CN104871968B - Dendrobium candidum breed and implantation methods - Google Patents
Dendrobium candidum breed and implantation methods Download PDFInfo
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Abstract
The invention discloses a kind of mating system of dendrobium candidum and implantation methods, reproductive process is to gather the fruit of dendrobium candidum, is first put into the KMnO of weight concentration 1%4Immersion is taken out, and is cut into particle with scissors, is put into the blake bottle for being pre-loaded with culture medium, cultivate 10 15 days, it is seen that seedling grows, and is divided into several blake bottles, sub-bottle again is further cultured for growing to after 1 centimetre of height, every bottle is more than ten seedling, and growing to 58 centimetres of height can be transplanted in the matrix of greenhouse；The greenhouse matrix of the transplanting is pine bark and pine tree sawdust, and implantation methods process includes the preventing and treating of transplanting, fertilizing management and pest and disease damage, and the present invention breeds that the Growth of Dendrobium candidum speed obtained with implantation methods is fast, and pest and disease damage is few, quality better.
Description
Technical field
The present invention relates to a kind of implantation methods of Chinese medicine, particularly dendrobium candidum breed and implantation methods.
Background technology
Dendrobium candidum (Dendrobium candidum wall.ex Lindl.) is orchid family (Orchidaeae) stem of noble dendrobium
Belong to (Dendrobium) herbaceos perennial, be China's rare Chinese medicine, be Chinese Second Class Key Protected Plant, be known as " China's celestial being
Grass ", the laudatory title of " gold in medicine ", arrange first of " Chinese nine big celestial grass ", " medicine circle giant panda " is referred to as by international medicinal plant circle.Tool
There are immunological regulation, anti-aging and other effects.It is not only the required raw material of familiar " MAILUONING ZHUSHEYE ", or tens of kinds
The necessary raw material of Chinese patent drug and health products.
《Sheng Nong's herbal classic》In, dendrobium candidum is listed in top grade, has nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid, moistens the lung and relieve the cough
Effect.Modern pharmacological research shows that dendrobium candidum also has strengthen immunity, and anti-aging, anticancer is antifatigue, expands blood vessel
Effect.Dendrobium candidum is mainly distributed on the tropical and subtropical region such as China, Thailand, Burma, Vietnam, it is domestic with Guangxi, Yunnan,
Guizhou Deng Weiyuan main producing regions.Because human lives' scope constantly expands, plucked plus long-term transition, existing wild resource has been on the point of
Face exhaustion, purchase volume significantly declines.Forbid felling for many years in China so that the market price significantly raises up.Dendrobium candidum because
Wild resource is exhausted and is difficult to meet the market demand, it is therefore desirable to which a large amount of artificial cultivations are to meet the market needs constantly expanded.
Wild Dendrodium be grown in warm, moistening, few illumination, the appropriate environment that ventilative, root system is breathed freely, draining is good from
Raw precipice seam climb raw or hanging growth, it is necessary to adhere to trees, and the requirement of the growth of iron sheet to environment is quite harsh.China
Some scientific research institutions and business unit the tissue culture and artificial cultivation technique of orchid dendrobium candidum constantly explore and
Experiment, achieves certain achievement, but remains low in dendrobium candidum transplanting survival rate, and the speed of growth is slow, and yield is not high, effectively into
The subject matters such as point content is low.
People have studied the growth conditions of dendrobium candidum in recent years, and various regions plantation dendrobium candidum is greatly developed, studied
Candidum tissue culturing and the document of plantation also have, such as：
1. a kind of method of dendrobium candidum tissue culture forming seedling through one step culture, application number：CN201210368914.4, it is open (public
Accuse) number：CN102845308A, open (bulletin) day：2013.01.02, (patent right) people is applied for：Zhejiang Prov. Chinese Medicine Inst
Co., Ltd, summary：A kind of method of dendrobium candidum tissue culture forming seedling through one step culture, this method pass through in rational allocation culture medium two
The ratio and dosage of kind hormone, while add the auxiliary materials such as activated carbon so that Seeds of Dendrobium Candidum direct development is into seedling, by seedling
It is transferred to after strong seedling culture base, survival rate more than 99.5%.The present invention can contract the seedling time of dendrobium candidum tissue culture
It is short 2 months, culture efficiency is significantly improved, reduces financial cost, the important method that can be produced as candidum tissue culturing seedling.
2. the seed tissue culture propagation method of dendrobium candidum, application number：CN201310282961.1, open (bulletin) number：
CN103314858A, open (bulletin) day：2013.09.25, (patent right) people is applied for：The Red Star Chinese medicine exploitation of the Chongqing City Xiushan Mountain
Co., Ltd makes a summary：The seed tissue culture propagation method of dendrobium candidum, belong to Chinese medicine planting technology field, including introduce a fine variety sterilizing,
Fiber differentiation, differentiation culture, four big step of strong sprout and culture of rootage；Its advantage is to use the children obtained by seed tissue culture
Seedling, it is not easy to degenerate, hereditary dimensionally stable, active principle content is close with wild varieties, ensure that the quality of seed；Dendrobium candidum
Seed amount it is big, the reproductive number of seed can be greatly increased；The inventive method also substantially reduces dendrobium candidum simultaneously
In the tissue culture propagation cycle, only needed 4~5 months from shearing seed to the tissue culture cycle of rooted seedling, substantially reduce the production time, reduced
Production cost.
A kind of 3. method application number of non-polluted planting dendrobium candidum：CN201410217988.7, open (bulletin) number：
CN103999672A, open (bulletin) day：2014.08.27, (patent right) people is applied for：Puer City Institute of National Tradition Pharmaceutical
Summary：A kind of method of non-polluted planting dendrobium candidum.Solve dendrobium candidum implantation methods, a kind of non-polluted planting dendrobium candidum
Method, comprise the following steps：1. tissue-cultured seedling is bred；2. place election；3. ground is cultivated under greenhouse；4. lay matrix；5. plant
Seedling is colonized；6. daily planting environment；7. daily management；8. harvesting preserves.Survival rate is high after tissue culture transplantation of seedlings, growth is fast, yield
High, nutrition is close to dendrobium officinale, medical value is high, Pesticide use is few, pest and disease damage occurs less.
4. dendrobium candidum capsule rapid seedling cultivation method, application number：CN200910094673.7, open (bulletin) number：
CN101933456A, open (bulletin) day：2011.01.05, (patent right) people is applied for：It is limited that Xishuangbanna increases beautiful biotechnology
Company makes a summary：A kind of rapid seedling cultivation method of Precious, Rare, Endangered Chinese medicine dendrobium candidum capsule, is not destroying wild Dendrodium resource
Under the premise of, the high-quality seedlings of Dendrobium officinale of fast-propagation.One dendrobium candidum capsule can numerous training soon by the tissue culture culture in 5~June
Bring out more than 100,000 plants dendrobe tissue culture seedlings, acclimatization and transplantses survival rate to more than 96%.Present invention raising seedlings of Dendrobium officinale
Reproductive speed and output, become wild and implement artificial commerial growing into family's kind, to resources of medicinal plant in short supply regeneration in imminent danger
There is very active influence with sustainable utilization.
The method that document disclosed above gives the tissue culture of some dendrobium candidums and plants dendrobium candidum so that iron sheet stone
The artificial cultivation of dry measure used in former times can obtain a fairly large number of dendrobium candidum product, instead of the situation for plucking natural dendrobium candidum, but
It is that disclosed dendrobium candidum document also has some defects, the artificial dendrobium candidum quality for cultivating to obtain is not so good as natural iron sheet stone
Dry measure used in former times is good.
The content of the invention
Growing environment and feature of the invention according to dendrobium candidum, there is provided it is adapted to dendrobium candidum fast-growth, it is superior in quality
Tissue culture and plant dendrobium candidum method.
The purpose of the present invention is achieved by the following technical solution.
1st, the tissue culture process of dendrobium candidum
The fruit of the fruit, the preferably dendrobium officinale of quality better of dendrobium candidum is gathered, prematurity and maturation all may be used
To be first put into the KMnO of weight concentration 1%4Immersion 1 minute, take out, particle is cut into scissors, it is more broken better, but can not grind
Mill, is put into the blake bottle for being pre-loaded with culture medium, cultivates 10-15 days, it is seen that and seedling grows, and is divided into several blake bottles, then
Culture grows to after 1 centimetre of high sub-bottle again, and every bottle is more than ten seedling, and the matrix of greenhouse can be transplanted to by growing to 5-8 centimetres of height
In.
Described culture medium is potato and banana is primary raw material, adds macroelement, trace element, organic nutrition
Thing, auxin and water are made.Its proportioning is as follows：
Process for preparation described below：
1st, macroelement is prepared, following raw material is added in every liter of pure water：
Potassium nitrate 18-20；Ammonium nitrate 15-18；Potassium dihydrogen phosphate 1-2；Magnesium sulfate 3-5；Calcium chloride 6-10；Ferrous sulfate
2-4；
2nd, trace element is prepared, following raw material is added in every liter of pure water：
KI 0.05-0.10；Boric acid 0.20-0,80；Manganese sulfate 1.00-2.00；Zinc sulfate 1.00-2.00；Molybdic acid
Sodium 0.01-0,04；Copper sulphate 0.002-0.005；Cobalt chloride（6 water）0.002-0.003；
3rd, organic nutrient substance is prepared, following raw material is added in every liter of pure water,
Inositol 9-12；Lysine 1-2；Glycine 0.1-0.3；Vitamin B1 0.010.02；Vitamin B6 0.05-
0.01；
4th, auxin is prepared, following raw material auxin NAA is added in every liter of pure water（IBA）：500mg+500ml water+2%
The 1mg/1ml aqueous solution is obtained after sodium hydrate aqueous solution dissolving, equivalent to 1ml solution 1mg containing hormone；
5th, basic element of cell division 6BA is prepared, basic element of cell division 6BA is added in every liter of pure water：The hydrochloric acid of 500mg+500ml water+2%
The 1mg/1ml aqueous solution is obtained after aqueous dissolution, equivalent to 1ml solution 1mg containing hormone；
Culture medium process for preparation is as follows：
1st, the banana 150g of maturation is taken, 100 grams of cooked potato, adds distilled water mashing uniformly, then constant volume to 1L；
Obtain solution A；
2nd, 15 grams of brown sugar 60g, agar are weighed, is put into 2 liters of containers, adds macroelement：100m1；Organic nutrient substance：
20ml；Trace element：20m1；Auxin：2ml；Basic element of cell division 6BA：2ml obtains solution B；
3rd, solution A is added in solution B, it is stirring while adding uniform；
4th, above-mentioned solution watery hydrochloric acid or diluted sodium hydroxide solution are adjusted into pH value to 6.1-6.5；
5th, 5-10 minutes are boiled in heating；Call in while hot in tissue culture bottle, about height is 2.5-3.0cm；
6th, cover lid to tighten, be put into pressure cooker and sterilize；
7th, after sterilizing, taking-up is placed on shelf, allows its natural cooling, during which not rocked, in case agar does not solidify；
8th, it could be inoculated with after at least 24 hours after taking the dish out of the pot, length be cut off from when preferably placing a bit, it can be seen that culture medium
Whether it is contaminated.
The implantation methods process of dendrobium candidum of the present invention includes the preventing and treating of transplanting, fertilizing management and pest and disease damage.
The matrix of the transplanting is pine bark and pine tree sawdust, and wherein pine bark and pine tree sawdust weight ratio are 1:1, it is necessary to
The particle of 1-5mm sizes is smashed, steams 10 minutes desinsections with vapor, then is carried out disinfection with potassium permanganate solution, concentration is
Per kilogram water adds 0.2-0.5 grams, and immersion 10-20 hours sterilize, and are then rinsed well with spring or pure water.
In order to prevent and treat butt rot, 5-8 centimetres high of dendrobium candidum is removed from blake bottle before transplanting, with 50% carbendazim
800 times of dilution is soaked 10 minutes, you can is transplanted on implant bed.
Implant bed is that plantation dendrobium officinale substrate is placed on the plastic plate of perforate, and stromal thickness is not less than 8 centimetres；Plastics
It is clean cobblestone or lime stone below plate, 2-5 centimetres of particle diameter, piecemeal plantation, pavement is left in centre.
Establish net canopy above implant bed, summer takes sun-proof, and there is warming facility in winter.
Described fertilizing management；Growth of Dendrobium candidum needs to apply fertilizer, and the fertilizer that the present invention applies as needed is divided into solid fertilizer
And Liquid Fertilizer, the weight content of the raw material of solid fertilizer are：Fermented cow dung 20-30 parts；Pine needle meal 10-15 parts；Brown sugar 3-5 parts；Bone
Powder 5-10；Potassium dihydrogen phosphate 10-20；Ferrous sulfate 1-2 parts；Particle will be made in solid fertilizer, to lead to 105-110 DEG C before particle is made
Vapor carries out disinfection, and is then packed with plastic bag sealing, if it were possible, one layer of water-soluble resin of particle outer wrapping make it is slow
Release fertilizer.
The matrix and fertilizer of the present invention all contains pine tree material：Pine bark, pine tree sawdust and pine needle meal, they contain pine
Fat, some pest and disease damages can be prevented, also with soft, ventilative characteristic.
Described Liquid Fertilizer is peanut press pulp zymotic fluid, and it is that 1000 kilograms of spring add 1 kilogram of peanut press pulp, adds brown sugar
100 grams, it is put into closed container and ferments more than 5 months.
Solid fertilizer is respectively applied once in summer in spring and autumn, and Liquid Fertilizer applied fertilizer once per 20-30 days.
Application method, solid fertilizer is put into around the matrix of plantation dendrobium candidum plant, not press close to plant dendrobium candidum
Plant, allow it slowly to be dissolved under natural environment, give dendrobium candidum to absorb, typically according to the growing state of dendrobium candidum,
Every square metre of dendrobium candidum planting tray adds 0.05-0.1 kilograms.Liquid Fertilizer fertilising can use drip irrigation method.
The preventing and treating of described pest and disease damage：（l）Stem of noble dendrobium black spot blade of causing harm makes blade wither, and the 3-5 months occur.Preventing and treating side
Method：It can be sprayed l-2 times with 50% l000 times of liquid of carbendazim.（2）Stem of noble dendrobium anthracnose causes harm blade and stem branch, and aggrieved blade goes out
Existing brown or black scab, 1-5 is monthly generation.Prevention and controls：With l000 times of liquid of 50% carbendazim or 50% thiophanate methyl
1000 times of liquid are sprayed 2-3 times.（3）Stem of noble dendrobium armored scale parasitizes plant leaf edge or the back side, sucks juice, and late May is hatching
Peak period.Prevention and controls：It is as follows with the insecticide spraying that oneself is prepared, the raw material and parts by weight of insecticide：Garlic 20~30
Part, 20~30 parts of polygonum flaccidum, 1~2 part of fruit of washing one's hands, above-mentioned material is smashed, add 5~10 times of water, heating boils half an hour, takes filter
Liquid, every liter of filtrate add 5~10ml of terpinol, are diluted with water 100-300 times, are sprayed onto damaged by vermin place.
The dendrobium candidum of the present invention grows to 2 years or more than 20 centimetres and can harvested.
Advantages of the present invention：
1st, the fruit progress tissue culture for gathering the dendrobium officinale of quality better is bred, and the kind of obtained dendrobium candidum is excellent
It is good.
2nd, the culture medium effect oneself prepared is good, and emergence is neat.
3rd, the bark and pine tree sawdust of strict sterilization are crossed in the preparation of the matrix of transplanting using loose warp, dendrobium candidum disease after transplanting
Insect pest is few.
4th, the solid fertilizer and Liquid Fertilizer prepared are adapted to Growth of Dendrobium candidum needs.
5th, the preventing and treating of pest and disease damage can effectively prevent and treat stem of noble dendrobium black spot, stem of noble dendrobium anthracnose and stem of noble dendrobium armored scale etc., ensure iron
The skin stem of noble dendrobium grows.
6th, the speed of growth is fast, and active chemical content is high.
Embodiment
Embodiment 1
The present inventor carries out breeding and planting for dendrobium candidum in Guangxi Guigang Qin Tangzhen,
Tissue culture process is as follows：
The wild immature Dendrobium officinale fruit in Guangxi Mountains is plucked, is first put into the KMnO of weight concentration 1%41 point of immersion
Clock, to take out, be cut into particle with scissors, be put into the blake bottle for being pre-loaded with culture medium, cultivate 10-15 days, it is seen that seedling grows,
It is divided into several blake bottles, is further cultured for growing to after 1 centimetre of high sub-bottle again, every bottle is more than ten seedling, grows to 5-8 centimetres of height i.e.
It can be transplanted in the matrix of greenhouse；
Described culture medium process for preparation is as follows
（1）Macroelement is prepared, following raw material is added in every liter of pure water：
Potassium nitrate 18-20；Ammonium nitrate 15-18；Potassium dihydrogen phosphate 1-2；Magnesium sulfate 3-5；Calcium chloride 6-10；Ferrous sulfate
2-4；
（2）Trace element is prepared, following raw material is added in every liter of pure water：
KI 0.05-0.10；Boric acid 0.20-0,80；Manganese sulfate 1.00-2.00；Zinc sulfate 1.00-2.00；Molybdic acid
Sodium 0.01-0,04；Copper sulphate 0.002-0.005；Cobalt chloride（6 water）0.002-0.003；
（3）Organic nutrient substance is prepared, following raw material is added in every liter of pure water；
Inositol 9-12；Lysine 1-2；Glycine 0.1-0.3；Vitamin B1 0.010.02；Vitamin B6 0.05-
0.01；
（4）Prepare auxin, auxin NAA（IBA）：Obtained after the dissolving of the sodium hydrate aqueous solution of 500mg+500ml water+2%
The 1mg/1ml aqueous solution, equivalent to 1ml solution 1mg containing hormone；
（5）Prepare basic element of cell division 6BA：1mg/1ml is obtained after the dissolving of the aqueous hydrochloric acid solution of 6BA500mg+500ml water+2%
The aqueous solution, equivalent to 1ml solution 1mg containing hormone；
Culture medium process for preparation is as follows：
1st, the banana 150g of maturation is taken, 100 grams of cooked potato, adds distilled water mashing uniformly, then constant volume to 1L；
Obtain solution A；
2nd, 15 grams of brown sugar 60g, agar are weighed, is put into 2 liters of containers, adds macroelement：100m1；Organic organic nutrient substance：
20ml；Trace element：20m1；Auxin：2ml；The basic element of cell division：2ml；Obtain solution B；
3rd, solution A is added in solution B, it is stirring while adding uniform；
4th, above-mentioned solution watery hydrochloric acid or diluted sodium hydroxide solution are adjusted into pH value to 6.1-6.5；
5th, 5-10 minutes are boiled in heating；Call in while hot in tissue culture bottle, about height is 2.5-3.0cm；
6th, cover lid to tighten, be put into pressure cooker and sterilize；
7th, after sterilizing, taking-up is placed on shelf, allows its natural cooling, during which not rocked, in case agar does not solidify；
8th, it could be inoculated with after at least 24 hours after taking the dish out of the pot, length be cut off from when preferably placing a bit, it can be seen that culture medium
Whether it is contaminated.
The preparation of the matrix of transplanting：
Pine bark and pine tree sawdust are taken, wherein pine bark and pine tree sawdust weight ratio are 1:1, smash 1-5mm sizes
Particle, 10 minutes desinsections are steamed with vapor, then carried out disinfection with potassium permanganate solution, concentration is that per kilogram water adds 0.2-0.5
Gram, immersion 10-20 hours sterilize, and are then rinsed well with spring or pure water.
In order to prevent and treat butt rot, 5-8 centimetres high of dendrobium candidum is removed from blake bottle before transplanting, with 50% carbendazim
800 times of dilution is soaked 10 minutes, you can is transplanted on implant bed.
Implant bed is that plantation dendrobium officinale substrate is placed on the plastic plate of perforate, and stromal thickness is not less than 8 centimetres；Plastics
It is clean cobblestone or lime stone below plate, 2-5 centimetres of particle diameter, piecemeal plantation, pavement is left in centre.
Establish net canopy above implant bed, summer takes sun-proof, and there is warming facility in winter.
The making of solid fertilizer and Liquid Fertilizer：
The weight content of the raw material of solid fertilizer is：Fermented cow dung 20-30 parts；Pine needle meal 10-15 parts；Brown sugar 3-5 parts；Bone meal
5-10；Potassium dihydrogen phosphate 10-20；Ferrous sulfate 1-2 parts；Particle is made, leads to 105-110 DEG C of vapor and carries out disinfection, Ran Houyong
Plastic bag sealing is packed.
Liquid Fertilizer is peanut press pulp zymotic fluid, and it is that 1000 kilograms of spring add 1 kilogram of peanut press pulp, adds 100 grams of brown sugar, puts
Fermented more than 5 months to closed container.
Solid fertilizer is respectively applied once in summer in spring and autumn, and Liquid Fertilizer applied fertilizer once per 20-30 days.
Application method, solid fertilizer is put into around the matrix of plantation dendrobium candidum plant, not press close to plant dendrobium candidum plant
Strain, allows it slowly to be dissolved under natural environment, absorbs to dendrobium candidum, typically according to the growing state of dendrobium candidum, often puts down
Square rice dendrobium candidum planting tray adds 0.05-0.1 kilograms.Liquid Fertilizer fertilising can use drip irrigation method.
The preventing and treating of pest and disease damage.
（l）Stem of noble dendrobium black spot blade of causing harm makes blade wither, and the 3-5 months occur.Prevention and controls：Can with 50% carbendazim
L000 times of liquid is sprayed l-2 times.
（2）Stem of noble dendrobium anthracnose causes harm blade and stem branch, and brown or black scab occurs in aggrieved blade, and 1-5 is monthly hair
It is raw.Prevention and controls：Sprayed 2-3 times with 1000 times of liquid of l000 times of liquid of 50% carbendazim or 50% thiophanate methyl.
（3）Stem of noble dendrobium armored scale parasitizes plant leaf edge or the back side, sucks juice, and late May is hatching peak period.Preventing and treating
Method：It is as follows with the insecticide spraying that oneself is prepared, the raw material and parts by weight of insecticide：20~30 parts of garlic, polygonum flaccidum 20
~30 parts, 1~2 part of fruit of washing one's hands, above-mentioned material is smashed, add 5~10 times of water, heating boils half an hour, takes filtrate, every liter of filtrate
5~10ml of terpinol is added, is diluted with water 100-300 times, is sprayed onto damaged by vermin place.
Tissue culture of the embodiment of the present invention and the dendrobium candidum of plantation have grown to 20 centimetres of height for 18 months, and branch is full, leaf
It is emerald green, through analysis, its dendrobium candidum element A, dendrobium candidum element B, dendrobium candidum element C chemical composition with wild close, than from
The dendrobium candidum content of Hunan company purchase is higher.
Claims (1)
1. the breeding planting method of dendrobium candidum, it is characterised in that：Including reproductive process and planting process,
Described reproductive process is to gather the fruit of dendrobium candidum, and the KMnO4 for being first put into weight concentration 1% soaks 1 minute, takes out,
Particle is cut into scissors, is put into the blake bottle for being pre-loaded with culture medium, is cultivated 10-15 days, it is seen that seedling grows, and is divided into some
Individual blake bottle, sub-bottle again is further cultured for growing to after 1 centimetre of height, every bottle is more than ten seedling, grows to 5-8 centimetres of height and is transplanted to greatly
In the matrix of canopy；
Described culture medium is potato and banana is primary raw material, adds macroelement, trace element, organic nutrient substance, life
Long element, basic element of cell division 6-BA and water are made, and its proportioning is as follows：
（1）Macroelement is prepared, following raw material is added in every liter of pure water：
Potassium nitrate 18-20g；Ammonium nitrate 15-18g；Potassium dihydrogen phosphate 1-2g；Magnesium sulfate 3-5g；Calcium chloride 6-10g；Ferrous sulfate
2-4g；
（2）Trace element is prepared, following raw material is added in every liter of pure water：
KI 0.05-0.10g；Boric acid 0.20-0.80g；Manganese sulfate 1.00-2.00g；Zinc sulfate 1.00-2.00g；Molybdic acid
Sodium 0.01-0.04g；Copper sulphate 0.002-0.005g；CoCL2 6H2O 0.002-0.003g；
（3）Organic nutrient substance is prepared, following raw material is added in every liter of pure water,
Inositol 9-12g；Lysine 1-2g；Glycine 0.1-0.3g；Vitamin B1 0.01-0.02g；Vitamin B6 0.05-
0.01g；
（4）Auxin is prepared, auxin NAA is added in every liter of pure water：The sodium hydrate aqueous solution of 500mg+500ml water+2% dissolves
The 1mg/1ml aqueous solution is obtained afterwards, equivalent to 1ml solution 1mg containing hormone；
（5）Prepare basic element of cell division 6-BA：Basic element of cell division 6-BA is added in every liter of pure water：The hydrochloric acid water of 500mg+500ml water+2%
The 1mg/1ml aqueous solution is obtained after solution dissolving, equivalent to 1ml solution 1mg containing hormone；
Described culture medium process for preparation is as follows：
（1）The banana 150g of maturation is taken, 100 grams of cooked potato, adds distilled water mashing uniformly, then constant volume to 1L；
To solution A；
（2）15 grams of brown sugar 60g, agar are weighed, is put into 2 liters of containers, adds macroelement 100m1, organic nutrient substance 20ml, micro
Element 20m1, auxin 2ml, basic element of cell division 6-BA 2ml obtain solution B；
（3）Solution A is added in solution B, it is stirring while adding uniform；
（4）Above-mentioned solution watery hydrochloric acid or diluted sodium hydroxide solution are adjusted into pH value to 6.1-6.5；
（5）5-10 minutes are boiled in heating；Pour into while hot in tissue culture bottle, be highly 2.5-3.0cm；
（6）Cover lid to tighten, be put into pressure cooker and sterilize；
（7）After sterilizing, taking-up is placed on shelf, allows its natural cooling, during which not rocked, in case agar does not solidify；
（8）Could be inoculated with after at least 24 hours after taking the dish out of the pot, standing time is grown a bit, it can be seen that culture medium whether by
Pollution；
The greenhouse matrix of the transplanting is pine bark and pine tree sawdust, and wherein pine bark and pine tree sawdust weight ratio are 1:1, smash
To the particle of 1-5mm sizes, 10 minutes desinsections are steamed with water vapour, then are carried out disinfection with potassium permanganate solution, concentration is per public
Jin water adds 0.2-0.5 grams, and immersion 10-20 hours sterilize, and are then rinsed well with spring or pure water；
Described planting process includes the preventing and treating of transplanting, fertilizing management and pest and disease damage；
（1）Transplanting, in order to prevent and treat butt rot, 5-8 centimetres high of dendrobium candidum is removed from blake bottle before transplanting, with 50% it is more
800 times of bacterium spirit dilution is soaked 10 minutes, that is, is transplanted on the implant bed of dendrobium officinale substrate；Net canopy, summer are established above implant bed
It takes sun-proof, and there is warming facility in winter；
（2）Fertilizing management, fertilizer are divided into solid fertilizer and Liquid Fertilizer；
The weight content of the raw material of described solid fertilizer is：Fermented cow dung 20-30 parts；Pine needle meal 10-15 parts；Brown sugar 3-5 parts；Bone
Powder 5-10 parts；Potassium dihydrogen phosphate 10-20 parts；Ferrous sulfate 1-2 parts；Particle will be made in solid fertilizer, to lead to 105- before particle is made
110 DEG C of vapor carry out disinfection, and are then packed with plastic bag sealing, or one layer of water-soluble resin of particle outer wrapping makes sustained release
Fertilizer；
Described Liquid Fertilizer is peanut press pulp zymotic fluid, and it is that 1000 kilograms of spring add 1 kilogram of peanut press pulp, adds 100 grams of brown sugar,
Closed container is put into ferment more than 5 months；
Described solid fertilizer is respectively applied once in summer in spring and autumn, and described Liquid Fertilizer applied fertilizer once per 20-30 days；
（3）The preventing and treating of pest and disease damage；
Stem of noble dendrobium black spot, which endangers blade, makes blade wither, and the 3-5 months occur, prevention and controls：Sprayed with 50% l000 times of liquid of carbendazim
Mist l-2 times；
Stem of noble dendrobium anthracnose endangers blade and stem branch, and brown or black scab occurs in aggrieved blade, and 1-5 is monthly generation；Preventing and treating side
Method：Sprayed 2-3 times with 1000 times of liquid of l000 times of liquid of 50% carbendazim or 50% thiophanate methyl；
Stem of noble dendrobium armored scale parasitizes plant leaf edge or the back side, sucks juice, and late May is hatching peak period；Prevention and controls：With
The insecticide spraying that oneself is prepared, the raw material and parts by weight of insecticide are as follows：20~30 parts of garlic, 20~30 parts of polygonum flaccidum,
1~2 part of fruit of washing one's hands, above-mentioned material is smashed, and adds 5~10 times of water, and heating boils half an hour, takes filtrate, every liter of filtrate adds
5~10ml of terpinol, is diluted with water 100-300 times, is sprayed onto damaged by vermin place；
Described implant bed is that plantation dendrobium officinale substrate is placed on the plastic plate of perforate, and stromal thickness is not less than 8 centimetres；Modeling
It is clean cobblestone or lime stone below flitch, 2-5 centimetres of particle diameter, piecemeal plantation, pavement is left in centre.
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| CN105218174A (en) * | 2015-10-21 | 2016-01-06 | 瑞丽市岭瑞农业开发有限公司 | For the liquefaction fertilizer making method of Herba Dendrobii root |
| CN105638410A (en) * | 2016-01-05 | 2016-06-08 | 安徽霍山县草之灵中药材有限公司 | Dendrobium mountain planting method |
| CN105724213A (en) * | 2016-02-27 | 2016-07-06 | 云南农业大学 | Formula of medium for planting dendrobium officinale |
| CN105794615A (en) * | 2016-03-31 | 2016-07-27 | 唐云龙 | Planting method for dendrobium officinale |
| CN107602216B (en) * | 2017-09-11 | 2020-12-22 | 张芬 | Plant growth regulating base |
| CN107567937A (en) * | 2017-09-20 | 2018-01-12 | 贵州苗贵客贸易发展有限公司 | A kind of grape cuttage and seedling culture method |
| CN109769622A (en) * | 2017-11-10 | 2019-05-21 | 丹阳圣丽园农业发展有限公司 | A kind of antibacterial dendrobium candidum special cultivation soil |
| CN108307985A (en) * | 2017-12-27 | 2018-07-24 | 康美药业（文山）药材种植管理有限公司 | A kind of Dendrobium Chrysotoxum Lindl culture substrate and preparation method |
| CN115443894A (en) * | 2022-09-15 | 2022-12-09 | 福建省亚热带植物研究所 | Large-scale three-dimensional cultivation method and cultivation system for dendrobium officinale tissue culture seedlings in greenhouse |
| CN116897832A (en) * | 2023-07-04 | 2023-10-20 | 云南农业大学 | One-step seedling culture method for dendrobium devonianum seeds |
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| CN102845308B (en) * | 2012-09-27 | 2014-03-26 | 浙江省中药研究所有限公司 | Method for dendrobium officinale tissue culture one-step seedling |
| CN103314858A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Seed tissue culture propagation method of dendrobium candidum |
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