CN102119962A

CN102119962A - Extract for preventing and treating angina pectoris and preparation method and application thereof

Info

Publication number: CN102119962A
Application number: CN2010100313135A
Authority: CN
Inventors: 刘岩; 徐波; 刘顺航; 戚可人; 陈红; 丛德刚; 赵国辉
Original assignee: TIANJIN TASLY MODERN CHINESE MEDICINE RESOURCE CO Ltd
Current assignee: TIANJIN TASLY MODERN CHINESE MEDICINE RESOURCE CO Ltd
Priority date: 2010-01-07
Filing date: 2010-01-07
Publication date: 2011-07-13

Abstract

The invention relates to an extract for preventing and treating angina pectoris and a preparation method and application thereof. The preparation method provided by the invention comprises the following steps of: 1, extracting red sage root and notoginseng to obtain extracted solution; 2, ultrafiltering the extracted solution to obtain extract; and 3, preparing a pharmaceutical preparation from the extract and borneol as the active components of the medicament.

Description

Extract of a kind of prevention and treatment angina pectoris and preparation method thereof and purposes

Technical field:

The present invention relates to the process for purification of the extracting method of cardiovascular drugs compound red sage root preparation, particularly extract.

Background technology:

Compound red sage root preparation is made up of Radix Salviae Miltiorrhizae, Radix Notoginseng, Borneolum Syntheticum etc., the effect that blood circulation promoting and blood stasis dispelling, regulating QI to relieve pain are arranged, compound red sage root preparation prolonged application in the clinical disease that has a microcirculation disturbance, evident in efficacy in the treatment of Medicine Systems disease such as cardiovascular and cerebrovascular disease, respiratory system disease, digestive system disease, renal system disease etc.Therefore purposes more and more widely.In recent years, except that the Medicine Systems disease, also extensively adopt at department of dermatologry.

The main aspect of the therapeutical effect of compound red sage root preparation is: coronary heart disease, angina pectoris and microcirculation

Patients with coronary heart disease nail fold microcirculation shows tangible circulatory disturbance.Systemic microcirculation can think that the nail fold microcirculation is whole circulation window by the performance of nail fold microcirculation.

Shen shapes etc. use compound red sage root preparation to treat to 62 routine patients with coronary heart disease.These patient's nail fold pipe loop decreased number, pipe loop intersection deformity increases, and pipe loop diameter reduces, and blood flow rate slows down, the minimizing of line grain stream, erythrocyte gathers obviously.The microcirculatory this abnormal change of nail fold, proved that patients with coronary heart disease exists cardiac microcirculation disturbance, after above patient treated through compound red sage root preparation, the microcirculatory every index of nail fold all had clear improvement and takes a turn for the better, the total effective rate of clinical treatment reaches 91%, and ECG improvement rate is 72%.

Li Feng etc. are divided into routine of western medicine treatment group (oral sorbitrate, quiet polarized solution add Energy mixture) and composite salvia dropping pill for curing group (except that conventional western medicine with 90 routine angina pectoris patients, add the red ball of compound Salviae Miltiorrhizae, each 10, every day 3 times) totally 20 day, the angina of drop pill group treatment as a result total effective rate 92.2% is significantly higher than routine of western medicine treatment group 68.0% (P＜0.05).Drop pill group ECG effective percentage 74.4% also is significantly higher than routine of western medicine group 48.0% (P＜0.05), and the drop pill group all significantly improves (P＜0.01) than the Western medicine group to the remission rate of symptoms such as patient's simultaneous phenomenon such as uncomfortable in chest, cardiopalmus, limb fiber crops, insomnia.

Red sage formulation mainly contains in the pharmacological action aspect the treatment cardiovascular disease:

(1) dilating coronary blood vessel improves coronary blood flow

(2) microcirculation improvement obstacle

(3) improve hemorheological property

(4) anticoagulant

(5) resisting oxygen lack

(6) anti peroxidation of lipid and elimination free radical

The preparation method of existing compound red sage root preparation is a lot, carries alcohol extraction, water extract-alcohol precipitation, alcohol extracting-water precipitating etc., many Chinese patents of also having applied for as water.

Extract is not carried out effective ingredient monitoring and purified step in the prior art, to such an extent as to existing compound red sage root preparation lacks means on quality control, the content of active ingredient still remains to be improved.

Summary of the invention:

The invention provides a kind of extracting method of compound red sage root preparation and the compound red sage root preparation for preparing by this method.

Compound red sage root preparation of the present invention is prepared from by following raw materials by weight percent medicine,

Radix Salviae Miltiorrhizae 20%-97%, Radix Notoginseng 2.0%-79%, Borneolum Syntheticum 0.2%-3.0%.

Preferred Radix Salviae Miltiorrhizae 63.0%-94% Radix Notoginseng 4.0%-35.0% Borneolum Syntheticum 0.5%-2.0%.

Preferred Radix Salviae Miltiorrhizae 75.2%-90% Radix Notoginseng 9%-23.5% Borneolum Syntheticum 0.5%-1.3%.

Most preferred Radix Salviae Miltiorrhizae 82.87% Radix Notoginseng 16.21% Borneolum Syntheticum 0.92%.

Above pharmaceutical formulation is that the blood circulation promoting and blood stasis dispelling according to the traditional Chinese medical science, the effect of regulating QI to relieve pain are determined, situation according to patient, wherein Borneolum Syntheticum can substitute with other drug or pharmaceutical preparations, as volatile oil of Lignum Dalbergiae Odoriferae, Styrax, Rhizoma Chuanxiong extract, or wherein any compositions, or other invigorating blood circulation (breaking) are become silted up, the regulating QI to relieve pain medicine.Do not affect the treatment, therefore, the increase of these prescriptions all belongs to scope of the present invention.

Compound red sage root preparation of the present invention, preparation formulation is selected from: the quick releasing formulation of tablet, capsule, drop pill, pill and these dosage forms thereof or durative action preparation, or powder spray, aerosol, gel, injection, lyophilizing divide injection, Orally-disintegrating tablet etc.Preferred dosage form is a drop pill.

According to the galenic pharmacy needs, can add during preparation pharmaceutic adjuvant as: starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, methyl starch sodium, cross-linking sodium carboxymethyl cellulose, crospolyvinylpyrrolidone etc. are separately or the various adjuvants that are mixed and made into.

The present invention also comprises with the application of composite salvia dropping pill of the present invention in the medicine of cardiovascular disease such as a kind of resisting coronary heart disease of preparation, angina pectoris.

The preparation of compound red sage root preparation of the present invention is earlier crude drug to be prepared into active constituents of medicine through following steps, further is prepared into pharmaceutical preparation again.

Preparation method of the present invention, method is as follows:

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng extract, and get extracting solution;

Step 2, extract refining obtains extract;

Step 3 together as active constituents of medicine, is prepared into pharmaceutical preparation with the galenic pharmacy routine techniques with extract and Borneolum Syntheticum.

Preparation method of the present invention, preferable methods is as follows:

In the step 1, described Radix Salviae Miltiorrhizae, pseudo-ginseng are the products as the raw material of medication preparation of the forms such as medical material, decoction pieces or powder of Radix Salviae Miltiorrhizae, Radix Notoginseng, can buy from the market, also can process according to pharmacopeia, the described water that is extracted as is carried, described extraction can adopt Radix Salviae Miltiorrhizae, panax mixed to extract, or Radix Salviae Miltiorrhizae, Radix Notoginseng extract separately respectively, extracts the back and mixes.

In the step 2, the described refining ultrafiltration that is, described ultrafiltration: be that extracting solution is purified through ultrafiltration membrance filter.

In the step 3, the extract of step 2 and Borneolum Syntheticum are prepared into dropping pill formulation together as active constituents of medicine with the galenic pharmacy routine techniques.

Preparation method of the present invention, preferred method is as follows:

In the step 1, described extracting mode is selected from decocting method, warm macerating method, enzyme process assisted extraction, continuous dynamic countercurrent extraction, reflux, extract,, percolation extraction, microwave extraction or hyperpressure extraction etc.

In the step 2, the described refining ultrafiltration that is, described ultrafiltration: be with extracting solution ultrafiltration remove impurity after predefecation is handled,

Predefecation is handled and be can be the independent or use in conjunction of following method: general material such as gauze, tiffany etc. carry out coarse filtration; The material such as the ceramic membrane of specialty carry out microfiltration; Separation of supernatant behind high speed centrifugation;

Ultrafilter membrane can be cellulose diacetate film (CA), three cellulose acetate membrane (CTA), cyanoethyl cellulose film (CN-CA), polysulfone membrane (PS), sulfonated polysulfone membrane (SPS), poly (ether sulfone) film (PES), sulfonated polyether sulfone film (SPES), polysulfonamides film (PSA), phenolphthalein side group polyarylsulfone (PAS) film (PDS), polyvinylidene fluoride film (PVDF), polyacrylonitrile film (PAN), polyimide film (N), cellulose membrane, methyl methacrylate-acrylonitrile copolymer film (MMA-NA), polyacrylonitrile/cellulose diacetate (PAN/CA) blend film, the dynamically ultrafilter membrane that forms, and the Modified Membrane of above-mentioned film.

Refined liquid after refining is through concentrating or drying obtains extract, and extract can be extractum (concentrate) or powder (dry);

Method for concentration is that normal pressure concentrates, concentrating under reduced pressure, membrance concentration etc.

Drying means is constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying, far-infrared ray drying etc.

In the step 3, described dropping pill formulation, during preparation there be adoptable adjuvant: water-soluble base has Polyethylene Glycol (PEG), polyoxyethylene monostearate (S-40), poloxamer (poloxamer), sodium stearate, glycerin gelatine etc.; Xylitol and composites of starch, erythritol etc.The drop pill medicine is auxilliary than being 1: 1-6; During preparation there be adoptable drop pill condensing agent: vegetable oil such as liquid paraffin, dimethicone, Semen Maydis oil, kerosene or its mixture are used for water-soluble base.

Preparation method of the present invention, particularly preferred method is as follows:

In the step 1,

Extracting mode is if adopt decocting method, and method is as follows: with the 2-12 water extraction doubly of medical material weight, be 7-10 if use aqueous alkali, its pH value; Extraction time is 1-3 time, extracts 1-3 hour at every turn.

Extracting mode is if adopt the warm macerating method, and method is as follows: soaking with medical material weight 10-30 aqueous solvent doubly, is 7-10 if use aqueous alkali, its pH value; Temperature be 75 ℃-95 ℃ warm macerating 2-4 hour.

Extracting mode is if adopt continuous dynamic countercurrent extraction, and method is as follows: the medical material coarse crushing, and add water and run through, be respectively charged into 3-7 extraction pot, Continuous Countercurrent Extraction with medical material weight 3-8 aqueous solvent doubly, is 7-10 if use aqueous alkali, its pH value; Circulation countercurrent extraction 0.5-3 hour extracts 2-4 time.

Extracting mode is if adopt microwave extraction, and method is as follows: carry out microwave extraction with medical material weight 6-18 aqueous solvent doubly, as if being 7-10 with the aqueous alkali pH value; Extraction time 0.5-2 hour, microwave power 100-500W.

Extracting mode is if adopt reflux, extract,, and method is as follows:

With medical material weight 2-12 aqueous solvent reflux, extract, doubly, if be 7-10 with the aqueous alkali pH value; Extract 1-3 time, extracted 1-3 hour at every turn.

Extracting mode is if adopt percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with medical material weight 10-20 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value; Flooded percolation 2-5ml/ minute 24 hours.

Extracting mode is if adopt hyperpressure extraction, and method is as follows: medical material is crossed the 10-40 order, pressure 200-500MPa, and dwell time 3-10min extracts with medical material weight 6-18 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value, soak time 12-24 hour.

Extracting mode is if adopt the enzyme process assisted extraction, and method is as follows:

Be selected from following enzyme preparation, viscozyme L enzyme preparation, alkaline protease, cellulase, hemicellulase etc.

In the step 2,

After extracting solution was used the gauze coarse filtration, refining mode adopted ultrafiltration, and method is as follows: adopting the molecular weight that dams is the ultrafilter membrane of 6000-80000; Ultrafiltration both can be adopted cross flow filter, also can adopt dead-end filtration; The pH value of feed liquid is controlled at 5-9.

Preferred ultrafilter membrane is cellulose diacetate film (CA), three cellulose acetate membrane (CTA), polysulfone membrane (PS), sulfonated polysulfone membrane (SPS), poly (ether sulfone) film (PES), sulfonated polyether sulfone film (SPES), polysulfonamides film (PSA), polyvinylidene fluoride film (PVDF), polyacrylonitrile film (PAN);

Preferred molecular cut off is 10000-70000,

Refined liquid after making with extra care in the step 2 can be carried out concentrate drying as required,

Wherein method for concentration is selected from: normal pressure concentrates, concentrating under reduced pressure, membrance concentration etc.

Membrance concentration: use reverse osmosis membrane to concentrate, fluid temperature 10-95 ℃, pressure 0-4.5MPa

Wherein drying means is selected from: constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying, far-infrared ray drying etc.

In the step 3,

The preferred drop pill of described preparation, its mesostroma is the drop pill of xylitol and starch eutectic mixture, method is as follows: medicine: substrate=1: 3-7, add Borneolum Syntheticum again, liquid paraffin is a condensing agent, 85 ℃-95 ℃ of medicine temperature, change 1-2 hour material time, condensate temperature-2-4 ℃, drip apart from 5-10cm, drip fast per minute 30-45 and drip.

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: medicine: substrate=1: 1-6, add Borneolum Syntheticum again, and the dimethyl-silicon oil coolant, the ice-water bath cooling, 75-85 ℃ of medicine temperature dripped apart from 5-10cm, drips fast per minute 20-30 and drips.

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, and method is as follows:

Medicine: substrate=1: 1-6, add Borneolum Syntheticum again, liquid paraffin coolant, condensate temperature are below 10 ℃, and 75-85 ℃ of medicine temperature dripped apart from 5-10cm, drips fast per minute 15-25 and drips.

The most preferred preparation method of the present invention is as follows:

In the step 1,

Extracting mode is if adopt decocting method, and method is as follows: with 8 times water extraction of medical material weight, be 8 if use aqueous alkali, its pH value; Extraction time is 2 times, 2 hours for the first time, and 1 hour for the second time.

Extracting mode is if adopt the warm macerating method, and method is as follows: the aqueous solvent with 20 times of medical material weight is soaked, and is 8 if use aqueous alkali, its pH value; Temperature is 90 ℃ of warm macerating 3 hours.

Extracting mode is if adopt continuous dynamic countercurrent extraction, and method is as follows: pulverizing medicinal materials 1-8mm, and add 2 times of water and run through, be respectively charged into 5 extraction pot, 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, are 8 if use aqueous alkali, its pH value; Circulation countercurrent extraction 1.5 hours extracts 3 times.

Extracting mode is if adopt microwave extraction, and method is as follows: the aqueous solvent with 12 times of medical material weight is carried out microwave extraction, as if being 8-9 with the aqueous alkali pH value; 1 hour extraction time, microwave power 320W.

Extracting mode is if adopt reflux, extract,, and method is as follows:

With the aqueous solvent reflux, extract, of 10 times of medical material weight, if be 8 with the aqueous alkali pH value; Extract 2 times, 2 hours for the first time, 1 hour for the second time.

Extracting mode is if adopt percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with the aqueous solvent of 15 times of medical material weight, if be 8 with the aqueous alkali pH value; Flooded percolation 3ml/ minute 24 hours.

Extracting mode is if adopt hyperpressure extraction, and method is as follows: medical material is crossed 20 orders, pressure 300MPa, and dwell time 5min extracts with the aqueous solvent of 10 times of medical material weight, if be 8 with the aqueous alkali pH value, soak time 16 hours.

Be preferably viscozyme L enzyme preparation, 50 ℃, pH value 4.6, medical material granularity 20-35 order, enzyme dosage 1: 200, first step enzymolysis and extraction time 80min, solid-to-liquid ratio 1: 15, second water is carried time 40min, solid-to-liquid ratio 1: 9.

In the step 2, after extracting solution was used the gauze coarse filtration, extracting solution did not concentrate, and used ultrafilter membrane is selected from: molecular cut off is the ultrafilter membrane of 20000-50000, uses cross flow filter; Ultrafiltrate is condensed into extractum or drying.

Described concentrate drying preferably spray drying: 180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10.

Lyophilization: pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1cm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.

Microwave drying: power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.

In the step 3,

The preferred drop pill of described preparation, its mesostroma is the drop pill of xylitol and starch eutectic mixture, method is as follows: optimized parameter: medicine: substrate=1: 5.5, add Borneolum Syntheticum again, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature, change 1.5 hours material time, 1 ℃ of condensate temperature drips apart from 7cm, drips 35 of fast per minutes.

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: optimized parameter: medicine: substrate=1: 3, add Borneolum Syntheticum again, and the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes.

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, method is as follows: optimized parameter: medicine: substrate=1: 3, add Borneolum Syntheticum again, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

The most preferred preparation method of other the present invention is listed in the embodiment of the invention.

The present invention is more more superior than prior art because of the change of technology causes the present invention to treat the medicine of coronary heart disease.

Following experimental data is used to illustrate effect of the present invention: experimental drug treatment group is the medicine of the embodiment of the invention 1.Matched group is the FUFANG DANSHEN DIWAN with prior art for preparing.

The comparison of medicinal dropping ball aspect the angina pectoris treatment effect of table 1 treatment coronary heart disease

Group	n	Produce effects	Effectively	Invalid	Increase the weight of	Total effective rate/%
							The treatment group	50	20	28	2	0	96①
Matched group	50	8	29	12	1	74

Annotate: 1. compare, analyze U=3.039, P＜0.01 through Radit with matched group.

The comparison of medicinal dropping ball aspect ECG curative effect of table 2 treatment coronary heart disease

Group	n	Produce effects	Effectively	Invalid	Increase the weight of	Total effective rate/%
							The treatment group	50	11	20	19	0	62①
Matched group	50	2	17	29	2	38

Annotate: 1. compare, analyze U=2.685, P＜0.01 through Radit with matched group.

Hemorheology index variation before and after the medicinal dropping ball treatment of table 3 treatment coronary heart disease (x ± s)

The medicinal dropping ball medication left ventricular contractile function comparison of table 4 treatment coronary heart disease (x ± s)

Annotate: 1. compare P＞0.05 before and after the medication; 2. compare P＜0.05 before and after the medication.

Efficient, quick-acting, the multi-purpose pure Chinese medicine dripping pills preparation that the present invention adopts the modern pharmacy technology to develop, prevent and treat the coronary heart disease determined curative effect, and can improve the blood capillary circulation,, comprise that diabetic renal papillary necrosis and diabetic nephropathy all have good preventive and therapeutic effect the diabetes microvascular complication.

The present invention carries out the refining of effective ingredient to extract, and quality is improved significantly, and is more prone to detect and control, and the content and the pharmaceutically active of active ingredient also are improved.

The invention has the advantages that: this process route can be realized industrialization, and is stable and controllable for quality.Relevant test shows, the present invention is than prior art curative effect height, the product purity height, and good absorbing, steady quality, the purposes novelty, preparation method technology is simple, easy to operate, with low cost simultaneously, is fit to suitability for industrialized production.

The specific embodiment

Specify the present invention with embodiment below, embodiment is for the ease of understanding the present invention, and claim that does not limit the present invention in any way and core content.

Embodiment 1

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%.

Preparation method:

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are extracted 2 times with the water boil of 8 times of weight, and 2 hours for the first time, 1 hour for the second time, mixed extract.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 40000 cellulose diacetate film excessively, adopts cross flow filter; Ultrafiltrate concentrates.

Step 3, with extract and Borneolum Syntheticum together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 5.5 adds Borneolum Syntheticum, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes and be prepared into drop pill.

Embodiment 2

Prescription: Radix Salviae Miltiorrhizae 20%, Radix Notoginseng 79%, Borneolum Syntheticum 1%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the water extraction of 2 times of weight, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 60000 cellulose diacetate film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 5, and the ultrafiltrate normal pressure concentrates, drying.

Step 3, with step 2 extract and Borneolum Syntheticum together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, liquid paraffin is a condensing agent, 85 ℃ of medicine temperature are changed 1 hour material time, condensate temperature-2 ℃, drip apart from 5cm, drip 30 of fast per minutes.

Embodiment 3

Prescription: Radix Salviae Miltiorrhizae 97%, Radix Notoginseng 2%, Borneolum Syntheticum 1.0%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the water extraction of 12 times of weight, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 80000 cellulose diacetate film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 9, and the ultrafiltrate normal pressure concentrates, drying.

Step 3, with step 2 extract and Borneolum Syntheticum together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 7 adds Borneolum Syntheticum, liquid paraffin is a condensing agent, 95 ℃ of medicine temperature are changed 2 hours material time, 4 ℃ of condensate temperatures, drip apart from 10cm, drip 45 of fast per minutes.

Embodiment 4

Prescription: Radix Salviae Miltiorrhizae 80%, Radix Notoginseng 19.8%, Borneolum Syntheticum 0.2%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the water extraction of 12 times of weight, and extraction time is 3 times, extracts mixed extract 3 hours at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 60000 three cellulose acetate membrane excessively, adopts dead-end filtration; The pH value of feed liquid is controlled at 9, ultrafiltrate concentrating under reduced pressure, drying.

Step 3 uses step 2 extract and Borneolum Syntheticum together as active constituents of medicine, and substrate is pool Luo Samu, and rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, dimethyl-silicon oil coolant, ice-water bath cooling, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 20 of fast per minutes.

Embodiment 5

Prescription: Radix Salviae Miltiorrhizae 27%, Radix Notoginseng 70%, Borneolum Syntheticum 3.0%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the potass extraction of 2 times of weight, and the aqueous alkali pH value is 7, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 80000 polysulfone membrane excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 6, and the ultrafiltrate normal pressure concentrates, drying.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is pool Luo Samu, and rate of charge is: the extract medicine: substrate=1: 6, add Borneolum Syntheticum, dimethyl-silicon oil coolant, ice-water bath cooling, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 30 of fast per minutes.

Embodiment 6

Prescription: Radix Salviae Miltiorrhizae 94%, Radix Notoginseng 4%, Borneolum Syntheticum 2%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with the potass extraction of 2 times of weight, and the aqueous alkali pH value is 10, and extraction time is 2 times, extracts mixed extract 1 hour at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 sulfonated polysulfone membrane excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 7, and the ultrafiltrate normal pressure concentrates, drying.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate for the PEG1000 rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 7

Prescription: Radix Salviae Miltiorrhizae 63%, Radix Notoginseng 35%, Borneolum Syntheticum 2%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are soaked with the aqueous solvent of 10 times of weight, and temperature is 75 ℃ of warm macerating 2 hours.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 70000 poly (ether sulfone) film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 7, and the ultrafiltrate membrance concentration uses reverse osmosis membrane to concentrate, and fluid temperature 10-95 ℃, pressure 0-4.5MPa, drying.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG1000, rate of charge is: the extract medicine: substrate=1: 6 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 8

Prescription: Radix Salviae Miltiorrhizae 69.5%, Radix Notoginseng 30%, Borneolum Syntheticum 0.5%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are soaked with the aqueous solvent of 30 times of weight, and temperature is 95 ℃ of warm macerating 4 hours.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 sulfonated polyether sulfone film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 6, and the ultrafiltrate normal pressure concentrates, spray drying.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG4000, and rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 9

Prescription: Radix Salviae Miltiorrhizae 90%, Radix Notoginseng 9%, Borneolum Syntheticum 1%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing add water and run through, and are respectively charged into 3 extraction pot, Continuous Countercurrent Extraction, with the aqueous solvent of 3 times of medical material weight, circulation countercurrent extraction 0.5 hour extracts 2 times.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polysulfonamides film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 5, and the ultrafiltrate normal pressure concentrates, spray drying.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG4000, rate of charge is: the extract medicine: substrate=1: 6 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 10

Prescription: Radix Salviae Miltiorrhizae 75.2%, Radix Notoginseng 23.5%, Borneolum Syntheticum 1.3%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing add water and run through, and are respectively charged into 7 extraction pot, Continuous Countercurrent Extraction, with the aqueous solvent of 8 times of medical material weight, circulation countercurrent extraction 3 hours extracts 4 times.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyvinylidene fluoride film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 6, and the ultrafiltrate normal pressure concentrates, lyophilization.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG6000, and rate of charge is: the extract medicine: substrate=1: 1, add Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 11

Prescription: Radix Salviae Miltiorrhizae 78.5%, Radix Notoginseng 21%, volatile oil of Lignum Dalbergiae Odoriferae 0.5%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng carry out microwave extraction with the aqueous solvent of 6 times of weight, 0.5 hour extraction time, microwave power 100W.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyvinylidene fluoride film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 7, and the ultrafiltrate normal pressure concentrates, microwave drying.

Step 3, extract that obtains with step 2 and volatile oil of Lignum Dalbergiae Odoriferae are together as active constituents of medicine, substrate is PEG6000, rate of charge is: the extract medicine: substrate=1: 6 adds volatile oil of Lignum Dalbergiae Odoriferae, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 12

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Styrax 0.92%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng carry out microwave extraction with the aqueous solvent of 18 times of weight, 2 hours extraction times, microwave power 500W.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyvinylidene fluoride film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 5, and the ultrafiltrate normal pressure concentrates, far-infrared ray drying.

Step 3, extract that obtains with step 2 and Styrax are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 1 adds Styrax, the liquid paraffin coolant, condensate temperature is below 10 ℃, 75 ℃ of medicine temperature are dripped apart from 5cm, drip 15 of fast per minutes.

Embodiment 13

Prescription: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Rhizoma Chuanxiong extract 0.92%.

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng carry out microwave extraction with the aqueous alkali of 18 times of weight, and the aqueous alkali pH value is 7-10; 2 hours extraction times, microwave power 500W.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyacrylonitrile film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 5, and the ultrafiltrate normal pressure concentrates, far-infrared ray drying.

Step 3, extract that obtains with step 2 and Rhizoma Chuanxiong extract are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 6 adds Rhizoma Chuanxiong extract, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 14

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng extract 2 times with 2 times aqueous solvent reflux, extract,, extract 1 hour at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyacrylonitrile film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 8, and the ultrafiltrate normal pressure concentrates, far-infrared ray drying.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG10000, rate of charge is: the extract medicine: substrate=1: 6 adds Borneolum Syntheticum, the liquid paraffin coolant, condensate temperature is below 10 ℃, 85 ℃ of medicine temperature are dripped apart from 10cm, drip 25 of fast per minutes.

Embodiment 15

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with 12 times aqueous alkali reflux, extract,, and the aqueous alkali pH value is 7; Extract 3 times, extracted 3 hours at every turn.

Embodiment 16

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are with 10 times of aqueous alkali reflux, extract, doubly, and the aqueous alkali pH value is 10; Extract 3 times, extracted 1 hour at every turn.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyacrylonitrile film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 9, and the ultrafiltrate normal pressure concentrates, far-infrared ray drying.

Embodiment 17

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing, with the alkaline soak of 10 times of described medical material weight, the aqueous alkali pH value is 7; Flooded percolation 2ml/ minute 24 hours.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyacrylonitrile film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 7, and the ultrafiltrate normal pressure concentrates, far-infrared ray drying.

Embodiment 18

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng coarse crushing, with the alkaline soak of 20 times of described medical material weight, the aqueous alkali pH value is 10; Flooded percolation 5ml/ minute 24 hours.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 10000 polyacrylonitrile film excessively, adopts cross flow filter; The pH value of feed liquid is controlled at 6, and the ultrafiltrate normal pressure concentrates, far-infrared ray drying.

Embodiment 19

Step 1, hyperpressure extraction: Radix Salviae Miltiorrhizae, Radix Notoginseng cross 10 orders, pressure 200MPa, dwell time 3min, the potass extraction that the weight of usefulness Radix Salviae Miltiorrhizae and Radix Notoginseng is 6 times, the aqueous alkali pH value is 7, soak time 12 hours.

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, substrate is the drop pill of pool Luo Samu, rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes.

Embodiment 20

Step 1, hyperpressure extraction: Radix Salviae Miltiorrhizae, Radix Notoginseng cross 40 orders, pressure 500MPa, dwell time 10min, the potass extraction that usefulness Radix Salviae Miltiorrhizae and three seven weight are 18 times, the aqueous alkali pH value is 10, soak time 24 hours.

Embodiment 21

Step 1, extracting mode is if adopt the enzyme process assisted extraction, and method is as follows:

Be selected from following enzyme preparation, viscozyme L enzyme preparation, alkaline protease, cellulase, hemicellulase.

Embodiment 22

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are soaked with the aqueous solvent of 20 times of weight, and temperature is 90 ℃ of warm macerating 3 hours.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 20000 cellulose diacetate film excessively, adopts cross flow filter; The ultrafiltrate concentrating under reduced pressure becomes extractum.

Embodiment 23

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng are pulverized 1-8mm, add 2 times of water and run through, and are respectively charged into 5 each extraction pot, and 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, and circulation countercurrent extraction 1.5 hours extracts 3 times.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 cellulose diacetate film excessively, adopts cross flow filter; The ultrafiltrate concentrating under reduced pressure becomes extractum.

Embodiment 24

Step 1 is carried out microwave extraction with the aqueous solvent of 12 times of Radix Salviae Miltiorrhizaes, pseudo-ginseng weight, if be 8-9 with the aqueous alkali pH value; 1 hour extraction time, microwave power 320W.

Embodiment 25

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are used the aqueous solvent reflux, extract, of 10 times of medical material weight, extract 2 times, 2 hours for the first time, 1 hour for the second time

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 cellulose diacetate film excessively, adopts cross flow filter; Ultrafiltrate is condensed into extractum.

Embodiment 26

Step 1, Radix Salviae Miltiorrhizae, Radix Notoginseng are used the aqueous alkali reflux, extract, of 10 times of medical material weight, and aqueous alkali ph=8 extracts 2 times, and 2 hours for the first time, 1 hour for the second time

Embodiment 27

Prescription: Radix Salviae Miltiorrhizae 77%, Radix Notoginseng 22%, Borneolum Syntheticum 1%.

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng coarse crushing are soaked with the aqueous solvent of 15 times of medical material weight, flood percolation 3ml/ minute 24 hours.

Embodiment 28

Prescription: Radix Salviae Miltiorrhizae 77%, Radix Notoginseng 22%, volatile oil of Lignum Dalbergiae Odoriferae 1%.

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng coarse crushing, with the alkaline soak of 15 times of medical material weight, the aqueous alkali pH value is 8; Flooded percolation 3ml/ minute 24 hours.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 cellulose diacetate film excessively, adopts cross flow filter; Concentrating under reduced pressure becomes extractum.

Step 3, with extract and volatile oil of Lignum Dalbergiae Odoriferae together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 5.5 adds volatile oil of Lignum Dalbergiae Odoriferae, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes and be prepared into drop pill.

Embodiment 29

Prescription: Radix Salviae Miltiorrhizae 77%, Radix Notoginseng 22%, Styrax 1%.

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng are crossed 20 orders, pressure 300MPa, dwell time 5min extracts with the aqueous solvent of 10 times of medical material weight, if be 8 with the aqueous alkali pH value, soak time 16 hours.

Step 3, with extract and Styrax together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 5.5 adds Styrax, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes and be prepared into drop pill.

Embodiment 30

Prescription: Radix Salviae Miltiorrhizae 77%, Radix Notoginseng 22%, Rhizoma Chuanxiong extract 1%.

Step 1, Radix Salviae Miltiorrhizae, pseudo-ginseng are crossed 20 orders, pressure 300MPa, dwell time 5min, with the potass extraction of 10 times of medical material weight, the aqueous alkali pH value is 8, soak time 16 hours.

Step 3, with extract and Rhizoma Chuanxiong extract together as active constituents of medicine, substrate is xylitol and starch eutectic mixture, and rate of charge is: the extract medicine: substrate=1: 5.5 adds Rhizoma Chuanxiong extract, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes and be prepared into drop pill.

Embodiment 31

Prescription: Radix Salviae Miltiorrhizae Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%.

Step 1 adopts the enzyme process assisted extraction: viscozyme L enzyme preparation, 50 ℃, pH value 4.6, Radix Salviae Miltiorrhizae, pseudo-ginseng granularity 20-35 order, medical material: enzyme dosage 1: 200, first step enzymolysis and extraction time 80min, solid-liquid weight ratio 1: 15, second water is carried time 40min, solid-liquid weight ratio 1: 9.

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 cellulose diacetate film excessively, adopts cross flow filter; Ultrafiltrate concentrates.

Embodiment 32

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 three cellulose acetate membrane excessively, adopts cross flow filter; Ultrafiltrate is condensed into extractum.

Embodiment 33

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 polysulfone membrane excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 34

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 sulfonated polysulfone membrane excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 35

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 poly (ether sulfone) film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 36

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 sulfonated polyether sulfone film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 37

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 polysulfonamides film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is for being PEG1000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 38

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 polyvinylidene fluoride film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 39

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 40

Step 2, extracting solution gauze coarse filtration, molecular cut off is 30000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 41

Step 2, extracting solution gauze coarse filtration, molecular cut off is 40000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, spray drying (180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10).

Embodiment 42

Step 2, extracting solution gauze coarse filtration, molecular cut off is 40000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, lyophilization (pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.)

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is for being PEG4000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 43

Step 2, extracting solution gauze coarse filtration, molecular cut off is 30000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, lyophilization (pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1mm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃.)

Embodiment 44

Step 2, extracting solution gauze coarse filtration, molecular cut off is 30000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Embodiment 45

Step 2, extracting solution gauze coarse filtration, molecular cut off is 20000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is for being PEG6000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 46

Step 2, extracting solution gauze coarse filtration, molecular cut off is 40000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Embodiment 47

Step 2, extracting solution gauze coarse filtration, molecular cut off is 50000 polyacrylonitrile film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Embodiment 48

Step 2, extracting solution gauze coarse filtration, molecular cut off is 30000 sulfonated polyether sulfone film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Step 3, extract that obtains with step 2 and Borneolum Syntheticum are together as active constituents of medicine, and substrate is PEG6000, and rate of charge is: the extract medicine: substrate=1: 3, add Borneolum Syntheticum, liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 49

Step 1, Radix Salviae Miltiorrhizae and pseudo-ginseng pulverize separately 1-8mm add 2 times of water and run through, and are respectively charged into 5 extraction pot, and 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, and circulation countercurrent extraction 1.5 hours extracts 3 times.

Step 2, Radix Salviae Miltiorrhizae and Radix Notoginseng extracting solution are used the gauze coarse filtration respectively, and molecular cut off is 40000 polysulfonamides film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Step 3, Radix Salviae Miltiorrhizae that obtains with step 2 and Radix Notoginseng extract and Borneolum Syntheticum are together as active constituents of medicine, substrate is for being PEG6000, rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 50

Step 1, Radix Salviae Miltiorrhizae and Radix Notoginseng are soaked with the aqueous solvent of 20 times of weight respectively, and temperature is 90 ℃ of warm macerating 3 hours.

Step 2, Radix Salviae Miltiorrhizae and Radix Notoginseng extracting solution are used the gauze coarse filtration respectively, and molecular cut off is 30000 polysulfonamides film excessively, adopts cross flow filter; Ultrafiltrate concentrates, microwave drying (power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40.)

Step 3, Radix Salviae Miltiorrhizae that obtains with step 2 and Radix Notoginseng extract and Borneolum Syntheticum are together as active constituents of medicine, substrate is PEG6000, rate of charge is: the extract medicine: substrate=1: 3 adds Borneolum Syntheticum, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.

Embodiment 51

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make tablet.

Embodiment 52

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make capsule.

Embodiment 53

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make granule.

Embodiment 54

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make powder.

Embodiment 55

According to any one embodiment among the embodiment 1-50, add conventional adjuvant, use the conventional method on the galenic pharmacy, make pill.

Claims

1. compound red sage root preparation is characterized in that prescription consists of: Radix Salviae Miltiorrhizae 20%-97%, and Radix Notoginseng 2.0%-79%, Borneolum Syntheticum 0.2%-3.0%, its preparation method is as follows:

Step 2, the extracting solution ultrafiltration obtains extract;

Step 3 together as active constituents of medicine, is prepared into pharmaceutical preparation with above-mentioned extract and Borneolum Syntheticum.

2. the preparation of claim 1 is characterized in that, prescription consists of: Radix Salviae Miltiorrhizae 63.0%-94%, and Radix Notoginseng 4.0%-35.0%, Borneolum Syntheticum 0.5%-2.0%, its preparation method is as follows:

In the step 1, described Radix Salviae Miltiorrhizae, pseudo-ginseng are medical material, decoction pieces or the powder of Radix Salviae Miltiorrhizae, Radix Notoginseng, and the described water that is extracted as is carried, and described extraction adopts Radix Salviae Miltiorrhizae, panax mixed to extract, or Radix Salviae Miltiorrhizae, Radix Notoginseng extract separately respectively, extract the back and mix;

In the step 2, described ultrafiltration: be that extracting solution is purified through ultrafiltration membrance filter;

In the step 3, the extract of step 2 and Borneolum Syntheticum are prepared into dropping pill formulation together as active constituents of medicine.

3. the preparation of claim 1 is characterized in that, prescription consists of: Radix Salviae Miltiorrhizae 75.2%-90%, and Radix Notoginseng 9%-23.5%, Borneolum Syntheticum 0.5%-1.3%, its preparation method is as follows:

In the step 1, described extracting mode is selected from decocting method, warm macerating method, enzyme process assisted extraction, continuous dynamic countercurrent extraction, reflux, extract,, percolation extraction, microwave extraction or hyperpressure extraction,

In the step 2, described ultrafiltration: be with extracting solution ultrafiltration remove impurity after predefecation is handled,

Predefecation is handled and is adopted the independent or use in conjunction of following method: carry out coarse filtration with gauze, tiffany; Carry out microfiltration with ceramic membrane; Separation of supernatant behind high speed centrifugation;

Ultrafilter membrane is selected from cellulose diacetate film, three cellulose acetate membrane, cyanoethyl cellulose film, polysulfone membrane, sulfonated polysulfone membrane, poly (ether sulfone) film, sulfonated polyether sulfone film, polysulfonamides film, phenolphthalein side group polyarylsulfone (PAS) film, polyvinylidene fluoride film, polyacrylonitrile film, polyimide film, cellulose membrane, methyl methacrylate-acrylonitrile copolymer film, polyacrylonitrile/cellulose diacetate blend film, the dynamic ultrafilter membrane that forms, and the Modified Membrane of above-mentioned film

Refined liquid after refining is through concentrating or/and drying obtains extract, and extract is extractum or powder;

Method for concentration is selected from that normal pressure concentrates, concentrating under reduced pressure or membrance concentration,

Drying means is selected from constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying or far-infrared ray drying,

In the step 3, described dropping pill formulation, the adjuvant that adopts during preparation is selected from: water-soluble base has Polyethylene Glycol, polyoxyethylene monostearate, poloxamer, sodium stearate, glycerin gelatine; Xylitol and composites of starch, erythritol, the drop pill medicine is auxilliary than being 1: 1-6; The drop pill condensing agent that adopts during preparation is selected from: liquid paraffin, dimethicone, Semen Maydis oil, kerosene, vegetable oil or its mixture.

4. the preparation of claim 3 is characterized in that, prescription consists of: Radix Salviae Miltiorrhizae 82.87%, and Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%, its preparation method is as follows:

In the step 1,

Extracting mode adopts decocting method, and method is as follows: with the 2-12 water extraction doubly of medical material weight, be 7-10 if use aqueous alkali, its pH value; Extraction time is 1-3 time, extracts 1-3 hour at every turn;

Extracting mode adopts the warm macerating method, and method is as follows: soaking with medical material weight 10-30 aqueous solvent doubly, is 7-10 if use aqueous alkali, its pH value; Temperature be 75 ℃-95 ℃ warm macerating 2-4 hour;

Extracting mode adopts continuous dynamic countercurrent extraction, and method is as follows: the medical material coarse crushing, and add water and run through, be respectively charged into 3-7 extraction pot, Continuous Countercurrent Extraction with medical material weight 3-8 aqueous solvent doubly, is 7-10 if use aqueous alkali, its pH value; Circulation countercurrent extraction 0.5-3 hour extracts 2-4 time;

Extracting mode adopts microwave extraction, and method is as follows: carry out microwave extraction with medical material weight 6-18 aqueous solvent doubly, as if being 7-10 with the aqueous alkali pH value; Extraction time 0.5-2 hour, microwave power 100-500W;

Extracting mode adopts reflux, extract,, and method is as follows:

With medical material weight 2-12 aqueous solvent reflux, extract, doubly, if be 7-10 with the aqueous alkali pH value; Extract 1-3 time, extracted 1-3 hour at every turn;

Extracting mode adopts percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with medical material weight 10-20 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value; Flooded percolation 2-5ml/ minute 24 hours;

Extracting mode adopts hyperpressure extraction, and method is as follows: medical material is crossed the 10-40 order, pressure 200-500MPa, and dwell time 3-10min extracts with medical material weight 6-18 aqueous solvent doubly, if be 7-10 with the aqueous alkali pH value, soak time 12-24 hour;

Enzyme preparation is selected from, viscozyme L enzyme preparation, alkaline protease, cellulase, hemicellulase;

In the step 2,

Refining mode adopts ultrafiltration, and method is as follows: adopting the molecular weight that dams is the ultrafilter membrane of 6000-80000; Cross flow filter or dead-end filtration are adopted in ultrafiltration; The pH value of feed liquid is controlled at 5-9;

Ultrafilter membrane is cellulose diacetate film, three cellulose acetate membrane, polysulfone membrane, sulfonated polysulfone membrane, poly (ether sulfone) film, sulfonated polyether sulfone film, polysulfonamides film, polyvinylidene fluoride film, polyacrylonitrile film;

Refined liquid after making with extra care in the step 2 is carried out concentrate drying as required,

Wherein method for concentration is selected from: normal pressure concentrates, concentrating under reduced pressure or membrance concentration;

Described membrance concentration: use reverse osmosis membrane to concentrate, fluid temperature 10-95 ℃, pressure 0-4.5MPa

Wherein ten drying methods are selected from: constant pressure and dry, drying under reduced pressure, spray drying, lyophilization, microwave drying or far-infrared ray drying;

In the step 3,

Described preparation drop pill, its mesostroma is the drop pill of xylitol and starch eutectic mixture, preparation method is: medicine and substrate are mixed, and medicine: substrate=1: 3-7 adds Borneolum Syntheticum again, liquid paraffin is a condensing agent, 85 ℃-95 ℃ of medicine temperature are changed 1-2 hour material time, condensate temperature-2-4 ℃, drip apart from 5-10cm, drip fast per minute 30-45 and drip;

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: medicine and substrate are mixed, medicine: substrate=1: 1-6, add Borneolum Syntheticum again, and the dimethyl-silicon oil coolant, the ice-water bath cooling, 75-85 ℃ of medicine temperature dripped apart from 5-10cm, drips fast per minute 20-30 and drips;

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, method is as follows: medicine and substrate are mixed, medicine: substrate=1: 1-6, add Borneolum Syntheticum again, liquid paraffin coolant, condensate temperature be below 10 ℃, 75-85 ℃ of medicine temperature, drip apart from 5-10cm, drip fast per minute 15-25 and drip.

5. the preparation of claim 4 is characterized in that, wherein:

In the step 1,

Extracting mode adopts decocting method, and method is as follows: with the water extraction of 8 times of medical material weight, be 8 if use aqueous alkali, its pH value; Extraction time is 2 times, 2 hours for the first time, and 1 hour for the second time;

Extracting mode adopts the warm macerating method, and method is as follows: the aqueous solvent with 20 times of medical material weight is soaked, and is 8 if use aqueous alkali, its pH value; Temperature is 90 ℃ of warm macerating 3 hours;

Extracting mode adopts continuous dynamic countercurrent extraction, and method is as follows: pulverizing medicinal materials 1-8mm, and add 2 times of water and run through, be respectively charged into 5 extraction pot, 5 groups of Continuous Countercurrent Extraction add the aqueous solvent of 4 times of medical material weight, are 8 if use aqueous alkali, its pH value; Circulation countercurrent extraction 1.5 hours extracts 3 times;

Extracting mode adopts microwave extraction, and method is as follows: the aqueous solvent with 12 times of medical material weight is carried out microwave extraction, as if being 8-9 with the aqueous alkali pH value; 1 hour extraction time, microwave power 320W;

Extracting mode adopts reflux, extract,, and method is as follows:

With the aqueous solvent reflux, extract, of 10 times of medical material weight, if be 8 with the aqueous alkali pH value; Extract 2 times, 2 hours for the first time, 1 hour for the second time;

Extracting mode adopts percolation to extract, and method is as follows:

The medical material coarse crushing is soaked with the aqueous solvent of 15 times of medical material weight, if be 8 with the aqueous alkali pH value; Flooded percolation 3ml/ minute 24 hours;

Extracting mode adopts hyperpressure extraction, and method is as follows: medical material is crossed 20 orders, pressure 300MPa, and dwell time 5min extracts with the aqueous solvent of 10 times of medical material weight, if be 8 with the aqueous alkali pH value, soak time 16 hours;

Extracting mode adopts the enzyme process assisted extraction, and method is as follows:

With viscozyme L enzyme preparation, 50 ℃ of temperature, pH value 4.6, medical material granularity 20-35 order, enzyme dosage 1: 200, first step enzymolysis and extraction time 80min, solid-to-liquid ratio 1: 15, the second step water is carried time 40min, solid-to-liquid ratio 1: 9;

In the step 2, extracting solution is without concentrating, direct ultra-filtration, and used ultrafilter membrane molecular cut off is the ultrafilter membrane of 20000-50000, uses cross flow filter; Ultrafiltrate is condensed into extractum or is dried to powder;

Described drying is a spray drying: 180 ℃ of inlet temperature, 80 ℃ of leaving air temps, feed liquid proportion 1.10; Lyophilization: pre-freeze temperature-40 ℃, vacuum 0.08Mpa, material thickness 1cm, 35 ℃ of temperature of heating plate, condenser temperature-40 ℃; Microwave drying: power 15.3kw, temperature 40-70 ℃, vacuum 0.02-0.08MPa, medicinal liquid proportion 1.20-1.40;

In the step 3,

Described preparation drop pill, its mesostroma is the drop pill of xylitol and starch eutectic mixture, method is as follows: medicine and substrate are mixed, and medicine: substrate=1: 5.5 adds Borneolum Syntheticum again, liquid paraffin is a condensing agent, 90 ℃ of medicine temperature are changed 1.5 hours material time, 1 ℃ of condensate temperature, drip apart from 7cm, drip 35 of fast per minutes;

Its mesostroma is the drop pill of pool Luo Samu, and method is as follows: medicine and substrate are mixed, medicine: substrate=1: 3, add Borneolum Syntheticum again, and the dimethicone condensing agent, the ice-water bath cooling, 80 ℃ of medicine temperature are dripped apart from 7cm, drip 25 of fast per minutes;

Its mesostroma is PEG1000, PEG4000, PEG6000, PEG10000 or the blended drop pill of several substrate, method is as follows: medicine and substrate are mixed, medicine: substrate=1: 3, add Borneolum Syntheticum again, the liquid paraffin condensing agent, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature, drip apart from 6cm, drip 20 of fast per minutes.

6. the preparation of claim 1, it is characterized in that, wherein: the pharmaceutical formulation of claim 1 is that the blood circulation promoting and blood stasis dispelling according to the traditional Chinese medical science, the effect of regulating QI to relieve pain are determined, situation according to patient, wherein Borneolum Syntheticum can be with being selected from volatile oil of Lignum Dalbergiae Odoriferae, Styrax, Rhizoma Chuanxiong extract, or wherein any combination, or other invigorating blood circulation (breaking) silt, regulating QI to relieve pain medicine or pharmaceutical preparations substitute.

7. the preparation of claim 1, it is characterized in that wherein: preparation formulation is selected from: tablet, capsule, drop pill, pill, powder spray, aerosol, gel, injection, lyophilizing divide the quick releasing formulation or the durative action preparation of injection, Orally-disintegrating tablet and these dosage forms.

8. the application of the preparation of claim 1 in the medicine of preparation resisting coronary heart disease, angina pectoris cardiovascular disease.

9. the preparation method of the preparation of claim 1, wherein the compound red sage root preparation prescription is: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%, its preparation method is:

In the step 2, the described refining ultrafiltration that is, described ultrafiltration: be with extracting solution gauze coarse filtration, crossing molecular cut off is the ultrafilter membrane of 20000-50000, adopts cross flow filter; Ultrafiltrate concentrates;

10. the preparation method of claim 9, wherein

In the step 1,

Extracting mode adopts decocting method, and method is as follows: with the water extraction of 8 times of medical material weight, be 8 if use aqueous alkali, its pH value; Extraction time is 2 times, 2 hours for the first time, and 1 hour for the second time,

In the step 2,

Extracting solution is without concentrating, and the ultrafilter membrane molecular cut off is the ultrafilter membrane of 20000-50000, uses cross flow filter; Ultrafiltrate is condensed into extractum or drying;

In the step 3,

Substrate is PEG1000, PEG4000, PEG6000, PEG10000 or their mixing, and method is as follows: medicine and substrate are mixed, medicine: substrate=1: 3, add Borneolum Syntheticum again, use the liquid paraffin coolant, 5 ℃ of condensate temperatures, 80 ℃ of medicine temperature are dripped apart from 6cm, drip 20 of fast per minutes.