CN1142280C - 改进的蛋白质原核表达 - Google Patents
改进的蛋白质原核表达 Download PDFInfo
- Publication number
- CN1142280C CN1142280C CNB988045311A CN98804531A CN1142280C CN 1142280 C CN1142280 C CN 1142280C CN B988045311 A CNB988045311 A CN B988045311A CN 98804531 A CN98804531 A CN 98804531A CN 1142280 C CN1142280 C CN 1142280C
- Authority
- CN
- China
- Prior art keywords
- wpra
- gene
- protein
- bacillus subtillis
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9708452.9 | 1997-04-26 | ||
GBGB9708452.9A GB9708452D0 (en) | 1997-04-26 | 1997-04-26 | Improved prokaryotic expression of protein |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1253591A CN1253591A (zh) | 2000-05-17 |
CN1142280C true CN1142280C (zh) | 2004-03-17 |
Family
ID=10811386
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB988045311A Expired - Fee Related CN1142280C (zh) | 1997-04-26 | 1998-04-24 | 改进的蛋白质原核表达 |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0977875B1 (de) |
JP (1) | JP4312834B2 (de) |
CN (1) | CN1142280C (de) |
AT (1) | ATE353971T1 (de) |
AU (1) | AU7057098A (de) |
DE (1) | DE69837086T2 (de) |
DK (1) | DK0977875T3 (de) |
GB (1) | GB9708452D0 (de) |
WO (1) | WO1998049328A1 (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100411238B1 (ko) * | 2000-08-19 | 2003-12-18 | 한국과학기술원 | 외래 단백질을 안정하게 발현하는 바실러스 속 미생물 |
CN1659283A (zh) | 2002-04-10 | 2005-08-24 | 诺维信公司 | 地衣芽孢杆菌的突变宿主细胞 |
AU2003214036A1 (en) * | 2002-04-10 | 2003-10-27 | Novozymes A/S | Bacillus licheniformis mutant host cell |
ATE447031T1 (de) | 2004-04-07 | 2009-11-15 | Novozymes As | Mutierte prokaryontische zellen mit hohen sekretionsniveaus |
JP4485341B2 (ja) * | 2004-12-20 | 2010-06-23 | 花王株式会社 | 組換え微生物 |
US7981659B2 (en) | 2005-10-13 | 2011-07-19 | Kao Corporation | Bacillus subtilis mutant strain |
AR100605A1 (es) | 2014-05-27 | 2016-10-19 | Novozymes As | Métodos para la producción de lipasas |
EP3233893B1 (de) * | 2014-12-16 | 2021-01-20 | Danisco US Inc. | Erhöhte protein expression |
EP3615659A4 (de) | 2017-04-28 | 2021-01-06 | Agrospheres, Inc. | Zusammensetzungen und verfahren zur verkapselung und skalierbaren abgabe von agrochemikalien |
WO2019060903A1 (en) | 2017-09-25 | 2019-03-28 | Agrospheres, Inc. | COMPOSITIONS AND METHODS FOR PRODUCING AND EVOLVING ADMINISTRATION OF BIOLOGICAL PRODUCTS |
CN110592125B (zh) * | 2019-09-30 | 2023-09-01 | 南京农业大学 | 一种食品级降解乙醇枯草杆菌重组菌的构建方法 |
CN113025601B (zh) * | 2019-12-25 | 2024-06-21 | 上海奥博生物医药股份有限公司 | 腈水解酶启动子优化表达及应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2003078A1 (en) * | 1988-11-18 | 1990-05-18 | Alan Sloma | Protease deletion |
US5294542A (en) * | 1991-03-19 | 1994-03-15 | Omnigene, Inc. | Residual protease-III |
-
1997
- 1997-04-26 GB GBGB9708452.9A patent/GB9708452D0/en active Pending
-
1998
- 1998-04-24 WO PCT/GB1998/001051 patent/WO1998049328A1/en active IP Right Grant
- 1998-04-24 AT AT98917325T patent/ATE353971T1/de not_active IP Right Cessation
- 1998-04-24 EP EP98917325A patent/EP0977875B1/de not_active Revoked
- 1998-04-24 DE DE69837086T patent/DE69837086T2/de not_active Expired - Fee Related
- 1998-04-24 AU AU70570/98A patent/AU7057098A/en not_active Abandoned
- 1998-04-24 DK DK98917325T patent/DK0977875T3/da active
- 1998-04-24 JP JP54669998A patent/JP4312834B2/ja not_active Expired - Fee Related
- 1998-04-24 CN CNB988045311A patent/CN1142280C/zh not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE69837086T2 (de) | 2007-11-15 |
CN1253591A (zh) | 2000-05-17 |
WO1998049328A1 (en) | 1998-11-05 |
GB9708452D0 (en) | 1997-06-18 |
DE69837086D1 (de) | 2007-03-29 |
EP0977875B1 (de) | 2007-02-14 |
DK0977875T3 (da) | 2007-06-04 |
EP0977875A1 (de) | 2000-02-09 |
JP2001527401A (ja) | 2001-12-25 |
ATE353971T1 (de) | 2007-03-15 |
AU7057098A (en) | 1998-11-24 |
JP4312834B2 (ja) | 2009-08-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB02 | Change of applicant information |
Co-applicant after: Novo Nordisk A/S Co-applicant before: Novo Nordisk A/S |
|
COR | Change of bibliographic data |
Free format text: CORRECT: CO-APPLICANT; FROM: NOVO NORDISK A/S TO: NOVOZYMES COMPANY |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: NEWCASTLE UNIVERSITY MERCHANDISE CO., LTD. Free format text: FORMER OWNER: NEWCASTLE UNIVERSITY |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20050311 Address after: The British in Newcastle upon Tyne Co-patentee after: Novo Nordisk A/S Patentee after: University of Newcastle commodities Ltd Address before: The British in Newcastle upon Tyne Co-patentee before: Novo Nordisk A/S Patentee before: Newcastle University |
|
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20040317 Termination date: 20100424 |