CN102119964A

CN102119964A - Extract of preventing and treating coronary heart disease and stenocardia and preparation method and application thereof

Info

Publication number: CN102119964A
Application number: CN2010100313154A
Authority: CN
Inventors: 刘岩; 丛德刚; 刘顺航; 徐波; 戚可人; 陈红; 赵国辉
Original assignee: TIANJIN TASLY MODERN CHINESE MEDICINE RESOURCE CO Ltd
Current assignee: TIANJIN TASLY MODERN CHINESE MEDICINE RESOURCE CO Ltd
Priority date: 2010-01-07
Filing date: 2010-01-07
Publication date: 2011-07-13
Anticipated expiration: 2030-01-07
Also published as: CN102119964B

Abstract

The invention relates to an extract of preventing and treating coronary heart disease and stenocardia and a preparation method and application thereof. The preparation method comprises the following steps of: 1, extracting medicinal materials such as red-rooted salvia root and pseudo-ginseng to obtain an extraction solution; 2, separating the extraction solution by using macroporous resin to obtain an extract; and 3, taking the extract and borneol together serving as medicinal active ingredients to prepare a medicinal preparation.

Description

The extract of a kind of prevention and treatment angina pectoris, their Preparation method and use

Technical field:

The present invention relates to the process for purification of the extracting method of cardiovascular drugs compound red sage root preparation, particularly extract.

Background technology:

Compound red sage root preparation is made up of Radix Salviae Miltiorrhizae, Radix Notoginseng, Borneolum Syntheticum etc., the effect that blood circulation promoting and blood stasis dispelling, regulating QI to relieve pain are arranged, compound red sage root preparation prolonged application in the clinical disease that has a microcirculation disturbance, evident in efficacy in the treatment of Medicine Systems disease such as cardiovascular and cerebrovascular disease, respiratory system disease, digestive system disease, renal system disease etc.Therefore purposes more and more widely.In recent years, except that the Medicine Systems disease, also extensively adopt at department of dermatologry.

The main aspect of the therapeutical effect of compound red sage root preparation is: coronary heart disease, angina pectoris and microcirculation patients with coronary heart disease nail fold microcirculation show tangible circulatory disturbance.Systemic microcirculation can think that the nail fold microcirculation is whole circulation window by the performance of nail fold microcirculation.

Shen shapes etc. use compound red sage root preparation to treat to 62 routine patients with coronary heart disease.These patient's nail fold pipe loop decreased number, pipe loop intersection deformity increases, and pipe loop diameter reduces, and blood flow rate slows down, the minimizing of line grain stream, erythrocyte gathers obviously.The microcirculatory this abnormal change of nail fold, proved that patients with coronary heart disease exists cardiac microcirculation disturbance, after above patient treated through compound red sage root preparation, the microcirculatory every index of nail fold all had clear improvement and takes a turn for the better, the total effective rate of clinical treatment reaches 91%, and ECG improvement rate is 72%.

Li Feng etc. are divided into routine of western medicine treatment group (oral sorbitrate, quiet polarized solution add Energy mixture) and composite salvia dropping pill for curing group (except that conventional western medicine with 90 routine angina pectoris patients, add the red ball of compound Salviae Miltiorrhizae, each 10, every day 3 times) totally 20 day, the angina of drop pill group treatment as a result total effective rate 92.2% is significantly higher than routine of western medicine treatment group 68.0% (P＜0.05).Drop pill group ECG effective percentage 74.4% also is significantly higher than routine of western medicine group 48.0% (P＜0.05), and the drop pill group all significantly improves (P＜0.01) than the Western medicine group to the remission rate of symptoms such as patient's simultaneous phenomenon such as uncomfortable in chest, cardiopalmus, limb fiber crops, insomnia.

Red sage formulation mainly contains in the pharmacological action aspect the treatment cardiovascular disease:

(1) dilating coronary blood vessel improves coronary blood flow

(2) microcirculation improvement obstacle

(3) improve hemorheological property

(4) anticoagulant

(5) resisting oxygen lack

(6) anti peroxidation of lipid and elimination free radical

The preparation method of existing compound red sage root preparation is a lot, carries alcohol extraction, water extract-alcohol precipitation, alcohol extracting-water precipitating etc. as water.

Extract is not carried out effective ingredient monitoring and purified step in the prior art, to such an extent as to existing compound red sage root preparation lacks means on quality control, the content of active ingredient still remains to be improved.

Summary of the invention:

The invention provides a kind of extracting method of compound red sage root preparation and the compound red sage root preparation for preparing by this method.

Compound red sage root preparation of the present invention is prepared from by following raw materials by weight percent medicine,

Radix Salviae Miltiorrhizae 20%-97%, Radix Notoginseng 2.0%-79%, Borneolum Syntheticum 0.2%-3.0%.

Preferred Radix Salviae Miltiorrhizae 63.0%-94% Radix Notoginseng 4.0%-35.0% Borneolum Syntheticum 0.5%-2.0%.

Preferred Radix Salviae Miltiorrhizae 75.2%-90% Radix Notoginseng 9%-23.5% Borneolum Syntheticum 0.5%-1.3%.

Most preferred Radix Salviae Miltiorrhizae 82.87% Radix Notoginseng 16.21% Borneolum Syntheticum 0.92%.

Above pharmaceutical formulation is that the blood circulation promoting and blood stasis dispelling according to the traditional Chinese medical science, the effect of regulating QI to relieve pain are determined, situation according to patient, wherein Borneolum Syntheticum can substitute with other drug or pharmaceutical preparations, as volatile oil of Lignum Dalbergiae Odoriferae, Styrax, Rhizoma Chuanxiong extract, or wherein any compositions, or other invigorating blood circulation (breaking) are become silted up, the regulating QI to relieve pain medicine.Do not affect the treatment, therefore, the increase of these prescriptions all belongs to scope of the present invention.

Compound red sage root preparation of the present invention, preparation formulation is selected from: the quick releasing formulation of tablet, capsule, drop pill, pill and these dosage forms thereof or durative action preparation, or powder spray, aerosol, gel, injection, lyophilizing divide injection, Orally-disintegrating tablet etc.Preferred dosage form is a drop pill.

According to the galenic pharmacy needs, can add during preparation pharmaceutic adjuvant as: starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, methyl starch sodium, cross-linking sodium carboxymethyl cellulose, crospolyvinylpyrrolidone etc. are separately or the various adjuvants that are mixed and made into.

The present invention also comprises with the application of composite salvia dropping pill of the present invention in the medicine of cardiovascular disease such as a kind of resisting coronary heart disease of preparation, angina pectoris.

The preparation of compound red sage root preparation of the present invention is earlier crude drug to be prepared into active constituents of medicine through following steps, further is prepared into pharmaceutical preparation again.

Preparation method of the present invention, method is as follows:

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng extract, and get extracting solution;

Step 2, extract refining obtains extract;

Step 3 together as active constituents of medicine, is prepared into pharmaceutical preparation with the galenic pharmacy routine techniques with extract and Borneolum Syntheticum.

Preparation method of the present invention, preferable methods is as follows:

In the step 1, described Radix Salviae Miltiorrhizae, pseudo-ginseng are the products as the raw material of medication preparation of the forms such as medical material, decoction pieces or powder of Radix Salviae Miltiorrhizae, Radix Notoginseng, can buy from the market, also can process according to pharmacopeia, the described water that is extracted as is carried, described extraction can adopt Radix Salviae Miltiorrhizae, panax mixed to extract, or Radix Salviae Miltiorrhizae, Radix Notoginseng extract separately respectively, extracts the back and mixes.

In the step 2, the described refining macroporous resin partition method that is, described macroporous resin partition method: be extracting solution to be separated through the macroporous resin partition method purify;

In the step 3, the extract of step 2 and Borneolum Syntheticum are prepared into dropping pill formulation together as active constituents of medicine with the galenic pharmacy routine techniques.

Preparation method of the present invention, preferred method is as follows:

In the step 1, described extracting mode is selected from decocting method, warm macerating method, enzyme process assisted extraction, continuous dynamic countercurrent extraction, reflux, extract,, percolation extraction, microwave extraction or hyperpressure extraction etc.

In the step 2, extracting solution concentrates or does not concentrate and uses macroporous resin to separate remove impurity, and eluent concentrates or be dry, the macroporous resin of the preferred nonpolar or low pole of macroporous resin;

Method for concentration is that normal pressure concentrates, concentrating under reduced pressure etc.

Drying means is constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying, far-infrared ray drying etc.

In the step 3, described dropping pill formulation, during preparation there be adoptable adjuvant: water-soluble base has Polyethylene Glycol (PEG), polyoxyethylene monostearate (S-40), poloxamer (poloxamer), sodium stearate, glycerin gelatine etc.; Xylitol and composites of starch, erythritol etc.The drop pill medicine is auxilliary than being 1: 1-6; During preparation there be adoptable drop pill condensing agent: vegetable oil such as liquid paraffin, dimethicone, Semen Maydis oil, kerosene or its mixture are used for water-soluble base.

Preparation method of the present invention, particularly preferred method is as follows:

In the step 1,

Extracting mode is if adopt decocting method, and method is as follows: with the 2-12 water extraction doubly of medical material weight, be 7-10 if use aqueous alkali, its pH value; Extraction time is 1-3 time, extracts 1-3 hour at every turn.

Extracting mode is if adopt the warm macerating method, and method is as follows: soaking with medical material weight 10-30 aqueous solvent doubly, is 7-10 if use aqueous alkali, its pH value; Temperature be 75 ℃-95 ℃ warm macerating 2-4 hour.

Extracting mode is if adopt continuous dynamic countercurrent extraction, and method is as follows: the medical material coarse crushing, and add water and run through, be respectively charged into 3-7 extraction pot, Continuous Countercurrent Extraction with medical material weight 3-8 aqueous solvent doubly, is 7-10 if use aqueous alkali, its pH value; Circulation countercurrent extraction 0.5-3 hour extracts 2-4 time.

Extracting mode is if adopt microwave extraction, and method is as follows: carry out microwave extraction with medical material weight 6-18 aqueous solvent doubly, as if being 7-10 with the aqueous alkali pH value; Extraction time 0.5-2 hour, microwave power 100-500W.

Extracting mode is if adopt reflux, extract,, and method is as follows:

With medical material weight 2-12 aqueous solvent reflux, extract, doubly, if be 7-10 with the aqueous alkali pH value; Extract 1-3 time, extracted 1-3 hour at every turn.

Extracting mode is if adopt percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with medical material weight 10-20 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value; Flooded percolation 2-5ml/ minute 24 hours.

Extracting mode is if adopt hyperpressure extraction, and method is as follows: medical material is crossed the 10-40 order, pressure 200-500MPa, and dwell time 3-10min extracts with medical material weight 6-18 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value, soak time 12-24 hour.

Extracting mode is if adopt the enzyme process assisted extraction, and method is as follows:

Be selected from following enzyme preparation, viscozyme L enzyme preparation, alkaline protease, cellulase, hemicellulase etc.

In the step 2,

After the extracting solution coarse filtration, concentrate or do not concentrate, last macroporous adsorbent resin behind the washing decontamination, washes out fully with ethanol water eluting to effective ingredient,

Wherein method for concentration is selected from: normal pressure concentrates, concentrating under reduced pressure or membrance concentration;

Membrance concentration wherein: use reverse osmosis membrane to concentrate, fluid temperature 10-95 ℃, pressure 0-4.5MPa,

Refined liquid after making with extra care in the step 2 can be carried out concentrate drying as required,

Wherein method for concentration is selected from: normal pressure concentrates or concentrating under reduced pressure

Wherein drying means is selected from: constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying or far-infrared ray drying;

In the step 3,

The preferred drop pill of described preparation, its mesostroma is the drop pill of xylitol and starch eutectic mixture, method is as follows: medicine: substrate=1: 3-7, add Borneolum Syntheticum again, liquid paraffin is a condensing agent, 85 ℃-95 ℃ of medicine temperature, change 1-2 hour material time, condensate temperature-2-4 ℃, drip apart from 5-10cm, drip fast per minute 30-45 and drip.

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: medicine: substrate=1: 1-6, add Borneolum Syntheticum again, and the dimethyl-silicon oil coolant, the ice-water bath cooling, 75-85 ℃ of medicine temperature dripped apart from 5-10cm, drips fast per minute 20-30 and drips.

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, and method is as follows:

Medicine: substrate=1: 1-6, add Borneolum Syntheticum again, liquid paraffin coolant, condensate temperature are below 10 ℃, and 75-85 ℃ of medicine temperature dripped apart from 5-10cm, drips fast per minute 15-25 and drips.

The most preferred preparation method of the present invention is as follows:

In the step 1,

Extracting mode is if adopt decocting method, and method is as follows: with 8 times water extraction of medical material weight, be 8 if use aqueous alkali, its pH value; Extraction time is 2 times, 2 hours for the first time, and 1 hour for the second time.

Extracting mode is if adopt the warm macerating method, and method is as follows: the aqueous solvent with 20 times of medical material weight is soaked, and is 8 if use aqueous alkali, its pH value; Temperature is 90 ℃ of warm macerating 3 hours.

Extracting mode is if adopt continuous dynamic countercurrent extraction, and method is as follows: pulverizing medicinal materials 1-8mm, and add 2 times of water and run through, be respectively charged into 5 extraction pot, 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, are 8 if use aqueous alkali, its pH value; Circulation countercurrent extraction 1.5 hours extracts 3 times.

Extracting mode is if adopt microwave extraction, and method is as follows: the aqueous solvent with 12 times of medical material weight is carried out microwave extraction, as if being 8-9 with the aqueous alkali pH value; 1 hour extraction time, microwave power 320W.

Extracting mode is if adopt reflux, extract,, and method is as follows:

With the aqueous solvent reflux, extract, of 10 times of medical material weight, if be 8 with the aqueous alkali pH value; Extract 2 times, 2 hours for the first time, 1 hour for the second time.

Extracting mode is if adopt percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with the aqueous solvent of 15 times of medical material weight, if be 8 with the aqueous alkali pH value; Flooded percolation 3ml/ minute 24 hours.

Extracting mode is if adopt hyperpressure extraction, and method is as follows: medical material is crossed 20 orders, pressure 300MPa, and dwell time 5min extracts with the aqueous solvent of 10 times of medical material weight, if be 8 with the aqueous alkali pH value, soak time 16 hours.

Be preferably viscozyme L enzyme preparation, 50 ℃, pH value 4.6, medical material granularity 20-35 order, enzyme dosage 1: 200, first step enzymolysis and extraction time 80min, solid-to-liquid ratio 1: 15, second water is carried time 40min, solid-to-liquid ratio 1: 9.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry,

Described concentrate drying preferably spray drying: 180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.

Lyophilization: pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.

Microwave drying: power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.

In the step 3,

The preferred drop pill of described preparation, its mesostroma is the drop pill of xylitol and starch eutectic mixture, method is as follows: optimized parameter: medicine: substrate=1: 5.5, add Borneolum Syntheticum again, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature, change 1.5 hours material time, 1 ℃ of condensate temperature drips apart from 7cm, drips 35 of fast per minutes.

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: optimized parameter: medicine: substrate=1: 3, add Borneolum Syntheticum again, and the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes.

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, method is as follows: optimized parameter: medicine: substrate=1: 3, add Borneolum Syntheticum again, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

The most preferred preparation method of other the present invention is listed in the embodiment of the invention.

The present invention is more more superior than prior art because of the change of technology causes the present invention to treat the medicine of coronary heart disease.

Following experimental data is used to illustrate effect of the present invention: experimental drug is the medicine of the embodiment of the invention 1.

The comparison of medicinal dropping ball aspect the angina pectoris treatment effect of table 1 treatment coronary heart disease

Group	n	Produce effects	Effectively	Invalid	Increase the weight of	Total effective rate/%
							The treatment group	49	20	28	1	0	97.9①
Matched group	49	8	29	11	1	75.5

Annotate: 1. compare, analyze U=3.039, P＜0.01 through Radit with matched group.

The comparison of medicinal dropping ball aspect ECG curative effect of table 2 treatment coronary heart disease

Group	n	Produce effects	Effectively	Invalid	Increase the weight of	Total effective rate/%
							The treatment group	49	11	20	18	0	63.2①
Matched group	49	2	17	28	2	38.7

Annotate: 1. compare, analyze U=2.685, P＜0.01 through Radit with matched group.

Hemorheology index variation before and after the medicinal dropping ball treatment of table 3 treatment coronary heart disease (x ± s)

The medicinal dropping ball medication left ventricular contractile function comparison of table 4 treatment coronary heart disease (x ± s)

Annotate: 1. compare P＞0.05 before and after the medication; 2. compare P＜0.05 before and after the medication.

Efficient, quick-acting, the multi-purpose pure Chinese medicine dripping pills preparation that the present invention adopts the modern pharmacy technology to develop, prevent and treat the coronary heart disease determined curative effect, and can improve the blood capillary circulation,, comprise that diabetic renal papillary necrosis and diabetic nephropathy all have good preventive and therapeutic effect the diabetes microvascular complication.

The invention has the advantages that: this process route can be realized industrialization, and is stable and controllable for quality.Relevant test shows, the present invention is than prior art curative effect height, the product purity height, and good absorbing, steady quality, the purposes novelty, preparation method technology is simple, easy to operate, with low cost simultaneously, is fit to suitability for industrialized production.

The specific embodiment

Specify the present invention with embodiment below, embodiment is for the ease of understanding the present invention, and claim that does not limit the present invention in any way and core content.

Embodiment 1

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%.

Preparation method:

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are extracted 2 times with the water boil of 8 times of weight, and 2 hours for the first time, 1 hour for the second time, mixed extract.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry.

Step 3, with extract and Borneolum Syntheticum together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 5.5 adds Borneolum Syntheticum, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes and be prepared into drop pill.

Embodiment 2

Prescription: Radix Salviae Miltiorrhizae 20%, Radix Notoginseng 79%, Borneolum Syntheticum 1%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the water extraction of 2 times of weight, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV60% ethanol elution is collected 60% alcohol eluen, and is concentrated or dry.

Step 3, with step 2 extract and Borneolum Syntheticum together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, liquid paraffin is a condensing agent, 85 ℃ of medicine temperature are changed 1 hour material time, condensate temperature-2 ℃, drip apart from 5cm, drip 30 of fast per minutes.

Embodiment 3

Prescription: Radix Salviae Miltiorrhizae 97%, Radix Notoginseng 2%, Borneolum Syntheticum 1.0%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the water extraction of 12 times of weight, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV70% ethanol elution is collected 70% alcohol eluen, and is concentrated or dry.

Step 3, with step 2 extract and Borneolum Syntheticum together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 7 adds Borneolum Syntheticum, liquid paraffin is a condensing agent, 95 ℃ of medicine temperature are changed 2 hours material time, 4 ℃ of condensate temperatures, drip apart from 10cm, drip 45 of fast per minutes.

Embodiment 4

Prescription: Radix Salviae Miltiorrhizae 80%, Radix Notoginseng 19.8%, Borneolum Syntheticum 0.2%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the water extraction of 12 times of weight, and extraction time is 3 times, extracts mixed extract 3 hours at every turn.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV90% ethanol elution is collected 90% alcohol eluen, and is concentrated or dry.

Step 3 uses step 2 extract and Borneolum Syntheticum together as active constituents of medicine, and substrate is pool Luo Samu, and rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, dimethyl-silicon oil coolant, ice-water bath cooling, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 20 of fast per minutes.

Embodiment 5

Prescription: Radix Salviae Miltiorrhizae 27%, Radix Notoginseng 70%, Borneolum Syntheticum 3.0%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the potass extraction of 2 times of weight, and the aqueous alkali pH value is 7, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV65% ethanol elution is collected 65% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is pool Luo Samu, and rate of charge is: the extract medicine: substrate=1: 6, add Borneolum Syntheticum, dimethyl-silicon oil coolant, ice-water bath cooling, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 30 of fast per minutes.

Embodiment 6

Prescription: Radix Salviae Miltiorrhizae 94%, Radix Notoginseng 4%, Borneolum Syntheticum 2%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the potass extraction of 2 times of weight, and the aqueous alkali pH value is 10, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

In the step 2, extracting solution is evaporated to 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV75% ethanol elution is collected 75% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate for the PEG1000 rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 7

Prescription: Radix Salviae Miltiorrhizae 63%, Radix Notoginseng 35%, Borneolum Syntheticum 2%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are soaked with the aqueous solvent of 10 times of weight, and temperature is 75 ℃ of warm macerating 2 hours.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV85% ethanol elution is collected 85% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG1000, rate of charge is: the extract medicine: substrate=1: 6 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 8

Prescription: Radix Salviae Miltiorrhizae 69.5%, Radix Notoginseng 30%, Borneolum Syntheticum 0.5%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are soaked with the aqueous solvent of 30 times of weight, and temperature is 95 ℃ of warm macerating 4 hours.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG4000, and rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 9

Prescription: Radix Salviae Miltiorrhizae 90%, Radix Notoginseng 9%, Borneolum Syntheticum 1%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing add water and run through, and are respectively charged into 3 extraction pot, Continuous Countercurrent Extraction, with the aqueous solvent of 3 times of medical material weight, circulation countercurrent extraction 0.5 hour extracts 2 times.

In the step 2, extracting solution is concentrated into 1.5 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG4000, rate of charge is: the extract medicine: substrate=1: 6 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 10

Prescription: Radix Salviae Miltiorrhizae 75.2%, Radix Notoginseng 23.5%, Borneolum Syntheticum 1.3%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing add water and run through, and are respectively charged into 7 extraction pot, Continuous Countercurrent Extraction, with the aqueous solvent of 8 times of medical material weight, circulation countercurrent extraction 3 hours extracts 4 times.

In the step 2, extracting solution is concentrated into 1.5 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG6000, and rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 11

Prescription: Radix Salviae Miltiorrhizae 78.5%, Radix Notoginseng 21%, volatile oil of Lignum Dalbergiae Odoriferae 0.5%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng carry out microwave extraction with the aqueous solvent of 6 times of weight, 0.5 hour extraction time, microwave power 100W.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and volatile oil of Lignum Dalbergiae Odoriferae are together as active constituents of medicine, substrate is PEG6000, rate of charge is: the extract medicine: substrate=1: 6 adds volatile oil of Lignum Dalbergiae Odoriferae, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 12

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng carry out microwave extraction with the aqueous solvent of 18 times of weight, 2 hours extraction times, microwave power 500W.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 1 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 13

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Styrax 0.92%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng carry out microwave extraction with the aqueous alkali of 18 times of weight, and the aqueous alkali pH value is 7-10; 2 hours extraction times, microwave power 500W.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1.5BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Styrax are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 6 adds Styrax, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 14

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Rhizoma Chuanxiong extract 0.92%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng extract 2 times with 2 times aqueous solvent reflux, extract,, extract 1 hour at every turn.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 2BV purified water, the 2.5BV95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Rhizoma Chuanxiong extract are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 6 adds Rhizoma Chuanxiong extract, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 15

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with 12 times aqueous alkali reflux, extract,, and the aqueous alkali pH value is 7; Extract 3 times, extracted 3 hours at every turn.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.0BV 95% ethanol elution is collected 95% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 6 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 16

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with 10 times aqueous alkali reflux, extract,, and the aqueous alkali pH value is 10; Extract 3 times, extracted 1 hour at every turn.

In the step 2, extracting solution is concentrated into 1.5 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV95% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry.

Embodiment 17

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing, with the alkaline soak of 10 times of described medical material weight, the aqueous alkali pH value is 7; Flooded percolation 2ml/ minute 24 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.5BV 80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry.

Embodiment 18

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing, with the alkaline soak of 20 times of described medical material weight, the aqueous alkali pH value is 10; Flooded percolation 5ml/ minute 24 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1.5BV purified water, 2.5BV 80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry.

Embodiment 19

Step 1, hyperpressure extraction: Radix Salviae Miltiorrhizae, Radix Notoginseng cross 10 orders, pressure 200MPa, dwell time 3min, the potass extraction that the weight of usefulness Radix Salviae Miltiorrhizae and Radix Notoginseng is 6 times, the aqueous alkali pH value is 7, soak time 12 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2BV purified water, 2.5BV 80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is the drop pill of pool Luo Samu, rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes.

Embodiment 20

Step 1, hyperpressure extraction: Radix Salviae Miltiorrhizae, Radix Notoginseng cross 40 orders, pressure 500MPa, dwell time 10min, the potass extraction that usefulness Radix Salviae Miltiorrhizae and three seven weight are 18 times, the aqueous alkali pH value is 10, soak time 24 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.5BV 80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry.

Embodiment 21

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, volatile oil of Lignum Dalbergiae Odoriferae 0.92%.

Step 1, extracting mode is if adopt the enzyme process assisted extraction, and method is as follows:

Be selected from following enzyme preparation, viscozyme L enzyme preparation, alkaline protease, cellulase, hemicellulase.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.5BV 75% ethanol elution is collected 75% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and volatile oil of Lignum Dalbergiae Odoriferae are together as active constituents of medicine, substrate is pool Luo Samu, rate of charge is: the extract medicine: substrate=1: 6 adds volatile oil of Lignum Dalbergiae Odoriferae, the dimethyl-silicon oil coolant, the ice-water bath cooling, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 30 of fast per minutes.

Embodiment 22

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are soaked with the aqueous solvent of 20 times of weight, and temperature is 90 ℃ of warm macerating 3 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.5BV 85% ethanol elution is collected 85% alcohol eluen, and is concentrated or dry.

Embodiment 23

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng are pulverized 1-8mm, add 2 times of water and run through, and are respectively charged into 5 extraction pot, and 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, and circulation countercurrent extraction 1.5 hours extracts 3 times.

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.5BV 85% ethanol elution is collected 85% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Styrax are together as active constituents of medicine, substrate is the drop pill of pool Luo Samu, rate of charge is: the extract medicine: substrate=1: 3 adds Styrax, the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes.

Embodiment 24

Step on step 1, carry out microwave extraction, if be 8-9 with the aqueous alkali pH value with the aqueous solvent of 12 times of Radix Salviae Miltiorrhizaes, pseudo-ginseng weight; 1 hour extraction time, microwave power 320W.

In the step 2, extracting solution is concentrated into 1.5 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.5BV 85% ethanol elution is collected 85% alcohol eluen, and is concentrated or dry.

Step 3, extract that obtains with step 2 and Rhizoma Chuanxiong extract be together as active constituents of medicine, substrate

Be the drop pill of pool Luo Samu, rate of charge is: the extract medicine: substrate=1: 3 adds Rhizoma Chuanxiong extract, two

The methyl-silicone oil condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes.

Embodiment 25

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are used the aqueous solvent reflux, extract, of 10 times of medical material weight, extract 2 times, 2 hours for the first time, 1 hour for the second time

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301,1BV purified water

After the flushing, 2.0BV 85% ethanol elution is collected 85% alcohol eluen, and is concentrated or dry.

Embodiment 26

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are used the aqueous alkali reflux, extract, of 10 times of medical material weight, and aqueous alkali ph=8 extracts 2 times, and 2 hours for the first time, 1 hour for the second time

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1.5BV purified water, 2.0BV 85% ethanol elution is collected 85% alcohol eluen, and is concentrated or dry.

Embodiment 27

Prescription: Radix Salviae Miltiorrhizae 77%, Radix Notoginseng 22%, Borneolum Syntheticum 1%.

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng coarse crushing are soaked with the aqueous solvent of 15 times of medical material weight, flood percolation 3ml/ minute 24 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, 2.5BV 70% ethanol elution is collected 70% alcohol eluen, and is concentrated or dry.

Embodiment 28

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng coarse crushing, with the alkaline soak of 15 times of medical material weight, the aqueous alkali pH value is 8; Flooded percolation 3ml/ minute 24 hours.

Embodiment 29

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng are crossed 20 orders, pressure 300MPa, dwell time 5min extracts with the aqueous solvent of 10 times of medical material weight, if be 8 with the aqueous alkali pH value, soak time 16 hours.

Embodiment 30

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng are crossed 20 orders, pressure 300MPa, dwell time 5min, with the potass extraction of 10 times of medical material weight, the aqueous alkali pH value is 8, soak time 16 hours.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.0BV 65% ethanol elution is collected 65% alcohol eluen, and concentrating under reduced pressure becomes extractum.

Embodiment 31

Step 1 adopts the enzyme process assisted extraction: viscozyme L enzyme preparation, 50 ℃, pH value 4.6, Radix Salviae Miltiorrhizae, pseudo-ginseng granularity 20-35 order, medical material: enzyme dosage 1: 200, first step enzymolysis and extraction time 80min, solid-liquid weight ratio 1: 15, second water is carried time 40min, solid-liquid weight ratio 1: 9.

In the step 2, extracting solution is concentrated into 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.0BV 70% ethanol elution is collected 70% alcohol eluen, and concentrating under reduced pressure becomes extractum.

Embodiment 32

In the step 2, extracting solution is evaporated to 1.5 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.0BV 70% ethanol elution is collected 70% alcohol eluen, is condensed into extractum.

Embodiment 33

In the step 2, extracting solution is evaporated to 1.3 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.0BV 70% ethanol elution is collected 70% alcohol eluen, and is concentrated or dry.

Embodiment 34

In the step 2, extracting solution is evaporated to 1.8 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.0BV 70% ethanol elution is collected 70% alcohol eluen, and concentrating under reduced pressure becomes extractum.

Embodiment 35

In the step 2, extracting solution uses reverse osmosis membrane to concentrate (fluid temperature 10-95 ℃, pressure 0-4.5MPa) to 2 times of amounts of medical material, last macroporous adsorbent resin D301, after the flushing of 2.5BV purified water, 2.0BV 70% ethanol elution, collect 70% alcohol eluen, concentrating under reduced pressure becomes extractum.

Embodiment 36

Embodiment 37

In the step 2, extracting solution uses reverse osmosis membrane to concentrate (fluid temperature 10-95 ℃, pressure 0-4.5MPa) to 2 times of amounts of medical material, last macroporous adsorbent resin D301, and after the flushing of 2.5BV purified water, 2.0BV 80% ethanol elution is collected 80% alcohol eluen, is condensed into extractum.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG1000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 38

In the step 2, extracting solution uses reverse osmosis membrane to concentrate (fluid temperature 10-95 ℃, pressure 0-4.5MPa) to 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV80% ethanol elution is collected 80% alcohol eluen, and concentrating under reduced pressure becomes extractum.

Embodiment 39

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV80% ethanol elution is collected 80% alcohol eluen, is condensed into extractum

Embodiment 40

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, and last macroporous adsorbent resin D301 is after the flushing of 1BV purified water, the 2.5BV80% ethanol elution is collected 80% alcohol eluen, concentrating under reduced pressure, spray drying: (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.)

Embodiment 41

In the step 2, extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.0BV80% ethanol elution is collected 80% alcohol eluen, concentrates spray drying: (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.)

Embodiment 42

In the step 2, extracting solution uses reverse osmosis membrane to concentrate (fluid temperature 10-95 ℃, pressure 0-4.5MPa) to 1 times of amount of medical material, last macroporous adsorbent resin D301 is after the flushing of 1.5BV purified water, the 2.5BV80% ethanol elution, collect 80% alcohol eluen, concentrating under reduced pressure, spray drying: (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.)

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG4000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 43

In the step 2, extracting solution is evaporated to 1 times of amount of medical material, and last macroporous adsorbent resin D301 is after the flushing of 1BV purified water, 2.5BV 70% ethanol elution is collected 70% alcohol eluen, concentrating under reduced pressure, spray drying: (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.)

Embodiment 44

In the step 2, extracting solution is evaporated to 1 times of amount of medical material, and last macroporous adsorbent resin D301 is after the flushing of 1BV purified water, 2.5BV 65% ethanol elution is collected 65% alcohol eluen, concentrating under reduced pressure, spray drying: (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.)

Embodiment 45

In the step 2, the extracting solution normal pressure is concentrated into 1 times of amount of medical material, and last macroporous adsorbent resin D301 is after the flushing of 1BV purified water, the 2.5BV80% ethanol elution, collect 80% alcohol eluen, concentrating under reduced pressure, lyophilization: (pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.)

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG6000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 46

In the step 2, the extracting solution normal pressure is concentrated into 1.5 times of amounts of medical material, and last macroporous adsorbent resin D301 is after the flushing of 1BV purified water, the 2.5BV80% ethanol elution, collect 80% alcohol eluen, concentrating under reduced pressure, lyophilization: (pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.)

Embodiment 47

Step 1 is carried out microwave extraction with the aqueous solvent of 12 times of Radix Salviae Miltiorrhizaes, pseudo-ginseng weight, if be 8-9 with the aqueous alkali pH value; 1 hour extraction time, microwave power 320W.

In the step 2, extracting solution uses reverse osmosis membrane to concentrate (fluid temperature 10-95 ℃, pressure 0-4.5MPa) to 1.5 times of amounts of medical material, concentrate and go up macroporous adsorbent resin D301, after the flushing of 1BV purified water, the 2.0BV80% ethanol elution is collected 80% alcohol eluen, concentrating under reduced pressure, lyophilization: (pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.)

Embodiment 48

In the step 2, extracting solution is evaporated to 2 times of amounts of medical material, and last macroporous adsorbent resin D301 is after the flushing of 1BV purified water, the 2.5BV80% ethanol elution, collect 80% alcohol eluen, concentrating under reduced pressure, lyophilization: (pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.)

Embodiment 49

Step 1, Radix Salviae Miltiorrhizae and pseudo-ginseng pulverize separately 1-8mm add 2 times of water and run through, and are respectively charged into 5 extraction pot, and 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, and circulation countercurrent extraction 1.5 hours extracts 3 times.

In the step 2, Radix Salviae Miltiorrhizae and Radix Notoginseng extracting solution are evaporated to 1 times of amount of medical material respectively, last macroporous adsorbent resin D301, after the flushing of 1BV purified water, the 2.5BV80% ethanol elution, collect 80% alcohol eluen, concentrating under reduced pressure, microwave drying: (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Step 3, Radix Salviae Miltiorrhizae that obtains with step 2 and Radix Notoginseng extract and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG6000, rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 50

Step 1, Radix Salviae Miltiorrhizae and Radix Notoginseng are soaked with the aqueous solvent of 20 times of weight respectively, and temperature is 90 ℃ of warm macerating 3 hours.

In the step 2, Radix Salviae Miltiorrhizae and Radix Notoginseng extracting solution are concentrated into 1.5 times of amounts of medical material respectively, last macroporous adsorbent resin D301, after the flushing of 1BV purified water, the 2.5BV70% ethanol elution, collect 70% alcohol eluen, concentrating under reduced pressure, microwave drying: (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Embodiment 51

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make tablet.

Embodiment 52

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make capsule.

Embodiment 53

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make granule.

Embodiment 54

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make powder.

Embodiment 55

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make pill.

Claims

1. compound red sage root preparation is characterized in that prescription consists of: Radix Salviae Miltiorrhizae 20%-97%, and Radix Notoginseng 2.0%-79%, Borneolum Syntheticum 0.2%-3.0%, its preparation method is as follows:

Step 2, extracting solution separates with macroporous resin, obtains extract;

Step 3, said extracted thing and Borneolum Syntheticum are prepared into pharmaceutical preparation together as active constituents of medicine.

2. the preparation of claim 1 is characterized in that, prescription consists of: Radix Salviae Miltiorrhizae 63.0%-94%, and Radix Notoginseng 4.0%-35.0%, Borneolum Syntheticum 0.5%-2.0%, its preparation method is as follows:

In the step 1, described Radix Salviae Miltiorrhizae, pseudo-ginseng are medical material, decoction pieces or the powder of Radix Salviae Miltiorrhizae, Radix Notoginseng, and the described water that is extracted as is carried, and extract to adopt Radix Salviae Miltiorrhizae, panax mixed to extract, or Radix Salviae Miltiorrhizae, Radix Notoginseng extract separately respectively, extract the back and mix;

In the step 2, described with the macroporous resin separation, be extracting solution to be separated through macroporous resin purify;

In the step 3, the extract of step 2 and Borneolum Syntheticum are prepared into dropping pill formulation together as active constituents of medicine.

3. the preparation of claim 1 is characterized in that, prescription consists of: Radix Salviae Miltiorrhizae 75.2%-90%, and Radix Notoginseng 9%-23.5%, Borneolum Syntheticum 0.5%-1.3%, its preparation method is as follows:

In the step 1, described extracting mode is selected from decocting method, warm macerating method, enzyme process assisted extraction, continuous dynamic countercurrent extraction, reflux, extract,, percolation extraction, microwave extraction or hyperpressure extraction,

In the step 2, extracting solution concentrates or does not concentrate, and uses macroporous resin to separate remove impurity, and macroporous resin is the macroporous resin of nonpolar or low pole;

Refined liquid after refining is through concentrating or/and drying obtains extract, and extract is extractum or powder;

Method for concentration is selected from normal pressure and concentrates or concentrating under reduced pressure,

Drying means is selected from constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying or far-infrared ray drying,

In the step 3, described dropping pill formulation, the adjuvant that adopts during preparation is selected from: water-soluble base has Polyethylene Glycol, polyoxyethylene monostearate, poloxamer, sodium stearate, glycerin gelatine; Xylitol and composites of starch, erythritol, the drop pill medicine is auxilliary than being 1: 1-6; The drop pill condensing agent that adopts during preparation is selected from: liquid paraffin, dimethicone, Semen Maydis oil, kerosene, vegetable oil or its mixture.

4. the preparation of claim 3 is characterized in that, prescription consists of: Radix Salviae Miltiorrhizae 82.87%, and Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%,, its preparation method is as follows:

In the step 1,

Extracting mode adopts decocting method, and method is as follows: with medical material weight 2-12 water extraction doubly, be 7-10 if use aqueous alkali, its pH value; Extraction time is 1-3 time, extracts 1-3 hour at every turn;

Extracting mode adopts the warm macerating method, and method is as follows: soak with medical material weight 10-30 aqueous solvent doubly, temperature be 75 ℃-95 ℃ warm macerating 2-4 hour;

Extracting mode adopts continuous dynamic countercurrent extraction, and method is as follows: the medical material coarse crushing, and add water and run through, be respectively charged into 3-7 extraction pot, Continuous Countercurrent Extraction, with medical material weight 3-8 aqueous solvent doubly, circulation countercurrent extraction 0.5-3 hour extracts 2-4 time;

Extracting mode adopts microwave extraction, and method is as follows: carry out microwave extraction with medical material weight 6-18 aqueous solvent doubly, as if being 7-10 with the aqueous alkali pH value; Extraction time 0.5-2 hour, microwave power 100-500W;

Extracting mode adopts reflux, extract,, and method is as follows:

With medical material weight 2-12 aqueous solvent reflux, extract, doubly, if be 7-10 with the aqueous alkali pH value; Extract 1-3 time, extracted 1-3 hour at every turn;

Extracting mode adopts percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with medical material weight 10-20 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value; Flooded percolation 2-5ml/ minute 24 hours;

Extracting mode adopts hyperpressure extraction, and method is as follows: medical material is crossed the 10-40 order, pressure 200-500MPa, and dwell time 3-10min extracts with medical material weight 6-18 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value, soak time 12-24 hour;

Extracting mode adopts the enzyme process assisted extraction, and method is as follows:

Enzyme preparation is selected from, viscozyme L enzyme preparation, alkaline protease, cellulase, hemicellulase;

In the step 2,

In the step 3,

Described preparation drop pill, its mesostroma is the drop pill of xylitol and starch eutectic mixture, preparation method is: medicine and substrate are mixed, and medicine: substrate=1: 3-7 adds Borneolum Syntheticum again, liquid paraffin is a condensing agent, 85 ℃-95 ℃ of medicine temperature are changed 1-2 hour material time, condensate temperature-2-4 ℃, drip apart from 5-10cm, drip fast per minute 30-45 and drip;

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: medicine and substrate are mixed, medicine: substrate=1: 1-6, add Borneolum Syntheticum again, and the dimethyl-silicon oil coolant, the ice-water bath cooling, 75-85 ℃ of medicine temperature dripped apart from 5-10cm, drips fast per minute 20-30 and drips;

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, method is as follows: medicine and substrate are mixed, medicine: substrate=1: 1-6, add Borneolum Syntheticum again, liquid paraffin coolant, condensate temperature be below 10 ℃, 75-85 ℃ of medicine temperature, drip apart from 5-10cm, drip fast per minute 15-25 and drip.

5. the preparation of claim 4 is characterized in that, wherein:

In the step 1,

Extracting mode adopts decocting method, and method is as follows: with the water extraction of 8 times of medical material weight, be 8 if use aqueous alkali, its pH value; Extraction time is 2 times, 2 hours for the first time, and 1 hour for the second time;

Extracting mode adopts the warm macerating method, and method is as follows: the aqueous solvent with 20 times of medical material weight is soaked, and temperature is 90 ℃ of warm macerating 3 hours;

Extracting mode adopts continuous dynamic countercurrent extraction, and method is as follows: pulverizing medicinal materials 1-8mm, and add 2 times of water and run through, be respectively charged into 5 each extraction pot, 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, and circulation countercurrent extraction 1.5 hours extracts 3 times;

Extracting mode adopts microwave extraction, and method is as follows: the aqueous solvent with 12 times of medical material weight is carried out microwave extraction, as if being 8-9 with the aqueous alkali pH value; 1 hour extraction time, microwave power 320W;

Extracting mode adopts reflux, extract,, and method is as follows:

With the aqueous solvent reflux, extract, of 10 times of medical material weight, if be 8 with the aqueous alkali pH value; Extract 2 times, 2 hours for the first time, 1 hour for the second time;

Extracting mode adopts percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with the aqueous solvent of 15 times of medical material weight, if be 8 with the aqueous alkali pH value; Flooded percolation 3ml/ minute 24 hours;

Extracting mode adopts hyperpressure extraction, and method is as follows: medical material is crossed 20 orders, pressure 300MPa, and dwell time 5min extracts with the aqueous solvent of 10 times of medical material weight, if be 8 with the aqueous alkali pH value, soak time 16 hours;

With viscozyme L enzyme preparation, 50 ℃ of temperature, pH value 4.6, medical material granularity 20-35 order, enzyme dosage 1: 200, first step enzymolysis and extraction time 80min, solid-to-liquid ratio 1: 15, second water is carried time 40min, solid-to-liquid ratio 1: 9;

Described drying is a spray drying: 180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10 or lyophilization: pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃; Or microwave drying: power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40;

In the step 3,

Described preparation drop pill, its mesostroma is the drop pill of xylitol and starch eutectic mixture, method is as follows: medicine and substrate are mixed, and medicine: substrate=1: 5.5 adds Borneolum Syntheticum again, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes;

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: medicine and substrate are mixed, medicine: substrate=1: 3, add Borneolum Syntheticum again, and the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes;

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, method is as follows: medicine and substrate are mixed, medicine: substrate=1: 3, add Borneolum Syntheticum again, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature, drip apart from 6cm, drip 20 of fast per minutes.

6. the preparation of claim 1, it is characterized in that, wherein: the pharmaceutical formulation of claim 1 is that the blood circulation promoting and blood stasis dispelling according to the traditional Chinese medical science, the effect of regulating QI to relieve pain are determined, situation according to patient, wherein Borneolum Syntheticum is with being selected from volatile oil of Lignum Dalbergiae Odoriferae, Styrax, Rhizoma Chuanxiong extract, or wherein any combination, or other invigorating blood circulation (breaking) silt, regulating QI to relieve pain medicine or pharmaceutical preparations substitute.

7. the preparation of claim 1, it is characterized in that wherein: preparation formulation is selected from: tablet, capsule, drop pill, pill, powder spray, aerosol, gel, injection, lyophilizing divide the quick releasing formulation or the durative action preparation of injection, Orally-disintegrating tablet and these dosage forms.

8. the application of the preparation of claim 1 in the medicine of preparation resisting coronary heart disease, angina pectoris cardiovascular disease.

9. the preparation method of the preparation of claim 1, wherein the compound red sage root preparation prescription is: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%, its preparation method is:

In the step 1, described Radix Salviae Miltiorrhizae, pseudo-ginseng are medical material, decoction pieces or the powder of Radix Salviae Miltiorrhizae, Radix Notoginseng, and the described water that is extracted as is carried, and described extraction adopts Radix Salviae Miltiorrhizae, panax mixed to extract, or Radix Salviae Miltiorrhizae, Radix Notoginseng extract separately respectively, extract the back and mix;

In the step 2, after the extracting solution coarse filtration, concentrate or do not concentrate, last macroporous adsorbent resin behind the washing decontamination, washes out fully with ethanol water eluting to effective ingredient, and macroporous resin is the macroporous resin of nonpolar or low pole;

10. the preparation method of claim 9, wherein

In the step 1,

Extracting mode adopts decocting method, and method is as follows: with the water extraction of 8 times of medical material weight, be 8 if use aqueous alkali, its pH value; Extraction time is 2 times, 2 hours for the first time, and 1 hour for the second time,

In the step 2,

Extracting solution is concentrated into 1 times of amount of medical material, last macroporous adsorbent resin D301, and after the flushing of 1BV purified water, the 2.5BV80% ethanol elution is collected 80% alcohol eluen, and is concentrated or dry,

In the step 3,

Substrate is PEG1000, PEG4000, PEG6000, PEG10000 or their mixing, and method is as follows: medicine and substrate are mixed, medicine: substrate=1: 3, add Borneolum Syntheticum again, use the liquid paraffin coolant, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.