CN1121355A - 胆盐刺激的脂酶变异体、编码其之dna分子和转基因非人类哺乳动物 - Google Patents
胆盐刺激的脂酶变异体、编码其之dna分子和转基因非人类哺乳动物 Download PDFInfo
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Abstract
Description
寡核苷酸 | 序列(5′-3′) |
PCR-1 | CGGGATCCGAAGCCCTTCGCCACCCCCACG |
PCR-2 | CGAAGCTTGTCGACTTACTACTGATCAGTCACTGTGGGCAGCGCCAG |
PCR-3 | GGGAATTCTGGCCATTGCTTGGGTGAAGAGGAATATCGCGGCCTTCGGGGGGGACCCCAACCAGATCACGCTCTTCGGGGAGTCT* |
PCR-4 | CGGGATCCCACATACTGCAGCATGGGGTACTCCAGGCC |
1 | GATCAGGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGG |
2 | GCCCCCCCCGTGCCGCCCACGGGTGACTCCAAGGAAGCTCAGA |
3 | TGCCTGCAGTCATTAGGTTTTAGTAAGTCGACA |
4 | AGCTTGTCGACTTACTAAAACCTAATGACTG |
5 | CAGGCATCTGAGCTTCCTTGGAGTCACCCGTGGGCGGCACGGGGGGGGCCCCGGA |
6 | GTCACCCGTGGGCGGCACGGGGGGGGCCCCCT |
7 | GATCAGAAGGAAGCTCAGA |
8 | CAGGCATCTGAGCTTCCTTCT |
变异体 | 缺失的残基 | 变化的残基 |
A(SEQ ID NO:4) | 536-722 | |
B(SEQ ID NO:5) | 536-711 | |
C(SEQ ID NO:6) | 536-568,591-711 | |
N(SEQ ID NO:7) | Asn 187→Gln |
酶 | Mr (KDa)通过SDS-PAGE测定 |
全 长 | 105,107 |
变异体B | 63,65 |
变异体C | 60,62 |
变异体N | 95 |
质粒 | 保藏号 | 保藏日 |
pS309pS310pS311pS317 | DSM 7101DSM 7102DSM 7103DSM 7104 | 1992.6.12 |
pS147pS257pS299 | DSM 7495DSM 7496DSM 7497 | 1993.2.26 |
pS258pS259 | DSM 7501DSN 7502 | 1993.3.3 |
Claims (42)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9300686A SE9300686D0 (sv) | 1993-03-01 | 1993-03-01 | Novel polypeptides |
SE9300686-4 | 1993-03-01 | ||
SE9300722-7 | 1993-03-04 | ||
SE9300722A SE9300722D0 (sv) | 1993-03-04 | 1993-03-04 | Novel polypeptides ii |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB001377388A Division CN1187450C (zh) | 1993-03-01 | 1994-02-25 | 生产转基因非人类哺乳动物的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1121355A true CN1121355A (zh) | 1996-04-24 |
CN1071790C CN1071790C (zh) | 2001-09-26 |
Family
ID=26661673
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN94191848A Expired - Lifetime CN1071790C (zh) | 1993-03-01 | 1994-02-25 | 胆盐刺激的脂酶变异体、其制法、用途及编码它的核酸 |
CNB001377388A Expired - Lifetime CN1187450C (zh) | 1993-03-01 | 1994-02-25 | 生产转基因非人类哺乳动物的方法 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB001377388A Expired - Lifetime CN1187450C (zh) | 1993-03-01 | 1994-02-25 | 生产转基因非人类哺乳动物的方法 |
Country Status (28)
Country | Link |
---|---|
US (2) | US5827683A (zh) |
EP (1) | EP0687296B1 (zh) |
JP (1) | JP3837444B2 (zh) |
KR (2) | KR100312825B1 (zh) |
CN (2) | CN1071790C (zh) |
AT (1) | ATE317429T1 (zh) |
AU (1) | AU675701B2 (zh) |
BR (1) | BR9406376A (zh) |
CA (1) | CA2156083C (zh) |
CZ (1) | CZ290927B6 (zh) |
DE (1) | DE69434622T2 (zh) |
DK (1) | DK0687296T3 (zh) |
EE (1) | EE9400458A (zh) |
ES (1) | ES2258262T3 (zh) |
FI (1) | FI954082A0 (zh) |
HU (1) | HU221119B1 (zh) |
IL (2) | IL108698A0 (zh) |
IS (1) | IS4130A (zh) |
NO (1) | NO953426L (zh) |
NZ (1) | NZ262529A (zh) |
PL (1) | PL184960B1 (zh) |
PT (1) | PT687296E (zh) |
RU (1) | RU2219239C2 (zh) |
SG (1) | SG52597A1 (zh) |
SK (1) | SK285420B6 (zh) |
TW (1) | TW387013B (zh) |
UA (1) | UA48109C2 (zh) |
WO (1) | WO1994020610A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103562383A (zh) * | 2011-05-18 | 2014-02-05 | 瑞典孤儿比奥维特鲁姆有限公司 | 低pH蛋白质纯化方法 |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5681819A (en) * | 1994-12-01 | 1997-10-28 | Oklahoma Medical Research Foundation | Method and compositions for reducing cholesterol absorption |
US5821226A (en) * | 1994-12-01 | 1998-10-13 | Oklahoma Medical Research Foundation | BAL C-tail drug delivery molecules |
US5696087A (en) * | 1994-12-01 | 1997-12-09 | Oklahoma Medical Research Foundation | Method and compositions for reducing cholesterol absorption |
JP3007161B2 (ja) * | 1994-12-01 | 2000-02-07 | オクラホマ メディカル リサーチ ファウンデーション | コレステロール吸収を減少するための方法および組成物 |
FR2733249B1 (fr) * | 1995-04-20 | 1997-06-06 | Biocem | Lipase gastrique de chien recombinante et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations |
SE9501939D0 (sv) * | 1995-05-24 | 1995-05-24 | Astra Ab | DNA molecules for expression of polypeptides |
US6342218B1 (en) | 1997-02-14 | 2002-01-29 | Oklahoma Medical Research Foundation | Method for treatment of SLE |
SE9801424D0 (sv) | 1998-04-22 | 1998-04-22 | Astra Ab | Expression methods |
WO2001081366A2 (en) * | 2000-04-21 | 2001-11-01 | Monsanto Technology Llc | Purification of ace inhibiting polypeptides containing vpp from milk |
FR2868424B1 (fr) * | 2004-03-31 | 2008-04-11 | Univ Aix Marseille Ii | Glycopeptides derives de structures pancreatiques, anticorps et leurs applications en diagnostic et therapeutique |
CA2558754C (fr) | 2004-03-31 | 2015-09-22 | Universite De La Mediterranee | Glycopeptides derives de structures pancreatiques, anticorps et leurs applications en diagnostic et therapeutique |
CN100343391C (zh) * | 2004-12-31 | 2007-10-17 | 中国农业大学 | 卵巢注射法制备转基因动物 |
EP2039764A1 (en) * | 2007-09-19 | 2009-03-25 | Pevion Biotech AG | Truncated secretory aspartyl proteinase 2 |
JP5850940B2 (ja) * | 2010-10-21 | 2016-02-03 | スウェディッシュ オーファン バイオビトラム パブリーク アクチエボラグ | ヒト乳児の発育速度を増大させる方法 |
RU2013123056A (ru) | 2010-10-21 | 2014-11-27 | Сведиш Орфан Биовитрум Аб (Пабл) | Способ повышения всасывания ненасыщенных жирных кислот у грудных детей |
FR2966734B1 (fr) * | 2010-10-29 | 2014-07-18 | Max Rombi | Composition comprenant au moins une enzyme proteolytique pour son utilisation pour empecher la synthese des triglycerides |
CN103088000A (zh) * | 2012-03-23 | 2013-05-08 | 北京济福霖生物技术有限公司 | 在哺乳动物乳腺中过表达胆盐激活脂酶的方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4944944A (en) * | 1987-11-19 | 1990-07-31 | Oklahoma Medical Research Foundation | Dietary compositions and methods using bile salt-activated lipase |
US5200183A (en) * | 1987-11-19 | 1993-04-06 | Oklahoma Medical Research Foundation | Recombinant bile salt activated lipases |
SE9001985D0 (sv) * | 1990-06-01 | 1990-06-01 | Astra Ab | New chemical products |
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- 1995-05-19 US US08/445,050 patent/US5763739A/en not_active Expired - Lifetime
- 1995-08-31 NO NO953426A patent/NO953426L/no not_active Application Discontinuation
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103562383A (zh) * | 2011-05-18 | 2014-02-05 | 瑞典孤儿比奥维特鲁姆有限公司 | 低pH蛋白质纯化方法 |
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