CN105748685B

CN105748685B - Beriberi bacteriostat and preparation method thereof

Info

Publication number: CN105748685B
Application number: CN201610215480.2A
Authority: CN
Inventors: 蒋勇; 罗霞娟; 李倩文; 谢兵; 时建伟
Original assignee: Yangtze Normal University
Current assignee: Yangtze Normal University
Priority date: 2016-04-08
Filing date: 2016-04-08
Publication date: 2020-06-05
Anticipated expiration: 2036-04-08
Also published as: CN105748685A

Abstract

The invention discloses a beriberi bacteriostat and a preparation method thereof. The bacteriostatic agent is prepared from the following Chinese medicinal material extracts in parts by mass: 1-5 parts of fructus cnidii, 1-6 parts of radix angelicae, 1-5 parts of cortex dictamni, 1-4 parts of cortex phellodendri, 1-5 parts of folium ginkgo and 1-5 parts of polygonum cuspidatum. The preparation method comprises pulverizing the above Chinese medicinal materials, soaking fructus Cnidii, cortex Dictamni Radicis, cortex Phellodendri and rhizoma Polygoni Cuspidati in ethanol solution, and ultrasonic extracting to obtain their respective extracts; performing supercritical extraction on radix Angelicae Dahuricae to obtain viscous pasty radix Angelicae Dahuricae extract; soaking folium Ginkgo in deionized water, and ultrasonic extracting to obtain folium Ginkgo water extract; and dissolving the extracts in an ethanol solution, adding chitosan and polyvinyl alcohol, and performing ultrasonic dissolution to obtain the beriberi bacteriostat. The product prepared by the method has the advantages of difficult relapse, no molting, good inhibitory effect on staphylococcus aureus and the like.

Description

Beriberi bacteriostat and preparation method thereof

Technical Field

The invention belongs to the technical field of medicines, particularly relates to the technical field of antibacterial bacteriostatic agents, and particularly relates to a beriberi bacteriostatic agent and a preparation method thereof.

Background

Beriberi, also known as tinea pedis, is a foot dermatosis caused by pathogenic fungi and is infectious. In China, the incidence of tinea pedis is also quite high. There is no sebaceous gland between foot and toe of human, so there is no fatty acid for inhibiting skin filamentous fungi, and physiological defense function is poor, while skin sweat glands of these parts are abundant, sweating is more, and air circulation is poor, local moisture and warmth are good for growth of filamentous fungi. Abuse of antibiotics, long-term use of corticosteroids, immunosuppressants, etc., also causes a dysregulation of the normal flora of the skin and increases susceptibility to athlete's foot. Clinically, it is manifested as blister between toes, desquamation or white, moist and soft skin, erosion, or skin thickening, roughness, cracking, spreading to the metatarsus and edge of foot, and itching.

The root for treating beriberi is to inhibit the growth and reproduction of fungi. At present, the medicaments for treating the beriberi sold in the market mainly comprise ketoconazole synthetic medicaments such as dactinoin, fendamine and the like, and Chinese medicinal preparations such as johnsonqing and the like. The dosage form of the Daktarin is paste or gel, and the use is not convenient. The formulation of the Weidaling is spray, is convenient to use, but has the problem of easy relapse after use. The Heshi tinea drug is a traditional Chinese medicine preparation, is convenient to use, and has the problem of easy relapse after use.

Therefore, the medicines for treating beriberi sold in the market at present have the problem of easy relapse after use. In addition, the research shows that staphylococcus aureus is also a large factor of dermatophytosis infection, but the current medicines and bacteriostatic agents cannot effectively inhibit the dermatophytosis infection.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the beriberi bacteriostat which is not easy to relapse, does not exuviate and has good effect of inhibiting staphylococcus aureus, and the preparation method thereof.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a beriberi bacteriostat is prepared from the following Chinese medicinal material extracts in parts by mass: 1-5 parts of fructus cnidii extract, 1-6 parts of radix angelicae extract, 1-5 parts of cortex dictamni extract, 1-4 parts of cortex phellodendri extract, 1-5 parts of folium ginkgo extract and 1-5 parts of polygonum cuspidatum extract.

Preferably, the following Chinese medicinal material extracts can be adjusted in parts by mass: 1 part of fructus cnidii extract, 1 part of radix angelicae extract, 1 part of cortex dictamni extract, 1 part of cortex phellodendri extract, 1 part of folium ginkgo extract and 1 part of polygonum cuspidatum extract.

The preparation method of the beriberi bacteriostat comprises the following steps:

1) crushing fructus cnidii, and mixing the crushed fructus cnidii with a solvent according to a mass-volume ratio of 1 g: soaking 5mL of the fructus cnidii in an ethanol solution, performing ultrasonic extraction for 1h, filtering to remove solid impurities, and performing reduced pressure concentration to obtain a thick paste-like fructus cnidii extract for later use;

2) pulverizing radix Angelicae Dahuricae, and performing supercritical extraction to obtain viscous paste-like radix Angelicae Dahuricae extract;

3) crushing the cortex dictamni, and mixing the materials according to a mass-volume ratio of 1 g: soaking 3mL of the extract in an ethanol solution, performing ultrasonic extraction for 1h, filtering to remove solid impurities, and concentrating the filtrate under reduced pressure to obtain a cortex dictamni extract for later use;

4) crushing cortex phellodendri, and mixing the materials according to a mass-volume ratio of 1 g: soaking 5mL of the extract in an ethanol solution, filtering to remove solid impurities, and concentrating the filtrate under reduced pressure to obtain a phellodendron extract for later use;

5) crushing ginkgo leaves, and mixing the crushed ginkgo leaves according to the mass volume ratio of 1 g: soaking 10mL of the ginkgo leaf extract in deionized water, performing ultrasonic extraction for 1h, filtering to remove solid impurities, and performing reduced pressure concentration, cooling and crystallization on the filtrate to obtain a ginkgo leaf water extract for later use;

6) crushing giant knotweed rhizome, and mixing the crushed giant knotweed rhizome with the following raw materials in a mass-volume ratio of 1 g: soaking 4mL of the polygonum cuspidatum in an ethanol solution, performing ultrasonic extraction for 1h, filtering to remove solid impurities, and concentrating the filtrate under reduced pressure to obtain a polygonum cuspidatum extract for later use;

7) respectively dissolving fructus Cnidii extract, radix Angelicae Dahuricae extract, cortex Dictamni Radicis extract, cortex Phellodendri extract, folium Ginkgo extract, and rhizoma Polygoni Cuspidati extract in ethanol solution at mass volume ratio of 1-5g:500mL, 1-6g:500mL, 1-5g:500mL, 1-4g:500mL, 1-5g:500mL, and 1-5g:500 mL; respectively adding the chitosan and the polyvinyl alcohol into the ethanol solution according to the mass-volume ratio of 2g to 500mL to the ethanol solution; then ultrasonic dissolving is carried out to obtain the beriberi bacteriostat.

Wherein the mass concentration of the ethanol solution in the step 1) is 70 percent; the mass concentration of the ethanol solution in the step 3) and the step 6) is 60 percent; the mass concentration of the ethanol solution in the step 4) is 50 percent; the mass concentration of the ethanol solution in the step 7) is 55%.

When the traditional Chinese medicinal materials are crushed, the traditional Chinese medicinal materials are crushed to 150-200 meshes, so that the traditional Chinese medicinal materials can fully act with ethanol, and effective components can be conveniently and efficiently extracted.

In addition, the beriberi bacteriostat provided by the invention can be further processed into dosage forms such as ointment, patch, spray and the like, so that the bacteriostat can be selectively used by patients according to conditions.

The beriberi bacteriostat is directly sprayed when in use, 2-3 times a day and 3-7 times a day can be cured.

The pharmacological actions of the traditional Chinese medicine are as follows:

fructus cnidii: pungent and bitter taste, warm nature; has little toxicity; it enters kidney meridian. Has effects in warming kidney, supporting yang, eliminating dampness, dispelling pathogenic wind, and killing parasite.

Radix angelicae: pungent taste and warm nature; it enters stomach, large intestine and lung meridians. Has effects in dispelling pathogenic wind, removing dampness, inducing resuscitation, relieving pain, relieving swelling, and expelling pus.

Cortex dictamni: also called cortex Dictamni Radicis. Bitter taste and cold nature; it enters spleen, stomach and bladder meridians. Has effects of clearing heat, eliminating dampness, dispelling pathogenic wind, and removing toxic substance. It is commonly used for damp-heat sores, yellow water dripping, eczema, rubella, scabies, sores, scabies, arthralgia due to wind-damp-heat, jaundice and dark urine.

Phellodendron bark: bitter taste and cold nature; it enters kidney and bladder meridians. Has effects in clearing away heat, eliminating dampness, purging pathogenic fire, removing steam, removing toxic materials, and treating sore. Can be used for treating dysentery due to damp-heat pathogen, jaundice, leukorrhagia, stranguria caused by heat pathogen, tinea pedis, flaccidity, hectic fever due to yin deficiency, night sweat, nocturnal emission, pyocutaneous disease, toxic swelling, eczema, and pruritus.

Ginkgo leaf: sweet, bitter and astringent taste, mild in nature; it enters heart and lung meridians. Has effects of astringing lung, relieving asthma, promoting blood circulation for removing blood stasis, and relieving pain.

Giant knotweed rhizome: slightly bitter taste and slightly cold nature; it enters liver, gallbladder and lung meridians. Has the effects of dispelling pathogenic wind, promoting diuresis, removing blood stasis, relieving pain, relieving cough and eliminating phlegm. It is commonly used for arthralgia, jaundice due to damp-heat pathogen, amenorrhea, abdominal mass, scald due to hot water or fire, traumatic injury, carbuncle, swelling, sore and toxin, cough and excessive phlegm.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention takes natural Chinese medicinal materials as raw materials, has good safety and small toxic and side effects.

2. The natural traditional Chinese medicine preparation has the advantages of obvious antibacterial effect, quick itching relieving, no molting, convenient use and difficult relapse after use.

3. The bacteriostatic agent prepared by the invention can effectively inhibit the growth and reproduction of staphylococcus aureus, and greatly reduce the infection caused by beriberi.

Detailed Description

The present invention will be described in further detail with reference to specific examples.

First, an embodiment

Example 1

Fructus Cnidii, radix Angelicae Dahuricae, cortex Dictamni Radicis, cortex Phellodendri, folium Ginkgo and rhizoma Polygoni Cuspidati each 100 g. The beriberi bacteriostat is prepared according to the following steps:

1) crushing fructus Cnidii, soaking in 500mL of 70% ethanol solution, ultrasonically extracting for 1h, filtering to remove solid impurities, and concentrating under reduced pressure to obtain thick paste fructus Cnidii extract;

3) pulverizing cortex Dictamni Radicis, soaking in 300mL of 60% solution B, ultrasonic extracting for 1h, filtering to remove solid impurities, and concentrating the filtrate under reduced pressure to obtain cortex Dictamni Radicis extract;

4) pulverizing cortex Phellodendri, soaking in 500mL 50% ethanol solution, ultrasonic extracting for 1 hr, filtering to remove solid impurities, and concentrating the filtrate under reduced pressure to obtain cortex Phellodendri extract

5) Crushing ginkgo leaves, soaking the crushed ginkgo leaves in 1000ml of deionized water, carrying out ultrasonic treatment for 1 hour, filtering to remove solid impurities, and carrying out reduced pressure concentration, cooling and crystallization on filtrate to obtain ginkgo leaf water extract;

6) crushing giant knotweed rhizome, soaking in 400mL of 60% ethanol solution with the mass concentration, performing ultrasonic extraction for 1h, filtering to remove solid impurities, and concentrating the filtrate under reduced pressure to obtain a giant knotweed rhizome extract;

7) adding 1g of each extract into 500mL of 60% ethanol solution, adding 2g of each chitosan and polyvinyl alcohol, and performing ultrasonic dissolution to obtain the beriberi bacteriostat.

Examples 2 and 3

The preparation method is the same as example 1, and the use weight of the traditional Chinese medicine extract in the step 7) is different, as shown in the table 1:

TABLE 1 weight of extract of Chinese medicinal materials of examples 2 and 3

The antibacterial and bacteriostatic experiments show that the bacteriostatic agent has the bacteriostatic rate of more than 99.9 percent on the trichophyton alba, the epidermophyton floccosum, the trichophyton rubrum, the trichophyton mentagrophytes and the trichophyton gypseum and the bacteriostatic rate of more than 85 percent on the staphylococcus aureus.

Second, clinical experiments

150 patients with beriberi are selected and averagely divided into 3 groups, the beriberi bacteriostat prepared by the above examples 1-3 is adopted for treatment, the bacteriostat generally takes effect within 24 hours after the application, and the total effect rate reaches 100 percent within 48 hours; after the medicine is taken for 72 hours, most patients can be cured, and after the medicine is taken for 7 days, all patients can be cured. Tracking for 3 months, and no recurrence. The statistical results are shown in table 2.

TABLE 2 statistical table of treatment effect

In table 2, the onset of action means that the blister and the skin are whitish, moist and soft, and no severe itching occurs; the recovery means that no blister, desquamation or skin whitening, dampness and softness exist, no severe itching exists, and no relapse occurs within 3 months after the medicine withdrawal is followed.

Three, typical cases

Typical case 1:

zhao, male 25 years old, suffered from pain and beriberi for 3 months, had blisters between toes and white, wet and soft skin, and had severe itching occasionally. When the traditional Chinese medicine composition is used for treatment in the embodiment 1, the phenomena of blisters and whitening, wetting and softening of skin are relieved within 24 hours from the first use, no severe itching exists, and blisters, desquamation or whitening, wetting and softening of skin can be healed after 3 days without severe itching. In order to consolidate the curative effect, the medicine is continuously used for 4 days, and the follow-up visit is followed for 6 months without relapse.

Typical case 2:

in 65 years old, male with this disease will suffer from pain and beriberi for 5 years, blister in toes, white, wet and soft skin, erosion and cracking of part of skin, and sometimes severe itching. When the medicine is used for treatment in the embodiment 3 of the invention, the phenomena of blisters and whitening, wetting and softening of skin are relieved within 36 hours from the first use, no severe itching exists, and blisters, desquamation or whitening, wetting and softening of skin can be healed after 5 days without severe itching. In order to consolidate the curative effect, the medicine is continuously used for 4 days, and the follow-up visit is followed for 6 months without relapse.

Typical case 4:

zheng for some women, age 41, with blisters between the toes, white, moist and soft skin, and some skin cracks and itching. When the medicine is used for treatment in the embodiment 2 of the invention, the phenomena of blisters and whitening, wetting and softening of skin are relieved within 48 hours from the first use, no severe itching exists, and blisters, desquamation or whitening, wetting and softening of skin can be healed after 3 days without severe itching. In order to consolidate the curative effect, the medicine is continuously used for 4 days, and the follow-up visit is followed for 6 months without relapse.

The above examples of the present invention are merely illustrative of the present invention and are not intended to limit the embodiments of the present invention. Variations and modifications in other variations will occur to those skilled in the art upon reading the foregoing description. Not all embodiments are exhaustive. All obvious changes and modifications of the present invention are within the scope of the present invention.

Claims

1. The beriberi bacteriostat is characterized by being prepared from the following traditional Chinese medicine extracts in parts by mass: 1-5 parts of fructus cnidii extract, 1-6 parts of radix angelicae extract, 1-5 parts of cortex dictamni extract, 1-4 parts of cortex phellodendri extract, 1-5 parts of folium ginkgo extract and 1-5 parts of polygonum cuspidatum extract, and further comprises chitosan and polyvinyl alcohol;

7) respectively dissolving fructus Cnidii extract, radix Angelicae Dahuricae extract, cortex Dictamni Radicis extract, cortex Phellodendri extract, folium Ginkgo extract, and rhizoma Polygoni Cuspidati extract in ethanol solution at mass volume ratio of 1-5g:500mL, 1-6g:500mL, 1-5g:500mL, 1-4g:500mL, 1-5g:500mL, and 1-5g:500 mL; respectively adding the chitosan and the polyvinyl alcohol into the ethanol solution according to the mass-volume ratio of 2g to 500mL to the ethanol solution; then ultrasonic dissolving is carried out to obtain the beriberi bacteriostat; the bacteriostatic agent has a bacteriostatic rate of over 99.9% on trichophyton alba, trichophyton floccosum, trichophyton rubrum, trichophyton mentagrophytes and trichophyton gypseum.

2. The beriberi bacteriostat according to claim 1, which is prepared from the following traditional Chinese medicine extracts in parts by mass: 1 part of fructus cnidii extract, 1 part of radix angelicae extract, 1 part of cortex dictamni extract, 1 part of cortex phellodendri extract, 1 part of folium ginkgo extract and 1 part of polygonum cuspidatum extract.

3. The dermatophytosis bacteriostatic agent according to claim 1, wherein the mass concentration of the ethanol solution in the step 1) is 70%; the mass concentration of the ethanol solution in the step 3) and the step 6) is 60 percent; the mass concentration of the ethanol solution in the step 4) is 50 percent; the mass concentration of the ethanol solution in the step 7) is 55%.