CN105165376B - A kind of cuttage breeding method of thick leaf This-problem wood - Google Patents
A kind of cuttage breeding method of thick leaf This-problem wood Download PDFInfo
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- CN105165376B CN105165376B CN201510690087.4A CN201510690087A CN105165376B CN 105165376 B CN105165376 B CN 105165376B CN 201510690087 A CN201510690087 A CN 201510690087A CN 105165376 B CN105165376 B CN 105165376B
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- 239000002023 wood Substances 0.000 title claims abstract description 13
- 238000009395 breeding Methods 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 25
- 230000001954 sterilising effect Effects 0.000 claims abstract description 23
- 238000012545 processing Methods 0.000 claims abstract description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000005520 cutting process Methods 0.000 claims description 26
- 238000005507 spraying Methods 0.000 claims description 14
- 208000027418 Wounds and injury Diseases 0.000 claims description 12
- 239000005807 Metalaxyl Substances 0.000 claims description 9
- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- 238000013138 pruning Methods 0.000 claims description 8
- 230000006378 damage Effects 0.000 claims description 7
- 208000014674 injury Diseases 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 6
- 239000006013 carbendazim Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 230000003020 moisturizing effect Effects 0.000 claims description 4
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical class C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 3
- 239000005802 Mancozeb Substances 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 238000003763 carbonization Methods 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 235000021552 granulated sugar Nutrition 0.000 claims description 3
- 239000010903 husk Substances 0.000 claims description 3
- 238000010899 nucleation Methods 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 150000003254 radicals Chemical class 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000004563 wettable powder Substances 0.000 claims description 3
- 239000011449 brick Substances 0.000 claims description 2
- 238000009933 burial Methods 0.000 claims description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical class C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 241000545744 Hirudinea Species 0.000 claims 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 229910052748 manganese Inorganic materials 0.000 claims 1
- 239000011572 manganese Substances 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000010276 construction Methods 0.000 abstract description 2
- 239000011159 matrix material Substances 0.000 description 7
- 238000011160 research Methods 0.000 description 5
- 238000007726 management method Methods 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Natural products CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
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- 239000000758 substrate Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 241000142975 Cornaceae Species 0.000 description 1
- 241000209020 Cornus Species 0.000 description 1
- 241001477883 Cornus drummondii Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
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- 238000002834 transmittance Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of cuttage breeding method of thick leaf This-problem wood, it includes preparation, sterilization, the culture of cuttage fringe bar, the processing of cuttage fringe bar, cuttage and the press in cuttage pond, insert after the step such as management and seedling replanting.The present invention has drawn the mature experience in tree cuttage reproduction technique in China's production of forestry, and preparation in cuttage pond, the culture of nutrition fringe bar, fringe bar cut and urge root processing, cuttage and press, insert after the key technique such as management carried out innovative practice, achieve significant results.Compared with prior art, the inventive method is easy to operate, easy, and has the advantages that cost is low, it is fast to take root, survival rate is high, is had broad application prospects in production of forestry and Garden Engineering construction.
Description
Technical field
The invention belongs to xylophyta reproduction technique field, and in particular to a kind of cuttage breeding method of thick leaf This-problem wood.
Background technology
In Plant Taxonomy, thick leaf Lai wooden (Cornus drummondii) belongs to Cornaceae Lai wood category (Cornus).
Defoliation small arbor.4.6~7.6m of the height of tree, 3.1~4.6m of hat width.Summer-flowering, flower pattern is peculiar, has four large-scale petals, kind
Sub- white, 8~October are ripe.Originate in US West Plain and northeast river valley area.It is this kind of form grace, with luxuriant foliage and spreading branches in leafy profusion, both
Considerable flower, fruit can be appreciated again, be excellent garden landscape and water and soil conservation seeds.In October, 2010, Jiangsu Province's forestry scientific research
Institute introduces this kind of vegetable seeds 5kg from east coast of the United States, has carried out seed propagation experiment, by taking pains to foster, successfully obtains children
Nearly 50,000 plants of seedling, and system research has been carried out to its biological characteristics and ecological habit, show that thick leaf Lai wood posture is graceful, raw
Long rapid, ornamental value height, and water-fast, waterlogging, cold-resistant, strong adaptability, are suitably answered in China the Yellow River, Yangtze river basin large area
With popularization.But this category seed have one it is prominent the characteristics of be exactly kind real outer by grease, endotesta is hard, and seed has deep dormancy,
Seed is without processing, and germination percentage is almost nil then, and this brings no small difficulty to seed propagation.Along with kind of a reality needs outsourcing,
Expensive, seedling cost is high, limits its application and popularization in China to a certain extent.Therefore, breeding way need to separately be looked for
Footpath meets to produce actual demand of the upper application to seedling.
The content of the invention
The technical problems to be solved by the invention are exactly to adapt to current thick leaf Lai wood in the production of China's agriculture and forestry and town and country ring
Large-scale application promotes the objective demand to seedling during border is built, there is provided a kind of easy, effective, survival rate is high vegetative propagation
Technical method.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
A kind of cuttage breeding method of thick leaf This-problem wood, it comprises the following steps：
(1) preparation in cuttage pond：At the beginning of 3 months, selection physical features is flat, draining is good, leeward location structure cutting bed on the sunny side.
It is in advance that place progress is appropriate smooth, debris is removed, manually builds and builds cuttage pond, and spraying facility is installed.Bottom of pond lays drainage ovum
Rock layers, gauze is laid on drainage boulder bed, rough sand is as bottom in being laid on gauze；Suppression is after laying cuttage base on bottom
Matter, cutting medium aspect is set to be slightly below the ㎝ of deck edge 3~5.
(2) sterilization：After the cuttage pond exposure that is laid in step (1) 5~7 days, with the metalaxyl aqueous solution and Evil
The mould MnZn aqueous solution carries out rotation to cutting medium and sterilized 1~2 time.
(3) culture of cuttage fringe bar：2~3 years raw treelets of robust growth are chosen as elite stand, rugosity is chosen on elite stand
0.8~1.5cm healthy and strong branch, which is used as, to be treated beta pruning bar and carries out ring injury reason, and the nutritional ingredient of beta pruning bar is treated with accumulation..
(4) processing of cuttage fringe bar：Treat that beta pruning bar is cut from wound by being managed 20~25 days through ring injury in step (3)
After obtain cuttage fringe bar, fringe bar is sequenced by the consistent order of polarity and waits to cut.When cutting, it should accomplish that otch is smooth, do not tear,
Do not tilt, 8~10cm of spike length.After the completion of cutting, by fringe bar, 50 are bundled into handle, bundled fringe bar bottom (morphology in order together
Lower end) 6~8h is rinsed in flowing water, 4~6h is soaked in liquid glucose, finally the quickly dipped 5s of root agent is urged in mixing, obtains completion and urge
The fringe bar of root processing.
(5) cuttage and press：Seeding row spacing according to the ㎝ of 3 ㎝ * 4 punches on cutting medium, using straight cutting method by step (4)
In the obtained completion fringe bar of urging root to handle insert in the hole, fringe bar axillary bud upward, exposed 2~3 ㎝ in top；To skewer after the completion of cuttage
Insert matrix and fringe bar to be suppressed, to ensure that cuttings is touched with matrix, then open spraying facility and spray 1 time to seedbed, finally
Sterilized 1 time with the Bravo aqueous solution.
(6) managed after inserting：Moisturizing and regular sterilization processing are timed to the cuttage pond after step (5) processing；Wherein, it is fixed
When moisturizing processing for daytime every 20min spray 10s, night every 40min spray 10s, keep pond in temperature be 25~35 DEG C,
Humidity is more than 70%；Regular sterilization processing is to carry out rotation sterilizing with the carbendazim aqueous solution and the Bravo aqueous solution every 7 days.Even
Continuous high temperature (more than 35 DEG C of thermal extremes) more than 3 days, with individual layer sunshade net (light transmittance 50%~80%) appropriate sunshade.
(7) seedling replanting：Treat that fringe bar bottom radical is more than 6, when root long is 6~8cm, you can carry out field-transplanting.
In step (1), described cuttage pond is built into common brick, is shaped as rectangle, and 2~3m is grown in pond, and pond is wide by 100~120
㎝, deep 40~50 ㎝ in pond, east-west, Bei Gaonan is low, the ㎝ of the discrepancy in elevation 10~15.Discharge outlet is reserved in the wall bottom of thing two, to exclude
Unnecessary infiltration moisture.
In step (1), drainage boulder bed thickness is 15~20 ㎝, in thick burial depth be 8~10 ㎝, cutting medium surface
Less than cuttage pond 3~5cm of deck edge；Wherein, described cutting medium by perlite, carbonization rice husk and vermiculite with 2：1：1 weight
Than mixing.
In step (1), described spraying facility is the automatic intermittent spraying device of Controlled by micro computer.The device by shower nozzle,
Delivery hose, valve, water receiving pipe, support and microelectronics composition.Shower nozzle is arranged on leaching and filled on support, and delivery hose one end is connected to
Shower nozzle, the other end is connected to water receiving pipe, and passes through Valve controlling.Microelectronics after specific setting by that can automatically control opening for water source
Close.
In step (2), the described metalaxyl aqueous solution is diluted with water 800 by 25wt% metalaxyl wettable powders by quality
~1000 times form, and the mould MnZn aqueous solution of Suo Shu Evil adds water 400~600 by the mould mancozeb wettable powders of 64w% Evil by quality
Form again；Wherein, the method for described rotation sterilizing is the mould MnZn water of Zai Yong Evil after first being sterilized 1 time, 3 days with the metalaxyl aqueous solution
Solution sterilization 1 time, dosage is 300~500mlm twice-2, it is desirable to even spraying, do not stay dead angle.
In step (3), the method for described ring injury reason is that branch skin is broken into wound with secateurs, and is twisted branch skin with thin wire
Tightly, using broken and bast as degree, prevent shoot vegetative from flowing back, then tied up with the wide plastic skins of 2~2.5 ㎝, prevent germ from invading
Dye.
In step (4), the spike length of the fringe bar after cutting is 8~10cm.
In step (4), the preparation method of described liquid glucose is：20~30mg white granulated sugars or sucrose are dissolved in after 1kg water i.e.
；Described mixing urges the preparation method of root agent to be：200~400mg indole-3-acetic acids and 50mg α-naphthylacetic acids are dissolved in 30
In~40mL 95v/v% ethanol water, 5~10mg boraxs and 5~10mg vitamin Bs are added1, be dissolved in water to
1kg, stir and produce.
In step (5), the Bravo aqueous solution is stirred by 15~20g 75wt% Bravos wettable powders in 10kg water
Form；Wherein, the sterilize dosage of 1 time of the Bravo aqueous solution is 300~500mlm-2。
In step (6), the described carbendazim aqueous solution is by 20~30g 50wt% carbendazol wettable powders in 10kg water
Middle stirring is formed, and the Bravo aqueous solution is stirred in 10kg water by 15~20g 75wt% Bravos wettable powders and formed；Its
In, the method for described rotation sterilizing is again with Bravo aqueous solution sterilizing 1 after first being sterilized 1 time, 3 days with the carbendazim aqueous solution
Secondary, the dosage to sterilize twice is 300~500mlm-2, it is desirable to even spraying, do not stay dead angle.
Beneficial effect：The present invention proposes a kind of thick leaf Lai wood Technique on Cuttage Propagation methods, has drawn China's production of forestry
Mature experience in middle tree cuttage reproduction technique, and preparation in cuttage pond, the culture of nutrition fringe bar, fringe bar being cut and urging
Root processing, cuttage and press, insert after the key technique such as management carried out innovative practice, achieve significant results.With showing
There is technology to compare, the inventive method is easy to operate, easy, and has the advantages that cost is low, it is fast to take root, survival rate is high, in forestry
Have broad application prospects in production and in Garden Engineering construction.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims
Invention.
Embodiment 1：
The cuttage for having carried out thick leaf Lai wood in April, 2014~2014 year June in Jiangsu Provincial Forest Science Research Inst. nursery is numerous
Experiment is grown, its process is as follows：
(1) preparation in cuttage pond：Smooth, clear auxiliary work is carried out to nursery place at the beginning of 3 months, manually builds and has built cuttage pond, and
It is mounted with automatic intermittent spraying device.Drainage cobble thick one layer of 20 ㎝ of cuttage bottom of pond tiling, the gauze of boulder bed upper berth one, gauze
Middle the rough sand thick ㎝ of upper berth 10.
(2) matrix prepares and sterilized：With perlite, carbonization rice husk and vermiculite according to weight ratio 2：1：1 ratio allotment skewer
Matrix is inserted, it is apart from the ㎝ of deck edge 5 that matrix, which stacks thickness,.Exposure 7 days after the completion of matrix, have carried out sterilization processing, that is, have used afterwards
The metalaxyl aqueous solution is He the mould MnZn aqueous solution of Evil carries out rotation sterilizing 1~2 time to matrix, i.e., first with metalaxyl aqueous solution sterilizing 1
Secondary, the mould MnZn aqueous solution of Zai Yong Evil sterilizes 1 time after 3 days, dosage 400mlm-2, it is desirable to even spraying, do not stay dead angle.First frost
Lingshui Spring solution compound method is：900 times are diluted with water with 25wt% metalaxyls wettable powder by quality to form；The mould MnZn water of Evil
Solution compound method is：500 times of water is added to form by quality with the mould mancozeb wettable powders of 64w% Evil.
(3) culture of cuttage nutrition fringe bar：25 days in advance 2~3 years raw treelets for choosing robust growth as cuttage elite stand,
1.0~1.5cm of selection rugosity healthy and strong branch, which is used as, treats beta pruning bar.Ring injury is carried out with vice and thin wire to manage, i.e., with thin
Iron wire treats that the branch skin of beta pruning bar is tightened after hindering skin, using broken and bast as degree, prevents shoot vegetative from flowing back.Wrapped up after complete
Processing.
(4) fringe bar is cut and urges root to handle：Treat that beta pruning bar is cut from wound after ring is hindered, by consistent suitable of polarity
Sequence sequences, and cuts 8~10cm of spike length degree.Branch section 50 after interception is bundled into handle in order together, bundled branch pars infrasegmentalis is immersed
Rinsed 8 hours in flowing water, after be transferred in liquid glucose and soak 6 hours, finally urge the quickly dipped 5s of root agent with mixing.The preparation method of liquid glucose
For：Sucrose or white granulated sugar 30mg, add after running water 1kg uniform stirring.Mixing urges root agent to be prepared as follows obtaining：Yin
Diindyl -3- acetic acid 400mg and α-naphthylacetic acid 50mg are dissolved in 40ml 95v/v% ethanol water, add borax 10mg,
Vitamin B110mg, it is dissolved in water to 1kg, is made after stirring.
(6) cuttage and press：First punched according to the ㎝ of 3 ㎝ × 4 seeding row spacing on slotting machine, after by the fringe bar after processing successively
It is inserted on the slotting machine after sterilizing in advance.Using straight cutting method, and make fringe bar axillary bud upward, exposed 2~3 ㎝ in top.After the completion of to base
Matter and cuttings have carried out appropriate suppression.And spray is carried out to seedbed 1 time, and thoroughly sterilized 1 time with the Bravo aqueous solution.Wherein,
The dosage that the sterilizing of the Bravo aqueous solution is 1 time is 400mlm-2。
(7) managed after inserting：Spraying and moisturizing is carried out to seedbed by automatic intermittent spraying device after inserting, sprayed per 20min daytime
Mist 10s, night spraying 10s per 40min, keep in pond that temperature change is at 25~35 DEG C, humidity more than 70%.And become according to temperature
Change has carried out appropriate sunshade with individual layer sunshade net.Every 7 days with 600 times of the 50wt% carbendazim aqueous solution and 800 times
The rotation of the 75wt% Bravo aqueous solution sterilizes once.
(8) seedling replanting：Treat that cuttings bottom is taken root, root long 6cm, radical more than 6 starts to transplant crop field.
(9) actual effect：The results showed cutting rot it is less, insert after start within 15 days generate callus, 35 days start
Take root successively, 40 days rooting rates are up to 86.34%, the average ㎝ of root long 8.21, mean elements 7.8.By seedling replanting crop field at the beginning of 6 months
Culture, transplanting survival rate 97.8%.
Embodiment 2：
The cuttage of thick leaf Lai wood is synchronously carried out in April, 2014~June in the same place of Jiangsu Provincial Forest Science Research Inst.
Reproduction test.Sterilization and substrate allocation have been carried out to slotting machine according to step (1)~(2) in this example 1.Choose life in 2 years
For healthy and strong branch on treelet as cuttings, fringe bar intercepted length is 8~10cm.Fringe bar urge at root according to step (4)
Reason, cuttage and insert after management according to step (7)~(8).As a result show, it is 65% that rooting rate is checked after 40 days, average root long
6.82 ㎝, mean elements 7.4, the transplanting survival rate 85.2% of rooted plantlet.
Embodiment 3：
The cuttage of thick leaf Lai wood is synchronously carried out in April, 2014~June in the same place of Jiangsu Provincial Forest Science Research Inst.
Reproduction test.Sterilization and substrate allocation have been carried out to slotting machine according to step (1)~(2) in this example 1.Choose life in 2 years
For healthy and strong branch on treelet as cuttings, fringe bar intercepted length is 8~10cm.Direct skewer after cuttings is soaked 3 hours with running water
Be inserted on seedbed, cuttage and insert after management according to step (7)~(8) carry out Routine Management.As a result showing, cuttings is most to rot,
Rooting rate is checked after 40 days less than 15%, averagely the ㎝ of root long 3.8, mean elements 4, the transplanting survival rate of rooted plantlet are less than
50%.
Claims (6)
1. a kind of cuttage breeding method of thick leaf This-problem wood, it is characterised in that it comprises the following steps：
(1) preparation in cuttage pond：Build cuttage pond by laying bricks or stones and sprayer unit is installed, bottom of pond laying drainage boulder bed, on drainage boulder bed
Gauze is laid, rough sand is as bottom in being laid on gauze；Cutting medium is laid on bottom；
(2) sterilization：After the cuttage pond exposure that is laid in step (1) 5~7 days, with the metalaxyl aqueous solution and the mould manganese of Evil
The zinc aqueous solution carries out rotation to cutting medium and sterilized 1~2 time；
(3) culture of cuttage fringe bar：2~3 years raw treelets of robust growth are chosen as elite stand, rugosity 0.8 is chosen on elite stand
~1.5cm healthy and strong branch conduct treats beta pruning bar and carries out ring injury reason；
(4) processing of cuttage fringe bar：By in step (3) through ring injury manage 20~25 days after after beta pruning bar is cut from wound
Obtain cuttage fringe bar, 6~8h rinsed into fringe bar bottom in flowing water after being cut to fringe bar, in liquid glucose soak 4~
6h, the quickly dipped 5s of root agent finally is urged in mixing, urge root processing to complete；
(5) cuttage and press：Seeding row spacing according to the ㎝ of 3 ㎝ * 4 punches on cutting medium, will be obtained using straight cutting method in step (4)
To completion urge root handle fringe bar insert in the hole, fringe bar axillary bud upward, exposed 2~3 ㎝ in top；To cuttage base after the completion of cuttage
Matter and fringe bar are suppressed, and are then opened spraying facility and seedbed are sprayed 1 time, are finally sterilized 1 time with the Bravo aqueous solution；
(6) managed after inserting：Moisturizing and regular sterilization processing are timed to the cuttage pond after step (5) processing；Wherein, timing is protected
Wet process is 25~35 DEG C every temperature in 40min spraying 10s, holding pond every 20min spraying 10s, night for daytime, humidity
More than 70%；Regular sterilization processing is to carry out rotation sterilizing with the carbendazim aqueous solution and the Bravo aqueous solution every 7 days；
(7) seedling replanting：Treat that fringe bar bottom radical is more than 6, when root long is 6~8cm, carry out field-transplanting；
In step (1), deep 40~50 ㎝ in cuttage pond, drainage boulder bed thickness be 15~20 ㎝, in thick burial depth be 8~10
㎝, cutting medium surface are less than cuttage pond 3~5cm of deck edge；Wherein, described cutting medium is by perlite, carbonization rice husk and leech
Stone is with 2：1：1 weight ratio mixes；
In step (2), the described metalaxyl aqueous solution by 25wt% metalaxyls wettable powder is diluted with water 800 by quality~
1000 times form, and the mould MnZn aqueous solution of Suo Shu Evil adds 400~600 times of water by the mould mancozeb wettable powders of 64w% Evil by quality
Form；Wherein, the method for described rotation sterilizing is that the mould MnZns of Zai Yong Evil are water-soluble after first being sterilized 1 time, 3 days with the metalaxyl aqueous solution
Liquid sterilizes 1 time, and the dosage to sterilize twice is 300~500mlm-2。
2. cuttage breeding method according to claim 1, it is characterised in that in step (3), the side of described ring injury reason
Method is that branch skin is broken into wound with secateurs, and is tightened branch skin with thin wire, using broken and bast as degree, prevents shoot vegetative backflow,
Then tied up with the wide plastic skins of 2~2.5 ㎝, prevent infection process.
3. cuttage breeding method according to claim 1, it is characterised in that in step (4), the spike length of the fringe bar after cutting
For 8~10cm.
4. cuttage breeding method according to claim 1, it is characterised in that in step (4), the preparation side of described liquid glucose
Method is：Produced after 20~30mg white granulated sugars or sucrose are dissolved in into 1kg water；Described mixing urges the preparation method of root agent to be：By 200
~400mg indole-3-acetic acids and 50mg α-naphthylacetic acids are dissolved in 30~40mL 95v/v% ethanol water, add 5
~10mg boraxs and 5~10mg vitamin Bs1, it is dissolved in water to 1kg, stirs and produce.
5. cuttage breeding method according to claim 1, it is characterised in that in step (5), the Bravo aqueous solution by 15~
20g 75wt% Bravos wettable powders are stirred in 10kg water and formed；Wherein, the sterilize dosage of 1 time of the Bravo aqueous solution is
300~500mlm-2。
6. cuttage breeding method according to claim 1, it is characterised in that in step (6), the described carbendazim aqueous solution
Stirred and formed in 10kg water by 20~30g 50wt% carbendazol wettable powders, the Bravo aqueous solution is by 15~20g
75wt% Bravos wettable powder is stirred in 10kg water and formed；Wherein, the method for described rotation sterilizing is first with more bacterium
Sterilized 1 time with the Bravo aqueous solution again after Lingshui Spring solution sterilization 1 time, 3 days, dosage is 300~500mlm twice-2。
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101341827A (en) * | 2008-08-28 | 2009-01-14 | 湖南省林业科学院 | Cuttage replication method on tree without bark |
| CN102939839A (en) * | 2012-11-22 | 2013-02-27 | 金陵科技学院 | Cutting seedling method of cornus stolonifera |
| CN104285629A (en) * | 2014-09-05 | 2015-01-21 | 金陵科技学院 | Root seedling breeding method for bearberry cornus macrophylla |
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| CN101341827A (en) * | 2008-08-28 | 2009-01-14 | 湖南省林业科学院 | Cuttage replication method on tree without bark |
| CN102939839A (en) * | 2012-11-22 | 2013-02-27 | 金陵科技学院 | Cutting seedling method of cornus stolonifera |
| CN104285629A (en) * | 2014-09-05 | 2015-01-21 | 金陵科技学院 | Root seedling breeding method for bearberry cornus macrophylla |
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