CN114344213A - Skin care product containing plant extract, composition, preparation method and application thereof - Google Patents

Abstract

The invention discloses a natural plant skin care product, a composition, a preparation method and an application thereof with the functions of whitening, lightening, anti-radiation and photoaging, and the composition comprises the following components in terms of mass ratio: ethanol 1-30 parts of extract, 1-30 parts of Tanggut rhubarb leaf extract, 1-30 parts of Tanggut rhubarb rhizome ethanol extract, and 1-30 parts of arbutin. The skin care composition provided by the invention can effectively reduce melanin deposition, prevent ultraviolet damage, and improve skin resistance.

Description

A kind of skin care product containing plant extract, composition and preparation method and application thereof

technical field

The invention relates to the field of skin care products, in particular to a skin care product containing plant extracts, a composition, and a preparation method and application thereof, which have the functions of reducing melanin pigmentation, preventing ultraviolet damage and improving skin resistance.

Background technique

Moderate ultraviolet radiation in the solar spectrum can help kill bacteria on the surface of the skin and promote the formation of vitamin D. However, excessive ultraviolet radiation not only sunburns human skin, resulting in melanin and pigmentation, but also long-term direct exposure to the sun is more likely to cause skin damage and lead to Inflammation can even induce cancer and endanger human health. Ultraviolet wavelengths are strong at high altitudes and high in the sky. Some data show that the ultraviolet radiation intensity increases by about 5% for every 1,000 meters of altitude. It not only produces a large amount of tyrosine in the deep skin, causing melanin pigmentation, making the complexion dark and dark, but also reducing the skin's immunity, accelerating the aging speed, and breeding spots and wrinkles. With the continuous improvement of people's living standards, the average lifespan is getting longer and longer, and the pursuit of appearance is getting higher and higher, so the understanding of ultraviolet rays is getting deeper and deeper, and more and more people choose skin care products to reduce melanin pigmentation, prevent UV damage, improve skin resistance, whiten and fade spots, delay aging.

At present, there are a wide variety of skin-whitening, anti-radiation and anti-radiation skin care products on the market, but the curative effects are mixed. Some products are prepared from synthetic chemicals. If they are used for a long time or the dosage reaches a certain amount, it is easy to cause skin intolerance and chemical poisoning. If it does not reach a certain amount or a certain period, it is difficult to produce obvious curative effect, let alone durability. For various reasons, according to surveys, pharmaceutical skin care products are more popular with modern people, and skin care products made from pure natural ingredients are safer and more durable than skin care products made from synthetic chemicals. Therefore, natural plant skin care products with high safety, long-lasting efficacy, good whitening and anti-radiation effects, can effectively reduce melanosis, prevent ultraviolet damage, and improve skin resistance are the current research hotspots in the field of skin care products. R&D value.

SUMMARY OF THE INVENTION

The main technical problem to be solved by the present invention is to provide a natural plant-containing skin care product, a skin care composition and a preparation method of the composition, which have the functions of whitening, lightening, anti-radiation and photoaging. The skin care composition can effectively reduce melanin pigmentation, prevent ultraviolet damage, and improve skin resistance, and can be used for the preparation of various types of skin care products.

In order to solve the above-mentioned technical problems, the technical scheme adopted in the present invention is:

In the present invention, after the four components of the ethanol extract of M. spinosa, the leaf extract of rhubarb tanggut, the ethanolic extract of rhubarb rhubarb, and arbutin are combined, the whitening, spot-lightening, anti-radiation and photoaging effects are better than any A single component fully reflects the synergistic effect of the combination of these four components.

Provided is a skin care composition containing plant extracts, comprising the following components in parts by mass: 1-30 parts of Echinacea spiny extract, 1-30 parts of tangute rhubarb leaf extract, 1 part of tanggut rhubarb rhizome ethanol extract -30 parts, 1-30 parts of arbutin; the tangute rhubarb leaf extract is selected from the juice of fresh leaves.

For the purpose of extracting polysaccharides, various amino acids, vitamins, free anthraquinones and other components in the leaves of rhubarb tanggut, the extract of rhubarb tanggut leaves can also be water or ethanol extracts of the leaves.

Further, it includes the following components in parts by mass: 4-25 parts of ethanol extract of Elephant thorn, 4-25 parts of Tanggut rhubarb juice, 4-25 parts of Tanggut rhubarb rhizome ethanol extract, 4-25 parts of arbutin share.

Further, it includes the following components in parts by mass: 4-18 parts of ethanol extract of Elephant thorn, 4-18 parts of Tanggut rhubarb juice, 4-18 parts of Tanggut rhubarb rhizome ethanol extract, 13-18 parts of arbutin share.

Further, it includes the following components in parts by mass: 12 parts of ethanol extract of Meconopsis spinosa, 8 parts of Tanggut rhubarb juice, 4 parts of Tanggut rhubarb rhizome ethanol extract, and 12 parts of arbutin.

The present invention also provides the preparation method of each component of the above-mentioned skin care composition, which mainly includes the following contents:

Preparation of ethanol extract of M. spiny: M. spiny, ethanol extraction, and the extract is obtained by removing the solvent;

Preparation of ethanol extract of rhubarb rhizome of Tanggute: rhizome of rhubarb tanggut was extracted with ethanol, and the extract was solvent removed to prepare ethanolic extract of rhizome of rhubarb tanggute.

Further, the above-mentioned ethanol mass percentage is 60% to 100%.

The above-mentioned extracting liquid is to remove the solid in the extract and retain the liquid part. The methods used include but are not limited to: filtration, centrifugation and other conventional operation means for removing solids. These means can be used alone or in combination.

Further, the removal of the solvent from the above-mentioned extract is to remove the liquid in the solution, and the technical methods include but are not limited to conventional operation means such as centrifugation, filtration, thermal distillation, atmospheric distillation, and vacuum distillation. Can be used in combination.

Further, the above-mentioned ethanol extraction refers to extraction by conventional extraction methods, and the extraction methods include but are not limited to leaching, ultrasonic extraction, thermal reflux extraction, and the like.

In the present invention, the liquid-to-material ratio, extraction time, and extraction times during extraction can be obtained according to conventional test methods and optimized.

Among them, in the process of preparing the ethanolic extract of M. spiny, in order to keep the active ingredients as much as possible, it is preferable to extract at room temperature and below during ethanol extraction.

In a specific embodiment of the present invention, each component extraction method is:

Preparation of M. spiny ethanol extract: M. spiny plant is extracted with 100% ethanol at room temperature for 6-8 days, filtered, the filtrate is concentrated under low temperature and reduced pressure, and freeze-dried to obtain;

Preparation of ethanol extract of rhubarb tangute rhizome: during the harvest period of rhubarb tangute, the rhizome of the plant was collected, dried in the shade, pulverized, sieved, the powder was extracted with 75% ethanol at 60-80 ° C under reflux, the extract was concentrated and dried, Preparation of tangut rhubarb rhizome ethanol extract;

Preparation of tanggut rhubarb leaf extract: fresh leaves of tanggut rhubarb, extract the juice and filter;

The arbutin in the composition is of cosmetic grade with a purity of more than 99%;

The skin care composition is obtained by mixing the above extract and arbutin evenly according to their mass fractions.

In the present invention, Rhubarb Tanggut is a characteristic resource in Qinghai Province, and the leaves of Rhubarb Rhubarb are rich in polysaccharides, various amino acids, vitamins, free anthraquinones and other components, which can be used in skin care products to effectively resist oxidation and remove free radicals. Base, anti-inflammatory, prevent UV damage and other effects. Tanggut rhubarb rhizomes are rich in conjugated anthraquinones and other ingredients. When added to skin care products, it can inhibit the aging of epidermal cells, and have antibacterial, anti-inflammatory and anti-aging effects. Comprehensive utilization of the above-ground leaves and underground rhizomes of Tanggute rhubarb can effectively increase the utilization rate of high-quality resources and increase the added value of rhubarb.

The research of the present invention shows that the skin care composition containing plant extracts provided by the present invention has certain effects of whitening, fading, anti-radiation and photoaging, indicating that the composition provided by the present invention can be used to reduce melanin pigmentation and prevent ultraviolet rays. Skin care products that damage and improve skin resistance.

When the composition containing the plant extract provided by the present invention is used to prepare a skin care product with the effects of reducing melanosis, preventing ultraviolet damage and improving skin resistance, the mass percentage of the composition in the skin care product is 1%-27% %;

Further, the mass percentage content of the composition in the skin care product is 2%-17%;

Further, the mass percentage content of the composition in the skin care product is 5%-12%;

Further, the mass percentage content of the composition in the skin care product is 7%-10%.

The skin care composition containing plant extracts provided by the present invention can be used for the preparation of skin care products such as toners, lotions, essences, creams, facial masks, essences, gels and the like.

The present invention also provides a skin care product, which is a toner prepared from the above skin care composition and an aqueous solution of water-phase auxiliary materials, wherein the mass percentage of the skin care composition is 1-5%.

Further, the water phase excipients include but are not limited to glycerin, propylene glycol, sodium hyaluronate, and acarbomer.

In a specific embodiment of the present invention, the skin care product is a toner prepared from a skin care composition containing plant extracts and an aqueous solution of propylene glycol, wherein the mass percentage of the skin care composition is 2%.

The beneficial effects of the present invention are:

The skin care composition containing plant extracts, which has the functions of whitening, fading, anti-radiation, and photoaging, is prepared by combining the ethanol extract of Echinacea spinosa, the leaf extract of Tanggut rhubarb, the ethanol extract of Rhubarb Tanggut rhizome and bearberry. The combination of glycosides can effectively reduce melanosis, prevent UV damage, and improve skin resistance. It has been verified through experiments that the combination of each component has obvious synergistic effect, and the combined effect is better than its single effect. Pure natural ingredients, combined use is not only safe but also effective and long-lasting.

The skin care composition containing plant extracts of the present invention is screened through a large number of experiments to finally determine the optimal ratio range of the four raw materials, and based on the theory of Chinese Tibetan medicine, four natural plants are used in combination according to the optimal mass ratio. , It can achieve the effect of both internal and external adjustment on the skin, treating both the symptoms and root causes, which is safe, efficient and lasting.

The skin care composition containing plant extracts of the present invention can be applied to the preparation of skin care products of various dosage forms, can ensure the effectiveness, stability and safety of the products, and is a natural plant skin care product with whitening, lightening, anti-radiation and photoaging effects. The development is of great significance and has broad market prospects.

Detailed ways

The technical solutions of the present invention will be clearly and completely described below. Of course, the described embodiments are part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present invention. It should be pointed out that, if there are any processes that are not described in detail below, those skilled in the art can realize or understand them with reference to the prior art. If the reagents or instruments used do not indicate the manufacturer, they are regarded as conventional products that can be purchased in the market.

In specific embodiments of the present invention,

The ethanol extract of M. spiny is collected from fresh leaves of M. spiny planted in the laboratory, dried in the shade, extracted with 100% ethanol at room temperature for 7 days, filtered, and the filtrate is concentrated under low temperature and reduced pressure, and freeze-dried to obtain Echinacea spiny ethanolic extract.

Tanggut rhubarb leaf extract is to collect the fresh leaves of Tanggut rhubarb grown in the laboratory, extract the juice with a juicer, filter, and the filtrate is the Tanggut rhubarb leaf extract.

Tanggute Rhubarb Rhizome Ethanol Extract: During the harvest period of Tanggut Rhubarb, the rhizomes of the plant were collected, dried in the shade, pulverized, sieved through an 80-mesh sieve, the powder was extracted with 75% ethanol at 70 ° C under reflux, the extract was concentrated and dried, and the Tanggut rhubarb rhizome ethanolic extract was obtained.

Arbutin is cosmetic grade with a purity of over 99%.

Example 1

A skin-care composition containing plant extracts with whitening, light-spot, anti-radiation and photoaging effects, the composition is prepared from the following components by mass ratio: The mass ratio of yellow leaf extract, tangut rhubarb rhizome ethanol extract and arbutin was 1:1:1:1. Mix the above components evenly.

Example 2

A skin-care composition containing plant extracts with whitening, light-spot, anti-radiation and photoaging effects, the composition is prepared from the following components by mass ratio: The mass ratio of yellow leaf extract, tangut rhubarb rhizome ethanol extract and arbutin was 6:5:4:8. Mix the above components evenly.

Example 3

A skin care composition containing plant extracts with whitening, light-spot, anti-radiation and photoaging effects, the composition is prepared from the following components by mass ratio: The mass ratio of yellow leaf extract, tangut rhubarb rhizome ethanol extract and arbutin was 3:2:1:3. Mix the above components evenly.

Example 4

A skin care composition containing plant extracts with whitening, light-spot, anti-radiation and photoaging effects, the composition is prepared from the following components by mass ratio: The mass ratio of yellow leaf extract, tangut rhubarb rhizome ethanol extract and arbutin was 9:5:4:9. Mix the above components evenly.

Example 5

A skin care composition containing plant extracts with whitening, light-spot, anti-radiation and photoaging effects, the composition is prepared from the following components by mass ratio: The mass ratio of yellow leaf extract, tangut rhubarb rhizome ethanol extract and arbutin was 7:5:5:9. Mix the above components evenly.

Example 6

A skin care composition containing plant extracts with the functions of whitening, lightening, anti-radiation and photoaging, the composition is prepared from the following components by mass ratio: The mass ratio of extra rhubarb leaf extract, tangute rhubarb rhizome ethanol extract and arbutin was 6:5:5:9. Mix the above components evenly.

The following is a test example to explore the influence of the plant skin care combination of the present invention on the zebrafish UV damage model, to verify the UV damage prevention effect of the present invention, and the synergistic effect of each component therein:

Test Example 1

Experimental Materials

The ethanolic extract of M. spinosa, the leaf extract of rhubarb tanggut, the ethanolic extract of rhizome of rhubarb tanggut, and the skin care composition of arbutin prepared in Examples 1-6 of the present invention are all formulated into 1% by mass concentration of The solution was prepared for use, and the solvent was an aqueous DMSO solution (in the aqueous solution, the concentration of DMSO was 1%).

experimental method

Take 360 3dpf zebrafish and divide them into 12 groups with 30 tails in each group. The 12 groups were named as blank group, model group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, and ethanol extract of M. spinosa group, tanggut rhubarb leaf extract group, tanggut rhubarb rhizome ethanol extract group, arbutin group.

The zebrafish were reared in aqueous solutions of different extracts for 3 d, and the solution was changed every 12 h. The feeding water of blank group and model group is 1% DMSO aqueous solution, the feeding water of Example 1 group is 1% DMSO aqueous solution of the skin care composition prepared in Example 1, and the feeding water of Example 2 group is the skin care composition prepared in Example 2 The 1% DMSO aqueous solution of Example 3, the rearing water of Example 3 is the 1% DMSO aqueous solution of the skin care composition prepared in Example 3, the rearing water of Example 4 is the 1% DMSO aqueous solution of the skin care composition prepared in Example 4, Example The rearing water of the 5 groups is the 1% DMSO aqueous solution of the skin care composition prepared in Example 5, the rearing water of the embodiment 6 group is the 1% DMSO aqueous solution of the skin care composition prepared in Example 6, and the Echinacea spiny ethanol extract group is reared The water is firstly dissolving the ethanol extract of M. spiny in DMSO to make a mother solution, and then dissolving the mother solution in water to make an aqueous solution with a concentration of 1%. The extract is dissolved in DMSO to make mother liquor, and then the mother liquor is dissolved in water to make an aqueous solution with a concentration of 1%. The feeding water of the ethanol extract of Rhubarb tanguticum rhizome is dissolved in DMSO to make the mother liquor. The mother liquor was dissolved in water to prepare an aqueous solution with a concentration of 1%. The feeding water of the arbutin group was arbutin dissolved in DMSO to prepare a mother liquor, and then the mother liquor was dissolved in water to prepare an aqueous solution with a concentration of 0.02%. According to literature records and preliminary tests, 0.02% arbutin can effectively inhibit the formation of melanin in zebrafish, and can whiten and lighten spots.

After 3 days of administration, the zebrafish were anesthetized with MS-222, placed under a stereo microscope, and the head and chest were photographed to record the melanin formation area of each zebrafish in each group.

After taking pictures, preheat the UV cross-linking apparatus for 20 min, and when the intensity is stable, place the culture dish in the UV cross-linking apparatus (305 nm), and irradiate it for 30 s every half an hour within 3 h. 2 hours after the last irradiation, the caudal fin damage of zebrafish was observed under a stereoscopic fluorescence microscope.

statistical methods

表示，组间比较用单因素方差分析。After taking pictures under the stereoscopic fluorescence microscope, ImageJ software analyzed the area of melanin in the cephalothorax and the caudal fin. The experimental data were analyzed by SPSS 21.0 statistical software.

The comparison between groups was performed by one-way ANOVA.

result

Table 1 shows the effect of each skin care composition of the present invention on the area of zebrafish melanin formation.

)Table 1 The area of zebrafish melanin formation in each group (n=30,

)

The experimental results in Table 1 show that compared with the blank group, the area of zebrafish melanin formation is significantly reduced, and each combination can inhibit the formation of melanin to varying degrees and effectively lighten the spots. Examples 1 to 6 groups of products and the ethanol extract group of Meconopsis thorn, the tangute rhubarb leaf extract group, the tangute rhubarb rhizome ethanol extract and the arbutin group all have a certain effect of inhibiting the formation of pigmentation spots. Examples 3 and 6 have the most obvious effect, and there is a significant difference compared with the blank group.

Table 2 shows the effect of the skin care composition of the present invention on the area unit of the caudal fin of the zebrafish UV radiation damage model.

)Table 2 The area content of zebrafish tail fin in each group (n=30,

)

The experimental results in Table 2 show that compared with the blank group, the area of the caudal fin of the zebrafish in the model group was significantly reduced, indicating that ultraviolet radiation caused serious damage to the zebrafish epidermis. Examples 1 to 6 groups of products and the ethanolic extract of Elephant thorns, the leaf extract of Rhubarb Tanggut, the ethanolic extract of Rhubarb Rhubarb, and the arbutin group all have a certain effect of preventing ultraviolet damage, wherein the embodiment 2, 3, 4, and 6 have stronger effects, and there is a significant difference compared with the model group, especially the synergistic effect of the products of Examples 2 and 3 is obvious.

Test Example 2 Clinical Trial

The natural plant skin care composition of the present invention was clinically observed and studied. Taking the combination of Example 3 as an example, the skin care composition of Example 3 was dissolved in an aqueous propylene glycol solution to prepare a 2% mass fraction of skin care water. A total of 22 volunteers with skin problems (all with informed consent) were included, ranging in age from 18 to 45 years old, including 8 males and 14 females. The botanical skin care combination skin lotion was tested for a total of 4 weeks. For the analysis of skin problems and treatment effects, the statistics are shown in Table 2.

Table 3 Clinical trial results

From the results, it can be seen that the skin care lotion made from the natural plant skin care combination with whitening and lightening effect provided in Example 3 has a significant therapeutic effect on lightening dark spots, whitening and brightening, and preventing aging of facial skin, and also has moisturizing effects. Moisturizing and reducing fine lines.

To sum up, the combination of the components in the plant skin care combination of the present invention has a synergistic effect, the plant skin care combination of the present invention has significant freckle removal effect, effective anti-ultraviolet radiation, and each component has no toxic side effects and high safety , can significantly improve skin quality.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, and substitutions can be made in these embodiments without departing from the principle and spirit of the invention and modifications, the scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

1. A skin care composition containing plant extracts is characterized by comprising the following components in parts by mass: 1-30 parts of artemisia torrifolia ethanol extract, 1-30 parts of rheum tanguticum leaf extract, 1-30 parts of rheum tanguticum rhizome ethanol extract and 1-30 parts of arbutin; the Rheum tanguticum Maxim leaf extract is selected from juice of fresh leaves, or water extract or ethanol extract of leaves.

2. The composition according to claim 1, which is characterized by comprising the following components in parts by mass: 4-25 parts of artemisia pluvialis ethanol extract, 4-25 parts of rheum tanguticum leaf extract, 4-25 parts of rheum tanguticum rhizome ethanol extract and 4-25 parts of arbutin.

3. The skin care composition according to claim 1, which comprises the following components in parts by mass: 4-18 parts of artemisia torrifolia ethanol extract, 4-18 parts of rheum tanguticum leaf extract, 4-18 parts of rheum tanguticum rhizome ethanol extract and 4-18 parts of arbutin; further, the paint comprises the following components in parts by mass: 12 parts of artemisia pluvialis ethanol extract, 8 parts of rheum tanguticum leaf extract, 4 parts of rheum tanguticum rhizome ethanol extract and 12 parts of arbutin.

4. A method of preparing a skin care composition according to any of claims 1 to 3 comprising the steps of:

preparing the artemisia scoparia ethanol extract: extracting herba Artemisiae Halodendri Spreng with ethanol, and removing solvent from the extractive solution;

preparing the rheum tanguticum rhizome ethanol extract: extracting the rhizome of the rhubarb from the Tangut, and removing the solvent from the extract to prepare the ethanol extract of the rhizome of the rhubarb from the Tangut.

5. The method according to claim 4, wherein the ethanol is used in an amount of 60 to 100% by mass.

6. Use of a plant extract-containing skin care composition according to any one of claims 1 to 3 for the preparation of a natural plant skin care product for reducing melanin pigmentation, preventing uv damage, and improving skin resistance.

7. The use of the plant extract skin care composition in a skin care product according to claim 5, wherein the mass percentage of the skin care composition in the skin care product is 1-27%; further, the skin care composition accounts for 7-10% of the skin care product by mass.

8. The use of the botanical extract skin care composition as claimed in claim 5 in skin care products, wherein the natural botanical skin care product topical product is a toner, lotion, essence, cream, mask, essence, gel.

9. A skin care product containing plant extracts is characterized in that the skin care product is toner prepared from the skin care composition as claimed in any one of claims 1 to 4 and an aqueous phase auxiliary material solution, wherein the mass percentage of the skin care composition is 1-5%.

10. The skin care product of claim 8, wherein the water phase adjuvant is one or more of glycerin, propylene glycol, sodium hyaluronate and carbomer.