CN107494232A - A kind of fast breeding method of Chinese catalpa tissue-cultured seedling - Google Patents
A kind of fast breeding method of Chinese catalpa tissue-cultured seedling Download PDFInfo
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- CN107494232A CN107494232A CN201710902815.2A CN201710902815A CN107494232A CN 107494232 A CN107494232 A CN 107494232A CN 201710902815 A CN201710902815 A CN 201710902815A CN 107494232 A CN107494232 A CN 107494232A
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- chinese catalpa
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a kind of fast breeding method of Chinese catalpa tissue-cultured seedling, builds atomizing automatic control system by winter warm type heliogreenhouse seedling growth greenhouse construction nursery pond and builds the step of Chinese catalpa tissue-culture container seedling insertion nursery pond progress seedling management pot planting hardening seedling big field is transplanted in the processing of Chinese catalpa tissue-culture container seedling.Invention solves Chinese catalpa tissue-cultured seedling and cultivates time length, cultivate the problem of cost is high, it is 30 35 days that whole seedling raising process, which is included in nursery pond the time required to culture and transition canopy hardening, than 120 150 days needed for the Chinese catalpa tissue culture seedling rooting hardening that generally uses now, there is great shortening in time.Seedling-wood breeding survival rate has great raising in survival rate more than 95% compared to currently used method.
Description
Technical field
The invention belongs to the raising technology field of forestry nursery stock plant, more particularly to a kind of fast breeding of Chinese catalpa tissue-cultured seedling
Method.
Background technology
Chinese catalpa seedling stock breeding method mainly uses traditional graft technology and tissue culture technology at present.Traditional Chinese catalpa graft technology
Cultivation Chinese catalpa seedling is time-consuming longer, generally requires the Chinese catalpa stock first cultivated required for grafting, then reuses the Chinese catalpa of improved seeds
Set scion and carry out grafting, cultivated from culture stock to grafting Chinese catalpa seedling, generally requiring the time of 2 years will could transfer
The Chinese catalpa transplantation of seedlings connected is not suitable with the fast-developing demand of Modern Forestry production to big Tanaka.And by grafting time, day
Gas, the limitation in season, Chinese catalpa grafting can only be carried out in two seasons of spring and autumn.And tissue culture technology is used to carry out Chinese catalpa fine quality
Fast breeding then needs the time of 8-9 months, and traditional Chinese catalpa tissue-cultured seedling cultivates cost height, operating technology and operating environment
It is required that higher, cost of investment is also higher.Taken root in the bottle of traditional Chinese catalpa tissue-cultured seedling, spent by 3 steps of outside sprout-cultivating-bottle and hardening
Time it is longer, it is necessary to time of 4-5 months, and planting percent is not high, generally can only achieve 70-80%, and this is invisible
In just further increase cultivation cost, and it is by last 3 work in tissue culture process to cultivate Chinese catalpa tissue-cultured seedling using this technology
Sequence is combined into 2 procedures, and will shorten to the time 30-35 days.It can not be constrained compared to engrafting and cultivating Chinese catalpa seedling by season,
Shorten and cultivate time and cost.Compared to tissue culture Chinese catalpa seedling shorten cultivate the time, improve survival rate, reduce cultivation into
This.And the Chinese catalpa seedling well developed root system cultivated, the speed of growth after transplanted seedling tree are fast.It can be realized throughout the year using this technology
Annual seamless production is bred, and can cultivate required a large amount of high-quality Chinese catalpa seedlings in the short period of time.
The content of the invention
Time length is cultivated to solve Chinese catalpa tissue-cultured seedling, cultivates the problem of cost is high, the present invention provides a kind of Chinese catalpa tissue-cultured seedling
Fast breeding method, method of the invention controls automatically by winter warm type heliogreenhouse seedling growth greenhouse construction-nursery pond construction-atomizing
System Construction processed-Chinese catalpa tissue-culture container seedling processing-refine Chinese catalpa tissue-culture container seedling insertion nursery pond progress seedling management-pot planting
The step of big field of seedling-seedling is transplanted.
What the present invention was achieved through the following technical solutions, the present invention provides a kind of the quick numerous of 1. described Chinese catalpa tissue-cultured seedling
Method is educated, is comprised the steps：
1）Seedling growth greenhouse construction：Seedling growth greenhouse presses the standard working-out of northern winter warm type heliogreenhouse, and canopy face uses long-lived thin without drop plastics
Film covers；The activity with gauze is reserved before canopy to leak informaton mouth；A row is stayed to be not less than 1m in 1.5 meters away from ground eminences of rear wall of canopy
The x1m rear window with gauze leaks informaton the distance between mouth, window as 1 meter after being used as；Ground is first beaten before nursery pond is built in canopy
Thickness is more than 7 centimeters of cement, and low for the high south in north；
2）Nursery pond construction：It is required that pond insied width is 1.2m, length is depending on the actual north and south width in canopy；The height of nursery pond
For 30 centimeters；The aisle that width is no less than 50 centimeters should be reserved between adjacent nursery pond；Should be outside nursery pond during construction
South east-west direction reserve gutter, and reserve on north the aisle of the east-west direction no less than 1 meter；The south of nursery pond
Side should reserve osculum；
3）Atomizing automatic control system construction：Atomizing pipe highly is set up for 2m place far from cement flooring in the top of nursery pond,
The atomizing pipe atomizer of direction installation down；The requirement of atomizing pipe is in place of 30 centimeters away from nursery pond inside edge vertical range, i.e.,
The top of each nursery pond is installed by 2 atomizing pipes；Atomizing system is automatically controlled using the reciprocal Control electricity of electronic circulation
The method of magnet valve；
4）Seedling treatment：Tissue culture Chinese catalpa bottle seedling needs to grow more than 30 days in bottle；During use by bottle seedling from culture medium with top
Sub-cut is got off, it is desirable to can not be with some culture mediums；Chinese catalpa tissue-cultured seedling should be cleaned in time after cutting, sterilization processing；So
The Leaf Pruning below 2 centimeters sterile-processed of Chinese catalpa tissue-cultured seedling bottom is fallen afterwards, by 2 centimeters of the bottom of Chinese catalpa tissue-cultured seedling and
When be immersed in nutrient solution；Nutrient solution is poured out after being soaked 30 minutes in nutrient solution, when waiting the nutrient solution of tissue-cultured seedling base portion slightly dry
Carry out cuttage；
5）Nursery stock cuttage：Chinese catalpa tissue-cultured seedling will first use the cuttage for beating that ditch device stroke depth on seedbed is 2 centimeters in cuttage
Ditch, then Chinese catalpa tissue-cultured seedling is uprightly put into cuttage ditch, gently clutched the expanded vermiculite of Chinese catalpa tissue-cultured seedling base portion；
6）Seedbed management：Behind Chinese catalpa tissue-cultured seedling insertion seedbed, open atomizing switch and one time is sprayed thoroughly to the Chinese catalpa tissue-cultured seedling just inserted
Water；It is transferred to nursery pattern and normal management is carried out to the Chinese catalpa tissue-cultured seedling for inserting seedbed；Observation Chinese catalpa tissue culture in time is needed on daytime
The moisture evaporation situation of seedling leaf, kept the skin wet to carry out atomizing to Chinese catalpa tissue-cultured seedling in time；
7）Atomizing time interval and the grasp of micro- spray atomizing duration：The main water smoke seen on blade and tender tip；
8）Emergence opportunity：Chinese catalpa tissue-cultured seedling is cultivated 20 days on seedling bed, and all Chinese catalpa tissue-cultured seedling have grown 2 roots,
And after the simple hardening of 2-3 days, it is possible to emerge；
9）Nutrition Soil configures：After the Chinese catalpa tissue-cultured seedling cultivated on seedbed removes nursery pond, to be planted in time in nutritive cube；
Using pollution-free when preparing Nutrition Soil, garden mould, river sand, pine soil, the perlite that herbicide was not used are prepared in proportion；Specifically
Ratio needs suitably to be adjusted according to the viscosity of garden mould, the appropriate loose water-permeable and air permeable of the soil prepared；Chinese catalpa tissue-cultured seedling is after plant
Chinese catalpa tissue-cultured seedling is watered with the nebulizing spray of gondola water faucet in time；
10）Nursery stock hardening conserves：Atomizing automatic control system is installed in the transition canopy of Chinese catalpa training tissue culture seedling, by progressively subtracting
The number of few atomizing carries out hardening, by 8-10 days, after Chinese catalpa tissue-cultured seedling grows new normal blade, with regard to no longer carrying out more
Mist；Chinese catalpa tissue-cultured seedling by hardening can be transplanted in the ground of crop field in the past.
Preferably, step 2）In, the bottom laying magnetization seedbed of nursery pond, lay 3 centimeters of the Φ's of 5-6 centimetres above
Stone is as pervious bed.
Preferably, step 2）In, the perlite of 20 centimeters of mesh of thickness 50 is laid on stone, 3-4 centimetres are laid on top
Thick expanded vermiculite powder is as seedling medium.
Preferably, step 2）In, laying heating wire in nursery pond, and adapted temperature automatic control facility；Use heating
During facility, substrate temperature is adjusted in 25-27 C.
Preferably, step 3）In, atomizer uses with weight and only four outlet atomizing sprinklers of trickling filter, each sprays
The distance between head is 90 centimeters；The distance in south or the north of that shower nozzle of North and South direction most on side away from nursery pond is 45 public
Point.
Preferably, step 3）In, 2 seedling cultivation bed uses, one magnetic valve is controlled, the parallel connection one on magnetic valve
Group manually-operated gate, or each nursery pond are controlled using one group of magnetic valve.
Preferably, step 4）In, using the bottle seedling of the more generation differentiation cultures of warp；Requirement to seedling reaches 30 centimeters for height
More than, it is more than 4 blades of band, the slightly tissue culture Chinese catalpa bottle seedling of lignifying.
Preferably, step 5）In, using the incisory aluminium backing plate side edge side of tissue culture as making ditch device.
Preferably, step 7）In, specific standards are：When Chinese catalpa tissue-cultured seedling just inserts seedbed, Chinese catalpa tissue culture is kept
There is moisture film on seedling leaf, but do not have water droplet and drip, atomizing duration is 3 seconds；Atomizing time interval is Chinese catalpa group
Still having on training seedling leaf should just be sprayed during 90% moisture film；In first 5 days that nursery pond is entered in the firm cuttage of Chinese catalpa tissue-cultured seedling,
Night also will carry out atomizing moisturizing to Chinese catalpa tissue-cultured seedling；It should just extend Chinese catalpa when part Chinese catalpa tissue-cultured seedling starts root after 6 days
The spray intervals time of tissue-cultured seedling, its principle are after moisture evaporation on Chinese catalpa tissue culture seedling leaf, and 75% is remained on blade
It should just be sprayed during moisture film, atomizing duration is still 3 seconds, gradually decreases night during this period to Chinese catalpa tissue-cultured seedling
Atomizing is sprayed water；After 13 days, when the Chinese catalpa tissue-cultured seedling overwhelming majority all goes out root, can further extends atomizing interval time, and it is former
When being then that the moisture film for seeing on Chinese catalpa tissue culture seedling leaf there remains 50 %, it should just be sprayed, atomizing duration
For 3 seconds, the atomizing moisturizing to Chinese catalpa tissue-cultured seedling can be stopped at night during this period；After 17 days, when most Chinese catalpa tissue cultures
When 2 roots occurs in the root system of seedling, appropriate hardening should be just carried out；
Preferably, step 8）In, lifting specific purpose tool is used during emergence, as far as possible damaged blade and root system, and as far as possible by root less
The expanded vermiculite and perlite for fastening band are cleaned out.
Beneficial effects of the present invention
1）The present invention solves Chinese catalpa tissue-cultured seedling and cultivates time length, cultivates the problem of cost is high, is entered using the tissue-culture container seedling of Chinese catalpa
Row fast breeding；Raw material are derived from not yet taking root for tissue culture squamous subculture（40 days）Bottle seedling, the length of seedling be culture medium it
Seedling of the height in 3-4 centimetres after upper shearing.
2）It will be taken root using this technology in the bottle of tissue culture nursery stock, outside sprout-cultivating-bottle, hardening three process synthesize two procedures, contracting
The short incubation time of tissue-cultured seedling, improves plant percent, greatly reduces tissue culture cost.
3）Take root cycle time of the tissue-cultured seedling in nursery pond is 20 day（The simple refining of 2-3 days is had been subjected to during this period
Seedling）, after there are 2 roots in most Chinese catalpa tissue-cultured seedling, it is possible to which Chinese catalpa tissue-cultured seedling is planted in nutritive cube.Nutrition pot seedling exists
Can is transplanted in big Tanaka after the transition hardening of 8-10 days in transition canopy.Whole seedling raising process includes cultivating in nursery pond
Be 30-35 days the time required to transition canopy hardening, than the 120-150 needed for the Chinese catalpa tissue culture seedling rooting hardening that generally uses now
My god, there is great shortening in time.Seedling-wood breeding survival rate compares currently used side more than 95% in survival rate
Method has great raising.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is further described with reference to specific embodiment.
Embodiment 1：
1st, seedling growth greenhouse construction
Seedling growth greenhouse uses common brick and coating steel pipe skeleton construction by the standard of northern winter warm type heliogreenhouse, and thing length is 60
Rice, north and south length are 15 meters.Wall and thing gable laying warming plate are incubated afterwards；Covered using long-lived anti-dropping plastic film in canopy face
Lid；The activity for reserving 1 meter of high band gauze before canopy is leaked informaton mouth, and 1 meter of x1 rice of a row is stayed in 1.5 meters away from ground eminences of rear wall of canopy
Rear window with gauze be used as after leak informaton mouth, to substitute the distance between the mouth, window that leaks informaton as 1 meter；Ground exists in canopy
The cement that thickness is 10 centimeters is first broken before building nursery pond, and it is low for the high south in north, and north and south drop is 10 centimeters in canopy；
2nd, nursery pond construction
The pond insied width of nursery pond is 1.2 meters, actual north and south length（Remove the aisle on north and gutter in the south）For 12.8
Rice；The height of nursery pond is 30 centimeters；The aisle that width is 55 centimeters is reserved between adjacent nursery pond；Nursery pond uses Dali
Slabstone is viscous to be built.The south of nursery pond reserves 50 millimeters of Φ osculum.
3rd, atomizing automatic control system construction
In the top of nursery pond highly atomizing pipe, the atomization of direction installation down of atomizing pipe are set up far from cement flooring for 2 meters of place
Shower nozzle；2 atomizing pipes are installed in the top of each nursery pond, and every atomizing pipe is at 30 centimeters away from nursery pond inside edge vertical range
Place, atomizer use with weight and only the distance between four outlet atomizing sprinklers, each shower nozzle of trickling filter be 90 centimeters；
That shower nozzle south away from nursery pond of the North and South direction most on side（Or the north）Distance be 45 centimeters；The bottom laying of nursery pond
Magnetize seedbed, the method automatically controlled using the reciprocal Control magnetic valve of electronic circulation of atomizing system.2 nursery ponds are
One group, one magnetic valve of every 2 seedling cultivation bed uses is controlled, one group of manually-operated gate in parallel on magnetic valve.
4th, nursery pond pervious bed
The construction of nursery pond pervious bed, the clean stone of 5-6 centimetre thickness Φ 3-4 centimetres is laid in nursery pond bottom as permeable
Layer.
5th, seedling medium
Seedling medium, the perlite of 20 centimeters of mesh of thickness 50, the thick expanded vermiculite of top laying 3-4 centimetres are laid on stone
Powder.
6th, Heating system
To guarantee the annual continual nursery of progress, heating wire, and adapted temperature automatic control facility are laid in nursery pond.Make
During with Heating system, substrate temperature is adjusted in 25 C.Heating wire laying depth is about 10 centimeters away from seedling medium surface.
7th, seedling treatment
Tissue culture Chinese catalpa seedling breaks up the bottle seedling of culture using warp more generations.Requirement to seedling reaches more than 30 centimeters for height, 4 leaves of band
It is more than piece, the slightly tissue culture Chinese catalpa bottle seedling of lignifying.Standard compliant bottle seedling is sheared off from culture medium above section, it is desirable to no
Can be with some culture mediums.Chinese catalpa tissue-cultured seedling is cleaned in time after cutting, sterilization processing.Then by sterile-processed Chinese catalpa
Leaf Pruning below 2 centimeters of tree tissue-cultured seedling bottom falls, and 2 centimeters of the bottom of Chinese catalpa tissue-cultured seedling is immersed in nutrient solution in time.
Now used nutrient solution is the nutrient solution for being not added with agar and sugar.Nutrient solution is poured out after being soaked 30 minutes in nutrient solution, etc.
Cuttage is carried out when the nutrient solution of tissue-cultured seedling base portion is slightly dry.
8th, nursery stock cuttage
Chinese catalpa tissue-cultured seedling will be drawn in cuttage first using the incisory aluminium backing plate side edge side of tissue culture as ditch device is made on seedbed
Depth is 2 centimeters of cuttage ditch, then Chinese catalpa tissue-cultured seedling is uprightly put into cuttage ditch, with thumb, forefinger and middle finger gently
The expanded vermiculite of Chinese catalpa tissue-cultured seedling base portion is clutched.
9th, seedbed management
Behind Chinese catalpa tissue-cultured seedling insertion seedbed, open in time atomizing switch the Chinese catalpa tissue-cultured seedling that has just inserted is sprayed one time it is permeable.Water spray
Principle be to see the discharge outlet in nursery pond south flowing water outward, at this moment illustrate that seedling medium has sprayed, now stop spray
Water, it is transferred to nursery pattern and normal management is carried out to the Chinese catalpa tissue-cultured seedling for inserting seedbed.Observation Chinese catalpa tissue culture in time is needed on daytime
The moisture evaporation situation of seedling leaf, kept the skin wet to carry out atomizing to Chinese catalpa tissue-cultured seedling in time.
10th, the grasp of atomizing time interval and micro- spray atomizing duration：
Atomizing time interval and the grasp of micro- spray atomizing duration, the water smoke mainly seen on blade and tender tip.Specific standards
It is：When Chinese catalpa tissue-cultured seedling just inserts seedbed, having moisture film on Chinese catalpa tissue culture seedling leaf is kept, but do not have water droplet down
Drop, atomizing duration are 3 seconds.Atomizing time interval is still to have just enter during 90% moisture film on Chinese catalpa tissue culture seedling leaf
Row spraying.In first 5 days that nursery pond is entered in the firm cuttage of Chinese catalpa tissue-cultured seedling, night also will carry out atomizing moisturizing to Chinese catalpa tissue-cultured seedling.6
Can extends the spray intervals time of Chinese catalpa tissue-cultured seedling when part Chinese catalpa tissue-cultured seedling starts root after it, and its principle is to work as Chinese catalpa
It should just be sprayed when 75% moisture film is remained after the moisture evaporation on tissue culture seedling leaf, on blade, atomizing duration
It is still 3 seconds, gradually decreases atomizing water spray of the night to Chinese catalpa tissue-cultured seedling during this period.After 13 days, when Chinese catalpa tissue-cultured seedling portion big absolutely
Can when all going out root is divided further to extend atomizing interval time, its principle is to see that the moisture film on Chinese catalpa tissue culture seedling leaf is also surplus
When lower 50 %, it should just be sprayed, atomizing duration is 3 seconds, can be stopped during this period at night to Chinese catalpa
The atomizing moisturizing of tissue-cultured seedling.After 17 days, when 2 roots occurs in the root system of most Chinese catalpa tissue-cultured seedling, it should just carry out
Appropriate hardening.
11st, emerge opportunity
After Chinese catalpa tissue-cultured seedling cultivates 20 days on seedling bed, all Chinese catalpa tissue-cultured seedling have grown 2 roots, and 2-3
After it simple hardening, it is possible to emergence.Lifting specific purpose tool is used during emergence, as far as possible damaged blade and root system, and to the greatest extent less
Amount cleans out the expanded vermiculite of band on root system and perlite.
12nd, Nutrition Soil configures
After the Chinese catalpa tissue-cultured seedling cultivated on seedbed removes nursery pond, to be planted in time in nutritive cube.For in nutritive cube
The requirement of Nutrition Soil be：It is required that to have appropriate nutritional ingredient, the appropriate loose water-permeable and air permeable of the soil prepared, but can not
It is too loose, otherwise in entrucking transport and toward, because soil ball is loose, causing naked phenomenon during plant in big field, Chinese catalpa cannot be ensured
The high-survival rate of tissue-cultured seedling and the later stage Seedling height gesture of nursery stock.So prepare Nutrition Soil when can use it is pollution-free, be not used
Garden mould, river sand, pine soil, the perlite of herbicide are prepared in proportion.Specific ratio needs suitably to be adjusted according to the viscosity of garden mould
It is whole.
13rd, main points are transplanted
8x10 nutritive cube is used during plant Chinese catalpa tissue-cultured seedling.To ensure that root system of the Chinese catalpa tissue-cultured seedling in nutritive cube relaxes during plant
Exhibition, nursery stock is upright, pays attention to root system can not be caused to damage.
14th, watered after planting
Chinese catalpa tissue-cultured seedling is watered with the nebulizing spray of gondola water faucet to Chinese catalpa tissue-cultured seedling in time after plant, it is desirable to must be poured
Thoroughly, a water can continuously be poured again when pouring impermeable one time.
15th, nursery stock hardening conserves
Because the leaf photosynthesis of Chinese catalpa tissue-cultured seedling is incomplete, so original blade slightly manages not after seedling growth greenhouse is removed
When will progressively come off, Chinese catalpa tissue-cultured seedling is caused to become polished rod, so as to need length of being germinateed again from the cane of Chinese catalpa tissue-cultured seedling
Leaf, Chinese catalpa tissue-cultured seedling is caused to remove the hardening phase lengthening after nursery pond.To avoid the generation of above-mentioned phenomenon, so in Chinese catalpa tissue culture
Atomizing automatic control system is installed in the transition canopy of seedling hardening, hardening is carried out by the number for gradually reducing atomizing, by 8-10
My god, after Chinese catalpa tissue-cultured seedling grows new normal blade, it is possible to no longer carry out atomizing.So as to reach the refining for making Chinese catalpa tissue-cultured seedling
The purpose that seedling stage greatly shortens.Chinese catalpa tissue-cultured seedling by hardening can be transplanted in the ground of crop field in the past.
Certainly, described above is also not limited to the example above, the technical characteristic of the invention without description can by or
Realized, will not be repeated here using prior art；It is not to this that above example, which is merely to illustrate technical scheme,
The limitation of invention, the present invention is described in detail with reference to preferred embodiment, one of ordinary skill in the art should
Understand, the variations, modifications, additions or substitutions that those skilled in the art are made in the essential scope of the present invention
Without departure from spirit of the invention, it should also belong to claims of the invention.
Claims (10)
- A kind of 1. fast breeding method of Chinese catalpa tissue-cultured seedling, it is characterised in that：Comprise the steps：1）Seedling growth greenhouse construction：Seedling growth greenhouse presses the standard working-out of northern winter warm type heliogreenhouse, and canopy face uses long-lived thin without drop plastics Film covers；The activity with gauze is reserved before canopy to leak informaton mouth；A row is stayed to be not less than 1m in 1.5 meters away from ground eminences of rear wall of canopy The x1m rear window with gauze leaks informaton the distance between mouth, window as 1 meter after being used as；Ground is first beaten before nursery pond is built in canopy Thickness is more than 7 centimeters of cement, and low for the high south in north；2）Nursery pond construction：It is required that pond insied width is 1.2m, length is depending on the actual north and south width in canopy；The height of nursery pond For 30 centimeters；The aisle that width is no less than 50 centimeters should be reserved between adjacent nursery pond；Should be outside nursery pond during construction South east-west direction reserve gutter, and reserve on north the aisle of the east-west direction no less than 1 meter；The south of nursery pond Side should reserve osculum；3）Atomizing automatic control system construction：Atomizing pipe highly is set up for 2m place far from cement flooring in the top of nursery pond, The atomizing pipe atomizer of direction installation down；The requirement of atomizing pipe is in place of 30 centimeters away from nursery pond inside edge vertical range, i.e., The top of each nursery pond is installed by 2 atomizing pipes；Atomizing system is automatically controlled using the reciprocal Control electricity of electronic circulation The method of magnet valve；4）Seedling treatment：Tissue culture Chinese catalpa bottle seedling needs to grow more than 30 days in bottle；During use by bottle seedling from culture medium with top Sub-cut is got off, it is desirable to can not be with some culture mediums；Chinese catalpa tissue-cultured seedling should be cleaned in time after cutting, sterilization processing；So The Leaf Pruning below 2 centimeters sterile-processed of Chinese catalpa tissue-cultured seedling bottom is fallen afterwards, by 2 centimeters of the bottom of Chinese catalpa tissue-cultured seedling and When be immersed in nutrient solution；Nutrient solution is poured out after being soaked 30 minutes in nutrient solution, when waiting the nutrient solution of tissue-cultured seedling base portion slightly dry Carry out cuttage；5）Nursery stock cuttage：Chinese catalpa tissue-cultured seedling will first use the cuttage for beating that ditch device stroke depth on seedbed is 2 centimeters in cuttage Ditch, then Chinese catalpa tissue-cultured seedling is uprightly put into cuttage ditch, gently clutched the expanded vermiculite of Chinese catalpa tissue-cultured seedling base portion；6）Seedbed management：Behind Chinese catalpa tissue-cultured seedling insertion seedbed, open atomizing switch and one time is sprayed thoroughly to the Chinese catalpa tissue-cultured seedling just inserted Water；It is transferred to nursery pattern and normal management is carried out to the Chinese catalpa tissue-cultured seedling for inserting seedbed；Observation Chinese catalpa tissue culture in time is needed on daytime The moisture evaporation situation of seedling leaf, kept the skin wet to carry out atomizing to Chinese catalpa tissue-cultured seedling in time；7）Atomizing time interval and the grasp of micro- spray atomizing duration：The main water smoke seen on blade and tender tip；8）Emergence opportunity：Chinese catalpa tissue-cultured seedling is cultivated 20 days on seedling bed, and all Chinese catalpa tissue-cultured seedling have grown 2 roots, And after the simple hardening of 2-3 days, it is possible to emerge；9）Nutrition Soil configures：After the Chinese catalpa tissue-cultured seedling cultivated on seedbed removes nursery pond, to be planted in time in nutritive cube； Using pollution-free when preparing Nutrition Soil, garden mould, river sand, pine soil, the perlite that herbicide was not used are prepared in proportion；Specifically Ratio needs suitably to be adjusted according to the viscosity of garden mould, the appropriate loose water-permeable and air permeable of the soil prepared；Chinese catalpa tissue-cultured seedling is after plant Chinese catalpa tissue-cultured seedling is watered with the nebulizing spray of gondola water faucet in time；10）Nursery stock hardening conserves：Atomizing automatic control system is installed in the transition canopy of Chinese catalpa training tissue culture seedling, by progressively subtracting The number of few atomizing carries out hardening, by 10 days, after Chinese catalpa tissue-cultured seedling grows new normal blade, with regard to no longer carrying out atomizing； Chinese catalpa tissue-cultured seedling by hardening can be transplanted in the ground of crop field in the past.
- A kind of 2. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 2）In, educate Miao Chi bottom laying magnetization seedbed, lays 3 centimeters of the Φ stone of 5-6 centimetres as pervious bed above.
- A kind of 3. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 2, it is characterised in that：Step 2）In, The perlite of 20 centimeters of mesh of thickness 50 is laid above stone, laying 3-4 centimetres thick expanded vermiculite powder in top is as seedling medium.
- A kind of 4. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 2）In, educate Laying heating wire in Miao Chi, and adapted temperature automatic control facility；During using Heating system, substrate temperature is adjusted in 25-27 C.
- A kind of 5. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 3）In, mist Change shower nozzle use with weight and only the distance between four outlet atomizing sprinklers, each shower nozzle of trickling filter be 90 centimeters；It is southern and northern It it is 45 centimeters to the distance in south or the north of that shower nozzle most on side away from nursery pond.
- A kind of 6. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 3）In, 2 Seedling cultivation bed to be controlled using a magnetic valve, one group of manually-operated gate in parallel on magnetic valve, or each nursery pond use one Group magnetic valve is controlled.
- A kind of 7. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 4）In, adopt With the bottle seedling of the more generation differentiation cultures of warp；Requirement to seedling reaches more than 30 centimeters for height, more than 4 blades of band, slightly wooden The tissue culture Chinese catalpa bottle seedling of change.
- A kind of 8. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 5）In, make With the incisory aluminium backing plate side edge side of tissue culture as making ditch device.
- A kind of 9. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 7）In, tool Body standard is：When Chinese catalpa tissue-cultured seedling just inserts seedbed, having moisture film on Chinese catalpa tissue culture seedling leaf is kept, but do not have water droplet Drip, atomizing duration is 3 seconds；Atomizing time interval is just should when still having 90% moisture film on Chinese catalpa tissue culture seedling leaf This is sprayed；In first 5 days that nursery pond is entered in the firm cuttage of Chinese catalpa tissue-cultured seedling, night also will carry out atomizing benefit to Chinese catalpa tissue-cultured seedling Water；The spray intervals time of Chinese catalpa tissue-cultured seedling should just be extended when part Chinese catalpa tissue-cultured seedling starts root after 6 days, its principle is to work as After moisture evaporation on Chinese catalpa tissue culture seedling leaf, it should just be sprayed when 75% moisture film is remained on blade, atomizing is lasting Time is still 3 seconds, gradually decreases atomizing water spray of the night to Chinese catalpa tissue-cultured seedling during this period；After 13 days, when Chinese catalpa tissue-cultured seedling is exhausted Can further extends atomizing interval time when major part all goes out root, and its principle is to see the moisture film on Chinese catalpa tissue culture seedling leaf When there remains 50 %, it should just be sprayed, atomizing duration is 3 seconds, during this period can be in night stopping pair The atomizing moisturizing of Chinese catalpa tissue-cultured seedling；After 17 days, when 2 roots occurs in the root system of most Chinese catalpa tissue-cultured seedling, just should Carry out appropriate hardening.
- A kind of 10. fast breeding method of Chinese catalpa tissue-cultured seedling according to claim 1, it is characterised in that：Step 8）In, go out Lifting specific purpose tool is used during seedling, as far as possible damaged blade and root system, and as far as possible by the expanded vermiculite and perlite of band on root system less Clean out.
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