)
1. the mating system of dendrobium candidum and implantation methods, is characterized in that: reproductive process is the fruit gathering dendrobium candidum, is first put into the KMnO of weight concentration 1%
4soak 1 minute, take out, particle is cut into scissors, be put into and be equipped with in the blake bottle of medium in advance, cultivate 10-15 days, see that seedling grows, be divided into several blake bottles, cultivate and grow to 1 centimetre high later sub-bottle again, every bottle is tens seedling, grows to 5-8 centimetre high and can be transplanted in the matrix of booth;
Described medium is potato and banana is primary raw material, and add macroelement, trace element, organic nutrient substance, growth hormone and water and make, its proportioning is as follows:
(1) prepare macroelement, in often liter of pure water, add following raw material:
Potassium nitrate 18-20; Ammonium nitrate 15-18; Potassium dihydrogen phosphate 1-2; Magnesium sulfate 3-5; Calcium chloride 6-10; Ferrous sulfate 2-4;
(2) preparation trace element, adds following raw material in often liter of pure water:
Potassium iodide 0.05-0.10; Boric acid 0.20-0,80; Manganese sulphate 1.00-2.00; Zinc sulphate 1.00-2.00; Sodium molybdate 0.01-0,04; Copper sulphate 0.002-0.005; Cobalt chloride (6 water) 0.002-0.003;
(3) prepare organic nutrient substance, in often liter of pure water, add following raw material,
Inositol 9-12; Lysine 1-2; Glycine 0.1-0.3; Vitamin B1 0.010.02; Vitamin B6 0.05-0.01;
(4) prepare growth hormone, in often liter of pure water, add growth hormone NAA(IBA): 500mg+500ml water+2% sodium hydrate aqueous solution obtains the aqueous solution of 1mg/1ml after dissolving, be equivalent to 1ml solution containing hormone 1mg;
(5) prepare in basic element of cell division 6BA: often liter pure water the aqueous solution adding and obtain 1mg/1ml after basic element of cell division 6BA:500mg+500ml water+2% aqueous hydrochloric acid solution dissolves, be equivalent to 1ml solution containing hormone 1mg;
Described medium process for preparation is as follows:
(1) get ripe banana 150g, the potato of boiling 100 grams, add distilled water making beating evenly, then constant volume is to 1L; Obtain solution A;
(2) take 15 grams, brown sugar 60g, agar, put into 2 liters of containers, add macroelement: 100m1; Organic nutrient substance: 20ml; Trace element: 20m1; Growth hormone: 2ml; Basic element of cell division 6BA:2ml obtains solution B;
(3) join in solution B by solution A, limit edged stirs;
(4) by above-mentioned solution watery hydrochloric acid or diluted sodium hydroxide solution adjust ph to 6.1-6.5;
(5) 5-10 minute is boiled in heating; Calling in while hot in tissue culture bottle, is approximately highly 2.5-3.0cm;
(6) build lid to tighten, put into pressure cooker sterilizing;
(7), after sterilizing, take out and be placed on shelf, allow it naturally cool, period does not rock, in order to avoid agar does not solidify;
(8) within after taking the dish out of the pot at least 24 hours, just can inoculate later, cut off from length some when preferably placing, can find out that whether medium is contaminated.
2. the mating system of dendrobium candidum according to claim 1 and implantation methods, it is characterized in that: the booth matrix of described transplanting is pine bark and pine tree sawdust, wherein pine bark and pine tree sawdust weight ratio are 1:1, smash the particle of 1-5mm size, steam 10 minutes desinsections with steam, then carry out disinfection with potassium permanganate solution, concentration is that per kilogram water adds 0.2-0.5 gram, soak sterilizing in 10-20 hour, then with spring or purified rinse water clean.
3. the mating system of dendrobium candidum according to claim 1 and implantation methods, is characterized in that: the implantation methods process of dendrobium candidum comprises the control of transplanting, fertilizing management and damage by disease and insect
;
(1) transplanting, in order to prevent and treat butt rot, being shifted out by the dendrobium candidum of 5-8 centimetre high before transplanting from blake bottle, the carbendazim with 50% dilutes 800 times and soaks 10 minutes, can be transplanted on the implant bed of dendrobium officinale substrate;
Set up net canopy above implant bed, summer takes sun-proof, has warming facility winter;
(2) fertilizing management, described fertilizer is divided into solid fertilizer and Liquid Fertilizer;
The weight content of the raw material of described solid fertilizer is: fermentation cow dung 20-30 part; Pine needle meal 10-15 part; Brown sugar 3-5 part; Bone meal 5-10; Potassium dihydrogen phosphate 10-20; Ferrous sulfate 1-2 part; Solid fertilizer will make particle, will lead to 105-110 DEG C of steam and carry out disinfection, then pack with plastic bag sealing before making particle, or particle outer wrapping one deck water-soluble resin makes slow-release fertilizer;
Described Liquid Fertilizer is peanut press pulp zymotic fluid, and it is that 1000 kilograms of spring add 1 kilogram of peanut press pulp, adds 100 grams, brown sugar, is put into closed container and ferments more than 5 months;
Solid fertilizer was respectively executed once in summer in spring and autumn, and fertilising in the every 20-30 of Liquid Fertilizer days once;
(3) control of damage by disease and insect;
Stem of noble dendrobium black spot blade of causing harm makes blade wither, and the 3-5 month occurs, prevention and controls: carbendazim l000 times of liquid of available 50% is sprayed l-2 time;
Stem of noble dendrobium anthracnose causes harm blade and stem branch, and brown or black scab appear in blade of being injured, and 1-5 is monthly generation; Prevention and controls: spray 2-3 time with 50% carbendazim l000 times liquid or 50% thiophanate methyl, 1000 times of liquid;
Stem of noble dendrobium armored scale parasitizes plant leaf edge or the back side, sucks juice, and late May is the hatching Sheng phase; Prevention and controls: with the insecticide spraying of oneself preparation, raw material and the parts by weight of insecticide are as follows: garlic 20 ~ 30 parts, polygonum flaccidum 20 ~ 30 parts, fruit 1 ~ 2 part of washing one's hands, smashes above-mentioned material, add 5 ~ 10 times of water, half an hour is boiled in heating, and get filtrate, often liter of filtrate adds terpineol 5 ~ 10ml again, thin up 100-300 doubly, is sprayed onto damaged by vermin place.
4. the mating system of dendrobium candidum according to claim 3 and implantation methods, is characterized in that: implant bed is placed on the plastic plate of perforate by plantation dendrobium officinale substrate, and stromal thickness is not less than 8 centimetres; Be clean cobblestone or lime stone below plastic plate, particle diameter 2-5 centimetre, piecemeal is planted, and pavement is left in centre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510216282.3A CN104871968B (en) | 2015-05-03 | 2015-05-03 | Dendrobium candidum breed and implantation methods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510216282.3A CN104871968B (en) | 2015-05-03 | 2015-05-03 | Dendrobium candidum breed and implantation methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104871968A true CN104871968A (en) | 2015-09-02 |
| CN104871968B CN104871968B (en) | 2018-01-16 |
Family
ID=53939875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510216282.3A Expired - Fee Related CN104871968B (en) | 2015-05-03 | 2015-05-03 | Dendrobium candidum breed and implantation methods |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104871968B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218174A (en) * | 2015-10-21 | 2016-01-06 | 瑞丽市岭瑞农业开发有限公司 | For the liquefaction fertilizer making method of Herba Dendrobii root |
| CN105638410A (en) * | 2016-01-05 | 2016-06-08 | 安徽霍山县草之灵中药材有限公司 | Dendrobium mountain planting method |
| CN105724213A (en) * | 2016-02-27 | 2016-07-06 | 云南农业大学 | Formula of medium for planting dendrobium officinale |
| CN105794615A (en) * | 2016-03-31 | 2016-07-27 | 唐云龙 | Planting method for dendrobium officinale |
| CN107567937A (en) * | 2017-09-20 | 2018-01-12 | 贵州苗贵客贸易发展有限公司 | A kind of grape cuttage and seedling culture method |
| CN107602216A (en) * | 2017-09-11 | 2018-01-19 | 向群 | plant growth regulating base |
| CN108307985A (en) * | 2017-12-27 | 2018-07-24 | 康美药业（文山）药材种植管理有限公司 | A kind of Dendrobium Chrysotoxum Lindl culture substrate and preparation method |
| CN109769622A (en) * | 2017-11-10 | 2019-05-21 | 丹阳圣丽园农业发展有限公司 | A kind of antibacterial dendrobium candidum special cultivation soil |
| CN115443894A (en) * | 2022-09-15 | 2022-12-09 | 福建省亚热带植物研究所 | Large-scale three-dimensional cultivation method and cultivation system for dendrobium officinale tissue culture seedlings in greenhouse |
| CN116897832A (en) * | 2023-07-04 | 2023-10-20 | 云南农业大学 | One-step seedling culture method for dendrobium devonianum seeds |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845308A (en) * | 2012-09-27 | 2013-01-02 | 浙江省中药研究所有限公司 | Method for dendrobium officinale tissue culture one-step seedling |
| US8383881B2 (en) * | 2008-01-08 | 2013-02-26 | National University Of Kaohsiung | Method for producing polyploid plants of orchids |
| CN103314858A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Seed tissue culture propagation method of dendrobium candidum |
| CN103999672A (en) * | 2014-05-22 | 2014-08-27 | 普洱市民族传统医药研究所 | Pollution-free method for planting dendrobium nobile lindl |
-
2015
- 2015-05-03 CN CN201510216282.3A patent/CN104871968B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8383881B2 (en) * | 2008-01-08 | 2013-02-26 | National University Of Kaohsiung | Method for producing polyploid plants of orchids |
| CN102845308A (en) * | 2012-09-27 | 2013-01-02 | 浙江省中药研究所有限公司 | Method for dendrobium officinale tissue culture one-step seedling |
| CN103314858A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Seed tissue culture propagation method of dendrobium candidum |
| CN103999672A (en) * | 2014-05-22 | 2014-08-27 | 普洱市民族传统医药研究所 | Pollution-free method for planting dendrobium nobile lindl |
Non-Patent Citations (1)
| Title |
|---|
| 潘仕萍等: "云南珍稀植物铁皮石斛研究初报", 《林业调查规划》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218174A (en) * | 2015-10-21 | 2016-01-06 | 瑞丽市岭瑞农业开发有限公司 | For the liquefaction fertilizer making method of Herba Dendrobii root |
| CN105638410A (en) * | 2016-01-05 | 2016-06-08 | 安徽霍山县草之灵中药材有限公司 | Dendrobium mountain planting method |
| CN105724213A (en) * | 2016-02-27 | 2016-07-06 | 云南农业大学 | Formula of medium for planting dendrobium officinale |
| CN105794615A (en) * | 2016-03-31 | 2016-07-27 | 唐云龙 | Planting method for dendrobium officinale |
| CN107602216A (en) * | 2017-09-11 | 2018-01-19 | 向群 | plant growth regulating base |
| CN107602216B (en) * | 2017-09-11 | 2020-12-22 | 张芬 | Plant growth regulating base |
| CN107567937A (en) * | 2017-09-20 | 2018-01-12 | 贵州苗贵客贸易发展有限公司 | A kind of grape cuttage and seedling culture method |
| CN109769622A (en) * | 2017-11-10 | 2019-05-21 | 丹阳圣丽园农业发展有限公司 | A kind of antibacterial dendrobium candidum special cultivation soil |
| CN108307985A (en) * | 2017-12-27 | 2018-07-24 | 康美药业（文山）药材种植管理有限公司 | A kind of Dendrobium Chrysotoxum Lindl culture substrate and preparation method |
| CN115443894A (en) * | 2022-09-15 | 2022-12-09 | 福建省亚热带植物研究所 | Large-scale three-dimensional cultivation method and cultivation system for dendrobium officinale tissue culture seedlings in greenhouse |
| CN116897832A (en) * | 2023-07-04 | 2023-10-20 | 云南农业大学 | One-step seedling culture method for dendrobium devonianum seeds |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104871968B (en) | 2018-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104871968B (en) | Dendrobium candidum breed and implantation methods | |
| CN101336605B (en) | Dendrobium nobi tissue culturing and planting method | |
| CN106258483B (en) | Morchella multilayer base material planting method in high-cold high-altitude area | |
| CN102550252B (en) | Cultivation method of Dendrobium officinale | |
| CN103283450B (en) | Golden camellia bud seedling stock grafting propagation method | |
| CN104322257A (en) | Method for normatively planting dendrobium officinale in simple greenhouse | |
| CN102823401A (en) | Tie-to-tree dendrobium officinale cultivation method | |
| CN104541970B (en) | The method of northern area outdoor planting rhizoma Gastrodiae | |
| CN103004566A (en) | Planting method of Dendrobium officinale | |
| CN102523881A (en) | Industrial cultivation method for dendrobium candidum | |
| CN105104057B (en) | A kind of two sections of cultural methods of tribute mandarin orange plantation | |
| CN105594567A (en) | Planting method for Dendrobium officinale Kimura et Migo | |
| CN102960226B (en) | Dendrobium officinale Kimura et Migo tissue culture seedling acclimatization method | |
| CN106105795A (en) | A kind of method for culturing seedlings of oil tea | |
| CN103999750B (en) | A kind of propagation method of botrychiam ternatum spore | |
| CN106258484A (en) | A kind of high altitudes and cold area efficient implantation methods of Delicious lactarius | |
| CN107750854A (en) | A kind of cultural method of capsicum | |
| CN106986691A (en) | A kind of cultural method of spun gold emperor chrysanthemum | |
| CN106561428A (en) | Soilless culture method for pumpkins | |
| CN102285844A (en) | Preparation method of lilium spp nutrient fertilizer | |
| CN106212151A (en) | A kind of method for culturing seedlings of pearl guava | |
| CN104756690A (en) | Pesticide-free and fertilizer-free pure ecological Rhizoma Paridis high yield cultivation technology | |
| CN105075858A (en) | Liquid rapid-propagation method for rhizoma bletillae seeds | |
| CN103477862A (en) | Ecological culture method of calotropis gigantea plant | |
| CN108157052A (en) | The cultural method of blueberry tree |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180116 |