CN110946012A - Rapid propagation method for clonal seedling - Google Patents

Rapid propagation method for clonal seedling Download PDF

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Publication number
CN110946012A
CN110946012A CN201911002352.XA CN201911002352A CN110946012A CN 110946012 A CN110946012 A CN 110946012A CN 201911002352 A CN201911002352 A CN 201911002352A CN 110946012 A CN110946012 A CN 110946012A
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China
Prior art keywords
cutting
seedling
tray
greenhouse
water
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CN201911002352.XA
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Chinese (zh)
Inventor
蔡鲁
王延平
宋姗姗
闫龙
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Shandong Shenghao Plant Technology Co ltd
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Shandong Shenghao Plant Technology Co ltd
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Priority to CN201911002352.XA priority Critical patent/CN110946012A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14Greenhouses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • A01G9/246Air-conditioning systems
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

Abstract

A rapid propagation method of a seedling clone. The breeding method comprises ten steps of first seedling shed construction, second seedling area planning, third mist spray irrigation facility, fourth matrix preparation, fifth seedling pretreatment, sixth cutting, seventh soaking rooting agent, eighth cutting, ninth cutting disinfection and tenth cutting management. The invention is used for twig cuttage and hardwood cuttage.

Description

Rapid propagation method for clonal seedling
Technical Field
The invention relates to a rapid propagation method of a seedling clone.
Background
The survival time of the common hardwood cutting seedling is long, the survival rate is not high, the rooting effect is not good, and the influence by seasons and environment is great; the common tender branch cuttage maintenance management is troublesome, the waste of the matrix is serious and is not easy to treat, the sterilization and disinfection management and control are strict, the transplanting transition process is labor-consuming and time-consuming after the seedling is formed, and the seedling raising methods of glass greenhouses, bracket seedling raising beds and other facilities are high in investment cost and troublesome in installation and disassembly.
Disclosure of Invention
The invention aims to provide a rapid clonal seedling breeding method which effectively simplifies the working procedures of the common seedling breeding method, shortens the seedling breeding time, improves the survival rate of seedlings and facilitates the maintenance management of the seedlings after cuttage, thereby controlling the seedling breeding cost to a certain extent.
The above purpose is realized by the following technical scheme:
a rapid propagation method for a seedling clone comprises the following ten steps of first seedling shed construction, second seedling area planning, third mist spray irrigation facility, fourth substrate preparation, fifth seedling pretreatment, sixth cutting of cutting slips, seventh soaking of a rooting agent, eighth cutting, ninth cutting disinfection and tenth cutting slip management.
According to the rapid propagation method of the clonal seedling, the first-step seedling raising shed construction is that a standard sunlight greenhouse or a winter-heating greenhouse is selected and constructed in the north, the slope of the greenhouse faces south, the selected site needs to have higher terrain and can not accumulate water in rainy seasons, the transportation is convenient, water sources are convenient to take, a reservoir is constructed, a generator is arranged, air ports with insect-proof nets are arranged at the front and the back of the greenhouse, the terrain in the greenhouse is arranged to be north high, south low, and concrete is poured on the ground; reserving a drainage ditch on the south side of the greenhouse, and reserving 1m of width on the north side for later-stage water and electricity distribution; the second step of planning a seedling raising area, wherein the seedling raising area is drawn by painting from west to east in a built greenhouse, the seedling raising area is in the direction of north and south, the length of the seedling raising area is 12-15 m, the width of the seedling raising area is 1.2 m, and the interval between two adjacent areas is 60cm and is used for a passageway; in the third step of the mist spray irrigation facility, two rows of PE pipes are erected 2m above each area, the interval between the two rows of PE pipes is 0.6m, the vertical projection distance of the PE pipes is 0.3m away from the edge of each area, a hole is punched below each PE pipe to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode, and the interval between adjacent spray heads of each row of PE pipes is 0.9 m; every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
The fourth step of preparing a substrate, preparing the substrate by peat and perlite according to a proportion according to the variety of the seedling trees one day before the seedling cultivation, disinfecting by adopting 80% of mancozeb and 500 times of wettable powder, spraying the substrate with moisture, and enabling the moisture to be tightly pressed by hands to form a cluster, wherein the moisture can be properly dispersed when the hands are loose; then preparing a special tray, arranging a non-woven fabric container bag on the tray, putting the prepared substrate into the non-woven fabric container bag on the tray, and putting the tray in a seedling growing area; the fifth step of nursery stock pretreatment is to collect current-year cuttings from a cutting garden, transport the cuttings to a nursery stock pretreatment workshop, clean dirt, soil and worm eggs on the cuttings, and then immerse the cuttings in 80% carbendazim and 800 times of liquid for 3-5 minutes for disinfection; the sixth step is to trim the cutting slips, cut 45-degree bevel openings of the sterilized cutting slips from the positions 0.01-0.03m away from the leaves at the bottom, trim the damaged leaves and at least reserve 3-4 complete functional leaves; and the seventh step of soaking the rooting agent, arranging the trimmed cutting slips, neatly inserting the lower ends of all the cutting slips into a flat-bottomed container made of a plastic material, pouring an ABT rooting agent solution into the container to immerse the lower ends of the cutting slips by 0.03-0.04 m, and adjusting the concentration and the soaking time of the rooting agent according to the seedling variety.
The eighth step of cuttage, namely punching holes on the plug tray by using a clean puncher or chopsticks, vertically inserting the cutting slips soaked with the rooting agent into the plug tray for 0.03-0.05m, and slightly pinching the substrate by fingers to wrap the bottom of the cutting slips after insertion so as to keep the cutting slips vertical; then, spraying the substrate thoroughly by using a mist spray irrigation facility; sterilizing after the ninth step of cutting, and after the mist control is stopped the next day after cutting, spraying 80% carbendazim and 800 times of solution on the cutting slips for sterilization; then, spraying and disinfecting the cutting every 7 days; after the cutting of the cutting slips in the tenth step, the management is carried out, wherein the ventilation and cooling are paid attention to in spring and summer at high temperature, and the temperature is ensured by adopting a shed top cotton quilt or a shed internal heating mode in autumn and winter at low temperature; the optimum temperature in the shed is 23-27 degrees; maintaining the moisture content of the substrate at to a level where the hands are tight and cohesive, and loose to a level where they can be spread apart; and (3) supplying more water properly in the previous period after the transplanting, gradually shortening the duration of water spraying and increasing the interval time of water spraying after a large number of roots emerge, hardening the seedlings in a mode of reducing mist, and then transplanting or selling the seedlings.
The method for quickly breeding the clone of the seedling comprises the following steps that a front air opening and a rear air opening with insect-proof nets are arranged in front of and behind a first-step greenhouse, the front air opening and the rear air opening are provided with the insect-proof nets, and a hand-operated film rolling device is adopted for rolling and releasing the front air opening; the rear air inlet is a pair of windows with insect-proof nets, the height of each window is 1m, the width of each window is 0.8m, and the interval between every two adjacent windows is 1.5 m; the top of the greenhouse is provided with a cotton quilt driven by a motor, and the cotton quilt covers other areas of the greenhouse except for a rear air port; the rear air port is manually covered with a quilt to be fixedly sealed when the air temperature becomes low; in the first step, the cement ground is required to be flat without water accumulation, and the whole north, south and north are favorable for water drainage.
In the third step, two ends of each row of PE pipes are respectively provided with a ball valve for pipeline maintenance and pipeline water control; the spray heads suspended by the PE pipes are four-hole spray heads, refined mist is uniformly sprayed in four directions, and each spray head is required to be additionally provided with a heavy hammer and a drip-stop device; in the third step, each set of electromagnetic valve is connected with a set of manual ball valve in parallel.
A stock clone fast breeding method, select in the fourth step to use degradable non-woven fabrics container bag, purpose-made tray includes the top dish, the top dish open and to have a set of round hole, the bottom left side of round hole connect left tray stabilizer blade, right side and connect right tray stabilizer blade, left tray stabilizer blade with right tray stabilizer blade pass through end solid fixed disk circle and connect, end solid fixed disk circle connect the screen cloth, the round hole in insert the retainer cup, the retainer cup include retainer plate and retainer plate down, last retainer plate with lower retainer plate between connect through a set of pull rod.
In the fifth step, the collection of the cutting slips is completed before the emergence of the sun in the morning; the selected cutting is the current-year twig or semi-lignified branch; paying attention to shading and supplementing water in the process of conveying the cutting slips; during cleaning, the cutting slips are taken lightly to avoid damaging branches and leaves; in the sixth step, the direction of the cut opening is consistent with the growth direction of the leaf bud, the cutting is rapidly broken by force during pruning, and the cut opening is ensured to be smooth and regular.
The seventh step is that according to the practical production process, different seedling varieties adopt ABT rooting agents with different concentrations and are soaked for different times, other finished rooting agents or self-made rooting agents are adopted, the concentration is properly reduced, the soaking time is prolonged, and the rooting effect is better; and the lower the temperature is, the longer the soaking time is according to the difference of the room temperature; and when the eighth step is combined with the sixth step for pruning, the leaves are simply thinned, and the cutting density is ensured to allow the leaves to slightly overlap but not excessively overlap.
In the tenth step, the seedlings are directly taken out together with the non-woven fabric container bags during transplanting and sold or transplanted to a field; if the transplanting can not be carried out in time, attention is paid to shading and water conservation.
Advantageous effects
1. The invention can be used for twig cuttage and also can be used for hardwood cuttage; the seedling cuttage cultivation time is shortened; most seedling varieties can be grown in 45 days (including the seedling hardening process) by tender branch cutting, and the varieties which are easy to root can be grown in 15-20 days (including the seedling hardening process); compared with the common hardwood cutting, the seedling time is greatly reduced.
2. The invention adopts the sprinkling irrigation system and the heating system to ensure the seedling rate of the seedlings, shorten the seedling time and improve the root system quality.
3. Compared with the common cutting seedling process, the management and transplanting process is simpler, and the transition process from transplanting to a nutrition pot after seedling formation is reduced.
The invention has the advantages of large root quantity of grown seedlings and high later growth speed, and can effectively reduce the cost and improve the economic benefit when applied to production.
The branches adopted by the invention are current-year tender branches or semi-lignified branches, and the branches normally trimmed off in a nursery can be used as cuttings, so that the nursery management and seedling culture are combined.
The method can also realize winter seedling culture in the north as long as enough cuttings exist, the common cutting method of the red-leaf nandina can solve the problems, and the breeding of the red-leaf nandina is taken as an example later, so that the method can solve or reduce the problems to a certain extent.
The front and rear air ports of the greenhouse are favorable for ventilation in the greenhouse, and the air ports on the roof are favorable for discharging hot air in the greenhouse, so that the high temperature in the greenhouse in summer can be effectively reduced; the winter greenhouse is matched with the heat preservation cotton quilt and the heating wire, so that the winter seedling raising can be realized without other indoor heating measures under the condition that the cutting slips can be collected.
The semi-automatic sprinkling irrigation system adopted by the invention replenishes the cuttings with water in a timing mist spraying manner, and the environmental temperature in the greenhouse can be adjusted through the frequency of mist spraying water.
The invention adopts degradable non-woven fabric container bags for seedling culture, is easy to penetrate, easy to decompose, breathable, permeable, good in soil ball wrapping property, very convenient to transplant or sell, and capable of causing air root cutting to ensure that the transplanting survival rate is high and the growth vigor is obviously better than that of plastic container seedlings.
The invention uses degradable non-woven fabric container bags to wrap the matrix to transplant the plants together, and can effectively avoid the residue and the diffusion of pathogenic bacteria.
Compared with the mode that the medium is filled firstly and then water is difficult to spray in the common non-woven fabric container bag for seedling culture, the method can mix the medium according to the required proportion, sterilize and disinfect the medium, adjust the medium to the required humidity and then fill the medium, and is convenient to operate.
The invention realizes the modularized seedling raising mode, arranges the special tray on the hardened ground, saves the construction of hardware facilities such as a seedling raising bed or a seedling raising pond and the like, is convenient to change the position of the seedling raising area, and is simple and easy to arrange.
The special tray can be used in combination with the electric heating wire, the temperature of the matrix can be adjusted to the most suitable temperature for rooting, and the temperature of the matrix is slightly higher than the air temperature, so that the cuttings can root more easily.
The bottom of the sleeve cup and the bottom of the tray are hollow, and the tray is provided with a water outlet and support legs, so that ventilation and drainage are facilitated.
The method is relatively flexible in seedling culture, can be used for hardwood cutting and twig cutting, and can adjust the formula, the moisture, the temperature and the air circulation of the substrate at any time according to different types of the cuttings. If the cutting slips are too young and tender, a temporary shading net can be additionally covered outside the greenhouse to prevent the cutting slips from wilting.
Drawings
FIG. 1 is a schematic cross-sectional view of a seedling raising shed according to the present invention.
FIG. 2 is a schematic structural diagram of a purpose-built tray of the present invention.
FIG. 3 is a schematic structural diagram of a purpose built tray of the present invention.
Fig. 4 is a schematic structural view of the retainer cup of the present invention.
Fig. 5 is a schematic structural view of the buckle of the present invention.
FIG. 6 is a schematic view showing the placement of trays in the nursery area according to the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Example 1
A rapid propagation method for a seedling clone comprises the following ten steps of first seedling shed construction, second seedling area planning, third mist spray irrigation facility, fourth substrate preparation, fifth seedling pretreatment, sixth cutting of cutting slips, seventh soaking of a rooting agent, eighth cutting, ninth cutting disinfection and tenth cutting slip management.
Example 2
The method for quickly breeding the clonal seedlings in the embodiment 1 comprises the steps of constructing a seedling raising shed in the first step, constructing a standard sunlight greenhouse or a winter-heating greenhouse in the north, enabling the slope of the greenhouse to face the south, selecting a site with high topography and no water accumulation in rainy seasons, facilitating traffic and taking water sources, constructing a reservoir, arranging a generator to prevent the sudden situation of water and power failure, arranging air openings with insect-proof nets at the front and the rear of the greenhouse, arranging the interior topography of the greenhouse to be high in the north and low in the south, facilitating water drainage, and pouring concrete on the ground; reserving a drainage ditch on the south side of the greenhouse, and reserving 1m of width on the north side for later-stage water and electricity distribution; the second step of planning a seedling raising area, wherein the seedling raising area is drawn by painting from west to east in a built greenhouse, the seedling raising area is in the direction of north and south, the length of the seedling raising area is 12-15 m, the width of the seedling raising area is 1.2 m, and the interval between two adjacent areas is 60cm and is used for a passageway; in the third step of the mist spray irrigation facility, two rows of PE pipes are erected 2m above each area, the interval between the two rows of PE pipes is 0.6m, the vertical projection distance of the PE pipes is 0.3m away from the edge of each area, a hole is punched below each PE pipe to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode (not arranged side by side), and the interval between adjacent spray heads of each row of PE pipes is 0.9 m; every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
Example 3
The fourth step of preparing a substrate, namely preparing the substrate by peat and perlite according to a certain proportion according to the types of the seedlings one day before the seedlings are cultured, disinfecting by using 80% of mancozeb and 500 times of wettable powder solution, spraying the substrate with moisture, wherein the moisture is formed by tightly pressing hands to form a cluster, and the loose hands can be dispersed preferably; then preparing a special tray, arranging a non-woven fabric container bag on the tray, putting the prepared substrate into the non-woven fabric container bag on the tray, and putting the tray in a seedling growing area; the fifth step of nursery stock pretreatment is to collect current-year cuttings from a cutting garden, transport the cuttings to a nursery stock pretreatment workshop, clean dirt such as dust, soil, worm eggs and the like on the cuttings, and then immerse the cuttings in 80% carbendazim and 800 times of liquid for 3-5 minutes for disinfection; the sixth step is to trim the cutting slips, cut 45-degree bevel openings of the sterilized cutting slips from the positions 0.01-0.03m away from the leaves at the bottom, trim the damaged leaves and at least reserve 3-4 complete functional leaves; and the seventh step of soaking the rooting agent, tidying the trimmed cutting slips in time, neatly inserting the lower ends of all the cutting slips into a flat-bottomed container made of plastic materials, pouring an ABT rooting agent solution into the container to immerse the lower ends of the cutting slips by 0.03-0.04 m, and adjusting the concentration and the soaking time of the rooting agent according to the seedling varieties.
Example 4
The eighth step of cutting, namely, punching holes on the plug tray by using a clean puncher or chopsticks, vertically inserting the cutting slips soaked with the rooting agent into the plug tray for 0.03-0.05m, and slightly pinching the substrate by fingers to wrap the bottom of the cutting slips after insertion so as to keep the cutting slips vertical; then, spraying the substrate thoroughly by using a mist spray irrigation facility; sterilizing after the ninth step of cutting, and after the mist control is stopped the next day after cutting, spraying 80% carbendazim and 800 times of solution on the cutting slips for sterilization; then, spraying and disinfecting the cutting every 7 days; after the cutting of the cutting slips in the tenth step, the management is carried out, wherein the ventilation and cooling are paid attention to in spring and summer at high temperature, and the temperature is ensured by adopting a shed top cotton quilt or a shed internal heating mode in autumn and winter at low temperature; the optimum temperature in the shed is 23-27 degrees; the mist control is to ensure that the tender tips of the cutting shoots cannot be wilted on one hand; on one hand, water cannot be supplied too much, water accumulation of the substrate cannot be caused, the humidity of the substrate is maintained at the degree that hands are tight and conglobated, and hands are loose and can be scattered; a large amount of water is properly supplied in the previous period after the transplanting, the duration of water spraying is gradually shortened and the interval time of water spraying is increased after a large number of roots emerge, hardening seedlings by reducing mist is carried out, and then the seedlings can be transplanted or sold.
Example 5
The method for quickly breeding the clone of the nursery stock in the embodiment 1 is characterized in that a front air port and a rear air port with insect-proof nets are arranged in front of and behind a greenhouse in the first step, the front air port and the rear air port are provided with the insect-proof nets, and a hand-operated film rolling device is adopted to fold and unfold the front air port; the rear air inlet is a pair of windows with insect-proof nets, the height of each window is 1m, the width of each window is 0.8m, and the interval between every two adjacent windows is 1.5 m; the top of the greenhouse is provided with a cotton quilt driven by a motor, and the cotton quilt covers other areas of the greenhouse except for a rear air port; the rear air port is manually covered with a quilt to be fixedly sealed when the air temperature becomes low; in the first step, the cement ground is required to be flat without water accumulation, and the whole north, south and north are favorable for water drainage.
Example 6
In the third step, two ends of each row of PE pipes are respectively provided with a ball valve for pipeline maintenance and pipeline water control; the spray heads suspended by the PE pipes are four-hole spray heads, refined mist is uniformly sprayed in four directions, and each spray head is required to be additionally provided with a heavy hammer and a drip-stop device; in the third step, each set of electromagnetic valve is connected with a set of manual ball valve in parallel; to prevent emergencies such as power failure and damage of the electromagnetic valve.
Example 7
The rapid clonal propagation method of the nursery stock in the embodiment 1 comprises the following steps of selecting degradable non-woven fabric container bags, namely a specially-made tray shown in fig. 2 and 3, wherein a top tray of the specially-made tray is provided with a group of round holes 2 and a group of tray water outlets 3, the bottoms of the round holes are connected with support legs 4, 5, 12 and 13, the support legs are connected through a bottom fixing tray ring 6, the bottom fixing tray ring is connected with a screen 7, a sleeve cup 8 is inserted into the round holes, the sleeve cup comprises an upper fixing ring 9 and a lower fixing ring 10, the upper fixing ring and the lower fixing ring are connected through a group of pull rods 11, the top tray is connected with a group of left support rods 12 and a group of right support rods 13, and the left support rods and the right support rods are connected with an electric heating wire clamping groove 14; the tray assembly is clamped on the edge of the sleeve cup by a buckle 15 (specification: length, width, height, 58cm, 30cm, 5 cm), 4 seedlings can be inserted, figure 4 shows the sleeve cup (specification: diameter, height, 5.6cm, 6 cm),
the description of the using method is carried out step by step:
a) putting a non-woven fabric container bag with the diameter of 5.6cm and the length of 7cm into the sleeve cup, and enabling the non-woven fabric container bag to be attached to the wall of the sleeve cup;
b) turning outwards by 1cm higher than the cup body, clamping the cup body on the edge of the sleeve cup by using two buckles, and then sequentially putting the sleeve cup into a tray;
c) sterilizing the prepared substrate, and then putting the substrate into a special tray;
d) arranging the heating wires in a seedling raising area where the gardening ground cloth is laid (arranging the heating wires as shown in the attached figure 5);
e) the special trays are arranged in a seedling growing area paved with a gardening ground fabric as shown in the attached figure 5 (when one special tray is placed, the heating wires are required to be clamped in the clamping grooves at the bottom);
f) as shown in figure 6, the bottom of the heating wire slot is wedge-shaped, and the heating wire can be lifted upwards to slide into the slot when the heating wire is placed.
Example 8
The method for quickly breeding the clonal seedling in the embodiment 1, wherein the fifth step is to collect the cutting slips before the emergence of the sun in the morning; the selected cutting is the current-year twig or semi-lignified branch; paying attention to shading and supplementing water in the process of conveying the cutting slips; during cleaning, the cutting slips are taken lightly to avoid damaging branches and leaves; in the sixth step, the direction of the cut opening is consistent with the growth direction of the leaf bud, the cutting is rapidly broken by force during pruning, and the cut opening is ensured to be smooth and regular.
Example 9
In the method for quickly breeding the clonal seedlings in the embodiment 1, in the seventh step, in the actual production process, different varieties of seedlings need to be soaked in ABT rooting agents with different concentrations for different times (other finished rooting agents or self-made rooting agents can be adopted, and the rooting agents are indolebutyric acid and naphthylacetic acid generally prepared according to different proportions), and in the eighth step, the leaves are simply thinned when the sixth step is combined for pruning, wherein the cuttage density is that the leaves are allowed to slightly overlap but cannot be excessively overlapped.
Example 10
In the tenth step, the seedlings are taken out together with the non-woven fabric container bags directly during transplanting and sold or transplanted; if the transplanting can not be carried out in time, attention is paid to shading and water conservation (precooling treatment is needed for sale).
Example 11
The method for quickly breeding the clonal seedlings in the embodiment 2 comprises the steps of constructing a seedling raising shed in the first step, constructing a standard sunlight greenhouse or a winter-heating greenhouse in the north, enabling the inclined surface of the greenhouse to face the south, selecting a site with higher terrain and no water accumulation in rainy seasons, facilitating traffic and taking water sources, constructing a reservoir, and arranging a generator to prevent the sudden situation of water and power failure, wherein air ports with insect-proof nets are arranged at the front and the back of the greenhouse, the terrain in the greenhouse is arranged to be north high and south low, facilitating drainage and pouring concrete on the ground; reserving a drainage ditch on the south side of the greenhouse, and reserving 1m of width on the north side for later-stage water and electricity distribution; the second step of planning a seedling raising area, wherein the seedling raising area is drawn by painting from west to east in a built greenhouse, the seedling raising area is in the direction of north and south, the length of the seedling raising area is 12m, the width of the seedling raising area is 1.2 m, and the interval between two adjacent areas is 60cm and is used for a passageway; in the third step of the mist spray irrigation facility, two rows of PE pipes are erected 2m above each area, the interval between the two rows of PE pipes is 0.6m, the vertical projection distance of the PE pipes is 0.3m away from the edge of each area, a hole is punched below each PE pipe to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode (not arranged side by side), and the interval between adjacent spray heads of each row of PE pipes is 0.9 m; every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
Example 12
The method for quickly breeding the clonal seedlings in the embodiment 2 comprises the steps of constructing a seedling raising shed in the first step, constructing a standard sunlight greenhouse or a winter-heating greenhouse in the north, enabling the inclined surface of the greenhouse to face the south, selecting a site with higher terrain and no water accumulation in rainy seasons, facilitating traffic and taking water sources, constructing a reservoir, and arranging a generator to prevent the sudden situation of water and power failure, wherein air ports with insect-proof nets are arranged at the front and the back of the greenhouse, the terrain in the greenhouse is arranged to be north high and south low, facilitating drainage and pouring concrete on the ground; reserving a drainage ditch on the south side of the greenhouse, and reserving 1m of width on the north side for later-stage water and electricity distribution; the second step of planning a seedling raising area, wherein the seedling raising area is drawn by painting from west to east in a built greenhouse, the seedling raising area is in the direction of north and south, the length of the seedling raising area is 15 m, the width of the seedling raising area is 1.2 m, and the interval between two adjacent areas is 60cm and is used for a passageway; in the third step of the mist spray irrigation facility, two rows of PE pipes are erected 2m above each area, the interval between the two rows of PE pipes is 0.6m, the vertical projection distance of the PE pipes is 0.3m away from the edge of each area, a hole is punched below each PE pipe to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode (not arranged side by side), and the interval between adjacent spray heads of each row of PE pipes is 0.9 m; every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
Example 13
The method for quickly breeding the clonal seedlings in the embodiment 2 comprises the steps of constructing a seedling raising shed in the first step, constructing a standard sunlight greenhouse or a winter-heating greenhouse in the north, enabling the inclined surface of the greenhouse to face the south, selecting a site with higher terrain and no water accumulation in rainy seasons, facilitating traffic and taking water sources, constructing a reservoir, and arranging a generator to prevent the sudden situation of water and power failure, wherein air ports with insect-proof nets are arranged at the front and the back of the greenhouse, the terrain in the greenhouse is arranged to be north high and south low, facilitating drainage and pouring concrete on the ground; reserving a drainage ditch on the south side of the greenhouse, and reserving 1m of width on the north side for later-stage water and electricity distribution; the second step of planning a seedling raising area, wherein the seedling raising area is drawn by painting from west to east in a built greenhouse, the seedling raising area is in the direction of north and south, the length of the seedling raising area is 13 m, the width of the seedling raising area is 1.2 m, and the interval between two adjacent areas is 60cm and is used for a passageway; in the third step of the mist spray irrigation facility, two rows of PE pipes are erected 2m above each area, the interval between the two rows of PE pipes is 0.6m, the vertical projection distance of the PE pipes is 0.3m away from the edge of each area, a hole is punched below each PE pipe to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode (not arranged side by side), and the interval between adjacent spray heads of each row of PE pipes is 0.9 m; every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
Example 14
The fourth step of preparing a substrate, namely preparing the substrate by peat and perlite according to a certain proportion according to the types of the seedlings one day before the seedlings are cultured, disinfecting by using 80% of mancozeb and 500 times of wettable powder solution, spraying the substrate with moisture, wherein the moisture is formed by tightly pressing hands to form a cluster, and the loose hands can be dispersed preferably; then preparing a special tray, arranging a non-woven fabric container bag on the tray, putting the prepared substrate into the non-woven fabric container bag on the tray, and putting the tray in a seedling growing area; the fifth step of nursery stock pretreatment is to collect current-year cuttings from a cutting garden, transport the cuttings to a nursery stock pretreatment workshop, clean dirt such as dust, soil, worm eggs and the like on the cuttings, and then immerse the cuttings in 80% carbendazim and 800 times of liquid for 3 minutes for disinfection; the sixth step is to trim the cutting slips, cut 45-degree bevel openings of the sterilized cutting slips from the positions 0.01m away from the leaves at the bottom, trim the damaged leaves and at least reserve 3-4 complete functional leaves; and the seventh step of soaking the rooting agent, tidying the trimmed cutting slips in time, neatly inserting the lower ends of all the cutting slips into a flat-bottomed container made of a plastic material, pouring an ABT rooting agent solution into the container to immerse the lower ends of the cutting slips by 0.03m, and adjusting the concentration and the soaking time of the rooting agent according to the seedling variety.
Example 15
The fourth step of preparing a substrate, namely preparing the substrate by peat and perlite according to a certain proportion according to the types of the seedlings one day before the seedlings are cultured, disinfecting by using 80% of mancozeb and 500 times of wettable powder solution, spraying the substrate with moisture, wherein the moisture is formed by tightly pressing hands to form a cluster, and the loose hands can be dispersed preferably; then preparing a special tray, arranging a non-woven fabric container bag on the tray, putting the prepared substrate into the non-woven fabric container bag on the tray, and putting the tray in a seedling growing area; the fifth step of nursery stock pretreatment is to collect current-year cuttings from a cutting garden, transport the cuttings to a nursery stock pretreatment workshop, clean dirt such as dust, soil, worm eggs and the like on the cuttings, and then immerse the cuttings in 80% carbendazim and 800 times of liquid for 5 minutes to sterilize; the sixth step is to trim the cutting slips, cut 45-degree bevel openings of the sterilized cutting slips at the position 0.03m away from the leaves from the bottom, trim the damaged leaves and at least reserve 3-4 complete functional leaves; and the seventh step of soaking the rooting agent, tidying the trimmed cutting slips in time, neatly inserting the lower ends of all the cutting slips into a flat-bottomed container made of plastic materials, pouring an ABT rooting agent solution into the container to immerse the lower ends of the cutting slips by 0.04 m, and adjusting the concentration and the soaking time of the rooting agent according to the seedling variety.
Example 16
The eighth step of cutting, namely, punching holes on the plug tray by using a clean puncher or chopsticks, vertically inserting the cutting slips soaked with the rooting agent into the plug tray for 0.03m, and slightly pinching the substrate by fingers to wrap the bottom of the cutting slips after insertion so as to keep the cutting slips vertical; then, spraying the substrate thoroughly by using a mist spray irrigation facility; sterilizing after the ninth step of cutting, and after the mist control is stopped the next day after cutting, spraying 80% carbendazim and 800 times of solution on the cutting slips for sterilization; then, spraying and disinfecting the cutting every 7 days; after the cutting of the cutting slips in the tenth step, the management is carried out, wherein the ventilation and cooling are paid attention to in spring and summer at high temperature, and the temperature is ensured by adopting a shed top cotton quilt or a shed internal heating mode in autumn and winter at low temperature; the optimum temperature in the shed is 23 degrees; the mist control is to ensure that the tender tips of the cutting shoots cannot be wilted on one hand; on one hand, water cannot be supplied too much, water accumulation of the substrate cannot be caused, the humidity of the substrate is maintained at the degree that hands are tight and conglobated, and hands are loose and can be scattered; a large amount of water is properly supplied in the previous period after the transplanting, the duration of water spraying is gradually shortened and the interval time of water spraying is increased after a large number of roots emerge, hardening seedlings by reducing mist is carried out, and then the seedlings can be transplanted or sold.
Example 17
The eighth step of cutting, namely, punching holes on the plug tray by using a clean puncher or chopsticks, vertically inserting the cutting slips soaked with the rooting agent into the plug tray for 0.05m, and slightly pinching the substrate by fingers to wrap the bottom of the cutting slips after insertion so as to keep the cutting slips vertical; then, spraying the substrate thoroughly by using a mist spray irrigation facility; sterilizing after the ninth step of cutting, and after the mist control is stopped the next day after cutting, spraying 80% carbendazim and 800 times of solution on the cutting slips for sterilization; then, spraying and disinfecting the cutting every 7 days; after the cutting of the cutting slips in the tenth step, the management is carried out, wherein the ventilation and cooling are paid attention to in spring and summer at high temperature, and the temperature is ensured by adopting a shed top cotton quilt or a shed internal heating mode in autumn and winter at low temperature; the optimum temperature in the shed is 27 degrees; the mist control is to ensure that the tender tips of the cutting shoots cannot be wilted on one hand; on one hand, water cannot be supplied too much, water accumulation of the substrate cannot be caused, the humidity of the substrate is maintained at the degree that hands are tight and conglobated, and hands are loose and can be scattered; a large amount of water is properly supplied in the previous period after the transplanting, the duration of water spraying is gradually shortened and the interval time of water spraying is increased after a large number of roots emerge, hardening seedlings by reducing mist is carried out, and then the seedlings can be transplanted or sold.
Example 18
The rapid propagation method of the seedling clone in the embodiment comprises the following steps: in the north, a standard sunlight greenhouse or a winter-heating greenhouse is selected, the inclined plane of the greenhouse faces the south, the site selection needs to pay attention to the fact that water cannot be accumulated in rainy seasons with high terrain, traffic is convenient, water sources are convenient to take, a reservoir is built, and a generator is arranged to prevent the emergency of water and power failure. Wind ports with insect-proof nets are arranged at the front and the rear of the greenhouse, the topography in the greenhouse is entirely north high and south low, drainage is facilitated, and concrete is poured on the ground. A drainage ditch is reserved on the south side of the greenhouse, and a width of 1m is reserved on the north side of the greenhouse for later-stage water and electricity distribution.
And secondly, planning a seedling raising area: and painting seedling raising areas from west to east in the constructed greenhouse, wherein the seedling raising areas run from north to south, are 12-15 m long and 1.2 m wide, and are used for the passageways at an interval of 60cm between every two adjacent areas.
The third step is mist sprinkling irrigation facilities: two rows of PE pipes are erected at the position 2m above each area, the interval between the two rows of PE pipes is 60cm, the vertical projection distance of the PE pipes is 30cm from the edge of each area, holes are punched below the PE pipes, mist spray heads of the two rows of PE pipes are arranged in a staggered mode (not arranged side by side), and the interval between adjacent spray heads of each row of PE pipes is 90 cm. Every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
The fourth step is matrix preparation: the matrix is prepared by peat and perlite according to a certain proportion according to the types of the seedlings in one day before the seedlings are raised, 500 times of liquid of 80% wettable powder with Sensen manganese zinc is adopted for disinfection, the matrix is sprayed with moisture, the moisture is by hand to form a cluster, and the cluster can be dispersed when the hand is loosened. Then preparing a special tray, arranging a non-woven fabric container bag on the tray, putting the prepared substrate into the non-woven fabric container bag on the tray, and putting the tray in a seedling growing area.
Fifth, pretreatment of the nursery stock: collecting current-year cuttings from a female parent garden, transporting the cuttings back to a nursery stock pretreatment workshop, cleaning dirt such as dust, soil, insect eggs and the like on the cuttings, and then soaking the cuttings in 80% carbendazim 800-fold liquid for 3-5 minutes for disinfection.
Sixth, cutting the cutting shoots: cutting the disinfected cutting slips into 45-degree oblique openings at the positions 2 cm away from the leaves from the bottom, and subtracting the damaged leaves to at least reserve 3-4 complete functional leaves.
The seventh step of soaking the rooting agent: and (3) timely trimming the trimmed cutting slips to ensure that the lower ends of all the cutting slips are regularly inserted into a flat-bottom container made of plastic materials, pouring an ABT rooting agent solution into the container to ensure that the lower ends of the cutting slips are immersed for 3-4 cm, and differently adjusting the concentration of the rooting agent and the immersion time according to different seedling varieties.
Eighth step, cuttage: punching holes on the plug tray by using a clean puncher or chopsticks, vertically inserting the cutting slips soaked with the rooting agent into the plug tray for about 4 cm, slightly pinching the substrate by fingers after insertion to wrap the bottom of the cutting slips, and keeping the cutting slips vertical. Then the substrate is sprayed thoroughly by a mist spray irrigation facility.
Ninth step, disinfection after insertion: and after the mist control is stopped in the evening of the next day after cuttage, spraying and disinfecting the cuttings by using 800 times of 80% carbendazim solution. And then the cutting is sterilized by spraying once every 7 days.
The tenth step of management after cutting of the cutting: the temperature is ensured by taking care of ventilation and cooling in spring and summer at high temperature and taking care of adopting a mode of heating cotton quilts on the ceiling or in a shed to ensure the temperature in autumn and winter at low temperature. The optimum temperature in the shed is about 25 ℃. The mist control is to ensure that the tender tips of the nandina cannot be wilted on one hand; on the one hand, water cannot be supplied too much, water cannot be accumulated in the substrate, the humidity of the substrate is maintained at the degree that hands are tight and conglomerated, and hands are loose and can be scattered. The water can be supplied for a certain time before the transplanting, the duration of water spraying is gradually shortened and the interval time of water spraying is increased after a large number of roots emerge, the seedling hardening is carried out by reducing the mist, and then the seedling can be transplanted or sold.
Example 19
The rapid propagation method of the seedling clone described in the above embodiment forms a clone group through single plant tissue culture; in order to maintain the excellent characters, the breeding is usually carried out by adopting a tissue culture or cuttage mode; the rooting rate of the nandina domestica with red leaves cultivated by the method is more than 90% by adopting the tender branches, and the seedling emergence time is 25-30 days (including seedling hardening); the hard branches are adopted, so the seedling emergence time is slightly longer than that of the tender branches.
⑴ A seedling raising shed is constructed by selecting a place with high terrain, no water accumulation in rainy season, convenient traffic and convenient water source taking, constructing a standard steel structure single-layer film shed by red bricks, facing south, constructing a reservoir and arranging a generator to prevent the emergency of water and power failure, wherein the wall of the shed is a common 37-wall, the side wall is a double-layer 37-wall for mounting a thermal insulation cotton quilt for winter, foam thermal insulation boards are laid outside the wall, air ports with insect-proof nets are arranged at the front and the back of the shed, the terrain in the shed is arranged to be north high and south low, water drainage is facilitated, concrete is poured on the ground, a drainage ditch is reserved at the south side of the shed, water and electricity are reserved at the north side for later period, as shown in the following figure, a front air port and a back air port-1 are greenhouse films controlled by a hand-operated curtain rolling device, and a back air port-2 is.
⑵ planning seedling growing area, which is drawn by painting from west to east in the greenhouse, wherein the seedling growing area is in the direction of north and south, the length is 12-15 m, the width is 1.2 m, and the interval between two adjacent areas is 60cm for passage.
⑶ mist spray irrigation facility, wherein two rows of PE pipes are erected 2m above each area, the two rows of PE pipes are spaced at 60cm intervals, the vertical projection distance of the PE pipes is 30cm along the edge of each area, holes are punched below the PE pipes to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode (not arranged side by side), the interval between adjacent spray heads of the PE pipes in each row is 90cm, the sprayed mist can uniformly cover the whole seedling raising pond, each two groups of seedling raising zones share one relay for semi-automatically controlling mist spray time, each group of seedling raising ponds are provided with one electromagnetic valve, and the electromagnetic valves are connected in parallel to be provided with manual ball valves for.
The three items are the construction of infrastructure.
⑷ preparing matrix, preparing matrix from peat and pearlite according to the wood species, sterilizing with 500 times of wettable powder containing Sensen-Mn-Zn, spraying water to wet the matrix, adding to make it into mass, and loosening.
This is a preparation work before raising seedlings.
⑸ ear picking, collecting the current-year-old red-leaf Nandina domestica tender branch cutting shoot before the sunrise in the morning, and cutting at a distance of 4 cm below the growth of the multiple leaves of the plant to prevent the branch from wilting, wherein the ear picking time in sunny and rainy days is above 8 points.
⑹ transportation, transporting collected branches of Nandina domestica Thunb in the female parent garden back to the nursery stock pretreatment workshop, taking care to avoid direct sunshine and physical damage to branches and leaves during transportation, soaking all the branches in water once, and placing aside for cleaning.
⑺ cleaning, cleaning dust, mud, and ovum on the branches, and then soaking the branches in 80% carbendazim 800 times solution for 3-5 min for sterilization, and avoiding physical damage to the branches and leaves during cleaning.
⑻ pruning the cutting slips by cutting the sterilized branches at 45 deg. oblique openings at the position 0.5cm away from the bud eyes from the bottom, subtracting the damaged leaves, but retaining at least 3-4 complete functional leaves, and according to the plant characteristics of Nandina domestica with red leaves, cutting one cutting slip from the selected branch, and cutting one branch into multiple cutting slips with each cutting slip kept at about 10 cm.
⑼ soaking rooting agent, namely, trimming the pruned Nandina domestica Hayata cutting slips in time, inserting the lower ends of all the cutting slips in a flat bottom container made of plastic material, pouring a rooting agent solution of 3-4 cm and 1000ppm of mABT1 into the container, and soaking for 2 hours, wherein the concentration and the soaking time of the rooting agent are adjusted differently according to different seedling varieties.
⑽ cutting, namely punching holes on the plug tray by using a clean puncher or chopsticks, vertically inserting the cutting slips soaked with the rooting agent into the plug tray for about 4 centimeters, slightly pinching the substrate by fingers after insertion to wrap the bottom of the cutting slips so as to keep the cutting slips vertical, and then spraying water on the substrate by using a mist spray irrigation facility.
⑾ sterilizing after cutting, stopping mist control in the evening of the next day after cutting, spraying and sterilizing the cutting with 800 times of 80% carbendazim solution, and then spraying and sterilizing the cutting once every 7 days.
⑿ management after cutting, wherein the temperature is ensured by keeping ventilation and cooling in spring and summer at high temperature and by keeping the temperature by heating shed cotton quilt or shed in autumn and winter at low temperature, the optimum temperature in the shed is about 25 ℃, the mist control ensures that the tender tips of the Nandina domestica with red leaves cannot be wilted, on the one hand, water cannot be supplied too much to cause water accumulation of the matrix, the humidity of the matrix is kept at to form a cluster, and the degree of scattering can be realized by loosening hands.
⒀ seedling emergence, hardening off the seedlings when the cuttage red-leaf nandina shoots out a large amount of white roots of about 1cm, the time is about 20-25 days, and the hardening off time is about 4-5 days.
Based on the existing seedling raising technology, the method balances cost, seedling rate and seedling quality to form a whole set of seedling raising process, is suitable for twig cuttage and hardwood cuttage, is not influenced by seasons, has high survival rate and causes little damage to female parent seedlings. The special seedling tray designed by the invention emphasizes on solving the series problems of heating, root control, water drainage, survival rate improvement, seedling procedure reduction, seedling facility cost reduction and the like.
Example 20
The rapid propagation method of the clonal seedlings in the embodiment 7 comprises the following steps of selecting a degradable non-woven fabric container bag, wherein a purpose-made tray comprises a tray frame, a sleeve cup and three fixing buckle assemblies, the tray frame is provided with two groups of round holes with different specifications, 4 support legs are uniformly connected below the round holes with the larger specification, the four tray support legs are connected through a bottom fixing coil ring at a position 0.5cm away from the bottom, a screen is arranged in the bottom fixing coil ring and used for draining and ventilating matrix, the sleeve cup is inserted into the round hole with the larger specification, each sleeve cup is matched with two fixing buckles, and the round hole with the smaller specification is a water outlet. Between two adjacent round holes of the round hole with larger size, the middle of the adjacent support leg is provided with an electric heating wire clamping groove for placing an electric heating wire to supply heat for the matrix.

Claims (10)

1. A rapid propagation method of a seedling clone is characterized by comprising the ten steps of first-step seedling shed construction, second-step seedling area planning, third-step mist spray irrigation facility, fourth-step matrix preparation, fifth-step seedling pretreatment, sixth-step cutting, seventh-step root-growing agent soaking, eighth-step cutting, ninth-step post-cutting disinfection and tenth-step post-cutting management of cutting.
2. The method of claim 1, wherein the first step of constructing a seedling shed comprises constructing a standard solar greenhouse or a winter-heating greenhouse in the north, wherein the greenhouse has a slope facing the south, and is located at a higher position to prevent water accumulation in rainy seasons, so that the transportation is convenient, water sources can be conveniently used, a reservoir is constructed, a generator is installed, air ports with insect-proof nets are arranged at the front and the back of the greenhouse, the soil in the greenhouse is arranged to be north high, south low, and concrete is poured on the ground; reserving a drainage ditch on the south side of the greenhouse, and reserving 1m of width on the north side for later-stage water and electricity distribution; the second step of planning a seedling raising area, wherein the seedling raising area is drawn by painting from west to east in a built greenhouse, the seedling raising area is in the direction of north and south, the length of the seedling raising area is 12-15 m, the width of the seedling raising area is 1.2 m, and the interval between two adjacent areas is 60cm and is used for a passageway; in the third step of the mist spray irrigation facility, two rows of PE pipes are erected 2m above each area, the interval between the two rows of PE pipes is 0.6m, the vertical projection distance of the PE pipes is 0.3m away from the edge of each area, a hole is punched below each PE pipe to suspend mist spray heads, the mist spray heads of the two rows of PE pipes are arranged in a staggered mode, and the interval between adjacent spray heads of each row of PE pipes is 0.9 m; every two sets of nursery sites share one set of relay and are used for semi-automatic control atomizing time, and one set of solenoid valve is installed to every group nursery pond, and the manual ball valve of parallelly connected installation of solenoid valve is spare.
3. The method for rapidly breeding the clone of the seedling as claimed in claim 1, wherein the fourth step of preparing the substrate is that the substrate is prepared by peat and perlite according to the proportion according to the variety of the seedling in the day before the seedling is grown, 80% of mancozeb and 500 times of wettable powder are adopted for disinfection, the substrate is sprayed with wet water, the humidity is that the substrate is tightly agglomerated by hands , and the loose hands can be scattered properly; then preparing a special tray, arranging a non-woven fabric container bag on the tray, putting the prepared substrate into the non-woven fabric container bag on the tray, and putting the tray in a seedling growing area; the fifth step of nursery stock pretreatment is to collect current-year cuttings from a cutting garden, transport the cuttings to a nursery stock pretreatment workshop, clean dirt, soil and worm eggs on the cuttings, and then immerse the cuttings in 80% carbendazim and 800 times of liquid for 3-5 minutes for disinfection; the sixth step is to trim the cutting slips, cut 45-degree bevel openings of the sterilized cutting slips from the positions 0.01-0.03m away from the leaves at the bottom, trim the damaged leaves and at least reserve 3-4 complete functional leaves; and the seventh step of soaking the rooting agent, arranging the trimmed cutting slips, neatly inserting the lower ends of all the cutting slips into a flat-bottomed container made of a plastic material, pouring an ABT rooting agent solution into the container to immerse the lower ends of the cutting slips by 0.03-0.04 m, and adjusting the concentration and the soaking time of the rooting agent according to the seedling variety.
4. The method for rapid propagation of clonal seedling as claimed in claim 1, wherein in the eighth step of cutting, a hole is punched on the plug tray by a clean puncher or chopsticks, the cutting shoot soaked with the rooting agent is vertically inserted into the plug tray by 0.03-0.05m, and after the insertion, the base material is slightly pinched by fingers to wrap the bottom of the cutting shoot, so that the cutting shoot is kept vertical; then, spraying the substrate thoroughly by using a mist spray irrigation facility; sterilizing after the ninth step of cutting, and after the mist control is stopped the next day after cutting, spraying 80% carbendazim and 800 times of solution on the cutting slips for sterilization; then, spraying and disinfecting the cutting every 7 days; after the cutting of the cutting slips in the tenth step, the management is carried out, wherein the ventilation and cooling are paid attention to in spring and summer at high temperature, and the temperature is ensured by adopting a shed top cotton quilt or a shed internal heating mode in autumn and winter at low temperature; the optimum temperature in the shed is 23-27 degrees; maintaining the moisture content of the substrate at to a level where the hands are tight and cohesive, and loose to a level where they can be spread apart; and (3) supplying more water properly in the previous period after the transplanting, gradually shortening the duration of water spraying and increasing the interval time of water spraying after a large number of roots emerge, hardening the seedlings in a mode of reducing mist, and then transplanting or selling the seedlings.
5. The rapid propagation method of a clonal seedling as claimed in claim 1, wherein a front air port and a rear air port with insect-proof nets are arranged in front of and behind the first-step greenhouse, the front air port and the rear air port are provided with insect-proof nets, and the front air port is retracted by a hand-operated film rolling device; the rear air inlet is a pair of windows with insect-proof nets, the height of each window is 1m, the width of each window is 0.8m, and the interval between every two adjacent windows is 1.5 m; the top of the greenhouse is provided with a cotton quilt driven by a motor, and the cotton quilt covers other areas of the greenhouse except for a rear air port; the rear air port is manually covered with a quilt to be fixedly sealed when the air temperature becomes low; in the first step, the cement ground is required to be flat without water accumulation, and the whole north, south and north are favorable for water drainage.
6. The rapid propagation method of a clonal seedling as claimed in claim 1, wherein in the third step, two ends of each row of PE pipes are respectively provided with a ball valve for pipeline maintenance and pipeline water control; the spray heads suspended by the PE pipes are four-hole spray heads, refined mist is uniformly sprayed in four directions, and each spray head is required to be additionally provided with a heavy hammer and a drip-stop device; in the third step, each set of electromagnetic valve is connected with a set of manual ball valve in parallel.
7. The method for rapid clone breeding of seedling as claimed in claim 1, wherein in the fourth step, degradable non-woven fabric bags are selected, the purpose-made tray comprises a top tray, the top tray is provided with a set of round holes, the left side of the bottom of the round holes is connected with a left tray support leg, the right side of the bottom of the round holes is connected with a right tray support leg, the left tray support leg is connected with the right tray support leg through a bottom fixing tray ring, the bottom fixing tray ring is connected with the screen, a sleeve cup is inserted into the round holes, the sleeve cup comprises an upper fixing ring and a lower fixing ring, and the upper fixing ring is connected with the lower fixing ring through a set of pull rods.
8. The method for rapid propagation of clonal seedling as claimed in claim 1, wherein the collection of cutting shoot in the fifth step is completed before sunrise in the morning; the selected cutting is the current-year twig or semi-lignified branch; paying attention to shading and supplementing water in the process of conveying the cutting slips; during cleaning, the cutting slips are taken lightly to avoid damaging branches and leaves; in the sixth step, the direction of the cut opening is consistent with the growth direction of the leaf bud, the cutting is rapidly broken by force during pruning, and the cut opening is ensured to be smooth and regular.
9. The rapid propagation method of a clonal seedling as claimed in claim 1, wherein the seventh step is to adopt ABT rooting agent with different concentrations and soak for different time according to different seedling varieties in the actual production process, adopt other finished rooting agent or self-made rooting agent to properly reduce the concentration and prolong the soaking time, and the soaking time is prolonged as the temperature is lower according to different room temperature; and the eighth step is combined with the sixth step for trimming, the leaves are simply thinned, and the cutting density is that the leaves are slightly overlapped but not excessively overlapped.
10. The method for rapid propagation of clonal seedlings as claimed in claim 1, wherein in the tenth step, the seedlings are directly taken out together with non-woven fabric container bags for sale or transplantation in the field; if the transplanting can not be carried out in time, attention is paid to shading and water conservation.
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CN107466784A (en) * 2017-09-15 2017-12-15 山东圣豪植物科技有限公司 A kind of clone tealeaves seedling fast breeding method
CN107494232A (en) * 2017-09-29 2017-12-22 山东圣豪植物科技有限公司 A kind of fast breeding method of Chinese catalpa tissue-cultured seedling
KR101920185B1 (en) * 2018-07-28 2019-02-08 경상대학교산학협력단 Method for rooted cutting propagation of strawberry

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111758405A (en) * 2020-07-30 2020-10-13 山东华玫生物科技有限公司 Full-automatic neon spraying hole tray cutting seedling method for purple roses
CN112219577A (en) * 2020-10-26 2021-01-15 四川七彩林科股份有限公司 Cutting propagation method of wire netting

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Application publication date: 20200403