CN114766217A - Vanilla cutting breeding method - Google Patents
Vanilla cutting breeding method Download PDFInfo
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- CN114766217A CN114766217A CN202210593068.XA CN202210593068A CN114766217A CN 114766217 A CN114766217 A CN 114766217A CN 202210593068 A CN202210593068 A CN 202210593068A CN 114766217 A CN114766217 A CN 114766217A
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- 238000009395 breeding Methods 0.000 title description 2
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- 244000263375 Vanilla tahitensis Species 0.000 claims abstract description 34
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- 230000001737 promoting effect Effects 0.000 claims abstract 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
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- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 7
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- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 5
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- 210000005069 ears Anatomy 0.000 abstract description 2
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a cutting propagation method of vanilla, which comprises the following steps: (1) preparing a cutting medium; (2) preparing a seedling raising plug tray; (3) seedling raising; (4) management: after 1d-2d of cuttage, spraying a bactericide, and spraying or spraying a compound fertilizer solution at the 15 th day; at 25d, opening coconut granules on stem segments of the female vines, cutting off the middle of the stem segments of the female vines to enable the female vines to form single-leaf stem segments and single-hole holes, spraying a germination promoter, and disinfecting the cut; after 30 days, spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time, and then spraying the compound fertilizer solution or spraying the foliar fertilizer at intervals of 7-10 days; (5) when the sprout grows to be more than 30cm, the sprout can be outplanted or transplanted. The invention utilizes the plug to insert the mother vines for seedling, induces each stem node of the mother vines to take root, and divides the stem node into single (leaf) nodes for accelerating germination, thereby reducing the infection diseases of the cut, reducing the occurrence of soft (stem) rot, promoting the early germination of the stem nodes, and improving the seedling emergence quantity and the seedling rate of the mother ears in unit length.
Description
Technical Field
The invention relates to the technical field of plant cultivation containers and cultivation, in particular to a cutting propagation method of vanilla.
Background
At present, the propagation mode of vanilla seedlings is mainly cutting propagation. In production, strong stem segments (mother vines) with the length of 60-80cm or 3-5 sections are mainly adopted to be directly planted in a field for production, or cuttage is carried out on a seedbed, and the strong stem segments are planted out of a nursery after being cultured into cutting seedlings. The former has the problems of large demand of mother vines, high management cost in the early stage of seedling culture, unsuitability for large-scale planting and the like, and the latter has the problems that the root system is easy to damage when cutting seedlings are lifted, the quality and later growth of the seedlings are influenced, watering is needed after lifting the seedlings, field planting needs to be completed within 1-2d, and the storage is inconvenient and long-distance transportation is not suitable.
Disclosure of Invention
The invention aims at the problems that the seedling propagation cost is high, the seedlings cannot be produced in a large scale, the root system is easy to damage when the cutting seedlings are lifted, the quality and later growth of the seedlings are affected, the cutting seedlings need to be watered to preserve moisture after being lifted, the field planting needs to be completed within 1d-2d, the storage is inconvenient, and the long-distance transportation is not suitable.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
a cutting propagation method for vanilla comprises the following operation steps:
(1) preparing a cutting medium;
(2) preparing a seedling raising plug tray;
(3) seedling culture: shearing robust vanilla mother vines (2 sections of growing points at the top end are removed) which are 5-10cm longer than the transverse length of the seedling culture plug tray, disinfecting the cuts of the robust vanilla mother vines, placing the healthy mother vines along the U-shaped holes in the concave surface of the plug tray, clamping the mother vines in the U-shaped holes, and then respectively penetrating the two ends of the mother vines through the round holes in the two sides of the plug tray to enable the cuts at the two ends of the mother vines to be exposed out of the plug tray; then slightly straightening or moving the mother vines to enable each stem node of the mother vines to be positioned in the hole; after the hole tray is full, spraying a rooting agent to axilla of the leaf of the stem node of the mother vine by using a handheld small spraying pot, after the liquid medicine is dried, scattering coconut granules to fill and level the hole tray to cover the stem node, and exposing all leaves out of the substrate surface to complete cuttage;
(4) management: after 1d-2d of cuttage, spraying a bactericide, keeping the humidity in the greenhouse at 75-85%, keeping the temperature at 20-30 ℃, and spraying or sprinkling a compound fertilizer solution at 15 d; at 25d, opening coconut grains on stem segments of the female vines, cutting off the middle of the stem segments of the female vines by using clean scissors to enable the female vines to form single (node) leaf stem segments and single hole holes, spraying a germination promoter, and disinfecting the cuts; after 30 days, spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time, and then spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time at intervals of 7-10 days;
(5) when the sprouts grow to be more than 30cm, the sprouts can be outplanted or transplanted to a field for planting, or temporarily planted in a nutrition cup for culturing seedlings; when seedlings are lifted, the two sides of the bottom of the hole are slightly pinched, and the roots of the vanilla with the matrix can be completely taken out by slightly lifting the seedlings; the seedling lifting and boxing of the vanilla or the boxing with the hole tray do not affect the long-distance transportation.
Preferably, the cutting medium in the step (1) is prepared by mixing peat, mushroom dregs, garden soil and decomposed organic fertilizer according to a volume ratio of 1: 1: 0.3: 0.5, adding water, uniformly stirring until the mass water content of the substrate reaches 60-80 percent and the pH value is 6.0-6.5, and then piling for more than 15 days to obtain the cuttage substrate.
Preferably, the decomposed organic fertilizer is obtained by fermenting an organic matter with the animal manure content of more than or equal to 45 percent (wt%) for 90 days at normal temperature; the mushroom residue is a waste stick after harvesting bag-cultivated edible fungi such as oyster mushroom, phoenix mushroom, agrocybe cylindracea and the like.
Preferably, the seedling raising tray in the step (2) is prepared by loading the cutting matrix obtained after the stack retting into the tray, and slightly pressing to ensure that the matrix is not loosened, wherein the height of the loaded matrix is lower than or equal to the concave surface of the hole.
Preferably, the seedling raising plug tray in the step (2) is provided with a U-shaped hole between the transversely adjacent plug holes of the seedling raising plug tray, and the first plug hole and the last plug hole in the transverse row, namely, two round holes facing outwards are respectively arranged on two sides of each transverse row.
Preferably, the coconut grain in the step (3) is soaked for 6-8h and has a size of 1-3 cm.
Preferably, the bactericide in the step (4) is carbendazim wettable powder solution which is obtained by diluting carbendazim wettable powder with the mass concentration of 70% by 800 times; in the 15 th day, spraying or sprinkling a compound fertilizer (15-15-15) solution A with the mass concentration of 0.2%; and after 30d, spraying the compound fertilizer solution B or spraying the foliar fertilizer for 1 time, and then spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time at intervals of 7d-10 d.
Preferably, the foliar fertilizer B is a compound fertilizer (15-15-15) solution with the mass concentration of 0.2-0.5%, and the foliar fertilizers are Huabao No. 2 and No. 5 foliar fertilizers.
Preferably, the germination promoter in step (4) is a mixed solution of 2500 times of 24-epibrassinolide solution with a mass concentration of 0.01% and 1500 times of gibberellic acid emulsifiable solution with a mass concentration of 4%.
Compared with the prior art, the invention has the following beneficial effects:
the method utilizes the plug to cut the mother vines for seedling culture, and after each stem section of the mother vines is induced to root, the stem sections cut into single (leaf) sections are divided for germination acceleration, so that the infection diseases of incisions can be reduced, the occurrence of soft (stem) rot can be reduced, the early germination of the stem sections can be promoted, and the seedling emergence quantity and the seedling rate of the mother ears in unit length can be improved; the single-plant cutting seedling with one leaf, one bud and one hole formed by cutting and segmented seedling raising through the hole tray has the advantages of soil-carrying seedling raising, complete root system and difficult damage, and is favorable for storing, packaging and transporting or temporary planting and culturing large seedlings.
Drawings
FIG. 1 is a schematic view of a seedling raising tray of the present invention; 1-seedling raising plug tray, 2-U-shaped hole and 3-round hole.
Detailed Description
The following detailed description is to be read in connection with the accompanying drawings, but it is to be understood that the scope of the invention is not limited to the specific embodiments. The raw materials and reagents used in the examples were all commercially available unless otherwise specified.
As shown in figure 1, the seedling raising plug 1 used in the following embodiment is provided with a U-shaped hole 2 between adjacent plug holes in the transverse direction of the seedling raising plug, and a round hole 3 facing outwards is arranged at the first plug hole and the last plug hole in the transverse row, namely at both sides of each transverse row. The garden soil in the embodiment is collected from the soil where flowers are planted in the jasmine garden of the floriculture institute of Guangxi agricultural academy, and is idle after disinfection and is stacked for more than 1 year.
Example 1
A cutting propagation method of vanilla comprises the following specific operation steps:
(1) preparing a cutting medium: peat, mushroom dregs (oyster mushroom), garden soil and decomposed organic fertilizer are mixed according to the volume ratio of 1: 1: 0.3: 0.5, adding water, uniformly stirring until the mass water content of the substrate reaches 60-80% and the pH value is 6.0-6.5, and then composting for 15d to obtain a cuttage substrate; the decomposed organic fertilizer is obtained by fermenting organic matters with the animal waste content of more than or equal to 45 percent (wt%) for 90 days at normal temperature;
(2) preparing a seedling raising plug: putting the cutting matrix obtained after retting into a seedling raising plug tray, and slightly pressing to ensure that the matrix is not loose, wherein the height of the put matrix is lower than or equal to the concave surface of the plug hole;
(3) seedling culture: seedling in a rain shelter, flat ground is used as a seedbed, the length and the width of the seedbed can be determined according to the size of a plug tray, the ground condition and the like, and the daily management is convenient; a layer of sunshade net with 70% is covered on the shed roof above the seedbed; shearing robust vanilla mother vines (2 sections of growing points at the top end are removed) which are 5-10cm longer than the transverse length of the seedling culture plug tray, disinfecting the cuts of the robust vanilla mother vines, placing the healthy mother vines along the U-shaped holes in the concave surface of the plug tray, clamping the mother vines in the U-shaped holes, and then respectively penetrating the two ends of the mother vines through the round holes in the two sides of the plug tray to enable the cuts at the two ends of the mother vines to be exposed out of the plug tray; then slightly straightening or moving the female vine to enable each stem node of the female vine to be positioned in the hole; after the plug tray is full, spraying a rooting agent to axilla of the leaf of the mother vine stem node by using a handheld small spraying pot, after the liquid medicine is dried, filling the plug tray with coconut grains which are soaked for 6 hours and have the size of 1-3cm so as to cover the stem node, and exposing all the leaves out of the substrate surface to complete cuttage;
(4) management: after 1-2d of cuttage, drenching a carbendazim wettable powder solution (the carbendazim wettable powder with the mass concentration of 70 percent is obtained after being diluted by 800 times), then keeping the humidity in the greenhouse at 75-85 percent, keeping the temperature at 20-30 ℃, and drenching or spraying a compound fertilizer (15-15-15) solution A with the mass concentration of 0.2 percent at the 15 th day; at the 25 th day, picking coconut grains on stem segments of the female vines, cutting off the middle of the stem segments of the female vines by using clean scissors to enable the female vines to form stem segments with single (node) leaves and single hole, spraying a germination accelerator (the germination accelerator is a mixed solution of 2500 times of 24-epibrassinolide liquid with the mass concentration of 0.01% and 1500 times of gibberellic acid missible oil liquid with the mass concentration of 4%), and disinfecting the cut; after 30 days, spraying a compound fertilizer (15-15-15) solution with the mass concentration of 0.2-0.5% or spraying a foliar fertilizer No. 2 or No. 5 for 1 time, and then spraying a compound fertilizer (15-15-15) solution with the mass concentration of 0.2-0.5% for 1 time at intervals of 7-10 days;
(5) when the sprout grows to 30cm, the sprout can be outplanted or transplanted to a field for planting, or temporarily planted in a nutrition cup for culturing a big seedling; when lifting seedlings, lightly pinching two sides of the bottom of the hole, and lightly lifting the seedlings to completely take out the root system of the vanilla with the matrix; the seedling lifting and boxing of the vanilla or the boxing with the hole tray do not affect the long-distance transportation.
Example 2
A cutting propagation method of vanilla comprises the following specific operation steps:
(1) preparing a cutting medium: peat, mushroom residue (phoenix mushroom), garden soil and decomposed organic fertilizer are mixed according to the volume ratio of 1: 1: 0.3: 0.5, adding water, uniformly stirring until the mass water content of the substrate reaches 60-80% and the pH value is 6.0-6.5, and then composting for 15d to obtain a cuttage substrate; the decomposed organic fertilizer is obtained by fermenting organic matters with the animal waste content of more than or equal to 45% for 90 days at normal temperature;
(2) preparing a seedling raising plug: putting the cutting matrix obtained after retting into a seedling raising plug tray, and slightly pressing to ensure that the matrix is not loose, wherein the height of the put matrix is lower than or equal to the concave surface of the plug hole;
(3) seedling culture: seedling in a rain shelter, flat ground is used as a seedbed, the length and the width of the seedbed can be determined according to the size of a plug tray, the ground condition and the like, and the daily management is convenient; a layer of sunshade net with 70% is covered on the shed roof above the seedbed; shearing robust vanilla mother vines (2 sections of growing points at the top end are removed) which are 5-10cm longer than the transverse length of a seedling culture plug tray, disinfecting cuts of the robust vanilla mother vines, placing the healthy pod vines along a U-shaped hole in the concave surface of the plug tray, clamping the mother vines in the U shape, and enabling two ends of the mother vines to respectively penetrate through round holes in two sides of the plug tray to enable the cuts at two ends of the mother vines to be exposed out of the plug tray; then slightly straightening or moving the female vine to enable each stem node of the female vine to be positioned in the hole; after the hole tray is full, spraying a rooting agent to axilla of the leaf of the mother vine stem node by using a handheld small spraying pot, after the liquid medicine is dried, filling the hole tray with coconut grains which are soaked for 8 hours and have the size of 1-3cm so as to cover the stem node, and exposing the leaf surface out of the matrix surface to complete cuttage;
(4) management: after 1-2d of cuttage, drenching a carbendazim wettable powder solution (the carbendazim wettable powder with the mass concentration of 70 percent is obtained after being diluted by 800 times), then keeping the humidity in the greenhouse at 75-85 percent, keeping the temperature at 20-30 ℃, and drenching or spraying a compound fertilizer (15-15-15) solution A with the mass concentration of 0.2 percent at the 15 th day; at 25d, removing coconut grains on stem segments of the female vines, cutting off the middle of stem segments of the female vines by using clean scissors to enable the female vines to form single (node) leaf stem segments and single hole holes, spraying a germination promoter (the germination promoter is a mixed solution of 2500 times of 24-epibrassinolide liquid with the mass concentration of 0.01% and 1500 times of gibberellic acid missible oil liquid with the mass concentration of 4%), and disinfecting the cut; after 30d, spraying the foliar fertilizer of Huabao No. 2 for 1 time at the mass concentration, and then spraying the foliar fertilizer of Huabao No. 5 for 1 time at intervals of 7-10 d;
(5) when the sprout grows to 30cm, the sprout can be outplanted or transplanted to a field for planting, or temporarily planted in a nutrition cup for culturing a big seedling; when lifting seedlings, lightly pinching two sides of the bottom of the hole, and lightly lifting the seedlings to completely take out the root system of the vanilla with the matrix; the seedling lifting and boxing of the vanilla or the boxing with the plug tray do not affect the long-distance transportation.
Example 3
A cutting propagation method of vanilla comprises the following specific operation steps:
(1) preparing a cutting medium: peat, mushroom residues (agrocybe cylindracea), garden soil and decomposed organic fertilizer are mixed according to the volume ratio of 1: 1: 0.3: 0.5, adding water, uniformly stirring until the mass water content of the substrate reaches 60-80% and the pH value is 6.0-6.5, and then composting for 15d to obtain a cuttage substrate; the decomposed organic fertilizer is obtained by fermenting organic matters with the animal excrement content of more than or equal to 45 percent (wt%) for 90 days at normal temperature;
(2) preparing a seedling raising hole tray: putting the cutting matrix obtained after retting into a seedling raising plug tray, and slightly pressing to ensure that the matrix is not loose, wherein the height of the put matrix is lower than or equal to the concave surface of the plug hole;
(3) seedling culture: seedling in a rain shelter, flat ground is used as a seedbed, the length and the width of the seedbed can be determined according to the size of a plug tray, the ground condition and the like, and the daily management is convenient; or using a greenhouse steel wire mesh seedbed to grow seedlings, and covering a layer of sunshade net with 70% of the shed roof above the seedbed; shearing robust vanilla female vines which are 5-10cm longer than the transverse length of a seedling culture plug tray (2 sections of the topmost growing point are removed), disinfecting the cuts of the robust vanilla female vines, placing the healthy vanilla female vines along a U-shaped hole in the concave surface of the plug tray, clamping the female vines in the U shape, enabling the two ends of the female vines to respectively penetrate through round holes in the two sides of the plug tray, and enabling the cuts at the two ends of the female vines to be exposed out of the plug tray; then slightly straightening or moving the female vine to enable each stem node of the female vine to be positioned in the hole; after the plug tray is full, spraying a rooting agent to axilla of the leaf of the mother vine stem node by using a handheld small spraying pot, after the liquid medicine is dried, filling the plug tray with coconut grains which are soaked for 7 hours and have the size of 1-3cm so as to cover the stem node, and exposing all the leaves out of the substrate surface to complete cuttage;
(4) management: after 1-2d of cuttage, spraying carbendazim wettable powder solution (carbendazim wettable powder with the mass concentration of 70 percent obtained after dilution by 800 times), and then keeping the humidity in the greenhouse at 75-85 percent, at the temperature of 20-30 ℃, and spraying or sprinkling compound fertilizer (15-15-15) solution with the mass concentration of 0.2 percent at the 15 th day; at the 25 th day, picking coconut grains on stem segments of the female vines, cutting off the middle of the stem segments of the female vines by using clean scissors to enable the female vines to form stem segments with single (node) leaves and single hole, spraying a germination accelerator (the germination accelerator is a mixed solution of 2500 times of 24-epibrassinolide liquid with the mass concentration of 0.01% and 1500 times of gibberellic acid missible oil liquid with the mass concentration of 4%), and disinfecting the cut; after 30d, spraying the foliar fertilizer of Huabao 5 for 1 time, and then spraying the foliar fertilizer of Huabao 2 for 1 time at intervals of 7-10 d;
(5) when the sprout grows to 30cm, the sprout can be outplanted or transplanted to a field for planting, or temporarily planted in a nutrition cup for culturing a big seedling; when seedlings are lifted, the two sides of the bottom of the hole are slightly pinched, and the roots of the vanilla with the matrix can be completely taken out by slightly lifting the seedlings; the seedling lifting and boxing of the vanilla or the boxing with the hole tray do not affect the long-distance transportation.
The method has the advantages that the mother vines are inserted into the plug tray for seedling, large-scale seedling can be carried out, the production cost is reduced, after each stem node of the mother vines is induced to root, internodes are cut off, and then germination is promoted, cut infection diseases can be reduced, the diseases are reduced, early sprouting of the stem nodes is promoted, and the sprouts are tidy; one mother vine can be divided into a plurality of cutting seedlings with one leaf and one bud, so that the seedling emergence number and the seedling rate of the mother spikes in unit length are improved; the single plant cutting seedlings with one leaf, one bud and one hole formed by the hole tray cutting and segmented seedling raising have soil for seedling raising, the root system is not easy to damage, and the method is favorable for storage, packaging and transportation or temporary planting and culturing large seedlings; the method is simple and easy to operate and can be popularized and applied in a large scale.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (9)
1. A cutting propagation method of vanilla is characterized by comprising the following operation steps:
(1) preparing a cutting medium;
(2) preparing a seedling raising plug tray;
(3) seedling culture: cutting a mother vine of vanilla pod which is 5-10cm longer than the transverse length of the seedling raising plug tray, sterilizing the cut of the mother vine, placing the sterilized mother vine along a U-shaped hole on the concave surface of the plug tray to clamp the mother vine in the U-shaped hole, and then respectively enabling the two ends of the mother vine to pass through round holes on the two sides of the plug tray to enable the cut at the two ends of the mother vine to be exposed out of the plug tray; then straightening or moving the female vines to enable each stem node of the female vines to be positioned in the hole; after the hole tray is full, spraying a rooting agent to axilla of the leaf of the stem node of the mother vine, after the liquid medicine is dried, scattering coconut grains to fill the hole tray to cover the stem node, and exposing all the leaves out of the matrix surface to finish cuttage;
(4) management: after 1d-2d of cuttage, spraying a bactericide, keeping the humidity in the greenhouse at 75-85%, keeping the temperature at 20-30 ℃, and spraying or sprinkling a compound fertilizer solution at 15 d; at 25d, opening coconut granules on stem segments of the female vines, cutting off the middle of the stem segments of the female vines to enable the female vines to form single-leaf stem segments and single-hole holes, spraying a germination promoter, and disinfecting the cut; after 30d, spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time, and then spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time at intervals of 7-10 d;
(5) when the sprout grows to more than 30cm, it can be transplanted or outplanted.
2. The cutting propagation method of vanilla according to claim 1, characterized in that: the cuttage substrate in the step (1) is prepared by mixing peat, mushroom residue, garden soil and decomposed organic fertilizer according to a volume ratio of 1: 1: 0.3: 0.5, adding water, uniformly stirring until the water content of the substrate reaches 60-80 percent and the pH value is 6.0-6.5, and then piling for more than 15 days to obtain the cuttage substrate.
3. The cutting propagation method of vanilla according to claim 2, characterized in that: the decomposed organic fertilizer is obtained by fermenting organic matters with the animal manure content of more than or equal to 45% for 90 days at normal temperature; the mushroom residue is Pleurotus Ostreatus, Pleurotus pulmonarius, and Agrocybe aegerita.
4. The cutting propagation method of vanilla according to claim 1, characterized in that: and (3) preparing the seedling raising plug tray in the step (2), namely filling the cuttage matrix obtained after the stack retting into the plug tray, and pressing to ensure that the matrix is not loosened.
5. The cutting propagation method of vanilla as claimed in claim 1, characterized in that: the seedling raising hole trays in the step (2) are formed by forming a U-shaped hole between the hole trays adjacent to each other in the transverse direction, and forming the first hole and the last hole in the transverse row, namely forming a round hole facing outwards on each of two sides of each transverse row.
6. The cutting propagation method of vanilla according to claim 1, characterized in that: the coconut grains in the step (3) are coconut grains soaked for 6-8 hours and have the size of 1-3 cm.
7. The cutting propagation method of vanilla according to claim 1, characterized in that: the bactericide in the step (4) is obtained by diluting carbendazim wettable powder solution by 800 times with 70% carbendazim wettable powder by mass concentration; in the 15 th time, spraying or spraying a compound fertilizer (15-15-15) solution A with the mass concentration of 0.2%; and after 30d, spraying the compound fertilizer solution B or spraying the foliar fertilizer for 1 time, and then spraying the compound fertilizer solution or spraying the foliar fertilizer for 1 time at intervals of 7d-10 d.
8. The cutting propagation method of vanilla as claimed in claim 7, characterized in that: the foliar fertilizer B is a compound fertilizer (15-15-15) solution with the mass concentration of 0.2% -0.5%, and the foliar fertilizers are foliar fertilizers of Huabao 2 and 5.
9. The cutting propagation method of vanilla according to claim 1, characterized in that: the bud promoting agent in the step (4) is a mixed solution of 2500 times of 24-epibrassinolide liquid with the mass concentration of 0.01% and 1500 times of gibberellic acid missible oil liquid with the mass concentration of 4%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115152767A (en) * | 2022-08-19 | 2022-10-11 | 硅羿科技（上海）有限公司 | Pesticide preparation containing 24-epibrassinolide and gibberellic acid, preparation method and application |
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| CN108575357A (en) * | 2018-04-11 | 2018-09-28 | 中国科学院武汉植物园 | More squama vanilla cuttage breeding methods |
| US20200068817A1 (en) * | 2017-02-10 | 2020-03-05 | Pompona Project Llc | Methods for cultivating vanilla pompona and methods of use thereof |
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| US20200068817A1 (en) * | 2017-02-10 | 2020-03-05 | Pompona Project Llc | Methods for cultivating vanilla pompona and methods of use thereof |
| CN108575357A (en) * | 2018-04-11 | 2018-09-28 | 中国科学院武汉植物园 | More squama vanilla cuttage breeding methods |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115152767A (en) * | 2022-08-19 | 2022-10-11 | 硅羿科技（上海）有限公司 | Pesticide preparation containing 24-epibrassinolide and gibberellic acid, preparation method and application |
| CN115152767B (en) * | 2022-08-19 | 2024-03-15 | 硅羿科技（上海）有限公司 | Pesticide preparation containing 24-epibrassinolide and gibberellic acid, and preparation method and application thereof |
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Application publication date: 20220722 Assignee: Nanning Chaohui Flower and Wood Farm Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046293 Denomination of invention: A Method for Cutting Propagation of Vanilla Granted publication date: 20230929 License type: Common License Record date: 20231108 |