CN112005654A - Method for shortening dormancy period of paris polyphylla seeds and seedling growing method - Google Patents

Method for shortening dormancy period of paris polyphylla seeds and seedling growing method Download PDF

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Publication number
CN112005654A
CN112005654A CN202010960531.0A CN202010960531A CN112005654A CN 112005654 A CN112005654 A CN 112005654A CN 202010960531 A CN202010960531 A CN 202010960531A CN 112005654 A CN112005654 A CN 112005654A
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China
Prior art keywords
seeds
paris polyphylla
seedling
taking out
temperature
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CN202010960531.0A
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Chinese (zh)
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徐廷荣
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Huili Jiahe Agriculture Co ltd
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Huili Jiahe Agriculture Co ltd
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Priority to CN202010960531.0A priority Critical patent/CN112005654A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a seedling raising method of paris polyphylla seeds, which comprises the following steps: comprises the following steps of 1) collecting mature rhizoma paridis seeds, removing the seed skin, and naturally drying in the shade at 10-15 deg.C for 10-15 d; 2) treating the seeds in a refrigeration and normal temperature alternative mode; 3) soaking the seeds in boiled boiling water for 6-10s, taking out, and rapidly soaking in cold water; 4) taking out the seeds, soaking the seeds in 800 times of carbendazim 500-fold liquid for 2-3h, taking out and airing; 4) sowing the seeds on a seedling bed, covering a seedling substrate, and watering thoroughly. The seedling raising method can advance the memory of the paris polyphylla seeds by two dormant seasons, greatly shorten the dormant period of the paris polyphylla seeds, effectively shorten the seedling emergence period and improve the seedling emergence rate.

Description

Method for shortening dormancy period of paris polyphylla seeds and seedling growing method
Technical Field
The invention relates to the technical field of agriculture, in particular to a method for shortening a dormancy period of paris polyphylla seeds and a seedling growing method.
Background
The paris polyphylla is a traditional national medicine, is recorded in Shen nong Ben Cao Jing by the name of 'pariphyllin', and is a dry rhizome of Yunnan paris polyphylla or paris polyphylla of liliaceae by taking paris polyphylla as a formal medicine name by the national medical book 'Yunnan Ben Cao'; it is bitter in taste and slightly cold in nature; has little toxicity; has the efficacies of clearing away heat and toxic material, relieving swelling and pain, and cooling liver and arresting convulsion, and is commonly used for treating furuncle and carbuncle, swollen and sore throat, snake and insect bite, traumatic injury and convulsion.
The method for rapidly developing the planting of the paris polyphylla needs a large amount of high-quality seedlings, and the paris polyphylla seedlings are mainly bred in two modes of underground stem breeding and seed sowing and seedling breeding. The Paris polyphylla resources are less, the underground stem cutting propagation mode is long, and the propagation coefficient is low; the conventional seeds are sowed and grown, the seedlings can emerge and grow continuously only in 1-2 winter, the seedling emergence period is long and irregular, the seedling emergence rate is low, the yield of the paris polyphylla seeds is very low, and the difficulty of sowing and growing the seedlings by the conventional seeds is a main reason for the shortage of the paris polyphylla seedlings. Aiming at the defects, a method for cultivating the seedlings of the paris polyphylla is necessary.
Disclosure of Invention
In order to solve the problems, the invention provides a method for shortening the dormancy period of the paris polyphylla seeds and a seedling growing method, which can lead the memory of the paris polyphylla seeds to be advanced by two dormancy seasons, greatly shorten the dormancy period of the paris polyphylla seeds, effectively shorten the seedling emergence period and improve the seedling emergence rate.
The invention is realized by the following technical scheme:
a method for shortening a dormancy period of a paris polyphylla seed, wherein the seed treatment comprises the steps of: 1) refrigerating peeled rhizoma paridis seeds at 0-1 deg.C for 7d-10d, taking out, and standing at room temperature for 5-7 d; 2) refrigerating the paris polyphylla seeds treated in the step 1) at the temperature of 3-7 ℃ for 5d-7d, and then taking out and placing the paris polyphylla seeds at normal temperature for 3d-5 d; 3) refrigerating the paris polyphylla seeds treated in the step 2) at 1 ℃, and heating to 7 ℃ at the amplitude of 1 ℃ every day to finish the dormancy stage treatment of the seeds.
In the step 1), the refrigeration temperature of the seeds is gradually reduced from 7 ℃ in a range of 1 ℃ per day until the refrigeration is continued for 7d to 10d after the temperature reaches 0 ℃ to 1 ℃.
Before the step 3), the seeds treated in the step 2) are reduced at the normal temperature at the amplitude of 6 ℃ per day until the temperature is reduced to 0 ℃, and then the seeds are continuously refrigerated for 10d-15 d.
Before the step 3), the seeds treated in the step 2) are refrigerated for 5 days at 7 ℃ and then are placed for 1 day at room temperature.
In the step 1), the seeds are mixed with soil with 35% -45% of water content according to the proportion of 1:2, and then refrigerated.
The invention adopts a special refrigeration and normal temperature alternate treatment mode, wherein the once refrigeration and normal temperature treatment are equivalent to the situation that the seeds are subjected to a dormancy period, the dormancy period can be quickly passed by the seeds in a short time through a manual mode, the memory of the paris polyphylla seeds can be advanced by two dormancy seasons, and the dormancy period of the seeds is greatly shortened, so that the germination and emergence periods of the seeds are shortened.
A seedling raising method of Paris polyphylla seeds comprises the following steps of 1) collecting mature Paris polyphylla seeds, removing the seed skins of the Paris polyphylla seeds, and naturally drying the Paris polyphylla seeds in the shade at the temperature of 10-15 ℃ for 10-15 d; 2) treating the seeds in a refrigeration and normal temperature alternative mode; 3) soaking the seeds in boiled boiling water for 6-10s, taking out, and rapidly soaking in cold water; 4) taking out the seeds, soaking the seeds in 800 times of carbendazim 500-fold liquid for 2-3h, taking out and airing; 4) sowing the seeds on a seedling bed, covering a seedling substrate, and watering thoroughly.
And 4) mixing the seedling culture substrates of the seeds according to the mass ratio of 6:2:9 of humus, organic fertilizer and loam.
The cold water is ice blocks and water according to the mass ratio of 1: 50, and mixing.
The seeding rate of the seeds in the step 4) is 580-620 grains per square.
The seedling method adopts a cold storage and normal temperature alternate mode plus boiling water and rapid cooling treatment mode for seeds, the cold storage and the normal temperature alternate mode can shorten the dormancy period of the seeds, the seeds are treated by the boiling water, mainly, the skins of the white seeds of the paris polyphylla seeds have things like a layer of film, the soil moisture is difficult to enter after direct sowing, the seeds are cooled immediately after the boiling water treatment, and the film can crack through the principle of expansion with heat and contraction with cold, so that the seeds can be rapidly received by soil to rapidly fuse with each other in the field to sprout more rapidly, the sprouting time of the seeds can be greatly shortened, and the emergence rate of the seeds can be improved; wherein the germination time is usually 120-140 days, the germination time is 140-180 days, and the germination rate is as high as 80-85%.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. the seedling raising method of the paris polyphylla seeds adopts a special refrigeration and normal temperature alternate treatment mode, wherein one refrigeration and normal temperature treatment are equivalent to a situation that the seeds are subjected to a dormancy period, the dormancy period is quickly passed by the seeds in a short time in a manual mode, the memory of the paris polyphylla seeds can be advanced by two dormancy seasons, and the dormancy period of the seeds is greatly shortened;
2. according to the seedling raising method for the paris polyphylla seeds, the seeds are treated by boiling water and then are cooled immediately, and the thin film on the surfaces of the seeds can be broken by the principle of expansion with heat and contraction with cold, so that the seeds can be quickly fused with the ground to quickly receive water to bud, the germination time of the seeds can be greatly shortened, and the germination rate of the seeds can be improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
In the case of the example 1, the following examples are given,
a seedling raising method of rhizoma paridis seeds comprises 1) collecting mature rhizoma paridis seeds, removing seed shells, and naturally drying in shade at 10 deg.C for 15 days; 2) packaging the rhizoma paridis seeds with 2.5 kg of food bags, placing in a refrigerator, gradually lowering the temperature of the refrigerator from 7 deg.C to 1 deg.C every day until the temperature reaches 0 deg.C, placing in the refrigerator at 0 deg.C for 7 days, taking out the seeds, and standing at room temperature for 7 days; 3) gradually raising the temperature of the refrigerator at a speed of 2 ℃/day until the temperature of the refrigerator reaches 7 ℃, standing for 5 days, taking out the seeds, and standing for 5 days at normal temperature; 4) rubbing off the red skin outside the seeds, then placing the seeds in a refrigerator, reducing the temperature of the refrigerator at the range of 6 ℃ per day until the temperature is reduced to 0 ℃, and then placing the seeds in the refrigerator at 0 ℃ for 15 days; 5) gradually raising the temperature of the refrigerator at a heating speed of 1 ℃/day until the temperature of the refrigerator reaches 7 ℃; 6) soaking the seeds in boiled boiling water for 10s, taking out, and quickly soaking in cold water; 7) taking out the paris polyphylla seeds, soaking the paris polyphylla seeds in 800 times of carbendazim solution for 3 hours, taking out and airing the paris polyphylla seeds; 8) then, the seeds are sowed on a seedling bed, the soil thickness is 2 cm, and the seeds are watered thoroughly.
Finally, the seeds germinate in 140 days and emerge in 180 days in the soil, the period of emergence of the paris polyphylla seeds is shortened from two years to 6 months, the germination rate of the paris polyphylla seeds is effectively improved, and the emergence rate reaches 82%.
Example 2
A seedling raising method of rhizoma paridis seeds comprises 1) collecting mature rhizoma paridis seeds, removing seed shells, and naturally drying in shade at 15 deg.C for 10 days; 2) mixing the seeds of the paris polyphylla with soil with the water content of 35-45% according to the proportion of 1:2, and placing the mixture in a refrigerator, wherein the temperature of the refrigerator is adjusted to be 1 ℃ and the mixture is placed in the refrigerator for 10 days; taking out and standing at normal temperature for 5 days; 3) placing the seeds in a refrigerator at 3 ℃ for 7 days, taking out the seeds and placing the seeds at normal temperature for 3 days; 4) placing the seeds in a refrigerator at 7 ℃ for 5 days, taking out the seeds and placing the seeds at normal temperature for 1 day; 5) gradually raising the temperature of the refrigerator at a heating speed of 1 ℃/day until the temperature of the refrigerator reaches 7 ℃; 6) soaking the seeds in boiled boiling water for 6s, taking out, and quickly soaking in cold water; 7) taking out the paris polyphylla seeds, soaking the paris polyphylla seeds in 500 times of carbendazim solution for 2 hours, taking out the paris polyphylla seeds and airing the paris polyphylla seeds; 8) then, the seeds are sowed on a seedling bed, the soil thickness is 2 cm, and the seeds are watered thoroughly.
Finally, the seeds germinate in 120 days and emerge in 140 days in the soil, the period of emergence of the paris polyphylla seeds is shortened to 5 months from two years, the germination rate of the paris polyphylla seeds is effectively improved, and the emergence rate reaches 85%.
Comparative example
A traditional seedling raising method is different from the examples 1 and 2 in that the seeds are not subjected to the refrigeration and boiling water treatment of the steps 2) to 6), the seedling emergence time is different from 300-.
Compared with the traditional seedling raising method, the seed treatment and cultivation method can effectively shorten the seedling emergence period, improve the seedling emergence rate, ensure the uniform seedling emergence, ensure the germination time to be only about 4-5 months and ensure the highest seedling emergence rate to be 80-85%.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (9)

1. A method for shortening the dormancy period of paris polyphylla seeds, wherein the seed treatment comprises the steps of: 1) refrigerating peeled rhizoma paridis seeds at 0-1 deg.C for 7d-10d, taking out, and standing at room temperature for 5-7 d; 2) refrigerating the paris polyphylla seeds treated in the step 1) at the temperature of 3-7 ℃ for 5d-7d, and then taking out and placing the paris polyphylla seeds at normal temperature for 3d-5 d; 3) refrigerating the paris polyphylla seeds treated in the step 2) at 1 ℃, and heating to 7 ℃ at the amplitude of 1 ℃ every day to finish the dormancy stage treatment of the seeds.
2. The method of claim 1, wherein the refrigeration temperature of the seeds in step 1) is gradually decreased from 7 ℃ to 1 ℃ per day until the temperature reaches 0-1 ℃ and then the refrigeration is continued for 7d-10 d.
3. The method for shortening dormancy period of Paris polyphylla seeds of claim 1, wherein before step 3), the seeds treated in step 2) are cooled down at 6 ℃ per day at normal temperature until the temperature is lowered to 0 ℃ and then kept for 10d-15 d.
4. The method for shortening dormancy period of Paris polyphylla seeds of claim 1, wherein the seeds treated in step 2) are refrigerated at 7 ℃ for 5 days before step 3), and then kept at room temperature for 1 day.
5. The method for shortening the dormancy period of the paris polyphylla seeds of claim 1, wherein in step 1), the seeds are mixed with soil with 35% -45% of water content according to the proportion of 1:2 and then refrigerated.
6. A seedling raising method of paris polyphylla seeds is characterized by comprising the following steps of 1) collecting mature paris polyphylla seeds, removing seed skins of the paris polyphylla seeds, and naturally drying the paris polyphylla seeds in the shade at 10-15 ℃ for 10-15 d; 2) treating the seed according to any one of claims 1 to 5; 3) soaking the seeds in boiled boiling water for 6-10s, taking out, and rapidly soaking in cold water; 4) taking out the seeds, soaking the seeds in 800 times of carbendazim 500-fold liquid for 2-3h, taking out and airing; 4) sowing the seeds on a seedling bed, covering a seedling substrate, and watering thoroughly.
7. A method for growing seedlings of paris polyphylla seeds as claimed in claim 6, wherein in step 4), the seedling-growing substrates of the seeds are mixed according to the mass ratio of humus soil, organic fertilizer and loam soil to be 6:2: 9.
8. The method for raising seedlings of paris polyphylla seeds of claim 6, wherein the cold water is ice cubes and water in a mass ratio of 1: 50, and mixing.
9. The method as claimed in claim 6, wherein the seeding amount of the seeds in step 4) is 580-620 grains per square.
CN202010960531.0A 2020-09-14 2020-09-14 Method for shortening dormancy period of paris polyphylla seeds and seedling growing method Pending CN112005654A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6449899B1 (en) * 2001-03-08 2002-09-17 Council Of Scientific And Industrial Research Method for inducing improved seed germination in Podophyllum hexandrum Royle
CN101189943A (en) * 2006-11-18 2008-06-04 怀化医学高等专科学校 Seed propagating fast breeding method for paris polyphylla
CN102835207A (en) * 2012-09-19 2012-12-26 贵州三元太宝实业股份有限公司 Method for reducing sleeping cycle of Paris polyphylla seeds
CN104541655A (en) * 2013-10-15 2015-04-29 云南白药集团中药材优质种源繁育有限责任公司 Method for breaking paris polyphylla seed dormancy
CN110089378A (en) * 2019-05-24 2019-08-06 成都市农林科学院 A kind of efficient Paris polyphylla seed seedling-raising method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6449899B1 (en) * 2001-03-08 2002-09-17 Council Of Scientific And Industrial Research Method for inducing improved seed germination in Podophyllum hexandrum Royle
CN101189943A (en) * 2006-11-18 2008-06-04 怀化医学高等专科学校 Seed propagating fast breeding method for paris polyphylla
CN102835207A (en) * 2012-09-19 2012-12-26 贵州三元太宝实业股份有限公司 Method for reducing sleeping cycle of Paris polyphylla seeds
CN104541655A (en) * 2013-10-15 2015-04-29 云南白药集团中药材优质种源繁育有限责任公司 Method for breaking paris polyphylla seed dormancy
CN110089378A (en) * 2019-05-24 2019-08-06 成都市农林科学院 A kind of efficient Paris polyphylla seed seedling-raising method

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