CN111000955A

CN111000955A - Firming antibacterial gel and preparation method thereof

Info

Publication number: CN111000955A
Application number: CN201911398276.9A
Authority: CN
Inventors: 杨国军; 蒋清昀
Original assignee: Chongqing Gongmiao Biotechnology Co ltd
Current assignee: Chongqing Gongmiao Biotechnology Co ltd
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2020-04-14

Abstract

The invention discloses a compact antibacterial gel, which comprises the following components: the feed comprises traditional Chinese medicine extracting solution, tocopherol, lactobacillus, pear juice fermentation product filtrate, carbopol, propylene glycol, glycerol, menthol and borneol, wherein the traditional Chinese medicine extracting solution mixed solution is prepared from the following traditional Chinese medicines: fructus Cnidii extractive solution, stigma croci Sativi extractive solution, radix Arnebiae extractive solution, radix Stemonae extractive solution, radix Angelicae sinensis extractive solution, radix Sophorae Flavescentis extractive solution, and folium Canarii albi extractive solution. By adopting the technical scheme, the common cnidium fruit extracting solution, the saffron extracting solution, the lithospermum extracting solution, the radix stemonae extracting solution, the Chinese angelica extracting solution, the radix sophorae flavescentis extracting solution and the olive leaf extracting solution are mutually cooperated, so that the growth and the reproduction of bacteria can be effectively inhibited, the vaginal mucosa and cervical injury can be repaired, the vagina can be promoted to recover elasticity and tightness, the ovary can be protected, the immunity of the organism can be improved, and the female reproductive health can be promoted.

Description

Firming antibacterial gel and preparation method thereof

Technical Field

The invention relates to the technical field of gynecological medicines, in particular to a firming and antibacterial gel and a preparation method thereof.

Background

The vagina is a very important pipeline for connecting the female internal and external genitalia, the upper end of the vagina is connected with the uterus, the lower end of the vagina is a vaginal orifice, the vagina is an open cavity, the vagina is an important micro-ecological area of the female, and the micro-environment is complex and unstable. The cuticle of vagina is thin, which can protect hair, and there are some sweat glands and special sebaceous glands to maintain the pH value and lubricity of vagina skin. With the increase of the age of women, people living or breeding and the like destroy the balanced environment of the vagina, so that bacteria are bred, inflammation and vaginal muscle relaxation are caused, and the physical and mental health of women is influenced.

Disclosure of Invention

The invention provides a compact antibacterial gel and a preparation method thereof.

In order to solve the technical problems, the technical scheme of the invention is that the compact antibacterial gel comprises the following components: the feed comprises traditional Chinese medicine extracting solution, tocopherol, lactobacillus, pear juice fermentation product filtrate, carbopol, propylene glycol, glycerol, menthol and borneol, wherein the traditional Chinese medicine extracting solution mixed solution is prepared from the following traditional Chinese medicines: fructus Cnidii extractive solution, stigma croci Sativi extractive solution, radix Arnebiae extractive solution, radix Stemonae extractive solution, radix Angelicae sinensis extractive solution, radix Sophorae Flavescentis extractive solution, and folium Canarii albi extractive solution.

Further, the antibacterial gel comprises the following components in parts by weight: 40-80 parts of traditional Chinese medicine extracting solution, 5-10 parts of tocopherol, 20-30 parts of lactobacillus, 8-16 parts of pear juice fermentation product filtrate, 8-20 parts of carbopol, 5-10 parts of propylene glycol, 5-10 parts of glycerol, 40-60 parts of glycerol and 2-8 parts of menthol.

Further, the traditional Chinese medicine extracting solution is prepared from the following traditional Chinese medicines in parts by weight: 8-12 parts of fructus cnidii extract, 2-4 parts of saffron extract, 6-9 parts of lithospermum extract, 1-3 parts of radix stemonae extract, 2-4 parts of angelica extract, 1-3 parts of radix sophorae flavescentis extract and 5-7 parts of olive leaf extract.

A preparation method of a compact antibacterial gel comprises the following steps:

(1) selecting fructus cnidii, removing impurities, drying in the sun, crushing, sieving with a sieve of 80-100 meshes to obtain fructus cnidii powder, soaking in a 200-time volume of 1% NaOH solution at 22-25 ℃ for 50-70 min, carrying out ultrasonic extraction for 8-12 min, adding 10% HCL into an extracting solution, acidifying, centrifuging, taking a precipitate, adding 5-10 times methanol into the precipitate for dissolving to obtain a dissolving solution, and filtering the dissolving solution by using a 0.45-micrometer microporous membrane to obtain the fructus cnidii extracting solution.

(2) Selecting saffron, removing impurities, drying in an oven at 40-50 ℃ for 20-30 min, crushing by a high-speed crusher, sieving by a 40-60-mesh sieve to obtain saffron powder, adding ethanol into the saffron powder according to a material-liquid ratio of 1: 30-1: 50, wherein the concentration of the ethanol is 40-70%, soaking and oscillating for 20-40 min at the temperature of 20-30 ℃, and performing ultrasonic extraction for 20-50 min at the ultrasonic temperature of 40-60 ℃ to obtain a saffron extracting solution.

(3) Selecting lithospermum, adding 75-95% ethanol, soaking for 30-50 min, wherein the material-liquid ratio is 1: 10-1: 13, and using an ultrasonic microwave synergistic extraction instrument, wherein the ultrasonic power is 50W, the microwave power is 70W, the extraction temperature is 20-60 ℃, and the extraction time is 5-10 min, so as to obtain lithospermum extract.

(4) Selecting radix stemonae, washing with clear water, drying, pulverizing in a plant pulverizer, sieving with a 20-30 mesh sieve to obtain radix stemonae powder, adding ethanol solution, heating and refluxing, concentrating, adjusting pH, filtering to obtain extractive solution, separating and purifying the extractive solution with macroporous adsorbent resin, collecting ethanol eluate, and concentrating under reduced pressure to obtain radix stemonae extractive solution.

(5) Selecting angelica, spraying clear water, cleaning, fishing out, drying, crushing, sieving with a 20-60-mesh sieve to obtain angelica powder, respectively adding water, heating, refluxing and extracting for 3 times, wherein the water adding amount for 3 times of extraction is 6 times, 8 times and 10 times respectively, extracting for 1-1.5 h each time, filtering, respectively collecting extracting solutions for each time, and mixing the extracting solutions filtered for three times to obtain the angelica extracting solution.

(6) Selecting radix sophorae flavescentis, fishing out after spray cleaning, drying, crushing, sieving with a 20-60-mesh sieve to obtain radix sophorae flavescentis powder, soaking with 2-4 times of 60-80% ethanol in volume for 20-30 hours, and percolating at the flow rate of 5ml/min to obtain the radix sophorae flavescentis extract.

(7) Selecting olive leaves, spraying, cleaning, drying in a vacuum freeze dryer, pulverizing in a high-speed pulverizer, sieving with a 20-60 mesh sieve to obtain olive leaf powder, adding ethanol, performing ultrasonic-assisted extraction for 20-40 min, and centrifuging to obtain supernatant to obtain olive leaf extract.

(8) And (3) adding the extracting solution obtained in the step (1) to the extracting solution obtained in the step (7) into the pear juice fermentation product filtrate, mixing the filtrate in proportion to obtain a mixed solution, filtering the mixed solution, combining the filtered solution, and concentrating the combined filtrate until the relative density is 1.1-1.20 to obtain the traditional Chinese medicine extracting solution extract with the alcohol content of 60 percent.

(9) Mixing tocopherol, propylene glycol, glycerol, menthol and borneol, and adding 95% ethanol for dissolving to obtain solution A.

(10) And (3) fully and uniformly mixing the lactobacillus and the carbopol, and adding water to fully swell to obtain a solution B.

(11) And (3) fully mixing the traditional Chinese medicine extract obtained in the step (8), the solution A obtained in the step (9) and the solution B obtained in the step (10), and extinguishing to obtain the firming and bacteriostasis gel.

Further, the device for drying the stemona root is an electric heating constant temperature blast drying box in the step (4).

Further, in the step (4), the pH is adjusted by using an aqueous sodium hydroxide solution.

Further, in the step (4), the specific surface area of the macroporous adsorption resin is 1300.

Further, in the step (10), the added water is distilled water.

By adopting the technical scheme, the common cnidium fruit extracting solution, the saffron extracting solution, the lithospermum extracting solution, the radix stemonae extracting solution, the Chinese angelica extracting solution, the radix sophorae flavescentis extracting solution and the olive leaf extracting solution are mutually cooperated, so that the growth and the reproduction of bacteria can be effectively inhibited, the vaginal mucosa and cervical injury can be repaired, the vagina can be promoted to recover elasticity and tightness, the ovary can be protected, the immunity of the organism can be improved, and the female reproductive health can be promoted.

Detailed Description

The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

The mixed liquid of the traditional Chinese medicine extract comprises a plurality of components, wherein the saffron extract is a product extracted from the upper part and stigma of crocus sativus style of iridaceae, the main components of the saffron extract comprise picrorhizin, carotene compounds, carthamal, eucalyptol, pinene and other substances, and also comprise a small amount of isorhamnetin, kaempferide, vitamin B1, vitamin B2 and the like. The saffron extract has obvious effects of promoting blood circulation to remove blood stasis, resisting bacteria and diminishing inflammation and the like, so that the body endurance is enhanced, the content of immune cells is increased, the immunity of a human body is improved, and the effects of regulating qi and blood circulation of the body and balancing yin and yang of the human body are achieved.

Zi Cao is sweet and cold in nature and can cool blood and activate blood. Alkannin has anticancer, antiinflammatory, and antibacterial effects. It can be used for treating acute and chronic hepatitis, and liver cirrhosis (ascites). The family Dermatology is used for treating flat warts, psoriasis, for treating burns and for promoting wound healing by topical application. The eye drop can be used for treating herpes simplex keratitis, and has therapeutic effect on epithelial dendritic keratitis and superficial parenchymal layer dendritic keratitis. The oil can be used for treating infantile dermatitis, eczema, vaginitis, and cervicitis. The lithospermum mainly contains naphthoquinone pigment substances, is difficult to dissolve in water, and is easy to dissolve in organic solvents such as ethanol, petroleum ether, chloroform, ethyl acetate and the like. In consideration of production cost and practical production conditions, ethanol should be selected as a solvent. The ultrasonic waves and the microblogs have thermal effect, mechanical effect and cavitation effect, so that the plant cells are easy to crack, the effective components in the cells are easy to extract at low temperature and high speed.

TABLE 1 Quadrature experiment of extraction of Lithospermum erythrorhizon

Stemona is a very common plant, but it has unusual effects and effects, the in vitro experiment of Stemona shows, Stemona has therapeutic effects on head louse, body louse, pinworm disease, pudendum itch, while extracting Stemona, extract of Stemona, regarding the choice of extractant kind, choose 5g of material 5, it is ① 75% acetone (V/V), ② distilled water, ③ 75% ethanol (V/V), ④ 75% diethyl ether/ethanol (V/V), ⑤ 75% methanol respectively under the same condition, cold soak for 12h, dissolve to 100ml definitely, filter, absorb 1ml filtrate, determine A_760nmThe value is obtained.

TABLE 2 Effect of solvent species on tannin extraction in Baizhou

Kind of solvent	Absorbance value
		75% acetone	0.42
Distilled water	0.36
		75% ethanol	0.40
75% Ether/ethanol	0.37
		75% methanol	0.41

As can be seen from table 2, under the same conditions, the extraction effects of 75% acetone, 75% ethanol and 75% methanol are similar, but in consideration of the safety and cost of the reagents, ethanol is selected as the extractant in the experiment.

TABLE 3 Stemona root Total alkaloid extraction Process factor horizon

The angelica has antibacterial effects on dysentery in vitro, typhoid fever, paratyphoid fever, escherichia coli, diphtheria bacillus, vibrio cholerae, α, β hemolytic streptococcus and the like, can accelerate the healing of rabbit ear wound by external application, enables local congestion, leukocyte and fiber infiltration and regeneration of new epithelium, and has the effects of stopping bleeding and strengthening peripheral circulation on local tissues.

TABLE 4 Chinese angelica extraction process factor horizon

Olive leaf extract and its oleuropein component are the best known of their blood pressure lowering effect, but recent studies have revealed that their health benefits also include anti-inflammatory and antioxidant capabilities, and modern medicine uses olive leaf extract essence to help fight against atherosclerosis, diabetes, cancer, neurodegenerative diseases and even arthritis. The present application uses the antibacterial properties of olive leaf, olive leaf extract oleuropein has a resistance against microorganisms, because it contains calcium enoate compound, which has strong antibacterial and antiviral effects and can prevent further disease transmission by bacteria and viruses. Processing by vacuum freeze drying technology to freeze the medicinal material at low temperature, directly converting frozen solid water into water vapor to sublimate without liquid state under vacuum state, and obtaining freeze-dried medicinal material when the water content of the medicinal material is below 5%. The freeze-dried medicinal material has low loss of low-temperature volatile components, retains the original bioactive components of the medicinal material, exerts the value of the Chinese medicinal material to the maximum extent, restores the active components and the drug effect in a fresh state, and simultaneously ensures that the active components are more easily separated out by a wall breaking process.

Example one

The firming antibacterial gel comprises the following components in parts by weight: 40 parts of traditional Chinese medicine extracting solution, 5 parts of tocopherol, 20 parts of lactobacillus, 8 parts of pear juice fermentation product filtrate, 8 parts of carbopol, 5 parts of propylene glycol, 5 parts of glycerol, 40 parts of glycerol and 2 parts of menthol.

The traditional Chinese medicine extracting solution is prepared from the following traditional Chinese medicines in parts by weight: 8 parts of fructus cnidii extract, 2 parts of saffron extract, 6 parts of lithospermum extract, 1 part of radix stemonae extract, 2 parts of angelica extract, 1 part of radix sophorae flavescentis extract and 5 parts of olive leaf extract.

(1) selecting fructus Cnidii, removing impurities, sun drying, pulverizing, sieving with 80 mesh sieve to obtain fructus Cnidii powder, soaking with 200 times volume of 1% NaOH solution at 22 deg.C for 50min, ultrasonic extracting for 8min, adding 10% HCl into the extractive solution, acidifying, centrifuging, collecting precipitate, adding 5 times methanol into the precipitate for dissolving to obtain solution, and filtering with 0.45 μm microporous membrane to obtain fructus Cnidii extractive solution.

(2) Selecting saffron crocus, removing impurities, drying in a drying oven at 40 ℃ for 20min, crushing by using a high-speed crusher, sieving by using a 40-mesh sieve to obtain saffron crocus powder, adding ethanol into the saffron crocus powder at a material-liquid ratio of 1:30 and an ethanol concentration of 40%, soaking and oscillating at a temperature of 20 ℃ for 20min, and performing ultrasonic extraction for 20min at an ultrasonic temperature of 40 ℃ to obtain a saffron crocus extracting solution.

(3) Selecting radix Arnebiae, soaking in 75% ethanol for 30min at a material-to-liquid ratio of 1:10, and extracting with ultrasonic microwave synergistic extractor at 20 deg.C for 5min under ultrasonic power of 50W and microwave power of 70W to obtain radix Arnebiae extractive solution.

(4) Selecting radix stemonae, washing with clear water, drying, crushing in a plant crusher, sieving with a 20-mesh sieve to obtain radix stemonae powder, adding an ethanol solution, heating and refluxing, concentrating, adjusting the pH value with a sodium hydroxide aqueous solution, filtering to obtain an extract, separating and purifying the extract by using macroporous adsorption resin, wherein the specific surface area of the macroporous adsorption resin is 1300, collecting ethanol eluate, and concentrating under reduced pressure to obtain the radix stemonae extract, wherein the device for drying radix stemonae is an electric heating constant-temperature blast drying box.

(5) Selecting angelica, spraying clear water for cleaning, fishing out, drying, crushing, sieving with a 20-mesh sieve, obtaining angelica powder, respectively adding water, heating, refluxing and extracting for 3 times, wherein the water adding amount for 3 times of extraction is 6 times, 8 times and 10 times respectively, extracting for 1 hour each time, respectively collecting extracting solutions for each time after filtering, and mixing the extracting solutions obtained after three times of filtering to obtain the angelica extracting solution.

(6) Selecting radix Sophorae Flavescentis, cleaning by spraying, taking out, oven drying, pulverizing, sieving with 20 mesh sieve to obtain radix Sophorae Flavescentis powder, soaking in 2 times volume of 60% ethanol for 20 hr, and percolating at flow rate of 5ml/min to obtain radix Sophorae Flavescentis extractive solution.

(7) Selecting olive leaves, spray-cleaning, drying in a vacuum freeze dryer, pulverizing in a high-speed pulverizer, sieving with 20 mesh sieve to obtain olive leaf powder, adding ethanol, ultrasonic extracting for 20min, and centrifuging to obtain supernatant to obtain olive leaf extractive solution.

(8) And (3) adding the extracting solution obtained in the step (1) to the extracting solution obtained in the step (7) into the pear juice fermentation product filtrate, mixing the filtrate according to a proportion to obtain a mixed solution, filtering the mixed solution, combining the filtered solution, and concentrating the combined filtrate until the relative density is 1.1 to obtain the traditional Chinese medicine extracting solution extract with the alcohol content of 60 percent.

(10) Mixing lactobacillus and carbopol, adding distilled water, and swelling to obtain solution B.

Example two

The firming antibacterial gel comprises the following components in parts by weight: 60 parts of traditional Chinese medicine extracting solution, 8 parts of tocopherol, 25 parts of lactobacillus, 12 parts of pear juice fermentation product filtrate, 16 parts of carbopol, 8 parts of propylene glycol, 8 parts of glycerol, 50 parts of glycerol and 5 parts of menthol.

The traditional Chinese medicine extracting solution is prepared from the following traditional Chinese medicines in parts by weight: 10 parts of fructus cnidii extract, 3 parts of saffron extract, 7 parts of lithospermum extract, 2 parts of radix stemonae extract, 3 parts of angelica extract, 2 parts of radix sophorae flavescentis extract and 6 parts of olive leaf extract.

(1) selecting fructus Cnidii, removing impurities, sun drying, pulverizing, sieving with 90 mesh sieve to obtain fructus Cnidii powder, soaking with 200 times volume of 1% NaOH solution at 22 deg.C for 60min, ultrasonic extracting for 10min, adding 10% HCl into the extractive solution, acidifying, centrifuging, collecting precipitate, adding 8 times methanol into the precipitate for dissolving to obtain solution, and filtering with 0.45 μm microporous membrane to obtain fructus Cnidii extractive solution.

(2) Selecting saffron crocus, removing impurities, drying in a 45 ℃ oven for 25min, crushing by a high-speed crusher, sieving by a 50-mesh sieve to obtain saffron crocus powder, adding ethanol into the saffron crocus powder at a material-liquid ratio of 1:40 and an ethanol concentration of 50%, soaking and oscillating at a temperature of 25 ℃ for 30min, and performing ultrasonic extraction for 30min at an ultrasonic temperature of 50 ℃ to obtain a saffron crocus extracting solution.

(3) Selecting radix Arnebiae, soaking in 85% ethanol at a material-to-liquid ratio of 1:12 for 40min, and extracting with ultrasonic microwave synergistic extractor at 40 deg.C for 8min under ultrasonic power of 50W and microwave power of 70W to obtain radix Arnebiae extractive solution.

(4) Selecting radix stemonae, washing with clear water, drying, putting the radix stemonae into a plant grinder for grinding, sieving with a 20-30-mesh sieve to obtain radix stemonae powder, adding an ethanol solution, heating and refluxing, concentrating, adjusting the pH value by using a sodium hydroxide aqueous solution, filtering to obtain an extracting solution, separating and purifying the extracting solution by using macroporous adsorption resin, wherein the specific surface area of the macroporous adsorption resin is 1300, collecting ethanol eluent, and concentrating under reduced pressure to obtain the radix stemonae extracting solution, wherein the device for drying the radix stemonae is an electric heating constant-temperature blast drying box.

(5) Selecting angelica, spraying clear water for cleaning, fishing out, drying, crushing, sieving with a 40-mesh sieve, obtaining angelica powder, respectively adding water, heating, refluxing and extracting for 3 times, wherein the water adding amount for 3 times of extraction is respectively 6 times, 8 times and 10 times, the extraction time is 1.2h each time, respectively collecting the extracting solution for each time after filtering, and mixing the extracting solutions obtained after three times of filtering to obtain the angelica extracting solution.

(6) Selecting radix Sophorae Flavescentis, cleaning by spraying, taking out, oven drying, pulverizing, sieving with 40 mesh sieve to obtain radix Sophorae Flavescentis powder, soaking in 3 times volume of 70% ethanol for 25 hr, and percolating at flow rate of 5ml/min to obtain radix Sophorae Flavescentis extractive solution.

(7) Selecting olive leaves, spray-cleaning, drying in a vacuum freeze dryer, pulverizing in a high-speed pulverizer, sieving with 40 mesh sieve to obtain olive leaf powder, adding ethanol, ultrasonic extracting for 30min, centrifuging, and collecting supernatant to obtain olive leaf extractive solution.

EXAMPLE III

The firming antibacterial gel comprises the following components in parts by weight: 80 parts of traditional Chinese medicine extracting solution, 10 parts of tocopherol, 30 parts of lactobacillus, 16 parts of pear juice fermentation product filtrate, 20 parts of carbopol, 10 parts of propylene glycol, 10 parts of glycerol, 60 parts of glycerol and 8 parts of menthol.

The traditional Chinese medicine extracting solution is prepared from the following traditional Chinese medicines in parts by weight: 12 parts of fructus cnidii extract, 4 parts of saffron extract, 9 parts of lithospermum extract, 3 parts of radix stemonae extract, 4 parts of angelica extract, 3 parts of radix sophorae flavescentis extract and 7 parts of olive leaf extract.

(1) selecting fructus Cnidii, removing impurities, sun drying, pulverizing, sieving with 100 mesh sieve to obtain fructus Cnidii powder, soaking with 200 times volume of 1% NaOH solution at 25 deg.C for 70min, ultrasonic extracting for 12min, adding 10% HCl into the extractive solution, acidifying, centrifuging, collecting precipitate, adding 10 times volume of methanol into the precipitate, dissolving to obtain solution, and filtering with 0.45 μm microporous membrane to obtain fructus Cnidii extractive solution.

(2) Selecting saffron crocus, removing impurities, drying in a 50 ℃ oven for 30min, crushing by a high-speed crusher, sieving by a 60-mesh sieve to obtain saffron crocus powder, adding ethanol into the saffron crocus powder at a material-liquid ratio of 1:50 and an ethanol concentration of 70%, soaking and shaking at a temperature of 30 ℃ for 40min, and performing ultrasonic extraction for 50min at an ultrasonic temperature of 60 ℃ to obtain a saffron crocus extracting solution.

(3) Selecting radix Arnebiae, soaking in 95% ethanol for 50min at a material-to-liquid ratio of 1:13, and extracting with ultrasonic microwave synergistic extractor at 60 deg.C for 10min under ultrasonic power of 50W and microwave power of 70W to obtain radix Arnebiae extractive solution.

(4) Selecting radix stemonae, washing with clear water, drying, crushing in a plant crusher, sieving with a 30-mesh sieve to obtain radix stemonae powder, adding an ethanol solution, heating and refluxing, concentrating, adjusting the pH value with a sodium hydroxide aqueous solution, filtering to obtain an extract, separating and purifying the extract by using macroporous adsorption resin, wherein the specific surface area of the macroporous adsorption resin is 1300, collecting ethanol eluate, and concentrating under reduced pressure to obtain the radix stemonae extract, wherein the device for drying radix stemonae is an electric heating constant-temperature blast drying box.

(5) Selecting angelica, spraying clear water for cleaning, fishing out, drying, crushing, sieving with a 60-mesh sieve, obtaining angelica powder, respectively adding water, heating, refluxing and extracting for 3 times, wherein the water adding amount for 3 times of extraction is respectively 6 times, 8 times and 10 times, the extraction time is 1.5h each time, respectively collecting the extracting solution for each time after filtering, and mixing the extracting solutions obtained after three times of filtering to obtain the angelica extracting solution.

(6) Selecting radix Sophorae Flavescentis, cleaning by spraying, taking out, oven drying, pulverizing, sieving with 60 mesh sieve to obtain radix Sophorae Flavescentis powder, soaking in 4 times volume of 80% ethanol for 30 hr, and percolating at flow rate of 5ml/min to obtain radix Sophorae Flavescentis extractive solution.

(7) Selecting olive leaves, spray-cleaning, drying in a vacuum freeze dryer, pulverizing in a high-speed pulverizer, sieving with 60 mesh sieve to obtain olive leaf powder, adding ethanol, ultrasonic extracting for 40min, centrifuging, and collecting supernatant to obtain olive leaf extractive solution.

The second embodiment is the best embodiment.

The invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.

Claims

1. The firming antibacterial gel is characterized by comprising the following components: the feed comprises traditional Chinese medicine extracting solution, tocopherol, lactobacillus, pear juice fermentation product filtrate, carbopol, propylene glycol, glycerol, menthol and borneol, wherein the traditional Chinese medicine extracting solution mixed solution is prepared from the following traditional Chinese medicines: fructus Cnidii extractive solution, stigma croci Sativi extractive solution, radix Arnebiae extractive solution, radix Stemonae extractive solution, radix Angelicae sinensis extractive solution, radix Sophorae Flavescentis extractive solution, and folium Canarii albi extractive solution.

2. The tightening bacteriostatic gel according to claim 1, which comprises the following components in parts by weight: 40-80 parts of traditional Chinese medicine extracting solution, 5-10 parts of tocopherol, 20-30 parts of lactobacillus, 8-16 parts of pear juice fermentation product filtrate, 8-20 parts of carbopol, 5-10 parts of propylene glycol, 5-10 parts of glycerol, 40-60 parts of glycerol and 2-8 parts of menthol.

3. The tightening antibacterial gel as claimed in claim 1, wherein the traditional Chinese medicine extract is prepared from the following traditional Chinese medicines in parts by weight: 8-12 parts of fructus cnidii extract, 2-4 parts of saffron extract, 6-9 parts of lithospermum extract, 1-3 parts of radix stemonae extract, 2-4 parts of angelica extract, 1-3 parts of radix sophorae flavescentis extract and 5-7 parts of olive leaf extract.

4. The method for preparing the compact bacteriostatic gel according to any one of claims 1 to 3, characterized by comprising the following steps:

(1) selecting fructus cnidii, removing impurities, drying in the sun, crushing, sieving with a sieve of 80-100 meshes to obtain fructus cnidii powder, soaking in a 200-time volume of 1% NaOH solution at 22-25 ℃ for 50-70 min, carrying out ultrasonic extraction for 8-12 min, adding 10% HCL into an extracting solution, acidifying, centrifuging, taking a precipitate, adding 5-10 times of methanol into the precipitate for dissolving to obtain a dissolving solution, and filtering the dissolving solution by using a 0.45-micrometer microporous membrane to obtain a fructus cnidii extracting solution;

(2) selecting saffron crocus, removing impurities, drying in a drying oven at 40-50 ℃ for 20-30 min, crushing by a high-speed crusher, sieving by a 40-60-mesh sieve to obtain saffron crocus powder, adding ethanol into the saffron crocus powder according to a material-liquid ratio of 1: 30-1: 50, soaking and vibrating at the temperature of 20-30 ℃ for 20-40 min, and performing ultrasonic extraction for 20-50 min at the ultrasonic temperature of 40-60 ℃ to obtain a saffron extracting solution;

(3) selecting lithospermum, adding 75-95% ethanol to soak for 30-50 min, wherein the material-liquid ratio is 1: 10-1: 13, and using an ultrasonic microwave synergistic extraction instrument, wherein the ultrasonic power is 50W, the microwave power is 70W, the extraction temperature is 20-60 ℃, and the extraction time is 5-10 min, so as to obtain lithospermum extract;

(4) selecting radix stemonae, washing with clear water, drying, pulverizing in a plant pulverizer, sieving with a 20-30 mesh sieve to obtain radix stemonae powder, adding ethanol solution, heating and refluxing, concentrating, adjusting pH, filtering to obtain extractive solution, separating and purifying the extractive solution with macroporous adsorbent resin, collecting ethanol eluate, and concentrating under reduced pressure to obtain radix stemonae extractive solution;

(5) selecting angelica, spraying clear water, cleaning, fishing out, drying, crushing, sieving with a 20-60-mesh sieve to obtain angelica powder, respectively adding water, heating, refluxing and extracting for 3 times, wherein the water adding amount for 3 times of extraction is 6 times, 8 times and 10 times respectively, each time of extraction is 1-1.5 hours, respectively collecting extracting solutions for each time after filtering, and mixing the extracting solutions after three times of filtering to obtain the angelica extracting solution;

(6) selecting radix sophorae flavescentis, fishing out after spraying and cleaning, drying, crushing, sieving with a 20-60-mesh sieve to obtain radix sophorae flavescentis powder, soaking with 2-4 times of 60-80% ethanol in volume for 20-30 hours, and percolating at the flow rate of 5ml/min to obtain radix sophorae flavescentis extract;

(7) selecting olive leaves, spraying and cleaning, drying in a vacuum freeze dryer, crushing in a high-speed crusher, sieving with a 20-60-mesh sieve to obtain olive leaf powder, adding ethanol for ultrasonic assisted extraction for 20-40 min, and centrifuging to obtain supernatant to obtain olive leaf extract;

(8) adding the extracting solution obtained in the step (1) to the extracting solution obtained in the step (7) into the pear juice fermentation product filtrate, mixing according to a proportion to obtain a mixed solution, filtering, combining, and concentrating the combined filtrate to a relative density of 1.1-1.20 to obtain a traditional Chinese medicine extracting solution extract with an alcohol content of 60%;

(9) mixing tocopherol, propylene glycol, glycerol, menthol and borneol, and adding 95% ethanol for dissolving to obtain a solution A;

(10) sufficiently and uniformly mixing lactobacillus and carbopol, and adding water to sufficiently swell to obtain a solution B;

5. The preparation method of the compact antibacterial gel as claimed in claim 4, wherein in the step (4), the device for drying radix stemonae is an electric heating constant temperature air blast drying oven.

6. The method for preparing the compact bacteriostatic gel according to claim 4, wherein in the step (4), the pH value is adjusted by using an aqueous solution of sodium hydroxide.

7. The method for preparing the compact bacteriostatic gel according to claim 4, wherein in the step (4), the specific surface area of the macroporous adsorbent resin is 1300.

8. The method for preparing the compact bacteriostatic gel according to claim 4, wherein in the step (10), the added water is distilled water.